LOCUS       DASZOB010000001         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_21,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000001 DASZOB010000000
VERSION     DASZOB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VG709_00005"
     CDS             <1..1266
                     /locus_tag="VG709_00005"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWC31494.1"
                     /translation="VQTASVGETCAEAKRAARTLARLDAAPKDAALHAMADALEEREG
                     EILEANARDMEAGAEAGLTDALLDRLSLTPARIAAMARDVRRIAALPDPVGEVIEGRR
                     LPNGLDLRKVRVPLGVVAVVYEGRPNVTIDCSALCLKSGNAIVLRGSSTAEHSNAVLA
                     AVAAEAVAAAGLPDGSVALVAGGGREELAELATRSESVDLIIPRGGEGLKAALSAVAT
                     VPVIYAAAGNCHVYVDATADVQQALAITLNAKLQRPGTCNAAETLLVHGDAAASFLPR
                     AIGDLRAAGVELRVDGRARALAGDGMDALLDASDDDYASEFLALILAVRVVDSVEEAI
                     EHVNRYGSGHSEAIVTSSVEAARAFTEGVDAACVYVNASTRFTDGGEFGLGAEIGNST
                     QKLHARGPIGLRELCTFKYVVEGAGHTRP"
     gene            1282..>1545
                     /locus_tag="VG709_00010"
     CDS             1282..>1545
                     /locus_tag="VG709_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HWC31495.1"
                     /translation="MRIGILGGVFNPPHLGHLVCAQEALIRLELDHVLLVPVGEAPHR
                     PVEADPGAETRLELCRAAVSGDERVGASRVEVDRPGPSYTVDTL"
BASE COUNT          198 a          569 c          575 g          203 t
ORIGIN      
        1 gtccagacgg cctccgtcgg cgagacgtgc gccgaggcga agcgtgccgc acgcacgctg
       61 gcgcgcctag acgccgcgcc caaggacgcg gcgctccacg ccatggccga cgcgctcgag
      121 gagcgggagg gcgagatcct cgaggccaac gcgcgcgaca tggaggccgg ggccgaggcc
      181 ggtctgaccg acgccctgct cgaccggctg tcgctgaccc cggcgcggat cgcagcgatg
      241 gcgcgcgacg tgcgccggat cgcggcgctg cccgacccgg tgggcgaggt gatcgaggga
      301 cggcgccttc ccaacgggct cgatctgcgc aaggtgcgcg tccccctcgg ggtggtcgcc
      361 gtcgtgtacg agggccgtcc gaacgtgacg atcgactgct cggcgctgtg cctcaagtcg
      421 ggcaacgcga tcgttctgcg cgggtcctcg acggccgagc actcgaacgc ggtgctcgcc
      481 gcggtggcgg ccgaggcggt cgctgcggcc ggcctgccgg acggctcggt cgcgctcgtc
      541 gccggcggcg gacgcgagga gctcgccgag cttgcgaccc gctcggagtc ggtcgacctg
      601 atcatcccgc ggggagggga ggggctcaag gccgcgctct ccgcggtcgc caccgtgccc
      661 gtcatctacg ccgccgcggg gaactgtcac gtctacgtcg acgcgacggc cgacgtgcag
      721 caggccctcg ccatcacgct caacgccaag ctccagcgcc ccggcacctg caacgccgcc
      781 gagacgctgc tcgtccacgg cgacgcggcg gcgtccttcc tgccgcgcgc gatcggggac
      841 ctgagggcag cgggggtcga gctgcgcgtc gacggccgcg cccgcgcgct cgccggcgac
      901 ggcatggacg cgctgctcga cgcctcggac gacgactacg ccagcgagtt cctcgccctc
      961 atcctcgccg tccgggtcgt cgactccgtc gaggaggcga tcgagcacgt gaaccggtac
     1021 gggagcgggc actccgaggc gatcgtcacc tcctcggtcg aggcggcgcg cgcgttcacc
     1081 gagggcgtcg atgccgcgtg cgtctacgtc aacgcctcga cccgcttcac cgacggcggc
     1141 gagttcggcc tcggcgcgga gatcgggaac tcgacgcaga agctccacgc gcgcggcccg
     1201 atcggcctgc gcgagctctg cacgttcaag tacgtcgtcg agggcgcggg gcacacgcgg
     1261 ccctagcgga ggcgccggcg cttgcggatc ggcattctcg gcggcgtctt caacccgcct
     1321 cacctcggtc acctggtgtg cgcacaggag gcgctcattc ggctcgagct cgaccacgtc
     1381 ctgctcgtgc cggtcggtga ggcgccgcat cggcccgtcg aggcggaccc tggcgcggag
     1441 acgcgactcg agctctgccg cgcggcggtg tccggcgacg agcgcgtcgg ggcgtcgcgc
     1501 gtagaggtcg accggccggg gccttcgtac accgtcgaca cgctc
//
LOCUS       DASZOB010000002         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131232, whole genome shotgun sequence.
ACCESSION   DASZOB010000002 DASZOB010000000
VERSION     DASZOB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131232"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VG709_00015"
     CDS             complement(<1..863)
                     /locus_tag="VG709_00015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWC31496.1"
                     /translation="MKAEDILDAVGNTPLVGIQRMSANQSVRLWAKLEGQNPTGSLKD
                     RIAKQMIEAAEASGDLTRDRTILEPTSGNTGIALALVARRKGYRLVVVLPSNASEERV
                     RLLELYGAEIVFSAADAGTNGAIDVARRMAEDPAYFMPFQYGNPANPEAHYEGTGAEI
                     VRDLPEITHFVAGLGTGGTLMGVGRRLRAHGADVKIIAAEPELGDLVYGLRSLDDGFV
                     PPIFDDAILDRKFLVSSDDALRATRDLAEREGVFAGLSAGAVLHVARRVTEGLDEGHV
                     VCLLADGGWKYL"
     gene            complement(897..1178)
                     /locus_tag="VG709_00020"
     CDS             complement(897..1178)
                     /locus_tag="VG709_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HWC31497.1"
                     /translation="MAVRLKVPTIFRRHTGGEAVVEGQGQTLKDLLADLDRRHEGFMR
                     AVVTDDGSLHRFVNVYLNDEDVRYLGGLETELRDGDVVSILPAVAGGFR"
     gene            complement(1235..2032)
                     /gene="moeB"
                     /locus_tag="VG709_00025"
                     /pseudo
     CDS             complement(1235..2032)
                     /gene="moeB"
                     /locus_tag="VG709_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(2029..>2218)
                     /locus_tag="VG709_00030"
     CDS             complement(2029..>2218)
                     /locus_tag="VG709_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31498.1"
                     /translation="TEAYPSPTDRAQAHWKDPVTGERVAAYPGTRYLIVSLRDADPVL
                     RAFRFEDGEPVEEEVTIE"
BASE COUNT          330 a          774 c          774 g          340 t
ORIGIN      
        1 aggtacttcc atcccccgtc ggccaggaga cagacgacgt ggccctcgtc gagtccctcg
       61 gtgacgcggc gcgcgacgtg gaggacggct cccgccgaga gcccggcgaa cacgccctcc
      121 cgctcggcga gatctcgcgt cgcgcgcagc gcgtcgtcgg aggacacgag gaacttccgg
      181 tcgaggatgg cgtcgtcgaa gatgggcggc acgaatccat cgtcgagcga ccgaagcccg
      241 tagacgaggt cgccgagctc gggttccgcg gcgatgatct tcacgtccgc gccgtgcgcg
      301 cgcagccggc gtccgacgcc catcagcgtc ccgccggtcc cgagcccggc gacgaagtgc
      361 gtgatctcgg gaaggtcgcg gacgatctcg gcgccggtcc cctcgtagtg cgcctcggga
      421 ttcgccgggt tcccgtactg gaacggcatg aagtatgccg ggtcctcggc catgcgccgc
      481 gcgacgtcga tcgccccgtt cgtgccggcg tccgcggcgc tgaagacgat ctccgcgccg
      541 tagagctcca ggagacggac gcgctcctcg ctcgcattgg agggaagaac cacgacgagg
      601 cggtatccct tccgccgcgc gacgagcgcg agcgcgatcc cggtgttgcc ggaggtcggc
      661 tcgaggatcg tccgatcccg cgtgaggtcg ccggacgcct ccgcggcctc gatcatctgc
      721 ttcgcgatcc ggtccttgag cgagccggtg gggttctgcc cctcgagctt cgcccacagg
      781 cggacggact ggttcgccga catccgctgg atgccgacga gaggcgtgtt cccgaccgcg
      841 tcgaggatgt cctcggcctt caaggtcggg ccatcatgcc acggtcgttc cggccctcag
      901 cggaagccgc cggcgaccgc tgggaggatc gagacgacgt cgccgtcgcg gagctcggtc
      961 tcgagcccgc cgagatagcg cacgtcctcg tcgttcaggt acacgttcac gaatcggtgg
     1021 agcgagccgt cgtcggtcac gacggcgcgc atgaagccct cgtgacggcg atcgagatcg
     1081 gcgaggaggt ccttaagcgt ctgcccctgt ccttccacga cggcctcgcc gccggtgtgg
     1141 cggcggaaga tcgtcgggac cttgagacgg accgccatca gcgctcctgc gccgacggcg
     1201 tgcgctccgc ttcgggcggc gcctgtcccg gtgtgaggcc gcagaaggcc tcgtagtcga
     1261 tcagctccgt gacggtcgga tgcttgccgc acaccgggca gtcgggatcc caccggagct
     1321 tcacggtcgt gaactgcatc gccagcgcgt cgaacagcag gagccgtccg acgagcggct
     1381 cgccgctccc gagcacgagc ttgatcgctt cggtcgcctg gatcgatccg acgatcccgg
     1441 gaaggacgcc gaacacgccg ccctccgcgc acgagggcac cgtgccgggc gggggcggct
     1501 ccgggaacag gcaccgatag cagggcacct cgggacccgg catgaacacc gccgcctggc
     1561 cctcccactg gtagatcgag ccgtagacga gcggcttgcc gagcatctgc gtggcgtcgt
     1621 tgacgaggta ccgcacgggg aagttgtcgg tcccgtcgac gacgacgtcg tagtcgccga
     1681 ggacttcgaa cgcgttttcc gacgagagca gcatctggtg gacccggacg tcgacgttcg
     1741 ggttcaggtc ccggatgcgc tcgcgcgcgg actcgacctt cggccgtccg acgtcacctg
     1801 tgtcgtgcag cagctgccgc tggaggttcg agaggtcgac tcggtcgaag tcgacgaggc
     1861 cgagcgtgcc gaccccggcc gccgcgaggt acatcgcgac cggagagccg agcccgccgg
     1921 cgccgatcac gagcaccttg gagtccatca gcttccgctg cccgtcgccg ccgatgcccg
     1981 ggaggatgat gtgccgcgcg tagcgcagga cctggtcctc ggtgaacgtc actcgatcgt
     2041 cacttcctcc tcgacgggct cgccgtcctc gaaccggaaa gcccgcagca ccggatcggc
     2101 gtcccggagc gacacgatca ggtaccgcgt gcctgggtac gccgcgacgc gttcacccgt
     2161 aacggggtcc ttccagtgcg cctgcgcgcg gtcggtcggc gacgggtagg cctccgtg
//
LOCUS       DASZOB010000003         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_64,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000003 DASZOB010000000
VERSION     DASZOB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VG709_00035"
     CDS             <1..396
                     /locus_tag="VG709_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWC31499.1"
                     /translation="ARLDGVRVVAADVQRPLPFTRRFDVVLAINVVEHLPDHAAGVRT
                     IRDALATGGVCVVHLPTINNVVSRAIYRFAYAGDPTHVYRPSGAEVRSLFESEGFETL
                     EEGFAPFRPKTLWNTLRWHPAYLAAFRRR"
     gene            415..651
                     /locus_tag="VG709_00040"
     CDS             415..651
                     /locus_tag="VG709_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWC31500.1"
                     /translation="MAEILVYGADWCGDCRRSKRLLERRGVEFSWIDVEADPRAAEEA
                     RRVSGRRNIPVIVMPGGEVLVEPSDPELDGRLRA"
     gene            complement(668..1972)
                     /locus_tag="VG709_00045"
     CDS             complement(668..1972)
                     /locus_tag="VG709_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HWC31501.1"
                     /translation="MGANRLQEDLRRGIDLDAVLDHVGDAELVLIGEASHGTDEFYRV
                     RADITKRLVSERGFKAVAVEADWPDAERVNRFVTHPGDDSNGNEALSGFRRFPTWMWR
                     NDVVLEFVEWLREHNAAQGEAPVGFFGLDLYSLNASREAVIRYLERVDREAAERARRR
                     YSCFDPYREDLQDYGLTTLYGEPEPCRDEVVAQLGDLRQRGIEYASRDGRVAQEEFFF
                     AERNAALVRDAEAYYRAMYAGGAESWNLRDRHMAETLESLRRYLERLGREPKVVVWAH
                     NSHLGDARATEMGAAGELNLGQLAREAYGRRVRNVGFTTNAGTVTAASRWGGAAERKR
                     VRPALRGSFEALFHETGVPEFVLLPGSDHALADGLGRRLERAIGVIYRPETERASHYF
                     HARLADQFDVVIHVDETRALRPLEPMSEWDPGEPPETYPWAV"
     gene            complement(2036..2251)
                     /locus_tag="VG709_00050"
     CDS             complement(2036..2251)
                     /locus_tag="VG709_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31502.1"
                     /translation="MQLFRSEEHVERWLEERGLPRGALLTPEQLWGLARTWYENRLEP
                     DWRRRTPDEAEAVFGALGLAGDFWKLT"
     gene            complement(2258..>2538)
                     /gene="merB"
                     /locus_tag="VG709_00055"
     CDS             complement(2258..>2538)
                     /gene="merB"
                     /locus_tag="VG709_00055"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HWC31503.1"
                     /translation="PGTLDVWMANPLSAVPTPFLAEVGGRTWYGNCAWDAPGIVAMLG
                     ANGRVETACPDCGDRLEMTIRGRDLEPIEAVGHFAIPARKWWDDIGFT"
BASE COUNT          356 a          943 c          856 g          383 t
ORIGIN      
        1 gcccggctcg acggcgtgcg ggtcgtggcg gcggacgtcc agcgaccgct ccccttcacg
       61 cggaggttcg acgtcgtgct cgcgatcaac gtcgtggagc accttcccga tcacgcggcc
      121 ggcgtgcgga cgatccgcga cgcgctcgcg acgggcggcg tgtgcgtcgt ccaccttccg
      181 acgatcaaca acgtggtgag ccgcgcgatc taccggttcg cgtacgcggg cgacccgacg
      241 cacgtctacc ggccgtcggg cgccgaggtc cgctcgctgt tcgagtccga gggcttcgag
      301 acgctcgagg aaggcttcgc accgttccgc ccgaagacgc tctggaacac gctccgttgg
      361 catccggcct acctggccgc gttccgccgt cgctgagcgg gtatctaagg agcgatggcc
      421 gagatcctcg tctacggcgc cgactggtgc ggcgattgcc gccggtcgaa gcggctgctc
      481 gagcgtcgcg gcgtcgagtt ctcttggatc gacgtcgagg ccgacccgcg cgccgccgag
      541 gaggcccgac gggtctccgg caggcgcaac atccccgtga tcgtgatgcc gggcggcgag
      601 gtcctggtcg agccgtccga cccggagctc gacgggcggc tccgcgcgta gccggaccgg
      661 ccgccgctca cacggcccac ggatacgtct cgggcggctc cccgggatcc cactcgctca
      721 tcggctccag cggacgaagc gctcgcgtct cgtcgacgtg gatcacgacg tcgaactggt
      781 cggcgaggcg tgcgtggaag taatgactcg cgcgttcggt ctcgggacgg tagatcacgc
      841 cgatcgcccg ctccagacgc cgaccgagcc cgtcggccaa cgcgtggtcc gaccccggaa
      901 gcagcacaaa ctccggcacc ccagtctcgt gaaacagtgc ctcgaagctc ccccggagcg
      961 ccgggcggac ccgcttgcgc tcggcggcgc cgccccacct cgaggcggcc gtcaccgtgc
     1021 cggcgttggt ggtgaagccg acgttccgca cccggcgccc gtacgcctcg cgcgccagtt
     1081 ggccgaggtt cagctcgccg gccgctccca tctcggtcgc gcgcgcgtcg ccgaggtgcg
     1141 agttgtgcgc ccacacgacg accttcggct cgcgcccgag ccgctcgagg taccgacgca
     1201 ggctctcgag cgtctccgcc atgtggcgat cgcggaggtt ccacgactcg gccccgcccg
     1261 cgtacatcgc gcggtagtac gcctcggcgt cccggacgag cgcggcgttg cgctcggcga
     1321 agaagaactc ctcctgcgcg acgcgtccgt cccggctcgc atactcgatc ccccgctgcc
     1381 gtagatcgcc gagctgggca acgacctcgt cgcggcacgg ctctggctcg ccgtacaacg
     1441 tcgtgaggcc gtagtcctgg aggtcctccc ggtacgggtc gaaacacgag taccggcggc
     1501 gtgcgcgctc cgcggcctcg cggtcgaccc gctcgaggta ccggatcacg gcctcgcgcg
     1561 acgcgttcag gctatagagg tcgaggccga agaagccgac cggcgcctcg ccttgcgcgg
     1621 cgttgtgctc acgcagccat tcgacgaact cgaggacgac gtcgttccgc cacatccacg
     1681 tcgggaagcg gcggaagccg ctcagcgcct cgttgccgtt tgagtcgtcg cccgggtgcg
     1741 tcacgaaccg gttgacgcgc tcggcgtcgg gccaatcggc ctcgaccgcg acggccttga
     1801 acccgcgttc actcacgagg cgtttggtga tgtcggcgcg gacgcggtag aactcgtcgg
     1861 tcccgtggga cgcctcgccg atcaggacga gctcagcatc tcccacgtgg tcgagaaccg
     1921 cgtcgaggtc gatcccgcgc cggagatcct cctggagccg gttcgcgccc acggctcgcc
     1981 cctacccggc gccgacgcgc ggaaacacgc gggccgaccg cggctcgcgc gcgacctacg
     2041 tcagcttcca gaagtcgccg gcaagcccga gtgctccgaa tacggcctcc gcctcgtccg
     2101 gcgtgcggcg acgccagtct ggctcgagtc ggttctcgta ccaggtcctc gccaggcccc
     2161 acagctgctc gggcgtgagg agcgcaccgc gcggaagccc tcgctcctcg agccatctct
     2221 cgacgtgctc ctccgaccgg aagagctgca tcgtgcttca ggtgaacccg atgtcgtccc
     2281 accacttccg cgcgggaatc gcgaagtggc cgacggcctc gatcggctcc aggtcacgtc
     2341 cgcggatcgt catctcgagc cggtcgccgc aatccggaca cgccgtctcg acgcgcccgt
     2401 tcgcgccgag catcgctacg atcccgggcg cgtcccatgc gcagttgccg taccacgtgc
     2461 ggccgccgac ctcggcgagg aacggcgtag ggaccgcaga cagcgggttg gccatccaga
     2521 catcgagcgt cccgggct
//
LOCUS       DASZOB010000004         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_30315, whole genome shotgun sequence.
ACCESSION   DASZOB010000004 DASZOB010000000
VERSION     DASZOB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30315"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..273)
                     /locus_tag="VG709_00060"
     CDS             complement(4..273)
                     /locus_tag="VG709_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWC31504.1"
                     /translation="MCRSIQTLYNFEPPAKDEEVRAAALQYVRKISGFTRPSRANAEA
                     FERAVDAVAAASERLLGELVTSAPRKDREAEAAKARARRAQRQAA"
     gene            complement(348..>1534)
                     /locus_tag="VG709_00065"
     CDS             complement(348..>1534)
                     /locus_tag="VG709_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC31505.1"
                     /translation="LRAVDDSDAGREKTPVARWGRFVTSRAKIVFPVVLVVVLALAST
                     SLLVRLGAADQGTQPVEQTSRRAYDLLAEGFGPGFNGPIPIVVDVNGDAEAPQRVYEG
                     VEGLAGVASAGEPQLNDEETVAIIFVTPDSAPQDEETDALVDRLREDVVPAATAGGDA
                     VAYVSGQTAAFKDIADQILERMPLFLLYIIGVTFIVLAMAFRSIVISATAAVTTLLSA
                     FVGFGGLTLVVQEGHLLGITGLDRTGPIETFVPPIAFAILFGLSMDYMVFLMSRIREE
                     HVHGLRTRLAVEHGVAAIGRVVVAAALIMGTVFAAFILSADRIPKEFGLLLAVAILTD
                     ALLVRMTLVPAFLTLLGEKAWYIPRWLDRLLPNLTIEAPHDGEAPRTLASDRPAEAAG
                     TP"
BASE COUNT          233 a          530 c          553 g          218 t
ORIGIN      
        1 aagctaagcg gcctgccgct gcgcccggcg ggcgcgcgcc ttcgccgcct ccgcctcacg
       61 gtccttccgc ggcgccgatg tcacgagctc gccgagaagg cgttcggaag cggctgcgac
      121 cgcgtcgaca gcccgctcga acgcctcggc gttcgcccgc gacggccttg tgaacccgct
      181 gatcttgcgg acgtactgga gggccgcggc gcgtacctcc tcgtccttcg ccggcggctc
      241 gaagttgtag agcgtctgga tgcttcggca catgacgaga gcctagccgc gtcgacgcgt
      301 ccgtacgagg aatgtcacgc gcgtcggtcg tctgcgtcgt cggcggttca cggcgtgccg
      361 gccgcctcgg cgggcctgtc ggaggcgagc gtgcgcggcg cctcgccgtc gtgcggcgcc
      421 tcgatcgtga ggttcggcag cagccggtcg agccagcgcg ggatgtacca ggccttctcg
      481 cccaggaggg tgaggaatgc gggcacgagc gtcattcgca cgagcagcgc atccgtgagg
      541 atcgccaccg cgagcaggag gccgaactcc ttcgggatgc gatcggcgct caggatgaag
      601 gccgcgaaca cggtgcccat gatgagcgct gcggccacga ccacgcggcc gatcgcggcg
      661 acgccgtgct ccacggcgag gcgcgtcctc aggccgtgaa cgtgctcctc ccggatgcgg
      721 ctcatcagga agaccatgta gtccattgag aggccgaaga ggatcgcgaa ggcgatcggt
      781 gggacgaacg tctcgatcgg accggtccgg tcgagcccag tgatgccgag caggtggccc
      841 tcctgcacca ccagcgtgag gccgccgaac ccgacgaacg cggagaggag cgtcgtgacc
      901 gccgccgtcg cggagatgac gatcgagcgg aaggccatcg cgagcacgat gaaggtgacg
      961 ccgatgatgt agaggaggaa gagcggcatc cgctccagga tctggtcggc gatgtccttg
     1021 aacgccgccg tctgccccga cacgtacgcg acggcgtcgc cgcccgccgt cgccgccggg
     1081 acgacgtcct cccgcagccg gtcgacgagc gcgtcggtct cctcgtcctg cggggcggag
     1141 tcgggggtga cgaagatgat tgcgaccgtc tcctcgtcgt tcagctgcgg ctcgcccgcg
     1201 gacgcgacgc ccgcgaggcc ctcgacgccc tcgtagacgc gttggggcgc ttccgcgtcg
     1261 ccgttcacgt ccacgacgat cgggatcggg ccgttgaagc cggggccgaa gccctcggcc
     1321 aggaggtcgt acgccctccg gctcgtctgc tcgacaggct gcgtcccctg gtctgccgcg
     1381 ccgaggcgca cgagcagcga ggtcgacgcg agcgcgagca cgacgaccag gacgacgggg
     1441 aagacgatct tcgcgcgact cgtcacgaag cgaccccagc gggcgacggg ggtcttctcg
     1501 cgtcccgcat ccgagtcgtc caccgcgcgc agga
//
LOCUS       DASZOB010000005         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191883, whole genome shotgun sequence.
ACCESSION   DASZOB010000005 DASZOB010000000
VERSION     DASZOB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191883"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VG709_00070"
     CDS             complement(<1..432)
                     /locus_tag="VG709_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWC31506.1"
                     /translation="MSAGARTTRRVDPGFFLYVTPVLWGATFPAAKLGLEAIGILPFM
                     AWTRLLGFVAILAAIPFVPRREITAPAVRRVLLPGLLLGTLIFVAYILQTVGLSMTKS
                     TNAGFITGLYVVFTPLLGLVLFRRGARASAWVAVVVSLAGMT"
     gene            complement(434..>1771)
                     /locus_tag="VG709_00075"
     CDS             complement(434..>1771)
                     /locus_tag="VG709_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWC31507.1"
                     /translation="HVHLSGTGLEMDGLDLSGARSREETLALAREHASRRSGPALGLG
                     FDETRWPSPQLPAIDELDAVTSEPLVLLRADGYLSVVNTAALKASGIEALDGLERGDR
                     SRPTGLLRGEANAAVQLWYFESLSVEDVAAAQLRACERAASRGVTCVHEMAIPDKRGR
                     RDVEILLLQADDLAVDVVTYIADRDFPYVVGDLLQTRIGGDLFLDGSIGARTAALFEP
                     YADGGGQGSLAYDDDELAEFLHNAHLASLQTGVHVIGDAAIEQALRVWERVYHSLDSR
                     GRRHFRARRHRLEHFEMASIEQIERAAALGLAISIQPGFDAYWGGPGEMYERRLGQDR
                     ARTMNPFRSTLERGLEVGAGSDSPVTPLDPMFGVWALENHHDPAQRMGREDAIRLFTV
                     GAARLAHLQKKGRLEPGASADFAAYGADPFEVPDVRDLRPVLTVSRGRRVHSA"
BASE COUNT          277 a          632 c          637 g          225 t
ORIGIN      
        1 cgtcatcccg gcgagcgaca cgacgaccgc gacccacgcg gaggcgcgcg cgccgcgccg
       61 gaacagcacg agcccaagga gcggggtgaa cacgacgtag aggccggtga tgaaccccgc
      121 gttcgtggac ttcgtcatcg agaggccgac ggtctggagg atgtacgcga cgaagatcag
      181 cgtcccgagg aggagcccag gcagcagcac ccgccgcacg gcaggcgcgg tgatctcgcg
      241 acgaggcacg aacgggatcg cggcgaggat cgcgacgaac cccaggagcc gcgtccacgc
      301 catgaacggc aggatgccga tcgcctcgag tccgagcttc gcggcgggga acgtcgcccc
      361 ccacagcacc ggcgtgacgt agaggaagaa ccctgggtcg acgcgccgcg tcgtgcgcgc
      421 gcccgctgac acgctacgcg gaatgcacgc ggcgtccgcg cgagacggtg aggaccgggc
      481 ggaggtctcg cacgtccggg acctcgaacg ggtcggcgcc gtacgcggcg aagtcggcgg
      541 aagcgcccgg ctcgagacgt cccttcttct gcaggtgcgc gagccgcgcc gccccgaccg
      601 tgaatagccg gatcgcatcc tcgcggccca tgcgctgcgc ggggtcgtga tggttctcga
      661 gcgcccacac gccgaacatc gggtcaagcg gcgtgaccgg cgagtccgag ccggcgccca
      721 cctccagtcc tcgctcgagc gtcgatcgga acgggttcat cgtcctggcg cgatcctgcc
      781 cgagccgccg ctcgtacatc tcgcccggcc caccccagta cgcgtcgaat cccggctgga
      841 tcgagatcgc caggccgagc gccgccgccc gctcgatctg ctcgatcgag gccatctcga
      901 agtgctcgag ccggtgccgc ctcgcgcgga agtgccggcg gccacgcgag tcgagggagt
      961 ggtagacgcg ctcccagacg cgcagcgcct gctcgatcgc cgcatccccg atcacgtgga
     1021 cgccggtctg caggctcgcg aggtgggcgt tgtggaggaa ctccgccagc tcgtcgtcgt
     1081 cgtacgcgag cgagccttgg ccgccgccgt ccgcataggg ctcgaagagc gccgcggtcc
     1141 gcgcgccgat cgagccgtcg aggaacaggt cgccgccgat tcgggtctgg aggaggtcgc
     1201 cgaccacgta ggggaagtcg cggtcggcga tgtacgtcac gacgtcgacg gcgaggtcat
     1261 cggcctggag caggaggatc tcgacgtcgc gacgcccgcg cttgtcgggg atcgccatct
     1321 cgtgcacgca cgtgacgcct cgcgacgcgg cacgctcgca cgcgcgcagc tgcgcggccg
     1381 ctacgtcctc aaccgacagc gactcgaagt accacagctg cacggcggcg ttggcctcac
     1441 cgcggagaag cccggtgggc cggcttcggt cgccgcgctc gaggccgtcg agcgcctcga
     1501 tccccgaggc cttcagcgct gcggtgttga cgaccgacag gtagccgtcg gcccgcagca
     1561 gcacgagggg ctcggatgtc actgcgtcga gctcgtcgat cgcggggagc tgcggagacg
     1621 gccagcgtgt ctcgtcgaag ccgagcccga gcgccggccc ggaccgtcgg ctcgcgtgct
     1681 cgcgcgcgag cgcgagagtc tcctcgcgcg accgggcacc cgagaggtcg agcccgtcca
     1741 tctccagccc ggttcccgac aggtgcacgt g
//
LOCUS       DASZOB010000006         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_60688, whole genome shotgun sequence.
ACCESSION   DASZOB010000006 DASZOB010000000
VERSION     DASZOB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60688"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="rpe"
                     /locus_tag="VG709_00080"
     CDS             <1..729
                     /gene="rpe"
                     /locus_tag="VG709_00080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWC31508.1"
                     /translation="VEHRALRVQPLPLLDAATCLMSGLAGVAPSILSADFARLGEQVK
                     SVEPYADMIHVDVMDAHFVPPLTIGPVVVESLRPVTRLPLHCHLMVERPEGLFDELAD
                     AGSDMVTFHVEAAPDPKVVIRRARDREMRVGVAVNPETDVDAVFAVLEDVDNVIVMTL
                     ERTGWAGQPFKEASLAKIEAVRREIDRRGLAVDVEADGGINVDTGRRCIEAGATVIAA
                     ASSVFKSDDPAEAARRLAEAARGD"
     gene            722..>1608
                     /locus_tag="VG709_00085"
     CDS             722..>1608
                     /locus_tag="VG709_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWC31509.1"
                     /translation="MTDDAPDEQGGPERILVVDDDPDIARFIDVNLRAHGYDVVLASD
                     GVEALEKAREAPPNLALVDVMMPRMDGFELVERLRGGVRTANVPVIILTARALTADKV
                     VGLTAGADDYIIKPFDPVELVARVKGTLRRAQEMRAASPLTGLPGNVQIREHLTRLIA
                     KGQPFALLYADLDNFKAYNDHYGFLRGDVAIRAMSHCIQEAARDVAGDDVFVGHVGGD
                     DFVVACRPHQAEPICETIVSRCSAESPKLYDDADRERGHIEVESRRGGVERFPMLSIS
                     IGAARSDRRPFTHPGEAVEV"
BASE COUNT          251 a          548 c          573 g          236 t
ORIGIN      
        1 gtcgagcacc gagctctacg cgtgcaacca ctgccgctcc tcgatgctgc aacctgtctg
       61 atgagcgggc tcgcgggcgt cgcgccctcg atcctgtcgg ccgacttcgc gcggctcggc
      121 gagcaggtga agtcggtgga gccgtacgcc gacatgatcc atgtggacgt catggacgcg
      181 cacttcgtcc ccccgctcac gatcggaccc gtcgtcgtcg agtcgctacg acccgtcacc
      241 cgcctcccgc tccactgtca cctcatggtc gagcggccgg aaggcctctt cgacgagctc
      301 gccgacgcgg ggagcgacat ggtgacgttc cacgtcgagg ccgcgcccga cccgaaggtg
      361 gtgatccgta gggcgcggga ccgcgagatg cgtgtggggg tcgccgtgaa ccccgagacc
      421 gacgtcgacg cggtgttcgc ggtgctcgag gacgtcgaca acgtcatcgt gatgacgctc
      481 gagcgcacgg gttgggcggg ccagccgttc aaggaggcga gcctcgccaa gatcgaggcg
      541 gtccggcgcg agatcgaccg gcggggtctc gccgtcgacg tggaggccga tggcggcatc
      601 aacgtcgaca ccggccgtcg gtgcatcgaa gccggagcga cggtgatcgc cgctgcgtcg
      661 tcggtgttca agtccgacga tcctgcggag gccgcccgtc ggctcgcgga ggccgcgcgc
      721 ggtgactgac gacgcgccgg acgagcaagg cggacccgag cgcatcctcg tcgtcgacga
      781 cgatcccgac atcgcgcgat tcatcgacgt gaatctccga gcgcatgggt acgacgtcgt
      841 gctcgcctcg gacggcgtcg aggcgctcga gaaggctcga gaggcgccgc cgaacctcgc
      901 gctggtagac gtgatgatgc ctcggatgga tgggttcgag ctcgtcgagc ggctgcgcgg
      961 cggggtccgc acggcgaacg tgccggtaat catcctgacc gccagggcgc tcaccgcgga
     1021 caaggtcgta gggctcacgg ccggcgcgga cgactacatc atcaagccgt tcgaccccgt
     1081 cgagctcgtg gcgcgcgtca aggggacgct gcgccgggcc caggagatgc gcgcggcgtc
     1141 gcccctcacc gggctcccgg gcaacgtcca gatccgcgag cacctcacgc ggctcatcgc
     1201 caaagggcag ccgttcgcgc tgctctacgc ggacctcgac aacttcaagg cctacaacga
     1261 ccactacggg ttcctccgtg gcgacgtggc gattcgggcg atgtcgcact gcatccagga
     1321 agcagcgcgc gacgtcgcgg gcgacgacgt cttcgtcggc cacgtcgggg gagacgactt
     1381 cgtcgtcgcg tgtcgcccgc accaggcgga gccgatctgc gagacgatcg tctcacgctg
     1441 ctcggccgag tcgccgaagc tctacgacga cgcagaccgc gagcgcgggc acatcgaggt
     1501 ggagagccgc cggggcggtg tagagcgctt cccgatgctg tcgatctcga tcggcgccgc
     1561 ccggagcgac cggcggccgt tcacgcaccc gggcgaggcg gtcgaggt
//
LOCUS       DASZOB010000007         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_142,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000007 DASZOB010000000
VERSION     DASZOB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VG709_00090"
     CDS             <1..394
                     /locus_tag="VG709_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWC31510.1"
                     /translation="MAVIADFESYPEWADVKSAKVLARDDDGRATEAEFDVSVPVLGD
                     THYTLEYEYEDDDRGISWTTKEISGSVKDIEGGYALEPLNGDTRVTYWLAVELGMRVP
                     GFLKRQADKRITQTALHALKDRVERNRS"
     gene            438..1634
                     /locus_tag="VG709_00095"
     CDS             438..1634
                     /locus_tag="VG709_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWC31511.1"
                     /translation="MRILLFTGKGGVGKTTVAAATAVRAARSGLRTLVTSTDPAHSLA
                     DSFGVPLGDDVSEVIPKLWAEQIDAQARLEANWRDIQEYVISFLQWMGVESIEAEELS
                     VIPGLDEIFSLTDVRRHVDSGRYDLFVVDCAPTAETLRLLSLPEVMNWYIERIFPIQR
                     GVVKTIRPVLTRVTSMPIADDRIFSAIERLHRNLEAVHKILTDVDRSSVRLVVNPEKM
                     VIAEARRTYTYLSLFGYRVDAVIANRIIPDDVTDPYFGKWKDIQAEHLATIRESFQPA
                     PILQARLFEQELVGVDLLERMGEEVYGDEEPTKVLHRDQPIRVRKRGAWYVLSMRLPL
                     ADKDELDVFRKGDELFVRVGSYKRSLVLPHALQRLDVRQARFTDEGLEIRFAERSGTG
                     ARAAPS"
     gene            1644..1895
                     /locus_tag="VG709_00100"
     CDS             1644..1895
                     /locus_tag="VG709_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31512.1"
                     /translation="MAQSRKRAATTSRTEDGSRHDGVCPVAFCPVGMFLTVSGRARPD
                     AVEHLMAAGREFLLALTAVLNARAEDVGRAPAIEKIELE"
     gene            1910..2959
                     /locus_tag="VG709_00105"
     CDS             1910..2959
                     /locus_tag="VG709_00105"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HWC31513.1"
                     /translation="MSPPDRELLRKLADWPTDGDPVTTLYLEVDGRRHPRRADYVVRA
                     DDLLNKAVERASIDGAAADAVRRDAERIRRFVDEEFERKGVRGVVLFSCAAAGLWDDV
                     RLAQPVRDRAVVRESPYLLPLEATLELAERICVAVVDRAKARLFITNLAEIEEVSEVL
                     DDVPARHDQGGWSQARHQRHIEDHVQRHLRRVADLLLRLRQRRRYEHLILSGPDGVTS
                     ELERGLHEYVRRVVADRAAFPVSTPPATVLEHALEVERRIDADREAAAVRTLSEALAS
                     PSGRAVAGMAPTLEALEAGRVEMLVVLATPSADVDDRVEDAVEAALRRGSRVESVAGS
                     GQLERLGGIGALLRF"
BASE COUNT          467 a         1037 c         1085 g          454 t
ORIGIN      
        1 catggcggtg atcgcagact tcgagtcgta tccggagtgg gccgacgtca agtcggcgaa
       61 ggtcctcgcg cgggacgacg acgggcgtgc gaccgaggcg gaattcgacg tctccgtccc
      121 ggtcctcggc gacacccatt acacgctcga gtacgagtac gaggacgacg accgcggcat
      181 ctcgtggacg accaaggaga tctcgggctc ggtcaaggac atcgagggcg gctacgccct
      241 cgagccgctc aacggcgaca ccagggtgac gtactggctc gccgtcgaac tcggtatgcg
      301 cgtgccgggt ttcctcaagc gccaggccga caaaaggatc acgcagacgg cccttcatgc
      361 gctgaaggac cgggtagagc gcaaccgctc gtagcgagga ctgcggcgag cggccgacgg
      421 ccgctacgat ggcgcccatg cggatcctgc tgttcaccgg caagggaggc gtggggaaga
      481 ccacggtcgc cgccgctacg gccgtccgcg ccgcccggtc cggccttcgc acgctcgtga
      541 ccagcaccga cccggcacac tcgctggctg actccttcgg cgtcccgctc ggcgacgacg
      601 tcagcgaggt gatcccgaag ctctgggcgg agcagatcga cgcgcaggcc cgcctcgagg
      661 cgaactggcg cgacatccag gagtacgtga tctcgttcct gcagtggatg ggcgtcgagt
      721 cgatcgaggc ggaggagctg tcggtgatcc cggggctcga cgagatcttc agcctcaccg
      781 acgtgcgccg ccacgtcgac agcggccgct acgatctctt cgtcgtcgac tgcgccccaa
      841 cggcggagac gctccggctg ctctcgctcc cggaggtcat gaactggtac atcgagcgga
      901 tcttcccgat ccagcgcggc gtcgtgaaga cgatccgccc cgtgctgacg cgcgtcacgt
      961 cgatgccgat cgcggacgac cggatcttct cggcgatcga gcggctccac cgcaacctcg
     1021 aggcggtgca caagatcctc accgacgtcg accgctcgtc ggttcgcctc gtcgtcaacc
     1081 cggagaagat ggtgatcgcg gaggcgcgcc ggacgtacac gtacctgtcg ctgttcgggt
     1141 accgcgtcga cgcggtgatc gccaaccgca tcatccccga cgacgtgacc gatccgtact
     1201 tcgggaagtg gaaggacatc caggcagagc acctcgctac gatccgtgag tcgttccagc
     1261 ccgccccgat ccttcaggcg cgcctgttcg agcaggagct cgtcggcgtc gatctcctcg
     1321 agaggatggg ggaggaggtc tacggcgacg aggagccgac gaaggtcctg caccgcgacc
     1381 agccgatccg ggtgcgcaag cgcggcgcat ggtacgtcct gtcgatgcgt cttcccctcg
     1441 ccgacaagga tgagctcgac gtcttcagga agggcgacga gcttttcgtc cgcgtcggct
     1501 cgtacaagag gagcctggtg ctgccgcatg cgctgcagcg actcgacgtg cggcaggcgc
     1561 gtttcaccga tgagggactc gagatcaggt tcgccgagcg ctctgggacg ggcgctcgcg
     1621 cggccccgag ctaggaggaa ccgatggcgc aatcccgcaa gcgagcggcg acgacgtcac
     1681 gtaccgagga cggatcacgc cacgacggcg tctgcccggt cgcgttctgt ccggttggga
     1741 tgttcctgac ggtgagcggg cgggcgcgtc ccgacgccgt cgagcacctc atggcggcgg
     1801 gccgggagtt cctgctcgcg ctcacagccg tcctcaacgc tcgcgcggag gacgtcggcc
     1861 gcgccccggc gatcgagaag atcgagctcg agtagccggg aggcccaacg tgtcgcctcc
     1921 cgatcgcgag ctgctgcgga agctggccga ctggccgacc gacggcgacc ccgtgacgac
     1981 gctgtacctc gaggtcgacg ggcgtcgtca tccccgccgc gcggactacg tcgtccgcgc
     2041 cgacgacctg ctgaacaagg ccgtagagcg ggcgtcgatc gacggcgccg ccgccgacgc
     2101 cgtccggcgc gacgccgagc ggatccgccg gttcgtcgac gaggagttcg agcggaaggg
     2161 cgtccgcggc gtcgtgctgt tctcgtgcgc cgcggcaggg ctgtgggacg acgtcaggct
     2221 ggcgcagccg gtgcgcgacc gtgccgtcgt gcgggagtct ccgtaccttc ttccgctcga
     2281 ggcgaccctc gagctcgccg agcggatctg cgtcgcggtc gtcgatcgtg cgaaggcgcg
     2341 cctgttcatc acgaacctcg ccgagatcga ggaggtgtcg gaggtcctcg acgacgtccc
     2401 ggcacggcac gatcagggcg ggtggtcaca ggcgcgtcac cagcggcaca tcgaggacca
     2461 cgtccagcgg catctcagac gcgtcgcgga ccttctgttg cggctccgcc agcgccgacg
     2521 ctacgaacac ctgatcctgt ccggacccga cggcgtgacg tccgagctcg agcgcgggct
     2581 ccacgaatac gttcgccgcg tcgtcgccga ccgcgccgcg ttcccggtct ccacgccgcc
     2641 ggcgacggtg ctcgagcatg cgctcgaggt cgagcgacgg atcgacgccg accgcgaggc
     2701 ggcggccgtg cgcacgctct cggaggcgct cgcgtccccg tcggggcgcg cggtggccgg
     2761 gatggcaccg acgctcgagg cgctcgaggc cggccgcgtc gagatgctcg tcgtactcgc
     2821 gaccccgagc gccgacgtcg acgatcgcgt cgaggacgcc gtcgaggcgg cgctccgccg
     2881 cggctcccgc gtggagagcg tcgcgggttc ggggcagctc gaacggctcg gcgggatcgg
     2941 cgcgctgctg cggttctgat cgtggtccgg cggctggcga tgggcatcga cgtcgggggc
     3001 accaaggtcg caggtgtcgt cgtcggcgac gacggtgagg tgg
//
LOCUS       DASZOB010000008         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_70899, whole genome shotgun sequence.
ACCESSION   DASZOB010000008 DASZOB010000000
VERSION     DASZOB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70899"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VG709_00110"
     CDS             <1..901
                     /locus_tag="VG709_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC31514.1"
                     /translation="FAREEAGLLVRPAAEVPGPATRAVERSLRERGASFLSEVAADAG
                     LPEDEALRALFELVWVGRVTNDTLGGIREPERARGRRRARGRHPLYGRWSMVPPPVED
                     AAERATAWAARLLASYGVVAREMAAAAEVPTPWPLVADALATLEARGEVRRGYFVRGL
                     SGVQYAATGAVDRLRRRSSSGGLRLVAASDPANAYGPLLPLPAERPYRLARVPGNWLV
                     VRDGVPLLAVEGRGRRLVPLSDGVEDALPMLAELARASSRGRMAVERWDADPVVASEG
                     AELLARAGFVRGPRQMTYRAPVA"
     gene            complement(880..>1801)
                     /locus_tag="VG709_00115"
     CDS             complement(880..>1801)
                     /locus_tag="VG709_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HWC31515.1"
                     /translation="SELLRISPPVASALDAGRPVVALETSVLAQGLMPPHNAEAADAI
                     ERAIRELGSEPAWTWVGDGAVRIGAARDELPALMAPGHATKVSRRDGPLAVAGGALGA
                     TTVSAAVWAAHRAGIPIGATGGTGGVHPGENDVSADLLELARTAVTLVCSGPKTILDP
                     VATAERLEELGIAVVGYRCDRLPFFVVREAPVDLEHRLDTARAVADAARARDALEIRS
                     ALLLCNPVPERHALAAEEMRDAVERCRAQAQSAGITGKALTPYLLTCLAERTDGASVE
                     ANLALLESNARVAAEIAVELSGAAQATGAR"
BASE COUNT          197 a          675 c          689 g          240 t
ORIGIN      
        1 gttcgcgcgc gaggaggcgg ggctcctcgt ccggccggcc gcggaggtgc ccgggccggc
       61 gacgcgcgcg gtcgagcgct ccctccggga gcgaggggcg tcgttcctct cagaggtggc
      121 cgcggacgcc ggactccccg aggacgaggc actgcgtgcg ctcttcgagc tggtgtgggt
      181 cgggcgcgtt acgaacgaca cgctcggcgg catccgcgag ccggaacgcg cgcgaggcag
      241 gcgtcgagcg cgcgggcggc acccgctcta cgggcgctgg tcgatggttc cccccccggt
      301 cgaggatgcc gcggaacgag cgacggcgtg ggccgcgcgg ctcctcgcgt cctacggcgt
      361 cgtcgcgcgc gagatggccg cagcggccga ggtcccgacg ccgtggccac tcgtcgcgga
      421 cgcgctcgcg acgctcgagg cgcgtgggga ggtgcgccgc ggctacttcg tccgcggcct
      481 ctccggtgtt cagtacgcgg cgaccggcgc ggtcgatcgg cttcggcgcc gttcatcgtc
      541 cggcggcctt cgtctcgtcg ccgcctccga ccccgcgaac gcgtacgggc cgctgctgcc
      601 ccttcccgcc gaacggccgt accgcctcgc ccgcgtgccc gggaactggc tcgtcgtccg
      661 ggacggcgtg ccgctgctcg cggtcgaggg ccgcggccgt cgtctcgtgc cgctctccga
      721 cggcgtcgag gacgcgcttc cgatgctcgc ggagctcgcg cgcgcctcgt cgcgcggacg
      781 gatggcggtc gagcgatggg acgccgaccc cgtcgttgcg tccgaagggg ccgagctcct
      841 cgcgcgggct gggttcgttc gcgggccgcg ccagatgacc tatcgcgcgc ccgtcgcctg
      901 agcagcgccg gacagctcga ccgcgatctc ggccgcgacg cgggcgttgg actcgagcag
      961 cgcgaggttc gcctcgacgc tcgcgccgtc ggtccgctcg gccaagcagg tgagcaggta
     1021 aggcgtgagc gccttgccgg tgatccccgc ggactgcgcc tgcgcgcgac accgctcgac
     1081 ggcgtcgcgc atctcctccg cagcgagcgc gtgacgctcg gggaccgggt tgcagaggag
     1141 caacgcggac cggatctcga gcgcgtcccg tgcgcgagcg gcgtccgcga cggcccgcgc
     1201 cgtgtcgagc cggtgctcga ggtcgaccgg agcctcgcgt acgacgaaga acggtaggcg
     1261 gtcgcaccgg tagccgacga ccgcgatccc gagttcctcg agccgctcgg ccgtggccac
     1321 ggggtcgagg atcgtcttcg gtcccgagca cacgagcgtg acggctgtgc gcgcgagctc
     1381 gaggaggtcc gctgaaacgt cgttctcgcc cgggtggacg ccgccggtgc cgccggtggc
     1441 gccgatcggg atccccgcgc ggtgtgccgc ccacacggcg gccgacacgg tggtggcgcc
     1501 gagcgccccg ccggcgacgg cgagcggccc gtcgcgccgt gagaccttgg tcgcatggcc
     1561 cggcgccatg agcgccggca gctcgtcccg agctgcaccg atgcggaccg ctccatcgcc
     1621 gacccacgtc catgccggct ccgagccgag ctcgcggatc gcgcgctcga tcgcgtcggc
     1681 cgcctcggcg ttgtgcggcg gcatcagtcc ctgcgccagc acgctggtct cgagcgccac
     1741 caccggccgc cccgcgtcga gcgccgacgc gacgggcggc gagatccgga ggagctcgct
     1801 c
//
LOCUS       DASZOB010000009         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_60839, whole genome shotgun sequence.
ACCESSION   DASZOB010000009 DASZOB010000000
VERSION     DASZOB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60839"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VG709_00120"
     CDS             <1..919
                     /locus_tag="VG709_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31516.1"
                     /translation="IVDSPRGVARGTVTVQAHVTGGVGDAEYAAYRVGGNWIQMEKAG
                     EGRFTSSSRPWGTAGVANGDYTVEVRVWGDVPAYEANDSNTYASQAVTVSVDNPPPAP
                     AGVSARPGGGGVEVSWRPVATADRGDFIGYRVLGGRAPSCANAPYRQLAETSGTVFTH
                     TAAAYGTWCYRVVALRASNVSGAVASAPSAPASVALRAARVPSTSIPEGFGGVGAADG
                     RDAAPPAPPALGDGKLKVSDGRFDEDLPYGARRVTQEVEGRVPGALRGASDEPGVGPR
                     QGLTLVAAGLVLATFALLLRRFLAAAPEP"
     gene            916..1167
                     /locus_tag="VG709_00125"
     CDS             916..1167
                     /locus_tag="VG709_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31517.1"
                     /translation="MSDRDAVACRRCGETLVEALTPDEVVTRGGRRVRFRRHTDFVMC
                     DRCFSLYRIGDLRAGRVEPLSDDDLVSAGEATPEEDQPS"
     gene            1332..>1860
                     /locus_tag="VG709_00130"
     CDS             1332..>1860
                     /locus_tag="VG709_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HWC31518.1"
                     /translation="MNTSSRRPSRRSIAVTAAIALVVGMLWPAAAAAHPDATGHQKGT
                     EHDLGLGVRVNSPDNLAGFIPAVLWSGTPLPDGETSDLAYAGTGCTPAHYAAADVEGK
                     IAIVDDINGSLFPGDRCAYAFVQKVQSAQAAGAIGLIQVERNDEIFEGNAVTGDIPAL
                     AVAKSDGAPVRDAVIA"
BASE COUNT          256 a          655 c          704 g          245 t
ORIGIN      
        1 gatcgtcgac agcccccgcg gggtcgcgcg cggaaccgtc accgtgcagg cgcacgtcac
       61 cggaggggtc ggcgacgccg agtacgccgc gtaccgggtc ggcggcaact ggatccagat
      121 ggagaaggcg ggcgaggggc ggttcacttc gtcgtcgcgg ccgtggggga cggcgggcgt
      181 cgcgaacggc gactacacgg tcgaggtccg ggtttggggc gacgtgccgg cgtacgaggc
      241 gaacgactcg aacacgtacg cgtcgcaggc cgtcacggtg agcgtcgaca acccgccgcc
      301 ggctcccgca ggcgtatccg cacggccggg aggcggaggc gtcgaggtct cgtggcggcc
      361 cgtcgcgacg gcggaccgcg gcgacttcat cgggtaccgc gtcctcggcg gccgcgctcc
      421 gtcctgcgcg aacgcgccgt accgtcagct cgcggagacc agcggcacgg tgttcacgca
      481 cacggcggcg gcgtacggca cgtggtgcta ccgcgtcgtc gcgctgcgtg cgagcaacgt
      541 ctcgggggcg gtcgcgtcgg cgccatcggc gccggcctcc gtggcgctca gggcggctcg
      601 cgtcccgtcg acgagcatcc cggagggctt cggcggtgtc ggggcggccg acggccgcga
      661 tgccgcgcca ccggcgccac cggcgctggg cgacggaaag ctgaaggtgt ccgacggacg
      721 gttcgacgag gatctcccgt acggcgcacg acgcgtcacc caggaggtcg aaggccgggt
      781 gcccggcgct ctccgcggcg cgtccgacga gcccggcgtc gggccccggc aggggctcac
      841 gctggtcgcg gccgggctcg tgctcgcgac gttcgcgctg ctgctgcgac ggttcctcgc
      901 cgcggcgccc gagccgtgag cgaccgagac gccgtcgcgt gccgtcgctg cggcgagacg
      961 ctcgtcgagg cgctcacccc cgacgaggtc gtgacgcgag gcgggcggcg ggtccgcttc
     1021 cgccggcaca ccgacttcgt gatgtgtgac cgctgcttct cgctctaccg catcggcgac
     1081 ctgcgcgccg ggcgcgtgga gccgctatcg gacgacgacc tggtgtccgc tggcgaggcg
     1141 acgccggaag aggatcagcc gtcgtaggag ctgcaccaca ggcgcgcttt gtgcggtagc
     1201 gcacacttcc gcgcagacga ggcaggaatg cgccgcgtcc gcgtctaaat cgccacccac
     1261 cacgtgccgg aaaccgttga acgctgcccg ctgcctcctc gacgcggtcg cgggcggggg
     1321 gaggactgga gatgaacacg agtagcaggc ggccgtcccg ccgttccatc gccgtgaccg
     1381 cggcgatcgc gctggtcgtc gggatgctgt ggccggcagc ggccgcggcg caccccgacg
     1441 cgaccggcca tcagaagggc accgagcacg acctcgggct cggggtccgg gtgaactccc
     1501 cggacaacct ggccggcttc atccccgctg tgctgtggag cggcactcct ctgcctgatg
     1561 gcgagacgtc ggacctggcg tacgcaggaa ccggctgcac tcccgcccac tacgcggccg
     1621 ccgacgtcga ggggaagatc gcgatcgtcg acgacatcaa cggaagcctg ttcccgggcg
     1681 acaggtgcgc gtacgccttc gtccagaagg tgcagtctgc ccaggcggca ggcgccatcg
     1741 ggctcatcca ggtcgagcgc aacgacgaga tcttcgaggg gaatgccgtc accggggaca
     1801 tcccggcgct cgcggtcgcg aagtcggacg gcgctccggt ccgtgacgcc gtcatcgccg
//
LOCUS       DASZOB010000010         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10217, whole genome shotgun sequence.
ACCESSION   DASZOB010000010 DASZOB010000000
VERSION     DASZOB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10217"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..372)
                     /locus_tag="VG709_00135"
     CDS             complement(7..372)
                     /locus_tag="VG709_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31519.1"
                     /translation="MGDASGDAAVVREAHERLARGDLEGFAELVADDATWVGVTPEDG
                     AEPATCANRQGIVETLRQNLQASLHGELARIVQGTDGLDGRVGDWFHSIETRAGRIAR
                     MRDAVTWAEALVAAGASDG"
     gene            867..>1769
                     /locus_tag="VG709_00140"
     CDS             867..>1769
                     /locus_tag="VG709_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWC31520.1"
                     /translation="MAIAKVMGIETEYGISGPGNADFNPVLSSSLLINTFAGTLRRIR
                     WDYEQESPLRDARGFEPVQEREPSEEDLGLANVILPNGARYYVDHAHPEYSTPECATP
                     RDLVVHDKAGERILETSLQLVAELLPPGERVLVYKNNTDGKGNSYGCHENYLVDRQAP
                     FGRIVRDLTPFFVTRQIFTGAGKVGVEGSHPEAEHVTYQLSQRPDFFEAEVGLETTLK
                     RPIINTRDEPHADPEKYRRLHVIVGDANMCEVATFLKVGTTALVLQMIEDEALPDLSL
                     ESPVGALHEVSWDPTLTTTIRLADG"
BASE COUNT          264 a          630 c          606 g          269 t
ORIGIN      
        1 gtacctctag ccgtccgacg ctccggcggc gacgagcgcc tcggcccacg tgacggcgtc
       61 gcgcatgcgc gcgatcctgc cggctctcgt ctcgatggag tggaaccagt cgccgacgcg
      121 cccgtcgagg ccgtcggtcc cctggacgat gcgcgcgagc tcgccgtgga gcgacgcctg
      181 gaggttctgc cggagggtct cgacgatccc ctggcggttc gcgcacgtcg cgggctcggc
      241 gccgtcctcc ggggtgacgc cgacccacgt cgcgtcgtcg gcgacgagct cggcgaaccc
      301 ctcgagatcg ccgcgggcga gccgctcgtg cgcctcccgc acgaccgccg cgtcgccgct
      361 cgcatcgccc acacctcaac cgtacgctag gagcgcgatg tcggctcgcg acggatccct
      421 cgagaccttc ggcggtctcg tccggagatc gtcgcccggc gatcgagtcg accggcttcg
      481 tgtggaacga gagcgggctc gtcggcgatc ggagggccgg cgacgtcacg gcgttgttcg
      541 agacggctcc ccgtgagctg ggccacgcct gcccggacct gttcgggtag ttcggcgacg
      601 acgacgtctg cgtcgacctg tggatcggct acgggccgac ctcgcgacca tggcggccga
      661 cctcgaggga ccggacgtcg cctcgatgct ccgcgagtac ggtcgcgggg gcgctgcgga
      721 cgctcgtagc gtcgcttcgc acgtccggcg acctccgagg acagctgtcc gcgctcgccg
      781 acggcctcac gacattgctg ttcgacgtcg gaccgggtcg cgcttaggac ccctccccgg
      841 tcgggggccg tatcctagga agcctcatgg cgatcgcaaa ggtgatgggc atcgagaccg
      901 agtacgggat ctcggggccc ggcaacgccg acttcaaccc ggtgctgtct tcgtcgctgc
      961 tgatcaacac gttcgccggg acgctccggc ggatccgttg ggactacgag caggagagcc
     1021 cgctccgcga cgcgcggggc ttcgagccgg tgcaggagcg cgagccgagc gaggaggatc
     1081 tcgggctcgc caacgtgatc cttcccaacg gcgcgcgtta ctacgtcgac catgcccacc
     1141 ccgagtactc gacgccggag tgcgcgacgc ctcgcgacct cgtcgtgcac gacaaggcgg
     1201 gggagcggat cctcgagacc tcgctgcagc tcgtcgcgga gctgctgccg ccgggggagc
     1261 gcgtgctcgt ctacaagaac aacaccgacg gcaagggcaa ctcgtacggg tgtcacgaga
     1321 actacctcgt cgaccgccag gcgcccttcg ggcggatcgt tcgcgatctc acaccgttct
     1381 tcgtgacccg ccagatcttc accggcgccg ggaaggtcgg cgtcgaggga tcgcatcccg
     1441 aggccgagca cgtcacgtac cagctgagcc agcggcccga cttcttcgag gccgaggtcg
     1501 gcctcgagac gacgctgaag cggccgatca tcaatacgcg cgacgagcca cacgccgacc
     1561 ccgagaagta ccgccgcctc cacgtgatcg tcggggacgc caacatgtgc gaggtcgcga
     1621 cgttcctcaa ggtcgggacg accgcgctcg tcctacagat gatcgaggac gaggcgctgc
     1681 ccgatctgtc tctcgagtct ccggtcggtg cgctgcacga ggtctcgtgg gacccgacgc
     1741 tcacgacgac catccgtctc gccgacggg
//
LOCUS       DASZOB010000011         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10218, whole genome shotgun sequence.
ACCESSION   DASZOB010000011 DASZOB010000000
VERSION     DASZOB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10218"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VG709_00145"
     CDS             <1..561
                     /locus_tag="VG709_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWC31521.1"
                     /translation="TRELLDFTRMTGAVVQRMRELPVPIVAAVNGVAAGAGAVIALAA
                     DVRVLARSARFAFLFTKVGLAGADMGSAYLLPRLIGLGRATELLLLGEDLDAERAEAI
                     GLANRVVDDEVLSDEAEALARRLAQGPALAHATTKMLLSREQDMDLAGSLELEAMAQA
                     LLMTSDDHREFYAAFTQRRDPSWRGR"
     gene            558..965
                     /locus_tag="VG709_00150"
     CDS             558..965
                     /locus_tag="VG709_00150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWC31522.1"
                     /translation="MSETPHEIVNPPSLPRPSGFSHALVAASGRLVFLGGQAGHRADG
                     SLVGPGLVEQFEQAASNVADALRAAGGAPEHLVQLLIFVTDISAYRDALKDVGEAYRR
                     HFGRHFPPMALFGVTELFDPGAKVELVGVAVLP"
     gene            complement(969..1175)
                     /locus_tag="VG709_00155"
     CDS             complement(969..1175)
                     /locus_tag="VG709_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HWC31523.1"
                     /translation="MATNVQVYNDPAADAWSVKVTGDERSETQRFASKDEAVEAGQRL
                     AEERGVELVVHDQRGLQEEKRSEG"
     gene            complement(1202..1873)
                     /locus_tag="VG709_00160"
     CDS             complement(1202..1873)
                     /locus_tag="VG709_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWC31524.1"
                     /translation="MSARGTSPVERDVGRRGLNRLEAFSDGVFAIAITLLVLQFDVPP
                     LDTPDTELAEELLGQFPQFLSFALSFLVIGRYWVAHHSTFAYIRRYDGTFLFLNLVLL
                     FGVAFLPYPTEVLGEHGDQGTAVVLYAASISAVSVASSAIWWYATRRHRLVDPDLDPA
                     FIRSVQLRAASGAAVFLVSIPLAFVSVYASYAVWAVFFPLVRVGIRRRERRRTLRSDE
                     PGREG"
     gene            complement(1870..3021)
                     /locus_tag="VG709_00165"
     CDS             complement(1870..3021)
                     /locus_tag="VG709_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC31525.1"
                     /translation="MSGFGLTEAQQQLAEKARRIGREVLAPIARDGLEGRVNRRLVKA
                     LADRGLLPRLFPGRAGGAREGEISAVELCVVREALARESTEAETALAMQGLGGYPILQ
                     SGTEATIRRWVPPIARGDAVAAFALTEPGHGSDAAAIELRADRDGDGYRLSGVKTWIS
                     NAPDADVYVVFARTTPATGARGVTAFVVPGDAEGVSGAPIELLAAHPIGRLELDTVRV
                     AVDQVLGKPNEGFRVAMRTLDLFRPSVGAFAVGMAQAALDASLSHAEIREVFGKPLRE
                     LQSIAHLLAEMATRTEAARLLVYAAASAYDGGERVTRRSAMAKLFATETAQWVVDSAL
                     QVHGASGLERGHLLEHLYREVRAPRIYEGTSEIQREIIARELFRERGDA"
     gene            3073..4434
                     /locus_tag="VG709_00170"
     CDS             3073..4434
                     /locus_tag="VG709_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC31526.1"
                     /translation="MRIESPYWNPRHETLPREDLEALQFRKLRSLLEWSDANVPFYAK
                     RMRDAGVTPDAVRTLDDLRRLPFLTRDEWMQGQLESPPYGTLLAQPPERAIRYHMTSG
                     TTGRTPIRVLDSMKDWEWISEMWCYGLWGFGVRPADTMFVAFGYATFIGFWGLHYAGE
                     KIGCLVMPGGAMQTDARVKQIVDMGATVVASTPTYALRMAQEARALGIDLASSSVDKL
                     ILSGEPAGSIPATKNLIEEQWGAKAGDTAGMTELGTIMIFECDRQPGGTHIIEDHYIE
                     EVVDPETDEPVAYGELGERVVTSFGRAFLPVVRYRTRDFVVRVPASECSCGRSFDVYQ
                     GGIRGRVDDMKLVRGTNVYPRAVEAIIREFKEIDEFQIHLYTDEGIRDEIEVLVEIPD
                     EGADAASILDRLSRELAETHEGLRFGVKQVENGTLPRFELKAKRLVDDRIVIGTEGER
                     RQP"
     gene            4431..4625
                     /locus_tag="VG709_00175"
     CDS             4431..4625
                     /locus_tag="VG709_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31527.1"
                     /translation="MTDDLNEGERMLVEAYRTLERALGERRSDLAPFAERNALKALAA
                     LWQVMNGLDQAPGQPYDLGA"
     gene            complement(4650..4808)
                     /locus_tag="VG709_00180"
     CDS             complement(4650..4808)
                     /locus_tag="VG709_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31528.1"
                     /translation="MADEGPRGYGSKWRENWWKYLLVYLVIGGIAYAIIYYVFLADGG
                     GGGGGYGG"
     gene            complement(4878..>5603)
                     /locus_tag="VG709_00185"
     CDS             complement(4878..>5603)
                     /locus_tag="VG709_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HWC31529.1"
                     /translation="ERWRAASDAAHDRGAVFAIEIGHAGRRGATRPRSEGLDRPLAAP
                     WELIAPSPLAYGRRGPAPREMTEDDMQRVGGDFAAAAKRAALARADLLLVHMGHGYLI
                     ASFLSPLTNRRSDRFGGSLEARMTFPLEVLRSVREGWSDERPLGVVLTATDWARGGLD
                     VEDAVAVARTLREHGCLLVLPVAGQTIRETRPRYGPAYLSPYAERIRTEARVATIASG
                     RITTSGRANTIVGGGRADLVVIS"
BASE COUNT          827 a         1967 c         2014 g          795 t
ORIGIN      
        1 acgcgcgagc tcctcgactt cacgcggatg accggcgcgg tcgtccagcg gatgcgcgag
       61 ctcccggtcc cgatcgtcgc tgcggtgaac ggagtggcgg cgggcgccgg agcggtgatc
      121 gcgctggcgg ccgacgtgcg ggtgctcgcg cgctcggcac ggttcgcctt cctgttcacg
      181 aaggtcggtc tggcgggcgc cgacatgggt tcggcgtatc tcctgccgcg cttgatcggg
      241 ctgggacgcg cgaccgagct gctcctcctg ggcgaggacc tggacgcgga acgagccgag
      301 gcgatcgggc tcgcgaaccg cgtcgtcgac gacgaggtgc tgtccgacga ggcggaagcg
      361 ctcgcgcggc gcctcgcgca aggtcctgcg ctggcccacg caacgaccaa gatgctgctc
      421 tcccgcgagc aggacatgga cctcgccggc tcgctcgagc tcgaggcgat ggcgcaggcg
      481 ctgctcatga ccagcgacga ccaccgcgag ttctacgctg cgttcacgca gcgacgcgat
      541 ccgagctggc gaggccggtg agcgagaccc cgcacgagat cgtcaacccg ccctcgcttc
      601 cgcggccgag cgggttctcc cacgcgctcg ttgcggcgag cgggcggctc gtgttcctcg
      661 gcggccaggc cggccaccgc gccgacggat cgctcgtcgg tccggggctc gtcgagcagt
      721 tcgagcaggc ggcgtcgaac gtggcggatg cgcttcgcgc ggccggcggc gcgcccgagc
      781 acctcgtgca gctgctgatc ttcgtgaccg acatctccgc gtaccgcgac gccttgaagg
      841 atgtcggcga ggcgtaccgc cgtcacttcg ggcgtcactt cccgccgatg gcgctgttcg
      901 gcgtgacgga gctcttcgac cccggcgcga aggtcgagct ggtcggcgtc gcggtcctgc
      961 cctgacgctc agccctcgga gcgcttctcc tcctggagcc cgcgctggtc gtggacgacg
     1021 agctcgacgc cgcgctcctc ggcgaggcgt tgccccgcct cgaccgcctc gtccttggac
     1081 gcgaaccgct gcgtctcgga cctctcgtcg ccggtcacct tgacgctcca cgcgtcggcc
     1141 gcgggatcgt tgtagacctg cacgttcgtg gccatgtccc tcctctcgtc tcggcgtctg
     1201 gttacccttc gcggcccggc tcgtcactcc ggagcgttcg tcggcgctcg cggcgcctga
     1261 tcccgacgcg gacgagcggg aagaacaccg cccagacggc gtaggacgcg tacacgctca
     1321 cgaacgcgag cgggatcgag accaggaaga ccgcggcacc tgacgctgcg cggagctgga
     1381 cgctccggat gaacgccgga tcgaggtcgg ggtcgacgag ccggtgacgc ctggtcgcgt
     1441 accaccagat cgccgacgac gcgacgctga ccgccgagat gctcgcggcg tagaggacaa
     1501 cggccgtgcc ctggtccccg tgctcgccga gcacctccgt cgggtacggg aggaacgcga
     1561 cgccgaacag caacacgagg ttgaggaaca ggaacgtccc gtcgtagcgc cggatgtacg
     1621 cgaacgtcga gtgatgggcg acccagtacc ggccgatgac caagaacgag agcgcgaagc
     1681 tcaggaactg cgggaactgc cccaagagtt cctctgcgag ctccgtgtcg ggagtgtcca
     1741 gcggcggaac gtcgaactgc agcacgagca gcgtgatcgc gatcgcgaag acgccgtcgc
     1801 tgaaggcctc gagccggttg aggccgcggc gcccgacgtc gcgctcgacc gggctcgttc
     1861 cgcgcgcgct cacgcgtcgc cgcgctcgcg gaagagctcg cgagcgatga tctcgcgctg
     1921 gatctccgac gtcccctcgt agatccgggg cgcgcggacc tcccggtaga ggtgctcgag
     1981 gagatgcccg cgttcgagcc cggaggcgcc gtggacctgg agcgcggagt cgacgaccca
     2041 ctgcgccgtc tcggtcgcga acagcttcgc catcgcggag cgccgtgtta cccgctcgcc
     2101 cccgtcgtac gccgacgccg cggcgtacac gagcagccgc gcggcttccg tgcgcgtcgc
     2161 catctcggcg aggaggtgcg cgatcgactg gagctcgcgc agcggcttcc cgaacacctc
     2221 gcggatctcg gcatgggaca gcgaggcatc gagcgcggcc tgcgccatcc cgaccgcgaa
     2281 cgcgccgacg ctcgggcgga agaggtcgag ggtccgcatc gctacgcgga acccctcgtt
     2341 cggcttcccg agcacctggt cgacggcgac gcggacggtg tcgagctcga gccgcccgat
     2401 cgggtgggcg gcgagcagct cgatcggtgc tccggagacc ccttcagcat cgcccggaac
     2461 gacgaacgcc gtgacgcccc gcgccccggt cgcgggcgtg gttcgcgcga agaccacgta
     2521 cacgtcggcg tccggcgcgt tcgagatcca ggtcttcacg ccgctcagcc ggtacccgtc
     2581 gccgtcccgg tcggcgcgca gctcgatcgc ggcggcgtcc gacccgtgcc ccggctcggt
     2641 gagcgcgaac gcggccaccg cgtcgccgcg tgcgatcggc gggacccagc gccggatcgt
     2701 cgcctcggtt ccggactgca ggatcgggta gccgccgagt ccctgcatcg cgagcgccgt
     2761 ctcggcctcg gtcgactcgc gcgcgagcgc ctcgcggacg acgcacagct cgacggccga
     2821 gatctccccc tcccgagctc cgccggcgcg tcccgggaag aggcggggga ggagcccgcg
     2881 gtccgcgagc gccttcacga gccggcggtt cacgcgaccc tccagcccgt cgcgggcgat
     2941 cggtgcgagg acctcgcggc cgatacggcg cgccttctcg gcgagctgct gctgcgcctc
     3001 ggtgagcccg aagccgctca tcgcgctgag ggtaggatgc tcgacgggtc acgcgcgagg
     3061 cggaggaggg cgatgaggat cgagtcgccg tactggaacc cgcggcacga gacgctgccg
     3121 cgcgaggatc tcgaggcgct ccagttccgg aagcttcgga gcctccttga gtggtccgac
     3181 gcgaacgtcc ccttctacgc gaaacggatg cgcgacgccg gcgtgacgcc ggatgccgtc
     3241 cgcacgctcg acgacctgcg tcgtctgccg ttcctcacgc gtgacgagtg gatgcagggc
     3301 cagctcgaga gcccgccgta cgggacgctg ctcgcgcagc cgcccgagcg cgcgatccgc
     3361 taccacatga cctcgggaac gaccggtcgc accccgatcc gggtgctcga ctcgatgaag
     3421 gactgggagt ggatctccga gatgtggtgc tacgggctct gggggttcgg cgtgcggccg
     3481 gccgacacga tgttcgtcgc gttcgggtac gcgacgttca tcgggttctg ggggctccac
     3541 tacgccgggg agaagatcgg gtgcctcgtc atgcccggag gtgccatgca gaccgatgcg
     3601 cgcgtgaagc agatcgtcga catgggagcg acggtcgtcg cgtcgacgcc gacgtacgcg
     3661 ctccggatgg cacaggaggc gcgagcgctc gggatcgacc tcgcgtcgtc gtcggtcgac
     3721 aagctgatcc tctccgggga gcccgccggg tcgatcccgg ccacgaagaa cctgatcgag
     3781 gagcagtggg gcgcgaaggc cggcgacacg gcggggatga ccgagctcgg gacgatcatg
     3841 atcttcgagt gcgaccgcca gccaggaggg acgcacatca tcgaggacca ctacatcgag
     3901 gaggtcgtcg accccgagac cgacgagccg gtcgcgtacg gcgagctcgg ggaacgcgtc
     3961 gtcacatcgt tcgggcgcgc gttcctcccg gtggtccggt accggacccg agacttcgtt
     4021 gtccgcgtgc cggcgagcga gtgctcatgt ggtcggtcgt tcgacgtcta ccagggcggg
     4081 atccgaggtc gcgtcgacga catgaagctc gtgcgcggga ccaacgtcta cccgcgcgcc
     4141 gtcgaggcca tcatccgcga gttcaaggag atcgacgagt tccagatcca cctctacacc
     4201 gacgagggga tccgcgacga gatcgaggtc ctcgtggaga tccccgatga gggtgcggac
     4261 gctgcgtcga tcctcgaccg cctcagcagg gagctcgcag agacgcacga ggggctgcga
     4321 ttcggcgtga agcaggtgga gaacggcacg ctgccgcggt tcgagctgaa ggcgaagcgg
     4381 ctcgtcgacg accggatcgt gatcggaacc gaaggagagc ggaggcagcc gtgacggacg
     4441 acctcaacga gggcgagcgg atgctcgtcg aggcctatcg gacgctcgag cgcgcgctcg
     4501 gcgagcggcg cagcgacctg gcgccgttcg cggagcgcaa cgctctcaag gcgctcgcgg
     4561 cgctctggca ggtgatgaac gggctcgacc aggcccccgg tcagccgtac gacctcggcg
     4621 cctaggtcgc ggagcggccg cggagaccgc tatccgccgt agccgccgcc gcctccgccg
     4681 ccgtcggcga ggaacacgta gtagatgatc gcgtacgcga tgccgccgat gaccagatac
     4741 accagcaggt acttccacca gttctcgcgc cacttgctgc cgtagcccct cggtccctcg
     4801 tccgccacgg ttcctcccgt agtcgtcgtc gcgccgagca tatgaacgtc gttccgccgg
     4861 agcaacgcca gaccgcgtca gctgatgacg acgaggtccg cgcggccgcc gccgacgatc
     4921 gtgttcgcgc gtcctgatgt cgtgatacgc cccgacgcga tcgtcgcgac gcgcgcctcg
     4981 gttcggatgc gttccgcgta cggcgagagg taggcggggc cgtaccgcgg ccgggtttcg
     5041 cgaatcgtct gtccggcgac gggcagcacg aggagacacc cgtgctcgcg gagcgtcctc
     5101 gcgacggcga ctgcgtcctc gacgtccagc ccgccgcgcg cccagtcggt cgcggtgagg
     5161 acgacgccga gcggccgttc gtcggaccag ccctcgcgca ccgaccggag cacctccagc
     5221 ggaaacgtca tccgcgcctc gagggagccg ccgaaccgat cgctccgccg gttcgtcagg
     5281 ggcgagagga acgaggcgat gaggtacccg tgccccatgt ggacgaggag gagatcggcg
     5341 cgcgcgagcg cggcgcgttt cgccgcggcg gcgaagtcgc cgccgacgcg ctgcatgtcg
     5401 tcctcggtca tctcgcgcgg ggcggggccc cggcgaccgt acgcgagagg cgacggcgcg
     5461 atcagctccc agggggcggc gaggggccgg tccagcccct cgctgcgagg ccgtgtcgcg
     5521 cctcgccgac ccgcgtgccc tatctcgatc gcgaagacgg cgccccggtc gtgcgcggcg
     5581 tccgacgcgg cacgccaccg ctc
//
LOCUS       DASZOB010000012         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_342,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000012 DASZOB010000000
VERSION     DASZOB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VG709_00190"
     CDS             <1..973
                     /locus_tag="VG709_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC31530.1"
                     /translation="LDLWWFNQSWPARKFEEGQEVAAFGKVGARGGRLQMQNPAVEVL
                     RGSGADQVHTRRIIPVHPVGEGVSTGVVRRLAYDALRRLPELEDPLPEDVRARHDLPP
                     FPEALHDIHFPEHPIAEQRARWRLKFDELFFLELGVAFRKHRIEAETEGIAHDLSGDL
                     AERFVASLPFDVTGAQRRAMDEVAKDMAAAKPMNRLLQGDVGSGKTVVALHACLVAIG
                     SGHQAAIMAPTEVLAGQHLRTVASLLDAIGARRLDAASTSPQASLLDAPAAKGPSYAL
                     LTAAVTGKDRQTIVEAAASGGLALLIGTHALVQEHVELADLGVAVID"
     gene            1043..1873
                     /locus_tag="VG709_00195"
     CDS             1043..1873
                     /locus_tag="VG709_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWC31531.1"
                     /translation="MTATPIPRTLALTYYGDLDVSVLDEMPAGRKPVATRIARGASER
                     RRAYDLVREQVENGRQAFVVTAAIDEANKIEVRAAEQEAERLATDVFPDLRIVLLHGR
                     MRPKEKEERMERFRSGEADVLISTTVIEVGVDVPNATVMLVENAERFGLAQLHQLRGR
                     IGRAEHQSFCVLFDESSPENEDARARLEAMVATTDGFELADTDLMLRGEGTLFDVKQS
                     GLPDLRLARLAEDHELVKQARADAFAAIDADPDLSAHPELKRELERRFADSIDWLFHS
                     "
     gene            complement(1870..>2592)
                     /locus_tag="VG709_00200"
     CDS             complement(1870..>2592)
                     /locus_tag="VG709_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31532.1"
                     /translation="TWFGTEPLDWGSLFERWAPAQLIHGESTRTLSAGGIMAHEVARK
                     VTVSVTGSDAAALYLVHDSGTGVTVQPIAGHTVDIGNVKRGARVYAVVVRPVAGSERT
                     TLSVTAKKPPAASPAQAPVTGSVVTLAPGVGVRVGGITSQYVQFEVPEDVDNARAEVR
                     ATNTLPGDVDLYMQQRNAAGTWTAVPGAEGESSSTTGESMDVGRLEAGTYRIEVHNWA
                     GPPGNQVNVRTTFFNSAGEAGA"
BASE COUNT          377 a          913 c          927 g          375 t
ORIGIN      
        1 cctcgatctc tggtggttca accagtcgtg gccggcgcgc aagttcgagg aggggcagga
       61 ggtggcggcg ttcgggaagg tcggggcgcg cggcggacgc ttgcagatgc agaacccggc
      121 ggtcgaggtc ctgagggggt cgggtgccga ccaggtccac acgcgccgga tcatccccgt
      181 gcaccccgtc ggcgagggcg tctcgacggg cgtcgtccgg cgcctcgcgt acgacgcgtt
      241 gcggcggctc cccgagctcg aggatcccct ccccgaggac gtgcgcgcgc gccacgacct
      301 tccgccgttt ccggaagcgc tgcacgacat ccatttcccg gagcacccga tcgcggagca
      361 gcgggcgcgc tggcggctga agttcgacga gctgttcttc ctcgagctcg gcgtcgcgtt
      421 ccggaagcac cgcatcgagg ccgaaaccga ggggatcgcg cacgacctct ccggggacct
      481 cgccgagcgc ttcgtcgcgt cgcttccgtt cgacgtgacc ggggcgcagc gacgcgcgat
      541 ggacgaggtc gcgaaggaca tggcggccgc gaagccgatg aaccgcctgc tccagggaga
      601 cgtcggttcg gggaagaccg tcgtcgcgct ccacgcgtgc ctcgtcgcga tcgggtcggg
      661 gcaccaggcc gcgatcatgg cgccgaccga ggtgctcgcc ggacagcacc tgcggaccgt
      721 cgcgagcctg ctcgacgcga tcggcgcgcg gcggctcgac gcggcgtcga cctcaccgca
      781 ggcgtcgttg ctcgacgcgc cggcggccaa gggtccgtcg tacgcgctgc tcacggccgc
      841 ggtcaccggc aaggaccggc agacgatcgt cgaggccgcc gcctcgggtg gcctcgccct
      901 cctgatcggg acccacgcgc tcgtgcagga gcacgtcgag ctcgccgacc taggcgtcgc
      961 ggtgatcgac tagcagcacc gcttcggtct tcaccagcgc gtcgcgcaga aggagaaggg
     1021 aacgtccccc gacgtcctga tcatgaccgc gacgccgatc ccgcggaccc tcgcgctgac
     1081 gtactacggc gacctggacg tctcggtgct cgacgagatg ccggcggggc gcaagcccgt
     1141 cgcgacgcgc atcgcgcgcg gcgcctcgga acggcgccgc gcgtacgacc tcgtgcgcga
     1201 gcaggtggag aacggaaggc aggcgttcgt cgtcacggcc gcgatcgacg aggcgaacaa
     1261 gatcgaggtt cgcgcggccg agcaggaagc cgagcgcctc gcgacagacg tatttccgga
     1321 cctccggatc gtgctgctgc acggccggat gcggccgaag gagaaggagg agcggatgga
     1381 gcgattccgc tcgggcgagg ccgacgtgct gatctcgacg acggtgatcg aggtcggcgt
     1441 cgacgtcccg aacgcgaccg tgatgctcgt cgagaacgcc gagcggttcg ggctcgccca
     1501 gctgcaccag ctccgcggcc ggatcggtcg ggccgagcac cagtcgttct gcgtgctgtt
     1561 cgacgagtcc tccccggaga acgaggacgc gcgtgcaagg ctcgaggcga tggtggcgac
     1621 gaccgatggc ttcgagctcg ccgacaccga cctcatgctg cgcggcgagg ggacgctctt
     1681 cgacgtgaag caatcgggcc tgcccgacct tcggctggca cggctcgccg aggaccacga
     1741 gctcgtgaag caggcccggg ccgacgcctt cgcggcgatc gacgccgacc cggacctgtc
     1801 agctcatccg gagctcaagc gtgagctcga gcgccgtttc gccgactcga tcgactggct
     1861 cttccactcc taggcgccgg cctcgcccgc cgagttgaaa aaggtcgtgc ggacgttcac
     1921 ctggttgccc ggcgggccgg cccagttgtg cacctctatg cggtacgtgc cagcctcgag
     1981 ccgaccgacg tccatcgact caccggtcgt cgagctcgac tctccctcgg cgcccggaac
     2041 cgccgtccac gttccggcgg cgttccgctg ctgcatgtag aggtcgacgt cgcccggcag
     2101 cgtgttggtc gctcgcacct cggctcgagc gttgtcgacg tcctccggca cctcgaactg
     2161 cacgtactgg ctggtgatgc cgccgacgcg gacgccgacg cccggcgcga gcgtgacgac
     2221 cgaacccgta accggcgcct gcgcggggct cgcggctggc ggcttcttcg cggtcacgct
     2281 caacgtcgtg cgctcgctgc ccgcgaccgg ccggacgacg acggcgtaga cgcgggcgcc
     2341 gcgcttcacg ttgccgatgt cgacggtgtg gccggcgatc ggctgcacgg tgacgccggt
     2401 tccgctgtcg tgcacgaggt acagggccgc ggcgtcggac ccggtgacgg acacggttac
     2461 cttccgcgca acctcgtgcg ccatgatccc gccggcggag agcgtgcggg tggactcgcc
     2521 gtggatcagc tgcgccggcg cccaccgctc gaacagcgag ccccagtcca gtggctccgt
     2581 accgaaccag gt
//
LOCUS       DASZOB010000013         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_71022, whole genome shotgun sequence.
ACCESSION   DASZOB010000013 DASZOB010000000
VERSION     DASZOB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71022"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VG709_00205"
     CDS             <1..399
                     /locus_tag="VG709_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31533.1"
                     /translation="ASLCKLLELLAPVDRPLSELVDEVPRPVVLHRELRCPFARKGTV
                     MRVVTERMKGRRLDTLDGIKVFADDGWAQVLPDPAEPVVHVFSEGKTEKDARRLEDEF
                     SALVEAIIGNGADEVNVRAGRVGPEPSSEG"
     gene            415..717
                     /locus_tag="VG709_00210"
     CDS             415..717
                     /locus_tag="VG709_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31534.1"
                     /translation="MVYFQTVVDALPEEPVEPLPDLKSLSDADLRDLIDRLVAEEHQV
                     SYQRRLLHGRIDILKAELQARLQKQVGEEGESPLYEVDVDKLAAILASRAAPPNPE"
     gene            767..1225
                     /locus_tag="VG709_00215"
     CDS             767..1225
                     /locus_tag="VG709_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWC31535.1"
                     /translation="MHCPECGFQNPESSNYCSKCGALLIRDEPGNHTTMTFAPEDLVD
                     EEGTALEDVPIEGTALVVRAGGGRQGETFPLEQDRIEVGRSPNADVFLDDVTVSRSHA
                     ILSRDEGGYVIEDNGSLNGTYVNRRRVEKATLDDGDEVQIGKYRLTYLSH"
     gene            1230..1955
                     /locus_tag="VG709_00220"
     CDS             1230..1955
                     /locus_tag="VG709_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWC31536.1"
                     /translation="MATTAQESKERRLTIGAVRDRLADEFPDISISKIRYLEGQGLLV
                     PRRTRSGYRLYSDADVERLRTILRLQRDEFLPLRVIRQELAAGRGEQDQLAKGDWTPD
                     ARALRRASVDVRSGGSLYRLEEVLDDTRADAALVKELEEYGVIRGEKREGETLYDDTE
                     REIVRAVTELKRFGVGGRNLRVFRTSADREAALLEQILAPALRSRNPERRKEAVQALE
                     NLAATASYLKHLLLVRDLRKIAR"
BASE COUNT          335 a          707 c          700 g          290 t
ORIGIN      
        1 gcgagcctct gcaagctgct cgagctgctc gcgccggtcg accggccgct ctcggagctc
       61 gtcgacgagg ttccgaggcc ggtcgtgctc catcgtgagc tccgctgccc gttcgcccgg
      121 aagggcacgg tcatgcgggt cgtcaccgag cgcatgaagg gccggcggct cgacaccctg
      181 gacgggatca aggtcttcgc cgacgacgga tgggcgcagg tcctcccgga ccccgccgag
      241 ccggtcgtcc acgtcttctc ggaggggaag accgagaagg acgcgcgccg cctcgaggac
      301 gagttcagcg ccctcgtcga ggccatcatc gggaacggcg cggacgaagt caatgtccgg
      361 gcgggccgtg tcggccccga accctcaagt gaaggttgaa gccttgccgc gtacatggtt
      421 tacttccaga cggtcgtcga tgctcttccg gaggagcccg tggaacccct tcccgatctg
      481 aaatcgctct cggacgccga ccttcgcgac ctgatcgacc gcctggttgc cgaggagcac
      541 caggtctcgt accagcgccg gctcctgcac gggaggatcg acatcctgaa ggccgagctg
      601 caggcgcggc tgcagaagca ggtcggagag gagggcgaga gcccgctcta cgaggtcgac
      661 gtcgacaagc tcgccgcgat cctggcctcg cgcgccgcgc ccccgaaccc cgagtaggag
      721 gtgcggtgag ccggttccgc gttcaccccc cgagcgcatg acccacgtgc actgtcccga
      781 gtgcgggttt cagaatccgg agagctcgaa ttactgctcg aagtgcggcg cgctgctgat
      841 ccgcgacgag ccgggcaatc acacgacgat gacgttcgcc cccgaggacc tcgtcgacga
      901 ggagggcacg gcgctcgagg acgttccgat cgagggcacc gcgctcgtcg tccgggccgg
      961 gggcggtcgc cagggagaga cgttcccgct cgagcaggac cggatcgagg tcggtcgctc
     1021 gccgaacgcg gacgtcttcc tcgacgacgt gacggtctcg cgctcgcacg cgatcctgtc
     1081 gcgcgacgag ggcgggtacg tcatcgagga caacggcagc ctcaacggga cctacgtgaa
     1141 ccgtcgacgc gtcgagaagg cgacgctgga cgacggcgac gaagtgcaga tcggcaagta
     1201 ccggctcacc tacctctcgc actgagcgca tggcgacgac tgcgcaggag agcaaggaac
     1261 gccgcctcac gatcggggcc gtgcgtgacc ggctcgccga cgagtttcct gacatctcca
     1321 tctccaagat ccgctacctg gagggccagg ggctgctcgt tccgcggcgc acgcgcagcg
     1381 gctaccgcct ctacagcgac gccgacgtcg agcgcctgcg gacgatcctc cgccttcagc
     1441 gcgacgagtt cctgccgctg cgggtgatcc gccaggagct cgcggcggga cgtggcgagc
     1501 aggaccagct ggccaagggt gactggaccc ccgacgcccg tgcgctgcgt cgcgcgagcg
     1561 tcgatgtgcg ctcgggtggc tccctctacc gcctcgaaga ggtgctcgac gacacgcgcg
     1621 cggacgccgc gctcgtcaag gagctcgagg agtacggcgt catccgcggc gagaaacgcg
     1681 agggcgagac gctctacgac gacaccgagc gcgagattgt gcgcgcggtg acggagctca
     1741 agcgcttcgg cgtcggcggg cgcaacctgc gcgtctttcg gacctcggcc gaccgcgagg
     1801 cggcgctgct cgagcagatc ctcgcccctg cactgcgctc gcgcaacccg gagcggcgca
     1861 aggaggcggt acaggccctc gagaacctgg ccgccacggc gtcctacctc aagcacctgc
     1921 tgctggtccg cgacctgcgc aagatcgccc ggtgagcgag accgccgcgg aggctctgcg
     1981 cccccacgtc cgtgacatcc ccgacttccc caagccgggg atcgtcttca ag
//
LOCUS       DASZOB010000014         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_81059, whole genome shotgun sequence.
ACCESSION   DASZOB010000014 DASZOB010000000
VERSION     DASZOB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81059"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..203
                     /gene="rpmC"
                     /locus_tag="VG709_00225"
     CDS             15..203
                     /gene="rpmC"
                     /locus_tag="VG709_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWC31537.1"
                     /translation="MLIEQVRDARQEVFNLRFQLATGQLENTARLREAKKDLARGLTV
                     ARQRHIDVDRELARQRRG"
     gene            379..612
                     /gene="rpsQ"
                     /locus_tag="VG709_00230"
     CDS             379..612
                     /gene="rpsQ"
                     /locus_tag="VG709_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006695831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWC31538.1"
                     /translation="MRQGVVVSDKAPKTLVVRIDTTRQHRVYKKTVRESTTLHAHDER
                     DEARAGDTVRVVESRPLSATKRWRLTEILERAR"
     gene            619..987
                     /gene="rplN"
                     /locus_tag="VG709_00235"
     CDS             619..987
                     /gene="rplN"
                     /locus_tag="VG709_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWC31539.1"
                     /translation="MIQPETRLRVADNTGAREILCIRVRGGSRRRYAGVGDIIVGTVK
                     QAIPHGSVRKGDVVTAVVVRTRKELGREDGTYIAFDENAAVIIDAQQNPRGTRIFGPV
                     ARELRERNFMKIVSLAPEVL"
     gene            1005..1325
                     /gene="rplX"
                     /locus_tag="VG709_00240"
     CDS             1005..1325
                     /gene="rplX"
                     /locus_tag="VG709_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003728539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWC31540.1"
                     /translation="MRRDDTVKVISGKDSGKTGRVLRVEPKRERLFVEGMNMQKRHQR
                     PRSLRDLQKAQQVGGIVESEGPIHISNVKLLDPSTEEPTRVGITREGGVRRRVAKRSG
                     EVID"
     gene            1325..1879
                     /gene="rplE"
                     /locus_tag="VG709_00245"
                     /pseudo
     CDS             1325..1879
                     /gene="rplE"
                     /locus_tag="VG709_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
BASE COUNT          352 a          751 c          731 g          252 t
ORIGIN      
        1 gcctcgacga cggcttgctg atcgagcagg tccgcgacgc tcgccaggag gtcttcaacc
       61 tccgcttcca gctcgcgacg ggtcagctcg agaacaccgc tcgtctccgc gaggccaaga
      121 aggacctggc gcgcgggctg accgtggccc gtcagcgcca catcgacgtc gatcgcgagc
      181 tcgcgaggca gcgccgtggc tgacgggggg gcgagaacgc cggccgaccg agaggcggag
      241 cgcacggagc gtcgccggcg caacgcccgc cagcgccgcg cgtggcgcgc ccgccggcgc
      301 tcgcggcgcg cggagcgcga accgcgaggc ccggcggtca ccgatccggt cgccaggggc
      361 gggcgcgggc ggccgcgcgt gcgccagggc gtcgtggtct ccgacaaggc gccgaagacc
      421 ctcgtcgtac gcatcgacac gacccgccag caccgtgtct acaagaagac cgtccgcgag
      481 tcgacgaccc tgcacgccca cgacgagcgc gacgaggccc gcgccgggga cacggtccgc
      541 gtcgtcgaga gccgtccgct gagcgccacc aagcgctggc gcctgaccga gatcctggag
      601 cgggcgaggt agcacccaat gatccaaccc gagactcgcc tccgggtagc cgacaacacc
      661 ggcgcccgcg agatcctctg catccgcgtg cgcggtggct cgcgccgacg ctatgccggc
      721 gtcggcgaca tcatcgtcgg cacggtcaag caggccatcc cgcacggctc ggtgcgcaag
      781 ggcgacgtcg tcaccgccgt ggtggtacga acccgcaagg agctcggccg tgaggacggg
      841 acctacatag ccttcgacga gaacgccgcg gtgatcatcg acgcccagca gaacccgcgc
      901 gggacgcgca tcttcggacc ggtggcgcgg gagctgcgcg agcgcaactt catgaagatc
      961 gtctccctcg ctccggaggt gttgtagtgg cccacaaggc tcgaatccgt cgcgacgaca
     1021 ccgtaaaggt gatctccggc aaggacagcg gcaagaccgg ccgcgtcctg cgcgtcgagc
     1081 ccaagcgcga gcgcctgttc gtcgagggca tgaacatgca gaagcgccac cagcgccccc
     1141 gcagcctgcg cgacctccag aaggcccagc aggtgggcgg gatcgtcgag tccgaagggc
     1201 cgatccacat ctcgaacgtc aagctcctcg acccgagcac cgaggagccc acgcgcgtgg
     1261 ggattacgcg ggagggcggc gtccgccggc gggtggccaa gcggtcgggc gaggtgatcg
     1321 actgatggcg gtcaagaccc cggcggtcgt cccgcgcctg aggatccggt acgaggagga
     1381 gatccgtccc caactcgtcg agcgctacgg cctgcgcagc gtgatggagg cgccgaggct
     1441 cgagaagatc acgctgaaca tgggggtcgg cgacgccaag caggactcgc gcgtgctcga
     1501 gcgggccggc gagcagctcg ccatcatcgc cggccaggct ccgagcgagc gccgcgcccg
     1561 ccggtcgatc gccggcttca agatccggga cgggatgccg atcggcgtcg ccgtcaccct
     1621 gcgcaacgcg cgcaggtacg agttcctcga ccgcctcacg gcgaccgccc tgccgcgggt
     1681 gcgcgacttc cgcgggctca acccccgggc gttcgacgga cggggcaact actcgctcgg
     1741 gatccgcgag cagatcatct ttccggagat cgactacgac gcgatcgacc gggtgcgcgg
     1801 cctcgacgtg gcgatcacga cctccaccga cagcgacgcg caggcgttct tcctgctgcg
     1861 cgagctgggc atgccgttcc ggcgcgaggg cggacccgac gatcgccctc tgcgcggctc
     1921 cgaggacgag cggggcggac cgagtcagcc cgacgccatc tctccggcca agcatccccc
     1981 cgatgccgag gccgttccct ccggggtcgg ccaggaatcc agctagcgta ggtacgacat
     2041 ggccaagacc tcccagttcg tcaagtccca gcggcagcac aagtac
//
LOCUS       DASZOB010000015         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_60987, whole genome shotgun sequence.
ACCESSION   DASZOB010000015 DASZOB010000000
VERSION     DASZOB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60987"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..453)
                     /locus_tag="VG709_00250"
     CDS             complement(16..453)
                     /locus_tag="VG709_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWC31541.1"
                     /translation="MSLDELYKEVILDHYKAPRNKGPLEGATVSKRKNNPLCGDDIEV
                     HAVLDDGRVAEISFEGQGCSISQASASMLTEAVRGKRLEDTDALAGDFRGMMEGRHEP
                     DEDEFGDLLALKGVVKYPVRIKCAVLAWDVLQDALRDADASPR"
     gene            complement(454..1674)
                     /locus_tag="VG709_00255"
     CDS             complement(454..1674)
                     /locus_tag="VG709_00255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWC31542.1"
                     /translation="MVDFDALRDDFPILRREVHGKRLVYLDSAATSQKPRQVIDALTE
                     FWERHNANVHRGVYLLGQEATALYEGAREKVAALVGAPSSESIVFARGTTEAINLVAH
                     GWGRKFLTEGDEVVLTELEHHSNIVPWQLAARDTGAQLRFVGITGDGRLDPEAVASVI
                     TERTKLVAVSGMSNVLGTIVPVAEIVDIAHEAGAKVLVDGAQLVSHAPVDVVALDVDF
                     LTFSGHKMLGPTGSGGLYARPELLDAMDPFLGGGEMIEEVLPERSTYKSPPWKFEAGT
                     PAIGEAVGLGAAVDYLQAVGMKAIRDHERELTAYALERLDEAGARIFGPRDPDGRGGA
                     VSFWFRDVHPHDLATVVDQEGVAIRAGHHCAQILMRRLGVPATARASFYLYNTTDDVD
                     RLVEALKRAEEVFA"
BASE COUNT          243 a          614 c          572 g          261 t
ORIGIN      
        1 aggatggtag gcacgtcagc gcggcgacgc gtccgcgtcg cggagggcgt cttggagcac
       61 gtcccacgcg agcacggcgc atttgatgcg cacggggtac ttcacgactc ccttgagcgc
      121 aaggaggtcc ccgaactcgt cctcgtcggg ctcgtggcgc ccctccatca tcccgcggaa
      181 gtcgcccgcg agcgcgtcgg tgtcctcgag cctcttccca cgcaccgcct cggtcagcat
      241 cgaggcggac gcctgcgaga tcgagcagcc ctggccctcg aacgagatct cggcgacgcg
      301 gccgtcgtcg aggacggcgt gaacctcgat gtcgtcgccg cagagcgggt tgttcttccg
      361 cttcgagacc gtcgcaccct cgagcggccc cttgttccgc ggcgccttgt agtggtcgag
      421 gatgacctcc ttgtagagct cgtcgagcga catctacgcg aagacctcct ccgccctctt
      481 cagcgcttcc acgagccggt cgacatcatc ggtcgtgttg tagaggtaga acgacgcgcg
      541 cgcggtcgcc gggacgccga gccgtcgcat caggatctgc gcgcagtggt gaccagcccg
      601 gatcgcgacg ccctcctggt ccacgaccgt cgcgaggtcg tgcgggtgca cgtcgcggaa
      661 ccagaacgac accgcaccgc cgcgcccgtc ggggtcccgc ggaccgaaga tccgggcgcc
      721 cgcctcgtcg agccgctcga gcgcgtacgc ggtgagctcg cgctcgtggt cgcggatggc
      781 cttcatcccg accgcctgca ggtagtcgac cgcggccccg agcccgacgg cctcgccgat
      841 cgccggcgtg ccggcctcga acttccacgg cggcgacttg taggtcgagc gctccggcag
      901 gacctcctcg atcatctcgc ccccgccgag gaagggatcc atcgcgtcga ggagctcggg
      961 gcgcgcatac agcccgcccg agcccgtcgg gccgagcatc ttgtggcccg agaacgtgag
     1021 gaagtcgacg tcgagcgcga cgacgtcgac cggcgcgtgc gacaccagct gagcgccgtc
     1081 gacgaggacc ttggcgccgg cctcgtgcgc gatgtcgacg atctcggcga ccgggacgat
     1141 cgtgccgagc acgttcgaca tcccgctgac agcgacgagc ttcgtgcgct cggtgatgac
     1201 cgacgccacg gcctccggat cgaggcggcc gtcacccgtg atcccgacga agcggagctg
     1261 cgcgccggtg tcgcgggccg cgagctgcca cgggacgatg ttcgagtggt gctcgagctc
     1321 cgtgagcacg acctcgtcgc cctcggtgag gaacttccgc ccccagccgt gcgccacgag
     1381 gttgatcgcc tcggtcgtcc cgcgcgcgaa cacgatcgac tccgacgacg gcgcgccgac
     1441 gagcgccgcg accttctcgc gcgctccctc gtagagcgcg gtcgcctcct gcccgagaag
     1501 gtacacgccg cggtggacgt tcgcgttgtg gcgctcccag aactcggtca gcgcgtcgat
     1561 cacctgtcgc ggcttctgcg acgtcgcggc gctgtcgagg tacacgaggc gcttcccgtg
     1621 cacctcacgg cgcaggatgg ggaagtcgtc gcgcagcgcg tcgaagtcga ccacgtccgc
     1681 tcctcgctct
//
LOCUS       DASZOB010000016         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_101357, whole genome shotgun sequence.
ACCESSION   DASZOB010000016 DASZOB010000000
VERSION     DASZOB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101357"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VG709_00260"
     CDS             complement(<1..991)
                     /locus_tag="VG709_00260"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWC31543.1"
                     /translation="MALTILEGSTFCVSDERGDIEGPTDGLFASDTRFLSRWILTLNG
                     ARPHVLSFDKVEYFSAAYYLRNPVAGGLDRDELSIARERFVGEGMQDHVVVQNHAHRR
                     LDFELALEVGTDFADILTVKEHDFALGDPDHANPLPERVTIADADADGHLVLVDPSPF
                     AGVTQLFFSQRGKVDGSTVRYPIALEPGESWGLRVEVTVASDGSRTEPENAERQFGDE
                     LARVRASLSAWHMRIPQLRATWEPLAAAFDRSVSDLASLRIRSAQPEGQLFAAGMPWF
                     MAVFGRDTLITCLQTFLFGPELARNALAVLAELQATEDDPEPDAEPGRSSTRSG"
     gene            complement(997..>1559)
                     /locus_tag="VG709_00265"
     CDS             complement(997..>1559)
                     /locus_tag="VG709_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC31544.1"
                     /translation="FDGVDILVNNAGLALGRTAIADGSDDDDAAMWETNVLGLVRMTR
                     LVVPHMEDRRGHVVNLGSWAGREAYTYGGMYVASKFAVRSLTYVLRKERVGRIRVTTV
                     DPGMVGETEFSDVRFRGDAERKRAVYEGVRYLTPAEVADCILWVVTRPAHMNVDEIVV
                     KPLQQATQDQIVRTSDGAAAEAAQDW"
BASE COUNT          227 a          534 c          569 g          229 t
ORIGIN      
        1 gtcctgacct cgtggacgat cttcccggct cggcgtccgg ttcggggtcg tcctcggtcg
       61 cctggagctc ggcgaggacg gcgagagcgt tccgggcgag ctccgggccg aacaggaacg
      121 tctggaggca cgtgatcagc gtgtcgcggc cgaagacggc catgaaccac ggcatcccgg
      181 ccgcgaagag ctggccctcg ggttgagcgc tccggatccg aagcgaggcg aggtccgaga
      241 ccgaccggtc gaacgccgcg gcgagcggct cccacgtggc gcgaagctgc gggatgcgca
      301 tgtgccaggc ggagagcgag gcgcgcaccc tcgcgagctc gtcgccgaac tgccgctcgg
      361 cgttctcggg ctccgtccgg ctgccgtcgg aggcgaccgt cacctccacg cgcaggcccc
      421 agctctcgcc gggctcgagc gcgatcgggt agcggacggt ggagccgtcg accttcccgc
      481 gctgcgagaa gaagagctgg gtcacgccgg cgaaggggct cgggtcgacg aggacgaggt
      541 ggccatcggc gtccgcgtcg gcgatcgtca cccgctcggg cagcgggttc gcgtggtctg
      601 ggtcgccgag cgcaaagtcg tgctccttca ccgtcaggat gtccgcgaag tcggtaccga
      661 cctcgagcgc gagctcgaag tcgagccgcc ggtgggcgtg gttttggacg acgacgtggt
      721 cctgcattcc ctcgccgacg aaccgctcgc gcgcgatcga gagctcgtcg cggtcgagac
      781 ccccggcgac ggggttgcgg aggtagtacg cggccgagaa gtactcgacc ttgtcgaacg
      841 agagcacgtg cgggcgcgcg ccgttcagcg tcaggatcca gcgggagagg aagcgggtgt
      901 cgctcgcgaa gaggccgtcc gtcgggccct cgatgtcgcc gcgctcatcc gacacgcaga
      961 aggtggagcc ctcgagaatc gtgagcgcca cgggcgctac cagtcctggg ccgcctccgc
     1021 cgcggcgccg tcgctcgtcc ggacgatctg gtcctgggtc gcctgctgga gcggcttgac
     1081 gacgatctcg tcgacgttca tgtgcgccgg ccgggtgacg acccacagga tgcagtcggc
     1141 tacctcggcg ggcgtcaggt agcggacgcc ctcgtagacc gcccgcttgc gctcggcgtc
     1201 gccgcggaag cggacgtccg agaactcggt ctcaccgacc attccggggt cgaccgtcgt
     1261 cacgcggatc cgcccgacgc gctccttccg caacacgtac gtgagcgagc ggacggcgaa
     1321 cttggaggcg acgtacatcc cgccgtaggt gtaggcctcc cggcccgccc acgagccgag
     1381 gttgacgacg tggccgcgcc ggtcctccat gtgcgggacg acgagccgcg tcatgcggac
     1441 gagaccgagg acgttcgtct cccacatcgc ggcgtcgtcg tcgtcgctcc cgtcggcgat
     1501 cgcggtccgc ccaagcgcaa ggcccgcgtt gttcaccagg atgtcgaccc cgtcgaacg
//
LOCUS       DASZOB010000017         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_101403, whole genome shotgun sequence.
ACCESSION   DASZOB010000017 DASZOB010000000
VERSION     DASZOB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101403"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VG709_00270"
     CDS             complement(<1..499)
                     /locus_tag="VG709_00270"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HWC31545.1"
                     /translation="MGTADRAKGASALLGDVTGAVADLGVLVPLAAALIVVNGLDAGA
                     VLVGAGLLVVATGIVFRVPFPVQPLKALTAVAVAERLEPDVIHAAGLEIGAFLLLLSV
                     RRVADAIARVFTKPVVRALQFGVGALLVIAATRLVVDPPDVFPGTPSSPWPILLAVAS
                     FAIVSW"
     gene            585..1040
                     /locus_tag="VG709_00275"
     CDS             585..1040
                     /locus_tag="VG709_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWC31546.1"
                     /translation="MSDGAARGTREDRSGPAVASRLSDAGFDVVRTETVSDDRDAISS
                     ALRELAEAAGLVVTTGGTGLAPSDVTPEATRDVIEREVPGLAEAMRSAGRASVPTADL
                     SRGLAGTVGSTLVVNVPGSERGAVESLGAVLEALPHALDVLAGETGHGE"
     gene            1037..1696
                     /locus_tag="VG709_00280"
     CDS             1037..1696
                     /locus_tag="VG709_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWC31547.1"
                     /translation="MRVRIKMFGALAERSRMNEAAIDFEHPATTADVVQAVRESAPEL
                     AGILERISIAVNHEISHGERPLRDGDEVALMPPVAGGAAILVGLRDHPSADDALAAVR
                     SSRAGGTAVFVGTVRDRSDGRVVDRLEYSAYEEMADAVLGDIAGAAAEKWGLEGVAVL
                     HGVGEMRVGDVTVVVACSAAHRAEALEACRWMIDEVKSRVPIWKKESGPEGSRWVGIE
                     P"
BASE COUNT          261 a          608 c          708 g          226 t
ORIGIN      
        1 cccacgacac gatcgcgaac gacgcgacgg cgagcaggat cggccacggc gaggagggcg
       61 tcccagggaa cacgtcggga gggtcgacga cgaggcgcgt cgcggcgatg accagaagcg
      121 cgccgacgcc gaactgcagc gcgcgcacga ccggcttcgt gaagacgcgc gcgatcgcgt
      181 cggcgacgcg acgaaccgag agcagaagga ggaacgcccc gatctcgagg ccggcggcgt
      241 ggatcacgtc cggctccagg cgctcggcca ccgcgacggc cgtgagcgcc ttcagcggtt
      301 ggaccgggaa cgggacgcgg aagacgatgc cggtcgcgac gacgaggagc ccggcgccca
      361 ccaggacggc gccggcgtcg agcccgttca cgacgatcag cgcggccgcg agcggaacca
      421 gtactccgag gtcggcgacg gcgccggtca cgtcgccgag cagggcgctc gcccccttcg
      481 cgcggtcggc ggtccccatc gggccctcat tctccgccgg cgatcgttcc gatggggcgc
      541 cgtatcatcc ggtcgtgggg gagcggaccg ctgcggtact tacggtgagc gacggcgcgg
      601 cgcggggtac ccgcgaggac cggtccggtc cggccgtcgc atcgcgcctc tcggacgcgg
      661 gtttcgatgt cgtccgcacc gaaacggtgt ccgacgaccg cgacgcgatc tcgtccgcgc
      721 ttcgcgagct cgccgaggcg gcggggctcg tcgtgacgac cggcggtacc gggctggcgc
      781 cgagcgacgt gacgcccgag gcgacgcgcg acgtgatcga gcgcgaggtc cccgggctcg
      841 cggaggcgat gcggtcggcg gggcgtgcat cggtgccgac cgcggacctc tcacggggcc
      901 tcgccggcac cgtcggctcg acgctcgtcg tcaacgtccc cgggagcgag cgcggcgccg
      961 tcgagagcct cggcgcggtg ctcgaagcgc tgccccacgc gctcgatgtc ctcgccggcg
     1021 agacggggca cggcgagtga gggtccggat caagatgttc ggggcgctcg cggagcgttc
     1081 gcggatgaac gaggcggcga ttgacttcga gcatcccgcg acgacggcag acgtcgtaca
     1141 ggcggtacgc gagtcggctc cggagctcgc ggggatcctc gagcgcatct cgatcgccgt
     1201 gaaccacgag atctctcacg gcgagcgacc gcttcgcgac ggcgacgagg tcgcgctgat
     1261 gccgccggtc gccggcggcg cggcgattct cgtcggtctc cgcgaccatc cctcggcgga
     1321 cgacgccctc gcggccgtgc gttcctcgcg cgccggcgga accgccgtct tcgtcgggac
     1381 ggtccgcgac cgctccgacg gacgtgtcgt cgaccgtctc gagtactcgg cgtacgagga
     1441 gatggccgac gccgtgctcg gggacatcgc cggcgcggcg gcggagaagt ggggactcga
     1501 gggcgtcgcg gttctccacg gtgtcggcga gatgcgcgtc ggcgacgtca cggtggtcgt
     1561 ggcgtgctcg gcggcgcacc gcgccgaggc gctcgaggcg tgtcggtgga tgatcgacga
     1621 ggtgaagtcg cgcgtgccga tctggaagaa ggagagcgga cccgaggggt cgcgatgggt
     1681 tgggatcgag ccgtgacgga gccggcccgg atcgtcgacg tgggcgagaa ggacgtcacc
     1741 gcgcgagagg cgacggccgc gtgcacgttc tcttcgaagg ccgtcgcacg actcacggag
     1801 cga
//
LOCUS       DASZOB010000018         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_121556, whole genome shotgun sequence.
ACCESSION   DASZOB010000018 DASZOB010000000
VERSION     DASZOB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121556"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VG709_00285"
     CDS             <1..253
                     /locus_tag="VG709_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31548.1"
                     /translation="FTVSATNSQIQTTVAERIRARVVGLFLLAFGGLFPLASLIAGTI
                     ADAIGAPLTTAGGAVICLGWGLSLLWTWRERSPAVERSA"
     gene            complement(263..754)
                     /locus_tag="VG709_00290"
     CDS             complement(263..754)
                     /locus_tag="VG709_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWC31549.1"
                     /translation="MGREPEALTEPSPAAQGTVAPHRILVAADGSDGAGRATRLAVRF
                     LRGDRVEARVLTVLSFEMDPYTLLGEVTPETQERLEVVDRAVERATEAPRRILEEAGH
                     RVAVVHRFGNPADEILADIDEWGPDLVVIGRRGLAAPSRWLLGSVSERVLRHGHVPVL
                     VGP"
     gene            complement(754..1245)
                     /locus_tag="VG709_00295"
     CDS             complement(754..1245)
                     /locus_tag="VG709_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWC31550.1"
                     /translation="MESEVSVFVEIPAGSRNKYETDPETGNLFLDRTLFTATRYPADY
                     GFIEGTLGEDGDALDALVFVGEPTFPGCRITARPVGLFRMWDEKGPDEKILCVPLRDP
                     AWSHVERLGDLRPTLRAEVEHFFQVYKDLEGKPTGTEGFGERDEALQVIEAARRRAAT
                     GGA"
     gene            1354..1776
                     /locus_tag="VG709_00300"
     CDS             1354..1776
                     /locus_tag="VG709_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HWC31551.1"
                     /translation="MLTVSRLARSAGVRPDTIRYYDREGLLPVATRTPSGYRVYEEDA
                     VRRLRFIRGSQRLGLRLREIKGLLAVIDRGTCPCGHARAVLDERLAEIDRQLAQLSAM
                     RAELVRVSEKVGPDTCTPGVWPCEEELIEVGGGDASGP"
     gene            complement(1830..>2023)
                     /locus_tag="VG709_00305"
     CDS             complement(1830..>2023)
                     /locus_tag="VG709_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HWC31552.1"
                     /translation="WVQNIQQFRWDEEEVNKELRKRMATAWKAVHSRSTVDGIPLRLA
                     AFAIAVERVDRAARLRGYV"
BASE COUNT          280 a          734 c          698 g          311 t
ORIGIN      
        1 gttcacggtc tcggcgacga acagccagat ccagacgacc gtcgccgagc ggatccgcgc
       61 gcgcgtcgtc gggctgtttc tgctcgcatt cggcgggctg ttcccgctcg ccagcctcat
      121 cgccggcacg atcgccgacg cgatcggcgc cccgcttacg acggccggtg gcgccgtgat
      181 ctgcctcgga tgggggctgt cgctgctgtg gacgtggcgc gagcgctctc cggccgtgga
      241 acgcagcgcc tgagccgacg ggtcagggcc ccacgaggac cggcacgtgg ccgtggcgga
      301 gcacgcgttc ggagacgctc ccgagaagcc accgcgacgg cgccgcgagc ccgcggcgtc
      361 cgatcacgac gaggtccgga ccccactcgt cgatgtccgc caggatctcg tcggcggggt
      421 tgccgaaccg gtggacgacg gcgacccgat gccccgcttc ctcgaggatg cgccgcggcg
      481 cctcggtcgc tcgctccacc gcacggtcga cgacctcgag tcgctcctgc gtctccggcg
      541 tgacctcgcc gagcagcgtg tagggatcca tctcgaacga gagcacggtc aggaccctcg
      601 cctccacccg gtcaccgcga aggaagcgca ccgcaagccg ggtcgcgcgg cccgcgccgt
      661 cggacccatc cgccgcgacg agtatgcggt gaggggcgac ggtaccctgg gccgcgggcg
      721 acggctccgt cagcgcctcg ggctcgcgcc ccatcacgcc cctcccgtcg cggctctccg
      781 ccgagccgcc tcgatcacct gcagcgcctc gtcccgttcc ccgaacccct cggtgcccgt
      841 cggcttcccc tcgagatcct tgtagacctg gaagaagtgc tcgacctcgg cgcggagcgt
      901 cggtcggagg tccccgaggc gttcgacgtg cgaccatgcg gggtcgcgca gcggcacaca
      961 gagaatcttt tcgtcggggc ccttctcgtc ccacatccga aacagcccga cgggacgggc
     1021 cgtgatgcgg catccaggaa acgtcggctc gccgacgaac acgagcgcgt ccagcgcgtc
     1081 gccgtcctcg ccgagcgtcc cctcgatgaa cccgtagtcc gccggataac gcgtcgccgt
     1141 gaacagcgtg cggtcgagga acaggttccc ggtctcggga tccgtctcgt acttgttgcg
     1201 cgatccggcc gggatctcta cgaaaaccga gacctcggat tccatgggag aactctacgc
     1261 acctcgcgga aaccggtcgc cgcctcggtc gcgttcgagc gccgagacgt cgcccttgac
     1321 cttggagcgc actccagggt ttaccctggg ggcgtgttga cggtctcgag gctcgcgcgg
     1381 tcggccgggg tgcgccccga cacgatccgc tactacgatc gggagggtct gcttcctgtc
     1441 gcgacccgga cgccgagcgg ctaccgcgtg tacgaggagg acgcggtccg gcggctccgc
     1501 ttcatccggg ggtcgcagcg tctcgggctg cggctgcgcg agatcaaggg gctgctcgcc
     1561 gtgatcgatc gcggcacctg cccgtgcggt cacgcgcggg cggtcctcga cgagaggctg
     1621 gctgagatcg accgccagct cgcgcagctt tcggccatgc gtgcggagct cgtgcgcgtc
     1681 tccgagaagg tcgggcccga cacctgcacg ccgggggtct ggccgtgcga ggaggagttg
     1741 atcgaggtgg gaggaggtga cgcgagtgga ccgtgacccc gcaaacccgg agccgtgctg
     1801 cccggactgc ccgccgtact gctgctagct cagacgtagc cgcgcagccg cgcggctcgg
     1861 tccacgcgct cgacggcgat cgcgaacgcg gccaggcgga gcgggatccc gtcgaccgtc
     1921 gagcgcgagt ggacggcctt ccaggccgtc gccatcctct tccgcagctc cttgttgacc
     1981 tcttcctcgt cccagcggaa ctgctggatg ttctggaccc act
//
LOCUS       DASZOB010000019         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_192337, whole genome shotgun sequence.
ACCESSION   DASZOB010000019 DASZOB010000000
VERSION     DASZOB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192337"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VG709_00310"
     CDS             complement(<1..283)
                     /locus_tag="VG709_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC31553.1"
                     /translation="MKTLVAGATGDIGVPVVRRLLDRGHDVVGITRSEQNRARLERMG
                     ARGVVASVLDRDALPGLVEDAQPDAVVDLLTALPRTGPRRPSHLPAPGRR"
     gene            complement(280..678)
                     /locus_tag="VG709_00315"
     CDS             complement(280..678)
                     /locus_tag="VG709_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31554.1"
                     /translation="MGHRGVPGRRGRSLAWLAAIAGVIFLGIGAWAFADPRSFFERIA
                     TFPPYNEHFLHDIGSFVIGLGAVLLISAFVRDSLVTAFGGVGIGQAGHAAAHAIDRDL
                     GASPGDPWIGGVAALALLGAAAWRLATLER"
     gene            703..>2005
                     /locus_tag="VG709_00320"
     CDS             703..>2005
                     /locus_tag="VG709_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC31555.1"
                     /translation="MNLARLGEQNVDRFGEYESLFFEGRWYRNTEWRADGSRLANVVT
                     SLGVRPGDRVALLLPNSLEVFQAYAGIAAAGAVAVPILFLLAAPEVRHLVADSRPRLV
                     VTAPLFLDKVREALDGLDDPPRVVVVGEAEGHDVLLWSMLMADAEPEFTIVDRADDDL
                     AMIMYTGGTTGRPKGVMLTHGNLHWNAVTVADAVRLDRDTVSLLCLPVAHLFGAFAAI
                     LGQVLGLRGVMLEWFTPDGALQAIEAHGVSYVAMVPTMMTLLLQHPDGERYDTSSLRT
                     VFASAAPVPIELAEAFEKRFGCEVLEGYGQTEAAPAIALMRPGLPKKAGSTGPPVPGV
                     DVRIEDDDHRAVPPGELGEICARSPGSMAGYHNLPDVTEATLRHGWLHTGDVGYLDDD
                     GYLFVTDRKRDLVIRGGLNVYPRDIEEVLLEHPGVAAAAVVG"
BASE COUNT          289 a          731 c          706 g          279 t
ORIGIN      
        1 aacgtcgtcc gggcgcgggg aggtgtgacg gtcggcgcgg tcccgtgcgc ggcagcgcgg
       61 tgagaaggtc gaccacggcg tcgggctgcg cgtcctcgac gagacccgga agggcatcgc
      121 gatcgagcac gctcgcgacg accccgcgcg cacccatccg ctcgaggcgc gctctgttct
      181 gctccgaacg cgtgatgccc acgacgtcgt ggcctcggtc gaggagccga cggaccacgg
      241 gcaccccgat gtcgccggtg gcgccggcga cgagcgtctt catcgctcga gggtcgccag
      301 acgccacgcg gcggcgccga gcagcgcgag cgcggcgacg ccgccgatcc acggatcgcc
      361 cgggctcgcg ccgaggtccc ggtcgatcgc gtgcgcggcg gcgtggcccg cctggccgat
      421 ccccacgccg ccgaacgccg tgaccagtga gtcgcggacg aacgccgaga tcaggagcac
      481 ggcgccgagg ccgatcacga acgagccgat gtcgtggagg aagtgctcgt tgtacggcgg
      541 gaacgtcgcg atccgttcga agaacgacct cgggtcggcg aacgcccacg ctccgatccc
      601 gaggaagatc acgccggcga tcgctgcgag ccatgcgagg ctgcgacctc gtcgccccgg
      661 taccccgcgg tgtcccacgg cggcatccta gacttcgcgc cgatgaacct cgcgcggcta
      721 ggggagcaga acgtcgaccg cttcggcgaa tacgagtcgc tcttcttcga gggacgctgg
      781 taccgcaaca ccgagtggcg cgccgacggc agcaggctcg cgaacgtcgt cacgtcgctc
      841 ggcgtccgtc ccggcgaccg cgtcgcgctg ctccttccga actcgctcga ggtgttccag
      901 gcgtacgcgg ggatcgcggc tgccggagcc gtcgcggtcc cgatcctgtt cctgctcgct
      961 gcgcccgagg tgcgtcacct ggtcgcggac tcgcgtccga ggctcgtcgt gacggcgccg
     1021 ctcttcctcg acaaggtccg cgaggcgctc gacggcctgg acgacccacc gcgagtcgtc
     1081 gtcgtggggg aagccgaggg ccacgacgtc ctcttgtggt cgatgctcat ggccgacgcc
     1141 gaaccggagt tcacgatcgt cgaccgtgcc gacgacgatc tcgcgatgat catgtacacg
     1201 ggcgggacga cggggcgccc caagggcgtg atgctcaccc acgggaacct ccactggaac
     1261 gccgtcaccg tcgccgatgc cgtccggctc gaccgcgaca ccgtctcgct cctctgcctc
     1321 ccggtcgcac acctcttcgg cgcgttcgcg gcgatcctcg gccaggtgct ggggctccga
     1381 ggcgtgatgc tcgagtggtt cacgccggac ggcgccctgc aggcgatcga ggcgcatggc
     1441 gtcagctacg tcgcgatggt cccgacgatg atgacgctgc tgctccagca ccccgacgga
     1501 gagcggtacg acacctcgtc cctccggacc gtcttcgcgt ccgcggcgcc cgtgccgatc
     1561 gaactcgccg aggcgttcga gaagcgcttc gggtgcgagg tcctcgaggg ctacggccag
     1621 accgaggccg cgccagcgat cgcgctcatg cgccccggcc tcccgaagaa ggccggctcg
     1681 acgggcccgc ccgtgccggg cgtcgatgtc cggatcgagg acgacgacca ccgggcggtt
     1741 ccgccggggg agctcggcga gatctgcgcg cggagccccg ggagcatggc ggggtaccac
     1801 aacctccccg atgtcaccga ggccacgctc cggcacgggt ggctccacac cggcgacgtc
     1861 ggctacctcg acgacgacgg ctacctgttc gtgaccgacc gcaagcgcga tctcgtgatc
     1921 cgcggcggcc tcaacgtcta cccgcgcgac atcgaggagg tgctgctcga gcaccccggc
     1981 gtcgccgcgg cggccgtcgt cggac
//
LOCUS       DASZOB010000020         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_20640, whole genome shotgun sequence.
ACCESSION   DASZOB010000020 DASZOB010000000
VERSION     DASZOB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20640"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1554
                     /locus_tag="VG709_00325"
     CDS             31..>1554
                     /locus_tag="VG709_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWC31556.1"
                     /translation="MVWTTDEDLRVTAAFGEGISGLEVHPRELIGLMIDEILGHDPAA
                     PAVAAHERALRGAPDTYLQEFMGRTFQCRVEPLRQAGRGDGVIGVALDITERAEAERR
                     VMEAELRYRSLVEQGPAVVYSSPARGRDAPEFASPQIEDLLGRSPDEWARDPALWRRS
                     IHPEDRERVAAEWEKAVAGEGAFESEYRMIHADGRVVWVRDQSRLVRDSERRALGWQG
                     VFTDITRARRREQQLRRTNQALRALIEASPLAIAAVDLDGNVTLWNPAAERLFGWTEG
                     DVVGKPIPTIPSRVREEWTVVLDSGSQGAVVSTPETRCLRKDGSEISVEISRAPIRDA
                     SGEIVGIFGIFTDITERKRAEQALRASEERYRGLFENALDVVFTTDLTGRFTAVNRAA
                     KRVSGYSRKELLRMNIIDLVASEHRDVVQRVLDLTPPVAQAADAPRARTRYELDIVTK
                     EGRRRSLEAVIHLVWEGDEPVAVEGIARDVTDRKHADEALRASEERYRELFENARDII
                     "
BASE COUNT          258 a          521 c          558 g          217 t
ORIGIN      
        1 ctccggctct tcgccgactt cttcccgacg atcgtctgga cgaccgacga ggacctgcgc
       61 gtaacggcgg cgttcggtga gggcatcagt ggtctcgagg tgcacccgcg tgaattgatc
      121 ggcctcatga tcgacgaaat cctcggccac gaccccgcgg cacccgcagt cgccgcgcac
      181 gagcgcgcgc tgcgcggggc ccccgacacg tacctccagg agttcatggg tcggacgttc
      241 cagtgccgcg tcgaacccct gcgacaggcg ggacggggcg acggcgtgat cggcgtcgcc
      301 ctcgacatca cggagcgcgc cgaggccgaa cggcgggtga tggaggcgga gcttcgctac
      361 cgctcgctgg tcgagcaggg tccggcggtc gtctacagct cacccgctcg cgggcgggac
      421 gctccggagt tcgcgagccc gcagatcgag gatctcctcg ggcggagccc tgacgagtgg
      481 gcccgcgatc cggcgctctg gaggcggtcc atccatcccg aggatcggga gcgggtcgcc
      541 gccgagtggg agaaggccgt cgccggcgag ggcgccttcg agagcgagta ccggatgatc
      601 catgcggacg gccgcgtggt gtgggtccgc gaccagtcgc gtctcgtccg cgactccgag
      661 cggcgagcgc tgggatggca gggcgtcttc acggacatca cgcgcgcgcg gcgccgggag
      721 cagcagctcc ggcggaccaa ccaggcgctt cgggcgctga tcgaggcctc accgctggcg
      781 atcgcggccg tcgacctcga cgggaacgtc acgctgtgga acccggcggc cgagcgactg
      841 ttcgggtgga ccgaaggcga cgtcgtcggg aagcccatcc ccacgatccc ctcgcgggta
      901 cgcgaggaat ggacggtcgt gctcgactcc ggctcgcaag gagccgtggt ctcgaccccg
      961 gaaacccggt gcctccgcaa ggacgggtcc gagatctccg tcgagatctc gcgcgccccg
     1021 atacgcgatg cgagcggcga gatcgtgggg atcttcggga tcttcaccga tatcaccgaa
     1081 cgaaagcgtg cagagcaggc gctccgtgcg agcgaagaac gctaccgagg cttgttcgag
     1141 aacgcactcg acgtcgtctt cacgacggac ctcaccggaa ggttcacggc cgtgaaccgc
     1201 gccgccaagc gcgtgagcgg gtactcacgc aaggagctgc tccggatgaa catcatcgat
     1261 ctcgtcgctt cggagcatcg cgacgtcgtc caacgggtgc tcgacctcac cccgcccgtc
     1321 gcgcaggcgg ccgatgcacc gcgcgcccgg acccgctacg agctcgacat cgtgacgaag
     1381 gaggggaggc gacgctccct ggaggccgtg atccatctcg tgtgggaagg ggatgagccc
     1441 gtcgcggtcg agggcatcgc cagggacgtc accgaccgga agcacgccga tgaggcgctc
     1501 agggcgagcg aggagcggta ccgagagctc ttcgagaacg cgcgggacat catc
//
LOCUS       DASZOB010000021         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10367, whole genome shotgun sequence.
ACCESSION   DASZOB010000021 DASZOB010000000
VERSION     DASZOB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10367"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VG709_00330"
     CDS             <1..501
                     /locus_tag="VG709_00330"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HWC31557.1"
                     /translation="ADGRHEEKPARDPHHDGRDPLVLQRPGSRVRPPPPGPRNAARPM
                     RTGARFFLISAAFAATIASAYWFVTYEPAGTALLGSLTLAALVAAAYVTRSARSRREP
                     EDRPDATPSAAEGRTFGPMFATSAWPVLVGAGAMLLAGGLVYAPWLLVVGAVAFALGL
                     LGLARE"
     gene            complement(429..1247)
                     /locus_tag="VG709_00335"
     CDS             complement(429..1247)
                     /locus_tag="VG709_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWC31558.1"
                     /translation="MNVGVVATVALAGAAYAAGVRRFDASHPRERFARVRAWSFAAGL
                     AVVLVALVGPLDDAAARRFSAHMGQHLLLTLVAPPMLLWGRPLMLARRATPPRVRRAV
                     ASTLRTGAARTLAHPVVAWSALALAMWASHFTSLYEAALRSDAVHAAEHALYLGAGVL
                     FWLPVVAVEPSPWRLDHAARVLYIFLALPANALLAVALYESRTVLYASYAGTGALEDQ
                     RVAAVVMWVAGGLLLVAAVLLVTVRWARAERYSRASPSRPSAKATAPTTSSHGA"
     gene            complement(1244..>1727)
                     /locus_tag="VG709_00340"
     CDS             complement(1244..>1727)
                     /locus_tag="VG709_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWC31559.1"
                     /translation="YLGWLEGALRLFPPVAFELFDRLIAEPFVPGVVLPGTFFTAMFL
                     WPFIERRITGDTETHHVLDYPRDAPMRTAVGVGALAFTAILTLAGSNDVLGAFFGVPV
                     ETVTRVLRVLVLLLPLVAAFFAYRLCRSLRDRGVHPLSDPERVRLRQDARGGFREDHA
                     "
BASE COUNT          269 a          653 c          626 g          179 t
ORIGIN      
        1 gcggacggcc gccacgagga gaagcccgcc cgcgacccac atcacgacgg ccgcgacccg
       61 ctggtcctcc agcgccccgg ttcccgcgta cgacctccgc ctcccggacc ccgaaacgcc
      121 gcccgaccga tgagaacggg cgcgcggttc ttcctcatct cggccgcgtt cgccgcgacg
      181 atcgcgtccg cgtactggtt cgtcacgtac gagcccgccg gcacggcgct cctcgggagc
      241 ctcacgctcg cggcgctcgt cgccgcggcg tacgtgacga ggagcgctcg gtctcggcgc
      301 gaacccgaag accgcccaga cgccacgccg tcggcggcag agggacgcac gttcggcccc
      361 atgttcgcga ccagtgcctg gccggtcctc gtgggcgcgg gcgcgatgct cctcgccggc
      421 gggctcgtct acgcgccgtg gctgctcgtc gtgggcgccg tggccttcgc gctcggcctg
      481 ctcgggctgg cacgcgaata gcgctccgcc cgagcccacc tcacggtgac gaggaggacg
      541 gccgccacga ggagaagccc gcccgcgacc cacatcacga cggccgcgac ccgctggtcc
      601 tccagcgccc cggttcccgc gtacgacgcg tagagcaccg tccgcgactc gtagagcgcg
      661 acggcgagga gcgcgttcgc ggggagcgcg aggaagatgt acaggacgcg cgcggcgtgg
      721 tcgaggcgcc acggcgacgg ctcgaccgcc acgaccggga gccagaacag cacgccggcg
      781 ccgaggtaca gcgcgtgctc ggccgcatgc acggcgtcgc tgcggagcgc cgcctcgtac
      841 agcgacgtga agtgcgacgc ccacatcgcg agcgccagcg ccgaccacgc caccaccggg
      901 tgggccagcg tcctcgccgc gcccgtgcga agcgtcgacg cgacggcccg acgcacgcgc
      961 gggggggtgg cccggcgcgc gagcatgagc ggccgtcccc agagcagcat cggcggagcg
     1021 accagcgtga gcagcaggtg ctgtcccatg tgcgccgaga accgcctggc cgccgcgtcg
     1081 tcgagcggac cgacgagcgc cacgaggacg accgcgagcc ccgcggcgaa cgaccacgca
     1141 cggacgcgcg cgaaccgctc tcgcgggtgc gaggcgtcga accggcgcac gcctgcggca
     1201 tacgccgcgc cggcgagcgc gactgtggcg acgacgccga cgttcacgcg tggtcctcgc
     1261 ggaagccgcc ccttgcatcc tgacggaggc ggacgcgctc cgggtcggac agcggatgga
     1321 caccgcggtc gcgcagcgag cggcagaggc ggtacgcgaa gaatgccgcc acgagcggca
     1381 ggaggagcac gagcacgcgg aggacgcgcg tgacggtctc gaccggcacg ccgaagaacg
     1441 ccccgagcac gtcgttgctc cccgcgagcg tcaggatcgc cgtgaacgcg agcgcgccga
     1501 cgccgaccgc cgtccgcatc ggggcgtcgc gcgggtagtc gagcacgtga tgcgtctcgg
     1561 tgtcgccggt gatccgccgc tcgatgaacg gccacaggaa catggccgtg aagaatgtcc
     1621 ccggcagcac gacgcccggg acgaacggct ccgcgatcag ccggtcgaac agctcgaacg
     1681 ccacgggtgg gaatagccgc agcgcgccct cgagccatcc gaggtac
//
LOCUS       DASZOB010000022         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131517, whole genome shotgun sequence.
ACCESSION   DASZOB010000022 DASZOB010000000
VERSION     DASZOB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131517"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="nuoD"
                     /locus_tag="VG709_00345"
     CDS             complement(<1..1224)
                     /gene="nuoD"
                     /locus_tag="VG709_00345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWC31560.1"
                     /translation="MGTGVTTQRLRDDLLVVNMGPQHPSTHGVLRLLLTLDGETIVDC
                     QPIVGYLHTGIEKNAEYRTWQQGSTFVTRMDYLSPFFNELGYCLSVERLLGIEAPARA
                     EAIRVLLCEMTRISSHLVWLATTSLELGAISMMLYGFRERELILDIFEMITGLRMNHA
                     YFRVGGLLMDLPEGTEEKIRAFLRHMPGRIDEYETLLSENPIWVERNKGVGYLSAETC
                     LAYGVTGPLLRAAGVAHDIRKSEPYCGYEQYEFAVPTSSEADCYARYEIRIAEMRESL
                     RICEQVLDSLPGGRVMIDDPKLAWPAHLSVGPDGIGNSEEYVRHIMEESMEALINHFK
                     IVTQGIQVPAGEVYTAVESPRGELGYYVVSDGGVRPYRVRVRDPSFVNLPVLPEMVRG
                     RLIADTIACVASVDPV"
     gene            complement(1325..1822)
                     /locus_tag="VG709_00350"
     CDS             complement(1325..1822)
                     /locus_tag="VG709_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWC31561.1"
                     /translation="MRADDLADKLRQRWRDVVVARGEATVTVDRGELLAALEYVRDEE
                     DLSLRFLADVSCSDWPARDPRIWMAYHLLSLEHGHRLRVKVGLAGDDLRIPSVVPIHP
                     TANWQEREVFDMFGVTFEGHPDLRRILMPEDWEGYPLRKDHALGGVATQYKGATVPPP
                     DQRGL"
     gene            complement(1855..2328)
                     /locus_tag="VG709_00355"
                     /pseudo
     CDS             complement(1855..2328)
                     /locus_tag="VG709_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(2325..2687)
                     /locus_tag="VG709_00360"
     CDS             complement(2325..2687)
                     /locus_tag="VG709_00360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWC31562.1"
                     /translation="MTLGDYLPIVLLAALGLVFAALSLLASRLVQPRRPTPAKLAPYE
                     CGIVPEQSPKGERFPVKFYVIAMLFIVFDIEAIFLFPWAVTFRQLGLFGLVEMVIFIA
                     LVFVAYVYVWQRGGLEWE"
     gene            complement(2684..3982)
                     /locus_tag="VG709_00365"
     CDS             complement(2684..3982)
                     /locus_tag="VG709_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWC31563.1"
                     /translation="MKGFTSPAIQPARGEPVRDATGRVTEVDVLVVGAGPAGAASAYH
                     LARHGVDVLVVDKARFPREKVCGDGLTPRGVAAAARMGVDMTEPGFVRGDGLRVFGPT
                     VRLDLPWPKLRRFPDYGVVRTRHDFDNLLVERATKAGAMLWEGAEALAPLTDDGWVVG
                     ARVRREDAEPETVRAKFVLACDGASSRFAMKAGVRRDPSRPLGVAARRYFRSPREQPP
                     YFESYFNLWENGVILPGYGWIFPVGDGVLNVGAGLLNTTSMFKDVSPRRMLDVFLGNL
                     PPEWGITEENADGPLLSGPLPMGFNRSPLALPGLLLVGDAGGLINPFTGEGIAYAIES
                     GELAAELVHEALATGRPGLAHVYPIALRERYGKYFTAGRAFARAIGNPRIMRYSVLHG
                     FPRERLMRFAIRLLSNLTDGPSGDLDDRVMDLIVRMAPAR"
     gene            complement(4031..>4284)
                     /locus_tag="VG709_00370"
     CDS             complement(4031..>4284)
                     /locus_tag="VG709_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31564.1"
                     /translation="TGRRWQELAGATVWGRAEPLVPEHPALRGVMSGWFEKYRLMLAG
                     GGFRDYAERVESPGIIRVRPERIAAWDHRAATLPRAARG"
BASE COUNT          685 a         1542 c         1434 g          623 t
ORIGIN      
        1 caccggatcg acgctcgcga cgcatgcgat cgtgtcggcg atcagccggc cgcgcaccat
       61 ctcggggagc accgggaggt tcacgaacga ggggtcccgc acgcggacgc gatacgggcg
      121 gacgccgccg tcggaaacga cgtagtagcc cagctcgccg cgcggggact cgaccgccgt
      181 gtacacctcg ccggcgggga cctggatccc ctgggtcacg atcttgaagt ggttgatgag
      241 cgcctccatc gactcctcca tgatgtggcg cacgtactcc tcggagttgc cgatgccgtc
      301 cggcccgacc gagaggtgcg cgggccatgc gagcttcggg tcgtcgatca tcacgcgtcc
      361 accgggaagc gagtcgagca cctgctcgca gatccggagc gactcacgca tctccgcgat
      421 tcggatctcg tagcgcgcgt agcagtccgc ctcggagctg gttggaacgg cgaactcgta
      481 ctgctcgtac ccgcagtacg gctccgactt ccggatgtcg tgcgcgacgc cggcggcgcg
      541 caggagcggg cccgtgacgc cgtacgcgag gcacgtctca gccgagaggt acccaacgcc
      601 cttgttgcgc tcgacccaga tcgggttctc ggacagcagg gtctcgtact cgtcgatgcg
      661 gcccggcatg tgtcgcagga aggcgcggat cttctcctcc gtcccttccg gaaggtccat
      721 caacaggccg ccgacgcgga agtacgcgtg gttcatccgc aggcccgtga tcatctcgaa
      781 gatgtcgagg atgagctccc gctcccgaaa gccgtacagc atcatcgaga tcgcgccgag
      841 ctcgagcgag gtcgtcgcga gccacacgag gtggctcgag atgcgcgtca tctcgcacag
      901 cagcacgcgg atcgcctccg cgcgcgccgg cgcctcgatg ccgaggagcc gctccaccga
      961 caggcagtaa cccagctcgt tgaagaacgg cgagagatag tccatccgcg tcacgaacgt
     1021 ggaaccctgc tgccaggtcc ggtattcggc gttcttctcg atgccggtgt ggaggtagcc
     1081 gacgatcggc tggcagtcga cgatcgtctc gccgtcgagc gtgagcagca gccggagcac
     1141 gccgtgcgtc gacgggtgct gcggccccat gttgacgacg agcagatcgt cgcggagccg
     1201 ctgcgtcgtg accccggtcc ccatagaggg accggctatg tcctcgggca gccgctcggc
     1261 ccccgtacgg tcgctgcgct cgacgcccgc catcagacgc tcgcctccgg ctcgacgccc
     1321 gccatcacaa ccctctctgg tccggcggcg ggaccgtggc gcccttgtac tgggtcgcga
     1381 ccccaccgag cgcgtggtcc ttgcgcagcg gatagccctc ccagtcctcc ggcatcagga
     1441 tccggcggag gtcggggtgg ccctcgaacg tcacgccgaa catgtcgaag acctctcgct
     1501 cctgccagtt ggcggtcggg tggatcggga ccacagacgg gatgcggagg tcgtcgccgg
     1561 cgaggccgac cttcaccctg aggcggtggc cgtgctcgag cgacaacagg tggtacgcca
     1621 tccagatcct cggatcgcgt gcgggccagt cgctgcacga gacgtccgcg aggaaccgca
     1681 gcgagagatc ttcttcgtca cgcacgtact cgagcgccgc gaggagctcg ccacggtcga
     1741 ccgtcacggt cgcctcgccg cgcgcgacga cgacgtcacg ccaccgctgc cgcagcttgt
     1801 ccgcgaggtc gtctgcccgc acgttgcccc ctagtggagg gttccccgct tcgcgatctt
     1861 ctcgtggagc tgcaggatcc cgtacatcag catctccgga cgcggcgggc accccggaac
     1921 gtagatgtcc acggggacca ccgtgtcgac gccctgaacg agcgcgtagt tgttgaacat
     1981 cccgcccgac gacgcgcaaa cgcccatcga gatgacccac ttcggctccg gcatctggtc
     2041 gtagatctgg cgcaggaccg gcgccatctt ctgcgacacc cgcccggcga cgatcatgag
     2101 gtccgcctgg cgcggcgacg cgcggaagat ctccatcccc cagcgggcga ggtcgtactt
     2161 gcccgcgccg gccgccatca tctcgatcgc gcagcacgcg agcccgaacg tcaccggcca
     2221 caccgactgc ttccgcgccc agttcacggc cttcgcgagc gtcgtgagca gcagcccctg
     2281 cttcagctcc tcctcggccg tgaggcggtc gatctcgtcg cgcgtcattc ccattcgagg
     2341 cctccgcgct gccatacgta cacgtacgcc acgaaaacga gcgcgatgaa gatcaccatc
     2401 tcgacgagcc cgaagagccc gagctggcgg aacgtcaccg cccacgggaa caggaagatc
     2461 gcctcgatgt cgaagacgat gaacagcatc gcgatcacgt agaacttcac cgggaaccgt
     2521 tcgcccttcg ggctctgctc ggggacgatc ccgcattcgt acggggcgag cttggcgggg
     2581 gtcggacgcc gcggctggac gagcctcgac gccagaaggg acagggccgc gaagacgaga
     2641 ccgagcgcgg ccagcagcac gatcggcagg tagtcgccga gcgtcatcgc gcgggcgcca
     2701 tcctgacgat gaggtccatc acgcggtcgt cgaggtcgcc actcggaccg tcggtgaggt
     2761 tcgagagcag ccgtatcgcg aagcgcatga ggcgctcgcg cgggaagccg tgcagcacgg
     2821 agtaccgcat gatgcgcggg ttcccgatcg cgcgcgcgaa cgcgcggcct gccgtgaagt
     2881 acttcccgta gcgctcgcgg agcgcgatcg ggtagacgtg cgcgagtccc ggacggcccg
     2941 tcgcgagcgc ctcgtgcacc agctcggcgg cgagctcgcc cgactcgatc gcgtacgcga
     3001 tcccctcccc ggtgaacggg ttgatgagcc cgccggcgtc gccgacgaga aggagccccg
     3061 ggagggcgag cggcgaacgg ttgaacccca tcggcagcgg acccgacagc agcgggccgt
     3121 ccgcgttctc ctcggtgatc ccccattccg gcgggaggtt gccgaggaag acgtcgagca
     3181 tccgtcgcgg cgagacgtcc ttgaacatcg aggtcgtgtt gagcaggccc gcgcccacgt
     3241 tcagcacgcc gtcgccgacc gggaagatcc acccgtaccc ggggaggatc acgccgttct
     3301 cccaaaggtt gaagtacgac tcgaagtacg gcggctgctc gcgcgggctc cggaagtacc
     3361 ggcgagcggc gacgccgagc ggtcggctgg ggtcgcggcg cacacccgcc ttcatcgcga
     3421 accgtgacga cgcgccgtcg cacgccagca cgaacttcgc acggaccgtc tcgggctccg
     3481 cgtcctcgcg ccgcacacgc gctccgacga cccagccgtc gtcggtgagc ggcgccagcg
     3541 cctcggcgcc ctcccacagc atcgcgccgg ccttcgtcgc gcgctcgacg agcaggttgt
     3601 cgaagtcgtg gcgcgtccga acgacgccgt agtcggggaa gcgccgcagc ttcggccacg
     3661 gaaggtcgag ccgaaccgtc gggccgaaga cgcgcagccc gtcgccgcgc acgaaccccg
     3721 gctccgtcat gtcgacgccc atccgcgcgg ccgcggcgac gccgcggggc gtgagcccgt
     3781 cgccgcagac cttctccctc gggaaccgcg ccttgtcgac gacgagcacg tcgacgccgt
     3841 ggcgcgcgag gtggtaggcc gatgccgctc cggcgggacc ggctccgacg acgagcacgt
     3901 ctacctcggt cacgcgcccg gtcgcgtcgc ggacgggctc accccgcgcc ggctggatcg
     3961 cggggcttgt gaaccctttc acaaagtcga tgctatcggg tagtccgcgc tcgtcgcaaa
     4021 cgggtggggg ctatccgcgg gccgcacgcg gcagcgttgc cgctctgtgg tcccaggccg
     4081 cgatgcgctc gggccggacc cggatgatcc ccggactctc cacccgctcg gcgtagtcgc
     4141 ggaacccgcc gccggccagc atcagccggt acttctcgaa ccaccccgac atcacgccgc
     4201 gcagcgcggg atgctcggga accagcggct ccgcgcgccc ccacacggtc gcgcccgcga
     4261 gctcctgcca cctgcgtccc gtag
//
LOCUS       DASZOB010000023         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_71295, whole genome shotgun sequence.
ACCESSION   DASZOB010000023 DASZOB010000000
VERSION     DASZOB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71295"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VG709_00375"
     CDS             complement(<1..682)
                     /locus_tag="VG709_00375"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWC31565.1"
                     /translation="MKVRKAVVPAAGLGTRFLPATKAQPKEMLPVVDKPAVQYVIEEA
                     VRADITDILIVTGRGKRTLEDHFDRSFELEHYLERSGKHDELKAVREITEMATVQYIR
                     QRDPAGLGDAVATAEPHVGAEPFAVLLGDDIVLSADPLLGRMVDIYERYGRSVIAVQE
                     VPREDIRLYGAVHPEFVEDNLARVVDIVEKPAPEDAPSNLAAIGRYVLTPEIFDAIRA
                     TPPDAGGEV"
     gene            complement(747..>2322)
                     /locus_tag="VG709_00380"
     CDS             complement(747..>2322)
                     /locus_tag="VG709_00380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWC31566.1"
                     /translation="APEPRELASAERDLAGAARVPLVGGAEALVVTGGWFADAKLAAD
                     LARVTGLAVTLVSDGRATASTAVPPPQIPADPSEPFDVEGGRRGFVVSIPDGGGVLLL
                     AAAARPVPSLPLWIVAGVALFLAVLLGWLLANVIARPLDRLAEGARAVAAGNLDARIE
                     GTGRVDVGYLADAFNTMTANIRSYVEELKDSRDELRQTLERLGATLQSTHDLQGILEV
                     VLDTSAVTLRASAGTIFLATEGEGELRLKVARGYEAPTEAVLPPGRGIAGRAAGGDPV
                     LVAGGDVDVVSAIEPQADTAIAVPLIRWDRVLGVIALYGRSVPDPFRKHDVDTLASFA
                     AQTSVAIENVLLHEEAERLSITDGLTGIFNRRYLQLSLRREIERAQRFGRSVSLLMLD
                     LDRFKDVNDEHGHQRGDEVLVELARRVMGEIRTQIDQFARYGGEEFVILLPETPCDGA
                     LVVAEKIRKAVAARPFTSQTGPPVRVTVSIGVAAYPDDGESADDLLRTADAALYRAKE
                     RGRNRVVPSVRASERA"
BASE COUNT          319 a          845 c          837 g          321 t
ORIGIN      
        1 gcacctcgcc gcccgcatcg ggcggcgtcg cgcggatcgc gtcgaagatc tcgggcgtga
       61 gcacgtacct cccgatcgcc gcgaggttcg acggcgcgtc ctccggcgcc ggcttctcga
      121 cgatgtcgac gacgcgggcg aggttgtcct cgacgaactc gggatggacc gcgccgtaga
      181 gccggatgtc ctcgcgtggg acctcctgga ccgcgatcac gctccggccg tagcgctcgt
      241 agatgtccac catgcgcccg agcagcggat ccgcgctcag cacgatgtcg tcgccgagga
      301 gcaccgcgaa cggctccgcc ccgacgtggg gctcggccgt tgcgacggca tcgccgagcc
      361 ccgccggatc gcgctggcgg atgtactgga cggtcgccat ctcggtgatc tcgcggaccg
      421 ccttgagctc gtcgtgcttg ccggaccgct cgaggtagtg ctcgagctcg aaggagcggt
      481 cgaagtggtc ctccagcgtt cgcttcccgc ggcccgtgac gatcaggatg tcggtgatgt
      541 cggcgcggac ggcctcctcg atcacgtact ggaccgccgg cttgtcgacg accggcagca
      601 tctccttcgg ctgcgccttc gtcgcgggga ggaaccgggt gccgagcccg gccgccggga
      661 cgaccgcctt cctgaccttc aatgcgatct cctcgtcgag aggagccctg agtgtatgcc
      721 ggggtcgcgt cgggcgaaac gggcgctcag gcgcgctctg aggcgcgcac cgacgggacg
      781 acccggttcc gtccccgctc cttcgcccgg tacagggcgg cgtcggccgt gcgcaggaga
      841 tcgtccgccg actcgccgtc gtccggatac gcggcgaccc cgatgctgac cgtcacccgc
      901 accggaggcc cggtctggct tgtgaacgga cgggcggcca ccgccttccg gatcttctcg
      961 gcgacgacga gggcgccgtc gcacggcgtc tccggcagca ggatcacgaa ctcctcgccg
     1021 ccgtagcggg cgaactggtc gatctgcgtc cggatctctc ccatcacccg gcgcgcgagc
     1081 tcgacgagga cctcgtcgcc gcgctggtgg ccgtgctcgt cgttcacgtc cttgaaccgg
     1141 tcgagatcga gcatcagcag cgagacggag cgcccgaacc gctgcgcgcg ctcgatctcc
     1201 cggcggagag agagctggag gtagcggcgg ttgaagatcc cggtgagccc gtcggtgatc
     1261 gagagccgct cggcctcctc gtgtagcagc acgttctcga tcgcgaccga ggtctgcgcg
     1321 gcgaacgagg cgagcgtgtc gacgtcgtgc ttgcggaacg ggtccgggac cgaccggccg
     1381 tagagcgcga tgacgccgag cacgcgatcc cagcggatca gcgggacggc gatcgcggtg
     1441 tccgcctgcg gctcgatcgc cgagaccacg tcgacgtcgc cgcccgccac gaggacgggg
     1501 tcaccgccgg ccgcccgtcc ggcgatgccg cgcccgggtg gaagcacggc ctccgtcggc
     1561 gcctcgtagc cgcgcgcgac cttgagccgc agctcgccct cgccctccgt cgccaggaag
     1621 atcgtgccgg ccgacgcccg cagcgtcacg gcggaggtat cgaggacgac ctcgaggatg
     1681 ccctggaggt cgtgggtcga ctgcagcgtc gcgccgaggc gttcgagcgt ctggcggagc
     1741 tcgtccctcg agtccttcag ctcctcgacg tacgagcgga tgttcgccgt catcgtgttg
     1801 aacgcgtcgg cgaggtatcc gacatcgacc cgccccgtgc cctcgatccg cgcgtcgagg
     1861 ttcccggccg cgacggcgcg cgcgccctcc gcgaggcggt cgagggggcg cgcgatcaca
     1921 ttcgcgagga gccagccgag cagcacggcg aggaagagcg ccacgcccgc gacgatccag
     1981 agcggaagcg acggcaccgg gcgtgccgcc gcagcgagga gcagcacgcc gcccccgtcg
     2041 gggatcgaga cgacgaaccc ccgccggccg ccctcgacat cgaacggctc ggaggggtcc
     2101 gcggggatct gcggcggcgg gaccgccgtc gaggcggtcg ctcgcccgtc ggacacgagc
     2161 gtgaccgcga gcccggtgac gcgcgcgaga tcggcggcaa gcttcgcgtc ggcgaaccac
     2221 ccgcccgtga cgacgagggc ctcggcgcct cctaccagcg ggacccgcgc cgcccccgcg
     2281 aggtcgcgct ccgccgaggc gagctcgcgc ggctccggcg cg
//
LOCUS       DASZOB010000024         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_111369, whole genome shotgun sequence.
ACCESSION   DASZOB010000024 DASZOB010000000
VERSION     DASZOB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111369"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..738)
                     /gene="thiE"
                     /locus_tag="VG709_00385"
     CDS             complement(103..738)
                     /gene="thiE"
                     /locus_tag="VG709_00385"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWC31567.1"
                     /translation="MKPERRRARLQDGRLYVVSGPREERGDLTEFLDAILGAGVDVIQ
                     LREKEAEPRHVMRWSETFREAAERHDALFVLNDRPDIAMAVGADGVHLGQNDVPVDVA
                     RRILGEHGIIGVSTRTPEQYSAVSADADYVCAGPVWETPTKPGRPAAGLEPVRYVAAR
                     ERRPWFAIGGVAEANVAEVVASGASRIVVVRAVTEADDPGVAVKALLDALP"
     gene            complement(735..935)
                     /gene="thiS"
                     /locus_tag="VG709_00390"
     CDS             complement(735..935)
                     /gene="thiS"
                     /locus_tag="VG709_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWC31568.1"
                     /translation="MRLVANGQEFDVDGAVSVAEFIRRRDLDPRYVVVERNGEPLERS
                     RYDQTLLEDGDRLELVRAVAGG"
     gene            complement(935..>1986)
                     /gene="thiH"
                     /locus_tag="VG709_00395"
     CDS             complement(935..>1986)
                     /gene="thiH"
                     /locus_tag="VG709_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HWC31569.1"
                     /translation="ADRLERTLEDFAALLSPRGSERLEELAAASHRLTAARFGRTMHM
                     YAPLYLSNECLTTCGYCGFAKNLPVARKTLSERDAVEEARHLLRQGFRSILLLTGEHE
                     RLTGVEFLERHVRALAEEVPSLAIEVQVWSEEEYRRLAEAGCEGVVIYQETYDPKRYL
                     EVHLAGRKRNYRWRLLGPERAARAGMRRIGVGALLGLHDDWRFEALATAAHARFLMRR
                     YWRSRVTVSVPRMRPSASGFQPRTLISDRELVQLVCALRLLLPDAGIVLSSREEASFR
                     DGLHRIGITHTSAGSHTEPGGYERPGEAQEQFEVADLRSPAEVAASLRAIGYEPVWED
                     PSLPGGVERGLVRAS"
BASE COUNT          279 a          738 c          698 g          271 t
ORIGIN      
        1 ccacgaacgg gtcctggaga tgggcccgca cgcgagccgg gatctcgctt cgacggatcg
       61 gtcgcagcac gacgacgtcg ccggcgatgc gcagccggcg cgtcacggca gcgcgtcgag
      121 gagcgccttg accgcgacgc cgggatcgtc ggcttcggtg acggcgcgga cgaccacgat
      181 gcgcgatgcg cctgaggcga ctacctcggc gacgttcgcc tcggcgacgc cgccgatcgc
      241 gaaccacggg cggcgctcgc gcgcggcgac gtaccgcacg ggctcgagcc cggcggcggg
      301 gcgtcccggc ttcgtcggcg tctcccacac cggtcctgcg cagacgtagt cggcgtcggc
      361 cgacaccgcg gagtattgct ccggcgtcct cgtcgagacg ccgatgatcc cgtgctcgcc
      421 gaggatgcgg cgcgcgacgt cgaccggcac gtcgttctgc ccgaggtgga cgccatcggc
      481 gccgactgcc atcgcgatgt cgggtcggtc gttgaggacg aacagcgcgt cgtggcgctc
      541 ggcggcctca cggaacgtct cgctccaccg catgacgtgg cgcggctccg cctccttctc
      601 gcggagctgg atcacgtcga caccggcgcc gaggatcgcg tcgaggaact ccgttagatc
      661 gccgcgttcc tcgcgggggc ccgagacgac gtagaggcgc ccatcctgaa gtctggcccg
      721 acgccgctcc ggcttcatcc gccggcgacg gcgcggacga gctcgaggcg gtcgccgtcc
      781 tcgagcaggg tctggtcgta gcgggagcgt tcgagcggct cgccgttgcg ctcgacgacg
      841 acgtaccggg ggtccaggtc ccggcgacgg atgaactccg cgacgctcac cgccccgtcg
      901 acgtcgaact cctggccgtt ggcgaccaag cgcatcacga cgcgcgcacg agcccgcgct
      961 cgacgccccc cgggagcgac gggtcctccc agacaggctc gtacccgatc gcgcgcagcg
     1021 atgccgcgac ctccgcgggc gaccgaaggt ccgcgacctc gaactgctcc tgggcctcgc
     1081 cgggtcgctc atagcccccc ggctccgtgt gcgaccccgc gctcgtgtgg gtgatgccga
     1141 tccggtggag gccgtcgcgg aacgacgcct cctctctcga cgacagcacg atcccggcat
     1201 ccgggagcag cagccggagc gcacacacga gctggacgag ctctctgtcc gagatcaggg
     1261 ttcgcggctg gaagccgctc gcgctcggcc gcatccgggg caccgacacg gtgacccggc
     1321 tccgccagta tcgcctcatg aggaagcggg cgtgggccgc ggtggccagc gcctcgaacc
     1381 gccagtcgtc gtggagcccg aggagcgcgc cgaccccgat ccggcgcatc ccggcgcgcg
     1441 cagcgcgctc gggtccgagg agccgccagc ggtagttccg cttccggccg gcgaggtgca
     1501 cctcgagata ccgcttcggg tcgtacgtct cctggtagat cacgacgccc tcacaccccg
     1561 cctcagcgag ccgccggtac tcctcctcgc tccagacctg cacctcgatc gcgagcgagg
     1621 ggacctcctc ggcgagcgcg cggacgtgcc gctcgaggaa ctcgacgccg gtcagccgct
     1681 cgtgctcgcc ggtcagcagc aggatcgacc tgaagccctg acggaggagg tgacgggcct
     1741 cctcgaccgc gtcgcgctcc gatagcgtct tccgcgcgac cggcaggttc ttcgcgaagc
     1801 cgcagtaccc gcacgtcgtc agacactcgt tcgagaggta gagcggcgcg tacatgtgca
     1861 tcgtccgccc gaagcgtgcc gccgtgaggc ggtgggacgc agccgcgagc tcctcgagcc
     1921 gctcggaccc gcgcggcgac agcaacgccg cgaagtcctc gagcgtgcgc tcgagccggt
     1981 cggctg
//
LOCUS       DASZOB010000025         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_61294, whole genome shotgun sequence.
ACCESSION   DASZOB010000025 DASZOB010000000
VERSION     DASZOB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61294"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1544
                     /locus_tag="VG709_00400"
     CDS             99..1544
                     /locus_tag="VG709_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWC31570.1"
                     /translation="MSVTVDRRANFVGGEWVEAIEGGVQEVLNPATGETIAEVPRGSE
                     ADVDRAVEAAKAALPEWLETTPGERAEMLLALADALEEHASDLARTEIANVGKPQSVM
                     ADEVPVSADNLRFFAGAARVVEGKAAGEYMRGYTSMIRREPVGVVGQIAPWNYPLMMA
                     VWKLGPALAAGNVVVLKPSEQTPLTTLMLAEYAAEIFPAGVLNVITGDGEPVGAGIVR
                     HPDVRMVSLTGDVATGKEVARAAADTLKRVHLELGGKAPVIVFDDADPAAVAEGIKIA
                     GYWNSGQDCTAASRVVAGPEVYDALLEELVPAVESLHVGDPAEGADVEMGPVLSKAQQ
                     ERVFGFLERAAPGGQARVLTGGDSNGERGFFVKPTVVVDVDQDDEIVKREVFGPVVTV
                     QRFAEDDQAIAWANDVDYGLSASVWTRDIGRALNAARKLEFGTVWINDHIPLASEMPH
                     GGFKQSGYGKDLSAYALEDYTRVKHVMAKID"
     gene            1630..>2321
                     /locus_tag="VG709_00405"
     CDS             1630..>2321
                     /locus_tag="VG709_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HWC31571.1"
                     /translation="MTKRFDDVVAVDAVSLEIAQGSFFALLGPSGCGKTTTLRMIGGF
                     EEPTSGTILLGDRDVTGLPPYRRDVNTVFQSYALFPHLSIFDNVAFGLRRKGVRGDSL
                     RDRGGEALELVALSGLEKRKPRQLSGGQQQRVALARALVNRPRVLLLDEPLGALDMKL
                     RKQMQLELKRIQHEVGITFVHVTHDQEEAMTMADRIAVMSEGRIEQLGAPHELYERPE
                     TAFVAGFLGVSNL"
BASE COUNT          356 a          810 c          846 g          309 t
ORIGIN      
        1 ctctggaagc aggtctacga ctggggtcgg aggctcgacg gtgcgaccaa cggcgccggc
       61 gcggccgagg acggctccgc aaccgatggg aggagcacat gagcgtgacc gttgaccgcc
      121 gcgcgaactt cgtcggcggc gagtgggtgg aggcgatcga gggcggcgtc caggaggtcc
      181 tgaacccggc caccggcgag acgatcgccg aggtgccgcg cgggagcgag gcggacgtcg
      241 accgcgccgt cgaggccgcg aaggcggcgc tcccggagtg gctcgagacg acgccgggcg
      301 agcgcgccga gatgctcctc gcgctggccg acgcgctcga ggagcacgcg tccgacctcg
      361 cccgcaccga gatcgcgaac gtcgggaagc cgcagtcggt catggccgac gaggtgccgg
      421 tctcggccga caacctccgc ttcttcgcgg gcgcggcgcg cgtcgtcgag ggaaaggcgg
      481 ccggcgagta catgcgcggc tacacctcga tgatccggcg ggagcccgtg ggcgtcgtcg
      541 ggcagatcgc gccctggaac tacccgctga tgatggcggt gtggaagctc ggtcccgcgc
      601 ttgccgccgg aaacgtcgtc gtcctcaagc cgtcggagca gacgccgctg acgacgctca
      661 tgctggccga gtacgcggcg gagatcttcc ccgccggcgt cctcaacgtc atcacgggcg
      721 acggcgagcc ggtgggcgcc ggcatcgtgc gccacccgga cgtccgcatg gtctcgctga
      781 ccggcgacgt cgcgaccggc aaggaggtcg cccgcgcagc ggccgacacg ctgaagcgcg
      841 tccacctcga gctcggcggc aaggcgccgg tgatcgtctt cgacgacgcc gaccccgccg
      901 ccgtcgccga gggaatcaag atcgccggct actggaactc gggccaggac tgcacggccg
      961 cctcgcgcgt cgtcgcgggc cccgaggtgt acgacgccct gctcgaggag ctcgtcccgg
     1021 cggtcgagtc gctccacgtc ggcgaccccg ccgagggggc cgacgtcgaa atggggccgg
     1081 tcctctcgaa ggcgcagcag gagcgcgtgt tcggcttcct cgagcgggcg gcgcccgggg
     1141 gacaggcgcg cgtcctcacg ggcggcgact cgaacggcga gcgcggcttc ttcgtcaagc
     1201 cgacggtggt cgtcgacgtc gaccaggacg acgagatcgt gaagcgtgag gtgttcggcc
     1261 cggtcgtgac ggtgcagcgc ttcgccgagg acgaccaggc gatcgcgtgg gcgaacgacg
     1321 tcgactacgg cctctccgca tccgtgtgga cgcgcgacat cgggcgggcg ctgaacgcgg
     1381 cgcgcaagct cgagttcgga acggtctgga tcaacgacca catcccgctg gcgtccgaga
     1441 tgccgcacgg cggcttcaag cagtcgggct acggcaagga cctgtccgcc tacgcgctcg
     1501 aggactacac gcgggtcaag cacgtcatgg caaagatcga ctaagcctgc cgtggcggtc
     1561 cgagagcatg cggcggctcg gccgggcgcg gaggcggcgg tgcctcccgc ggacgtcacc
     1621 ctgcgcgacg tgacgaagcg gttcgacgac gtcgtcgccg tcgacgccgt gtcgctcgag
     1681 atcgcgcagg gcagcttctt cgccctcctc ggcccgtcgg ggtgcggcaa gaccacgacg
     1741 ctccgcatga tcggcgggtt cgaggagccg acgtccggca cgatcctgct cggcgaccgc
     1801 gacgtcaccg gcctcccgcc ctaccggcgc gacgtgaaca ccgtcttcca gagctacgcg
     1861 ctcttcccgc acctctcgat cttcgacaac gtcgccttcg gactccgccg aaagggagtc
     1921 cgcggcgatt cgcttcggga ccgcggcggc gaggcgctcg agctcgtcgc gctctcgggg
     1981 ctcgagaagc gcaagccgcg ccaactatcc ggggggcagc agcagcgcgt cgcgctcgcg
     2041 cgcgcgctcg tcaaccgtcc gcgcgtcctc ctcctcgacg agccgctcgg ggcgctcgac
     2101 atgaagctcc gcaagcagat gcagctcgag ctgaagcgga tccagcacga ggtcgggatc
     2161 acattcgtgc acgtcacgca cgaccaggag gaagcgatga cgatggccga ccggatcgcc
     2221 gtcatgagcg aggggcggat cgagcagctc ggggcgccgc acgagctcta cgagcgtccc
     2281 gagacggcct tcgtcgccgg cttcctcggc gtctccaacc t
//
LOCUS       DASZOB010000026         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_171952, whole genome shotgun sequence.
ACCESSION   DASZOB010000026 DASZOB010000000
VERSION     DASZOB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171952"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VG709_00410"
     CDS             <1..1247
                     /locus_tag="VG709_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC31572.1"
                     /translation="IGKTRAGAVGAGLGSHDEREQTLNQLLVEMDGFDASKGVIIMAA
                     TNRPDVLDPALLRPGRFDRQVIVDRPDLRGREAILTVHARGVALADGVDLSVIAARTP
                     GFTGADLANVVNEAALLAARREKNAVGTEELEEAIDRVVAGLERKSRVMSEKEREIVA
                     FHEMGHALVALSVKNADPVHRVTIIPRGAAALGMTMQRPLEDRYLMTKPELEDRLAIL
                     LGGRTAEEIAFKQISTGAQNDLERATEIARSMVVEFGMSERLGPLSYGRDGMRGGDGR
                     PLFPTFDRPEMSDDRARLVDEEVSRILGEAHGRARKIIQSKRQLLTKIGRLLMVREVL
                     EGKDLRAYVDGEAVVPDAEEEARRRDKEHAAQQRKAKQEARRKEKEAADRAAEERTMR
                     PLSPDEDALPNGETEEIPRRIG"
     gene            1253..1693
                     /locus_tag="VG709_00415"
     CDS             1253..1693
                     /locus_tag="VG709_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWC31573.1"
                     /translation="MSLRRSSAALVVLVALFVASCGGEADTSGSASKGTPQAKGETPQ
                     EKPTGGDRVEVEVEAEDFAFEPNTVTVGAGAAIALKFNNRDEGVPHTFTVYETDAAEQ
                     EIFDTGNLTGDAEETYRFTAPSESGSYFFRCDVHPEMTGTFVVQ"
     gene            complement(1709..2713)
                     /locus_tag="VG709_00420"
     CDS             complement(1709..2713)
                     /locus_tag="VG709_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWC31574.1"
                     /translation="MPEIGISIGGPMPTIADLARMAEEGGYESVWVAETAYSAYVQAA
                     LACQATSRITVGTNIALAVPRSPTITAMTARDLSELSGGRFVLGLGSQVKRVNEHRFS
                     VPFEHPAPKMAEYVEVVRKVLATFRGEAIEHRGRFYTVTMPPFPGAAAAPDVPIYLAA
                     VNEKMCEAAGRAADGMLGHPMTSPKYIAEVVRPAVERGATAAGRDPSDISITNSVILQ
                     MNDDRDRARFEAALQIGFYATTRTYAPVLALHGFEDSVAPLRDAYRSFDLAKMAELAL
                     PMVDTYAITGTEDECREQLSQFDGLVDRVLLGGAWVGPSQEAIAENYRRILQTFRPAG
                     "
     gene            2803..3030
                     /locus_tag="VG709_00425"
                     /pseudo
     CDS             2803..3030
                     /locus_tag="VG709_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3117..>3331
                     /locus_tag="VG709_00430"
     CDS             3117..>3331
                     /locus_tag="VG709_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31575.1"
                     /translation="MTESTDRTITGGVRAADLAEVAREVGTVATVYLATEPDVENAGQ
                     RSELRWKNARTALADAGAPEEALTAIGS"
BASE COUNT          533 a         1137 c         1221 g          440 t
ORIGIN      
        1 cgatcggcaa gacgcgcgcc ggtgcggtgg gcgcggggct cggctcacac gacgagcgcg
       61 agcaaacgct caaccagctc ctcgtcgaga tggacgggtt cgacgcctcc aagggcgtca
      121 tcatcatggc ggcgaccaac cgccccgacg tcctcgaccc ggcgctgctg cggcccgggc
      181 ggttcgaccg tcaggtcatc gtcgaccgcc cggatctccg cggccgcgag gcgatcctca
      241 cggtgcacgc gcgtggcgtt gcgctcgcag acggcgtcga cctgtcggtg atcgccgctc
      301 ggacccccgg gttcacgggc gccgatctcg cgaacgtggt caacgaggcc gcgctcctcg
      361 cggcgcggcg cgagaagaac gccgtcggga ccgaggagct cgaggaggcc atcgaccgcg
      421 tcgtcgcggg tctcgagcgc aagagccgcg tcatgagcga gaaggagcgc gagatcgtcg
      481 cgttccatga gatgggccat gccctggtcg cgctctcggt caagaacgcc gacccggtcc
      541 accgcgtcac gatcatcccg cgcggcgcgg cggccctcgg catgacgatg cagcgtcctc
      601 tcgaggaccg ctatctcatg acgaagccgg agctcgagga ccggctcgcg atcctgctcg
      661 gcgggcgcac cgcggaggag atcgcgttca agcagatctc gaccggcgcg cagaacgacc
      721 tcgagcgcgc caccgaaatc gcgcgatcca tggtcgtcga gttcgggatg agcgagcgcc
      781 tcggcccgct gtcgtacgga cgcgacggta tgcgcggcgg ggacggtcgc ccgctgttcc
      841 cgacgttcga tcgtccggag atgtccgacg acagggcgcg gctcgtcgac gaggaggtct
      901 cgcgcatcct cggcgaggcc cacggccgcg cgcgcaagat catccagtcg aagcggcagc
      961 tgctcacgaa gatcgggcga ctgctgatgg tgcgggaggt cctcgagggc aaggacctca
     1021 gggcatacgt cgacggcgag gcggtcgtcc ccgacgccga ggaggaggcg cgcaggcgcg
     1081 acaaggaaca cgcggcccag cagcgcaagg cgaagcagga ggccaggcgg aaagagaagg
     1141 aggcggcgga ccgggcggcg gaggagcgga cgatgcgacc gctgtccccg gacgaggacg
     1201 ccctgccgaa cggcgagacc gaagagatcc cgcggaggat cgggtgagca cgatgtcctt
     1261 gagacgaagc tccgcggcac tggtggtcct cgtggcgctg ttcgtcgcct cgtgcggcgg
     1321 cgaggccgac acgtcgggat cggcctcgaa gggaacgccg caggccaagg gcgagacgcc
     1381 tcaggagaag ccgacgggcg gtgaccgggt cgaggtcgag gtcgaggccg aggacttcgc
     1441 cttcgagccg aacaccgtca cggtcggagc gggcgcggcg atcgcgctga agttcaacaa
     1501 ccgcgacgaa ggcgtgccgc acacgttcac ggtgtacgag accgacgcgg cggagcagga
     1561 gatcttcgac acggggaacc tcaccggcga cgccgaggag acctaccgct tcacggcgcc
     1621 gtccgagtcg ggcagctact tcttccggtg cgacgtccac cccgagatga ccggcacctt
     1681 cgtcgtccaa taggacgcga cgcggtcgct agccggccgg cctgaaggtc tgcaggatcc
     1741 ggcggtagtt ctcggcgatc gcctcctgcg acgggcccac ccacgccccg ccgagcagga
     1801 cgcggtccac gaggccgtcg aactgcgaga gctgctcccg gcactcgtcc tccgtgccgg
     1861 tgatcgcgta ggtgtcgacc atcggcagcg cgagctccgc catcttcgcc aggtcgaacg
     1921 agcggtatgc gtcccgtagc ggcgccacgc tgtcctcgaa cccgtggagc gcgagcacgg
     1981 gcgcatacgt gcgcgtggtc gcgtagaacc cgatctggag cgcggcctcg aacctcgcgc
     2041 ggtcgcgatc gtcgttcatc tggaggatca cgctgttcgt gatgctgatg tccgagggat
     2101 cgcgtccggc ggccgtggcg cctcgctcga cggccggccg cacgacctcg gcgatgtact
     2161 tcggcgacgt catcgggtgt ccgagcatcc cgtcggcggc ccgcccggcc gcctcgcaca
     2221 tcttctcgtt gaccgccgcg aggtagatcg ggacgtcggg cgccgctgcg gcgccgggga
     2281 acggaggcat cgtgaccgtg tagaagcggc cgcggtgttc gatcgcctcg ccgcggaacg
     2341 tcgccaggac cttgcggacg acctccacgt attccgccat cttgggggcg gggtgctcga
     2401 acggcaccga gaaccggtgc tcgttgaccc gcttcacctg ggagccgagt cccagcacga
     2461 accgcccgcc ggagagctcc gagaggtcgc gcgcggtcat cgcggtgatc gtcgggctcc
     2521 gcggaaccgc cagcgcgatg ttcgtcccga ccgtgatgcg tgaggtcgcc tggcacgcga
     2581 gcgccgcctg cacgtacgcc gagtaggccg tctccgcgac ccagacgctc tcgtagccgc
     2641 cttcctccgc catgcgcgcg aggtccgcga tggtcggcat cggcccgccg atcgagatcc
     2701 cgatctccgg cacgcgcgcc agcgtagccg gggggctcac cgtgcggcaa cggtgtgcca
     2761 cccgcctcgc ttcggggtaa ggggaacgcg tggaggtgag ccatggcgag atgtgacgtc
     2821 tgcggcaacg actacgacaa ggcattcacg gtcgagggcc ccgccgggaa cgggacgttc
     2881 gacagcttcg agtgcgcgat ccactcgctc gcgccgacct gcggacattg cggcgtccgg
     2941 atcatcggcc acggcgtcga gtcgaacggg gcgatctact gctgcgcgca ctgcgcccgt
     3001 gaggaggggc agcgcgggct cgcggatcgc acgggcgatg gcggtggcgg cggcgaggga
     3061 cccgagggcg tcgctccgac ccggccgccc gccgagagct gaacacggag gaagccgtga
     3121 ccgagagcac cgaccgaacg atcacgggcg gcgtccgtgc cgccgacctc gccgaggtgg
     3181 cgcgcgaggt gggcaccgtc gcgaccgtgt atctcgcgac ggagcccgac gtcgagaacg
     3241 ccggccagcg gtcggagctc cgctggaaga acgcccgtac cgcgctcgcg gacgccggcg
     3301 cgccggagga ggcgctaacg gcgatcgggt c
//
LOCUS       DASZOB010000027         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_101738, whole genome shotgun sequence.
ACCESSION   DASZOB010000027 DASZOB010000000
VERSION     DASZOB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101738"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="VG709_00435"
     CDS             complement(<1..2372)
                     /locus_tag="VG709_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWC31576.1"
                     /translation="MRAIRLRSVLVVLTAIGAFVAGVVPGALAKGPPPEKARNRPPAD
                     YVAMADELSQPIYPQTIREVHTVEAFDGESLYVEIVRPDPEEYGEGPWPVVLEASPYH
                     GTLHARTGTRILPDPVDEDGQQVGLTGFFAPRGYAVAMMDLRGTGRSTGCLEHLGPDD
                     SRDLSTVIEWLAGQEWSNGRVGMTGHSYVGSTPNVAAALPKEDREGLVTIVPSAGLAS
                     MYDHQFSKGVPWNLQWAGPQWAYEILAIDRDLPPQLGSDPVQGGATGDNWENAPNPQF
                     GCGLQHSALTAGTGQVTGQYQGWHAARDWRTAAAATDIPIFMVHGVNDNAARIPAAEW
                     FFADRPWQPGDKIWLGQWDHGSGNHRCGSTQNPPQRVAHPNCRFDQWTYALLAWFDSH
                     LKQMDVDTGPAVEVFLNEQRPVNVTQVVDPESLDARVFTSNRWSFPRRALELFPDATD
                     MSLKFAPPADNASASFRTVADAIAAHVAAGNVTFTSDPLPERTLITGMPDLQLNASVV
                     SPQVAHLVTTLWRVRTYENEKGETVTEREQMNFCAINPLQRNGIDRVEPVTPNQEMEL
                     DPQCFTMAHWVPAGQRLQLVVSTTTTPAHYATSGTYPQVTIFTGPEKTRYTLPSVVKP
                     KLYEDVPLRIRTEQPPVGPAQPAITGSVTLGPPGVPPGAREPGLDSQFFEFDILDGFD
                     NASFTVAANAAVPAGDIDLYLQRQRADGTWTGALASGTDFVRAGNPVEDIESGRRMPG
                     HYRVEVVNFAAPPGPVNLTITFFNQAGEPGPAPGSGSEAYQLVEGNQAAVG"
     gene            2562..>3133
                     /locus_tag="VG709_00440"
     CDS             2562..>3133
                     /locus_tag="VG709_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HWC31577.1"
                     /translation="MDAAAVVREVQERRVPLVRLWFTDVLGFLKSFAVPAEELERALS
                     DGIGFDGSAIEGFARVEEEDVIARPDPVTFRLLPARGGDVAARMLCDITYPDGAPFEG
                     DPRFVLRRNLERARALGYRFVAAPEIEFFLSRSAEQFDPADRGGYFDLTASDVAEAFR
                     RRTLDALGALGIAVESVHHEDAPGQQEIDLA"
BASE COUNT          427 a         1086 c         1101 g          519 t
ORIGIN      
        1 ccgaccgccg cctggttccc ctccacgagc tggtacgcct ccgaaccgga gcccggcgcc
       61 ggacccggct cccccgcttg gttgaagaac gtgatcgtca gattcacggg gcccggggga
      121 gcggcgaagt tcacgacctc gacgcggtag tgccccggca tccttcgacc ggactcgatg
      181 tcctcgaccg gatttccggc gcggacgaag tccgtcccgc tcgccagagc gcctgtccac
      241 gtcccatcgg cacgctgccg ctggaggtag aggtcgatgt cgccggcggg aaccgccgcg
      301 ttggccgcga ccgtgaacga cgcgttgtcg aacccgtcga ggatgtcgaa ctcgaagaac
      361 tggctgtcca gccccggctc gcgagcgccg ggcgggacac cgggcggccc gagcgtgacg
      421 gatcccgtaa tcgcgggctg cgccgggcca acgggcggct gctccgtccg gatccgcagc
      481 ggcacgtcct cgtagagctt cggcttcacc accgacggca gggtgtaccg cgtcttctcc
      541 ggccccgtga agatcgtcac ctgcgggtac gtcccggagg tcgcgtagtg ggccggcgtc
      601 gtcgtcgtgc tcacaacgag ctggagccgc tgccccgcgg ggacccagtg cgccatcgtg
      661 aagcactgcg ggtccagctc catctcctgg ttcggcgtca ccggctcgac cctgtcgatc
      721 ccgttccgct ggagcgggtt gatcgcgcag aagttcatct gctcgcgctc ggtgaccgtc
      781 tcacccttct cgttctcgta ggtcctgacg cgccacagcg tcgtcacgag gtgcgcgacc
      841 tgcgggctca cgaccgacgc gttgagctgc aggtccggca tgcccgtgat cagcgtccgc
      901 tccgggagag ggtccgacgt gaacgtaacg ttgccggccg cgacgtgcgc cgcgatggcg
      961 tcggcgaccg tcctgaagct cgcgctcgcg ttgtccgcgg gcggcgcgaa cttgagcgac
     1021 atgtcggtcg cgtcggggaa gagctccaac gcccgtcggg ggaagctcca ccggttcgac
     1081 gtgaataccc tggcgtcgag cgactcgggg tcgacgacct gcgtcacgtt gacgggccgc
     1141 tgctcgttga ggaaaacctc gacggcggga cccgtgtcca cgtccatctg cttgaggtgg
     1201 ctgtcgaacc acgccagcag cgcgtacgtc cactggtcga accggcagtt cgggtgcgcg
     1261 acacgctgcg gcgggttctg cgtgctgccg caacggtggt ttccggaccc gtggtcccac
     1321 tgtccgagcc agatcttgtc gccgggctgc cagggtcggt ccgcgaagaa ccactcggcg
     1381 gccgggatgc gggccgcgtt gtcgttgacg ccgtggacca tgaagatcgg gatgtcggtc
     1441 gccgcggcgg cggtgcgcca gtcgcgtgcg gcgtgccagc cctggtactg acccgtgacc
     1501 tggcccgtcc cggcggtcag cgccgagtgc tggagcccgc atccgaactg tgggttcgga
     1561 gcgttctccc agttgtcgcc ggttgcgccg ccctggacgg gatccgagcc gagctgtggc
     1621 ggcaggtcgc ggtcgatcgc gaggatctcg tacgcccact ggggaccggc ccactggagg
     1681 ttccacggga cccccttgct gaactggtgg tcgtacatcg acgcgaggcc cgcgctcggc
     1741 acgatcgtga ccagcccctc gcgatcctcc ttcggaagcg cggccgcgac gttcggcgtc
     1801 gatccgacat acgagtggcc ggtcatgccg acgcgcccgt tcgaccactc ctgtccggca
     1861 agccactcga tgacggtgct caggtcccta gagtcgtccg gcccaaggtg ctcgaggcat
     1921 ccagtggagc gaccggtgcc gcggagatcc atcatcgcga cggcgtaccc gcgcggcgcg
     1981 aagaaccccg tgaggccgac ctgctggcca tcctcgtcga cggggtcggg gaggatacgg
     2041 gtccccgtcc gggcatggag cgtgccgtga tacgggctcg cctcgaggac caccggccat
     2101 gggccttcgc cgtactcctc gggatcggga cggacgatct cgacgtagag gctctcgccg
     2161 tcgaacgcct cgacggtgtg gacctcgcgg atcgtctgcg gataaatggg ctgtgagagc
     2221 tcgtcggcca tcgcgacgta gtcggccggc gggcggttcc gcgccttctc gggcggcgga
     2281 cccttcgcga gggcgccggg gaccaccccg gccacgaagg cgccgatcgc ggtcagtacg
     2341 accagcacgg aacggagacg tatcgctcgc attgctccct cctctttggg ggcggttcga
     2401 cggggcatcc ggggacccgg acgcggtaga cgttcgacgc gcccgctccc ggttccttcc
     2461 gaagtattca acatggttgg gtggtttgtc catttcgaga cggcatgtcg ggtccagggg
     2521 cctccgctgg acggctctcc gcacgggcta ccatccgctc gatggacgcg gcagcggtcg
     2581 tccgcgaggt ccaggagcgc cgcgtgcccc tcgtccggct gtggttcacc gacgtgctcg
     2641 ggttcctcaa gtcgttcgcg gtccccgctg aggagctcga acgagcgtta tcggacggca
     2701 tcggattcga cgggtcggcg atcgaggggt tcgcgcgggt cgaggaggag gacgtgatcg
     2761 cccggccgga ccccgtgacg ttccggctgc tcccggcgcg cggcggtgac gtcgccgccc
     2821 gcatgctgtg cgacatcacg tatccggacg gcgcgccgtt cgagggcgac cctcggttcg
     2881 tgttgcggcg gaacctcgag cgcgcgcggg cgctcggata ccggttcgtc gccgcgcccg
     2941 agatcgagtt cttcctgtca cggtccgccg agcagttcga ccccgccgac cgaggcggct
     3001 acttcgacct caccgcctcc gacgtcgccg aggcgttccg ccgccgcacg ctggacgcgc
     3061 tcggcgcgct cggcatcgcg gtcgagagcg tccaccacga ggatgcgccc ggccagcagg
     3121 agatcgacct cgc
//
LOCUS       DASZOB010000028         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_171956, whole genome shotgun sequence.
ACCESSION   DASZOB010000028 DASZOB010000000
VERSION     DASZOB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171956"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1264
                     /locus_tag="VG709_00445"
     CDS             68..1264
                     /locus_tag="VG709_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWC31578.1"
                     /translation="MSDARAVGRPRTRVGIVGAGFAASSHLDALSRVGGVEVTAIAAS
                     TPERGRAAAERYGIERAREDWRAVVEDDEVDVVHNCTPNFLHAEVSSAALSAGKHVLS
                     EKPLAMDSRETAALRDHAASTQAVTGVCFNYRHYPLVQHARAMLSSGDEGDVHLVRGT
                     YLQDWLLLEDDWNWRLETSKAGTARAMGDIGSHWLDLVQHVTGDRVEAVMADLGTLHT
                     ERLRPAGEVETFARAEGEKERVAVGTDDFGSVLLRFSGGASGVFTVSQVSAGRKNHLT
                     FEIDTARASLSWNQEEPNTLRIGRRDAPSSELPRDPSLLAPEAAALARYPGGHQEGWP
                     DALRNLLEDFYAAVAAHRGGREHRSTFATFDEAHGITLVVEAIAESHRSRAWVDVAAR
                     PAIGTS"
     gene            1287..2255
                     /locus_tag="VG709_00450"
     CDS             1287..2255
                     /locus_tag="VG709_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWC31579.1"
                     /translation="MKLGVFLVLYSQLPFEEALDKVKAMGAEAVELGTGNYPGDAHCK
                     PDELLGDTDAVRRFKKAVESRGLVISALSQHGNPLHPDRERAERDHEVWRKTVELAEQ
                     LEVGVVNAFSGCPGDHDGARWPNWVTCPWPEDYLEVLEWQWNRKVIPYWTEESAFADS
                     HGVSVGFEMHPGFVVYNPETLLRLREACGPRIGANFDPSHLFWQGIDPVEAIKLLGKE
                     GAIFHVHAKDTYLDRGNIVRNGVLDTKHYGEVLDRSWVFRTVGYGHGEHTWREIVSAL
                     RAVGYDHVLSIEHEDSLMSIDEGLGKAIDLLSRQILREQPAEMWWA"
     gene            complement(2260..2550)
                     /locus_tag="VG709_00455"
     CDS             complement(2260..2550)
                     /locus_tag="VG709_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31580.1"
                     /translation="MQFIGLTTLRQSITPRRLLGRVYASSNVVGEIATLLGALTGGLL
                     GDTIGLRPAIATAAVMYSIPFFYSLASPLRRASLIAEEAKAEPEGEPEPERS"
     gene            complement(2532..3551)
                     /locus_tag="VG709_00460"
     CDS             complement(2532..3551)
                     /locus_tag="VG709_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC31581.1"
                     /translation="MFRPKLSPIWQQRNFRLLWFGETISLLGSTVSHFALPVVAVVTL
                     HVSAGQMGLMRALGSAPSIVLGLLAGVWVDRLDRRRLLVATNLLAAVLMASVPIAHVV
                     GSLTVFHLYALWFAFGILGAFWWPAWNSFLPSIVPPDDLVEANSKVEFAWSSMGVAGP
                     ALGALLISWFTAPGVLAFDAATFVICAGFIVRIRPPAVTTDDGPRVSTFRQIQDGLRA
                     TFVDVMQRAITIPRVILDFVDGLSLTVFALYVLREVGLSPALLGVALALSWLGFALGA
                     AIAPRLERRLGIGTMIVLGLLLVGISPYTMVIANRGFRTGSTSCSSPRRDGSAVSEAS
                     CSSSA"
     gene            3632..>3990
                     /locus_tag="VG709_00465"
     CDS             3632..>3990
                     /locus_tag="VG709_00465"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWC31582.1"
                     /translation="MPERAHDTIGPEPEHPVERAWLYQSWNVATWLHWPYDPAAISHV
                     VPDGLELDTFEGNAYVGLVPFVIENQRLAGTPAIPWLSRARETHLRTYVTAPDGARGS
                     YFFYLENERLAAVLGGRL"
BASE COUNT          679 a         1367 c         1419 g          525 t
ORIGIN      
        1 gcacgcgcgt cggcgcgcag gtctcgatgc cgacgcgtga cgacgtcgag gctctagcat
       61 cggaagcgtg agcgacgcgc gcgcggtggg acgtccgagg acccgcgtcg ggatcgtcgg
      121 cgcggggttc gccgcctcgt cgcacctcga cgcgctctct cgggtgggcg gcgtggaggt
      181 cacggcgatc gccgccagca cccccgaacg cggacgcgcg gcggccgagc ggtacggcat
      241 cgagcgggcg cgcgaggact ggcgtgcggt cgtcgaggac gacgaggtcg acgtcgtcca
      301 caactgcacc ccgaacttcc tccacgccga ggtctcgagc gcggcgctct cggcgggcaa
      361 gcacgtgctg tcggagaagc cgctcgcgat ggactcgcga gagacggccg cgctccgcga
      421 ccacgccgcg tcgacgcagg ccgtgaccgg cgtctgcttc aattaccgcc actacccgct
      481 cgtccagcac gccagggcga tgctctcctc cggcgacgag ggggacgtgc acctcgtccg
      541 cgggacctac ctgcaggact ggctgctgct cgaggacgac tggaactggc gcctcgagac
      601 ctcgaaggcc ggcacggcgc gcgcgatggg ggacatcggc tcgcactggc tcgatctcgt
      661 ccagcacgtc accggcgatc gcgtggaagc cgtcatggcg gacctcggga cgctccacac
      721 cgagcgcctg cgccctgccg gggaggtcga gacgttcgcg cgagcggagg gcgagaagga
      781 gcgggtcgcc gtcgggaccg acgacttcgg ctcggtcctg ctgcggttct ccggcggcgc
      841 gagcggcgtc ttcacggtct cgcaggtgag cgccgggcgg aagaaccacc tcacattcga
      901 gatcgacacc gcgcgcgcgt cgctctcgtg gaaccaggag gagcccaaca cgctccggat
      961 cggccgtcgc gacgcgccga gctcggagct cccgcgcgac ccgtcgctgc tggcgccgga
     1021 ggccgccgcg ctcgcacgct atcccggcgg tcaccaggag ggctggcccg acgccctccg
     1081 gaacctctta gaggacttct acgcggcggt ggccgcgcac cgcggcggac gggagcatcg
     1141 gtcgacgttc gcgacgttcg acgaagctca cggcatcacg ctcgtcgtcg aggcgatcgc
     1201 ggagagccac cgttcgcgtg cctgggtcga cgtcgcggcc cggccggcga tcggaacgtc
     1261 gtgaaggacg gagactggag gacgacatga agctcggtgt gttcctggtc ctgtactcgc
     1321 agctgccgtt cgaggaggcg ctcgacaagg tgaaggcgat gggggcggag gccgtcgagc
     1381 tcggcaccgg caactatccc ggcgacgccc actgcaagcc cgatgagctc ctcggagaca
     1441 cggacgccgt ccggcgcttc aagaaggccg tcgagagccg cgggctcgtc atcagcgcgc
     1501 tctcccagca cgggaacccg ctccatccgg accgcgagcg cgcggagcgc gaccacgagg
     1561 tctggcggaa gacggtcgag ctcgcggagc agctcgaggt cggcgtcgtg aacgcgttct
     1621 cggggtgccc cggcgaccat gacggcgcca ggtggcccaa ctgggtcacg tgcccgtggc
     1681 ccgaggacta cctcgaggtc ctcgagtggc agtggaaccg gaaggtgatc ccgtactgga
     1741 ccgaggagtc ggccttcgcc gactcccacg gcgtgagcgt cgggttcgag atgcacccgg
     1801 ggttcgtcgt gtacaacccg gagacgctgc tccgactgag ggaggcgtgc ggaccgcgga
     1861 tcggcgcgaa cttcgacccg agccatcttt tctggcaggg catcgacccg gtggaggcga
     1921 tcaagctcct cggcaaggaa ggcgcgatct tccacgtgca cgccaaggac acgtacctcg
     1981 accgcggcaa catcgtccgg aacggcgtcc tcgacaccaa gcactacggc gaggtcctcg
     2041 accgttcgtg ggttttccgc acggtcgggt acgggcacgg cgagcacacg tggcgcgaga
     2101 tcgtcagcgc gctgagggcc gtcgggtacg accacgtgct ctcgatcgag cacgaagaca
     2161 gcctgatgtc gatcgacgag ggcctcggga aggccatcga cctcctctcg cgccagatcc
     2221 tccgcgagca gccggcagag atgtggtggg cctagcggac taggagcgct cgggctccgg
     2281 ctcgccctcc ggctcggcct tcgcctcctc cgcgatcagc gaggcgcgcc gcagcggcga
     2341 cgccagcgag tagaagaatg ggatcgagta catcaccgcc gcggtggcga tcgccggtcg
     2401 gagcccgatc gtgtcgccga gcaacccgcc ggtcagcgcg ccgagcagcg tggcgatctc
     2461 gccgaccacg ttcgagctcg cgtagacacg cccgaggagg cggcgcggcg tgatcgattg
     2521 ccggagcgtg gttaggccga tgaactgcac gatgcctccg aaaccgccga tccatcccgg
     2581 cgtggcgaag aacacgacgt tgacccagtc cggaagcccc ggttcgcgat gaccatcgtg
     2641 tagggactga tgccgacgag caggaggccg agcacgatca tcgtgccgat gccgagtcgc
     2701 cgctcgagcc tcggcgcgat cgcggcaccg agagcgaacc cgagccagct cagcgcgagc
     2761 gcgaccccca gcagcgctgg gctgagcccg acctcgcgga gcacgtagag ggcgaagacc
     2821 gtcaacgaaa gcccgtcgac gaagtcgagg atcacgcgcg ggatcgtgat ggctcgctgc
     2881 atcacatcga cgaacgtcgc tcggagcccg tcctggatct gccggaacgt actgacgcgc
     2941 ggaccgtcgt ccgtcgtgac ggccggcggg cggatccgga cgatgaaccc ggcgcagatc
     3001 acgaacgtcg cggcatcgaa ggccagtact cccggcgcgg tgaaccagga gatcagcagc
     3061 gcaccaaggg ccggcccggc gacgcccatc gacgaccacg cgaactcaac cttgctgttc
     3121 gcttcgacga ggtcgtcggg cggcacgatc gagggaagga acgagttcca cgccggccac
     3181 cagaacgcgc cgaggatccc gaacgcgaac cagagcgcgt agaggtgaaa gaccgtgagg
     3241 gagccgacga cgtgcgcgat cggaacggat gccatcagga cggcggccag caggttcgtg
     3301 gccacgagca gccgtcggcg atccaggcgg tccacccaga ccccggccag cagtccgaga
     3361 acgatgcttg gggcgcttcc gagcgcccgc atcagcccca tctgaccggc gctgacgtgc
     3421 aaggtcacga ccgccacgac cggcagcgcg aaatgggaga cggtgcttcc cagaagcgag
     3481 atcgtctccc cgaaccacag caggcggaaa ttgcgctgct gccagatcgg cgacagcttc
     3541 gggcggaaca tcgaacccct cgccgctcgg tggacgcgag ccgccagagt ctacgggttc
     3601 gcgaggcggg gagggggtat gagcggcgcg gatgccggag cgagcgcacg acacgatcgg
     3661 acccgagccg gagcatccgg tcgaacgcgc gtggctgtac cagtcgtgga acgtcgcgac
     3721 gtggctccac tggccctacg accccgcggc gatcagccac gttgtccccg acggcctcga
     3781 gctcgacacg ttcgagggga acgcctacgt cgggctcgtg cccttcgtga tcgagaacca
     3841 gcggctcgcc gggacgccgg cgatcccgtg gctctcgcgg gcgcgcgaga cgcacctacg
     3901 gacctacgtc accgcccccg acggcgcgcg cggctcgtac ttcttctatc tcgagaacga
     3961 acggctcgcg gcggtgctcg gcggccggct
//
LOCUS       DASZOB010000029         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_91646, whole genome shotgun sequence.
ACCESSION   DASZOB010000029 DASZOB010000000
VERSION     DASZOB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91646"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VG709_00470"
     CDS             <1..261
                     /locus_tag="VG709_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWC31583.1"
                     /translation="VACSPQAWAAGAPFLLLQAMLGISAVAHENLLTVNKPHLPPWLN
                     TVQLDGMRGGESRISLEFRRDGDTTSFSMTSKEGDVRVVMEE"
     gene            complement(266..1438)
                     /locus_tag="VG709_00475"
     CDS             complement(266..1438)
                     /locus_tag="VG709_00475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HWC31584.1"
                     /translation="MNVLDLVVTAVVVFSVVTGFRRGAALQLVTYTGLFIGLLAGALL
                     APVVAGFIVEPFGQAVIALTTLLLLAAIGDAIGWMIGKRVWIAARRSVLDPVDQAAGS
                     VVAGVAVLLAVWFLAFNLVQGPFPTLARQIRGSAVVRGIDTVLPRPPAVLSQVRQFLN
                     RFGFPDVFADLPPAPAGPVQGPSRGEVGRAFRAADESTVKIVGEACGRIQEGSGFVVE
                     PNYVVTNAHVVAGVDAPQVQQQGGATFDATTVLFDDDLDLAVLRVGSAPGPDLDLLQG
                     VLDRGAVGAVLGYPRGGNLQGDPAAVRQTLSPLGYDIYNDDTVRREVYELQAPVQPGN
                     SGGPFVTPDGQVAGVVFAASTTNDGVGYALTSEEVLPRVRQALGSTREVDTGPCIR"
     gene            1495..2292
                     /locus_tag="VG709_00480"
     CDS             1495..2292
                     /locus_tag="VG709_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWC31585.1"
                     /translation="MTITIAHISDPHVGSPYFVPNLMNRVIVELNELEPDAVICTGDF
                     TNFGYRQEFKTAVAYLDRIRAPVYSVPGNHDARNVGYVHFEELIGPRHWAVDIRGVRI
                     VGADSSEPDLDHGQIGRERYEWLREQFSVPADLKVFAIHHHLLPVPGTGRERNIVYDA
                     GDLLEVLIDAGTHVVLTGHKHVPYVWRLENLYVANAGTCASLRLRGHTKPCYNVIEVE
                     GDEVKIFRRFPFGGSTLNAHFSITSGTQYHRELENVISDGSTVLPLD"
     gene            2321..>2600
                     /locus_tag="VG709_00485"
     CDS             2321..>2600
                     /locus_tag="VG709_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31586.1"
                     /translation="MQARRALVLVDGEHYPETTRWALEEARHRGIDPVAALLVGGTEK
                     LGADRRLDLGAVPVERAEGEVLGSVRAAVARHAPDVVLDLSDEPVLGYG"
BASE COUNT          412 a          932 c          904 g          352 t
ORIGIN      
        1 gtcgcgtgct cgccgcaggc ctgggcggcc ggcgcgccgt tcctcctcct ccaggcgatg
       61 ctcgggatct cggccgtcgc gcacgagaac ctgctcacgg tcaacaagcc gcatctgccg
      121 ccgtggctca acaccgtcca gctcgacggc atgcggggcg gcgagagccg catctcgctc
      181 gagttccgcc gcgacggcga cacgacgtcg ttctcgatga cgtcgaagga aggcgacgtc
      241 cgcgtcgtga tggaggagta ggcgctcagc ggatacaggg gccggtgtcg acctcgcggg
      301 tcgagccgag cgcctggcgg acgcgcggga gcacctcctc ggacgtgagc gcgtagccca
      361 cgccgtcgtt cgtcgtcgac gcggcgaaca cgacgccggc cacctggccg tccggggtca
      421 cgaacgggcc cccggagttc cccggctgca ccggcgcctg gagctcgtag acctcacgcc
      481 ggacggtgtc gtcgttgtag atgtcgtagc cgagcggcga cagcgtctgt ctgaccgcgg
      541 ccggatcgcc ctgcaggttg ccgccgcgcg ggtacccgag caccgcgccg accgcgcccc
      601 ggtcgaggac gccctggagc aggtcgaggt caggcccggg cgccgacccg acgcgcagca
      661 cggcgaggtc gaggtcgtcg tcgaacagga ccgtggtcgc gtcgaacgtg gcgccgccct
      721 gctgctgcac ctgcggcgcg tccactcccg cgacgacgtg cgcgttcgtc acgacgtagt
      781 tcggctcgac gacgaagccg ctcccctcct ggatgcgccc gcaggcctcg ccgacgatct
      841 tcacggtgct ctcgtcggcc gcccggaacg cgcgtcccac ctcgccgcgg ctcggcccct
      901 ggaccggccc tgccggcgcc ggaggcagat ccgcgaagac gtccgggaac ccgaaccggt
      961 tcaggaactg ccggacctgt gagagaacgg caggaggcct cgggagaacc gtgtcgatcc
     1021 cgcgcacgac cgccgagccg cggatctggc gcgccagcgt ggggaacggt ccctgtacga
     1081 ggttgaacgc gaggaaccag accgcgagga gcacggcgac gccggcgacg acggagcccg
     1141 cggcctggtc gacggggtcg agcacgctcc gccgcgcggc gatccagacg cgtttcccga
     1201 tcatccagcc gatcgcgtcg ccgatcgctg cgaggagcag gagcgtcgtc agcgctatca
     1261 cggcctggcc gaacggctcg acgatgaacc ccgcgacgac cggcgcgagc agcgcgccgg
     1321 cgagcagccc gatgaagagg ccggtgtacg tgacgagctg gagggccgcg ccccgtcgga
     1381 accccgtcac gaccgagaag acgacgaccg cggtcacgac gaggtcgagg acgttcatcg
     1441 cggcatccta tctaccgtcc tctcgcccgc cgggcgctac actgcttgcg agcgatgacg
     1501 atcaccatcg cgcacatctc cgatccccac gtcggctcgc cgtatttcgt gccgaacctg
     1561 atgaaccgcg tcatcgtcga gctgaacgag ctcgagcccg acgcggtgat ctgcaccggc
     1621 gacttcacga acttcgggta ccggcaggag ttcaagacgg cggtcgcgta cctcgaccgc
     1681 atccgggcac ccgtctactc ggtgccgggc aaccacgacg cgcgcaacgt gggctacgtc
     1741 cacttcgagg agctgatcgg gccgcgacac tgggccgtgg acatccgcgg cgtccgcatc
     1801 gtcggcgcgg actcgagcga gccggacctg gaccacgggc agatcggtcg cgagcggtac
     1861 gagtggctcc gcgagcagtt ctcggtgccg gccgacctca aggtcttcgc gatccaccac
     1921 cacctgctgc cggtgcccgg aaccggccgc gaacggaaca tcgtgtacga cgccggcgac
     1981 ctgctcgagg tcctgatcga cgccggaacg cacgtcgtcc tgaccggtca caagcacgtg
     2041 ccgtacgtgt ggcgtctcga gaacctctac gtcgcgaacg cggggacgtg cgcgtcgctc
     2101 cggctgcgcg gccacacgaa gccctgctac aacgtgatcg aggtcgaggg tgacgaggtc
     2161 aagatcttcc gccgcttccc cttcggaggg agcacgctca acgcgcactt ctcgatcacg
     2221 agcgggacgc agtaccaccg cgagctcgag aacgtgatct cggacggcag caccgtgctt
     2281 ccgctcgact aggtcgaccg caccgatcga aggagaccac gtgcaggcga gacgcgctct
     2341 cgtcctcgtc gacggcgagc actatcccga gacgacgcgc tgggcgctcg aagaggcgcg
     2401 ccaccggggg atcgatccgg tcgcggcgct gctcgtcggc ggcaccgaga agctcggcgc
     2461 cgatcgcagg ctcgacctcg gggccgtccc ggtggagcga gccgagggcg aagtcctcgg
     2521 ctcggtgcgt gcggcggtcg cccggcacgc accggacgtc gtgctcgacc tgtccgacga
     2581 gccggtgctc ggctacgggg
//
LOCUS       DASZOB010000030         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10425, whole genome shotgun sequence.
ACCESSION   DASZOB010000030 DASZOB010000000
VERSION     DASZOB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10425"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VG709_00490"
     CDS             <1..363
                     /locus_tag="VG709_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWC31587.1"
                     /translation="VANRDDDGYLFIVDRKKDLIIVSGFNVYPAEVEQALALHPKIAE
                     AAVVGVRDERTGEAVKALVVPKPGDELTEEEVMEHCRARLARFKWPRTVEIVGSLPRH
                     VTGKVLRRALRESSEDER"
     gene            360..1049
                     /locus_tag="VG709_00495"
     CDS             360..1049
                     /locus_tag="VG709_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31588.1"
                     /translation="MIALPILAAVISGVFGAHLLVRSATRRTSFEGVWAVALFMFSAA
                     SAALALGVAGGWSSAEFRVYWLFGAVLNVPYLALGESYLLLRNRWVAHALLAVVLAAT
                     AWASAEVRSAPLDASVLANREFFSGSQVLGEEASARTLALVYSYTGTAVLVGGILLSA
                     IRMGGRPELRNRFYGVLLIALGAVVVAAGAAFARAGNLAGFSVTLVAGVSVMYWGFLV
                     ASRPGARASVA"
     gene            complement(963..>1599)
                     /locus_tag="VG709_00500"
     CDS             complement(963..>1599)
                     /locus_tag="VG709_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HWC31589.1"
                     /translation="YLAHGLTTMTTVALLGTLSSLALTAVLARVFVALTELTGFGTEE
                     ATVVSVGAAQVDLAGILLGGVVIGALGAIDDMTVTQASAVWELRSANPRMDRTDLTRA
                     GLRIGRDHVASTVNTLALAYAGASMPVLLLLVLSRQSIGTVLSGEVIATEIVRTLVGS
                     IGLVTAVPITTWLAAWTAGGPDESGDARAGAGPRRDEESPVHHRDPRDERH"
BASE COUNT          195 a          567 c          590 g          247 t
ORIGIN      
        1 gtcgcgaacc gcgacgacga cggctacctg ttcatcgtcg accgcaagaa ggacctcatc
       61 atcgtctcgg ggttcaacgt ctacccggcc gaggtcgagc aggccctagc cctccatccc
      121 aagatcgccg aggccgccgt cgtcggagtc cgcgacgagc gcaccgggga ggcggtgaag
      181 gcgctggtcg tccccaagcc cggcgacgag ctcaccgagg aggaggtcat ggagcactgc
      241 cgggcgcggc tcgcgcggtt caagtggccg cggacggtcg agatcgtcgg gtcgctgccg
      301 cgccacgtca ccgggaaggt gctccggcgg gcgctgcgcg aatcctcgga ggacgagcgg
      361 tgatcgcgct cccgatcctc gccgcggtga tctccggcgt gttcggcgcc catctgctcg
      421 tgcgctccgc gacgcgccgg acgtcgttcg agggggtctg ggccgtggcg ctgttcatgt
      481 tctcggctgc gtcggccgcg ctcgcgctcg gcgtcgcggg tggttggtcg agcgcggagt
      541 tccgcgtgta ctggctgttc ggcgccgtcc tcaacgtccc ctatctcgcg ctgggcgagt
      601 cgtacctctt gctgcgcaac cggtgggtgg cgcacgcgct cctcgccgtc gtgctcgccg
      661 cgacggcctg ggcctcggcg gaggtccggt cggccccgct cgacgcgtcc gtgctcgcga
      721 accgggagtt cttctccggc agccaggtgc tcggagagga ggcctcggca cggacgctcg
      781 cgctcgtgta ctcgtacacg gggacggcgg tgctcgtcgg cgggatcctg ctctcggcga
      841 tccggatggg cggacgcccg gagcttcgga accgcttcta cggcgtcctt ctcatcgcgc
      901 tcggcgcggt ggtcgtcgcg gcgggcgcgg ccttcgcgcg cgccggcaac ctcgcggggt
      961 tctcagtgac gctcgtcgcg ggggtctcgg tgatgtactg gggattcctc gtcgcgtcgc
     1021 ggcccggcgc ccgcgcgagc gtcgcctgat tcatccggcc cgccggccgt ccatgcggcg
     1081 agccacgtgg tgatcgggac ggccgtcacg aggccgatgc tcccgacgag cgtccggacg
     1141 atctcggtgg cgatgacctc gccggagagc acggtcccga tcgactggcg cgacagcacg
     1201 aggagcagca gcacgggcat cgaggccccc gcgtacgcga gcgcgagcgt gttcacggtc
     1261 gaggcgacgt ggtcgcgccc gatccgcagg cccgcgcgcg tgaggtcggt gcggtccatc
     1321 cgcgggttcg cgctgcgcag ctcccagacc gccgacgcct gcgtgaccgt catgtcgtcg
     1381 atcgcgccga gcgcgccgat caccacgccg ccgaggagga tccccgcgag atcgacctgc
     1441 gcggcgccga cgctcacgac cgtggcctcc tcggttccga accccgtgag ctcggtgagc
     1501 gcgacgaaca cgcgcgcgag caccgcggtg agcgccagcg acgagagcgt cccgagcagc
     1561 gccacggtcg tcatcgtcgt gaggccgtgc gcgaggtac
//
LOCUS       DASZOB010000031         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131595, whole genome shotgun sequence.
ACCESSION   DASZOB010000031 DASZOB010000000
VERSION     DASZOB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131595"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..297)
                     /locus_tag="VG709_00505"
     tRNA            complement(223..297)
                     /locus_tag="VG709_00505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Pro,seq:ggg)
     gene            complement(342..1682)
                     /gene="der"
                     /locus_tag="VG709_00510"
     CDS             complement(342..1682)
                     /gene="der"
                     /locus_tag="VG709_00510"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWC31590.1"
                     /translation="MAADAPAGRPVVAVVGRPNVGKSSLVNRVLGRREAIVEATPGVT
                     RDRHSFVGEWAGRSFEIVDTGGLEPGAAGLEQRVAEQAQVAIETADVIVLVVDASVGP
                     LQDDLVVADRLRAAGKPVLVVANKVDDPSDLSSAAAFFRLGLGEPIALSAVHGTGSGD
                     FLDALAAALPPDRDEGDGSWASLAIVGRPNVGKSSLLNGLTRSARAIVDARPGTTRDP
                     VDSYVAVDDERTLRIVDTAGMRRQVRIEDPLEYFSFLRSRRTLARVDAALLVVDASEG
                     VTAHDQRIAEEIIDHGRACVVTLNKWDLVPAEPSDRARLDHAIDERLRFLPWAPRIRT
                     SALSGRGIDKLLPAVDAAVAAHRHRLATSEVNRIVRDAQAARPHPRTRGKAVRIFYAV
                     QAEVAPPTFVLFANSTLETGYLRYVERSIRAHEPFTGTPLHVTPRLRTRPEVDR"
     gene            complement(1672..1824)
                     /locus_tag="VG709_00515"
     CDS             complement(1672..1824)
                     /locus_tag="VG709_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWC31591.1"
                     /translation="MAARDEADSRRAASPLQKAPDAVEIDTTGRSIAEVTDLIVSLVK
                     QRLGGR"
BASE COUNT          280 a          643 c          661 g          256 t
ORIGIN      
        1 gcgtagagga cgaccgcggc gttcgtctcg ccggcgacga acagccacac gaagaccggc
       61 acggtagcga gtcgcaggaa cgagagcaca ttcggaagcg agaggacccg acggtttgca
      121 gcggccgccg ccggcggggc cgacgtctgc ggtgtagcca gggtgccccc tctctggtcg
      181 gcccgaggtc cgggtcggga tcgtcgctga gtcgcggcgc cgtcgggacg gccggatttg
      241 aaccggcgac ccctggtccc ccagaccagt gcgctaacca agctgcgcca cgtcccgttg
      301 gcccgcatca tatccgtccg cctgctccgc tccggaggcg ctcaacggtc gacctcaggt
      361 ctagtcctga gccgcggcgt gacgtgaagc ggcgtcccgg tgaacggctc gtgcgcgcgg
      421 atgctgcgct cgacgtagcg caggtagccg gtctcgaggg tgctgttggc gaacagtaca
      481 aaggtcggag gagcgacctc cgcctgcacc gcgtagaaga tgcgcacggc cttaccgcgc
      541 gtccgcgggt gaggacgcgc ggcttgcgcg tcgcggacga tgcgattcac ctccgaggtc
      601 gccagccggt ggcggtgcgc cgcgacggcc gcgtccacag cgggcaagag cttgtcgatc
      661 ccgcggccgc tcagggcaga ggtccttatg cgcggggccc acggcaggaa gcgcaaccgc
      721 tcgtcgatcg cgtgatccag ccgcgcgcgg tcgctcggct cggccggaac gaggtcccac
      781 ttgttgagag tgacgacgca ggcccggccg tggtcgatga tctcctcggc gatgcgctgg
      841 tcgtgcgcgg tgacgccctc gctcgcgtcg acgactagca gcgcggcatc gacgcgcgcg
      901 agcgtccggc gggagcgcaa gaagctgaag tattcgagcg ggtcctcgat ccggacctgt
      961 ctcctcatgc cggccgtgtc gacgatgcgc agcgtgcgtt cgtcgtcgac ggcgacatag
     1021 gagtcgaccg gatcgcgggt cgtccccggg cgcgcgtcga cgatcgctcg ggcactccgg
     1081 gtcagcccgt tgaggagcga ggacttgccg acgttggggc ggccgacgat cgcgagcgat
     1141 gcccaggagc cgtcgccctc gtcgcggtct ggcggcaagg ccgcggccag ggcgtcgagg
     1201 aaatcgcccg atcccgttcc gtggaccgcg gacagcgcga tcggctcgcc cagaccgagc
     1261 cggaagaagg ccgccgccga ggagagatcg gacggatcgt cgaccttgtt cgccacaacc
     1321 agcacgggct tgccggcggc gcgcaggcga tcggcaacaa cgaggtcgtc ctgaagaggc
     1381 cccaccgacg cgtcgacgac caagacgatg acgtccgccg tctcgatcgc gacctgagcc
     1441 tgttccgcaa cgcgctgctc gagccccgcg gcccccggct cgagcccgcc cgtgtcgact
     1501 atctcgaacg acctgcccgc ccactcgccg acgaaggaat gccgatcgcg cgtgactccc
     1561 ggcgtggcct cgacgatcgc ctcgcggcga ccgaggacgc ggttgaccag cgacgacttg
     1621 cccacgttgg gacgcccgac gaccgcgacg acgggacggc cggccggcgc gtcagcggcc
     1681 accgaggcgc tgcttcacga gcgagacgat caggtcggtc acctcggcga tcgacctgcc
     1741 ggtcgtgtcg atctcgaccg catcgggggc cttctgcagc ggtgacgcgg cgcggcgcga
     1801 gtcggcctcg tcacgcgccg cgatcgtcgc ctgagtaccc
//
LOCUS       DASZOB010000032         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_182469, whole genome shotgun sequence.
ACCESSION   DASZOB010000032 DASZOB010000000
VERSION     DASZOB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182469"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1615
                     /locus_tag="VG709_00520"
     CDS             266..1615
                     /locus_tag="VG709_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31592.1"
                     /translation="MNAAPAIFRNYVYVGNRTDGSVDDPHPGVLVVDVRDPSDPEVVA
                     EIGPPNEGNVSETSRELRVWPQQRLLLVMNFQCSAILHECVSNADVTGSLEWSIKFYD
                     LSGENAASPKLVATYVPSRVPHEMHLWVDPAKPNTRALLYLSTPTSRTDRPNLIVTDI
                     SKARKDVFSEIVHWRGNDEFTAEEREGRDVRLHSMGTSADGRRTYLAYLGGGFLVLDT
                     SEVAAKVRDPEVRLLTPPSNSPRWPNQTVHSAFKFPGRDLAITTDEVYGDLLDPLVRP
                     ENEFGCPWGWVHIIDISDESRPTIVGRYLIDENRQEYCESADGQDPSNTFFTSYSAHN
                     PTVLPNLAFVTWHSGGLQAFAVGPDGRTKPAGFFSPEPLPFVFTEDPALSLGRNKVVM
                     WSFPIIKDGLIYAVDVRNGLYILRYTGPRAAEVAEIRFLEGNSNLGDALRLEGISSG"
     gene            1705..>2500
                     /locus_tag="VG709_00525"
     CDS             1705..>2500
                     /locus_tag="VG709_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HWC31593.1"
                     /translation="MPDTELLDVGGREVRVTNPGKVFFPKTGATKMDLVRYYLAVGEG
                     AVRGALERPVILHRYPNGVDGEDFYQKRIAGNVPDWLRTVTIRFPSGRSARELVMADM
                     AHLVWHANLGCIDINPWPVRATDVDHPDELRVDLDPTPRIPFASVRKVALVVREVLDE
                     HGLIGFPKTSGKRGIHVSVRIEPRWGFTEVRRAALALAREVERRSPLATTAWWKEERR
                     GVFVDYNQNARDRTVASAYSVRPVADARVSCPLDWDEVPDVEPRELR"
BASE COUNT          410 a          858 c          855 g          377 t
ORIGIN      
        1 gtcgtggacc gcgtccgcga cggagtccgc gagggcgtcg aggacgccga gaaggagcgc
       61 tgagctcccg cgtcgaagcg gtccatacgg gtacgtaacg gaccgccagc gtcggaggag
      121 gtcacgatga ggaccgtgcg aatcgtctgc gtggccctgt cgctctcggt gctttcggtt
      181 cccgcgcccg tagggggcgg cgaccgagcc gggcacggcc gcgaacttcg agctcgtagg
      241 ccacgagccg ctgttcaacc gggggatgaa cgccgccccg gcgatcttcc gtaactacgt
      301 ctacgtcggg aaccgcaccg acggatccgt ggacgacccg cacccggggg tgctcgtcgt
      361 cgacgtgcga gatccgtcgg atcccgaggt cgtggccgag atcggacctc cgaacgaggg
      421 gaacgtcagc gagacgtctc gcgaactccg cgtgtggccg cagcagcgac tcctcctcgt
      481 catgaacttc cagtgcagcg cgatcctgca cgagtgcgtc tcgaacgcag acgtgacggg
      541 atcgctcgag tggagcatca agttctacga cctgtcggga gagaacgcgg cctcaccgaa
      601 gctcgtcgcg acgtacgtgc cgtctcgtgt cccgcacgag atgcacctgt gggtggaccc
      661 ggcgaagccg aacacacgag cgctgctcta cctgtcgacg ccgacgagcc gcaccgaccg
      721 tcccaacctc atcgtcaccg acatcagcaa ggcacggaaa gacgtcttca gcgagatcgt
      781 ccactggagg ggcaacgacg agttcacggc cgaggaacgc gagggccgcg acgtgcgcct
      841 ccactcgatg ggcacgtcgg ccgacggacg gcgaacgtac ctcgcgtacc tcggcggcgg
      901 gttcctggtg ctcgacacca gcgaggtcgc ggcgaaggtc cgggacccag aggtcaggct
      961 cctgactccg ccgagcaact cgccgcggtg gccgaaccag acggttcact ccgcgttcaa
     1021 gttcccgggg cgcgacctcg cgatcacgac cgacgaggtc tacggggacc tcctcgatcc
     1081 gctcgtccgg cccgagaacg agttcggctg tccgtggggc tgggtccaca tcatcgacat
     1141 cagcgacgag tcgaggccga cgatcgtggg ccgctacctg atcgacgaga accgccagga
     1201 gtactgcgaa tcggccgacg ggcaggaccc gtccaacacc ttcttcacga gctactcggc
     1261 gcacaacccg accgtcctac cgaacctcgc gttcgtgacc tggcactccg gtgggctcca
     1321 ggcgttcgcc gtcggccccg acgggcggac gaagccggcc gggttcttct cgcccgagcc
     1381 gctcccgttc gtgttcaccg aggacccggc gctctcgctc ggccggaaca aggtcgtgat
     1441 gtggagcttc ccgatcatca aggacgggct gatctacgcc gtggacgtcc ggaacggcct
     1501 ctacatcctg cggtacacgg gtccccgggc ggcggaggta gccgagatcc ggttcctcga
     1561 gggcaactcc aacctcggcg atgccttgcg gcttgagggc atctcttcgg gctgacgcgc
     1621 ccgttcggag gaatcggcgc caacgaggga cgattccaac cggttttcaa caggtgtgga
     1681 ggccgggctg cgagaatcgc ccccatgccg gacacggagt tgctcgacgt cggcggccgc
     1741 gaggtccgcg tcacgaatcc cggcaaggtc ttcttcccga agaccggcgc cacgaagatg
     1801 gacctcgtcc gctactacct cgccgtgggc gagggggccg tccgcggagc gctcgagcgc
     1861 cccgtgatcc tccaccgcta cccgaacggc gtggacgggg aggacttcta ccagaagcgg
     1921 atcgccggga acgtgccgga ctggctccgg accgtcacga tccggttccc gagcggccgt
     1981 tcggcacgcg agctggtgat ggcggacatg gcgcatctcg tctggcacgc gaacctcggc
     2041 tgcatcgaca tcaacccgtg gccggtccgc gccacggacg tcgaccatcc cgacgagttg
     2101 cgcgtcgacc tcgacccgac gccgcggatc ccgttcgcga gcgtgcgtaa ggtcgcgctg
     2161 gtcgtgcgcg aggtgctcga cgagcacggc ctcatcgggt tcccgaagac cagcgggaag
     2221 cgcgggatcc acgtctcggt tcggatcgag ccgcggtggg ggttcaccga ggtccgccgc
     2281 gcggcgctcg cgctcgctcg cgaagtcgag cgccgctcgc cgctcgcgac gaccgcgtgg
     2341 tggaaggagg agcggcgcgg cgtgttcgtc gactacaacc agaacgcgcg ggaccggacg
     2401 gtggcttcgg cgtactccgt ccgtcccgtc gccgacgctc gcgtgtcgtg cccgctcgac
     2461 tgggatgagg tccccgacgt cgagccgcgc gagctgcggc
//
LOCUS       DASZOB010000033         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_141988, whole genome shotgun sequence.
ACCESSION   DASZOB010000033 DASZOB010000000
VERSION     DASZOB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141988"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VG709_00530"
     CDS             <1..234
                     /locus_tag="VG709_00530"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HWC31594.1"
                     /translation="VFHTVTLEYFDDESRARVEAAVEEAGADATEDAPLAWLGMEIEQ
                     MAFPVRLAMWPWQPEERIVGHARAHGPPVRWLA"
     gene            complement(283..615)
                     /locus_tag="VG709_00535"
     CDS             complement(283..615)
                     /locus_tag="VG709_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31595.1"
                     /translation="MTDWRWEEACEQVRARERNERSPGSRPGEDPTRLGLYVCECGDT
                     SCRVPIMLTREEYDLVRSDATWFAVAPNHENPEIDHVVSEHPRFSTVAKVTGDLVRVA
                     RSETARAS"
     gene            complement(608..940)
                     /locus_tag="VG709_00540"
     CDS             complement(608..940)
                     /locus_tag="VG709_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWC31596.1"
                     /translation="MDWTGSLIIETQSVDGTVTLAVSGELDMATRDRLLGALEWLEAN
                     GEEAITVDLTGLSFIDATGLHALIRARDSARMRGATLEFRNPSPAVRRLISLVGPEGL
                     VEEVGDGD"
     gene            1140..1748
                     /locus_tag="VG709_00545"
     CDS             1140..1748
                     /locus_tag="VG709_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWC31597.1"
                     /translation="MTPTNRRLRVLVAEGRKELLASVSEAIERLGHEVVEETDLGSVA
                     QVTASKDPEVAIVILGESSQHALGMIDRIVHEATCPVIAVIDVQDRSFVNEAAQRGIF
                     AYVSGGDDPEVLQSSIDIVLRRFREYHDLEGAFGRRAVTERAKGVLMERHGVDEKEAF
                     DMLRTRARASNRKIVDVAQSVLETHVLLPRGSTPREDDELAR"
BASE COUNT          291 a          648 c          658 g          280 t
ORIGIN      
        1 gtcttccaca ccgtgacgct cgagtacttc gacgacgagt cgcgcgcacg ggtggaagcg
       61 gcggtcgagg aagccggcgc cgacgcgacc gaggacgcgc cgctcgcgtg gctcgggatg
      121 gagatagagc agatggcgtt cccggtgcgc ctcgcgatgt ggccgtggca gcccgaggag
      181 cggatcgtcg gccacgcacg cgcgcatggg ccgccggtcc ggtggctcgc atgagttccg
      241 cataagcctc cggggccggt cagggaagcc cccggctcgg gatcagctcg cgcgggcggt
      301 ctcgctgcgc gcgacgcgga cgaggtcccc cgtcaccttc gcgaccgtcg agaaccgcgg
      361 gtgctccgag accacgtgat cgatctcggg gttctcgtgg ttcggggcga ccgcgaacca
      421 cgtcgcgtcg ctcctcacga ggtcgtactc ctcccgggtc agcatgatgg ggacacggca
      481 ggaggtatct ccgcactcgc acacgtagag gccgaggcga gtggggtctt ctcccggtcg
      541 gctgcccggc gagcgctcgt tccgttcgcg tgcgcgcacc tgctcacacg cctcctccca
      601 gcgccagtca gtcaccatcg ccgacctcct cgaccagccc ttcgggaccg acgagcgaga
      661 tcagccggcg cacggccggc gacgggttgc ggaactcgag cgtcgcgccg cgcatgcggg
      721 ccgagtcgcg cgctcgtatc agcgcgtgca ggccggtcgc gtcgatgaac gagagacccg
      781 tgaggtcgac cgtgatggcc tcctcgccgt tcgcctccaa ccattcgagg gcgccgagga
      841 gccgatcacg ggtagccatg tcgagctcgc cggagacggc gagcgtcacg gtgccgtcga
      901 ccgactgcgt ttcgatgatg agggagccgg tccagtccac ggtcctgccg cctttcacac
      961 acgtcgaccg gaggtcgggg tcgactcgtg atccggcccg gacgttgggg accgaggacc
     1021 cggccgtgag ctggagccga gcacgtccca ccctacgccg attacggcga accggcaacc
     1081 gagcgctcca ccttcgttgc gaatccaggt cgggtaccgt agcgacgagg gacgaatccg
     1141 tgacacctac caaccgccgt ctgcgagtgc tcgtcgccga gggccgcaag gagcttctcg
     1201 cgagcgtgtc cgaagcgatc gagcgcttgg ggcacgaggt cgtcgaggag accgatctcg
     1261 gctcggtggc gcaggtgacc gcatccaagg acccggaggt cgctatcgtc atcctcgggg
     1321 agagcagcca gcacgccctc gggatgatcg acaggatcgt gcacgaggcg acgtgcccgg
     1381 tgatcgccgt catcgacgtg caggaccgca gcttcgtgaa cgaggcggcc cagcggggga
     1441 tcttcgcgta cgtgtccggc ggcgacgatc cggaagtgct ccagagctcc atcgacatcg
     1501 tcctccgcag gttccgcgag tatcacgatc tcgagggagc gttcgggcgc cgggccgtca
     1561 ccgagcgcgc gaagggagtc ctgatggagc ggcatggcgt cgacgagaag gaggcgttcg
     1621 acatgctgcg cacgcgcgcg cgagcctcga accgcaagat cgtggacgtc gcgcagtccg
     1681 tcctcgagac gcacgtcctg ctaccgcgcg ggtcgacgcc ccgcgaggac gacgagctcg
     1741 ctcgctgaac cggggcgtcg ttcgtctacc gtctctgcga tgccgccgcc gctcgagatc
     1801 cccctccgcg ggccacgagg cgagcccgtc gatctccgcc gtacgctttc ctcgcacggg
     1861 atcgcgcacc tgccgcc
//
LOCUS       DASZOB010000034         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_182521, whole genome shotgun sequence.
ACCESSION   DASZOB010000034 DASZOB010000000
VERSION     DASZOB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182521"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..339)
                     /locus_tag="VG709_00550"
     CDS             complement(31..339)
                     /locus_tag="VG709_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31598.1"
                     /translation="MDTYTDSVERVADRLIDVVRKADDAAVAAAAGASNAVARFMPDL
                     RGSALERLPKPEQFVRVYFDFVERLVKTQRTYSMDLVKAIEPVSRKIWPAPKVRKAAA
                     "
     gene            complement(409..768)
                     /locus_tag="VG709_00555"
     CDS             complement(409..768)
                     /locus_tag="VG709_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWC31599.1"
                     /translation="MAAKRTKRREASPLGEFIQHQRRLARLSLRQLADLAKVSNAYLS
                     QVERGLYRPSAKVLKHVAGALDVSAETLYAKAGFLEDDPDADVPRTEDAIRLDPDLTE
                     EQKDTLLRVYRSLAGRA"
     gene            complement(833..>1865)
                     /locus_tag="VG709_00560"
     CDS             complement(833..>1865)
                     /locus_tag="VG709_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWC31600.1"
                     /translation="YVDDWEEIGAPEPFWFGRIFPEMRECKRLFAELVGAAPEELAIT
                     TNVSFALSSILSCLDLSGDRNRIVLTELDLPTDGHVALAQRRRGAEVSFLPSLDGLTV
                     PVEAFADAIDERTALVIINRVLYRSSALLDVGEICRLAREAGAWTVIDDFHGAGIVPI
                     DVHEVGCDFYTTGVLKWLCGGPGLTFLYARRDLLPSLEPTVAGWWGQREPFAFNGREL
                     DWHPTARRLETGTHAAPIAFIARGGLEIIAEVSPDRIRDRQGELTDHVMERADAMGLA
                     VRTPRDRKARGGVVNVRVGDRAAHIAAALFERDVCVDHRADGIRISPHFFNTEGDVDR
                     LFDALSSLL"
BASE COUNT          298 a          646 c          637 g          284 t
ORIGIN      
        1 gcggggggct tcgacgctcg tcgccgtggc ctatgcggcg gccttgcgga ccttcggcgc
       61 gggccagatc ttgcgggaaa ccggctcgat ggccttgacg agatccatcg agtacgtgcg
      121 ctgggtcttc accagacgct cgacgaagtc gaagtacacg cggacgaact gctccggctt
      181 cgggagacgc tccagggcgg acccgcgaag atctggcatg aaccgcgcga cggcgttgct
      241 cgcgcccgcc gcggccgcga cggccgcgtc gtctgccttg cgaacgacgt cgatcagacg
      301 gtctgcgacg cgctcgacgc tgtctgtgta cgtgtccatg gcttcctcct tggtgatggg
      361 gccgctgtgg cctccggtgc taagattacc aagcactcgc tcggcatgtc aagcacggcc
      421 ggcgaggctc cggtagacgc gaaggagggt gtccttctgc tcctcggtga ggtcggggtc
      481 gagccggatc gcgtcctccg tcctcggaac gtcggcatcc ggatcgtcct cgaggaatcc
      541 ggccttggcg tagagcgtct ccgcggagac gtccagcgcg cccgcgacgt gcttgaggac
      601 cttcgcggac ggccggtaca gcccgcgctc gacctggctc aggtacgcgt tcgagacctt
      661 cgcgaggtcg gcgagctgac gcaacgacag ccgggcgagc cgccgctggt gctggatgaa
      721 ctccccaaga ggggacgcct cgcggcgttt cgtccgcttc gccgccatat ggccagccta
      781 ccatattgct tggtatacca agcggcggcg gccgccggca cagcgccgcc ggtcagagca
      841 gcgaggaaag cgcgtcgaag agccggtcga catcgccctc ggtgttgaag aagtgcggcg
      901 agatcctgat gccgtccgcg cgatggtcga cgcagacgtc gcgctcgaac agcgccgcgg
      961 cgatgtgggc cgcgcggtca ccgacgcgca cattgaccac gcctccgcga gccttccggt
     1021 cgcgcggcgt ccggacggcg agacccatgg cgtccgcgcg ctccatcacg tgatcggtga
     1081 gctcaccctg gcggtcgcgt atccggtccg gcgacacctc ggcgatgatc tcgaggcctc
     1141 cgcgcgcgat gaaggcgatc ggcgccgcat gcgtaccggt ctcgagccgg cgcgccgtcg
     1201 gatgccagtc gagctcacgc ccgttgaacg cgaagggctc ccgctggccc caccaacccg
     1261 cgacggtcgg ctcgagcgac ggcaggaggt cgcggcgcgc gtacaggaac gtgagccctg
     1321 ggccgccgca cagccacttg agcaccccgg tcgtgtagaa gtcgcacccg acctcgtgca
     1381 cgtcgatcgg cacgatgccc gcaccgtgga agtcgtcgat aacggtccac gcgcccgcct
     1441 cgcgcgcgag gcggcagatc tctccgacgt cgagcagcgc actcgagcgg tacagcacgc
     1501 ggttgatgat cacgagcgcg gtgcgctcgt cgatcgcgtc ggcgaacgcc tcgaccggaa
     1561 ccgtgagccc gtccaggctc ggcaggaacg atacctccgc accgcgccga cgttgggcca
     1621 gcgcgacgtg cccgtccgtc ggtaaatcga gctcggtgag cactatccga ttgcgatcgc
     1681 ccgagagatc gagacacgac aggatcgacg aaagcgcgaa cgagacgttg gtcgtgatcg
     1741 cgagctcctc cggtgccgcg ccgacgagct cggcgaacag ccgcttgcac tcccgcatct
     1801 ccgggaagat ccgcccgaac cagaacggct cgggcgcgcc gatctcctcc cagtcgtcga
     1861 cgtac
//
LOCUS       DASZOB010000035         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_20871, whole genome shotgun sequence.
ACCESSION   DASZOB010000035 DASZOB010000000
VERSION     DASZOB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20871"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VG709_00565"
     CDS             complement(<1..1062)
                     /locus_tag="VG709_00565"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWC31601.1"
                     /translation="MRIARRIEALPPYLFAELDRRLAEKRAQGVDVISLGVGDPDTPT
                     PEHVVQAGQRAAADPSTHRYPSYFGMPEFRRVIADWQRRRFGVELDPDTEVLPLIGSK
                     EGLAHLSVAYVDPGDEALVPDPGYPVYAIGTRLAGGTPVAMRLDVAGGFVPDLDALRP
                     GPSTKVLWLNYPSNPTAAVADLPFFERAVAFAREHGLLLAHDAAYSEMTYDGYVAPSV
                     LEVPGAKDVAVEFNSVSKSQNMTGWRVGWMAGNADAVRARGTVKTNIDSGIWNAVQRA
                     AIAALEGPEDHLDGLRALYQKRRDLVVSTFRDLGWEVEPPLGSIYVWAPTPGGEASAT
                     FATRLLDATGVVVAPGTGYG"
     gene            complement(1059..>1623)
                     /gene="dapF"
                     /locus_tag="VG709_00570"
     CDS             complement(1059..>1623)
                     /gene="dapF"
                     /locus_tag="VG709_00570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWC31602.1"
                     /translation="SMEVDGRRVRSVTVDMGPPAFERGRLPMTGDPTSTFVNDAWQVG
                     DRRLRATALSMGNPHLVLFVEPDHDLAALDVRALGSAIEHDPAFPERVNVEFVKPLEG
                     RLAMRVWERGSGETMACGTGACAVLVAANLAGLAKRASQVDVPGGTLSIDWRDDDHVF
                     LTGPATFVFEAELEDPWLDAARGGSLA"
BASE COUNT          225 a          599 c          589 g          210 t
ORIGIN      
        1 gccgtacccg gtccccgggg cgacgacgac gccggtcgcg tcgaggagcc gcgtggcgaa
       61 cgtcgccgac gcctcgccgc cgggcgtggg cgcccacacg tagatggagc cgagcggcgg
      121 ttcgacctcc cacccgaggt cgcggaacgt cgagacgacc aggtcgcgcc gcttctggta
      181 cagcgcgcgg agcccgtcga gatggtcctc cggtccttcg agcgcggcga tcgcggcccg
      241 ctgcacggcg ttccagatcc cggagtcgat gttcgtcttg accgtccccc gcgcgcggac
      301 cgcgtcggcg ttgccggcca tccagccgac gcgccagccg gtcatgttct gcgacttcga
      361 gaccgagttg aactcgaccg cgacgtcctt cgcgcccggc acctcgagca cgctcggcgc
      421 gacgtagccg tcgtaggtca tctccgagta cgcggcgtcg tgcgcgagca gcaggccgtg
      481 ttcgcgcgcg aacgcgacgg cccgctcgaa gaacggcagg tcggcgacgg ccgccgtcgg
      541 gttgctcggg tagttgagcc acaggacctt cgtgctgggg cccggccgga gcgcgtcgag
      601 gtcgggcacg aagccgccgg cgacgtcgag ccgcatcgcc accggcgtcc cgcccgcgag
      661 ccgagtgccg atcgcgtaca ccgggtagcc ggggtcgggc acgagcgctt cgtcgcccgg
      721 gtccacatag gcgacggaca ggtgcgcgag cccctccttc gagccgatca gcgggagcac
      781 ctcggtgtcg gggtcgagct cgacgccgaa ccgccgccgt tgccagtcgg cgatgacgcg
      841 gcggaactcc ggcatcccga agtaggaggg atagcggtgc gtgctggggt ccgcggcggc
      901 gcgctggccc gcctgcacca cgtgctccgg cgtcggcgtg tccgggtcgc cgacaccgag
      961 cgagatgacg tcgacgccct gcgcgcgctt ctccgccagc cggcgatcca gctcggcgaa
     1021 gaggtacggc ggcagcgcct cgatccggcg ggcgatcctc acgcgagcga gccgccgcgc
     1081 gcggcgtcga gccacgggtc ctcgagctcc gcctcgaaga cgaacgtcgc ggggccggtc
     1141 aggaagacgt ggtcgtcgtc gcgccagtcg atcgagagcg tcccgccggg aacgtcgacc
     1201 tgcgaggcgc gcttcgcgag ccccgcgagg ttcgccgcca cgaggacggc gcacgccccg
     1261 gtgccacacg ccatcgtctc ccccgaaccg cgctcccaga cgcgcatcgc gaggcggccc
     1321 tcgagcggct tcacgaactc gacgttgacg cgctcgggga acgccgggtc gtgctcgatg
     1381 gccgaaccca gagcccggac gtcgagcgcc gcgaggtcgt ggtcgggctc gacgaacagc
     1441 acgaggtgcg gattccccat cgacagggcc gtcgcgcgca gacgccggtc acccacctgc
     1501 cacgcgtcgt tcacgaacgt cgaggtgggg tctccggtca tgggcaggcg tccccgctcg
     1561 aacgcgggcg gccccatgtc gacggtgacg ctgcggaccc gccgtccgtc gacctccatc
     1621 gac
//
LOCUS       DASZOB010000036         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51232, whole genome shotgun sequence.
ACCESSION   DASZOB010000036 DASZOB010000000
VERSION     DASZOB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51232"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..799
                     /locus_tag="VG709_00575"
     CDS             8..799
                     /locus_tag="VG709_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWC31603.1"
                     /translation="MARELRITDLRMLIEAGSGHPGGTLSAADMVAALYFHKLRLRPE
                     EPAWPGRDRFVLSKGHCIPIVYAALAKRGFFDEAELMTLRKLGSRLQGHPDRTRLPGI
                     EASTGSLGQGLSVAVGMALAGKLDGADYRVYCMLGDGEIQEGQVWEAAMFAPRHGLDN
                     LCAILDANQVQQTAKVADILDEEPLVAKWRAFNWHVREIDGHDMEQIVDALDEAELVS
                     GRPTMIVSHTVKGKGVPWMELDPEWHGKAPDGEKAEEALAALEER"
     gene            799..1725
                     /locus_tag="VG709_00580"
     CDS             799..1725
                     /locus_tag="VG709_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWC31604.1"
                     /translation="MATATRDAFADALVRLGSDERIVVLTGDLRDSTRTEKFLEKHPD
                     RFFDVGIAESNMIGIASGLALSGKIPWACSFSAFVTGRYDTVRVSVAYQEANVRIVGT
                     HAGIGVGEDGYSQMSLEDVACIRALPNIAIVNPCDAVSTERAVEYLVEHEGPAFLRLT
                     RQKVDDVYGDDFRFELGKAVTVREGSDVAILATGATVQEALKAADALGDLSVRVVDVH
                     TIKPLDEEAVRRAATECRAVVTVEDHSVHGGLGGAAAEVLAAGDGNLASLRMIGMTTF
                     GESGGTDELYDKYGLSGARIAETVRAAIANTG"
     gene            1853..2539
                     /gene="ftsE"
                     /locus_tag="VG709_00585"
     CDS             1853..2539
                     /gene="ftsE"
                     /locus_tag="VG709_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWC31605.1"
                     /translation="MIKTDRITKVYKDSVVALEDVSVDVGKGEFVFIVGPSGSGKSTF
                     IRLLLKEEEPSRGTIHVAGKNIGRLATWKVPYLRRNIGTIFQDFKLLPEKTVFENVGF
                     ALEVIGKPRHVIDRRVPEILELVGLGGKLSNYPDELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPTTSVDIMKLLDRINRTGTTVIMATHDFAIVDAMRRRVIELDQGRVVRD
                     QARGVYGYGA"
BASE COUNT          434 a          859 c          878 g          385 t
ORIGIN      
        1 ctagcggata gcgcgtgagc tccggatcac cgacctccgg atgctcatcg aggcggggtc
       61 cggccacccc ggtgggacgc tgtcggccgc cgacatggtc gcggcgctct acttccacaa
      121 gcttcggctg cggcccgagg agccggcctg gccgggccgc gaccgcttcg tgctctcgaa
      181 aggccactgc atccccatcg tctacgcggc gctcgcgaag cgcggcttct tcgacgaggc
      241 cgagctcatg acgctccgca agctcgggtc gcggctccag ggacacccgg atcggacgcg
      301 gctcccgggg atcgaggcgt cgaccggatc gctcggccag ggcctgtcgg tcgccgtcgg
      361 catggcgctg gccggcaagc tcgacggcgc cgactaccgc gtctactgca tgctcggcga
      421 cggcgagatc caggaagggc aggtgtggga ggccgcgatg tttgcgccgc gccacggcct
      481 cgacaacctc tgcgcgatcc tcgacgcgaa ccaggtgcag cagacggcga aggtcgcgga
      541 catcctcgac gaggagccgc tcgtcgcgaa gtggcgcgcg ttcaactggc acgtccgcga
      601 gatcgacggc cacgacatgg agcagatcgt cgacgcgctc gacgaggccg agctcgtgtc
      661 gggtcgcccg acgatgatcg tctcgcacac cgtgaagggg aagggcgtcc catggatgga
      721 gctcgacccc gaatggcacg gcaaggcgcc cgacggcgaa aaggccgaag aggcgctggc
      781 cgcgctggag gagcgctgat ggcgaccgcg acgcgggacg cattcgccga cgcgctcgtc
      841 cgcctgggat ccgacgagcg catcgtcgtg ctgaccgggg atctgcgcga ctcgacccgc
      901 accgagaagt tcctcgagaa gcaccccgac cggttcttcg acgtcgggat cgccgagtcg
      961 aacatgatcg ggatcgcctc cggcctcgcg ctctcgggga agatcccgtg ggcgtgctcg
     1021 ttctcggcgt tcgtcaccgg ccgctacgac accgtgcggg tctcggtcgc gtaccaggag
     1081 gcgaacgtcc gcatcgtcgg gacgcacgcg ggcatcggtg tgggggagga tggctactcc
     1141 cagatgtcgc tggaggacgt cgcgtgcatc cgcgcgctac cgaacatcgc gatcgtgaat
     1201 ccgtgtgacg ccgtctcgac cgagcgtgcc gtcgagtacc tcgtcgagca cgaggggccg
     1261 gcgttcctcc ggctcacgcg ccagaaggtc gacgacgtgt acggcgacga cttccgcttc
     1321 gagctaggca aggccgtgac ggtccgcgag gggagcgacg tagcgatcct tgcgaccggt
     1381 gcgacggtgc aggaggcgct caaggcggcc gacgcgctcg gcgacctctc ggtccgtgtc
     1441 gtcgacgtcc acacgatcaa gccgctcgac gaggaagccg tccgtcgcgc cgcgacggaa
     1501 tgtcgcgcgg tcgtcacggt cgaggaccac agcgtccacg gcgggctcgg cggcgcggcc
     1561 gccgaggtgc tcgcggccgg cgacggcaac ctcgcatcgc tgcggatgat cgggatgacg
     1621 acgttcgggg agtcgggcgg caccgacgag ctctacgaca agtacggcct cagcggggcc
     1681 cgcatcgccg agacggtgag ggcggcgatc gcaaacaccg ggtagacggc gggctgcagc
     1741 aactgcatga cgatctcacg gcgaatctag cgcccgcctc taacatggtt cacatcactc
     1801 gaggtctctg atagccttac gagccggtct gcccgaccaa ccttcaaacg ccatgatcaa
     1861 gaccgatcgc atcaccaagg tctacaagga ctcggtcgtc gccctcgaag acgtctcggt
     1921 cgacgtgggt aagggcgagt tcgtcttcat cgtcggcccg tccgggtcgg ggaagtcgac
     1981 gttcatacgc ctcttgctca aggaggagga gccaagccgc gggacgatcc acgtcgccgg
     2041 gaagaacatc ggccgcctgg cgacgtggaa ggtgccgtac ctgcggcgca acatcggcac
     2101 gatcttccag gacttcaagc tcctccccga gaagacggtc ttcgagaacg tcggcttcgc
     2161 gctcgaggtc atcgggaaac cgcgccacgt catcgatcgc cgcgtcccgg agatcctcga
     2221 gctggtcggg ctcggcggaa agctctcgaa ctacccggac gagctctccg gcggcgagca
     2281 gcagcgcgtg tcgatcgcgc gtgcgttcgt taaccgcccg ctgatccttc tcgcggacga
     2341 gccgaccggg aatctcgacc cgacgacgtc cgtggacatc atgaagctcc tcgaccgcat
     2401 caaccgcacc ggcacgaccg tcatcatggc gacgcacgac ttcgcgatcg tcgacgcgat
     2461 gcgacgccga gtgatcgagc tcgaccaggg ccgcgtcgtc cgcgaccagg cgcgaggcgt
     2521 ctacggctat ggcgcgtaag ttcgggtact tcttac
//
LOCUS       DASZOB010000037         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_81495, whole genome shotgun sequence.
ACCESSION   DASZOB010000037 DASZOB010000000
VERSION     DASZOB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81495"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VG709_00590"
     CDS             <1..957
                     /locus_tag="VG709_00590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWC31606.1"
                     /translation="IYVKIDGLRRPVPDGVTVARVRRVFALRPRPGALLDIEGVPLER
                     RKYPGHLTLNGKRVSGDAVLRSGDVLEVFDGRDRYEPLSFATRRVSGGRPENPQTVLG
                     TTPGQTIITTGAESGKFVSSVFRPTGGARVPNAVSLTFDDGPSPRYTPAVLRVLRRQR
                     VRATFFVVGYLARRYPDIVRRLEAARMQVGNHSLSHPNAPPFATLATREIERQIAAGA
                     GVLEDLGIEPTVFRPPGGSWSNAVVAAAEARGQRTVLWTVDSRDWAGLPAGAIAERVV
                     SEARPGSIVLLHDGGGNRAPTLQALPRIIRGIRAKGLSFSVL"
     gene            complement(954..>2163)
                     /locus_tag="VG709_00595"
     CDS             complement(954..>2163)
                     /locus_tag="VG709_00595"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWC31607.1"
                     /translation="RWVGADASVEPSTLLAVDSARDWEEFRAAFEGWRCPGQNAVYAD
                     THGNIGYQLGGLYPVRRTGDGTVPVPGWTEDYEWDGWVPYDELPRAYNPEEGFLVTAN
                     NKIHGDDYPHVISRDFLPPFRARRIAQLVTEEPHHTADTFRRIQLDTVSLPAREMLPF
                     LVALEPRDDRQKHALAVLAEWDGDVAAGSSAAAIYEVWCERVARAVLRPMMDADLFTH
                     FHGRREWTNAFQYQVLPTLLRYPTARWFGRDGAEGRDGALRDALDAALDELTQRFGDD
                     IAAWRWGALHRVTFAGRLAMIPDLAEMCTAGVAEVGGDEQTVMQGMYEPEVGYDVVVA
                     PSWRQVIDLGDLDASMGTNTTGQSGNPASPHFNDLFDLWSTGGLHPMPFSRPSVEAAA
                     RGTLRLLPPG"
BASE COUNT          287 a          775 c          782 g          319 t
ORIGIN      
        1 atctacgtga agatcgatgg gctgcggcgc cccgtgcccg acggcgtcac ggtggcccgc
       61 gtccggcgcg tcttcgcgct ccgccccagg cccggcgcgc tgctcgacat cgaaggcgtg
      121 cccctcgagc ggcggaagta ccccggccac ctgaccctca atggcaagcg agtgtccggt
      181 gacgccgtgc tgcgctccgg cgacgttctc gaagtcttcg acggccgaga ccggtacgag
      241 ccgctgtcct tcgcgacgcg ccgcgtgtcc ggggggcggc ccgagaaccc gcagacggtg
      301 ctcggcacga cccccggcca gacgatcatc acgaccggcg ccgagtccgg gaaattcgtg
      361 tcgtccgtct tcaggccgac gggaggagcg agggtcccga acgcggtctc cctgacgttc
      421 gacgacggtc catctccccg gtacaccccc gcggtcctta gggtgctgcg acgtcagcgg
      481 gtgcgcgcca cgttcttcgt cgtcggatac ctcgcgcgcc ggtacccgga catcgtcagg
      541 cgcctggagg cggcccggat gcaggtcggg aaccactcgt tgtcgcaccc gaacgcgccg
      601 ccgttcgcga cgcttgcgac gcgcgagatc gagcgtcaga tcgccgcggg tgccggggtg
      661 ctggaggacc tggggatcga gccgacggtg ttccgcccgc cggggggctc gtggagcaac
      721 gccgtcgtcg ccgccgccga ggcgcgcggc cagcgcacgg tgctgtggac tgtcgacagc
      781 cgcgactggg cgggtctccc ggccggagcg atcgcggagc gcgtcgtctc cgaggcgaga
      841 ccgggctcga tcgtgctcct gcacgacggc ggcgggaacc gcgctccgac gctgcaggcg
      901 ctgccgcgga tcatccgcgg catccgcgcg aagggcctct cgttctccgt gctctagccc
      961 ggcgggagca accgcagcgt gccgcgggcc gcggcctcga cgctcggacg cgagaacggc
     1021 atcgggtgga gtcctccggt gctccagagg tcgaagaggt cgttgaagtg agggctcgcg
     1081 gggttcccgg actgcccggt ggtgttcgtc cccatcgagg cgtcgaggtc gccgaggtcg
     1141 atgacctggc gccaggacgg cgccacgacg acgtcgtagc cgacctccgg ctcgtacatc
     1201 ccctgcatga ccgtctgctc gtcgccgccg acctcggcga cgccggccgt gcacatctcg
     1261 gcgaggtcgg ggatcatcgc cagccggccg gcgaacgtca cgcggtgcag agcaccccac
     1321 cgccacgcgg cgatgtcgtc gccgaagcgc tgcgtgagct cgtcgagcgc cgcgtcgagc
     1381 gcgtcgcgta gcgccccgtc gcgaccctcc gcgccgtcgc gcccgaacca ccgtgcggtc
     1441 gggtagcgca ggagcgtggg caggacctgg tactggaacg cgttcgtcca ctcgcggcgc
     1501 ccgtggaagt gcgtgaagag gtcggcgtcc atcatcgggc gcaggacggc gcgcgcgacg
     1561 cgctcgcacc agacctcgta gatcgcggcc gccgaggagc ccgccgcgac gtcgccgtcc
     1621 cactcggcga gcaccgcgag cgcgtgcttc tgccggtcgt ccctcggctc gagcgcgacg
     1681 aggaacggga gcatctcgcg tgccgggagc gagaccgtgt cgagctggat cctccggaac
     1741 gtgtccgccg tgtgatgcgg ctcctcggtc acgagctgcg cgatgcgcct cgcacggaac
     1801 ggcgggagga agtcgcggct gatgacgtgg gggtagtcgt cgccgtggat cttgttgttt
     1861 gccgtgacca ggaacccttc ctccgggttg tacgcccgtg ggagctcgtc gtacgggacc
     1921 cagccgtccc attcgtagtc ctcggtccat cccggaaccg gcacggtgcc gtcgcccgtg
     1981 cggcggaccg gatagagccc accgagctgg tatccgatgt tcccgtgggt gtcggcgtag
     2041 accgcgttct gtccggggca ccgccatccc tcgaacgccg cgcggaactc ctcccagtcg
     2101 cgcgcggagt ccacggcgag cagcgtcgac ggctcgacgc tggcgtcggc gccgacccat
     2161 cgc
//
LOCUS       DASZOB010000038         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_20936, whole genome shotgun sequence.
ACCESSION   DASZOB010000038 DASZOB010000000
VERSION     DASZOB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20936"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="phoU"
                     /locus_tag="VG709_00600"
     CDS             complement(<1..615)
                     /gene="phoU"
                     /locus_tag="VG709_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWC31608.1"
                     /translation="MRIMLQADLDQLEAMLHEEGELVLRSLRGALNALVQQDVALADE
                     VIAFDDEVDERYLAIEEGIQSLLARQTPVAVDLRLVLAVLHDNLHLERTADYCVTIAK
                     LTKLVHEAEPDEELVRGFEEMGSRAEEMIRVALDSFVRRDAERPEALVELDELIDKGN
                     RHVVQRVLELGGDPDRREWGLRMIVASRCLERIGDHAVDIGERTA"
     gene            complement(618..1475)
                     /gene="pstB"
                     /locus_tag="VG709_00605"
     CDS             complement(618..1475)
                     /gene="pstB"
                     /locus_tag="VG709_00605"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWC31609.1"
                     /translation="MTESAPVRLPAVTRETPDHESTSSAAREPLFSVARLSVSYGSVA
                     ALGEVSLDVYRNYVTAFIGPSGCGKSTFIRCFNRMNDLIPSARVKGSVLYHDVDLYGA
                     GVDPVEVRKRIGMVFQKPNPFPKSIYDNVAFGPRVLGMKDALDDRVERTLRRAALWDE
                     VKDRLKDSALTLSGGQQQRLCIARCLAVEPDVILMDEPASALDPISPAALEDLMHGLK
                     RDYTIVIVTHNMQQAARVADMTAFFSVRVSDDARNRTGVLVEYDETPTIFTKPSNKRT
                     EDYVTGRFG"
     gene            complement(1481..>1831)
                     /locus_tag="VG709_00610"
     CDS             complement(1481..>1831)
                     /locus_tag="VG709_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWC31610.1"
                     /translation="VRQGAYALGATQWQVIWRQVLPAAGPGIATGSILALARAIGETA
                     PLLLVGALTYVAFDPPLLGDVTALPIQIFQWIARPQEEFRLLAAAGIIVLLGLLLTMN
                     AAAIWLRNRYQRRW"
BASE COUNT          277 a          613 c          650 g          291 t
ORIGIN      
        1 cgcggtccgc tcgccgatgt cgaccgcgtg gtctccgatc cgctcgaggc agcgagaggc
       61 cacgatcatg cggagccccc attcgcgccg gtcggggtcg ccgccgagct cgaggacgcg
      121 ctgcacgacg tgccggttgc ccttgtcgat cagctcgtcg agctcgacga gcgcctcggg
      181 ccgttccgcg tctcggcgca cgaaggagtc gagcgcgacc cggatcatct cctccgcccg
      241 tgagcccatc tcctcgaagc cccggacgag ctcctcgtcc ggctcggcct cgtgcacgag
      301 cttcgtgagc ttcgcgatcg tgacgcagta gtcggccgtc cgctcgagat gcaggttgtc
      361 atggagcacc gcgaggacga gcctgagatc gaccgcgacg ggcgtctgtc tcgcgaggag
      421 cgactggatg ccctcctcga tggcgagata gcgctcgtcc acctcgtcgt cgaacgcgat
      481 cacctcgtcg gcgagcgcga cgtcctgctg gacgagcgcg ttgagcgccc ctcggagcga
      541 cctgaggacg agctcgccct cctcgtggag catcgcctcg agctggtcga ggtccgcctg
      601 gagcatgatc cgcatggcta cccgaatctc cctgtcacgt aatcctcggt gcgcttgttg
      661 gacggcttcg tgaagatcgt gggcgtctcg tcgtactcga cgaggacgcc ggtccggttt
      721 ctcgcgtcgt cggagacacg gacgctgaag aacgccgtca tgtcggcgac gcgcgccgcc
      781 tgctgcatgt tgtgcgtcac gatcacgatc gtgtagtcgc gcttgaggcc gtgcatgagg
      841 tcctcgagcg cggccggcga gatcgggtcg agcgccgagg cgggctcgtc catcaggatg
      901 acgtcgggct cgacggcgag gcagcgcgcg atgcagaggc gctgctgctg tccgccggag
      961 agggtgagcg cgctgtcctt gaggcgatcc ttcacctcgt cccagagcgc ggcccggcgg
     1021 agcgtccgct cgacgcggtc gtcgagggcg tccttcatgc cgagcacgcg cggcccgaag
     1081 gccacgttgt cgtagatgga cttcgggaac gggttcggct tctggaacac catgccgatg
     1141 cgctttcgca cctcgacggg atcgacgccg gcgccgtaga gatcgacgtc gtggtagagg
     1201 acgctgccct tcacccgcgc gctcggaatg aggtcgttca tccggttgaa gcagcggatg
     1261 aaggtgctct tcccgcaacc cgatgggccg atgaacgcgg tcacgtagtt gcggtagacg
     1321 tcgagcgaga cctccccgag cgcggccacg gacccgtacg agacgctcag gcgcgcgacg
     1381 gagaagagcg gctcacgggc ggcggaggac gtcgactcgt gatcgggcgt ctcccgcgtc
     1441 accgcgggca ggcgtacggg cgcgctctcg gtcatcgaca tcaccatctc ctctggtagc
     1501 ggttgcggag ccagatggcc gccgcgttca tcgtcaggag cagcccgagc aggacgatga
     1561 tccccgcggc ggcgagcagc cggaactcct cctgcgggcg cgcgatccac tggaagatct
     1621 ggatcggaag cgccgtgacg tcgccgagga gcggcgggtc gaaggcgacg taggtgagcg
     1681 cgccgacgag aagcagcggg gccgtctcgc cgatcgcccg cgcgagcgcg aggatggagc
     1741 cggtcgcaat cccggggccc gcggccggga gcacctggcg ccagatcacc tgccactgcg
     1801 tcgccccgag ggcgtaggca ccctggcgca c
//
LOCUS       DASZOB010000039         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_20950, whole genome shotgun sequence.
ACCESSION   DASZOB010000039 DASZOB010000000
VERSION     DASZOB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20950"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VG709_00615"
     CDS             complement(<1..453)
                     /locus_tag="VG709_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWC31611.1"
                     /translation="MKVEAPRGTLDVLPAEQPLRDTIVGAAEGAAARYGYGRIVTPVF
                     ENTELFARTSGAGSDVVQKEMYTFPDRAGRSLTLRPEGTAAVARAYLQHGLHREPQPV
                     KTFYVAPMYRYAAPQKGRYREFWQIGFEAMGSDDPAIDAELIQLFVSIE"
     gene            complement(450..1091)
                     /locus_tag="VG709_00620"
     CDS             complement(450..1091)
                     /locus_tag="VG709_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC31612.1"
                     /translation="MALDVARLELGPIGTNCYLVRRPGAAEAVVVDPGASASENRIEL
                     AGRGARCVAILLTHGHWDHLGGVADLAEGTGAPVHMAEDERALLEHVNDYVPPGVRLR
                     PWEPEVLLQGDETLELAGIAFETLRVPGHSPAHLAYAADGALFSGDVLFAGSVGRTDL
                     PGGDWDALLASLRSLADRFPAETVVYSGHGPPTTLAHELATNPFLSELRATNR"
     gene            1138..1482
                     /locus_tag="VG709_00625"
     CDS             1138..1482
                     /locus_tag="VG709_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWC31613.1"
                     /translation="MRSWRLPEIDTPDGSRSPVVLLSEDEARAVLIGLHPGQELGDHQ
                     VKEHAWVVVVHGSVEIGTGGETIEAGVGTLVHFEPDERHFVRSTEGAKFLLLLAPWPG
                     AGHYRGQEKDRR"
     gene            complement(1807..2223)
                     /locus_tag="VG709_00630"
     CDS             complement(1807..2223)
                     /locus_tag="VG709_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31614.1"
                     /translation="MPSTKQRRRRQKLQRHEYEYVVETEEGEVPVERLADVERRPKVK
                     DGRKGSAPALVDRRGRPIRKPSLGRVLKRTAIFAPILLLFVYLAGPQSVEPVVMVFNT
                     LLLVAFFIPFSYFVDTMVYRLILRRYEREQAEGGRG"
BASE COUNT          349 a          797 c          809 g          332 t
ORIGIN      
        1 ttcgatggag acgaagagct ggatgagctc ggcatcgatc gccgggtcgt cggagcccat
       61 cgcctcgaat ccgatctgcc agaactcgcg atagcgcccc ttctggggcg ccgcgtagcg
      121 gtacatcggt gcgacataga acgtcttcac cggctgcggc tcgcgatgaa ggccgtgctg
      181 gaggtacgcg cgcgcgaccg cggccgtccc ttcggggcgg agagtgagcg atcgcccggc
      241 ccggtcgggg aacgtgtaca tctccttctg gacgacgtcc gaaccggctc ccgacgtccg
      301 ggcgaagagc tcggtgttct cgaacaccgg cgtcactatg cggccgtatc cgtaccgggc
      361 ggccgcgccc tccgccgcgc cgacgatcgt gtcgcggagc ggctgctcgg cgggaaggac
      421 gtcgagcgtt ccccgcggtg cctcgacctt caccggttgg tcgctcgaag ctcggagagg
      481 aacgggttcg tggcgagctc gtgcgcgagc gtggtgggcg gcccgtgccc cgagtagacg
      541 acggtctcgg ccggaaacct gtccgcgagc gagcggaggg acgcgaggag cgcatcccag
      601 tcgccgcccg gaagatccgt ccggcccacc gagccggcga agagcacgtc gcccgagaac
      661 agcgcgccgt ccgccgcgta ggcgaggtgc gcgggcgagt gccccgggac gcgaagcgtc
      721 tcgaacgcga tccccgcaag ctcgagcgtc tcatcgccct gcaggaggac ttcgggctcc
      781 cacggacgca ggcggacgcc cggagggacg tagtcgttca cgtgctcgag gagggcccgc
      841 tcgtcctctg ccatgtggac gggagcgccc gtgccctcgg cgaggtccgc gacgccgccc
      901 aggtggtccc agtggccgtg tgtgagcagg atcgcgacgc agcgggcgcc gcggcccgcg
      961 agctcgattc ggttctcact cgcgctcgca ccggggtcga cgacgacggc ttccgccgcg
     1021 cccggccggc gaacgaggta gcagttcgtg ccgatggggc ctagctcgag ccgtgcaacg
     1081 tcgagggcca tcaagcgcct gagtctaacg ggggcctggt tagactccgg cgcgcccatg
     1141 cgaagctggc ggctgcccga gatcgacacg ccggacggca gccgctcgcc cgtcgtcctg
     1201 ttgagcgagg acgaggctcg cgccgtcctc atcggcctcc atccaggcca ggagctcggc
     1261 gaccaccagg tcaaggagca cgcctgggtc gtcgtcgtgc acggatccgt cgagatcggc
     1321 accggaggcg agacgatcga ggcgggggtc gggacgctcg tccacttcga gcccgacgag
     1381 cggcacttcg tccggagcac cgagggcgcg aagttccttc tcctgctggc cccgtggccg
     1441 ggcgcgggcc actaccgagg acaggaaaag gatcgaaggt gagcgagcgc tcggccggcg
     1501 agccacgtcg atgcgacgtg ctcgcggccc ggcgcctctc tcgcgcaacc ttcccgccga
     1561 cggtaacggc ccgcgggtgg gacggatgct ggtgcaatcg cgtgacgcac gcggcacgat
     1621 gcgtatcact cggctgcgac tcttcgagct acgctcgccg cgggggaacc gctaaccggg
     1681 ggtccccggg ggaatcacgc gcgtaccggc tagggcgtcc tatctcgccg acgcgatgcg
     1741 cgactctgac tccgaccaca cgcggtgtca ctgccggcgc ggaagccgac cgctgagaga
     1801 agcgccttag ccgcggcctc cctccgcctg ctcgcgctcg tagcggcgca ggatcaggcg
     1861 gtacaccatc gtgtcgacga agtagctgaa cgggatgaag aacgcgacga gcaggagcgt
     1921 gttgaacacc atcacgacgg gctcgacgct ctgcggtccc gccaggtaga cgaagagcag
     1981 aagaatcggc gcaaagatcg cagtccgctt cagcacgcgt ccgagcgacg gcttccggat
     2041 cggacggccg cggcggtcga cgagcgccgg cgcggatccc ttccgaccgt ccttcacctt
     2101 cggccggcgc tccacgtccg cgagccgctc gaccgggacc tcgccctctt ccgtctcgac
     2161 gacgtactcg tactcgtgcc gctggagctt ctgccgtcgc cgccgctgct ttgtgctcgg
     2221 cataccgtga ttatgcccct ggctcggcgc ccgcggcgga gacgacctcg ccgttcttga
     2281 ccacgcc
//
LOCUS       DASZOB010000040         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_162375, whole genome shotgun sequence.
ACCESSION   DASZOB010000040 DASZOB010000000
VERSION     DASZOB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162375"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VG709_00635"
     CDS             complement(<1..224)
                     /locus_tag="VG709_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31615.1"
                     /translation="MLLTVAAATYAVSGVAGAFDRELLGVFDALMVVVLGLVLYSMSA
                     RDPSRPAGVMDRVTLVAVASALVLDVMVLGA"
     gene            246..737
                     /locus_tag="VG709_00640"
     CDS             246..737
                     /locus_tag="VG709_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31616.1"
                     /translation="MLTMLFTPLFAVMLTVAAITYAVSGVAGAFDRELLGVFDALMVV
                     VLGLVLYAMSARAPARPVGLMDRVQLVAVASALVLDVMVLGAMVARIGDLGFTPNRVA
                     ALGLNLVLLVNLAGTAWLSMRFLTGRIAFHRLERWQTSYLPIFALWAATVVAVLPPVF
                     AFT"
     gene            complement(826..1257)
                     /locus_tag="VG709_00645"
     CDS             complement(826..1257)
                     /locus_tag="VG709_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HWC31617.1"
                     /translation="MAAEKQAQVTWRGDLATGGGSVDSVGSGSFGPLAVSWPKRTGEE
                     EGTSPEELIAAAHAACFSMAFSKRLADAGATPENLSVSARVTFVPGTGITTSALEVVG
                     TAPGIDDDAFRQAAEDAKENCPVSQALTGNVEMTVDARLSG"
     gene            1372..>1736
                     /locus_tag="VG709_00650"
     CDS             1372..>1736
                     /locus_tag="VG709_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31618.1"
                     /translation="MDLRVRPFTLALTFTVVGALTVWPSSTAVAVPRCLGKPATIVGT
                     PGNDTLVATNGNDVIVAGGGDDMIRARGGRDRICAKGGADTMRGGPGNDRMDGGFGPD
                     VFFGDDGSDWAMYPSRVTKV"
BASE COUNT          255 a          620 c          587 g          274 t
ORIGIN      
        1 gcgccgagca ccatcacgtc gagcacgagg gcgctcgcga cggcgaccag cgtgacgcgg
       61 tccatgacgc cggccggtcg agaaggatcg cgcgccgaca tggagtagag gacgagcccc
      121 agcacgacca ccatgagggc gtcgaagacg ccgagcagct cgcggtcgaa cgcgccggcg
      181 actcccgaca ccgcataggt ggccgcggcg acagtcagca gcacgtcgtg gagaacatgg
      241 cgccggtgct caccatgctc ttcacgccgc tgttcgccgt gatgctgacg gtcgctgcga
      301 tcacgtacgc cgtgtccggc gtcgccggcg cgttcgaccg cgagctgctc ggcgtcttcg
      361 acgccctgat ggtggtcgtg ctggggctcg tcctctacgc gatgtccgcg cgcgccccgg
      421 cccgaccggt cgggctgatg gaccgcgtcc agctggtcgc cgtcgcgagc gccctcgtgc
      481 tcgacgtgat ggtgctcggc gccatggtcg cccggatcgg agatctcggc ttcaccccga
      541 accgcgtggc ggcgctcggc ctcaacctcg tcctgctcgt gaacctggcc gggaccgcgt
      601 ggctttcgat gcggttcctc acgggacgga tcgccttcca ccgcctggag cgatggcaga
      661 cgtcctacct ccccatcttc gccctttggg cagcgacggt cgtcgccgtt ctcccgcccg
      721 tcttcgcctt cacgtgatct gctcccttgg agatccggtt cgtctccacg acgaacgaga
      781 accgcgttag atccacaccg cccgaaggga aaacgagcgg aggcctcagc cgctcagacg
      841 cgcgtcgacc gtcatctcga cgttgccggt gagggcctgg gagacggggc agttctcctt
      901 cgcgtcctcc gcggcctgtc ggaacgcgtc gtcgtcgatg cccggagccg tcccgacgac
      961 ctcgagcgca gaggtcgtga tcccagtgcc cgggacgaac gtcacgcgcg cggagacgga
     1021 gaggttctcc ggcgtggcgc ccgcgtcggc gagcctcttc gagaacgcca tcgagaagca
     1081 ggcggcgtgg gccgccgcga tgagctcctc cggactcgtg ccctcctcct cgccggtgcg
     1141 cttcggccac gagacggcga gtggcccaaa cgagccgctc ccgacgctgt cgaccgaacc
     1201 tcccccggtc gccaggtcgc ctcgccacgt cacctgtgcc tgcttctcgg ctgccatcgt
     1261 tccctcctgt gtgggtcgtc gacgagtcaa cttacccgtc gctccgacct tgaccctgtg
     1321 atcgcagcgg cggacaatcc gcgggcacac tcctcgggga aagggcggag catggacctg
     1381 cgcgtacgac ccttcacgct cgcactcacg tttaccgtcg tgggagcgct gaccgtatgg
     1441 ccgtcgtcga cggcggttgc cgtgcctcgc tgcctcggga agccggcgac gatcgtcggc
     1501 acgccgggga acgacacact ggtcgcgacc aatggaaacg acgtcatcgt ggcgggaggc
     1561 ggcgacgaca tgatccgcgc ccgcggcggc cgggaccgga tctgcgcgaa aggcggcgcc
     1621 gacacgatgc gcggcggccc agggaacgac cggatggacg ggggcttcgg gcccgacgtt
     1681 ttcttcggcg acgacggcag cgactgggcg atgtacccga gccgcgtcac caaggt
//
LOCUS       DASZOB010000041         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131694, whole genome shotgun sequence.
ACCESSION   DASZOB010000041 DASZOB010000000
VERSION     DASZOB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131694"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VG709_00655"
     CDS             <1..358
                     /locus_tag="VG709_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31619.1"
                     /translation="AARVDDRLVEPMDGRSLLRSWNRPWILTEFWPGTRLDVPAWASI
                     RAPDRQYVEYYDDTRVRPVFREYYDLERDPRQRRNKLAGPVWSMRASPAETTRMSLEL
                     AVARSCRGTAPPRECP"
     gene            409..1770
                     /locus_tag="VG709_00660"
     CDS             409..1770
                     /locus_tag="VG709_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWC31620.1"
                     /translation="MRLLLPSLTLPILLASGLSSVHPARSASAQPLLRPNILIVMTDD
                     QRAGGTMNVMPKTREWFGESGTRFTRAFVSTPTCCPARASIYTGRYTHNHGVRTNRSI
                     DELDHGSTLQRYLQRAGYRTAFAGKFFNFWDLELSPPYFDRWAVFKGTRYYDVRVNAD
                     GSQERVPDYITTFVARYATDLIRDDFENDDLAPWLLYVAPLAPHMRATPEPRYRGAPV
                     GRWRGNPAVRERDERDKPRYVRRIDHEIAEARRIRTRQLRTLLSVDDLVDEVFRTLED
                     TGELSNTLAFFVSDNGFMWAEHGLLGKSVPYTEAIRVPMMMRRPGLEAVPARDDRLAS
                     LVDIAPTALEAAGVVPDREFPIDGRSLLQPDERGFLLVENDARRWASIRTRRYQYIEH
                     FQRDGTGTTFSEYYDLRRDPWQLVNLFRDGDRTNDPDWQPLHRRLRRATACAGTEPAG
                     NCP"
     gene            1794..>3306
                     /locus_tag="VG709_00665"
     CDS             1794..>3306
                     /locus_tag="VG709_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWC31621.1"
                     /translation="MGDDLERRSTDSGIEIRPLYEAGDLSAFDPERDLGRPGEPPFTR
                     GPYPSMYRGRVWTMRQYAGMGTAEETNGRFRYLLDRGQTGLSVAFDLPTQMGLDSDHP
                     RATDEVGRTGVAIDSVEDMERLLDGIPLDQVTTSMTINATAAILLLLYELVAEEQGVP
                     PEKLGGTVQNDILKEYAARGTYIYPPKPSMRLVTDLFAYCRDRVPRWNTISISGYHMR
                     EAGSTAVQEVAFTLANAIAYVQAALDAGLGVDQFGPRLSFFFACHMNFFEEAGKFRAA
                     RRMWSRIMTERFGAKDPRSAMLRFHTQTGGATLTAQQIENNIVRTALEGLAAILGGTQ
                     SLHTNSFDEALALPTERAAKIALRTQQVLAYESGVADTVDPLAGSYFVETLTNEIETR
                     AWEYLEKIDGMGGAVAAIEAGWIQDEIHESAYRIQQRVESGERAIVGVNRYVEDDEEP
                     VELQRIDEAQVRRQAERLRELRERRDNAAVERALGEVEETARGTGNLLVPMRAA"
BASE COUNT          528 a         1139 c         1176 g          463 t
ORIGIN      
        1 cgcggcccgc gtggacgacc ggctcgtcga gccgatggac ggccggtcgc tgctccgaag
       61 ctggaacagg ccgtggatcc tgaccgagtt ctggcccggg acccggctcg acgtcccggc
      121 gtgggcctcg atccgcgccc ccgaccgtca gtacgtcgag tactacgacg acacgagggt
      181 ccgcccggtg ttccgcgagt actacgacct cgagcgcgac ccgcggcagc ggcgtaacaa
      241 gctcgccggc cccgtctggt cgatgcgggc ctctcccgcg gagacgacga ggatgtcgct
      301 ggagctcgcc gtcgcgcggt cgtgccgggg gaccgctccc cctcgggagt gcccttgagc
      361 gccccgactg cggtcggcac ggcggcgtcc tcgtagactc gacggtccat gcgcctcctg
      421 cttccctcgc tcacgttgcc gatcctcttg gcgtccgggt tgtcgtcggt tcacccggcg
      481 cgatcggcgt cggcacagcc gctgctgcgc ccgaacatcc tcatcgtgat gaccgacgac
      541 caacgcgccg gcggcacgat gaacgtgatg ccgaagacgc gggagtggtt cggggagagc
      601 gggactcgct tcacccgagc gttcgtgtcg acgccgacgt gctgcccggc acgcgcgtcg
      661 atctacacgg gccggtacac gcacaaccac ggcgtgcgga cgaatcggag tatcgacgag
      721 ctcgatcacg ggtcgacgct ccagcgctac ctgcagcggg ccggataccg aaccgcgttc
      781 gcggggaagt tcttcaactt ctgggacctc gagctgtcgc ccccctactt cgaccgatgg
      841 gcggtgttca aggggacgcg ctactacgac gtgcgcgtca acgccgacgg atcgcaggag
      901 cgcgtccccg actacatcac gacgttcgtc gcgcggtacg cgaccgacct gatccgcgac
      961 gacttcgaga acgacgacct ggccccgtgg ctgctgtacg tggcgccgct cgcgccccac
     1021 atgcgagcga cgccggagcc gaggtaccgg ggtgcgccag tcggccggtg gcgcgggaac
     1081 ccggccgtac gcgagcgaga cgaacgcgac aagccacgat acgtgcgacg gatcgaccac
     1141 gagatcgccg aggcccgtcg catcagaacg cgtcagctac ggacgctgct gtcggtcgac
     1201 gacctggtgg acgaggtgtt ccggacgctc gaggacaccg gggagctctc gaacacgctc
     1261 gcgttcttcg tctccgacaa cgggttcatg tgggcggagc acgggctcct cgggaagtcg
     1321 gtgccgtaca cggaggcgat ccgcgtgccg atgatgatgc gccggcccgg gctcgaggcg
     1381 gtgcccgcac gcgacgacag gctcgcgtcg ctcgtcgaca tcgcgccgac ggcgctggag
     1441 gcggcgggcg tcgtgccgga ccgcgagttc ccgatcgatg gacgctcgct gctccagccc
     1501 gacgagcgag gtttcctcct cgtcgagaac gacgcacggc gttgggcgtc gatcaggacg
     1561 cggaggtacc agtacatcga gcactttcag cgcgatggga ccgggacgac gttctcggag
     1621 tactacgacc tccgacgcga cccgtggcag ctcgtgaacc tcttccgcga cggggaccgc
     1681 acgaacgacc cggactggca gccgctgcat cggcggctcc ggcgagccac ggcatgcgcg
     1741 gggacggagc cggccgggaa ctgcccgtga cggggcggta gcctgtaccc cgcatgggcg
     1801 acgacctcga gcgccgcagc acggactccg ggatcgagat ccggccgctc tacgaggcgg
     1861 gcgatctctc ggcgttcgat cccgagcgcg acctcggccg gcccggcgag ccgccgttca
     1921 cgcgcggccc gtacccatcg atgtaccggg gacgcgtctg gacgatgcgc cagtacgccg
     1981 ggatgggaac cgcggaggag acgaacggcc ggttccgcta cctcctcgac cgcgggcaga
     2041 cggggctgtc cgtcgcgttc gacctgccca cgcagatggg gctcgactcc gaccacccgc
     2101 gcgcgaccga cgaggtaggg cgcaccggcg tcgcgatcga ctcggtcgag gacatggaac
     2161 ggctcctcga cggcataccg ctcgaccagg tcaccacctc gatgacgatc aacgcgacgg
     2221 cggcgatcct gctgctgctc tacgagctcg tcgccgagga gcagggcgtg ccgccggaga
     2281 agctcggcgg caccgtgcag aacgacatcc tcaaggagta cgcggcccgc gggacataca
     2341 tctacccgcc gaaaccgtcg atgcggctcg tcacggacct gttcgcgtac tgccgcgacc
     2401 gggtcccgcg ctggaacacc atctcgatct ccgggtacca catgcgcgag gcggggtcga
     2461 cggccgtcca ggaggtcgcg ttcacgctcg cgaacgcgat cgcgtacgtg caggccgcgc
     2521 tcgacgccgg tctcggcgtc gaccagttcg ggccgcggct ctcgttcttc ttcgcctgcc
     2581 acatgaactt cttcgaggag gcggggaagt tccgcgcggc gcggcggatg tggtcgcgga
     2641 tcatgaccga gcgcttcgga gcgaaggatc cgcgatcggc gatgctgcgc ttccacacgc
     2701 agaccggcgg ggcgacgctc acggcgcagc agatcgagaa caacatcgtc cgtacggcgc
     2761 tcgaggggct cgccgcgatc ctcggcggca cgcagtcgct gcacacgaac tcgttcgacg
     2821 aggcgctggc gctgccgacg gagcgcgccg ccaagatcgc gctccggacg cagcaggtgc
     2881 tcgcgtacga gtcgggcgtc gcggatacgg tcgacccgct cgcgggctcg tacttcgtcg
     2941 agacgctcac gaacgagatc gagacccgcg cgtgggagta cctcgagaag atcgacggga
     3001 tgggcggagc ggtggcggcg atcgaggccg gttggatcca ggacgagatc cacgagtccg
     3061 cgtaccgcat ccagcagcga gtcgagtcgg gggagcgcgc catcgtcggg gtcaaccgct
     3121 acgtcgagga cgacgaggag cccgtggaac tccagcggat cgacgaggcg caggtgcggc
     3181 gccaggccga gcggctgcgc gagctgcggg agcgtcgcga caacgccgcg gtcgagcgcg
     3241 cgctgggcga ggtcgaggag acggcgcgtg gcacggggaa cctcctcgtt ccgatgcgcg
     3301 cggccc
//
LOCUS       DASZOB010000042         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10518, whole genome shotgun sequence.
ACCESSION   DASZOB010000042 DASZOB010000000
VERSION     DASZOB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10518"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1989
                     /locus_tag="VG709_00670"
     CDS             <1..>1989
                     /locus_tag="VG709_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HWC31622.1"
                     /translation="DDYVVTWAVGHLVELAQPEDYDERFKKWRMADLPIVPDAFKLKP
                     RDAKAKKQLSVIHKLLKRDDVERVINACDSGREGELIFAYIWETAGVDKPVERLWINS
                     MTKQAIREGFEALKPGAQMEPLELAARSRSEADWLVGMNATRAATLRGRAWVGGVVSL
                     GRVQTPTLAMMVSRERQIQAFVPEPYWLVHGTFEAPSRAGYEGLWFEGDETRLKDGKR
                     AEEIVDRVSGKDGTVASVERKEQSERAPLLYDLTSLQREANRRFGFSARRTLQAAQSL
                     YEDKKAITYPRTNSRYLSGDMVAQLKPTAATLVPIREYAPHARFVLGLEKLPLARVVN
                     DERVDDHHAIIPTDIDHDVDGFRDDERRIFDLVAKRFLAVFHPAARYERTTVVTEVEG
                     ERFRTRGKVTLEAGWRGVYGVEADDRAGTAPDEDAETGELPRLEEGMTVRCAEAVSEA
                     KETKPPPRYTEATLLSAMETAGKLIDDEELREAMKERGLGTPATRAEIIETLIRREYI
                     ERVAKDLVPTPKGLQVISMIEEHPLTSPELTGDWEKRLTDIEHGAGDRSEFMGGIERL
                     ARATVEEIAALDREKLRPERAELGLCPRCGAETGEIIRENSRAFGCTSWKSREEPGCG
                     FVIWKRVAGRTLTPEIARQLLEEGRTRDVLSGFRSRNKK"
BASE COUNT          337 a          694 c          712 g          246 t
ORIGIN      
        1 gacgactacg tcgtcacctg ggcggtcggc cacctggtcg agctcgccca gccggaggac
       61 tacgacgagc ggttcaagaa gtggcggatg gccgacctcc cgatcgtccc ggacgccttc
      121 aagctgaagc cgcgtgacgc gaaggcgaag aagcagcttt ccgtcatcca caagctcctg
      181 aagcgcgacg acgtcgagcg cgtcatcaac gcgtgcgact ccggccgcga gggcgagctc
      241 atcttcgcgt acatctggga gacggccggc gtcgacaagc ccgtcgagcg gctctggatc
      301 aactcgatga cgaagcaggc gatccgggag gggttcgaag cgctcaagcc gggcgcgcag
      361 atggagccgc tcgagctcgc ggcgcggtcc cgctcggagg cggactggct cgtcgggatg
      421 aacgcgaccc gggcggcgac gctccgcggc cgcgcctggg tcggaggcgt cgtctcgctc
      481 ggccgcgtcc agaccccgac gctcgccatg atggtaagcc gcgagcgcca gatccaggcg
      541 ttcgtcccag agccgtactg gctcgtgcac gggacgttcg aggcgccgtc gcgcgcgggc
      601 tacgagggcc tctggttcga gggcgacgag acgcgtctca aggacgggaa gcgcgccgaa
      661 gagatcgtcg atcgcgtctc gggcaaggac gggaccgtcg cctccgtcga gcgcaaggag
      721 cagtcggagc gcgcgccgct cctctacgac ctcacgagcc tccagcggga ggcgaaccgc
      781 cgcttcggtt tctccgcgcg ccgcaccctc caggcggcgc agtcgctcta cgaggacaag
      841 aaggcgatca cgtacccgcg tacgaactcg cgctacctct ccggcgacat ggtcgcgcag
      901 ctcaagccga cggccgcgac gctcgtcccc atccgcgagt acgcgccgca cgcgcggttc
      961 gtgctcgggc tcgagaagct gccgctcgcc cgcgtcgtca acgacgagcg cgtcgacgac
     1021 caccacgcga tcatccccac cgacatcgac cacgacgtcg acggcttccg cgacgacgag
     1081 cggcgcatct tcgacctcgt cgccaagcgc ttcctcgcgg tgttccaccc ggccgcacgc
     1141 tacgagcgga cgaccgtcgt caccgaggtc gagggcgagc gtttccggac gcgcggcaag
     1201 gtgacgctcg aggccggctg gcgcggcgtc tacggggtcg aggccgacga ccgcgcaggg
     1261 acggcaccgg acgaggacgc ggagaccggc gagctgcccc gcctcgagga ggggatgacc
     1321 gtccgctgcg ccgaggccgt gagcgaggcg aaggagacga agccgccgcc acggtacacc
     1381 gaggcgacgc tcctctcggc gatggagacg gccgggaagc tgatcgacga cgaggagctc
     1441 cgcgaggcga tgaaggagcg cggtctcggc acgccggcga cccgcgcgga gatcatcgag
     1501 acgctcatcc ggcgcgagta catcgagcgc gtcgccaagg atctcgtccc gacgccgaag
     1561 ggcctgcagg tgatctcgat gatcgaggag catccgctca cgtcgcccga gctcaccggc
     1621 gactgggaga agcgcctgac cgacatcgag cacggcgcgg gcgaccgctc cgagttcatg
     1681 ggcgggatcg agcggctcgc gcgggcgacg gtcgaggaga tcgccgcgct cgaccgcgag
     1741 aagctgcgcc ccgagcgggc cgagctcggc ctctgcccgc gctgcggcgc cgagaccggc
     1801 gagatcatcc gcgagaactc gcgcgcgttc ggctgcacga gctggaagag ccgcgaggag
     1861 cccggctgcg gtttcgtgat ctggaagcgc gtcgccggcc ggacgctcac gcctgagatc
     1921 gcgcgccagc tcctcgagga gggaaggacc cgcgacgtcc tctccggctt ccggagccgc
     1981 aacaagaag
//
LOCUS       DASZOB010000043         5405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51342, whole genome shotgun sequence.
ACCESSION   DASZOB010000043 DASZOB010000000
VERSION     DASZOB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51342"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..386
                     /locus_tag="VG709_00675"
     CDS             90..386
                     /locus_tag="VG709_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31623.1"
                     /translation="MDQLKKAFYATLGWGDIAAEKSGAWVSRARELATSREETAKSYR
                     QLAMRGERVVKRFSKTPQAERVLGGTKQATRQIKGAYTSVRKALGAEQPRRKAS"
     gene            476..1753
                     /locus_tag="VG709_00680"
     CDS             476..1753
                     /locus_tag="VG709_00680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC31624.1"
                     /translation="MNGDLGGRIALLSLHTSPLDRPGSGDSGGMNVYVREVALGLAAR
                     GVEVDVFTRCSGRGADPVQELAPGARVIDVDAGPCSPVRKEDLPEVVPEFTSELLRSV
                     ERDGGAYDLVQSHYWLSGLVGRAVTELWRTPLVALFHTLARVKNLSVRDGEAAEPESR
                     AEGEELVARHASRVVVPTATEAGYLSELYGVEQERVRLAPPGVDTTRFRPLEKAAARR
                     RVGLDPGARVALVAGRLQALKAPDVAIRALAEARARGPADAAPLVLAIVGGPSPSAGS
                     HDVARLRSEAEALGVADRVVFVEALDHDEMPWVYSAADVVLMPSRSESFGLVALEAQA
                     CGVPVVASSVGGLRHNVVDGKTGFLVPPGDAAALGDGMLAVISDDRTRARMSEAARRR
                     AEGFSWPSTVSRLVRVYAEVRPDVWSVEARQPA"
     gene            2202..2741
                     /locus_tag="VG709_00685"
     CDS             2202..2741
                     /locus_tag="VG709_00685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWC31625.1"
                     /translation="MPGPVLLVIDTQEGFTRKGSLASEHTTAAIPRIVHVAERERGAG
                     TPIIFTKDTHREGDPEFEMFPPHCLEGTEEHELVEELRPLERDAHEIVHKSRYSAFHG
                     TDLDALLEKLDPDEVRVAGVCTDICVLHTVADLRNRDYRVLVHRDAVETFDAPGHDHA
                     DVNRWALSHIENILGAQVV"
     gene            2746..3675
                     /locus_tag="VG709_00690"
     CDS             2746..3675
                     /locus_tag="VG709_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWC31626.1"
                     /translation="MSGPPLRGPILWTTARPAVRRFVTESRLGRRVALRFVAGETLEA
                     AIETAERLGGEGIRSMLDHLGENVASQPQCASAADHYVLALKRIEERPRLECNIAIKL
                     TQLGLDLSTELCVENAERVLQAAAARGTVVMIDMESHEYVDRTLELLRLLRRRYDGVG
                     VALQSYLYRTADDIFSLPEGTPVRLVKGAYLEPPDVAFPKRADVDRSYARLFATLASR
                     GHEVHVATHDPTLLAGAAGFVERRGIPWERIELQMLYGIRTDLQREHARRGHPVRTYI
                     PYGEEWYPYLTRRLAERPANVWFFASHVLRRGT"
     gene            3672..4502
                     /gene="proC"
                     /locus_tag="VG709_00695"
     CDS             3672..4502
                     /gene="proC"
                     /locus_tag="VG709_00695"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWC31627.1"
                     /translation="MNERLVAFLGTGKMGEALVSGLIRSGGRTSEEIIVTARREERAR
                     ELADRYGLTATLSNAEAIEWAPTMILTVKPQDMGALLDQIADVVEPKHLVISFAAGIR
                     CASIEARLQPGVPVVRVMSNVPVLVDEAMSAISPGAVAEDKHLAIAQELLGYVGRVVR
                     LAEQHQDAITATSGSGPAYFALLAEAMIDACILLGLSRDVATELIVQTMLGSAKMLRD
                     TGKHPVEIREMVTSPGGTTIAAIRHLEQAGVRAAFLNAIDAARQRSAELAKGSEAEEV
                     "
     gene            4638..>5405
                     /locus_tag="VG709_00700"
     CDS             4638..>5405
                     /locus_tag="VG709_00700"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWC31628.1"
                     /translation="MFPLSKGRVAKQAHVGLPGGTYEEEHGREAFEGRASHLYRSHPP
                     TAWVRIEGHLRPRAYDLNPLKPANGERGDWTRILWNDDVAIYVSMQPVARDWFLRDSD
                     GDLCYFVHRGEGTLETDYGPLPFRAGDFLVVPKGTTHRLVPETQDNFFYVVEGRGEYS
                     VPEKGILGKHALFDPGVLETPEPEPHDEEGEFEVRVKRDDRYTVLTYPFHPIDGVGWQ
                     GDLCPVRLNVEDFRPVGSPRYHLPPAVHVTWKSEGFEI"
BASE COUNT          805 a         1852 c         1994 g          754 t
ORIGIN      
        1 ggcgggaggg tggagtccgc aggctgaccc tagccctcac ccggctcccc tcagccctcc
       61 cgtccccaat cccgaaggag gtgaaacaca tggaccagct gaagaaggcc ttctacgcga
      121 cgctcggctg gggcgacatc gccgccgaga agagcggcgc gtgggtctcc cgggcgcgtg
      181 agctggcgac gtcgcgcgag gaaaccgcga agtcgtaccg ccagctcgcg atgcgcggcg
      241 agcgcgtcgt gaagcggttc agcaagacgc cgcaggccga gcgcgtgctc gggggcacca
      301 agcaggcgac gcggcagatc aagggcgcgt acacgagcgt ccgcaaggcg ctcggcgccg
      361 agcagccgcg gcgcaaggca agctgagcga cgtggagcgg cgacggaggg cccccctcgg
      421 gggccctccg tttgcgttcg ggcaccggcg ggcagactga cgtaccgtgt cggccgtgaa
      481 cggcgacctc ggcggccgca tcgcccttct cagcctgcac acctcgccgc tcgaccgtcc
      541 cgggtcgggc gactccggcg ggatgaacgt ctacgttcgc gaagtcgcgc tcggcctcgc
      601 ggcccgcggc gtggaggtcg acgtcttcac gaggtgctcg gggcgcggcg ccgaccccgt
      661 gcaggagctc gcgcccggcg cccgcgtgat cgacgtcgac gcgggcccgt gctcgccggt
      721 gcggaaggag gatctccccg aggttgtccc ggagttcacc tccgagctgc tccggagcgt
      781 cgagcgcgac ggcggagcct acgacctcgt ccagagccac tactggctgt cggggctcgt
      841 ggggcgggcc gtgacggagc tgtggcgaac gccgctggtc gcgctgttcc acacgctcgc
      901 gcgcgtgaag aacctgagcg tgcgcgacgg cgaggcggcc gagcccgagt cgcgggccga
      961 gggcgaggag ctcgtcgctc gccacgcctc gcgcgtcgtc gtgccgaccg cgaccgaggc
     1021 cggatacctc tcggagctct acggggtcga gcaagaacgc gtgcggctcg ctccgccggg
     1081 cgtcgacacg acgaggttcc gtccgctcga gaaggccgca gcgcgccgtc gggtcggtct
     1141 cgacccgggc gcccgcgtcg cgctcgtcgc ggggcggctc caggctctga aggcccccga
     1201 cgtcgcgatc cgagcgctcg cggaagcccg tgcccgcggg ccggccgacg ccgcgccgct
     1261 cgtgctggcg atcgtcgggg gcccgagccc ctccgccggc tcacacgacg tggcgaggct
     1321 ccgctccgaa gccgaggcac tcggcgtcgc cgaccgcgtc gtgttcgtcg aggcgctcga
     1381 ccacgacgag atgccgtggg tgtactcggc ggcggacgtc gtgctgatgc cgtcgcgctc
     1441 ggagtcgttc gggctcgtcg cgctcgaggc acaggcgtgc ggcgtgcccg tcgtcgcctc
     1501 ctccgtcggt gggctgcggc acaacgtcgt cgatggtaag acgggcttcc tggtgccgcc
     1561 gggcgacgcg gcggcgctcg gcgacgggat gctcgcggtg atctccgacg accgcacgag
     1621 ggcgcggatg tccgaggccg cgcgtcggcg ggccgagggg ttctcgtggc cgtcgacggt
     1681 gtcgcggctc gtgcgcgtct acgcggaggt ccgtcccgac gtgtggagcg tcgaggcgcg
     1741 ccagcccgcc tgacgcaact tgtcactctt cttccggcag cggttttccg cctctgacct
     1801 gctggtttcc gttgtgacgt ggccttttgt ctcacttgac gcgtatcgga cccgtcgcta
     1861 gagtgcgcct cgctccgagc agccaaggtg gctgagtcag ggaaccgaat ggagccccct
     1921 gggaaagccg taaggcggac tgggcgcacc tagtcgtacg gtggcgaagg ccaagtcgct
     1981 gtgcgggtgg ggaggctcgg aacgccccaa agcccaacga aaggggcgat cataaggaaa
     2041 ccgagacagc tgcgaaggtt gctcggggca ctaggcccga ggggctgacg gtggccccgc
     2101 gcggcgtgag gttcgcctca cgtggcgcgg cggcgagccg aaggtccctc gggcctttca
     2161 cgtttcaggc cgcgtcgagc ccccgcccta tactgggatc gatgcctggt ccggtccttt
     2221 tggtcatcga cacccaggaa ggcttcacgc gcaagggcag cctcgcgagc gagcacacga
     2281 cggccgcgat cccgcggatc gtgcacgtcg ccgagcgaga gcgcggcgcc gggacgccga
     2341 tcatcttcac gaaggacacc catcgggaag gcgacccgga gttcgagatg ttccccccgc
     2401 actgcctgga ggggaccgag gagcacgagc tcgtcgagga gctccgcccg ctggagcggg
     2461 atgcgcacga gatcgttcac aagagccggt attcggcgtt ccacggcacg gacctcgacg
     2521 cgctgctcga gaagctcgac ccggatgagg ttcgcgtcgc cggcgtttgc accgacatct
     2581 gcgtgctcca taccgtcgcc gacctccgga accgagacta ccgcgtcctg gtccaccgcg
     2641 acgccgtcga gacgttcgac gcgccgggcc acgaccacgc cgacgtgaac cgttgggcgc
     2701 tctctcacat cgagaacatc ctcggcgcgc aggtggtctg agggcgtgag cgggccgccg
     2761 ctgcgcgggc cgatcctctg gacgacagcg cggcccgccg tccgtcggtt cgtcaccgag
     2821 agccgcctcg gacgccgcgt cgcgctccgg ttcgtggcgg gcgagacgct cgaggcggcg
     2881 attgagacgg ccgagcgcct cgggggcgag ggcatccgct cgatgctcga ccatctgggg
     2941 gagaacgtcg cgtcgcagcc gcagtgcgcg tcggcggccg accactacgt actcgcgctg
     3001 aagcgcatcg aggagcggcc gcggctcgag tgcaacatcg cgatcaagct cacgcagctg
     3061 ggcctcgacc tctcgacgga gctctgcgtc gagaacgcgg agcgcgtgct tcaggcggcg
     3121 gccgctcgag ggacggtggt catgatcgac atggagagcc acgagtacgt cgaccggacc
     3181 ctcgagctcc tccggctgct ccgccggcgc tacgacggcg tcggcgtcgc gctccagtcg
     3241 tacctgtacc gcacggccga cgacatcttc tcgctgccgg agggaacgcc ggtgaggtta
     3301 gtcaagggcg cgtacctcga gccgcccgac gtcgcgttcc cgaagcgcgc cgacgtcgac
     3361 cggagctacg cccgtctgtt cgcgacgctc gcctcgcgcg gccacgaggt gcacgtcgcg
     3421 acgcacgacc cgacgctcct cgccggtgcg gccggcttcg tcgagcgccg aggcatcccg
     3481 tgggagcgga tcgagctcca gatgctctac gggatccgga cggacctcca gcgcgagcac
     3541 gcgcggcgcg ggcatcccgt ccggacgtac atcccgtacg gcgaagagtg gtatccctac
     3601 ctcacgcgcc ggctcgcgga gcggccggcg aacgtgtggt tcttcgcgag ccacgtcctg
     3661 aggaggggca catgaacgag cgactggtcg cgttcctcgg aaccggcaag atgggggagg
     3721 cgctggtctc cgggctcatc cgctcggggg gacggacgtc cgaggagatc atcgtcaccg
     3781 cgcggcggga ggagcgtgcc cgcgagctcg ccgaccgcta cggcctcacc gcgacactgt
     3841 cgaacgccga ggcgatcgag tgggcgccca cgatgatcct gaccgtgaag ccgcaggaca
     3901 tgggggcgct gctcgaccag atcgcggacg tggtcgagcc gaagcacctc gtgatctcgt
     3961 tcgcggcggg gatccgctgc gcctcgatcg aggcccggct ccagcccggc gtcccggtcg
     4021 tgcgcgtgat gtcgaacgtc ccggtgctcg tcgacgaggc gatgtcggcg atcagccccg
     4081 gcgccgtcgc cgaggacaag cacctcgcga tcgcgcagga gctcctcggg tacgtcggaa
     4141 gggtggtccg gctcgcggag cagcaccagg acgcgatcac cgcgacgtcc ggctcggggc
     4201 cggcatactt cgcgctgctc gccgaggcca tgatcgacgc gtgcatcctg ctggggctct
     4261 cgcgcgacgt ggcgaccgag ctcatcgtcc agacgatgct gggttcggcg aagatgttgc
     4321 gcgatacagg gaagcatccc gtggagatcc gcgagatggt gacctcgccg gggggtacga
     4381 ctatcgcggc gatccgccat ctcgagcagg ccggcgtccg cgccgcgttc ctcaatgcga
     4441 tcgacgcagc gaggcagcgc agcgccgagc tcgcgaaggg gtccgaggcc gaggaggtgt
     4501 gatgggcaga ggcgaccgca agaaggtccg ctgggcgcgc gaccgtgagc ggaagaagca
     4561 cgagcgcgag aagcggaagg cggccgagcg ccaggaggcg cggcgcgccg cccgataggg
     4621 gcgcgaggag gtcgagcatg ttcccactgt cgaagggacg ggtcgcgaag caggcgcacg
     4681 tcggcctgcc gggtggcacg tacgaggagg agcacgggcg cgaggccttc gagggccgcg
     4741 cgtcgcacct gtaccgcagc catccgccga cggcgtgggt gaggatcgag gggcacctgc
     4801 gcccgcgcgc gtacgacctc aacccgctga agcccgcgaa cggcgagcgc ggcgactgga
     4861 cgcggatcct gtggaacgac gacgtcgcga tctacgtctc gatgcagccg gtcgcgcgcg
     4921 actggttcct gcgcgactcc gacggcgacc tctgctactt cgtgcaccgc ggcgagggca
     4981 cgctcgagac cgactacggg ccgcttccgt tccgggccgg ggacttcctc gtggtgccca
     5041 aggggacgac gcaccggctc gtccccgaga cgcaggacaa cttcttctac gtcgtcgaag
     5101 ggcgcggcga gtactcggtt cccgagaagg gcatcctcgg gaagcacgcg ctgttcgatc
     5161 ccggcgtgct cgagaccccg gagcccgagc cgcacgacga ggagggcgag ttcgaggtcc
     5221 gcgtcaagcg cgacgaccgg tacacggtgc tgacgtaccc gttccacccg atcgacggcg
     5281 tgggctggca gggcgacctg tgcccggtcc ggctgaacgt cgaggacttc cgcccggtcg
     5341 gctcgccgcg gtaccacctg ccgccggcgg tgcacgtgac gtggaagagc gaggggttcg
     5401 agatc
//
LOCUS       DASZOB010000044         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131709, whole genome shotgun sequence.
ACCESSION   DASZOB010000044 DASZOB010000000
VERSION     DASZOB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131709"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="rimM"
                     /locus_tag="VG709_00705"
     CDS             <1..431
                     /gene="rimM"
                     /locus_tag="VG709_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWC31629.1"
                     /translation="PHGLDGSFVVEDASDDADRFAPGARLYAGGEPATVLTSKRAGGR
                     LVTKLDRAVERGTELAVPRHDLPEPGEGTYYVSDLVGLAVEEEGGRVLGRVQEVAAGV
                     ANDALELDSGVALPLVEDCVRSVDLERRRIVVAQGFADQD"
     gene            442..1098
                     /gene="trmD"
                     /locus_tag="VG709_00710"
     CDS             442..1098
                     /gene="trmD"
                     /locus_tag="VG709_00710"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWC31630.1"
                     /translation="MQIDIFTLLPHAFAWLTEQRPVASVLGSELELRLFNYRDFTPLS
                     GGQVDDEPYGGGPGMVLRVDVVSAALEAVYGESPDRRVIALTPQGRPLDQALVEELAR
                     EEEFALLSARFEGFDERILEHVADEAVSIGPYVVSGGELPAMVVLDAVARRLPGAIRA
                     ESGAVETFSEALDGGVEFPQYTRPAEYRGWRVPDVLLSGNHAEIGRWRSERSRERSRS
                     "
     gene            1095..>1681
                     /gene="lepB"
                     /locus_tag="VG709_00715"
     CDS             1095..>1681
                     /gene="lepB"
                     /locus_tag="VG709_00715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWC31631.1"
                     /translation="MRGPVDRLTASLPRPWATVVDWVVTIVVAVGAVLAIKAWVVNPY
                     RIPSSSMEPTLHCADPQPGCLASFSDRVLANRFIYHFREPERGDIVVFETPPEARARC
                     GAGGTFVKRIIGVPGDRLVQRDGVLRVNRRALEEPYLEQSGQLGQDFRADVPPGYYFM
                     MGDNRTQSCDSREWGPVPRENLIGPVFAVYWPPQRV"
BASE COUNT          221 a          595 c          611 g          254 t
ORIGIN      
        1 ggccgcacgg gctcgacggc tcgttcgtcg tcgaggacgc gagcgacgac gccgaccgct
       61 tcgcacccgg cgcacggctc tacgccggcg gcgagccggc caccgtgctc acgagcaagc
      121 gcgcgggcgg gcgcctcgta acgaagctcg accgcgcggt cgaacgaggc acggagcttg
      181 ccgtcccgcg gcacgatctc cccgagccgg gagaagggac gtactacgtc tccgacctcg
      241 tcgggctcgc cgtcgaggag gagggaggcc gcgtgctcgg gcgcgtgcag gaggtcgccg
      301 ctggcgtcgc gaacgacgcg ctcgagctcg attcgggcgt cgcgcttccg ctcgtcgagg
      361 actgcgtccg ctcggtcgat ctcgaacgac gccgcatcgt ggtggcgcag gggttcgccg
      421 accaggacta agtttgaacg agtgcagatc gacatcttca ccttgctccc gcatgcgttc
      481 gcctggctca cggagcagcg cccggtcgcg tccgtgctcg gctcggaact cgagctgcgg
      541 ctgttcaact accgtgactt cacgccgctc tccggcggcc aggtcgacga cgagccctac
      601 ggcggcgggc ccggcatggt gctgcgcgta gacgtcgtct cggccgcgct cgaggccgtc
      661 tacggcgagt cgcccgaccg gcgcgtaatc gcgctgacgc cgcaggggcg ccctctcgac
      721 caggcgctcg tggaggagct ggcccgcgag gaagagttcg cgctcctctc ggcgcgcttc
      781 gagggcttcg acgagcggat tctcgagcac gtcgccgacg aggcggtctc gatcggcccg
      841 tacgtcgtct ccggcggcga gttgccggcg atggtcgtcc tcgatgccgt ggcgcgccgg
      901 ctcccgggtg ccattcgcgc ggagagcggc gccgtcgaga cgttctccga ggcgctcgac
      961 ggcggcgtcg agttcccgca gtacacgcgc cccgcggaat accgggggtg gcgcgtcccc
     1021 gacgtcctcc tctcgggcaa ccacgcagag atcgggcgct ggcggagcga gcgaagtcgt
     1081 gagcggtcgc ggtcgtgagg gggcccgtcg accggctgac cgcctcgctc ccgcgtccat
     1141 gggcgacggt ggtcgactgg gtcgtgacga tcgtcgtcgc ggtcggggcg gtgctcgcga
     1201 tcaaggcctg ggtcgtgaac ccctaccgga tcccgtcgtc ctcgatggag ccgacgctcc
     1261 actgcgccga cccgcagccg ggctgcctgg cgagcttctc cgaccgggtg ctcgcgaacc
     1321 gcttcatcta ccacttccgc gagcccgagc gcggtgacat cgtcgtcttc gagacgccgc
     1381 ccgaggcgcg cgcccgctgc ggcgcgggcg gaacgttcgt aaagcggatc atcggcgttc
     1441 ccggcgaccg cctcgtgcag cgcgacggcg tcctgagagt caaccgccgg gcgctcgagg
     1501 agccgtacct ggagcagtcc ggccagctgg gccaggactt tcgggcggac gtgccaccgg
     1561 ggtactactt catgatgggc gacaaccgga cgcagtcgtg cgacagccgc gagtggggcc
     1621 cggtgccgcg ggagaacctg atcggccccg tcttcgccgt ctactggcct ccccagcgcg
     1681 t
//
LOCUS       DASZOB010000045         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_81638, whole genome shotgun sequence.
ACCESSION   DASZOB010000045 DASZOB010000000
VERSION     DASZOB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81638"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..929)
                     /gene="pstB"
                     /locus_tag="VG709_00720"
     CDS             complement(78..929)
                     /gene="pstB"
                     /locus_tag="VG709_00720"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWC31632.1"
                     /translation="MESAPVRIPAVSPEAAPEPERARPAREALFSVDGLSVSYGAAPA
                     LANVSLDVYRNFVTAFIGPSGCGKSTFIRCFNRMNDLIPTARVDGRVLYHGYDLYAAD
                     VDPVEVRKRIGMVFQKPNPFPKSIYDNIAFGPRVLSLSGDVGERVERALRRAALWDEV
                     KDRLKASALSLSGGQQQRLCIARCLAVEPDVILMDEPASALDPISTAAIEDLMHELKR
                     DYTIVIVTHNMQQAARVADMTAFFSVEVTADGRRTGVLVEYDLTSKIFTNPSDPRTEA
                     YVTGKVG"
     gene            complement(935..>1659)
                     /gene="pstA"
                     /locus_tag="VG709_00725"
     CDS             complement(935..>1659)
                     /gene="pstA"
                     /locus_tag="VG709_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWC31633.1"
                     /translation="LSLRLLTEPPSATPELAGARPAILATLYMGLLLFAFVVPIGVGT
                     AVYLEEYANRQRWYNRLLEANIQNLAAVPSIVYGILGLAFLVRGVGLGRVLLAGAMIL
                     TLLVLPIVIVASREAIRAVPDSIRQGAYALGATKWQVIWRQVLPAAVPGVATGSILAL
                     ARAIGETAPLLLVGALTYVAVDPTILGPFTALPVQIFQWTARPQEEFKLLAAAGIIVL
                     LALLLTMNGFAIWLRNRYQRRW"
BASE COUNT          257 a          556 c          606 g          240 t
ORIGIN      
        1 tgtcgaggcc gccgagtgcc tgctcctcga ggcccgcgag ctcgtcttgg aagtgggtgc
       61 gcgtcgtctc ggacacgcta gccgaccttg ccggtgacgt acgcctccgt gcgggggtcg
      121 gatgggttcg tgaagatctt cgaggtgagg tcgtactcga cgaggacgcc ggtgcggcgc
      181 ccgtccgccg tcacctcgac gctgaagaac gcggtcatgt cggcgacgcg cgccgcctgc
      241 tgcatgttgt gcgtcacgat cacgatcgtg tagtcgcgct tgagctcgtg catcaggtcc
      301 tcgatcgcgg ccgtcgagat agggtcgagc gccgaggccg gctcgtccat caggatcacg
      361 tcgggctcga cggccaggca gcgcgcgatg cagaggcgct gctgctgtcc gccggagagg
      421 ctgagcgcgc tcgccttcag gcggtccttc acctcgtccc agagcgcggc gcgccggagc
      481 gcgcgctcga cacgctcgcc cacgtctccg ctcagcgaga gcacgcgcgg gccgaaggcg
      541 atgttgtcgt agatcgactt cgggaacggg ttcggcttct ggaacaccat cccgatccgc
      601 ttccggacct cgaccgggtc gacgtccgcg gcgtagaggt cgtacccgtg gtagaggacg
      661 cgcccgtcca cccgcgccgt cgggatgagg tcgttcatgc ggttgaagca gcggatgaac
      721 gtgctcttcc cgcagccgga cggcccgatg aacgcggtca cgaagttccg gtacacgtcc
      781 agcgagacgt tcgcgagcgc gggcgcggcg ccgtaggaga cgctgagccc gtcgaccgag
      841 aagagagcct cccgggcggg gcgcgcgcgc tcgggttccg gcgccgcctc gggagacacc
      901 gccgggatcc tgacgggtgc gctctccatc ggcgtcacca tctcctctgg tagcgattgc
      961 ggagccagat cgcgaagccg ttcatcgtca ggaggagcgc gaggaggacg atgatccccg
     1021 cggccgcgag cagcttgaac tcctcctgcg gcctcgcggt ccactggaaa atctgcaccg
     1081 ggagcgcggt gaacgggccg aggatcgtcg ggtcgacggc gacgtacgtg agcgccccga
     1141 cgaggaggag cggcgccgtc tcgccgatcg cgcgcgcgag ggcgaggatc gatcccgtcg
     1201 ccacgcccgg gacggcggcg gggagcacct gccgccagat gacctgccac ttcgtcgcgc
     1261 cgagggcgta cgcgccctga cggatggagt cagggaccgc gcggattgcc tcccggctcg
     1321 cgacgatcac gatcgggagg acgaggagcg tgaggatcat cgcgccggcg aggagcactc
     1381 gaccgaggcc gacgccgcgc acgaggaagg cgaggccgag gatcccgtag acgatcgacg
     1441 gcaccgccgc caggttctgg atgttcgcct cgagcaggcg gttgtaccag cgctgccggt
     1501 tcgcgtactc ctccaggtag accgcggtgc cgacgccgat cgggacgacg aacgcgaaca
     1561 gcagcaggcc catgtagagc gtcgccagga tcgccggccg cgcgcccgcg agctccggcg
     1621 tcgccgaagg cggctccgtg agcagccgca ggctcaact
//
LOCUS       DASZOB010000046         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_142153, whole genome shotgun sequence.
ACCESSION   DASZOB010000046 DASZOB010000000
VERSION     DASZOB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142153"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VG709_00730"
     CDS             <1..1647
                     /locus_tag="VG709_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HWC31634.1"
                     /translation="VIDGTAVNVTLTRPALEGFSDLTCVEGFAGPFPCDGVDLLSFVP
                     QDQFNGAGVSDIWGWTDPETGDEYVFLGKTNGTAFFRVTDPYNPVYLGELPNPAALEL
                     RWHDMKVYENHAFIVSESEPHGMQVFDLTRLRGVTEAQEWTRDAWYRLNFAAHNVAIN
                     EDTGYAYIVGGNEGIVAPDHCLHGLHMVDISSPKNPAFAGCYAQDGGPGTAARSVGGP
                     AEDVSPAAYVHDTQCVIYEGPDEDYIDREICFNASEDQVTIVDVTNKLLPQTLGVVVY
                     ENVAYAHQGWLSEDQRFLFVNDELDEQAGNEGRKEGDSLTNTRTIVLDVSDLENPKVH
                     FEHLHETVSIDHNNYVHEGLLYQSNYTSGLRVLETAFVDDEENPRLEQVAFFDTYPET
                     DDPIFEGTWSNYPFFESGTIAVSGIGEGLFLLRLSDGGTGAAAVDIACADCPIEVRAG
                     GRGGTAVVVSNVGDVDDVYGLAITQQPEGWSADVRPAELDVEAGGQRRARVNVGVPRG
                     AEPGTYTVVVTATSTVDPAVSASAELPVLVDEGKGQAKRR"
     gene            complement(1692..2189)
                     /locus_tag="VG709_00735"
     CDS             complement(1692..2189)
                     /locus_tag="VG709_00735"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HWC31635.1"
                     /translation="MIEGDRSPRAYVAVCGPADATEEERALAEEVGRRLAEADAVVLC
                     GGLDGVMDAVARGTAKGGGISIGLLPGEDRADAGTNLTLSLPTGMGEIRNALLVRAAD
                     VVIAISGEFGTLSEIAFALRTQKPVVGLDTWELAKAGEPVDAFVRARTPEEAVERSLE
                     LARGS"
     gene            complement(2186..>2401)
                     /locus_tag="VG709_00740"
     CDS             complement(2186..>2401)
                     /locus_tag="VG709_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HWC31636.1"
                     /translation="IFIYDGYPGGAGITERGFASGERLLRATLEAIRVCPCQAGCPSC
                     VQSPKCGNGNEPLDKHRAADLLREMLR"
BASE COUNT          377 a          863 c          812 g          349 t
ORIGIN      
        1 gtcatcgacg gcacggcggt caacgtgacg ttgacgcggc cggcgctcga ggggttctcc
       61 gacctgacct gcgtcgaggg gttcgccggt ccgttcccgt gcgacggcgt cgacctgctg
      121 tcgttcgtgc cccaggacca gttcaacggc gcgggcgtca gcgacatctg gggctggacc
      181 gatccggaga ccggcgacga gtacgtcttc ctcggcaaga ccaacgggac ggcgttcttc
      241 cgggtcaccg acccctacaa ccccgtgtac ctcggcgagc tcccgaaccc cgccgccttg
      301 gaactgcgct ggcacgacat gaaggtgtac gagaaccacg cgttcatcgt gagcgagtcc
      361 gagccgcacg ggatgcaggt cttcgacctc acccgtctgc gcggcgtgac cgaggctcag
      421 gagtggaccc gtgacgcctg gtaccgactc aacttcgcgg cgcacaacgt cgcgatcaac
      481 gaggacaccg ggtacgcgta catcgtcggt ggcaacgagg ggatcgtggc tcccgaccat
      541 tgcttgcacg ggctccacat ggtcgacata tcctcgccga agaacccggc gttcgcgggc
      601 tgctacgcgc aggacggcgg gccggggacg gccgcccgca gcgtgggagg ccccgccgag
      661 gacgtctccc cggccgcgta cgtccacgac acgcagtgcg tgatctacga gggccccgac
      721 gaggactaca tcgatcgcga gatctgcttc aacgcctcgg aggaccaggt cacgatcgtc
      781 gacgtcacga acaagctgct cccccagacc ctgggcgtcg tcgtgtacga gaacgtcgcg
      841 tacgcccacc agggctggct ctcggaggac cagcgcttcc tgttcgtgaa cgacgagctc
      901 gacgagcagg ccggcaacga gggcaggaaa gagggtgact cgctcacgaa cacccgcacg
      961 atcgtcctcg acgtctccga cctcgagaac ccgaaggtcc acttcgagca cctccacgag
     1021 acggtctcga tcgaccacaa caactacgtg cacgaggggc tcctctacca gtcgaactac
     1081 acgagcgggc ttcgggttct agagaccgcg ttcgtggacg acgaggagaa cccgcgactc
     1141 gagcaggtgg cgttcttcga cacctacccg gagaccgacg acccgatctt cgaggggacc
     1201 tggtcgaact acccgttctt cgagtccggc acgatcgccg tcagcggcat cggcgagggg
     1261 ctgttcctcc tcaggctcag cgacgggggg accggcgccg ccgccgtcga catcgcctgc
     1321 gccgactgcc cgatcgaggt gcgcgccggc ggccgcggcg gcaccgccgt ggtcgtgagc
     1381 aacgttggcg acgtggacga cgtctacggg ctcgccatca cgcagcagcc cgaggggtgg
     1441 tcggccgacg tccgtcccgc ggagctcgac gtcgaggctg gcgggcagcg ccgtgcccgg
     1501 gtgaacgtcg gagtgccgcg aggcgccgag cccggcacct acacggtggt cgtgaccgcg
     1561 acgtcgacgg tcgatccggc cgtctccgcg tccgcggagc tccctgtcct cgtggacgag
     1621 gggaagggcc aggccaagcg ccgctgagga cggccggtcg agcgggtccg gggttcgagg
     1681 tcggggctcc gttaggagcc ccgcgcgagc tcgaggctcc gctcgacggc ctcctcgggc
     1741 gtccgcgccc ggacgaacgc gtcgaccggc tcgccggcct tcgccagctc ccaggtgtcg
     1801 aggccgacga cgggtttctg cgtccgcagc gcgaacgcga tctccgacag cgtcccgaac
     1861 tcgcccgaga tagcgatcac gacgtcggcc gcgcgcacga gcagcgcgtt gcggatctca
     1921 cccatcccgg tggggagcga cagcgtcagg ttcgtgccgg cgtctgcgcg gtcctcgccc
     1981 gggagcagcc cgatcgagat ccctcctccc ttcgcggtgc cgcgggcgac ggcgtccatc
     2041 acgccgtcga gcccgccgca gagcaccacg gcgtccgcct ccgcgagccg gcgacccacc
     2101 tcctcggcga gcgcgcgctc ctcctcggtc gcgtctgccg ggccgcagac ggcgacgtag
     2161 gcccgcggcg agcggtctcc ctcgatcacc tcagcatctc cctgagcagg tcggcggcac
     2221 ggtgcttgtc gagcggctcg tttccgttcc cacatttcgg cgactgcacg catgacgggc
     2281 accccgcctg gcacggacag acccggatcg cctcgagcgt cgcgcgcagg agccgctctc
     2341 cgctcgcgaa cccccgctct gtgatgcccg cgccgccggg gtagccgtcg tagatgaaga
     2401 t
//
LOCUS       DASZOB010000047         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_102063, whole genome shotgun sequence.
ACCESSION   DASZOB010000047 DASZOB010000000
VERSION     DASZOB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102063"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VG709_00745"
     CDS             <1..603
                     /locus_tag="VG709_00745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HWC31637.1"
                     /translation="EIVGFDIHVNTSYRLTTNGSAFDLETIVRHEAGHAIGLGHTDRY
                     RNVMYPVIGWSQTKPLGPGDRAGNRALHPYRDYGVRVMERDPDGQMRMLPGGRSTVAV
                     KLKNTGMTPWNGRFRGIQLRTDGPRKRDSRFFDPPTWVSPSQPGERITGVVCHGEVVT
                     VRWAMKAPSEMKRYREAFGVRIGKTWMTNESPVWDVAVPG"
     gene            714..1820
                     /locus_tag="VG709_00750"
     CDS             714..1820
                     /locus_tag="VG709_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWC31638.1"
                     /translation="MTTGRLAAFVLAAALSSAGCASGGPSIFADLREQPTPSAAAAPA
                     AIVVRGEPIVRVVDRVAPAVVNVRTDVPENVGRNDRVPEGTGFIVDESGVVVTNFHVI
                     EQATVNIRVTTAEGESFDATVIGSDADADIAVLTVEADDALPTVELGDSENARLGQRV
                     VALGFALGLEGGPSVTSGILSAKDRSIEARDPAVGVRQYEDLLQTDAAINPGNSGGPL
                     VDMSGRVIGINTAGVGAASAENIGFAIAIDRASPLIERAIENPAAPLAYLGVQTRDVD
                     PVVQAQLGLASDSGALILEVVEGEPAERAGLTAGDIIVSFAGDPVDGSESLQEEILSH
                     SPGDAVEVGVVRPDGDTETVEVTLGERLVPPIEG"
     gene            1817..>2187
                     /locus_tag="VG709_00755"
     CDS             1817..>2187
                     /locus_tag="VG709_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011075538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31639.1"
                     /translation="MSPEAFRAGDRAMLRDGRGRTFLIRLEPGATFHYHGGAVPHDLV
                     IGSDEGTTVHSTTGTALTCFRPRLADFVLKMGRGAQVIYPKDLGAILVYADIFPGARV
                     LEAGTGSGALTLALCRAVGPDG"
BASE COUNT          327 a          747 c          803 g          310 t
ORIGIN      
        1 gagatcgtcg ggttcgacat ccacgtcaac acgtcgtacc gactgaccac gaacgggagt
       61 gcattcgatc tcgagacgat cgtccgccac gaggccggcc atgcgatcgg cctgggacac
      121 accgaccggt atcggaacgt catgtacccg gtaatcggtt ggagccagac gaagccgctc
      181 ggtccgggcg accgggccgg caaccgcgcg ctgcatccgt accgcgacta cggcgtccgg
      241 gtgatggagc gggatcccga cggacagatg cggatgctgc cgggtggccg ctccacggtg
      301 gccgtcaagc tgaagaacac cgggatgacg ccgtggaacg gccggttccg cggcatccag
      361 ctccgcaccg acgggccgcg caagcgcgac agccgcttct tcgacccccc gacctgggtg
      421 tcgccgagcc agccgggcga gcgcatcacc ggcgtcgtgt gccacggcga ggtcgtcacg
      481 gtgcgctggg cgatgaaggc gccgtcggag atgaagcgtt accgcgaggc gttcggcgtc
      541 cggatcggca agacgtggat gacgaacgag tcgcccgtat gggacgtggc ggtgcccggc
      601 tagggcccgc ccgtatgagc gacggggccg cgcagggcgg tgcgcggtcc cgtcgttctc
      661 atgtcccggc atcctggggg gtccggaatt ccgccgatgc tatcctcgcc cgcgtgacca
      721 ccggacgcct cgcggctttc gtgctggccg ccgccctctc ttcggctgga tgtgcgagcg
      781 gcggaccgtc gatcttcgcg gatctccggg agcagccgac gccgtcggcg gcggccgccc
      841 ccgcggcgat cgtcgtccgc ggcgagccga tcgtgcgcgt cgtcgaccgt gtggctccgg
      901 cggtcgtgaa cgtccgaacc gacgttcccg agaacgtcgg tcggaacgac cgggtcccgg
      961 agggaacggg gttcatcgtc gacgagagcg gcgtggtcgt gacgaacttc cacgtgatcg
     1021 agcaggccac ggtcaacatc cgggtcacga ccgcggaggg ggagtcgttc gacgccaccg
     1081 tgatcggctc cgacgccgac gccgacatcg cggtcctcac cgtggaggcc gacgacgcat
     1141 tgccgacagt cgagctcggc gattcggaga acgcccgact agggcagcgg gtcgtcgcgc
     1201 tcggcttcgc gctcggcctc gagggagggc cgtccgtcac gtccgggatc ctgtcggcga
     1261 aggaccgttc gatcgaggct cgcgaccccg cggtgggcgt ccgtcagtac gaggatctcc
     1321 tgcagaccga cgcggcgatc aacccgggga actccggcgg cccgctcgtc gacatgtccg
     1381 gacgcgtgat cgggatcaac acggcggggg tcggcgcggc atccgcggag aacatcggct
     1441 tcgcgatcgc gatagaccgc gcgagcccgc tgatcgagag ggccatcgag aaccccgcgg
     1501 cgccgctcgc atacctgggc gtccagacgc gggacgtcga tccggtcgtg caggcgcagc
     1561 tcgggctcgc gtccgacagc ggcgcgttga tcctcgaggt cgtcgaggga gagccggcag
     1621 agcgcgcggg gctcacggcc ggcgacatca tcgtttcgtt cgccggtgac ccggtcgacg
     1681 ggagcgagag ccttcaggag gagatcctca gccactcgcc gggcgacgcc gtggaggtgg
     1741 gcgtcgtgcg gccggacggc gacaccgaga cggtcgaggt gaccctcgga gagcgcctgg
     1801 tgccgcccat cgagggatga gccccgaggc gttccgcgcc ggcgaccgcg cgatgctgcg
     1861 cgacggccgc ggccgcacgt tcttgatccg gctcgagccc ggggcgacgt tccactacca
     1921 cggcggcgcg gtgccgcacg acctcgtgat cggcagcgac gagggaacga cggtccactc
     1981 gacgaccggg acggcgctca cgtgcttccg cccacgcctc gccgacttcg tgctgaagat
     2041 gggccgcggc gctcaggtga tctacccgaa ggacctcggc gcgatcctcg tctacgccga
     2101 catcttcccg ggtgcgcgcg tcctcgaagc cgggacgggg tcgggagcgc tgacgctcgc
     2161 cttgtgccgc gccgtgggac ccgacgg
//
LOCUS       DASZOB010000048         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_81718, whole genome shotgun sequence.
ACCESSION   DASZOB010000048 DASZOB010000000
VERSION     DASZOB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81718"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VG709_00760"
     CDS             <1..954
                     /locus_tag="VG709_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC31640.1"
                     /translation="PRPADARGAGVTDVAAPERTVEPTGGATAAGAPIRQASLWRDAW
                     RRYVRNRGAVIAGVIFLAIVLYALVAPVLSPHDPYEVDFSKAYQTPNSTHLLGTDQFG
                     RDLFTRTALGGRVSIGIGFAATIVILIMGILYGGISGFVGGKLDNALMRFLDALYGLP
                     FLPFAIITLAIVGTVNFMTMVIALSIVSWFTAARVVRGQIITLKENDYVRAAQAVGAR
                     WYRVFFRHLLPNTLGVLTIFMFLELPGVVLGEAFISFIGLGINPPEASWGSMAQDGRD
                     AYRAHPHVIIIPSVAIATLVLCANFIADGLRDALDPRTRET"
     gene            958..>1896
                     /locus_tag="VG709_00765"
     CDS             958..>1896
                     /locus_tag="VG709_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC31641.1"
                     /translation="MALLEVDDLRTHFFTREGTVRAVDGVSFSVDMRKTLGIVGESGC
                     GKSVTALSIMGLIPKPPAKIVSGSVMFEGRDLTTLSERELEDVRGRKIAMIFQDPMTS
                     LNPTLKIGTQITETLRRHLDLSKDAARKRAVELLEEVRIPKAAERVNDYPHRFSGGMR
                     QRVMIAIALACNPALLIADEPTTALVVTIQAQILDLLDDLRREHDMGMIMITHDMGVI
                     AETADDVAVMYAGQIVEQAPVVNLFECPAHPYTEALLGALPNLEAEGVRDGRLTAIPG
                     LPPDLVNPPPACRFAPRCPYADSGDSCTREPPELREV"
BASE COUNT          288 a          677 c          633 g          298 t
ORIGIN      
        1 cctcgacccg cggacgcgcg aggtgcgggc gtgacggacg ttgcagcgcc ggaacgcacg
       61 gtggagccca ccggcggtgc gaccgcggcg ggcgccccga tccgccaggc gagtctctgg
      121 cgcgacgcct ggcggcgcta cgtccggaac cgtggagcag tcatcgccgg ggtcatcttc
      181 ctcgcgatcg tcctgtacgc gctcgtcgcg ccggtgctct cgccgcatga cccgtacgag
      241 gtggacttct cgaaggcgta ccagacgccg aactcgaccc acctgctcgg aaccgaccag
      301 ttcggacgcg acctcttcac acggaccgcg ctcggcggcc gcgtgtcgat cgggatcggg
      361 ttcgcggcca cgatcgtgat cctgatcatg ggaatcctgt acggcggcat ctcggggttc
      421 gtcggcggaa agctcgacaa cgcgctcatg cgcttcctcg acgcgctcta cggcctgccg
      481 ttcctgccct tcgcgatcat cacgctcgcc atcgtcggca cggtgaactt catgacgatg
      541 gtgatagcgc tctcgatcgt ctcgtggttc accgcggcgc gcgtcgtgcg cggccagatc
      601 atcacgctca aggagaacga ctacgtccgg gcggcgcagg cggtcggagc gcgctggtac
      661 cgcgtcttct tccgccacct cctgccgaac acgctcggcg tcctcaccat cttcatgttc
      721 ctggagctcc cgggcgtggt cctcggcgag gcgttcatct ccttcatcgg cctcggcatc
      781 aacccgcccg aagcgtcgtg gggctcgatg gcgcaggacg ggcgggacgc gtaccgggcg
      841 cacccgcacg tcatcatcat cccgagcgtc gcgatcgcga cgctcgtcct ctgcgcgaac
      901 ttcatcgcgg acgggctccg cgacgcgctc gacccgcgga cgagggagac gtagggcgtg
      961 gcgctcctcg aggtcgacga cctccggact catttcttca cgcgcgaggg caccgtgcgc
     1021 gcggtcgacg gggtgagctt ctccgtcgac atgaggaaga cactcgggat cgtcggggag
     1081 tcgggctgcg ggaagtcggt gacggcgctc tcgatcatgg ggctcatccc gaagccgccg
     1141 gcgaagatcg tgagcggctc cgtcatgttc gagggccgcg acctgacgac gctctcggag
     1201 cgcgagctcg aggacgtccg cgggcggaag atcgcgatga tcttccagga cccgatgacg
     1261 tcgctgaacc cgacgctgaa gatcgggacg cagatcaccg agacgctccg ccgccacctc
     1321 gacctctcga aggacgcggc gaggaagcgc gccgtcgagc ttctcgagga ggtgcgaatc
     1381 ccgaaggcgg ccgagcgcgt gaacgactat ccgcaccgct tctcgggcgg gatgcgccag
     1441 cgcgtcatga tcgcgatcgc gctcgcctgc aacccggcgc tcctgatcgc cgacgagccg
     1501 accacggcgc tcgtcgtcac gatccaggcc cagatcctcg acctgctcga cgacctgcgc
     1561 cgcgagcacg acatggggat gatcatgatc acgcacgaca tgggcgtgat cgccgagacg
     1621 gccgacgacg tggcggtcat gtatgccggc cagatcgtcg agcaggcgcc cgtggtcaac
     1681 ctcttcgagt gtcccgcgca cccctacacc gaggcgctgc tcggagcgct gcccaacctc
     1741 gaggccgaag gcgtccgcga cggccggctg accgcgattc cggggctccc gcccgacctc
     1801 gtcaatccgc cccccgcctg ccggttcgcg ccgaggtgtc cctacgccga ctcgggcgac
     1861 tcgtgcacgc gcgagccgcc ggagctccgc gaggtc
//
LOCUS       DASZOB010000049         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51402, whole genome shotgun sequence.
ACCESSION   DASZOB010000049 DASZOB010000000
VERSION     DASZOB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51402"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VG709_00770"
     CDS             complement(<1..1042)
                     /locus_tag="VG709_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWC31642.1"
                     /translation="MTETPPEAVRRRDWGPRRRRYALPLSLIALGLAYPWWYDLPGIS
                     ALTLDVLGMKLDIAFVIAVYVLLALGLNIVVGYAGLLDLGYVAFYALGAYTVGWFASD
                     HFSGHNFHFGSTAVETLPGIHLNFWLVIILGGCIAAIAGVIIGWPTLRLRGDYLAIVT
                     LGFGEIIPDVFRNADALPIPTGFAATPPFFGVDTVNFTDGVRGIRQLDRPGFGDSWSE
                     ATGGLFPDRFTSIDLAPWFLTVVVLALLVVYGNFRLRDSKMGRAWVAVREDEVAASAM
                     GVPLMRTKLWAYGIGAVFGGFAGAVYGSFIGSIFPTSFSFNISILVLCMVIVGGMGNI
                     YGGVLGAVTLEYL"
     gene            complement(1039..>1994)
                     /locus_tag="VG709_00775"
     CDS             complement(1039..>1994)
                     /locus_tag="VG709_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWC31643.1"
                     /translation="NLLDGLSNGSIWALVAIGYTLVYGIIELINFAHGDNFMLGTFET
                     KTIVQDGQFQVPLYPLTWELFIPLGTATVAASSGWHRAYAIAMAVFFSMLLCMVINVS
                     IERIAYKPLRNQPRLAPLITAIGFSFLLQNVGLVWKDAGPQPVPDILPEGDIFAFSGI
                     TYSWKDFIVLAITAPLLIALSWLVAATKQGKAMRATAQDRDASAMMGINVDRTISFTF
                     ALGGALAGAAGAIYALYIGTTKFDLGFRLGLFAFTAAVLGGIGNLTGAVLGGVLLGMI
                     AAYNEGFADARWTTTIIFSILVLILVFRPSGLLGARPPEGQ"
BASE COUNT          379 a          641 c          714 g          260 t
ORIGIN      
        1 tcaggtactc gagcgtcacc gcccccagca cgcccccgta gatgttgccc attccgccaa
       61 caatgaccat gcagaggacc aggatcgaga tgttgaacga gaagctcgtc gggaagatcg
      121 acccgatgaa cgagccgtag acggcgcctg cgaagccgcc gaagacggcg ccgataccgt
      181 acgcccacag cttcgtccgc atcagcggca cgcccatcgc cgacgctgcc acctcgtcct
      241 cgcgcacggc gacccacgcc cgccccatct tcgagtcgcg cagccggaag ttgccgtaga
      301 cgacgagcag cgccagcacg acgaccgtca gaaaccacgg cgccaggtcg atactcgtga
      361 accgatcggg gaagaggccg ccagtcgcct ccgaccacga gtcgccgaaa ccgggccggt
      421 cgagctgccg aatgccgcgg acgccgtcgg tgaagttgac ggtgtcgacg ccgaagaacg
      481 ggggcgtcgc cgcgaagccg gtcgggatcg gcagcgcgtc cgcattgcgg aagacgtccg
      541 gaatgatctc gccgaagccg agcgtgacga tggcgaggta gtcgccccgc aggcgcagcg
      601 tcggccagcc gatgatcacg cctgcgatcg ccgcgatgca gccgccgagg atgatcacga
      661 gccagaagtt caggtggatg ccgggcagcg tttcgacggc ggtcgagccg aagtggaagt
      721 tgtgcccgga gaagtggtcg gaggcgaacc agccgacggt gtacgcgccg agtgcataga
      781 acgcgacgta gccgagatcg agcagcccgg cgtacccgac gacgatgttg aggccgagcg
      841 cgagcaggac gtagaccgcg atcacgaacg cgatgtcgag cttcatcccg agcacgtcga
      901 gcgtcagcgc gctgatcccg gggaggtcgt accaccacgg gtaggcgagg ccgagcgcga
      961 tcagtgagag cgggagggcg tagcggcggc ggcgcggccc ccagtcgcgc ctccgcacgg
     1021 cctcgggcgg ggtctccgtc actgcccctc cggcggccgc gcgccgagga gcccggacgg
     1081 gcggaagacg aggatgagca cgaggatcga gaagatgatc gtcgtcgtcc agcgcgcgtc
     1141 ggcaaacccc tcgttgtacg cggcgatcat ccccaggagc acgccgccga gcacggcgcc
     1201 ggtgaggttc ccgatgccgc cgagcaccgc ggcggtgaag gcgaagaggc cgaggcggaa
     1261 gccgaggtcg aacttcgtcg tgccgatgta gagcgcgtag atggcgccgg ccgcgcccgc
     1321 gagcgcgccg ccgagggcga acgtgaagga gatcgtccgg tcgacgttga tgcccatcat
     1381 cgcggaggcg tcgcgatcct gggcggtcgc ccgcatcgcc ttcccctgct tcgtcgcggc
     1441 gaccagccac gagagcgcga tcaggagggg agccgtgatc gcgaggacga tgaagtcctt
     1501 ccacgagtac gtgatcccgc tgaaggcgaa gatgtcgccc tccgggagga tgtcggggac
     1561 gggctgcggc ccggcgtcct tccagacgag gccgacgttc tgcagcagga acgagaagcc
     1621 gatcgccgtg atgagcggcg cgagccgcgg ctggttgcgc agcggcttgt acgcgatccg
     1681 ctcgatcgag acgttgatca ccatgcagag gagcatcgag aagaagaccg ccatcgcgat
     1741 cgcgtacgcg cggtgccagc cggacgaggc ggcgaccgtg gccgtcccga gcgggatgaa
     1801 gagctcccag gtgagcggat agagcgggac ctggaactgg ccgtcctgga cgatcgtctt
     1861 cgtctcgaac gtgccgagca tgaagttgtc cccgtgcgcg aagttgatca gctcgatgat
     1921 cccgtagacg agcgtgtagc cgatcgccac gagcgcccag atcgacccgt tggacaggcc
     1981 gtcgaggagg ttgt
//
LOCUS       DASZOB010000050         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_41023, whole genome shotgun sequence.
ACCESSION   DASZOB010000050 DASZOB010000000
VERSION     DASZOB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41023"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VG709_00780"
     CDS             complement(<1..375)
                     /locus_tag="VG709_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC31644.1"
                     /translation="MVVRHPLFTDEHDDLRRSVRAFVESELAPHALDWEREEFPDWVF
                     KRMGDLGLLGLRYPGEHGGQGGDWGHTIVMAEEIARIGSGGVGMAIAVQTDMATPPIL
                     RFGTEEQKQRYLVPSIRGEKISC"
     gene            450..875
                     /locus_tag="VG709_00785"
     CDS             450..875
                     /locus_tag="VG709_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWC31645.1"
                     /translation="MAQQQQEVTEIIGGISLFADLSRPQLEGVAHTFEEEWYPDGQRI
                     LRQGFQGNNFYVILDGEAVVRIDGQDRVKLARGDFFGEVSVLLGEAPTADVVAARPLR
                     CLVMPGSQVQGFLTAHPQVMFRLLQAQARRLRATLQWQS"
     gene            863..1252
                     /locus_tag="VG709_00790"
     CDS             863..1252
                     /locus_tag="VG709_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31646.1"
                     /translation="MAELGRYPPGDYQVVVVGSGPGGTQISYFLDRLGVRHAFLSADE
                     EGAGMFRKWPFFQRLISWTKPYAPAERGSRWYEWFDWNSLLAEEPEHRALQPQFMDGT
                     SEFPSRMEMQQQLEAFIERTETIPRYG"
     gene            1274..2347
                     /locus_tag="VG709_00795"
                     /pseudo
     CDS             1274..2347
                     /locus_tag="VG709_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
BASE COUNT          364 a          824 c          808 g          351 t
ORIGIN      
        1 gcaggagatc ttctcgccgc ggatcgaggg gacgaggtag cgctgcttct gctcctcggt
       61 cccgaaccgc aggatcggcg gcgtcgccat gtcggtctgc acggcgatcg ccatcccgac
      121 gccgcccgag ccgatacgcg cgatctcctc cgccatcacg atcgtgtgac cccagtcgcc
      181 gccctgcccg ccgtgctcgc ccgggtaccg caaccccagc aacccgagat cgcccatccg
      241 cttgaacacc cagtcgggaa actcctcgcg ctcccagtcg agcgcgtgcg gggcgagctc
      301 ggactcgacg aacgcgcgca ccgagcgccg gaggtcgtcg tgctcgtcgg tgaacagcgg
      361 atggcgcacc accatcggaa gtttacgccc gggtaggttc ggcgggaacg cctcgacggg
      421 cccggcggga cccgactaag gtgccgccga tggcacagca acagcaggag gtcaccgaga
      481 tcatcggggg gatctctctg ttcgccgacc tctcgcgtcc gcagctcgag ggcgtcgctc
      541 acacgttcga ggaggagtgg taccccgacg ggcagcgcat tctccggcag gggtttcagg
      601 gcaacaactt ctacgtgatc ctcgacggcg aggccgtcgt gcgcatcgac ggacaggacc
      661 gcgtgaagct cgcgcgcggc gacttcttcg gcgaggtgtc ggtgctcctc ggcgaggcgc
      721 cgaccgcgga cgtcgtcgcg gcgcggccgc tgcgctgcct cgtgatgccg ggctcgcagg
      781 ttcagggctt cctcaccgca cacccgcagg tgatgttccg tctgctccag gcccaggccc
      841 gccggctccg cgccacgctg cagtggcaga gctagggcgg tacccgcccg gcgactacca
      901 ggtcgtcgtc gtcggcagcg gccccggcgg cacgcagatc agctacttcc tcgaccggct
      961 cggcgtccgc cacgcgttcc tctcggccga cgaggagggc gccggcatgt tccggaagtg
     1021 gccgttcttc cagcggctca tcagctggac gaagccgtac gcgccggccg agcgcggcag
     1081 tcgctggtac gagtggttcg actggaacag cctcctggcc gaggagccgg agcaccgcgc
     1141 gctccaaccg cagttcatgg acggcacgtc ggagttcccc tcgcgcatgg agatgcagca
     1201 acagctcgag gctttcatcg agcgcacgga gacgatccct cggtacggct gacggtggga
     1261 gcacgtcgac ctcgaagacg aacgcttcgt cgtcacgacg tccgacgggg tgtaccgctg
     1321 cgacgtgctg atcgtcgcga ccggcatgac gcagccgtgg aagccccagg tcccgggcat
     1381 cgaggacgtc gcgcactacg cggacatggc ggaaccgaag acgtacgccg gcaagcgcgt
     1441 cttcgtgatc gggaagcgga actcggggtt cgaggtcgcc gacggcctgc tgccgtgggc
     1501 gcggtcgatc gtgctcggct ctccccggcc ggcacggctc tcgatcttcg agcacacgat
     1561 cgccgcggcc cgcgctcgct acatgcagcc gtacgaggac cacatcttcg gcggcggcgt
     1621 gtacgtgctg gactgctcca tcgagcgcgt cgagcggacg agcgacgggt accgcgtcca
     1681 cgcgaccggc acgacgaggc ccggcgcgac gacgttcgac gtcgacgagg tgatcgccgc
     1741 gactgggttc ggcacgccgc tcgggaacct gcgcgacctc ggcgtgcgga ccttctcgca
     1801 ggaccgtttg ccgtctcaga cgccgtactg ggagagctcg gacgtgcccg ggatctactt
     1861 cgcgggctcg atcacgcagg ggtcggtcgg gctcaagaag tacgggaagc ctggcgcctc
     1921 ggcatcggtc cacgggttcc ggtacaacgc acgcgtgctc gctgagcacc tcgccgagac
     1981 gcgcttcggc atcgcgatcc cccgccccga gatccggccc gacgacgtcg tgccgttcct
     2041 gctcgccgag gcgtcgcggg gcccggaggt ctggaaccaa cagtcgtacc tcgcgcgagc
     2101 gctgtcgctc actgacggca cggtccgaga catggggatc gtgccgctcg cggagttcgt
     2161 cgacatgaac cgaggcaacg ccgtcgcggt cacggtcgag aacgacgacc gcggtgacat
     2221 ccatcccgcg gtatacctgt gtacgcgcgg gcgcgccgac gagcacctgc tcgacggcga
     2281 cccgatgcac gacttcgaga cggacgagca ccgggtgcag ctcgcgtccg cgctcgaggg
     2341 agcgctt
//
LOCUS       DASZOB010000051         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_162576, whole genome shotgun sequence.
ACCESSION   DASZOB010000051 DASZOB010000000
VERSION     DASZOB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162576"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VG709_00800"
     CDS             complement(<1..324)
                     /locus_tag="VG709_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWC31647.1"
                     /translation="MSRPLIAIGGRRLRAGKVAGWPGAAVAAPDAYADGIRRAGGRPA
                     VVLPEPLDDAEARRLLERFDAVVLMGGGDIDPARYDEPRRERIYGVDAERDAFEIAVA
                     RAAAET"
     gene            complement(321..1283)
                     /locus_tag="VG709_00805"
     CDS             complement(321..1283)
                     /locus_tag="VG709_00805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWC31648.1"
                     /translation="MTREIADDAIALLRDRFDVEVRDEGRPIGRRELLAAVRGRSALV
                     SMLTDRIDDEVLEAAGEELRIVANHAVGIDNVDVEACTRRRVLVTNTLDVLTEATADL
                     TWGLILAAVRRIVEGDRLVRGGRAWSWAPLFMLGRQLGGRTLGIVGLGRIGRAVAARA
                     RGFGMRVLYHSRTRQPDAGPELGAEHRSLDDLLREADVVTLHVPLHEATRRLFGEREF
                     RSMKPTAVFVNTSRGAVVDEAALADALARGEIFAAGLDVYEREPEVHPRLLELDNVVL
                     APHLGSATVDTRVAMGVAVAQNVVAALEGNRPPNLVNDSAWETA"
     gene            complement(1308..>2043)
                     /locus_tag="VG709_00810"
     CDS             complement(1308..>2043)
                     /locus_tag="VG709_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWC31649.1"
                     /translation="NDGGDPGAAWRILQVAVVKRADSTDPDRSGTRVRDLPPPRENPS
                     VIGPLFRWLFQWPYRVVLYVLYRIGVRPWQLTMLSLIANIVCGVLLLTGRRFLPGLLL
                     LPAGLFDIFDGGVARLRGEESRKGALLDSVIDRASDGIVFGCLFYAEFMIHGQMWTAV
                     AALAAMVVSLLVSGVRAEGEAAGLEMSEGSVQRLERYVGLMFGLTAPGMLLPVLVVLT
                     VLSLLTTSQRFMRAWRALPAGGTSHG"
BASE COUNT          309 a          760 c          710 g          264 t
ORIGIN      
        1 cgtctcggcc gcggcgcgcg ccaccgcgat ctcgaacgcg tctcgctccg cgtcgacacc
       61 gtagatgcgc tcacggcgcg gctcgtcgta gcgcgcgggg tcgatgtcgc cgcctcccat
      121 gagcacgacg gcgtcgaacc gctcgaggag cctgcgcgcc tcggcgtcgt cgagcggttc
      181 cgggaggacg accgcggggc gccccccggc gcgccgtatc ccgtcggcgt acgcgtcggg
      241 cgccgcgacg gccgcgcccg gccacccggc caccttcccg gcacgaagcc ggcgaccgcc
      301 gatcgcgatg agcggccggc tcacgccgtc tcccatgcgc tgtcgttgac gaggttcggc
      361 gggcggttcc cctccagcgc ggcgacgacg ttctgggcga cggccacgcc catcgcgacg
      421 cgcgtgtcga ccgtcgcgct cccgaggtgt ggggccagca cgacgttgtc gagctcgagc
      481 agtctcgggt ggacttcggg ctcgcgctcg tacacgtcga gtccggccgc gaagatctcg
      541 ccgcgcgcga gcgcgtccgc gagcgccgcc tcgtcgacga cggcacccct tgaagtgttg
      601 acgaataccg ccgttggctt catcgaccgg aactcccttt cgccgaacag tcggcgcgtc
      661 gcctcgtgca gcggtacgtg gagcgtcacg acgtccgctt cccggaggag gtcgtcgagc
      721 gatcggtgct ccgctccgag ctccggtccg gcatccggtt gccgcgtccg cgaatggtag
      781 agcacccgca tcccgaaccc ccgcgcccgg gcggcgacgg cccgaccgat gcgtccgaga
      841 cccacgatgc cgagcgtcct cccgccgagc tgacgaccca gcatgaacag cggcgcccac
      901 gaccacgcgc ggcccccgcg caccaagcga tctccctcca cgatccggcg cacggccgcc
      961 aggatcagcc cccacgttag gtcggcggtg gcctcggtga gcacgtcgag ggtgttcgtc
     1021 acgagcacgc ggcgccgcgt gcaggcctcg acgtcgacgt tgtcgatgcc gaccgcgtgg
     1081 ttcgcgacga tccggagctc ctcgcccgcc gcctcgagca cctcgtcgtc gatccggtcc
     1141 gtcagcatcg agacgagcgc cgagcggccg cggacggccg ccagcagctc acgccgcccg
     1201 atcggccggc cctcgtcgcg aacctcgacg tcgaaccggt cgcgcagcaa cgcgatcgcg
     1261 tcgtcggcga tctcgcgcgt cacgaggacc ggagggagag acgacaccta gccgtgcgag
     1321 gtcccgccgg ccggcagcgc gcgccacgcg cgcatgaagc gctgcgaggt cgtgaggaga
     1381 ctgagcacgg tcaggacgac gaggaccggg aggagcatcc ccggcgcggt gagaccgaac
     1441 atcagcccga cgtagcgctc gagccgctgg acgcttccct cggacatctc gagccccgcc
     1501 gcctcgccct cggcgcggac gccgctcacg aggagcgaga cgaccatcgc cgcgagcgcc
     1561 gcgacggccg tccacatctg gccgtggatc atgaactccg cgtaaaacag gcacccgaac
     1621 acgatcccgt ccgaggcgcg atcgatcacc gagtcgagga gggcgccctt gcggctctcc
     1681 tccccccgta agcgggcgac gccgccgtcg aagatgtcga agagcccggc cggcaggagg
     1741 aggaggcccg ggaggaacct tcgtccggtg agcagcagca cgccgcagac gatgttcgcg
     1801 atgagggaga gcatcgtgag ctgccacggt cggacgccga tccggtagag cacgtagagg
     1861 acgacgcggt acggccactg gaacagccac cggaagagcg ggccgatgac gctggggttc
     1921 tctcgaggcg gcggtaggtc gcgaacgcgg gtcccggatc gatctggatc ggtcgaatcg
     1981 gcgcgcttca cgacggctac ctgcagtatc cgccaggcgg cccccgggtc gccaccgtcg
     2041 ttt
//
LOCUS       DASZOB010000052         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_182855, whole genome shotgun sequence.
ACCESSION   DASZOB010000052 DASZOB010000000
VERSION     DASZOB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182855"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /locus_tag="VG709_00815"
     CDS             complement(<1..>1533)
                     /locus_tag="VG709_00815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWC31650.1"
                     /translation="LRVLRKVEAIVREEMDAKGAQEVRMPAILPSEPWKVSGRWQAYA
                     AEQLMFVLHDRHGRELGLGPTHEEVVTPLVDADYASYKDLPVNLYQVQWKYRDEARPR
                     SGLVRAREFLMKDAYTFDRDVEGLRVAYAKMVDAYNRVFTRCGLDFRMVEADPGLIGG
                     DVNHEFMAPSPIGEDLFVRCTDCDYAANVEAATCRPLVDAPVEDAPPVEEVSTPGRAT
                     IESVAELLGVPHERVLKCLLYRLGADVVAVLVPGNRDVNDGKLRRLFAPREVEMLTDA
                     DFEEAGLAKGYVGPQGLEGIEIVADPTVREHRSWVAGANRRDAHTTGVTLGRDFDVDR
                     WEDVAGVLPGDRCPRCDTGTLEIDRAIEVGHTFQLGTRYSEPLKATFVDEDGIERPFV
                     MGCYGIGVSRIVASVAEQHHDEAGLAWPRALAPFEVLVVPTNMDRGDVVDAAERIYEE
                     LRARDTEVALDDRAASAGVKFADADLVGYPLQVVVGARGLERGVVEVKVRSSGDRREL
                     PLE"
BASE COUNT          216 a          574 c          519 g          224 t
ORIGIN      
        1 ctccaacggg agctcgcggc ggtcgccgct cgagcgcacc ttcacctcga cgacgccccg
       61 ctcgagcccg cgcgcgccga cgacgacctg cagcgggtag ccgacgaggt cggcgtcggc
      121 gaacttcacg cccgccgagg ccgcgcggtc gtcgagcgcc acctctgtgt cgcgcgctcg
      181 cagctcctcg tagatgcgct cggccgcgtc cacgacgtcg ccacgatcca tgttcgtcgg
      241 gacgacgagg acctcgaacg gcgccagcgc ccgcggccac gcgagccccg cttcatcgtg
      301 gtgctgctcg gcgaccgacg cgacgatgcg cgagacgccg atcccgtagc accccatcac
      361 gaacggccgc tcgatgccgt cctcgtcgac gaacgtcgcc ttgagcggct cggagtaccg
      421 ggtcccgagc tgaaacgtgt ggccgacctc gatcgcgcga tcgatctcga gcgtcccggt
      481 atcgcaccgc gggcagcggt cgccgggcag gacacccgcg acgtcctccc accggtcgac
      541 gtcgaagtcg cggccgagcg tcacgccggt cgtgtgggca tcgcgccggt tcgcgccggc
      601 cacccagctg cggtgctcgc gcaccgtcgg gtcggcgacg atctcgatcc cctcgagacc
      661 ctggggtccg acgtatccct tggcgaggcc tgcctcctcg aagtctgcgt cggtgagcat
      721 ctcgacctcc cgcggcgcga acagccgccg gagcttcccg tcgttgacgt cacggttgcc
      781 gggcacgagc accgcgacga cgtcggcgcc gagccggtac agcaggcact tcaggactcg
      841 ctcgtggggc acgccgagca gctcggcgac cgactcgatc gtcgcgcgtc ccggcgtcga
      901 gacctcctcg acgggcggcg catcctctac cggcgcgtcg acgagcggac ggcacgtcgc
      961 ggcctcgacg ttcgccgcgt agtcgcagtc ggtgcagcgg acgaaaaggt cctcgccgat
     1021 cggcgacggc gccatgaact cgtggttgac gtcgccgccg atgagccccg ggtccgcttc
     1081 gaccatccgg aagtcgagcc cgcatcgcgt gaagacacgg ttgtaggcgt cgaccatctt
     1141 cgcgtacgcc acccgcagcc cttcgacgtc gcggtcgaac gtgtaggcgt ccttcatcag
     1201 gaactcgcgc gcgcgcacga gccccgagcg cgggcgcgcc tcgtcccggt acttccactg
     1261 gacctggtag aggttcacgg ggaggtcctt gtacgaggcg tagtcggcgt cgacgagcgg
     1321 cgtcacgacc tcctcgtgcg tggggcccag gccgagctcg cggccgtggc ggtcgtgcag
     1381 cacgaacatc agctgctcgg ccgcgtacgc ctgccagcgg ccggagacct tccacggctc
     1441 cgaagggagg atcgcgggca tccggacctc ctgggcgccc ttcgcgtcca tctcctcgcg
     1501 gacgatggcc tcgaccttgc ggagcacccg gag
//
LOCUS       DASZOB010000053         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_71788, whole genome shotgun sequence.
ACCESSION   DASZOB010000053 DASZOB010000000
VERSION     DASZOB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71788"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VG709_00820"
     CDS             <1..441
                     /locus_tag="VG709_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M36"
                     /protein_id="HWC31651.1"
                     /translation="HLIRRVQVSAMLRPPNGQDPNDPGSQNRFSALRQFEILTCQRTS
                     AVRCDTPAQFTTIYTSPPDAFPSIAPRPRAPELITRSFRVPATKATFVQLRVLTNQCT
                     GAPDYQGEQDNDPLNDTDCDEAVVTITSPQDQIVRAAELQVFSN"
     gene            487..984
                     /locus_tag="VG709_00825"
     CDS             487..984
                     /locus_tag="VG709_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31652.1"
                     /translation="MPGRAVRGRLRLALTAAAAVWLALTLALGVSRTLRWTDLGRIDD
                     VDGVAYLSERRVFVVRRGNAVLVLSAISPHRPEIGEPLFYCPSGYFVGPHGEIFDQLG
                     RYIDGPAPTGMDRFQAKVEDGFVLADLRDMITGPARSSRGSLPAGRQCILDDGITEPR
                     ILPAP"
     gene            1040..2398
                     /gene="pafA"
                     /locus_tag="VG709_00830"
     CDS             1040..2398
                     /gene="pafA"
                     /locus_tag="VG709_00830"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HWC31653.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDNVLDLVAHDKAGERVLEALLAAAEVRLHEEGI
                     SGDVYLFKNNTDSAGNSYGCHENYLVARRGEFARMAEVLIPFFVTRQIFAGAGKLLHG
                     PRGAQFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEY
                     TTFLKVGVTDLVLRMVEENTVIRDLTLENPIRAIREISHDIQCRKKVRLANGREMSAL
                     DIQEQYLERATRFAEHRGLEPPEKQILRDWEDALEALRTGALDRLASRIDWVAKYLLI
                     QAYRKKHQLPLSHPKVALLDLAYHDVNRKRGLYYVLERRGEMERAVSERDVQRAMREP
                     PQTTRARLRGEFIRHAKQRRRDYTVDWVHLKLNDQAQRTVLCKDPFRSHDERVDQLIA
                     QM"
     gene            complement(2402..3073)
                     /locus_tag="VG709_00835"
     CDS             complement(2402..3073)
                     /locus_tag="VG709_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31654.1"
                     /translation="MRAPVLDVTFNASGAAAAGAAGRVVVIVDVVDSSTSAEAALASG
                     AMEVLGAAPAGVSPPADVDPAAVGRRAATLAARAGTDVVVVAEPRVGTDNERRHRALP
                     VLQALRTASIEYELVPNQGAELPRLIDLDGRIVVVISATGGTAFDAALAGGAPAVTFA
                     TSARSQGRTGWEVLEDGAKRAIKLAREHRAGLTIAAATANSADDVVAAFELARAILSE
                     GFLRI"
     gene            complement(3070..>3500)
                     /locus_tag="VG709_00840"
     CDS             complement(3070..>3500)
                     /locus_tag="VG709_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HWC31655.1"
                     /translation="VASAMSLNAVGILGGRAIGTLRGNFADPAYRHFGVSPHSVTALR
                     DVALVPIHVAVPVLEGDERDAVWTALKEAGLEERHQLVEVTGEPALALLRDRGVRAET
                     MGRSMDEERAFFLAAGAAGVLAGRMASGTRTWRPREGGRP"
BASE COUNT          556 a         1268 c         1187 g          489 t
ORIGIN      
        1 cacctgatca ggcgcgtcca ggtgagcgcg atgctgcgtc cgccgaacgg ccaggacccg
       61 aacgatcccg gaagccagaa ccgcttctct gcgctgcggc agttcgagat cctgacgtgc
      121 cagcggacga gcgcggtcag gtgcgacacg ccggcgcagt tcacgacgat ctacacgagc
      181 ccgccggacg ctttcccgtc gatcgcgcca cggccgcgcg ctcccgagct catcacgcgg
      241 tccttccgcg tgccggcgac gaaggcgacg ttcgtgcagc tccgcgtgct cacgaaccag
      301 tgcacgggcg cgcccgacta ccagggggaa caggacaacg acccgctgaa cgacaccgac
      361 tgcgacgagg cggtcgtaac gatcacgtcg ccgcaggacc agatcgtccg agccgcggag
      421 ctccaggtgt tctccaattg agcggcctct gaacgtggcg gtcggccggt aggggtagcg
      481 cgggacgtgc ccggtcgtgc cgtccgcggg cgactgaggc tcgcgctgac cgccgccgcg
      541 gcggtgtggc tggccttgac cctcgcgctc ggtgtctcgc ggacgcttcg ctggaccgac
      601 ctcggacgga tcgacgacgt cgacggcgtc gcctacctct cagagcggcg cgttttcgtc
      661 gtccggcgcg ggaacgcggt gctggtgttg tcggcgatca gcccgcaccg tcccgagatc
      721 ggcgagcctc tcttctactg cccgagcggc tacttcgtcg gaccccacgg cgagatcttc
      781 gaccagctag gcagatacat cgacgggcct gcgccgacgg gcatggatcg gttccaagcc
      841 aaggtggagg acggcttcgt cctcgcagat ctccgggaca tgatcaccgg tccggctcgc
      901 tcctcacgcg gttctctgcc ggccggtcga cagtgcatcc tcgacgacgg gataacggaa
      961 ccgaggatcc ttcccgcgcc ctagggaagc cagatgcacg accctcggta ttcgaggtcg
     1021 ctccctctag actggccgcg tggagcggcg catcttcggc ctcgagaacg agtacggcgt
     1081 cacgtgcacg ttccgcgggc agcggcggct ctcgcccgac gaggtcgcgc ggtacctctt
     1141 ccgccgtgtc gtccactggg gccggtcgtc gaacgtcttc ctcgagaacg gcgcccgcct
     1201 gtacctcgac gtcggctccc atcccgagta cgcgaccccc gagtgcgaca acgtcctcga
     1261 cctcgtcgcg cacgacaagg cgggggagcg cgtgctcgag gcgctcctgg ccgccgcgga
     1321 ggtgcgcctg cacgaggagg ggatctccgg cgacgtctac ctgttcaaga acaacaccga
     1381 ctccgccgga aactcctacg ggtgccacga gaactacttg gtcgcgcgac gaggcgagtt
     1441 cgcgcggatg gcggaggtgc tgatcccgtt cttcgtcacg cgccagatct tcgccggcgc
     1501 cgggaagctg ctgcacggcc cgcgcggcgc ccagttctgc ctgtcgcagc gcgccgagca
     1561 catctgggag ggcgtctcgt cggcgacgac gaggtcccgt cccatcatca acacccgcga
     1621 cgagccgcac gccgacgcgg agcgctaccg gcgcctccac gtgatcgtcg gcgactccaa
     1681 catgagcgag tacacgacgt tcctgaaggt cggggtgacc gacctcgtgc ttcgcatggt
     1741 cgaggagaac acggtgatcc gcgacctcac gctcgagaac cccatccgcg cgatccgcga
     1801 gatcagccac gacatccagt gccgcaagaa ggtgcgcctc gccaacgggc gcgagatgtc
     1861 ggcgctggac atccaggagc agtacctcga gcgcgcgacc cggttcgccg agcaccgcgg
     1921 gctcgagccg ccggagaagc agatcctgcg tgactgggag gacgcgctcg aggcgctacg
     1981 gaccggtgcc ctcgaccgcc tcgcgagccg catcgactgg gtcgcgaagt acctgctgat
     2041 ccaggcctat cggaagaagc atcagctgcc gctctcgcac cccaaggtcg cgctgctgga
     2101 tctcgcctac cacgacgtca ataggaagcg aggcctgtac tacgtgctcg agcggcgtgg
     2161 cgagatggag cgcgcggtgt cggagcggga tgtccagcgc gcgatgcgcg agccgccgca
     2221 gacgacgcgc gcgcggctcc gcggcgagtt catccggcac gcgaagcaac gacggcgcga
     2281 ctacaccgtg gactgggtcc acctgaagct gaacgaccag gcccagcgga cggtgctctg
     2341 caaggacccg ttccgctcgc acgacgagcg cgtcgatcag ctcatcgcgc aaatgtagcg
     2401 atcagatccg caggaatccc tcggagagga tcgcgcgcgc gagctcgaag gccgccacga
     2461 cgtcgtccgc cgagttcgct gtcgccgcgg cgatcgtgag cccggcccgg tgctcgcgcg
     2521 ccaacttgat cgcgcgcttg gccccgtcct cgaggacctc ccagccggta cggccttgag
     2581 agcgggccga cgtcgcgaac gtgacggccg gcgcgccgcc ggcgagcgcg gcgtcgaacg
     2641 cggttccgcc ggtcgccgag atcaccacca cgatacgtcc gtcgaggtcg atgaggcgag
     2701 gcagctccgc gccctggttc ggcacgagct cgtactcgat gctcgccgtc cgaagcgcct
     2761 ggaggaccgg aagcgcgcgg tggcgacgct cgttgtcggt cccgacgcgg ggctccgcca
     2821 ccacgacgac gtccgttccg gcccgcgcgg cgagggttgc cgcgcgcctt ccgacggccg
     2881 ctggatcgac gtcggcgggc ggcgagacgc cggccggggc agcgcccaac acctccatcg
     2941 cacccgaagc cagtgccgcc tccgcgctcg tcgaggagtc gacgacgtcg acgatcacga
     3001 cgacgcgtcc cgccgcgccg gccgccgcgg cgccgctcgc gttgaacgtc acgtcgagga
     3061 ccggcgccct cacgggcggc cgccttcccg cggccgccac gttcgcgtgc ccgaggccat
     3121 ccggccggcc aggacgcccg ccgcccccgc cgcgagaaag aaggcgcgct cctcgtccat
     3181 cgagcggccc atcgtctcgg cacggacacc gcggtcgcgg agcagcgcga gcgccggctc
     3241 gccggtgacc tccacgagct gatggcgttc ctcgagcccg gcctccttga gcgcagtcca
     3301 caccgcgtcg cgctcgtcgc cctcgagcac cgggacggcg acgtggatcg ggacgagggc
     3361 aacgtcacgg agcgccgtca ccgaatgcgg agagacaccg aaatggcggt acgcgggatc
     3421 ggcgaaattc ccccgcagcg tcccgattgc acggccgccg aggatgccga ccgcgttcag
     3481 cgacatcgca ctcgcgacgt
//
LOCUS       DASZOB010000054         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51483, whole genome shotgun sequence.
ACCESSION   DASZOB010000054 DASZOB010000000
VERSION     DASZOB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51483"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VG709_00845"
     CDS             complement(<1..814)
                     /locus_tag="VG709_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC31656.1"
                     /translation="MIRTEALVKRYGKVTALDGLDLAVPRGTVLGLLGPNGAGKTTAV
                     RILSTLLDPDGGRAIVAGLDVVADATKVRERIGLSGQSAAVDEHLTGYENLEMVGRLY
                     HLGRERSRDRARDLLARFELDDAKNRPAKTYSGGMRRRLDLAAALVAEPDVIFLDEPT
                     SGLDPRSRLQMWGTIEELVGGGSTVLLTTQYMEEADRLADDIVVIDRGRKIAQGTSDA
                     LKTQVGGERIEVIVHDRADIPRAAELLARDCAGDCSIEEHTRRLTVPANGGAQ"
     gene            complement(1210..1962)
                     /locus_tag="VG709_00850"
     CDS             complement(1210..1962)
                     /locus_tag="VG709_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWC31657.1"
                     /translation="MTDAHSTDDASSDRFVPTVSKEVAERIRAWHERAYREGAGRSTD
                     TFHYLGFSLVVPPDVQPITPMSHLLGEAVLAEVRPGERVLDMGTGSGVNAVLAASKGA
                     RVVAVDINPHAVQAARDNAQRNDVSHLIDARQSDVFSAVDEVFDLIVFDPPFRWFRPR
                     DLREAATTDENYRAMTRFFRGARQHLTRDGRMLISFGTSGDLGYLKQLFEKGGFQYEE
                     VARAAIVRDDWQVDYFTFRCSIDAAVDERERS"
BASE COUNT          315 a          708 c          651 g          348 t
ORIGIN      
        1 gttgcgcgcc accattcgcg ggcactgtca gacggcgcgt gtgctcctcg atcgagcagt
       61 cgccggcaca atcgcgggcc agcagctcgg ccgcgcgagg aatgtcggcg cggtcgtgca
      121 cgatcacctc gatccgctcg ccgccgacct gcgtcttcag cgcgtcggac gtgccctgag
      181 cgatcttccg gccccggtcg atcacgacga tgtcgtcggc gagacggtcc gcctcctcca
      241 tgtactgcgt cgtgagcagg acggtcgacc cgccgccgac gagctcctcg atcgtccccc
      301 acatctggag ccggctccga gggtcgagcc ccgacgtcgg ctcgtcgagg aagatcacat
      361 cgggctccgc gaccagcgcg gctgcgaggt ccagacgccg ccgcatcccg ccggagtacg
      421 tcttggccgg acgattcttc gcgtcatcca gctcgaaccg cgcgaggagg tcgcgggcgc
      481 gatcgcgcga cctctccctt cccaggtggt agagacggcc gaccatctcg aggttctcgt
      541 aaccggtcaa gtgctcgtcg acggcggcgg actggccgga gagcccgatg cgctcgcgca
      601 ccttcgtggc gtcggccacg acgtcgagcc ccgccacgat cgcccgcccg ccgtcgggat
      661 cgaggagcgt gctgaggatc cggacggccg tggtcttgcc ggcgccgttc ggacccagca
      721 gcccgagcac ggtccctcgc ggcaccgcga gatcgagccc gtcgagcgcc gtgacctttc
      781 cgtatcgctt gaccagcgcc tcggtgcgga tcatctcgtc caaccgacgt ctccctcttc
      841 cgtcggcctc gggcgtggcc ggctcgcatc tccaaccgcg gacgcggggc gcgtcttccc
      901 gcgaagccgc gagcataccg gccggccccc ccggatgtca gagggcgcga acctacgatc
      961 gcgaagccgt cgaggcgagc gtcgcgcgtt cgagcagcgc ttccagctcg cgggatcacc
     1021 cgttccggga gaaggctctc ccccgccgtg cgcgagttcc tggcggatct cgagcgacat
     1081 ctgcatgcgg tcgccgagga gttcgaccgt ctgcggtgac ggtctggctg cagcttcgga
     1141 aacccgctgt cgttggatac tgcgcacgat gttgcggcgg cggccagtcg tccgatgtcg
     1201 cgtccgaccc tacgaccgct cgcgctcgtc gaccgcggcg tctatcgagc accggaacgt
     1261 gaagtaatcg acctgccagt cgtccctcac gatggcggcg cgagccacct cttcatactg
     1321 aaaccctccc ttttcgaaga gctgcttgag gtacccgagg tcgcccgacg tcccgaagga
     1381 gatcaacatc cttccgtcac gcgtcaagtg ctgcctggcc ccccggaaga acctcgtcat
     1441 cgcacggtag ttctcgtcgg tcgtagcagc ctcccggaga tcccttgggc ggaaccagcg
     1501 gaagggcgga tcgaagacga tgaggtcgaa cacctcgtcg acggcggaga acacgtcgct
     1561 ttgtcgagcg tcaatcagat ggctgacgtc gttgcgttgg gcgttgtcgc gtgccgcctg
     1621 gacggcatgt ggattgatgt ccaccgcgac cacgcgagct cccttcgacg cagccaggac
     1681 ggcgttgact ccacttccgg ttcccatgtc caggactcgc tcgccgggtc gcacctctgc
     1741 gagtaccgcc tcgccgagca ggtgcgacat cggcgtgatg ggctggacat cgggcggcac
     1801 gacgagggaa aagccgagat agtggaacgt atccgtggag cgtccagcgc cctcgcgata
     1861 ggctcgctcg tgccaagctc gaatccgctc cgcgacttcc ttcgatacgg tcggcacgaa
     1921 cctgtcagat gatgcgtcgt ccgtcgaatg agcgtctgtc atctctatgt cgctgccgac
     1981 ccgacggtca agatggacac taccgtaggg atgtcgatcg cc
//
LOCUS       DASZOB010000055         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_172166, whole genome shotgun sequence.
ACCESSION   DASZOB010000055 DASZOB010000000
VERSION     DASZOB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172166"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..531)
                     /locus_tag="VG709_00855"
     CDS             complement(166..531)
                     /locus_tag="VG709_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC31658.1"
                     /translation="MVAVRSLDHIYYWVTDMDRAVTFYEEVLGLKLLRRDGGNWAEFD
                     ADPVRLALHGAVEGHPVEPGGATVSFRVDDVEAARADLEARGVEIDHAGEVGTWARYA
                     TFRDPDGNTVQVIEYAEEP"
     gene            complement(533..1699)
                     /locus_tag="VG709_00860"
     CDS             complement(533..1699)
                     /locus_tag="VG709_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWC31659.1"
                     /translation="MGDRVAIVGVGHAGFAPITAGLSYKELTFEAAMRAYDDAGINPR
                     KDVDQFVAVSEDFWEGTSIFDEYVPDQIGAALRPVHTVTADGLFGIAAAVMLIRSGLA
                     EVVAVEGHSKASDILTLGHIHQFAMDPVLNRPLDVSPLAVAGLEMNRYLEATGQTDEH
                     CARVVEKNRTNALDNAHAAYPTRVAAKDVAVSEPLWWPLRRLDVSQRADGCVVLVLAS
                     GDRALDLTDDPVWIAGIGWSSGTPTLESRSWEEADYVRDAANRAYEMAGVTAPSSDID
                     IAEVDDTYSYRELMHLEALRLARPGDAGPMLDEGFFGRDGDLPVNVSGGSLGQGYLFE
                     ANGLARTLECVLQLRGEAGERQVDEADVALAQSWRGVPTTSAAVAVLASDEVVA"
     gene            complement(1748..2389)
                     /locus_tag="VG709_00865"
     CDS             complement(1748..2389)
                     /locus_tag="VG709_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC31660.1"
                     /translation="MVDPEFVRRRTTEAALPDGTRIRLRPIVPEDKALLVAGFERMSP
                     ESRYRRFMAPLEELTPELLVRLTEVDYRNHFAWLALALDDPLTPGAGVARYVRLPDEP
                     EVAEAAVTVIDDYHGRGIGTLLLQALGAVALENGIRRFRSYAVEANVPLRELMHNLGA
                     HVEHDSPGLVRVEVDLPARAEELKGTPLYEVLRAVARGEGPRFLPAGLDAARS"
     gene            complement(2392..2991)
                     /locus_tag="VG709_00870"
     CDS             complement(2392..2991)
                     /locus_tag="VG709_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWC31661.1"
                     /translation="MGFPKRLLSEDEELVLDIRPHWIALLLPLLVAALLVALEVLVLV
                     NIPDRWPDWVRWAVVAAGVVFFLAVPARRIIAWATSHFVVTSERVIHRSGWLARRAME
                     MPLDRINDVRFEQSVLERIVGGGDLVIESGGEYGQNHFTDIRKPEDVQKLIYETAEVE
                     ESGDRPPLDEIERLAALKERGAISEEEFETHKRRLLGRL"
     gene            complement(2998..3207)
                     /locus_tag="VG709_00875"
     CDS             complement(2998..3207)
                     /locus_tag="VG709_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31662.1"
                     /translation="MEDGAMTRYADLPLNEIEEPPERYGRPRLDAEHDLPADVAELRD
                     AVGGLSAVTARTLMPLDAGLAARAD"
     gene            complement(3322..4113)
                     /locus_tag="VG709_00880"
     CDS             complement(3322..4113)
                     /locus_tag="VG709_00880"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWC31663.1"
                     /translation="MGKLGQDSVARAAAAAAIDVAPTFLETTRSTNADAVALAERGAP
                     EWTLVAAGEQTAGRGRLGRSWASAPGKALLFSVVFRPPLEPARAAIVSHLAAAVMAEA
                     CGPPRAVRTKWPNDLVVGERKLAGVLPEAKVVGGALDYLVVGVGVNVSMDRDDFPPDV
                     RDTATSLALEGLEADADALLTRFLVSFRAAYRPIDPAFPQHAAAEYAAACATLRRPVR
                     ARTIDGDVVEGVATRITDSGALVVRTDDGERDVAFGEVEHLRSRR"
     gene            4196..>4563
                     /locus_tag="VG709_00885"
     CDS             4196..>4563
                     /locus_tag="VG709_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWC31664.1"
                     /translation="MRTCRELGIPTVAVYSDADREALHVETADRAEHIGPATPAESYL
                     NVPAILEAARRSGATAVHPGYGFLAENPEFARACAAAGLTFIGPPPEAMEMMGDKAAA
                     RRAAEKIGVPVVPGVSEPVGV"
BASE COUNT          661 a         1656 c         1623 g          623 t
ORIGIN      
        1 cgtcgggggc ggacccgagc gcgacggagt cgacgtcgtc gagcgtcatc tcgcaggaat
       61 cgagggccgc cttcgcggcc tgccatgaga gctccttgcc ggtctcctga gcacggcgca
      121 cgaacttcgt catgccggcg ccgacgaccg cgacacgctg tcccactagg gctcctccgc
      181 gtattcgatg acctggaccg tgttgccgtc cggatcgcgg aacgtcgcgt agcgcgccca
      241 cgtccccacc tcgccggcgt ggtcgatctc gacgccgcgc gcctcgaggt cggcgcgcgc
      301 ggcctcgacg tcgtccacgc ggaacgacac ggtcgcgccg ccgggctcga cgggatggcc
      361 ctcgaccgcc ccgtgcagcg ccaggcggac ggggtcggcg tcgaactcgg cccagttgcc
      421 gccgtcgcga cgcaggagct tgagcccgag gacctcctcg tagaacgtca cggcgcggtc
      481 catgtcggtg acccagtagt aaatgtgatc gagcgagcga accgcgacca cgtcacgcca
      541 ccacctcgtc ggacgccagc acggcgacgg ccgcggacgt ggtcggcacg ccgcgccacg
      601 actgcgcgag cgcgacgtcg gcctcgtcca cctgccgctc gccagcctct ccccggagct
      661 ggagcacgca ctccagcgtc cgcgcgagcc cgttggcctc gaagaggtat ccctggccga
      721 gcgagccgcc cgagacgttt acgggtaggt ctccgtcccg gccgaagaac ccctcgtcga
      781 gcatcggccc ggcgtctccc ggacgcgcga ggcgcagcgc ctcaaggtgc atcagctcgc
      841 ggtacgagta ggtgtcgtcg acctcggcga tgtcgatgtc cgacgacggc gccgtgacgc
      901 ccgccatctc gtacgcgcgg ttggcggcgt cgcggacgta gtcggcctcc tcccacgaac
      961 ggctctcgag cgtcggcgtg ccggaggacc agccgatccc ggcgatccac accgggtcgt
     1021 cggtaaggtc gagcgcccgg tcccccgacg cgaggaccag cacgacgcat ccgtcggcgc
     1081 gctgcgagac gtcgaggcgc cgaagcggcc accagagagg ctccgagacg gccacgtcct
     1141 tggccgcgac gcgcgtcggg taggccgcgt gtgcgttgtc gagcgcgttc gtgcggttct
     1201 tctcgacgac gcgcgcgcag tgctcgtcgg tctggccggt ggcctcgagg tagcggttca
     1261 tctcgaggcc ggcgaccgcg agcggcgaca cgtcgagcgg gcggttcagc accgggtcca
     1321 tcgcgaactg gtggatgtgg ccgagcgtga ggatgtccga ggccttgctg tgtccctcga
     1381 ccgcgacgac ctccgcgagg cccgaccgga tcagcatcac ggcggcggcg atcccgaaga
     1441 gcccgtcggc cgtgacggtg tggacgggcc ggagcgccgc cccgatctgg tcgggcacgt
     1501 actcatcgaa gatgctggtg ccctcccaga agtcctcgga gaccgcgacg aactggtcga
     1561 cgtccttgcg cgggttgatg ccggcgtcgt cgtaggcccg catcgcggcc tcgaacgtga
     1621 gctccttgta cgagaggcct gcggtgatcg gggcgaagcc ggcgtgaccg accccgacga
     1681 tggcgacccg gtcccccatg ggcgcgcgga gtctagcaaa ggggcagatc gcgcccgaaa
     1741 tcgcgggcta cgatcgcgcc gcgtcgagcc cggcgggaag gaagcgcgga ccctcgccgc
     1801 gcgccacggc gcggaggacc tcgtagagcg gggtcccctt cagctcctcc gcccgcgcgg
     1861 gaaggtccac ttcgactcgg accagccccg gcgaatcgtg ctcgacatgc gcgccgaggt
     1921 tgtgcatcag ctcgcgaagc ggcacgttcg cctcgacggc gtagctgcgg aaccgccgta
     1981 tgccgttctc gagcgccacg gcgccgaggg cctgtagcag caacgtgccg attccccggc
     2041 cgtggtagtc gtcgatcacc gtgacggcgg cctcggcgac ctccggctcg tccggcagcc
     2101 gcacgtatcg cgcgactccc gcaccgggcg tgagcggatc gtcgagggcc agtgccagcc
     2161 acgcgaagtg gttgcggtag tcgacctccg tgaggcggac gaggagctcc ggggtcagct
     2221 cctcgagcgg cgccatgaag cggcggtagc gcgactccgg cgacatccgc tcgaaaccgg
     2281 cgacgaggag cgccttgtcc tctgggacga tcgggcgcag acggatccgc gtgccgtcgg
     2341 ggagcgcagc ctcggtcgtg cggcggcgta cgaactctgg gtcgaccacg gctacaggcg
     2401 cccgaggagc cgccgcttgt gcgtctcgaa ctcctcctcc gagatcgcgc cgcgctcctt
     2461 cagggccgcc aggcgctcga tctcgtccag cggcgggcgg tcaccgctct cctcgacctc
     2521 ggccgtctcg tagatgagct tctgcacgtc ctccggttta cggatgtcgg tgaaatggtt
     2581 ctgcccgtac tcgccgccgg actcgatcac gaggtcgccg cccccgacga tccgctcgag
     2641 gaccgactgc tcgaagcgaa cgtcgttgat ccgatcgagc ggcatctcca tcgcacgccg
     2701 cgccagccac ccggaccggt ggatcacccg ttcgctggtc acgacgaagt gcgacgtcgc
     2761 ccacgcgatg atgcgccgtg ccgggaccgc gaggaagaac acgaccccgg ccgcgacgac
     2821 ggcccaccgc acccagtcgg gccagcggtc ggggatgttc acgaggacca gcacctccag
     2881 cgcgacgagg agcgccgcga cgagcagcgg aagcaggagc gcgatccagt gcggacggat
     2941 atcgaggacg agctcctcgt cctccgacag cagccgcttc gggaatccca tcgcgcgtca
     3001 gtctgcgcga gcggcgagac cggcgtccag cggcatcagc gtccgcgccg tcacggccga
     3061 gagcccgccg accgcgtcgc gcagctccgc gacgtcggcg gggaggtcgt gctcagcgtc
     3121 gagacgcgga cgcccgtacc gctcgggcgg ctcctcgatc tcgttgaggg ggaggtcggc
     3181 gtatcgggtc atcgcaccgt cctccatgac gccctgatcc taggacgacg ctctgaattt
     3241 ccggggggtc cgaagggatt tggctgaaac ttcccaacgt ttccacgccg gccgcccgcg
     3301 aagcgatcgg ccgcggaagc cctaacgccg cgatctgagg tgctctacct cgccgaaggc
     3361 gacgtcgcgc tcaccgtcgt cggtccggac gacgagggcg cccgagtcgg tgatccgcgt
     3421 cgcgaccccc tcgacgacgt cgccgtcgat cgtgcgggcg cggaccggcc gtcgcagcgt
     3481 cgcgcacgcc gccgcgtact cggcggcggc gtgctgcggg aacgccgggt cgatcgggcg
     3541 gtacgccgcg cggaaggaca cgaggaagcg cgtgagcagc gcgtcggcgt ccgcctctag
     3601 gccctcgagg gccaggctcg tcgcggtgtc gcggacgtcc ggtgggaagt cgtcgcggtc
     3661 catcgagacg ttcacgccaa ccccgacgac gaggtaatcg agggccccgc cgaccacctt
     3721 ggcttcgggc agcacgccgg cgagcttacg ttcgccgacg acgagatcgt tcggccactt
     3781 cgtccggacg gcgcgcggcg gaccgcatgc ctctgccatc acggctgccg ccaggtgcga
     3841 gacgatcgcg gcgcgggccg gctcgagcgg cggacggaac acgaccgaga acagcagcgc
     3901 cttgccgggc gccgacgccc acgagcggcc gagccggccc cggcccgccg tctgctcccc
     3961 ggccgccacc agcgtccatt ccggcgcgcc gcgttcggcg agcgcgacgg catcggcgtt
     4021 ggtcgagcgc gtcgtctcca ggaacgtcgg cgcgacgtcg atcgctgcgg cggcggccgc
     4081 gcgcgccacg ctatcctggc cgagcttccc cacgaggcgg catcctaggc ggaggaaacg
     4141 cgtggccgac gtccggatcc tcatcgcgaa ccgcggggag atcgccgtcc ggatcatgcg
     4201 cacgtgtcgc gagctcggga tcccgacggt cgccgtgtac tccgacgccg accgcgaggc
     4261 cctccacgtc gagacggccg accgcgccga gcacatcggg ccggcgacgc cggccgagtc
     4321 gtacctcaac gtgccggcga tcctcgaggc cgcgaggcga tccggcgcga cggcggtcca
     4381 ccccggctac ggcttcctcg cggagaatcc cgagttcgcg cgcgcctgcg ccgcggcggg
     4441 gctcacgttc atcggcccgc cgccggaggc gatggagatg atgggcgaca aggccgcggc
     4501 gcgccgggcc gccgagaaga tcggggtgcc ggtcgtgccg ggcgtctccg agcccgtcgg
     4561 cgt
//
LOCUS       DASZOB010000056         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_71890, whole genome shotgun sequence.
ACCESSION   DASZOB010000056 DASZOB010000000
VERSION     DASZOB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71890"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VG709_00890"
     CDS             <1..512
                     /locus_tag="VG709_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWC31665.1"
                     /translation="RHLTQREHALHDRQRRERLHVPSPSFRGSRGGLAHQRIRIARRR
                     RDRVADAATEHVYPDGTVIAERGDPGDAMHVVVAGKVRVLVDEDGRLVEVASRGPGYI
                     VGEMAVLTDEPRMASLVAAGDVRTLSLDRARFQRILRERPDAAAAVMRELCARLRDVH
                     EHREFEVPR"
     gene            509..901
                     /locus_tag="VG709_00895"
     CDS             509..901
                     /locus_tag="VG709_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006090280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWC31666.1"
                     /translation="MSRRLIASGSPYEPIVGFSRAVVVGRHVAVAGTAPTMPDGGQPP
                     PDAYDQARRCLEIILTALDEAGARAEDVVRTRIFLVRADDWKEVGRAHGEVFAEIRPA
                     NTMIVVEGFLDPRWLVEVEADAIVGPDL"
     gene            898..2034
                     /locus_tag="VG709_00900"
     CDS             898..2034
                     /locus_tag="VG709_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWC31667.1"
                     /translation="MSASVVLVYGRELLAYDHGPQHPLRPVRVMLTRELIASYGMLDG
                     GVEEVAPRAATDDELQLVHTPEYVDAVKRAGSGEPGDWWRYGFGPGDNPIFRGMHEAS
                     SRVAGASLVAAEAVASGTADHAFSPAGGLHHAMPDRASGFCVYDDPAIAIAWLLGSGV
                     ERVAYVDVDVHHGDGPQAIFYRDPRILTISIHESGRYLFPGTGFVDERGAGDAEGTKA
                     NVPLPPYTSDEGWLRAFREIVPPLVRRFSPDVLVTQLGCDTHHTDPLAHLALTTFAYR
                     EAAKLLHELAHEAAGGRWLATGGGGYQWARVVPRAWTLYFAEMAEREVPDAIPQRWLD
                     LARTQVGGDVPGRLSEDAPPARESIDEIAAVVDDVRRTVFGPEP"
     gene            2034..3437
                     /gene="pyk"
                     /locus_tag="VG709_00905"
     CDS             2034..3437
                     /gene="pyk"
                     /locus_tag="VG709_00905"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWC31668.1"
                     /translation="MRRTKLVCTLGPASERVVPDLVRAGMDVARVNCSHGTHEDHDRL
                     VAAVREAEADAARPIAVMADLSGPKVRLGALDDGEVRLDERSTFLLRTEDGAPGTADG
                     APVSHPGLASDLEEGDRILLADGAVELRVVATRAGEVVTEVVRGGLVRSRAGVNVPSE
                     RLGLPTMGAKDRADLDWALAAGVDLVAQSFVRSAEDVDEVRALCAARDVPVVAKVETS
                     AAVRDAARIVRAADALMVARGDLGVETVLEEIPIVQKRLIASARAAGIPSIVATQMLE
                     SMVHAERPTRAEVSDVAGAVFDGADAVMLSAETAIGDHPVPAARTAATIAEVAETEGR
                     SFLPQPIDVESYASDAHAVAEAAAEIARDGAAVAIACFTRTGTTARLLSAARPRVPIL
                     TLAPDERVLRRVVLYHGVVPRPSSTPADTDEMIELLDRGLRETAVAASGERVVLVASS
                     PFGHAHTNLLKLHHCGS"
     gene            3976..4641
                     /locus_tag="VG709_00910"
     CDS             3976..4641
                     /locus_tag="VG709_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWC31669.1"
                     /translation="MFLEGTLPAEDGAALKAALQRASKEIPRDERARDREGARMADAL
                     VALVTSGGSEAARRETTLVIHADAAVLAGAPSAALAETEAGDRLPADAVRRLACEARI
                     DWVLERDGDPVKLGTQGRKLPERLRRALVFRDRGCRFPGCEARRYVDGHHIVHWADGG
                     PTTLDNLVMLCGAHHRLIHEAGWRISGHPDRDLRFHDPTGRRLRAHPEPLARARAPAP
                     LGG"
     gene            4824..5195
                     /gene="rpsL"
                     /locus_tag="VG709_00915"
     CDS             4824..5195
                     /gene="rpsL"
                     /locus_tag="VG709_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWC31670.1"
                     /translation="MPTTEQLVRNGRKRPRSKTKSPALRGSPQRRGVCTRVYTATPKK
                     PNSALRKVARVRLTSGIEVTAYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYKVIRAAL
                     DTAGVGDRKKARSKYGAKKTA"
     gene            5205..>5504
                     /locus_tag="VG709_00920"
     CDS             5205..>5504
                     /locus_tag="VG709_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWC31671.1"
                     /translation="MPRRGAPTRRGIEPDPIYQNVLVTQLINKLMLHGKKTLAERVVY
                     QALDKISERTANDPVITLKKAVDNARPLLEVRSRRVGGATYQVPVEVRPPRGTTLA"
BASE COUNT          763 a         1924 c         2045 g          772 t
ORIGIN      
        1 agcggcacct cacgcagcgc gagcacgcgc tgcatgatcg acagcgtcga gagcgtctcc
       61 acgtcccgtc cccttccttc cgcggctcgc gcggcggtct cgcgcaccag cgcatccgga
      121 tcgctcggcg ccgtcgcgac cgcgtcgccg atgccgcgac cgagcacgtc tacccggacg
      181 gaaccgtgat cgccgagcgc ggcgatccgg gggacgcgat gcacgtcgtc gtcgcgggaa
      241 aggtgcgggt cctggtcgac gaggacggcc ggctcgtcga ggtcgcgagc cgcggccccg
      301 ggtacatcgt cggggagatg gccgttctca ccgacgaacc gaggatggcg tcgctcgtcg
      361 cggcgggcga cgtgcggacg ttgtcgctcg atcgcgcgcg cttccagcgg atcctgcgcg
      421 agcggcccga cgccgcggct gcggtgatgc gggagctctg cgcgcggctg cgcgacgtgc
      481 acgagcaccg cgagttcgag gtgccgcggt gagtcgccgg ctgatcgctt cggggtctcc
      541 gtacgagccg atcgtcggct tctcgcgagc ggtcgtcgtg gggcgccatg tcgcggtggc
      601 gggcacggcg ccgaccatgc ccgatggtgg acagcctccg ccggacgcct acgatcaggc
      661 gcggcgttgc ctcgagatca tcctcacggc gcttgatgag gcgggggcac gcgcggaaga
      721 cgtggtccgg acacgcatct tcctcgttcg cgcggacgac tggaaggagg tggggcgggc
      781 gcacggcgag gtgttcgcgg agatccgtcc agcgaacacc atgatcgtcg tcgaggggtt
      841 cctcgacccg cgatggctcg tggaggtcga ggcggacgcg atcgtcgggc ccgacctgtg
      901 agcgcatccg tcgtactcgt ctacgggcga gagctgctcg cgtacgacca cggtccgcag
      961 cacccgctcc gtcccgtccg cgtgatgctc acgcgcgagc tgatcgcgag ctacgggatg
     1021 ctcgacggcg gcgtcgagga ggtcgcgccg agggccgcga ccgacgacga gctgcagctc
     1081 gtgcacacgc cggagtacgt cgatgccgtg aagcgagcgg gttccggcga gccaggcgac
     1141 tggtggaggt acggattcgg ccccggcgac aacccgatct tccgggggat gcatgaggcg
     1201 tcctcgcgcg tggcgggcgc gtcgctcgtc gctgccgagg ccgtggcgtc gggcacggcg
     1261 gaccacgcct tcagccctgc cggagggctc catcatgcga tgccggatcg cgcgtcgggg
     1321 ttctgcgtct acgacgatcc cgcgatcgcg atcgcatggc tgctcggaag cggcgtcgag
     1381 cgcgtcgcgt acgtcgacgt cgacgtccat cacggcgacg gcccccaggc gatcttctac
     1441 cgtgacccgc gcatcctcac gatctcgatc cacgagtccg gtcggtacct gttcccgggg
     1501 acggggttcg tcgacgagcg gggagccggc gacgccgagg ggacgaaggc gaacgtcccg
     1561 ctgcctccgt acacgtcgga cgaggggtgg ctccgcgcct tccgtgagat cgtcccgccg
     1621 ctcgtccgcc ggttctcgcc cgacgtcctc gtgacgcagc tcggctgcga cacgcaccac
     1681 accgatccgc tcgcccacct cgcgctcacg accttcgcgt accgcgaagc cgcgaagctc
     1741 ctgcacgagc tcgcgcacga ggcggccggt ggcaggtggc tcgccacggg cggcggcgga
     1801 taccagtggg cgcgcgtcgt ccctcgtgcg tggacgctct acttcgcgga gatggccgaa
     1861 cgcgaggtgc ccgacgcgat cccgcagcgg tggctcgatc tcgcgcggac ccaggtgggg
     1921 ggcgacgtac cggggcggct gtcggaggac gctccgcccg cgcgcgagtc gatcgacgag
     1981 atcgcggccg tcgtcgacga cgtccggcgg acggtgttcg gtccggagcc gtagtgcgtc
     2041 gcacgaagct tgtctgcacg cttggcccgg cgagcgagcg cgtcgtgccg gatctcgtcc
     2101 gcgccggcat ggacgtcgct cgggtgaact gctcgcacgg gacccacgag gaccacgacc
     2161 ggctcgttgc ggcggtccgc gaggctgagg cggatgcggc gcggccgatc gccgtgatgg
     2221 ccgatctctc gggaccgaag gtgcggctcg gcgcgctcga cgacggcgag gtacggctcg
     2281 acgagcgctc gacgttcctc ctgcggaccg aggacggcgc gcccgggacg gccgacgggg
     2341 cgccggtctc gcatccgggc ctcgcgtcgg atctcgagga aggcgatcgc atcttgctcg
     2401 ccgacggcgc cgtggagctc cgcgtcgtcg cgacgcgtgc cggcgaggtc gtcaccgaag
     2461 tcgtacgcgg cggtctcgtg cgttcgaggg cgggagtcaa cgtgccgtcc gaacgcctcg
     2521 ggttgccgac gatgggggcg aaggaccgag cggatctcga ctgggcgctc gcggccggcg
     2581 tcgacctcgt cgcgcagtcg ttcgtgcgca gcgccgagga cgtcgacgag gtgcgcgcgc
     2641 tgtgcgctgc gcgcgacgtg ccggttgtcg cgaaggtcga gacgtcggcg gccgtgcgcg
     2701 acgccgcgcg catcgtgcga gcggccgacg cgctcatggt cgcgcgcggc gatctcggcg
     2761 tcgagaccgt cctcgaggag atcccgatcg tccagaagcg cctgatcgcg agcgcgcgcg
     2821 ccgccggcat cccgtcgatc gtcgcgacgc agatgctcga gtcgatggtc cacgccgagc
     2881 gccctacgcg tgcggaggtc agcgacgtgg ctggagcggt cttcgacgga gccgacgccg
     2941 tgatgctgtc agccgagacc gcgatcggcg accaccccgt gcccgcagcg cgcacggccg
     3001 cgacgatcgc cgaggtggcc gagaccgagg ggcgttcgtt cctgccgcag ccgatcgacg
     3061 tcgagagcta tgcgagcgac gcgcacgccg tcgcggaggc cgccgcggag atcgcccgcg
     3121 acggcgccgc ggtggcgatc gcgtgcttca cgaggacggg gacgacggcg cgcctcttgt
     3181 ccgcggctcg ccctcgcgtt ccgatcctca cgctggcgcc ggacgagcgc gtcctccgcc
     3241 gcgtcgtcct ctaccacggc gtcgtccctc ggccatcgtc gacgccggcc gatacggacg
     3301 agatgatcga gctgctcgac cgcgggctgc gggagacggc ggtcgccgct tccggagagc
     3361 gcgtcgtcct ggtcgcgtcg tcgccgttcg gccacgcgca cacgaacctg ctgaagctcc
     3421 accactgcgg ttcctagccc ctcacggtcc cggccgtttt ccgcctctga cctgcggtca
     3481 tgtcacaccc tccggctacg atcgcggtcc tggagatgga cgtccgcgcc gaagccgacg
     3541 ccctcgccgg ggccctcgac cgcgtcgccc tcgccgaacc gagctcgccg cggagaaact
     3601 ccggctgatc gctgactacg acgagcgccg gctctggaag ctcgacggtg cgagatcgat
     3661 ggccgactgg ctcgccgccc gctacgggac cgcgaaggtc accgcttcgg agtcggttcg
     3721 cgtcggggag gcgctgcgga cgcttccccg gatcgcccag gcgcacgggg cgcctgtcct
     3781 gggatcagct caagcccctc acgaggttcg ttcgtcccga cgaggacgag gagtgggcgg
     3841 agcgcgcgcc tcggtggcgc ccctcctccc tgtggcgcga agagcggcgc cggcgccggc
     3901 tcgaggagca tgtcgtcgcc gaggcgcggc ggctccgctc gttcgcgatg tggcgggacc
     3961 tcgaggggca cgttgatgtt cctcgagggg acgcttcccg cggaggacgg cgccgccctc
     4021 aaggcggcgc tccagcgcgc ctcgaaggag atcccgcggg acgagcgggc gcgggatcgc
     4081 gaaggcgccc ggatggccga cgcgctcgtc gcgctcgtca cgagcggtgg atcggaggcc
     4141 gcgcggcggg agacgacgct ggtcatccac gccgacgcgg ccgtgcttgc cggcgccccc
     4201 tcagccgcgc tcgccgagac cgaggccgga gaccgcctgc cggccgacgc ggtgcggcgt
     4261 ctggcgtgcg aggcgcggat cgactgggtc ctcgagcgcg acggcgaccc ggtgaagctc
     4321 gggacccagg gccggaagct ccccgagcgg ctccggcggg cgctcgtctt ccgggaccgg
     4381 ggatgccggt tccccgggtg cgaggcgagg cgctacgtcg acgggcatca catcgtccac
     4441 tgggccgacg gtggtcccac gacgctcgac aacctcgtga tgctgtgcgg cgcccaccat
     4501 cggctgatcc acgaggcggg atggcggatc tccggccacc ccgaccgcga cctccgattc
     4561 cacgacccga ccggaaggcg gctgagggca catcccgagc cgctcgcgag ggcgagggcg
     4621 ccggcgcccc tcggaggcta gccgtacagc ggcatgcgcg gcgttgaccg cccgcgcggt
     4681 tcgctataat ctccttcggt tgtggtcgga agcggccaca catccattct gcgtaccggg
     4741 gaccgctgcg aagagattcc agctcggggg ctcgttgcgg ctgcccctgc gcaggagcga
     4801 gacgtgacga gactgggaca gagatgccga cgacggaaca gctggtcagg aacgggcgca
     4861 agcgcccgcg gagcaagacg aagagccccg cgctccgggg ctctccgcag cgccgcggcg
     4921 tctgcacgcg cgtctacacg gcgacgccga agaagccgaa ctcggcgctt cggaaggtcg
     4981 cccgcgttcg cctgacgtcg ggcatcgagg tcaccgcgta catccccggc atcggccaca
     5041 acctccagga gcactcgatc gtcctcgtcc gtggaggccg cgtgaaggac ctcccgggcg
     5101 ttcggtacaa ggtgatccgg gccgctctcg acactgccgg cgtcggcgac cgcaagaagg
     5161 ctcgctccaa gtacggcgcg aagaagacgg cgtagggggt cgggatgccg aggcgtggag
     5221 ctccgacgag gcgcgggatc gagccggacc cgatctacca gaacgtgctg gtcacccagc
     5281 tcatcaacaa gctgatgctg cacgggaaga agacgctggc cgagcgggtt gtatatcagg
     5341 cgctcgacaa gatctccgag cgtaccgcca acgacccggt gatcacgctg aagaaggcgg
     5401 tcgacaacgc tcggccgctg ctcgaggtgc gatcccggcg ggtcggtggc gcgacgtacc
     5461 aggtgccggt ggaggtgcgg ccgccgcggg gcaccaccct ggcg
//
LOCUS       DASZOB010000057         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131806, whole genome shotgun sequence.
ACCESSION   DASZOB010000057 DASZOB010000000
VERSION     DASZOB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131806"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VG709_00925"
     CDS             complement(<1..596)
                     /locus_tag="VG709_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWC31672.1"
                     /translation="MRLPVRPPLPPMLARLERELPHDRYLYEPKWDGFRALAFRDGDA
                     VDIRSRNDRPLARYFPELVAGIRSLRDDAVALDGEIVVLTDGAFDFAAIMARLHPAAS
                     RVERLRRETPARFVAFDLLAAAGEDLRAAPFVERRAALEALAAPADGPLVVTPVTDDP
                     KVAEGWLERFCGGGVDGVVAKHRDLVYQPGKRAMVKVK"
     gene            740..>2017
                     /locus_tag="VG709_00930"
     CDS             740..>2017
                     /locus_tag="VG709_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWC31673.1"
                     /translation="MKRAVLSLVVALAAAALLSPAAAAGKAGRYIVVLRASADAPTVA
                     AQHARSHGAAVSHVYEHALRGYAARLGGAAVARIERDARVAYVEPDLRVHAFAQVLPT
                     GVDRIDGELSPTAKIDGLDDDAVGSVERVNVDVAVIDTGVQFNHPDLNVVAMTDCTGG
                     GPFRQKCTDGKGEDQNGHGTHVAGTIGALDNGIGVVGVAPGARIHNVRVLDSRGSGYT
                     SWIIAGIDWVTARASTIEVANMSLGGSGYTAAQHTAVKNSVAKGVVHVVAAGNSARDV
                     YGPDGRFGSSDDSQPASYPEAMTISALADADGRSGGLAATSTTFSICTENRDDSFACF
                     SNFSRSVVAGNPVVSTGGAIDLMLPGYLVYSTYVNGGYASSSGTSMASPHGAGLAALH
                     VARYGRAANASGVAAVRQALIDAGRDQTGTSGLT"
BASE COUNT          298 a          732 c          728 g          259 t
ORIGIN      
        1 ttctttacct tcaccatggc gcgcttgccg ggctgataca cgaggtcgcg gtgcttcgcg
       61 accacgccgt cgacgcctcc gccgcagaag cgctcgagcc acccttcggc gaccttcgga
      121 tcgtccgtga cgggggtcac gacgaggggt ccgtcggccg gcgcggcgag cgcctcgagc
      181 gcggcgcgcc gctcgacgaa gggcgccgcg cggaggtcct cgcccgccgc cgcgaggagg
      241 tcgaaggcaa cgaagcgggc gggagtctcg cgccgtagcc gctcgacgcg cgacgcggca
      301 gggtgcaggc gggccatgat cgcggcgaag tcgaacgcgc cgtctgtgag tacgacgatc
      361 tcgccgtcga gcgccacggc gtcgtcgcga agcgagcgga tcccggccac gagctcgggg
      421 aagtagcgcg cgagcggacg gtcgttgcgg cttcggatgt cgaccgcgtc gccgtcgcgg
      481 aaggcgaggg cgcggaaccc gtcccacttg ggctcgtaca ggtagcggtc gtggggcagc
      541 tcgcgctcga gccgggcgag catcggcgga agcggcgggc ggaccggaag ccgcacgaca
      601 gcttcgtacc acgcccgcgg gatccgcgca ccgatgcgcg ccccgcgcgc cccaggagac
      661 cccccaaccg gggggtctac agcctcgtga cctgcggtac catccgccgg ccgtcgcacg
      721 tcttcacgag gaggggtcca tgaaacgcgc tgttctgtcg ctcgtggtcg cactggccgc
      781 ggccgcgctc ctgtcgccag ccgcagccgc cggcaaggca ggccgctaca tcgtcgtgct
      841 ccgcgcttcc gcggacgcgc cgaccgtcgc ggcccagcat gcgcgcagcc acggcgccgc
      901 cgtgtcgcac gtctacgagc acgcgctgcg cgggtacgcc gcgcgcctgg gcggcgccgc
      961 cgtcgcgagg atcgagcgcg acgcccgcgt cgcgtacgtc gagcccgatc tccgcgtcca
     1021 tgcgtttgcg caggttctac cgacgggcgt cgaccggatc gacggcgagc tctcccccac
     1081 ggcgaagatc gacggcctcg acgacgacgc ggtcggctcg gtcgagcgtg tgaacgtcga
     1141 cgtcgcggtc atcgacacgg gcgtccagtt caaccatccc gacctgaacg tcgtcgcgat
     1201 gacggactgc acgggcggcg ggccgttcag gcagaagtgc accgacggaa agggcgagga
     1261 ccagaacggc cacgggacgc acgtcgcagg cacgatcggc gcgctcgaca acggcatcgg
     1321 cgtcgtcggc gtcgcgcctg gcgcccgcat ccacaacgtc cgcgtgctcg actcgcgcgg
     1381 cagcggctac acgtcgtgga tcatcgccgg gatcgactgg gtgaccgcgc gcgcgtccac
     1441 gatcgaggtc gcgaacatga gcctgggcgg cagcggctac acggcggcgc agcacaccgc
     1501 ggtcaagaac tcggtcgcga agggggtcgt ccacgtcgtc gcggccggga actcggcgcg
     1561 tgacgtctac ggcccggacg ggaggttcgg aagctccgac gactcccagc cggcctcgta
     1621 tccggaggcg atgacgatct ccgcgctcgc ggacgcggac ggtagatcgg gcgggctcgc
     1681 cgccacgagc acgaccttct cgatctgcac ggagaaccgc gacgactcct tcgcgtgctt
     1741 ctcgaacttc agccggagcg tggtcgcggg gaatcccgtc gtctcgaccg gcggcgcgat
     1801 cgacctcatg ctccccgggt acctcgtcta ctcgacgtac gtgaacggcg gatacgcctc
     1861 gtcgagcggg acgagcatgg cctcgccgca cggcgccgga ctggccgcgc tccacgtcgc
     1921 ccggtacggc cgcgccgcga acgcaagcgg cgtcgccgcc gtccgccagg cgctcatcga
     1981 cgcaggccgc gaccagacgg gcacgagcgg cctcacc
//
LOCUS       DASZOB010000058         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_30879, whole genome shotgun sequence.
ACCESSION   DASZOB010000058 DASZOB010000000
VERSION     DASZOB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30879"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VG709_00935"
     CDS             complement(<1..495)
                     /locus_tag="VG709_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWC31674.1"
                     /translation="MGGPSPDPAQARVLDHERGSLLVTGPPGSGKTWLLRERFCRLVE
                     AGVSPERVALVTLNRRATRDARDWIVHRLARSVPEIQVFTVHGFAFRVVGRRFDEIDG
                     FEEPPDILSAADQYAVVRQMLRAERREDWPELGGLLDSRGFAQHVADLVLRAQERLLL
                     PDDLD"
     gene            complement(538..1287)
                     /locus_tag="VG709_00940"
     CDS             complement(538..1287)
                     /locus_tag="VG709_00940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC31675.1"
                     /translation="MSVHEAAARGFGAAAEAYDRARPDYPDQAIDRLIQELEINRAAT
                     LLDLAAGTGKLTRQLIPTGARVVAVEPVEAMRARLPQELGHLEVVAAVAEALPFGGDS
                     FDGVLVAQAFHWFDGARALGEIHRVLKPSGRLALLWNVRDESVEWVRRLGEIFDRYER
                     EVPREKTGEWRRAFTVTDYFGTLHQLRFQHAQRLDRDGLVERVASTSFVASLAEGERD
                     EVLQQVRGLADEVGDEIVLPYVTELYWTSKI"
     gene            complement(1284..>1631)
                     /locus_tag="VG709_00945"
     CDS             complement(1284..>1631)
                     /locus_tag="VG709_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31676.1"
                     /translation="GGGAEWDGRRAVVRADDDDVRATLQRVFRVSPVVTPDPAARPTG
                     SRADAIVEPGTLEWFREAAAVRGEAEGLSARFVTTRPGGWDPAGSYVPMELWVGRREG
                     DVGRLPSLGTSRA"
BASE COUNT          239 a          614 c          572 g          206 t
ORIGIN      
        1 gtcgaggtcg tccggcaaca gcagccgttc ctgcgcgcgg agcacgaggt cggcgacgtg
       61 ctgcgcgaag ccgcgcgaat cgaggaggcc tccgagctcc ggccagtcct cgcgccgctc
      121 ggcccggagc atctgccgca cgacggcgta ctggtcggcg gccgagagga tgtccggcgg
      181 ctcttcgaac ccgtcgatct cgtcgaaacg gcgcccgacg acgcggaacg cgaacccatg
      241 gactgtgaag acctggatct cgggtaccga gcgggcgagg cggtggacga tccagtcgcg
      301 ggcgtcccgc gtggcccggc ggttcagcgt gacgagggcg acccgctctg gcgagacgcc
      361 cgcctcgacg agccggcaga accgctcacg gaggagccac gtcttgccgg agccgggagg
      421 gcccgttacc agcaaggacc cgcgctcgtg gtcgagcacg cgagcttgcg ccggatcggg
      481 ggaggggccg cccatgcctg gatgctatcc cgtgccactg acacgccttc gcggcgctca
      541 gatcttcgag gtccagtaca gctcggtgac gtacggcagc acgatctcgt cgcccacctc
      601 gtccgccagg ccgcggacct gctggagaac ctcgtcgcgc tccccctcgg ccaacgacgc
      661 gacgaagctc gtcgatgcca cgcgctccac gagcccgtcg cggtcgaggc gctgtgcgtg
      721 ctggaaccgc agctggtgga gcgtcccgaa gtagtcggtc acggtgaacg cgcggcgcca
      781 ctcgccggtc ttctctcgcg ggacctcgcg ctcgtaccga tcgaagatct cgccgagccg
      841 gcgcacccac tcgacggact cgtcgcggac gttccacagc aacgcgagac gccccgaggg
      901 cttcagcacg cggtggatct ccccaagcgc gcgcgcaccg tcgaaccagt ggaacgcctg
      961 cgcgaccagg acgccgtcga acgagtcgcc tccgaacggc agcgcctccg caaccgccgc
     1021 gacgacctca aggtgcccga gctcctgagg cagccttgcg cgcatcgcct cgaccggctc
     1081 gacggcgacg acgcgggcgc ccgtcggtat cagctgccgc gtcagcttcc cggttcccgc
     1141 cgcgaggtcg aggagcgtcg cggcgcggtt gatctccaac tcttggatca gccgatcgat
     1201 cgcctggtcg gggtagtcgg gacgcgcccg gtcgtacgcc tcggcggccg cgccgaagcc
     1261 gcgggccgcc gcctcgtgga cgctcacgcc cgggacgtgc cgagggaggg gagtctcccg
     1321 acgtccccct cgcgccggcc gacccacagc tccataggga cgtaagagcc cgcggggtcc
     1381 cagccgcccg gccgcgtcgt cacgaaccgt gccgacagcc cctcggcctc gccgcggacc
     1441 gccgccgcct cacggaacca ctcgagggtg ccgggctcga cgatcgcatc ggcgcgcgag
     1501 ccggtcgggc gcgcggccgg atcgggcgtc acgaccggcg agacgcggaa gacgcgctgg
     1561 agcgtcgcgc ggacgtcgtc gtcgtcagcg cgaacgaccg ctcggcgccc gtcccactcg
     1621 gcgccgccgc c
//
LOCUS       DASZOB010000059         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_152696, whole genome shotgun sequence.
ACCESSION   DASZOB010000059 DASZOB010000000
VERSION     DASZOB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152696"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VG709_00950"
     CDS             <1..383
                     /locus_tag="VG709_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC31677.1"
                     /translation="EGLPLTAFWAPLLVLLEIPLVAGVVLLGSAMNVFARDIRIIVPL
                     FVQFWLLLTPVMYALRDVPQHLRGFYLANPMTGLVESFRDVLIRGVAPSLERLLPTMI
                     GAAVLFVVGVWYFRSTEPRFADVI"
     gene            385..915
                     /locus_tag="VG709_00955"
     CDS             385..915
                     /locus_tag="VG709_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC31678.1"
                     /translation="MNALEIDDITKTYRIGVGRARIREALPWPLDAAFARLFPRWWSK
                     NTFNALEDVSFTVPKGASVGIVGHNGAGKTTMLKVIAGVTAPSRGSITANGRLGALID
                     VLVGFHPDLTGAENAYLLGAIHGLSRKEMSQRIHRILEFAEIDDLAETPVKRYSSACR
                     RAWASRRSSRSSPRSC"
     gene            858..1607
                     /locus_tag="VG709_00960"
     CDS             858..1607
                     /locus_tag="VG709_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31679.1"
                     /translation="MQARLGFSTLVAIEPEILLIDEVLAVGDANFQRKCTEWLDEYRR
                     RGGTLLFVSHNLGLVRSMTDRVLWLDHGKVVEEGETGDVLVRYARAMERRDVGDTDRR
                     TKGARKVIAARGLYRWGAGGARVQEVRIDGGQDEGRGVDVTITYESTSLERAVFCVAF
                     VDEGGQEIAAAVSPEISVSREGAVRCRIDPMPFREGIYFPVAAILAPSGQVEDRWRLE
                     RAIVVNADGDVAMPPDFGAVRLDAIWKEGAE"
     gene            1618..2451
                     /locus_tag="VG709_00965"
     CDS             1618..2451
                     /locus_tag="VG709_00965"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWC31680.1"
                     /translation="MSGLPTFLIVGAMRSGTTSLARYLAQPPDVFFSPKKEIHFFDFN
                     HEKGTDWYRSFFDGADAARAAGEATPSYMYVAEAVPRMAALVPDARLVAILRDPVERA
                     YSHYWHERALGRERLGFAEAIAAEPERLRSPDPVARARAAYLDRGRYAQQLERLAEHY
                     RRDAMHVLLLEDLQKEPRATYAELCRFLGIDDGFLPEGLTERHNVSRSLRSLTLRRVT
                     GRLPRDSRFGAWLRRVNRRGQGYPAMDPDVRARLREEFRGPNADLARWLGRDVSAWDE
                     A"
     gene            2448..3287
                     /locus_tag="VG709_00970"
     CDS             2448..3287
                     /locus_tag="VG709_00970"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWC31681.1"
                     /translation="MSDRGGAHGRLPTFLVVGAQKSGTTSLVHYLGAHPDVFALPDEV
                     HFFDRNFERGTDWYREHFRAAGRERHVGESTPEYMYAQEAPRRIADTLGDVRLLAILR
                     DPVDRAYSAYWHNRTRGHEQLAFEEALDAEQCRLASGDESVAMRFAYVDRGRYAAQLR
                     RVREALPEAPLSVTLFERLRDERVETVRSLYRFLGVDDAVVPPQIAEVKNRFVEFRSQ
                     RLRRPIRRLPGPLRRIAGRLNIRYRTYPSLSAELRARVAERFRDDNVELASWLGLDLS
                     PWT"
     gene            complement(3271..4230)
                     /locus_tag="VG709_00975"
     CDS             complement(3271..4230)
                     /locus_tag="VG709_00975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC31682.1"
                     /translation="MNEHFIRRDRCPACGAGEHVELYRCAYTSPPISAFLVRTYGRAG
                     HIDLEYLQGADYALRECGACGLIFQHEVPNDHLMRILYEEWVSAEFSLHKRARNAAAV
                     RGRIALEIMAIADLLQRPPESLRVLDFGMGWGDWCRMAQAFGCEAFGVETASSRIEHA
                     RKLNVPVLAPGDLPPASFDVVNSDQVLEHLAEPYGAVAVVAEALRPHGLLKISVPNGR
                     RVKRRLRRPDFSAPKGSRRSLHDVSPLQHVNCFQHASLVRLVERAGLRRIPFPATLRY
                     RYAPTWERPANLLRRTVGQHVRASLRQETVVWFRKSGTLTSTG"
     gene            4269..>4529
                     /locus_tag="VG709_00980"
     CDS             4269..>4529
                     /locus_tag="VG709_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31683.1"
                     /translation="MSLRILHVVTTPIRRGAEMFAADLVAALGGDVEQRVLLLRRGAG
                     PNPRASVRSAASVRRFTRSGGAATRPAGRPAFGAANSSSRPAP"
BASE COUNT          634 a         1603 c         1617 g          675 t
ORIGIN      
        1 gcgaaggact gcccctcacc gcgttctggg cgccgctgct cgtgctcctg gagatcccgc
       61 tcgtcgcggg cgtcgtgctc ctcgggagcg cgatgaacgt gttcgcccgc gacatccgga
      121 tcatcgtgcc gctcttcgtg cagttctggc tgttgctcac gccggtcatg tacgcgctcc
      181 gagacgttcc ccagcacctc cgtggcttct acctcgcgaa cccgatgacg ggcctcgtcg
      241 agtcgttccg cgacgtgctg atccgcggcg tcgcgccgag cctcgaacgg ctgctgccga
      301 cgatgatcgg cgccgccgtg ctgttcgtgg tgggcgtctg gtacttccgc tcgaccgagc
      361 cgcggttcgc ggacgtgatc tgacgtgaac gcactcgaga tcgacgacat caccaagacg
      421 taccggatcg gcgtcggccg ggcgcggatc cgggaggctc taccgtggcc gctcgacgcg
      481 gcgttcgcgc gcctcttccc gcggtggtgg tcgaagaaca cgttcaacgc gctcgaagac
      541 gtctcgttca cggtcccgaa gggcgcctcg gtcgggatcg tcggccacaa cggcgccggc
      601 aagaccacga tgctgaaggt catcgcgggc gtgacggcgc cgagtagagg gtcgatcacg
      661 gcgaacggca ggctcggcgc gctgatcgac gtcctcgtcg ggttccaccc cgacctcacc
      721 ggcgcggaga acgcgtacct gctcggagcg atccacggtc tgtcccgcaa ggagatgtcg
      781 cagcgcatcc accgcatcct cgagttcgcg gagatcgacg acctcgccga gacgcccgtc
      841 aagcggtact cctcggcatg caggcgcgcc tgggcttctc gacgctcgtc gcgatcgagc
      901 ccgagatcct gctgatcgac gaggttctcg ccgtcggcga cgcgaacttc cagcgcaagt
      961 gcaccgagtg gctcgacgaa taccgccgtc gcggcggcac gctgctcttc gtctcccaca
     1021 acctcgggct ggtccggagc atgaccgacc gggtcctgtg gctcgaccac ggcaaggtcg
     1081 tggaggaggg cgagaccggc gacgtgctcg tgcgctacgc gcgcgcgatg gagcggcgcg
     1141 acgtcggcga caccgaccgc cggacgaagg gcgcccggaa ggtcatcgca gctcgtggcc
     1201 tctaccgctg gggcgccgga ggcgcccgcg tccaggaggt ccgcatcgac ggcggacagg
     1261 atgagggccg cggcgtcgac gtcacgatca cgtacgagtc cacgtccctc gagcgcgcgg
     1321 tgttctgcgt cgcgttcgtc gatgagggcg gccaggagat cgccgccgcg gtctcgccgg
     1381 agatctccgt gtcgcgcgag ggcgcggtgc ggtgccggat cgacccgatg ccgttccgcg
     1441 aggggatcta cttcccggtc gcagcgatcc tcgcgcccag cggacaggtc gaggaccggt
     1501 ggcggctcga gcgcgcgatc gtcgtgaacg ccgacggcga cgtcgcgatg ccacccgact
     1561 tcggcgctgt gaggctcgac gcgatctgga aggaaggcgc ggagtagctc ggctcgcatg
     1621 agcggtcttc cgacgttcct gatcgtcggc gcgatgcgct cggggacgac gtcgctcgcg
     1681 cgctacctcg cgcagcctcc cgacgtcttc ttctcgccga agaaggagat ccacttcttc
     1741 gacttcaacc acgagaaggg gacggactgg taccgctcgt tcttcgacgg agccgacgcc
     1801 gcgcgtgcgg ccggggaggc gacgccgtcc tacatgtacg tcgcggaggc cgtgccgagg
     1861 atggcggcgc tcgtgcccga tgcgaggctc gtcgcgatcc tgcgcgatcc cgtcgagcgc
     1921 gcgtactccc attactggca cgaacgagcg ctcgggcgcg aacggctcgg gttcgccgag
     1981 gcgatcgccg cggagccaga gcggctgcgg tcgccggacc ccgtcgcccg cgcccgcgcc
     2041 gcctatctcg accgcggccg gtacgcgcag cagctcgagc gcctcgccga gcactaccgg
     2101 cgcgacgcga tgcacgtgct gctgctcgag gacctgcaga aggagcctcg agcgacgtac
     2161 gcggagctct gtcggttcct cgggatcgac gacgggttcc tccccgaggg cctcaccgag
     2221 cggcacaacg tcagccgctc gctccggtcg ctgacgctgc gacgcgtcac cgggcggctg
     2281 ccgcgcgaca gccgcttcgg ggcgtggctc cggcgggtca accgccgcgg gcagggctat
     2341 cccgcgatgg atccggatgt ccgtgcccgg ctgcgcgagg agttccgcgg cccgaacgct
     2401 gacctcgcgc gttggctcgg tcgcgacgtg tccgcgtggg acgaggcgtg agcgaccgag
     2461 ggggcgccca cgggcgcctt ccgacgttcc tcgtcgtcgg cgcgcagaag tcggggacga
     2521 cgtcgctcgt gcactacctc ggggcgcatc ccgacgtgtt cgcgctcccc gatgaggtgc
     2581 acttcttcga ccgcaacttc gagcgcggga ccgactggta ccgcgagcac ttccgcgcgg
     2641 cggggcggga gcggcacgtc ggtgaatcca cgcccgagta catgtacgcg caggaggcgc
     2701 cccgccggat cgccgacacc ctaggagacg tccggctgct tgcgatcctc cgcgacccgg
     2761 tcgaccgggc gtattcggcc tactggcaca accgcacgcg gggccacgaa cagctggcgt
     2821 tcgaagaggc gctcgacgcg gagcagtgcc ggctcgcgtc cggcgacgag tccgtcgcga
     2881 tgcggttcgc atacgtcgac cgcggccgct acgctgcgca gctgcgtcgc gtgcgcgagg
     2941 cgctcccgga agcgccgctc tccgtcacgc tcttcgagcg gctgcgggac gaacgcgtcg
     3001 agaccgtgcg ctcgctctac cgcttcctcg gcgtcgacga cgcggtcgtg ccgccgcaga
     3061 tcgcggaggt gaagaaccgc ttcgtcgagt tccggtcaca gcgcctccgc cgcccgatcc
     3121 gccgcctgcc cggacccctc cgccgtatcg ccggtcgtct caacatccga tacaggacgt
     3181 acccgtcgct ctcggcggag ctccgcgccc gcgtcgccga gcgcttccgt gacgacaacg
     3241 tggagctcgc gtcgtggttg ggactcgacc tcagcccgtg gacgtgaggg ttccggactt
     3301 ccggaaccag acgacggttt cctgccggag cgaggcgcgc acgtgctgcc cgaccgtgcg
     3361 ccggaggagg ttcgccggac gctcccaggt cggcgcgtag cggtagcgga gcgtcgcggg
     3421 gaacggaatc cgccggagcc cggcgcgctc gacgagccgg acgagcgacg cgtgctggaa
     3481 gcagttcacg tgctgcagcg ggctcacgtc gtggagcgac cgccgcgaac ccttcggcgc
     3541 cgagaagtcg gggcgccgca gccgccgctt cacccgccgg ccgttcggca cgctgatctt
     3601 gaggagcccg tgcgggcgaa gcgcctcggc gacgaccgcg acggcgccgt acggctcggc
     3661 gaggtgctcg aggacctggt cgctgttgac gacgtcgaac gaggccggag ggaggtcgcc
     3721 cggggcgagg accggcacgt tgagctttcg cgcgtgctcg atccgcgacg aggccgtctc
     3781 gacgccgaac gcctcgcacc cgaacgcctg cgccatgcgg caccagtcgc cccaccccat
     3841 cccgaagtcg aggacgcgga ggctctccgg cgggcgttgc aggaggtccg caatcgccat
     3901 gatctcgagc gcgatccgcc cgcgcacggc ggccgcgttc ctggccctct tgtggaggga
     3961 gaattcggcg ctcacccact cctcgtagag gatccgcatc aggtggtcgt tggggacctc
     4021 gtgctggaag atcagcccgc acgccccgca ctcgcggagg gcgtagtccg ccccctgcag
     4081 gtactcgagg tcgatgtggc cggcgcgacc gtacgtccgc acgaggaagg cgctgatcgg
     4141 cggactcgtg tacgcgcagc ggtacagctc cacgtgctcg ccggcgccgc aagccggaca
     4201 acggtcacgg cggatgaagt gctcgttcac ggcggcgagt gtaggggact agcggtaacg
     4261 ttcggccgat gtcgctgcgg atactgcacg tcgtcacgac gccgattcgt cgcggcgccg
     4321 agatgttcgc ggccgacctc gtcgccgcgc tgggcggcga cgtcgagcag cgcgtgctgc
     4381 tgctgcgccg gggggcgggg ccgaacccgc gcgcctcggt acgcagcgcc gcgagcgtcc
     4441 ggaggttcac gcgcagcggc ggcgctgcga cgcggccggc cggaaggccg gcgttcgggg
     4501 ccgcgaactc gagctcccgc cccgccccc
//
LOCUS       DASZOB010000060         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_61923, whole genome shotgun sequence.
ACCESSION   DASZOB010000060 DASZOB010000000
VERSION     DASZOB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61923"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VG709_00985"
     CDS             complement(<1..679)
                     /locus_tag="VG709_00985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC31684.1"
                     /translation="MAQPPEPARRVDAKQATVLYHDAAAAAYDEKWAITFEEPAVRYV
                     RDRADRMLRRPRYDRVLEIGAGTGFFILNLWQAGFVGEAHATDISPRMLDMSRRNAAR
                     VGCSLEVRIADAERLPYDDAAFDLVVGHAFLHHIPDPVAALREIHRVLRPGGALFIAG
                     EPTRIGDRLASVPKAAVRTAFRTAARLPLLRRLARDGTPLPPPATEEDRILRALEWDG
                     ALHTVSPG"
     gene            complement(682..1455)
                     /locus_tag="VG709_00990"
     CDS             complement(682..1455)
                     /locus_tag="VG709_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWC31685.1"
                     /translation="MGDLVRLEVEGGVGTIRLDRPPMNAINEELTRDLLEVATEAGGR
                     DDVGAVVLYGGEKVFAAGADIKAMADAGTAEIRPYIQGLQEVFNAVEDIPKVTVAAVT
                     GFALGGGCELALCADFRVAAEDAQLGQPEILLGIIPGAGGTQRLPRLVGPARAKDLIY
                     SGRRITAIEAEDIGLVDVVAPPGGAYDEAMKIAKRYAGGPRAALRAAKIAINWGGRTD
                     LRTGLTIERETFTDLFATEDQKEGMRAFLEKREAAFRGR"
     gene            complement(1469..1897)
                     /locus_tag="VG709_00995"
     CDS             complement(1469..1897)
                     /locus_tag="VG709_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWC31686.1"
                     /translation="MATFDERTRELLSEVPLFEGLSRKELESILQTAKDVNVDAGKKI
                     VEQGDNGVGFHLILEGACKVTVHDHEVATLGRGDYFGEMSLIDGDPRSATVVAETPVR
                     TLSLTSWTFLPLLEQNPSVARKLLVEMSRRLRVAERSIKH"
     gene            1960..2844
                     /locus_tag="VG709_01000"
     CDS             1960..2844
                     /locus_tag="VG709_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC31687.1"
                     /translation="MDVMGDWSGEFPFEPRFAEVTQDVRLHYVEEGDGEPVLMLHGNP
                     TWSFLYRKIIPAVAAAGFRAVAVDHMGFGRSDRPSGHERYTMRVHVENLLGFIRALDL
                     RGITLVMQDWGGPIGFGAAVEEPDRIARLVILNTGVGVFPSGTPMPFHEPFRTRGLGE
                     MLALGANLFVEAMFGGMRPESATPLVAEAYRAPFPDYYSRVPILAFTRDVPIGHEHPT
                     APYMKEVADRLPSLERPTLVVWGMRDRTFPPMLLDVWRARYPHAEVLELPEARHYLQE
                     DEPEAISTAIVEFLRRAG"
     gene            2887..3309
                     /locus_tag="VG709_01005"
     CDS             2887..3309
                     /locus_tag="VG709_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWC31688.1"
                     /translation="MDEAASVVAMLREHGYRITPQRQAIVREVLRARGHVVPQDVARR
                     VQRRLPGVNASTVYRTLATLEEVGLLRHAHLERGAEYHRTGDVEHVHLTCSRCGAEED
                     LGIREAGSLKRLLERSRGFTPDLTHFAISGVCARCRSA"
BASE COUNT          492 a         1220 c         1173 g          510 t
ORIGIN      
        1 cccccggcga gaccgtatgg agggcgccgt cccactcgag cgcgcgaagg atccggtcct
       61 cctcggtggc cggcggaggg agcggggtcc cgtcccgcgc caggcgccgt agcagtggga
      121 gccgggcggc cgtgcggaac gccgtccgga ccgcggcctt cggcaccgag gcgagacggt
      181 cgccgatccg cgtcggctcc cccgcgatga agagcgcccc gcccggccgg agcacgcgat
      241 ggatctcgcg gagggccgcg accggatccg ggatgtggtg caggaaagcg tgcccgacga
      301 cgaggtcgaa cgccgcgtcg tcgtacggga gacgctcggc gtcggcgatg cgcacctcga
      361 gcgagcaccc gacgcgcgcc gcgttccgcc gagacatgtc gagcatccgc ggcgagatgt
      421 cggtcgcgtg tgcctcgccg acgaacccgg cctgccacag gttcaggatg aagaagcccg
      481 tccccgcgcc gatctcgagg acgcggtcgt agcgcgggcg acggagcatc cggtcggcgc
      541 ggtcccgcac gtaacgcacc gcgggctcct cgaaggtgat cgcccacttc tcgtcgtacg
      601 ccgccgcggc ggcgtcgtgg tagaggacgg tggcctgctt cgcgtccact cgccgcgcgg
      661 gctccggcgg ctgcgccacc gctaccgtcc gcggaacgcg gcttcccgct tctccaggaa
      721 ggcgcgcatc ccctctttct ggtcctcggt cgcgaagagg tccgtgaacg tctcgcgctc
      781 gatcgtgaga ccggtacgca gatcggtgcg gccgccccag ttgatcgcga tcttcgcggc
      841 ccgcaacgcg gcgcgcgggc cgccggcgta gcgcttcgcg atcttcatgg cctcgtcgta
      901 cgcaccgccc ggtggggcca cgacgtcgac gagcccgatg tcctccgcct ctatcgcggt
      961 gatgcggcga cccgaataga tgaggtcctt ggcgcgcgcc ggaccgacga ggcgcgggag
     1021 gcgctgcgtc cctccggcgc cggggatgat ccccagcagg atctcgggtt ggccgagctg
     1081 cgcgtcctct gccgcgacgc ggaagtccgc gcagagcgcc agctcgcagc cgccgccgag
     1141 cgcgaacccc gtcaccgccg cgaccgtcac cttcgggatg tcctcgaccg cgttgaagac
     1201 ttcctggagt ccctggatgt acggccggat ctcggcggtt ccggcatcgg ccatcgcctt
     1261 gatgtccgcg cccgctgcga acaccttttc gccgccgtag agcacgacgg cgccgacgtc
     1321 gtcgcgcccg ccggcctccg tggcgacctc gaggaggtct cgggtcagct cctcgttgat
     1381 cgcgttcatc ggcggccggt cgagacggat ggtccccacg ccgccctcga cctcgagacg
     1441 gaccaggtct cccatggcag gcctccagtc agtgcttgat cgagcgttcg gcgacacgca
     1501 gccgtcggct catttcaacc agcagcttgc gtgcgacgga cggattctgc tcgaggagcg
     1561 gcaggaaggt ccacgacgtc agcgaaaggg tccgcaccgg cgtctccgcc acgaccgtcg
     1621 ccgaacgagg atcgccgtcg atgagcgaca tctccccgaa gtagtcgccg cggcccagcg
     1681 tcgcgacctc gtggtcatgc accgtcacct tgcacgcgcc ctcgaggatc aggtggaacc
     1741 cgacaccgtt atcaccttgc tcgacgatct tcttccccgc gtcgacgttc acgtccttgg
     1801 cggtctgcag gatcgactcg agctccttcc gcgacaaccc ctcgaagagc ggtacctccg
     1861 aaaggagctc acgcgtgcgc tcgtcgaagg tggccatcga tcctcccgtt cgggacgcgg
     1921 gcatcataga ggagcggccg gggctacgct gtggcgccta tggacgtcat gggcgactgg
     1981 agcggcgagt tcccgttcga gccgcggttc gccgaggtca cgcaggacgt tcgtctgcac
     2041 tacgtcgagg agggcgacgg cgagcccgtg ctcatgctcc acggcaaccc gacgtggagc
     2101 ttcctctacc ggaagatcat cccggccgtc gccgcggcgg gcttccgcgc cgtggcggtc
     2161 gaccacatgg gcttcggccg gtcggatcgc ccgagcggtc acgagcgcta cacgatgcgc
     2221 gtccacgtgg agaacctcct cgggttcatc cgcgcgctcg acctccgcgg catcacgctc
     2281 gtcatgcagg actggggcgg gccgatcggg ttcggcgccg cagtcgagga acccgaccgg
     2341 atcgcgaggc tcgtgatcct gaacaccggc gtcggcgtct tcccgtcggg gaccccgatg
     2401 ccgttccacg agccgttccg gacccgcggg ctgggcgaga tgcttgcgct cggagcgaac
     2461 ctgttcgtcg aggcgatgtt cggtgggatg cggcccgaga gcgcgacgcc gctcgtcgcg
     2521 gaggcgtacc gagcgccgtt ccccgactac tactcgcgcg tgccgatcct ggcgttcacg
     2581 cgcgacgtgc cgatcggcca cgagcacccg acggcgccgt acatgaagga ggtcgccgat
     2641 cggctccctt cgctcgagcg gccgacgctc gtcgtgtggg ggatgcgtga tcgcacgttc
     2701 ccgcccatgt tgctcgacgt ctggcgcgcg aggtacccgc acgccgaggt gctggagctg
     2761 ccggaggcgc gtcactatct ccaagaggac gagcccgagg cgatctcgac ggcgatcgtc
     2821 gagttcctcc gccgcgccgg ctagacggcc gccgcacctg caacgcggtt gcagtaccgt
     2881 tcccgcatgg acgaggcggc ttcggtcgtc gctatgctgc gcgagcacgg ataccgcatc
     2941 accccgcaac ggcaggcgat cgtgcgcgag gtcctgcgcg cgcgaggcca cgtcgttccg
     3001 caggacgtcg cgcgacgcgt ccagcggcgc ctccccgggg tcaacgcctc gaccgtgtac
     3061 cggacgctcg cgacgctgga ggaggtcggc ctgcttcgcc acgcacacct cgagcggggc
     3121 gccgagtacc accgcacggg cgacgtagag cacgtccacc tgacgtgctc ccgctgcggc
     3181 gcagaggagg atctcgggat ccgcgaggca gggtcgctca agcggttgct ggaacgctct
     3241 cgcggcttca cgcccgacct cacgcacttc gcgatctccg gggtctgcgc ccgctgccgg
     3301 agcgcttaac cggcaaagag cgccggcaac aacccctcga gcccgaggag gaagagcgtc
     3361 ccgaggacga ggctgaacac cgccgtgatg acggc
//
LOCUS       DASZOB010000061         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_172215, whole genome shotgun sequence.
ACCESSION   DASZOB010000061 DASZOB010000000
VERSION     DASZOB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172215"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="hppD"
                     /locus_tag="VG709_01010"
     CDS             complement(<1..867)
                     /gene="hppD"
                     /locus_tag="VG709_01010"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HWC31689.1"
                     /translation="MANQEDFLPLMGIDHVEFYVGNARQAAYYYSKAFGFGITAYAGP
                     ETKVRDRASWVCEQGDVRYVFTGGMAPESDVVKHVHEHGDGVKDVALRVPDATDAWEQ
                     TTKRGAESVMEPEIFEDEHGKVVRAAIKAYGDTIHSFIERENYSGPFLPGYRAEEAHP
                     DDGPPVGIRAIDHVVANVELGKMNEMVEYYARLLGFAQLVHFRDDQISTEYTALMSKV
                     MWDGKGRVKFPINEPAEGKKKSQIQEYLEFYRSPGVQHIAMAVDDIITAVDALRQRGV
                     RFIRVPKTYYEDL"
     gene            complement(918..3557)
                     /locus_tag="VG709_01015"
     CDS             complement(918..3557)
                     /locus_tag="VG709_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC31690.1"
                     /translation="MPTDTLPTGTVTFLFSDIEGSTKLLNALGEDYPPLLETHQRLMR
                     DAFERHGGLEIATEGDSFFVAFRSAPDAVAAAVAAQRALAVHDWGDAGPVRARIGLHT
                     GEGLLGGDNYVGIDVHRAARIMSAGHGGQIVVSEPTRALVRQTLPEGATIRDLGEHRL
                     KDLPEPEHLFQVDVEGLPAEFPPLRTLERRVANLPEQLTTFVGRRRELDDVISILSKT
                     RLLTLTGPGGTGKTRLSIEVARETVDDFDDGAAFVALSPLRDPALVVPTIAQALGLRE
                     DPSRPPVDAVAENLRDKRMLVVLDNFEQVMEAASDIGQLLAVTSGATFLVTSREALGL
                     HGEREYQVPPLALPDVRRLPPLDALSQYEAVRLFIDRATAVKTGFSITNENAPAVAEI
                     TSRLDGLPLAIELAAARIKLLSPQAILSRLEERLSLLVGGARDRPARQQTLRDAIAWS
                     YDLLDEPERRFFSRLAIFRGGFTLEAAEEVCNPDAELGLDTFDGIESLVNKSLLRQTE
                     VPSGEPRFFMLFTIRDYAGERLAEGREQADIARRHQEFFLGLAERARPELLGPRQADW
                     LDALEREHDNIRAVLDRASDIEFRLRLSAALWRFWQFRGHLREARTRLEELVARPEAR
                     EQPRALAEALEGAGGVAYWMGDWAACALHYGEMLTLYRELGDKRGEAEALYNLSFPYH
                     VPVTGGQEDLDRARDLLEGSLRLFGELGDQAGLAKVTWGLSNLYYRLGDLDRAERAAE
                     ESLEAYRRLDDRFGAGWALHSLGVSKMRRGDLDGAESRFREALRIFADARDSSGLTLV
                     LADCAVLAWERGQRPRGARLAGAAFALQEASGTDLALFVADEEQFQEDLRQARESADT
                     ADDLAAGRRMSVDEAVNLALEAS"
BASE COUNT          522 a         1284 c         1220 g          567 t
ORIGIN      
        1 gagatcctcg tagtacgtct tgggcacgcg gatgaaccgc accccgcgct gccggagcgc
       61 gtcgacggcc gtgatgatgt cgtcgaccgc catcgcgatg tgctgcaccc cgggcgaccg
      121 gtagaactcg aggtactcct gaatctgcga cttcttcttg ccctcggccg gctcgttgat
      181 cgggaacttg acgcgtccct tgccgtccca catgaccttc gacatgagcg cggtgtactc
      241 ggtcgagatc tggtcgtcgc ggaagtgcac gagctgcgcg aagcccagca gccgcgcgta
      301 gtactcgacc atctcgttca tcttcccgag ctcgacgttg gccacgacgt ggtcgatggc
      361 cctgatgccg accggcgggc cgtcgtccgg atgcgcctcc tcggcgcgat agccggggag
      421 gaacggcccg gagtagttct cgcgctcgat gaacgagtgg atcgtgtcgc cgtaggcctt
      481 gatcgcggca cgcacgacct tgccgtgctc gtcctcgaag atctccggct ccatgacgct
      541 ctcggccccg cgcttcgtcg tctgctccca cgcgtcggtc gcgtcgggaa cgcggagcgc
      601 gacgtccttg acgccgtctc cgtgctcgtg gacgtgcttc acgacgtcgc tctccggcgc
      661 catcccgccg gtgaagacgt agcggacgtc gccctgctcg cacacccacg acgcgcggtc
      721 gcgcaccttg gtctcgggac cggcataggc ggtgatcccg aacccgaacg ccttcgagta
      781 gtagtaggcg gcctgtcgcg cgttcccgac gtagaactcg acgtggtcga tccccatcag
      841 gggaaggaag tcctcctggt tagccatcgc cctcacctcc tacggctcga ctatacccat
      901 ggccgcctcg gcggccgcta cgacgcttcg agcgcaaggt tgacggcctc gtcgaccgac
      961 atccgccggc cggcggctaa gtcgtcggcg gtatccgcgc tctctcgtgc ctgccgcagg
     1021 tcctcctgga actgctcctc gtccgcgacg aagagcgcga ggtcggtccc actcgcctcc
     1081 tggagcgcga acgccgcgcc ggcgagccga gcgccgcggg ggcgctgtcc cctctcccac
     1141 gccaggaccg cgcagtccgc caggaccagc gtgagcccgg aggagtcgcg ggcgtcggcg
     1201 aagatcctga gggcctcgcg gaaccgggac tcggcgccgt ccaggtcgcc gcgacgcatc
     1261 ttcgagacgc cgaggctgtg cagcgcccat ccggcaccga agcggtcgtc gagtcggcgg
     1321 taggcctcga ggctctcctc ggcggcccgc tcggcgcggt ccaggtcacc gagacggtag
     1381 tagaggttgc tcagacccca cgtgaccttc gccagtccgg cctggtcgcc gagctcgccg
     1441 aagagacgga ggcttccctc gagcaggtcc cgcgcgcgat cgagatcctc ttgcccaccc
     1501 gtgacgggaa cgtggtacgg gaacgacagg ttgtacagcg cctccgcctc gccgcgcttg
     1561 tccccgagct cccggtacag cgtcagcatc tccccgtagt gcaacgcgca cgcggcccag
     1621 tcacccatcc agtaggcgac gccacccgcg ccctcgagcg cctcggccag cgcccgcggc
     1681 tgctccctcg cctcggggcg cgccaccagc tcctccaggc gcgttcgtgc ctcccgcagg
     1741 tgccctcgga actgccagaa ccgccacagg gccgccgaga gccgaaggcg aaactcgatg
     1801 tcggaggccc ggtccaggac tgcgcggatg ttgtcgtgct cccgctcgag cgcgtcgagc
     1861 cagtcggcct gccgcgggcc tagcagctcg ggccgggcgc gctccgcgag gccgaggaag
     1921 aactcctggt gccgccgcgc gatatcggct tgctcccgac cttcggccag ccgctcgccg
     1981 gcgtagtcgc ggatcgtgaa cagcatgaag aacctcggct cgccgctcgg cacctcggtc
     2041 tgacgcagga ggctcttgtt gacgagcgat tcgatgccgt cgaacgtgtc gagcccgagc
     2101 tccgcgtcgg ggttgcacac ctcctcggcg gcttcgagcg tgaagccacc gcggaagatc
     2161 gcgagccgcg agaagaaccg ccgctccggc tcgtcgagta agtcgtagct ccacgcgatc
     2221 gcgtcccgga gggtctgctg acgggccggc cggtcgcgcg cgccacccac gaggagcgaa
     2281 agccgctctt cgagccgcga caggatcgcc tgcggggaga ggagcttgat ccgcgccgcg
     2341 gcgagctcga tcgcgagcgg gagcccgtcg aggcgggatg tgatctcggc gacggccggc
     2401 gcgttctcgt tcgtgatcga gaagcctgtc ttcaccgccg tcgctcggtc gatgaacagc
     2461 cgaacggcct cgtactgcga cagcgcgtcc agcggcggaa ggcggcgcac atccggaagc
     2521 gcgagcggcg gcacctggta ttcgcgctcg ccatggagcc cgagcgcctc tcggctcgtc
     2581 acgaggaacg ttgcgccgga tgtaaccgcg agcagctggc cgatgtcgga cgccgcctcc
     2641 atgacctgtt cgaagttgtc gaggacgacc agcatgcgct tgtcacgcag gttctccgcg
     2701 acggcgtcga ccggagggcg gctcggatcc tcgcgcaatc cgagagcctg ggcgatggtc
     2761 ggcaccacga gcgcaggatc acggagcggt gacagcgcga cgaaggccgc gccgtcgtcg
     2821 aagtcgtcta cggtctccct cgcgacctcg atgctcagcc tcgtcttgcc ggtcccgcct
     2881 gggcccgtga gggtcaggag ccgcgtcttc gagaggatcg agatgacgtc gtccagctcg
     2941 cggcgccggc ccacgaacgt cgtcagctgc tccgggaggt tcgcgacgcg ccgctccagc
     3001 gtccgcagcg gcgggaactc cgcggggagc ccctcgacgt cgacctggaa tagatgctcg
     3061 ggctcgggaa ggtccttcag ccggtgctcg ccgagatcgc ggatcgtcgc gccctccgga
     3121 agcgtctgcc ggacgagcgc gcgcgtcggc tccgagacca cgatctggcc gccgtgcccc
     3181 gcggacatga tcctcgcggc gcggtggacg tcgatcccga cgtagttgtc gcctcccagg
     3241 agtccctcgc cggtgtggag gccgatgcgg gcccggaccg gtcctgcgtc gccccagtca
     3301 tgcaccgcga gcgcgcgctg cgccgcgacg gcggcggcaa cggcgtcggg tgcgctccgg
     3361 aacgcgacga agaacgagtc cccttccgtc gcgatctcca gcccgccgtg ccgttcgaac
     3421 gcgtctcgca tcagccgctg atgggtctcg agcagcggcg gatagtcctc gccgagggca
     3481 ttcagcagct tggtgctgcc ctcgatgtcc gagaacagga acgtgacggt cccggtcggc
     3541 agggtgtcgg tcggcacgcg gctcctccga ccggattatc ggcggtcgga gtg
//
LOCUS       DASZOB010000062         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_142420, whole genome shotgun sequence.
ACCESSION   DASZOB010000062 DASZOB010000000
VERSION     DASZOB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142420"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2729)
                     /locus_tag="VG709_01020"
     CDS             complement(<1..>2729)
                     /locus_tag="VG709_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWC31691.1"
                     /translation="PNPSGKWVAYATNVWESLALPSRHPGDNCNALPEDRQHPSCREG
                     DRDPDNDGPSGYTGPNGTSTAHGECPPQAGFIAGECFNNQLEWLDYYEDSMEEMLTDL
                     GVVVHRYPFHSPGRSDMGTERGGYLDGAGGQAYNISATIPGADHPEQTVLVSGHFDFT
                     DSGPAAAWDSAEGHTEVMRMAYIMADYYRKTGTRPSATLKFIPWDSEESGTFGSLDYV
                     QNNIPPGEEDKVRGYFNVDPCAGAYPAYRRGNPALGRVPEVMQLADPDAFATQPEVKA
                     RIEAFNERAETVVDEVFNYLDDTLTVAPGVEEPIFVSDEEAEEDGPGGLESQRDEIVT
                     AVGGLLIFSSDYANFSDVGIPIFNLFPDYFGPHADGTPGDNEGVAVLHTPRDNLRTIN
                     AMTNADQTGMTASEGWAKGMEMCSQIEAWYMLQPEMAGTQAADTDVVAYFEALPNEAL
                     VNQNVTFDARGTYQYANAATRSRVPQGELTYRWTFGDGTTANGRVVEHAFAEVGRYQA
                     QLRVTGRGGSTDTMTIPVEVVPSDFGAPALNPIPNDEAKDGSFNLTWGFESSRDGFRR
                     FSVQESRDYGTLFSDDAEGDIAETWNVELVSAEGDEAHRERTEPWQPSDSGTPKFRGN
                     QAHSGQRSYWTGVSPPNFSPSPLRVNSLMTLKEPVSLPESGDPMLDFWSLYQNEGDEQ
                     GRIEVAVDDGDPSTEPVWETVKVVQPPPTFLGDPHEPKICQPSEPETFLEDFVHETAN
                     LKRYLGKDVLIRFNYALGPEDRLSSQPCGWYMDDIRVASGTFEQIGTTEAEEFAVQGR
                     RNGTYAYRVLGVYNDGVRTAPSNVEVARVTEADVGPEKCPGHRNERGNHVVGTPRADT
                     LRGTAGRDVICGLGGGDTIRGKRGRDLLIGGRGPDSMYGKRGNDELLGNRGND"
BASE COUNT          349 a          932 c          953 g          495 t
ORIGIN      
        1 gtcgtttcct cgattaccga gcagctcgtc gttgccgcgc ttcccgtaca tgctgtcggg
       61 gccccggccg ccgatcagga ggtcgcgccc ccgcttcccg cggatcgtgt cgccgccgcc
      121 gagcccacag atgacgtcgc ggccggcggt tccacggagc gtgtcggcgc gaggggtccc
      181 gacgacgtgg ttcccgcgct cgttccggtg gccggggcac ttctcgggcc cgacgtcggc
      241 ctcggtgacg cgcgccacct cgacgttgct cggcgccgtg cgaacgccgt cgttgtagac
      301 gccgagaacg cggtacgcgt acgtcccgtt gcgtcgcccc tgcaccgcga actcctcggc
      361 ctcggtcgtg ccgatctgct cgaaggtccc ggaggcgacg cggatgtcgt ccatgtacca
      421 accgcacggc tgcgagctca gccggtcttc cgggccgagg gcgtagttga accggatcag
      481 cacgtccttc ccgaggtacc gcttgaggtt cgcggtctca tggacgaagt cctcgaggaa
      541 cgtctccggc tccgacggct ggcagatctt cggctcgtgc ggatcgccca ggaacgtggg
      601 cgggggctgg accaccttga cggtctccca cacgggctcg gtcgacggat cgccgtcgtc
      661 gacggcgacc tcgatgcggc cctgctcgtc gccctcgttc tggtacagcg accagaagtc
      721 gagcatcgga tcgccgctct cggggagaga gacgggttcc ttgagcgtca tcaacgagtt
      781 cactcggagc ggcgacgggc tgaagttcgg cggcgacacg ccggtccagt agctgcgctg
      841 tccggagtgc gcctggttgc cgcggaactt cggggtcccg ctgtcgctcg gctgccacgg
      901 ctccgtgcgc tctcggtgcg cctcgtcgcc ttcggcgctg acgagctcga cgttccaggt
      961 ttccgcgatg tcgccctccg cgtcgtcgct gaagagcgtt ccgtagtcgc ggctctcctg
     1021 gaccgagaac cgccggaagc cgtcgcgcga gctctcgaag ccccatgtga ggttgaagct
     1081 gccgtccttc gcctcgtcgt tcgggatcgg gttgagcgcc ggggccccga agtcggacgg
     1141 caccacttcg accgggatcg tcatggtgtc ggtgctgccg ccccggccgg tcaccctcag
     1201 ctgcgcctga tagcggccga cctcggcgaa cgcgtgctcg acgacgcggc cgttggccgt
     1261 cgtcccgtcg ccgaacgtcc agcggtacgt gagctctccc tgcgggacgc gcgagcgcgt
     1321 cgccgcgttg gcgtactggt acgtgccgcg cgcgtcgaag gtcacgttct ggttgacgag
     1381 cgcctcgttc gggagcgcct cgaagtacgc gacgacgtcg gtgtcggccg cctgcgtccc
     1441 ggccatctcc ggctggagca tgtaccaggc ctcgatctgc gagcacatct ccatgccctt
     1501 ggcccacccc tcggacgccg tcatgccggt ctggtcggcg ttcgtcatcg cgttgatcgt
     1561 ccggaggttg tcgcgcgggg tgtggagcac ggcgacgcct tcgttgtcgc cgggcgtgcc
     1621 gtcggcgtgc ggtccgaagt agtccgggaa caggttgaag atcgggatgc cgacgtcgga
     1681 gaagttcgcg tagtcgctcg agaagatcag caggccgccg acggccgtca cgatctcgtc
     1741 tcgctgcgac tccaggccgc cgggtccgtc ttcctcggcc tcctcgtcgg acacgaagat
     1801 cggttcctcg acgcccggag cgaccgtgag cgtgtcgtcg aggtagttga agacctcgtc
     1861 gacgacggtc tcggcgcgct cgttgaacgc ctcgatacgg gccttgacct ccggctgcgt
     1921 cgcgaacgcg tccgggtcgg cgagctgcat gacctcggga acccttccga gcgccgggtt
     1981 cccgcgccga tacgccgggt acgcgccggc gcacgggtcg acgttgaagt agccgcggac
     2041 cttgtcctcc tcaccgggcg ggatgttgtt ctgcacgtag tcgagcgatc cgaacgtgcc
     2101 cgactcctcg gagtcccacg ggatgaactt cagggtcgcg gacggccggg tcccggtctt
     2161 ccggtagtag tcggccatga tgtaggccat ccgcatgacc tcggtgtgac cctccgccga
     2221 gtcccacgcg gcggccgggc cggagtcggt gaagtcgaag tggccgctga cgagcacggt
     2281 ctgctcgggg tggtcggcgc ccgggatcgt cgcggagatg ttgtaggcct gcccgccggc
     2341 gccgtcgagg tacccgccgc gctcggtccc catgtcgctc cggcccggcg agtggaacgg
     2401 gtatcggtgg accacgacgc cgaggtcggt gagcatctcc tccatcgagt cctcgtagta
     2461 gtcgagccac tcgagctggt tgttgaagca ctcgccggcg atgaacccgg cctgcggcgg
     2521 acactcaccg tgcgcggtcg acgtcccgtt cggcccggtg tacccggacg ggccgtcgtt
     2581 gtctgggtcc ctgtcgccct cacgacagct cgggtgctgc cgatcctcgg gaagggcgtt
     2641 gcagttgtcc ccggggtggc gggaggggag cgccagcgac tcccagacgt tcgtggcgta
     2701 cgcgacccac ttaccagatg ggttcgggt
//
LOCUS       DASZOB010000063         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_92078, whole genome shotgun sequence.
ACCESSION   DASZOB010000063 DASZOB010000000
VERSION     DASZOB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92078"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1629)
                     /gene="metG"
                     /locus_tag="VG709_01025"
     CDS             complement(91..1629)
                     /gene="metG"
                     /locus_tag="VG709_01025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWC31692.1"
                     /translation="MKDTFYVTTPIYYPNDVPHIGHAYTTVAADFVARFHRLLGERVF
                     FLTGTDEHGKKVPQAAREQGLDPQTFVDRMEPRWRKVWELLDISNDDYIRTTEPRHEA
                     AVTKLLTGVRENGRDDIYLGRYEGLYCVSCEAYYTEDELVDGMCPIHGRPVEVLSEEN
                     YFFRLSAYQDRLLEHYERHPEAIEPEARRNEVLSVIKGGLRDFSISRTSFAWGVPIPW
                     DGDHVTYVWFDALTNYITAAGYADDGDRFSRIWPADVHFIGKDIVRFHAIYWPAMLMA
                     AGVEPPRQVWAHGFLLVGGEKMSKTKLTGIHPSEIVEHFGSDAYRYHFLREVQFGQDG
                     SFSWEAMVARYNADLANGLGNLASRTLAMLTSYFDGVVPESPPGTALESRLAETAEEA
                     SRRCEERVLDMDLAGGLASVWEFVGEVNRYLVEVAPWKLAKDEGSREELARCLYASAE
                     SLRIIAVLVSPVMPGAAARLWDQLGIPEPLATKRLPEAVRWGGLAAGTVVRRGDALFP
                     RLER"
     gene            complement(1655..2491)
                     /gene="rsmI"
                     /locus_tag="VG709_01030"
     CDS             complement(1655..2491)
                     /gene="rsmI"
                     /locus_tag="VG709_01030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWC31693.1"
                     /translation="MEGTGTLWLVATPIGNLADISQRARDVLASVDVIACEDTRRTRG
                     LLSHLGIAAPKLVALFDANERRRIPEILRRLTAGVDVALVTDAGTPAISDPGYRLVAA
                     CAEEGIDVDVVPGPSAAVAALVLSGLPTDRFAFEGFLPSRAGQRRARLAAIADEERTL
                     VLYEAPHRVLALLRDAHDALGDRRAAVVRELTKVHQEVLRATLSELAGRLADREIRGE
                     FVVVIAGAVAERASADPAEIAARVERLTAEGMSRRDAAARVARETGASRREAYEASLR
                     RR"
BASE COUNT          394 a          973 c          912 g          389 t
ORIGIN      
        1 catccgcgac gagcgacgcc gcgtcgccct ccatgaggaa gaggtggcag tgcgtgtcca
       61 cgagcgccgt ccgcgtcctt tccaccgagc tcatcgttcg agacgcggga acagcgcgtc
      121 gccccgtcga acgaccgtgc cggccgcgag cccgccccat cgcaccgcct cgggcagtcg
      181 cttcgtcgcg agcggctcgg ggatcccgag ctggtcccag agccgagccg cggcacccgg
      241 catcaccggt gagacgagga ccgcgatgat ccggagcgac tccgcgctgg cgtagaggca
      301 ccgcgcgagc tcttcccgcg agccttcgtc cttcgcgagc ttccacggcg caacctcgac
      361 gaggtaccgg ttgacctcgc cgacgaactc ccagaccgac gcgagccctc cggcgaggtc
      421 catgtccagc acgcgctcct cgcagcggcg cgacgcctcc tcggccgtct ccgcgagccg
      481 cgactcgagg gccgtccccg gcggggactc gggcacgacc ccgtcgaagt agctcgtgag
      541 catcgcgagg gtccgcgacg cgaggttccc gaggccgttc gcgaggtcgg cgttgtagcg
      601 ggcgaccatc gcctcccacg agaagctccc gtcctggccg aactgcacct cgcgcaggaa
      661 gtggtagcgg tacgcatccg atccgaagtg ctcgacgatc tcgctggggt ggatccccgt
      721 cagcttcgtc ttgctcatct tctcgccgcc gacgagaagg aacccgtgag cccacacctg
      781 ccgcggcggc tccacgcccg ccgccatgag catcgccggc cagtagatcg cgtggaaccg
      841 cacgatgtcc ttgccgatga agtggacgtc ggcgggccag atgcgcgaga aacggtcgcc
      901 gtcgtcggcg tacccggccg cggttatgta gttcgtgagc gcgtcgaacc acacgtacgt
      961 cacgtgatcc ccgtcccacg ggatcggcac gccccacgcg aacgacgtcc gcgagatcga
     1021 gaagtcgcgg agcccgccct tgatcaccga caggacctcg ttgcggcgcg cctcgggctc
     1081 tatcgcctcg gggtgccgct catagtgctc gagcagccgg tcctggtacg cggaaagtcg
     1141 gaagaagtag ttctcttccg agaggacctc gacgggcctg ccgtggatcg ggcacatccc
     1201 gtccaccagc tcgtcctccg tgtagtacgc ctcgcagctc acgcagtaga ggccctcgta
     1261 ccggccgagg tagatgtcgt cgcgcccgtt ctcgcgcacg cccgttagga gcttcgtgac
     1321 tgcggcctcg tgcctcggct ccgtcgtgcg gatgtagtcg tcgttggaga tgtcgaggag
     1381 ctcccacacc ttgcgccagc gcggctccat ccgatcgacg aatgtctgcg ggtcgagccc
     1441 ctgctcgcgc gcggcctgcg gcaccttctt cccgtgctcg tcggtgcccg tgaggaagaa
     1501 cacccgctcg cccaggaggc ggtggaagcg cgcgacgaaa tcggcggcga ccgtcgtgta
     1561 cgcgtggccg atgtgcggga cgtcgttcgg atagtagatc ggcgtcgtga cgtagaacgt
     1621 gtccttcatc gaaggagcga ttctcgcact tccgtcagcg gcgcctcagc gacgcctcgt
     1681 acgcctcgcg gcgcgacgcg ccggtctcgc gggcgacgcg tgcggcggcg tctcgccgcg
     1741 acatcccctc agccgtgaga cgctccacgc gcgccgcgat ctccgcgggg tcggccgacg
     1801 cccgctccgc cacggccccg gcgatgacca cgacgaactc gccgcggatc tctcggtcgg
     1861 cgagccgtcc cgcgagctct gacagcgtgg cgcggaggac ctcctggtgc accttcgtca
     1921 gctcgcggac cacagcggcc cggcggtccc ccagcgcgtc gtgcgcgtcg cgcagcagcg
     1981 cgagcacgcg gtgcggcgcc tcgtacagga ccagcgttcg ctcctcgtcc gcgatcgccg
     2041 cgaggcgcgc gcgccgctgg ccggctcgcg acggcaggaa cccctcgaac gcgaagcggt
     2101 cggtcggcag gccagacaac acgagcgccg cgacggccgc cgacggcccc ggaaccacgt
     2161 cgacgtcgat gccctcctcg gcgcacgcgg caacgagacg gtagccggga tcggatatgg
     2221 ccggcgttcc cgcgtccgtg acgagcgcga cgtcgacccc ggcggtgagc cgccgcagga
     2281 tctcggggat gcgccggcgc tcgttcgcgt cgaacagcgc gacgagcttg ggggcggcga
     2341 tgcccaggtg cgacaggagc ccgcgcgtcc ggcgcgtgtc ctcgcacgcg atcacgtcga
     2401 cactcgcgag gacgtctcgc gcacgctgcg atatgtcggc gaggttgccg atcggcgtag
     2461 cgacgagcca gagcgtgccc gtaccttcca tcgacgtgac gatacccgcg ccgagctacg
     2521 ccggcggagg catgaacgcg tcgaacgctc cgcgaagcgc cgcggcgagg aaggtgtcgt
     2581 gcggcggtga cgcccccgcg atgatgcgcc ggagcgcatc gagcgccgcc cgtgaggtcg
     2641 tgatcccggt gtgggtcgcg tccaccac
//
LOCUS       DASZOB010000064         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_21427, whole genome shotgun sequence.
ACCESSION   DASZOB010000064 DASZOB010000000
VERSION     DASZOB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21427"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VG709_01035"
     CDS             <1..1423
                     /locus_tag="VG709_01035"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWC31694.1"
                     /translation="LEKYLEEGELSEQELTSGVKAGLAEAKFAPVVCGSAAKAIGIDR
                     LADLFVDTFPSPSDRPPVKVMTKEGEERERPVDPDAPLAAFVFKTISDPYVGRINVFR
                     VYSGRVRPDVTVHNATQGDDERVHQLFTLRGKEHESVSEVPAGDIGAIAKLQNTHTGD
                     TLSTKDEPVTLPAIAWPEPLYALALEPKTQGDEEKLSTALARLREEDPTFRVERSSET
                     HETVAYGMGEAHVDVALERMKRKSGVDVATHPAKLPYKETIRGTAKAIGRHVKQSGGH
                     GQYGICHIEISPLERGRGFEFVNKIVGGAIPSQFIPSVEKGIQKAMTEGVLTGYPMVD
                     VQVVLYDGKFHTVDSSDMAFQIAGSLALKEGAQQAGVVLLEPIVDVEVVVPESYTGDI
                     IGDLNGKRGRVLGMDPTGTGKTRIHAQAPQAEMTRYAVDLRSITGGRGAFTMRFSHYE
                     EVPSHLADRVVAEMQREKQEAHR"
     gene            1495..>1776
                     /locus_tag="VG709_01040"
     CDS             1495..>1776
                     /locus_tag="VG709_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC31695.1"
                     /translation="MAVRVLIVDDQEPFRLAARAVVEMTDGFEVVGEAETGEDSIDQA
                     RVLDPDLVLMDVNLPGIDGLEATRRILAETKRTVVLLVSTYEADEYAPRA"
BASE COUNT          305 a          600 c          622 g          249 t
ORIGIN      
        1 gctcgagaag tacctcgaag agggcgagct ctccgaacag gagctcacga gcggcgtgaa
       61 ggcggggctc gccgaggcga agttcgcgcc ggtcgtctgc ggctctgcgg cgaaggcgat
      121 cgggatcgac cgcctcgccg acctcttcgt cgataccttc ccgtcgccgt ccgaccgtcc
      181 gccggtcaag gtcatgacga aggaaggcga ggagcgcgag cgcccggtgg atcccgacgc
      241 gccgctcgcg gcgttcgtct tcaagacgat ttcggatccc tacgtcggcc ggatcaacgt
      301 gttccgcgtc tactccgggc gcgtccgtcc cgacgtgacg gtccacaacg ccacgcaggg
      361 cgacgacgag cgagtccacc agctgttcac gttgcgcgga aaggagcacg agtcggtgtc
      421 ggaggttccg gcgggcgaca tcggcgcgat cgcgaagctc cagaacacgc acacgggcga
      481 cacgctctcg acgaaggacg agcccgtgac gcttccggcg atcgcgtggc ccgagcccct
      541 ctacgccctc gcgctcgagc cgaagacgca gggcgacgag gagaagctct cgacggcgct
      601 cgcgcgactg cgcgaggagg atccgacgtt ccgggtggag cgctcgagcg aaacccacga
      661 gacggtcgcg tacggcatgg gcgaggccca cgtcgacgtg gcgctggagc ggatgaagcg
      721 gaaatccggc gtcgacgtcg ccacgcaccc ggcgaagctc ccgtacaagg agacgatccg
      781 cggcaccgcg aaggcgatcg gccgccacgt gaagcagagc ggtggccacg gccagtacgg
      841 catctgccac atcgagatct caccgctcga gcgcggccgg gggttcgagt tcgtcaacaa
      901 gatcgtcggc ggcgcgatcc cgtcgcagtt catcccatcg gtcgagaagg ggatccagaa
      961 ggcgatgacc gagggcgtcc tcaccggata cccgatggtc gacgtccagg tcgtcctcta
     1021 cgacggcaag ttccacaccg tcgactcctc ggacatggcg ttccagatcg ccgggtcgct
     1081 cgcgctcaag gagggggcgc agcaggccgg cgtcgtgctg ctcgagccta tcgtcgacgt
     1141 cgaggtggtc gtgcccgaga gctacaccgg cgacatcatc ggcgacctca acggcaagcg
     1201 cggtcgcgtc ctggggatgg acccgaccgg cacagggaag acgcggatcc acgcgcaggc
     1261 tccgcaggcc gagatgacgc gctatgcggt cgatctccgc tcgatcaccg gaggccgcgg
     1321 cgcgttcacg atgcggttct cgcactacga ggaggtgcct tcgcacctcg ccgaccgcgt
     1381 cgtcgccgag atgcagcggg agaagcagga ggcgcaccgg tagcctccgc ccgccggggc
     1441 ggtggtgctg gcacccttcc gagcagtcgg cgcgggtagc atcctggtct cgagatggcc
     1501 gtgcgcgtcc tcatcgtcga cgatcaggag ccgttccggc tcgccgcccg tgccgtggtc
     1561 gagatgaccg acggcttcga ggtggtgggc gaggccgaga ccggcgagga ctccatcgac
     1621 caggcgcgcg tcctcgaccc cgacctcgtc ttgatggacg tcaaccttcc cgggatcgac
     1681 gggctcgagg cgacgcggcg gatcctcgca gagacgaagc ggaccgtcgt gctcctcgtc
     1741 tcgacgtacg aagccgacga gtacgcgccc cgcgcc
//
LOCUS       DASZOB010000065         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_72014, whole genome shotgun sequence.
ACCESSION   DASZOB010000065 DASZOB010000000
VERSION     DASZOB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72014"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..855
                     /locus_tag="VG709_01045"
     CDS             118..855
                     /locus_tag="VG709_01045"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWC31696.1"
                     /translation="MTLPLPPDDIDRANERFERSAPEAVLRWVFDGSGASQIAIASAF
                     QAEGVCVIHMATRIRPDVPILFLDTGFHFEETLAFKRKLTERLGLNVVELTGRYTPDM
                     QAREFGPRLYERDPELCCRLNKVEPFTEALHGYDAWITALRRDSAPTRAGTPIVEQYE
                     LEPGRWMLKANPIATWNRRDAWSYLREHDLPNNPLYDLGYAQVGCAPCTRLVVAGEDE
                     RAGRWDGAAKVECGIHEHARAGRSARA"
     gene            complement(859..1587)
                     /locus_tag="VG709_01050"
     CDS             complement(859..1587)
                     /locus_tag="VG709_01050"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWC31697.1"
                     /translation="MSVRRQPYNAPSALRRVVFAVVVIAVLLLALDFGARFWAQSWFA
                     SRVQDTLDSDASTDIEFHGFPFLTSFAAGHFDRVEVSAGAVDARGLMLRSVHLDLDDV
                     TFPRGSMLSRGSGRIDVGSGRGEVQVPEHALSAYVQARGIPINVELLDGRVRVSTEVQ
                     VGPASASASATGPLRLSGRRLVFEPSEVDVDGGVAVPPAAVAFEVPVPRLAPGVEYRS
                     VVVEDRVARLLIEIEDAVLRVREG"
     gene            complement(1612..>2871)
                     /locus_tag="VG709_01055"
     CDS             complement(1612..>2871)
                     /locus_tag="VG709_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC31698.1"
                     /translation="AWEDLRDATLLMKLDPQYDDLARHVGRALAILSGRCPDATGIGP
                     QPESCPAGEPVERGYDGLAPSLRSVAEGLSRAEPGIARILDGLDRIDAGLARLESGLT
                     SGRPNLERLETGVGRMIAGLDRAVPGLTRLRRGLETGVSLVEEIGSDLIPEPGDEVAL
                     TASIVEAFPKLRRQLSLFVGEQGRATRMFVALSRPPYDARAPQTSRRIEEIAPLSLRE
                     TALEDADVFVAGTPTFFSDVADSSESSFNTIVWVVVLGIFLILVLLLRSVVSPLYLVA
                     TVVLSFASTLGLTVIVFQGLLGDAGVTWWLPAFLFVILVALGADYNIFLMGRIREEAA
                     SQPTRPAVADGLAATGRVITSAGMILAGTFAALLVSPLEGLVQFGFATTVGLLIDTFI
                     VRSLLVPSIAVLMGSASWWPSRRAARA"
BASE COUNT          474 a         1020 c         1031 g          346 t
ORIGIN      
        1 gtgtggagtg ctcgtctcag acatgctgcc tcgctcctgg agtcgtcggg ttcgaggagg
       61 atagcaatgc gtcggcggcc gacgcggcgt cgatatactc ggcgcggagg cccgactttg
      121 acccttcccc ttccacccga cgacatcgat cgcgcgaacg agcgcttcga gcgttccgcg
      181 cccgaggcgg tgctgcggtg ggtcttcgac ggatccggcg cctcgcaaat cgcgatcgcg
      241 tcggcgttcc aggcggaagg cgtctgcgtc atccacatgg cgacgcggat tcggccggac
      301 gtaccgatcc tgttcctcga cacggggttc cacttcgagg agacgctcgc gttcaagcgc
      361 aagctcaccg agcggctcgg gctcaacgtc gtcgagctga cgggccggta cacgcccgat
      421 atgcaggccc gcgagttcgg cccaaggctc tacgagcgcg acccggagct ctgttgccgg
      481 ctcaacaagg tggagccgtt caccgaggcc ctccacgggt acgacgcgtg gatcacggcg
      541 cttcgccgcg actcggcgcc gacgcgcgca gggacgccga tcgtcgagca gtacgagctc
      601 gagcccggtc gatggatgct caaggcgaac ccgatcgcga cgtggaaccg ccgcgacgcc
      661 tggagctacc tgcgtgagca cgaccttccc aacaacccgc tgtacgacct cgggtacgcg
      721 caggtgggat gcgcgccgtg cacccggctc gtggtcgccg gagaggacga gcgcgcgggg
      781 cggtgggacg gggccgcgaa ggtcgagtgc ggcatccacg agcacgcgcg ggccggacgc
      841 agcgcgcgcg cctgagcgtc agccctcccg gacgcggagc accgcgtcct cgatctcgat
      901 caagagccgg gcgacgcggt cctcgactac gacgctgcgg tactcgacac ccggcgcaag
      961 ccgcgggacc ggcacctcga acgcgacggc cgccgggggg acggcgacgc ctccgtcgac
     1021 gtccacctcg ctcggctcga acacgagccg gcggcccgag agccggagcg gccccgtcgc
     1081 cgaggcggag gccgaggccg gcccgacctg aacctcggtc gagacgcgga cgcgcccgtc
     1141 gagcagctcg acgttgatcg ggatcccccg ggcctggacg tacgcggaca gcgcgtgctc
     1201 gggaacctgg acctcgccgc gtccggaccc gacgtcgatc cgcccgcttc cgcgggacag
     1261 catggacccc ctcgggaacg tcacgtcgtc gagatcgagg tgaaccgagc gcagcatcag
     1321 cccccgcgcg tcgaccgcgc cggcggagac ctcgacgcgg tcgaagtgcc cggccgcgaa
     1381 gctcgtcagg aacgggaagc cgtggaactc gatgtcggtc gacgcgtcgc tgtcgagcgt
     1441 gtcctgcacc cgggaggcga accacgactg cgcccagaac ctcgcgccga agtcgagcgc
     1501 gagcagcagc acggcgatca cgacgacggc gaagacgacg cgtcgcaggg cgctcggggc
     1561 gttgtacggc tgacggcgga ccgacatgcg cgtcagccta gcggcgcgcg gtcaggcgcg
     1621 cgccgcgcgg cgcgacggcc accagctcgc ggaccccatg agcaccgcga tcgacggcac
     1681 gaggaggctg cgcacgatga acgtgtcgat caggaggccg acggtcgtag cgaacccgaa
     1741 ctgcacgagc ccctcgagcg gcgagacgag gagcgccgcg aacgtgccgg ccaggatcat
     1801 cccggccgag gtgatgaccc gacctgtcgc ggccagcccg tccgcgaccg ccgggcgcgt
     1861 cggctgcgac gccgcctcct cgcggatccg ccccatcagg aagatgttgt agtcggcccc
     1921 gagcgcgacg aggatcacga agaggaacgc cggcagccac cacgtcaccc ccgcgtcgcc
     1981 gagcagaccc tggaagacga tgacggtgag gccgagcgtc gaggcgaacg acaggacgac
     2041 ggtcgcgacc aggtacagcg gcgacacgac gctgcgcagg agcagcacga ggatcaggaa
     2101 gatgccgagg acgacgaccc acacgatcgt gttgaacgag gactcggacg agtccgcgac
     2161 gtcggagaag aacgtcggcg tcccggcgac gaagacgtcg gcgtcctcga gagccgtctc
     2221 gcggagcgag agcggcgcga tctcctcgat ccgccgcgac gtctggggcg cccgagcgtc
     2281 gtagggcggc cgcgagagcg ccacgaacat ccgcgtcgcc ctcccctgct cgccgacgaa
     2341 cagcgagagc tgccgccgga gcttcgggaa cgcctcgacg atcgacgccg tcagcgcgac
     2401 ctcgtcgccg ggctcgggga tgaggtcgga gccgatctcc tccaccaggg agacgcccgt
     2461 ctccagccca cgccgcagcc tggtgagccc cgggaccgcg cgatcgagcc cggcgatcat
     2521 cctgccgacg cccgtctcga ggcgctccag gttgggacgc ccggacgtca gccccgactc
     2581 gagccgagcg aggccggcgt cgatgcggtc gagcccgtcg aggatccgcg cgatgcccgg
     2641 ttcggcgcgc gagagccctt cggccacgct gcggagactg ggggcgagcc cgtcgtaccc
     2701 ccgctcgacc ggttcaccgg cgggacagct ctcgggctgc ggcccgatgc ctgtggcgtc
     2761 ggggcaccgc ccggagagga tcgcgagcgc ccgtccgacg tgacgggcga gatcgtcgta
     2821 ctgcgggtcg agcttcatga ggagcgtcgc gtcacggagg tcttcccacg c
//
LOCUS       DASZOB010000066         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_131864, whole genome shotgun sequence.
ACCESSION   DASZOB010000066 DASZOB010000000
VERSION     DASZOB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131864"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VG709_01060"
     CDS             complement(<1..1668)
                     /locus_tag="VG709_01060"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWC31699.1"
                     /translation="MAPDGPATDAALADERDAVEHPRLRPEVTPTVITELSSRVLATK
                     EGEMFLYSDTEGNLDDRTELGLGLYFEDTRFLSHLRMRVSGRDPVLLSSSSERGYMSY
                     VDLTNPDLYDGEELAIPQQTLNIRRIRTINGRLFERIRVKNYNAFPVRLTIELTFAAD
                     FADLFGVRGIVRESRGHFRPPDASGRTITFSYVGLDDVPRETRIEFGSEPEEVGVRGD
                     VAWARFVLYLSAHQTKLVSMTATPVVESRSAPSTEFDVAVHQLRRSYEEWERRCTNIK
                     TDNELFDQLLTRGIRDLRALYTPVDGGSVLAAGIPWYVSPFGRDALITSHQLLMINPD
                     CARDALDVLARLQGTKVDPWRDEEPGKILHEVRRGELARSGEIPHTPYYGSVDSTPWF
                     LVVLAQYFRWTGDVDFVKTMMPAAEAALAWIDEYGDRDGDGFVEYESRSPRGLRNQGW
                     KDSWDSVVHADGRLAEPPIALAEVQAYVYLAKTRVADLYEALGDAERAAELRRQAIAL
                     RARFNEAFWIEEEQYFAEALDRDKRQVRSIVSNAGHGLYCDVVDPEKA"
     gene            complement(1714..>1909)
                     /locus_tag="VG709_01065"
     CDS             complement(1714..>1909)
                     /locus_tag="VG709_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWC31700.1"
                     /translation="LPLVPAARALFEELRDRGVPVCVAGAGPTLLAFEADGHSVPELG
                     PGWTALRTAVRADGATIEPA"
BASE COUNT          273 a          683 c          675 g          278 t
ORIGIN      
        1 ggccttctcc ggatcgacga cgtcgcagta cagcccgtgt ccggcgttcg agacgatcga
       61 gcgcacctgc cgcttgtcgc ggtcgagcgc ctcggcgaag tactgctcct cctcgatcca
      121 gaacgcctcg ttgaaccggg cccgcagcgc gatggcctgc cgccgcagct cggcggcgcg
      181 ttcggcgtcg cccagcgcct cgtagaggtc cgcgacgcgg gtcttcgcga gatacacgta
      241 ggcctggacc tccgccagcg cgatcggcgg ttcggcgagc cggccgtccg cgtggacgac
      301 ggagtcccac gagtccttcc acccctggtt ccgcaggccc cgcggggagc gcgactcgta
      361 ctccacgaac ccgtcgccgt cacggtcgcc gtactcgtcg atccacgcga gggccgcctc
      421 cgcggccggc atcatcgtct tgacgaagtc gacgtcgccc gtccagcgga agtactgcgc
      481 aaggacgacg aggaaccagg gcgtcgagtc caccgacccg tagtacgggg tgtggggaat
      541 ctcgcccgag cgcgccagct ccccgcgccg gacctcgtgc aggatcttcc ccggctcctc
      601 gtcgcgccac gggtcgacct tcgtcccctg cagccgcgcg aggacgtcga gcgcgtcccg
      661 cgcgcagtcg gggttgatca tcagcagctg gtgcgacgtg atgagcgcgt cccgcccgaa
      721 ggggctcacg taccagggga tgccggcggc gagcacgctc ccgccgtcga ccggcgtgta
      781 cagcgcccgc aggtcgcgga tcccgcgcgt cagcagctgg tcgaacagct cgttgtcggt
      841 cttgatgttg gtgcagcgcc gctcccactc ctcgtaggag cggcggagct ggtgcacggc
      901 gacgtcgaac tcggtcgagg gtgcagaccg gctctcgacg acgggcgtcg cggtcatcga
      961 cacgagcttc gtctggtgcg ccgagaggta cagcacgaac cgcgcccagg cgacgtcgcc
     1021 gcggacaccg acctcctccg gctcgctccc gaactcgatc cgcgtctcgc gcggcacgtc
     1081 gtcgaggccg acgtaggaga acgtgatcgt gcgcccggac gcgtccggcg gtcggaagtg
     1141 gcctcgcgac tcgcggacga tgccacggac cccgaacagg tccgcgaagt cggccgcgaa
     1201 cgtcagctcg atcgtcaggc gcacggggaa cgcgttgtag ttcttcacgc ggatccgttc
     1261 gaacaggcgg ccgttgatcg tccggatcct ccggatgttc agcgtctgct gcgggatcgc
     1321 gagctcctcg ccgtcgtaga ggtcggggtt cgtcaggtcg acgtaggaca tgtagccccg
     1381 ctcggacgac gaggagagca gcaccggatc gcgccccgag acgcgcatcc gcaggtgcga
     1441 cagaaagcgc gtgtcctcga agtacagccc gagccccagc tccgtccggt cgtcgaggtt
     1501 cccctcggtg tccgagtaca ggaacatctc gccctccttg gtcgcgagga cgcgcgagga
     1561 gagctcggtg atgacggtcg gcgtgacctc ggggcgcagg cgaggatgct cgacggcgtc
     1621 gcgctcgtcg gcgagcgcgg cgtcggtcgc cgggccgtca ggcgccacgc cgggtcaccc
     1681 gctcagtgta tggccgcggg cgaggcgcac cgctcaggcc ggctcgatcg tcgcgccgtc
     1741 ggctcgcacg gccgtccgga gcgcggtcca cccgggcccg agctcgggga cgctgtggcc
     1801 gtccgcctcg aacgcgagca gcgtgggtcc ggcgccggcg acgcagacgg gcactcctcg
     1861 gtcgcgcagc tcctcgaaga gcgcgcgcgc cgccggaacg agcggcagc
//
LOCUS       DASZOB010000067         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_62021, whole genome shotgun sequence.
ACCESSION   DASZOB010000067 DASZOB010000000
VERSION     DASZOB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62021"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VG709_01070"
     CDS             <1..218
                     /locus_tag="VG709_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWC31701.1"
                     /translation="VTPGPGLLAILAGLAVLATEFVWAERLYERAKSRGRAAYDLATS
                     SKIRIAFSVSLTLLGIAGAVWIWLAYR"
     gene            complement(226..1440)
                     /locus_tag="VG709_01075"
     CDS             complement(226..1440)
                     /locus_tag="VG709_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWC31702.1"
                     /translation="MTLGSMARGGWVSRITSTTAHVAAPTLAAILVVASTPATASHRA
                     SIRFGAPVVADDVQYTVSTAIAPVLGDDRRPDVLVTGLELGRGCYLLAYRQRRNGSLR
                     PPRHLPVRCSHGADVSTGDIDGDRDVDVAVTAWEGILLLKQRAGRLRDRVMVPIEVGD
                     EHAAPYIVEIADLDRDGRRDLVTSTTDGVFVLYNRRAAFDTVQIDPNYQFALVVHDVT
                     GDRRLDIIGCGDDIYTNCGRLNVFEQRADGTFAEEPYEGIPPQYRSFDAIAAGDVTGD
                     GRADLVVTLDHDRPLNIVPQLPSGDLGERIRLDIAVNAQSVAIRDMNLDGLKDIVLVH
                     SGNGSRSSARLGVVLQLPQGQGWSRERRFEASGWAYHLRSTTLAIGQLNRDRHPDAAF
                     ADGEFLAVFQQE"
     gene            1540..2289
                     /gene="recO"
                     /locus_tag="VG709_01080"
     CDS             1540..2289
                     /gene="recO"
                     /locus_tag="VG709_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWC31703.1"
                     /translation="MPGLYKEQGIVLGSVKLAEADKIVTILTQGSGKVRGVAKGIRRT
                     TSRFGARLEPFTHVNVMLYRGRNLDTVTQAEIIQPFTAIREDFALFSAGEAMLEATDR
                     VAEEHERNVRLFLLLLQGLRALETRPQDPVAVAEAFLLRLLNLSGFAPSLAACAVCGR
                     KPVERFSASQGGAVCDDCRERDARRATPDALAWLAELSRTDLETAGNRTPVPAVRVEA
                     RALLYGFAEYHLERRIKSLPLLAASSRRQQG"
     gene            2286..>2884
                     /gene="uppS"
                     /locus_tag="VG709_01085"
     CDS             2286..>2884
                     /gene="uppS"
                     /locus_tag="VG709_01085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWC31704.1"
                     /translation="MSSYLHDLDPSRIPRHVAIVMDGNGRWAAQRGLPRTNGHEAGER
                     ALYDVVEGALDVGLPYLSVYAFSTENWRRPPAEVRFLMNFNRRLIRARRDELDERGVR
                     IRFLGRRDRRLPRSVQREMDDAERMTRGNTKLTLVVALNYGGRAEIVDAVRSLLDDHD
                     AGRLRGTRITEASIARRLHLPDVPDPDLVIRTSGEQRIS"
BASE COUNT          448 a         1006 c          976 g          454 t
ORIGIN      
        1 tggtgacacc gggccccggg ctcctggcga tcctcgcggg gctcgcggtg ctcgcgactg
       61 agttcgtctg ggccgagcgc ctctacgagc gcgcgaagag ccgggggagg gccgcgtacg
      121 acctcgcgac ctcctcgaag atccggatcg ccttctcggt ctcgctcacg ctgctcggga
      181 tcgccggcgc ggtgtggatc tggctggcgt accgctagcc gttcctcact cctgttgaaa
      241 gaccgcgagg aactctccgt cggcgaacgc cgcgtcgggg tgacgatctc tgttcaactg
      301 cccgatggct agcgtggtcg atctcaggtg gtaggcccaa cccgaggcct cgaaccggcg
      361 ctcccgcgac cagccctgtc cttggggaag ctgaagcaca acgccgagtc gcgccgaaga
      421 ccggctcccg tttccgctat gcacgagaac gatgtccttg aggccgtcca ggttcatatc
      481 gcggatcgct acggactggg cattgaccgc gatatccaag cggatacgtt cgccgaggtc
      541 tcccgacggg agctggggca cgatgttcaa cggccggtca tggtcaagcg tcacgacgag
      601 atcggcgcgt ccgtcgcccg tcacgtcgcc ggcggcgatc gcgtcgaacg atcggtactg
      661 aggagggatc ccctcgtacg gctcctcggc gaacgtgccg tccgctcttt gctcgaagac
      721 attgagccgt ccacagttcg tgtagatgtc gtccccgcac ccgatgatgt cgagccgccg
      781 gtctcctgtc acgtcgtgca cgacgagtgc gaactgatag ttcgggtcga tctgcacggt
      841 gtcgaacgcg gcccgccggt tatacaggac gaagacacca tccgtcgttg acgtgacgag
      901 gtcgcgacga ccatcgcggt cgaggtcggc gatctcgacg atgtacgggg cggcgtgctc
      961 gtcgcccact tcgatgggga ccatgacccg atcacgaagg cgcccggccc gctgcttcag
     1021 caggaggatg ccctcccatg ccgtcaccgc gacgtcgacg tcacgatccc catcgatgtc
     1081 gccggtgctg acatcagcgc cgtggctgca ccgtaccggg aggtgtctcg gaggtcgaag
     1141 cgaaccgttg cggcgttggc ggtatgccag taagtagcat ccgcgaccga gctccaagcc
     1201 ggtgaccagc acgtccggcc ttctgtcgtc gccgaggacg ggcgcgatcg cggttgagac
     1261 ggtgtactgg acgtcgtcag cgacgacagg tgccccgaag cggatgcttg ctcgatggct
     1321 cgcagttgcc ggagtcgacg cgacgacgag tatcgcggcc agtgtcggcg cggctacatg
     1381 agccgtcgtc gacgtgatac gggaaaccca gccgccgcgc gccatcgacc ccagagtcaa
     1441 ccgtaaatcg gagcagcgga caacggtctc accgtcggtt cgggagcgcg tccggcgagg
     1501 tcggcgcttc cttctccagg agacggtacg atttcgaccg tgccgggcct ctacaaggag
     1561 caggggatcg tcctcggctc cgtgaagctc gctgaggccg acaagatcgt cacgatcctc
     1621 acccagggct ccgggaaggt ccgcggcgtc gcgaagggca tccgccggac gacgagccgc
     1681 ttcggcgcgc ggctcgagcc cttcacccac gtgaacgtga tgctctatcg aggtcgcaac
     1741 ctcgacaccg tcacccaggc cgagatcatc cagccgttca cggcgatccg cgaggacttc
     1801 gcgctgttct cggccggcga ggcgatgctc gaggccaccg accgcgttgc ggaggagcac
     1861 gagcgcaacg tgcggctctt cctgctcctc cttcaagggc tccgggcgct cgagacgagg
     1921 ccgcaggacc ccgtcgcggt cgccgaggcg ttcctgctgc ggctgctgaa cctctccggg
     1981 ttcgccccga gcctcgcggc gtgcgcggtg tgtggccgga agcccgtcga gcgcttctcg
     2041 gcgtcgcagg gcggcgccgt ctgcgacgac tgccgagagc gcgacgcacg acgcgcgacg
     2101 cccgacgcgc tcgcgtggct cgccgagctc tcgaggacgg acctcgagac cgcgggcaac
     2161 cggacgccgg tcccggccgt ccgcgtcgag gcccgcgcgc tgctctacgg cttcgcggag
     2221 taccacctcg agcggcgcat caagagcctg ccgctgctcg ccgcgtcgtc gcgccgccag
     2281 cagggatgag ctcgtacctc cacgacctcg acccgtcgcg catccctcgt cacgtcgcga
     2341 tcgtgatgga cggcaacggc cgctgggccg cgcagcgcgg cctgccgcgc acgaacggcc
     2401 acgaggccgg cgagcgcgcc ctctacgacg tcgtcgaggg ggcgctcgac gtcgggctgc
     2461 cgtacctcag cgtctacgcg ttctcgaccg agaactggcg ccggccaccg gcggaggtcc
     2521 gcttcctcat gaacttcaac cgccggctta tccgcgcgcg gcgcgacgag ctcgacgagc
     2581 gaggcgtgag gatccggttc ctcggccgcc gcgaccgccg gctgccgcgg tccgtgcagc
     2641 gggagatgga cgacgcggag cggatgacgc gtgggaacac gaagctcacc ctcgtcgtgg
     2701 cgctcaacta cggcgggcgt gcagagatcg tcgacgccgt ccggtctctc ctcgacgacc
     2761 acgacgccgg acggctgcgc ggcacgcgga tcacggaggc gagcatcgcg cgccgcctcc
     2821 acctccccga tgtcccggac cccgacctcg tgatccggac ctcaggggag cagcgcatct
     2881 cgaa
//
LOCUS       DASZOB010000068         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_193324, whole genome shotgun sequence.
ACCESSION   DASZOB010000068 DASZOB010000000
VERSION     DASZOB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193324"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VG709_01090"
     CDS             <1..193
                     /locus_tag="VG709_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HWC31705.1"
                     /translation="WEGGEVLRRVRETGSRARVVICTAEGLEETERRARDEGCVYLAK
                     PFTMEELDAAVGEAITALP"
     gene            complement(177..974)
                     /locus_tag="VG709_01095"
     CDS             complement(177..974)
                     /locus_tag="VG709_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWC31706.1"
                     /translation="MDDDLAFALELTDLADSITMARFGAPDLIVESKRDESPVTDVDR
                     EVERVLRGQIEAARPGDAVVGEELGARGEAASRWVIDPIDATKNYVRGIPVFATLIAL
                     QVDGVGKTAVISAPALGRRWWAARGAGAYVGRPTDTAGKRIRVSRVERLEDAQVCYSS
                     LRTWEDAGTGDRLLELQRRAWRTRGFGDFWMHCLVAEGAADVAVEAAVSLWDLAAIQV
                     LVEEAGGRFTDLGGRATPDGGSAVSSNALLHDEVLSIVSGPGATGGP"
     gene            1026..2096
                     /locus_tag="VG709_01100"
     CDS             1026..2096
                     /locus_tag="VG709_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HWC31707.1"
                     /translation="MRTGYADLPLHGGRAPAWLFGRMTALAREVSLAMVADEGVPGLL
                     RRLSDPVWFQAFGAVLGFDWHSSGVTTTVCGALKEGLRGVDAGVVVAGGKGRASRRTP
                     AEIAAACERWGGDASRLVRASRLSAKVDSSAVQDGFQVYHHTFLFTPDGAWAVVQQGM
                     NDASGMARRYHWFQPARFDADPHAAVAGSRADAVLNLVASEAEANRRTAAALGREEPH
                     AVTREIARMRTLEMPRRHAVLLSDLRPERLDRVLLRAYEAQPRTFTDLLAVPGIGAKG
                     LRALALVAELAYGEPASVRDPVSYAFAHGGKDRTPFPVDRETYDATIESLRRALSDAR
                     AGRGEKVEALRRLGRLEALAPR"
     gene            2093..>2358
                     /locus_tag="VG709_01105"
     CDS             2093..>2358
                     /locus_tag="VG709_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWC31708.1"
                     /translation="MRFLLARPDHLGDVLLTLPAASALKRAFPRSTVTYLVAPGSADA
                     PALSADVDDALTVEFPAPNDGRPPPVEVVDRAARRLRGRFDAAV"
BASE COUNT          287 a          871 c          877 g          323 t
ORIGIN      
        1 ctgggagggg ggggaggtgc tgaggcgggt ccgcgagacg ggatcgcgtg cccgcgtcgt
       61 gatctgcacg gccgagggcc tcgaggagac ggagcggcgt gcgcgcgacg aggggtgcgt
      121 gtacctcgcg aagccgttca cgatggagga gctcgacgcc gcggtcggcg aggccatcac
      181 ggccctcccg tagcccccgg tccggagacg atcgacagca cctcgtcgtg taagagggcg
      241 ttcgacgaca ccgcgctgcc gccgtcgggc gtcgcgcggc ctccgaggtc ggtgaaccgg
      301 ccgccggcct cctcgacgag gacctggatc gcggcgaggt cccacagcga cacggcggcc
      361 tcgaccgcga catcggccgc cccctcggcg acgaggcagt gcatccagaa gtcgccgaag
      421 ccgcgcgtgc gccacgctcg ccgctggagc tcgagcagcc ggtcaccggt cccggcgtcc
      481 tcccacgtgc gaaggctcga gtagcacacc tgggcgtcct cgaggcgctc gacccgcgag
      541 acgcggatac gcttgccggc cgtgtcggtc gggcgtccca cgtacgcgcc ggcgccgcgg
      601 gccgcccacc accggcgtcc gagcgcgggt gccgagatca cggcggtctt cccgacgccg
      661 tcgacctgga gcgcgatcag cgtcgcgaag accgggatgc cgcggacgta gttcttcgtc
      721 gcgtcgatcg ggtcgatcac ccatcgggat gccgcctcgc cgcgtgcgcc gagctcctcg
      781 ccgacgaccg cgtcgcccgg ccgggccgcc tcgatctggc cgcggagcac gcgctcgacc
      841 tcccggtcga cgtcggtcac cgggctctcg tcgcgtttcg attccacgat gaggtcgggc
      901 gccccgaagc gcgccatcgt gatcgagtcg gcgaggtcgg tcagctcgag cgcgaacgcg
      961 aggtcgtcgt ccaccggcgc gaggctacgt catcggcgtc gggggccgcc cgtacacttc
     1021 cgccgatgcg taccgggtac gccgaccttc cgctccacgg cggacgcgcc ccagcctggt
     1081 tgttcgggcg gatgacggcg ctcgcgcgcg aggtgtcgct cgcgatggtc gccgacgagg
     1141 gcgtccccgg cctcctccgc cggctctccg atcccgtgtg gttccaggcg ttcggtgcgg
     1201 tcctcgggtt cgactggcac tcgagcggcg tgacgacgac cgtgtgcggc gcgctgaagg
     1261 agggcctccg cggcgtcgat gccggggtcg tcgtggccgg agggaagggc cgcgcgtccc
     1321 gccggacgcc ggcggagatc gccgccgcgt gcgagcggtg gggcggcgac gcgtcgcggc
     1381 tcgttcgcgc gagccgcctc tccgcgaagg tggactcgtc ggccgtccag gacggcttcc
     1441 aggtctacca ccacacgttc ctgttcacgc cggacggcgc gtgggcggtc gtccagcagg
     1501 ggatgaacga cgcgagcggc atggcgaggc gataccactg gttccagccc gcacgcttcg
     1561 acgcggatcc gcacgccgcg gtcgcggggt cacgcgcgga cgcggtgctg aacctcgtcg
     1621 cgtccgaggc cgaggcgaac cggcgcaccg ctgccgcgct cgggcgggag gaaccgcacg
     1681 ccgtcacacg cgagatcgct cggatgcgaa cgctcgagat gccccgccgt catgcggtgc
     1741 tcctctcgga cctccgtccg gagcggctcg atcgcgtctt gctccgggcg tacgaggcgc
     1801 agccgcggac gttcacggac ctcctcgcgg tccccggcat cggcgcgaag ggtctccggg
     1861 cgctcgcgct cgtcgccgag ctcgcctacg gcgagccggc ctcggtgcgc gaccctgtgt
     1921 cgtacgcgtt cgcgcacggc ggcaaggacc gcacgccgtt ccccgtcgac cgcgagacgt
     1981 acgacgcgac gatcgagtcg ctgcgccggg cgctctctga cgcgcgcgcg gggcgcggcg
     2041 agaaggtgga ggccctccgc cgcctgggga ggctcgaggc gctcgcaccg cgatgaggtt
     2101 cctcctcgca cgaccggacc atctcggcga cgtgctcctc acgctgccgg cggcctcggc
     2161 tctcaagcgg gcgttcccgc ggtcgaccgt cacgtatctc gtggcgcccg gcagcgcgga
     2221 cgctccggcg ctctccgctg atgtcgacga cgcgctcacc gtggagttcc ccgcgccgaa
     2281 cgacggccgc cccccgccgg tcgaggtcgt cgaccgcgcg gcgcggcggc tgcgagggcg
     2341 cttcgacgcc gcggtgat
//
LOCUS       DASZOB010000069         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_92259, whole genome shotgun sequence.
ACCESSION   DASZOB010000069 DASZOB010000000
VERSION     DASZOB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92259"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..934)
                     /locus_tag="VG709_01110"
     CDS             complement(125..934)
                     /locus_tag="VG709_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HWC31709.1"
                     /translation="MAPEPRSTRRMGRISDEEGELSYATYLRIPELLDVQSPIAEPPA
                     HDELLFIVVHQAFELWFKEMLFELESARDAMFGGETFKPRHYLERVHAILRILIEQIR
                     VLETMSPQEFLEFRSNLSPASGFQSLQFREIEYVSGLRNPAYVNLAEEGPERERLRRR
                     LDEPTLWEGYRAVMEAEGLPMPADDEEGRRASLLKMMRDRDAFAELFALSEALIAYDE
                     LFSQWRFHHVLMVEREIGSKTGTGGSSGSAYLRTTLDKRFFPELWGIRSYL"
     gene            982..>2004
                     /locus_tag="VG709_01115"
     CDS             982..>2004
                     /locus_tag="VG709_01115"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWC31710.1"
                     /translation="MDERRGTTPERHDVLVVGAGPAGLAAGAMLSRRGAEVVLLDAAA
                     RVGDGWRRHYDRLRLHTVRWLSGLPGMPIPREYGKWVPRDRVADYLEAYARRFALQVR
                     TSAPVNRLDRDGGGWTAATPAGAIRADHVVVATGFNREPYLPGWPGRDGFRGELVHSS
                     AYRTPAPYRRRRVLVVGTGNSGAEIAVDLADAGAEVVLSVRTGPHIMRRDLFGFPSQT
                     LGVAMRRLPPRVVDRLQRGVQRVAIGDLERYGMPPPSRRGMYTRLLEESAVPILDVGL
                     VRLLKQRRVRVVAAVERLDGDDVVLADGSRVRVDAIVAATGFRRGLEPLVGHLGVLDD
                     RGRPLVH"
BASE COUNT          282 a          724 c          701 g          297 t
ORIGIN      
        1 tcgacgtacc gctcgagccg acgccgcgcg ccccgcgaga tcgggcccag ggaggcgctg
       61 atcaggtacg acttccgcgt tacgacgggg aactccccgc ggtagtcctc gagcggccgg
      121 acgctcacag gtagctcctg ataccccaga gctccgggaa gaacctcttg tcgagcgtcg
      181 tccggaggta cgcggacccg ctcgaccctc cggtcccggt cttgctcccg atctcgcgct
      241 cgaccatcag cacgtgatgg aaccgccact gcgagaacag ctcgtcgtac gcgatgaggg
      301 cctccgagag cgcgaagagc tcggcgaacg cgtcgcggtc gcgcatcatc ttgaggaggc
      361 tggctcggcg cccctcctcg tcgtccgcgg gcatcgggag gccttcggcc tccatgacgg
      421 cgcggtatcc ctcccacagc gtcggctcgt cgagtcgtcg acgcagccgc tcgcgctcgg
      481 ggccctcctc cgccaggttc acgtacgccg ggttccggag gccggagacg tactcgatct
      541 cgcggaactg cagcgactgg aagcccgaag ccggagagag gttcgaacgg aactcgagga
      601 actcctgcgg cgacatggtc tcgaggacgc ggatctgctc gatgaggatc cgcaggatcg
      661 catgcacgcg ctcgaggtag tgccgcggct tgaacgtctc gcccccgaac atcgcgtcgc
      721 gcgccgattc gagctcgaac agcatctcct tgaaccacag ctcgaacgcc tggtggacga
      781 cgatgaacag gagctcgtcg tgagcgggtg gctcggcgat cggcgactgg acgtccagaa
      841 gctcggggat gcgcaggtag gtcgcgtagg agagctcgcc ctcctcgtcg gagatgcgcc
      901 ccatcctccg cgtcgaccgc ggctcgggag ccatcgccgc gatattaccc ccgggccttc
      961 tcgtcggtag gctgacgtgc gatggacgag cgacgcggca cgacgccgga acgacacgac
     1021 gtgctggtgg taggcgccgg accggccggg ctcgccgccg gggcgatgct cagccgccgc
     1081 ggcgccgagg tcgtcctcct cgacgccgca gcgcgcgtcg gcgacggctg gcgccgccac
     1141 tacgaccgcc tgcgcctcca cacggtccgc tggttgtcag ggcttcccgg catgccgatc
     1201 ccacgtgagt acgggaagtg ggtcccccgc gaccgcgtcg ccgactacct cgaggcatac
     1261 gcacgtcggt tcgccctcca agtccggacg tcggcgcccg tcaaccgcct cgaccgtgac
     1321 ggaggtgggt ggaccgccgc gacgcccgcc ggtgcgatcc gagcggatca cgtcgtcgtc
     1381 gcgacggggt tcaaccgcga gccgtacctt cccggctggc cgggccgcga cgggttccgc
     1441 ggcgagctcg tccactcgag cgcctaccgg accccggcgc cgtaccgacg gaggcgggtc
     1501 ctcgtcgtgg ggaccggcaa ctcgggcgcg gagatcgccg tcgacctcgc cgacgccggc
     1561 gcggaggtcg tgctgtccgt ccgcaccggc ccgcacatca tgcgccgcga tctgttcggg
     1621 ttcccgtcgc aaacgctcgg cgtcgcgatg cgtcggctcc cgccgcgcgt cgtcgaccgg
     1681 ctccagcgtg gggtgcagcg cgtcgcgatc ggcgacctcg agcggtacgg catgccgccg
     1741 ccgtctcggc gagggatgta cacgcggctc ctcgaggaga gcgccgtgcc gatcctggac
     1801 gtcgggctcg ttcgactcct gaagcagcgc cgggtccggg tcgtggcggc cgtcgagcgg
     1861 ctcgacggcg acgacgtcgt gctagcggac ggctcacgcg tgcgcgtcga cgcgatcgtc
     1921 gcggcaacgg ggttccgtcg cggcctcgag ccgctggtcg gtcacctcgg cgtcctcgac
     1981 gaccgcggac gccccctcgt gcac
//
LOCUS       DASZOB010000070         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_183264, whole genome shotgun sequence.
ACCESSION   DASZOB010000070 DASZOB010000000
VERSION     DASZOB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183264"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VG709_01120"
     CDS             complement(<1..667)
                     /locus_tag="VG709_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC31711.1"
                     /translation="MGAVFGRDRELAHADTLLESAAGRSGSLLLEGEPGIGKTTVWRE
                     VVSRAEDRGFRVLAAQPAEAETKLALSAVADLLEGVPEPAFAALPDPQRRALEVALLR
                     VAPGAAAPEPRTLATGLRSLLAELTAEAPLLVAIDDVQWVDLSSARMLAFALRRLPDE
                     RVRWLFAQRLGVPTRLAVDELVPPESLERLTIGPLTLAALHHLLEERLHRPLSRSVLV
                     RVHG"
     gene            complement(722..1495)
                     /locus_tag="VG709_01125"
     CDS             complement(722..1495)
                     /locus_tag="VG709_01125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWC31712.1"
                     /translation="MSIRRVVLALAVSSLASCAAVEDAPVARSVPELARERSVVVERA
                     ATVAPRPLVETESDEMALTAAVDARRIGAFRRPHAPRPFEVFRNPNEYGYPVVFLVKR
                     TRGDWLEVYLPIRPNGATGWVRARHVTLSENPFRIEVDLSENRLLLRRAERVVMRERV
                     AVGTGGTPPPTGLFYTRMEHRTGDPGGVYGPFIQVLSAYSEVHFTFNGGNGEVGIHGT
                     NDPSLLGQDVSNGCIRMNNRAITRLTRLSPVGTPVRIRA"
     gene            complement(1492..>2344)
                     /locus_tag="VG709_01130"
     CDS             complement(1492..>2344)
                     /locus_tag="VG709_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31713.1"
                     /translation="VEAVRAEMSSSVAERLAEMEREISAAVTSAGDDQRDAAASVVRI
                     GEELRTEVERAVSSVRENVASLRSELERQAAALGEGMARVDRLGDAVETIGRRRAFRK
                     LVDAERDLHREQVALVEELTSAGETVTGAVRDIEARIADAAESVAAAAERNADEVGAI
                     REVRQVLESFPALLESRLTEAVDAVHADIVATGSRSEGSGDEIVSLREEIEALRSAVE
                     GSAKRAPATKLARDVKALQGRIDDLEASMRDTLAKEVAQRVRASIDRRVKSFLDEIRG
                     EVGKEGS"
BASE COUNT          322 a          872 c          842 g          308 t
ORIGIN      
        1 caccatggac gcgcacgagg acggagcgcg agagcggccg gtggagccgc tcctcgagca
       61 ggtgatggag ggccgccaac gtgagggggc cgatcgtgag gcgctccagc gactcgggcg
      121 ggaccagctc gtccacggcg agtcgagtgg ggacgccgag ccgctgagcg aacagccagc
      181 ggacccgctc gtccggcagg cgccggagcg cgaacgcgag cattctcgcc gaggagagat
      241 ccacccactg cacgtcgtcg atcgccacga gcagcggcgc ctccgccgtc agctccgcca
      301 ggagcgaccg gagaccggtg gcgagcgtgc gcggctccgg cgcggcggct cccggcgcta
      361 cgcggaggag cgcgacctcg agtgcccggc gctgtgggtc cggcaaggcg gcgaacgccg
      421 gctcgggcac tccctcgagc aggtcggcca cggcggacag cgcgagcttc gtctcggctt
      481 cggcgggttg ggcggcgagg acgcgaaagc cgcgatcctc cgcccgggac acgacctcgc
      541 gccagacggt cgtcttgccg atcccgggct cgccttcgag gagcagcgaa ccggatcgcc
      601 cagccgcaga ctcaaggagg gtgtctgcgt gggcgagttc gcgatcccgc ccgaaaactg
      661 cacccacgcc cggattgtcc gcccgccttg gcgcgaatgt aaagaactcc gacggccgcc
      721 gtcaggcccg gatccggacc ggcgtgccga ccggcgacag ccgcgtcagg cgggtgatcg
      781 cgcggttgtt catccggatg cagccgttgc tcacgtcttg cccgagcagc gacgggtcgt
      841 tcgtgccgtg gatcccgacc tcgccgttgc cgccgttgaa cgtgaagtgc acctccgagt
      901 acgcggagag cacctggatg aacggcccgt agacgcctcc ggggtcgccc gtgcgatgtt
      961 ccatccgcgt gtagaacagc ccggtcgggg gcggcgtccc gccggtgccg accgcgacgc
     1021 gctcgcgcat gacgacccgc tctgccctcc ggagcaagag ccggttctcg ctgaggtcca
     1081 cctcgatgcg gaacgggttc tccgagagcg tcacgtggcg agcgcggacc cagccggtcg
     1141 ctccgttcgg ccggatcggc aggtagacct cgagccagtc gccacgcgtg cgcttcacga
     1201 ggaagacgac cgggtagccg tactcgttgg ggttccggaa gacctcgaac ggccgcgggg
     1261 cgtgaggccg ccggaaggcg ccgatgcgcc gcgcatcgac ggcggccgtg agcgccatct
     1321 cgtcgctctc ggtctccacc aggggccgcg gggcgaccgt ggccgcgcgt tcgacgacca
     1381 cggaccgctc gcgggccagc tccggcaccg agcgcgcgac cggggcgtcc tcgacggcgg
     1441 cgcagctcgc gagcgacgag accgcgagcg ccagcaccac ccgccggatg ctcacgaccc
     1501 ctccttcccg acctcgccgc ggatctcgtc gaggaacgac ttcacgcgcc ggtcgatcga
     1561 cgcccggacc cgttgcgcga cctccttggc gagcgtgtcg cgcatcgagg cctcgaggtc
     1621 gtcgatccgg ccctggagcg ccttcacgtc gcgggcgagc ttcgtcgcgg gggcgcgctt
     1681 cgccgagccc tccaccgccg atcgcaacgc ctcgatctct tcgcggagcg agacgatctc
     1741 gtcgccggag ccctcggacc ggctgcccgt ggcgacgatg tcggcatgga ccgcgtcgac
     1801 ggcctcggtc aggcggctct ccaggagcgc ggggaacgac tcgagcacct gacgcacctc
     1861 gcggatggcg ccgacctcgt ccgcgttccg ctcggccgcc gcagcgacgc tttccgccgc
     1921 gtccgcgatg cgcgcctcga tgtcgcggac cgcgccggtg acggtctcgc cggcggacgt
     1981 gagctcctcc acgagcgcga cctgttcccg gtggaggtca cgctccgcgt cgacgagctt
     2041 ccggaaggcg cggcggcgcc cgatcgtctc gacggcgtcg cccagccggt cgacgcgcgc
     2101 catgccctcg ccgagcgccg cggcctgccg ctcgagctcg gagcggaggg aggcgacgtt
     2161 ctcgcgcacc gacgacacgg cgcgctcgac ctcggtcctg agctcctcgc cgatgcggac
     2221 gacgctcgcg gcggcgtcgc gctgatcgtc cccggcgctc gtgaccgcgg cggagatctc
     2281 ccgctccatc tccgcgagcc gctcggcgac gctcgagctc atctccgcgc gaacggcctc
     2341 gacg
//
LOCUS       DASZOB010000071         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_72250, whole genome shotgun sequence.
ACCESSION   DASZOB010000071 DASZOB010000000
VERSION     DASZOB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72250"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VG709_01135"
     CDS             <1..483
                     /locus_tag="VG709_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31714.1"
                     /translation="LDAGAASGAWVVGPRLVDASGDEAAPKHRFPPPLAWRAGVASGD
                     GWRAVPWIAGAAMLFTPGHTDLRFDPRIFMYGEDEELCWRVWRHGGRVVLAERARVVH
                     EGGSAALTRWSTRAIARRMVWNRARFVRWHAGWRGLVRFAADLARRRVAATSARRASA
                     "
     gene            complement(455..1450)
                     /locus_tag="VG709_01140"
     CDS             complement(455..1450)
                     /locus_tag="VG709_01140"
                     /inference="COORDINATES: protein motif:HMM:NF018669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31715.1"
                     /translation="MSSARFSRIGYGGTERSARGQDSSATPARAAPRPRRRPMRPRMI
                     FLHIPKTGGATLRSILLRQFPARAIHVQQHRPQMAGPATDGSRPLAPPPVPAPDARAR
                     TQFRAFEAMTEAQRARVLLVVGHYVYGVHEAFPGPSTYVTILRDPVERALSLFHYRRT
                     AQGLWMGLEEYLASGVDPELENGQTRAIAGAAATPVAGEPPTHDGMLEAAKRNLSREF
                     AAVCVTERFDDGVALLGREFGWRRIYYLPRNVSAGRPRTVDVSEEAVGLIRQRSRADI
                     ALHRFATDLFEDRVRRIPDLGARVERLRRQNSLYQTVPLAARAVERLKRTRAART"
     gene            1410..2273
                     /locus_tag="VG709_01145"
     CDS             1410..2273
                     /locus_tag="VG709_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31716.1"
                     /translation="MPPYPMRLKRADDTTTGAATERLSPKQGDLPKRERRAYDVVVAL
                     TVAEIRASRDITIGGLLKWMLEPLSYMFVYFVLVASILIRAQFGGAYPLFLLCALVPF
                     RFFTGAVNGSMSLIQRFSNVVANIPLPKVLLPLVHLMVEAANLLVAFLLFLPMVVIYD
                     TDVWPSILWLPVILVSLVLLTAGPTYLGTLFGLYYPDLRGVAQNLIRAAFFVSTGLVA
                     MKAVPGDELRLLIRANPLSGVFDSFRSVFLHGTTPDLFDLVYPPFVGLVLLTIALPLF
                     RAREARIPKEV"
     gene            2273..>2996
                     /locus_tag="VG709_01150"
     CDS             2273..>2996
                     /locus_tag="VG709_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC31717.1"
                     /translation="MTTAVSLRGVGVRFVLPRHSRLGKAPRLLGTSKQRIWGLRDVTL
                     DVERGEAVGFIGPNGAGKTTLLRTIAGIYRPDEGSVIVRGRVGPLLSLSAGLMPQLSG
                     WENIALIATLLGVPRKRVPEIAPRIGEFSGLEEFLDAEVRTYSSGMRARLGFAVAAFS
                     DPELLVVDEILTVGDEEFRQKSQEVIERFVREGRTVLVATHQVQTLIRMCTRAVYLDG
                     GRVVETGDPAGVVKRYREQHHVD"
BASE COUNT          373 a         1022 c         1091 g          510 t
ORIGIN      
        1 ctcgacgccg gggccgcctc gggcgcgtgg gtcgtcggcc cgcggttggt cgacgcctcc
       61 ggcgacgagg cggcgccgaa gcaccggttc ccgccgccgc ttgcgtggcg tgccggggtg
      121 gcgtcgggcg acgggtggcg cgcggtcccg tggatcgccg gcgccgcgat gctgttcacg
      181 cccggtcaca ccgacctgcg gttcgatccg aggatcttca tgtacggcga ggacgaggag
      241 ctgtgctggc gcgtgtggcg gcacggcgga agggtcgtgc tggccgagcg cgcgcgtgtc
      301 gtccatgagg gagggagcgc cgcgctgact cgctggtcga cgcgcgcgat cgcgagacgg
      361 atggtgtgga accgtgcgcg cttcgtccgc tggcacgcag ggtggcgggg cctcgttcgc
      421 ttcgccgcgg atctcgcgcg gcgcagggtc gccgctacgt ccgcgcggcg cgcgtccgct
      481 tgagccgctc gacggcccgc gcggcgaggg ggaccgtctg gtacagcgag ttctggcgcc
      541 gcagtcgctc gacccgtgcg ccgaggtccg ggatgcggcg gacgcggtcc tcgaacaggt
      601 cggtcgcgaa gcggtggagc gcgatgtcgg cacggctccg ttgccggatg aggcctacgg
      661 cctcctcgga cacgtccacg gtcctcggcc ggccggcgct cacgttccgc gggaggtagt
      721 agatgcgccg ccacccgaac tcgcgtccga gcagcgcgac gccgtcgtcg aagcgctcgg
      781 tcacgcacac cgcagcgaac tcgcgcgaga gattgcgctt cgcggcctcg agcatcccgt
      841 cgtgggtcgg tggttcgccg gctaccggtg tcgcggccgc gccggcgatc gctcgggtct
      901 gtccgttctc gagctccggg tccacgccgc tcgcgaggta ctcctccaga cccatccaca
      961 gcccctgcgc ggtccgtcgg tagtggaaca gcgacagggc ccgttccacg ggatcgcgca
     1021 ggatcgtcac gtacgtcgac ggtccgggga acgcctcgtg cacgccgtag acgtagtggc
     1081 cgaccaccag gaggacgcgg gctcgctgcg cctcggtcat cgcctcgaac gcgcggaact
     1141 gcgtccgggc gcgtgcgtcc ggcgcgggca cgggtggcgg ggcgagcgga cgggaaccgt
     1201 ccgtcgcggg accggccatc tggggccggt gttgctgcac gtggatcgcc ctcgcgggga
     1261 actggcggag gaggatcgag cggagcgtcg ctccgccggt cttggggatg tggaggaaga
     1321 tcatcctagg tctcattggt cgtcggcgtg gccgtggcgc ggcgcgcgcc ggagtcgcgc
     1381 tgctatcctg accgcgggct gatctctccg tgcccccata ccctatgcga ctgaagcgcg
     1441 cggacgacac gacgacgggg gccgcgacgg agcggctgtc tcccaaacaa ggcgacctcc
     1501 ccaagcgcga gcgtcgggca tacgacgtcg tcgtcgcgct gacggtcgcg gagatccgcg
     1561 cgagccgcga catcacgatc ggcgggctcc tcaagtggat gctggagccc ctgtcgtaca
     1621 tgttcgtgta cttcgtgctg gtcgcctcga tcctgatccg agcgcagttc ggcggcgcgt
     1681 atccactgtt cctgctgtgt gcgctcgtcc cgttccggtt cttcaccggc gccgtgaacg
     1741 ggtcgatgag cctgatccag cggttctcga acgtcgtcgc gaacatcccg ttgccgaagg
     1801 tgctccttcc gctcgtccac ctcatggtgg aggcggcgaa tctcctggtg gcgttcctgt
     1861 tgttcctccc gatggtcgtg atctacgaca ccgacgtctg gccgtcgatc ctgtggttgc
     1921 cggtgatcct cgtctcgctc gtgctgctga ccgccggccc gacgtacctc ggcacgctgt
     1981 tcgggctcta ctaccccgac cttcgcgggg tagcccagaa cctcatccgc gccgcgttct
     2041 tcgtctcgac cgggctcgtg gcgatgaagg cagtgccggg ggacgagctg cggctcctga
     2101 tccgcgcgaa ccccctgtcg ggcgtcttcg attcgttccg ctccgtgttc ctccacggca
     2161 cgacacccga tctgttcgac ctcgtgtatc cgcccttcgt cggcctcgta ctcctgacga
     2221 tcgcgcttcc gctgttccgg gcacgcgagg cgcgcatccc gaaggaggtc tgatgacgac
     2281 cgccgtctcc ctgcgcggcg tcggcgtgcg gttcgtcctc ccgcggcact cgcgtctggg
     2341 gaaggcgccg cggctcctcg ggacctcgaa gcagcggatc tggggactcc gcgacgtcac
     2401 gctcgacgtg gagcgcggag aggcggtcgg cttcatcggt ccgaacggcg ccgggaagac
     2461 cacgctgctc cggaccatcg ccggcatcta ccggcccgac gagggaagcg tcattgtccg
     2521 cggccgcgtg ggcccgctgc tgtcgctgtc cgcgggcctc atgccgcagc tctcggggtg
     2581 ggagaacatc gcgctgatcg cgacgctgct cggtgtgccg aggaagcggg tgcccgagat
     2641 cgcgccgcgc atcggggagt tctccgggct cgaggagttc ctcgacgccg aggtcaggac
     2701 ctactcgtcg gggatgcgcg cgcgtctggg gttcgcggtg gcggccttct ccgaccccga
     2761 gctcctcgtg gtggacgaga tcctgacggt cggcgacgag gagttccgcc agaagagcca
     2821 ggaggtgatc gagcgcttcg tccgcgaggg caggacggtg ctcgtcgcca cccaccaggt
     2881 gcagactctc atccggatgt gcacgcgcgc cgtctatctc gacggtggtc gcgtcgtcga
     2941 gacgggcgac ccggcgggcg tggtgaagcg gtatcgagag cagcaccacg tcgatc
//
LOCUS       DASZOB010000072         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_152869, whole genome shotgun sequence.
ACCESSION   DASZOB010000072 DASZOB010000000
VERSION     DASZOB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152869"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VG709_01155"
     CDS             <1..1135
                     /locus_tag="VG709_01155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC31718.1"
                     /translation="VERVGEDEGLRLARAAERAVGELEEFCEREGVDAHFHRGGWLWT
                     ATSKAQVGGWNGAVALAERYGERPFQVLSSDEVRARTGSPVHFGAAFERDVATVHPAL
                     LARGLRRVALARGVRIYERSPLVRLDRERGIAETPAGSVRAAAIVLALNAWALAVPEL
                     RRALVALSSDMVATEPIPDRLRESGWTGGEAVSNSRLLVHYYRTTRDGRIALGRGGGR
                     LAFGARVDSKFDHDERQTRELEAGLVELVPAARGARITHAWGGPIDRTADGLPFCGRL
                     SGKRHVVYGTGFSGNGVAPSLTIAKILASSALGRDDEWSGSGLNRGVPGRFPPEPVRF
                     LGGLVVRAAVRRKERREDAGKSVDPVTRRVAALAPSGFFRVSR"
     gene            complement(1379..1504)
                     /locus_tag="VG709_01160"
     CDS             complement(1379..1504)
                     /locus_tag="VG709_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31719.1"
                     /translation="MGRGSDVPKRRDATFADFSNYATLERQNDAIAAPGVYVRSA"
BASE COUNT          188 a          554 c          573 g          224 t
ORIGIN      
        1 cgtcgaacgc gtcggcgaag acgagggcct gcggctcgcc cgggcggccg agcgcgcggt
       61 cggggagctg gaggagttct gcgagcggga gggggtcgac gcccacttcc accgcggcgg
      121 ctggctgtgg acggccacgt cgaaggcgca ggtgggcggc tggaacggtg ccgtggcgct
      181 cgccgaaagg tacggtgagc ggccgtttca ggtgctgagc tccgacgagg tgcgcgcgcg
      241 gacgggatcg ccggtccact tcggcgcggc tttcgagcgc gacgtcgcga cggtgcaccc
      301 cgccctgctc gcgcgcggcc tccgccgcgt tgcgctcgcg cgcggcgtcc gtatctacga
      361 acgctcgccg ctcgtacggc tcgaccgcga gcgcgggatc gccgagacgc cggcgggctc
      421 ggtccgggcc gccgcgatcg tcctggccct gaacgcctgg gcgctcgccg tccccgagct
      481 gcgccgggcg ctcgtcgcgc tctcgagcga catggtcgcg accgagccga ttcccgaccg
      541 cctacgagag agcgggtgga ccgggggaga ggcagtctcg aactcccgtc tgctcgtcca
      601 ctactaccgg acgacgaggg acggccggat cgcgctcggg cgcggcggcg ggcgcctcgc
      661 cttcggcgcg cgggtcgact cgaagttcga ccacgacgag cggcagacgc gtgagctcga
      721 ggcggggctc gtcgagctcg tcccggccgc aagaggcgcg aggatcacgc acgcctgggg
      781 tggcccgatc gaccgcaccg cggacggcct tcctttctgc ggccggctct ccggcaagcg
      841 gcacgtcgtc tacggaacgg ggttctccgg gaacggcgtc gcgccgtcgc tcaccatcgc
      901 caagatcctc gcctcgagcg ccctcgggcg cgacgacgag tggtcgggct ccgggctgaa
      961 ccgcggcgtg ccggggcggt tcccccccga accggttcgc ttcctcggcg ggctcgtcgt
     1021 ccgcgcggcg gtacgccgga aggagcgccg cgaagacgcg ggcaagagcg tcgacccggt
     1081 gacgcgccgc gtcgccgctc tcgccccgtc aggcttcttc cgcgtgtcgc gctagccgct
     1141 actacccgcc ggcgcggatc aggtagccga agtaccgacc cgcgacggcg cggagcggat
     1201 gtccttggaa cccgtacgcg gcgttcgcgg tgtttgtatg tcgccgcgtc gtcgcggagc
     1261 ttttgcatca ttgtccgggg cgtccccgtg cacgcgcccg ttgagatgca gagcgccgcg
     1321 gtcccggcct atgcggtgag cgatgcgtgt ccccgagatc gtcgtgtagc cgccgccttc
     1381 acgccgacct gacatacacg ccgggcgcag cgatcgcgtc gttctgtcgc tcgagcgtcg
     1441 cgtagttcga gaagtccgcg aacgtggcgt cacgtcgctt cggcacgtcg gagccgcgcc
     1501 ccacccggca gcccgttgaa gtcggcgccc gccgtcacc
//
LOCUS       DASZOB010000073         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_82264, whole genome shotgun sequence.
ACCESSION   DASZOB010000073 DASZOB010000000
VERSION     DASZOB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82264"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VG709_01165"
     CDS             <1..595
                     /locus_tag="VG709_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC31720.1"
                     /translation="IDYIAPGLVATAAMYGTSFEVTFNCFVKMQFGKVYDAVMATPLS
                     IEDVGLGELLWGTTRALIYGVAFLLIASLFGVVHTPLVMLAPLAIALIGLMFSVIGLA
                     FTAVIPVIDYFTYYWTLFMTPMFLFSGIFFPLETMPAWVQALAWFMPLHHAVNLMRAL
                     VLTGDVGAAASAAAWVGGLTAALFVLPLNLLRRRLVK"
     gene            677..1444
                     /locus_tag="VG709_01170"
     CDS             677..1444
                     /locus_tag="VG709_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWC31721.1"
                     /translation="MNVIALAKWVPNPAGTPEMGPDNRLKRQGAEGALDPGDEPSIEQ
                     ALRLVEQSEGEVTVVSMGPEVATEAIRKALSMGCHKGIQITDEALAGADILVTARVLA
                     AAIKRQEFDLVLTAVESTDGYTGTLPQTLAEFLGVPSLAFAKKVELTDGKLRVERQTE
                     TGYEVVECELPAVVAVTAGATEPRYPTLKGIMQAKQKPLEKVAVSDLGLSEDDTKPTQ
                     EVVGLESAPERGAGEIVEDADEATQKIVSLLKEAKVI"
BASE COUNT          264 a          524 c          555 g          249 t
ORIGIN      
        1 catcgactac atcgcgccgg gcctcgtcgc gaccgccgcg atgtacggga catcgttcga
       61 ggtcacgttc aactgcttcg tgaagatgca gttcggcaag gtctacgacg ccgtgatggc
      121 gacgccgctg tcgatcgagg acgtcgggct cggcgagctc ctctggggga cgacccgcgc
      181 gctgatctac ggggtggcgt tcctcctgat cgcatcgctg ttcggcgtcg tccacacgcc
      241 gctcgtgatg ctcgcgccgc tcgcgatcgc gctgatcggg ctgatgttct cggtcatcgg
      301 gctcgccttc acggcggtca tcccggtgat cgactatttc acctactact ggaccctgtt
      361 catgaccccg atgttcctgt tctcggggat cttcttcccg ctcgagacga tgccggcgtg
      421 ggtgcaggcg ctcgcgtggt tcatgccgct ccaccacgcc gtgaacctca tgcgcgcgct
      481 cgtgctgacc ggcgacgtcg gagccgccgc gtcggcggcc gcgtgggtgg gcggcctgac
      541 cgccgcgctc ttcgtccttc cgctcaacct cctacgtcga cggctcgtga agtagcggtg
      601 tcgcgccggt cgggggtcgc cggaatcggg tagaaatagc gccgcatcac ccgccgattc
      661 caggaggcaa cgaaccgtga acgtcatcgc gctcgcaaag tgggtgccga atccggcggg
      721 gacgccggag atgggccccg acaacaggct caaacgccag ggcgccgagg gggcgctcga
      781 tcccggagac gagccgtcga tcgagcaggc cctgcggctc gtcgagcagt ccgagggcga
      841 ggtcaccgtc gtatcgatgg gaccggaggt cgccaccgag gcgatccgca aggcgctctc
      901 gatgggatgc cacaagggca tccagatcac cgatgaggcc ctcgccgggg ccgacatcct
      961 cgtgacggcg cgcgtgctcg ccgccgcgat caagcggcag gagttcgacc tcgtccttac
     1021 ggccgtcgaa tccacggacg gatacaccgg cacgctgccg cagacgctcg cggagttcct
     1081 cggcgtgccg agcctcgcgt tcgcgaagaa ggtcgagctc acggacggga agctccgcgt
     1141 cgaacggcag accgagaccg ggtacgaggt cgtcgaatgc gaactccccg cggtcgtcgc
     1201 cgtaacggcc ggcgcaacgg agccgaggta cccgacgctg aagggcatca tgcaggcgaa
     1261 gcagaagccg ctcgagaagg tagcggtgtc ggatctcggc ctttcggagg acgacacgaa
     1321 gccgacccaa gaggtcgtcg ggctcgagag cgcgccggag cgcggcgccg gagagatcgt
     1381 cgaggacgcc gatgaggcca cccagaagat cgtgtcgctc ctcaaggaag cgaaggtgat
     1441 ctgacatggc gaccgtatgg gtgtatgcgg agatagcccc cgacggtacg gtccacccga
     1501 gcgcgctcga gctcctcacg aaggcgcgcg agctcggcga cgccgttgag gcggtcgcgc
     1561 tcgggccagg cgcgacgcag gcggtcgaga cg
//
LOCUS       DASZOB010000074         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_193407, whole genome shotgun sequence.
ACCESSION   DASZOB010000074 DASZOB010000000
VERSION     DASZOB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193407"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1730)
                     /locus_tag="VG709_01175"
     CDS             complement(162..1730)
                     /locus_tag="VG709_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC31722.1"
                     /translation="MDYKGRLFGRSVLEATFRSFYGTDLFLGELLVAEDAERARRFLF
                     RVIEVRYGSDSSDPHWAERTAGAYMAGDVAGASYELHDPEQRLYKIATCAPLGYIDAG
                     GEFRKPKSLPAQFARVTAPTVGDVAFLRERMGDLRVGLLRSGEDTIELEVAIRGETLT
                     SHVGVFATTGMGKSNLMKVLAGSILESQGRYALLLFDPHGEYLDGGGGIRRGLRDHPW
                     AADRLRVYATRPGAAAGHTALRLSLAELTLGDLQTAYEWSGPQLEAMYKLQRFYREDW
                     LPTVADEGEDVEELAEQIGAAASTIQVIRRRAQRILDLECISRDVSQASLSERIVREL
                     SEGYSVLVETSGVSEMEEILVASVIARRLLDRYSTAYLDDRRAFEALPPTLVALEEAQ
                     RVLSRVKRSEENVFPRIAREGRKFRVGLCAVSQQPKLIDVELLSQFNTFFVLGLADEK
                     DRNILKGSSKQDISDLGPEIQTLMPGEAIVTNLEAPFALPAKVHLYEEYLKSVKGPAP
                     LPPPPERASSGFAE"
     gene            complement(1732..>2041)
                     /locus_tag="VG709_01180"
     CDS             complement(1732..>2041)
                     /locus_tag="VG709_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HWC31723.1"
                     /translation="LYVGDIFVAHLAPTAAHAFRVDVARATGAADDVLGRLAGCSRDP
                     SFVGYPYPLARVHRAARVSGYAVADLRRGFRAALGTRGLSEDDAEVLFQDFHEVLNRS
                     "
BASE COUNT          312 a          719 c          714 g          296 t
ORIGIN      
        1 caggaacgcg tccggctcgg tcgcgatcgt ctcgttgatc gcccagtcga acgaacgcag
       61 gaagtcctcc tcgcggacgt tccggccgtc gtcgtcacgg aggtgtgcga ggtggctccg
      121 gccgaggtgc gcgtcgccga gcgcgatgag ccgcgccacg gctactccgc gaacccgctc
      181 gaggcgcgct ccggaggcgg cggaagggga gcggggcctt tcacggactt caggtactcc
      241 tcgtagaggt gaaccttggc cggcagcgcg aacggcgcct cgaggttcgt cacgatggcc
      301 tcgccgggca tcagcgtctg gatctcgggt ccgagatcgg agatgtcctg cttcgacgag
      361 cccttgagga tgttccggtc cttctcgtcg gcgagcccga gcacgaagaa cgtgttgaac
      421 tgcgagagca gctcgacgtc gatcagcttc ggttgctggc tcaccgcaca gagcccaacg
      481 cggaacttcc gaccctcgcg cgcgatccgc gggaagacgt tctcctccga ccgcttcaca
      541 cgcgagagca cgcgctgggc ctcctcgagc gccacgagcg tgggcggcag tgcctcgaag
      601 gctcgtcggt cgtccaggta ggcggtcgag tagcggtcga ggagccgcct cgcgatcacc
      661 gacgcgacga ggatctcctc catctccgag acgcccgagg tctccacgag caccgagtag
      721 ccctcggata gctcgcgcac gatccgctcg gagagcgacg cctgggagac atcgcggctg
      781 atgcactcga ggtcgaggat ccgctgcgcg cgccgccgga tgacctgaat cgtcgatgcg
      841 gcggcgccga tctgctcggc gagctcttcg acgtcctccc cttcgtctgc aaccgtcgga
      901 agccagtcct cccggtagaa ccgctgaagc ttgtacatcg cctcgagctg cggcccgctc
      961 cattcgtacg cggtctggag gtcgccgagc gtgagctccg cgagcgagag gcgcagcgcc
     1021 gtatgcccgg ccgcggcgcc cgggcgcgtc gcgtagacgc ggaggcggtc ggcggcccac
     1081 gggtggtcgc ggagcccgcg ccggatccct ccaccgccgt cgaggtactc gccgtgggga
     1141 tcgaacagga gcagcgcgta ccggccctgc gactcgagga tcgacccggc cagcaccttc
     1201 atgaggttcg acttccccat cccggtcgtc gcgaagacgc cgacgtggga cgtgagcgtc
     1261 tcgccgcgga tcgcgacctc gagctcgatc gtgtcctcgc ccgagcgcag cagcccgacg
     1321 cggaggtcgc ccatccgctc gcgcaggaac gcgacgtccc cgacggtcgg cgccgtgacc
     1381 cgcgcgaact gcgccggaag cgacttcggc ttgcggaact cgccgcccgc gtcgatgtag
     1441 ccgagcggcg cgcaggtggc gatcttgtag aggcgctgct cggggtcgtg gagctcgtac
     1501 gacgcgccgg cgacgtcgcc ggccatgtac gcgccggccg tccgttccgc ccagtgcggg
     1561 tccgacgaat cggagccgta gcgcacctcg atcacacgga acaggaaccg gcgtgcccgc
     1621 tcggcgtcct ccgcgaccag cagctctccg aggaacaggt ccgtcccgta gaacgaccgg
     1681 aacgtcgcct cgaggacgct gcggccgaag agtcgtccct tgtagtccat ctcagctccg
     1741 gttcagcacc tcgtggaaat cctggaagag cacctcggcg tcgtcttcgg acagaccgcg
     1801 ggtcccaagc gcggcgcgga acccccgccg caggtcggcg accgcgtagc cgctgacgcg
     1861 cgcggcgcgg tgtacccgcg cgagcgggta cgggtacccg acgaacgagg ggtcgcgcga
     1921 gcaccccgcg aggcgcccga gcacgtcgtc ggccgctccc gtcgctcggg cgacgtcgac
     1981 gcggaacgcg tgcgcggcgg tcggggcgag gtgcgcgacg aagatgtccc cgacatagag
     2041 c
//
LOCUS       DASZOB010000075         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_92420, whole genome shotgun sequence.
ACCESSION   DASZOB010000075 DASZOB010000000
VERSION     DASZOB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92420"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VG709_01185"
     CDS             <1..660
                     /locus_tag="VG709_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31724.1"
                     /translation="RVVHLITRAQGGLHDYQGFLHEAGHALHYAGCDPALAYTFRRLS
                     RDHALTEIYSFLLESVTREAGWHAEHFDLDMDEAEDRAEGTRFLEVLLFRRYAAKLEY
                     ELELWSDFANAPAYSGVYADRLRAAIGFRYRPDGYLADMDDGFYSADYLRAWIRTAQM
                     RAYLRERVGGDWWERPETGDLLRDLFREGTAPANEEIAVRLGYDPLDTEPLVDELVAT
                     P"
     gene            complement(606..1466)
                     /locus_tag="VG709_01190"
     CDS             complement(606..1466)
                     /locus_tag="VG709_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HWC31725.1"
                     /translation="MIAEVRVESWAELQERLFDEAWKEELGRFRSDFAFRGHANAAGD
                     LVTSLARLDGDYAELENHLLRNFRKYARRDAVSVDSPWEWLALAKHHHLPTRVLDWSY
                     SPYVALHFATVDARRAESEAAEAAVWCVDFVRVREALPDALVNVLADERSNVFTTEML
                     SGTAESLAELDELREEDDFVVFFEPPSLDERIVNEYALFSLMSSATARLDEWLAERPD
                     LARRIVLPAELKWEVRDKLDQANVTERVLFPGLDGLSAWLTRHYSPARRAVYGVATSS
                     STSGSVSSGS"
BASE COUNT          235 a          563 c          569 g          239 t
ORIGIN      
        1 cgcgtcgtcc acctgatcac gcgggcgcag ggcgggctcc acgactacca gggattcctc
       61 cacgaggcgg gccacgcgct ccactacgcg gggtgcgatc ccgcgctcgc ctacaccttc
      121 cgccgcctct cccgcgacca cgccctcacg gagatctact cctttctcct cgagtcggtc
      181 acgcgtgagg ccggctggca cgcggagcac ttcgacctcg acatggacga ggcggaggac
      241 cgcgcggagg ggacgcgctt cctcgaggtc ctcctctttc gccgctacgc cgcgaagctc
      301 gagtacgagc tcgagctctg gtcggatttc gcgaacgcgc ccgcgtactc cggcgtctac
      361 gcggaccgcc tgcgcgcggc gatcggcttt cgctaccgcc cggacggcta cctggccgac
      421 atggacgacg gcttctactc ggccgactac ctgcgcgcat ggatcaggac cgcgcagatg
      481 cgcgcgtacc tgcgcgagcg cgtcgggggc gactggtggg agcggcccga gaccggcgac
      541 ctcctgcgcg atctcttccg tgagggaacc gcgcccgcga acgaggagat cgccgtgcgg
      601 ctcggctacg acccgctcga caccgagccg ctcgtcgacg agctcgtcgc cacgccgtag
      661 acggcgcggc gcgccgggct gtagtggcgt gtcagccagg cgctgaggcc gtccaggccc
      721 gggaagagga cgcgctccgt cacgttcgcc tggtcgagct tgtcgcgaac ctcccacttg
      781 agctcggccg ggagaacgat tcgccgcgcc aggtcgggcc gctcggcgag ccactcgtcg
      841 agtcgcgcgg tcgcgctcga catgagcgag aagagcgcgt attcgttcac gattcgctcg
      901 tccagggacg ggggctcgaa gaacacgacg aagtcgtcct cctcgcggag ttcgtcgagc
      961 tcggcgagcg attccgccgt gccgctcagc atctcggtcg tgaagacgtt cgagcgctcg
     1021 tcggcgagga cgttcacgag cgcgtccggg agcgcctcgc ggacgcgcac gaagtcgacg
     1081 caccagacgg cggcctcggc cgcctccgac tcggcgcggc gggcgtccac ggtcgcgaag
     1141 tggagcgcga cgtacggcga gtagctccag tcgaggacgc gtgtggggag atggtggtgt
     1201 ttggcgagcg cgagccattc ccacggcgag tcgaccgaga cggcgtctcg gcgcgcgtac
     1261 ttgcgaaagt tgcggaggag gtggttctcg agctccgcgt agtcgccgtc gaggcgcgcg
     1321 aggctcgtga cgaggtcgcc ggccgcgttc gcgtgcccgc ggaaggcgaa gtcggagcgg
     1381 aagcggccga gctcctcctt ccacgcctcg tcgaagagcc gctcctggag ctcggcccag
     1441 ctctcgacgc ggacctcggc gatcacgggc gggttcttgc cccgacccgc gcgtcttacg
     1501 catcgagtcc atgacgcatc gaatccgtga cgcttccggc gcaatccgcg cgccacgcgt
     1561 gcgcgcaagg gcggtacgct gaccggaggg ggaaccgcaa ccgggg
//
LOCUS       DASZOB010000076         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_1688,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000076 DASZOB010000000
VERSION     DASZOB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VG709_01195"
     CDS             <1..1193
                     /locus_tag="VG709_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWC31726.1"
                     /translation="VRLTIDADLQRTAEEALEYGIRIAHEDGEWAADGGALVAMDPRT
                     GEILALASHPTFDPRVFVGRVQQKDLRKLADRRANHPTLNRAVSGLYPPGSTFKPVTA
                     LAALEEGLLQPDEMIQCTGKEVIDGQTFMNWDPYVNEPMKLTTALAASCDTYFYDVGL
                     RFYEREDSPLQKWARRMGFERRTGVDIGPEERGLMPTPEWGRRYFDNEVDQLWTSGES
                     VQLAIGQGDVLVTPLQMTRFFALLANGGKLVEPHIVKAVEEPGNEGEPPVVLRPYSPK
                     PPTDVGLDPMALRVVQEGLYDAAHTNYGTSSGIFGSFPIPIAGKTGTAEKFVRLPGFQ
                     GLRDQAWWCGYGPYSKPELAVCALIENGGHGGTAAAPAALRVFEEYFNVEPESYTATI
                     VESD"
BASE COUNT          241 a          551 c          518 g          221 t
ORIGIN      
        1 acgtccggct aacgatcgac gccgacctcc agcggacggc cgaggaggcg ctcgagtacg
       61 gcatccgcat cgcgcacgag gacggcgagt gggcggccga cggcggggcg ctcgtcgcga
      121 tggacccgcg cacgggcgag atcctcgcgc tcgcgtcgca cccgacgttc gacccgcgcg
      181 tcttcgtcgg gcgcgtgcag cagaaggacc tgcgcaagct cgcggaccgc cgtgcgaacc
      241 acccgacgct gaaccgcgcc gtctcggggc tctacccgcc cgggtcgacg ttcaagccgg
      301 tgaccgcgct cgccgcgctc gaagagggac tcctgcaacc cgacgagatg atccagtgca
      361 cgggcaagga ggtcatcgac ggccagacgt tcatgaactg ggacccctac gtgaacgagc
      421 cgatgaagct gacgacggcg ctcgcggcgt catgcgacac gtacttctac gacgtcgggc
      481 tccgcttcta cgagcgcgag gactcgccac tccagaagtg ggcgcgccgg atgggtttcg
      541 agcgacggac gggcgtcgac atcggccccg aggagcgcgg cctcatgccg acgccggagt
      601 ggggccgccg ctacttcgac aacgaggtcg accagctctg gacgagcggc gagtcggtcc
      661 agctcgcgat cgggcagggg gacgtgctcg tgacaccgct ccagatgacg cgcttcttcg
      721 cgctgctcgc gaacggcggc aagctcgtcg agccgcacat cgtcaaggcc gtcgaggagc
      781 ccgggaacga gggcgagccg cccgtcgtcc tgcggccgta ctcgccgaag ccgccgaccg
      841 acgtcgggct cgatccgatg gcgctgcgcg tcgtccagga gggtctgtac gacgcggcgc
      901 acaccaacta cgggacctcg tccggcatct tcggctcctt cccgatcccg atcgccggca
      961 agacggggac ggcggagaag ttcgtacgcc tgcccggctt ccagggcctg cgcgaccagg
     1021 cttggtggtg tggctacggg ccgtactcga agcccgagct cgccgtctgc gcgctcatcg
     1081 agaacggcgg ccacggcggg acggccgccg cgccggccgc gctgcgcgtg ttcgaggagt
     1141 acttcaacgt cgagccggag tcgtacaccg cgacgatcgt cgaatccgac taagtgcccc
     1201 accgagcaac atccctcgtg cttctgggcg gctgcaaagc gtcgccaggc ggcgtcctcg
     1261 gtcgcccacg tactgcagtc agtacgcagg cttcctccgt cctcgcctgg ctcgcttttc
     1321 gcgcgccaga agcacgccaa tgtcaccgga tgaggcacta atggccactc gagtcgagca
     1381 cgcagcccgc ccgcatgcgc cgtccgagat cggcgcgttc ctccgccacg tcgactacct
     1441 cctgctcgcc gcgctcgccg gcctgatcgc gtacggcctc tgggtgctcg cggcggtgac
     1501 gcgcaacgac gtccccggtg atccctcgta c
//
LOCUS       DASZOB010000077         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_10876, whole genome shotgun sequence.
ACCESSION   DASZOB010000077 DASZOB010000000
VERSION     DASZOB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10876"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VG709_01200"
     CDS             <1..924
                     /locus_tag="VG709_01200"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWC31727.1"
                     /translation="DAFRHEALFYSREDDFVRQTSRFIRGGLAADEPVLVVVSGRKIQ
                     RLRSELGTDADRVRFADMNDVGRNPARIIPAWRDFVDEHAHTGRPLRGIGEPIFSSRT
                     ASEMVECELHEALLNVAFDGGPAWWLVCPYDTTALRPAILEEARRNHPFLAQGDRHHA
                     SAVYTGVDRSARPFDRPLPEPRGEVEEMPFCVGDLHAVREVVARHAAAFGLDDDRTSD
                     FVLATDEIASNSIRHGGGSGRLRLWAEGDVLISEVRDAGRIVDPLVGRVRPIPTGEGG
                     VGLWLATQLCDLVQVRTFATGSVVRLHTARP"
     gene            complement(937..1602)
                     /locus_tag="VG709_01205"
     CDS             complement(937..1602)
                     /locus_tag="VG709_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWC31728.1"
                     /translation="MGSSPDVVWQPSLLESAEKPEIDHDFSRLVRIDLDEASWVDHAP
                     GWVRGSDEVFAQVLASRTWQQRSRRMYDQRVLEPRLTAPWIAASGDPLEPPLLEEIRR
                     VLSERYDREFDSVGFNLYRDGRDSVAWHGDRIPKEIEDPIVALVSLGEPRKFLLRPKG
                     GGGARTFLLGRGDLLVTGGRTQRDWEHSVPKVKEAGPRISLAYRHGMDPRAYGDKRVE
                     PES"
     gene            complement(1623..2351)
                     /locus_tag="VG709_01210"
     CDS             complement(1623..2351)
                     /locus_tag="VG709_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC31729.1"
                     /translation="MLERLWSRWNFRRMEECATLLRWLEPRRGERILDIGSGHGFNDR
                     DIASAGARVAAIDLDRDRLRYARTHNAVAGVRFLAVDAERLPFDDASFDKVVSFCVIE
                     HFRDDDAVLDEVARVLVPGGWLVLSADSLSNPELRDVERTAQARRYSVNNLYTIGSLR
                     EKLAARGFEVQRWRYILTSPFTLALVRLSWRLDELPEALLPAKVAGYVALSTLGRAAS
                     AVAEAVAGRRDTGLTLLVKARKRR"
     gene            complement(2443..>3467)
                     /locus_tag="VG709_01215"
     CDS             complement(2443..>3467)
                     /locus_tag="VG709_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31730.1"
                     /translation="THEAGTWAKMEFHPTDEQLSMLGLPSRRILQSREYPTPTLVTQD
                     GRFFDVPVAELEAAMAEKPPKDGGGKGGGGGGSSSGSSNPAVAAYGGTGCIGIRPGAW
                     LLLLDGGIGWCSMAHVYGSPGNYDISTAGHCGKTGDTATVIAALGNRGDATGVVLLDF
                     GTFAQSHDGGLGNDWALIDIKPEYQGLVTPTMCFWGGPRGMYTATGDVASASFSGNSL
                     IPNVSVNPNPRLAQAVLHYGHGAAIGTGGTPRAGGAIAWRASHFMFFGALAPGDSGSA
                     SNAAPGDGSALADFNEAAGINTHIWIDPLMRSGFGIMGGTRATQVSGTLANGQLAGYP
                     APVPLP"
BASE COUNT          499 a         1235 c         1228 g          505 t
ORIGIN      
        1 gatgcgttcc ggcacgaggc gctcttctac tcccgcgaag acgacttcgt ccggcagacg
       61 agcaggttca tccgcggcgg tctcgccgcc gacgagccgg tcctcgtcgt ggtgagcggg
      121 cggaagatcc agcggctccg gtcggaactc gggaccgacg cggaccgtgt ccgcttcgcc
      181 gacatgaacg acgtcggccg caaccccgcc cggatcatcc ccgcgtggcg agacttcgtc
      241 gacgagcacg cccatactgg ccgtccgcta cgggggatcg gagagccgat cttctccagc
      301 cgaaccgcgt ccgagatggt cgagtgcgag ctccacgagg cgctcctgaa cgtggccttc
      361 gacggcgggc cggcctggtg gctcgtctgc ccgtacgaca ccacggcgct ccgccccgcg
      421 atcctcgagg aggcacgccg caaccatccg ttcctcgcgc agggcgaccg ccaccacgcg
      481 agcgccgtct acaccggcgt cgaccggagc gcacgtccgt tcgaccgccc gctcccggag
      541 ccacgcgggg aggttgagga gatgcccttc tgcgtcggcg acctccatgc cgtacgcgag
      601 gtcgtcgcga gacacgccgc cgcgttcggt ctcgacgacg accgcacctc ggacttcgtg
      661 ctcgcgaccg acgagatcgc ctccaacagc atccggcacg gcggagggag cggccggctc
      721 cggctgtggg ccgaaggcga cgtgctgatc agcgaggtcc gcgacgccgg ccgcatcgtc
      781 gatccgctgg tcggccgtgt gcgtccgatc ccgacgggcg aaggcggcgt ggggctttgg
      841 ctggccacgc aactgtgcga cctcgtccag gtacgaacgt tcgcgaccgg ttctgtcgtg
      901 cggcttcaca cggcgcgccc ctgaggcgcg gcccgctcag ctctccggct cgacgcgctt
      961 gtccccgtag gcgcgcgggt ccatgccgtg gcggtacgcg aggctgatcc gtggcccggc
     1021 ctccttgacc ttggggacgc tgtgctccca gtcgcgctgc gtgcgtccgc cggtcacgag
     1081 gaggtctccg cggccgagca ggaacgtgcg cgcgccgccg ccgcccttcg gccgcaggag
     1141 gaacttgcgc ggctcgccga gggagacgag cgccacgatc gggtcctcga tctccttcgg
     1201 gatgcggtct ccgtgccacg cgacgctgtc gcgcccgtcg cggtagaggt tgaagccgac
     1261 cgagtcgaac tcgcgatcgt agcgctcgga taacacgcgt cggatctcct cgagcagggg
     1321 aggctcgagc gggtcgccgg acgccgcgat ccacggcgcc gtgagccgcg gctccaggac
     1381 gcgctggtcg tacatcctcc gcgagcgctg ctgccacgtt cggctggcga ggacctgcgc
     1441 gaagacctcg tcggagccgc ggacccaacc gggtgcgtga tcgacccacg acgcttcatc
     1501 gaggtcgatc cggacgagtc gcgagaagtc gtggtcgatt tccggcttct cggccgactc
     1561 gaggagcgat ggctgccaga cgacgtccgg ggaagagccc atcgccgcga ctctagcaac
     1621 gcctaccggc gtttccgcgc tttcacgagg agcgtcagac ccgtgtcgcg tcgcccggcg
     1681 acggcttcgg cgacggctga cgcggcgcgt ccgagcgtcg agagcgcgac gtatccggcg
     1741 accttcgccg ggagcagcgc ttccggcagc tcgtcgagac gccacgacaa ccgcacgagc
     1801 gcgagcgtga acggcgacgt gaggatgtag cgccaccgct ggacctcgaa gccgcgggcg
     1861 gcgagcttct cgcgcagcga gccgatcgtg tagaggttgt tgacgctgta cctgcgggcc
     1921 tgtgccgtcc gctcgacgtc gcggagctcg gggttcgaga ggctgtcggc ggagagcacg
     1981 agccacccgc ccggaacgag cacgcgggca acctcgtcga ggaccgcgtc gtcgtcgcgg
     2041 aagtgctcga tgacgcagaa gctgacgacc ttgtcgaacg acgcgtcgtc gaacggaagc
     2101 cgctccgcgt ccacggcaag gaaccgaacg cccgcgacgg cgttgtgggt gcgcgcgtac
     2161 ctcaggcggt cgcggtcgag gtcgatcgcg gcgacccggg cgccggcact cgcgatgtcc
     2221 cggtcgttga acccgtggcc tgatccgata tcgaggatgc gctcaccgcg acgcggctcg
     2281 agccaccgaa ggagcgtcgc gcattcctcc atgcggcgga agttccaccg gctccagagc
     2341 cgttcgagca tccgcggacg gtacggggaa gccgtggccg gcgtcaaaga agacgagggg
     2401 cccggcaccc agccggggcc ctccgtcgcc cgggcggcga agctacggga gcgggaccgg
     2461 ggcgggatac ccggcgagct gtccgttcgc gagcgtcccg ctcacctgcg tcgcccgcgt
     2521 gccgcccatg atcccgaagc cgctccgcat gagggggtcg atccagatgt gggtgttgat
     2581 gccggccgcc tcgttgaagt ccgcgagcgc gctcccgtcg ccgggcgccg cgttcgacgc
     2641 ggatccggag tcgcccggcg cgagggcacc gaagaacatg aagtgcgagg cgcgccacgc
     2701 gatcgcccca ccggctcgcg gcgtcccgcc ggtcccgatc gcggcgccgt gcccgtagtg
     2761 caggacggcc tgcgcgagcc gcgggttcgg gttcaccgag acgttgggga tgaggctgtt
     2821 gccggagaag ctggccgacg ccacgtcgcc ggtcgcggtg tacatcccgc gtggcccgcc
     2881 ccagaagcac atcgtcggtg tgacgaggcc ctggtactcg ggcttgatgt cgatcagcgc
     2941 ccagtcgttc ccgaggccgc cgtcgtgtga ctgcgcgaac gtcccgaagt cgagcagcac
     3001 cacacccgtc gcgtcgcccc ggtttccgag cgccgcgatg acggtcgccg tgtcgccggt
     3061 cttcccgcag tggccggcgg tgctgatgtc gtagttcccc ggcgacccat agacgtgggc
     3121 catcgagcac cagccgatgc cgccgtcgag gagcagcagc caagcacccg gacggatgcc
     3181 gatgcagccg gtgccgccgt acgccgccac ggcggggttg gaggagccgc tactactccc
     3241 cccaccgccg ccccccttgc cgccgccatc cttcggcggc ttctcggcca tcgcggcctc
     3301 gagctcggcg acggggacgt cgaagaagcg gccgtcctgt gtcacgagcg tcggtgtcgg
     3361 gtattcgcgc gactgcagga tccgacgcga cgggagcccg agcatcgaaa gctgctcgtc
     3421 ggtcgggtgg aactccatct tcgcccacgt ccccgcctcg tgggtcg
//
LOCUS       DASZOB010000078         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51911, whole genome shotgun sequence.
ACCESSION   DASZOB010000078 DASZOB010000000
VERSION     DASZOB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51911"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VG709_01220"
     CDS             complement(<1..114)
                     /locus_tag="VG709_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWC31731.1"
                     /translation="MTYLSRAKTLGDVLVVGLNSDASVARLKGPGRPINTLE"
     gene            complement(60..1214)
                     /locus_tag="VG709_01225"
     CDS             complement(60..1214)
                     /locus_tag="VG709_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HWC31732.1"
                     /translation="MSAELSRVVDSFERLQVAVIGDAMLDTYMHGTADRLSREAPVPI
                     VTLEGAVDVPGGAANTAVNAAELGASVRLLTVVGEDDEGALLRKELERLRVGAEDVIA
                     DPSRWTLAKRRISAQGQQLLRFDRGSTAPVEGDAEDELVERVRSVVPRCDAIIVSDYG
                     YGVVTRRVVDAIVEARREGAVVVVDAKDLRRYRRVHATAVKPNYEEAVRLLGEHEVRG
                     LDARAEQLTDRGDRLLELTGADAAAVTLDTEGALLFEHGRPPYRTFARHTRLARPAGA
                     GDTFVATLALAFAAGATSTAAAELASAASGVVIEKDGTSACAGSSSASTSPPRRSGSL
                     TRNGWWPGWSSTAGRTTASCSRTAASTSCTAATSRISRARRRSATSSSSG"
     gene            complement(1211..2179)
                     /locus_tag="VG709_01230"
     CDS             complement(1211..2179)
                     /locus_tag="VG709_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC31733.1"
                     /translation="MSGLRVFTWHVHGNYLWYLSRAPHEFFVPVKPGRPERYVGRGTT
                     FPFARNVHEIAAEEVRRTSFDVVLFQARDNWVHDQREILTPEQRRLPRIYLEHDPPRE
                     HPTDTRHVVDDPDVLLVHVTPFNDLMWDSGRTPTRVIEHGVVVPEDVRYTGELDRGIA
                     VVNNLPRRGRRVGADLFERARRRVPLDLVGMGSQDAGGLGDVRPPELPEFEARYRFFF
                     HPIRYTSLGLAACEAMMIGLPVVAPATTEMTTVIEDGVSGFVDTNVERLIEGMETLLA
                     EPGLAARLGAAARARALERFHIDRFIGDWNTAFADVTGSSRRGRVA"
     gene            complement(2219..3238)
                     /locus_tag="VG709_01235"
     CDS             complement(2219..3238)
                     /locus_tag="VG709_01235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWC31734.1"
                     /translation="MTDPLPEEVRSVAVLRALPGLGDLLCAVPALRALRRAFREARIT
                     LIGLDPEARWFVERFRYLDALMALPGYPGLPERAADPNEVVEFLAAAQRRRFDLAVQL
                     HGSGEITNHVIALLGAGAAAGAFVEGRYRPDERRFIEYRDDEPEVVRNVRIVERLGVP
                     PAGLELEFPVHGADEAELERVLGDARPRPGSYAVVHPGASIRDRRWPADRFAEVADVL
                     ARHGLEIFVTGGEVERDVAREVRGAMSTRDAVDLSGRTSVGAVAALARDARVVVCNDT
                     GISHLAAALRVPSVVVFTSESDRWAPLDRNLHRLVRDPGGVPVAAVLDELAALLGTPA
                     GSGPR"
BASE COUNT          495 a         1227 c         1162 g          460 t
ORIGIN      
        1 ctcgagcgtg ttgatcgggc ggccgggccc cttcagccgc gcgacgctcg cgtccgagtt
       61 cagcccgacg acgaggacgt cgccgagcgt cttcgcgcgc gagagatacg tgatgtggcc
      121 gcggtgcagg atgtcgaagc agccgttcgt gaacacgacg cggtggtcct gccggcggtg
      181 gaactccagc cgggccacca gccgttccgg gtcaacgagc cgcttctcct cggcggcgaa
      241 gtgctcgcgg agctcgagcc cgcgcatgcc gaggtcccgt ccttctcgat cacgacgcca
      301 gacgcggccg acgcgagctc cgccgcggcc gtcgacgtcg caccggccgc aaacgcgagc
      361 gcgagcgttg ccacgaacgt atcgccggct ccggcggggc gcgcgagcct cgtgtggcgg
      421 gcgaacgtgc ggtacggcgg gcgcccgtgc tcgaacagca gcgccccctc ggtgtcgagt
      481 gtgacggcgg cggcgtcggc accggtgagc tcgagcagcc gatcgccccg atccgtgagc
      541 tgctcggccc tggcgtcgag cccgcgcacc tcgtgctcgc cgaggagccg caccgcctcc
      601 tcgtagttcg gcttcaccgc cgtcgcgtga acacgtcgat aacgcctgag gtccttcgcg
      661 tccaccacga cgaccgcacc ctcgcgccga gcctcgacga tcgcgtcgac cacacggcgc
      721 gtcacgacgc cgtacccgta gtccgagacg atgatggcgt cgcagcgagg caccaccgac
      781 cggactcgct cgacgagctc gtcttcggca tccccctcga cgggcgccgt cgaacccctg
      841 tcgaagcgaa ggagctgctg gccctgcgcg gagatccgac gcttcgccag cgtccagcgc
      901 gacggatccg cgatcacgtc ttccgcgccg acccgcaagc gttccagctc cttgcggagc
      961 agcgcgccct cgtcatcctc gccgacgacc gtcaggaggc gcacgctcgc tccgagctct
     1021 gcggcgttca ccgcggtgtt cgcggcgccc cccggaacgt ccacagctcc ctcgagcgtg
     1081 acgatcggga ccggtgcctc gcgggagaga cgatcggccg tcccgtgcat gtacgtgtcg
     1141 agcatcgcgt cgccgatgac cgcgacctgg aggcgctcga acgagtcgac gacccgcgag
     1201 agctcggcgc tcatgcgaca cgcccccgac gcgacgatcc cgtcacgtcc gcgaacgcgg
     1261 tgttccagtc gccgatgaag cgatcgatgt ggaaccgttc cagcgcgcgc gctcgcgccg
     1321 cagcgccgag gcgcgccgcg agcccgggtt ccgcgagcag agtctccatc ccctcgatga
     1381 ggcgctcgac gttcgtgtcg acgaacccgg agaccccgtc ctcgatgacc gtggtcatct
     1441 cggtcgtcgc cggggcgacg accggcaacc cgatcatcat cgcctcgcat gccgcgagcc
     1501 cgaggctcgt gtaccggatc ggatggaaga agaaccggta ccgggcctcg aactccggta
     1561 gctcgggggg ccggacgtcg ccgaggccgc cggcgtcctg cgaccccatc ccgacgagat
     1621 cgagcggcac ccggcgccgc gctcgctcga agaggtcggc gcctacgcgg cgaccccgcc
     1681 gaggcaggtt gttcacgacc gcgatgccgc ggtcgagctc gccggtgtag cgcacgtcct
     1741 ccgggacgac gacgccgtgc tcgatcacgc gcgtgggggt ccggccgctg tcccacatga
     1801 ggtcgttgaa cggcgtcacg tgcacgagca gcacgtcggg gtcgtcgacg acgtgccggg
     1861 tgtcggtcgg gtgttcgcgg ggcggatcgt gttcgaggta gatgcgcggc agcctccgct
     1921 gctccggcgt gaggatctcg cgctggtcat ggacccagtt gtcccgcgcc tggaacagca
     1981 cgacgtcgaa cgacgtacgc ctgacttcct ccgcggcgat ctcgtgtacg ttgcgcgcga
     2041 acgggaacgt cgtcccgcgc ccgacgtatc gctcgggacg ccccggcttc accgggacga
     2101 agaactcgtg cggcgcacgt gacaggtacc acagatagtt gccgtggacg tgccacgtga
     2161 acacccgcag gccggacatc tcggagcggt tcttcccgag ccgctcctcg gtaaacggct
     2221 agcggggacc gctgcccgcc ggcgtgccga ggagcgcggc gagctcgtcg agcaccgcgg
     2281 cgaccggaac gccgcccggg tcccggacga ggcggtgaag atttcgatcg agaggcgccc
     2341 accggtcgga ctccgacgtg aacacgacga cgctcgggac gcgaagcgcg gccgcaagat
     2401 gcgagatgcc cgtgtcgttg catacgacga cgcgcgcgtc tcgcgccaga gccgcgaccg
     2461 ccccgacgct cgtccggccg gagaggtcga cggcatcccg cgtcgacatc gcgccgcgga
     2521 cctcgcgcgc gacgtctcgc tcgacctcgc ccccggtgac gaagatctcg agcccgtgcc
     2581 gcgcaaggac gtcagcgacc tcggcgaacc ggtccgccgg ccaccggcgg tcccggatgc
     2641 tcgcgccggg gtgaacgacc gcatacgacc cgggacgcgg acgcgcgtct ccgaggacgc
     2701 gctccagctc cgcctcgtcg gcgccgtgga ccgggaactc gagctcgagc cccgcgggcg
     2761 gcacgccgag gcgctcgacg atgcggacgt tccgcacgac ctccggttcg tcgtcgcggt
     2821 actcgatgaa ccggcgttcg tcggggcggt agcgtccctc gacgaaggcg ccggcggccg
     2881 cgccggcgcc gaggagcgcg atgacgtggt tcgtgatctc cccgctccca tggagctgga
     2941 ccgcgagatc gaagcggcgc cgctgcgcgg cagcgaggaa ctcgacgacc tcgttggggt
     3001 cggctgcccg ctcggggagg ccggggtatc cgggtaacgc catcagggcg tcgaggtacc
     3061 ggaaacgttc gacgaaccac ctcgcctcgg ggtcgagccc gatcagcgtg atccgggcct
     3121 cccggaaggc tcgcctcagc gcgcgaagtg ccggcacggc gcacagcagg tcgccgaggc
     3181 cggggagcgc gcgaagcacc gcgacgctcc gcacttcttc gggcagcgga tccgtcactc
     3241 gaggagctcc tcctcctcga cgacgtcgag cgcgagcgtt cggtacccga cctcctcgaa
     3301 gagcaccgtg atgcggccgt cgtccacgcg ctgcaccgtt cctc
//
LOCUS       DASZOB010000079         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_102642, whole genome shotgun sequence.
ACCESSION   DASZOB010000079 DASZOB010000000
VERSION     DASZOB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102642"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VG709_01240"
     CDS             <1..441
                     /locus_tag="VG709_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWC31735.1"
                     /translation="LVPPAATTAGPPVWLGAQADEVVRMAARVADGWNGWGMDVEAFR
                     AKATLLDREARAAGRTAAATWAGIVLVGSDDAEAEMLLERRHAKGMTDGVWSGGVERF
                     IGFLDGLAAAGADWAVFVVAGPADRRDLLTDEVLPRLGSRIAAD"
     gene            531..1847
                     /locus_tag="VG709_01245"
     CDS             531..1847
                     /locus_tag="VG709_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HWC31736.1"
                     /translation="MRRVVIMGAAGRDFHNFNVVYRDDPETEVVAFTATQIPGIEGRR
                     YPSDLAGPRYPDGIPIELEEEVTRLVQAREVDEVVFAYSDVSHEHVMHRGSEVLAAGA
                     DFRLIGPRATMLEATKPVVAICAVRTGSGKSQTTRAVIRALRAMGRSPVVVRHPMPYG
                     DLVKQRVQRFASAEDLDEADATIEEREEYEAHIATGTVVYAGVDYEEILKAAQSEADV
                     VVWDGGNNDLPFFRPDVHLVVADPHRAGHETSYHPGEANFRMGDVIVINKVDTAEPEN
                     VDRVEETARRLNPDATIVRANSPVTVDEPDAVRGKRVLVVEDGPTLTHGEMTYGAGVV
                     AARAGGAAEIVDPREWVVGSLREVYVRYPVGPVLPAMGYSDEQLREMERTIEAVPADL
                     VVIATPVDLRHVASFSKPSVRVRYELDEAEGYPTIAEILAERLGNG"
     gene            1847..2785
                     /gene="arcC"
                     /locus_tag="VG709_01250"
     CDS             1847..2785
                     /gene="arcC"
                     /locus_tag="VG709_01250"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HWC31737.1"
                     /translation="MARVVVALGGNALLRRGDEDSAEVMRKNARLAAERIADIAASGW
                     EVVVTHGNGPQVGRILLQNEAARDWVHPMPLDVCGAESQGQIGYLLQVTIGDVFYERG
                     MERPVAALLTLTRVRPDDPAFENPTKPIGPHFDEGEGKRLAAERGWKMAATPEAQWRR
                     VVPSPHPYSIVEAPLIRQLAESGAIVIGGGGGGVPVIEEGPRLVGGEAGGDKDWAGAI
                     LARDVEAEVLLICTDVEGVYDRYGEPDQRLVERMSVEETKRMLDAGAFPPGSMGPKVE
                     SAVEFVSGGGKRAIITALDDAPDALEGNAGTTITPG"
     gene            2863..3534
                     /locus_tag="VG709_01255"
     CDS             2863..3534
                     /locus_tag="VG709_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWC31738.1"
                     /translation="MARIRVAAYNVQMLRAGTRRVAAALADVRPDVLILNETGYVGLK
                     LRRFARRLGMRRSSGLHGIRRIPNAVFVRAPWRIVGSRTLRFPRLHRTVRRGAVVSEV
                     ARAGARLWVVAVHLGLAADERRRHAEVLTDDLAGVRPVVIGGDLNEDPSGAAATWIRS
                     RYWDAFGDVLAGEAPTFPSHSARARIDYVFVSDAINVERAWVHVAAGDASDPLPVFAD
                     LEGAD"
     gene            complement(3535..4290)
                     /locus_tag="VG709_01260"
     CDS             complement(3535..4290)
                     /locus_tag="VG709_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC31739.1"
                     /translation="MSAQVMAGADAFAIDAGPSGVLLLHGFTGNPASMRVVGAWLAER
                     GHSVSAPRYPGHGTRWQDLADTTWEDWAGEAERALEDLAGRTDGLVVLGQSMGGAMAL
                     HLAANRADVVRGVALVNPYVLDRRIAPAFLLSPVLRSWKGVGGDIKKAQADELAYPRI
                     PMRAIAQLAAFLKVVRRELRQVRQPLILFRSVQDHVVPKGSAEFILERVGSTKKELVE
                     LPNSYHVACLDHDAELIAERTDAFARVLATATG"
BASE COUNT          622 a         1494 c         1639 g          604 t
ORIGIN      
        1 ctcgtgcccc cggcggcgac gaccgcgggc cctccggtct ggctgggcgc ccaggccgac
       61 gaggtggtcc ggatggcggc gcgagtcgcc gacggctgga acggctgggg catggacgtc
      121 gaggcgttcc gcgccaaggc cacgctcctc gatcgcgagg cgcgcgccgc cgggcgcacc
      181 gcggcggcga cgtgggccgg gatcgtgctg gtcggcagcg acgacgcgga ggccgagatg
      241 ctgctcgagc gccggcacgc caagggaatg accgacggcg tctggtcggg tggcgtcgag
      301 cggttcatcg gattcctcga cgggctcgcg gcagccggcg ccgattgggc ggtgttcgtc
      361 gtggccgggc cggccgaccg acgcgatctc ctcacggacg aggtgctgcc tcggctcggc
      421 tcgcggatcg ccgccgacta gcgggacccc cgagcatgcg acgccgaacc gtccccccgc
      481 tatcctgggg ttcccccgcg cccagggggc gcacccgacg aggaggactg atgagaaggg
      541 tcgtgatcat gggcgccgcc gggcgcgact tccacaactt caatgtcgtg tatcgcgacg
      601 accccgagac cgaggtggtc gccttcacgg cgacacagat ccccgggatc gagggccggc
      661 gatatccctc cgacctggct gggccccggt acccggacgg gataccgatc gagctcgagg
      721 aggaggtcac gaggctcgtc caggcccgcg aggtcgacga ggtcgtgttc gcgtactccg
      781 acgtctcgca cgagcatgtg atgcaccggg ggagcgaggt gctcgcagcg ggtgccgact
      841 tccgcctgat cggcccccgc gccacgatgc tcgaggcgac gaagccggtc gtcgcgatct
      901 gcgcggtccg gaccggctcc ggcaagagcc agacgacgcg cgcggtgatc cgcgcgctgc
      961 gcgccatggg acgctcgccc gtcgtcgtgc gacatccgat gccgtacggc gacctggtga
     1021 agcagcgcgt gcagcggttc gcctctgccg aggatctcga cgaggccgac gcgacgatcg
     1081 aggagcgcga ggagtacgag gcgcacatag cgaccggcac ggtcgtgtac gcgggcgtcg
     1141 actacgagga gatcctgaag gcggcgcagt cggaggccga cgtcgtcgtg tgggacggtg
     1201 gcaacaacga cctcccgttc ttccgcccgg acgtgcacct cgtcgtggcg gacccccatc
     1261 gcgccgggca cgagacgagc taccaccccg gcgaggcgaa cttccggatg ggggacgtga
     1321 tcgtcatcaa caaggtcgac acggccgagc cggagaacgt cgatcgcgtc gaggagaccg
     1381 ctcgtcgtct gaaccccgac gcgacgatcg tccgcgcgaa cagccccgtc acggtcgacg
     1441 agccggacgc cgtgcggggg aaacgcgtcc tggtagtgga agacggcccg acgctcaccc
     1501 acggcgagat gacgtacggc gcgggcgtcg tggcggcacg ggccggcggc gccgcggaga
     1561 tcgtcgatcc acgcgagtgg gtcgtcggct cgctccgcga ggtctacgta cggtacccgg
     1621 tcgggcctgt gctgccggcg atgggctact cggacgagca gctccgcgag atggagcgca
     1681 cgatcgaggc cgtccccgcc gatctggtcg tgatcgcgac gccggtcgac ctccgtcacg
     1741 tcgcgtcctt ctcgaagccg tcggtgcgcg tccgctacga gctggacgag gccgagggct
     1801 acccgacgat cgcggagatc ctcgccgagc gcctcgggaa cgggtgatgg cgcgggtcgt
     1861 ggtcgcgctc ggcggcaacg cgttgctgcg ccgaggcgac gaggactcgg ccgaggtcat
     1921 gcggaagaac gcgcggctcg ccgccgagcg catcgccgac atcgcggcca gcggctggga
     1981 ggtcgtcgtc acgcacggaa acggccccca ggtgggacgg atcttgctgc agaacgaggc
     2041 ggcacgggac tgggtccacc cgatgccgct cgacgtctgc ggcgccgaga gccaggggca
     2101 gatcggctac ctactccagg tcacgatcgg cgacgtgttc tacgagcgcg gcatggagcg
     2161 cccggtcgcg gcgcttttga cgctgacgcg cgtgcggccg gatgaccccg ccttcgagaa
     2221 cccgacgaag ccgatcggcc cccacttcga cgagggcgag gggaagcgcc tcgccgccga
     2281 gcgcgggtgg aagatggccg cgactcccga ggcgcagtgg cgtcgcgtcg tcccgtcgcc
     2341 gcacccgtac tcgatcgtcg aggcgccgct gatccgccag ctggcggaat ccggcgcgat
     2401 cgtgatcgga ggcggtggcg gcggcgtgcc ggtgatcgag gaggggcccc ggctcgtcgg
     2461 cggcgaggcc ggcggcgaca aggactgggc cggggcgatc ctcgcccgcg acgtcgaagc
     2521 agaggtgctg ctgatctgta ccgacgtcga gggcgtctac gaccggtacg gggagccgga
     2581 ccagcggctc gtcgagcgca tgtcggtcga ggaaacgaag cggatgctcg acgcgggcgc
     2641 gttccccccg ggctcgatgg ggcccaaggt cgagtcggcg gtcgagttcg tctcgggcgg
     2701 cggcaagcgc gcgatcatca ccgcgctcga cgacgcgccc gacgcgctcg agggcaacgc
     2761 cggaaccacg atcaccccgg gctaaccggt cggcggcgtt ccatcgaggc gaccctgcgc
     2821 gcctgttgtg acgcgcgggt gcgcggcgcg tacgcttcgc gcgtggctcg gatccgcgtc
     2881 gccgcgtaca acgtgcagat gcttcgggcg gggacgcggc gcgtcgcggc cgcgctcgcc
     2941 gacgtgcgcc ccgacgtgct gatcctgaac gagaccggct acgtcgggct caagctccgg
     3001 cggttcgccc gacggctcgg gatgcgccgg tcgagcggcc tccacggcat ccgccggatc
     3061 ccgaacgccg ttttcgtgcg ggctccatgg cggatcgtgg gctcgcggac gctgcggttt
     3121 ccccggctcc accgtacggt tcgccgcggg gcggttgtga gcgaggtcgc tcgcgccgga
     3181 gcgcgcctgt gggtcgttgc cgtgcacctg gggctcgccg ccgacgagcg acggcggcat
     3241 gcggaggtgc tgaccgacga cctcgccggc gtgcgccccg tcgtgatcgg cggcgatctc
     3301 aacgaggacc cctcgggtgc ggcggcgacg tggatcagga gtcggtactg ggacgcgttc
     3361 ggggacgtcc tggcgggcga agcacccacg ttcccgagcc actcggcgcg ggcgcgcatc
     3421 gactacgtgt tcgtgtccga cgcgatcaac gtcgagcgcg cgtgggtcca cgtggctgcc
     3481 ggcgacgcct cggatcccct tccggtcttc gcggacctcg agggcgccga ctgatcagcc
     3541 ggtcgccgta gcgaggacgc gggcgaacgc gtcggtgcgc tcggcgatga gctccgcgtc
     3601 gtggtcgagg caggcgacgt ggtacgagtt cgggagctcg acgagctcct tcttcgtcga
     3661 gccgacgcgt tcgaggatga actcggcgct gcccttcggg accacgtggt cctggacgga
     3721 cctgaagagg atcagcggct gccgcacctg ccgcagctcc cggcggacga ccttgaggaa
     3781 ggcggcgagc tgcgcgatcg cccgcatcgg gatgcgcggg taggcgagct cgtcggcctg
     3841 cgccttcttg atgtcccctc ccacgccctt ccacgacctc aagaccggcg atagcaggaa
     3901 cgcgggtgcg atccgccggt ccaggacgta tgggttgacg agggcgacgc cgcggacgac
     3961 gtcggcgcgg ttcgccgcca ggtggagcgc catggccccg cccatcgatt ggccgaggac
     4021 gacgagtccg tcggtcctgc cggcgaggtc ctccagcgcg cgctccgcct ctccggccca
     4081 gtcctcccac gtcgtgtcgg cgagatcctg ccacctggtc ccgtggcccg ggtacctcgg
     4141 tgccgacacc gagtgaccac gctcggcgag ccatgcgccg acgacgcgca tgctggccgg
     4201 attccccgtg aacccgtgca ggagcagcac gcccgacggc ccggcgtcga tggcgaaggc
     4261 gtcggcgccc gccatcacct gagcgctcat cggatcagtg accggagccg ggagatcacc
     4321 gcgtcgggat cgaagggctt ggcgaagaag ttgtcgcct
//
LOCUS       DASZOB010000080         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_153022, whole genome shotgun sequence.
ACCESSION   DASZOB010000080 DASZOB010000000
VERSION     DASZOB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153022"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VG709_01265"
     CDS             complement(<1..886)
                     /locus_tag="VG709_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWC31740.1"
                     /translation="MTLPLAQELNQPWWIHVIKAFVVANLVLGAFAYLTWVERKLLGR
                     MQLRYGPNRTGPFGLLQPIADLVKLLRKASFFPAAAVNVLYIAAPAVAGFTALASFSV
                     IPFGGVWQIGPYDVTGRVVDVPISLLLLFALGAVGIYAFLVGGWSSRSKYSLLGSMRT
                     SAQLVSYEVSLGLAVLGVVIMGESLSLVEIVERQSETIWFVVPQFVGFLAFFVAGLAE
                     TNRTPFDLPEAESELVAGYHTEYSGMRFGLFQTAEYINMITFSALAVTLFLGGWHGPV
                     LPGPLWFVLKLAVFFLMFM"
     gene            complement(1144..>1645)
                     /locus_tag="VG709_01270"
     CDS             complement(1144..>1645)
                     /locus_tag="VG709_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWC31741.1"
                     /translation="PRTPNGRAVATAWRSAGEGRGTEPAADGEIGALIVSGDEALYDP
                     RVPDLARRARFVLTTSMFMGDHTLWSHLVIPGTSYLERDGTVVNVEGRPQRLRRAVDP
                     PFPDELEFLARVGDELGLPIAPWPHDALPDEHAPLPPRADLGDTQQPEPTPATNGSGR
                     GLELLT"
BASE COUNT          271 a          547 c          616 g          211 t
ORIGIN      
        1 acatgaacat caagaagaag acggcgagct tcagcacgaa ccagagcggg ccggggagca
       61 cggggccgtg ccagccgccg aggaagagcg tgacggcgag ggccgagaac gtgatcatgt
      121 tgatgtactc ggcggtctgg aagaggccga agcgcatgcc gctgtactcg gtgtggtagc
      181 cggcgacgag ctcggactcg gcttccggaa ggtcgaaggg cgttcggttg gtctccgcga
      241 gcccggcgac gaagaaggcg aggaacccga cgaactgcgg gacgacgaac cagatcgtct
      301 cgctctgcct ctcgacgatc tcgacgagcg agagcgactc gcccatgatc acgacgccga
      361 gcacggcgag cccgagcgag acctcgtacg agacgagctg cgccgaagtc cgcatcgagc
      421 cgagcagcga gtacttcgac ctgctcgacc agccgccgac gaggaaggcg tagatcccga
      481 ccgctccgag tgcgaagagg aggaggaggg agatcgggac gtccacgacg cgcccggtga
      541 cgtcgtaggg gccgatctgc cacacccctc cgaatgggat gacgctgaac gacgcgagcg
      601 cggtgaaccc ggcgacggcc ggagccgcga tgtagaggac gttgaccgct gcggcgggga
      661 agaagctcgc cttgcggagc agcttcacga ggtcggcgat cggctggagg aggccgaaag
      721 gtccagtccg gttcgggccg tagcggagct gcatgcgccc gaggagcttc cgctccaccc
      781 acgtcagata cgcgaacgcg ccgaggacga ggttcgcgac gacgaacgcc ttgatcacgt
      841 ggatccacca cggctggttg agctcctggg cgagcggcag ggtcacgtcg ccttcgtcac
      901 ctccccgtgt cgctccagcc cgcccgcgtg ctcggaggcg acgcggacgc cgccaggtcg
      961 caggcggcgg ttgacgcgcg cgacgagttc gcgcgtcgtt ccgttcgact cgacggagat
     1021 cgcgtccccg gtgcggatgc cgcgctcgcc cgcgtccgca gccgagagct cggcctggag
     1081 gagggggcgc tggaaggcgc gcgcgtcgat ccgcgcgacg ccgggccccg cgaaggccga
     1141 ccgttaggtc aggagctcga gcccccggcc ggagccgttc gtcgccggcg tcggctcggg
     1201 ttgctgcgtg tcgccgaggt ccgcgcgcgg cggaagcggc gcgtgctcgt cggggagcgc
     1261 gtcgtgcggc cagggggcga tcggcaggcc gagctcgtcc cccacgcgcg ccaggaactc
     1321 gagctcgtcg gggaacggcg ggtcgacggc gcggcgcagc cgctgcggcc gcccctcgac
     1381 gttcacgaca gtgccgtcgc gctcgagata gctcgttccc ggaatgacga ggtgcgacca
     1441 caacgtgtgg tcgcccatga acatcgaggt cgtgagcacg aagcgcgccc gtcgcgcgag
     1501 gtcggggacg cgggggtcgt agagggcctc gtcaccagag acgatgagcg cgccgatctc
     1561 cccgtccgcc gccggctccg tcccacgtcc ttcgcccgcg ctccgccacg ccgtcgcgac
     1621 tgcgcggccg ttcggcgtcc ggggg
//
LOCUS       DASZOB010000081         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_163174, whole genome shotgun sequence.
ACCESSION   DASZOB010000081 DASZOB010000000
VERSION     DASZOB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163174"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VG709_01275"
     CDS             <1..748
                     /locus_tag="VG709_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWC31742.1"
                     /translation="WNAVWHLDVDVTNVTGALAAVNVAGPRARDVMARLTDLDVSNEA
                     FKYLDARRAHVAGVPSLVLRIGFVGELGYEIHFPSPYGESLWDAILAEGGEYGVRPFG
                     LEPQRILRLEKMHILVGQDTDSESNAFEASMPWIVKLDKDDFVGKWSLEQVRERGLRE
                     RLVGFEMADGIGAVPAEGGQIVVEGRPGGRVTSARWSPELERAIGLAWVPADLAAEGS
                     ELTIRMDGGVEKARVRLRPFFDPDGARLRS"
     gene            745..1308
                     /locus_tag="VG709_01280"
     CDS             745..1308
                     /locus_tag="VG709_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31743.1"
                     /translation="MTSLDFLSLDGVRADDRFRPLARSSMERRLREAGAAFAERDGWL
                     VATHVPGQEDRRLVVRDVTHLHRVRESADGVLVEFAPGDGREPVVGGHGWAGGGDRLP
                     EATPDVSAGYAALHLEGPGATTLLRRLTEMPLDALPAAGAVAHVRAWVVEDGEESYRL
                     LFAQEYGHYLWEVVVDAAEPLGGGPGA"
     gene            1305..2519
                     /locus_tag="VG709_01285"
     CDS             1305..2519
                     /locus_tag="VG709_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC31744.1"
                     /translation="MKDVFRVRRMWRPRGELRKRYDVVVVGGGSHGLATAYYLAKTHG
                     VKNVAVLEKSYIGSGAAGRNTTIVRSNYRTPEGAEFYKESVRLYEGLSVELDFNMLFS
                     QQGHLTLAHSDRAMITMTERAEVNQLLGIDSRLIYRDEIARLCPEMDLSEDVTWPVMG
                     ALYHPPGGIIRHDAVVWGYARGADRHGVEIHPYTEVTGITTSDGRVTGVQTTRGDVEA
                     DVVVSCVAGWSTLVCDLAGVPLPITPHILQALVTEPLKPLLDVVIVSSQMHVYISQTD
                     RGEFLMGAEIEPYTTYKGQSTFPFLEYSARHTLELFPQLETVKVLRQWTGLCDLSPDY
                     SPILGKTEVENFHVSAGWGTYGFKAAPIVGVTLAELVATGTTPRLIAPFELERFYRDE
                     LVSELAAAAGSH"
BASE COUNT          387 a          912 c          940 g          376 t
ORIGIN      
        1 gtggaacgcc gtctggcacc tcgacgtcga cgtcacgaac gtgaccggcg cgctcgcggc
       61 cgtgaacgtg gccggcccgc gcgcgcgcga cgtcatggcg cggctcacgg acctcgacgt
      121 ctcgaacgag gcgttcaagt atctggacgc gcggcgcgcc cacgtcgcag gcgtcccgtc
      181 gctcgtcctt cgcatcgggt tcgtcggcga gctcgggtac gagatccact tcccgagccc
      241 gtacggcgag tccctctggg acgcgatcct ggcggagggc ggcgagtacg gcgtccggcc
      301 gttcggcctc gagccgcaac ggatcctgcg gctcgagaag atgcacatcc tcgtcggcca
      361 ggacaccgac tccgagtcga acgcgttcga ggcgagcatg ccgtggatcg tgaagctcga
      421 caaggacgac ttcgtcggca agtggtcgct cgagcaggtg cgcgagcggg gcctgcgcga
      481 gcgcctcgtc ggcttcgaga tggcggacgg gatcggcgcc gtcccggccg aaggaggtca
      541 gatcgtcgtc gaagggcgtc ccggcggccg cgtcacgagc gcgcggtgga gccccgagct
      601 cgagcgcgcg atcggcctcg cctgggtacc ggcggacctc gccgccgagg ggtccgagct
      661 cacgatcagg atggacggcg gcgtcgagaa ggcgcgcgtc cggttgcggc cgttcttcga
      721 tcccgacggc gcgaggctgc gctcgtgacg agcctcgact ttctctcgct ggacggcgtc
      781 cgcgcagacg accgcttccg cccgctcgcg cgctcgtcca tggagcggcg cctccgcgag
      841 gcgggcgcgg cgttcgccga gcgagacggc tggctggtcg cgacgcacgt ccccggccag
      901 gaggatcgcc gcctcgtcgt cagggacgtc acgcacctcc accgtgtccg cgagagcgcc
      961 gacggcgtcc tcgtcgagtt cgccccgggc gacggccgcg agcccgtcgt cggcgggcac
     1021 gggtgggcgg gcggcggcga ccgcctgccg gaggcgacgc ccgacgtgag cgctggatac
     1081 gccgcgctgc acctcgaggg accgggcgcg acgacgctgc tccggcgcct gaccgagatg
     1141 ccgctcgacg cgctccccgc cgcgggggcc gtggcgcacg tgcgcgcgtg ggtcgtcgag
     1201 gacggcgagg agtcgtaccg tctcctcttc gcgcaggagt acggccacta cctctgggag
     1261 gtcgtcgtcg acgcagcgga gccgctcgga ggaggtccgg gcgcgtgaag gacgtcttcc
     1321 gcgtgcggcg gatgtggcgg ccgcgtggcg agctgcgcaa gcgctacgac gtcgtcgtcg
     1381 tcggcggcgg ctcgcacggc ctcgcgaccg cgtactacct cgcgaagacg cacggcgtga
     1441 agaacgtcgc agtcctcgag aagagctaca tcggctcggg cgcggcgggc aggaacacga
     1501 cgatcgtccg ctcgaactac cggacgcccg agggcgccga gttctacaag gagagcgtcc
     1561 gcctctacga gggcctttcc gtcgagctcg acttcaacat gctcttctcg cagcaggggc
     1621 acctgaccct cgcccactcc gaccgcgcga tgatcacgat gaccgagcgc gccgaggtga
     1681 accagctcct cgggatcgac agccgcctga tctatcggga cgagatcgcg cgcctctgcc
     1741 cggagatgga cctctccgag gacgtgacgt ggccggtcat gggggcgctc taccacccgc
     1801 ccggcgggat catccgtcac gacgccgtcg tctgggggta cgcgcgcggc gccgaccgcc
     1861 atggggtcga gatccacccg tacacggagg tgacggggat cacgacgtcg gacgggcgcg
     1921 tgacgggcgt gcagacgacc cggggagacg tcgaggccga cgtggtcgtg agctgcgtcg
     1981 ccggctggtc gacgctcgtc tgcgacctcg caggcgtccc gctcccgatc acgccgcaca
     2041 tcctgcaggc gctggtcacc gagccgctga agccgctgct cgacgtggtc atcgtctcgt
     2101 cgcagatgca cgtctacatc tcgcagaccg accgcggcga gttcctgatg ggcgcggaga
     2161 tcgagcccta cacgacgtac aagggccaga gcacgttccc gttcctcgag tactcggcgc
     2221 gccacacgct cgagctcttc ccgcagctcg agaccgtgaa ggtgctccgt cagtggacgg
     2281 gcctctgcga cctctcgccg gactacagcc cgatcctcgg gaagacggag gtcgagaact
     2341 tccacgtctc cgccggctgg ggaacctacg gcttcaaggc ggcgccgatc gtcggtgtca
     2401 cgctcgccga gctcgtcgcg acggggacga ctccgcgcct gatcgcgccg ttcgagctgg
     2461 aacgcttcta ccgcgacgag ctcgtctccg agctggccgc cgccgccggc agccactagc
     2521 cagaggagtg ctagatgaag agcagtctcg agatcgctca agaggcgcag ctgctgccga
     2581 tcgacgagat cgcggagcgg atcggactcg agact
//
LOCUS       DASZOB010000082         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_21918, whole genome shotgun sequence.
ACCESSION   DASZOB010000082 DASZOB010000000
VERSION     DASZOB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21918"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..578)
                     /gene="msrA"
                     /locus_tag="VG709_01290"
     CDS             complement(57..578)
                     /gene="msrA"
                     /locus_tag="VG709_01290"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWC31745.1"
                     /translation="MTTEQATFGAGCFWGVEYVYRRVPGVTDVKVGYSGGITPNPTYQ
                     EVCSHTTGHAEVTQVTFDPEKVTYEQLLEVFWAMHDPTQVDRQGPDIGDQYRSVIFTH
                     SDEQRTAAEASRERAQERFPRPIATEIQPLDVFYDAEDYHQAYYEKNGKTPYCHVVPV
                     ATLEQLGLVSAAR"
     gene            complement(606..1136)
                     /locus_tag="VG709_01295"
     CDS             complement(606..1136)
                     /locus_tag="VG709_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWC31746.1"
                     /translation="MISDVASFVRYFGGIHRRTVRAVSALPASAERWRPSSGDGEAAW
                     GVPEIVRHIAEARLFFASAYAGDGWVWDSWPEPVDAKPTWVPALERSAEQLAGLLEGT
                     QEAWLSRKVEAIGGDGVMLSGWRVLMMMVEHEVHHRAQISTYAGLNGWPVHQTFDRTN
                     EWVLERREEQVRGRRR"
     gene            1193..>1544
                     /locus_tag="VG709_01300"
     CDS             1193..>1544
                     /locus_tag="VG709_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31747.1"
                     /translation="MAAWTSLEGSLASAPAAVSWGDGEVEVFAIQDDGALWNRYWDLQ
                     SWHAWESLGGSFRGEPAASARDANRIDVLAIGTDGALRHRFWNGSQWVPWREIADAPR
                     DAKAGACSWRGARLD"
BASE COUNT          218 a          563 c          515 g          248 t
ORIGIN      
        1 gcatcacgcc gtccagcgtg aggaacctcg ctgacgatga cgcgtctcac gcgacgtcag
       61 cgagccgccg agacgagccc gagctgctcg agggtcgcga cggggacgac gtggcagtac
      121 ggcgtcttgc cgttcttctc gtagtacgcc tggtggtagt cctcggcgtc gtagaagacg
      181 tcgagcggct ggatctcggt cgcgatcggt cgcgggaacc gctcctgcgc gcgctcgcgt
      241 gaggcctccg cggcggtgcg ctgctcgtcc gagtgcgtga agatcacgct gcggtactgg
      301 tcgccgatgt ccgggccctg ccggtccacc tgcgtcgggt cgtgcatcgc ccagaacacc
      361 tcgagcagct gctcgtacgt gaccttctcc ggatcgaatg tcacctgcgt gacctcggcg
      421 tggcccgtcg tgtgcgagca cacctcctgg tacgtcgggt tcggcgtgat ccctccgctg
      481 tagccgacct tcacgtcggt cacgcccgga acgcggcggt acacgtactc cacgccccag
      541 aagcatcccg ctccgaacgt cgcctgctcg gtcgtcatgt ctccctcctc agtttgtcgt
      601 ctctctcaac gccggcgccc ccgcacctgt tcctcgcggc gctcgagcac ccactcgttc
      661 gtgcggtcga acgtctggtg gaccggccag ccgttcagcc cggcgtaggt cgagatctgc
      721 gcacggtgat ggacctcgtg ctcgaccatc atcatgagga cgcgccaccc cgacagcatc
      781 acgccgtcgc cgccgatcgc ctcgaccttc cgcgagagcc acgcctcctg cgtgccctcg
      841 agcagtccgg cgagctgctc cgccgaccgc tcgagcgccg gcacccacgt cggcttcgca
      901 tcgaccggct ccggccacga gtcccagacc cacccgtcgc cggcataggc gctcgcgaag
      961 aacagtctcg cctcggcgat gtggcgcacg atctccggga cgccccacgc ggcctcgccg
     1021 tccccggagg aaggccgcca gcgttcggca ctcgcgggaa gcgccgagac ggcccgcacg
     1081 gtcctgcggt ggatacctcc gaaatagcgg acgaacgacg cgacgtcgga gatcacggta
     1141 cctcgcagac caccctagac tgcttgcaac catcgcgctc ggggaggtcg ccatggcagc
     1201 gtggacgtcg ctcgaaggtt cgctcgcatc cgcccccgcg gccgtatcgt ggggcgacgg
     1261 agaggtcgag gtcttcgcga tccaggacga cggcgcgctc tggaaccggt actgggacct
     1321 tcagagctgg cacgcgtggg agtcgctcgg cgggtcgttc cgcggggagc cggctgcctc
     1381 ggcccgcgac gcgaaccgga tcgacgtcct cgcgatcggg accgacggcg ctctccggca
     1441 ccgcttctgg aacgggtcgc agtgggtgcc gtggcgcgag atcgcggatg cgccacgcga
     1501 cgcgaaggcg ggcgcgtgct cctggcgcgg cgcgcgcctc gacg
//
LOCUS       DASZOB010000083         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_21938, whole genome shotgun sequence.
ACCESSION   DASZOB010000083 DASZOB010000000
VERSION     DASZOB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21938"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..679
                     /gene="pth"
                     /locus_tag="VG709_01305"
     CDS             101..679
                     /gene="pth"
                     /locus_tag="VG709_01305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWC31748.1"
                     /translation="MAWLVVGLGNPGDRYASTRHNIGWWTLEDLAERLGARLRKVRFH
                     PLEVGETKSHGESVLLVRALTFMNEVGPPIASFARRRKVADDRIVVVHDDIDLGFGAL
                     RVKRGGSTAGHRGLNSLAGALRSPDFYRVRLGIGRPPGRKDPADYVLERFAKRERGDA
                     EALAEEGAEAVLTLVAEGLERAQDRHNRSGPP"
     gene            complement(686..1639)
                     /locus_tag="VG709_01310"
     CDS             complement(686..1639)
                     /locus_tag="VG709_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31749.1"
                     /translation="MRRLVVLLLLPACVTASGEATSISAAGAPTCTIFPRNSFWHADV
                     RSLPVHPRSADWIRSMGGPNARLHPDFGPSEGGRPYGIPYEVVGASDEKVGIEFLYEE
                     ESDPGPYPFGPDTPIEQGGDRHALMLHRNECFLYELFDADWNDGDPTAGSGAIFDLRS
                     NRLRPAGWTSADAAGLSGFAGLVRRDEVEAGVIDHAIRVTATPTRSRYVWPARHEASD
                     VTDRRVPPMGAWFRLRRGFDLSGFRRETRVVLRAMKVHGLVVADNGSNWFFGGASEEG
                     WSEPVLDELKSVRVRAFQAVDTRPMLVSRDSGRVRPRYVGG"
     gene            1685..1819
                     /locus_tag="VG709_01315"
     CDS             1685..1819
                     /locus_tag="VG709_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31750.1"
                     /translation="MYSKVKVRGQAVVAAEPDDVHLVLEVTALEEAPDRAFDVGPRGP
                     "
     gene            1848..>2121
                     /locus_tag="VG709_01320"
     CDS             1848..>2121
                     /locus_tag="VG709_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWC31751.1"
                     /translation="MRKGFRASSSVTVRIDDVAIIGKLMSEATGRARAEVRGPWWGVK
                     PDNAARAEACRLAATDARRKAEAYAAALGKRLGDVLEVVGTVRSSRV"
BASE COUNT          316 a          712 c          797 g          296 t
ORIGIN      
        1 gggcgccgag gaggccgcgg agggtgccgc cgggcctggc gaggcggaag tcccgcccgc
       61 cgagggcggc ggggagcagg gctagccctc gtcgctggag gtggcctggc tcgtcgtcgg
      121 gctgggcaat ccgggagacc ggtacgcgtc cacgcgccac aacatcgggt ggtggacgct
      181 cgaggacctc gccgaacggc tcggcgcgcg cctgcggaag gttcggttcc atccgctcga
      241 ggtcggggag acgaagtcgc acggcgaatc cgtgctgctc gtccgagcgc tcacgttcat
      301 gaacgaggtc ggccctccga tcgcgtcgtt cgcgaggcga cggaaggttg ccgacgatcg
      361 gatcgtggtg gttcacgacg acatcgatct cgggttcggc gcgctgcgag tcaagcgcgg
      421 cggcagcacc gctgggcatc gcgggctgaa ctctctcgcc ggtgcgcttc gttcgccgga
      481 cttctatcgc gtgcgcctcg ggatcgggcg cccgccggga cggaaggatc ccgccgacta
      541 cgtcctcgag cggttcgcga agcgcgagcg tggggacgcc gaggcgcttg cggaggaggg
      601 cgccgaggcc gtcctcacgc tggtcgccga ggggctcgag cgcgcgcagg accggcacaa
      661 ccggagcggc cctccgtagg tcggctcagc cacccacgta ccgggggcga acacggccgg
      721 agtcgcgcga aaccagcatc ggccgcgtat ccacggcctg gaaggcacgg acacggaccg
      781 acttcagctc gtcgagtacg ggctcggacc agccctcctc cgaggcgccc ccgaagaacc
      841 agttggagcc gttgtcggcg acgacgaggc cgtggacctt catcgcgcga aggacgacac
      901 gcgtctccct gcggaagccg ctgaggtcga atcctcgacg gagccggaac caggcgccca
      961 tcggcggcac acgacggtcg gtcacgtcgc tcgcctcgtg gcgggccggc cagacgtacc
     1021 ggctgcgggt cggtgtcgcg gtcacgcgta tggcgtgatc gatcacgccg gcctcgacct
     1081 cgtcgcgccg aacgaggccc gcgaaccccg agagccccgc ggcgtccgcc gacgtccacc
     1141 ccgccggacg gaggcggttc gatcgcaggt cgaagatcgc gccggagcct gccgtcgggt
     1201 cgccgtcgtt ccagtcggcg tcgaacagct cgtagaggaa gcactcgttc cggtgcagca
     1261 tcagcgcgtg ccggtcgcct ccctgctcga tcggcgtgtc gggcccgaac gggtatggcc
     1321 ccgggtcgct ctcctcctcg tagaggaact cgatgccgac cttctcgtct gaggcgccga
     1381 cgacctcgta ggggatcccg tacggccgtc cgccttcgga cggaccgaag tccggatgca
     1441 gccgcgcgtt cggtccgccc atggagcgga tccagtccgc gctccgcgga tggaccggaa
     1501 gcgagcggac atcggcatgc cagaagctgt tcctcgggaa gatcgtgcac gtgggcgcgc
     1561 ccgcggccga gatcgacgtc gcttcaccgc tggccgtgac gcaggccggc agcaggagca
     1621 agacgacgag tcggcgcatg cgtggattat cgtcgtcgcc gggtcgacgg ttgaggggag
     1681 cgcgatgtac tcgaaggtga aggtgcgcgg gcaggcagtc gtggcggcgg agccggacga
     1741 cgttcacctc gtcctcgagg tgacggcact ggaggaagcc cccgatcgcg cgttcgacgt
     1801 cgggcctcgt ggtccgtgac gaacgcgatt acgaacgcca gcgctgggtg cggaagggat
     1861 tccgggcctc gagctcggtc accgtacgga tcgacgacgt cgcgatcatc ggcaagttga
     1921 tgtccgaggc gacggggcgc gctcgcgcag aggtgcgagg gccgtggtgg ggcgtgaagc
     1981 ccgacaacgc ggcccgcgcg gaggcctgcc ggctcgcggc gaccgacgcg cggcgcaagg
     2041 ccgaggcgta cgccgccgcc ctcgggaagc ggctcggcga cgtccttgag gtcgttggaa
     2101 ccgtccgctc ctcccgggta c
//
LOCUS       DASZOB010000084         5664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_51999, whole genome shotgun sequence.
ACCESSION   DASZOB010000084 DASZOB010000000
VERSION     DASZOB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51999"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VG709_01325"
     CDS             <1..190
                     /locus_tag="VG709_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31752.1"
                     /translation="AVAAQPSRHLPGLHLAEQHHTRLLAGAESLDAAGDEREPVGVET
                     LGAGEQARVMLFREMEAG"
     gene            191..1264
                     /locus_tag="VG709_01330"
     CDS             191..1264
                     /locus_tag="VG709_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWC31753.1"
                     /translation="MAGGLRRYSMRHAILVATSNLSRGLFVGSMLAGAVLVVSAVLFY
                     FAERIRNEDDVAHIGDGFLWVTRTLLQQEPPFEPETFVGMVLYYVVVIAGVGIIAMAT
                     GAIATKLIQWVTRKDAGMADARYRDHIVICGWSPEGEQILAELHNDETIDKKPVVIAA
                     HLEANPTDDDLTTFVRGSPSDAETLRRAGIQYAETAIILADSSDPNATADQRDAMTLL
                     TALAVESANPNIHTCVEVIRSQNRPHFARAKVDEMIVSAELSGQLLATAAMNHGVTHV
                     LSDLMTRGAGEEIYSVDAPRSLVGKTFSAAIEELKRAADALPIAIGNKDEYEINPPAD
                     RTIRDGERLLVIAPRDPAEVVGA"
     gene            complement(1471..3108)
                     /locus_tag="VG709_01335"
     CDS             complement(1471..3108)
                     /locus_tag="VG709_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC31754.1"
                     /translation="MTRRNPIERVRSARRLASAGVLRRAVDAGFLDPRSMRAGLRASP
                     WLLGRGGSLGWLSQVNALARPGKPAVIDRGGTLTWREVDARANRLARGLGGLGVAPGD
                     TVSLLLRNGREFAEAVYACHKRGNVVAPLNTWAKAQELRGVLEQVRPKALLYDGRHAA
                     ELRDAVPEGTRLVEIGDGGELPGVEPYEALLARQEDGHLSPVTLRRGSRRLVIQTSGT
                     TGRPKGAARAAGAGELQALLARLSVVPYRREDVVLIPAPLFHSFGMLTLSIASLLGAT
                     MVLPESFEPEQTLQLIDLHRATAASLVPVMIRRTVSLPDEVRSRYDVSSLRILLASGS
                     ALTEPVRAAAREVFGDVLYDLYGSTETGWVAIATPGDMRARPGTVGRPVPGVDVAVFS
                     RDGKRLGHGEVGALYIRSNFLFEGYTSGERKDERRGYVSIGDVGHVDDRGYVFVEGRG
                     DDMVVVGGENVYPAEVEQAIERVAGVGEVAVVGIPDEEYGHVLAAYVVGTASEDLIRE
                     TCRRDLASFKVPRRIEVLDELPRTATGKVLKRELVRR"
     gene            complement(3105..4064)
                     /locus_tag="VG709_01340"
     CDS             complement(3105..4064)
                     /locus_tag="VG709_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HWC31755.1"
                     /translation="MDGVPHPPAQAFLGEPRHSGADVAERSGLDLETARRIRRALGMP
                     DAADDEVLYDDDDVEAMAALRSILDLGFPLHDVLTVTRAYGTALARVADAETRLFRER
                     VLEPMVADGATPDEVTERVRPVVDVLLRHLGRLLDQGHRRHLMVALDALGQRGGSESE
                     RAAAGFVDLVDFSRISGDLPGDELGAMVDSFEELAVEVCSNTGVRLVKVIGDAAMFVS
                     PEGRRALEAARTIVRLVEQNSVLPQARSGVDLGEVVAVGGDYFGRPVNTAARITAFAY
                     PGTVVVSRELLDDVGGDGVTTSRIGTERLKGVGRVELFKVKDA"
     gene            complement(4089..5165)
                     /locus_tag="VG709_01345"
     CDS             complement(4089..5165)
                     /locus_tag="VG709_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC31756.1"
                     /translation="MSVARPLLATVLVVALALPAGAADEDTIRCTDPSDDVDRLEIVV
                     EGEVATGRFALPAHEPTGLVVIGHGYGHTSRSWKHHMQTMAKHGLVAVTMDYRGTRIG
                     RKPEVGGSPTVRGWPAMDGAEDLIAAARLFDEWCAPPVVTLLGVSMGGNITGVALAKA
                     GERGIRGHDGSPLFDYWIDVEGVVNLVETYLEGRTACAALGVGCREVEDMHREAGGPI
                     EQRPEAYVERSVVARIGDVRASGVRGVIVSHGVDDGLVPYNQSREISTELRLVGVPTE
                     MRTVGLEDEDTERETTLTGHLVGQIEPGYDSPLAGHASERSTTHIVMEDALDRLWTLM
                     GGSQPANRECAVNGQVPARVLCTP"
     gene            complement(5235..>5664)
                     /locus_tag="VG709_01350"
     CDS             complement(5235..>5664)
                     /locus_tag="VG709_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC31757.1"
                     /translation="LRPEMATVPLPLRHRLVVRGIKRMFADPSRLPDPWYDAAADEFL
                     RVFKTPRGRIAFFSAARQIYLEQPYGDTGFWDRLPRLSRPALFVWGEKDRLVPWRFAP
                     YMERALPEAGSVVLPDCGHVPQYELPEETHWVIRGFLGAG"
BASE COUNT          860 a         2094 c         1927 g          783 t
ORIGIN      
        1 tgctgtagcg gcgcagccct cccgccatct acccggcctc catctcgcgg aacagcatca
       61 cacgcgcctg ctcgccggcg ccgagagtct cgacgccgcc ggggacgaac gcgagcccgt
      121 cggcgtcgag actctcggcg ccggcgagca ggcgcgtgtg atgctgttcc gcgagatgga
      181 ggccgggtag atggcgggag ggctgcgccg ctacagcatg cgccacgcga tcctcgtcgc
      241 gaccagcaac ctgagcaggg ggctgttcgt cggctccatg ctcgccggcg ccgtgctggt
      301 cgtctcggcg gtcctcttct acttcgcaga acgcatccgg aacgaagacg atgtcgcgca
      361 catcggcgac gggttcctgt gggtgacccg gacgctgctg cagcaggagc ctccgttcga
      421 gcccgagacg ttcgtcggca tggtcctcta ctacgtcgtg gtgatcgcgg gcgtcgggat
      481 catcgcgatg gcgaccggcg cgatcgcgac gaagctgatc cagtgggtga cgagaaagga
      541 tgcaggcatg gcggacgcgc gctaccgcga ccacatcgtg atctgcgggt ggagccccga
      601 gggcgagcag atcctcgcag agctgcacaa cgacgagacg atcgacaaga aacccgtcgt
      661 gatcgccgcg cacctcgaag cgaaccccac ggacgacgac ctcacgacgt tcgtacgcgg
      721 cagtcccagc gacgcggaga cgctgcggcg cgccggcatc cagtacgccg agaccgcgat
      781 catcctcgcc gacagctcgg acccgaacgc gacggccgac cagcgcgacg cgatgacgct
      841 gctcaccgcg ctcgccgtcg agagcgcgaa cccgaatatc catacgtgcg tcgaggtgat
      901 ccgctcgcag aaccgtccgc atttcgcgcg agcgaaggtc gacgagatga tcgtgagcgc
      961 ggagctctcc ggacagctgc tggcgaccgc cgcgatgaac cacggggtca cgcacgtcct
     1021 ctccgatctc atgacgcgtg gcgccggcga ggagatctac agcgtcgacg cgccgcgatc
     1081 gctcgtcggc aagacgttct ccgccgcgat cgaggagctc aagcgcgcgg ccgacgccct
     1141 gccgatcgcg atcggcaaca aggacgagta cgagatcaac ccgccggcag accgcacgat
     1201 ccgggacggc gaacggctcc tcgtgattgc gccgcgcgac cccgccgagg tcgtcggcgc
     1261 ctgacgtgcg ctccgtcgcg tcgcggctgg cgtacctgta cgtcggatcc tcggatgtcg
     1321 agcgcgacgt cggcttctac cgcgacgtcc tcggcgccga ggtcgtgtgg cgggtcgagg
     1381 gcatgggcgc ggaggcgccg cggtccggct cggcgacggt ccgatcgtgc ttatcgccga
     1441 ccatcgcgac gccccgagca cgtcggtgcc ctagcggcgt accagctcgc gctttaggac
     1501 cttgccggtc gccgtccgcg gcagctcgtc gaggacttcg atgcggcgcg gcaccttgaa
     1561 cgaggcgaga tcccggcggc acgtctcgcg gatcaggtcc tcgctcgcgg tgccgacgac
     1621 gtaggccgcg aggacgtggc cgtactcctc gtcggggatg cccacgacgg cgacctcgcc
     1681 cacgccggcg acgcgctcga tcgcctgctc gacctcggcg gggtacacgt tctcgccgcc
     1741 gaccacgacc atgtcgtcgc cgcgcccctc gacgaacacg tagccgcgat cgtcgacatg
     1801 gccgacgtcg ccgatcgaga cgtagccgcg gcgctcgtcc ttccgctcgc cggacgtgta
     1861 gccctcgaac aggaagttgc tccggatgta gagcgcgccg acctcgccgt gaccgagccg
     1921 cttcccgtcg cgagagaaga ccgcgacgtc gacgccgggg accggacggc cgaccgtgcc
     1981 tgggcgcgcc cgcatgtccc ccggcgtggc gatcgccacc catcccgtct cggtcgagcc
     2041 gtagaggtcg tagagaacgt cgccgaacac ctcgcgcgcc gcggcacgga ccggctcggt
     2101 cagcgccgaa cccgacgcca ggaggatccg cagcgacgac acgtcgtacc ggctccgcac
     2161 ctcgtccggc agcgagaccg tgcggcggat catgaccggg acgagcgagg cggccgtcgc
     2221 tcggtggaga tcgatcagct gcagggtctg ctccggctcg aacgattccg ggaggaccat
     2281 cgtcgcgccg aggagagacg cgatcgacag cgtcagcatc ccgaacgagt ggaagagcgg
     2341 cgcggggatc agcacgacgt cctcgcggcg gtacgggacg acggacagcc tcgcgagcag
     2401 cgcctgcagc tcgccggcgc cggcggcgcg ggcggccccc ttgggccgcc cggtcgtgcc
     2461 gctcgtctgg atcacgagcc tccgcgaccc gcggcggagc gtcacgggcg aaaggtggcc
     2521 atcctcctgc cgggcgagga gcgcctcgta cggctccacc ccggggagct caccgccgtc
     2581 cccgatctcg acgaggcgcg tgccctcggg gaccgcgtcg cggagctcgg ccgcgtgccg
     2641 cccgtcgtag agcagcgcct tcgggcgcac ctgctccagg acgccgcgga gctcctgcgc
     2701 cttcgcccac gtgttgagcg gcgcgacgac gttcccgcgc ttgtggcacg cgtacaccgc
     2761 ctcggcgaac tcgcgcccgt tccgcagcag cagcgagacc gtgtcgccgg gcgccacgcc
     2821 gagtccgccg agcccacggg ccaggcggtt cgccctggcg tccacctcgc gccacgtgag
     2881 cgtcccgccg cgatcgatga cggccggctt gcccggccgc gcgagagcgt tcacctgcga
     2941 gagccatccg agcgacccgc cgcgtccgag gagccacggc gacgcgcgca gtcccgcccg
     3001 catcgaccgg ggatcgagga accccgcgtc gaccgcgcgc cgcaacactc cggcgctcgc
     3061 gagccggcgc gcgctccgca cgcgctcgat cgggttgcgt ctcgtcatgc gtccttcacc
     3121 ttgaagagct cgacgcgtcc gacacccttc agccgctcgg tcccgatcct cgacgtggtg
     3181 accccatcgc ccccgacgtc gtcgagcagc tcccgcgaca cgacgacggt ccccgggtag
     3241 gcgaacgccg tgatccgcgc ggccgtgttc acagggcgcc cgaagtagtc accgccgacc
     3301 gctacgacct cgcccaggtc aacacccgat cgcgcctgcg ggaggaccga gttttgctcg
     3361 acgagccgga cgatggtccg agccgcctcg agggctcggc gaccctccgg cgagacgaac
     3421 atcgccgcgt ccccgatcac cttcacgagc cgcacgccgg tgttcgagca cacttccacg
     3481 gcgagctcct cgaagctgtc gaccatcgcg ccgagctcgt ctccgggaag gtcgccggat
     3541 atccgcgaga agtcgacgag gtcgacgaat ccggccgctg ctcgttcgga ctcgctcccg
     3601 cctcgctgcc cgagagcgtc gagagccacc atcagatggc ggcggtgccc ctggtcgagc
     3661 aggcgcccca ggtggcgcaa cagcacgtcg acgacgggac ggaccctctc cgtaacctcg
     3721 tcgggcgtcg cgccatccgc gaccatgggc tcgagcaccc gctccctgaa gagacgcgtc
     3781 tcggcatccg ccacacgggc cagcgcggtg ccgtacgcgc gcgtcacggt gagcacgtcg
     3841 tggagcggga acccaagatc gagaatcgag cgcagcgcag ccatcgcctc gacgtcgtca
     3901 tcgtcgtaga gcacctcgtc gtcggcggcg tcgggcatgc cgagcgcgcg ccggatgcgg
     3961 cgcgcggttt cgaggtcgag tccggagcgc tcggcgacgt cggcgcccga gtgcctgggt
     4021 tcaccgagaa aggcctgcgc gggagggtgc gggacgccgt ccaccggagc aagcctagcg
     4081 ttgcggcgct acggcgtgca gagcacgcgc gccgggacct gcccgttcac cgcgcactcg
     4141 cgattggcgg gctgcgagcc gcccatgagc gtccacagcc ggtcgagcgc gtcctccatc
     4201 acgatgtgcg tggtgctgcg ctccgatgcg tgcccggcaa gcggcgagtc gtagccgggt
     4261 tcgatctggc cgacgaggtg gcccgtgagg gtcgtctcgc gctcggtgtc ctcgtcctcc
     4321 agcccgaccg tccgcatctc ggtcggaacc cccacgaggc gaagctccgt ggagatctcg
     4381 cgcgactggt tgtacggcac gagcccgtcg tcgacgccgt ggctaacgat caccccgcgt
     4441 acccccgaag ccctcacgtc gccgatgcgc gcgacgacgg atcgctcgac gtacgcctcg
     4501 ggtcgctgct cgatcgggcc gccggcctcg cgatgcatgt cctcgacctc tcggcaccca
     4561 acgccgaggg cggcgcaagc ggtccgcccc tcgaggtacg tctccaccag gttgacgacc
     4621 ccctcgacgt cgatccagta gtcgaagagc ggcgaaccgt cgtggccgcg gatgccacgc
     4681 tcgcccgctt tcgccagcgc gacccccgtt atgttcccgc ccatgctgac gccgaggagc
     4741 gtcacgaccg gaggcgcaca ccactcgtcg aacagccgag ccgcggcgat caggtcctcg
     4801 gcgccgtcca tcgcgggcca ccctcgaacc gtcggcgatc cccccacctc tggtttccga
     4861 ccgatccgcg tcccgcggta gtccatcgtc acggcgacga gcccgtgctt cgccatcgtc
     4921 tgcatgtggt gcttccacga tctcgacgtg tggccgtacc cgtggccgat gacaacgagg
     4981 ccggtcggtt catgcgcggg gagcgcgaag cgaccggtcg cgacctcacc ttcgacgacg
     5041 atctcgagcc ggtcgacgtc atcgcttgga tccgtgcacc ggatcgtgtc ctcgtcggcg
     5101 gccccggcgg gcagcgcgag cgcgacgacg agcacggtcg cgagcagcgg gcgggcgacg
     5161 ctcatcccta tgtcgttctc cgcccgccgt ctccgtccct gccgcgctcg cctcggagac
     5221 ggcggactcc gtcgtcaccc cgcacccagg aacccccgga tcacccagtg cgtctcctcc
     5281 ggcagctcgt actgcgggac gtggccgcag tccggcagga cgaccgagcc cgcctcgggg
     5341 agcgcgcgct ccatgtaggg cgcgaaacgc catgggacca ggcgatcctt ctctccccac
     5401 acgaacagcg ccgggcgcga gagacgcgga agacgatccc agaaccccgt gtcgccgtac
     5461 ggctgctcga ggtagatctg gcgcgccgcc gagaagaacg cgatccggcc gcggggcgtc
     5521 ttgaagacac ggaggaactc gtcggcggcg gcgtcgtacc acgggtcggg gagccggctt
     5581 gggtccgcga acatgcgctt gatcccgcgg accaccaggc ggtggcgaag cggcagcggc
     5641 accgtcgcca tctcgggtcg cagc
//
LOCUS       DASZOB010000085         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_62483, whole genome shotgun sequence.
ACCESSION   DASZOB010000085 DASZOB010000000
VERSION     DASZOB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62483"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VG709_01355"
     CDS             complement(<1..1088)
                     /locus_tag="VG709_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWC31758.1"
                     /translation="MSRVNPRHSSSRGGDVLGDTALRAVLRVSGIALASALCALALLP
                     AVAAAGRAIDEFTRDFTRIGSDVDLSFPRIPERSTIYAADGSVLATLYLDENREYVRL
                     PKINRVARKAVLAIEDARFYEHEGLDLMGVARALAANVTAGEIEQGASTITQQLARNV
                     FPRSVGTERTLTRKIEEARVAMRLEQEYSKEQILELYLNEVYFGRGIYGIGTAAEYYF
                     GTTAKRLSLRQAATLAGLIAAPETYSPINDRDAAIARRNVVLSRMRELGMIAERRAER
                     AMARPLGLDITDVGNRRARFPYFVEYIKRQILHDPRLGDTFGETYRQRRRALFQGGLQ
                     IFTTLDPDLEDAGERIVRRHLPDPNDPEA"
     gene            1185..>1582
                     /locus_tag="VG709_01360"
     CDS             1185..>1582
                     /locus_tag="VG709_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWC31759.1"
                     /translation="MRRLALGALAAALAVLLAPHALGQEQRVVEVVELEGVIDPTTSE
                     YLLEQIDDAAEAGSEAVIVQLDTPGGLAVAMEEMVQAILDSDVPIVVWVGPPGAQAAS
                     AGVFLVYASDVAAMAGATSIGAAHPVDLSG"
BASE COUNT          221 a          545 c          573 g          243 t
ORIGIN      
        1 gcctcgggat cgttggggtc gggaaggtgc cggcgcacga tgcgctcgcc cgcgtcctcg
       61 aggtcagggt cgagcgtcgt gaagatctgg agcccgccct ggaacagcgc tcgcctccgc
      121 tgccggtagg tctcgccgaa cgtgtcgccc aaacgcgggt cgtggaggat ctgccgcttg
      181 atgtactcga cgaagtacgg gaagcgggcg cggcggttcc cgacgtcggt gatgtcgagg
      241 cccagcggtc tcgccatcgc gcgctccgct cgccgttcgg cgatcatccc gagctcgcgc
      301 atccgagaca gcacgacgtt ccgccgcgcg atcgccgcgt cgcggtcgtt gatcggggag
      361 tacgtctcgg gggccgcgat gagccccgcc agcgtcgccg cctgccgcag cgacaggcgc
      421 ttcgcggtgg tgccgaagta atactcggcg gccgttccga tcccgtagat cccgcgtccg
      481 aagtacacct cgttgaggta cagctcgagg atctgctcct tcgagtactc ctgctcgagg
      541 cgcatggcga cgcgtgcctc ctcgatcttg cgggtgagcg tgcgctccgt gccgacgctc
      601 cgcgggaaca cgttccgcgc gagctgctgc gtgatcgtcg aggcgccctg ctcgatctcg
      661 cccgcggtga cgttcgcggc gagcgcgcgc gcgacgccca tgaggtcgag accctcgtgc
      721 tcgtagaacc gtgcgtcctc gatcgcgagg acggccttcc gagcgacgcg gttgatcttc
      781 ggcagccgga cgtactcgcg gttctcgtcg aggtacagcg tcgcgaggac ggacccgtcg
      841 gcggcataga tcgtcgagcg ctcggggatc cgcgggaacg acaggtcgac gtcggatccg
      901 atcctcgtga agtcgcgcgt gaactcgtcg atcgcccggc ccgcggccgc gacggccggg
      961 agcagcgcga gcgcgcacag cgccgatgcg agcgcgatcc ccgacacgcg gagcacggcg
     1021 cgcagcgcgg tgtctccgag gacgtcgccg ccgcgtgagg aggaatggcg tgggttgacg
     1081 cgggacatgg gcgagacgcc tcaggatagc cgccgccgca gcgccgaacc agggttttcg
     1141 cggcggatac gcatccggag gccgccacct agaatcggga ccctgtgaga cgactcgcgc
     1201 tgggcgccct cgcagcggcg ctcgcggtcc tcctcgcacc gcacgcgctg ggacaggaac
     1261 agcgcgtggt cgaggtcgtc gagctcgagg gcgtgatcga tccgacgacg tccgagtacc
     1321 tcctcgagca gatcgacgac gccgcggagg ccggctcgga ggccgtgatc gttcagctcg
     1381 acacgccggg cgggctcgcg gtcgcgatgg aggagatggt ccaggcgatc ctcgattcgg
     1441 acgtccccat cgtcgtctgg gtcggaccgc ccggcgcgca ggccgcctcc gccggcgtgt
     1501 tcctcgtcta cgcctccgac gtcgccgcga tggccggggc gacgagcatc ggcgccgcgc
     1561 atccggtcga cctctccggc ga
//
LOCUS       DASZOB010000086         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_52111, whole genome shotgun sequence.
ACCESSION   DASZOB010000086 DASZOB010000000
VERSION     DASZOB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52111"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VG709_01365"
     CDS             complement(<1..429)
                     /locus_tag="VG709_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWC31760.1"
                     /translation="MRFKFSLAALVKVSVFTVIAVILTVLLGMKISNTGLFADTYALE
                     AEFTAAKGVFPGDAVKLAGVDVGRVKRTRIENGRAVVEFNVDREIPLPVDSTVGIRWR
                     NVIGLRFLYVYPGDSEKILEDGDRIPVEQTEDAGDIGEFLN"
     gene            complement(426..1463)
                     /locus_tag="VG709_01370"
     CDS             complement(426..1463)
                     /locus_tag="VG709_01370"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWC31761.1"
                     /translation="MALLRIVRTPLLVAVVVAGMFFGIKARYGDYAEYYYVKVGAPRA
                     GQLMRIGADVRESGVLIGTVSNIELHDRRVEFTLQIDPQYQIPASAEAFVTLKTLLGD
                     KFVDIRFDEWSEPFLADGATIKGHVGAELEDALQSGVQVFSAVDANDLATIVHELATG
                     ARDHGDEIARGLEAGAALSTVFRQTLRPQLQALRDFEVIFGELRQAADELNELADAVN
                     EGVPVYASPEAQEKLRRALIAVSYLSQHLGDLLIVERRDIGRMMDAGDRVLQTIAERT
                     EGLHNLVHGLYRYVFKLGGPPPVLRDNTAAAPFANFFGGDDFEETLDQVCGALPPEFP
                     EIPACRGRRTG"
     gene            complement(1463..>2221)
                     /locus_tag="VG709_01375"
     CDS             complement(1463..>2221)
                     /locus_tag="VG709_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC31762.1"
                     /translation="TFYWQAVKSLPKALHYRRQIGGTVSDITIGVGALVVGAGTFFVI
                     FSMSFFTGTQVGLQGFQGLRSIGAEAFTGLVSSWANTREITPLVAGVALAAQIGTSFT
                     AEIGAARISEEIDALETMAVPSMTYVVATRIWAAMITIVPLYLAALFASYIATEAIVT
                     KFYSLSSGTYTHYFRLFLPPIDVLYSLGKALIFAVIVTLIHCYYGYTASGGPAGVGRA
                     SGQAIRLSITTVVVVNLFLSILMWGGNETVKIAG"
BASE COUNT          371 a          747 c          757 g          346 t
ORIGIN      
        1 gttcaggaac tctccgatgt cgccggcgtc ctcggtctgc tcgacgggga tgcggtcgcc
       61 gtcctcgagg atcttctcgg agtcgcccgg atagacgtac aggaaccgca gtccgatcac
      121 gttgcgccag cggatgccga ccgtgctgtc gacgggaagc gggatctccc gatccacgtt
      181 gaactcgacg acggctcgtc cgttctcgat gcgggttcgc ttcaccctgc cgacgtcgac
      241 gccggcgagc ttgaccgcgt cgccggggaa gacgcccttc gcggcggtga actcggcctc
      301 gagcgcatac gtgtcggcga agaggccggt gttcgagatc ttcattccga ggagcaccgt
      361 gaggatcacc gcgatgaccg tgaagacgga gaccttcacg agcgccgcga gcgagaactt
      421 gaacctcatc cggtgcgcct cccgcggcat gccgggatct ccgggaactc gggcggcagt
      481 gcgccgcata cctggtcgag cgtctcctcg aagtcgtcgc cgccgaagaa gttcgcgaac
      541 ggcgccgctg ccgtgttgtc gcggaggacc ggcggcgggc cgcccagctt gaacacgtag
      601 cggtagagcc cgtggacgag gttgtggagc ccctcggtcc gctccgcgat ggtctgaagg
      661 acacggtcgc ccgcgtccat catgcggccg atgtccctgc gctcgacgat cagcaggtcg
      721 ccgaggtgct gcgacaggta cgacaccgcg atcagcgccc ggcgcagctt ctcttgggcc
      781 tccggcgagg cgtagaccgg cacgccctca ttcacggcgt ctgcgagctc gttgagctcg
      841 tcggccgcct gccgcagctc gccgaagatc acctcgaagt cgcgcagcgc ctgcagctgc
      901 gggcggagcg tctggcggaa caccgtcgag agcgcggcgc cggcctcgag cccccgcgct
      961 atctcgtcgc cgtggtccct cgcgccggtc gcgagctcgt ggacgatcgt cgcgaggtcg
     1021 ttcgcgtcca ccgccgagaa gacctggacg ccgctctgga gggcgtcctc gagctcggcc
     1081 ccgacgtgcc ctttgatcgt ggcgccgtcc gccaggaacg gctccgacca ctcgtcgaac
     1141 cggatgtcga cgaacttgtc gccgagcagc gtcttcagcg tcacgaaggc ctcggccgac
     1201 gccgggatct ggtattgggg gtcgatctga agcgtgaact ccacgcgacg gtcgtgcagc
     1261 tcgatgttcg agaccgtccc gatgagcacg ccgctctcgc ggacgtccgc gccgatccgc
     1321 atgagctggc cggcgcgcgg cgcgccgacc ttgacgtagt agtactcggc gtagtcgccg
     1381 tagcgcgcct tgatcccgaa gaacatgccc gcgacgacga cggcgacgag cagcggcgtc
     1441 cggacgatcc gcaggagcgc catcagccgg cgatcttgac cgtctcgttt cccccccaca
     1501 tcaggatgga gaggaagagg ttcacgacga cgacggtcgt gatcgagagc ctgatcgcct
     1561 ggcccgacgc gcgacctacg ccggcaggcc cgcccgacgc cgtgtagccg tagtagcagt
     1621 ggatcagcgt gacgatcacg gcgaagatca gcgccttccc gaggctgtac agcacatcga
     1681 tcggcggcag gaacaaccgg aagtagtggg tgtaggtgcc cgacgacagc gagtagaact
     1741 tggtgacgat cgcctcggtc gcgatgtacg aggcgaacag cgccgcgagg tacagcggca
     1801 cgatcgtgat catcgcggcc cagatgcgcg tggcgacgac atacgtcatc gagggcaccg
     1861 ccatcgtctc gagcgcgtcg atctcctccg agatgcgcgc ggcgccgatc tccgcggtga
     1921 acgacgtccc gatctgtgcg gcgagcgcga cgcccgcgac gagcggcgtg atctcgcggg
     1981 tattcgccca cgacgagacg agcccggtga acgcctcggc gccgatcgag cgcagcccct
     2041 ggaacccctg gagcccgacc tgcgtgccgg tgaagaacga catcgagaag atgacgaaga
     2101 aggtgccggc tccgacgacc agggccccga cgccgatcgt gatgtcggag accgtgccgc
     2161 cgatctgccg gcggtagtgc agcgctttgg ggagcgactt caccgcttgc cagtagaacg
     2221 t
//
LOCUS       DASZOB010000087         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_111973, whole genome shotgun sequence.
ACCESSION   DASZOB010000087 DASZOB010000000
VERSION     DASZOB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111973"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VG709_01380"
     CDS             complement(<1..225)
                     /locus_tag="VG709_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018221390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC31763.1"
                     /translation="MNEAAVARSRRLVPPLLRESLEFRRFFTGETVSLVGDQIGAIAL
                     PLVAVLALDATPAQMGYLTAAALAPNLFFSL"
     gene            complement(222..812)
                     /locus_tag="VG709_01385"
     CDS             complement(222..812)
                     /locus_tag="VG709_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWC31764.1"
                     /translation="MGQRTLELTDPRALRAVAHPVRLSLVGLLRREGALTATEAGHLL
                     GESAANCSFHLRQLAKHGLVEEAGGGRGRERPWRATAWLTSWPNVADDPDLAAALGAL
                     NAVVVDRYFAQVRGWLAARDDDPPEWQEASTFGDRILYVTPEELAQVKREVEENLARY
                     EERAARPELRPEGTRAVTWIQFAFPAPPADGADTSG"
     gene            complement(867..1250)
                     /locus_tag="VG709_01390"
     CDS             complement(867..1250)
                     /locus_tag="VG709_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC31765.1"
                     /translation="MFRDTKAFSSFSVDDLQRAKEFYGETLGLDVVESPEGLELHIAG
                     STPVFVYPSHNYTAPKHTVLNFPVDDIEAAVDELAARGVRMENYDLPDLKTDERGIFR
                     GESGPRAIAWFKDPAGHVLSVLQTE"
     gene            1470..>1981
                     /locus_tag="VG709_01395"
     CDS             1470..>1981
                     /locus_tag="VG709_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWC31766.1"
                     /translation="MPAFEALLDAWDGETVVIRRDRESGAWIFICVHSTRRGPAAGGT
                     RMKVYRAPAEALEDAMRLSAAMTRKLAVAGLPFGGGKAVIAPAEIPSGEPRRALFLRY
                     GHLVASLGGSYRTSSDMNTGEADMDVVGERTEYVFGRSVGAGGSGSPAPPTAVGVYYG
                     IRASLAHVFGS"
BASE COUNT          305 a          658 c          692 g          326 t
ORIGIN      
        1 gagggagaag aagagattcg gcgccagcgc ggctgcggtc aggtagccca tctgcgcggg
       61 cgtcgcgtcg agcgcgagga ccgcgacgag cggaagcgcg atagcgccga tctggtcgcc
      121 gacgagcgag actgtctcgc ccgtgaagaa gcggcggaac tccaggctct cgcgaaggag
      181 cggcgggacg agccgccggc tccgagcgac ggccgcttcg ttcatcccga cgtgtctgcg
      241 ccgtctgcgg gcggagcggg aaacgcgaat tggatccacg tcacggcccg cgtgccctcg
      301 gggcgaagct cgggtcgagc ggcgcgctcc tcgtagcggg cgaggttctc ctcgacctcg
      361 cgcttgacct gcgcgagttc ctctggagtg acgtaaagga tcctgtcgcc gaaggtcgag
      421 gcttcctgcc attcgggcgg gtcgtcgtcc cgggcggcga gccagcccct cacctgcgcg
      481 aagtaccggt cgacgacgac cgcgttcagc gcgccgagcg ccgcggcgag atccggatcg
      541 tcggcgacgt tcggccagct cgtgagccac gcagtcgcgc gccacgggcg ctcgcggccg
      601 cggccgcctc ccgcttcctc gacgagcccg tgcttggcga gctgacggag gtgaaacgag
      661 cagttagccg cactctcgcc cagcagatgg ccggcttctg ttgccgtcag cgccccttcg
      721 cggcgcagaa gtcccacgag cgaaagccgc acggggtgtg cgaccgccct cagggcgcgc
      781 ggatccgtga gctcgagcgt gcgctgaccc accgtctaaa catacctttc gaaacagttc
      841 ttttcaaggg tcccgactcg gaggggctac tccgtttgga gtaccgaaag aacgtgaccg
      901 gccgggtcct tgaaccaggc aatggcccgc gggccggact cgcctctgaa gattcctcgc
      961 tcgtcggtct tcagatcggg caggtcgtag ttctccatgc gaacgcccct cgcggcgagc
     1021 tcgtccaccg ccgcctcgat gtcgtccacg gggaagttga ggaccgtgtg cttcggcgcg
     1081 gtgtagttat gcgagggata gacgaacacc ggcgtgctgc cggcgatgtg gagctccaat
     1141 ccctccggac tctcgacgac gtcgagaccc agcgtctcgc cgtagaactc cttcgcccgc
     1201 tgcaggtcgt cgaccgagaa gctgctgaat gccttcgtgt ccctgaacat atgccaccac
     1261 cttggttggg tcttgggttg acctgtctat gtattgaccg gcccgagtcc ccgaagtcat
     1321 cggtgtggag ccgctcgtcc ggcaggcaac ggggcgcctc gccgacggtg acagccggat
     1381 cttgttctcg cgcgcgggcg ggcacgacga ggaaagcaca ggggggcggg tacttgaacg
     1441 tcgcgctcgc ggcgtgatcg aatcgcagcg tgccggcgtt cgaagcgttg ctcgatgctt
     1501 gggacggcga gacagtcgtc atccgccgtg atcgggagag cggcgcctgg atcttcatct
     1561 gcgtccattc gacgcgccga ggtcccgccg caggcggcac gcgaatgaag gtgtaccggg
     1621 cgcccgccga ggcgctggaa gacgccatgc ggctctccgc cgcaatgacc cggaagctcg
     1681 ccgtcgcagg gcttcccttc ggcggcggca aagcggtgat cgcgccggca gagatcccga
     1741 gcggagagcc gcgccgcgcg ctctttctgc gctacggcca cctcgtcgcg tcgttgggtg
     1801 gcagctaccg gacgagctcg gacatgaaca cgggcgaggc cgacatggac gtcgtcggcg
     1861 agcgaaccga gtatgtcttt ggtcgctcgg tcggagccgg cggctcgggc agtcccgccc
     1921 cgccgaccgc ggtcggcgtc tattacggca tccgcgcgag cctggctcac gtcttcggct
     1981 c
//
LOCUS       DASZOB010000088         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_62573, whole genome shotgun sequence.
ACCESSION   DASZOB010000088 DASZOB010000000
VERSION     DASZOB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62573"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VG709_01400"
     CDS             complement(<1..605)
                     /locus_tag="VG709_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HWC31767.1"
                     /translation="MSVVRPGAAAAQRVTTGAIRAKVPLRISFSGGGTDVMPYPKERG
                     GCVLSATIDLYAFASLRPRDDADYQVFSLEGDSARYSSEGDLVFDGHLDLVKACLRHM
                     APDAGLDLHLFCDAPPGSGLGSSSALVVAMLAAVAEHTHTPLTPYDLSRRAYEVERVD
                     LRQAGGMQDQYACAFGGFNFIEFLDVDRVVVNPLRIPPDVA"
     gene            complement(602..1747)
                     /locus_tag="VG709_01405"
     CDS             complement(602..1747)
                     /locus_tag="VG709_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWC31768.1"
                     /translation="MKVLVVTNDFPPKVGGVNYYVANLLREFPPGEVTVFAPDWPGAE
                     SFDRAFTQKVVRWPVQSIYPTPRVRDAVVELVSEARPDILLFGSTLPFALLGRHVRRR
                     LGVPYAAFAHGVEIWAAKVPVATRVLRVLAEDAALFTCVSQWQADLLQKAIGPRARTE
                     LLPPGIDAERFHPGVSTAAVRRRHDLGEGPVICCVSRLVLRKGQDQVIRALPRIAEEF
                     PGVRFLVVGSGPDYARLRALAERKKVEDRVVFAGEVPYDELPMYFRAGDVFAMPNRTR
                     KWGLEVEAFGAVFLQAAAVGRPSVAGDSGGAPAAVWRGETGLVVDGTDRDAVGEAILS
                     LLRAPERAEKMGALGADRVRRDLTWKALSERLRDLLADALGSLPSES"
     gene            2218..3291
                     /locus_tag="VG709_01410"
     CDS             2218..3291
                     /locus_tag="VG709_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31769.1"
                     /translation="MTTRKMLGAAAAMLLVVAMGVGPAAGQTSTDVSLAALTGSRTLI
                     VTTPTGANLDGGELDLGTSHTGGFVTSVADVNYDHVGYQVSSTLSNLYLFAGSFDCNK
                     RVDSDDLAVSFPIDAQVHDIGAIVNGLVDVEGTVNGGLATLLDPTGAINTVLTTAGLD
                     PLAGRPLVAGDLTAQLEDTLNGVFDGADSLLPVNLSNGPNGSFAEPAAHPNCGGGAGT
                     PTSRLLMSGEASGVGGFVQGLIADLEALVDGVSLDGLVSGGVVDAAAAQAAAAAAVQD
                     LEDAINAELAGLSLAPIALPQTSDVVDLVLERLIGALDLANLIGQTGLGVSLPNLDAT
                     PDSAPVSGVYKGRMVVTLVDVPS"
     gene            3278..>3922
                     /locus_tag="VG709_01415"
     CDS             3278..>3922
                     /locus_tag="VG709_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31770.1"
                     /translation="MSRADDARRGRAAARPAARLALTVLAVAAFVVASPGSAGALETD
                     RFGVEPYPLFVEGEVRRSFQVSLDPGGTLTDAVRLWNKTRKPVTVRLYGAGVQVADGQ
                     YSVAAYEARDAGAGGWLDPDATEVRLPPNEERIVSFTVSAPPVLPREGQTVALVAESD
                     TGIEAEGVDVVARLAMLVDVRPEPSGLAGISWWVWLAVALIVVATGLGVWQRVRR"
BASE COUNT          575 a         1381 c         1418 g          548 t
ORIGIN      
        1 tgcgcgacgt cgggggggat ccgtagcggg ttgacgacga cgcggtcgac gtccaggaac
       61 tcgatgaagt tgaagcctcc gaacgcgcac gcgtactggt cctgcatccc gccggcctgg
      121 cgcaggtcga cgcgctcgac ctcgtacgcg cgtcgcgaga ggtcgtacgg cgtcagcggc
      181 gtgtgcgtgt gctcggcgac ggcggccagc atcgcgacga cgagcgcgga cgatgagccg
      241 aggcccgacc ccggcggcgc gtcgcagaac agatgcaggt ccagccccgc gtcgggcgcc
      301 atgtggcgga ggcaggcctt gacgagatca aggtggccat cgaagacgag gtcgccctcg
      361 ctcgaatacc gagccgagtc gccttcgagc gagaagacct ggtagtcggc gtcgtcgcga
      421 ggccgcaacg acgcgaacgc gtagaggtcg atcgtcgccg agagcacgca tccgccgcgc
      481 tccttgggat acggcatcac gtcggtgccg ccgcccgaga agctgatccg gagcggcacc
      541 tttgcgcgga tcgcgccggt cgtgacgcgc tgcgccgcag cggcgcctgg acgtacgacg
      601 ctcacgattc cgagggtagc gaaccgagcg cgtccgcgag aaggtcgcgc agccgctcgg
      661 agagcgcctt ccatgtgagg tctcggcgca cgcggtcggc gccgagcgcg cccatcttct
      721 ccgcccgctc gggggcgcga agcaacgaga ggatggcctc cccgacggcg tcccggtcgg
      781 tcccgtcgac cacgagcccg gtctcacccc tccacacggc cgccggcgcg ccgccggagt
      841 cgccggcgac gctggggcga ccgaccgctg ccgcctgcag gaacacggcc ccgaacgcct
      901 ccacctcgag cccccacttc ctcgtgcggt tcggcatcgc gaacacgtcg ccagcgcgga
      961 agtacatcgg gagctcgtcg tacgggacct cgccggcgaa cacgacgcga tcctccacct
     1021 tcttccgctc cgcgagtgcg cgcagccttg cgtagtcggg gccggagccg acgacgagga
     1081 agcgaacgcc cgggaactcc tccgcgatgc gaggcagcgc gcggatcact tgatcctgcc
     1141 ccttgcggag gacgagccgc gagacgcagc agatgacggg gccttcgccg aggtcgtgcc
     1201 gcctgcggac ggccgccgtc gacactccgg ggtggaacct ctccgcgtcg atgccgggcg
     1261 ggaggagctc ggtcctggcc cgcgggccga tcgccttctg cagcaggtcg gcctgccact
     1321 gcgacacgca tgtgaacagc gccgcgtcct ccgcgagtac gcgcagcacg cgcgtcgcga
     1381 ccgggacctt cgcggcccag atctcgacgc cgtgcgcgaa cgccgcgtac gggacgccga
     1441 gacggcgccg cacgtggcgc ccgaggagcg cgaagggaag cgtggagccg aacagcagga
     1501 tgtcgggccg cgcctcggag acgagctcta cgacggcgtc gcgcacgcgc ggcgtcgggt
     1561 agatgctctg caccggccag cggacgacct tctgcgtgaa cgctcggtcg aacgactccg
     1621 cgccgggcca gtccggagcg aacaccgtca cctcgcccgg cgggaactcc cggaggaggt
     1681 tggcgacgta gtagttgacg ccgcccacct ttggcgggaa gtcgttggtg acgacgagca
     1741 ccttcacggc cgcaccccct ttacccgacg cgacgatcgc ctacccggtc gaggagccgc
     1801 cggatgatac cgccgccggt gcgtgcgcga tcggggcgtg gtgatccgcc cgctcgtcgc
     1861 gcgcgtcccg aaggtccgtc tttcgcctag aagatgactc ggtcgggcta ttcaccggcc
     1921 cgtcggcggt cccgatactc atctcagaca caggggtccg acgcccgccg ccacatgccg
     1981 gcgggcgcgg caggcggaag gagaaggcga cggaccagct cgatgcggga ggagcattcg
     2041 acgtcgcgct cacggttgag cgcgcggcgc cgccaccgac cggcgacaca cccgagctcg
     2101 ccttcaccgg cgcaccccta ctggtgctga tcgcgcttct catggtcctc gtcgcgagcg
     2161 gcttgaacgt gctcgcctcg gcgaggcgtc gagaggcgta ggggagagga gaagaagatg
     2221 accacaagga agatgctcgg agccgccgcc gcgatgctcc tggtcgtggc gatgggcgtc
     2281 gggcccgccg ccggccagac atcgactgac gtctcgctcg cggcgctcac cggcagccgg
     2341 acgctcatcg tcaccacacc gacgggcgcg aacctcgacg ggggcgagct cgatctcggg
     2401 acgtcgcaca ccggagggtt cgtgacctcg gtcgccgacg tgaactacga ccacgtcgga
     2461 taccaggtgt cgtcgacgct ctcgaacctg tacctgttcg ccggctcgtt cgactgcaac
     2521 aagagggtgg actccgacga cctcgcggtg tcgttcccga tcgacgcaca ggtacacgac
     2581 atcggggcga tcgtgaacgg gctcgtcgac gtcgagggga ccgtgaacgg cggcctcgcg
     2641 acgctgctcg acccgaccgg cgcgatcaac accgtgctga cgaccgcggg tctcgacccg
     2701 ctcgccggcc gtccgctcgt cgccggcgac ctgacggcgc agctcgaaga cacgctgaac
     2761 ggggttttcg acggtgcgga ctcgctcctg ccggtcaacc tctcgaacgg tccgaacggg
     2821 tccttcgccg agccggccgc gcaccccaac tgcgggggcg gagccggaac gcctacgtcg
     2881 cggctcctca tgagcggcga ggcatccggg gtaggcgggt tcgtgcaggg tctcatcgcg
     2941 gacctcgagg cgctcgtcga cggtgtgtcg ctcgacggcc tcgtctcggg cggtgtcgtc
     3001 gacgcggcgg ctgcgcaggc cgcggccgcg gcggcggtgc aggacctcga ggacgcgatc
     3061 aacgcggagc tcgccgggct ctcgctcgcg ccgatcgcgc tgccgcagac gagcgacgtc
     3121 gtcgacctcg tgctcgagcg gctgatcggg gctctcgacc ttgcgaacct catcggccag
     3181 acgggcctcg gcgtgtcgct cccgaacctc gacgcgaccc ccgactccgc gccggtctcc
     3241 ggcgtgtaca agggtcggat ggtcgtgacg ctggtggatg tcccgagctg acgacgcgcg
     3301 acgtggacgg gcggccgcga gaccggccgc ccgtctcgcg ctgacggttc tcgccgtcgc
     3361 ggcgttcgtc gtcgcctcgc cggggtcggc gggcgcgctc gagaccgacc ggttcggggt
     3421 ggagccgtac ccgctcttcg tcgagggcga ggtccgtcgc tcgttccagg tctcgctcga
     3481 tccgggcggc acgctcaccg acgccgtgcg gctgtggaac aagacgcgga agccggtgac
     3541 cgtccggctc tatggtgcgg gcgtccaagt tgcggacggc cagtacagcg tcgccgcgta
     3601 cgaggcgcgc gacgccggcg ccgggggatg gctcgatccc gacgcgaccg aggtccgcct
     3661 gccaccgaac gaggagcgca tcgtgtcgtt cacggtgagc gcacctccag tccttccgcg
     3721 ggaggggcag accgttgccc tcgtcgccga gagcgacacg gggatcgagg cagagggggt
     3781 cgacgtcgtg gcccggctcg cgatgctcgt ggacgtccgg cctgagccgt ccgggctcgc
     3841 cgggatctcg tggtgggtgt ggctcgccgt cgcgctgatc gtcgtggcga cggggctcgg
     3901 ggtatggcag cgcgtccgcc gc
//
LOCUS       DASZOB010000089         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_1995,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000089 DASZOB010000000
VERSION     DASZOB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="purN"
                     /locus_tag="VG709_01420"
     CDS             <1..421
                     /gene="purN"
                     /locus_tag="VG709_01420"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWC31771.1"
                     /translation="DVALLELLRDRGIEVVCLAGYMRILRPAFVRAMRPRILNVHPSL
                     LPSFPGAHAVRDALAWGVKVTGATVHLVDEEVDHGPVIAQEAVPVLDDDDEETLHARI
                     RAVEHRIYPEAVRLVLDGRLALEGRRTVVRAPAAPRR"
     gene            418..1977
                     /gene="purH"
                     /locus_tag="VG709_01425"
     CDS             418..1977
                     /gene="purH"
                     /locus_tag="VG709_01425"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWC31772.1"
                     /translation="MTAATSPGGRLSVRRALLSVSDKAGVAELATELASLGVEIVSTG
                     STAEAIEAAGVSVTTVADVTGFPEMMGGRVKTLHPAIHAGILADRHDEGHMREIEERG
                     IAPFDLVVVNLYPFESTVAAGGTRDAVIEQIDIGGPALVRAAAKNFRSVAVVVRPDRY
                     GELLAAVRDGGGVDERLRLRLAEEAFALVAVYDAAIASWFAELVGDDEIPRSLTLGLQ
                     RTSVLRYGENPHQRAALYRAALSHGPLGGADVLQGKEMSFNNWLDAEAARALASALDS
                     PAAVIVKHNNPCGAAVANTAADAYRAAYECDPVSAFGGVVAFNAPVDAAATAAMADVF
                     TEVVVAPAFTDGALSALTGRKNLRVVRAAARPERALDVRAIEGGALVQDPDEVTERRD
                     AFEVVTRRAPDDDEWEDLLFAWRVCARVKSNAIVLARLRRTVGVGAGQMSRVDAVDIA
                     CRKAGERAAGARMASDAFFPFRDGVDRAAEAGVTAVIHPGGSMRDEEVVEAADAHGMA
                     MVLTGRRHFRH"
     gene            2089..3213
                     /locus_tag="VG709_01430"
     CDS             2089..3213
                     /locus_tag="VG709_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWC31773.1"
                     /translation="MRRRIWHVAVLALTLAVVAAACTREQPAPEGQGGGGGTEAGGAQ
                     QGVNLLAEIQERGTLRCGVNNTVPGFGFETAEGTIEGFDVDFCIAIAAALFGTDSPEE
                     GTHYELVPIDADNRFTALRAGEYDVLVRNTTWTSSRDGTEGVQFTKVNFYDGQAMIAP
                     AANVSSIEDLDGATICVTAGTTTELNLADYFREQGLDFEPLTLEDYDQIFQAFRQERC
                     DAVTGDRSALASLAPTWSEDIGELTILEDVMSKEPLTPAVLEGDANWHDVVFWVTNGL
                     ILAEELGVTQDNVQQEAQNPSTPGIAALLGAPFAEDEEVNVAHGLELDDTFMQNAIAA
                     VGNYGEIFDRNIGPDTEIGLERGLNALWTEDGLQYAIPFR"
BASE COUNT          474 a         1096 c         1213 g          446 t
ORIGIN      
        1 cgacgtcgcg ttgttggagc tcctccgaga ccgcgggatc gaggtcgtgt gcctcgccgg
       61 ctacatgcgg atcctcaggc cggcgttcgt ccgcgcgatg aggccgcgga tcctgaacgt
      121 ccatccgtcg ctgctgccgt cgttccccgg ggcacacgcc gttcgtgacg cgctcgcgtg
      181 gggcgtcaag gtgaccggcg cgaccgtcca cctcgtcgac gaggaggtcg accacgggcc
      241 ggtcatcgcg caggaagcgg tcccggtcct ggacgacgac gacgaggaga cgctgcacgc
      301 gcggatccga gccgtcgagc accgcatcta tccggaggcc gtccggctcg tgctcgatgg
      361 gcgcctcgcg ctggagggaa ggcggaccgt cgtgcgcgct ccggccgcgc cgcggcgatg
      421 acggcggcga cctcgccggg cggccggctc tcggtccggc gcgcgctgct gtcggtctcc
      481 gacaaggccg gcgtcgcgga gctcgcgacc gagctcgcct cgctcggtgt ggagatcgtc
      541 tcgaccggct ccacggccga ggcgatcgag gcggccggcg tctccgtcac cacggtcgcc
      601 gacgtgaccg gcttccccga gatgatgggc gggcgagtca agacgctcca cccggcgata
      661 cacgcgggga tcctcgcgga caggcacgac gaggggcaca tgcgcgagat cgaggagcga
      721 ggcatcgccc cgttcgacct cgtcgtcgtc aacctctatc cgttcgagag caccgtcgcg
      781 gccggcggga cgcgcgatgc ggtgatcgag cagatcgaca tcggcggccc ggcgctcgtc
      841 cgcgcggccg cgaagaactt ccggtcggtc gcggtcgtcg tgcggcccga ccggtacggc
      901 gagctgctcg ccgcggtccg cgacggcgga ggcgtcgacg agcggctccg gctccggctc
      961 gccgaagagg cgttcgcgct cgtggccgtg tacgacgccg cgatcgcctc gtggttcgcg
     1021 gagctcgtcg gcgacgatga gatcccgcgc tcgctgacgc tcgggctcca gcgcacttcc
     1081 gtcctccggt acggcgagaa cccgcaccag cgtgcggctc tctaccgcgc ggcgctctcg
     1141 cacgggccgc tcggaggtgc cgacgtgctc cagggaaagg agatgtcgtt caacaactgg
     1201 ctcgacgccg aggcggcacg cgcgctcgcc tccgccctgg actctccggc cgccgtgatc
     1261 gtgaagcaca acaacccctg cggagccgcc gtcgccaaca ccgcagcgga cgcgtatcgc
     1321 gccgcgtatg agtgcgaccc ggtctcggcg ttcggaggcg tcgtggcgtt caacgccccg
     1381 gtcgacgccg cagcgaccgc cgcgatggcg gacgtcttca cggaggtcgt cgtggcgccc
     1441 gctttcacgg acggggcgct gtccgcgttg accggacgga agaacctccg cgtcgtgcgt
     1501 gccgcggccc gccccgagcg ggcgctcgac gtccgcgcga tcgagggcgg ggcgctcgtg
     1561 caggaccccg acgaggtgac ggagcggcgc gacgcgttcg aggtcgtgac gcgacgcgcg
     1621 cccgacgacg acgagtggga ggacctgctg ttcgcgtggc gcgtgtgcgc acgggtcaag
     1681 tcgaacgcga tcgtcctcgc gaggctgcgg cggacggtcg gcgtgggcgc cggccagatg
     1741 agccgcgtcg acgccgtcga catcgcgtgc cgcaaggccg gcgagcgcgc ggcgggtgcg
     1801 cggatggcct ccgacgcgtt cttcccgttc cgcgacggcg tcgaccgcgc ggctgaggcc
     1861 ggcgtgacgg ccgtcatcca ccccgggggc tcgatgaggg acgaggaggt cgtcgaggcc
     1921 gcggacgccc acgggatggc catggtgctc acggggcgcc gccacttccg ccactaaggc
     1981 gcatacggtg cgtttggccc cacgcggacc gtccgactat cctcgcccct gagctagggg
     2041 atcggccgtc cctgcgacgg ccagtcgtcg aagggaagga ggcacgagat gcggaggcgc
     2101 atctggcatg tggccgtgct ggcgctcacg ctcgcggtgg tcgcggcggc ctgcacgcgc
     2161 gagcagcccg cgccggaggg tcagggcggc gggggcggta ccgaggccgg aggtgcgcag
     2221 cagggcgtga acctgcttgc ggagatccag gaacgcggga ccttgcggtg cggcgtcaac
     2281 aacaccgtcc cggggttcgg gttcgagacc gcggagggca cgatcgaggg cttcgacgtg
     2341 gacttctgca tcgccatcgc ggcggccctg ttcgggacgg actcgccgga ggagggcacg
     2401 cactacgagc tggtgccgat cgacgcggac aaccggttca ccgccctgcg ggcaggcgag
     2461 tacgacgtcc tcgtgcggaa caccacgtgg acgtcgagcc gcgacggaac ggagggcgtg
     2521 cagttcacga aggtgaactt ctacgacgga caggcgatga tcgcgccggc cgcgaacgtg
     2581 agctcgatcg aggacctcga cggggcgacg atctgcgtca cggcaggcac cacgaccgag
     2641 ctgaacctcg ccgactactt ccgtgagcag gggttggact tcgagccgct cacgctggag
     2701 gactacgacc agatcttcca ggccttccgg caggagcggt gcgacgccgt gaccggcgac
     2761 cgctcggcac tggccagcct ggcgccgacc tggtcggagg acatcggcga gctgacgatc
     2821 ctcgaggacg tcatgtcgaa ggagccgctg acgccggcgg tgctcgaggg tgacgcgaac
     2881 tggcacgacg tcgtgttctg ggtgacgaac gggctcatcc tggccgagga gctcggcgtg
     2941 acgcaggaca acgtccagca ggaggctcag aatccctcga cgcccgggat cgccgcgctg
     3001 ctcggcgcgc cgttcgccga ggacgaggag gtgaacgtgg cgcacggcct ggagctggac
     3061 gacacgttca tgcagaacgc gatcgccgcg gtcgggaact acggggagat cttcgaccgc
     3121 aacatcgggc cggacacgga gatcgggctc gaacggggcc tgaacgcgct gtggaccgag
     3181 gacggtctgc agtacgcgat cccgttccgg tagaaccagc gttcggcgt
//
LOCUS       DASZOB010000090         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_172538, whole genome shotgun sequence.
ACCESSION   DASZOB010000090 DASZOB010000000
VERSION     DASZOB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172538"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..983)
                     /gene="gcvPB"
                     /locus_tag="VG709_01435"
                     /pseudo
     CDS             complement(1..983)
                     /gene="gcvPB"
                     /locus_tag="VG709_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202224.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
     gene            complement(1034..2350)
                     /gene="gcvPA"
                     /locus_tag="VG709_01440"
     CDS             complement(1034..2350)
                     /gene="gcvPA"
                     /locus_tag="VG709_01440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWC31774.1"
                     /translation="MRFAPHTDTDVREMLDELGLSSVEDLYAHIPADVSLDRPLDIPS
                     GVSELELLEDLRRLAARNRHADDLVCFAGGGAYDHYVPSVVWALAGRSELQTSYTPYQ
                     PELSQGVLQATYEYQSMICELTDLEVSNASLYDGPTALMEAVNLSRAATGRDRVLVAG
                     TLDARHAQTLEAYGRGAGYTPEILEADGGRTRAPEMRDDLACVVVQHPNAFGILEPAR
                     EIFAAARRGGARAIHVFDPLALGVLASPGELGADIAVAEGHVLGNHLNLGGPYLGVMA
                     ARLQDVRRMPGRIAGETLDADGHAGYVLTLQAREQHIRREKATSNICTNQTLMAIAAA
                     VYLAWLGPQGLREVGEASAAKARYAADRLTEIDGVRLAFPGVPFFKEFAVSFDGASPN
                     EVRDRLVELGLLAGPVVDGSLLVACTERRTREEIDLLAAATREALS"
     gene            complement(2415..2822)
                     /locus_tag="VG709_01445"
     CDS             complement(2415..2822)
                     /locus_tag="VG709_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31775.1"
                     /translation="MSATAEATHPRDSDPSHHTEAGGHKSDTTPFAHASERQFAKLLD
                     FYQIEWEYEPRSFDLEWDEEGNVVQRFTPDFYLPAWDLYIEITTMNQKLVTKKNRKVR
                     RLKELYPEINCRIFYQRDYLSLVTKYGLEDRSG"
     gene            complement(2819..>2999)
                     /locus_tag="VG709_01450"
     CDS             complement(2819..>2999)
                     /locus_tag="VG709_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019548779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31776.1"
                     /translation="HGMTLMSDGRKIYAAESPGEVMALLQRGQGVFAIAVDKVWTDVE
                     GSLRRTAKAARAGGS"
BASE COUNT          429 a         1054 c         1053 g          463 t
ORIGIN      
        1 cgaaccagca ccgcgaagtt gccgtggaag ccgtggatcc gcccgatcga atccggacgg
       61 tcctcgtccc agcggaacgt cccgtcctcg ctcttcgaca gcaccggcga cggaaggtac
      121 ggcgtgaggc gctcggtgac ggccaccgga ccggcacccg gtcctccccc gccgtggggc
      181 gtcgcgaacg tcttgtgggt gttcatgtgc acgatgtcga accccatgtc gccgggccgc
      241 acccggccga ggatcgcgtt gaggttcgcg ccgtcgtagt acaggagccc cccgacgtcg
      301 tggatcgtgc gcgcgagctc caggatgtcc tgctcgaaca gcccgagggt gttcgggttc
      361 gtgatcatca gtcccgcgac gtcctcgtcg acgagcttct cgagcgccga caggtcgacg
      421 aggcctcggg cgtccgacgg caccgtggcg acctcgtagc ccgccatccg caccgaagcc
      481 gggttcgtgc cgtgcgccga gtcggggatc acgacgcggc gcctcgcgtt cccctgcctc
      541 gcgtggtagg cgcgcatgat cagcaggccg gtgagttcgc ccgaggcccc cgcgggcggc
      601 tggcacgtcg cccgcgccat gccggtcacc tagcacagcg ccctctcgag tcgccacagc
      661 agctcgaggg cgccttggac cgtctgatcc ggttggtggg gatggagccg ctggaacccc
      721 gggatcgcgg cggccgcgtc ggcgaccttc gggttgtact tcatcgagca cgacccgagc
      781 ggatagaagc cggtgtcgac gccgtagttc cgctgcgcga gccgcgtgaa gtggcggacg
      841 aggtcccgct cccccacctc ggggagcgac gcgggctcgg cgcggcggtg ctcctcggga
      901 acgtcggccg ccgggacgtc gagcttcggg aacgacgacg cgcgccgccc ggcacgcgag
      961 agctccgcaa gcgtccgcgg caggtcgggg gcgcccgtcc ggaccggcgc cccgacgcgg
     1021 atgtcgccct cggtcacgac agcgcctctc gggtggcggc ggcgagcagg tcgatctcct
     1081 cgcgcgtcct tcgctcggta caggcgacga ggagcgagcc atcgaccacg ggccccgcga
     1141 ggagcccgag ctcgacgagc cggtctcgaa cctcattcgg cgacgccccg tcgaacgaca
     1201 ccgcgaactc cttgaagaac ggcacccctg ggaaggcgag tcggacgccg tcgatctcgg
     1261 tgaggcggtc cgcggcgtac cgcgccttcg cggcggacgc ctcgccgacc tcgcgaagac
     1321 cctgagggcc cagccatgcg aggtagacgg ctgccgcgat cgccatcagc gtctggttcg
     1381 tgcagatgtt cgacgtcgcc ttctcacgac ggatgtgctg ctcgcgcgct tggagcgtca
     1441 acacgtagcc ggcgtggccg tcggcgtcga gcgtctcccc cgcgatccgt ccgggcatgc
     1501 gtcgcacgtc ctggaggcga gccgccatga cgccgaggta cgggccgccg agattcaggt
     1561 ggttcccaag gacgtgcccc tccgcgaccg cgatgtcggc gccgagctct cccggagacg
     1621 cgagcacgcc gagcgcgagc ggatcgaaga cgtggatcgc gcgcgcgccg ccacgccgag
     1681 cggccgcgaa gatctcgcgc gccggctcga ggatcccgaa ggcgttcggg tgctgaacga
     1741 cgacgcaggc gaggtcgtcc cgcatctcag gcgcgcgcgt gcggccgccg tccgcctcca
     1801 ggatctccgg cgtgtacccg gctccgcgac cgtacgcctc gagggtctgc gcgtgccgcg
     1861 cgtcgagcgt ccctgcgacc agcacgcggt cgcggccggt cgcggcgcgc gagaggttca
     1921 ccgcctccat gagcgcggtc ggcccgtcgt agagcgacgc gttcgagacc tcgagatcgg
     1981 tgagctcaca gatcatcgac tggtactcat acgtggcctg gagcacaccc tgcgagagct
     2041 cgggctggta gggcgtgtac gacgtctgca gctccgagcg cccggccagc gcccacacga
     2101 ccgaggggac gtagtggtcg tacgcgccgc cgcccgcgaa gcacacgagg tcgtcggcgt
     2161 ggcggttccg cgcagccagc ctgcggaggt cctccaggag ctcgagctcc gagaccccgg
     2221 acgggatgtc gagcgggcgg tcgagcgaga cgtcggccgg gatgtgcgcg tagaggtcct
     2281 cgaccgacga gagcccgagc tcgtcgagca tctcgcggac gtccgtatcg gtatgcggag
     2341 cgaagcgcat gttggtcagc tcgtctgggc cggggccggc ggcatcctac cgcaccgccg
     2401 ctctccgacc ggcgctatcc gctccggtcc tcgaggccgt acttcgtgac gagcgacagg
     2461 tagtcgcgct ggtagaagat gcggcagttg atctcggggt agagctcctt cagtcggcgc
     2521 acctttctgt tcttcttcgt cacgagcttc tggttcatcg tggtgatctc gatgtacagg
     2581 tcccaggccg gcaggtagaa gtcgggcgtg aaccgctgga cgacgttccc ctcctcgtcc
     2641 cactcgagat cgaacgagcg aggctcgtac tcccactcga tctggtagaa gtccaggagc
     2701 ttcgcgaact gccgctcgct cgcgtgcgcg aagggcgtcg tgtcgctctt gtgtccgccg
     2761 gcctcggtgt gatgggacgg gtcgctgtcg cgcggatggg ttgcttccgc cgtggcgctc
     2821 aagaccctcc ggcccgagcc gccttcgcag tccgacggag cgagccctcg acgtcggtcc
     2881 agaccttgtc gacggcgatc gcgaagacgc cctgaccccg ctgtaacagg gccattacct
     2941 caccgggcga ctcggccgcg tagatcttcc ggccgtccga catgagcgtc atcccgtgc
//
LOCUS       DASZOB010000091         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_41471, whole genome shotgun sequence.
ACCESSION   DASZOB010000091 DASZOB010000000
VERSION     DASZOB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41471"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VG709_01455"
     CDS             <1..597
                     /locus_tag="VG709_01455"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWC31777.1"
                     /translation="NLGRVGFLAKVEAAGLEDALDQIWRGEYGVQKRMRLEATITRTG
                     GATERHACVNDVAIARGAQARVLHLAVDVSGTHLATYVADGVVVATPTGSTAYSFSAG
                     GPILDPSLRNLVVTPIAAYLTAIRSVVVGAEHKITVTRLNAGDPGVVSLDGQLDVPLE
                     EGDRVDVRALDEPLKLIEPHGSVPFYDLLRTKATLLPR"
     gene            626..>1879
                     /locus_tag="VG709_01460"
     CDS             626..>1879
                     /locus_tag="VG709_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC31778.1"
                     /translation="MALVELTVRNLAVIESVRISLAPGFNVVTGETGAGKSLVIDALA
                     LALGGRASADQVRAGSASAHVEAIFDAPATTAAEGDDPLADLLDASDDVVIVRREVTA
                     EGRSVIRLNDRAVTVGTLAGVGDRLAEIHGQHEQQRLLKPEHQMSLLDPFGGHLDLVA
                     AVTEAYRAWRATQARMEELVTDAHELARRLELLEHQAGEIAAARLRVGEDAELETTLR
                     AAQHGEAIVRAATGAVGALRGADDEPAAVVALRSAVASLRAAAAHDERFSEVQGRAEG
                     LAAEAEELARDVEALVAAIDLDPASRERMEERLSLIYDMKRKYGDGTEAILQFGAQAE
                     SQLERLRNQDELRARARDDEAHARDALATASDALAEARRDAATRLADAVNAELPPLGL
                     RGGAFGVDVRDLGEPGLRGTERIEFT"
BASE COUNT          281 a          626 c          716 g          256 t
ORIGIN      
        1 aatctgggac gcgtgggctt ccttgcaaaa gtggaggccg ccgggctcga ggatgcgctc
       61 gaccagatct ggaggggcga gtacggggtg cagaagcgga tgcggctcga agccacgatc
      121 acgcgcaccg gtggggcgac cgagcggcat gcctgcgtga acgacgtggc gatcgcgcgc
      181 ggtgcacagg cgcgcgtcct ccacctcgcc gtcgacgtga gcgggaccca tctggccacc
      241 tatgtcgcgg atggcgtcgt cgtcgcgacg ccgaccggct cgacggcgta ctcgttctca
      301 gcgggcggcc ccatcctgga cccgagcctc cggaacctcg tcgtcacgcc catcgccgcg
      361 tacctgaccg cgattcggag cgtcgtcgtc ggcgcggagc acaagatcac ggtgacgcgc
      421 ctgaacgccg gcgatcccgg ggtcgtgtcc ctcgacgggc agttggacgt gccgctcgag
      481 gagggcgatc gggtcgacgt gcgcgcgctc gacgagcctc tgaagctgat cgaaccacac
      541 gggagcgtcc cgttctacga cctgctgcgc acgaaggcta ccctgcttcc gcggtgacgg
      601 ggatgcagga cggagacgga gggcaatggc actcgtcgag ctgaccgttc ggaacctcgc
      661 ggtgatcgag tctgttcgga tctcccttgc gcccggattc aacgtcgtca cgggtgagac
      721 gggcgcgggg aagtcgctcg tgatcgatgc gctcgcgctt gcactgggcg gccgcgcatc
      781 cgcggaccag gttcgagccg gctctgcttc cgcgcacgtg gaggccatct tcgatgcgcc
      841 cgcgacaacg gccgcggagg gcgacgaccc gctcgccgac ctgctcgacg cgtctgacga
      901 cgtcgtcatc gtgcggcgcg aggtcacggc cgagggccgc tcggtgatcc gattgaatga
      961 ccgtgccgtc acggtcggga ccctcgccgg cgtgggtgat cggctcgcgg agatccacgg
     1021 ccagcacgag cagcagcgcc tgctcaagcc ggagcaccag atgtccctgc tcgacccatt
     1081 cggcgggcac ctggatctcg tcgcggccgt gaccgaggcg tatcgcgcgt ggcgcgccac
     1141 acaggcacgg atggaggagc tggtcaccga tgcgcacgag ctcgcccgcc gcctcgagct
     1201 cctggagcac caggcgggag agatcgcggc cgcgcggctg cgtgtgggcg aagacgcgga
     1261 gctggagacg acgcttcgcg ccgcgcagca cggcgaggca atcgtccggg ccgcgacagg
     1321 agcggtgggg gcgctccgcg gcgcagatga cgagcccgcg gccgtggtgg cgctccgcag
     1381 cgccgtcgcg tcgcttcgcg cggcagcggc gcacgacgag cgcttctccg aggtgcaggg
     1441 gcgcgcggag ggtcttgcgg cagaagcgga ggagctcgcg cgggacgtgg aggcgctcgt
     1501 cgcggcgatc gacctcgacc cggcgtccag ggagcgcatg gaagaacgcc tgagcctgat
     1561 ctacgacatg aagcgcaagt acggtgacgg gaccgaggcg atcctgcaat tcggcgcgca
     1621 ggccgagtct cagctcgagc ggctgcgcaa ccaggacgag ctgcgggcgc gggcgcgcga
     1681 cgacgaggcg catgctcgcg acgcgctcgc gacggcgtcg gacgcgctcg ccgaggcgcg
     1741 gcgggacgcc gccaccaggc tcgcggacgc ggtgaacgcg gagctgcctc cgctgggcct
     1801 gcgcggcggc gcgttcgggg tcgatgtccg cgacctcggt gagcccgggc tgcgtgggac
     1861 ggagcggatc gagttcacg
//
LOCUS       DASZOB010000092         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_122826, whole genome shotgun sequence.
ACCESSION   DASZOB010000092 DASZOB010000000
VERSION     DASZOB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122826"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="VG709_01465"
     CDS             complement(<1..1870)
                     /locus_tag="VG709_01465"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWC31779.1"
                     /translation="MPTGRGSWKIPVLALSVALAAVALGAAPALAQEDKSFRLLRADV
                     AVRVERDGSLRVAETLTFSYSGSFQGAFRDIPLRPGERIDDVSVTEAGRSYRPGASAE
                     IGSSGAPDTFGVARTAEGLRVVWHYRATDETRTFTVGYRFSGLAVAYDDVVDVNLQVW
                     GDEWEVQLDELSASLALPDRATGREYRVFGHPAWVRGVVDRRPAGAELRATSVPPRQF
                     VELRVLFPRELLASTDGAKVRNEPGLDRILAEERADAAAFEEDVAQIQAWRDDLGRYL
                     LLLLALAIAPALLVLAGTWLFFGRERSVAYDREYEQDPPSDLPPALVSPLLRQSTVVG
                     SHEFTATLFDLIRRGRYEARPVTTERSTWAGLRKQQVADLELSIGAADLHEPFERDVA
                     KVVDDVLDGGSERLSRFRDRISEKRTTQAGRFTSFQKHVAEWIDLAGWYDSTGRRVVI
                     GAALLFALAATALLWIGFANLRPGFPRPSDIVLIALGSCAAVNTLVLIVLLAWRVNAA
                     GRRVPLMGERVWRRRTPQTQLEARRWEAFRGYLTDFPRLDVAPPATLEIWERYLVYGI
                     ALGIAERVLQGAQLLMPRELAEASSVYWISGSSDLGSGATSLGIGDLSSGFGSALTP"
     gene            2115..2891
                     /locus_tag="VG709_01470"
     CDS             2115..2891
                     /locus_tag="VG709_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWC31780.1"
                     /translation="MVAVISALIIVLVSLIVTRVATVALILTGLSREVARFQARSALT
                     GTGFTTSESEYVVGHPVRRRILMVLFLVGNAGLVTVIASLAVSFVGAGGARPTLLRAV
                     VLLAGVMIIWLLARSAALDRVLSPLITRLLRRWTDLDVRDYVGLLQLRGEYGVAELQV
                     QEDDWVAGRTLEELKLRDEGIAVLGINRSRKEYVGVPRGDTVVRSGDVIVVYGRAAKL
                     ADLDRRPKGEVGERAHAEAVDDERRREDQGAADAPDQAAG"
BASE COUNT          432 a         1035 c         1071 g          408 t
ORIGIN      
        1 gtggagtgag cgccgacccg aagcccgacg acagatcccc gatcccgagc gaggtcgcgc
       61 ccgaaccgag atcggagctg ccgctgatcc agtagacgga gctggcttcg gcgagctcgc
      121 gcggcatcag cagctgcgcg ccttgtagga ctcgctcggc gatcccgagc gcgatgccgt
      181 agacgaggta ccgctcccag atctcgagcg tcgccggcgg cgcgacgtcg agccgaggga
      241 agtccgtcag gtacccgcgg aacgcctccc agcggcgcgc ctcgagctgt gtctgaggcg
      301 tccgccggcg ccacacgcgt tcccccatga gcggcactcg ccgcccggcg gcgttcaccc
      361 tccacgcgag gagaacgatc aggacgaggg tgttcacggc ggcgcacgag ccgagcgcga
      421 tgaggacgat gtcggacggt ctcgggaacc ccgggcgaag gttcgcgaaa ccgatccaga
      481 gcagcgccgt ggcggccagc gcgaacagca gcgccgcgcc gatgacgacc ctcctccccg
      541 tcgagtcgta ccagcccgcc aggtcgatcc actcggcgac gtgcttctgg aatgacgtga
      601 agcggccggc ttgcgtcgtc cgcttctccg agatcctgtc gcggaaccgc gagagccgct
      661 cggagccgcc gtccaggacg tcgtcgacga ccttcgcgac gtcgcgctcg aacggctcgt
      721 ggaggtccgc ggcgccgatc gagagctcga ggtccgcgac ctgctgcttc cggagcccag
      781 cccacgtcga gcgctcggtg gtgaccggcc gggcctcgta tcgcccgcgg cggatcaggt
      841 cgaagagcgt ggcggtgaac tcgtgtgagc ccacgacggt gctctggcgc aggagcggcg
      901 agacgagcgc gggcggcagg tcgctcgggg gatcctgttc gtactcgcgg tcgtacgcga
      961 cgctgcgctc gcgcccgaag aagagccacg tcccggcgag cacgaggagc gccggagcga
     1021 tcgccagcgc gaggaggagc agcaggtacc gtccgaggtc gtcgcgccac gcctggatct
     1081 gcgccacgtc ttcctcgaac gccgcggcgt cggcgcgctc ctcggcgagg atccggtcga
     1141 gacccggctc attccgcacc ttggcgccgt ccgtggacgc gaggagctcg cgcgggaaca
     1201 gcacacgcag ctcgacgaac tgcctcggcg gcacggaggt tgcgcggagc tcggccccgg
     1261 ccggtctgcg gtcgacgacg cctcgcaccc aggcggggtg cccgaacacg cgatactcgc
     1321 ggccggtcgc gcgatcgggc agcgcgagcg acgcggacag ctcgtcgagc tgcacctccc
     1381 actcgtcgcc ccagacctgg aggttgacgt cgacgacgtc gtcgtaggcg acggcgagcc
     1441 ccgagaaccg gtagccgacc gtgaacgtcc gtgtctcgtc cgtggcgcgg tagtgccaga
     1501 cgacgcggag accctcggcg gtgcgagcca cgccgaacgt gtcgggcgcg ccggaactgc
     1561 cgatctccgc ggatgcgccg ggccggtagc tccttcctgc ctccgtgacc gagacgtcgt
     1621 cgatgcgctc gccggggcgc agcgggatgt cgcggaatgc gccctggaag ctgccgctgt
     1681 aggagaacgt gagcgtctcg gcgacgcgga gcgatccatc ccgctcgacg cgcacggcga
     1741 cgtccgcacg gaggaggcgg aacgacttgt cctcctgcgc gagggcgggc gccgcgccga
     1801 gagcgacggc ggcgagcgcg accgacaggg cgagaacggg gatcttccag ctgcctcgtc
     1861 cggtcggcac gccgtccgaa ctttgggccg catctccaga ggatcgcctc atcgcccgca
     1921 cggtacgccg actcgagcgg acccggaagc tccgcgtctc ttcacgatcg gtgccgccgc
     1981 gctgaagcgg cacgcctcta cgggcggtgc gccgcggttc gggaggtccc ggtagatccc
     2041 gacgccggcc gccgcgcgtt gaaccgcgtc gccgctggga aggaaggaag cgaagcagcc
     2101 gcgggaggac cgacgtggtc gcagtcatct cggcgctcat catcgtgctg gtgtcgctca
     2161 tcgtcacgcg cgtggcgacg gtcgctctga tcctgaccgg gctctcacgc gaggtcgcga
     2221 ggttccaggc gcgctccgcg ctcacgggga cggggttcac gacgtcggag tccgagtacg
     2281 tggtcggtca ccccgtccgt cgccgcatcc tcatggtgct gttcctcgtc gggaacgcgg
     2341 gcctcgtcac ggtcatcgcc tcgctcgcgg tgtcgttcgt cggggccgga ggcgcccgcc
     2401 cgacgctgct ccgcgcggtg gtgctgctcg ccggagtgat gatcatctgg ctgctcgccc
     2461 ggagcgcggc gctcgaccgg gtgctttcgc cgctcatcac gcggcttctc cgtcgctgga
     2521 ccgatctcga cgtccgcgac tacgtcggac tgctgcagct gcgcggggag tacggcgtcg
     2581 ccgagctgca agtccaggaa gacgactggg tcgccggccg cactctcgag gagttgaagc
     2641 tccgcgacga gggcatcgcg gtcctcggga tcaaccgctc gcggaaggag tacgtcggcg
     2701 tgcctcgcgg cgacacggtc gtgcggtcgg gcgacgtcat cgtcgtgtac ggacgcgccg
     2761 ccaagctcgc cgatctcgac cgccgaccca agggcgaggt cggcgagcgg gcacacgccg
     2821 aggcggtcga cgacgagcgc cgtcgggagg accaaggcgc cgccgacgcg ccggaccagg
     2881 ccgcgggata gcggctacga cgcgatctcg aactcgcggc gtcagggcgt cgtctctcgc
     2941 tccagc
//
LOCUS       DASZOB010000093         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_143005, whole genome shotgun sequence.
ACCESSION   DASZOB010000093 DASZOB010000000
VERSION     DASZOB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143005"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VG709_01475"
     CDS             complement(<1..223)
                     /locus_tag="VG709_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31781.1"
                     /translation="MRFGAHVPTPGRLWGAIEYAKAAGCDAFQMFVSNPRAWAPPNHS
                     DDAAREFRARRDASGIGPAFAHSSYLINIA"
     gene            complement(231..2078)
                     /gene="pknB"
                     /locus_tag="VG709_01480"
     CDS             complement(231..2078)
                     /gene="pknB"
                     /locus_tag="VG709_01480"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWC31782.1"
                     /translation="MGRLPPIMLDTTVDGRYVIAARVASGGMGEVYRAHDPVLDREVA
                     IKVLHRSFADDPGFIERFRREARAAAMLSHPNIVAIHDWGETAGTYFMVMEYIRGPNL
                     RTLLLRDGALAPSQAVDVVSQVLAALEHAHRQGLVPRDIKPENVMVRASDGAVKVTDF
                     GLARALADARISQAPGTVTGTVQYLAPEQIEGFPADPRTDLYATGIVLYELLTGQVPF
                     SGETSVAIAYKHLRDRVPPPSRSNPMVPPSLDQVVLSATERDPEGRTRDAYTMRLDVA
                     RVAPDLPPAEPLAELAANAAPREERGPDRAATVTIPRVEPRARRRRRVAMVVGILALL
                     ATLGVAAWAAVMNLRPVIVATPDIEGRSVAEAQRLAEARGLEFAISRERFSSSVPANV
                     VISQRPPAGERVEEGTPLSVVVSRGPELTEVPNVVGDPIDRARRRLRRAELRLSVARA
                     YSDDVPEGRVIEQSLNPGSRPEVGEEITVTVSRGEPPVEVQNVVGLPEGDAVSVLEEQ
                     GLVVEVRRENSSDVRKGLVISQDPPTGNTVPAHSSVTIVVSQGPARFPMPDVVGDSRE
                     AAVEQLEELGLVVAPLEVPGSDGNTVVGQRPSAGTTVRQGDRVRIYIGG"
     gene            2134..2892
                     /locus_tag="VG709_01485"
     CDS             2134..2892
                     /locus_tag="VG709_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31783.1"
                     /translation="MSDARPDEDYQDAFARIERRVEAGDADLSELGFWRLVRKVKVEP
                     ALAAHWADDCGRIDRMAFERRVRPRFPVWLGNALLLVGSIVLVALVPAALAIARGEWL
                     FERPNPLVAGILAVVAAGGLSVTTHDLAHWVVGRLAGIRFIAYFLDGPTRIQPGLKTD
                     YETYLKASPGSRAAMHAAGAIASKIAPFAVFVAVYLPHRASGYGLLPAWSVWTILAIG
                     VVQLLTDILWSTKTSDWKKVRREARLGRAQRHRR"
     regulatory      2923..3037
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3072..3911
                     /locus_tag="VG709_01490"
     CDS             3072..3911
                     /locus_tag="VG709_01490"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWC31784.1"
                     /translation="MTTREVATARDPLVIAGRTFTSRLIVGTGKFGSYDAMRDALTAS
                     GTEMVTVALRRVDLDSTGGPDILQFIDPDRYLLLPNTSGAVDSDEALRLARLARAAGM
                     PEWVKLEITPEPRYLLPDPVETLTAAEALVKDGFTVLPYIGADPILAKRLEEVGCATV
                     MPLGSWIGSNKGIRTRDAVQIIVEQATVPVVVDAGLGAPSHASEAMEMGCGAVLVNTA
                     IAVAADPVGMATAFRLGVEAGRRAFMAGRAPDHEAAEASSPLTGFLDRLVTSQADPGV
                     TEP"
BASE COUNT          553 a         1436 c         1469 g          587 t
ORIGIN      
        1 acgcgatgtt gatcaggtag ctcgagtgcg cgaacgccgg gccgatgccc gacgcatcgc
       61 gccgcgcgcg gaactctcgc gccgcgtcgt ccgagtggtt cggcggcgcc cacgcacggg
      121 gattcgagac gaacatctgg aaggcatcgc acccggccgc tttcgcgtac tcgatcgcgc
      181 cccacagccg tccgggggtc gggacgtgcg cgccgaaccg catcagcgcg tcaaccgccg
      241 atgtagatcc tgacccggtc tccctggcgg accgtcgtgc cggcgctcgg ccgctgcccc
      301 acgacggtgt tcccgtcgga cccgggcacc tcgagcggcg cgactacgag cccgagctcc
      361 tcgagctgct cgacggcggc ctcgcgcgag tcaccgacga cgtcgggcat cgggaagcgg
      421 gccggaccct gcgagacgac gatcgtcacc gaagagtgcg ccggcacggt gttccccgtc
      481 ggcgggtcct gagagatcac gaggcccttc ctgacgtccg acgagttctc gcgccggacc
      541 tcgaccacga gtccctgctc ctcgaggacc gacaccgcgt cgccctcggg gagcccgacg
      601 acgttctgca cctcgaccgg cggctcaccg cggctgaccg tgaccgtgat ctcctcgccg
      661 acctccgggc gcgagcccgg gttgaggctc tgctcgatca cgcggccctc cgggacgtcg
      721 tcgctgtagg cgcgcgcgac gctcaggcgg agctcggccc gccggagcct cctccgggcg
      781 cgatcgatcg gatcgcccac gacgttcggg acctcggtga gctccggtcc cctggagacg
      841 acgaccgaca gcggcgtgcc ttcctccaca cgttcgccgg cgggtggtct ctggctgatg
      901 acgacgttcg cggggacgct cgaggagaac cgctccctcg agatcgcgaa ctccagtccg
      961 cgcgcttcgg cgaggcgctg cgcctcggcc accgatcttc cctcgatgtc gggcgtcgcc
     1021 acgatcacgg ggcggaggtt catgaccgcg gcccacgccg cgacgccgag cgtcgcgagc
     1081 agcgcgagga tcccgacgac catcgcgacg cggcggcgac ggcgggcgcg aggctcgacg
     1141 cgcgggatcg tcaccgtcgc cgcgcgatcg ggtccgcgct cctcgcgcgg cgcggcgttc
     1201 gcggcgagtt ctgcgagcgg ctcggccggc ggcaggtccg gtgccacgcg agcaacgtcc
     1261 agtcgcatcg tgtacgcgtc gcgtgttcgt ccctccgggt cgcgttccgt cgcggagaga
     1321 acgacctgat cgagcgacgg cgggaccatc gggttcgacc gcgacggggg cgggacgcgg
     1381 tcccgcaggt gcttgtacgc gatcgcgacc gacgtctcgc cgctgaacgg cacctggccg
     1441 gtcagcagct cgtacaggac gatccccgtc gcgtagaggt cggtccgcgg gtcggcgggg
     1501 aagccctcga tctgctcggg cgcgagatac tgcacggtgc cggtgacggt ccccggcgcc
     1561 tggctgatgc gcgcgtcggc gagcgctcgc gcgagcccga agtcggtgac cttcacggcg
     1621 ccgtcggacg cccggaccat cacgttctcc ggcttgatgt cgcgggggac gaggccctgg
     1681 cggtgcgcgt gctcgagggc cgcgaggacc tgcgagacga cgtcgacggc ctgcgacggg
     1741 gcgagggccc cgtcgcgcag cagcagcgtg cgcaggttgg gcccgcggat gtactccatc
     1801 accatgaagt aggtcccggc ggtctcgccc cagtcgtgga tcgcgacgat gtttgggtgc
     1861 gagagcatcg cggcggcgcg ggcctcgcgt cggaagcgct cgatgaaccc tgggtcgtcg
     1921 gcgaagctcc tgtgcaggac cttgatcgcg acctcgcggt cgagcaccgg atcgtgggca
     1981 cggtagacct cgcccatccc gccggaagcg acgcgggcgg cgatgacgta acggccgtcg
     2041 acggtggtgt cgagcatgat tggggggagc cggcccacgc cattgtaggt gccgctcgag
     2101 cacgaggccc gtagccgagt aacaatgctg cccgtgagcg acgcccggcc ggatgaggac
     2161 taccaggacg cgttcgctcg catcgagcgt cgggtcgagg ccggcgacgc cgacctgtcc
     2221 gagctcggtt tctggcggct cgtccgcaag gtgaaggtcg agccggcgct cgcggcgcac
     2281 tgggcggacg actgcggccg catcgaccgc atggcgttcg agcggcgggt ccgcccccgg
     2341 ttcccggttt ggctcggcaa cgcgctgctc ctcgtcgggt cgatcgtgct cgtggcgctc
     2401 gtccccgcgg cgctcgcgat cgcgcgagga gagtggctct tcgaacggcc caacccgctc
     2461 gtcgccggca tcctggccgt cgtggcggcc ggcggcctct cggtcacgac ccacgacctc
     2521 gcgcactggg tcgtcgggcg cctcgccggc atccggttca tcgcgtactt cctcgatggg
     2581 ccgacccgga tccagcccgg gctcaagacc gactacgaga cctacctcaa ggcctctccg
     2641 gggagccgcg cggcgatgca tgccgcgggg gcgatcgcct cgaagatcgc gccgttcgcg
     2701 gtcttcgtcg cggtgtacct gccgcaccgc gcgtccggct acggcctgct gcccgcctgg
     2761 tcggtgtgga cgatcctcgc gatcggcgtc gtgcagctcc tgaccgacat cctttggtcg
     2821 acgaagacct ccgactggaa gaaggtgcgc cgcgaggctc gcctcgggcg ggcgcagcgg
     2881 caccgccggt agagacgccg gtgctatcat cgacggtaca agcgaatacc gccggggagc
     2941 tcgggcaacc gggctgagag gccggctgga agcagcgccg gcgacccgcg aacctgaacc
     3001 gggtaatgcc ggcgcaggga gcgcagatcg agggccgccg ccgagcggcc ctttccgttt
     3061 cgaggagcct gatgacgacg agagaagttg cgaccgcacg cgacccgctc gtgatcgcgg
     3121 gccggacgtt cacgtcccgc ttgatcgtcg ggaccggaaa gttcgggtcg tacgacgcca
     3181 tgcgcgacgc gctgacggcg tcgggcaccg agatggtgac ggtcgcgctg aggcgcgtgg
     3241 acctcgactc gaccggcggc cccgacatcc tgcagttcat cgatcccgac cggtacctgc
     3301 tgctcccgaa cacgtccggc gcggtggact cggacgaggc gctgcgtctc gcgcggctcg
     3361 ctcgcgcggc gggcatgccg gagtgggtca agctcgagat cacgcccgaa ccgaggtacc
     3421 tgctcccgga cccggtcgag acgctcacgg ccgcggaggc gctcgtgaag gacgggttca
     3481 cggtcctccc gtacatcggc gcggacccga tcctcgccaa gcggctggag gaggtcgggt
     3541 gcgccaccgt catgcccttg ggatcgtgga tcgggtcgaa caagggcatc cggacgcggg
     3601 acgcggtgca gatcatcgtc gagcaggcga cggtccccgt cgtcgtcgac gcgggcctcg
     3661 gcgcgccgtc gcatgcgtcg gaggcgatgg agatggggtg cggcgccgtg ctcgtcaaca
     3721 ccgcgatcgc ggtagccgcc gatccggtcg gcatggcgac ggcgttccgg ctcggcgtcg
     3781 aggcgggccg ccgcgccttc atggcgggcc gcgcccccga ccacgaggcc gcggaggcat
     3841 cgagccccct caccggtttc ctcgaccgcc tcgtcacctc gcaggcggat ccgggagtga
     3901 ccgagccgtg aagctcgagc ggctcgaaga tctagccgcg ccgatctcgg agcgcgacgg
     3961 tttccttccg ccgtacccgc acgcgacgtt cgcggaggag ctcgcgcggc tgcccttcga
     4021 tcggctcggg gacgtcgcgc gccac
//
LOCUS       DASZOB010000094         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132200, whole genome shotgun sequence.
ACCESSION   DASZOB010000094 DASZOB010000000
VERSION     DASZOB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132200"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="topA"
                     /locus_tag="VG709_01495"
     CDS             complement(<1..1172)
                     /gene="topA"
                     /locus_tag="VG709_01495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWC31785.1"
                     /translation="MAKNLVIVESPTKAKTLERYLGREYAVKASYGHVRDLPKSRMAV
                     DTDRNFDPEYVVPDEKKKVVTALKKAHKSAERLWLATDFDREGEAIAYHVAEVLGVDP
                     GEARRVTFTEITQDAVRDAFEHPRTVDVRLVEAQQARRILDRLVGYNISPLLWKKIRP
                     GLSAGRVQSPALRLIVERERAIQSFRATEYWSTHARVSPDGDELAFVVDLRQIGDEKL
                     ATDPDKKRGLVLGDEATAREHVGNLEGAAYRVEDVTRSERKRNPSPPFTTSTLQQEAA
                     RKLGFSSRKTMRVAQQLYEGVHLPDEGQVGLITYMRTDSVNIAEQALSEIAGVVQRQY
                     GNEYGLEKPRRFKKKQRGAQEAHEAIRPTSATRLPDAIATHLDRDQARLYRLIWQRA"
     gene            complement(1181..1696)
                     /locus_tag="VG709_01500"
     CDS             complement(1181..1696)
                     /locus_tag="VG709_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWC31786.1"
                     /translation="MREPEPREGERVSLTYVGFWPRLGAMLFDVFVVGRLIGAGFGRL
                     WSLYVPDPRALGAIRVSQLLVAALFWAYLVISTWLWGATLGKRVFRLRVVSLDYGRPD
                     LQTALFREVVGRIIVAATLFIGYLQVGSDPRKQGWHDKIADTFVVRRFTLLEVPDPWA
                     ERERAASAQAQ"
     gene            complement(1696..>2178)
                     /locus_tag="VG709_01505"
     CDS             complement(1696..>2178)
                     /locus_tag="VG709_01505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWC31787.1"
                     /translation="GVIMVLLNLESAAGKLGIEPIVIKAGRFKDIGSPFRDISRRERA
                     ILQGLIDEAYERFLSVVEQGRDVPEDELREVADGRLLSGAQAEDAGLVDELGDFDRAV
                     EEARELADIEQARVVEYEAPFSLFGALGDGFPSIGDPVEQLEGSIGVTGPVLKYLYVS
                     "
BASE COUNT          329 a          761 c          731 g          357 t
ORIGIN      
        1 gcgcgctgcc agatcagccg gtacagccgc gcctggtcgc gatcgaggtg cgtggcgatc
       61 gcgtcgggca accgcgtcgc cgaggtcggg cggatcgcct cgtgcgcctc ttgggcgccc
      121 cgctgcttct tcttgaagcg acgcggcttc tcgagcccgt attcgttgcc gtactggcgt
      181 tgcacgacac ctgcgatctc cgagagcgcc tgctcggcga tgttcaccga gtcggtccgc
      241 atgtaggtga tcagcccgac ctggccctcg tcggggaggt ggacgccctc atacagctgc
      301 tgcgcgaccc gcatcgtctt gcgcgacgag aacccgagct tccgcgccgc ctcctgctgc
      361 agcgtcgagg tcgtgaacgg cggcgagggg ttccgcttgc gctccgaccg agttacgtcc
      421 tcgaccctgt acgcggcccc ctcgaggttc ccaacgtgct cgcgcgcggt ggcctcgtcg
      481 ccgagcacga gaccccgctt cttgtccggg tcggtcgcga gcttctcgtc gccgatctga
      541 cggaggtcta cgacgaacgc gagctcgtcc ccgtcggggc tgacgcgcgc gtgggtgctc
      601 cagtactcgg tcgcgcggaa cgactggatc gcgcgctcgc gctcgacgat gagacggaga
      661 gccggcgact ggacgcgtcc ggccgacagc cccggccgga tcttcttcca caggagcggc
      721 gagatgttgt agccgacgag ccggtccagg atgcggcgcg cctgctgcgc ctccacgagc
      781 cgcacgtcga cggttcgcgg atgctcgaag gcatcgcgga cggcgtcctg tgtgatctcg
      841 gtgaacgtga cacgcctcgc ctccccggga tcgacgccga gcacctctgc gacgtggtac
      901 gcgatcgcct cgccctcgcg gtcgaagtcg gtcgcgagcc agagacgctc cgcgctcttg
      961 tgcgccttct tcagcgcggt cacgaccttc ttcttctcgt cggggacgac gtactcgggg
     1021 tcgaagttcc ggtcggtgtc gaccgccatc cggctcttcg gaaggtcgcg gacgtgaccg
     1081 tacgacgcct tcacggcgta ctcgcgcccg aggtagcgct cgagcgtctt cgccttggtc
     1141 ggcgactcga cgatcaccag gttcttcgcc atctacttcc tcactgtgct tgggcggacg
     1201 cggcgcgttc gcgttcggcc caggggtcgg ggacttccag cagggtaaac cgccgcacga
     1261 cgaaggtatc ggcgatcttg tcgtgccacc cctgcttgcg ggggtcggat ccgacctgaa
     1321 gatagccgat gaagagcgtc gcggcgacga tgatccttcc gacgacctcg cggaacagcg
     1381 cggtctgcaa gtcggggcgt ccgtagtcga gcgagacgac gcggagccgg aacacccgct
     1441 ttcccagcgt cgcgccccag agccacgtcg agatcacgag gtaggcccag aagagcgcgg
     1501 cgacgaggag ctgcgatacg cggatcgcgc cgagcgcgcg ggggtccggc acgtagagcg
     1561 accaaaggcg cccgaacccg gcgccgataa gccggccgac gacgaacacg tcgaagagca
     1621 tcgcgcccag cctcggccag aaccccacgt aggtaagcga tacacgctcg ccttcgcgcg
     1681 gctcgggctc gcgcatcacg acacgtagag gtacttgagg acgggtccgg tgacgccgat
     1741 cgagccctcg agctgctcga ccgggtcccc tatagagggg aatccgtcac ccaacgcgcc
     1801 gaacagcgag aacggcgcct cgtactcgac gacgcgggcc tgttcgatgt cggccagctc
     1861 cctcgcctct tcgacggcgc ggtcgaaatc gccgagctcg tcgacgaggc cggcatcctc
     1921 ggcctgcgct ccagagagca ggcggccgtc ggcgacctca cggagctcgt cctccggcac
     1981 gtcgcgcccc tgctcgacga cgctcaagaa gcgctcgtag gcctcgtcga tcaggccctg
     2041 gaggatcgcg cgctcccgcc ggctgatgtc gcggaacggc gagccgatgt ccttgaagcg
     2101 cccggccttg atgacgatcg gctcgatgcc gagcttgccg gccgcgctct cgaggttgag
     2161 cagcaccatg atcacgcc
//
LOCUS       DASZOB010000095         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_2088,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000095 DASZOB010000000
VERSION     DASZOB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VG709_01510"
     CDS             complement(<1..1872)
                     /locus_tag="VG709_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC31788.1"
                     /translation="MPASTATPVGRAREIAMLRAFLEAPPASGALLIEGEPGIGKTTL
                     LSLGVAEARDAGYRVLRAAPAATETKLSFSALGDLLENTGPGLLEALPPPQRRALGGA
                     LLRVDVEGRPPDRRTVSVAVLEVIRSMAAEGPVLIAIDDIQWLDRPSARVLEYTARRL
                     RTERVAFLLSRRTGDSTDPSGLLGALGPDRLRHLELTGLSVGALGRVLANQQGPVLNR
                     PLLLKVRRTSGGNPLFAQEIVRALRRDGQPTPSHSSLPIPDSLRAAVRDRVGELPAGA
                     REAVLVVAALASPTRNVVRDALGPRAESSVAAAVDADVVTLENGRVLLAHPLIGSVLY
                     DEATESERRSVHARLAEAVADPEEKARHLALAATGPDAAVAGALRRAAGIARSRGAPE
                     AAAELSDRAVELTPPEETDVRNELTVEAADHHFASGDADRAREALERLAAALPPGPER
                     ARILWRLARIRFITESLGQAGAILRQSLDEAGDDVALRAHVEQALAFTEMVGGNLPLA
                     QQHARSAVALAARADDAAVTALALSRLATTEFILGMGLDRPSFERSMRLEGALDPDIP
                     IEWRPAYNFAGVLMYADDFSGAREILLSLLRGARDRGDEGALPSILFLMSELECRAGD
                     "
     gene            complement(1961..>2250)
                     /locus_tag="VG709_01515"
     CDS             complement(1961..>2250)
                     /locus_tag="VG709_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWC31789.1"
                     /translation="IMEGGPGTDDFNGGPGADTLRGLGGADFLNGGDDADCLVGGTAR
                     DDYRGGLGNDTLLARDSTRDTGSGGPDPDRGRFDAVDNVASVANRSFAGGC"
BASE COUNT          326 a          790 c          826 g          308 t
ORIGIN      
        1 gtcgccggcc cggcactcca gctcgctcat caagaagagg atcgagggaa gcgcgccttc
       61 gtcgccccgg tcgcgggctc cgcggagcag cgagagcagg atctcgcgcg cgccggagaa
      121 gtcgtcggcg tacatcagga cgcccgcgaa gttgtaggcg gggcgccact cgatcgggat
      181 gtcggggtcg agcgctccct cgagccgcat cgagcgttcg aacgacgggc gatcgagccc
      241 catcccgagg atgaactcgg tggtcgcgag acgggagaga gccagcgcgg tcaccgccgc
      301 gtcgtccgcg cgcgccgcga gcgccacggc cgatcgcgcg tgctgctgag cgagcggcag
      361 gttcccgccg accatctcgg tgaacgccaa ggcctgctcg acatgcgcgc ggagggcgac
      421 gtcgtccccg gcctcgtcga gcgactgacg gaggatcgcg ccggcctgcc cgaggctctc
      481 ggtgatgaac cggatgcggg cgagccgcca caggatcctc gctcgctccg gccccggcgg
      541 gagcgccgcg gcgagacgct caagggcctc ccgggctcgg tccgcgtcgc cggaggcgaa
      601 gtggtgatcc gcggcttcga ccgtgagctc gttccgaacg tcggtctcct ccggcggcgt
      661 cagctcgacc gctcggtcgc tgagctcggc agccgcctcg ggtgcgccgc gggagcgcgc
      721 gatacctgcg gcacgccgaa gtgcgccggc caccgcggcg tcgggccctg tcgccgcgag
      781 cgcgaggtgt cgcgccttct cctcggggtc ggcgaccgcc tccgcgagac gagcgtggac
      841 cgacctccgc tcgctctccg tcgcttcgtc gtagagcacg gagccgatga gcggatgggc
      901 cagcaggaca cgaccgttct caagcgtgac gacgtcggcg tccaccgctg cggccaccga
      961 cgactccgct cgaggtccca aagcgtccct tacgacgttc cgtgtggggg acgcgagggc
     1021 agcgacgacg agcacggctt cgcgcgcacc ggccgggagc tcgccgacac ggtcccgcac
     1081 ggcggcgcgg agggagtctg ggatcggaag cgaggagtgg gacggcgtcg gctgcccgtc
     1141 gcggcgcaac gcacggacga tctcttgggc gaacaacggg ttcccgccgg acgtgcggcg
     1201 aaccttgagc agcagggggc ggttcaggac gggtccctgc tggtttgcga gcacgcgtcc
     1261 gagcgcgccg acggacagtc ccgtcagctc gaggtgccgg agccggtcgg ggccgagcgc
     1321 tcccaggagt cccgatggat ccgtggagtc gccggtacgc ctggagagca ggaacgcgac
     1381 gcgctcagtg cggagtctcc gagccgtgta ctcgaggacg cgtgccgagg gccggtccag
     1441 ccactggatg tcgtcgatcg cgatgagtac ggggccttca gccgccatcg agcggatcac
     1501 ctcgaggacg gcgaccgaaa cggttcgccg gtccggcggc cgtccctcca cgtcgacacg
     1561 tagcagcgcc cctccgagcg cgcgacgttg cggtggaggg agcgcctcga ggagaccagg
     1621 tccggtgttc tcgaggaggt cgccgagcgc cgagaacgac agcttcgttt ccgttgccgc
     1681 aggtgcggcc cggaggaccc ggtagccggc atcgcgcgcc tcggcgacac cgagcgacag
     1741 caacgtcgtc ttgccgatgc ccggttcgcc ctcgatcagg agcgcaccac tggcgggggg
     1801 tgcctccaag aacgcccgaa gcatcgcgat ctcgcgcgct cggccgaccg gggtcgccgt
     1861 ggaggcgggc atcgcggacg gagtctacgt ccaagcggat ggccgcgtcg ccgccgttac
     1921 cgagccgcgt cgaagtggct gaagcgcccg ctgatcccgc tcagcagccc ccggcgaagc
     1981 tcctgttcgc gaccgacgcg acgttgtcga cggcgtcgaa gcgcccacgg tcgggatcgg
     2041 gcccgcctga gcccgtatcc ctagttgaat ctcgagcgag gagcgtgtcg ttcccgagcc
     2101 cgcccctgta gtcgtccctg gcggtgccgc cgacaaggca gtcggcgtcg tcgccaccgt
     2161 tcaggaagtc cgcgccgccc agcccgcgta aggtatcggc gcctgggccg ccgttgaagt
     2221 catcggttcc cggcccgccc tccatgatgt
//
LOCUS       DASZOB010000096         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_153422, whole genome shotgun sequence.
ACCESSION   DASZOB010000096 DASZOB010000000
VERSION     DASZOB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153422"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VG709_01520"
     CDS             complement(<1..1488)
                     /locus_tag="VG709_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWC31790.1"
                     /translation="MTIVEGIAAWAHALKADDVPGDVAALCRTQQRAVLGGVAGSSRN
                     AAARRVISALAEDADDGPAPLLTTDRNVAIDDALYAAAALSMALDFDDYVCFGHSGHS
                     SVLVPLFLAYERDRDAPGRLAAQAVANEVSARLGGACLIGPLNGQMWSFIHAAGAALA
                     AGKMLGLDERALAQALALALYQAPRPVVPGFMAPDSKLLTAAEPIAQGVRAARLAAHG
                     VTGPLDVLDRSAGFFSAFSYAPIRGLLGGLGKGWATKTLCVKPYPGCAYIDSTIDALG
                     QLGPVDPTEVERVEVQAGLLTCGMDALSQRYTRAGALTPVTINFSLGLNVAVYLLAGR
                     LGPRELDDAWLRDHSSELRGLAERVHLEHDWELTRSAMPSFARLLPPGLVRREVGGAA
                     LARGLVRARRDHPRVPIGLKDARALIATFGTDSVPNLAKSYWDPGALADFRMELPARV
                     TITTKSGRRKAAEVRVPRGAAGHADGPAPVASAKLLEVGPWLWGEE"
     gene            1617..2450
                     /locus_tag="VG709_01525"
     CDS             1617..2450
                     /locus_tag="VG709_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWC31791.1"
                     /translation="MTTKRSSRPSPQATGAISLDGDRALARGDAVLPRLTGLLRGTPD
                     RTRTAIGSWSVADVAAHLALLFELYPAIVRGQPSPVTDLDDLPALSASFLEAYPERDV
                     DVLADKVESAWRGYATAVREVGSEQMMTWHGGIQMRVATLTGILLGEMLVHGYDVARA
                     IGRPWEIQRQDAVLTLKAIAELLPHYLTEEGRRARASFRLVVRGGDPLTMRFHSGTLE
                     VDEHDEDPVDCKMSIDPVAFLLVGYGRTGLWRAVLKGQMFAYGRKPWLSLRFQRYLRN
                     P"
     gene            2486..>2989
                     /locus_tag="VG709_01530"
     CDS             2486..>2989
                     /locus_tag="VG709_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018808863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWC31792.1"
                     /translation="MVGGIHYEDPFATPVEKREPWRRLRGRLASPVTLWTSGSREART
                     GLTISSVVVADGQPPSVLGLMKDTSDLFASIQESGRFVVHVVERDDRALADRFAGLRP
                     SPGGLFAGEEVEDSEWVPVLTRLVNRAYCRFVSERAAGYQRLVEGTIEQIDLDELESP
                     LVLFRGRY"
BASE COUNT          490 a         1005 c         1049 g          445 t
ORIGIN      
        1 ctcctcgccc cagagccagg gaccgacctc cagcagcttg gcgctcgcta cgggggccgg
       61 tccgtccgcg tgtcccgccg cgccgcgggg gacgcgcacc tcggcggcct tgcggcgacc
      121 ggacttcgtc gtgatcgtca cccgagcggg gagctccatg cggaaatccg ccagcgcgcc
      181 cgggtcccag taggactttg ccaagttcgg tacggaatcc gtaccgaaag tggcaatcag
      241 ggcgcgcgcg tccttcaacc cgatggggac acggggatgg tcacgccgcg cgcggacgag
      301 cccacgcgcc agcgcggccc cgccgacctc gcgccgcacg aggcccggag gcaacaggcg
      361 cgcgaacgac ggcattgcgg atctcgtgag ctcccagtca tgctcgagat gcacgcgctc
      421 ggcgagcccg cgcaactcgg acgagtggtc gcgcagccag gcgtcgtcca actctcgggg
      481 gccgagcctg ccggccagca agtagaccgc gacgttgagc ccgagcgaga agttgatcgt
      541 cacgggagtc agggctccgg cgcgcgtgta acgctgcgag agcgcgtcca tcccgcaggt
      601 cagcaatccg gcctgcacct cgacgcgttc cacttccgtg ggatcgaccg gaccgagttg
      661 cccgagcgcg tcgatcgtcg agtcgatata ggcgcatccg ggatacggct tgacgcagag
      721 ggtcttggtc gcccagcctt tcccgagacc gccgagcaag cctcggatcg gagcgtagga
      781 gaaggcggag aagaaccccg cgctccggtc gagaacgtcg agcggcccgg tcacgccgtg
      841 cgcggcaaga cgggcggcgc gcacaccctg cgcaatgggc tcggcggccg tgagcagctt
      901 cgaatcggga gccatgaagc ccggcaccac cggtcgaggc gcctggtaca gcgcgagcgc
      961 gagggcctgc gccagggctc tctcgtcgag gccgagcatc tttcccgccg cgagcgccgc
     1021 cccggcggca tggatgaacg accacatctg tccgttcagc gggccgatca gacaggctcc
     1081 accgaggcgc gccgagacct cgttggccac ggcttgggca gcgagacgac cgggagcgtc
     1141 gcggtctcgt tcgtaggcga ggaagagcgg cacgaggacc gaggagtgac cgctgtgccc
     1201 gaagcagacg tagtcgtcga aatcgagcgc catcgagaga gccgcggcgg cgtagagcgc
     1261 gtcgtcgatc gccacgttgc gatcggtggt gagaagcggc gccggaccgt cgtcggcgtc
     1321 ttcggcgagc gcggagatca cgcgccgggc cgcggcgttg cgagacgagc ccgcgacgcc
     1381 gcccaggacg gctcgctgct gcgtccggca gagcgcggcg acgtcgccgg gcacgtcgtc
     1441 ggccttcagc gcgtgcgccc acgcggcgat cccctcgacg atcgtcactc gcaggacctt
     1501 tccacgtcag aacctccggc gatcgtctgg acgatcgctt agacaaagcg gtcgtgtcac
     1561 ctagcgcgga cgaagcgatc gttctggacg accgcgtaga gaatatggtt gaccctatga
     1621 cgacgaaacg ttcgtcccgt ccatcgccgc aggcgaccgg agcgatatct ctcgacggcg
     1681 atcgcgccct cgcgcgcggc gacgccgtac tcccccgtct gaccggtctg ttgcgcggga
     1741 cgccagaccg caccaggacg gcgatcggta gctggagcgt ggccgacgtg gcggctcatc
     1801 tcgctctcct cttcgagctc tacccggcga tcgtgcgagg gcagccgtct cccgtgaccg
     1861 acttggacga cctgccggca ctgtccgctt cgttcctcga ggcgtacccg gagcgagacg
     1921 tggacgtgct cgcggacaag gtcgagtccg cctggcgcgg atatgcaacc gcggtgcgcg
     1981 aggtggggtc cgagcagatg atgacctggc acggaggcat ccagatgcgc gtcgcgacgc
     2041 tcaccgggat cctgctcgga gagatgctcg tgcacggcta cgacgtcgcg cgcgcgatcg
     2101 gtcgcccatg ggagatccag agacaggacg cggtgctgac gctcaaggcg atcgcggagc
     2161 tgctgcccca ctacctcacc gaagagggca ggcgggcgcg cgcgagcttc cgtctcgtcg
     2221 tgcgaggagg cgatccgctg accatgcgct tccattccgg cactttggaa gtcgacgagc
     2281 acgacgaaga ccccgtcgac tgcaagatgt cgatcgaccc ggtcgcattc ctgctcgtgg
     2341 gctacgggag aacgggactg tggcgcgcgg tgctcaaggg acagatgttt gcctacggtc
     2401 gcaagccctg gctttctctg cgcttccagc gttacctgcg caatccgtaa tcggacgcca
     2461 tagacccgcc gcggtaacgt gcggcatggt cggaggcatc cattacgagg acccgtttgc
     2521 gacgccggta gagaaacgcg aaccgtggcg gcgactgcgc ggacggcttg cgtcgcccgt
     2581 cacgttgtgg accagtggga gccgcgaggc gcgcacgggc ctgacgatct cctcggtcgt
     2641 cgtcgccgac ggccaaccgc caagcgtgct gggactcatg aaggacacgt cggacctctt
     2701 cgcctcgatc caggagagcg ggcgcttcgt ggtgcacgta gtcgagcgcg acgaccgagc
     2761 gctcgccgat cgttttgccg gactacggcc cagccccggc gggctcttcg ccggtgaaga
     2821 ggtggaggac tcggagtggg tgccggtcct cacgcggttg gtcaatcgcg cctactgccg
     2881 cttcgtgagc gagcgggccg cgggttatca gcgcctggtc gagggcacga tcgaacagat
     2941 cgacctcgac gagctggaga gccccctcgt gctcttccgg gggcgctac
//
LOCUS       DASZOB010000097         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_183739, whole genome shotgun sequence.
ACCESSION   DASZOB010000097 DASZOB010000000
VERSION     DASZOB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183739"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VG709_01535"
     CDS             complement(<1..204)
                     /locus_tag="VG709_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31793.1"
                     /translation="MVHRTLTTFVTAAVAAVTASPALAQTSPTPPGVQPAWRIVMYVL
                     VGIVLLALLVWGFAALNRRRGAGE"
     gene            306..884
                     /locus_tag="VG709_01540"
     CDS             306..884
                     /locus_tag="VG709_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31794.1"
                     /translation="MPEHTHQHGGLPPHDHDAPGLDHDHDDEVVDRDREVERDTRAET
                     AVTVAPAPGGVAARILLTILGAAGMIIGAFLSWFAGQNVRGVDFEFRVFYSTDVGGEA
                     GFVASAGFILIILGLIALLGLAFRVGWLTSFAGALGLLAMVAFTVTLYRGGEGELGLG
                     DVGIGAWICAIGSLITLIAGWFGGRDRVVAAA"
     gene            929..1468
                     /locus_tag="VG709_01545"
     CDS             929..1468
                     /locus_tag="VG709_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31795.1"
                     /translation="MDTERVEGPAPAPTIARIIMLVLAGALLIFGSFLTWIGLGQLGD
                     VAAQLGQELPSTAGVNLEFSIYYSATQDPTGGGEVNIFSSAGLVTIAAGGLALLGLLI
                     PWLARLGGAIGVIAFVAFVITLYRVEDQPGFSFGIGNVGIGMWMVLAGSILALIGGFL
                     VARRTVVTSTAAAEPPPEP"
     gene            complement(1471..2325)
                     /locus_tag="VG709_01550"
     CDS             complement(1471..2325)
                     /locus_tag="VG709_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWC31796.1"
                     /translation="MSTDALERSVAAIAARIIGQQPGRDEPLPFDSLSHAELVFALEE
                     ELAVRLPDQVSLRTLAEATDAVERARGNGGSGAPLHEGIGHLQWLAAGALRPILGPYY
                     RFSVTGADRIPDRGPVVLVSNHDSLLDIPFLAIAAPRRVWFMAKRELFEKRFGAWFFH
                     VLGAFPVDRGRNDLKALRAALATIEQGRALAMYPEGTRSREFLPFLPGAAWGALATGA
                     PLVPVAVRGTAESMPRGSVFPRRSRVSVAFGEPVEPGLERDPRRRLERAAKLTVELRG
                     AVERLRWP"
     gene            2477..3427
                     /locus_tag="VG709_01555"
     CDS             2477..3427
                     /locus_tag="VG709_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWC31797.1"
                     /translation="MQMYRTMVTARLCDEQQFRLNRQGRAPFVVPVSGHEGCQIATAW
                     PMVRGKDIFVPYYRDMGVCLVAGMTPKDVFLGVFGKADDPSSGGRQMPAHWGSRRLGI
                     ITGSSPISTQVPHASGIAYAMKYRGEDAIVGSWVGDGGTSEGDWHEALNFAGIHKLPI
                     VFVIENNLYAISVPLSKQAGVEKLSVRAEGYGFPGVTVDGNDIFETYEAMKQAVERAR
                     SGEGPTLIETMTYRFHPHTSDDDDRTYRPREEVEEAKRKDPIVAFLGYLREIGLMDDD
                     KVEAIRGEIKEQVDKAVEEAWSAPDPEPESALRHVFAEGS"
     gene            3429..>4302
                     /locus_tag="VG709_01560"
     CDS             3429..>4302
                     /locus_tag="VG709_01560"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWC31798.1"
                     /translation="MAEKNIVNTIHDTLWEEMKSDDRVLVLGEDVGARGGVFRATAGF
                     LDEFGEQRVIDTPLAESSIVGVAIGMALHGLLPVAEIQFADFIHPAFDQIVSEGARIR
                     YRSNGDFGVPMVIRAPWGGGVHGALYHSQSIEGFYGHVPGLKVVTPSTPYDAKGLLRA
                     ALRDPDPVMFLEHKKTYRLIKGDVPDEDYEVPIGKADVKREGDDLTCVAYGLMLHYCM
                     EAANKLQEEDGVSVEVVDVRTIAPLDKETILESVKKTGKAMVVYEDNRTYGVGAEIMA
                     TIAEETMFDLDGPLV"
BASE COUNT          695 a         1472 c         1521 g          614 t
ORIGIN      
        1 ctcccccgcg ccgcgacgcc ggttcagcgc cgcgaatccc catacgagca acgcgagcag
       61 cacgatcccc accagcacgt acatcacgat gcgccaggcc ggctggacac cgggaggcgt
      121 cggactcgtc tgagccagcg caggcgacgc ggtcaccgcc gcgaccgcag cggtcacgaa
      181 cgtcgtgagc gtgcgatgga ccatccgacg agttcgttcc cgcggcaccc gggggtgaaa
      241 ccgatggggt tgacgcggtc gaacggaggg gaacaataca aggaatccga agagggagac
      301 aagcgatgcc tgagcacacg catcagcacg gcgggcttcc gccgcacgac cacgacgccc
      361 ccggcctcga ccacgaccac gacgacgagg tggtcgaccg ggacagggag gtcgagcgcg
      421 acacacgggc ggagaccgcg gtcaccgtgg cccccgctcc aggtggcgtc gcggcgcgga
      481 tcctgctcac gatcctcggc gcagcgggga tgatcatcgg cgcgttcctg tcgtggttcg
      541 ccggtcagaa cgtgcggggg gtcgatttcg agttccgcgt cttctactcg acggacgtcg
      601 gcggggaggc gggcttcgtg gcgtcggccg gcttcatcct catcatcctc ggactcatcg
      661 cgctgctcgg gctggccttc cgcgtcggat ggctcacgag cttcgccggc gcgctcgggc
      721 tgctagccat ggtggcgttc accgtgacgc tgtaccgcgg cggtgaaggg gagctcggac
      781 tgggagacgt cggcatcgga gcgtggatct gcgcgatcgg cagcctcatc acgctgatcg
      841 cgggatggtt cggaggacgc gaccgcgtcg tcgcggcagc atgatggccc ggcgctccgg
      901 cgcgcccacg acctgagagg aggcacccat ggacaccgaa cgcgtggaag gccccgcacc
      961 ggcccccacg atcgcgcgca tcatcatgct ggtcctcgcg ggggcgctgc tgatcttcgg
     1021 ctcgttcctg acgtggatcg ggttggggca gctcggtgat gtcgcagcgc agctcgggca
     1081 ggagctgccg tcgacggcgg gagtcaacct cgagttcagc atctactact cggcgaccca
     1141 ggacccgacc ggcggcggcg aggtgaacat cttctcgtcg gccggcctcg taacgatcgc
     1201 ggccggcggc ctcgcgctcc tcgggctgct gatcccgtgg ctggcacgcc tcggcggagc
     1261 gatcggcgtg atcgccttcg tggcgttcgt catcacgctc taccgcgtgg aggaccagcc
     1321 gggcttcagc ttcggcatcg ggaacgtcgg catcgggatg tggatggtgc tcgccggtag
     1381 catcctcgcg ctgatcggcg ggttcctcgt cgccaggcgc accgtcgtca cgtcgacggc
     1441 ggcggccgag cctccgcccg aaccgtaggg ctagggccag cgcagacgct cgacggcgcc
     1501 gcggagctcg accgtgagct tcgcggcgcg ctcgagccgc ctccgcggat cgcgctcgag
     1561 ccccggttcg accggctcgc cgaacgcgac agagacccgt gagcgtctcg ggaacacgga
     1621 gcccctcggc atcgactccg cggtcccccg cacggcgacc ggcacgagcg gcgcgccggt
     1681 cgccagcgcg ccccacgccg cacccgggag gaacgggaga aactctctgc tccgcgtccc
     1741 ttccgggtac atcgcgagcg cgcggccctg ctcgatcgtc gcgagcgccg cgcgcagcgc
     1801 cttcaggtcg ttgcgtccgc ggtctaccgg gaacgccccc agcacgtgga agaaccacgc
     1861 cccgaaccgc ttctcgaaca gctctcgctt cgccatgaac cacacgcgcc gaggagcggc
     1921 gatcgcgagg aacgggatgt cgagcaggct gtcgtggttc gacaccagga cgaccggccc
     1981 gcggtccggg atgcgatcag cgcccgtaac gctgaaccgg tagtacgggc cgaggatcgg
     2041 ccggagcgcc ccggccgcca gccactgcag gtgtccgatc ccttcatgca gaggggcgcc
     2101 cgaaccgccg ttgccacggg cccgctcgac ggcgtcggtc gcctccgcga gcgtccgcag
     2161 cgacacctgg tcggggagcc gaaccgcgag ctcctcctcg agcgcgaaga cgagctccgc
     2221 gtgcgagagc gagtcgaatg ggagcggctc gtcgcgaccg ggctgttgtc cgatgatgcg
     2281 cgcggcgatc gcagcgacgc ttcgctcgag cgcgtcggtg ctcacgaggc gcgagtctag
     2341 cggcggtccg gctagaatcg ggcggtccgg ccggtacggc caggcggtca cttcacgagg
     2401 agggctagcg gtggcgcagc gagtcaagga agcggccgac cggcactctc cgctcggcct
     2461 caccgaggac gacctcatgc agatgtaccg gaccatggtc acggcccggc tctgcgacga
     2521 gcagcagttc cgcctcaacc gacaggggcg agctccgttc gtcgtcccgg tgtcgggtca
     2581 cgagggatgc cagatcgcga ccgcgtggcc gatggtccgc ggcaaggaca tcttcgtgcc
     2641 gtactaccgt gacatgggcg tctgcctcgt cgccggcatg acgccgaagg acgtcttcct
     2701 cggggtcttc gggaaggcgg acgacccttc ttcgggcggc cgtcagatgc ccgcgcactg
     2761 gggctcgcgg cgcctcggga tcatcacggg ctcctcgccg atctcgacgc aggtccccca
     2821 cgcgtctggg atcgcgtacg cgatgaagta ccgcggcgag gacgcgatcg tcggctcatg
     2881 ggtcggcgac ggcggcacca gcgagggcga ctggcacgag gcgctcaact tcgccggcat
     2941 ccacaagctc ccgatcgtgt tcgtgatcga gaacaacctc tacgcgatct cggtgccgct
     3001 ctcgaagcag gccggcgtgg agaagctctc ggtgcgcgcc gagggctacg gcttccccgg
     3061 cgtgaccgtg gacggcaacg acatcttcga gacctacgag gcgatgaaac aggcggtcga
     3121 gcgcgcgcgc tccggcgagg gcccgacgct catcgagacc atgacgtatc gcttccatcc
     3181 ccacacctcc gacgacgacg accgcaccta ccggccgcgc gaggaggtcg aggaggcgaa
     3241 gcggaaggac ccgatcgtcg cgttcctcgg gtacctccgc gagatcgggc tgatggacga
     3301 cgacaaggtc gaggcgatcc ggggcgagat caaggagcag gtcgacaagg cggtcgagga
     3361 ggcgtggagc gcgccggacc cagaacccga gtcggcgctc cggcacgtgt tcgccgaggg
     3421 gtcctgagat ggccgagaag aacatcgtca acacgatcca cgacacgctg tgggaggaga
     3481 tgaagtccga cgaccgcgtg ctcgtcctcg gcgaggacgt cggcgcgcgc ggcggcgtgt
     3541 tccgggccac ggcgggcttc ctcgacgagt tcggggagca gcgcgtgatc gacacgccgc
     3601 tcgcggagtc ctcgatcgtc ggcgtcgcga tcgggatggc gctgcacggg ctgcttcccg
     3661 tcgcggagat ccagttcgcc gacttcatcc acccggcgtt cgaccagatc gtctccgagg
     3721 gcgcgcgcat ccggtaccgc tcgaacggcg acttcggagt gcccatggtg atccgggcgc
     3781 cgtggggcgg cggcgtccac ggggcgctct accactcgca gtcgatcgaa ggcttctacg
     3841 gccacgtgcc gggcctcaag gtcgtgacgc cttcgacgcc gtacgacgcg aagggactgc
     3901 tgcgcgcggc gctccgcgac cccgaccccg tgatgttcct cgagcacaag aagacgtacc
     3961 ggctgatcaa gggcgacgtc cccgacgagg actacgaggt gccgatcggc aaggccgacg
     4021 tcaagcgcga gggcgacgac ctcacgtgcg tcgcctacgg gctgatgctg cactactgca
     4081 tggaggccgc caacaagctc caggaggagg acggcgtctc ggtcgaggtc gtcgacgtgc
     4141 gcacgatcgc gccgctcgac aaggagacga tcctcgagtc ggtgaagaag accggcaagg
     4201 ccatggtggt ctacgaggac aaccgcacgt acggcgtggg cgccgagatc atggccacca
     4261 tcgccgagga gaccatgttc gacctcgacg gtcccctggt cc
//
LOCUS       DASZOB010000098         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_41586, whole genome shotgun sequence.
ACCESSION   DASZOB010000098 DASZOB010000000
VERSION     DASZOB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41586"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VG709_01565"
     CDS             <1..936
                     /locus_tag="VG709_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWC31799.1"
                     /translation="ARGARAMTALIVAELRQRVRGRRWWIVLVLWTLVLALLLLLVRS
                     AAREAIQFGVTPPGFVGTAMFAALALLVLALASLVVPALSSTAINGERDRGTLAVLQS
                     TPLRSWEIGLAKFVAAWVTAGVFLVATVPLAVWAALEGGVGFARALVVYLVLAILSAV
                     FVAIGLAASALIVRPIISTVTAYAVVFFMTALLPVFFGLALATAPDRVEGGFGKEVGV
                     RWVILAADPFVVLADAAPRAEDPDVPDPLAAIRDVVRSIRDQQYLFPGQRDRVPALWP
                     AGLGIDLALGAGALGLAVVRLKVPARRLPRGQRVA"
     gene            982..1569
                     /locus_tag="VG709_01570"
     CDS             982..1569
                     /locus_tag="VG709_01570"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWC31800.1"
                     /translation="MATVLDAPADTRTRILDAAFRSVERFGLSRFTVDDVAKAAGLSR
                     QTVYRYFDSKDDLVMALVLREEEAFIDGVRAEFETHGDFEAAMTAAIRYCLVSARAHP
                     LLDRLLAAEPDVLLPYLTVRGMPLVTRARTALEDLVGGRIDADRDLVRRAADLAVRAV
                     ISYTLTPTDDRPEDVARGTAKLLTAALRGTRKEKR"
     gene            1566..2705
                     /locus_tag="VG709_01575"
     CDS             1566..2705
                     /locus_tag="VG709_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018813479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWC31801.1"
                     /translation="MSTRQYTLPVDITKWEVPSGSTTIFNWEYDETRDRLLTLYEKGK
                     EKQWNAQTRLDWSVDVDPQAPENGPDYYIPIFGSDMWEALDEAGKGELRQHIGAWMNS
                     QFLHGEQGALICASKIVQTVPDVDSKFYAATQVMDEARHVEMYSKYLREKIELAYPIN
                     ANLQALLSDVISDPRWDMTYLGMQVIIEGLALAAFALIRDFSQEPLAKAINTYVMQDE
                     ARHVAFGRLALKDYYPELTQKERDEREEFVVQACYLMRDRFRAQEVWEHLGFDVKRCL
                     EYVEQSEIMQEFQRMLFSRIVPTVKDIGLWGEKVQRAYEDMGVIQFKDADPDSLSAQD
                     EQLARKLDTGRGLSEEAEAAATLAGVDAKRALEVEQTIRAGGGEG"
BASE COUNT          396 a          955 c          939 g          444 t
ORIGIN      
        1 gcgcgaggag cgcgagcgat gaccgcgctc atcgtcgcgg agctccggca gcgggtccgc
       61 gggcgccgct ggtggatcgt cctcgtcctg tggactctcg tgctcgcgct cctcctgctg
      121 ctggttcgct ccgccgcgcg agaggcgatc cagttcggcg tcacgccgcc ggggttcgtc
      181 gggaccgcga tgttcgccgc gctcgcgctg ctcgtgctcg cgctcgcgtc gctcgtggtg
      241 ccggcgctct cgtcgaccgc gatcaacggc gaacgcgacc gtgggacgct ggcggtgctg
      301 caatcgacgc cgctccgctc ctgggagatc gggctcgcca agttcgtcgc ggcctgggtc
      361 acggccggcg tcttcctggt cgcgaccgtg ccgctcgccg tgtgggcggc gctcgagggc
      421 ggcgtcggct tcgcccgcgc gctcgtcgtc tacctcgtgc tcgcgatcct gtccgcggtg
      481 ttcgtggcga tcgggctcgc agcttcggcg ctcatcgtgc gtccgatcat ctcgaccgtc
      541 accgcctacg ccgtcgtctt cttcatgacg gcgctgctcc cggtgttctt cggcctcgcg
      601 ctcgcgaccg ctcccgacag ggtcgagggg gggttcggga aagaggtcgg ggtgcgctgg
      661 gtgatcttgg ccgccgaccc gttcgtcgtc ctcgcggatg ccgcgccgcg cgccgaggac
      721 cccgacgtcc ccgatccgct cgcggcgatc cgagacgtcg tccgatcgat ccgcgatcag
      781 caatatctct tccccggtca gcgtgaccgg gttccggccc tctggccggc aggcttgggg
      841 atcgatctcg cgctcggagc cggcgcgctc ggcctcgccg tcgtgaggct gaaggtcccg
      901 gcccggcgtc tgccgcgcgg ccagcgcgtc gcatgacccg cgacttgacg acgttacgcg
      961 gcgtgtcata gcgtcacatc gatggccacc gtcctcgacg cccccgcaga cacgcgcacc
     1021 cggatcctcg atgccgcctt ccggtcggtc gagcggttcg gcctctcccg gttcacggtc
     1081 gacgacgtcg cgaaggcggc gggcctgtcg cgtcagaccg tctaccggta tttcgactcg
     1141 aaggacgacc tcgtgatggc gctcgtgctg cgcgaggagg aggccttcat cgacggggtc
     1201 cgcgcggagt tcgagacgca cggcgacttc gaggccgcga tgaccgccgc gatccgctat
     1261 tgcctggtgt cggcgcgcgc gcacccgctc ctcgatcgcc tgctcgcagc cgaaccggac
     1321 gtcctgctcc cgtatctcac ggtccgcggc atgccgctcg tgacgcgcgc ccgcacggcg
     1381 ctggaggatc tcgtcggtgg acgcatcgac gccgaccgcg acctcgtccg gcgcgccgcc
     1441 gatctcgccg tccgcgccgt gatctcgtac acgctcacgc cgaccgacga tcgccccgag
     1501 gacgtcgccc gcggtacggc gaagctcttg accgccgcgc tccgcggcac gcggaaggag
     1561 aagcgatgag cacgcgccag tacacgctgc cggtcgacat cacgaagtgg gaggtgccga
     1621 gcgggtcgac cactatcttc aactgggaat acgacgagac gcgcgaccgt ctgctcacgc
     1681 tctacgagaa gggcaaggag aagcagtgga acgcgcagac ccgactcgac tggtcggtcg
     1741 acgtcgaccc acaggctccc gagaacggcc ccgactacta cattccgatc ttcggctccg
     1801 acatgtggga ggcgctcgac gaggccggga agggcgagct ccgccagcac atcggcgcgt
     1861 ggatgaactc gcagttcctc cacggcgagc agggggcact gatctgcgcc tccaagatcg
     1921 tccagacggt tccggacgtc gactccaagt tctacgcggc gacgcaggtg atggacgagg
     1981 ctcgccacgt cgagatgtac tcgaagtacc tgcgcgagaa gatcgagctc gcctacccga
     2041 tcaacgccaa cctccaggcg ctcctcagcg acgtcatctc agacccgcgg tgggacatga
     2101 cctatctcgg gatgcaggtg atcatcgagg ggctcgcgct cgccgcgttc gccctgatcc
     2161 gcgacttctc gcaagagccg ctcgccaagg cgatcaacac gtacgtcatg caggacgagg
     2221 cgcgccacgt ggcgttcggc cgtctcgcgc tcaaggacta ctaccccgag ctcacgcaga
     2281 aggagcgcga cgagcgagag gagttcgtcg tccaggcttg ctacctcatg cgcgaccgtt
     2341 tccgggcaca ggaggtgtgg gagcacctcg gcttcgacgt gaagcgctgt ctcgagtacg
     2401 tcgagcagtc cgagatcatg caggagttcc agcggatgct gttctcgcgg atcgtgccga
     2461 cggtgaaaga catcgggctc tggggtgaga aggtccagcg cgcctacgag gacatgggcg
     2521 tgatccagtt caaggacgcc gaccccgact cgctctcggc gcaggacgag cagctcgccc
     2581 ggaagctgga caccgggcgc ggcctctcgg aggaggcgga ggccgccgcg acgctggcgg
     2641 gcgtcgacgc gaagcgagcg ctcgaggtcg agcagacgat ccgtgccggc ggcggagagg
     2701 ggtagcgagc cggacgcgct ccggccgctg ccgt
//
LOCUS       DASZOB010000099         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132270, whole genome shotgun sequence.
ACCESSION   DASZOB010000099 DASZOB010000000
VERSION     DASZOB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132270"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VG709_01580"
     CDS             <1..447
                     /locus_tag="VG709_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31802.1"
                     /translation="VFHGCAIVSVKKAFPGHAKKVMHAIWGLGMLSLTKSVVVVDEFV
                     DVHDYEQVFFHVCANVDPGRDVILSEGPLDQLDHAAAMYCYGGKLGIDATHKWTTEGA
                     REWPERIEMSAEVRDLVDRRWSEYGIDAVAEQNGRISRGLRQKLRL"
     gene            515..1618
                     /locus_tag="VG709_01585"
     CDS             515..1618
                     /locus_tag="VG709_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31803.1"
                     /translation="MADELERPGDDVERHEGSPHLPPPSIWPFGFALGVTLVLVGLIV
                     SWFIVAVGAVISIVFAFLWIREATREVRGRPAPEPARREAVADADEEAEEAPRYSRSV
                     FLEGATLGIGAAIGGIVTLPALGFAVLPAFVGQEYDEVDLGPLDNFPEGQWIVTKFRS
                     VADEPEDVSRRTAYVRNNGTSGGEPSFTIISNRCVPLGCPVQPQGLTDDEPREVETES
                     GRVELFGTSPSGFVCPCHGGSYTIEGNRQAGPPVRALDRYSYSIRDGRLVIGQPYSVS
                     EVVGEGADARIEAYKIVDPGVHVDGPEQFFYPYVPCWRAHDRSAGSRSKSSSSCRSTG
                     SRSARASSARFATSSSAASRPRRTGSTRSARPP"
     gene            1615..>2101
                     /locus_tag="VG709_01590"
     CDS             1615..>2101
                     /locus_tag="VG709_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWC31804.1"
                     /translation="MTAFLVQLVTGVILAMYYVPDPDKAYQSIQTITNDLTLGWLVRG
                     MHRWGASVFIILLFLHMGRVFLFGAYKYPRELNWVIGVVLLVLTLAMGLTGYLLPFDQ
                     RGFWATVVAININSTAPIAGPFLADFLRGGAEIGQDTLSRFYSLHMLVIPGAMAALIA
                     LH"
BASE COUNT          322 a          730 c          698 g          351 t
ORIGIN      
        1 gtcttccacg gctgcgcgat cgtctccgtg aagaaggcgt tcccgggcca cgcgaagaag
       61 gtcatgcacg cgatctgggg gctcgggatg ctcagcctca cgaagtccgt cgtcgtggtg
      121 gacgagttcg tcgacgtgca cgactacgag caggtgttct tccacgtctg cgcgaacgtc
      181 gaccccgggc gcgacgtgat cctctccgag ggcccgctcg accagctcga ccacgcggcc
      241 gcgatgtact gctacggcgg caagctcggg atcgacgcga cgcacaagtg gacgaccgag
      301 ggagcacgcg agtggcccga gcggatcgag atgagcgcgg aagttcgtga cttggtcgac
      361 cgccggtggt cggagtacgg catcgacgcg gtcgccgagc aaaacggccg gatttccagg
      421 ggattgcgcc aaaagctacg tctttgacac gagattcgaa aagagagaga atcgcaccgc
      481 ttgtaacagc gcaagcgtct agggagggag ctgtgtggcc gacgagctag agcggcctgg
      541 tgacgacgtc gagcgtcacg aaggctcccc gcatctcccg ccgccctcca tctggccgtt
      601 cgggttcgcg ctcggcgtca ccctcgtcct cgtaggcctc atcgtcagct ggttcatcgt
      661 cgccgtcggc gccgtcatca gcatcgtctt cgcgttcctc tggatccgcg aggcgacccg
      721 cgaggtccgc ggcaggccgg cgcccgagcc cgcgcgccgg gaagccgtcg cagacgcgga
      781 cgaggaagcg gaagaggcgc cgcggtactc ccgctcggtg ttcctcgagg gcgccactct
      841 cgggatcggc gccgcgatcg gcggcatcgt cacgctcccc gccctcggct tcgcggtctt
      901 gcccgcgttc gtcggccagg agtacgacga ggtcgacctc ggcccgctcg acaacttccc
      961 ggagggccag tggatcgtca cgaagttccg gtccgtcgcg gacgagcccg aggacgtctc
     1021 gaggcgcacc gcgtacgtcc ggaacaacgg cacgagcggc ggcgagccga gcttcacgat
     1081 catctcgaac cgctgcgtcc ccctcggttg cccggtgcag ccgcagggcc tcacggacga
     1141 cgaaccgcgc gaggtcgaga ccgagtcggg acgcgtcgag ctcttcggga cgagcccctc
     1201 ggggttcgtg tgcccgtgcc acggcgggtc gtacacgatc gaggggaacc ggcaggcggg
     1261 gccgcccgtc cgcgcgctcg accgctacag ctattcgatc agggacggca ggctcgtcat
     1321 cgggcagccg tacagcgtct ccgaggtcgt cggcgagggc gcggatgcga ggatcgaggc
     1381 gtacaagatc gtcgacccgg gcgtccacgt cgacggtcca gagcagttct tctacccgta
     1441 cgtgccctgc tggcgcgcac acgatcgaag cgccggcagc aggtcgaaga gctcgtcctc
     1501 ctgccggtcg actggctcga ggagcgctcg ggcctcgtcg gcgcgcttcg ctacttcctc
     1561 ttccgccgcg tcccggccga gacgaactgg ttccacacgc tcggctcggc caccctgacg
     1621 gccttcctcg tccagctcgt gaccggcgtc atcctcgcca tgtactacgt gccggatccc
     1681 gacaaggcct accagtcgat ccagacgatc acgaacgatc tgacgctcgg ctggctcgtg
     1741 cgcggaatgc accgctgggg cgcgagcgtc ttcatcatcc tcctcttcct gcacatgggg
     1801 cgcgtctttc tcttcggcgc ctacaagtac ccgcgggagc tcaactgggt gatcggcgtc
     1861 gtcctgctcg tgctcacgct cgcgatgggg ctcacgggct acctgctccc gttcgaccag
     1921 cgcggcttct gggcgacggt cgtcgcgatc aacatcaact cgacggcgcc gatcgccggg
     1981 ccgttcctcg cggacttcct ccgcggtggt gccgagatcg gccaggacac gctctcgcgg
     2041 ttctactcgc tccacatgct cgtgatcccc ggtgcgatgg cggcgctgat cgccctccac
     2101 c
//
LOCUS       DASZOB010000100         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_163583, whole genome shotgun sequence.
ACCESSION   DASZOB010000100 DASZOB010000000
VERSION     DASZOB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163583"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VG709_01595"
     CDS             complement(<1..426)
                     /locus_tag="VG709_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWC31805.1"
                     /translation="MGRYGEVVRYFLRLGTIGFGGPNAHIAAMHDDLVRRRRWVDERH
                     FLEVVGVTNLIPGPNSSEVAIHLGYLRAGPLGGILAGVAFFLPAFVIVTALAWVYFEV
                     GGFRFRGDLLAGVQAVGLAAIVATLWRLRGGVVGGWRKPA"
     gene            557..1672
                     /locus_tag="VG709_01600"
     CDS             557..1672
                     /locus_tag="VG709_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWC31806.1"
                     /translation="MTKRLVSVIAATSVVAWIVLSPGAAGAARPKCKGKRATIVGTNR
                     SERIVGTPRRDVIVAKGGRDVILARNRADLICAGPGNDFVRAGGGNDAVYGQLGADIL
                     AGDAGADVIAGAAGAELLYGGDGNDTMRAGTGASSVMYGNRGNDRMFGGDGFDTLLGL
                     EGNDRLHGGPGNFDVASFLFADNPDGVSANIADGTATGEGDDTLVGIESLQGSGGPDV
                     LVGDAQFNVFYPMEGDDSVFGGPGGDLVSFIFAPRAVTADVFFGQATGEGTDSLVSIS
                     DFQGSRFGDTLTGDPFENQLFGEAGNDTLSGGASDDLLDGAEGTDTGDGGLPGPEGDR
                     CFSIEHPTNCEITERRGGARQLSVLEELLGRVERLLR"
     gene            complement(1669..2805)
                     /locus_tag="VG709_01605"
     CDS             complement(1669..2805)
                     /locus_tag="VG709_01605"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWC31807.1"
                     /translation="MRIAVLGAGAMGSAAARLLARRTDTEILVLDADEERARGVTADV
                     GLGEARSVDLRDTSASNALMGADAVAVCVPYRLNLEAMELAVAARVPYADLGGLYHMT
                     LRQLELDARFREAGVPAVLGVGCCPGISNVFARLGAERLDDVRSVDLLDGSIERDAGF
                     GVPYSADTILDEFVAAAMVFEGGELKEVPAGSGAVRFRFPDPLGEMEAVYTLHSELAT
                     LPRTIRGVRDVRWRLALPPKVADGFRLLVDLGLASEDPIDTTAGPVVPRELLHRLLAR
                     VPQPDGPPRDVEVLVAIVRGVKEGRPATFSAWATFRPTPEGISAGAFGTAIPIVVAAR
                     WLAEGRIPPGVHPPESVLDPDAFLEDLAREGVELRLELEERLTG"
     gene            2833..4302
                     /locus_tag="VG709_01610"
     CDS             2833..4302
                     /locus_tag="VG709_01610"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC31808.1"
                     /translation="MADQEVRVPNQMFLAGRWTDGSGDGTIDVRSPATGESLGSVPVS
                     SERDVDGAVRGAREGLAAMEAMTFFERAALLHRAADVMEERKEELGRLVSLEQGKPLR
                     TEGVAEIEESAENFRVAAEDVKRLTSEVLPSADRNKRMFTFRKPNGVYAVITPWNFPF
                     TIPSELVAPALAGGNAVVFKPSEFTPLIGLKMVQILEEAGFPRGSVSLVFGERTTGEA
                     LVTHDGVDAIAFVGSHETGEAIVRAAGLKRTLMELSGNGPQIVLDDANLDAAASLATF
                     GATYVSGQCCVATERVLVAESVHDEFVERLMKEAGSVVLGDPLEERTTMGPLNNRPVA
                     EKMDRHVADARERGVDILRGGRRGEGYPTDLYYELTVADRVGTDTLLFREESFGPVVP
                     ITTFSTDDEAIRLANDSQLGLQAAVFTSSLRRAFEMIERLNVGNVVVNDTTDYWESLE
                     PFGGASGTRTGWGRVGGKWGLMDMTDLRTAVIDYRNTRD"
     gene            complement(4310..4382)
                     /locus_tag="VG709_01615"
     tRNA            complement(4310..4382)
                     /locus_tag="VG709_01615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4347..4349),aa:Arg,seq:tct)
BASE COUNT          701 a         1529 c         1624 g          616 t
ORIGIN      
        1 cgccggcttc ctccatcccc cgacgacgcc tccacggagg cgccagagcg tcgcgacgat
       61 cgcggccagc ccgacggcct ggacgccggc gagaaggtcg ccgcggaacc ggaacccccc
      121 gacctcgaag tacacccacg cgagcgccgt cacgatcacg aacgcgggaa ggaagaacgc
      181 gacgccggcg aggatgccgc cgaggggccc ggcgcgcagg tatcccagat ggatcgcgac
      241 ctcgctcgag ttgggcccgg ggatcagatt cgtgacgccg acgacctcga ggaagtggcg
      301 ctcgtcgacc cagcgccgcc ggcgcacgag gtcgtcgtgc atcgccgcga tgtgggcgtt
      361 cggacccccg aacccgatcg tcccgagccg aaggaagtac cggacgacct ctccgtatcg
      421 ccccacggcc cgcggactat accgagtagc cattgacgga ggaccgcctc cgcctccggg
      481 acgattttcc gggcatacct gggcataggc cgccgctgcg ctggtcgaat ggcgtcggtc
      541 gtgctctcat ggagccgtga cgaagcggct cgtatccgtc atcgcggcca cgtccgtcgt
      601 ggcgtggatc gtcctgtcgc cgggtgcggc cggcgccgcg cggccgaagt gcaaggggaa
      661 gcgcgcgacg atcgtcggca cgaaccgcag cgagcggatc gtggggacgc ctcggcgcga
      721 cgtgatcgtt gccaagggtg ggcgcgacgt gatcctggcg cggaaccggg cggatctcat
      781 ctgtgcgggg cccggaaacg acttcgtgcg ggcgggcgga ggcaacgacg ccgtctacgg
      841 acagctcgga gccgacatcc tcgcgggaga cgccggtgct gacgtgatcg caggcgccgc
      901 gggagcagag ctcctgtatg ggggcgacgg caacgacacg atgcgcgccg ggaccggcgc
      961 ttcgtccgtc atgtacggca accgcggcaa cgaccgcatg ttcggcgggg acggattcga
     1021 cacgctccta gggctcgagg gcaacgaccg cctccacggc gggccgggca acttcgatgt
     1081 cgcatcgttc ctgttcgccg acaatcccga cggcgtgtcg gcgaacatcg cggacggaac
     1141 cgccacggga gagggcgacg acacgctcgt ggggatcgag agcctccagg gctcgggagg
     1201 gcccgacgtc ctggtcggcg acgcccagtt caacgtcttc tacccgatgg agggcgacga
     1261 ctcggtattc ggcggcccgg gtggcgacct cgtgtcgttc atcttcgcgc cgcgtgccgt
     1321 cacggccgac gtgttcttcg gccaggcgac cggcgagggc accgactcgc tggtcagcat
     1381 ctccgacttc cagggctcgc ggttcggcga cacgttgacg ggggatccgt tcgagaacca
     1441 gctgttcggc gaagcgggga acgacacgct ctccggcggc gcctcggacg acctgctcga
     1501 tggcgcggaa ggaaccgata ccggcgacgg cgggctcccc ggccccgagg gcgaccgttg
     1561 cttcagcatc gagcacccga ccaactgcga gatcactgag cggcgcggcg gtgcgcgaca
     1621 gctgtcggtg ctcgaggagc tcctcgggcg ggtcgaacgc ctcctccgtt agccggtcag
     1681 ccgttcttcg agctccaagc gcagctccac gccctcgcgc gccaggtctt ccaggaacgc
     1741 atcggggtcg agcacgctct cgggcgggtg gacgcccggc gggatccgtc cctcggctag
     1801 ccagcgcgcc gcgaccacga tcggtatcgc ggtcccgaac gccccggccg agatcccctc
     1861 gggggtcggc ctgaacgtcg cccacgccga gaaggtcgcg ggtcgtccct ctttcacgcc
     1921 acgcacgatc gcgacgagca cttcgacgtc gcgcggcgga ccgtcgggct gcgggacgcg
     1981 cgcgaggagg cggtggagga gctcgcgcgg caccacgggc cctgcggtcg tgtcgatcgg
     2041 gtcctccgac gccagcccga ggtcgacgag gagccggaac ccgtctgcca ccttcggcgg
     2101 aagcgcgagc cgccaccgca cgtcgcgcac gccgcggatc gtgcgaggga gcgtcgcgag
     2161 ctcggagtgg agcgtgtaga ccgcctccat ctcgccgagc gggtccggaa agcggaagcg
     2221 gaccgcgccg gaccccgcgg ggacctcctt cagctcgccg ccctcgaaga ccatcgccgc
     2281 cgcgacgaac tcgtcgagga tcgtgtcggc ggagtacggg acgccgaatc ccgcatcgcg
     2341 ctcgatcgag ccgtcgagga ggtcgacgct ccggacgtca tcgagccgct cggcgcccag
     2401 gcgggcgaag acgttggaga tgccgggaca gcacccgacg ccgagcacgg ccggcacgcc
     2461 ggcttcccgg aaccgcgcgt cgagctccag ctgccggagc gtcatgtggt acaggcctcc
     2521 gaggtccgcg tacgggacgc gcgcggcgac ggcgagctcc atcgcctcga ggttgaggcg
     2581 gtaagggacg catacggcga ccgcgtcggc acccatgagg gcgttcgagg cgctcgtgtc
     2641 ccgcaggtcg accgaccgcg cctcgccgag accgacgtcg gctgtgacgc cccgtgcccg
     2701 ttcctcgtcg gcgtcgagca cgaggatctc cgtgtcggtt cgacgcgcta ggaggcgtgc
     2761 cgccgcgctg cccatcgccc ccgcaccgag cacggcgatc cgcatcgcgc gagcataccg
     2821 gtagtagcct gaatggccga tcaggaggtg cgtgtgccga accagatgtt ccttgcgggc
     2881 aggtggaccg acggatctgg tgacgggacg atcgacgtcc ggagccccgc gaccggggag
     2941 tcgctcggat cggttccggt ctcgagcgaa cgcgacgtcg acggggcggt ccgcggtgca
     3001 cgcgagggcc tcgcggcgat ggaggcgatg acgttcttcg agcgcgccgc gctcctgcac
     3061 cgcgccgccg acgtgatgga ggagcggaag gaggagctcg ggcggctcgt ctcgctcgag
     3121 caggggaagc cgctacggac ggaaggggtc gccgagatcg aggagtcggc cgagaacttc
     3181 cgcgtcgcgg ccgaggacgt gaagcggctg acgtccgagg tgctcccgtc cgccgaccgc
     3241 aacaagagga tgttcacgtt ccgtaagccg aacggcgtct acgccgtgat cacgccgtgg
     3301 aacttcccgt tcacgatccc gtcggagctc gtcgcgccgg cgctcgcggg cggtaacgcc
     3361 gtggtgttca agccgtcgga gttcactcca ctcattggcc tcaagatggt gcagatcctc
     3421 gaggaggcgg ggttcccgcg cggctccgtc tcgctcgtct tcggcgagcg cacgaccggc
     3481 gaggcgctcg tcacgcacga cggcgtcgac gcgatcgctt tcgtcggctc gcacgagacc
     3541 ggcgaggcga tcgtgcgcgc cgccggcctg aagcggacgc tcatggagct ctcgggcaac
     3601 ggaccgcaga tcgtgctcga cgacgccaac ctcgacgcgg cggcctcgct cgcgacgttc
     3661 ggcgcgacgt acgtctcggg gcagtgctgc gtcgcgaccg agcgtgtcct cgtcgccgag
     3721 tcggtccatg acgagttcgt cgagcggctg atgaaggagg cgggaagcgt cgtcctgggc
     3781 gatccgctcg aggagcggac cacgatgggc ccgctcaaca accggccggt ggccgagaag
     3841 atggaccgcc acgtcgcgga cgcgcgcgag cgcggcgtcg acatcctccg aggagggcgc
     3901 cgcggcgagg gctacccgac cgacctctac tacgagctca cggtcgccga tcgcgtcggc
     3961 accgacacgc tgctgtttcg cgaggagtcg ttcgggccgg tcgtaccgat cacgacgttc
     4021 tcgaccgacg acgaggcgat caggctcgcg aacgactcgc agctggggct ccaggcggcg
     4081 gtgttcacgt cgagcctgcg ccgggcgttc gagatgatcg agcggctcaa tgtcgggaac
     4141 gtcgtcgtca acgacaccac cgactactgg gagtcgctcg agccgttcgg cggcgcgtcg
     4201 ggcacacgga cggggtgggg ccgtgtcggc ggcaagtggg gcctcatgga catgaccgac
     4261 cttcgcaccg ccgtgatcga ctatcgcaac acgagggact gaaggaccgc gcgccgggga
     4321 ggattcgaac ctccgacccg ctgcttagaa ggcagccgct ctgtcctctg agctaccggc
     4381 gccgccatca atgatagggg agggtgggga gccgccccgt agaatggctg gcgtggcgaa
     4441 cgaccaacca cgggggcggc ccgccggcgt
//
LOCUS       DASZOB010000101         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_194124, whole genome shotgun sequence.
ACCESSION   DASZOB010000101 DASZOB010000000
VERSION     DASZOB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194124"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VG709_01620"
     CDS             <1..829
                     /locus_tag="VG709_01620"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWC31809.1"
                     /translation="LGSFDRLVTSHALSLLAVELGKSREVAEAERRLKGDFLDSLVRE
                     QLGEAEVARGLRRFGLDPGGEVKVIAITSDVSLEDLAWATEDVLSRTRGGFLTAPRDD
                     ALYVVVRPDGDRELTDLHDAVVERVGTEVCVAAGSPVPPAEVGRSLREARYALRVCKV
                     EDRTIADFGSLGTYRLLLSLQEPDALRAFADSVLAPLERYDAERGGDLIESLRAFLEH
                     NARWETASAELFVHRHTLRYRMRKIEELTGRDLGSPHDRMEFWLALRARELLVRTPD"
     gene            949..2406
                     /locus_tag="VG709_01625"
     CDS             949..2406
                     /locus_tag="VG709_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWC31810.1"
                     /translation="MATTLQRRQMFIGGQWVDAADERTVAVVNPATGDVIAETPQASE
                     EDVDRAVRAAQKAFDETWFDTTPGDRQAMLLKLADAIEENAEELGRIESENVGKPTSW
                     VVSDEISVVADQLRFFAGAARVVTGLAAGEYYKEGSTLYTSWLRREPVGVVGLIAPWN
                     YPLYMAMWKFAPALAAGNTVVIKPSEWTPLTLLRTVELAQDIFPPGVLNVVTGEGVPT
                     GAAIVRHPGVGMVSLTGEVTTGKEVARTAAETLKRVHLELGGKAPVVVFDDANLESSV
                     EWIKTAGYFNSGQDCTAATRVLAGSGIYDSLLSELVPAVESMKVGDPSSDDTEMGPVV
                     AQAQLERVSGFVDRARDGGATVLTGGERIAGRGFFYKPTVVTDVGQDAEIVQNEVFGP
                     VVTVQRFDGDDQAIAWANDVDYGLAASVWTRDVGRALNASRKLRFGTVWVNTHIPLTP
                     EMPHGGFKQSGYGKDMSIYSIEHYTELKHVMAPID"
     gene            2416..>3667
                     /locus_tag="VG709_01630"
     CDS             2416..>3667
                     /locus_tag="VG709_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC31811.1"
                     /translation="MSDTRPFLVAGEWRTGAETFDVTSPFDGSVVARVGSPSDDDVED
                     ALRAADDTFDETRRLPVHARAEALAHISRRIAERAEEFADAIAREGGKPLKWAKVEAT
                     RAVSTFRWAAEELRTHRDELIRIDTDPALGSRVGLVRRFPYGPVLGIAPFNFPVNLVA
                     HKLAPALAVGAPISVKPATKTPIGALLLAELFGETDLPAGMLAVLPLQGSRTEPLVED
                     ERIKKVSFTGSSPVGWKLKSLDPKKRFTLELGGNAGAIVHSDADLDHAAARIAFGGYY
                     QAGQSCIAVQRVLVHAPVYDDFLTRFVKQVESLKVGDPLDPETDVGPVIDGGALERID
                     EWVREALDEGAEALVGGSRDDPFFEPTILGNVKREMKVCREEIFGPVTSVLRYETFEE
                     ALEQANDSPYGLQAGVFTSDIERAF"
BASE COUNT          555 a         1213 c         1358 g          541 t
ORIGIN      
        1 gctcggaagc ttcgaccgtc tcgtgacgag ccacgcgctc tcgctgctcg cggtcgagct
       61 cgggaagtcg cgggaggtcg ccgaggcgga gcggcgcctg aagggcgact tcctcgactc
      121 gctggttcgc gagcagctcg gcgaggcgga ggtcgcgcgg gggctccggc gcttcgggct
      181 cgaccccggc ggcgaggtga aggtgatcgc gatcacgtcc gacgtatcgc tcgaggacct
      241 cgcctgggcg accgaggacg tcctgtcgag gacccgcggc gggttcctca ccgcaccacg
      301 agacgacgcg ctctacgtcg tggtgcgccc cgacggcgat cgcgagctga ccgacctcca
      361 cgacgccgtg gtcgagcgcg tcgggaccga ggtctgcgta gccgccggca gcccggtgcc
      421 gccggcagag gtggggcgga gcctccgtga ggcgaggtac gcgctcaggg tctgcaaggt
      481 ggaggaccgc acgatcgccg acttcggctc gctcgggacg taccggttgc tgctctcgct
      541 tcaggagccc gacgcgctcc gcgcgttcgc ggactcggtc ctggcgccgc tcgagcggta
      601 cgacgccgag cgcggaggcg acctcatcga gtcgctccgg gcgttcctcg agcacaacgc
      661 tcgctgggaa acggcgtctg ccgagttgtt cgtccaccgc cacacgctgc gctaccggat
      721 gcgcaagatc gaggagctca ccggacgcga cctcggctcg ccgcacgatc gcatggagtt
      781 ctggctggcg ctgcgggcgc gtgaattgct ggtgcggacg cccgattgac ccggggggcg
      841 gttgtcccaa ccgtacatgt ggcgaggcgc cgtatcggcc gggacgagaa cccgcgttcc
      901 tcgaccggtt cgctaggctc ggggccgtaa gcgggtgagg aggatctgat ggcgacgacg
      961 ctccagcgca ggcagatgtt catcggcggt cagtgggtgg acgcggcaga cgagcgaacc
     1021 gtcgcggtcg tgaatcccgc gacaggagac gtgatcgccg agacgccgca ggcgtcggag
     1081 gaggacgtcg accgcgcggt ccgcgcggca cagaaggcct tcgacgagac ctggttcgac
     1141 acgactcccg gtgaccgaca ggcgatgctc ctcaagctgg cggacgcgat cgaggagaac
     1201 gccgaggagc tggggcggat cgagtccgag aacgtcggca agccgacctc gtgggtcgtc
     1261 tccgacgaga tctcggtggt cgcggaccag ctgcggttct tcgccggcgc ggcgcgtgtc
     1321 gtgaccggcc tcgcggccgg cgagtactac aaggaggggt cgacgctcta cacgagctgg
     1381 ctccgccgag agccggtcgg cgtcgtcggc ctcatcgcgc cctggaacta cccgctgtac
     1441 atggcgatgt ggaagttcgc ccccgcgctc gcggcgggga acaccgtcgt catcaagcct
     1501 tccgagtgga cgccgctcac gctgctccga accgtcgagc tcgcgcaaga catcttcccg
     1561 ccgggtgtgc tgaacgtcgt gacgggggag ggcgtgccga cgggcgctgc gatcgtgcgg
     1621 catcccggcg tcggcatggt atcgctcacc ggcgaggtca cgaccggcaa ggaggtcgcc
     1681 aggaccgcgg ccgagacgct gaagcgcgtc cacctcgagc tcggggggaa ggcgccggtc
     1741 gtcgtgttcg acgacgcgaa cctcgagtcg tcggtggagt ggatcaagac cgccggttac
     1801 ttcaactcgg ggcaggactg cacggcggcc acgcgggtgc tggccggctc cggcatctac
     1861 gacagcctgc tctcggagct cgtgccggcc gtcgagtcga tgaaggtggg ggacccatcg
     1921 tcggacgaca ccgagatggg ccccgtcgtc gcccaggcgc agctcgagcg cgtttcgggt
     1981 ttcgtggacc gtgcgcgcga cggcggcgcg acggtgctca cgggcggcga gcggatcgcc
     2041 ggccgcgggt tcttctacaa gccgacggtc gtcaccgacg tcgggcagga cgccgagatc
     2101 gtgcagaacg aggtcttcgg gccggtcgtg accgtgcaga ggttcgacgg cgacgaccaa
     2161 gcgatcgcgt gggcgaacga cgtcgactac ggcctcgccg cgtcggtgtg gactcgtgat
     2221 gtgggccgcg cgctgaacgc gtcgcggaag ctgcggttcg ggaccgtgtg ggtcaacacc
     2281 cacatcccgc tcactccgga gatgccgcac ggcggattca aacagtccgg gtacggcaag
     2341 gacatgagca tctactcgat cgagcactac accgagctca agcacgtgat ggccccgatc
     2401 gactgaggtg acccgatgag tgacaccagg cccttcctcg tggcggggga gtggcggacg
     2461 ggcgccgaga cgttcgacgt caccagcccg ttcgacggct ccgtcgttgc gcgggtcggc
     2521 tcgccgtccg acgacgacgt cgaggacgcg ctccgcgcgg ccgacgacac gttcgatgag
     2581 acgcgccggc ttcccgtgca cgcgcgcgcg gaggcgctcg cgcacatctc gcggcgcatc
     2641 gcggagcggg ccgaggagtt cgccgatgcg atcgcgcgcg agggcggcaa gccgctgaag
     2701 tgggcgaagg tcgaggcgac gcgcgccgtc tcgacgttcc ggtgggccgc cgaggagctc
     2761 cggacgcacc gcgacgagct gatccggatc gatacggatc ccgcgctcgg gagccgcgtc
     2821 gggctcgtgc gccgcttccc gtacggaccc gtcctcggga tcgcgccgtt caacttcccg
     2881 gtgaacctcg tcgcgcacaa gctcgccccc gccctcgcgg tcggcgcgcc catcagcgtg
     2941 aagccggcga cgaagacgcc gatcggtgcg ctcctcctgg cggagctctt cggggagacc
     3001 gacctgccgg ccgggatgct cgcggttctc ccgctccagg gcagccggac cgagcctctc
     3061 gtcgaggacg agcggatcaa gaaggtgtcg ttcaccggct cgagcccggt cgggtggaag
     3121 ctcaagtcgc tcgaccccaa gaagcggttc acactcgagc tcggcgggaa cgccggcgcg
     3181 atcgtccatt cggatgcaga cctcgaccac gcggccgcgc ggatcgcgtt cggcggttac
     3241 taccaggccg gccagtcctg catcgcggtc cagcgcgtgc tcgtgcacgc gccggtctac
     3301 gacgacttcc tgacgcggtt cgtgaagcag gtcgagagcc tcaaggtcgg cgacccgctc
     3361 gacccggaga cggacgtggg cccggtgatc gacggcggcg ccctcgagcg gatcgacgag
     3421 tgggtgcgcg aggctctcga cgagggcgcg gaggctctcg tcggcgggtc gcgggacgat
     3481 ccgttcttcg agccgacgat cctggggaac gtgaagcgtg agatgaaggt gtgccgcgag
     3541 gagatcttcg gcccggtgac ctcggtgctg cggtacgaga cgttcgagga ggcgctcgag
     3601 caggccaacg actcgccgta cgggcttcag gccggcgtct tcacgtcgga catcgagcgg
     3661 gcgttcc
//
LOCUS       DASZOB010000102         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_62915, whole genome shotgun sequence.
ACCESSION   DASZOB010000102 DASZOB010000000
VERSION     DASZOB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62915"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VG709_01635"
     CDS             <1..535
                     /locus_tag="VG709_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31812.1"
                     /translation="RVTEYYQRAGENSARIVYFRGMLWGTAALAGLVGGVLALGWTLG
                     LLDPRAEPTYTLFVSVAMGAVGAILSVMTRMASPNGFSLEFEVGRKSVRYLGGLRPWI
                     GAMFAFVLYLALKRNLVELVQASDHGVYFYGTLAFAAGFSERRAKVLLDGALGGSGSD
                     RDGDAKATAGSPARTTV"
     gene            614..1009
                     /locus_tag="VG709_01640"
     CDS             614..1009
                     /locus_tag="VG709_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31813.1"
                     /translation="MRRSRWEYKVFLVALAVMFLHLTEDTLVHEESGSSVGAKLGATV
                     LNLLLAAVGAVVYPLLWRRARPLLVLAYGALGLVAGWRAHVTDLLDGDAAGGDYTGTL
                     FTLAGLVLVGLAVKLAVDTMRDRRAPASG"
     gene            1146..>1504
                     /locus_tag="VG709_01645"
     CDS             1146..>1504
                     /locus_tag="VG709_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWC31814.1"
                     /translation="MTGAEGNGTAASKDLLWFIAEGTAGTVGEEFFQCLVKHLARAFR
                     ADVAFVAEVMPDDPRRARFLACWEGGRLVPEPFEYDMAGTPCNELGDSHVVSYPEGVM
                     ERFPGDEMVVDLGLDSYL"
BASE COUNT          192 a          525 c          533 g          254 t
ORIGIN      
        1 gcgtgtcacg gagtactacc agcgcgcggg cgagaactcg gcgcgcatcg tctacttccg
       61 cggcatgctg tggggaaccg cggccctcgc cggcctcgta ggcggtgtcc tcgcactggg
      121 gtggacgctc ggcttgctcg atcctcgagc cgaaccgacg tacacgctct tcgtcagcgt
      181 cgcgatgggc gcagtcggcg ccattctgag cgtcatgacg cgaatggcgt cgcccaacgg
      241 cttcagcctc gagttcgagg tgggcaggaa gtcggtccgc tacctcggcg gcttgcggcc
      301 gtggatcggc gcgatgttcg ccttcgtcct ctatctcgcc ctcaagcgca acctcgtcga
      361 gctcgtgcag gcgtccgacc acggcgtcta cttctacggc acgctggcgt tcgccgcggg
      421 cttcagcgag cggcgcgcga aggtcctgct cgacggcgcg ctgggcggct cgggctccga
      481 tcgcgacggc gacgcgaagg cgaccgccgg ctccccggcg cgtacgacgg tctaagccct
      541 acggcgacgt aggtcggcgc tggggacagt gcgcgttcta gcggcgcagg agtcgaatcg
      601 gcccagcgac gggttgcgcc gctcccgctg ggagtacaag gtcttcctcg tcgcgctcgc
      661 cgtcatgttc ctccacctga ccgaggacac cctcgtccac gaggagagcg gctcgtcggt
      721 cggtgcgaag cttggcgcca ccgtgttgaa cctgctgctc gcggccgtcg gcgccgtggt
      781 ctatccgctg ctctggcgcc gcgcgcggcc gcttctcgtc ctggcgtacg gggcgctcgg
      841 actcgtcgcc ggttggcgcg cccacgtcac cgacctgctc gacggcgatg ccgcgggcgg
      901 ggactacacc gggacgctct tcacgctcgc cggcctcgtg ctcgtcgggc tcgccgtcaa
      961 gcttgccgtc gacacgatgc gcgaccgccg cgcgccggcc tccggttagc cgtgccggcg
     1021 aagaccggat atgggccgat cggcctattg cggtgtcccg cgcggcgtgg caacgtacag
     1081 gcttcgtgca cggaagcgca ttctcagagc gctcgaacgg gggacggcac ggcggggaca
     1141 tcggcgtgac cggcgccgaa ggcaacggga cggcggcctc gaaggacctc ctctggttca
     1201 tcgcggaggg taccgccggc accgtcggcg aggagttctt ccagtgcctc gtcaagcacc
     1261 tcgcgcgcgc gtttcgcgcc gacgtcgcgt tcgtcgccga ggtcatgccg gacgacccgc
     1321 gccgcgcccg ctttctcgcg tgctgggagg gcggcaggct cgttcccgag ccgttcgagt
     1381 acgacatggc ggggacgccg tgcaacgagc tcggggactc tcacgtcgtc tcctacccgg
     1441 agggcgtcat ggagcggttc ccgggcgacg agatggtcgt cgatctcggg ctcgacagct
     1501 acct
//
LOCUS       DASZOB010000103         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_103176, whole genome shotgun sequence.
ACCESSION   DASZOB010000103 DASZOB010000000
VERSION     DASZOB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103176"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VG709_01650"
     CDS             complement(<1..1045)
                     /locus_tag="VG709_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC31815.1"
                     /translation="MAPVSRAARAGPPAANVPRRVPSKLFAKPGEFVESCVPEALVYF
                     LLNVGDGDTQLVLLPARASGGRRALVVDVATTRKLPRLLEALGAARLLVPLPDDVLFP
                     LVVATHPHNDHIGGMPEFLAGYGRYVREFWEPGYYNPIGGYIETMRVLEDADGAIQHT
                     QPTSGTTRFIGKVKVQVLSPSIALRNRFDSYGIDINNSSIALKLEFPASRVQWHKQGR
                     RYVRVGERQGRLILGADAQTRAGGKVMDDFPALQESRSEAAKALRMALGTDPLRADVF
                     KIPHHGSKHGVNLELVEEIRPAYSLVSSVGGGGEFNFPHLVAVEAVREAREPTTSSGS
                     RHKPDWELGIHFTG"
     gene            complement(1032..1604)
                     /locus_tag="VG709_01655"
     CDS             complement(1032..1604)
                     /locus_tag="VG709_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31816.1"
                     /translation="MSPAAREWTPDWLRAEEDANAFDLSWPLWRPTGVAEADPRELAY
                     IVVDDLADDTAVLAVSVWPRLDPERRLRFPVDERPRLVRVETDELMSILREQRRRFAD
                     RPLRIGDVFAARASLPNLPPPASRAEQAQLEAEEPPPGSLAWLEPPVYDLTAQGREAA
                     KAALAAAVSPLLSDDDVRALTAEERSRGTR"
     gene            complement(1777..>2005)
                     /locus_tag="VG709_01660"
     CDS             complement(1777..>2005)
                     /locus_tag="VG709_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31817.1"
                     /translation="PGPGMTAGREEGRAEVYLMCEDVERTRRALEAKGVEFGDPVTDE
                     GWGLLTRLNVPGFGGLGLYEPRHPSPLAAFD"
BASE COUNT          273 a          731 c          700 g          301 t
ORIGIN      
        1 ctcccgtgaa gtggatcccg agctcccagt cgggcttgtg ccggcttccg ctcgacgtcg
       61 tcggctcccg ggcctcacgg accgcttcca cggcgacgag gtgcgggaag ttgaactccc
      121 cgccgccgcc gaccgaggag acgagggagt acgccggcct gatctcctcg accagctcga
      181 ggttgacccc gtgcttggac ccgtggtggg ggatcttgaa gacgtccgcc cgcagcggat
      241 cggtcccgag cgccatcctg agcgccttcg ccgcctccga gcggctctcc tggagcgcgg
      301 ggaagtcgtc catcaccttg ccccccgcgc gcgtctgggc gtcggcgccg aggatgagcc
      361 ggccctgccg ctcgccgacg cggacgtagc gtctaccctg cttgtgccac tgcacgcggg
      421 aggcggggaa ctcgagcttg agcgcgatcg acgagttgtt gatgtcgatc ccgtagctgt
      481 cgaagcgatt tcgcagcgcg atcgagggcg agagcacctg caccttcacc ttgccgatga
      541 agcgggtcgt tccgctggtc ggctgcgtgt gctggatcgc tccgtccgcg tcctcgagga
      601 cgcgcatcgt ctcgatgtat ccgccgatgg ggttgtagta gcccggctcc cagaactcgc
      661 ggacgtaccg cccgtaaccg gccaggaact cgggcatgcc gccgatgtgg tcgttgtgcg
      721 ggtgcgtcgc gacgacgagc gggaagagga cgtcgtcggg cagcggcacc agcaggcggg
      781 ccgcgcccag tgcctcgagc agccgtggca gcttccgcgt cgtagcgacg tcgacgacga
      841 gcgcgcgccg accgccgctc gccctcgcgg ggagcagtac gagctgcgta tcgccgtcgc
      901 cgacgttcaa caggaagtag accagcgcct cggggacgca gctctcgacg aattccccgg
      961 gcttggcgaa gagcttgctc gggacgcgcc tcgggacgtt ggccgccggc gggcccgccc
     1021 gtgcagcgcg gctaacgggt gccacgagac ctctcctcgg ccgtcagggc ccggacgtcg
     1081 tcgtcgctga gcagcggcga tacggccgcc gccagcgccg ctttcgccgc ctctcgcccc
     1141 tgcgcggtca agtcgtagac cggcggctcg agccacgcga gcgatcccgg cggcggctcc
     1201 tcggcctcga gctgcgcctg ctcggcgcgg cttgccggcg gcggaaggtt gggaagagag
     1261 gcgcgcgccg cgaagacgtc gccgatccgc agcggacggt cggcgaaccg acggcgttgt
     1321 tcacgcagga tcgacatgag ctcgtccgtc tcgacgcgca cgagccgggg gcgctcgtcg
     1381 accgggaacc gcagcctcct ctcggggtcg agccgaggcc acacggacac cgcgagcacc
     1441 gcggtgtcgt ccgcgagatc gtccacgacg atgtacgcga gctcgcgcgg atccgcctcg
     1501 gcgaccccgg tcggcctcca gagcggccac gacaggtcga aggcattggc gtcttcctcg
     1561 gcccgaagcc agtcgggcgt ccactcgcgc gcggccgggc tcatcagacg cccttcaccg
     1621 cccgcgtcga ccgttcgttg ccgcaccccg tggatcggcg ctgtgcgacg gttcgacgag
     1681 cggatcgacg cggctctcca cgatgacgat tgtctcgcat cctgacgcgc gtctccagcg
     1741 gcatccatcg gccctccgcg acctcttcgc ggaacctcag tcgaacgccg cgagcgggct
     1801 cgggtggcgc ggctcgtaca ggccgagccc gccgaagccg ggcacgttca gccgcgtcag
     1861 caggccccac ccttcgtcgg tgacggggtc gccgaactcg acgcccttcg cctcgagtgc
     1921 gcggcgggtc cgctcgacgt cctcgcacat gaggtagacc tcggcgcgcc cctcctcgcg
     1981 ccccgcggtc atcccgggcc cggga
//
LOCUS       DASZOB010000104         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_153633, whole genome shotgun sequence.
ACCESSION   DASZOB010000104 DASZOB010000000
VERSION     DASZOB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153633"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..327)
                     /locus_tag="VG709_01665"
     CDS             complement(4..327)
                     /locus_tag="VG709_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HWC31818.1"
                     /translation="MWLPLLGLGLGVAVGLLLQINVSPELARYSAVGILAGLDSVLGA
                     IRAELEAHYDNRVFLSGFVMNALVAVALTFVGDRLGIDLYLVALFAFGLRIFQNVALI
                     RRHFL"
     gene            complement(333..1064)
                     /locus_tag="VG709_01670"
     CDS             complement(333..1064)
                     /locus_tag="VG709_01670"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWC31819.1"
                     /translation="MLTGGFVYALMGPLFRVETLASSGTRFTPTEAIDARLAEVNGSS
                     MLTLDTRALANGIEALPGVAEARIETTLPGTVSVAVREDEPVIVWSTATAFLAIASDG
                     GVVGAFSDADSLPADVAGLPLIEDSRGSTGDIGVGDRLDAVETRAALRLATVDPALLG
                     SDVPALSVRVDDLYGFVLVAPERWEAALGFYGREPGDDAEAATARIERQIAAVRTLFA
                     DQPEASVVWLDARNPGKIYFRATEG"
     gene            complement(1208..>1567)
                     /locus_tag="VG709_01675"
     CDS             complement(1208..>1567)
                     /locus_tag="VG709_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HWC31820.1"
                     /translation="EVVSAHEWYDYEAKYVPGLAEVAPKADVEPALAGELKALALAAY
                     RAVDCAGLARVDFLVPSGGVYISEMNTIPGFTATSMFPKQAELAGIPLPELIDRLIRL
                     AIERKAGSATGDTVGVT"
BASE COUNT          261 a          546 c          546 g          214 t
ORIGIN      
        1 agcctacagg aagtggcgac gtatcagtgc gacgttttgg aagatgcgca gcccaaacgc
       61 gaacagggcg acgaggtaca ggtcgatccc gaggcggtcg cccacgaagg tcagcgccac
      121 ggcgacgagc gcgttcatca cgaagccgga gaggaagacg cggttgtcgt aatgcgcctc
      181 gagctcggcg cgtatcgcgc ccagcacgga gtcgaggccg gcaaggatgc cgaccgcgct
      241 gtagcgggcg agctccgggg acacgttgat ctgaagcagc agaccgaccg cgacgccgag
      301 ccccagcccg agcaggggga gccacataaa attcagccct cggtggcacg aaaatatatc
      361 tttcccgggt tccgggcgtc gagccacacg acggacgcct cgggctggtc cgcaaacagg
      421 gtgcgcaccg cggcgatctg gcgctcgatt ctcgcggttg cggcctcggc gtcgtcgccc
      481 ggctcgcggc cgtagaagcc gagcgcggcc tcccagcgtt ccggagcgac gagcacgaac
      541 ccatacagat cgtcgacccg gaccgagagc gcgggtacgt ccgagcccag gagggccgga
      601 tcgacagtcg cgagcctgag ggccgcgcgt gtctccaccg catcgagccg atcacccact
      661 ccgatatcgc ccgtggaccc ccgggagtcc tcgatgagcg ggaggcctgc gacatcggcc
      721 gggagcgagt ccgcgtcgct gaatgcgccg acgaccccgc cgtcggaggc gatggcgagg
      781 aacgccgtcg cggtgctcca gacgatgacc ggctcgtcct cccgtaccgc gacgctgacg
      841 gtcccgggga gcgtcgtctc gatgcgcgcc tccgcgactc cggggagcgc ctcgataccg
      901 ttcgcgagcg cgcgcgtgtc gagcgtcagc atgctcgacc cgttgacctc ggcgagtcgc
      961 gcgtcgatcg cctccgtcgg ggtgaagcga gtcccgctgg acgcgagcgt ttcgacacgg
     1021 aagagcggac ccatcagggc gtagacgaag cccccggtga gcacgaccgc gcacaggagc
     1081 gcgagacccc gcgcacggcc ggggaggcgc ctgcggatcc tcgtccgcag tggcacaccg
     1141 ggcttgcggg cctgtgctcg ccggcgagcg cgctgcggtt cgaccagccg tgcgccggaa
     1201 ccgcgggtca cgtcaccccg accgtgtcac cggtggcgct cccggccttc cgctcgatcg
     1261 ccaggcggat gagcctgtcg atcagctcgg gaagcgggat tccggcgagc tccgcctgct
     1321 tcgggaacat gctcgtcgcc gtgaagccgg gaatggtgtt catctcggag atgtagacgc
     1381 ccccggacgg caccaggaag tcgaccctcg cgaggcctgc gcagtcgacc gctcgatagg
     1441 cggctagcgc gagcgccttg agctcgccgg cgagcgccgg ttcgacatcg gccttcggcg
     1501 ccacctccgc cagcccggga acgtacttcg cctcgtagtc gtaccactca tgcgcgctca
     1561 cgacctc
//
LOCUS       DASZOB010000105         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_194241, whole genome shotgun sequence.
ACCESSION   DASZOB010000105 DASZOB010000000
VERSION     DASZOB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194241"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VG709_01680"
     CDS             complement(<1..1054)
                     /locus_tag="VG709_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31821.1"
                     /translation="MRRILVTTTLAVIAATLPAGAAVAADPPPNDDFANAEPIQGYRG
                     STPGDNRGATGEPGEPENARGTTPINSIWYRWTAPAGGPVVIDTCGSDPYFDTTLGVY
                     IVGHFPQVISTVATNDNDSGCVGFQSKVQFSAVAGVTYHISVDGWWHYTGSVVLRWAL
                     GPPENDDFADADVIEGADGSVRGSNEHATGEPGEPDNAGVSKPIESVWYRWTAPYSGT
                     AMFHTCSSRFDTTLGVYTGTRVDGLTEVASNDDACWIRSSVEFSADAGTTYHISVDGW
                     ESHEGTPVLSWYLQEHDAPTSSASSPAYTAARTFAVDYTASDAGSGVFQVELWARGPT
                     DARFSFVAIQWGETGSF"
     gene            complement(1255..2700)
                     /locus_tag="VG709_01685"
     CDS             complement(1255..2700)
                     /locus_tag="VG709_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HWC31822.1"
                     /translation="MVIDRRTFLQRSIAAAAGTFTLAGPFRGLVARAAAASPGVAANN
                     AGYGPLQPVPDRRDGVVRLHLPEGFQYRSFSYTGERMADGVTTPGRHDGMAAFPGPSG
                     TVRLVRNHEVNGPGPAFGDASRAYDPMARGGTTTVQLSEHAEKYRAWVSANGTMMNCA
                     GGEMPYGTWVTCEETVNGPDVGPDFTGAPNDPLHEKHGYIFEVPSSDDPNEYEERNPI
                     RSAGRFAHEAVALDPLTGFLYLTEDNFAFPSGYYRYRPPTNPMVTGRIADGGALEMLR
                     IPGKPRARLDKREDVGRSFNVDWVPIPNPDPTFPEGTTNDEALVAVSNQGLEQGAAIF
                     SRLEGLFYDEGVFYIVSTQGGQVDEGAPTSGFGEGLGQVWAYEPAREKLTLIFESPGP
                     DVLELPDNVAVTPRDGILLCEDGPADNYLRGLTPDGEIFDFALNAIEGRRGDEFAGAT
                     FSPSGDTLYVNIQASSGLSFAIWGPWTNGPL"
     gene            complement(2798..>2978)
                     /locus_tag="VG709_01690"
     CDS             complement(2798..>2978)
                     /locus_tag="VG709_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31823.1"
                     /translation="LEGRVEALEQRHRRLTERAADLRTRARTAERERDRLRERIRRVT
                     GEIRELEADAAALRE"
BASE COUNT          401 a         1042 c         1059 g          476 t
ORIGIN      
        1 ggaacgagcc ggtctccccc cactggatcg cgacgaagga gaaccgcgcg tccgtcggcc
       61 ctcgggccca cagctcgacc tgaaagacgc cagagccggc gtcggaagcc gtgtagtcga
      121 cggcgaacgt gcgcgccgcc gtgtacgcgg gcgacgacgc ggaggacgtc ggagcgtcgt
      181 gctcctggag gtaccacgag agcaccggcg tcccttcgtg agactcccaa ccgtcgacgg
      241 agatgtggta cgtggtgcct gcgtccgccg agaactccac gctgctcctg atccaacacg
      301 cgtcgtcgtt gctcgcgacc tcggtgaggc cgtccacccg cgtccccgtg tagacgccca
      361 gcgtcgtgtc gaatcggctg ctgcacgtgt ggaacatcgc ggtccccgag tacggcgcgg
      421 tccaccggta ccacaccgat tcgatcggct tggagacgcc ggcgttgtcc ggctcgccgg
      481 gctctcccgt cgcgtgctcg ttcgagccgc gcacggatcc gtccgctccc tcgatgacgt
      541 ccgcgtcggc gaagtcgtcg ttctcgggcg gccccagtgc ccacctgagc accacggagc
      601 cggtgtagtg ccaccagccg tcgaccgaga tgtggtacgt gacccccgcg actgccgaga
      661 actgaacctt gctctggaag cccacgcacc cgctgtcgtt gtcgttggtc gccaccgtgc
      721 tgatgacctg cgggaagtgg ccgacgatgt agacgccgag cgtggtgtcg aagtacgggt
      781 cgcttccgca ggtgtcgatg acaaccgggc cgccggccgg cgcggtccac cggtaccaga
      841 tcgagttgat aggggttgtc cctctggcgt tctcgggctc tccgggctcg cccgtggctc
      901 cccggttgtc tccgggcgtc gatccacggt agccctggat gggttcggcg ttcgcgaagt
      961 cgtcgttcgg cgggggatcg gccgccaccg cggctccggc cgggagcgtc gcagcgatca
     1021 cggcaagggt ggtcgtgacg aggatccttc gcatcggcct cagcctcgac gttcggacac
     1081 aatggcggta cccgcgtgtg aggggggagc gatcccccaa tctgggggtg tcagcggtgg
     1141 accgccgacg ggatcgggca ggaggctgat gtgacccggg tcgcggcagg ccggagcaga
     1201 cgagcgatag cgcctacgcg ctcgcgaccg cgaggcttca ggctcgcgct aggtctacag
     1261 cgggccgttc gtccagggcc cccagatcgc gaacgacagg cccgacgacg cctggatgtt
     1321 cacgtagagc gtgtcgccgc ttgggctgaa cgtcgccccg gcgaactcgt caccgcggcg
     1381 cccctcgatc gcgttgagcg cgaagtcgaa gatctcgccg tcgggcgtga gcccgcgcag
     1441 gtagttgtcc gcggggccgt cctcgcacag caggatcccg tcccgagggg tgaccgcgac
     1501 gttgtcgggt agctcgagca cgtccggccc gggagactcg aagatcagcg tcagcttctc
     1561 gcgcgcgggc tcgtacgccc acacctggcc cagcccctcg ccgaagcccg acgtcggcgc
     1621 tccctcgtcg acctggccgc cctgcgtcga gacgatgtag aagacgccct cgtcgtagaa
     1681 caggccctcg agccggctga agatggcggc cccctgctcg aggccctggt tgctgaccgc
     1741 cacgagcgcc tcgtcgttgg tcgtcccctc cgggaacgtc gggtccgggt tggggatcgg
     1801 cacccagtcg acgttgaagc tgcgaccgac gtcctcgcgc ttgtcgagcc gcgcgcgcgg
     1861 cttcccgggg atgcggagca tctcgagggc gccgccgtcg gcgatgcggc cggtcaccat
     1921 cgggttcgtc ggcgggcgat accggtagta gccggatggg aacgcgaagt tgtcctcggt
     1981 gaggtagagg aaccccgtga gggggtcgag cgcgaccgcc tcgtgcgcga accggccggc
     2041 gctccggatc gggttgcgct cctcgtactc gttcggatca tcggaggagg gcacctcgaa
     2101 gatgtagccg tgcttctcgt ggagcgggtc gttcggcgct cccgtgaagt cggggccgac
     2161 gtcgggcccg ttcacggtct cctcgcacgt gacccacgtg ccgtacggca tctcgccgcc
     2221 ggcgcagttc atcatggtcc cgttcgcgct gacccacgcg cggtacttct cggcgtgctc
     2281 ggagagctgc acggtcgtgg tgccgcctcg cgccatcggg tcgtacgctc ggctcgcgtc
     2341 gccgaacgcc ggcccggggc cgttgacctc gtggttgcgg acgagccgca cggtccccga
     2401 cgggcccggg aacgccgcca tgccgtcgtg ccgccccggc gtcgtgacgc cgtcggccat
     2461 ccgctcgccg gtgtagctga acgagcggta ctggaacccc tcaggaaggt ggagccgcac
     2521 gacgccgtcg cggcggtcgg ggaccggctg gagcggcccg tagcccgcgt tgttcgcggc
     2581 gaccccgggc gacgccgcgg cggcccgagc gacgaggccg cggaacggcc cggcgagcgt
     2641 gaaggtccca gctgcggcgg cgatgctgcg ctgcaggaac gtgcgacggt cgatcaccat
     2701 cgggcgccct ccttatgtcc cggcttctcc accccgacgg cgggactgta agcccgcgcc
     2761 cggctcatcc tcaaatcggc cgatccccga acggtcgtta ctcgcggagg gcggcggcgt
     2821 cggcctcgag ctcgcggatc tcgcccgtga ctcgccggat ccgctccctg agtcgatcgc
     2881 gctcgcgttc cgcggtgcgg gccctcgtgc ggagatccgc cgcacgttcc gtgagacggc
     2941 ggtggcgctg ctcgagcgcc tcgacgcggc cctcgagt
//
LOCUS       DASZOB010000106         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_153715, whole genome shotgun sequence.
ACCESSION   DASZOB010000106 DASZOB010000000
VERSION     DASZOB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153715"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VG709_01695"
     CDS             <1..238
                     /locus_tag="VG709_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31824.1"
                     /translation="HYLFTYNTGTEATIEKDDYVRAFQGEGFVITDQGRTRGINYTRL
                     AGNEEIVVWSNDVLMAVVEGPFDVAAGFFLELPY"
     gene            303..1466
                     /locus_tag="VG709_01700"
     CDS             303..1466
                     /locus_tag="VG709_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC31825.1"
                     /translation="MDFDLTPEQEQFRRAVREFSEEVVAPRCEEMDRAEELPLDIVKQ
                     MGELGLFGLPFPEEYGGQDADLTTLCLAIEELARIDSSIAITLEAAVGLGANPVFHHG
                     TEEQKQRWLVPMCRGEILGSFGLTEPGGGSDAASTRTSAKLEGGEWVINGSKAFITNS
                     GTDISKVCTITARTGQREHGTPEISAIIVPTDTPGFEVAPHYRKLGWRASDTHELSFN
                     DVRVPEDHILGERGRGFAAFLQTLDEGRVAIAARAVGLCQGALEQSVRYAKEREAFGK
                     PIGSFQGLQFKIADMKVRTETARLAVYQAAWLRDQGRPYKAEASLAKLHATEAAVTQA
                     RENVQVHGGYGFIEDFPAARVYRDAKVLEIGEGTSEVQRVLIARDLGLPNTEF"
     gene            1559..>1772
                     /locus_tag="VG709_01705"
     CDS             1559..>1772
                     /locus_tag="VG709_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31826.1"
                     /translation="MSRASGAPGKRPLRHAAGALLVMLVFAQAPGTVHASDTKKELDR
                     ARNQLRDLREEHKDVERILDRLGGQQA"
BASE COUNT          298 a          588 c          624 g          262 t
ORIGIN      
        1 ccactacctc ttcacctaca acaccggcac cgaggcgacg atcgagaagg acgactacgt
       61 ccgcgccttc cagggagagg gcttcgtcat cacggaccag ggtcggacgc gaggcatcaa
      121 ctacacccgc ctggcgggga acgaggagat cgtcgtgtgg agtaacgacg tcctcatggc
      181 cgtcgtcgag ggacccttcg acgtcgcggc cggcttcttc ctcgagctcc cgtactaatc
      241 gccggatcga cacggaaggg gctcctccgg gggccccttc cgttcgttta cgctgtgccc
      301 ggatggactt cgacctgacg ccggagcagg agcagttccg ccgggccgtc cgcgagttct
      361 ccgaggaggt cgtcgctccc cgttgcgagg agatggaccg ggcggaggag ctgccgctcg
      421 acatcgtcaa gcagatgggt gagctcgggc tgttcgggct ccccttcccg gaggagtacg
      481 gaggccagga cgcggacctg acgacgctgt gcctcgcgat cgaggagctc gcgcggatcg
      541 actcgtcgat cgcgatcacg ctcgaggcgg cggtcgggct cggcgcgaat ccggtgtttc
      601 atcacgggac cgaggagcag aagcagcggt ggctcgtccc catgtgccgc ggcgagatcc
      661 tcggctcgtt cgggctcacg gagcccggcg gcggctccga cgcggcgtcg acgaggacct
      721 cggcgaagct cgagggcggc gagtgggtga tcaacggctc gaaggcgttc atcacgaact
      781 ccgggaccga catctcgaag gtctgcacga tcaccgcgag gacggggcag cgggagcacg
      841 gaacgcccga gatctcggcg atcatcgttc ccaccgacac cccaggtttc gaggtcgcgc
      901 cccattaccg caagctcggg tggcgcgcgt ccgacacgca cgaactctcc ttcaacgacg
      961 tccgcgtgcc cgaggaccac atcctcggcg agcgtggtcg ggggttcgcg gccttcctgc
     1021 agacgctgga tgagggacgt gtcgcgatcg cggcgcgcgc ggtcggcctg tgtcagggcg
     1081 cgctggagca gagcgtgcgg tacgcgaagg agcgggaggc gttcgggaag ccgatcggct
     1141 cgttccaggg cctgcagttc aagatcgcgg acatgaaggt ccgcacggag acggcgaggc
     1201 tcgcggtcta ccaggcggcg tggctccgcg accaggggag gccgtacaag gcggaggcct
     1261 cgctcgcgaa gctccacgcg accgaagcag ccgtgaccca ggctcgcgag aacgtccagg
     1321 tccacggcgg gtacgggttc atcgaggact tccccgccgc gcgggtctat cgcgacgcca
     1381 aggtcctcga gatcggcgag gggaccagcg aggtccagcg ggtcctcatc gcccgcgacc
     1441 tcgggctgcc gaacaccgag ttctagcgcc ttccggtcgt ccgccggacg cgccgcagcc
     1501 gtcacgttcg tcacaaccgt tgacattcgt ctcacgcatg agatacaggt aagggttcat
     1561 gagcagagcg agcggcgctc cggggaagcg cccgcttcgg catgccgccg gagcgctcct
     1621 cgtgatgctc gtcttcgccc aggccccggg caccgtccac gcttcggata cgaagaagga
     1681 gctcgaccgc gcccgcaacc agctccggga cctccgcgag gagcacaagg acgtcgagcg
     1741 catcctggac cggctcgggg gtcagcaggc gg
//
LOCUS       DASZOB010000107         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_183865, whole genome shotgun sequence.
ACCESSION   DASZOB010000107 DASZOB010000000
VERSION     DASZOB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183865"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VG709_01710"
     CDS             <1..781
                     /locus_tag="VG709_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWC31827.1"
                     /translation="EFRRSYVLEQERAAQLEAAYAAERARVGDLERADRARARLLEIL
                     THELLNPVAAIRAATLVLQRRWDALPEETRREMLQRVSGQSEQLSHVAADASATLQEQ
                     APALSVEPRSVPVRRLLDDAVAGAAFLEDRLSLRVEDDAVEASVRADPPRVQQVFANL
                     LTNAARHGSPGGAVELAAEAVDGDWIRFSVKNPGPGIPEDQREQLFEAFTRLRDDRPG
                     SGLGLFICRRIVEAHGGRISADGGPGETVFAFTLPRESDRR"
     gene            778..1446
                     /locus_tag="VG709_01715"
     CDS             778..1446
                     /locus_tag="VG709_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC31828.1"
                     /translation="MSEGVGVLVVDDHPMFAQALEMLIEAEPDFRVVGVVGSGEEAVE
                     AASSSCPDVVLMDIDLPGMSGIEATRLLVGRCPDTRIVVISALQPDEVLLDAIEAGAG
                     GVISKTEAVDAILDVVRRASAGDVVLPPGDSLGLLRRLQMTRRDRSERDRRLDQLTDR
                     EVEILQRLAEAKTTDEVASALFISPHTVHAHMRSILRKLEARSKLEAVLLALRHGRVE
                     LPRP"
BASE COUNT          216 a          503 c          569 g          215 t
ORIGIN      
        1 ggagttcagg cggagctacg tgctcgagca agagcgggcg gcgcagctcg aggcggcgta
       61 cgcggcggag cgggcgcgcg tcggagacct cgagcgagcc gaccgagcgc gggcgcggct
      121 gttagagatc ctcacgcacg agctcctcaa cccggtggcg gcgatccgcg cggcgacgct
      181 ggtcctccag cgccggtggg acgcgctacc ggaggagacc cgccgggaga tgctccagcg
      241 ggtgagcggc cagtccgagc agctgtccca cgtcgcggcg gacgcatcgg cgacgttgca
      301 ggagcaggcc cccgccctct cggtcgaacc gaggagcgtg ccggtgcgcc gtctgctcga
      361 cgacgccgtg gcgggggcgg cgttcctgga ggaccggttg tcgctgcgcg tcgaagacga
      421 cgcggtggag gcgtcggtcc gcgccgatcc tccgcgcgtc cagcaggtgt tcgcgaacct
      481 cctgacaaac gccgcgcgcc atgggtctcc tggaggcgcg gtcgagctcg cggcggaggc
      541 cgtcgacggt gattggatca ggttcagcgt gaagaacccc ggtccaggca tcccggagga
      601 tcagcgcgag cagctgttcg aggcgttcac gcgcctgcga gacgaccggc ccggctccgg
      661 gctcgggctg ttcatctgcc gccggatcgt cgaggcgcac ggcggacgca tcagcgccga
      721 cggggggccc ggcgagacgg tgttcgcctt cacgctgcct cgcgaatcgg accggcgatg
      781 agcgagggcg ttggtgtgct ggtcgtggac gaccacccga tgttcgcgca ggcgctcgag
      841 atgctgatcg aggccgagcc cgacttccgg gtcgtcggcg tcgtcggctc gggcgaggag
      901 gcggtcgagg ccgcgagctc gagctgcccc gacgtcgtcc tcatggacat cgacctcccg
      961 gggatgagcg gcatcgaggc gacgcgcctc ctcgtcgggc gctgcccgga tacgcgcatc
     1021 gtcgtgatca gcgcgctcca gccggacgag gtcctgctgg atgcgatcga ggccggcgcg
     1081 ggcggggtga tctcgaagac cgaggccgtc gacgcgatcc tcgacgtcgt ccggcgcgcg
     1141 tccgcggggg acgtcgtcct gcctccgggc gactcgctcg ggctcctccg ccggctgcag
     1201 atgacacggc gggaccgcag cgagcgcgac cggcggctcg atcaactgac cgatcgcgag
     1261 gtcgagatcc tgcagcgtct ggcggaagcg aagaccaccg acgaggtcgc ctcggcgctg
     1321 ttcatcagtc cacacaccgt gcatgcgcac atgcggtcga tcctgaggaa gctggaagcg
     1381 cggtcgaagc tcgaggcggt cctgctagcc ctgcgtcacg gacgggtcga gcttcctcga
     1441 ccctaagcgt cgacctcgac ttcaggttct ctccgagcac ggatcgcgca gcccgggtgg
     1501 gat
//
LOCUS       DASZOB010000108         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_2519,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000108 DASZOB010000000
VERSION     DASZOB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VG709_01720"
     CDS             <1..396
                     /locus_tag="VG709_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HWC31829.1"
                     /translation="GTGSIGIEALSRGASSCVFVDQADAPVRTIRQNVTRTKLARRAR
                     VVRSDVRRFLRRTDDAFDLVFLDPPFAIAHAGLTETLQLAAERLDPGGTVVVSRPTRD
                     ATDVIPVNLRVATLLRYGDTTVLVCRGGE"
     gene            393..869
                     /gene="coaD"
                     /locus_tag="VG709_01725"
     CDS             393..869
                     /gene="coaD"
                     /locus_tag="VG709_01725"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWC31830.1"
                     /translation="MTLTALCPGTFDPVTNGHLDIVGRAARLFDRVVVAVVENPAKQP
                     MFPVEERMPMLKEAMGDMRSVEVDSFAGLLVDYARTREVDAIVKGLRAVTDFDYELQM
                     AQMNRHLSGVETCFVPTSPEWSYLSSSLVREVARYGGDVSRLVPAHVASAMKERLG"
     gene            869..1477
                     /locus_tag="VG709_01730"
     CDS             869..1477
                     /locus_tag="VG709_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31831.1"
                     /translation="MDIAGRLQQLEELIAQAKSMPLSASVLVNRDEALELVHEARDAL
                     PEEIKQARWVVKDRENLLAKARRDAEGIIQQALQEQARLVSREEVVQASGKEADRILA
                     EARDEARQIRLEAEDYMDQKLAAFEATLTRAQEQIDRIREHEDGMLARAGDQLGKIRE
                     QQDEDYAKIEEQLSRTLQQVQRGRERLRGGSIAEEELGAEEF"
     gene            1477..1968
                     /locus_tag="VG709_01735"
     CDS             1477..1968
                     /locus_tag="VG709_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWC31832.1"
                     /translation="MELVDVRDLLAAPAATRALKRSEALEGLEGELARADDPIRMELR
                     LESVVEGILVSGRLRGSIRYSCARCLKPGESGFDVEVTELFSQGAAPDDEEAYPVEEG
                     SIDLEPMVRDAVLLGMPFSPLCRPDCLGLCERCGGDRNLGECVCAEEVDPRWAALDGL
                     NID"
     gene            1979..2155
                     /gene="rpmF"
                     /locus_tag="VG709_01740"
     CDS             1979..2155
                     /gene="rpmF"
                     /locus_tag="VG709_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWC31833.1"
                     /translation="MAVPKRRTTKTRRDTRRAQWKGTPPTYSLCPQCRQPKLPHRVCK
                     NCGYYAGRQAIEVE"
BASE COUNT          376 a          757 c          843 g          315 t
ORIGIN      
        1 gggacgggct cgatcgggat cgaggcgctc tcccgcgggg cgagctcgtg cgtcttcgtc
       61 gaccaggcgg acgcgccggt caggacgatc aggcagaacg tcacgcggac gaagctggcg
      121 cgccgagcgc gcgtcgtccg gtccgacgtc cgccggttcc tgcggcggac cgacgacgcc
      181 ttcgacctcg tcttcctcga cccccctttc gcgatcgccc acgcggggct gaccgagacg
      241 ctccagctcg ccgccgaacg gctcgatccg ggcggaacgg tcgtcgtctc ccggccgacc
      301 agggatgcca ccgatgtgat tcctgtaaac ttgcgcgtcg cgacgctcct taggtacggc
      361 gacaccaccg ttctggtctg tcggggaggc gagtgaccct caccgcgctc tgcccgggaa
      421 ccttcgaccc ggtcacgaac ggccacctcg acatcgtcgg gcgggccgca cggctgttcg
      481 accgcgtggt cgtcgctgtg gtcgagaacc cggccaaaca gccgatgttc ccggtcgagg
      541 agcgcatgcc gatgctgaag gaagcgatgg gggacatgag aagcgttgaa gtcgactcgt
      601 tcgcggggct gctggtggac tacgcccgca cccgcgaggt cgacgcgatc gtgaaggggc
      661 tccgcgccgt caccgacttc gactacgagc tccagatggc gcagatgaac cggcacctgt
      721 ccggcgtcga gacgtgtttc gtcccgacga gtccggagtg gtcgtacctc tcgtcgtcgc
      781 tcgtccgcga ggtcgcgcgg tacggcggag acgtctcgcg cctggtcccc gcccacgtcg
      841 cgagcgcgat gaaggagcgg ctcggctgat ggacatcgcc ggccgactcc agcaactcga
      901 ggagctgatc gcgcaggcga agtcgatgcc gctctcggca tcggtgctcg tcaaccgcga
      961 cgaggcgctc gagctcgtcc acgaggcgcg cgacgcgctc cccgaggaga tcaagcaggc
     1021 gcggtgggtc gtgaaggacc gcgagaacct cctcgcgaag gcgaggcgcg acgcggaggg
     1081 catcatccag caggcgctcc aggagcaggc gcggctcgtc tcgcgcgagg aggtcgtgca
     1141 ggcgtccggc aaggaggccg accggatcct cgcggaggcg cgggacgagg cccggcagat
     1201 ccgcctcgag gccgaggact acatggacca gaagctggcc gcgttcgagg cgacgctgac
     1261 gcgggcccag gagcagatcg accgcatccg agagcacgag gacgggatgc tcgcgcgggc
     1321 cggggaccag ctcgggaaga tccgggagca gcaggacgag gactacgcga agatcgagga
     1381 gcagctctcg aggacgctcc agcaggtgca gcgaggccgg gagcgccttc ggggcggcag
     1441 catcgccgag gaggagctcg gcgcggagga gttctgatgg agctcgtcga cgtccgtgac
     1501 ctcctcgcgg ctccggcggc cacgcgcgcg ctgaagcggt cggaggcgct cgagggcctt
     1561 gagggcgagc ttgcgcgcgc cgacgatccg atccgcatgg agctccgcct cgagagcgtc
     1621 gtcgaaggga tcctcgtttc gggacgcctg cgcggctcga tccgatactc gtgcgcgcgg
     1681 tgcctgaagc ccggcgagag cggcttcgac gtcgaggtga ccgagctctt ctcgcagggc
     1741 gccgcccccg acgacgagga ggcgtacccc gtcgaagagg ggtcgatcga cctcgagccg
     1801 atggtccgcg acgccgtgct gctcgggatg ccgttctcgc cgctctgccg gcccgactgc
     1861 ctcgggctgt gcgagcgatg cggcggcgat cgcaatctcg gcgagtgcgt ctgcgccgag
     1921 gaggtcgacc cgcgatgggc ggcgctcgac ggcctgaaca tcgactgaag gagagaaggt
     1981 ggcggtacca aagagaagga ccacgaagac gagacgggac acgcgtcgcg cccagtggaa
     2041 gggcacgccg ccgacctaca gcttgtgccc gcagtgcagg cagccgaagc tgccacatcg
     2101 cgtttgcaag aactgcgggt actacgccgg gcgccaggcc atcgaggtcg agtaggagcc
     2161 gcggttgctc ggacgggcca gccggctgga gcgggccctc aagatccgtt tccgcgccgg
     2221 agagttcagg cggctggcgc tcgcgcaccg gtcgttcgcc ttcgagcacg gcgagaccga
     2281 gaccaacgaa c
//
LOCUS       DASZOB010000109         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_93180, whole genome shotgun sequence.
ACCESSION   DASZOB010000109 DASZOB010000000
VERSION     DASZOB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93180"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VG709_01745"
     CDS             <1..882
                     /locus_tag="VG709_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWC31834.1"
                     /translation="VPLALIARGEHLRAIRERGLRLESIAGDALVHPAIATADPGAVG
                     TVDAVLVATKTWQLPEAARTMRPLVGAETVVVPLLNGVEAPEVLTEALGAEPVLGGLS
                     GMFSFIAGPGHIRHVGAEPWVTLGELEGGLSERVRRLAAILERCRGLQVHPSEDIRVA
                     MWEKFMFITATGGVGAVTRSPYGAFRAVRETRDMVEAAMREVVAVARARGIGLSERAI
                     SERMRFIDSLEPHGTTSMQRDMDASKRSELDEWNGAVVRLGRERGVETAVHAYIYASL
                     LPRELRARGDLEFPEAR"
     gene            908..>1869
                     /locus_tag="VG709_01750"
     CDS             908..>1869
                     /locus_tag="VG709_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWC31835.1"
                     /translation="MPRVNEEIAELLQEYSDLISISGGDPFKARAYEKAARSVGGYHD
                     DLDRLDERGIGAIPNVGKSIVVKIREYLETGTIRALEELRQEIPDGVRELIAIPALGP
                     RKALVLHRELGIGSVDELAHALRDNRLAGLKGFGAKTEENLRRGIEQLRSAGERHHLG
                     VALDLAETLLGHLRRLHEVLRADYAGSLRRMAETIGDVDLLVAGTEPTPIMEAFCSHP
                     LVERVGARGDTKSSIRTTRGIQVDLRVIAPDVWGAALQYFTGSKAHNVRVRELAVRKG
                     LKLSEYGLFRAKSGRLVVSETEEEVYERLGMKWIPPTLREDRGEV"
BASE COUNT          293 a          636 c          674 g          266 t
ORIGIN      
        1 gtcccgctcg ctctcatcgc gcgaggcgag catctccgcg cgatccgaga gcgcggtctg
       61 cgtctcgaga gcatcgcggg ggacgccctc gtccaccccg cgatcgccac ggcagatccg
      121 ggcgcggtgg ggacggtcga cgcggtcctc gtcgcgacga agacctggca gctgccggag
      181 gccgcgcgga cgatgcgccc gctcgtcggc gccgagaccg tcgtcgtccc cctgctgaac
      241 ggcgtcgagg cgccggaggt gctcacggaa gcgctcggcg ccgagcccgt cctcggcggc
      301 ctcagcggga tgttctcctt catcgccgga ccggggcaca tcaggcacgt cggagcggag
      361 ccctgggtga cgctcggcga gctcgagggt ggcctgagcg agcgcgtccg gcgcctggcg
      421 gcgatcctcg agcgctgccg tggactccag gttcacccga gcgaggacat ccgcgtcgcg
      481 atgtgggaga aattcatgtt catcaccgcg acgggcggcg tcggcgccgt gacgcggtct
      541 ccgtacggcg ccttcagggc ggtgcgcgag acgcgcgaca tggtggaagc cgccatgcga
      601 gaggtcgtgg ccgtcgcccg tgcacgaggc atcgggctgt cggaacgggc catctcggaa
      661 cggatgcgat tcatcgactc gctcgagccc cacgggacga cgtcgatgca gcgggacatg
      721 gacgcaagca agaggtccga gctcgacgag tggaacggtg ccgtcgtccg cctaggccgc
      781 gagcggggcg tcgagaccgc cgtccacgcg tacatctacg ccagcctcct cccgcgggag
      841 cttcgcgcgc gcggtgacct cgagttccct gaggcgcgct gacgacgcga ccgtagactt
      901 ccagccgatg ccgcgggtga acgaggagat cgccgagctc ctgcaggaat attcggacct
      961 catctctatc tcggggggcg atcctttcaa ggcgcgggca tacgagaagg ccgctcgatc
     1021 ggtcggcgga taccacgacg acctcgaccg gctcgacgag agagggatcg gcgccatccc
     1081 gaacgtcggg aagtcgatcg tcgtcaagat ccgcgaatac ctcgagacgg gaacgatccg
     1141 cgccctcgaa gagctccgtc aggagatccc cgacggcgtg cgcgagctga tcgcgatccc
     1201 ggcgctcggc ccgaggaagg cgctcgtcct ccaccgggag ctcgggatcg gctccgtcga
     1261 cgagctggcg cacgccctcc gcgacaaccg cctggcgggg ctcaaggggt tcggggcgaa
     1321 gaccgaggag aacctccgtc gcgggatcga gcagctgcgt tccgcgggcg agcggcacca
     1381 cctcggggtc gcgctcgatc tcgcggagac gctgctcggg cacctccgtc gcctccatga
     1441 ggtgctccgc gccgactacg cgggatcgct acggcggatg gccgagacga tcggtgacgt
     1501 cgacctcctc gtcgccggga ccgagccgac gccgatcatg gaggccttct gctcgcatcc
     1561 gctggtcgag cgcgtcggtg cccgtggcga cacgaagtcg tcgatccgca cgacgcgcgg
     1621 catccaggtc gacctccgcg tgatcgcgcc cgacgtgtgg ggagcggcgc tccagtactt
     1681 caccgggtcc aaggcgcaca acgtccgcgt ccgtgagctc gccgtgcgca aggggctcaa
     1741 gctctccgag tacgggctgt tccgcgcgaa gagcgggagg ctcgtcgtct cggaaaccga
     1801 ggaagaggtc tacgagcggc tcggcatgaa atggatccct ccgacgctgc gcgaggaccg
     1861 cggggaggt
//
LOCUS       DASZOB010000110         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_11277, whole genome shotgun sequence.
ACCESSION   DASZOB010000110 DASZOB010000000
VERSION     DASZOB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11277"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VG709_01755"
     CDS             complement(<1..369)
                     /locus_tag="VG709_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC31836.1"
                     /translation="MRLYDAAIVGGGHNGLVCAAYLARAGRSVVVLERRPFVGGAAVT
                     EEPWPGFRVSTASYGVSLMQRRIVRDLGLTRFGYFVYPLDPAYFTACPDGRGLYVWDD
                     PRRAAEEVAKFSERDARAYLR"
     gene            460..1035
                     /locus_tag="VG709_01760"
     CDS             460..1035
                     /locus_tag="VG709_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWC31837.1"
                     /translation="MAVTHDLSGKRIAFLATDGVEQIELTEPWKAVEEAAGTPELVSL
                     EAGTIQGFEHHDKGETFTVDRAIGDVDARDYDGLVLPGGVINPDRLRTDERAVSFVRA
                     FFQHGKPVAAICHGPWTLVEADVVRGRNLTSWPSLETDIRNAGGTWVDEELGTDAGLV
                     TSRKPDDLKAFCAKAVEEFAEGAHEQQRKSA"
     gene            complement(1082..>2067)
                     /locus_tag="VG709_01765"
     CDS             complement(1082..>2067)
                     /locus_tag="VG709_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31838.1"
                     /translation="IERAAATRTALAERSAALERRDALRERVERLAEPFGGTLPDVDA
                     LEADAAHAADLERRLARLHADRDRLEESRADLQQCAVEIETSRRRAADIGEELAALAF
                     DPEEHARLEKARHESRRLLEAASAEERTASDVLKEAESRVRELEARLEQVREIGARVR
                     ELRDEARMIDRVSSLLEGFRTFLYGRIMPQISRDAEALFRDLTNHEYEDLRIDEDTLA
                     IEIADADRYYPIERFSGSEVDLANLALRVAISTHLSLMAGADVGLMVLDEVMAALDAE
                     RKDLFVQTIGKLSQRFHQLFVITHAEQVKDQFPAAIELRRIARRRSTAVLV"
BASE COUNT          323 a          720 c          729 g          295 t
ORIGIN      
        1 ccgcaggtac gcgcgcgcgt cacgctccga gaacttcgcg acctcctcgg cggcgcggcg
       61 cgggtcgtcc cagacgtaga ggcctcggcc gtcggggcac gcagtgaaat aggcggggtc
      121 gagcggatac acgaagtagc cgaaccgcgt gagtccgaga tcgcggacga tccggcgctg
      181 catgaggctc acgccgtagg acgcggtcga gacgcggaac cccggccacg gctcctcggt
      241 gacggccgcg ccgccgacga acggtcgccg ctcgaggacg accaccgagc gtcccgctcg
      301 cgcgagatac gccgcgcaca cgagcccgtt gtgaccgccg ccgacgatgg ctgcgtcgta
      361 tagacgcacg gcgcctatcg tagccgcccc cttgccgtta ccgtccgcgg cccgccgggt
      421 atcacctcga agggtcacgc ttcatccgag agaggagtga tcgcagtgac gcacgacctg
      481 agcggcaagc gaatcgcgtt cctcgcgacg gacggcgtcg agcagatcga gctcaccgag
      541 ccgtggaagg ccgtcgagga ggccgcaggg acaccggagc tcgtctcgct cgaggcgggg
      601 acgatccagg gattcgagca tcacgacaag ggcgagacgt ttaccgtgga tcgagcgatc
      661 ggcgacgtcg acgcccgcga ttacgacggg ctcgtcctgc cgggcggggt catcaacccc
      721 gaccgtctcc ggaccgacga gcgcgccgtc tcgttcgttc gcgcgttctt ccagcacggc
      781 aagccggtcg cagccatctg ccacggcccg tggacgctgg tcgaggccga cgtcgtccga
      841 ggaaggaacc tcacgtcgtg gccgagcctc gaaaccgaca tccgcaacgc cggcgggacg
      901 tgggtcgacg aggagctcgg caccgacgcg gggctcgtga cgagccgcaa gcccgacgac
      961 ctgaaggcgt tctgcgccaa ggccgtcgag gagttcgcgg aaggggcgca cgagcagcaa
     1021 aggaagtccg cgtaacgcat gacgacgacg gggccggcgg cgcggcgccg gtcccgtctc
     1081 gctaaacgag caccgcggtg cttcggcggc gggcgatcct gcgcagctcg atcgccgccg
     1141 ggaactggtc cttcacctgc tcggcgtgcg tgatcacgaa tagctggtgg aagcgctgcg
     1201 agagcttccc gatcgtctgc acgaacaggt ccttccgctc ggcgtcgagc gccgccatca
     1261 cctcgtcgag caccatgagg ccgacgtcgg cgccggccat aagcgacagg tgcgtcgaga
     1321 tcgcgacgcg cagcgccagg ttggccagat caacctccga gcccgagaag cgctcgatcg
     1381 ggtaatagcg gtcggcgtct gcgatctcga tcgcgagcgt gtcctcgtcg atgcggaggt
     1441 cctcatactc gtggttcgtg aggtcgcgga acagcgcctc ggcgtcgcgt gagatctgcg
     1501 gcatgatccg cccgtagagg aacgtccgga agccctcgag cagcgaggac acgcggtcga
     1561 tcatgcgcgc ctcgtcgcgg agctctcgga cgcgcgctcc gatctcgcga acctgttcga
     1621 gccgggcctc gagctcgcgc actcgcgact ccgcttcttt gagcacatcg gaagccgtgc
     1681 gttcctcggc gctcgccgcc tcgagcagcc gcctcgactc gtggcgggcc ttctcgaggc
     1741 gcgcgtgctc ctcagggtcg aacgcgagcg cggcgagctc ctcgccgatg tcggcggctc
     1801 tccgcctcga cgtctcgatc tcgacggcgc actgctggag atcggcgcgc gactcttcga
     1861 gccgatcgcg gtcggcgtgg agcctcgcca ggcgacgctc gagatccgcg gcgtgggcgg
     1921 cgtcggcctc gagcgcgtcg acgtcgggca gggtgccacc gaacggctcc gccagccgct
     1981 cgacgcgctc ccgcagcgcg tcgcgtcgct cgagcgcggc acttcgttcg gccagcgcgg
     2041 tccgggtcgc cgcggcgcgc tcgatgg
//
LOCUS       DASZOB010000111         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_52820, whole genome shotgun sequence.
ACCESSION   DASZOB010000111 DASZOB010000000
VERSION     DASZOB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52820"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>2356
                     /gene="pepN"
                     /locus_tag="VG709_01770"
     CDS             175..>2356
                     /gene="pepN"
                     /locus_tag="VG709_01770"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWC31839.1"
                     /translation="MSRDNLTREEARGRAELIGDIRYDVTLDLTGEELFPSATIVDFR
                     CSQPGAETFIDFTGAEVASIELNGRTVGIDAFVGRRIRLTELEEENRLRILATAENST
                     TGKGIVRTVDPVDGRAYLYTDLEPFDAHRFFPCFDQPDLKGTFSFAALAPAEWHVLSN
                     MAAREPEDRDGKRLWTFEATPPIPTYIAVVVAGPYHGVRDRHGDIDLGVWCRESLAEH
                     LDADEILEITKQGFSFFEKAFRSPYPFHKYDQVFVPDFRAGAMENAGCVTFNEQRYVF
                     RSKVTGAEREWRAGTITHEMAHMWFGNLVTMRWWHDLWLNESFATYAGTLAQAEATRF
                     REAWASFAGYEKTWAYTQDQLPTTHPISADVPDTDSIHLNFDGITYAKGASVLKQLVA
                     WVGKERFLDGMALYFRRHQWGNASLRDFLVVLEEVSGRDLGQWSKEWLETAGVVTLRP
                     RVVTSGDRYTEVAIEQRAPEDHPTLRSHRIAVGLYDAGDEGLVRRRRVELDVVGDLTI
                     VADLAGEPVADLLLVNDDDLTYAKIRFDDRSLRTLGERLGDIREPLSRTLCWAACWDM
                     TRDAEMPARDYVDLVLRHAERETNPEVLRHLLQQATSAVTRYGDPGNRDAGLAKLADG
                     ALRALEQAAPASDEQLTWTRAFISAASTGHQLATVRGLLDGSTSFDGLAVDTELRWHI
                     VQSLAAAGADDAEELIAEEQRRDPTDLGARHAETARASAEPREAK"
BASE COUNT          376 a          822 c          819 g          339 t
ORIGIN      
        1 gcggctctac gccgaacgga gccgcgcctc gatcctcatc gaccggcaga agctcggcgg
       61 actccagctc ctcgcgttcg ggacgccggg gctaccggca ccggccgccg cgatcggcga
      121 ccgcgagacc ggctgctgag ggtccgcgtc ggagccgcgg gtaggatgac cggcatgtcg
      181 agggacaacc tcacgcgcga agaagcgcgg ggccgtgccg agctgatcgg agacatccgg
      241 tacgacgtca ccctcgacct caccggcgag gagctcttcc cgtcggcgac gatcgtcgac
      301 ttccgctgtt cgcagcccgg cgccgagacg ttcatcgact tcaccggcgc cgaggtggcg
      361 tcgatcgagc tcaacggccg aaccgtcggg atcgacgcgt tcgtcgggcg ccgcatccgc
      421 ctcactgaac tagaggagga gaaccggctg cgcatcctcg cgaccgcgga gaacagcacg
      481 accgggaagg gcatcgtccg aaccgtcgat cccgtcgacg gcagggcgta cctctacacc
      541 gacctcgagc cgttcgacgc gcaccgtttc ttcccgtgct tcgaccagcc cgacctcaag
      601 ggcacgttct cgttcgcggc gctcgcaccg gccgagtggc acgtgctgtc gaacatggcg
      661 gcgcgcgagc cggaggaccg ggacgggaag cggctgtgga cgttcgaggc gacacctccc
      721 atcccgacgt acatcgcggt cgtcgtcgcc ggtccctatc acggcgtccg cgaccgccac
      781 ggcgacatcg acctcggcgt gtggtgccgc gagtcgctgg ccgagcacct cgacgcggac
      841 gagatcctcg agatcacgaa gcaggggttc tcgttcttcg agaaggcgtt ccgttccccg
      901 tacccgttcc ataagtacga ccaggtgttc gttccggatt tccgtgccgg cgcgatggag
      961 aacgccggat gcgtcacgtt caacgagcag cgctacgtct tccgctcgaa ggtgacgggc
     1021 gcggagcgcg agtggcgcgc cggcacgatc acccacgaga tggcacacat gtggttcggc
     1081 aacctggtca cgatgcggtg gtggcacgat ctctggctca acgagagctt cgccacctac
     1141 gcgggcacgc tggcacaggc cgaggcaacg aggttccgag aggcgtgggc gagcttcgcg
     1201 gggtacgaga agacgtgggc gtacacgcag gaccagctgc cgacgacgca tccgatctct
     1261 gccgacgtcc ccgacaccga ctcgatccat ctgaacttcg acggcatcac gtatgcgaag
     1321 ggtgcgtcgg tcctgaagca gctcgtggca tgggtcggca aggagcggtt cctcgacggg
     1381 atggcgctgt acttccgccg ccaccagtgg gggaacgcct cgctgcgaga cttcctcgtc
     1441 gtcctcgagg aggtgtccgg ccgcgacctc ggccagtggt ccaaggagtg gctcgagacc
     1501 gcgggcgtcg tcacgctgcg gcctcgcgtc gtcacgagcg gcgatcggta cacggaggtc
     1561 gcgatcgagc agcgggctcc ggaggatcac ccgacgctac gctcgcaccg catcgcggtc
     1621 gggctgtacg acgcgggcga tgaagggctc gttcgccggc gtcgcgtcga gctcgacgtc
     1681 gtgggcgacc tcacgatcgt cgcggacctg gccggcgagc cggtcgccga cctcctcctc
     1741 gtcaacgacg acgacctcac gtatgcgaag atccggttcg acgaccgctc gctccggacg
     1801 ctcggcgaac gcctcggcga catccgcgag ccgctctctc ggacgctgtg ctgggcggcg
     1861 tgctgggaca tgacgcgcga cgccgagatg ccggcccgcg actacgtcga tctcgtgctt
     1921 cgccacgccg agcgcgaaac gaacccggag gtcttgcgcc acctcctcca gcaggcgacg
     1981 tcggccgtga cgcgctacgg cgatccgggg aaccgcgatg ccgggctcgc gaagctcgcg
     2041 gacggcgcgc tgcgcgcgct cgagcaggcc gccccggcga gcgacgagca gctgacgtgg
     2101 acgcgcgcgt tcatctccgc ggcctcgacg ggtcaccagc tcgcgacggt ccgagggctc
     2161 ctcgacggct cgacgtcgtt cgacggtctc gcggtcgaca ccgagctccg gtggcacatc
     2221 gtccagtcgc tcgccgccgc gggagccgac gacgcggagg agctcatcgc ggaggaacag
     2281 cggcgcgacc ccaccgacct cggggcgcgg cacgcggaga cggctcgcgc gtcggccgag
     2341 ccgcgcgagg cgaagg
//
LOCUS       DASZOB010000112         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_93241, whole genome shotgun sequence.
ACCESSION   DASZOB010000112 DASZOB010000000
VERSION     DASZOB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93241"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..959)
                     /gene="ligD"
                     /locus_tag="VG709_01775"
     CDS             complement(93..959)
                     /gene="ligD"
                     /locus_tag="VG709_01775"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWC31840.1"
                     /translation="MAETSVRFTNLEKELWPKSGVSKGELVAYLDAVRHAVLPHLRER
                     PLSVIRFHGEDDRGFFQKDLPKYAPDWVKSVRMYAPSAKRHVRYPLCNDRRTLLWFAN
                     QRAVELHAALARRDKPDRPTDLVLDIDPPAGAFDRAAEVARLLRAVLADLGLAARLKT
                     SGGKGLHVIVPLRRTHTFDDVRAASLAVAHRAEQRAPDLVTTEISKKKRGGRVLIDWT
                     RSQPAQTLIAPLSPRGRPGLPVSFPVAWDELSSVDPRDFTIATVPRIVGDGDPWTELS
                     PPPQSLPRTLTA"
     gene            1014..>1596
                     /locus_tag="VG709_01780"
     CDS             1014..>1596
                     /locus_tag="VG709_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWC31841.1"
                     /translation="MSADISVPANLEWLRRTESGAAWLASLPTLFAECADRWSLEVGD
                     PFPYTFASLAAPATTSDGTPVVLKIHFPDRESEHEARALEVWNGDGAVRLLAHDADRR
                     ALLLERCVPGTPLWEIGAQAALDVMVELLPRLWKPAGPPIRPLAEEAAWWASYLPDTW
                     ERAERPFERRLLDAALDALEVLPPTQSEQVLVHQ"
BASE COUNT          232 a          548 c          591 g          225 t
ORIGIN      
        1 cgcggcggcg ggcgcctcga cggtcggcgg gcgtccgtcg gcgacgaagc gacgatagat
       61 gtggaggcgg acgtcgcggt cgagatcggc cgtcacgcgg tcagcgtacg cgggagcgac
      121 tgcggcggtg ggctcagctc cgtccaggga tcgccgtcgc cgacgatgcg tgggaccgtc
      181 gcgatcgtga agtcgcgcgg gtcgacgctc gagagctcgt cccaggcgac ggggaacgag
      241 actgggagcc ccggtcgacc ccgaggactc agcggggcga tcagcgtctg cgcaggctgc
      301 gagcgggtcc agtcgatgag cacgcggccg ccgcgcttct tctttgagat ctcggtcgtg
      361 acgagatcgg gcgcgcgctg ctcggccctg tgcgcgacgg cgagcgaggc ggcgcggacg
      421 tcgtcgaacg tgtgcgttcg tctcaatggc acgatgacgt gcaggccctt tcccccagac
      481 gtcttcagcc gcgcggcgag tccgaggtcg gcgagaaccg cccgcagtag gcgcgcgacc
      541 tcggcggccc gatcgaacgc tccggccggc gggtcgatgt cgagcaccag gtccgtcggt
      601 cgatccggct tgtcgcgacg cgcgagcgcc gcatggagct cgacggcgcg ctggttcgcg
      661 aaccacagca gcgtccgacg gtcgttgcac agcggatagc gaacgtgccg cttcgccgac
      721 ggcgcgtaca tgcggacgga cttgacccaa tcaggggcgt acttcggcag gtccttctgg
      781 aagaaaccgc ggtcgtcctc cccgtggaag cggatgacgg acagcggacg ttcgcggaga
      841 tgcgggagca ccgcgtggcg aacggcgtcg aggtacgcga cgagctcgcc cttcgagacc
      901 ccgctcttgg gccagagctc cttctcgagg ttcgtgaacc gcacgctcgt ctccgccaac
      961 ggaccgttct accagggaac gggcgcggcg cgcctcgcca tacactcggc gcgatgtcgg
     1021 ccgacatctc ggtccccgcc aacctcgagt ggctccgcag gacggaatcc ggagcggcgt
     1081 ggctcgcgtc gctcccgacg ctgttcgccg agtgtgcgga ccggtggtcg ctcgaggtcg
     1141 gcgatccgtt cccgtacacg ttcgcgtcgc tcgcagcgcc cgcgaccacg tcggacggca
     1201 cgccggtcgt cctgaagatc cacttccccg accgcgagag cgagcacgag gcgcgcgcgc
     1261 tcgaggtctg gaacggagac ggcgcggtgc gcctcctcgc gcacgacgcc gaccgacggg
     1321 cgctgctgct cgagcggtgc gtgcccggca cgccgctgtg ggagatcggc gctcaggcgg
     1381 cgctggacgt gatggtcgag ctcctcccgc gactctggaa gcccgccgga ccgccgatcc
     1441 ggccgctcgc cgaggaggcg gcatggtggg cgagctacct ccccgacacg tgggaacgcg
     1501 ccgaacgacc gttcgagcgg aggctgctcg acgcggcgct cgacgccctg gaggtcctgc
     1561 caccgacgca gagcgagcag gtgctcgtcc accagg
//
LOCUS       DASZOB010000113         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_63251, whole genome shotgun sequence.
ACCESSION   DASZOB010000113 DASZOB010000000
VERSION     DASZOB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63251"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VG709_01785"
     CDS             complement(<1..491)
                     /locus_tag="VG709_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWC31842.1"
                     /translation="MITRVLVATDRSDSADTAVRWAADMANRYEAELVVLQVVLPRAE
                     HGEGDGQVEPATHHVLSDLATFAKELAGDRGHARVVVDEDPARAIVQMSEDADVDAVV
                     VGNLGMSGRTSFLLGNVPNRVSHNARCDVIIVNTARAVSPEREGRAVPIPTLPHIAEE
                     EAEV"
     gene            complement(503..748)
                     /locus_tag="VG709_01790"
     CDS             complement(503..748)
                     /locus_tag="VG709_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31843.1"
                     /translation="MDEVDDRLQDALATFEELAHETKPPEAWRNLSEPTVEKLWQAWP
                     DIRAWGEWMFQLIDGERRDKATPVPPEAELDETGGGG"
     gene            809..2179
                     /locus_tag="VG709_01795"
     CDS             809..2179
                     /locus_tag="VG709_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWC31844.1"
                     /translation="MPNRLATATSPYLLQHADNPVDWYEWGEEALERARREGKAILLS
                     VGYAACHWCHVMERESFEDEETARLMNEHFVCVKVDREERPDVDAIYMDAVQAMSGHG
                     GWPMTVFLTPDGEPFYAGTYFPPEDRFGMPGFKRVLTAVAEAWRERRNDVVQQGKQVV
                     EAIGRAAAASTEPLTEDLMRAAHAGLRRAFDDEWGGFGQAPKFPQPMTLEFLLRSHVR
                     GYDGALEIVTRTLDRMAAGGIYDQIGGGFHRYSTDRVWLVPHFEKMLYDNAQLATLYL
                     HAWQVSGNDRYREVVSETLEYLLREMRHEDGGFFSSQDADSEGVEGKFYVCPGTSSST
                     SSAKRRRATSAGRRKATGKAPTSSRATRRRPFRRTSALVCWTCGAGACGPEPTTRSSW
                     RGTRSRSRRSPRPGACCVRSDGSRPPSGPPTSSSRACDDATTAACFGRGAMGERAAPR
                     TPTTTR"
     gene            2179..2784
                     /locus_tag="VG709_01800"
     CDS             2179..2784
                     /locus_tag="VG709_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31845.1"
                     /translation="MAAACLTLYETTFDTRWFTEARALADDLLRLFHDGDGGGFFQTG
                     IDAERLVLRPKELFDNAVPSGNSAAAEVLLRLAFLTGEGSYESAAVSALRLVRDYLER
                     APTGFGNALAALDRYVGPSKEIAIVGDLASDEAGDLVETVWREYRPKIVMAAGSPDRG
                     DVVPLLADRTTVDGGPAAYVCERFACRMPVSDPEALTAQLD"
     gene            complement(2807..3202)
                     /locus_tag="VG709_01805"
     CDS             complement(2807..3202)
                     /locus_tag="VG709_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWC31846.1"
                     /translation="MKERAQELKAEARRGRGAGKADGERDVLAKIAEMTQPDRAMAER
                     LHAIVKATVPALSPRTWYGMPAYANQEGKVVCYFTAASKFKERYATFGFNAAANLDEG
                     AMWPTSFALKELTAAEEARIAALVKKAVS"
     gene            complement(3410..>3847)
                     /locus_tag="VG709_01810"
     CDS             complement(3410..>3847)
                     /locus_tag="VG709_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWC31847.1"
                     /translation="PVNTAALLGEAYSALGHRIVAGVAAAGFPQRPAHSSVFAHIDVE
                     GGTRLTELAGRANITPQAMGELVDDLERMRYVERRPDPEDRRAKRIVLTRRGIACVEA
                     AFGTIAVIERDLEGLLGSRGVKQLRSSLNRIIGAEGVEGERRV"
BASE COUNT          548 a         1339 c         1338 g          622 t
ORIGIN      
        1 acttccgcct cctcctcggc gatgtgcggc agcgtcggga tcggcaccgc gcggccctcg
       61 cgctccggcg agaccgcgcg cgcggtgttg acgatgatca cgtcacaccg cgcgttgtgc
      121 gacacgcggt tcgggacgtt gccgagcagg aagctcgtgc gtccgctcat cccgaggttc
      181 ccgacgacga cggcgtccac gtcggcgtcc tccgacatct ggacgatcgc gcgtgcggga
      241 tcctcgtcga cgacgaccct cgcgtggccg cggtcgccgg ccagctcctt cgcgaacgtc
      301 gcgagatcgg agaggacgtg gtgcgtcgcc ggctcgacct ggccgtcgcc ctcgccgtgc
      361 tcggcacgcg gcaggactac ctggagcacg accagctcgg cctcgtaccg gttcgccatg
      421 tcggcggccc accggacggc cgtgtcggcg ctgtcggagc ggtcggtcgc gacgaggacg
      481 cgggtgatca acgaaccgac ccctatcccc cgccacccgt ctcgtcgagc tcggcctccg
      541 gaggcaccgg cgtggccttg tcgcgcctct caccgtcgat gagctgaaac atccactcgc
      601 cccacgcgcg gatgtccggc catgcctgcc agagcttctc gacggttggc tcgctgaggt
      661 tccgccacgc ctccggcggc ttcgtctcgt gcgcgagctc ctcgaacgtc gcgagcgcgt
      721 cctggagcct gtcgtcgacc tcgtccatgt cgatccctcc gaggcggcca gtctacggcg
      781 cgaccagcgg tgggtaacac tggaggtgat gcccaaccgt ctcgcgaccg ccacgagccc
      841 gtacctcctc cagcacgccg acaatcccgt cgactggtac gagtgggggg aggaggcgct
      901 cgagcgcgcc cgtcgcgaag ggaaggcgat cctcctgtcc gtcggctacg cggcatgcca
      961 ctggtgtcac gtgatggagc gcgagtcctt cgaggacgag gaaaccgcgc gcctcatgaa
     1021 cgagcacttc gtgtgcgtga aggtggatcg tgaggagcgc cccgacgtcg acgccatcta
     1081 catggacgcc gtacaggcga tgagcgggca cggcgggtgg ccgatgactg tgttcctcac
     1141 gccggacggc gagccgttct acgccggcac gtacttccct ccggaggacc gcttcgggat
     1201 gccggggttc aagcgggtgc tcaccgcggt ggccgaggcg tggcgcgagc gccgcaacga
     1261 cgtggtgcag caagggaagc aagtcgtgga ggcgatcgga cgcgcggcgg cggcctccac
     1321 cgagccgctc accgaggacc tcatgcgcgc cgcgcacgcc gggctgcgtc gcgcgttcga
     1381 cgacgaatgg ggcggcttcg ggcaagcgcc gaagttcccg cagccgatga cgctggagtt
     1441 cctgctccgg tcccacgtcc gcggatacga cggagcgctc gagatcgtga cgaggacgct
     1501 cgaccggatg gcggccggtg gaatctacga ccagatcggt ggagggttcc accgctactc
     1561 gaccgatcgc gtctggctgg ttccccactt cgagaagatg ctgtacgaca acgcgcagct
     1621 cgcgaccctc tacctccatg cgtggcaggt gagtgggaac gaccgctacc gcgaggtcgt
     1681 cagcgagacg ctcgagtacc tcctacgcga gatgcggcac gaggacggcg gcttcttctc
     1741 gtcgcaggac gctgactcgg agggcgtcga ggggaagttc tacgtctgtc ctgggacgag
     1801 ctcgtcgacg tcgtcggcga aaagaaggcg cgcgacctcg gcgggtcgcc ggaaggcaac
     1861 tgggaaggca ccaacatcct ctcgagcgac gcggcggcgt ccgttccggc ggacgtccgc
     1921 gctcgtctgc tggacgtgcg gagccggcgc gtgtggcccg gaaccgacga caaggtcctc
     1981 gtggcgtgga acgcgctcgc gatcgcggcg ttcgccgagg ccgggcgcgt gctgcgtgag
     2041 gagcgatgga tcgcggccgc cgagcgggcc gccgacttcg tcctcgcgag catgcgacga
     2101 cgcgacgacg gccgcctgct tcgggcgtgg cgcgatgggc gaacgagcgg cgccgcgtac
     2161 gccgacgact acgcgctgat ggcagcggca tgcctgacgc tgtacgagac gacgttcgac
     2221 acgcggtggt tcacggaagc gcgcgcgctc gcggacgacc tccttcggct gttccatgac
     2281 ggcgacggcg gcgggttctt ccagaccggg atcgacgccg aacggctcgt gctgcgcccg
     2341 aaggagctgt tcgacaatgc ggtgccatcg gggaactccg cggcagcgga ggtgctcctg
     2401 cgcctcgcgt tcctcaccgg cgagggaagc tacgagtcgg cggcggtgtc ggcgctccgc
     2461 ctcgtccgcg actatctcga gagggccccg acgggcttcg gcaacgcgct ggccgcgctc
     2521 gaccgctacg tcggcccgtc gaaggagatc gcgatcgtcg gcgacctcgc gagcgacgaa
     2581 gcgggggacc tcgtagagac cgtgtggcgc gagtaccggc cgaagatcgt catggccgcg
     2641 ggatcaccgg accgcggcga cgtcgtcccg ctcctagccg accgcacgac ggtcgacggc
     2701 gggcccgcgg cgtacgtctg cgagcggttc gcctgccgga tgccggtatc ggatccagag
     2761 gccctcacgg cgcagctcga ctagggccca gtggcgagct cggtcctcag ctcaccgcct
     2821 tcttcacgag cgcggcgatc cttgcctctt cggcggcggt cagctccttc agcgcgaagg
     2881 aggtcggcca catggcgcct tcgtcgaggt tcgccgcggc gttgaagccg aacgtcgcgt
     2941 acctctcctt gaacttcgac gcagctgtga agtagcagac gaccttgccc tcctggttgg
     3001 cgtacgcggg catcccgtac caggttctcg gcgagagggc aggcacggtg gctttgacga
     3061 tcgcgtggag ccgctccgcc atggcgcgat ccggttgcgt catctcggcg atcttcgcga
     3121 gcacgtcgcg ttccccgtcc gccttgcccg cgccccggcc gcggcgggct tccgccttca
     3181 gctcttgggc gcgctccttc atcgcgactc gttcctcgtc agtgaatccc ttcgacgtct
     3241 tccccttcgc ggtggtgcgc ttggcgggcg tctgcgtggc ctttcttgca gccttcttct
     3301 caggcatttc gaagcacctc cgtgcgcaga acgaggccca gccgcttgac cgccggggat
     3361 gataaccgtc gtccctgtct ctgtggcggg ccaactcgtc ccacgtaggt cagacgcgcc
     3421 gttccccctc cacgccctca gctccgatga tccggttcag cgagctccgg agctgcttca
     3481 cgccgcggct gccgagaagg ccttcgagat ctcgctcgat cacggcgatc gtcccgaacg
     3541 ccgcctcgac gcaggcgatg cccctccggg tcagcacgat gcgcttcgcg cggcggtcct
     3601 cgggatcggg ccggcgctcg acgtaccgca tgcgctcgag gtcgtcgacg agctccccca
     3661 tggcttgcgg cgtgatgttc gctcgcccgg cgagctcggt caggcgagtg cctccctcga
     3721 cgtcgatgtg cgcgaacacg gaagagtgag cggggcgctg cgggaacccc gcggccgcca
     3781 caccggcgac gatcctgtgt cccagcgcgg agtacgcctc tccgaggagg gccgccgtgt
     3841 tgacggg
//
LOCUS       DASZOB010000114         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132474, whole genome shotgun sequence.
ACCESSION   DASZOB010000114 DASZOB010000000
VERSION     DASZOB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132474"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VG709_01815"
     CDS             complement(<1..824)
                     /locus_tag="VG709_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC31848.1"
                     /translation="MSGAPLPAAPGAPSDIGPPVEIERRNARTAIETLLRGRIRGRRT
                     AGKDLVATVALDLGDAGEWTLKIDRGRMSLAPDRVRRPDTTIQTDAVTLADIVSGSRA
                     GVEAFLSGDLRVRGNLALSLRLDDLFGRPDRPARYPRARTVRAGGLSTFILEAGKGPP
                     VVLLHGLGATNASLLPTLWDLAVDHRVIAPDLPGFGESGKPIRRYDAPFYARWLLAFL
                     DELGIERAHLVGNSMGGRVAIETALLAQHRVDRMVLLAPSPAFIRRREFVRVVRLLR"
     gene            complement(898..>2226)
                     /locus_tag="VG709_01820"
     CDS             complement(898..>2226)
                     /locus_tag="VG709_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC31849.1"
                     /translation="VGAGRAGLSAATDIAAAGGSVVVLEARDRVGGRTLTEELDGGHL
                     VELGGQWIGPTQDRMYALVRDLGIETYPTYDAGDQVALLAGKRYRFSGKLPRLNPLVV
                     ADFAQGFYRLERMAKRIDPERPWRAERARDWDGQTVETWVRRHMRTPLARETMRLFVR
                     ALLTTEPSSLSLLFTLFYFRSGTSFENLVSTTGGAQQDRLVGGAHLVSTRLAERLGDR
                     VVLRAPVRAIEHGHDVRVATDERTWSARRAIVAVPPALAATIAYAPALPGHRAQLTQR
                     MPHGATIKSVAVYERPFWRDEGLSGQAGGLDLPVEFTFDTSPRDGYPGMLVGFVEGDR
                     ALVLGRMSADARRKLVLDSFARYFGPKAAEPIAYADRDWTEEEWTRGCFGGHMPPGAL
                     TRFGPALREPVGPIHWAGTESARVWAGYMEGAVESGRRAASEVLEALSRG"
BASE COUNT          321 a          837 c          783 g          285 t
ORIGIN      
        1 cgcagcaggc ggacgacgcg cacgaactcg cgccttcgga tgaacgccgg cgacggcgcg
       61 aggagcacca tgcggtcgac gcggtgctgc gcgagcagcg ccgtctcgat cgccacgcgt
      121 ccgcccatcg agttaccgac gaggtgcgcc cgctcgatcc cgagctcgtc gaggaacgcg
      181 aggagccagc gcgcgtagaa gggcgcgtcg taccgccgga tcggcttgcc gctctccccg
      241 aagcccggga ggtccggcgc gatcacgcgg tggtcgaccg ccaggtccca cagcgtggga
      301 agcaacgacg cgttcgtcgc accgagccca tgcagcagca cgacgggtgg acccttcccg
      361 gcctcgagga tgaacgtgct caggccaccc gcgcgcaccg tccgcgcgcg cgggtagcgt
      421 gccgggcggt cggggcgccc gaacaggtcg tcgagccgga gcgagagcgc gaggttgccg
      481 cgaacacgaa gatcccctga caggaacgcc tcgacgcccg cccgcgaccc cgacacgatg
      541 tccgccagcg tcaccgcgtc ggtctggatc gtcgtgtccg gccgacggac gcggtcgggg
      601 gcgagcgaca tccggccgcg atcgatcttg agcgtccact cgccggcgtc gcccaggtcg
      661 agcgcgacag tggcgacgag gtccttgccg gcggtacgcc gaccgcggat acgcccgcgg
      721 agcaacgtct cgatcgccgt acgagcgttc cgccgctcga tctcgacggg cggcccgatg
      781 tccgacggtg cgcccggcgc ggcgggcaac ggcgctccgc tcatgcgtcg tcctccctta
      841 cccactcccg accgattcga gcatgccagg ccggtccgcc gttcgcgcgg gttcggttca
      901 cccgcgcgag agcgcctcga gaacctccga ggccgctcgg cgccccgatt ccacggcccc
      961 ctccatgtac cccgcccaga ctcgggcgct ctccgtgccc gcccagtgga tcggcccgac
     1021 cggctctcgg agcgcgggac cgaaccgcgt gagcgcgccc ggaggcatgt gccccccgaa
     1081 gcagccgcgc gtccattcct cttcggtcca gtcccggtcc gcgtacgcga tcggctccgc
     1141 ggccttcggc ccgaagtacc gcgcgaacga atcgaggacg agcttcctgc gcgcatccgc
     1201 cgacatgcga cccagcacga gggcgcggtc cccctcgacg aacccgacga gcatcccggg
     1261 gtacccgtcc cgaggcgacg tgtcgaacgt gaactcgacc gggaggtcga ggcccccggc
     1321 ctgcccgctc agcccctcgt cgcgccagaa cggccgctcg tagaccgcca cggacttgat
     1381 cgtggcaccg tgcggcatcc gctgcgtgag ctgcgcgcgg tggcctggga gcgccggggc
     1441 gtacgcgatc gtcgcggcga gggcgggcgg caccgcaacg atcgcgcggc gcgcgctcca
     1501 cgtccgctcg tcggtcgcca cgcggacgtc gtgcccgtgc tcgatcgcgc gcaccggcgc
     1561 acggaggacc acgcgatcgc cgagccgttc ggcgaggcgc gtcgagacga ggtgcgcgcc
     1621 gcccaccagc cggtcctgct gcgcacctcc cgtcgtgctg acgaggttct cgaagctcgt
     1681 gcccgaacgg aagtagaaca gcgtgaacag cagcgagagc gacgacggct cggtcgtgag
     1741 cagcgcgcga acgaacaggc gcatggtctc gcgtgcgagc ggagtccgca tgtgacggcg
     1801 gacccaggtc tcgacggtct gtccgtccca gtccctcgcg cgttccgcgc gccacgggcg
     1861 ctcggggtcg atgcgcttgg ccatccgctc gaggcggtag aagccctgtg cgaaatccgc
     1921 gacgacgagg gggttgaggc gcggcagctt cccggagaac cggtaccgct tgcctgcgag
     1981 gagcgccacc tggtcgccgg cgtcgtacgt cggatacgtc tcgatgccga ggtcgcgcac
     2041 gagcgcgtac atgcggtcct gcgtcgggcc gatccattga ccgcccagct cgacgagatg
     2101 ccctccgtcg agctcctcag tgagcgtccg tccgccgacg cggtcccgtg cctcgaggac
     2161 gacgacggag ccgccggccg ccgcgatgtc ggtcgcggcc gagagcccgg cgcgccccgc
     2221 gccgac
//
LOCUS       DASZOB010000115         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_153832, whole genome shotgun sequence.
ACCESSION   DASZOB010000115 DASZOB010000000
VERSION     DASZOB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153832"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VG709_01825"
     CDS             <1..195
                     /locus_tag="VG709_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HWC31850.1"
                     /translation="EFDEELVELYEGLRAQKRGVGAASLEDGVCQGCHQRLSALELDR
                     LKRTEGIRRCEYCRRILVMR"
     gene            192..647
                     /locus_tag="VG709_01830"
     CDS             192..647
                     /locus_tag="VG709_01830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HWC31851.1"
                     /translation="MIVHTDGAARGNPGPAGIGVVVRRSDGEVVAELAEGIGPATNNV
                     AEYRAAIEGLRLAARHGARRVTLRAESRLLIEQRAGRFRVKNPTLQRLHEEARREIKR
                     FDDVRLEHVPRERNADADRLANVAVDDWLAGPGRDYMPDEPAPPLFEGD"
     gene            complement(602..1522)
                     /locus_tag="VG709_01835"
     CDS             complement(602..1522)
                     /locus_tag="VG709_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31852.1"
                     /translation="MRLAACAALVVCLGCSGGPVAPARSEAFDADAEQPAGDFTAIGL
                     EAAAALETARAFLEAYERPRTLAATHRRLVDGIDLSEWVYWVGVQNAGLDFVRGGGVE
                     IDGLRISQLRLPFATVAVDAAALTRFRTDGAVGARERRFAALLLQRVESGWRVVDALH
                     DARQMTSTITVFDPPIEVTSDSVDVEVRSVFRFTGGTVVNVSVANRRGVPLEVDLERS
                     LLVVDDEPVRGEQMTRSLMSPVEPGGDAEGGITFPTIPVEAIPQALSLTMRGFGSVNV
                     LAELPAEPFLLDSPAGQSPSNRGGAGSSGM"
     gene            1564..1946
                     /gene="rnpB"
                     /locus_tag="VG709_01840"
     ncRNA           1564..1946
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VG709_01840"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          325 a          744 c          771 g          277 t
ORIGIN      
        1 gagttcgacg aggagctcgt cgagctgtac gaggggctcc gcgcgcagaa gcgtggcgtc
       61 ggcgcggcgt cgctcgagga cggcgtgtgc cagggctgcc atcagcggct ctctgcgctc
      121 gagctcgacc ggctcaagcg cacggaaggc atccggaggt gtgagtactg ccgtcggatc
      181 ctcgtgatgc ggtgatcgtc cacaccgacg gggcggccag agggaatccc ggcccggcgg
      241 gcatcggcgt cgtcgtccgt cggtcggacg gcgaggtcgt cgcggagctt gcggagggca
      301 tcggtcccgc gacgaacaac gttgcggagt accgggccgc gatcgagggg ctccggctcg
      361 ctgcccgcca cggcgcgagg cgcgtgacgc tccgagccga gagccgcctg ctcatcgagc
      421 agcgggccgg gcgcttccgg gtgaagaacc ccacgctgca gcggctccac gaggaggccc
      481 ggcgcgagat caagcggttc gacgatgtgc gtctcgaaca cgtgcctcgc gagcgcaacg
      541 ccgacgccga ccgcctcgcg aacgtcgcgg tcgacgactg gctcgccgga ccggggcgcg
      601 actacatgcc cgacgagccg gccccgcccc tgttcgaggg cgactgaccg gcgggcgagt
      661 cgagaaggaa cggctccgcc ggaagctccg ccagcacgtt gacggacccg aagccccgca
      721 tcgtgagcga cagcgcctgc gggatggcct cgaccgggat cgtagggaac gtgatgccgc
      781 cttccgcgtc gccgccgggc tcgaccggcg acatcagcga tctcgtcatc tgctcgcccc
      841 tgactggctc gtcgtcgacg accagcagcg atcgctcgag atcgacctcg agcggcacgc
      901 ctcggcggtt cgcgacgctg acgttcacga cggttccgcc ggtgaacctg aagacgctcc
      961 ggacctcgac gtcgacgctg tccgacgtga cctcgatcgg ggggtcgaag accgtgatcg
     1021 tgctcgtcat ctgccgggcg tcgtgcagcg cgtccacgac cctccatccc gactcgacgc
     1081 gctggaggag aagcgcggcg aagcggcgct cgcgcgcgcc gaccgcgccg tccgtccgga
     1141 agcgcgtcaa cgcggcggcg tcgaccgcga cggtggcgaa agggagccgg agctgcgaga
     1201 tgcggagccc gtcgatctcg acgccacctc cacggacgaa gtcgagaccc gcgttctgga
     1261 cgcccaccca gtacacccat tcgctcaggt cgatcccatc gacgagacga cggtgcgtcg
     1321 ccgcgagcgt ccgcggacgt tcgtaggcct cgaggaacgc gcgcgccgtc tcgagcgccg
     1381 cagcggcctc gagcccgatc gcggtgaagt cgccagcggg ctgctctgcg tccgcgtcga
     1441 acgcctccga gcgggcgggc gcgaccggac ctccggaaca cccgaggcag acgacgagcg
     1501 cggcgcacgc ggcgagtcgc acgttccggg cgaggatagc cccggcccgt atacttgacg
     1561 tgaggagtcg gccgggcggc cgcggcggaa acgtcgagga aagtccggac tccgcagggc
     1621 agggtgctgg gtaacgccca ggcggggtga cccgcggaaa gtgccacaga gagcagaccg
     1681 ccgatggccc ctcggggcac aggtgagggt gaaagggtga ggtaagagct caccagcgcg
     1741 gcgggcgacc gtcgcggctc ggcaaacccc atccggagca aggccaagca gggagcttcc
     1801 ggccgaggcg atcgcacggc cggagcgggt tgcccgcccg atgctcccgg gtaggccgca
     1861 tcgaggtccg cggcgacgcg ggccccagag agatggtcgc caccccttcg gggcaacaga
     1921 atccggctta caggccggct cctccacctc ctctgctagc gttggccggc gtggccctct
     1981 cgtcccgttc gaagctcctt ctgatggcgc tcggcctggt ggccgcggtc gccgtcatct
     2041 cgctcggcgc cgcgttccgg ccgtccgacg accccggctc cgtcatcgag ctgtccgggc
     2101 cgatgccctc gctggag
//
LOCUS       DASZOB010000116         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_103517, whole genome shotgun sequence.
ACCESSION   DASZOB010000116 DASZOB010000000
VERSION     DASZOB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103517"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VG709_01845"
     CDS             complement(<1..567)
                     /locus_tag="VG709_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC31853.1"
                     /translation="MSERTCRRCGAPLASDARFCSNCGMPVETAAAEERKLVSVLFAD
                     VAGSTELAARLDAERFREVMGAVYRMASDEVASLRGRTEKFIGDAIMAVFGLPHAHED
                     DALRAVRAGLRIRDRVEHLGEELALGSPLRVRVGVNTGPVVASTATSGEFLVSGPAVN
                     AAARLEQAATPGEVLVGDMTWQLTRDSAE"
     gene            676..891
                     /locus_tag="VG709_01850"
     CDS             676..891
                     /locus_tag="VG709_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31854.1"
                     /translation="MERYHVVPVDDLVGHEETGDECVCGPHVEFYLKGQVVVHHALDG
                     RNASDCGWARRRASEVARECELTDELA"
     gene            complement(949..1725)
                     /locus_tag="VG709_01855"
     CDS             complement(949..1725)
                     /locus_tag="VG709_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HWC31855.1"
                     /translation="MDRTREVRETTTYGTGGVAEARARYGGLDIPATIGGFLAALGAL
                     AILAAIAGAILGSIGYQTGLPDLPGTQQEISMGAAIAGFVVLFLGFLIGGWVAGRIAR
                     YDGGRNGIMVAIWAIILVGVMTALGAVLGDEFNITQRVNLPNWFTSEAFTTGALISGL
                     IALATMLIASWLGGLWGSSYHRRADAVVAGDGYATTPVHQETTTARDDVVTREQADGV
                     QQPAYADRDRDGRPDVQEQATATQQPVYPDRDRDGRPDTG"
     gene            1821..>2208
                     /locus_tag="VG709_01860"
     CDS             1821..>2208
                     /locus_tag="VG709_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31856.1"
                     /translation="MRHSPAALAVLAAFSVLVPVHAAPQSRAVEQETWEPVYLLQVTV
                     RSTAGPAAVRLDEPAVMIEGVALHRARGTRASFGEFGPGPLRVRPRARRDRASATFVV
                     AVTPGGAKSFRFVVRAPRRGTRVPVRN"
BASE COUNT          293 a          813 c          775 g          327 t
ORIGIN      
        1 ctctgcggaa tcgcgcgtga gctgccaggt catgtccccg accaggacct cccccggagt
       61 ggccgcctgc tcgagccgtg cggcggcgtt cacggccggg ccggagacga ggaactcgcc
      121 cgacgtcgcc gtgctcgcca cgaccggccc ggtgttgacg cccacccgga cgcggagcgg
      181 cgagccgagc gcgagctctt ccccgagatg ctccacacgg tcgcggatcc gaagccccgc
      241 acggaccgcg cggagcgcgt cgtcttcgtg cgcgtgggga agcccgaaca cggccatgat
      301 ggcatcgccg atgaacttct cggtgcggcc gcgcagcgag gccacctcgt cggacgccat
      361 gcggtagacc gcgcccatca cctcgcggaa tcgttcggcg tccagcctgg ccgcgagctc
      421 ggtggagccg gcgacgtccg cgaacaggac ggacacgagc ttccgctctt cggccgcggc
      481 ggtctcgacg ggcatgccgc agttcgagca gaaccgggca tctgaagcca gcggggcgcc
      541 gcaccgccgg caggtgcgct cgctcacggc tccaaggata cgggagcctc cgccggcggc
      601 cgtcgcggaa attcctccga tgatgttctg ccgcgtccct caggcggcgc ggggggcgtg
      661 ccgatggtcc ggggcatgga gcgctaccac gtggtgccgg tggacgacct ggtcggccac
      721 gaggagaccg gcgacgagtg cgtctgcggt ccgcacgtcg agttctacct caagggccag
      781 gtggtcgtgc accacgccct cgacggacgc aacgcctcgg actgcggatg ggcgaggcgc
      841 cgggcgtccg aggtggcgcg cgagtgcgag ctcaccgacg agctggccta ggccccaacg
      901 cgaagagggc gcctctcggc gccccctccg gtcgtcgtgg ggtcgtccct aacccgtgtc
      961 cgggcgaccg tcccgatccc gatccgggta caccggctgc tgcgtcgccg tcgcctgctc
     1021 ctgcacgtcc ggccgaccgt cgcgatcgcg gtccgcgtac gccggctgct gcaccccgtc
     1081 cgcctgctcc ctggtgacca cgtcgtcacg cgccgtggtc gtctcctggt ggacgggggt
     1141 cgtcgcgtat ccgtcaccag cgacgaccgc gtcggcgcgg cggtggtagc tcgatcccca
     1201 cagcccgccg agccacgatg cgatcagcat cgtcgcgagc gcgatcaggc cgctgatgag
     1261 cgcccccgtc gtgaacgcct cgctcgtgaa ccagttcggg aggttcacgc gctgcgtgat
     1321 gttgaactcg tcgccaagga ccgcgccgag cgccgtcatg acgcccacga ggatgatcgc
     1381 ccagatcgcg accatgatcc cgttgcgccc gccgtcgtag cgggcgatcc gaccggcaac
     1441 ccagccgccg atcaggaacc cgaggaagag cacgacgaag ccggcgatgg cggcgcccat
     1501 ggagatctcc tgctgggtcc ccggcaggtc cggcaggccg gtctggtagc cgatggatcc
     1561 gaggatggcc ccggcgatcg ccgcgaggat cgcgagcgcg ccgagcgcgg ccaagaaccc
     1621 accgatcgtc gccgggatgt cgaggccgcc gtaccgggct cgcgcctccg cgacccctcc
     1681 cgtgccgtac gtggtggtct cgcggacttc tcgtgtcctg tccacgtcga tccccccttc
     1741 cgtagagaga acgttcatct aactgttacc caccccgtgt gctgcggaaa cgcggccggg
     1801 gtccgtcgta cgatgctcgc gtgcggcact ctcccgctgc tctcgctgtc ctcgcggcgt
     1861 tcagcgtcct cgtaccggtc cacgcggcgc cccagtcgcg ggccgtcgag caggagacgt
     1921 gggagccggt ctacctgctg caggtgaccg tccgctccac ggcggggccg gcggcggttc
     1981 gtctggacga acccgcggtg atgatcgagg gcgtcgcgct gcaccgggca cgagggaccc
     2041 gcgcgtcgtt cggtgagttc ggtcccggtc ccctccgcgt tcgaccgcgt gcgcgtcgcg
     2101 accgggcgtc cgcgacgttc gtcgtcgccg tcacccccgg cggcgcgaag tcgttccgct
     2161 tcgtcgtgcg ggccccgcgc cgcggtacac gcgtgccggt tcggaacg
//
LOCUS       DASZOB010000117         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_163954, whole genome shotgun sequence.
ACCESSION   DASZOB010000117 DASZOB010000000
VERSION     DASZOB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163954"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VG709_01865"
     CDS             <1..497
                     /locus_tag="VG709_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31857.1"
                     /translation="TLVQRWDDLDEGKRREAVELIAFDAERGAAMTELVVAISRAASG
                     RQRRPERGRLEDAGASVSAAFARIDERPDVLWEATGDVAVDQRTLHAIAFVLVDVASV
                     ERKAPLAVASEISPDAVSVSIARDGASSADGGAMAIVRRAAAAVGGGLEAHRDGRLVL
                     RLPR"
     gene            complement(499..972)
                     /locus_tag="VG709_01870"
     CDS             complement(499..972)
                     /locus_tag="VG709_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWC31858.1"
                     /translation="MTTPVAASSGRSSTIVTSISIERPPDLVWPYLVDWERLGTWMQE
                     ASDIQVPSAVREGVGVEAVATIRIAGITTRDPIHVTRWEPPRVLEIRHLGWVKGTGYM
                     QLSPSATGTDVHWREEYEPPWGPLGAAGMRMVRPLMRRVFRRDLRALKEKVERER"
     gene            complement(969..1502)
                     /locus_tag="VG709_01875"
     CDS             complement(969..1502)
                     /locus_tag="VG709_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31859.1"
                     /translation="MFDSERTTDVQSIPVRFPGRRLAVLLATAALLVACGEEDGRDPR
                     ARNRTGDQPPGRLAGGEATLKVSGDLDADVRLALRPESVYSAPPGGMALSYDDEAGNF
                     LGIGGETPSGTRPTSLQLSLTITVDGEAAFESKGGECTIDVDRADASGVDGSFECNDL
                     ANETASIDAVGAFTAFA"
     gene            1524..>1821
                     /locus_tag="VG709_01880"
     CDS             1524..>1821
                     /locus_tag="VG709_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWC31860.1"
                     /translation="MEPAEALRILEDARASGLAAIARAGTLDELRDAEVAVLGRKAPL
                     SHVQRSLGAVPAESRRDVGRATNEVREALRAALEERERELRADAERRVAEADRID"
BASE COUNT          245 a          666 c          660 g          250 t
ORIGIN      
        1 cgacgctcgt ccagagatgg gacgacctcg acgaagggaa gcgccgagaa gccgtcgagc
       61 tgatcgcctt cgacgccgag cgtggcgccg cgatgaccga gctcgtcgtc gcgatctcgc
      121 gtgcggcgtc ggggcggcag cgccgtcccg agcgcggacg gctcgaggac gcgggcgcct
      181 cggtctcggc cgcgttcgcg cggatcgacg agcgtcccga cgtcttgtgg gaggcgaccg
      241 gcgatgtcgc ggtcgaccag cggacgctcc acgcgatcgc gttcgtgctc gtggacgtcg
      301 cgtccgtgga acgaaaggcg ccgctggccg tcgcgtcgga gatctccccc gatgccgtct
      361 ccgtttcgat cgcacgcgac ggagcttcgt cggcggatgg cggagcgatg gcgatcgttc
      421 ggcgcgccgc cgccgcggtc ggcgggggcc tcgaggcgca ccgtgacggc cggctcgtcc
      481 tgcggctccc gcgatagatc agcgctcccg ttcgaccttc tccttgagcg cccggaggtc
      541 gcggcggaac acccgccgca tgagcggccg gaccatccgc atcccggcgg cgccgagcgg
      601 gccccacggc ggctcgtact cctcccgcca gtggacgtcc gtgcccgtcg cgctcgggga
      661 tagctgcatg tagccggttc ccttgaccca cccgagatgc ctgatctcga gcacgcgcgg
      721 cggctcccaa cgggtgacgt ggatcgggtc gcgcgtcgtg atgccggcga tgcggatcgt
      781 cgccacggcc tctacgccga cgccctcgcg gacggcgctc ggcacctgga tgtcgctggc
      841 ctcctgcatc cacgtgccga gccgttccca gtcgacgagg tacggccaga cgaggtcggg
      901 cgggcgctcg atcgagatcg aggtgacgat cgtcgacgaa cgtcccgacg aggcggcgac
      961 aggcgtcgtc acgcgaaggc cgtgaaggca ccgacggcgt cgatgctcgc ggtctcgttc
     1021 gcgaggtcgt tgcactcgaa cgagccatcg accccgctgg cgtcggcgcg gtcgacgtcg
     1081 atcgtgcact cgccgccctt cgactcgaac gccgcttcgc cgtccaccgt gatcgtgagc
     1141 gacagctgca gcgacgtcgg acgggttccg ctcggcgtct cgccgccgat cccgaggaag
     1201 ttgcccgcct cgtcgtcgta ggagagcgcc ataccgccgg gcggagcgga gtagaccgac
     1261 tcgggacgga gcgcgagcct cacgtccgcg tcgaggtcgc cgctcacctt gagggtcgcc
     1321 tcgccgccgg ccaggcgacc gggaggctgg tcgccggtcc ggttacgcgc gcgtgggtcg
     1381 cggccgtcct cctcgccgca cgccacgagc agcgccgccg tcgcgagcag cacagcgagc
     1441 cgtcggcctg ggaagcgcac ggggatcgat tgaacgtcgg tcgtccgctc ggagtcaaac
     1501 accgtcgcta cacttaccgt cccatggagc cggccgaagc cctccgcatc ctcgaggacg
     1561 cacgcgcgag cggcctcgcc gccatcgcgc gagccgggac gctcgacgag ctccgggacg
     1621 ccgaggtggc cgtgctcggc cgcaaggcgc ccctcagcca cgtccagcgg tcgctcggcg
     1681 ccgtgcccgc cgagtcgcgc cgcgacgtcg ggcgcgccac gaacgaggtc cgcgaggcgc
     1741 tccgtgccgc gctcgaggag cgagagcgcg agctgcgcgc ggacgccgaa cgtcgcgtcg
     1801 ccgaagccga ccgcatcgac g
//
LOCUS       DASZOB010000118         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_73050, whole genome shotgun sequence.
ACCESSION   DASZOB010000118 DASZOB010000000
VERSION     DASZOB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73050"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VG709_01885"
     CDS             <1..281
                     /locus_tag="VG709_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31861.1"
                     /translation="VSCAFKAAGRFRQSGDVSMELLARGLLLALIGLLVADFFVSEQY
                     SKQLWLLLGLAPALLALARRPPEEESPAGGSSSGSVSSIDVPVVPAAR"
     gene            332..1447
                     /locus_tag="VG709_01890"
     CDS             332..1447
                     /locus_tag="VG709_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWC31862.1"
                     /translation="MLVLVDAIGARVAGAESFAVGLAAALPRERFKVTVCASREVIGD
                     LPDALVDSDLGFLCLERGRDGLKPHAFVRLARRLRRDRIDVLHAHKFGSNVWGTLLGR
                     LCRVPVIVAHEHTWSFEGEPVRRLLDRHLVGRLASVFVAVSSVDAERMTSVEKVPPEK
                     VVVLPTAFIPRPPGPHSDVRTELGLRASEPLLVTAAFLRPQKALDVLIDAFALALNKV
                     PAAHLVIAGTGSCEADLRRHAVASGVADRVHFLGVRNDVGAILETGDVAVMSSDYEGL
                     PLFALECMAHRTPLVSTDVGGIRDILEEDESVLLAPPRDPHALASQIERVLLEPATRE
                     KLVAGAQERIAGLTIDQVATRFANLYDRHLEHARGPR"
BASE COUNT          212 a          526 c          526 g          265 t
ORIGIN      
        1 cggtgtcgtg cgcgttcaag gccgccggtc gcttccgaca atccggagat gtctcgatgg
       61 agctcctggc acgtggcctg ttgctcgcgt tgatcggcct gctggtcgcg gatttcttcg
      121 tgtccgagca gtacagcaaa cagctctggc ttttgctcgg actggctccg gcgttgctcg
      181 cgctcgcacg tcgtccgccg gaggaggagt cgccggctgg aggttcgagc tccggaagcg
      241 tgagctcgat cgacgtgccg gtggtgcccg ccgcgaggta gccttggcga gggcgcggca
      301 cgacctggct tccgaagctc ggcgcacccg cgtgttggtg ctcgtggacg cgatcggcgc
      361 gagggtcgcc ggggcggaga gcttcgccgt cggccttgcc gcggctttgc cgcgcgagcg
      421 gttcaaggtg accgtctgcg cttcgcgcga ggtcattggg gacctccccg acgcgctggt
      481 cgactcggac ctgggcttcc tctgcctgga gcggggccgc gacggcctca agccgcacgc
      541 gttcgttcgg ctggcccggc gcctgcggcg tgaccgaatc gacgtgctgc acgcgcacaa
      601 gttcggatcc aacgtgtggg gaacgctact cggccgtctc tgccgcgtcc cggtgatcgt
      661 tgcgcacgag cacacctggt cgttcgaggg cgagccggtc cgccgcctgc tcgatcgcca
      721 cctcgtcggc cggttagcgt ccgtcttcgt tgccgtctcg agcgtcgacg cggagcggat
      781 gacgtccgtc gagaaagtgc cgcccgagaa ggtcgtcgtc ctaccgaccg cgttcattcc
      841 ccgacctccg ggtccgcaca gcgacgtgcg taccgagctc ggcctccgag cgagtgaacc
      901 gcttctggtc accgcggcct tcctgagacc ccagaaggcg ctcgacgtcc tgatcgacgc
      961 gttcgccctc gcgctcaaca aggtgccggc ggctcacctc gtcatcgcgg gcacaggatc
     1021 ttgcgaagcc gacctgagac gccacgcagt cgcctcgggc gtcgccgacc gcgtacattt
     1081 ccttggcgtc aggaacgacg tgggcgccat cctcgagacg ggcgacgtgg cggtgatgag
     1141 ctccgactac gaaggattgc cgctgttcgc tctcgagtgc atggcccatc gcacgccgct
     1201 cgtgtcaacc gacgtcggcg gcatccggga catcctcgag gaggacgaga gcgtcctgct
     1261 cgctccaccc cgggatccgc acgccctcgc gagccagatc gagcgggtct tgctcgaacc
     1321 cgcgacgcgc gagaagctcg tcgcgggagc tcaggagcgg atcgccggtc tgacgatcga
     1381 ccaggtggcg actcgcttcg cgaacctcta cgatcgccac ctcgagcacg cgcggggccc
     1441 ccggtgatcc tccgcgatga cgacggccgc cgggtgtgtc gggtagacag cacaagcgcg
     1501 tcgacccagc cgtaggctca gggcagcga
//
LOCUS       DASZOB010000119         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_112543, whole genome shotgun sequence.
ACCESSION   DASZOB010000119 DASZOB010000000
VERSION     DASZOB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112543"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VG709_01895"
     CDS             <1..820
                     /locus_tag="VG709_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31863.1"
                     /translation="VWPGDRDALARALERIRRPSGADGTKSGRAVAVFGARGGAGVTF
                     LATNLAAAFAARGATCVLADLDPFYTDVSVALGVSPNGGAPTIADLAPVLDELTVEHV
                     ERVLYRHPRGFHTLLAPAVPGAVRLSPDHVGAVARTLRAAADVAILHLPRSLDDTTVA
                     AMEAADDVLLVVTLDVVAFRDARRTLERLEAAGVDGRCKLVINRATRAEVAPDDAEHV
                     FGIRPVSVIRADRSVGRAQNRGEVLAGRSGAAARRVAALAERLLDHAAAEGGSA"
     gene            817..1992
                     /locus_tag="VG709_01900"
     CDS             817..1992
                     /locus_tag="VG709_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWC31864.1"
                     /translation="MTVRWHPAGGQARLKALVAERVRERTDPAELSRLTPAERRLRVS
                     DTAMSVLREHRVVLSARDVTRLVNQISDEVVGFGPIEALLKDPDVTEVMVNGADDVYV
                     ERKGRLEKVERGLFEGEEAVYHLIERIVGPLGLRVDEACPYVDARLPDGSRFHAIVPP
                     LSLCGPVLTLRKFGVAPLGPQDLVRVGTAPDEVMSFLAESVRARANIVISGGAASGKT
                     TMLGVLSSFIPPGERIITIEDAAELRLAQPHVVRLEARPANVEGKGAVTVRDLVRNAL
                     RMRPDRLVVGEVRGGEALDMLQAMNTGHEGSMSTAHANSPHELMSRLETMALMSDVDL
                     PALHVREQIGSTIDLVVHLARFPDGRRIVSRLARVCGYRDGRVVLEDALPEDRWEVA"
BASE COUNT          277 a          745 c          779 g          310 t
ORIGIN      
        1 cgtgtggccg ggggaccgcg atgcgctcgc gcgggcgctc gagcgcatcc ggcggccgag
       61 cggagccgac gggacgaaga gcggacgcgc cgtcgccgtt ttcggcgctc gcggcggtgc
      121 cggcgtgacg ttcctcgcga cgaatctcgc tgccgcattc gccgcccgcg gcgcgacgtg
      181 cgttctcgcc gacctcgatc cgttctacac ggacgtctcg gtagcgctcg gcgtctcgcc
      241 gaacggtgga gcgccgacga tcgcggatct cgcccccgtg ctcgacgagc tgacggtcga
      301 gcacgtcgag cgggtgctgt accgccatcc ccgtggtttc cacacgctgc tcgcgccggc
      361 ggtccccggc gcggtgaggc tgtcgccgga ccacgtcggg gcggtcgcga gaacgctccg
      421 agcggccgcc gacgtcgcga tcctccatct cccgcggtca ctcgacgaca cgacggtcgc
      481 cgcgatggag gccgccgacg acgtgctgct ggtcgtgacg ctcgacgtcg tcgcgttccg
      541 cgacgcgcgg cgcacgctcg agcgcctcga ggcggcgggc gtcgacggac gctgcaagct
      601 cgtcatcaac cgagcgacgc gcgccgaggt tgcgccggac gacgcggagc acgtcttcgg
      661 gatccggccg gtctcggtca tccgcgccga tcgctccgtg ggacgcgccc agaaccgagg
      721 tgaagtactc gccgggcgtt ccggtgccgc ggcacggcgg gtcgccgccc tcgccgagcg
      781 cctcctcgac cacgcggcag cggagggagg aagcgcgtga ccgtccggtg gcatcccgcc
      841 ggtggacaag cacggctcaa ggcgctcgtc gccgaacgcg tccgggagcg taccgatccc
      901 gcagagctct cgcggctcac gccggcggag cggcggctcc gagtcagcga caccgcgatg
      961 agcgtgctgc gcgagcaccg cgtcgtgctg tcagctcgcg acgtcacgcg gctcgtcaac
     1021 cagatctccg acgaggtcgt cggcttcgga cccatcgagg ccctcctcaa ggaccccgac
     1081 gtcaccgagg tgatggtgaa tggcgccgac gacgtctacg tggagcggaa ggggcggctc
     1141 gagaaggtgg agcgcggtct attcgagggg gaggaggccg tgtaccacct gatcgaacgc
     1201 atcgttggtc cgttgggcct ccgggtggac gaggcgtgtc cgtatgtcga cgctcgactc
     1261 ccggacggct cgaggttcca cgcgatcgtg ccgccccttt cgctctgcgg cccggtgctc
     1321 acgctccgca agttcggggt cgcgccgctc gggccgcaag acctcgtccg cgtcgggacc
     1381 gcgcccgacg aagtcatgtc gttcctcgcc gagagcgtgc gcgctcgggc gaacatcgtg
     1441 atctctggag gggccgcgtc ggggaagacg acgatgctcg gcgtcctgtc gtcgttcatc
     1501 ccgccggggg agcggatcat cacgatcgag gacgccgccg agcttcgtct cgcgcagccg
     1561 cacgtcgtgc ggctcgaggc gcggcccgcg aacgtggagg ggaagggcgc cgtcacggtc
     1621 cgggacctcg tccggaacgc gctccggatg cgtccggacc ggctcgtggt cggagaggtc
     1681 cgcggcggcg aggcactcga catgctgcag gccatgaaca ccggacacga gggatcgatg
     1741 tcgaccgcgc acgcgaactc gccgcacgag ctcatgtcgc gcctcgagac gatggctctg
     1801 atgtccgacg tcgacctgcc ggcgctccac gtgcgcgagc agatcggttc gacgatcgac
     1861 ctcgtggtcc acctggcgcg gtttcccgac gggcgcagga tcgtgtctcg gctcgcgcgc
     1921 gtctgcggct accgcgatgg ccgcgtcgtc ctcgaggacg cgctgccgga ggaccggtgg
     1981 gaggtcgcgt gagtccggtg ctcgtcggga tggccgtcgc gctcgcggcg ctgctcgccg
     2041 tcgcagctcg cggcgccagc gcgcgcgagc gtatccgcgc ggcgctcggc gaaccgcatc
     2101 gcggtgacgc c
//
LOCUS       DASZOB010000120         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_103617, whole genome shotgun sequence.
ACCESSION   DASZOB010000120 DASZOB010000000
VERSION     DASZOB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103617"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..769
                     /locus_tag="VG709_01905"
                     /pseudo
     CDS             212..769
                     /locus_tag="VG709_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            821..1408
                     /locus_tag="VG709_01910"
     CDS             821..1408
                     /locus_tag="VG709_01910"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC31865.1"
                     /translation="MRADLGIDGQPLVVFAGRLEYEKGVQTLIDAVPIVDRAAPGVRV
                     VVAGAGSYRAELERRVRALRLGRRVRFEGFVDERRLRRLYQAADAVVVPSLYEPFGLV
                     AVEAMACRTPVVASDTGGLREVVDHGVTGLRFARGNGRSLAAALRRVLTDPELASRLT
                     DEAAAVLAARPSWDEVADRTARAYGRAVERGPVRA"
     gene            1772..2269
                     /locus_tag="VG709_01915"
     CDS             1772..2269
                     /locus_tag="VG709_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31866.1"
                     /translation="MAAIAFGAPVVAVDTNVRRVVGRACLGADDGDVSADDVGRAAAG
                     WLGSSDPAAWNQAVMDLGREVCRPAPRCNECPIADACTYRGTARHRSTRERRVERFEG
                     STRQLRGRIVAELRRSASLTLDELAASADVGRADVAAAVASLAADGLVAEAPGRRGGG
                     IRLAP"
     gene            2300..>4953
                     /locus_tag="VG709_01920"
     CDS             2300..>4953
                     /locus_tag="VG709_01920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC31867.1"
                     /translation="MEALSLLGPVARTWFEEAFGEPTPAQEVAWPALAAGESALVVAP
                     TGSGKTLAAFLVFLDRLAKEPARDRQGVRVLYVTPLRALGNDIYRNLEVPLAGMRDVA
                     ARLGGPEPAITTGIRTGDTPQRERERLVRTPPDVLITTPESLYLMLTSERASKTLADV
                     EAVIVDEVHALAPNKRGTHLALSLERLAWLVGRPFQRVGLSATVRPAERVAAWLGGRE
                     RGRPRDIRVLDAGGRKAIDLLVETSVDDLRELPGESVWPSVFDELAELIRKHRSTLVF
                     VNNRALAERVAAQVNQRAGDDICRVHHGSVSRERRFEAEELLKAGKLPAMATTSSLEL
                     GIDVGAVDLVVQIESPKTVTAGLQRVGRAGHLVGEVAKGRIIPKFRPDLLDAAAVARG
                     MLRREVEETEIPEACLDVLAQQIAAAAAVATWKADALFDLVRGAYPYRDLDRARFEKV
                     VGMLAGRYPAARFGELRPRVVWNREAGEVSARPGTRLLAVNNAGSIPDRGYYAVIHSG
                     SGAKLGELDEEFVFEARPGDTFVLGSAIWRIDGIDDDRVRVTESPGSLPSIPFWHGEA
                     PGRPYELGVRVGKFLHEAGDRLDEPDLVEWLREECALGERAALNLRTYLLEQRDAVGE
                     LATDRTIVVEEFEDELGDRRVTIHSPFGQRVHHSWLLVARTRVRDAENLDIEGVAVDD
                     GLMLRFPGRDGPVPLDLVTALPTGEELEELLLREVQFSPMFGTLFREAAARALLLPRR
                     GPGRRTPLWLQRVRSADLLQITKRLGDFPVLLEAYREAWDDVLRVRDFRRVAEGIASG
                     DIAIHRVERDMPSPFAMHFLFDLNFALQYGGDQPRAEWRSQLMAVDRDLLSRVVRADA
                     LRELLDERALEGVEGLLQRLDERSRPRGR"
BASE COUNT          608 a         1769 c         1873 g          703 t
ORIGIN      
        1 gttcgggcga cgcggcgacg agggcggcga gaccgcgtcg aaggtctggc tcgcggcgct
       61 cacggtcgcg gtcgtcggga tgggcgtggt gcagatccgg cagctccagc gcctgaaccg
      121 cggcgccgtc tagcgacccg cgcggaaggc gtccgcaggc gtccgtatag gatgcgcgca
      181 cggtcgcgca cggcgcgcgg ccggatgcag cgtgcgttcg ctctcggttg ccctgctctc
      241 ctgggagtac ccgcccgcgg tggtcggcgg gctgtcgcgc cacgtctacg agctcgcggg
      301 cgcgctcggg tcccaagggc atcgcgtcac cgtctacacg gggccgagcc cgcgccggcg
      361 tgaggtgccg cgggacggcg tccgcgtcgt ccgcgccagg ccgccctcgc cggagatccc
      421 gttcgaggat ctcgtgccgt gggtcctgtc gttcaacctc gcgttgctcg agcgcgcagc
      481 cgccgacgtc gtcgcacagc caccggacgt gatccacgct cacgactggc gcgtcgctca
      541 caccgccagc gccctccgct agctcaccgg cgcaccgatc gtcgcgacga tccacgcgac
      601 cgagcacggg cgccacgcgg gacggctctc gggcccggtg cagcgcttcg tccacggcgt
      661 cgaggggtgg ctcacctcgg aggccgcgcg ggtcatcacc tgctcggagt tcatgcggga
      721 ggaggtgatc gggctcttcg gcctgacgcc ggagaaggtc gacacgatcc cgaacgaggt
      781 cagccgcgag gcgttcggcg gacggcgccg cggccgctcg atccgtgcgg acctcgggat
      841 cgacgggcag ccgctcgtcg tcttcgcggg gcgtctcgag tacgagaagg gggtccagac
      901 cctgatcgac gccgtcccga tcgtcgaccg cgccgcgccc ggcgtgcggg tcgtggtggc
      961 gggggcgggg agctaccgcg cggagctgga gcgccgtgtc cgcgcgctgc ggctcggtcg
     1021 gcgggtacgg ttcgaggggt tcgtagacga gcggcggctg cgtcgcctgt accaggccgc
     1081 ggacgccgtc gtcgtcccgt cgctgtacga gccgttcgga ctcgtcgcgg tagaggcgat
     1141 ggcctgccgc acgccggtcg tcgcctcgga caccggaggc ctccgcgagg tggtcgatca
     1201 cggcgtcact ggtctgcggt tcgcacgggg gaacggccgg tccctcgccg ccgcgctccg
     1261 tcgcgtgctg accgacccgg agctcgcgtc acggctcacc gacgaggcgg ccgcggtcct
     1321 cgcggcgcgg ccatcctggg acgaggtcgc ggatcgaacc gcgcgcgcgt acgggcgagc
     1381 cgtcgagcgt ggtccggtcc gcgcgtgagc cgcccgagcg ctcgcgccct cctgcgctgg
     1441 tacgcgccgc gagcggcgct ctatccatgg cggcgtcgcc ccacgccgta ccgggtgctc
     1501 gtgagcgagg tgatgctcca gcagacgcag gccgctcgtg tcgtgccggc gttccggcgg
     1561 tgcgtccgtc gtttcccggc gggcgaggcg ctcgcggccg cgccgcgatc ggaggtcgtg
     1621 ggacagtggt ccggcctcgg ctacaaccgt cgcgcggtcg cgctgtcaga ggcggcgcgc
     1681 gcggtcacgc gcgagcacgg aggccgcgtc ccaagcgacc tcgaggcgct gcgccggctt
     1741 ccggtgcgtc ggtccgtaca ccgcctcggc ggtggccgcg atcgcgttcg gggcgccggt
     1801 cgtcgcggtc gacacgaacg tgcggcgcgt ggtggggcgc gcctgcctcg gcgccgatga
     1861 cggcgacgtc agcgccgacg acgtcggccg tgccgccgcc gggtggctcg ggagcagcga
     1921 ccccgcagcg tggaaccagg ccgtcatgga cctcggtcgc gaggtgtgcc ggccggcccc
     1981 gcgctgcaac gagtgcccga tcgcggacgc gtgcacgtac cgcgggacgg cgcgccaccg
     2041 ctcgacgcgt gaacggaggg tcgagcggtt cgagggatcg acgagacagc tccgcggccg
     2101 catcgtcgcc gagctgcgcc gttcggcgtc cctcaccctc gacgagctcg cggcttccgc
     2161 cgacgtgggc cgtgccgacg tggccgcagc ggtcgcgtcg ctcgcggccg acggcctcgt
     2221 cgccgaggcg ccgggccgaa ggggcggcgg catccggctc gccccgtagc gcgtcgcgtc
     2281 gagccgtacg atggctcaga tggaagcgct ctccctgctc gggcccgtgg cgaggacgtg
     2341 gttcgaggag gcgttcggag agccgactcc ggcccaggag gtcgcgtggc ccgcgctcgc
     2401 cgccggcgag tcggcgctcg tcgtcgcgcc gacgggttct ggaaagacgc tcgcggcgtt
     2461 cctcgtgttc ctcgaccgcc tcgcgaagga gcccgcgcgt gaccgtcaag gcgtccgcgt
     2521 cctctacgtg acgccgctcc gcgcgctcgg caacgacatc taccggaacc tggaggtccc
     2581 gctcgccggg atgcgcgacg tcgccgcgcg tctcggcggg cccgagccgg cgatcacgac
     2641 cggcatccgg accggcgaca cgccgcagcg cgagcgggag cggctcgtgc gaactccgcc
     2701 cgacgtgctg atcacgacac cggagtcgtt gtacctcatg ctcacgtccg aacgcgcgtc
     2761 gaagacgctc gccgacgtgg aggccgtgat cgtcgacgag gtccatgccc tcgcgccgaa
     2821 caagcgcggg acgcacctgg cgctctcgct cgagcggctc gcgtggctcg tcgggcggcc
     2881 gttccagcgc gtcggtctct ccgccaccgt ccgtcccgcc gagcgcgtcg cggcgtggct
     2941 cggagggcgg gagcgcggtc gcccgcgcga catccgcgtc ctggatgccg gcggccgtaa
     3001 ggcgatcgat ctcctcgtcg agacctccgt cgacgacctc cgggagctcc cgggggagtc
     3061 cgtgtggccg agcgtgttcg acgagctcgc cgagctgatc cggaaacacc ggtcgacgct
     3121 cgtcttcgtc aacaaccggg cgctcgcgga gcgggtcgcg gcgcaggtca accagcgcgc
     3181 cggcgacgac atctgtcgcg tccatcacgg gagcgtgtcg agggagcgcc gcttcgaggc
     3241 ggaggagctc ctcaaggccg ggaagttgcc ggcgatggcg acgacgtcgt cgctcgagct
     3301 cggtatcgac gtcggcgccg tagacctcgt cgtgcagatc gagagcccca agaccgtgac
     3361 ggcagggtta cagcgcgtcg ggcgcgccgg gcacctcgtg ggcgaggtgg cgaagggcag
     3421 gatcatcccg aagttccgcc cggacctcct ggacgcggcc gccgtcgcgc gcggcatgct
     3481 tcgccgcgag gtcgaggaga cggagatccc ggaagcgtgc ctcgacgtgc tcgcccagca
     3541 gatcgcggcg gccgcggccg tcgcgacgtg gaaggccgac gcgctattcg acttggttcg
     3601 cggcgcgtac ccgtaccgcg atctcgaccg cgcgcggttc gagaaggtcg tggggatgct
     3661 ggccggccgc taccccgcgg ctcggttcgg cgagctccgc ccgcgcgtcg tatggaaccg
     3721 cgaggcgggc gaggtgagcg cacgcccggg gacgcggctg ctcgcggtca acaacgccgg
     3781 ctcgatcccg gaccgcgggt actacgcggt gatccactcg gggagcggcg cgaagctcgg
     3841 cgagctcgac gaggagttcg tgttcgaggc gaggcccggc gacacgttcg tcctcggctc
     3901 ggcgatctgg cgcatcgacg ggatcgacga cgaccgcgtc cgcgtcaccg agtcgccggg
     3961 atcgcttccg tcgatcccgt tctggcacgg cgaggcgccg gggcgtccgt acgagctcgg
     4021 cgtgcgcgtc gggaagttcc tccacgaggc cggagaccgg ctcgacgagc ccgacctcgt
     4081 cgagtggctg cgcgaggagt gcgcgctggg cgagcgagcg gcgctgaacc tccggacgta
     4141 cctcctcgag cagcgtgacg ccgtcggaga gctcgccacc gaccggacga tcgtcgtcga
     4201 ggagttcgag gacgagctcg gcgaccgtcg cgtgacgatc cactctccgt tcggccagcg
     4261 cgtccatcac tcgtggctcc tcgtcgcgcg gacgcgtgtc cgcgacgcgg agaacctcga
     4321 catcgagggc gtcgcggtgg acgacggcct catgctgcgg ttcccaggcc gcgacggacc
     4381 ggtgccgctc gacctcgtca cggcgctgcc gaccggcgag gagctcgagg agctgctgct
     4441 gcgtgaggtt cagttctcgc cgatgttcgg gacgctgttc cgcgaggcgg ccgcgcgagc
     4501 gctgctcctg ccccgacgag gtcctgggag acgcactccg ctctggctcc agcgcgtgcg
     4561 ctccgccgac ctgctgcaga tcacgaagcg cctcggcgac ttcccggtcc tgctcgaggc
     4621 gtaccgcgag gcgtgggacg acgtgctccg cgtgcgcgac ttccgccgcg tcgccgaagg
     4681 gatcgcgtcg ggggacatcg cgatccaccg cgtcgagcgc gacatgccgt cgccgtttgc
     4741 gatgcacttc ctgttcgacc tgaacttcgc gctgcagtac gggggcgacc agccgcgtgc
     4801 cgagtggcgc agccagctga tggccgtcga ccgcgacctg ctctcgcgag tcgtccgcgc
     4861 cgacgcactg cgcgagctcc ttgacgagcg tgcgctcgag ggcgtcgagg ggctgctgca
     4921 gcggctcgac gagcgatcgc ggccgcgcgg ccg
//
LOCUS       DASZOB010000121         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_164017, whole genome shotgun sequence.
ACCESSION   DASZOB010000121 DASZOB010000000
VERSION     DASZOB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164017"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VG709_01925"
     CDS             complement(<1..225)
                     /locus_tag="VG709_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31868.1"
                     /translation="MSATATDAPATQRTRALFRIPDVRLRSAWHLWQRNAAIYRRTYK
                     LNLLPNFFEPVFFLLAMGFGLGAAVGRVAGF"
     gene            complement(222..1139)
                     /locus_tag="VG709_01930"
     CDS             complement(222..1139)
                     /locus_tag="VG709_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC31869.1"
                     /translation="MSAVVAKGLVKRFGDVEAVRGIDFEVADQECFGFLGPNGAGKTT
                     TMRMISCTSPPTDGDLTVLGKTVERGRDIKRRMGVVPQENNLDEEVTVVENLLIYARY
                     FDLHPRIARPTADELLHFVALQDKRDWRIERLSGGMKRRLLIARALMNEPDILLLDEP
                     TTGLDPQARHLVWEKLRSLKRQGVTLLLTTHYMEEAAQLCDRLVIMHEGRILIEGSPR
                     ELVARETSPQVIEVLDPSPEVARGLEKLDGVVDRVERLADRWLFYTKDGDAVLGEIRG
                     VVKEPAAVWLRGSTLEDVFLQLTGRGLLE"
     gene            complement(1219..2265)
                     /gene="menC"
                     /locus_tag="VG709_01935"
     CDS             complement(1219..2265)
                     /gene="menC"
                     /locus_tag="VG709_01935"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWC31870.1"
                     /translation="MPLIRAFRTSFGTTTTKEVILVRAIGTDGVVGWGECVAAEAPRY
                     SGEWNAGAWIVLRDFLVPAALEGHDAGIRGHPMAYAALEAAFADLDLRRKGVSLSEHL
                     GGASDRVPCGVSVGIENDLDALVDVVASFVDLGYRRVKLKIEPGRDVDVVSAVRDAFP
                     DVPLSVDANAAYRRDTWDALLALDDLELEYLEQPLPEDDLLGHIELQRRLATPICLDD
                     TITSARAARAAIELRACGVINVKVGRVGGLSESLAIHEVATAAGVPLWCGGMLETGIG
                     RAVNVAVASLPGFTLPGDTSGSDRYFERDVTEPFVVEADGTMAVPDGPEIGVEPVPEI
                     LEEIEVERTIVRHE"
BASE COUNT          356 a          835 c          814 g          337 t
ORIGIN      
        1 gaatcccgcg acgcgcccga cggcggcgcc gaggccgaac cccatcgcga gcaggaagaa
       61 gaccggctcg aagaagttcg gcagaaggtt gagcttgtac gtgcgacggt agatcgcggc
      121 gttccgctgc cacaggtgcc acgccgaccg gagccggacg tccgggatac ggaacagcgc
      181 tcgcgtcctc tgcgtcgccg gcgcgtccgt cgccgtggcg ctcactcgag cagccccctg
      241 ccggtgagct ggaggaacac gtcttcgagc gtggagccgc ggagccagac cgccgcgggc
      301 tccttcacga cgccccggat ctcccccagc acggcgtcgc cgtccttcgt gtagaagagc
      361 caccggtcgg cgagccgctc gacgcggtcg acgacgccgt cgagcttctc gaggccgcgc
      421 gcaacctcgg gtgaggggtc gaggacctcg atcacctgcg gcgaggtctc gcgcgcgacg
      481 agctcgcgcg gcgacccctc gatcaggatc cgtccctcgt gcatgatcac gagccggtcg
      541 cagagctgcg cggcctcctc catgtagtgc gtcgtgagca gcagcgtgac gccttggcgc
      601 ttcagcgacc gcagcttctc ccagacgagg tggcgtgcct gcgggtccag accggtcgtc
      661 ggctcgtcga ggagcaggat gtcgggctcg ttcatgagcg cacgggcgat cagcaggcgg
      721 cgcttcatcc ccccggagag ccgctcgatc cgccagtcgc gcttgtcctg gagcgcgacg
      781 aagtgcagca gctcgtccgc ggtcggtcgc gcgatccgcg ggtgcaggtc gaagtagcgc
      841 gcgtagatca ggaggttctc gacgaccgtg acctcctcgt cgaggttgtt ctcctgcggc
      901 acgacgccca tccgtcgctt gatgtcgcga ccccgctcga ccgtcttccc gagcacggtg
      961 aggtcgccgt cggtcggggg agaggtgcac gagatcatgc gcatcgtcgt ggtcttgccg
     1021 gcgccgttcg ggccgaggaa gccgaagcac tcctggtccg cgacctcgaa gtcgatgccg
     1081 cgcacggcct cgacgtcgcc gaagcgcttg acgagcccct tcgcgacgac tgcgctcatg
     1141 ggagtgtggg atcggaaaca ggagggaagg agcggaccac cgaaccgagt ctagcagcgg
     1201 cccctttcct gaccggcctc actcgtgccg gacgatcgtg cgctcgactt cgatctcctc
     1261 gaggatctcg gggaccggct cgacgccgat ctcgggcccg tccggaaccg ccatcgtgcc
     1321 gtccgcctcg acgacgaacg gctcggtgac gtcgcgctcg aagtaccggt cggagccgct
     1381 cgtgtctccc ggaagcgtga accccgggag ggaggcgacc gcgacgttga ccgcgcgacc
     1441 gatcccagtc tcgagcatcc cgccgcacca cagcgggacc ccggcggccg tcgcgacctc
     1501 gtgtatcgcg agcgactcgg agagcccgcc gacgcgtccg accttcacgt tgataacccc
     1561 gcacgcccgc agctcgatcg cggcgcgcgc ggcgcgggcg ctcgtgatcg tgtcgtcgag
     1621 gcagatcggc gtggcgaggc gtcgctggag ctcgatgtgg ccgagcagat cgtcctcggg
     1681 aagcggctgc tcgaggtact ccagctcgag atcgtcgagc gcgaggagcg cgtcccacgt
     1741 gtcgcgccgg taggccgcgt tcgcgtcgac cgacagcggc acgtcgggga aggcgtcgcg
     1801 cacggcgctc acgacgtcga cgtcgcgccc cggctcgatc ttgagcttca cacgccggta
     1861 gccgaggtcg acgaacgacg cgacgacgtc gacgagcgcg tcgagatcgt tctcgatccc
     1921 gacgctcacg ccgcacggga cgcggtcgct cgcgccgccg aggtgctccg acagcgagac
     1981 gcccttgcgc cggaggtcaa gatcggcgaa cgcagcctcc agcgccgcgt atgccatggg
     2041 gtgcccacgg atgccggcgt cgtgcccctc gagggccgcc ggcaccagga agtcgcggag
     2101 gacgatccac gcgccggcgt tccactcccc tgagtaccgc ggcgcctcgg cggcgacgca
     2161 ctcgccccac ccgacgacgc cgtcggtccc gatcgcgcgg acgaggatca cctccttcgt
     2221 cgtcgtggtt ccgaacgacg tgcggaaagc ccggatgagc ggcatccgaa cgaggacgag
     2281 ctcgacggcc gcgagccgct cagtccgcac ggcgctcacg acgtcgacgt cgcgccccgg
     2341 ct
//
LOCUS       DASZOB010000122         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_103695, whole genome shotgun sequence.
ACCESSION   DASZOB010000122 DASZOB010000000
VERSION     DASZOB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103695"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VG709_01940"
     CDS             complement(<1..1589)
                     /locus_tag="VG709_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWC31871.1"
                     /translation="MLWRYRLWPLAAAFLVTLSGTAHAQARSSAALAPPVDAVIARYF
                     DDPASPYGPGHRGIDYAVAAGTRVRAAGDGVVSWAGSVAGNLSVTIDHGSGLETTYSM
                     LASLEVTRGEAVDEGRFLGTAGRDHDGASAGLHFGVKLHDDYVDPLDHLGPVGLGDAI
                     HLAPLVEETAPEVPHVARLAFEGAGAHVRPCRDPGSPGRTAPPPNDNVAVAVAGLSSN
                     TIGKTNAAIYEEHNGPAGLGYPAKRIYRFSYRGVEGPRLHEPYEASDTWTDIDKSGRR
                     LAALLRAIHERHPRAEVDLFAHSQGGLVARAYLERLAGAYDPRLPRVEHLVTYGTPHR
                     GTPLAGVAGELDEHTLTGRFVVDRLSEWAREDGPALDPRAPSVSQMEPGSDFLAELAR
                     EDVVFGTRVLSLAMPHDIVVPAESALYPGKEARVLSPRGVNGHEAVVVADEARALAYS
                     FLRDAPVSCRGAWDEWGRVVGRGIGFVHGLMDDAYRHLETFGVTKAATLVGRAAKAAW
                     SGVKWAGRGLKRVGSDAVRAGG"
BASE COUNT          285 a          651 c          617 g          249 t
ORIGIN      
        1 ccccccgcgc gcacggcatc ggagccgact cgctttaagc cccggcccgc ccacttcacg
       61 ccgctccacg ccgccttcgc cgcacggccg acgagcgtcg ccgccttcgt gacgccgaac
      121 gtctcgaggt gccggtaggc atcgtccatg aggccgtgga cgaaaccgat cccgcggccg
      181 accacgcggc cccactcgtc ccaggcgccc cggcacgaga ccggcgcgtc gcgcaagaac
      241 gagtacgcga gggcgcgtgc ctcgtcggcc acgacgaccg cctcgtgccc gttgacaccg
      301 cggggcgaca ggacgcgcgc ttctttaccc gggtagagcg ccgattcggc agggacgacg
      361 atgtcgtgcg gcatcgcgag cgacagcaca cgcgtgccga agaccacgtc ctcgcgcgca
      421 agctccgcca ggaaatccga acccggttcc atctgcgaga cggagggggc gcgcggatcg
      481 agagccggcc catcctcgcg cgcccactct gagagacgat cgaccacgaa tcgcccggtg
      541 agcgtgtgct catcgagctc gccggcgacg cctgcgagtg gtgtccccct atgaggcgtc
      601 ccataggtga cgaggtgctc gacgcgcggc aggcgcggat cgtatgcgcc ggccagacgc
      661 tcgaggtaag cccgcgcaac gaggccgccc tgcgaatggg cgaagagatc gacctccgct
      721 cggggatggc gctcgtggat agcgcgcagc aacgccgcga ggcggcgccc gctcttgtct
      781 atgtcggtcc aggtatcgct cgcctcatag ggctcgtgca gtcggggacc ttctacccct
      841 cgatacgaga agcggtagat cctcttcgcc gggtacccga gacccgcggg cccgttgtgc
      901 tcttcgtaga tcgctgcgtt cgtcttgccg atggtgttgc tcgacagacc ggcaaccgcg
      961 accgcgacgt tgtcgttggg aggcggggcc gtacgccccg gactcccggg atcccgacac
     1021 ggacgcacgt gcgcgccggc gccctcgaac gcgaggcgcg ccacgtgggg aacctcggga
     1081 gcggtctcct cgacgagcgg cgcgaggtgg atcgcgtcgc cgagcccaac ggggccgaga
     1141 tggtcgagcg ggtcgacgta gtcgtcgtgc agcttcacgc cgaagtgcag cccggccgag
     1201 gccccgtcgt gatcccgccc ggcggttccc agaaagcgtc cttcgtccac ggcctcgccg
     1261 cgcgtgacct cgagtgaggc gagcatcgag taggtcgtct ccagaccgga tccgtggtcg
     1321 atcgtgaccg agaggttgcc ggcgacgctg cccgcccagg agacgacgcc gtcgccggcg
     1381 gcgcgcacgc gcgtccccgc cgcaaccgcg taatcgatcc cgcgatgtcc gggaccgtaa
     1441 ggagatgctg ggtcgtcgaa gtaacgcgcg atgacggcgt ctacgggagg cgccagcgct
     1501 gccgacgaac gcgcctgcgc gtgtgccgtc ccggacaacg tgacgaggaa tgcggccgca
     1561 aggggccaca accggtagcg ccagagcatc tcgtcacgct gaagcgaaaa gtcgtcgcct
     1621 gctgtgatcg ggctcaccga cgcgagcccc gggcccggat caggggacga tgcgcgtccc
     1681 gctctcgcca cgcagccggt cctcgcagcg ctcgatcgac gtgatgatgc ccggccgccc
     1741 cgttgcctcg acgaagcggc acaccgcctc gaccttgggc tccatcgacc ccgccgggaa
     1801 ct
//
LOCUS       DASZOB010000123         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_172936, whole genome shotgun sequence.
ACCESSION   DASZOB010000123 DASZOB010000000
VERSION     DASZOB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172936"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VG709_01945"
     CDS             complement(<1..1296)
                     /locus_tag="VG709_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWC31872.1"
                     /translation="MAVRAEPATVPSWSEGPVLSWLTTVDHKRIGIMYIVTAFVFFLA
                     GGVMALLMRLQLMQADLDIVTKDAYNQLFTIHGTAMIFLFVVPVLAGVANYLVPLMIG
                     ARDMAFPRLNAFSYWLFLAGGLVLIASFFADRGAAQGGWYSYPPFTSDELFGSPFVGQ
                     DLWILAIHLTTIASIAGAINFVVTIHNMRAAGMTWMRMPLFVWTIEAYAVLLVLALPT
                     LTAAVTLLLLDRQAGTAFFMPDAGGAQVLYQHMFWFFGHPEVYIMILPAFGMVSEIVP
                     VFSRKPIFGYKAIAFATIGIAFYGMLVWAHHMFTVGLPTWLQGTFMITSFAVAVPTGI
                     KIFNWLATLWRGQLLFRAPLLFCLGLIGLFTIGGLSGIFLAAFPYDYQAHDSYFVVAH
                     MHYVLCGGAVFALFAALYYWWPKIFGRMLHRGLGKLQFWV"
     gene            complement(1286..>2285)
                     /gene="coxB"
                     /locus_tag="VG709_01950"
     CDS             complement(1286..>2285)
                     /gene="coxB"
                     /locus_tag="VG709_01950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWC31873.1"
                     /translation="VGLLAAVLIVLPLTFGMPPGWGLPEPVTPSGRAIDDIYWVVFGL
                     AAFVFLLVEGALLLFIFRFRRRPDAREDVEGPQIHGNTRVEIVWTLVPVLLLVAIAAF
                     VLAQLPTVQAAPADGQGERIRVTAHQFYWQYEYPNGALSFDALYLPVDRNVSLALESA
                     DVVHSWWVPKLTGKRDAIPGRTNVLSFRPTRTGALENGKCGEFCGIQHARMLTRVEVL
                     PQEEYDRWLDENAPDGGDDLAALGEAEWAAACAKCHGPEGEGDIGPPIAGNGALREAD
                     SLRRLLYEGQDLDSNPGYMPPVGRGWSDRQIEALLAYVEATPALAEPPQGGETDGG"
BASE COUNT          417 a          736 c          828 g          304 t
ORIGIN      
        1 cacccagaac tgcagcttgc cgaggccgcg gtgcaacatg cggccgaaga tcttaggcca
       61 ccagtagtac agcgcggcga acagcgcgaa cacggcgccg ccgcagagga cgtagtgcat
      121 gtgcgcgacg acgaagtacg agtcgtgcgc ctggtagtcg taggggaacg ccgccaggaa
      181 gatccccgag agcccgccga tcgtgaagag gccgatgagg ccgaggcaga agaggagcgg
      241 cgcgcggaag agaagctggc cacgccagag cgtcgcgagc cagttgaaga tcttgatccc
      301 ggtcgggacg gcgacggcga acgacgtgat catgaacgtc ccctgcagcc acgtcggaag
      361 ccccacagtg aacatgtggt gcgcccacac gagcatcccg tagaaggcga tgccgatcgt
      421 cgcgaacgcg atcgccttgt aaccgaagat cggcttgcgc gagaagaccg ggacgatctc
      481 ggacaccatc ccgaaggcgg gcaggatcat gatgtagacc tcggggtgcc cgaagaacca
      541 gaacatgtgc tggtagagca cctgggcgcc gccggcgtcc ggcatgaaga acgccgtccc
      601 ggcctggcgg tcgaggagga ggagcgtgac ggccgccgtc agcgtcggga gcgcgaggac
      661 gagcaggacg gcgtacgcct cgatcgtcca gacgaagagc ggcatccgca tccacgtcat
      721 cccggctgcg cgcatgttgt ggatcgtcac gacgaagttg atcgcgcccg cgatcgaggc
      781 gatcgtcgtc aggtggatcg cgaggatcca gaggtcctga ccgacgaagg ggctcccgaa
      841 gagctcgtcg ctcgtgaacg gcgggtacga gtaccagccc ccctgcgcgg cgccgcggtc
      901 ggcgaagaag ctcgcgatca ggacgagccc gccggcgagg aagagccagt acgagaacgc
      961 gttcaggcgc gggaacgcca tgtcgcgcgc gccgatcatg agcgggacga ggtagttcgc
     1021 gacgccggcg agcaccggaa cgacgaaaag gaagatcatc gccgtcccgt ggatcgtgaa
     1081 gagctggttg taggcgtcct tcgtgacgat gtcgaggtcc gcctgcatga gctggaggcg
     1141 catgaggagc gccatcacgc cgcccgcgag gaagaagacg aacgccgtga cgatgtacat
     1201 gatcccgatc cgcttgtggt ccacggtcgt gagccagctc aggaccgggc cttcgctcca
     1261 gctcgggacg gttgcgggct ccgccctaac cgccatcggt ctcgcctcct tgcggcggct
     1321 cggcgagcgc cggcgtcgcc tcgacgtagg ccagcagcgc ttcgatctgg cggtcgctcc
     1381 atccccgccc gacgggaggc atgtagccgg ggttcgaatc gaggtcctgg ccctcgtaca
     1441 ggagacgccg gaggctgtcg gcttcgcgga gcgccccgtt gccggcgatc ggcggcccga
     1501 tgtcgccctc gccctccggc ccatggcact tggcgcaggc ggccgcccac tccgcttcgc
     1561 cgagcgcggc gagatcgtcg ccgccgtccg gcgcgttctc gtcgagccac cggtcgtact
     1621 cctcctgcgg aagcacctcg acgcgcgtca gcatgcgcgc gtgctggatc ccgcagaact
     1681 cgccgcactt gccgttctcg agcgcgcccg tccgcgtggg ccggaacgag aggacgttcg
     1741 tgcggccggg aatcgcatcc cgcttcccgg tcagcttggg gacccaccag ctgtgcacga
     1801 cgtcggccga ctcgagcgcg agcgagacgt tgcggtcgac cgggaggtag agcgcgtcga
     1861 agctgagggc gccattcggg tactcgtact gccagtagaa ctggtgcgcg gtgacgcgga
     1921 tccgctcgcc ctggccgtcc gcaggggcgg cctggacggt cgggagctgc gcgagcacga
     1981 acgccgcgat cgcgacgagg aggagcacgg gcacgagcgt ccacacgatc tcgacgcgcg
     2041 tgttgccgtg gatctgcggg ccctcgacgt cttcccgggc gtcggggcgg cggcggaagc
     2101 ggaagatgaa gaggaggagc gcgccctcga cgagcaggaa gacgaacgcg gcgagtccga
     2161 agacgaccca gtagatgtcg tcgatggccc ggccgctcgg cgtgacgggc tcgggaagcc
     2221 cccagccggg cggcatgccg aaggtaagcg gcaggacgat caagacggcc gcgagcagcc
     2281 cgacg
//
LOCUS       DASZOB010000124         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_143747, whole genome shotgun sequence.
ACCESSION   DASZOB010000124 DASZOB010000000
VERSION     DASZOB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143747"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="truB"
                     /locus_tag="VG709_01955"
     CDS             complement(<1..627)
                     /gene="truB"
                     /locus_tag="VG709_01955"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWC31874.1"
                     /translation="MTVAAEMALAADELAAGGLVLADKERGPSSFAVVRRGRRRFGLK
                     AGHAGTLDPFATGLLVCLLGPATRLARYLVGVDKRYVTTIRLGVRTDTGDDEGTKTEE
                     TALPTREVIEALAGDVDLRIPTASAVKVGGERAYRLRRRGVALEMPTRRSTIHRLDVV
                     RFEPPELELDLHVSSGTYVRSIADALGGHCVALRRLAVGQLRVEDADEE"
     gene            complement(819..>1516)
                     /locus_tag="VG709_01960"
     CDS             complement(819..>1516)
                     /locus_tag="VG709_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HWC31875.1"
                     /translation="GRLLEPARFVVNIDHHHDNTRFGDVNVIRPDASSTGEILRDLLT
                     ELDVALTPGIAEALYTAIVTDTGRFQYSNTTAKALRLAAELVEAGANVHRVFQGVYEN
                     VAFAKLKLLARALDRARVLEGGRVVVSYLERGDFEDAGAEEPFSEGIIDYLRAVEGAQ
                     VAALIREPPTRNGPSRRVSLRTTEETLDVSAIARKSGGGGHKQAAGFPSEASVGEITD
                     FIRREFLAQTGPA"
BASE COUNT          209 a          526 c          569 g          212 t
ORIGIN      
        1 ttcctcgtcg gcgtcctcca cgcgaagctg cccgacggcg aggcgccgga gcgcgacgca
       61 gtggcccccg agcgcgtctg cgatcgagcg cacgtacgtg ccgctcgaga cgtgcaggtc
      121 gagctcgagc tcgggcggct cgaaccggac gacgtccagc cggtggatcg tggagcggcg
      181 ggtcggcatc tcgagggcga ccccgcggcg ccggagccgg taggcgcgct cgccgccgac
      241 cttgaccgcc gacgcggtcg ggatgcgaag gtcgacgtcg cccgcgagcg cctcgatcac
      301 ctcgcgcgtc ggcaaggcag tctcctccgt cttcgttccc tcgtcgtcgc ccgtgtcggt
      361 gcgcacgccg agccgtatgg tcgtgacgta gcgcttgtcg actcccacga ggtagcgcgc
      421 aagccgcgtg gcggggccta ggaggcagac gaggagcccg gtggcgaagg gatcgagcgt
      481 tccggcgtgc cctgccttca gcccgaaccg ccgccgaccg cgacggacga ccgcgaagga
      541 cgagggtccg cgctccttgt cggcgagaac gagcccaccg gcggcgagct cgtcagccgc
      601 gagcgccatc tcggccgcaa cggtcacgaa gtcgtgacga atctggcgtc acacgcgcga
      661 ttctcgccga tacgcgggac cgcgaggcgg gaacacacgg gggtccccgg ggggcacagc
      721 gcgccggtct gggcgcgagc tcgcgcgggc gcgcgtgcga ggtgcggagg gccccgcagg
      781 atcggcaccg ggcgcgcgtt cagcggacgg ccgctgcgtt acgccgggcc ggtctgggcc
      841 aggaactcgc gacggatgaa gtcggtgatc tcccccacgc tcgcctcgga cgggaacccc
      901 gcggcctgct tgtggccgcc gcccccgctc ttgcgcgcga tcgcggagac gtccagggtc
      961 tcctccgtcg tgcggaggct gacgcggcgg ctcggtccgt tgcgcgtcgg aggctcgcgg
     1021 atcagtgccg cgacctgcgc gccctcgacg gcccggaggt aatcgatgat tccctcggag
     1081 aagggctcct ccgcccccgc gtcctcgaag tcgccgcgct cgaggtacga gacgacgacg
     1141 cgcccgccct cgaggacgcg cgcccgatcg agagcgcgcg cgaggagctt gagcttggcg
     1201 aacgcgacgt tctcgtacac gccctggaag acgcggtgga cgttcgcgcc ggcctcgacc
     1261 agctcggcgg cgagccggag cgccttggct gtcgtattcg agtactggaa gcggccggtg
     1321 tcggtcacga tcgccgtgta gagcgcctct gcgatcccgg gcgtcagggc gacatcgagc
     1381 tccgtgagca gatcgcgcag gatctcgccg gtcgacgagg cgtcgggacg gatgacgttc
     1441 acgtcgccga agcgcgtgtt gtcgtggtga tggtcgatgt tgacgacgaa ccgtgcgggc
     1501 tcgagcagcc ggcctg
//
LOCUS       DASZOB010000125         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_53134, whole genome shotgun sequence.
ACCESSION   DASZOB010000125 DASZOB010000000
VERSION     DASZOB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53134"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VG709_01965"
     CDS             <1..338
                     /locus_tag="VG709_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31876.1"
                     /translation="LVWTDAPGHGLGGTTPAWVNDLDLRVTAEDVEYLGNWFGEPSSG
                     WSVPGGTADFMNNVENVFLPSARGVVQIDVLAANLAGDGVPGIGDETDQDFALVISNA
                     RLGKAKDKR"
     gene            385..996
                     /locus_tag="VG709_01970"
     CDS             385..996
                     /locus_tag="VG709_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWC31877.1"
                     /translation="MATAHVLERRDGIVRGAAQLFSARGYHATSMRDLAEHIGIRAPS
                     LYAHVESKEDLLYEIVAGAAEQFIGGLGRVLESSATAEEKLRAAMRSHVRVLADNLEG
                     ARVFHHEWRALRSPRREEIEVLRDRYEEMWDAVLRELRPVGSRFARLLALSAANWTYV
                     WYDPAGAMSPEEVADRFTDLLLDGLRTTATGKDRKRADRGATR"
     gene            993..1907
                     /gene="paaA"
                     /locus_tag="VG709_01975"
     CDS             993..1907
                     /gene="paaA"
                     /locus_tag="VG709_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWC31878.1"
                     /translation="MTYEERRAEFKARIDAGEKIEAGDWMPDEYRQAALKFIEMHANS
                     EIMGCLPEREWIPRAPNLRRKLSLTAKVQDEAGHAQILYRVAEDLGKSREQMFDDLVN
                     GRTKFHNVFHYPTESWGDVAVIGWLIDGAALVTQRALLDCSYSSYVRAMRRICAEESV
                     HLRHGEDLTLELVSGTEEQREMFQDAVDRWWRPIMHFFGPPSKPDKDVLLYWGIKTKS
                     NEELRQEFFTTYVPKLWDLGISVPDPELRFDEERGEWSWSDPGWDDFWQVVRGDGPMT
                     KTRLLYRKAMWDHHSWVREVFAGIPHAA"
     gene            1912..2271
                     /locus_tag="VG709_01980"
     CDS             1912..2271
                     /locus_tag="VG709_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31879.1"
                     /translation="MHIWDVLSRRRDDEPWEAVGSVNAPDLDLAMMLARETHFRHGEG
                     TTYALTQRGDDKIHPCPDPTGIGGVIDRAYRRSEGYAGVGAKLRRVHEAMRERGLVID
                     RPRPPRHRARAEISHGS"
     gene            2261..3133
                     /gene="paaC"
                     /locus_tag="VG709_01985"
     CDS             2261..3133
                     /gene="paaC"
                     /locus_tag="VG709_01985"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWC31880.1"
                     /translation="MAPDPRLELLLALADDELVLGHRHAEWTGWAPYVEEDLAFSSIA
                     QDEIAHARLLYEIAEPLARRDPDALALGREPNEYRNAILCERPNRDWGYTVARQYLYD
                     TADDVRLRALEGSSLAELAHAVRTIRLEERYHLEHAHQWFRRIAHGPSLEARERFAAG
                     LEAAIAEAVALFEALPSEEELVADGVLPRSNEDLLGEWLSALGEALEEASLDWVLTEH
                     ARQAGEMVPTSSGEIEAGPTLAVPGVERRDGRWVHVGGFAGAGGRRGRRSEEFQALWE
                     EMTELYRALPGARW"
     gene            3127..3636
                     /gene="paaD"
                     /locus_tag="VG709_01990"
     CDS             3127..3636
                     /gene="paaD"
                     /locus_tag="VG709_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWC31881.1"
                     /translation="MVTETTSVLDDVRAAVARVRDPEIPAGTIADLGMIHDVRVAGDA
                     VEIELLPTFVGCPAKDVIGEDVRRAAEPVADRPVGVRFVYDPPWTTDRITEQGRARLR
                     EFGIAPHWERGPEPVPIPLLSRAGVQCPFCGSEETVMDSAWGVTPCRSTHYCRSCRNP
                     FEGFKTKSL"
     gene            3707..4219
                     /locus_tag="VG709_01995"
     CDS             3707..4219
                     /locus_tag="VG709_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWC31882.1"
                     /translation="MGRRALLALLVVATLVAGIASLATAGHGGFHATLDGYEEVPAIS
                     TTGEGTFRAEIVSTAKGDVIRYELTYSGLEADATAAHIHFGQPAVAGGVIAWLCGGGG
                     KPACPPRAGTVRGTITPGDIIGPENQGIAPGEFGEAVRAMRAGVTYANVHSSMFPTGE
                     IRGQIGRDFT"
     gene            complement(4293..4628)
                     /locus_tag="VG709_02000"
     CDS             complement(4293..4628)
                     /locus_tag="VG709_02000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC31883.1"
                     /translation="MDARDQREFRAHPSALADVRRWVRSLASGRDLQPSLTEELVIAV
                     NEACTNSVLHSTAHNVEIFWKEDPNLVEVEIRDGGVFRPPAMVGPEEETGRGIPVMLA
                     ASDEFAMKK"
     gene            complement(4690..>4869)
                     /locus_tag="VG709_02005"
     CDS             complement(4690..>4869)
                     /locus_tag="VG709_02005"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HWC31884.1"
                     /translation="LPTRALVASRELMLGALTTGLEEALEREGELQAQMAESHDHLEG
                     VMAFAEKREPKFEGR"
BASE COUNT          758 a         1629 c         1774 g          708 t
ORIGIN      
        1 cgctggtgtg gacggacgcg cccggacacg gtctgggagg caccacgccg gcctgggtga
       61 acgacctcga ccttcgggtg accgccgagg acgtcgagta cctcgggaac tggttcgggg
      121 agccgagcag cggctggagc gtgccgggag ggaccgcgga cttcatgaat aacgtggaga
      181 acgtgttcct gccttcggca aggggcgtcg tccagatcga cgtcctcgcg gcgaatctcg
      241 ccggcgatgg ggtgcccggg atcggcgacg agaccgacca ggacttcgcg ctcgtgatct
      301 cgaacgcgcg gctcggcaag gccaaggaca agcggtaggc cggggggcgg tcctaacgaa
      361 cgttcgttag gtataatggg cggcatggct accgcccacg tgctcgagcg gcgagacggg
      421 atcgttcgcg gcgccgcgca gctgttcagc gcgcgtggct accacgcgac gagcatgcgc
      481 gacctggccg agcacatcgg gatccgcgcg ccgagcctct acgcgcacgt cgagagcaag
      541 gaagaccttc tctacgagat cgtcgccggg gctgccgaac agttcatcgg cgggctcggc
      601 cgcgtgctcg agtcgagcgc cacggcagag gagaagctcc gtgccgcgat gcgctcgcac
      661 gtccgcgtgc tggccgacaa cctcgagggg gcgcgcgtct tccaccacga atggcgcgcg
      721 ctgcgctcgc cgcggcgcga ggagatcgag gtgctgcgcg accggtacga ggagatgtgg
      781 gacgccgtgc tgcgggagct acgaccggtc gggtcgcggt tcgcgcggct gctcgcgctc
      841 tcggcggcga actggaccta cgtctggtac gaccccgcgg gcgcgatgtc gccagaggag
      901 gtcgccgacc ggttcaccga ccttctgctc gatggcctgc gcacgaccgc gacggggaag
      961 gataggaagc gagccgaccg aggagcgacg cgatgacgta cgaggagcga cgggccgagt
     1021 tcaaggcgcg gatcgatgcc ggcgagaaga tcgaggccgg cgactggatg ccggacgagt
     1081 atcggcaggc cgcgctgaag ttcatcgaga tgcatgcgaa ctccgagatc atgggatgcc
     1141 tcccggagcg cgagtggatc ccgcgcgccc cgaacctccg ccgcaagctc tcgctgaccg
     1201 cgaaggtcca ggacgaggcc gggcacgccc agatcctcta ccgcgtcgcc gaggacctcg
     1261 ggaagagtcg cgagcagatg ttcgacgacc tggtgaacgg caggacgaag ttccacaacg
     1321 tcttccacta cccgaccgag tcgtggggcg acgtcgcggt gatcggctgg ctgatcgacg
     1381 gcgccgcgct cgtgacgcag cgcgcgctgc tcgactgctc gtactcatcg tacgtgcgcg
     1441 cgatgcggcg gatctgcgcc gaggagtcgg tccacctccg ccacggtgag gacctcacgc
     1501 tcgagctcgt ctcgggcacg gaggagcagc gcgagatgtt ccaggatgcc gtcgaccgct
     1561 ggtggcggcc gatcatgcac ttcttcggcc cgccgtcgaa gcccgacaag gacgtgctcc
     1621 tgtactgggg gatcaagacg aagtcgaacg aggagctgcg ccaggagttc ttcacgacgt
     1681 acgtgccgaa gctctgggac ctcggcatct cggttcccga ccccgagctc cggttcgatg
     1741 aggagcgagg cgagtggagc tggtccgacc ccggctggga cgacttctgg caggtcgtga
     1801 gaggcgatgg gccgatgacg aagacgcgcc tgctgtaccg gaaggcgatg tgggaccacc
     1861 actcgtgggt ccgcgaggtc ttcgcgggga tcccgcacgc cgcctgagcc gatgcacatc
     1921 tgggacgtgc tttcgcgccg ccgcgacgac gagccgtggg aggccgtcgg atccgtcaac
     1981 gcgccggacc tcgacctcgc gatgatgctc gcgcgcgaga cgcacttccg tcacggcgag
     2041 ggcacgacgt acgcgctcac gcagcgcggc gacgacaaga tccatccatg tccggacccg
     2101 accgggatcg gcggcgtgat cgaccgggcg taccgtcgca gcgaggggta cgcgggcgtc
     2161 ggggcgaagc tgcggcgcgt ccacgaggcg atgcgagaac gaggcctcgt gatcgaccgg
     2221 ccgcggccgc cccgccaccg ggcccgcgcg gagatctccc atggctcctg acccgcgcct
     2281 cgagctgctg ctcgcgctcg ccgacgacga gctcgtcctc ggtcaccgcc acgccgagtg
     2341 gacggggtgg gcgccgtacg tcgaggagga cctcgcgttc tcttcgatcg cgcaagacga
     2401 gatcgcgcac gcccgcctgc tctacgagat cgcggagccg ctcgccaggc gggatcccga
     2461 cgcgctcgcg ctcggccgcg agccgaacga gtaccgcaac gcgatcctgt gcgagcggcc
     2521 gaaccgggac tggggataca cggtcgcgcg ccagtacctc tacgacaccg ccgacgacgt
     2581 gcgcctgcgt gcgctcgagg ggtcgtcgct cgcggagctc gcgcacgccg tgcgcacgat
     2641 ccgcctcgag gagcgctacc acctcgaaca cgcgcaccag tggttccggc gcatcgcgca
     2701 cgggccgtcg ctcgaggcgc gcgagcggtt cgctgcgggt cttgaggcgg cgatcgccga
     2761 ggccgtcgcg ctgttcgagg cgctcccgag cgaggaggag ctcgtcgcgg acggcgtcct
     2821 cccgcgctcg aacgaggatc tcctgggtga atggctctcg gcgcttggcg aggcgctgga
     2881 agaggcgtcg ctcgactggg tgctcacgga gcacgcgcgc caagccggcg agatggtgcc
     2941 gacgtcctcg ggagagatcg aggcgggccc gacgctcgca gtccccggcg tcgagcgtcg
     3001 cgacggccgc tgggtgcacg tcggcggctt cgccggcgcc ggcggacggc gcgggcgtcg
     3061 cagcgaggag ttccaggcgc tgtgggagga gatgacggag ctctaccggg cgctgccagg
     3121 tgcgcgatgg tgaccgagac gacgtccgtg ctcgacgacg tgcgcgccgc cgtcgcccgc
     3181 gtgcgagacc ccgagatccc ggcgggcacg atcgccgacc tcgggatgat ccacgacgtc
     3241 cgtgtcgcgg gggacgccgt cgagatcgag ctgctcccga cgttcgtcgg gtgccccgcg
     3301 aaggacgtga tcggcgagga cgtccgccgc gctgcggaac ccgtcgccga tcggccggtc
     3361 ggggttcgct tcgtctacga cccgccgtgg acgaccgacc gcatcaccga gcagggacgc
     3421 gcgagactcc gggagttcgg gatcgccccg cactgggagc gcgggcccga gcccgtcccg
     3481 atcccgctgt tgtcgcgcgc cggcgtgcag tgtccgttct gcgggtccga ggagaccgtc
     3541 atggactcgg cgtggggcgt gacgccgtgc cggtcgacgc attactgccg ctcgtgccgt
     3601 aaccccttcg agggcttcaa gacgaaaagc ctctgagcga cgcatcgcac gttgacaaaa
     3661 ccatgcctcc gcgatacgcc tggcgtaccg atccaaagga ggcgatatgg gaaggagagc
     3721 gctcctcgcg ctgctcgtcg tcgcgacgct ggtggccggt atcgcgtcgc tggcgacggc
     3781 cggtcacggc gggttccatg cgaccctcga cggctacgag gaggtcccgg cgatctccac
     3841 gacgggcgag gggacgtttc gggccgagat cgtgagcacg gcgaaaggcg acgtgatccg
     3901 gtacgagctc acgtattcgg gcctcgaggc cgatgccaca gcggcccaca tccacttcgg
     3961 ccagccggcg gtcgcgggcg gcgtgatcgc gtggctgtgt ggcggaggcg gcaagccggc
     4021 gtgtccgccg cgcgcaggaa cggtgcgcgg gacgataacg ccgggcgaca tcatcggtcc
     4081 cgagaaccag gggatcgcgc ccggcgagtt cggcgaagcg gtccgcgcga tgcgcgctgg
     4141 ggtcacgtac gcgaacgtgc actcgtcgat gttcccgacc ggcgagatcc gcggtcagat
     4201 cgggagagac ttcacgtaga cgcttttcgg gcctccgggg tccgggactt ccgcttgcgg
     4261 atgcggacga cggtccccgg atggtccttc gttcacttct tcatcgcgaa ctcgtccgag
     4321 gcagcgagca tcacggggat gccgcgcccc gtctcctcct cggggccgac catggcggga
     4381 gggcggaaaa cgccgccgtc acggatctcg acctcgacga gattcggatc ttccttccag
     4441 aagatctcta cgttgtgggc cgtgctgtgg aggaccgagt tcgtgcaggc ctcgttgacc
     4501 gcgatcacga gctcctcggt cagcgacggt tggaggtcgc ggccggacgc gagagatcgc
     4561 acccaccggc ggacgtccgc gagcgccgac gggtgagcgc ggaactcgcg ctggtcgcgg
     4621 gcgtccacgt cctcctctca ggctcgccga accccgatta gatgccccgg tcaagagcgc
     4681 cctacacgct caacgaccct cgaacttcgg ctcgcgcttc tccgcgaacg ccatgacgcc
     4741 ctccaggtgg tcgtggctct cggccatctg cgcctggagc tcgccttcgc gttcgagcgc
     4801 ctcctcgagg cccgtcgtga gcgcgccgag catcagctcg cggcttgcaa cgagcgcgcg
     4861 cgtcgggag
//
LOCUS       DASZOB010000126         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_112661, whole genome shotgun sequence.
ACCESSION   DASZOB010000126 DASZOB010000000
VERSION     DASZOB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112661"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VG709_02010"
     CDS             complement(<1..251)
                     /locus_tag="VG709_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31885.1"
                     /translation="MPLLVAAFTVARPDPLQPPRLEPSFDQTTAVAYATELARRFPDR
                     SPGSAGSREAAEWVSARLRDAELEAHRDEFTADVPAQGRT"
     gene            432..1130
                     /locus_tag="VG709_02015"
     CDS             432..1130
                     /locus_tag="VG709_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC31886.1"
                     /translation="MATGVTILLVDDEESIQKLLTYPLERDGHRVVAARDGEEALARF
                     REENVDLVLLDLALPKVDGLEVCRRIRAASSVPILMLTARDDEIDKVLGLEIGADDYI
                     TKPFSIRELRSRIRAAVRRSGLTPAVESPDVIETDGLRIDVPRRVVEVDGSPVPLTYL
                     EFELLRTLAATPGHVYTRQALLDKLWGGAAFRDPRTIDVHVRHLRAKIERDASNPEYI
                     FTVRGVGYRFRDGR"
     gene            1127..>1536
                     /locus_tag="VG709_02020"
     CDS             1127..>1536
                     /locus_tag="VG709_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31887.1"
                     /translation="MSPPAGTTARRRLLPRFRATLGVKLSLVLFGVVAGALAIVYLMV
                     VPRLESRLVDAKIRQLERPPAPLLVSHVGERAGDRVALRDAVDVASANLNARGVVFQR
                     IGEAELLLHADSSAVPEADLTEDPVALEASATLRP"
BASE COUNT          195 a          567 c          561 g          213 t
ORIGIN      
        1 gttcgcccct gcgctggaac gtcggccgtg aactcgtcgc ggtgggcctc gagctcggcg
       61 tcgcgaaggc gcgcgctcac ccactcggcc gcctcgcgcg agcccgcgga cccgggcgag
      121 cggtcgggga accggcgcgc gagctcggtc gcgtaggcga ccgccgtcgt ctggtcgaac
      181 gacggctcaa gccgcggcgg ctgcagcggg tcgggccgcg cgaccgtgaa cgcggcgacg
      241 aggagaggca cggccgcgac ggtccacgct gcgcggtaga ggcgcgtgga gaccggccgc
      301 tcgagcgtgc cgcgcctcgc gcgccgccgc tgagtcgggg cgtccggccg ggaatccggg
      361 ccgtcgcgtc gcgtgccggt cggggccaga acagcccgat cctagtccgt ggcgtcccta
      421 tcctgtcctc gatggcgacc ggcgtgacca tcctgctcgt ggacgacgag gagtcgatcc
      481 agaagctcct cacctatccg ctcgagcggg acggccatcg ggtcgtcgcg gcgcgggacg
      541 gcgaggaggc gctcgcccgc ttccgcgagg agaacgtgga cctcgtgctc ctcgatctcg
      601 cgctgccgaa ggtcgacggc ctcgaggtat gccggcgcat ccgtgccgcg agctccgtcc
      661 cgatcctcat gttgaccgcg cgcgacgacg agatcgacaa ggtgctcggc ctcgagatcg
      721 gcgccgacga ctacatcacg aagccgttct cgatcaggga gctgcgaagc cggatccggg
      781 cggccgtgcg gcgctcgggt ctcacgcccg ccgtggagtc gcccgacgtg atcgagacgg
      841 acggtctccg gatcgacgtg ccgcggcggg tggtcgaggt ggacggctcg ccggtgccgc
      901 tcacctacct cgagttcgag ctcctgcgca cgctcgccgc gacgccgggc cacgtgtaca
      961 cgcgccaggc gttgctcgac aagctgtggg gcggagcggc gttccgcgac ccgcggacga
     1021 tcgacgtcca cgtccggcac cttcgggcga agatcgagcg cgatgcgagc aaccccgaat
     1081 acatcttcac ggtgcggggc gtcgggtacc gcttccggga cggccggtga gcccgcccgc
     1141 ggggacgacc gcccggcgcc gcctgctgcc gcgcttccgg gcgacgctcg gcgtcaagct
     1201 gtccctcgtc ctcttcgggg tcgtcgccgg ggcgctcgcg atcgtctatc tgatggtcgt
     1261 cccccggctc gagtcgcgcc tcgtcgacgc gaagatccgc cagctcgagc ggccgcccgc
     1321 acccctcctc gtctcgcacg tcggggagag ggccggcgac cgcgtcgcgc tccgtgacgc
     1381 cgtcgacgtc gcgtccgcga acctgaacgc gcgcggcgtc gtcttccagc ggatcggcga
     1441 ggccgaactg ctgctccacg ccgactcgag cgccgtcccg gaggccgacc tcacggaaga
     1501 ccccgtcgcg ctcgaggcgt cggcgacgct ccggcc
//
LOCUS       DASZOB010000127         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_93574, whole genome shotgun sequence.
ACCESSION   DASZOB010000127 DASZOB010000000
VERSION     DASZOB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93574"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VG709_02025"
     CDS             <1..398
                     /locus_tag="VG709_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC31888.1"
                     /translation="VPSTAVLIFTAYGERSRLGRGLESGAKGYILKEAPHQTLLRAIE
                     KVANGDGYVDPALMPEFLTRDREDMLTPREREILQLLADGMSNADVASRLFISQETVK
                     SHVRRILSKLEADTRTQAVAIALREAIID"
     gene            415..1500
                     /locus_tag="VG709_02030"
     CDS             415..1500
                     /locus_tag="VG709_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC31889.1"
                     /translation="MSGTSGGSSGSAPSAADTAAGESSCLESLVGSLLAVSEPRDPEQ
                     TLERTRAEAQRIFHARAARFLSPEETPVAEAQVTEGGIRVPLHDGDVAIGTLELERAE
                     PFSAWELLRATTLAAFAARAAQNARLVTEAHEREQDRARLTERLVTAEQDERRRLSIF
                     LHDGPLQAMSGIALMHDAALAAIHDGRYDEAARVIASSLEHERQTIRTLRDLSFAIEP
                     VVLRAQGFTAAVRSLGEQVERSHGVTVVAEVEAGESLGEKAQVALYQLVREALDQAMR
                     RKPGRIVVAVRAEDGGLTTEIRDDGIGERRRRGVEELAERVAVLNGTVTMDAQPEGGT
                     TVRVVLPPYAGATTAGARPPERDPEPE"
     gene            1514..1702
                     /locus_tag="VG709_02035"
     CDS             1514..1702
                     /locus_tag="VG709_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31890.1"
                     /translation="MGDGRYLLFLSKTSGYELAEREGEPPAAGTRLEDREVPFFVSKV
                     AASPLPGDERPCAYLQPI"
     gene            complement(1739..>2202)
                     /locus_tag="VG709_02040"
     CDS             complement(1739..>2202)
                     /locus_tag="VG709_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31891.1"
                     /translation="SVTPDGFRSLAQFYARHARVTNAAGEEYEPHTWSEIDVVQWTAR
                     QPGARAWYAVPDDALGARVRGRTVAEMVEAARAAGAPVERRDGETVVEVVAGITTTLG
                     GLRVDERARAAEGMYVCGADVGGISTGGYQSSLAAARVVGRIAAEQAVAAA"
BASE COUNT          321 a          826 c          778 g          277 t
ORIGIN      
        1 aggtgccgtc gacggccgtg ctcatcttca cggcctacgg cgagcggagc cgcctcgggc
       61 gcggcctcga gtccggcgcg aagggataca tcctgaagga ggcaccccac cagacgctcc
      121 tccgcgccat cgagaaggtc gcgaacggcg acggatacgt ggatcccgct ctaatgcccg
      181 agttcctcac gcgcgaccgc gaggacatgc tgacgccgcg cgagcgcgag atcctgcagc
      241 tcctcgccga cggcatgtcg aacgccgacg tggcctcccg gctcttcatc agccaggaga
      301 cggtcaagag ccacgtgcgc cgcatcctct cgaagctcga ggcggacacc cgaacccaag
      361 ctgtcgcgat cgcgcttcgc gaagcgatca tcgactagac aacccgcccg acgattgagc
      421 ggaacctcgg gcggatcctc cggctcggcg ccttcggcgg cggacacggc cgccggggag
      481 tcgtcgtgcc tggagtcgct cgtcgggtcg ctgctcgccg tctcggagcc acgcgatccg
      541 gagcagacgc tcgagcggac gcgcgcggag gcgcagagga tcttccacgc acgcgctgcg
      601 aggttcctct cgcccgagga gacgcctgtc gccgaggccc aggtaaccga gggcggcatc
      661 cgggttccgc tccacgacgg cgacgtcgcg atcggcacgc tcgagctcga acgggcggag
      721 ccgttcagcg cctgggaact cttgcgggcg actacgctcg cggcgtttgc ggcgcgggcg
      781 gcgcaaaacg cacgtctcgt aacggaggcg cacgaacgag agcaggaccg cgcccgcctg
      841 acggagcggc tggtcacggc ggagcaggac gagcggcgcc gcctgtcgat ctttctccac
      901 gacggccctc tccaggcgat gtccggaatc gcgctcatgc acgacgccgc gctcgccgcc
      961 atccacgacg gtcgctacga cgaggccgcg cgggtgatcg cgagctcgct cgagcacgag
     1021 cgccagacga tccggacgct ccgcgacctc tccttcgcga tcgagcccgt cgtcctacgc
     1081 gcccagggct tcacggcggc cgtccgctcg ctcggggagc aggtggagcg ctcgcacggc
     1141 gtcactgtcg tcgccgaggt cgaggcaggc gagtccctgg gcgagaaggc gcaggtcgcg
     1201 ctctaccagc ttgtacgcga ggcgctcgac caggccatgc gcaggaagcc cggccggatc
     1261 gtcgtggccg tgcgcgccga ggacggcggg ctcacgaccg agatccggga cgacggcatc
     1321 ggcgagcggc gtcgccgggg cgtcgaggag ctcgccgagc gagtcgcggt cctgaacggg
     1381 accgtcacga tggacgcgca gcccgagggc gggacgacgg tccgcgtcgt cctccctccc
     1441 tacgcaggcg ccacgactgc gggcgcgcgg ccgcctgaac gcgaccccga gccggagtag
     1501 aatcgggccg atggtgggcg acggccgcta cctcctcttc ctctcgaaga cgtccgggta
     1561 cgagctcgcc gagcgcgagg gcgagccgcc ggccgcgggc acccgcctcg aggaccgcga
     1621 ggtgccgttc ttcgtctcca aggtcgccgc gtcgcctctc ccgggcgacg agcgcccctg
     1681 cgcgtacctc cagccgatct aggcgaatac gcgcgacgag gcgcgaccgg cgcggccgtt
     1741 acgccgcggc cacggcttgc tcggcggcga tgcggccgac gacgcgggcg gccgcgaggc
     1801 tgctctggta gccgcccgtc gagatgccgc cgacgtccgc gccgcacacg tacatcccct
     1861 ccgcggcccg cgcccgctcg tcgacgcgca ggccgccgag cgtcgtcgtg atcccggcga
     1921 cgacctcgac gaccgtctcg ccgtcccgcc gctccaccgg cgcacccgcg gcccgcgccg
     1981 cctcgaccat ctcggcgacg gttcgaccgc ggacgcgcgc gccgagcgcg tcgtcgggca
     2041 ccgcatacca ggcgcgcgcc cccggctgcc gcgccgtcca ctggacgacg tcgatctcgg
     2101 accacgtgtg cggctcgtac tcctcgccgg ccgcgttcgt cacacgcgca tggcgcgcgt
     2161 agaactgcgc gagcgagcgg aagccgtcgg gcgtgacaga gg
//
LOCUS       DASZOB010000128         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_93580, whole genome shotgun sequence.
ACCESSION   DASZOB010000128 DASZOB010000000
VERSION     DASZOB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93580"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3416
                     /locus_tag="VG709_02045"
     CDS             <1..3416
                     /locus_tag="VG709_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWC31892.1"
                     /translation="LPMRQTTERDPEDGTGRHRGQRAARPAEDVVQRFYATFDDAPVA
                     LYRTTPAGQLLNANRALVELLGHPDRASLMSSNMSELYVDRDDRRRWKEMVARDDALK
                     GHEWRLRRRDGSMIWVRDTARAVRDEDGHIRFYDGAIVDVTERRLAEQALRESEARYR
                     RLVERLPAIVYSAELDESGRWRYVSPQIESMLGFAAETWVRENLWRERLHPADRDRVL
                     AEEARSRDRGEAFSSEYRLLARDGREVWVRDEATVHRPGRDGRALLEGLIHDITPQKL
                     AAAALADRNAELEALHEMTVGLIRELDPTEVLRMLVSRAAMLLGTEHGYVYTVDDAGG
                     ADEMVCGVGIGLFESVVGVRLRRGEGVAGRVWDRAQPLSIEDYSSWDGRSQAFDHLEI
                     GAVVGVPLLSGDTVVGVLGVVRLDERRGFSADDLELLQRFARFGSLALHNARLYTALR
                     NELSGRERVEGSLRRRNRELAALSETTAGLIAGLDPERVLRAVVERATELMGTKHGWL
                     SVLDEERGEIEVRVGQGVFAGWVGQRQQRGVGLDGEVWDQQRSITVEDYATWRHRMRQ
                     FDRSAIGPVAAVPLRARSDMAGVIGVGRLEKERTFTVEELELLERLAYVASLALHGAR
                     LYRRLELELSERRRAEAVLLEREQQLNEAEAVAHIGSWTWEVGADRVVWSRELYDIFG
                     VDAERYRPTYDGFVDLVHPDDRDAVAIDIARALDEGGSFARDHRIVRPDGSERWLHGR
                     GDVIVGEEGEPVRMRGTAQDITERKLAEEARRKSEELFRELLESAPDAVVIIDADGRI
                     VLVNAQTEALFGYDRGEIVGRDVEVLVPERLRDGHPRHRGRYAADPKTRPMGTGLELL
                     GRRRDGTEFPVDITLSGLTTDGETTVMAIVRDVTDQRKAQEALREAYEREREAAERLR
                     MIDEMKNSFLEAVSHDLRTPLTSVLGYAVTLQRPDLDVPDEQRREMLDLLVANARKLE
                     RLLSDLLDLDRLLRGVVEPHRRPTDLADLLRHVIAEADVQGRPLEIVAEPVVLAVDPA
                     KIERIIENLLVNAVRHTPEGTRLWVRISAMRGGALIAVEDEGRGVPASEREEIFDPFR
                     RGSRSPAHAPGSGIGLSLVGRFTELHGGRAWVEDRPGGGASFKVFLPAV"
BASE COUNT          510 a         1226 c         1329 g          492 t
ORIGIN      
        1 acctccccat gcggcagaca accgaacgag accccgagga cggcaccggc cgccaccgcg
       61 gccagcgggc cgcgcgaccg gccgaggacg tggtccagcg cttctacgcg acgttcgacg
      121 acgcgccggt cgcgctctac cgcacgacgc cggcaggcca gctgctcaac gcgaaccgag
      181 cgctcgtcga gctcctcggg caccccgacc gcgcgtcgct catgtcctcg aacatgtcgg
      241 agctctacgt cgaccgggac gaccggcggc ggtggaagga gatggtggcg cgagacgacg
      301 cgctcaaggg gcacgaatgg cgccttcgcc ggcgggacgg gtcgatgatc tgggtccgcg
      361 acaccgcacg cgccgtccgg gacgaggacg gccacatccg tttctacgac ggcgcgatcg
      421 tcgacgtcac cgagcggcgc ctcgccgagc aagcgcttcg cgagtccgag gctcgttacc
      481 ggcggctcgt cgagcgcctc ccggcgatcg tgtacagcgc cgagctcgac gagtccgggc
      541 ggtggcgcta cgtgagcccg cagatcgaat cgatgctcgg cttcgccgcc gagacgtggg
      601 tgcgcgagaa cctgtggcgg gaacggctcc acccagccga tcgcgatcgc gtcctcgccg
      661 aggaggcgcg ttctcgtgac cgaggagagg cgttctcgtc ggagtaccgg ctgctcgctc
      721 gcgacggccg tgaggtatgg gtccgcgacg aggcgaccgt ccaccgtccg gggcgtgacg
      781 gacgcgccct gctggaaggt ctgatccacg acatcacgcc gcagaagctt gcggccgcgg
      841 cgctggcgga ccggaacgcc gagctcgagg cgctccacga gatgaccgtg ggtctgatcc
      901 gcgagctgga cccgacggag gtgctccgca tgctcgtgtc gcgcgcagcg atgctgctag
      961 gcaccgagca tgggtacgtc tacacggtcg acgacgccgg cggcgccgac gagatggtct
     1021 gcggcgtggg gatcggcctc ttcgagtccg tcgtcggcgt tcgcctgcgc cgcggcgagg
     1081 gcgtcgccgg gcgcgtctgg gatcgcgccc agccgctgtc gatcgaggac tactcgtcgt
     1141 gggacgggcg gagccaagcg ttcgaccacc tcgagatcgg agccgtcgtc ggggtcccgc
     1201 tgctctcggg cgacacggtc gtgggggtgc tcggcgtcgt ccgcctcgac gagcgccgcg
     1261 ggttctccgc tgacgacctc gagctgctcc agcggttcgc gcggttcgga tcgctcgcgc
     1321 tccacaacgc acggctgtac acggcgctgc gcaacgagct ctcggggcgc gagcgggtgg
     1381 agggatcgct gcgccggcgg aaccgcgagc tggccgcgct gtcggagacc accgccggcc
     1441 tgatcgcggg cctcgatccc gaacgggtcc tgcgcgccgt cgtcgagcgc gcgaccgagc
     1501 tcatggggac gaagcacggc tggctctcgg tcctcgacga ggaacggggc gagatcgagg
     1561 tgcgggtcgg ccagggcgtc ttcgccggct gggtcggcca acgccagcag cgcggcgtcg
     1621 ggctcgacgg cgaggtgtgg gaccagcagc ggtcgatcac ggtcgaggac tacgcgacgt
     1681 ggcgccaccg gatgcggcag ttcgaccgaa gcgcgatagg ccccgtggca gccgtgccgc
     1741 tccgcgcgcg ctcggatatg gcgggggtca tcggcgtcgg ccgtctcgag aaggagcgga
     1801 cgttcacggt ggaggagctc gagctcctcg agcggctcgc ctacgtggcg tccctggcgc
     1861 ttcacggcgc gaggctctac cggcgcctgg agctcgagct ctcggagcgg cggcgggccg
     1921 aggcggtgct cctcgagcgc gagcagcagc tgaacgaggc cgaggccgtc gcgcacatcg
     1981 gctcgtggac gtgggaggtc ggcgccgatc gcgtggtgtg gtcgcgcgag ctctacgaca
     2041 tcttcggggt ggacgcggaa cgataccgcc cgacgtacga cgggttcgta gacctcgtcc
     2101 acccggacga ccgcgacgcg gtggcgatcg acatcgcgcg ggcgctggac gaggggggct
     2161 cgttcgctcg ggaccaccgg atcgtccggc cggacggcag cgagcgatgg ctccacggcc
     2221 ggggcgacgt gatcgtcggc gaggaaggcg agcccgtccg gatgcgcgga accgctcagg
     2281 acatcaccga gcggaagctc gccgaggagg cgaggcgcaa gagcgaggag ctgttccgcg
     2341 agctgctcga atcggcgccg gacgccgtcg tgatcatcga cgccgacggg cggatcgtcc
     2401 tcgtgaacgc gcagaccgag gcgctgttcg ggtacgaccg cggcgagatc gtgggtcgcg
     2461 acgtcgaggt gctggtcccc gagcgcctcc gcgacggcca cccgcgccac cgcgggcggt
     2521 acgcggccga tccgaagacg aggccgatgg ggacggggct cgaactcctc ggacggcgtc
     2581 gcgacggcac ggagttcccg gtcgatatca cgctctcggg tctcacgacc gacggcgaga
     2641 ccaccgtgat ggcgatcgtg cgcgacgtca ccgaccagcg gaaggcccag gaggcgctcc
     2701 gcgaggccta cgagcgggaa cgcgaggcgg cggagcgtct gcggatgatc gacgagatga
     2761 agaacagctt cttggaggcg gtgtcccacg acctccgcac gcccctcacg tcggtcctcg
     2821 ggtacgccgt gacgctccag cgtccggacc tcgatgttcc cgacgagcag cgccgcgaga
     2881 tgctcgacct cctcgtcgcg aacgcgcgca agctcgaacg gttgctctcg gacctgctcg
     2941 acctcgacag gttgctccgc ggcgtcgtcg agccgcaccg gcggcccacg gatctcgccg
     3001 acctcctccg ccacgtgatc gccgaagccg acgtccaggg gcggccgctc gagatcgtcg
     3061 ccgagcctgt cgttctcgcc gtcgaccccg cgaagatcga gcggatcatc gagaatttgc
     3121 tcgtcaacgc cgtccggcac acgcccgagg gcacgcggct ctgggtgagg atcagcgcga
     3181 tgcgaggcgg cgcgctgatc gcggtcgagg acgagggacg cggcgttccg gcctccgagc
     3241 gcgaggagat attcgacccg ttccgccgag ggtctcgctc gcccgcgcac gcccccggaa
     3301 gcgggatcgg cctgtcgctc gtcggacgct tcaccgagct ccacggcggt cgcgcatggg
     3361 tggaggaccg cccgggcggc ggggcctcgt tcaaggtttt cctcccggcg gtctgacctc
     3421 ggtcgcgtcg gcgaccccgt cgtacgatgc cccgcgtgat gaaccgcgcc gagcgcatgg
     3481 aggtcgcgcg ccggctgcgg cgccgatcgt tcgcgctcgc cgtcgtggtc ccgctcgtcg
     3541 tcgtggccgc cgggctc
//
LOCUS       DASZOB010000129         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_73305, whole genome shotgun sequence.
ACCESSION   DASZOB010000129 DASZOB010000000
VERSION     DASZOB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73305"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..708)
                     /locus_tag="VG709_02050"
     CDS             complement(13..708)
                     /locus_tag="VG709_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWC31893.1"
                     /translation="MTPAIEAADLRFGFGGEPVLDGVDLTVEPGTFVGLTGPNGAGKS
                     TLLKLSVGLLRPSSGTLRVLGADPLDAAVRRKVGYASQGSPTRVVLPISVSEVVEAGM
                     TRGRGVVRRPRVSERALVERAIDAVGLGKLAAECVFELSGGQQQRAAIARALVGDPPL
                     LLLDEPTTGIDRAFRPQLVEDLRRRADGGAAVVVVSHDPDDFHDETDRILFLEDGRLR
                     DVAHEEYHGASQR"
     gene            complement(705..1562)
                     /locus_tag="VG709_02055"
     CDS             complement(705..1562)
                     /locus_tag="VG709_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWC31894.1"
                     /translation="MRMRLMGAAVAAALLAACSFGLGERVIVATVYPVAYAAERIAGP
                     EWDVIDLTAGAAEAHDLELSLEQRAAIEGADVVFYYGDLGFQPHVEEAVRDAQGTVVA
                     INGPNAADAQADPHLWLWPNLYLSRIADHVQIAMWDIDEARAEEYERRARTLFRDLER
                     LQRRYDDVLGGPCEYRTAIVSHEAFGYLLERRYEFRQFGLAGLSPEGEPTSGRIEGAQ
                     ELIEDRKAGAVFYEPTDEARRVAERVADDGGVPALPLSPLETRPAEGDYLSVMEDNLR
                     SLRRGLSCR"
     gene            1614..>2270
                     /locus_tag="VG709_02060"
     CDS             1614..>2270
                     /locus_tag="VG709_02060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWC31895.1"
                     /translation="MDDGLIGSRIRSVLLVALVFALAGGIGYGIGRLFGRTGLVGAAV
                     ALGIAAVTALWSYFFGDRLVLRASRAVPAHPREYAVLHNVVEGLCAQAGLPKPALYVI
                     PEAAPNAFATGRDPAHAKIAVTAGLLESMNRVELEGVLAHELSHVAGRDVLLGTLVAT
                     LVGAIVLLFGWARNVLSWGAAQARAGRGIVSLLAWVVLSIGALLSFLAPLVAQILRLA
                     I"
BASE COUNT          322 a          809 c          784 g          355 t
ORIGIN      
        1 agagcaggtc gatcatcgct gcgacgctcc gtgatattcc tcgtgcgcga cgtcgcgcag
       61 gcgtccgtct tcgaggaaca ggatccggtc ggtctcgtcg tggaagtcgt cggggtcgtg
      121 ggaaacgacc acgacggcgg cgcccccgtc ggcgcggcga cgaaggtcct cgacgagctg
      181 cgggcggaac gcgcggtcga tccccgtggt cggctcgtcg agcagcagca gcggggggtc
      241 accgacgagg gctcgcgcga tcgccgcgcg ctgctgctgc ccgcccgaca gctcgaagac
      301 gcactcggcc gccagcttcc cgagccctac cgcgtcgatg gcgcgctcga cgagggcccg
      361 ctccgacacg cgcggccggc gcacgacgcc tcgcccgcgt gtcatgccgg cctctacgac
      421 ctccgacacc gagatcggaa ggacgacacg ggtcggcgat ccctgcgacg cgtacccgac
      481 ctttctccgc accgcagcgt cgagcggatc cgctccgagg acgcgcaacg tccccgacga
      541 cggtcggagc agccccacgg agagcttcag cagcgtcgac ttccccgccc cgttcgggcc
      601 ggtcaaccca acgaaggtcc cgggctcgac cgtgaggtcg acgccgtcga gcacgggctc
      661 gccgccgaat ccgaaccgga gatccgccgc ctcgatcgcc ggcgtcatcg gcaactgaga
      721 cctctcctca gcgatcgcag gttgtcctcc atcaccgaga ggtagtcgcc ctcggccggc
      781 cgcgtctcga gaggagagag cggaagcgcc ggaaccccgc cgtcgtcggc gacgcgctcc
      841 gcgacgcgtc tggcttcgtc ggtgggttcg tagaagaccg cgcccgcctt ccgatcttcg
      901 atcagctcct gcgcaccttc gatgcgcccg ctggtcggtt cgccttcggg gctcaggccg
      961 gccaacccga actgccggaa ctcgtagcga cgttcgagga gatacccaaa cgcctcgtgg
     1021 gaaacgatcg ccgtccggta ctcgcacggc ccgcccaaga cgtcgtcgta gcgccgctgc
     1081 agccgctcga ggtcgcgaaa cagtgtgcgc gctcgccgct cgtactcttc cgctcgcgcc
     1141 tcgtcgatgt cccacatcgc gatctgcacg tgatccgcga tgcgtgagag atagaggttc
     1201 ggccacagcc agagatgtgg gtccgcctgc gcgtccgccg cgttcgggcc gttgatcgcg
     1261 acgacggttc cctgagcatc gcgaaccgcc tcctcgacgt gcggctggaa gccgaggtcg
     1321 ccgtagtaga agacgacgtc ggcgccttcg atggcggccc gctgctcgag cgagagctcg
     1381 aggtcgtgcg cctccgcggc tccggcggtc aggtcgatga cgtcccactc cggcccggcg
     1441 atgcgctccg ccgcgtacgc gacgggatag accgtcgcga cgatcacccg ctcgccgagg
     1501 ccgaacgagc aggcggcgag gagcgcggcc gccaccgcag cgcccatgag ccgcatccgc
     1561 atggtgagaa tgataatcaa tctcaagcga ccgtgcttgc tacgatcgcc gcgatggacg
     1621 acggccttat cgggagtcgc atccgatccg tcctcttggt ggcgttggtg ttcgcgctcg
     1681 ccggagggat cgggtacggc atcggacgtc tcttcgggag gacggggctg gtcggcgccg
     1741 ccgtcgcgct cgggatcgcc gccgtcacgg cgctgtggtc gtacttcttc ggagaccggc
     1801 tcgtcctccg cgcaagcaga gccgtgccgg cgcacccgcg cgagtacgcg gtcctccaca
     1861 acgttgtcga ggggctgtgc gcccaggccg gtctgccgaa gccggcgctc tacgtgatcc
     1921 ccgaggcggc cccgaacgcg ttcgcgaccg ggagggatcc agcgcatgcc aagatcgcgg
     1981 tgaccgcggg gctgctcgag tcgatgaacc gcgtcgagct cgagggcgtg ctcgcgcacg
     2041 agctctcgca cgtcgccggg cgcgacgtgc tgctcgggac gctcgtcgcg acgctcgtcg
     2101 gagcgatcgt gctcctgttc gggtgggcgc ggaacgttct ctcgtggggc gccgcgcagg
     2161 ccagggcggg tcgagggatc gtgtcgttgc tcgcctgggt cgtgctctcg atcggcgcgt
     2221 tgctgtcgtt cctcgcgccg ctcgtcgcgc agatcctgcg gctcgcgatc
//
LOCUS       DASZOB010000130         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_73352, whole genome shotgun sequence.
ACCESSION   DASZOB010000130 DASZOB010000000
VERSION     DASZOB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73352"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VG709_02065"
     CDS             complement(<1..319)
                     /locus_tag="VG709_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HWC31896.1"
                     /translation="MATRAPALDQEVGPRAAGAPADALDAAERWVEIEGQRYRRRNPM
                     TEYLLVALTLGIWALVWHYRTNDDARRFLRDESIRPALSAMAFMTGMAAPVPLVVSIV
                     LAIV"
     gene            complement(325..1314)
                     /gene="nuoH"
                     /locus_tag="VG709_02070"
     CDS             complement(325..1314)
                     /gene="nuoH"
                     /locus_tag="VG709_02070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016814944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWC31897.1"
                     /translation="MSFVDQLFSNVWIVLGLKVVLVMGFFLTAPLGVGYAEHKILAHM
                     QHRLGPMEAGRFHGWAQLIADGVKFVQKEDVIPRAADRWVFSLAPGVVLVPVILAVIV
                     LPFGPTVWAENLDVGLFFLMAISSVSVIGVLMAGWASANKYSLIGAVRAAAQLIAYEL
                     PLILAAVSIAMLGGTLSLVGIVEAQQGIWFVFLQPIPFLIFVMASLAELIRTPFDMPI
                     ADSEIIFGAYTEYTGLKFAFFLLSEYAGIVVLSAVASVLFLGGYLAPPLLGFIPAPLW
                     MMAKIGGLSFVIIWLRATYPRLREDQLQRFSWIVLIPLALLNILVTAVVKVLV"
     gene            complement(1315..1842)
                     /locus_tag="VG709_02075"
     CDS             complement(1315..1842)
                     /locus_tag="VG709_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWC31898.1"
                     /translation="MASSPTPLPAAEITRRLRARFGDAVEVAEMTGQAVVRVGRDRYR
                     ELVAALRDDRDLAFDFPDFVSAVDRGDEGLEVVTQLYSTRHRQTVRVAVPCDGDEPTC
                     PSISDLYAGMNWHERETWELFGIRFDGHPSLVKLLLPEQFEGYPLRKDFPLTTRVAKP
                     WPGEEEGEAIEEVDT"
     gene            complement(1808..2320)
                     /locus_tag="VG709_02080"
     CDS             complement(1808..2320)
                     /locus_tag="VG709_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWC31899.1"
                     /translation="MGLIERTALPNIQPIKFLLNWGRRYSLWVMNFGLACCAIEFIAA
                     STAKHDFIRFGVIPFAHGPRQADLLIVAGTVTDKMAPALKRVYEQMPDPKYVISMGSC
                     SNIGGPYWDSYSVTKGVDQIIPVDVYVPGCPPRPEALLQGIIKLQEKIGSEDIREKWS
                     GKLTDAPAGS"
     gene            complement(2311..2676)
                     /gene="ndhC"
                     /locus_tag="VG709_02085"
     CDS             complement(2311..2676)
                     /gene="ndhC"
                     /locus_tag="VG709_02085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWC31900.1"
                     /translation="MADYFRSYGTLVALAFAGAGLVAVAFLAAKVVAPRRPLPEKLTT
                     YECGIDPVGEGWSQSQIRYYIFAFLFVIFDVESVFLFPWAVVFERLGMFAVVEMVIFI
                     VILGTGLLYAWRKGVLRWA"
     gene            2789..>3059
                     /locus_tag="VG709_02090"
     CDS             2789..>3059
                     /locus_tag="VG709_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31901.1"
                     /translation="MRRSWVAVAVVAALSAWAPPSAASRTPPRTFLLSDGVAEDGAVV
                     LFEIQPERHDLRLGHSVAWTNAGILPHELEERDELFRWGPIAPGDT"
BASE COUNT          556 a         1047 c         1037 g          419 t
ORIGIN      
        1 cgacgatcgc gagcacgatc gagaccacga gcgggaccgg ggctgccatc ccggtcatga
       61 acgccatcgc cgagagcgcc ggccggatgc tctcgtcgcg gaggaagcgg cgcgcgtcgt
      121 cgttcgtgcg gtaatgccag acgagcgccc agatcccgag cgtcagcgcg acgaggaggt
      181 actcggtcat ggggttgcgg cgccggtacc gctgcccctc gatctcgacc catcgctcgg
      241 cggcgtcgag cgcgtcggcc ggcgcgccgg cggcgcgggg gccgacctcc tggtcgagcg
      301 cgggcgcgcg tgtcgccatc gccatcagac gaggaccttc acgacggcgg tgacgaggat
      361 gttcaggagc gcgagcggga tcagcacgat ccacgagaac cgctggagct ggtcctcgcg
      421 gagacgcggg tacgtcgcgc gcagccagat gatcacgaac gagagcccgc cgatcttcgc
      481 catcatccac aggggagccg ggatgaaccc cagcagcggc ggcgcgaggt accccccgag
      541 gaacagcacc gaggcgaccg cggacaggac gacgatcccc gcgtactcag agagcaggaa
      601 gaacgcgaac ttcaaccccg tgtactccgt gtaggcgccg aagatgatct cggagtccgc
      661 gatgggcatg tcgaacggcg tccggatcag ctccgcgagc gacgccatga cgaagatgag
      721 gaacgggatc ggctggagga acacgaacca gatcccctgt tgcgcctcga cgatccccac
      781 gagcgagagc gttccgccca gcatcgcgat cgacacggcg gcgaggatca gcggcagctc
      841 gtaagcgatg agctgagcgg cggcgcggac ggcgccgatc aacgagtact tgttcgccga
      901 ggcccagccg gccatcagca cgccgatcac cgagaccgac gagatcgcca tcaggaagaa
      961 gaggccgacg tcgaggttct cggcccacac cgtcgggccg aacggcagga cgatgaccgc
     1021 gaggatcacc ggcacgagca cgacgcccgg cgcgagcgag aacacccagc ggtcggccgc
     1081 acgcgggatc acgtcctcct tctgcacgaa cttgacgccg tccgcgatga gctgcgccca
     1141 gccgtggaac cggccggcct ccatagggcc gagccggtgt tgcatgtgcg cgaggatctt
     1201 gtgctcggcg tacccgacgc cgagcggcgc cgtcaggaag aaccccatga ccaggacgac
     1261 cttgagcccg agcacgatcc agacgttcga gaaaagctgg tcgacgaacg acatctaggt
     1321 gtcgacctcc tcgatcgcct cgccctcctc ctcgccgggc cagggcttcg cgacccgggt
     1381 ggtcagcggg aaatccttac ggagcgggta tccctcgaac tgctcgggca ggaggagctt
     1441 cacgagcgac gggtgtccgt cgaagcggat gccgaacagc tcccacgtct cgcgctcgtg
     1501 ccagttcatc cccgcgtaga ggtcggagat cgacgggcag gtcggctcgt cgccgtcgca
     1561 ggggaccgcg acccgaaccg tctgacggtg gcgcgtcgag tagagctgcg tcacgacctc
     1621 gagcccctcg tctccgcggt cgacggcgga gacgaaatcc gggaagtcga acgcgaggtc
     1681 gcggtcgtcg cgcagcgcgg cgacgagctc ccggtaccgg tcccgaccga cgcggacgac
     1741 ggcctgaccc gtcatctcgg cgacctcgac cgcgtcgccg aagcgggcgc gaagcctgcg
     1801 agtgatctca gctgccggca ggggcgtcgg tgagcttgcc actccacttc tcccggatgt
     1861 cctcggatcc gatcttctcc tggagcttga tgatcccctg gaggagcgcc tccggcctcg
     1921 gagggcaccc gggaacgtag acgtcgaccg ggatgatctg gtcgacgccc ttcgtcaccg
     1981 agtaggagtc ccagtagggg ccaccgatgt tggagcacga gcccatcgag atgacgtact
     2041 tgggatcggg catctgctcg tagacccgct tgagcgccgg cgccatcttg tcggtcacgg
     2101 tccccgcgac gatgagcagg tcggcctgcc gcggcccgtg cgcgaacggg atcacgccga
     2161 agcggatgaa gtcgtgcttg gccgtcgagg cggcgatgaa ctcgatcgcg cagcacgcca
     2221 ggccgaagtt catgacccac aggctgtacc tgcgccccca gttcagcagg aacttgatcg
     2281 gctggatgtt cgggagcgcg gtccgctcga tcaggcccat ctcagcaccc ccttgcgcca
     2341 ggcatagagg agcccggtgc ccaggatcac gatgaagatc accatctcga cgaccgcgaa
     2401 catcccgagg cgctcgaaga ccacggccca ggggaacagg aagacgctct cgacgtcgaa
     2461 gatcacgaac aggaacgcga agatgtagta cctgatctgg ctctgcgacc acccctcgcc
     2521 gacgggatcg atcccgcact cgtacgtggt gagcttctcc gggagggggc gtctcggcgc
     2581 gacgaccttg gctgcgagga aggcgaccgc gaccagaccg gcccccgcga aggccagcgc
     2641 gacgagcgtc ccatacgagc ggaaatagtc ggccacgctt gtgaaagttt tcccaagcgg
     2701 attctagcag cgggccctcc cggccctgaa actcgcccgt cggcagggac ggcgccgccg
     2761 acgccgaatc tccgagcgag gagggagcat gaggcgttcc tgggtcgccg tcgccgtcgt
     2821 cgccgctctg tccgcctggg ctccgccttc ggcggccagc cgcaccccac cccggacatt
     2881 cctgctctcc gacggcgtag ccgaggacgg cgccgtcgtc ctgttcgaga tccagcccga
     2941 gcgacacgac ctccgcctcg gccacagcgt cgcctggacc aacgccggga tcctccccca
     3001 cgagctcgag gagcgggacg agctgttccg gtgggggccg atcgcgcccg gcgacaccg
//
LOCUS       DASZOB010000131         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_63613, whole genome shotgun sequence.
ACCESSION   DASZOB010000131 DASZOB010000000
VERSION     DASZOB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63613"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..451
                     /locus_tag="VG709_02095"
     CDS             20..451
                     /locus_tag="VG709_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWC31902.1"
                     /translation="MANPSMEELKKIYEEAETIAVVGASGDESKAAHTIPRYLKRQGY
                     RIFPVNPKGGEILDEKVYTSLDEIPEKVDVVDVFRPSEETPDVARAAVKAGAKVLWLQ
                     QGIFSEEAERIAREGGMEVVMNRCMGETHYQLGLGPGPHGR"
     gene            451..>1645
                     /locus_tag="VG709_02100"
     CDS             451..>1645
                     /locus_tag="VG709_02100"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC31903.1"
                     /translation="MDDLVTREQLAARSGATDEEIDALVEGGILDRTRDGFTVGDVRR
                     MKLVHACLAAGLPLEGIIQAVRDGKLSFGFLDLPSFRWGARTEATYADVATADGVPVD
                     LFLGVVEALGFPRPSPEEPAHEDDLPLLPAARAALGTGLTPEAALRVMRVWGEGLRKI
                     AEAEQWLFHNYIEMPLLRAGRSEQEMRDISVAFGEAVVPVLDRAILAVYHRQQEHAWI
                     RDLVEHVENALEEAGIHHQADVPPAMCFLDVTGYTRLTEERGDAAAADLAANLTGLVH
                     QESQRHGGRPVKWLGDGVMIFFSHPEQAVTASLDLAERIPNAGLPPVHVGIDAGPIIR
                     QEGDYYGRTVNVAARIGAYAGPHQVLVSDRVVEIASAPEFREIGAVDLKGLTRPVNLY
                     EALRPA"
BASE COUNT          261 a          574 c          582 g          228 t
ORIGIN      
        1 tccctgagga ggtagagaga tggcgaaccc gtcgatggag gagctcaaga agatctacga
       61 ggaggccgag acgatcgccg tcgtcggcgc ctcgggcgac gagtcgaagg ccgcgcacac
      121 gatcccgcgg tacctgaaac gacaggggta ccgcatcttc ccggtcaacc cgaagggcgg
      181 cgagatcctc gacgagaagg tctacacctc tctcgacgag atccccgaga aggtcgacgt
      241 cgtcgacgtc ttccgtccgt cggaggagac gcccgacgtc gcacgcgctg ccgtgaaggc
      301 aggcgcgaag gtgctctggc tgcagcaggg gatcttctcc gaggaggcgg aacgcatcgc
      361 gcgcgagggc ggcatggagg tcgtcatgaa ccgctgcatg ggcgagacgc actaccagct
      421 cgggctcggc ccggggccgc acggccgcta gtggacgacc tcgtcacccg cgagcagctc
      481 gcggctcggt ccggcgcgac cgacgaggag atcgacgcgc tcgtcgaggg cggcatcctc
      541 gaccggaccc gagacggctt cacggtcggc gacgtccgaa ggatgaagct ggtccacgcc
      601 tgcctcgcgg cggggcttcc cctggaggga atcatccagg cggtccgcga cgggaagctg
      661 tcgttcgggt tcctcgacct accctcgttc aggtgggggg cgcgcacgga ggcgacgtac
      721 gccgacgtcg cgacggcaga cggcgtcccc gtggacctct tcctcggcgt cgtcgaggcg
      781 ctcggctttc cccggccgtc gccggaggag ccggcccacg aggacgatct gccgctgctg
      841 cccgccgcac gagcggcgct cggcaccggt ctgactcccg aggcggcgct ccgcgtgatg
      901 cgcgtctggg gcgaggggct gcgcaagatc gccgaggccg agcagtggct cttccacaac
      961 tacatcgaga tgccgctcct tcgggcggga cgcagcgagc aggagatgcg cgacatctcc
     1021 gtggcgttcg gcgaggccgt cgtcccggtg ctcgaccgcg cgatcctcgc cgtctaccac
     1081 cgtcagcagg agcacgcgtg gatccgcgat ctcgtcgagc acgtcgagaa cgcgctcgag
     1141 gaggctggga tccatcacca ggcggacgtc ccccccgcga tgtgcttcct cgacgtgacc
     1201 gggtacacgc gcctgacgga ggagcgcggc gacgcggcgg cggcggacct cgcggcgaat
     1261 ctcaccggcc tcgtccacca ggagtcccag cgccacggcg gccgaccggt caagtggctg
     1321 ggcgacggcg tgatgatctt cttctcgcac cccgagcagg ccgtcacggc gtcgctcgac
     1381 ctcgccgagc ggatcccgaa cgccgggctc cctcccgtcc acgtcgggat cgacgcgggc
     1441 ccgatcatcc gccaggaggg cgactactac gggcggaccg tgaacgtcgc cgcgcgcatc
     1501 ggggcatacg cgggccctca ccaagtgctc gtgagcgacc gtgtcgtcga gatcgcctcc
     1561 gcgccggagt tccgggagat cggcgccgtc gacctcaagg gcctcacgag gcccgtcaac
     1621 ctgtacgaag cgcttcgccc cgcgt
//
LOCUS       DASZOB010000132         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_143941, whole genome shotgun sequence.
ACCESSION   DASZOB010000132 DASZOB010000000
VERSION     DASZOB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143941"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VG709_02105"
     CDS             <1..1235
                     /locus_tag="VG709_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HWC31904.1"
                     /translation="PALPHPVEAVIFGLAIVGAAFLLSWAAEAAQVDISAALAIAVLA
                     LIAVLPEYAVDFVFAFKGGKSVQAFGESCPALGGGAESPCSLALANMTGANRLLIGVG
                     WSMVAFIAWYRYRRGVRPRPSDERNTTASGIILDRQHSIELGYLAVATLYSLTLPLRE
                     SITLVDAAILVSIFAAYTWRVARAPAEDPHLVGPARYVGGRTKATRRAMVAAMFLYAA
                     AMILLTAELFAEALVATGIELGISEFFLVQWLAPLASETPELLVASLFAWRLATNAGL
                     GTLVSSKVNQWTLLVGTLPVVFTIAVGELHGLPIDSRQRQEIFLTAAQSFFAVAILSN
                     LSMSVREAWMLFGLFWAQFILGAFIPEEFHGTELVIVSVIYLLLGMIVLVRDRARLPR
                     LLRDGFLRSHEELRRSPV"
     gene            complement(1281..>2032)
                     /locus_tag="VG709_02110"
     CDS             complement(1281..>2032)
                     /locus_tag="VG709_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWC31905.1"
                     /translation="AIGSDARPGVCMPVDRCLADSVHLLAINTEERSATIVGFPRDSY
                     VEVPGHGSERINEALREGGPELLVDTVERVTGVRVDAYLLTSFEGFIGMIDALGGITV
                     EIPYPMHDASARTNFEPGPRRLDGLRALRFARDRKSGPNGDFSRSANHGTLMTAALRA
                     LQRDVQQDPADLFEWIVTGIRNLQTDLSFTEIFDLMVTALSIDKDRVANVVVPGGLGF
                     VGSASIVTLGNDAEVLFRDLRDDGLLKASRR"
BASE COUNT          280 a          690 c          693 g          369 t
ORIGIN      
        1 atccggccct gccccaccct gtcgaggcgg tgatcttcgg gctcgcgatc gtcggagccg
       61 cctttctcct ctcgtgggca gccgaggcgg cccaggtgga catctcggca gcgctcgcca
      121 tcgccgtgct ggcgctcatc gcggtgctac cggagtacgc cgtcgacttc gtgtttgcct
      181 tcaagggggg caagtcggtc caggcgttcg gcgagtcgtg tccggcgctg ggaggaggag
      241 ccgagtcgcc gtgctccctc gcgctcgcga acatgacggg ggcgaatcgc ctcctgatcg
      301 gggtgggctg gtcgatggtc gcgttcatcg cgtggtaccg ctaccgccgc ggcgtgcgtc
      361 cccggccgtc cgatgagcgg aacacgaccg cgagcggaat catcctcgac aggcaacact
      421 cgatcgagct cgggtacctt gcggttgcca ccctctactc gctcaccctt ccgctgaggg
      481 aatcgatcac cctggtcgac gcggcgatcc tcgtctcgat cttcgccgcg tacacctggc
      541 gcgtcgcgcg agcgcctgcc gaagacccgc acctcgtcgg gcctgcacgg tacgtcggcg
      601 gccggacgaa ggcgacgagg cgagcgatgg ttgcggcgat gtttctctac gccgccgcga
      661 tgatcctgtt gacggccgag ctcttcgccg aagcgcttgt cgcgaccggc atcgagctcg
      721 ggatcagcga attcttcctc gtgcaatggc tcgcgccgct cgcgtcggag acgccggagc
      781 tgttggtcgc ctcactgttc gcctggcggc tggcgacgaa cgcaggcctc ggcacgctcg
      841 tctcttcgaa ggtgaaccag tggaccctgc tggtcggaac gttgccggtg gtgttcacga
      901 tcgctgtcgg agagctccac ggactgccga tcgacagccg tcagcggcag gagatcttcc
      961 tgaccgcggc gcagtcgttc ttcgccgtcg cgatcctctc gaacctctcg atgtccgtgc
     1021 gggaggcgtg gatgttgttc gggctcttct gggcgcagtt catcctcggc gccttcatcc
     1081 cggaggagtt ccacgggacg gagctcgtga tcgtatcggt gatctacctc cttctcggca
     1141 tgatcgtgct cgtccgggat cgcgcgcggc tcccgcggct gttgcgcgac ggattcctac
     1201 gctcgcacga ggagcttcgc cggtctcccg tgtgacggag ggcgttgacg gttccccgcg
     1261 ccgtggcgtc gcgcggacag ttagcgccga gacgctttca gcagcccgtc gtcccggaga
     1321 tcgcggaaaa gcacctccgc gtcgttgccg agcgtgacga tgctcgcgct cccgacgaag
     1381 ccgagcccgc caggcacgac gacgttcgcg acgcggtcct tgtcgatcga cagcgccgtc
     1441 accatgaggt cgaagatctc ggtgaacgac agatcagtct ggaggttgcg gatccccgtg
     1501 acgatccact cgaagaggtc ggcagggtcc tgctggacgt cgcgctggag cgcccgcagc
     1561 gccgccgtca tgagcgttcc gtggttcgcc gaccggctga agtccccgtt cggtccgctc
     1621 ttccggtcgc gcgcgaaccg cagggcccgg agcccgtcga ggcgccgagg ccccggctcg
     1681 aagttcgtcc tcgcggacgc gtcgtgcatc gggtacggga tctcgacggt gatcccgccg
     1741 agcgcgtcga tcatcccgat gaagccctcg aacgacgtga ggaggtaggc gtcgaccctg
     1801 acgccggtca cccgctcgac ggtgtcgacg aggagctccg gtcccccctc tcgcagcgcc
     1861 tcgttgatgc gctcggagcc gtggccgggg acctcgacgt aggagtcacg cgggaacccc
     1921 acgatcgtcg cgcttcgctc ctcggtgttg atcgcgagga ggtggaccga gtcggcgagg
     1981 caacggtcga ccggcatgca gacgcccggc cgggcgtcgc tgccgatcgc ga
//
LOCUS       DASZOB010000133         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_73406, whole genome shotgun sequence.
ACCESSION   DASZOB010000133 DASZOB010000000
VERSION     DASZOB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73406"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..867)
                     /locus_tag="VG709_02115"
     CDS             complement(232..867)
                     /locus_tag="VG709_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC31906.1"
                     /translation="MKARVVLVDDHALFLAGVRAELAGAVEVVGEAATVADAVPLIKE
                     LDPDVVLLDVHLPDGGGDAVINAVAPERPGVRFLALSVSDAAEDVIGVIRAGARGYVT
                     KTISGEELTSAIERVRSGDAVFSPRLAGFVLDAFRAGERVAGDAELNELTPREREVLQ
                     LIARGYMYKEIAARLHLSVKTVESHVSNVLRKLQLSTRHELTRWATQHRLI"
     gene            complement(864..1937)
                     /locus_tag="VG709_02120"
     CDS             complement(864..1937)
                     /locus_tag="VG709_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWC31907.1"
                     /translation="MALPRFALDTDRRAIAGVCAGIARALGADVTLVRLVFAVLALAG
                     GAGILVYLALWAYARARRVWLTVLLAFVSGAIVLSAIGFGAGGVFGLALVVAGLWLAL
                     RRGGSLRPGAPVSYLGLGLAASGVAIAVPGGTPTLLAPGAVAAALIILVGPWLWRLVR
                     DRDAERAARVRSEERSDMAARVHDSVLQTLALIQRHAGEPQRVSALARRQERELRGWL
                     YGDLPLGNGAASLGAALAATAADVEEAHGVRVELASGGDCPLDDSVEALVLAAREAMT
                     NAAKFAGTERIDVYAEVTDDAVAVFVHDRGAGFDRAAVPPDRRGLVDSIEGRLERAGG
                     QATIVSAPGEGTEVELRVPRRSS"
     gene            complement(1943..2800)
                     /locus_tag="VG709_02125"
     CDS             complement(1943..2800)
                     /locus_tag="VG709_02125"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWC31908.1"
                     /translation="MSERMADTASTDELRRSRSNRMLAGVAGGLGAYFELHPAVFRVS
                     FVVLTLMGGAGILIYLAAALVMPDEGKEDSAATAALRRRRPWVLVVLGLAAVVPLALL
                     SEVPVWPQGDAWPFLLIAGALALWLTWFAVAGVRRGWLRAVGFVVASVAVLLVVLVAA
                     FLATFDVHLGDGVDERTVAPASVSALDRRYELGLGELVFDLRSVEFPLGETRLDVRVD
                     AGRIHVLLPDDVALRAVTEARLGEIDLLGRRVEGWEVDEQVDETGLRVLVLDAEVGIG
                     EIDIERGLR"
BASE COUNT          457 a         1120 c         1046 g          344 t
ORIGIN      
        1 ccgcacaacc atgcgaaatc ggatcgcgag aaagccgaaa ggcgcatgaa tagagcggtt
       61 ccgcgtccat cgtcgtcgac cgtcgccacc gtcgaagtgg cctgtcacgc cggaggtcgc
      121 gggttcgagt cccgtcgctc ccgtcctcaa tgtccctggt aacgggaaga ctcgtcatag
      181 cgggcgagct tccccaacgc accgacagac cctcgccctc gtgcctcctc tctagatcag
      241 gcggtgctgg gtcgcccagc gcgtgagttc gtgcctggtg gacagctgga gcttgcgcag
      301 gacgttcgag acgtggctct cgaccgtctt caccgacagg tgcaggcgcg cggcgatctc
      361 cttgtacatg tacccgcgcg cgatgagctg gaggacctcg cgctcgcgcg gggtcagctc
      421 gttcagctcc gcgtcgcccg cgacgcgctc gccggcgcgg aacgcgtcga gcacgaagcc
      481 ggcgagccgc ggcgagaaga ccgcgtcccc ggacctgact cgctcgatcg cggacgtcag
      541 ctcctcgccg gagatcgtct tcgtcacgta tccgcgcgcg cccgcccgga tcacgccgat
      601 cacgtcctcg gctgcgtccg agacggacag ggccaggaag cgcacgcccg gccgttcggg
      661 cgcgacggcg ttgatgaccg cgtcgccgcc gccgtccgga aggtgcacgt cgaggaggac
      721 gacgtccggg tcgagctcct tgatcagcgg cacggcgtcg gccacggtcg cggcctcgcc
      781 gacgacctcc accgccccgg cgagctccgc ccggacgccg gcgaggaaga gcgcgtggtc
      841 gtccaccagg acgacgcggg ccttcacgac gacctccggg gcacgcgcag ctcgacctcg
      901 gttccctcgc ccggcgcgct gacgatcgtc gcctgccctc ccgcccgctc gagccggccc
      961 tcgatcgagt cgacgagccc gcgccggtcc ggcgggacgg ctgcgcggtc gaagcccgcc
     1021 ccgcggtcgt ggacgaagac cgcgacggcg tcgtccgtga cttcggcgta gacgtcgatc
     1081 ctctccgtgc cggcgaactt cgccgcgttc gtcatcgcct cgcgagctgc tagcacgagc
     1141 gcctcgaccg agtcgtcgag cggacagtca ccaccgcttg cgagctcgac acggacgccg
     1201 tgggcctcct cgacgtccgc cgcggtggct gcgagcgccg ccccgagcga ggcggcgccg
     1261 ttcccgagcg ggaggtcgcc gtagagccag ccgcgcagct cccgctcctg ccggcgcgcc
     1321 agggcggaca cgcgctgcgg ctcgcccgcg tgccgctgga tcagcgccag cgtctggagg
     1381 acggagtcgt gcacccgcgc ggccatgtcc gatcgctcct cgctccgcac ccgcgccgcc
     1441 cgctcggcgt cgcggtcgcg gacgagccgc cagagccagg ggccgacgag gatgatgagc
     1501 gcggccgcca ccgctcccgg cgcgagcagt gtcggcgtgc cgccgggaac cgcgatcgcc
     1561 actccgctgg ccgcgaggcc gaggcccaag taggacacgg gggcgcccgg gcggaggctg
     1621 ccgccgcgac gcagcgcgag ccagaggccg gcgacgacga gcgcgaggcc gaagaccccg
     1681 cccgcgccga agccgatcgc gctcagcacg atcgcaccgg agacgaaggc aaggagcacc
     1741 gtcagccaca cccgccgcgc ccgcgcgtac gcccagagcg cgagatagac gaggatcccg
     1801 gccccgcccg cgagcgcgag cacggcgaag acgagccgga cgagggtcac gtcagctccg
     1861 agcgcgcgcg cgatccccgc gcagactccc gcgatcgcgc gccggtcggt gtccagcgcg
     1921 aaccggggca gcgccatcgc gctcatcgga ggccccgctc gatgtcgatc tcaccgatgc
     1981 cgacctccgc gtcgaggacg agcacgcgga gaccggtctc gtcgacctgc tcgtccacct
     2041 cccagccctc gacccgccga cccagcaggt cgatctcgcc gagcctcgcc tcggtgaccg
     2101 cccgcagggc gacgtcgtcc gggaggagca cgtggatcct gcccgcgtcg acgcgcacgt
     2161 cgagccgggt ctcgccgagg gggaactcga cggagcgcag gtcgaagacg agctcgccga
     2221 gccctagctc gtagcggcgg tcgagggcgc tcacgctcgc cggcgcgacc gtgcgctcgt
     2281 cgacgccgtc gccgaggtgg acgtcgaagg tggcgaggaa ggcggccacg agcaccacga
     2341 gcaagacggc gaccgacgcg acaacgaatc cgaccgcgcg cagccaaccc cggcggacgc
     2401 ccgcgaccgc gaaccaggtg agccagagag cgagggcgcc cgcgatcagc aggaacggcc
     2461 aggcgtcccc ctgaggccag acgggtacct cggagagcag ggcgagcggg acgaccgcgg
     2521 ccaagccgag cacgaccagg acccacgggc ggcggcggcg gagcgccgcc gtggcggccg
     2581 agtcctcctt cccctcgtcc ggcatcacga gcgccgccgc caggtagatc aggatccccg
     2641 cgccgcccat gagcgtgagg acgacgaagg agacgcggaa gactgcgggg tggagttcga
     2701 agtaggcgcc cagccctcct gccacgcccg ccagcatgcg gttcgagcgg ctgcggcgca
     2761 gctcgtcggt cgaggcggtg tcagccatgc gctcgctcat gcggccagga tctcaggctc
     2821 ggcctcgaaa cgcgcatccg ccggccgctc gtgctcgaac cgcggcagcc actcgagcca
     2881 ggacggcagg taccagttcc agtcgccgag cagcttcatc gtggccggca gcagcacggc
     2941 ccgcacgatc gtggcgtcga tgaggat
//
LOCUS       DASZOB010000134         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_164287, whole genome shotgun sequence.
ACCESSION   DASZOB010000134 DASZOB010000000
VERSION     DASZOB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164287"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1640
                     /locus_tag="VG709_02130"
     CDS             231..1640
                     /locus_tag="VG709_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWC31909.1"
                     /translation="MPPRPLEPSSEEMRAMLAAAGEYLERFVRELPDAPASDLAGAAE
                     MARDLTEPIPEEGRPFEEVLAVVERSAEKGFNTAGPGYLAYIPGGGLFAAALSELVAG
                     TINRFVNLWSPAPALAQMEWQAVRWLCDLFDYPPEARGILTSGGSLSEFSAIVTARKA
                     LLPRDFLGGTLYVTDQTHASVAKSAYLAGFPPESVRIVPRTPELRMDPDTLADAVKRD
                     RDDGRLPFCVVASAGTTNTGAIDPLDDIASLAADQGLWVHADAAYGGPFQITERGRRR
                     FRGIERSDSITLDPHKAMFLPYGTGALLVRDGMKLRDAHHVGADYLQDLAAEEEIPNF
                     TEYSPELSRNFRGLSVWLPLKLYGLRAWRDALDEKLDLTRHLYEELRATPGFEVPWEP
                     EVTVVAFRYVGTRGGDVDDLNVRLLDRINASKRVFLSSTRIDGAFWIRACIVSHRTHR
                     DRIEEAVEIIRSAAREIGA"
     gene            complement(1637..>2309)
                     /locus_tag="VG709_02135"
     CDS             complement(1637..>2309)
                     /locus_tag="VG709_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC31910.1"
                     /translation="HVHCFVLRLPERTILFDTGIGPPTAPAFAWTGASGRLWDELEAA
                     GVAREDVELVVISHTHDDHLGWTVDDDGAPAFPGARYLVHRADVEALRGSDDAEDREI
                     WRKTVAPLEAAGVLDANDAPTELDRGLTLIHAPGHTPGHQVLLVDRGERAVLSADVTN
                     HPALVEQPKWHQQTDTDPELASETRAELLERFERERRLYVASHFAEPRGTIVSGRDAR
                     RFRPL"
BASE COUNT          325 a          839 c          820 g          325 t
ORIGIN      
        1 gccgcgagcg gcggcagcgc gtagagacag cggaagaccg cgaccgtcga cggcgactcg
       61 ccggagagcc ggacgaagat cccggtgaag gcgatcgcga gcgccccgat ggccgccgtg
      121 cccatcggac gatcgccgac acgtgccatg tgggagcggc gctcggacgt catcgcgctc
      181 ttcaacgtac ccggacgtga gtcgattccg gcgctgctac cctcgccgcc atgccgcccc
      241 gaccgctcga gccctccagc gaggagatgc gggcgatgct cgcggcggcc ggcgagtacc
      301 tcgagcggtt cgtccgtgag ctaccggacg cgccggcgtc ggaccttgcg ggcgccgccg
      361 agatggcgcg cgacctcacg gagccgatcc cggaagaagg acgcccgttc gaggaggtgc
      421 tcgcggtcgt cgagcgctcc gcggagaagg ggttcaacac cgccggcccc ggctacctcg
      481 cctacatccc cggcggcggc ctgttcgcgg ccgcgctctc cgagctggtc gccggcacga
      541 tcaaccgctt cgtcaacctg tggagccccg ccccggcgct cgcgcagatg gagtggcagg
      601 cggtgcggtg gctctgcgat ctcttcgact acccgcccga ggcgcgaggg atcctcacgt
      661 cgggcggctc gctgtcggag ttctcggcga tcgtcaccgc gcgcaaggcg ctgctgccgc
      721 gggacttcct cggcgggacg ctctacgtga ccgaccagac acatgcctcg gtcgcgaagt
      781 ccgcgtacct cgcgggcttc ccgcccgaga gcgtccgaat cgtcccgcgg acgcccgagc
      841 tccgcatgga cccggacacg ctcgccgacg ccgtgaagcg cgaccgcgac gacggccgcc
      901 tcccgttctg cgtcgtggcc agcgccggca cgacgaacac cggcgccatc gacccgctcg
      961 acgatatcgc gtcgctcgcg gcggaccagg ggctgtgggt gcacgccgac gcggcgtacg
     1021 gggggccgtt ccagatcacc gagcgcgggc ggcggcggtt ccgcgggatc gagcgatccg
     1081 actcgatcac gcttgacccg cacaaggcga tgttcctccc ctatgggacc ggggcgctcc
     1141 tcgtccgcga cggcatgaag ctgcgcgacg cgcaccacgt cggggcggac tatctgcagg
     1201 acctcgccgc ggaggaggag atcccgaact tcaccgagta ctcgccggag ctgtcgcgga
     1261 acttccgcgg gctgtcggtc tggcttccgc tgaagctgta cgggctccgg gcgtggcgcg
     1321 atgcgctcga cgagaagctg gatctcaccc ggcatctgta cgaggagctc cgggcgacgc
     1381 ccgggttcga ggtcccgtgg gaacccgagg tgacggtcgt cgcgttccgg tacgtcggca
     1441 cacgcggcgg ggacgtcgac gacctcaacg tccggctgct cgaccgcatc aacgcgtcca
     1501 agcgcgtctt cctctcgtcg acgcggatcg acggcgcctt ctggatccgc gcgtgcatcg
     1561 tgagccaccg cacgcaccgc gaccgcatcg aggaagccgt cgagatcatc cgctcggccg
     1621 cgcgcgagat cggcgcctag agcggccgga accgccgggc gtcccgaccc gacacgatcg
     1681 tcccacgcgg ctcggcgaag tggctggcca cgtacagccg ccgctcgcgc tcgaaccgct
     1741 ccaggagctc ggcgcgagtc tcggacgcga gctcgggatc ggtatccgtc tgctggtgcc
     1801 acttcggctg ctcgacgagc gcgggatggt tcgtgacgtc cgccgacagg acagcgcgct
     1861 cgccgcggtc gacgaggagc acctgatggc cgggcgtgtg cccgggcgcg tggatcagcg
     1921 tgagcccgcg gtcgagctcc gtcggcgcgt cgttcgcgtc gagcacgcca gccgcctcga
     1981 gcggcgcgac cgtcttccgc cagatctcgc ggtcctctgc gtcatcgctc ccgcggagcg
     2041 cctcgacgtc ggcgcgatgg acgaggtagc gcgctccggg gaacgccggc gcgccgtcgt
     2101 cgtcgacggt ccacccgagg tggtcgtcgt gcgtgtgcga gatcacgacg agctccacgt
     2161 cctctctcgc gacgccggcc gcctcgagct cgtcccacag ccgtcccgat gcgcccgtcc
     2221 acgcgaacgc cggtgccgtc ggcggcccga tgccggtgtc gaagaggatc gtccgttcgg
     2281 ggagccggag aacgaagcag tggacgtgg
//
LOCUS       DASZOB010000135         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_144007, whole genome shotgun sequence.
ACCESSION   DASZOB010000135 DASZOB010000000
VERSION     DASZOB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144007"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VG709_02140"
     CDS             complement(<1..278)
                     /locus_tag="VG709_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWC31911.1"
                     /translation="MDVDTPAISSLEGSGLAYSVVRTEGPRSVEESAALQGIEVRQLL
                     RTIVVRRRAGDYVFLLVPGGRQIDWPKLRAHLGVSRLSLPDAAEAKAAT"
     gene            377..450
                     /locus_tag="VG709_02145"
     tRNA            377..450
                     /locus_tag="VG709_02145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:409..411,aa:Gln,seq:ctg)
     gene            452..524
                     /locus_tag="VG709_02150"
     tRNA            452..524
                     /locus_tag="VG709_02150"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:486..488,aa:Glu,seq:ctc)
     gene            complement(543..1136)
                     /locus_tag="VG709_02155"
     CDS             complement(543..1136)
                     /locus_tag="VG709_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31912.1"
                     /translation="MTERSWSVLLVGGASGVGKSAVSYRLAAELGVNLTAVDDVQNIL
                     ERMTDPDRYPVVHLWRLHPERLLALEEAGMLAHTLEYAAVVAEALEPVIASHLQSGVP
                     LMLEGDFILPSLAIREAYDGVPADGRVRALFLYEEEEAQIARNFAAREGEEQPFRARA
                     CWRYSEWLRDECRRLGVTAIPARPWETVLDRALGAVS"
     gene            complement(1133..>1731)
                     /locus_tag="VG709_02160"
     CDS             complement(1133..>1731)
                     /locus_tag="VG709_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31913.1"
                     /translation="DRRPTDWERNVARLETAEGITADMAIRRDALEELGGFDERFRRA
                     YREDTDLMIRMRSKAHRLANGRRTVLHPVRPASRWISVSMQRGNADDALMYALHGRSW
                     ADGGRWSLGTRRAHIATTVAGLVAVAAVGARARRLAAVAGGAWAVATGALAWKRIRPG
                     PRDPDEITTMIVTSAAIPPAATYWWVVGLVRRRGSSSR"
BASE COUNT          253 a          640 c          592 g          246 t
ORIGIN      
        1 gtcgcggcct tcgcctcggc cgcgtccggg agggagaggc ggctgacgcc caggtgcgcg
       61 cggagcttcg gccagtcgat ctgccgcccg ccggggacca ggaggaagac gtagtcgccg
      121 gcgcgacgac ggacgacgat cgtgcgaagg agctgccgga cctcgatccc ctgcagcgcg
      181 gcgctctcct cgacgctcct cggtccctcg gtccgcacga cggagtacgc gagcccggag
      241 ccctctaggc tgctgatggc gggggtgtcg acgtccacga cgcggaggat acgaagcggg
      301 cgtgcccaac gtgctcgacg tcgtcatgcc gagactcgag ccgacgccgg tgatacgctt
      361 ttccggtcag acgtggtggg tcgtggggta acggtagccc ggctgactct ggatcagcta
      421 gtcctggttc gaatccaggc gacccagcca aggtcccttc gtctagtggc ccaggacacc
      481 accctctcaa ggtggagaca cgggttcgaa ccccgtaggg accaccgcgc acgtcgagat
      541 tcttacgaca ccgcgccgag cgcgcgatcg aggaccgtct cccacggacg agccgggatc
      601 gccgtgactc ccagccgccg gcattcgtcg cggagccact cgctgtaccg ccagcaggcg
      661 cgggcccgga acggctgctc ctccccctcg cgagcggcga agttgcgagc gatctgtgcc
      721 tcctcctcct cgtagaggaa cagcgcgcgg accctcccgt cggccgggac gccgtcgtac
      781 gcctcccgta tcgcaagcga ggggaggatg aagtccccct cgagcatcag cgggacgccg
      841 gactggaggt ggctcgcgat caccggctcg agcgcctcgg cgaccacggc cgcgtactcg
      901 agcgtgtgcg cgagcatgcc cgcctcctcg agcgcgagca gccgctcggg gtggagccgc
      961 cagaggtgga cgaccgggta gcgatccgga tcggtcatcc gctcgaggat attctggacg
     1021 tcgtccacgg ccgtgaggtt cacccccagc tcggccgcga gccggtagct caccgcggac
     1081 tttccgacgc cgctcgctcc cccgacgagg agcacgctcc acgagcgctc cgtcaccgcg
     1141 acgacgaccc ccgtcgtcgc acgagcccga ccacccacca gtacgtcgcc gccggcggga
     1201 tcgccgcgct cgtcacgatc atcgtggtga tctcatcggg atcgcgagga cccgggcgga
     1261 tcctcttcca cgcgagcgct cccgtcgcga cggcccacgc gccgcccgcg actgcggcga
     1321 gccgccgagc gcgcgcgccc acggcagcga cggcgacgag gcctgcgacg gtcgtcgcga
     1381 tgtgcgcgcg tctcgttccg agcgaccacc tgccgccgtc ggcccacgaa cggccgtgga
     1441 gcgcgtacat cagcgcgtcg tccgcattcc cgcgctgcat cgacacgctg atccatcgcg
     1501 acgccggccg gaccggatgg aggaccgtgc ggcgcccgtt cgcgagccga tgtgccttcg
     1561 accgcatgcg gatcatgagg tccgtgtcct cccggtacgc gcggcggaac cgctcgtcga
     1621 agccgccgag ctcttcgagc gcatcccggc ggatcgccat gtcggcggtg atcccttccg
     1681 cggtctcgag gcgagcgacg ttgcgctccc agtccgtcgg ccgccggtcg g
//
LOCUS       DASZOB010000136         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_53311, whole genome shotgun sequence.
ACCESSION   DASZOB010000136 DASZOB010000000
VERSION     DASZOB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53311"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /gene="scpB"
                     /locus_tag="VG709_02165"
     CDS             <1..453
                     /gene="scpB"
                     /locus_tag="VG709_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWC31914.1"
                     /translation="VERALEELAERRAGSGIVVRSIAGGWRLYTHPGAAPAVERFVLS
                     SRHVRLTRAALETLAVVAYKQPVTRHQVSAIRGVDSDGVLRALVDRGLLEEVGRDDGP
                     GRPVLFGTTPEFLERLGLSSVADLPPLAPLLGEREQEEGDAGAEERAG"
     gene            425..1159
                     /locus_tag="VG709_02170"
     CDS             425..1159
                     /locus_tag="VG709_02170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWC31915.1"
                     /translation="MPAPRSGPAEPERLQKVLARAGIGSRRAAEDLIREGRVTVGGRV
                     ATLGDRVDPVKDRVEVDGVRVPLDPGLRYYAFHKPRGVVTSARDPQHREDISGYLPEG
                     PRVFPVGRLDRDSEGLLLLTNDGELANRVMHPRYGVEKEYLAEVDGTPSERALSRLAR
                     GVQLDDGVARALSASRLARSGGRSAVRVIMREGRKREVRRMLAAVGLPVVRLVRVRVG
                     PVRLGRLQAGEVRVLEPEEIRELLRG"
     gene            1160..1828
                     /gene="cmk"
                     /locus_tag="VG709_02175"
     CDS             1160..1828
                     /gene="cmk"
                     /locus_tag="VG709_02175"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWC31916.1"
                     /translation="MDTRGRKPVVAIDGPAGAGKSTLARRIAEELRLSYVNTGLMYRA
                     LARVALREGIDVADGEGLGRRARDLSFSIGERGSQPSLTIDGSIPGPELSAADVEAVV
                     STVSAHPEVRAVLRAEQRRLAERGAVVEGRDIGTVVFPDADVKILLHADPAERAARRQ
                     AERGTRDPHLVDALARRDALDARVNPLIPAEDATRLETTGRDADEVFAEAMTIVRRAL
                     EAMA"
     gene            1825..>2225
                     /locus_tag="VG709_02180"
     CDS             1825..>2225
                     /locus_tag="VG709_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWC31917.1"
                     /translation="MSAVASGRGTSAPRVAIVGRQNVGKSTLLNRLLGTREAIADPRP
                     GVTRDRLEVPVTWRGRTFVAIDTGGYVQRPRGIDALVTDQADRAMAEADLILLVVDAQ
                     VGPQEEDALLARRLRRAAVPVVLVANKIDSDT"
BASE COUNT          298 a          778 c          847 g          302 t
ORIGIN      
        1 gtcgagcgcg cgctcgagga gctggcggag cgacgcgcgg gctccgggat cgtggtccgg
       61 tcgatcgctg gcggctggcg tctctacacg catccggggg ccgccccggc cgtcgaacgg
      121 ttcgtgctct cgtcgcgcca cgtgcggctc acgcgtgccg cgctcgagac gctcgcggtc
      181 gtcgcgtaca agcagccggt cacgcgccac caggtctcgg cgatccgcgg cgtcgactcc
      241 gacggcgtcc tgcgcgcctt ggtcgaccgc ggcctgctcg aggaagtcgg acgcgacgac
      301 gggccggggc gtcccgtcct gttcggcacg acgcccgagt tcctcgagcg tctaggactc
      361 tcgtcggtcg cggacctccc gccgctcgct ccgttgctgg gcgaaaggga gcaggaggag
      421 ggagatgccg gcgccgagga gcgggccggc tgagcccgag cggctccaga aggtcctcgc
      481 gcgcgccggt atcgggtcga gacgcgcggc cgaggacctg atccgcgagg ggcgcgtgac
      541 ggtcggcggg cgcgtagcga cactcggcga tcgcgtcgat ccggtgaagg accgggtcga
      601 ggtcgatggc gtccgcgtgc cgctcgatcc ggggctccgc tactacgcgt tccacaagcc
      661 gcgcggcgtc gtgacgagcg cgcgtgaccc gcagcatcgt gaggacatct ccgggtacct
      721 gccggaaggt ccgcgcgtgt tcccggtcgg ccgcctcgac cgcgacagcg aggggctcct
      781 gctgctcacc aacgacggcg agctggcgaa ccgcgtgatg cacccgcgct acggtgtcga
      841 gaaggagtac ctcgccgagg tcgacgggac acctagcgag cgggcgctct ctcgcctcgc
      901 ccgcggcgtc cagctcgacg acggagtggc gcgcgccctc tcggcgagcc gcctggcgcg
      961 ttcgggcggg cggtcagcgg tccgagtcat catgcgggag gggcgcaagc gcgaggtccg
     1021 ccggatgctg gcggcggtcg ggcttccggt cgtccggctc gtccgcgtcc gggtcgggcc
     1081 cgtgaggctc gggcgcctgc aggccggaga ggtccgtgtg ctcgagccgg aggagatccg
     1141 cgagctgctg cgaggctgaa tggacacccg aggacgcaag ccggtcgtcg ccatcgacgg
     1201 tcccgccggg gcggggaaga gcacgctcgc ccgccggatc gccgaagaac tccgcttgtc
     1261 gtatgtcaac accgggctca tgtaccgcgc gctggcgcgc gtcgcgctac gagaggggat
     1321 cgacgtcgcc gacggcgagg gcctcggacg gcgcgcgcga gacctctcgt tctcgatcgg
     1381 cgagcgcgga tcgcagccca gcctcacgat cgatggaagc atcccggggc ccgagctctc
     1441 agccgccgac gtcgaggccg tcgtctcgac ggtgtcggcg catcccgagg ttcgcgcggt
     1501 gctccgcgcc gagcaacgga ggctcgccga acgcggcgcg gtcgtcgagg gacgggacat
     1561 cggcaccgtc gtctttcccg acgccgacgt gaagatcctg ctccacgccg atccggcgga
     1621 acgcgccgct cggcggcagg ctgagcgcgg aacgcgcgat ccgcatctcg tggacgcgct
     1681 cgctcggcgc gacgccctcg acgcgcgcgt caacccgctc atcccagcgg aggacgcgac
     1741 gcggctcgag acgacagggc gcgacgccga cgaggtgttc gcggaggcga tgacgatcgt
     1801 ccgtcgcgcg ctggaggcca tggcgtgagc gccgtcgcga gcggcagggg gacgtccgcg
     1861 ccgcgggtcg cgatcgtcgg gcgccagaac gtcggcaagt cgacgctcct caaccggttg
     1921 ctcgggacgc gcgaggcgat cgccgatccg cgtccggggg tgacccgtga ccggctcgag
     1981 gtcccggtga cgtggcgcgg tcgcacgttc gtcgcgatcg acaccggcgg gtacgtacag
     2041 cgcccacgcg gcatcgatgc gctcgtcacc gaccaggcgg accgagcgat ggccgaggcg
     2101 gatctcatct tgctcgtcgt cgacgcgcag gtcgggcccc aggaggaaga cgcgttgctc
     2161 gcgcgccgcc tgcgcagggc ggcggttccg gtcgtcctcg tcgccaacaa gatcgactct
     2221 gacac
//
LOCUS       DASZOB010000137         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173107, whole genome shotgun sequence.
ACCESSION   DASZOB010000137 DASZOB010000000
VERSION     DASZOB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173107"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VG709_02185"
     CDS             complement(<1..619)
                     /locus_tag="VG709_02185"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWC31918.1"
                     /translation="MSESKAQPTALRRSDDRWVRGEYTDDELTAALLANGMAGEVSHD
                     RANVWGKVQRLVTGDPDVTFGLSGMTRYEAREVIGLVARAIDFAVEPNFLDGPTVIEP
                     ATILQALHEAGDRLALAADRGEEVLIATGHPVGMVMLYIEVARELSRRGAKLLRPLEG
                     FRWRELGRSRQIRYLHGVGVLTDRASSLHTHSPGPMERMLGEVEPD"
     gene            652..1428
                     /locus_tag="VG709_02190"
     CDS             652..1428
                     /locus_tag="VG709_02190"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC31919.1"
                     /translation="MPHFSLEGKVAIVTGGSKGIGRAIALEFAAAGADVALAARGEEE
                     LNLVAKEIQERDRRAIVVPTDVSDPAQIQALIDRTVGELGKLDVLVNNAGSAPFFSTI
                     DTIRESGFEKYFRVNFLSAFYATRYAAPHLLKNEGSSVVNVASVAGYIASPGLTYYST
                     AKAAMINLTKTVAREWANHGVRVNAVAPGWIESEMNAEARKSDAFYEGVVSMVPMGRW
                     GQPEEVAAVVRFLASPAASFMTGSVVIVDGGQTLASLVGS"
     gene            1415..>2220
                     /locus_tag="VG709_02195"
     CDS             1415..>2220
                     /locus_tag="VG709_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC31920.1"
                     /translation="MSAADGALFELAGRVVVVTGASSGIGWASAWALASRGAIVVPAA
                     RRVERLERLAEHITDRGGEALPIACDVSDRGSIDAMRDRVLERYGRCDALVNNAGIPG
                     GGPFTELSDERIEQVVRTNYLGVIWGTRAFLPTMLEQRRGHVVNVASLAGRYAIPFSA
                     VYSSTKHAVVAFSESLNYETAPQGVLVTSVNPGLVSTELFPHRDTAEGGGLRARLVMP
                     PERIAATIVRVLERGIAPEISVPRWLASFQAFRVVTPPLFRAGVRRAVAAG"
BASE COUNT          307 a          797 c          785 g          331 t
ORIGIN      
        1 ggtcgggctc gacctcgccc agcatgcgct ccatcgggcc ggggctgtgg gtgtgcaggc
       61 tcgacgccct gtcggtcagg acgccgaccc cgtgcaggta gcggatctgc ctcgagcgcc
      121 cgagctcgcg ccagcggaac ccctcgagcg gccggagcag cttcgcgccg cgtcgcgaga
      181 gctcgcgcgc gacctcgatg tagagcatca ccatcccgac cgggtgcccc gtcgcgatca
      241 gcacctcctc accgcggtct gcggccagcg cgagccggtc acccgcttcg tgcagcgcct
      301 ggaggatcgt cgccggctcg atcacggtcg gcccgtcgag gaagttcggc tcgaccgcga
      361 agtcgatcgc ccgggcgacg agcccgatga cctcccgcgc ctcgtagcgc gtcatcccgg
      421 agagcccgaa cgtcacgtcc gggtctccgg tcacgagccg ctggaccttg ccccacacgt
      481 tggcgcggtc gtgggagacc tcgccggcca tgccgttcgc gagcagcgcc gcggtcagct
      541 cgtcgtcggt gtactcgccg cgcacccacc ggtcgtcgct tcggcgcagc gcggtcggct
      601 gtgctttgct ttcggacatc cacccgatgg taaccgcagg aagggagacc catgcctcac
      661 ttctccctcg aggggaaggt cgcgatcgtg accggcggca gcaaggggat cggccgcgcg
      721 atcgcgctcg agttcgcggc ggccggcgcc gacgtcgcgt tggccgcgcg cggtgaggag
      781 gagctcaacc tcgtcgccaa ggagatccag gagcgggacc ggcgcgcgat cgtcgtcccg
      841 accgacgtct cggacccggc gcagatccag gcgctgatcg accgcacggt cggcgagctc
      901 gggaagctcg acgtcctcgt gaacaacgcc ggctcggcgc cgttcttctc gacgatcgac
      961 acgatccgcg agtccgggtt cgagaagtac ttccgcgtga acttcctcag cgcgttctac
     1021 gcgacgcggt acgcggcgcc gcaccttctg aagaacgagg gctcgagcgt cgtgaacgtc
     1081 gcctcggtcg ccggctacat cgcctcgccc gggctcacct attactcgac ggcgaaggcc
     1141 gcgatgatca acctcacgaa gaccgtcgcg cgcgagtggg cgaaccacgg cgtgcgcgtg
     1201 aacgcggtcg cgcccggctg gatcgagagc gagatgaacg ccgaggcgcg caagagcgac
     1261 gcgttctacg agggcgtcgt gagcatggtc cccatgggcc gctggggaca gccggaggag
     1321 gtcgccgcgg tcgtgcggtt cctggcgtcg cccgccgcct cgttcatgac cgggtccgtc
     1381 gtcatcgtcg acggcggaca gacgctcgcc tcccttgtcg gcagctgacg gcgcgctctt
     1441 cgagctcgcc gggcgcgtcg tcgtcgtcac cggcgcgtcg agcgggatcg gatgggcctc
     1501 cgcgtgggcg ctcgcgagca ggggcgccat cgtcgtcccg gcggcacgtc gcgtcgagcg
     1561 actggagcgc ctcgcggagc acatcaccga ccgcggcggc gaggcactgc ccatcgcgtg
     1621 cgacgtcagt gaccggggat ccatagacgc gatgcgcgac cgcgtcctcg agcgctacgg
     1681 acggtgcgac gcgctcgtca acaacgccgg catccccggc ggcggtccgt tcacggagct
     1741 gtccgacgaa cggatcgagc aggtcgtccg gaccaactac ctcggtgtca tctgggggac
     1801 gcgggcgttc ctcccgacga tgctcgagca gcgacgcggg cacgtcgtga acgtcgcgtc
     1861 gctcgcgggg cgatacgcga tcccgttctc agccgtgtac agctcgacga agcatgccgt
     1921 cgtcgcgttc agcgagtcgc tgaactacga gacggcaccg cagggggtgc tcgtcacgag
     1981 cgtgaacccg ggtctggtct cgacggagct cttcccccac cgcgacaccg cggagggcgg
     2041 cggcctgcgc gcgcggctgg tcatgccccc cgagcgcatc gccgcgacga tcgtccgggt
     2101 gctggagcgc gggatcgcgc cggagatctc ggtcccccgc tggctcgcgt cgttccaggc
     2161 gttccgcgtc gtgacgcctc cgctgttccg cgccggggtc cggcgcgccg tcgccgccgg
//
LOCUS       DASZOB010000138         4155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_41944, whole genome shotgun sequence.
ACCESSION   DASZOB010000138 DASZOB010000000
VERSION     DASZOB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41944"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1171
                     /locus_tag="VG709_02200"
     CDS             200..1171
                     /locus_tag="VG709_02200"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWC31921.1"
                     /translation="MTTLGKVLLAAPRGYCAGVDRAVDVVERALRIYGAPVYVRKQIV
                     HNIHVVRDLERKGAVFVEDESEVPEGATVILSAHGVAPEVYRNSAERQLRVIDATCPL
                     VTKVHKEAIRFAGDAYTILLIGHEGHEEVIGTSGEAPDHIRLVGDPDEARTVEVPDRD
                     RVAYLSQTTLSVDETNDVIRVLRERFPNIQGPPRDDICYATQNRQEAVKELAARSDVV
                     LVIGSDNSSNSNRLVEVARSLGTPAYLIDDETEIEERWLDGAETVGVTSGASAPEWLV
                     ERVMGHLRSLGATDVDEVRVRDEFVEFGLPAGVRESDARRVAATSAT"
     gene            complement(1168..2364)
                     /locus_tag="VG709_02205"
     CDS             complement(1168..2364)
                     /locus_tag="VG709_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWC31922.1"
                     /translation="MGDERPRTVKDLLAEAKDTSEIMVDLAYAAVFFNDEDLAEEVDR
                     LEERMDGYLGDLRWISMLAARSPEDAEAMGEVLHVAGSIEKIGDAAADIARVVTAHLG
                     IPEALRKDLRHADEIVGRLRVREGSQIVGRTLRDLSLPSEMGMWLVAIRRGDEWEHDP
                     GPDTAITGEDVLLYQGPEGGENLLRELAGAPLARPEPSEAIALTELDRAVDILIEMKN
                     SAEVAVGLAYSALLFTEPALAAEVATLEARSDALHDELESWVLRASPEARNPDDLRGL
                     IRLAYASEVIFDAAREMTRVVEQGEELHPVVAAAIEASDEVAAEALVEHGSRAAGTTL
                     KQLSVETETGMLVLAVQRGRRWIYRPRPSFELAARDRVIAIGPEDGIPELESLVRAPA
                     ETQAPV"
     gene            complement(2410..3630)
                     /locus_tag="VG709_02210"
     CDS             complement(2410..3630)
                     /locus_tag="VG709_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWC31923.1"
                     /translation="MRGGLRRILAYWRAERRTLGQGFVALVVASIGSLAGGVVLGSIT
                     GTLERLPGLLVLVPAAVAMRGNIFGAMASRLGTTIHSGLFEISRRREGPLMQNLYAAT
                     VLTLTVSVVVAVFAKAVSLAFGDDSMSLLDFIVLSLVGGVLASLFVGALSVGLSLLAY
                     RRGWDLDTVAAPLLTAAGDMLTIPALFVATSLAEIRFVTPTLAVVSLIVAVWATIAGL
                     RTNLAVTRRILRESLPVLFFAGLVDILAGQFVEARLEQFAAFPALLMLIPPVLGNAGG
                     LGGMLSSRLASKLHLGVMPPQGLPASLAVIDFFTVFLIALAVFPLMGYAGALLAPVFG
                     LESPGIVNMIEVATLAGVMATLLATVVAYYSAVATYRLGWDPDNHGIPMITSSMDFVG
                     VVCFVIALVAVGIV"
     gene            complement(3716..4003)
                     /locus_tag="VG709_02215"
     CDS             complement(3716..4003)
                     /locus_tag="VG709_02215"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWC31924.1"
                     /translation="MAEPLSDEEIAERLRDLPAWRHERGELHKWFKFAGFPDAIAFLQ
                     RIVEPAERLNHHPDVENHYNRVRIGLHTWSAEAVTEKDFALAREIERAASA"
     gene            complement(4003..>4155)
                     /locus_tag="VG709_02220"
     CDS             complement(4003..>4155)
                     /locus_tag="VG709_02220"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291137.1"
                     /note="catalyzes the formation of succinate semialdehyde
                     and glutamate from 4-aminobutanoate and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWC31925.1"
                     /translation="IEECYRQGLIVLKAGTYDNVVRLLPPLTIDTPLLDEGLEILEKA
                     VAVAAG"
BASE COUNT          678 a         1430 c         1467 g          580 t
ORIGIN      
        1 cgctccacct cctcttaggg atctggccgt cgtggggaag gggggacggc attcgggcaa
       61 acaaacgccg ccggcgttac cctgctcggc ggctcgagaa accctatcag acggatgtcg
      121 tccggaacga ccacgccgcc cgttccggtc gccctgggcg tctcgcggga agccgacgac
      181 cgtccagtac gatgggtgtg tgacgacgct cggaaaggtg ctgctcgcgg cgccgcgcgg
      241 atactgcgcg ggcgtcgacc gcgccgtcga cgtcgtcgag cgcgcgctgc gcatctacgg
      301 cgcgccggtc tacgtgcgga agcagatcgt ccacaacatc cacgtcgtcc gggatctcga
      361 gcgcaagggc gccgtcttcg tcgaggacga gagcgaggtg ccggagggcg ccacggtgat
      421 cctgtccgcg cacggcgtcg cgccggaggt gtaccggaac tccgccgaac gccagctccg
      481 ggtgatcgac gcgacgtgcc cgctcgtcac gaaggtccac aaggaggcga tccggttcgc
      541 cggcgacgcc tacacgatcc ttctgatcgg ccacgagggc catgaggagg tcatcgggac
      601 cagcggagag gcacccgacc acatccgcct cgtcggcgac ccggacgagg cccggaccgt
      661 cgaggtgccc gaccgcgacc gcgtcgcgta tctctcgcag acgacgctgt ccgtcgacga
      721 gaccaacgac gtgatccgcg tcttgcgaga gcggttcccg aacatccagg gaccgccgcg
      781 cgacgacatc tgctacgcga cgcagaaccg ccaggaggcc gtgaaggagc tcgctgctcg
      841 atccgacgtc gtgctcgtga tcggctccga caactcctcg aactcgaacc gcctcgtcga
      901 ggtcgctcgc tccctgggga cgcccgccta cctcatcgac gacgagaccg agatcgagga
      961 gcggtggctc gacggcgcgg agacggtcgg cgtgacgtcc ggcgcgtcgg ccccggagtg
     1021 gctcgtcgag cgagtgatgg gacacctacg ctcgctcggc gcgaccgacg tcgacgaggt
     1081 ccgcgtccgt gacgagttcg tagagttcgg cctgcccgcc ggcgtcaggg agtccgacgc
     1141 ccgccgcgtc gcggcgacct cggcgaccta gaccggggcc tgggtctcgg ccggagcgcg
     1201 gaccagcgac tcgagctccg ggatgccgtc ctcggggccg atcgcgatga cgcggtcacg
     1261 tgcggcgagc tcgaagctcg ggcgtgggcg gtagatccac cgtcgccccc gctgcacggc
     1321 cagcacgagc atccccgtct cggtctccac ggagagctgc ttgagcgtcg tccctgccgc
     1381 gcgcgagccg tgctcgacga gcgcctcggc cgcgacctcg tcgctcgcct cgatcgcggc
     1441 cgcaacgacg gggtgcagct cctctccttg ctccacgacg cgagtcatct cgcgggcggc
     1501 gtcgaagatc acctcggacg cgtacgcgag gcggatcagt ccgcgcagat catcggggtt
     1561 ccgcgcttcc ggcgaggccc gcagcaccca tgactcgagc tcgtcgtgga gggcgtcgct
     1621 gcgggcctcg agcgtcgcga cctcggccgc gagcgccggc tccgtgaaga gcaacgccga
     1681 ataggcgagg cccaccgcga cctccgcgga gttcttcatc tcgatgagga tgtcgacggc
     1741 cctgtcgagc tcggtgagcg cgatcgcctc cgacggctcg ggcctcgcga gcggcgcgcc
     1801 ggcaagctcg cggaggaggt tctcgcctcc ctcgggcccc tggtagagaa ggacgtcctc
     1861 gcccgtgatc gcggtatccg ggccggggtc gtgctcccat tcgtcgccgc ggcggatcgc
     1921 gacgagccac atccccatct cgctggggag cgagaggtca cggagcgtcc gtccgacgat
     1981 ctgcgatccc tcgcgcacgc gcaggcggcc gacgatctcg tcggcgtgac ggaggtcctt
     2041 gcggagggcc tcggggatgc cgaggtgcgc cgtcacgacg cgggcgatgt ccgcggccgc
     2101 gtcgccgatc ttctcgatcg agccggcgac gtggaggacc tcacccatcg cctcggcgtc
     2161 ctctggactc cgcgcggcga gcatggagat ccaccggagg tcaccgaggt agccgtccat
     2221 ccgctcctcg aggcggtcga cctcctcggc gaggtcctcg tcgttgaaga acacggcggc
     2281 gtaggcgagg tcgaccatga tctcggaggt gtctttcgcc tccgcgagga gatccttgac
     2341 tgttcgggga cgttcgtcac ccatgagtgg tcgctccagg agtatagcca gcaaacgcgc
     2401 aacgcctcat cagacgatcc cgacggcaac gagcgcgatc acgaagcaga cgacgccgac
     2461 gaagtccatc gagctcgtga tcatcgggat gccgtggttg tccgggtccc acccgagccg
     2521 gtacgtcgcc acggccgagt agtaggccac gacggtcgcg agcagcgtcg ccatgacgcc
     2581 ggcgagcgtc gcgacctcga tcatgttgac gatgccgggg ctctcgagac cgaagaccgg
     2641 cgcgagcagg gcgcccgcgt accccatcag cgggaagacg gccagcgcga tcaggaagac
     2701 cgtgaagaag tcgatgacgg cgagcgacgc gggcagaccc tgcggcggca tgaccccgag
     2761 gtggagcttc gaggccaagc gcgacgagag catcccgccg agcccgccgg cgtttcccag
     2821 gaccggtggg atcagcatca agagggccgg gaaggccgcg aactgctcta gccgcgcctc
     2881 gacgaactgc ccggcgagga tgtcgacgag acccgcgaag aagaggaccg gcaggctctc
     2941 ccggaggatc cggcgcgtga cggcgagatt cgtccgaagc ccggcgattg tcgcccagac
     3001 ggcgacgatg agggagacga cggcgagcgt cggcgtcacg aagcggatct ccgcgaggga
     3061 cgtcgcgacg aagagcgccg ggatcgtcag catgtcgccg gcagccgtga ggaggggggc
     3121 cgcgaccgta tcgaggtccc atccgcgccg gtacgcgagc agcgaaaggc ctacggagag
     3181 agcaccgacg aacaggctcg cgagcacgcc gccgacgagc gagagcacga tgaagtccag
     3241 cagtgacatg ctgtcgtccc cgaacgcgag cgagacggcc ttcgcgaaca cggcgaccac
     3301 gaccgacacg gtgagcgtga ggaccgtcgc cgcgtagagg ttctgcatca gcggaccctc
     3361 gcgccgacgg ctgatctcga acaggccgct gtggatcgtc gtgccgaggc gcgacgccat
     3421 cgcgccgaag atgttgccgc gcatggcgac cgccgccggc acgaggacga ggaggcccgg
     3481 gagccgctcg agcgttccgg ttatcgagcc gaggactact ccgccggcga ggcttccgat
     3541 cgacgcgacg acgagggcga cgaacccctg tccgagggtt cgccgctcag cgcgccagta
     3601 ggccaggatc cttctgaggc cacctcgcat cgcgcacccg ccatcgctcg agtcccccgg
     3661 ggaagaaagc cggagggagt atctcccccg gcacgtgcgc gctcaacctg cggcgctacg
     3721 cagacgccgc tcgctcgatc tcgcgggcga gcgcgaagtc cttctcggtc acggcctcgg
     3781 cgctccaggt gtggagcccg atccgaacgc ggttgtagtg gttctcgacg tcggggtggt
     3841 ggttcagccg ctcggcgggc tcgacgatcc gctggaggaa cgctatggcg tccgggaacc
     3901 cggcgaactt gaaccacttg tggagctcgc cgcgctcgtg ccgccacgcg gggagatcgc
     3961 ggaggcgctc ggcgatctcc tcgtcggaga gcggctccgc catcagcctg ccgcgaccgc
     4021 gacggccttc tcgaggatct cgaggccctc gtcgaggagc ggcgtgtcga tcgtgagcgg
     4081 cggcaggagc cgcacgacgt tgtcgtacgt ccccgccttg aggacgatca ggccctgccg
     4141 gtagcactcc tcgat
//
LOCUS       DASZOB010000139         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132777, whole genome shotgun sequence.
ACCESSION   DASZOB010000139 DASZOB010000000
VERSION     DASZOB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132777"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      100..228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            297..1403
                     /gene="ribD"
                     /locus_tag="VG709_02225"
     CDS             297..1403
                     /gene="ribD"
                     /locus_tag="VG709_02225"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWC31926.1"
                     /translation="MSVDEAYMRRALELAARGRGVTSPNPLVGAVVVKDGRVVAEAFH
                     EGAGRRHAEAVALDAAGDEARDATLYVNLEPCVHHGRTPPCAPRVIESGVWRVVVAMP
                     DPNPLVDGRGFRALRDAGIEVTTGVLEHESRRLNEAFLTHVRTGRPFVTLKMAASLDG
                     KVAARDGSSRWITGGEAREDVHRMRAEVDAIMVGAGTAFRDDPSLTVRVAGYHGRGKL
                     RVLVDGRGIVPESHRLFAEGGAPTLVATTEGAPEDRRRAWADAGADVVVIDDDGRSLI
                     ELDALFRELGKRDVQHVLLEGGPTLAWEAVRRDLVDRVVLFFAPIFVGGDRAPSVLMG
                     DGTPTIGEARPVAIESVERVGDDIKVVARVHRDH"
     gene            1417..1977
                     /locus_tag="VG709_02230"
     CDS             1417..1977
                     /locus_tag="VG709_02230"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWC31927.1"
                     /translation="MTAARRTDGVAQIAVTGPKVAEDAEVGSSVAVSGVCLTVVERRG
                     DGMSFDVVDETLSRTTLGDLRAGDVVNLERPVTLATRLGGHLVQGHADAVGTVRSVEW
                     SNSGSVMAIEAAPDVLRYVVEKGSVAVDGVSLTVARAGGDAFDVALVPHTLAVTTLGE
                     RDASDRVNLEIDIVAKYIEGLIGRPG"
     gene            1974..3260
                     /locus_tag="VG709_02235"
     CDS             1974..3260
                     /locus_tag="VG709_02235"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWC31928.1"
                     /translation="MSEERTTVEARADETTVAASAFSPIEDAIEDVRRGRIVIVVDDA
                     DRENEGDFVMAADKVTPEAINFMATHGRGLICMPCAGWRLDQLRIPLMVGGRKGGQET
                     AFAVSIDHRYRTTTGTSAYDRSETIRAVTDPDSKPEDFDMPGHVFPLRAEEGGVLKRA
                     GHTEAAVDLAKLAGAYPAGVICEIMHPDGTMARLDELVRVAREHGLKLVSIADLIEFR
                     RRREALIRKVAEATIPTIYGEFKAQAYESLVDGRTHVAMVLGEMGDGGEVLVRVHSEC
                     LTGDVFHSLRCDCGTQLDDALGKIGSEGRGVLLYIRGHEGRAIGLTHKLRAYELQDRG
                     KDTVEANEVLGFAADPRDYGIGAQILHDLGVRTMRLLTNNPTKRAGLEGHGLRIVERV
                     PLETKPTEENIAYLRAKREKLGHLLSNLEAAEEGRA"
     gene            3257..3733
                     /gene="ribE"
                     /locus_tag="VG709_02240"
     CDS             3257..3733
                     /gene="ribE"
                     /locus_tag="VG709_02240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWC31929.1"
                     /translation="MTVHEGEISGRGRRIATVVSRFNDVVTSRLLEGALTALRRHGVA
                     DDDVVVAWTPGAFEIPLAAKALAASGEFDAVICLGAVVRGETAHFDYVAGEAASGVQR
                     VALETGVPCIFGVLTTDTLEQALDRAGGKHGNKGWEAASSALEMAGLLDALPKREA"
     gene            3738..4088
                     /locus_tag="VG709_02245"
     CDS             3738..4088
                     /locus_tag="VG709_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWC31930.1"
                     /translation="MIVDKPWGKVTTYALNQPSSVRVITIDPGEETSEHYHQLRDEMW
                     VVLDPGLKIQIGNRIVDASPGEEFVVQAEEPHRITNTAPQRGRVLEIAHGYTTEDDTF
                     RLQDDYGRPLEPRW"
     gene            4170..4373
                     /locus_tag="VG709_02250"
     CDS             4170..4373
                     /locus_tag="VG709_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWC31931.1"
                     /translation="MAQGTVKWFSNEKGYGFIARPEGEDVFVHYTAIAGDGYRSLAEG
                     QTVEFDIVDGPKGQQAANVRPVQ"
     gene            4582..5085
                     /gene="infC"
                     /locus_tag="VG709_02255"
     CDS             4582..5085
                     /gene="infC"
                     /locus_tag="VG709_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWC31932.1"
                     /translation="MGPDGAQVGIVSVEDALRRARDADLDLVEVAPQANPPVCRIMDY
                     GKFKYERAVRQKEARKKQARIEVKEIKFRPKIDRHDYETKKGHVVRFLNQGARVKVTI
                     MFRGREMAHTELGKRILDRLVEDLDGLATVDQPAKQDGRNMVMVIAPTKRPAKTEARE
                     GDREKVT"
     gene            5088..5282
                     /gene="rpmI"
                     /locus_tag="VG709_02260"
     CDS             5088..5282
                     /gene="rpmI"
                     /locus_tag="VG709_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWC31933.1"
                     /translation="MPRMKTHRGAAKRFRVTRTGKVLRRRAGLNHNLGKKDSSQKRRL
                     SRTAEVKRERDDVKRLLGKR"
     gene            5287..5646
                     /gene="rplT"
                     /locus_tag="VG709_02265"
     CDS             5287..5646
                     /gene="rplT"
                     /locus_tag="VG709_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWC31934.1"
                     /translation="MPRVKRSVHARKKRREILEQAKGYYGSRSRRFRVAREQLLHSGQ
                     YAYRDRRARKRDFRRLWITRIGAAARQNGMSYSELIRGLRAAEVEVDRKVLADLAARD
                     PRAFSGLVDLARRGLPA"
     gene            5663..>6123
                     /locus_tag="VG709_02270"
     CDS             5663..>6123
                     /locus_tag="VG709_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HWC31935.1"
                     /translation="MITSVRNRQVVAAARLQKRGIREQHRRFLVEGAQATEEALTAGA
                     VETLFHVPGATGRIDAIVRRAAEASVPLTAVSEAVMSHLTSTVTPQPIVAVARFIDAA
                     LEDLEIREGILPVLCFVRDPGNAGTILRTADAAGAAGVVFAEASVDVYNTKT"
BASE COUNT          999 a         2020 c         2264 g          840 t
ORIGIN      
        1 gtccacgtat ctgttcgacc gtcgctccga cgagggggag cgcatccccc ccggctgagg
       61 cggtcccgca cggccggcgg gcctatactt tggggagaag ctcgtcttcg gggcggggtg
      121 gaactcccca ccggcggtga cagtccgcga ccccctggga gccgctcagg ggtggacccg
      181 gtgaaactcc gggaccgacg gtaagagtcc ggatgggaga aggcgatgga gcggccggcg
      241 tctcgtgccg gcctgccctc ccgtccacgc cgctccaggc gagaacgaca cgaaaggtga
      301 gcgtggacga ggcgtacatg cggagggcgc tcgagctcgc ggcacgtggc cgcggcgtca
      361 cctctcccaa cccgctcgtc ggcgcggtcg tcgtgaagga cggccgtgtc gtggccgagg
      421 cgttccacga gggtgcgggt cgcaggcacg ccgaggcggt cgcgctcgac gccgcgggcg
      481 acgaggcgcg agacgccacg ctgtacgtca acctcgagcc ctgcgtgcac cacgggcgca
      541 cgccgccatg cgcgccgcga gtgatcgagt cgggtgtgtg gcgcgtcgtc gtcgcgatgc
      601 cggacccgaa cccgctcgtc gacgggcgcg ggttccgtgc cctgcgcgac gccggcatcg
      661 aggtcacgac cggcgtgctc gagcacgaat cgcgccggct gaacgaggcg ttcctgacgc
      721 acgtgcgcac tgggcgccct ttcgtcacgc tcaagatggc ggcgtcgctc gacggcaagg
      781 tcgcagcccg cgacggatcg tcccgctgga tcaccggcgg ggaggcgcgt gaagacgtcc
      841 accgcatgcg ggccgaggtc gacgcgatca tggtgggggc cgggacggcg ttccgcgacg
      901 atccttcact gacggttcgc gtcgccgggt accacggacg tgggaagctg cgtgtcctcg
      961 tcgacggacg cggcatcgtc ccggaaagcc accggctgtt cgccgagggc ggggcgccga
     1021 cgctcgtcgc cacgaccgag ggagcgcccg aggatcggcg ccgcgcctgg gccgacgccg
     1081 gcgccgacgt ggtcgtgatc gacgacgatg ggcgatcgct catcgagctc gacgcgctct
     1141 tccgcgagct cgggaagcgg gacgtgcagc acgtcctcct cgagggcggg ccgacgctcg
     1201 cgtgggaggc cgtccgccgc gacctcgtcg accgcgtcgt gctgttcttc gcgccgatct
     1261 tcgtgggagg cgatcgtgcc ccgagcgtgc tgatgggaga cgggacgccc acgatcggcg
     1321 aggcgcgccc cgtggcgatc gagtcggtcg agcgcgtggg agacgacatc aaggtggtgg
     1381 cgcgtgttca ccgggatcat tgaggagaga ggccgcgtga cggccgcccg gcggaccgac
     1441 ggcgtcgcgc aaatcgccgt caccggaccg aaggtcgccg aggacgcgga ggtcgggtcc
     1501 tccgtcgccg tgagcggcgt ctgcctcacg gtcgtcgagc ggagggggga cgggatgtcg
     1561 ttcgacgtcg tcgacgagac cctctcgcgg acgacgctgg gagatcttcg ggccggcgac
     1621 gtcgtgaacc tcgagcgccc cgtcacgctc gcgacgcggc tcggcggaca cctcgtgcag
     1681 gggcatgccg acgcggtagg aaccgttcga tcggtcgagt ggagcaactc cggctccgtg
     1741 atggcgatcg aggcagcccc cgacgttctg cgctacgtcg tcgagaaggg gtcggtcgcc
     1801 gtggacggcg tgagcctcac ggtcgcgcgt gcaggcggcg acgcgttcga cgtcgcgctg
     1861 gtcccgcaca cgctcgcggt cacgacgctc ggggagaggg acgccagcga tcgcgtgaac
     1921 ctggagatcg acatcgtggc gaagtacatc gaggggctca tcgggaggcc gggatgagcg
     1981 aggagcggac gaccgtggag gcgcgagccg acgagacgac ggtcgcggcg tcggcgttct
     2041 cgccgatcga ggacgccatc gaggacgtcc gcaggggccg catcgtgatc gtcgtggacg
     2101 acgccgatcg cgagaacgag ggcgacttcg tcatggcggc cgacaaggtg acccccgagg
     2161 cgatcaactt catggcgacg cacgggcgcg gcctgatctg tatgccgtgc gccggctggc
     2221 ggctcgacca gctcaggatc ccgctcatgg tcggcggacg gaagggcggc caggagacgg
     2281 ccttcgcggt ctcgatcgat caccggtaca ggacgaccac cggcacgagc gcctacgacc
     2341 gctcggagac catccgtgcc gtgaccgacc ccgactcgaa gcccgaggac ttcgacatgc
     2401 cgggacacgt gttcccgctc cgcgccgagg agggcggcgt gctcaagcgc gcgggacata
     2461 ccgaggcggc ggtcgacctc gcgaagctcg ccggcgcata cccggccggt gtcatctgcg
     2521 agatcatgca tcccgacggg acgatggctc ggctcgacga gctcgtccgc gtcgcccgcg
     2581 agcacggcct gaagctcgtc tcgatcgccg acctgatcga gttccgccgg cggcgtgagg
     2641 cgctgatccg caaggtcgcc gaggcgacga tcccgaccat ctacggcgag ttcaaggcgc
     2701 aggcgtacga gagcctcgtc gacggccgga cgcacgtcgc gatggtcctc ggcgagatgg
     2761 gcgacggcgg cgaggtactc gtccgcgtcc actccgagtg cctcacgggc gatgtgttcc
     2821 actcgctgcg ctgcgattgc ggcacccagc tcgatgacgc gctcgggaag atcggctcgg
     2881 agggacgcgg cgtgctgctc tatatccgcg gccacgaggg gcgcgcgatc gggctcacgc
     2941 acaagctgcg cgcgtacgag ctgcaggacc gcggcaagga caccgtcgag gcgaacgagg
     3001 tgctcggctt cgccgccgat ccccgcgact acggcatcgg cgcgcagatc ctccacgacc
     3061 tcggggtccg aacgatgcgg ctcctcacga acaacccaac gaagcgggcg gggctggagg
     3121 gccacgggct tcggatcgtg gagcgcgtcc cgctcgagac caagccgacc gaggagaaca
     3181 tcgcgtacct gcgcgcgaag cgcgagaagc tcgggcacct gctctcgaac ctcgaggcgg
     3241 cggaggaggg aagggcgtga cggtccacga aggggagatc tcggggcgcg gccgccggat
     3301 cgcgaccgtc gtctcgcggt tcaacgacgt cgtcacctcg cggctcctgg agggcgcgct
     3361 cacggcgctc cggcgccacg gcgttgcgga cgacgacgtc gtcgtcgcat ggacgccggg
     3421 cgccttcgag atcccgctcg cggccaaggc cctcgcagcg tcgggagagt tcgacgccgt
     3481 catctgtctc ggcgccgtcg tccgcggcga gacggcgcac ttcgactacg tcgcgggcga
     3541 ggcggcatcg ggcgtgcagc gcgtcgcgct cgagaccggg gtgccgtgca tcttcggcgt
     3601 gctcacgacc gacacgctcg agcaggcgct ggaccgcgcc ggcggaaagc acgggaacaa
     3661 gggatgggag gcagcgtcga gcgctctcga gatggccgga ctcctcgatg cgctcccgaa
     3721 gcgggaggcg tgaagcgatg atcgtcgaca agccgtgggg gaaggtcacg acgtacgcgc
     3781 tgaaccagcc gtcgtctgtg cgcgtgatca cgatcgatcc gggcgaggag acgagcgagc
     3841 actaccacca gctccgtgac gagatgtggg tcgtactcga tccgggcctg aagatccaga
     3901 tcggcaaccg catcgtcgac gcgtcaccgg gcgaggagtt cgtcgtgcag gccgaggagc
     3961 cgcatcgcat cacgaacacc gcacctcaac ggggccgcgt cctcgagatc gctcatggct
     4021 acacgacgga ggacgacacg ttccggttgc aggacgacta cggccgcccg ctcgaaccgc
     4081 gctggtgact cgtacgcgaa cacgcgtggt ggtacgctcc ccgttcgtca tggcagggcg
     4141 gcgtcgccgc cgaagcaagg agggctgggt tggctcaagg aacggtcaag tggttcagca
     4201 acgagaaggg gtacggcttc atcgcccgcc ctgagggaga agacgtcttc gtgcactaca
     4261 cggcgatcgc tggcgacggc tatcgcagct tggccgaggg ccagacggtg gagttcgaca
     4321 tcgtcgacgg tccgaagggc cagcaggcgg cgaacgtccg gccggtccag tagctgagcg
     4381 acctcacgag gaggggcctt tgggcccctc ctcgcgtatc gacagccgcc tcgccgtcgc
     4441 catatactcg acccggatga gcacggcttt ccctgagcgc cgcacgtgac gcacctatct
     4501 cggcgtgccc cccggaggtg acctcgagtc tcggttgatc cacgcataaa cgagcgcatc
     4561 cgcgcgccac aggtccgcct cgtgggaccg gacggcgcgc aggtggggat cgtctcggtc
     4621 gaggacgcgc tccgccgggc gcgcgacgcg gacctcgacc tcgtcgaggt ggccccgcag
     4681 gcgaacccgc cggtctgccg catcatggat tacggcaagt tcaaatacga gcgagccgtg
     4741 cggcagaagg aggcccgtaa gaagcaggcg cggatcgagg tcaaggagat caagttcagg
     4801 ccgaagatcg accgccacga ctacgagacg aagaaggggc acgtcgtccg gttcctgaac
     4861 cagggcgcga gggtgaaggt gacgatcatg ttccgtgggc gtgagatggc ccatacggag
     4921 ctggggaagc gtatcctcga tcggctcgtc gaggacctcg acgggctggc gacggtcgac
     4981 cagccagcga agcaggacgg tcgcaacatg gtgatggtga tcgcacccac gaagcgcccg
     5041 gctaagaccg aggcgcgcga gggcgatcga gagaaggtga cgtagcgatg ccgaggatga
     5101 agacgcacag gggtgcggcg aagcgcttcc gcgtgacgag gaccggcaag gtcctccggc
     5161 ggagggcggg actcaaccac aacctcggga agaaggactc gagccagaag cgacggctct
     5221 cgcgcaccgc cgaggtcaag agggagcgcg acgacgtgaa gcggctcctg gggaagcggt
     5281 agcgcgatgc cgcgcgtcaa gcgctccgtc catgcgcgca agaagcggcg cgagatcctg
     5341 gagcaggcca aggggtatta cggctcgcgc tcacggcggt tccgggtcgc gcgcgagcag
     5401 ctgctgcact ccgggcagta cgcgtaccgc gaccgccgtg cgcgcaagcg ggacttccgg
     5461 cgtctgtgga tcacccggat cggcgccgcc gccaggcaga acggcatgtc gtactccgag
     5521 ctgatccggg gcctccgcgc cgcagaggtc gaggtcgacc ggaaggtcct cgcagacctg
     5581 gcggcgcgcg atccgcgtgc gttctcggga ctcgtagacc tcgcccggcg cggtctcccc
     5641 gcctgacgcg gacctcgacc ccgtgatcac gagcgtccgg aaccgccagg tcgtcgcggc
     5701 cgctcggctc cagaagcgcg ggattcgcga gcagcatcgc cgtttcctcg tcgagggggc
     5761 gcaggccacg gaggaggcgc tcacggcggg agcggtcgag acgctgttcc acgtcccggg
     5821 tgccaccggg cgcatcgatg cgatcgtccg tcgagcggcg gaagcgagcg ttccgctcac
     5881 ggcggtgtcc gaggcggtga tgtctcacct cacctcgacc gtgacgccgc agccgatcgt
     5941 cgccgtcgcg cgcttcatcg acgctgcgct cgaggatctc gagatccgcg aagggatctt
     6001 gccggtgctg tgcttcgtcc gcgatcccgg gaacgccggg acgatccttc ggaccgccga
     6061 cgccgccggc gcggccggcg tcgtgttcgc ggaggcgtcg gtggacgtct acaacaccaa
     6121 gac
//
LOCUS       DASZOB010000140         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_63901, whole genome shotgun sequence.
ACCESSION   DASZOB010000140 DASZOB010000000
VERSION     DASZOB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63901"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..639)
                     /locus_tag="VG709_02275"
     CDS             complement(16..639)
                     /locus_tag="VG709_02275"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWC31936.1"
                     /translation="MRLAGFLETLVGWIGPIFASAGYWIVSGAVTLERSIFIGLVVPG
                     DVILAMGGIYSARGHLEIEWVIALGTIAGIVGESAGYWLGRRYGVTLVGGIPLIRRLE
                     KRLNEAKKYFEEHGGKTVAIGRYASAIGALIPFVAGIARMPYAKFLAFDVPAVVVWAV
                     GFSLVGYFFGANLETVDSILSRFGWIMFGVLVVLVGAVVVARRRRRR"
     gene            complement(658..1662)
                     /locus_tag="VG709_02280"
     CDS             complement(658..1662)
                     /locus_tag="VG709_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013879094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWC31937.1"
                     /translation="MSTVNGRRRVAVVTGASAGVGRATARELAQRGADVALVARGDDG
                     LRAAAREVESFGRSALVVRADVSDAEAVEEAAARTERDLGPIDVWINAAMTSVFSPFT
                     EMEHDEFRRVTEVTYLGYVNGTRAALRRMKSRDRGTIVQVGSALAYRGIPLQSAYCGA
                     KHAIQGFTESVRTELLHDGSRVRLIEVHLPALNTPQFDVVKSHLPRRAQPVPPIYQPE
                     VAAGAIAWASEHGDWEVHVGGTTVATILANKVAPRLLDRYLGRTGFDSQQTDEPEDPE
                     RPYNLWEPVSGDHGAHGRFDDRSKPRSVQWWLNERRRVLVAVAAVGAAAVLAARGRSR
                     "
     gene            1738..2631
                     /locus_tag="VG709_02285"
     CDS             1738..2631
                     /locus_tag="VG709_02285"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWC31938.1"
                     /translation="MEGTWFVLPTAFDDAGALDLDAQQTLVDRVVAWGVDGLVTMGVT
                     SEAAALTDPERDHVVRAVVDVAARRVPVVVGCSGASVEIVAERASRASALGGAAALVS
                     APPAARDVDALPRFFERAATVSEVPLVVQDEPAATGTTIPTRVLVRCIDAARADTVKL
                     EDPPTPPKVADVVEARPDVAVFGGLGGLYALDELRRGGRGTMTGFAFPEILAKVRRLV
                     AAGDAAAATVFDSHLPLIAFEAQQRIGLAIRKEVLRRRGALRSAATRLAPELDAGTVR
                     ALDDVLRRVGIVPGSERLELG"
     gene            2636..>3141
                     /locus_tag="VG709_02290"
     CDS             2636..>3141
                     /locus_tag="VG709_02290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC31939.1"
                     /translation="MAERVDVAVSGGGAVGLSGARELRLASVDRVVVLEREPSVGQGS
                     SARANGGVRAQFTTPINIAFSLHSIVEFESLRDRYGEVLSFHQTGYLFLTGTEEGELG
                     LRSAMELQRSLGVDTRWIEPAEVPALAPIVAVDGLRGATFHRRDGFLDPHGTVEVMRL
                     ECRRLGAEV"
BASE COUNT          449 a         1135 c         1103 g          454 t
ORIGIN      
        1 cgcggaggcc gccctctaac gtcgccgccg gcggcgggcg accacgacgg cgcccacgag
       61 aacgaccagg accccgaaca tgatccaccc gaaccgggac aggatcgagt ccaccgtctc
      121 gaggttcgcc ccgaagaagt atccgaccag cgagaacccg accgcccaca ccacgactgc
      181 cgggacgtcg aaggcaagga acttcgcgta cggcatcctc gcgatgccag cgacgaacgg
      241 gatcagcgcg ccgatagcgc tcgcgtaccg tccgatagcg accgtctttc ctccgtgctc
      301 ctcgaagtac ttcttggcct cgttgagcct cttctcgagg cgccggatga gcgggatgcc
      361 tccgaccaac gtgacgccgt accgtctccc gagccagtag cccgccgact cgccgacgat
      421 gccggcgatc gtgccgagcg cgatcaccca ctcgatctcg aggtgcccgc gcgccgagta
      481 aatgccaccc atcgcgagga tcacgtctcc ggggacgacg agaccgatga agatcgaccg
      541 ctcgagcgtc acggctcccg aaacgatcca gtaaccggcg ctggcgaaga tcgggccgat
      601 ccaaccgacg agcgtctcga ggaagccggc gagcctcacg atcgcggggg gccaagccta
      661 acgagagcga cctcgtgccg caagaacggc ggcggcgccg acagccgcga ccgcgacgag
      721 gacgcgtcgg cgctcgttaa gccaccactg cacgctgcgc ggcttcgagc ggtcgtcgaa
      781 gcgcccgtgc gcgccgtggt cgccggagac gggctcccag aggttgtacg gacgctccgg
      841 gtcctccggc tcgtcggtct gctgcgagtc gaaaccggtc ctgccgaggt agcggtcgag
      901 gagccgcggc gcgaccttgt tcgccaggat cgtcgcgacc gtcgtgcccc cgacgtgcac
      961 ctcccagtcg ccatgctcgc tcgcccacgc tatcgcgccg gccgcgacct cgggctggta
     1021 gatcggcggg accggctgcg cgcgcctcgg gaggtgcgac ttcacgacgt cgaactgcgg
     1081 cgtgttgagc gcgggcaggt gcacctcgat gagccggacc cggctcccgt cgtggagcag
     1141 ctcggtcctc accgactccg tgaagccctg gatcgcgtgc ttggcgccgc agtacgcgct
     1201 ctggagaggg atcccgcggt acgcgagcgc cgatccgacc tgaacgatcg tgccgcggtc
     1261 ccgcgacttc atgcgacgca gcgccgcgcg cgtgccgttc acgtagccga ggtacgtgac
     1321 ctctgtgacc cgccggaact cgtcgtgctc catctcggtg aacggcgaga acaccgacgt
     1381 catcgccgcg ttgatccaga cgtcgatcgg gccgaggtcg cgctcggtcc tcgcggcagc
     1441 ctcctcgacg gcctcggcgt ccgacacgtc ggcacgcacg acgagggccg agcggccgaa
     1501 agactccacc tcgcgggcgg cggcccggag cccgtcatca ccgcgcgcca ccagggcaac
     1561 gtcggcgccg cgctgcgcga gctccctcgc cgtcgcgcga ccgacgcccg ccgaggcacc
     1621 ggtgacgacg gcgacacgcc tgcggccgtt cactgtgctc atgctccgcc tccttccggt
     1681 gcttcgttcc cgagggcgcg gcgcacaaac agacgtctgt ccggtagcgt tccggtcgtg
     1741 gagggcacgt ggttcgtgct tccgacggcc ttcgacgacg ccggggcgct cgacctcgac
     1801 gcacaacaga cgctcgtcga ccgcgtcgtg gcgtggggcg tcgacggcct ggtgacgatg
     1861 ggcgtcacga gcgaggcggc cgcactcacg gaccccgaac gcgaccacgt cgtccgggcg
     1921 gtcgtggacg tcgcggcgcg gcgcgtcccg gtcgtcgtcg gatgctccgg cgcctcggtc
     1981 gagatcgtcg ccgagcgcgc cagccgagcc tcggcgctcg gtggcgcggc ggcgttggtc
     2041 tcggcgccac ccgcggcgcg agacgtcgat gcgctgccgc ggttcttcga gcgcgccgcg
     2101 acggtctccg aggttccgct cgtggtgcag gacgaacctg ccgcgaccgg aacgacgatc
     2161 cccacgcgcg ttctcgtccg gtgcatcgat gcggcccgcg cggacaccgt caagctcgag
     2221 gatcctccga cgcctccgaa ggttgcggac gtcgtcgagg cgagaccgga cgttgccgtg
     2281 ttcggcggcc tcggcggcct ctacgcgctc gacgagctgc gccggggcgg tcgcgggacg
     2341 atgacggggt tcgctttccc tgagatcctg gccaaggtgc gccggctggt ggccgcaggc
     2401 gacgcggccg cggcgaccgt cttcgacagc cacctgccgc tgatcgcatt cgaggcgcaa
     2461 cagcgcatcg ggctcgcgat ccgcaaggag gttcttcggc gccgcggcgc gctgcgctcg
     2521 gcggcgacgc gtctcgctcc ggagctcgac gcgggaaccg tccgcgcgct cgacgacgtc
     2581 ctacgacggg tcgggatcgt cccgggctcg gagcgcctcg agctcgggta gccgcgtggc
     2641 cgagcgcgtc gacgtcgcgg tcagcggagg cggcgccgtc gggctgtccg gcgcgcgcga
     2701 gctccggctt gcgagcgtcg atcgcgtcgt cgtcctcgag cgcgaaccgt ccgtcggaca
     2761 gggttcgtcc gcgcgcgcga acggcggcgt gcgcgcacag ttcacgaccc cgatcaacat
     2821 cgcgttctcg ctccactcga tcgtggagtt cgagagtctc cgcgatcgtt acggggaggt
     2881 gctctcgttc caccagaccg gctatctgtt cctgaccggc acggaagagg gtgagctggg
     2941 gctccggagc gcgatggagc tgcagcgctc gctcggcgtc gacacgcggt ggatcgagcc
     3001 ggcggaggtg ccggccctcg cgccgatcgt cgccgtcgac ggcctccgtg gagcgacgtt
     3061 ccaccgacgc gatgggttcc tcgacccgca cggcaccgtc gaggtgatgc ggctggagtg
     3121 ccggcgtctc ggcgccgagg t
//
LOCUS       DASZOB010000141         4687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_11657, whole genome shotgun sequence.
ACCESSION   DASZOB010000141 DASZOB010000000
VERSION     DASZOB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11657"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..120
                     /locus_tag="VG709_02295"
     tRNA            38..120
                     /locus_tag="VG709_02295"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Tyr,seq:gta)
     gene            complement(170..853)
                     /locus_tag="VG709_02300"
     CDS             complement(170..853)
                     /locus_tag="VG709_02300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWC31940.1"
                     /translation="MKRANRETFRSGRSRPHVVLVRHGETEWSRDGRHTGRTDVPLTE
                     VGRREARMLAARLRVWRFAEIVSSPLRRALETCRLAGYGDVVEVRDELLEWDYGDYEG
                     RTTADIRTEHPGWTIWAAGAPGGERAEDVGARVDPLVAELREAQGDVAVFGHGHLLRV
                     LAARWLDLPAHQGRAFALGTTTVSVLGFERETPVILRWNEDCHLGPHGAQVLAPESAT
                     EGGADLADD"
     gene            complement(918..1157)
                     /locus_tag="VG709_02305"
     CDS             complement(918..1157)
                     /locus_tag="VG709_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWC31941.1"
                     /translation="MQLEPRDTSAVAPGLPRVGVPARVVWGADDRFQKLRFGERLARD
                     LGVAVDRIEGGKHFTREDHLDRIAAAIGDLLADVR"
     gene            complement(1332..2726)
                     /gene="sthA"
                     /locus_tag="VG709_02310"
     CDS             complement(1332..2726)
                     /gene="sthA"
                     /locus_tag="VG709_02310"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWC31942.1"
                     /translation="MTERFDLVVIGAGPAGEKAAAQAAYFGKRVAVVDRLPHAGGSVV
                     ASAGIPTKTLRETALYITGFRSREVYGLSLHLDADTTLERLMARKTDVVERMAASVDA
                     NLRRHGITFIRGEGRVDERARVHVSADGEERTLEAEIVLLATGSRPFRPPGIPFEDPD
                     VHDSEEVLQIQRIPESLVVVGGGPVGCEYAAIFTALGTNVTLLDAADRLVPFADGEIS
                     ALLVETFEAMGMRVVVGSGVATIERTGDGLAVRLASGDVLHPDQVLFAAGRTGNTEGL
                     GLDEAGVALDARGRIVVDDTFRTSVERIYAAGDVIGPPALASVSMEQGRVAVCPAFGI
                     PFKDTVDPLAPFAVYSIPELAMVGLTEEAAAAEGIEYEVGRSPFASNTKAAITAFPDG
                     LVKLVFRRDDRRLLGVHILGEGASELVHVGQATIRSGETIDGFIHATFATPTRSEAYK
                     YAAYDGLQRLSGRS"
     gene            complement(2723..3136)
                     /locus_tag="VG709_02315"
     CDS             complement(2723..3136)
                     /locus_tag="VG709_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWC31943.1"
                     /translation="MRDQPLSYARLGADPIRTISRDELKAKLDRGDPIKLIMALNRWA
                     FDAKHIPGSLHFDDPEELYRAVRPDDEVIVYCSNVDCLSSVALYRDLVGRGYRNVRRY
                     AGGLIDWEDASLPLEGGLAGGGPGPRDIADTGEPT"
     gene            3184..3891
                     /locus_tag="VG709_02320"
     CDS             3184..3891
                     /locus_tag="VG709_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31944.1"
                     /translation="MSEQQLYRLGAISGIVGGVLALVFNLLHPRLGDFSDPVSEDLRV
                     AAQSDAWTLIHVGVTAGVLLLIAALYVVARSLREDGAGYRLGRLALGSLVISAPVGLV
                     TLAIDGYATKYVADLVAGGGPLAAGQAVAAIGWAVFMFFVIMLLGVTPALFGAAIAAS
                     DDYPAVLGWPAVVLGFGSIVAGLIGYFGGPSAGFFLVFMITSGLLTVWVIALGVVLWR
                     RSADVPDRAATPVRPTA"
     gene            complement(3903..4394)
                     /locus_tag="VG709_02325"
     CDS             complement(3903..4394)
                     /locus_tag="VG709_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWC31945.1"
                     /translation="MIEAMNQRRRPTALLLLIGLAAVALAACADEPEEPQRTLERGTM
                     TIVTEEGPVRLDVEIAETDREKQTGLMHRESLGERSGMVFLEEQPVRIAFTMRNTRIP
                     LSLAVWGPDGRIRSIIDMDPCRSEPCPSYDPGVAWVGALEVNQGFFEEHGVEVGDVVR
                     LEH"
     gene            4406..4478
                     /locus_tag="VG709_02330"
     tRNA            4406..4478
                     /locus_tag="VG709_02330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4439..4441,aa:Thr,seq:ggt)
     gene            4505..4576
                     /locus_tag="VG709_02335"
     tRNA            4505..4576
                     /locus_tag="VG709_02335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4537..4539,aa:Met,seq:cat)
BASE COUNT          662 a         1659 c         1594 g          772 t
ORIGIN      
        1 ttcgccatga ggccgtactg taatctagag atggtccggg ggggtgcccg agcggccaaa
       61 gggagcggtc tgtaaaatcg ccggcgtagc ctacgcaggt tcgaatcctg cccccccact
      121 cacactcact cactcatcgc acacaaacga gacacacaca ctcactgagt cagtcatcag
      181 ccaagtcagc cccgccttcg gtcgcgctct ccggggcgag cacctgcgcg ccgtgcggcc
      241 ctagatggca gtcctcgttc caccggagaa tcaccggggt ctcccgctcg aagcccagca
      301 cgctcaccgt ggtcgtcccg agggcgaacg ctcgcccctg atgtgcaggg aggtcgagcc
      361 accgggctgc gagcacccgg aggaggtgcc cgtgaccgaa cacggccacg tccccttggg
      421 cctcgcggag ctcggccact aggggatcga ctcgcgcccc gacgtcctcg gcgcgttcgc
      481 cacctggggc cccggcagcc cagatcgtcc agcccgggtg ctcggttcgg atgtcggccg
      541 tggtccggcc ctcgtagtcc ccgtagtccc attccaggag ctcgtcgcgg acctccacca
      601 cgtccccgta cccggcgagg cggcaggtct ccagggctct ccggagcggg ctcgagacga
      661 tctcggcgaa ccgccacacc cgtagccggg cggcgagcat cctcgcctcc cgccgtccaa
      721 cctcggtgag cgggacgtcg gtgcgcccgg tgtggcggcc gtcgcggctc cactcggtct
      781 ccccgtggcg gaccaggacg acgtgcggtc tgcttctccc tgaccggaat gtctcgcggt
      841 tcgctcgctt caccgtgtca ccgggtcggg tcgaagtccc ctcgctgagg aacggcgtac
      901 tcggctgcgc cgtctcgtca ccgcacgtcc gcgaggaggt cgccgatcgc cgccgcaatc
      961 cgatcgagat ggtcctcgcg ggtgaagtgc ttcccgccct cgatgcggtc caccgccacg
     1021 ccgaggtcgc gggcgaggcg ttctccgaat ctcagcttct ggaaccggtc gtcggctccc
     1081 cagacgacgc gggccgggac gccgacgcgg ggaaggccgg gagcgaccgc cgacgtgtcg
     1141 cggggctcga gctgcacgct gttgcgtgac atacccgcac cgtgcgggcg ctagcgggcc
     1201 catcggtcct gttactgcac gggtaggcgg tacgcccggt ctcgagtaag gaggagtgag
     1261 cttccgcggt cctcgcgaag ccgtgccgat gtgtggggtg gcgtacgtgg ctgaaggccc
     1321 ctcgagacga tctagcttcg cccgctcaaa cgctggaggc cgtcgtacgc cgcgtacttg
     1381 taggcctcgc tcctcgtcgg cgttgcgaac gtcgcatgga tgaagccgtc gatcgtctcg
     1441 cccgagcgga tcgtcgcttg cccgacgtgg acgagctcgg acgcgccctc cccgaggatg
     1501 tgcacgccga ggagcctgcg gtcgtcgcgc cggaacacca gcttcacgag cccgtccggg
     1561 aacgcggtga tcgctgcctt cgtgttagac gcgaacgggc tccgaccgac ctcgtactcg
     1621 atgccttcgg ccgccgccgc ctcttccgtg aggccgacca tcgcgagctc ggggatcgag
     1681 tagaccgcga acggcgccag cgggtcgacg gtgtccttga acgggatccc gaacgcgggg
     1741 cacacggcga cgcgcccctg ctccatcgag accgacgcga gcgcaggcgg gccgatcaca
     1801 tcgcccgcgg cgtagatccg ctcgaccgac gtacggaagg tgtcgtccac cacgatccgg
     1861 cctcgagcgt cgagggcgac gcctgcctcg tcgagcccga gaccctcggt gttcccggtg
     1921 cgccccgcgg cgaacagcac ctggtccgga tgaaggacgt cgccgctcgc gaggcgcacc
     1981 gcgagtccgt ccccggtgcg ctcgatcgtg gcgactccgg aaccgacgac gacgcgcatc
     2041 cccatcgcct cgaacgtctc gacgagtagc gccgagatct ctccgtccgc gaacgggacg
     2101 aggcggtcgg cggcgtccag cagcgtgacg ttcgtcccga gagcggtgaa gatcgcggcg
     2161 tactcgcatc cgacggggcc gcccccgacc acgacgagtg actccgggat ccgttggatc
     2221 tgcaagacct cttcggagtc gtggacgtcc gggtcttcga atgggatgcc gggaggtcgg
     2281 aacggccgcg aaccggtcgc gaggagcact atctccgctt cgagcgttcg ctcctcgccg
     2341 tcggccgaga cgtgcaccct ggccctctcg tcgacccgtc cctctccacg gatgaacgtg
     2401 atgccgtggc gccggaggtt cgcatcgacg gacgccgcca tccgctccac gacgtcggtc
     2461 tttcgcgcca tcagccgctc gagcgtggtg tcggcatcga ggtgcaggct gaggccgtag
     2521 acctcgcgac tgcggaaacc cgtgatgtag agagccgttt ctcgcagcgt cttggtcggg
     2581 atccctgcgg acgccaccac ggatcctccg gcgtgcggga ggcggtcgac gaccgcgacc
     2641 cgcttcccga agtaagcggc ctgggcggcg gccttctctc cggccgggcc ggcaccgatg
     2701 accaccaggt cgaaacgctc ggtcacgtcg gctcgcccgt gtcagcgatg tctctcgggc
     2761 cgggtccacc gcccgcgagg ccgccctcga gcggcaggct ggcgtcctcc cagtcgatga
     2821 ggccgcccgc gtaccggcgg acgttgcggt agccgcgtcc gacgaggtct cggtagagcg
     2881 cgacgctcga gaggcagtcg acgttcgagc agtagacgat cacctcgtcg tccggacgga
     2941 cggctcggta gagctcctcg ggatcgtcga agtggagcga ccccggaatg tgtttcgcgt
     3001 cgaacgccca gcggttgagc gccatgatca gcttgatggg gtcgccgcga tcgagcttcg
     3061 ccttcagctc gtcacgcgag atcgtccgga tcgggtcggc gccgagccgc gcataggaga
     3121 ggggttgatc gcgcacgtct cgtccagcct aaactccccc cgtccagcga ggaggaggcc
     3181 cgcatgtcgg agcagcagct ctaccggctc ggagcgatca gcgggatcgt cggaggcgtg
     3241 ctcgccctcg tcttcaacct gctccatcca cggctcggcg acttctccga tccggtgagc
     3301 gaggacctgc gcgtcgccgc gcagagcgac gcgtggacgc tcatccacgt cggggtcacc
     3361 gccggggtgc ttctcttgat cgccgccctc tacgtggtgg cgcggtcgct ccgcgaggac
     3421 ggggcaggct accgtctcgg ccggctcgcg ctcgggagcc tcgtgatctc ggcgccggtc
     3481 gggctcgtga cgctcgcgat cgacgggtac gcgacgaagt acgtcgcgga cctcgttgcg
     3541 ggtggcggac cgctcgctgc ggggcaggcc gtggccgcga tcggatgggc cgtcttcatg
     3601 ttcttcgtga tcatgctgct gggggtgacg ccggcgctct tcggcgccgc gatcgccgca
     3661 tccgacgact acccggccgt gctcggttgg cctgcggtcg tcctggggtt cgggtcgatc
     3721 gtggccggcc tcatcggcta cttcggcggg ccgtcggcgg ggttcttcct ggtgttcatg
     3781 atcacgtcgg ggctcctcac ggtctgggtc atcgcgctcg gcgtggtgct ctggcggcgt
     3841 tcggccgacg tgccggatcg ggccgcgacg ccggtccgcc cgacggcctg actcgccgcg
     3901 agtcagtgct cgaggcgaac gacgtctccg acctcgacgc cgtgctcctc gaagaacccc
     3961 tggttgacct ccagcgcgcc gacccacgcg acgcccgggt cgtaggacgg acacggctct
     4021 gaccggcacg gatccatgtc gatgatcgag cggatccggc cgtcgggccc ccagaccgcg
     4081 agcgagagcg ggatccgcgt gttgcgcatc gtgaacgcga tgcgtaccgg ctgctcctcg
     4141 aggaagacca tccccgagcg ctctccgagg ctctcccggt gcatcagccc ggtctgcttc
     4201 tcgcggtcgg tctccgcgat ctcgacgtcg agcctcacgg gaccctcctc ggtcacgatc
     4261 gtcatcgtgc cgcgctcgag cgtccgctgc ggctcctcgg gctcgtccgc gcacgccgcg
     4321 agcgccacgg ccgcgagccc gatgagaagg agtagcgccg tcggtcgtcg tcgctggttc
     4381 atcgcctcta tcatgggctc ctagcgctgg agtagctcag ccggcagagc acctccatgg
     4441 taaggagggg gtcgtgggtt cgagtcccac ctccagctcg gacccgcgac tcggtcgcgg
     4501 tcgcggcggg gtagctcagt ggcagagcaa gcggctcata atcgctgtgt cggcggttcg
     4561 aatccgcccc ccgctactcg acgcctccgg tgctcgacgt tagcgcggac gcgtccgccc
     4621 cttctcggta cacttcgtcg gccatgagca agaagaagtt tgagcgcacg aagccgcacg
     4681 tcaacat
//
LOCUS       DASZOB010000142         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_144139, whole genome shotgun sequence.
ACCESSION   DASZOB010000142 DASZOB010000000
VERSION     DASZOB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144139"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1136)
                     /locus_tag="VG709_02340"
     CDS             complement(63..1136)
                     /locus_tag="VG709_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWC31946.1"
                     /translation="MSATLPERLGEEIRRDGPVTFARFMEVALYDPADGFYARPPVGA
                     EGHFLTSPHVAPVFAWLLARQIGEAFDLLGSPERFDVVEHGAGDGTLARQLTEALGAV
                     PALARALRYVAVERSSGALDALPSAGIGAVEPAAAPRETEGVCFANELLDNVPFHLLR
                     RDGSALRELFVTATDEEFAFEEGPPSNARLAELAASLADGAIGAVSPRSVDWLLDAAS
                     RMRRGYLLVIDYGGAGPRPPQTYRAHRAATELLRDPGSADITAGVDVEALAAAAREAG
                     LRVWGPVSQRDALMRLGYGEYAGAERRRTAAEREAGSPLAAARRWARRGRESMLADPG
                     ALGSLFWLVIGVGVDEPLACVRD"
     gene            complement(1144..>1801)
                     /locus_tag="VG709_02345"
     CDS             complement(1144..>1801)
                     /locus_tag="VG709_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31947.1"
                     /translation="TPRGPGRATTSVAAGRGSCTGRLWQHGGVRRPTAGARRVTTAVV
                     ALIALGACGGDVDVTIGSPQAGPSPTDDPVVGPTALPSIPGLPDIDLGDLTQLTDGEA
                     SVEVSGDVESSIDASLSVGSGVAEDASAFNVVYTDANQNTVTITGAFDDVSDGPQTVV
                     NIALSRPSFVLFTGTDCNVSVERTANGLEGSFDCDGLTSSLTGESKTIDAEGEFTAST
                     "
BASE COUNT          242 a          657 c          663 g          239 t
ORIGIN      
        1 tcgcgatcct cggctcgatc ttcttcgtcg tcggcgacgt cgacaagtag tcgggccgcg
       61 actcagtccc ggacgcaggc gagcggctcg tcgacgccga cgccgatcac gagccagaac
      121 aggctcccca gcgcaccggg gtcggccagc atcgactccc ggccgcgtcg ggcccaccgg
      181 cgcgcggcgg cgagggggga gccggcctcg cgctccgcgg cggtccgtct tcgctcggcg
      241 cctgcgtact cgccgtagcc cagccgcatc agggcatccc gctgcgagac cgggccccag
      301 acccgcaggc cggcctcgcg cgcggccgcg gcgagcgctt ctacgtcgac accggcggtg
      361 atgtccgcag agcccggatc gcgcaggagc tccgtggcgg cgcggtgcgc ccggtaggtc
      421 tgcggtgggc gcgggccggc cccgccgtag tcgatcacga ggaggtagcc gcggcgcatc
      481 cgtgaggccg cgtccagcag ccagtcgacg gaccggggcg agaccgcgcc gatcgctccg
      541 tcggcgagcg acgccgcgag ctccgcgagt cgcgcgttcg acggcgggcc ctcctcgaag
      601 gcgaactcct cgtcggtcgc cgtgacgaac agctcgcgga gcgcggaacc gtcgcggcgg
      661 agcaggtgga acggcacgtt gtcgagcagc tcgttcgcga agcagacgcc ctcggtctcg
      721 cgcggggcgg cggcgggctc cacggcgccg atccccgcgg agggcagcgc gtcgagcgcg
      781 ccggaggagc gctccacggc gacgtagcgc aacgctcgag cgagcgccgg gacggcgccc
      841 agcgcctccg tgagctggcg cgccagcgtg ccgtcgccgg cgccgtgctc gacgacgtcg
      901 aaccgctcgg gcgagccgag caggtcgaac gcctccccga tctgccgggc gagcagccac
      961 gcgaagaccg gggcgacgtg cgggctcgtg aggaagtggc cctccgcgcc gaccggcggc
     1021 cgagcgtaga acccgtcggc cgggtcgtag agcgcgacct ccatgaagcg ggcgaacgtg
     1081 accggcccgt cgcggcggat ctcctcgccg agccgctcgg ggagcgttgc gctcacgcgt
     1141 cgctcaggtg gaggccgtga actcgccctc ggcgtcgatc gtcttcgact cgcctgtgag
     1201 tgagctcgtc aggccgtcgc aatcgaagct gccttcgagc ccgttcgcgg tccgctccac
     1261 ggagacgttg caatccgtgc ccgtgaagag cacgaagcta ggacgtgaca acgcgatgtt
     1321 cacgaccgtc tgagggccgt cgctgacatc atcgaatgcc ccggtgatcg tcacggtgtt
     1381 ctggttcgcg tccgtgtaga cgacgttgaa cgcgctcgcg tcctcggcga ctcctgatcc
     1441 aactgagagc gatgcgtcga tcgagctctc gacgtcgccg ctcacctcga cgctcgcctc
     1501 cccgtcggtc agctgcgtga ggtcgccgag atcgatgtcg ggcaggccgg ggatcgaggg
     1561 gagcgcggtc ggtccgacga ccggatcgtc ggtcggtgac ggccccgcct ggggactccc
     1621 gatggtcacg tcgacgtcgc cgccgcaggc gccgagcgcg atgagtgcga cgacggcggt
     1681 cgtcacgcgc cgggctccag cggtcggacg tcgcacgccg ccatgctgcc acaatcgccc
     1741 cgtgcaggag ccgcgtcccg ccgccacgct cgtcgtcgcg cgtcccggcc cgcgcggcgt
     1801 g
//
LOCUS       DASZOB010000143         6007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_123901, whole genome shotgun sequence.
ACCESSION   DASZOB010000143 DASZOB010000000
VERSION     DASZOB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123901"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VG709_02350"
     CDS             <1..1572
                     /locus_tag="VG709_02350"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWC31948.1"
                     /translation="PPPPAALAPYFTNLDGPVFALVNLPEVTKGALFARYSRSPKSLR
                     RLFLDEFADRALAAPPADEVGTQRAEDLYQRIFVEYGDDSVAQLGAAHLACEQSSQIL
                     AKVLEWGRLAAYLEQSTRYVPYDDRPGGSWRYLVPPEVERAGLAERFRGVADRAFETY
                     ARWLPRMQEFYADAYPKSADDSDFVYRMSIRAKACDALRGLLPAATRSNVGIFATGQA
                     YEQLLLRMRAHPLDEVRAYADMMLAELRKVIPAFLRRVDVPERGGEWSRYVATTRERT
                     REAAATVSVTAAEPRPDVTLTDFDAEGEIKVVAAALYPATGVPDDQLLEVARAMSPDE
                     RARVLAAYVGDRRNRRHRPGRAFERTSYRFDVLCDYGAFRDLQRHRMLTIEWQDLTID
                     HGYDMPRDVAEAGATRDWNRTMRESADLWSELRAAVIGDDAAQYAVAMAYRIRFAMQM
                     NAREAMHLIELRSSPQGHPTYRRIAHEMHRLIAEEAGHRVIADAMRFIDRSDVDLERL
                     EAERRSEARRRAAER"
     gene            1569..1727
                     /locus_tag="VG709_02355"
     CDS             1569..1727
                     /locus_tag="VG709_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31949.1"
                     /translation="MTKETAVVVALSLLGAAGLLASIVAAVLLWRDVARGDARAAEPA
                     TPDHEEEP"
     gene            1724..2398
                     /locus_tag="VG709_02360"
     CDS             1724..2398
                     /locus_tag="VG709_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31950.1"
                     /translation="MNDAPDVRDREAPTARRVVSPALFAVVVVCFFLPFVTIACNEQG
                     AGLAEGLEDLGATPQPGAAQELERTFTGWHLVTGNTDEDEAAAPPAEGLPGQQPTGRV
                     RDSQPIAIASLAVAVLGIALSWLALWIGPVAGATLGIAGVILLFVLKSNIEGIVPGGQ
                     FQLFVDFRWELGFWLAMVAFALAAAWSIYRLLSEARLRTRRAGAEPAPPPTADRDAIT
                     PPPPPE"
     gene            2401..2973
                     /locus_tag="VG709_02365"
     CDS             2401..2973
                     /locus_tag="VG709_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31951.1"
                     /translation="MRAAGPRKPLLRWLSPGMFGVVLISLALPFASVSCSSGEGDIEL
                     LTVRGFHLVTGSGPEVNPAVARAADDEALVAAADIGPQPFAALAALAAGAGLGLGFAR
                     PARARLVATVSAAVGTASVAIASLRLEVRLTNALAGQDAAAQGAVAVDRERGFWVALI
                     AFAVAGMTAGVAYAAQRPSIARPWRRSRGP"
     gene            complement(2970..4724)
                     /locus_tag="VG709_02370"
     CDS             complement(2970..4724)
                     /locus_tag="VG709_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWC31952.1"
                     /translation="MATRERPWLKSYPPDVPHSLEPYPERPVWSLLEDAARRFPDSTA
                     IAFPVAPMAKRLTYRQLVEEAERFAKALQALGVKKGDRVGLVLPNCPQFVVAFYAIQR
                     LGAVSVGNNPLYTQREMAHQLTDAGIDVLVVLDVLYPLIGAIREQVGLKKVVVTKVGD
                     YLGFPINKLSVIKQKKEAQKEGHPWPPVPSDADVLQWSDLMRNASGDLPPLQVNAKED
                     VACLVYTGGTTGLSKGAMLTHYNVVSNALQTSSWFVDVEEGNEGIMCVLPFFHSYGLN
                     AVMSVGIAKGMKLVLLPRFELEMALKAIAKEKPTLFPGVPRIYIAINESPETKNHDLS
                     SIRACFSGAAPLPVAVAERFESITGGRLVEGYGLTETSPVTHINPIYGKRKFGSIGLP
                     IPDTDCKIVDLDDPEKEMPPGEAGELCIAGPQIMKGYWNKPEETDEMIRVHEKDRIRW
                     LHSGDVATMDEEGYFSIVDRKKDMILVSGFNVYPTDVEGVLYRHPKIQKVAVVGIPDA
                     KTGEAVKAFVVLKEGESATEKEITDWAKDPEHGLTGYRAPKHIEFRDELPETMVGKVL
                     RRVLLDEEKQKQAAATGS"
     gene            4825..>6007
                     /locus_tag="VG709_02375"
     CDS             4825..>6007
                     /locus_tag="VG709_02375"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWC31953.1"
                     /translation="MANPMAAMLLEVARRGLPPVEGEVRGAGLERPVEILRDPWGVPH
                     VYAETLHDVWFAQGYVAASERLFQIEFFMRLGLGRLSELFSELSVPVDRYIRTVGWNR
                     IGARLAAGWDDTSREMSAAYTAGGRAWVETMPEKPMEYHVLDADPWLPTEGEAEVAGA
                     AAATFMAWSLSRNWDQELLRAEIAERLGIETMRALFPDLDTEAPIVEAGKDGAQSRLA
                     LLRDAILPPPGQGSNNWVVAGSRTASGKPLLANDPHLAVTWPSMWFECHLVAPGLDVA
                     GVALPFSPGIVIGHNDRIAWGFTNTEGDVQDLYLERLSDDGTAAEYMGRWEPLTIHRE
                     EIRVRGREEPEIVEIRESRHGPLMTAYTVGVGDPTIVEGGVRRAYALRWVGADAGVEP
                     ST"
BASE COUNT          828 a         2094 c         2152 g          933 t
ORIGIN      
        1 cccccccccc ccgcggccct cgcgccgtac ttcacgaacc tcgacgggcc ggtcttcgcg
       61 ctggtgaacc tgcccgaggt cacgaagggc gcgctgttcg cgcggtactc ccgctcgccg
      121 aagtcgctcc gccggctgtt cctcgacgag ttcgccgacc gcgcgctcgc cgcgcccccc
      181 gccgacgagg tcgggaccca gcgcgccgag gacctctacc agcggatctt cgtcgagtac
      241 ggcgacgact cggtggcgca gctgggcgcg gcgcacctcg cctgcgagca gtcgtcgcag
      301 atcctcgcga aggtcctgga gtggggccgt ctcgcggcgt acctggagca gtcgacaagg
      361 tacgtgccgt acgacgaccg gcccggcggc agctggcggt acctggtgcc gcccgaagtc
      421 gagcgggcgg ggctcgcaga gcggttcagg ggcgtcgccg accgcgcgtt cgagacctac
      481 gcgcgctggc tgccgaggat gcaggagttc tacgcggatg cctacccgaa gagcgccgac
      541 gacagcgact tcgtctaccg catgagcatc cgcgcgaagg catgcgacgc gttgcgcggg
      601 ctgctcccgg cggcgacgcg ctcgaacgtc gggatcttcg cgaccggaca ggcctacgag
      661 cagctgctgc tccgtatgcg cgctcatccg ctcgacgagg tacgcgcgta cgcggacatg
      721 atgctcgcgg agctccggaa ggtgatccct gcgttcctgc gccgcgtcga cgtcccagag
      781 cgcggcgggg agtggtcacg gtacgtcgcg acgacccgag agcggacgcg ggaggccgcg
      841 gccacggtct ccgtcacggc ggctgagccg cgcccggacg tgacgctcac cgacttcgac
      901 gcggaaggcg agatcaaggt cgtcgccgcg gccctgtacc cggcgacggg cgttcccgac
      961 gaccagctgc tcgaggtggc gcgcgctatg agccccgacg agcgggcacg cgtgctggcc
     1021 gcctacgtcg gcgaccggcg gaaccgccgt catcggccgg gccgcgcgtt cgagcggacg
     1081 tcgtaccggt tcgacgtgct gtgcgactac ggcgccttcc gtgacctgca gcgacaccgg
     1141 atgctcacga tcgaatggca ggacctgacg atcgaccacg ggtacgacat gccgcgcgac
     1201 gtcgccgagg ccggcgccac ccgcgactgg aaccggacga tgcgcgagtc ggcggacctc
     1261 tggagcgagc tccgcgccgc agtgatcggc gacgacgccg ctcagtacgc cgtcgcgatg
     1321 gcctaccgga tccgcttcgc gatgcagatg aacgcgcgtg aggcgatgca cctgatcgag
     1381 ctgcggagct ccccgcaggg ccatccgacg taccggcgga tcgcgcacga gatgcaccgg
     1441 ctgatcgccg aggaggcggg ccaccgcgtg atcgccgacg cgatgcgctt catcgaccgc
     1501 tccgacgtcg acctcgagcg ccttgaggcc gagcggaggt ccgaggcgag acgccgagcc
     1561 gcggagcggt gacgaaggag acggcggtcg tcgtggcgct gtcgttgctc ggcgcagccg
     1621 ggttgcttgc ctcgatcgtc gcggcggtac tactgtggcg cgacgtcgca cgtggcgatg
     1681 cccgcgcggc cgaacccgcg accccggacc acgaggagga gccatgaacg acgcgccgga
     1741 cgtccgcgac cgagaagcgc cgacagctcg aagggtcgta tcgccggcgc tcttcgcggt
     1801 cgtcgtcgtc tgcttcttcc tgccgttcgt cacgatcgcg tgcaacgagc agggtgcggg
     1861 cctcgcggag gggctcgagg acctaggcgc gaccccgcag cccggcgccg cgcaggagct
     1921 cgagcgcacg ttcacggggt ggcacctagt caccggcaac accgacgaag acgaggccgc
     1981 cgctcccccg gccgagggcc tgccggggca gcagccgacg ggccgggtcc gtgactctca
     2041 gccgatcgcg atcgcgtcgc tggcggtcgc ggtgctgggg atcgcgctgt cgtggctcgc
     2101 gctgtggatc gggccggtcg ctggggcgac gctcgggatc gcgggggtca tcctgctgtt
     2161 cgtgctgaag agcaacatcg agggcatcgt cccgggcggg cagttccagc tcttcgtcga
     2221 cttccggtgg gagctcgggt tctggctcgc gatggtggcc ttcgcgctcg cggcggcgtg
     2281 gagcatctac cggctgctgt cggaggctcg gctcaggacg cgccgggccg gcgccgagcc
     2341 ggcgccgcca ccgaccgcgg accgcgacgc catcacgccg ccaccgcccc cggaataggg
     2401 atgcgcgcgg ctgggccgcg gaagccgctg ctgcgctggc tctcccccgg gatgttcggg
     2461 gtggtgctga tctcgctcgc gctgccgttc gcctccgtct cgtgcagctc gggagaaggc
     2521 gacatcgagc tcctcacggt gcggggattc cacctcgtca ccgggagcgg cccggaggtg
     2581 aaccccgctg tcgcgcgtgc ggccgacgat gaggcgctcg tcgcggcggc cgacatcggg
     2641 ccgcagccgt tcgcggccct cgcggccctc gcggccggcg cgggactggg gctgggcttc
     2701 gcgcgcccgg cccgcgcgcg gctcgttgcg accgtgagcg cggcggtcgg cacggcgtcg
     2761 gtcgcgatcg cgtcgttgcg gctcgaggtc cggctcacga acgcgctcgc cggccaggac
     2821 gccgcggcgc agggcgccgt cgcggtcgac cgagagcgcg gcttctgggt ggcgctgatc
     2881 gcgttcgccg tggccgggat gacggccggc gtcgcgtacg cggcccagcg cccctcgatc
     2941 gcccggccct ggcgtcggtc gcgcgggccc tagcttcccg tcgcggccgc ctgcttctgc
     3001 ttctcctcgt cgaggaggac ccggcgcagg accttgccga ccatcgtctc gggaagctcg
     3061 tcgcggaact cgatgtgctt gggcgcccgg taccccgtca ggccgtgctc cgggtccttc
     3121 gcccagtccg tgatctcctt ctcggtcgcg ctctccccct ccttcagcac gacgaacgcc
     3181 ttcacggcct cgccggtctt cgcgtcgggg atgccgacaa cggcgacctt ctggatcttc
     3241 gggtgccggt agaggacgcc ctcgacgtcg gtcgggtaca cgttgaaccc cgacacgagg
     3301 atcatgtcct tcttccggtc gacgatcgag aagtacccct cctcgtccat cgtcgccacg
     3361 tcgccgctgt gcagccaacg gatgcggtcc ttctcgtgga cgcggatcat ctcgtcggtc
     3421 tcctcgggct tgttccagta gcccttcatg atctgcgggc cggcgatgca gagctctcct
     3481 gcctcgcccg gcggcatctc cttctcgggg tcgtcgaggt cgacgatctt gcagtcggtg
     3541 tcggggatcg gcagcccgat cgagccgaac ttccgcttcc cgtagatcgg gttgatgtgc
     3601 gtgaccggcg acgtctcggt gagcccgtac ccctcgacga ggcgaccacc ggttatcgac
     3661 tcgaacctct cggcgacggc aacgggtagc ggcgcggcgc ccgagaagca ggcgcggatc
     3721 gacgacaggt cgtggttctt cgtctcgggc gactcgttga tcgcgatgta gatccgcgga
     3781 acgccgggga agagcgtcgg cttctccttc gcgatcgcct tgagcgccat ctcgagctcg
     3841 aatcgcggaa gcagcacgag cttcatcccc ttggcgatcc cgacgctcat gaccgcgttc
     3901 agaccgtagg agtggaagaa gggcaggacg cacatgatgc cctcgttgcc ctcctcgacg
     3961 tcgacgaacc aggacgaggt ctggagggcg ttgctgacga cgttgtagtg ggtgagcatc
     4021 gcgcccttcg agagcccggt cgtgcctccc gtgtacacga gacacgcgac gtcctccttc
     4081 gcgttcacct ggagcggcgg gaggtcgccg gaggcattcc gcatgaggtc ggaccactgc
     4141 agcacgtcgg cgtcggaggg gaccggcggc caggggtggc cttccttctg cgcctccttc
     4201 ttctgcttga tgaccgagag cttgttgatc gggaaaccga ggtagtcgcc gaccttcgtg
     4261 acgacgacct tcttgagccc gacctgctcc cggatcgcgc cgatcagcgg gtacagcacg
     4321 tcgagcacga cgaggacgtc gatgcccgcg tccgtcagct ggtgggccat ctcccgctgc
     4381 gtgtacagcg ggttgttgcc gaccgagacc gctccgagcc tctggatcgc gtagaacgcg
     4441 accacgaact gagggcagtt gggcagcacg aggccgaccc ggtcgccctt cttgacgccg
     4501 agcgcttgca gcgccttcgc gaagcgctcg gcctcctcga cgagctgccg gtacgtgagg
     4561 cgcttcgcca tcggggccac cgggaacgcg atcgcggtgc tatcagggaa cctgcgcgcg
     4621 gcatcctcga gcagcgacca gaccgggcgc tcgggatacg gctcgagcga gtgcgggacg
     4681 tccggagggt agctcttcag ccaggggcgc tcacgcgtcg ccatgtcgct cctttccgtg
     4741 ccgaccagtc gattatgtcc gacgctcgaa cgggcggaca aaccggcttg ccctccttgg
     4801 cgctgctccg tagcgtgagc gcccatggcg aacccgatgg ccgcgatgct gctcgaggtc
     4861 gcgcggcgag ggctgccacc ggtcgagggc gaggtccggg gcgccggcct cgaacggccg
     4921 gtcgagatcc tccgcgaccc ctggggcgtt ccgcacgtct acgccgagac gctgcacgac
     4981 gtgtggttcg cgcaggggta cgtcgccgcg tcagagcgcc tgttccagat cgagttcttc
     5041 atgcgtctcg ggctcgggcg cctctcagag ctcttcagcg agctcagtgt tccagtcgac
     5101 cggtacatcc gcaccgtggg ctggaaccgc atcggcgctc ggctcgcggc cggctgggac
     5161 gacacgtcga gggagatgag cgccgcctac acggcgggag gtcgcgcctg ggtcgagacg
     5221 atgccggaga agcccatgga gtaccacgtg ctcgacgcgg atccgtggct gccgaccgag
     5281 ggcgaggcgg aggtcgccgg tgccgcggcc gccacgttca tggcctggtc gctctcgcgg
     5341 aactgggacc aggagctgct ccgggccgag atcgccgagc ggctcggcat cgagacgatg
     5401 cgcgcgctgt tccccgatct cgacaccgag gccccgatcg tcgaggccgg caaggacggc
     5461 gcgcagagcc gcctcgcgct gctccgcgac gcgatccttc cgccgccggg gcaggggtcc
     5521 aacaattggg tcgtggcggg atcccggacg gcatccggga agccgctgct cgcgaacgat
     5581 ccgcacctcg cggtgacgtg gccgtcgatg tggttcgagt gccacctcgt cgcgccgggg
     5641 ctcgacgtgg caggcgtcgc gctgccgttc tcgccgggga tcgtgatcgg tcacaacgac
     5701 cgaatcgcgt gggggttcac gaacaccgag ggcgacgtcc aggacctgta cctcgagcgc
     5761 ctctccgacg acggcaccgc cgccgagtac atgggtcgct gggagccact gacgatccac
     5821 cgcgaggaga tccgcgttcg cggtcgcgag gagccggaga tcgtcgagat ccgcgagtcc
     5881 cgccacgggc cgctgatgac cgcctacacg gtcggcgtcg gcgacccgac gatcgtggag
     5941 ggaggggtcc gtcgcgcgta cgcgctgcga tgggtcggcg ccgacgccgg cgtcgagccg
     6001 tcgacgc
//
LOCUS       DASZOB010000144         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_53611, whole genome shotgun sequence.
ACCESSION   DASZOB010000144 DASZOB010000000
VERSION     DASZOB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53611"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1400)
                     /locus_tag="VG709_02380"
     CDS             complement(186..1400)
                     /locus_tag="VG709_02380"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC31954.1"
                     /translation="MDVEIRKLERERVAEAVRMLFVAFGDLPRQREVDLEERVLGEGT
                     WYGALDGEAIVGSAAHIPLRMTVPGSELTLGAVTSVGVLPTHRRRGVLTALMRRQLDD
                     LRGAELAVAGLWASEAGIYLRFGYGHAIPAASFSIERPYNAFSSDVPTSGRVRLIERD
                     DAIRQLAPIYDRVRLERPGFLERDEKWWEYLLEHHHDEKQQAAYFALHEGADGPDGYA
                     RYRIERKWAIEGPRSVLEVDEVVAATDDAYAALWRYVLDVDLVHTVRGENHPTDEPLF
                     HMLREPRRLGFSLRDGLWLRLVDVPTALASRRYRAGGRLVVEVRDGFCPGNDGRFELE
                     GDRDGATCRETDRDPDLVCDVRDLGAAFMGGVPVTTLVRAGRVVEETDGAALRTDAMF
                     AWDRTPWCSHVF"
     gene            complement(1410..2051)
                     /locus_tag="VG709_02385"
     CDS             complement(1410..2051)
                     /locus_tag="VG709_02385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWC31955.1"
                     /translation="MTQRDAELAGSLAAAYTAFAATFRGPRPAFWNRMTLTGLSLGTL
                     ALALEPRARQLRVRGRDVATGLASAAALYGIFRIGDRMARRIMPSGASDIDSVYDLRT
                     LGDRAAIAARLAAVIGPAEEIFWRGFVQRRLADRFETYQGAALAAAAYGGAHVVTLNP
                     TLVGAATVAGAFWSGLSAAGASLGALIVSHAAWDVLIFLVAPTTPKDPRTVAP"
     gene            complement(2048..>2541)
                     /locus_tag="VG709_02390"
     CDS             complement(2048..>2541)
                     /locus_tag="VG709_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HWC31956.1"
                     /translation="YKYRALGVPLVFLLMGPLMVIGSYYVTSGTFDPRAIAVSIPVGF
                     LVAAILHGNEWRDISEDARAGIATLSTRLGRRVAHISYVSLVLGAYLALAAAVFFGAL
                     PTSALLAMLSLPLLVRAIRASELGASGQQRAIAMIDLETAQLHAAFGFLLVAGIALAR
                     LVA"
BASE COUNT          410 a          908 c          884 g          339 t
ORIGIN      
        1 ggccggttgt gtgccggccc cttcacttat gagcgagttc cggagcgcac ggcccgatgt
       61 cacaggcgtg catgagcgcc actaggcctt tggcgttcgt gcaggtcagg cgtccacacg
      121 agtgacggcg tcacggtccg ccatgtgcgc gcgtgggttc aaatcctgcc gcgcccacgg
      181 acgcttcaga agacgtgcga gcaccagggc gtccggtccc acgcgaacat cgcgtccgtg
      241 cgaagcgccg cgccgtcggt ctcctcgacc accctgccgg cgcgcacgag cgtcgtgacc
      301 gggactccgc ccatgaacgc cgctccgagg tctcgaacgt cgcacacgag atcaggatcg
      361 cggtcggtct cccggcacgt cgcaccgtcg cgatcgccct cgagctcgaa ccgtccgtcg
      421 ttccccgggc agaagccgtc cctgacctcg acgacgagac gtccgccggc gcggtagcgg
      481 cgcgaggcga gcgccgtggg cacgtcgacg agccggagcc ataggccgtc acggagcgag
      541 aagccgagcc ggcgtggctc gcgcagcatg tggaacagcg gctcgtcggt cgggtggttc
      601 tcgccgcgga ccgtgtgcac gaggtcgacg tcgagcacgt accgccaaag cgcggcgtac
      661 gcgtcgtcgg tcgccgcgac gacttcgtcg acctcgagga cgctgcgggg cccttcgatc
      721 gcccacttcc gttcgatccg gtaccgcgcg tatccgtccg gtccgtccgc gccctcgtgc
      781 agcgcgaagt acgcggcctg ctgcttctcg tcgtgatggt gctcgagcag gtactcccac
      841 cacttctcgt cacgctcgag gaatcccgga cgctcgagac gaacgcgatc gtagatcggc
      901 gcgagctgac gaatcgcgtc gtcccgctcg atcagccgca cgcgtcccga cgtcggaacg
      961 tccgaggaga acgcgttgta cgggcgctcg atcgagaagc tcgcggcggg gatcgcgtgg
     1021 ccgtacccaa accgcaggta gatgcccgcc tcggacgccc acagcccggc cacggcgagt
     1081 tcggcaccgc ggagatcgtc gagctgccgc cgcatcaacg cggtcaggac gccacgcctg
     1141 cggtgtgtcg gaaggacacc gacgctcgtg acggcgccga gcgtgagctc cgaccctggg
     1201 acggtcatcc gcagcgggat gtgcgccgcc gagccgacga tcgcctctcc gtcgagcgcc
     1261 ccgtaccacg ttccctcgcc gaggacgcgc tcctcgaggt ccacttcgcg ctgacgcggg
     1321 aggtcgccga aggcgacgaa cagcatcctg accgcctcgg cgacgcgctc acgctcgagc
     1381 ttccggatct cgacgtccac gccgcgagtc tacggcgcta ccgtgcgcgg atctttcggg
     1441 gtcgttgggg cgacgaggaa gatcagcacg tcccacgccg cgtggctcac gatgagggcg
     1501 ccgagcgacg cccccgcggc cgagagaccg gaccagaacg cccccgcgac ggtcgccgca
     1561 ccgacgagcg tcgggttcag cgtcacgacg tgcgcgccgc cgtacgcggc cgctgcgagc
     1621 gccgcccctt ggtacgtctc gaaccggtcc gcgagccgcc gctggacgaa ccctcgccag
     1681 aagatctcct cggccggccc gatgacggcc gcgagacggg ctgcgatcgc ggcacgatcg
     1741 ccgagcgttc gcaggtcgta gacggagtcg atgtccgaag cgcccgacgg catgatgcgg
     1801 cgggccatgc ggtcgccgat ccggaagatc ccgtaaaggg ccgcggccga tgcgagcccg
     1861 gttgcaacgt cgcgaccccg cacccggagc tgtcgggcgc gaggctcgag cgccagggcg
     1921 agcgtcccga gcgagagacc cgtgagcgtc atgcggttcc agaacgccgg cctcgggccc
     1981 cggaacgtcg ccgcgaaggc ggtgtaggcc gccgcgaggg agcccgcgag ctcggcgtcg
     2041 cgctgcgtca cgccacgagc cgcgcgagcg cgatgcccgc gaccagcagg aagccgaatg
     2101 cggcgtggag ctgcgccgtc tcgaggtcga tcatcgcgat cgcgcgctgc tgtccgctgg
     2161 cgccgagctc ggacgcgcgg atcgcgcgga cgagcaacgg gagcgacagc atcgcgagca
     2221 gcgccgaggt cggaagcgcg ccgaagaaga ccgccgcagc gagcgcgaga tacgccccga
     2281 gcacgagcga cacgtagctg atatgtgcga cccggcggcc gagacgtgtc gacaacgtcg
     2341 cgatcccggc cctcgcgtcc tcgctgatat ctcgccactc gttcccgtga aggatcgcgg
     2401 cgacgaggaa cccgacgggg atgctcaccg cgatggcgcg cggatcgaac gtgccgctcg
     2461 tcacgtaata cgagccgatg accatgagcg gccccatcaa caggaagaca agcgggacgc
     2521 cgagcgcgcg gtacttgtac t
//
LOCUS       DASZOB010000145         5877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_123999, whole genome shotgun sequence.
ACCESSION   DASZOB010000145 DASZOB010000000
VERSION     DASZOB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123999"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VG709_02395"
     CDS             complement(<1..326)
                     /locus_tag="VG709_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31957.1"
                     /translation="MSPARQLLDRVREDRRVRRDFERLILDSGLEGLDREPQPRPQRA
                     RAKRPRTPTWRVSRAALAILGVLVLVSSVVVMQPFPYDLPAPYQTAFVYARDGRPVAT
                     LRPPEDR"
     gene            389..1195
                     /locus_tag="VG709_02400"
     CDS             389..1195
                     /locus_tag="VG709_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWC31958.1"
                     /translation="MDAQPRLIFSTAPFFRHPLRDAFRHIRDAGYDAVEVMVTSDPAT
                     QDGRTLRELAAEHELSIEALHAPFLLLTRRVWTTDPVTKIYRATQVAEQAGIPLVVVH
                     PPYRWQVRYKRWVDQSLAEFAARVGVAIAVENMFPVRLRGDRGLRFHVSQEFEDLDRF
                     PHLVLDTSHLAVSGIDILEAYERYRTKVVHFHLSNNAGKGWDSHLPVDDDKGVLPLDR
                     LLDAVSGDGFAGTVSLELDLRPHFDDEEALHDLLVANREFCEARLTARTP"
     gene            1195..1326
                     /locus_tag="VG709_02405"
     CDS             1195..1326
                     /locus_tag="VG709_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31959.1"
                     /translation="MRRWTLITLVVLTVTLALVAFFQIRAAQRPERYPGPGVSPTAP"
     gene            complement(1323..2297)
                     /locus_tag="VG709_02410"
     CDS             complement(1323..2297)
                     /locus_tag="VG709_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWC31960.1"
                     /translation="MAQVIAIADADRCRAITAAGRRCRNRGVDASGYCRVHRSEPADD
                     VRPEPDQLQRALEFVRRRLTGDYDIDEFGFDAQLTEEVLAPLARPLHERWWRVDWIGM
                     ENVPSEGAALLVSNHAGTVPWDAICVKFGLLDRHPARRHLRLLAADLAFRMPVVGPLA
                     RKSGNTLACEEDALRLLESGELLGVFPEGYKGVGKPYRERYKLQRFGRGGFIELALRA
                     KAPIVPVSIVGSEEIAPMLWNAKPRARIFGFPYFPITPTVPWLGPLGAIPLPSKWIVE
                     FGEPIRTDEFPDDAWQDGLTVFELTDQVRDIVQRTLLRNLRLRRSVWY"
     gene            complement(2297..3349)
                     /locus_tag="VG709_02415"
     CDS             complement(2297..3349)
                     /locus_tag="VG709_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC31961.1"
                     /translation="MGQRILITGISGHLAARLAHRLEQDPEVEYVVGVDLEEPRTDLE
                     RTEFVRADIRNPLVVKVLQTTEVDTVVHLSILATPSGAGGRSRMKEINIIGTMQLLAA
                     CQRAERLRKIVMKSTTAVYGAHPRDPAVFTEDMSAQSEPRGGYTKDAIEVESYARAFA
                     RRRIDVQLTMLRFANFIGPDIETPFTRYFSLPVVPTALGYDPRLQFLHEDDAAEVLYR
                     AVREDHRGVYNVAGDGVVLLSQALRLIGKPSLPVVLPLVQPIAAGVRRFGLVDFATDQ
                     LRFLLYGRVADTARLRHEFGYTPRFTTKEALLDFARSRPLRRIVSNERISDWERDLYA
                     FLRRKGQERFERTRRG"
     gene            complement(3362..4162)
                     /locus_tag="VG709_02420"
     CDS             complement(3362..4162)
                     /locus_tag="VG709_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWC31962.1"
                     /translation="MRPLVFLSDYGLDDEFVGVCHAVVARIAPGAAVIDLTHGIPPHD
                     VLRGALVLAEASRHAPENAVYLAVVDPGVGTERRGVALEAGMSLLVGPDNGLLWPAAE
                     ALGGVRAAVEIPLPPGASATFHGRDVFAPAAARLSGGEGLTDVGAAVDPSTLARLEPP
                     SPEVRSGMLRCAVLAVDRFGNVQLAARVEHLERAELDRAPQVELRSGLRAAPVRRART
                     FAEIPEGEHAILVDSAGWLAVVRNRASAAEALRLAPGDDVVLAAPGFG"
     gene            complement(4162..4845)
                     /locus_tag="VG709_02425"
     CDS             complement(4162..4845)
                     /locus_tag="VG709_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWC31963.1"
                     /translation="MRRAAAVAACVAVVLSSGCGGGGDVAARTVRFSTDDGVELEGDV
                     RGGGTTGVALAHMYPTDRTSWEPFAERLAAEGYLTLAFDFRGYGGSEGERTIPEIWRD
                     VVAAAGELRSHGAERVVLVGASMGGTASLVAASRSAVDGVVTISAPSTFEGLTASPDV
                     VRAVQAPKLFVAASADGAAAAAAQSFYNDSPQPKRVEIVGGDEHGTELLEGSHGEVVR
                     NVILDFLRV"
     gene            complement(4850..4948)
                     /locus_tag="VG709_02430"
     CDS             complement(4850..4948)
                     /locus_tag="VG709_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HWC31964.1"
                     /translation="MPSLIKKRRKKMRKKKHKKLLKRTRWQRRQRT"
     gene            complement(4965..>5877)
                     /locus_tag="VG709_02435"
     CDS             complement(4965..>5877)
                     /locus_tag="VG709_02435"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HWC31965.1"
                     /translation="VGPFGGAMDQYDEVLQRALDARGVTVLLGGLDTGKTTFARRIAS
                     AGVDAGLRVAVVDADCGQSTIGPPGTVGLRLIRSHADLEPESLAKPDALAFVGSTSPQ
                     GHLLQLVAGTRVILDRARAEGPDVVVVDTTGLVSGVYGQVLKYNKLAVVAPDIVVGLS
                     RGEELEPLLGVARRFFAAEIVSLRVHPDVAPTSVEQRATNREEAMRRYFSGQLQRWRV
                     KSTVFMPSLPTLFDLSALDRILAGLSDGKGTCIGVGHLEYVEGEGALRLISPVPEAPR
                     ALILGSVRLDEAFRARRVDLRNLFGSD"
BASE COUNT          870 a         2123 c         2058 g          826 t
ORIGIN      
        1 cgatcctcgg gtgggcggag cgtcgcgacg ggccggccgt cgcgcgcgta cacgaacgcc
       61 gtctggtacg gcgccggaag gtcgtacggg aacggctgca tcacgacgac cgaggagacg
      121 agcacgagca ccccgaggat cgcgagcgcc gcgcgcgaca cccgccacgt cggggtgcgt
      181 ggcctcttcg cccgcgctcg ctgcggcctc ggctgcggct ccctgtcgag accctcgagc
      241 ccgctgtcga ggatcagtcg ctcgaagtcg cgacggacgc ggcggtcctc gcgcacgcgg
      301 tcgagcagct ggcgggccgg gctcacggac ctctattttc cccgcgatcc cgatgtgatt
      361 gcggcgcgtg tctagaatgg cccgaccgtt ggacgcgcaa ccacgcctga tcttctcgac
      421 ggcaccgttc ttccgacatc cgctgagaga cgcgttccgg cacatccgcg acgcgggcta
      481 cgacgcggtc gaggtcatgg tgacctcgga tcccgcgacg caggacggcc ggacgctccg
      541 agagctggcg gcggagcacg agctgtcgat cgaggccctg cacgcgccgt tcctcctgct
      601 cacccggcgc gtgtggacga ccgaccccgt cacgaagatc taccgcgcca cacaggtcgc
      661 cgagcaggcc ggcatcccgc tggtcgtcgt gcacccgccg taccggtggc aggttcggta
      721 caagcggtgg gtcgatcaga gcctcgcgga gttcgcggcg cgcgtcggcg tcgcgatcgc
      781 cgtcgagaac atgttcccgg tgaggctgcg cggagaccgc gggctgcggt tccatgtcag
      841 ccaggagttc gaggacctcg accgcttccc gcatctcgtc ctcgacacca gccacctcgc
      901 cgtgtctggg atcgacatcc tcgaggcgta cgagcggtac cggacgaaag tcgttcactt
      961 ccacctctcg aacaacgcgg gcaagggatg ggactcccac ctccccgtcg acgacgacaa
     1021 gggcgtcctt ccgctcgacc ggctgctcga cgccgtctcc ggggacgggt tcgccgggac
     1081 cgtctcgctc gagctcgacc tcaggccgca cttcgacgac gaggaggccc tccacgacct
     1141 cctcgtggcg aaccgcgagt tctgcgaggc gcgcctcacc gcccggacgc cctagtgcgt
     1201 cgctggacgc tgatcacgct cgtcgtgctc acggtgacgc tcgcgctcgt cgccttcttc
     1261 cagatccgcg ccgcgcagcg cccggagcgc tacccgggcc cgggggtgtc gccgacggcg
     1321 ccctagtacc agaccgagcg ccggagccgg aggttccgca gcagcgtccg ctggacgatg
     1381 tcgcggacct ggtcggtgag ctcgaacacc gtgagcccgt cttgccaggc gtcgtccggg
     1441 aactcgtcgg tgcggatcgg ctcgccgaac tcgacgatcc atttcgacgg cagcgggatc
     1501 gctccgagcg ggccgagcca ggggaccgtc ggagtgatcg ggaagtaggg gaacccgaag
     1561 attcgggccc gcggcttcgc gttccacaac atcggcgcga tctcctcgga gccgacgatc
     1621 gacaccggca cgatcggggc cttggcgcgg agcgcgagct cgatgaagcc gccgcggccg
     1681 aaccgctgga gcttgtagcg ctcgcggtac ggctttccga cgcccttgta cccctcgggg
     1741 aacacgccga ggagctcgcc gctttcgagc agccgcaggg cgtcctcctc gcacgcgagc
     1801 gtgttgcccg acttgcgcgc gagcggtccg accaccggca tgcggaacgc gaggtcggcg
     1861 gcgaggagcc tgaggtgccg gcgagccggg tggcggtcga ggagcccgaa cttcacgcag
     1921 atcgcgtccc atggaacggt tccggcgtgg ttcgacacga gcagcgcggc gccttccgac
     1981 gggacgttct ccatcccgat ccagtcgacc cgccaccatc gctcgtggag cggccgcgcg
     2041 agcggcgcca agacctcctc cgtgagctgc gcgtcgaagc cgaactcgtc gatgtcgtag
     2101 tcgccggtga ggcggcgccg cacgaactcg agcgcgcgct ggagctggtc gggctcgggc
     2161 cggacgtcgt ccgcgggctc gctgcggtga acccggcagt accccgatgc atcgacgccg
     2221 cggttgcggc agcgccggcc ggcggccgtg atggccctgc atctgtcggc gtcggcgatc
     2281 gcgatgacct gcgccatcag cctctcctcg tccgctcgaa gcgctcctga cccttccgcc
     2341 ggaggaacgc gtacaggtct cgctcccagt cgctgatccg ctcgttcgag acgatccgcc
     2401 gcagcggcct cgacctcgcg aaatcgagga gcgcctcctt ggtcgtgaac cggggcgtgt
     2461 acccgaactc gtgccgcagc ctcgcggtgt cggctacccg gccgtagagc aggaaccgca
     2521 gctgatcggt cgcgaagtcg accagcccga accgccgaac gcccgcggcg atcggctgca
     2581 cgagcggcag cacgaccggg agcgacggct tgccgatcag ccggagcgcc tgagagagca
     2641 gcacgacgcc gtcgccggcg acgttgtaga cgccacggtg gtcttcgcgg accgcccggt
     2701 acagcacctc ggccgcgtcg tcctcgtgga ggaactggag cctcgggtcg tacccgagcg
     2761 ccgtcggaac gaccggcaac gagaagtacc gcgtgaaggg cgtctcgatg tcgggaccga
     2821 tgaagttcgc gaagcggagc atcgtgagct gcacgtcgat ccgcctgcgc gcgaacgcgc
     2881 gcgcgtagct ctcgacctcg atcgcgtcct tcgtgtagcc gccgcgtggc tccgactgcg
     2941 ccgacatgtc ctcggtgaac accgcggggt cgcgcgggtg tgcgccgtag acggccgtcg
     3001 tcgacttcat gacgatcttg cgaagtcgct ccgcccgctg gcacgcggcg agcagctgca
     3061 tcgtcccgat gatgttgatc tccttcatcc tcgagcggcc gccggctccc gacggcgtcg
     3121 cgaggatgct gaggtgcacg accgtgtcga cttcggtcgt ctgcagcacc ttcacgacga
     3181 gcgggttgcg gatgtcggct cgcacgaact ccgtccgctc gaggtcggtc cgcggctcct
     3241 cgaggtccac cccgacgacg tactcgacct ctgggtcctg ctcgaggcgg tgcgcgagcc
     3301 tcgcggcgag gtgtccggat atcccggtga tgaggattcg ctggcccatc ggcacgtgag
     3361 gctacccgaa tcccggcgcg gcgagcacga cgtcgtcgcc cggcgcgagg cgcagcgcct
     3421 cggcagcgct cgcccggttg cgcaccactg cgagccaccc ggcagagtcc acgaggatcg
     3481 cgtgctctcc ctccgggatc tctgcgaacg tccgcgcccg tcgtaccggc gcggcacgga
     3541 gccccgagcg cagctcgacc tgcggcgcgc ggtcgagctc ggcacgctcg aggtgctcga
     3601 cgcgtgcggc gagctgcacg ttcccgaacc ggtcgaccgc gagcacggcg caacggagca
     3661 tgccgctccg cacctccggg gacggcggct cgaggcgcgc gagcgtcgat ggatcgaccg
     3721 cggcgccgac gtcggtaagg ccctcacctc cggagagccg agcggcggcg ggcgcgaaca
     3781 cgtcgcggcc gtggaacgtc gccgacgcgc cgggcggaag cgggatctcg acggccgccc
     3841 gaacgccgcc gagcgcctcc gccgccggcc acagcagtcc gttgtcggga ccaacgagga
     3901 gggacatccc agcctcgagg gccacgccgc ggcgttccgt gccgacgccg ggatcgacga
     3961 ccgccaggta gacggcgttc tcgggggcgt gccgcgacgc ctcggccagg acgagcgcgc
     4021 cgcggagcac gtcgtgcggc gggatcccat gcgtgaggtc gatcaccgcc gcgcccggcg
     4081 cgatgcgcgc cacgactgcg tggcacacac cgacgaactc gtcgtcgagc ccgtagtcgg
     4141 agaggaagac gagcgggcgc atcagacgcg caggaagtcg aggatcacgt ttcgcacgac
     4201 ctctccgtgt gaaccctcga ggagctcggt cccgtgctcg tcgccgccca cgatctcgac
     4261 gcgcttgggc tgcggcgagt cgttgtagaa cgactgcgcg gcggccgctg ccgcgccgtc
     4321 cgcgctcgcc gcgacgaaca gcttcggcgc ctggaccgcg cggacgacgt ccggtgaggc
     4381 cgtgagcccc tcgaacgtcg acggggcgga gatcgtcacg acgccgtcga ccgccgagcg
     4441 cgacgcggcg acgagcgacg cggtgcctcc catgctcgcg ccgaccagca cgacccgttc
     4501 ggccccgtgc gagcgcagct ctcccgcggc cgcgacgacg tcgcgccaga tctccgggat
     4561 cgtccgctcc ccctcggaac cgccgtaccc gcggaaatcg aaagccagcg tgaggtatcc
     4621 ctcggcggca aggcgctccg cgaacggctc ccacgacgtg cgatccgtcg ggtacatgtg
     4681 ggcgagcgcc acgccggtgg tccctccgcc gcggacgtcg ccctcgagct cgacgccgtc
     4741 atcagtcgag aagcgcacgg tccgggcggc gacgtcgccg ccaccgccgc agcccgacga
     4801 cagcacgacg gcgacgcatg cggcaacggc tgccgcccga cgcacggcgc tacgtgcgct
     4861 gacggcgctg ccagcgcgtg cgcttcagaa gcttcttgtg cttcttcttc cgcatcttct
     4921 tgcggcgctt cttgatgagc gacggcatgc gttccttccg ttcgtcagtc ggagccgaag
     4981 aggttccgaa ggtcgacgcg cctcgcgcgg aaagcctcgt cgaggcgcac cgacccaagg
     5041 ataagggccc gtggggcctc cggcacgggg gagatgaggc ggagggcccc ctcgccctcg
     5101 acgtactcga ggtggccgac gccgatgcac gttcccttcc cgtccgagag cccggccagg
     5161 atacggtcga gcgcggagag atcgaacagc gtggggagcg acggcatgaa caccgtcgac
     5221 ttcacgcgcc agcgctggag ctgtcccgag aagtaccggc gcatcgcctc ctcacggttt
     5281 gtcgcgcgct gctcgaccga tgtcggcgcc acgtcgggat ggacgcggag cgagacgatc
     5341 tccgccgcga agaacctgcg cgccacgccg agcagcggct cgagctcctc gccgcgcgag
     5401 aggccgacga cgatgtccgg cgccacgacc gcgagcttgt tgtacttcag gacctgcccg
     5461 tagacgccgg agacgagccc ggtcgtgtcg acgacgacga cgtcggggcc ctcggcccgc
     5521 gcgcggtcga ggatcacgcg ggtcccggcg acgagctgga gcaggtggcc ctgcggcgac
     5581 gtcgacccca cgaacgcgag cgcgtcgggc ttcgcgagcg actccggctc gaggtcggcg
     5641 tgcgaccgga tgaggcgcag gccgacggtg ccaggaggtc cgatcgtcga ctgcccgcag
     5701 tcggcgtcga cgacggcgac gcgcaggccg gcatccacgc cggccgaggc gatccgcctg
     5761 gcgaacgtcg tcttcccggt gtcgaggccg ccgagcagga cggtcacgcc gcgagcgtcg
     5821 agcgcccgct gcagcacctc gtcgtactgg tccattgctc ctccgaaggg ccccacc
//
LOCUS       DASZOB010000146         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_124023, whole genome shotgun sequence.
ACCESSION   DASZOB010000146 DASZOB010000000
VERSION     DASZOB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124023"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VG709_02440"
     CDS             <1..748
                     /locus_tag="VG709_02440"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWC31966.1"
                     /translation="FLNELGPVLRAIDPEEANAFIHAMNQALTGNEATVRLLLDSGAH
                     LATELGSIDRQIKSLIGSSDEALGIYARQSDDLGRLIDDLDTVGGKLAGMTGAINSLL
                     VNFAVVQEELDELLSENRGNIDATLSGLLDITRLLDDNRGALERTLCTLPAGVVSYDQ
                     TSSWGEWFNVRIVEFVVKDENSREIASSGELQQQRGNRDPRGVAVCPRGESVRKQLRE
                     GHGRLGGLEVQPEEARLPEWLDTITGASRG"
     gene            741..>1782
                     /locus_tag="VG709_02445"
     CDS             741..>1782
                     /locus_tag="VG709_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWC31967.1"
                     /translation="MARGRLSLLERNQRVIGTLALLVILGGTAFALLLQGGFLTQKYR
                     VTALFTDAAGVREGDNLTVAGLVAGRVEAVRVEGGQVAMELGVQEDVGLPADSRAEVV
                     VETLLGRKSVALLPGDSEEPLEDGDVIPLERTTTPVDIPEFNDISVELLEASDAEALE
                     TFLEDVSEITKGKAQEVQALIRGLEQVTAAVDARRDELEGLIDSLRVVSTTLGQRDQT
                     IVSLIDNLDVVLGNLAERQDALERLLVATAGATHETADLVRRNRLVLDDTLRFLHRDL
                     RVLDRHQIDIAAAISYLENGVQGYQGVGYSQCTRPGVGCVPNAWANIFIQSLGPLGVD
                     AIVGECGLVDKLF"
BASE COUNT          275 a          621 c          633 g          253 t
ORIGIN      
        1 gttcctgaac gagctggggc cggtgctccg cgcgatcgac ccagaggagg ccaacgcgtt
       61 catccacgcg atgaaccagg cgcttaccgg gaacgaggcg acggtacggc tcctgctcga
      121 ctcgggcgcg cacctggcga cagagctcgg gagcatcgac cgccagatca agagcctgat
      181 cggctcctcc gacgaggcgc tgggcatcta cgcgcgccag tcggacgatc tcgggcggct
      241 gatcgacgac ctcgacaccg tcggcggcaa gctcgccggg atgaccggcg ccatcaacag
      301 cctgctcgtg aacttcgcgg tggtccagga ggagctcgac gagctgcttt cggagaaccg
      361 cggcaacatc gacgcgacgc tctccggcct cctcgacatc acccgtctcc tcgacgacaa
      421 ccgcggggcg ctcgagagga cgctgtgcac ccttccggcg ggcgtcgtct cgtacgacca
      481 gaccagcagc tggggcgagt ggttcaacgt ccgcatcgtc gagttcgtcg tcaaggacga
      541 gaacagccgc gagatcgcga gctccggcga gctccagcag cagcgcggga accgcgatcc
      601 gcgtggcgtc gcggtctgcc cgcgcggcga gtcggttcgg aagcagctgc gcgagggcca
      661 cggtcggctc ggcggcctgg aggtacagcc cgaggaggcg cgccttccgg agtggctcga
      721 cacgatcacg ggggccagcc gtggctaggg gccgcctgtc gctcctcgag cgcaaccagc
      781 gcgtgatcgg gacgctcgcg ctgctcgtta tcctcggcgg gaccgcgttc gcgctcctgc
      841 tccagggcgg gttcctcacc cagaagtacc gcgtcacggc gctgttcacg gacgcggccg
      901 gcgtccgcga gggcgacaac ctgacggtcg ccgggctcgt cgcggggcgg gtcgaggcgg
      961 tccgcgtcga gggcggtcag gtcgcgatgg agctcggcgt gcaggaggac gtggggctcc
     1021 cggccgacag ccgcgccgag gtcgtcgtcg agacgctcct cgggcggaag tcggtcgcgc
     1081 tcctccccgg cgattccgag gagccgctcg aggacggcga cgtgatcccg ctcgagcgca
     1141 cgacgacgcc ggtcgacatc ccggagttca acgacatctc ggtcgagctg ctcgaggcgt
     1201 cggacgccga ggcgctcgag acgttcctcg aggacgtgag cgagatcacg aagggcaagg
     1261 cgcaggaggt ccaggcgctg atccgcggtc tcgagcaggt gacggccgcc gtcgacgccc
     1321 gccgcgacga gctcgagggg ctcatcgact cgctccgggt ggtctcgacg acgctcggcc
     1381 agcgcgacca gacgatcgtg tccctgatcg acaacctcga cgtcgtcctc ggcaacctgg
     1441 ccgagcgcca ggatgcgctc gagcggctgc tggtcgcgac ggccggcgcg acgcacgaga
     1501 ccgccgatct cgtccgtcgg aaccgcctcg tcctcgacga cacgctccgg ttcctccacc
     1561 gcgacctcag ggtcctcgat cgccaccaga tcgacatcgc cgccgcgatc tcgtacctgg
     1621 agaacggcgt ccaggggtac cagggcgtcg gctactcgca gtgcacgcgc ccgggcgtcg
     1681 gatgcgtccc gaacgcgtgg gcgaacatct tcatccagtc gctcgggccg ctcggggtcg
     1741 acgcgatcgt cggcgagtgc gggctcgtcg acaagctctt cg
//
LOCUS       DASZOB010000147         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_113178, whole genome shotgun sequence.
ACCESSION   DASZOB010000147 DASZOB010000000
VERSION     DASZOB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113178"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..731
                     /locus_tag="VG709_02450"
     CDS             120..731
                     /locus_tag="VG709_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31968.1"
                     /translation="MQVFHYHLVTSKVRLVEARYLAKLGFDLVARYGWIEDSSHWFEP
                     GKSWEELDELGFRPRLVELRRGSVNVVLQPGHWDVPRVDHLGVALDDDAYLDALERAS
                     DLEMNIQDHPGKRTFIATEAGYRVEVHPPRDWIEEILEGSDDLRLAELHLRAEDPDVK
                     AAALAAIFGTEPADGDVVVGDTLVRFLPGGPSGRPELVGEVLS"
     gene            728..1675
                     /gene="purM"
                     /locus_tag="VG709_02455"
     CDS             728..1675
                     /gene="purM"
                     /locus_tag="VG709_02455"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWC31969.1"
                     /translation="MTGGYSAAGVSLATADAVVDRLRAAADSTRTEAVVSSLGGFAGL
                     YDLDGERLLAASTDGVGTKLILARRAGRLGDAGCDLAAHCVNDGLTTGAEPLFLLDYV
                     AANSLDLAQVAELVDGAASVCREAGCALLGGETAEMPGVYREGEIDFAGTCVGLVHRD
                     ALVDGTRVEDGDVVLGLRSSGLHANGFSLVRRLVGDESFDADLLLAPTRLYLDDVRGL
                     RALCDVHALAHVTGGGIAGNLARVLPEGLGARVDPSSWERPDVYRWLDERGVPEDEQR
                     RVFNLGIGYCAVVPAAEAARAPYPAIGRIEAGVDGVEFD"
BASE COUNT          217 a          610 c          653 g          247 t
ORIGIN      
        1 ggacccgcgg ggcggcggcg cggccggggg cgtcgacatg gcggacggtt cgccgcgccc
       61 tcgaacagtc ctcccgtggg cgggggcccg ccacggcgcc ggttacgatc cgccgggccg
      121 tgcaggtctt ccactaccac ctggtgacga gcaaggtgcg gctcgtcgag gcccgctacc
      181 tcgccaagct cggcttcgac cttgttgcac gctacgggtg gatcgaggac agctcgcact
      241 ggttcgagcc cgggaagtcg tgggaggagc tcgacgagct gggcttccgg ccgcgcctcg
      301 tggagctgcg acgaggcagc gtcaacgtcg tcctgcagcc cggccactgg gacgttccgc
      361 gcgtcgacca cctcggcgtc gcgctcgacg acgacgcgta cctcgacgcg ctggagcggg
      421 cgtccgacct ggagatgaat attcaggatc atccgggcaa acggacgttc atcgcgacgg
      481 aggccggcta cagggtggag gtgcatccgc cgcgcgactg gatcgaggag atcctcgagg
      541 ggtcggacga ccttcggctc gccgagctgc acctgcgcgc ggaggatccc gacgtgaagg
      601 cggccgcgct cgcggcgatc ttcggcaccg agccggcgga cggcgacgtc gtcgtcggcg
      661 acacgctcgt gcgcttcctc cccggtgggc cgagcggccg gccggagctc gtcggcgagg
      721 tgctctcgtg acgggcggct actcggcggc gggcgtgtcg ctcgcgacgg ccgacgcggt
      781 cgtcgatcgg ttgcgcgcgg ccgccgattc cacacggacg gaggccgtgg tctcgagcct
      841 cggcgggttc gcgggcctct acgacctcga cggcgaacga ctcctcgccg cctcgacgga
      901 cggggtcggc acgaagctca tcctggcgcg acgcgccggg cgcctcggcg acgccgggtg
      961 cgacctcgca gcgcactgcg tgaacgacgg cctgacgacc ggcgccgagc cgctcttcct
     1021 gctcgactac gtcgcggcca acagtctcga cctggcgcag gtggcggagc tcgtcgacgg
     1081 cgccgcctcc gtctgccgcg aggccgggtg cgcgctcctc ggcggcgaga cggccgagat
     1141 gcccggcgtc taccgggagg gcgagatcga cttcgccggg acgtgcgtcg gactcgtcca
     1201 ccgcgatgcg ctcgtggacg gcacgcgggt cgaggacggc gacgtcgtcc tcgggcttcg
     1261 ctcgtccggc ttgcacgcga acgggttctc gctcgtgcgc cgcctcgttg gcgatgaatc
     1321 cttcgacgcg gacctcctgc tcgccccgac ccggctctac ctcgacgacg tgcggggcct
     1381 gcgcgcactc tgcgacgtgc acgcgctcgc ccacgtgacg ggaggcggga tcgcggggaa
     1441 cctcgcccgg gtgctgccgg aggggctcgg cgcgcgggtc gacccgtcgt cgtgggagcg
     1501 cccggacgtg taccgctggc tcgacgagcg gggcgtgccg gaggacgagc aacgccgcgt
     1561 cttcaacctc ggtatcggct actgcgccgt cgtgccggcc gccgaggccg cgcgagcgcc
     1621 gtaccccgcg atcggccgga tcgaggcagg agtcgacggt gtcgagttcg actgaggggc
     1681 aggcgcagtg cgccttccgt cacacatccg cgcaggcgtt gcgaccg
//
LOCUS       DASZOB010000148         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_104469, whole genome shotgun sequence.
ACCESSION   DASZOB010000148 DASZOB010000000
VERSION     DASZOB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104469"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VG709_02460"
     CDS             <1..742
                     /locus_tag="VG709_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWC31970.1"
                     /translation="CVERALANVPFLVVQDLTLRGYEDYADAVLPASAFLEKDGHFTD
                     WEGRGQRIHPVRQPPGLARPDWQIFQELSEVMGKDMGFRSLDHLWREMGELLAGQGRD
                     EEGSSTGASPEEVVAQRAGGSRASSEGDATGDDTLTLFTYPLLVDEGRLSVDADELKA
                     ALEEQPFVEVHPEDAERLALGDGVKAVLRNGMGEATLPVRVSDGVAPGSAFVPWNQPG
                     FRANEMFERGFTTSVTVEAAERTEEGAA"
     gene            742..1911
                     /gene="nuoH"
                     /locus_tag="VG709_02465"
     CDS             742..1911
                     /gene="nuoH"
                     /locus_tag="VG709_02465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWC31971.1"
                     /translation="MDWLDWILLLARVVIVFVALLVLVMLVVWVERKVVADMQTRLGP
                     RRAGPFGVLITLADGIKLFFKEGVTPVQAERPVYVLAPVLSLIPALLAFAVVPFGTGV
                     TLFGRHVPFQLADLNVGVLFFLAMGSLAIYGIVLAGWASGSAYPLLGGIRSSAQMISY
                     EVGMGLGLVAVLMYAGTLQMSEIVARQGGALEFFGTGVPVFPRWNIVPQLPAFLIFMY
                     AALAETNRPPFDMPEAESELVAGYHTEYSGIRFAMFFLAEYLHMITISAVAVTLFLGG
                     WRGPIFGFLPWLWPTVWFFLKVALLVFLFVWVRATIPRIRYDRLMRFGWKVVIPFSLL
                     WILLTGAIVVIPDVYGRDALFRWLAIGAGVVLLLSLVWPLFSRDREAPHAVRSEG"
     gene            1911..>2212
                     /locus_tag="VG709_02470"
     CDS             1911..>2212
                     /locus_tag="VG709_02470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWC31972.1"
                     /translation="MGFVDFLKGLGVTFKNMFRPETTVEYPKQVPEMYPRTHVGRHLL
                     NRHENGLEKCIGCELCAWACPADAIYVQGADNDPDTPVSAGERYAETYQINYLRCI"
BASE COUNT          298 a          714 c          795 g          405 t
ORIGIN      
        1 gtgcgtcgag cgggcgctgg cgaacgtgcc gttcctcgta gtgcaggacc tcacgctccg
       61 cggctacgag gactacgcag acgcggtgct tccggcgtcg gcgttcctcg agaaggacgg
      121 gcacttcacg gattgggagg gtcgcgggca gcggatccac ccggtgcgcc agccgccggg
      181 gctcgcgagg ccggactggc agatcttcca ggagctgtcg gaggtcatgg ggaaggacat
      241 gggcttccgc tccctcgacc acctctggcg cgagatgggc gagctcctcg ccgggcaggg
      301 aagagacgaa gaggggtcgt cgacgggcgc gtctccggag gaggtagtcg cgcagcgagc
      361 cggaggctcg cgagcatcct ccgaaggaga cgcgaccggc gacgacacgc tgactctgtt
      421 cacgtacccg ctgctggtgg acgaggggag gttgtcggtc gacgccgatg agctgaaggc
      481 ggcgctcgaa gagcagccgt tcgtcgaggt gcaccccgag gacgccgagc gtctcgcgct
      541 cggggacggc gtcaaggccg tcctccgcaa cgggatgggg gaggcgacgc taccggtgcg
      601 cgtgtcggac ggcgtcgcgc ccgggtcggc gttcgttccg tggaaccagc cgggcttccg
      661 cgcgaacgag atgttcgaac gcgggttcac gacgagcgtg acggtcgagg ccgcggagcg
      721 cacggaggag ggggcggcct agtggactgg ctcgactgga tcctgctctt ggcccgcgtc
      781 gtgatcgtgt tcgtcgcgct gctcgtcctc gtcatgctcg tggtctgggt cgagcggaag
      841 gtcgtcgccg acatgcagac gcgcctcggg ccgcgccgtg ccgggccgtt cggggtcctc
      901 atcacgctcg ccgacggcat caagctgttc ttcaaggagg gcgtcacgcc ggtccaggcc
      961 gagcgtccgg tgtacgtcct cgcgccggta ctgtcgctga tcccggcgct cctcgcgttc
     1021 gccgtcgtgc cgttcggtac cggcgtcacc ctgtttgggc gtcacgtgcc gttccagctt
     1081 gccgacctca acgtcggcgt cctgttcttc ctcgcgatgg gatcgctcgc gatctacggg
     1141 atcgtcctcg ccgggtgggc gtcgggctcg gcctacccgc tgctcggcgg gatccgctca
     1201 agcgcgcaga tgatctcgta cgaggtcggg atggggctcg ggctcgtcgc ggtgctgatg
     1261 tacgcgggga cgctccagat gtcggagatc gtggcgcgtc agggcggcgc gctggagttc
     1321 ttcgggaccg gcgttccggt gttcccgagg tggaacatcg tgccgcagct ccccgcgttc
     1381 ctgatcttca tgtacgcggc gctcgcggag acgaaccgtc caccgttcga catgcccgag
     1441 gccgagagcg agctcgtcgc cgggtaccac acggagtact ccgggatccg cttcgcgatg
     1501 ttcttcctgg ccgagtacct ccacatgatc acgatctcgg cggtcgccgt gacgctgttc
     1561 ctcggcgggt ggcgtggtcc gatcttcggc ttcctcccgt ggctctggcc gaccgtgtgg
     1621 ttcttcctga aggtcgcact gctcgtgttc ctgttcgtct gggtccgcgc gacgatcccg
     1681 cggatccgct acgaccggct gatgcgcttc ggatggaagg tcgtgatccc gttctcgctg
     1741 ctctggatcc tgctcacggg ggcgatcgtc gtgatccccg acgtctacgg ccgcgatgcg
     1801 ctgttccgct ggctcgcgat cggcgcgggc gtcgtgctgc tgctgtcgct cgtctggcca
     1861 ctgttctcgc gggaccgcga ggccccgcac gccgtcagga gtgagggctg atgggcttcg
     1921 tcgacttcct caagggcctc ggggtcacgt tcaagaacat gttccggccg gagacgacgg
     1981 tcgagtaccc gaagcaggtt ccggagatgt acccgcgcac gcatgtcggg cggcatctcc
     2041 tcaaccgtca cgagaacggc ctcgagaagt gcatcgggtg cgagctgtgc gcgtgggcgt
     2101 gcccggccga tgcgatctac gttcagggcg ccgacaacga ccccgacacg ccggtgtcag
     2161 caggcgagcg gtacgccgag acgtaccaga tcaactacct ccgctgcatc at
//
LOCUS       DASZOB010000149         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_164755, whole genome shotgun sequence.
ACCESSION   DASZOB010000149 DASZOB010000000
VERSION     DASZOB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164755"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VG709_02475"
     CDS             <1..979
                     /locus_tag="VG709_02475"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HWC31973.1"
                     /translation="LVRVLPPDNIPAIDEPAFVGVGEAAGWLDDREPVVSVGVGGDAR
                     AYPVQILTWHEIVNDEIGGRPVAVTYCPLCNSAVAFERDLGGRVLEFGTSGQLYESAL
                     VMYDRPTQSLWTHFTGQAISGELKGTTLQVIPAQMLSFGEWRTLHPDGLVLSRGTGFE
                     RPYGENPYEGYDRGGPYDSFVSGEADDRLPAIARVVGVETDGEAVAYPYSSLRAERGA
                     SVLHDTVAGRRIAVLWKAGVASAHDAREIRAGRDVGSSGVFVASVNGRSLTFAAAGGR
                     IEDGETGSEWSLSGRAVSGPLAGTQLEPVAHLDTFWFAWAAYHPGTEVL"
     gene            complement(974..1429)
                     /locus_tag="VG709_02480"
     CDS             complement(974..1429)
                     /locus_tag="VG709_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31974.1"
                     /translation="MTVGTIAGAIGKGLVAGAAGTVAMTISSTLEMKARGREQSTAPA
                     EAAERVLGVEPKGESEETRLNTLVHFGYGTGWGIPRGLLGLVMSGPMATATHFLAVWG
                     TALGMLPGLGVAPPPTQWGAKEVAVDAWHHAVYAVVTGLVFDLLDRDRS"
BASE COUNT          224 a          544 c          542 g          231 t
ORIGIN      
        1 cctcgtgagg gtgctgccgc ccgacaacat cccggcgatc gacgagccgg cgttcgtcgg
       61 cgtcggcgag gcggcggggt ggctcgacga tcgggagccg gtcgtctccg tcggggtcgg
      121 cggcgacgcg cgcgcgtacc ccgtccagat cctcacgtgg cacgagatcg tcaacgacga
      181 gatcggcggg cggcccgtcg cggtgacgta ctgcccgctc tgcaactccg cggtcgcctt
      241 cgagcgcgac ctcggcggca gggtgctcga gttcgggacg tcgggacagc tctacgagag
      301 cgcactcgtg atgtacgacc gtccgacgca gtcgctctgg actcacttca ccggtcaggc
      361 gatcagcggc gagctcaagg ggacgacgct ccaagtcatc cccgcgcaga tgctctcgtt
      421 cggcgagtgg cggacactgc atccggacgg gctcgtgctc tcacgcggta ccggcttcga
      481 acgcccctac ggcgagaacc cctacgaggg atacgacagg ggcgggccgt acgactcttt
      541 cgtcagcggt gaggcggacg atcggctccc cgcgatcgcg cgcgtcgtcg gcgtcgagac
      601 ggacggcgag gccgtggcgt atccgtactc atcgctccgg gccgagcgcg gggcctccgt
      661 cctccacgac accgtcgccg gaaggcggat cgccgtgtta tggaaggcgg gggtggcgtc
      721 ggcgcacgac gcgcgggaga tcagagccgg gagagacgtc ggatcgtccg gcgtcttcgt
      781 cgcgtctgtg aacggacgga gcctgacgtt cgccgccgca ggcggacgga tcgaggacgg
      841 cgagactgga tcggaatggt ccctctccgg ccgggcggtc tccgggccgc tggcggggac
      901 gcagctcgag ccggtcgcgc acctcgacac gttctggttc gcgtgggccg cgtaccaccc
      961 cggtacagag gtcctatgag cggtcgcggt cgaggaggtc gaagacaagg ccggtcacga
     1021 ccgcgtagac cgcgtggtgc cacgcgtcga ccgccacctc cttcgccccc cactgcgtcg
     1081 gcgggggggc aaccccgagc cccggaagca tgccgagcgc ggttccccag accgcgagga
     1141 aatgcgtcgc ggtcgccatc gggccggaca tcacgagccc gagcaggccc cgtgggatcc
     1201 cccacccggt cccgtacccg aagtggacga gcgtgttgag ccgcgtctcc tccgactcgc
     1261 ccttcggctc gacgccgagg accctctcgg cggcctcggc cggagccgtc gactgttccc
     1321 ggcccctggc tttcatctcg agcgtgctcg agatcgtcat cgcgaccgtc cctgcggctc
     1381 ccgcgacgag ccccttgccg atcgcgccgg cgatcgttcc gaccgtcatc ctcgccttcc
     1441 tttcccgcag aaccgcgact gcatacgaca gttgccctcc gattgccggc tcggagccga
     1501 actgtcatcg cgggcacaca cgagaaggcg gggcggagga t
//
LOCUS       DASZOB010000150         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_23430, whole genome shotgun sequence.
ACCESSION   DASZOB010000150 DASZOB010000000
VERSION     DASZOB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23430"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VG709_02485"
     CDS             <1..954
                     /locus_tag="VG709_02485"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HWC31975.1"
                     /translation="GLESIRDAGIREVGIVVGDTADEIAAAVGDGSAWGLEVSYIGQD
                     QPLGLAHAVVVSRGFLGDDDFVMYLGDNVLLEGIGRFVDGFTRHRPNAQIFLAAVPEP
                     ERFGVAVLEGDRVTRLVEKPKEHISDLALVGVYLFDATVHEAIDTIQPSWRGELEITD
                     SIQALIDGGRTVRAEMVSGWWKDTGHLEDLLEANRMLLSTLEPRMEGDVDGRSRLVGN
                     VVIERGAKVSRSVIRGPAIVGEDTIVEDSLIGPNASIYYGCRISASEIQDSIVMERCR
                     IEEVRGLTGSLLGKDVEVRRGKDRPAMYRVMLGDQSQVELF"
     gene            complement(1030..2730)
                     /locus_tag="VG709_02490"
     CDS             complement(1030..2730)
                     /locus_tag="VG709_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31976.1"
                     /translation="MSYRAPQMEVQMPVSRSRSVLLVVALVGLLALPAAVEAHPPSSG
                     SSGSAPRSGPATGSLPGTTGPCTKQAAKSMPDGRGHDHLDITQHRFQCNARLAFFDPL
                     LDELDEGIFGAQDAILGEIDIENDVLAIAVAFPESGVLFYDVQNPAEPEFIGWYRSSQ
                     CEELVFDVDCGAYVDLSEDGTVAFLSLQDISVLPQQPPDAGVLPVTVPGVEVIDVDSP
                     VGPTRTQIYPLASGIGGTHTSNSHVIPADADDTDPRAPGEYLFSVKNVNALHISEVNR
                     VGGVPFLTPVSEIELDELHDMYIDNDPLTGRTYVYIAGGFATGFYVYDVTDPAQPEFL
                     GEWDPTPECAEDWYGHTTFTVVRNGRRYVTIDAELFGGGGEFGEQSAEDQAEGCGRLV
                     GNGDRPGPLWIVDATNFENLQRGANPTDGEESGQVAQELKQASLEALVSTWTNPAGRA
                     AGNIEFSPHNQQVVGNRIYLSHYHGGVYQLDATAAFAGESTRPREMAFVVPHNEPIRP
                     LYEPMITDPLIPFIAEFFQARPTVWDTLFYKGFVIIVDSTGGLYSYKFGKAAAQRQKG
                     "
     gene            2832..4319
                     /locus_tag="VG709_02495"
     CDS             2832..4319
                     /locus_tag="VG709_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31977.1"
                     /translation="MKLRVAALASLLGLLGSSLGSAQAIAETSCPAPKTRMKFAPQTY
                     IDTDFAGGEPTVEAHPDGTLFYGAHAGTTHIFAPAAADEDSTAFVEHYTNQTYIWVSH
                     DDGKTWKFIPRFMPPDNVPGTGFSDPDFAIDKAGQVYASEINLVNVAMSKADDPQHED
                     FTLQNFFAQTITDRQWSEADQRDVLYLVGNAFGGGTFPTKPAGNFGHFLYKSTDGGRT
                     FSPGDPDGAGLGDLQVDKRNGTLYEAWYDDDVLSMAAFRDARSNPFTTNVQPDLNEIA
                     RNVDMNFTAHWPSLDVDDHGNVYIVWSEDGNDGTGARPGGIWYSYSTDAGNTWAAPIQ
                     VDSDRDGTDIWPWLAVGAPGRVAISWLEAPGEPLPGNDPEDAGGSPLSTADDRPWVIE
                     AAQTLNGLGCRSSSVPGFRTVVATPDPVHRGTICRQGTICQAQLIDRRMGDYFSIEIN
                     EQGHMFAGYSDTRQGGMVALPGYVRQVGGPVFQKPPSTRVKAARR"
     gene            4368..>4872
                     /locus_tag="VG709_02500"
     CDS             4368..>4872
                     /locus_tag="VG709_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31978.1"
                     /translation="MRVAIDARPAVARGKTGVGWYASHLIRRLPFVDPETTYLAWYLY
                     ARRILGKPLFFTDVHAPNFEERWTPFPARLFWRLSRREFPRVEWLVGFDVLFATNFVP
                     PPTRSRRVVLAVHDLAFRRHPTSVPAPTREWLDARLRPSLDRGSAVERGTEPCVEPLA
                     GRRRDGCW"
BASE COUNT          767 a         1641 c         1703 g          761 t
ORIGIN      
        1 gggctcgagt cgatccgcga tgctggcatc cgcgaggtcg ggatcgtcgt cggcgacacc
       61 gccgacgaga tcgcggccgc ggtcggggac gggtcggcgt gggggctcga ggtgtcgtac
      121 atcggccagg accagcccct cgggctcgcg cacgccgtcg tggtctcacg cgggttcctc
      181 ggcgacgacg acttcgtcat gtatctcggc gacaacgtgc tgctcgaagg catcggccgg
      241 ttcgtcgacg gcttcacgcg gcaccgtccg aacgcgcaga tcttcctcgc tgcggtcccg
      301 gagccggagc gcttcggcgt cgcggtgctc gagggcgacc gggtcacgcg gctcgtcgag
      361 aagccgaagg agcacatctc cgatctcgca ctcgtcggcg tctacctctt cgacgcgacg
      421 gtccacgagg cgatcgacac gatccagccg tcgtggcgcg gcgagctcga gatcaccgac
      481 tcgatccagg cgctgatcga cggcggacgg acggtccgcg cggagatggt gagcggctgg
      541 tggaaggaca ccggccacct cgaggacctc ctcgaggcga accgcatgct gctctcgacg
      601 ctcgagccgc ggatggaggg cgacgtcgac ggtcggtcgc gcctcgtcgg caacgtcgtg
      661 atcgagcgcg gcgcgaaggt ctcgcgctcc gtgatccgcg gcccggcgat cgtcggcgag
      721 gacacgatcg tcgaggactc gctgatcggt cccaacgcct cgatctacta cgggtgccgg
      781 atcagcgcca gcgagatcca ggactcgatc gtgatggagc gctgcaggat cgaggaggtc
      841 cggggcctca cggggtcgct gctcggcaag gacgtcgagg tccgccgcgg caaggaccgc
      901 cccgcgatgt accgggtcat gctcggcgac cagagccagg tggagctctt ctaatctcgt
      961 cgagccgctc ggaaagggga caggggagac gccctgggcg tctcccctcc cgttgagctg
     1021 ccgtagcgtc tagcccttct ggcgctgggc tgccgccttt ccaaacttgt acgagtacag
     1081 gccgccggtc gagtcgacga tgatcacgaa gcccttgtag aagagcgtgt cccacaccgt
     1141 cgggcgggcc tggaagaact cggcgatgaa cgggatcagg gggtcggtga tcatcggctc
     1201 atacagcggc ctgatcggct cgttgtgtgg aacgacgaac gccatctcgc gcggccgggt
     1261 cgactcgccg gcgaacgccg cggtggcatc gagctggtac acgccgccgt ggtaatgcga
     1321 gaggtagatc cggtttccga ccacctgctg gttgtgcggc gagaactcga tgttcccggc
     1381 cgcacgcccg gctgggttcg tccacgtgga cacgagcgcc tcgagggagg cctgcttcag
     1441 ctcctgggca acctgaccgc tctcctcgcc gtcggtcggg ttcgcgccgc gctgcaggtt
     1501 ctcgaagttc gtcgcgtcga cgatccagag cgggccgggt ctgtcgccgt tccccaccaa
     1561 tcggccacag ccctcggcct gatcctctgc ggactgctcg ccgaactcgc cgcctccgcc
     1621 gaagagctct gcgtcgatcg tcacgtagcg gcgtccgttc cggacgaccg tgaaggttgt
     1681 gtggccgtac cagtcctccg cgcactctgg cgtggggtcc cactcgccga ggaactcagg
     1741 ctgggccggg tccgtgacgt catagacgta gaaccccgtc gcgaacccgc cggcgatgta
     1801 gacgtacgta cggcccgtca gagggtcgtt gtcgatgtac atatcgtgga gctcgtcgag
     1861 ctcgatctcg ctcacgggag tgaggaaggg aacgcctcca acacgattga cctcggagat
     1921 gtgcagcgcg ttgacgttct tcaccgagaa gaggtactcg cccggcgccc gggggtctgt
     1981 gtcatcggcg tccgccggga tgacgtgcga gttcgacgtg tgggtccctc cgatgcccga
     2041 ggcgagcggg tagatctggg tgcgcgtcgg cccgaccggg ctgtcgacgt cgatcacctc
     2101 gacgcccggg acggtcaccg gcaggacccc ggcgtccggc ggctgctggg gcaggacgct
     2161 gatgtcctgc agcgagagga acgcgaccgt cccgtcctcc gagaggtcga cgtacgcgcc
     2221 gcagtcgacg tcgaagacga gctcctcgca ctgcgagctg cggtaccagc caatgaactc
     2281 cggctcggcc gggttctgga cgtcgtagaa gaggaccccc gactcgggga acgccaccgc
     2341 gatcgcgagc acgtcgttct cgatgtcgat ctcgccgagg atcgcgtcct gtgccccgaa
     2401 gatcccctcg tcgagctcgt ccagcagtgg gtcgaagaag gcgaggcgcg cgttgcactg
     2461 gaaccggtgc tgagtgatgt cgaggtggtc gtggcccctc ccgtccggca tcgacttcgc
     2521 ggcctgcttc gtgcagggtc cggtcgtccc ggggagcgac ccggtggccg ggcccgatcg
     2581 cggcgccgag ccggaactcc ctgacgaagg aggatgggcc tcgaccgcgg ccggaagcgc
     2641 gagtaggccg acgagcgcga cgacgagcag gacactgcgg gaacgggaca cgggcatctg
     2701 tacctccatc tggggggctc gatagctcat tcgtcacgga tgggacgatt cctgccacca
     2761 ggccggcggt ccaggggagg aatcgcgcgg cggccgccga aagtacccat aacggcacta
     2821 ggaggcccgc catgaagctt cgcgtcgctg ccctcgcctc gctcctcggt cttctcggga
     2881 gctccctcgg ctcggcgcag gcgatcgccg agacctcgtg cccggcccca aagacacgta
     2941 tgaagttcgc tccgcagacc tacatcgaca ccgacttcgc gggcggcgag ccgacggtcg
     3001 aggcgcatcc cgacgggacg ctgttctacg gcgcgcatgc cggtacgacg cacatcttcg
     3061 cccccgcggc ggccgacgag gactcgaccg cgttcgtcga gcactacacg aaccagacgt
     3121 acatctgggt gagccacgac gacggcaaga cgtggaagtt catcccgcgg ttcatgccgc
     3181 cggacaacgt gcccggcacg gggttctccg acccggactt cgcgatcgac aaggcgggcc
     3241 aggtgtacgc gtcggagatc aacctcgtga acgtcgcgat gtcgaaggcg gacgacccgc
     3301 agcacgagga cttcacgctc cagaacttct tcgcgcagac gatcacggac cggcagtggt
     3361 ccgaggccga ccagcgcgac gtgctctacc tcgtggggaa cgcgttcggg ggcgggacgt
     3421 tcccgacgaa gcccgccgga aacttcgggc acttcctcta caagagcacc gacggcggac
     3481 gcacgttctc accgggcgat ccggacggtg ccgggctcgg cgatctccag gtcgacaagc
     3541 ggaacggaac tctgtacgag gcctggtacg acgacgacgt gctctcgatg gcggccttcc
     3601 gcgatgcgcg gagcaacccg ttcacgacga acgtccagcc ggacctgaac gagatagcgc
     3661 gaaacgtcga catgaacttc acggcccact ggccgtcgct cgacgtcgac gaccacggca
     3721 acgtctacat cgtctggagc gaggacggga acgacggtac cggcgctcgt ccgggaggga
     3781 tctggtactc gtactcgacg gacgccggga atacatgggc cgctccgatc caggtcgact
     3841 ccgaccggga cggcaccgat atctggcctt ggctcgcggt cggggcacct ggaagggtcg
     3901 cgatctcgtg gctcgaggcg ccgggtgagc cgcttcccgg gaacgacccc gaggatgccg
     3961 gcgggtcccc gctctcgacg gccgacgacc ggccgtgggt gatcgaggcc gcgcagacgc
     4021 tcaacgggct cggatgccgc tcctcgagcg tgcctggctt ccgcacggta gtggcgacgc
     4081 ccgaccccgt ccaccgcggc acgatctgcc gacagggaac gatctgccag gcgcagctga
     4141 tcgaccgtcg gatgggcgac tacttctcga tcgagatcaa cgagcagggt cacatgttcg
     4201 ccggctactc cgacacgcgg cagggaggca tggtcgcgct cccgggctac gtgcggcagg
     4261 tcggcggccc cgtcttccag aagccgccgt cgacgcgagt gaaggcggcg cgccggtagc
     4321 gcccgtcgct ccgcaccgcc ggcgatcagg cgggtagggt cccggccatg cgcgtggcga
     4381 tcgatgcgcg gccggcggtg gcgcgcggga agaccggcgt cgggtggtat gcgtcgcacc
     4441 tgatccggcg gctcccgttc gtcgacccgg agaccaccta cctggcgtgg tacctctacg
     4501 cgcgccgcat cctcggaaag ccgctgttct tcacggacgt ccatgcgccg aacttcgagg
     4561 agcggtggac gccgttcccg gcgcgcctgt tctggcggct gtcgcggcgc gagttcccgc
     4621 gcgtcgagtg gctcgtcggc ttcgacgtgc tgttcgcgac caacttcgtg ccgccgccga
     4681 cgcggagccg ccgggtcgtg ctcgccgtgc acgatctcgc gttccggcgc catccaacat
     4741 ccgtcccggc gccgacccgc gagtggctcg acgcacggct ccgtccctcg ctcgaccgcg
     4801 gcagcgcggt cgagcgaggg acggagccgt gcgtcgagcc actcgcgggg cggcgccggg
     4861 acggatgttg ga
//
LOCUS       DASZOB010000151         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_195488, whole genome shotgun sequence.
ACCESSION   DASZOB010000151 DASZOB010000000
VERSION     DASZOB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195488"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /gene="tsaB"
                     /locus_tag="VG709_02505"
     CDS             complement(<1..321)
                     /gene="tsaB"
                     /locus_tag="VG709_02505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWC31979.1"
                     /translation="MLVLAIETATPQTSVAIGTEQGVIASASLSWARGHGEVLVPAIE
                     RLQAWSEVRLAQVGGVAVGIGPGLFTGLRVGVSTARALAHVLGVAVVGIGSLDVLAFG
                     VRHTR"
     gene            complement(322..810)
                     /gene="tsaE"
                     /locus_tag="VG709_02510"
     CDS             complement(322..810)
                     /gene="tsaE"
                     /locus_tag="VG709_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWC31980.1"
                     /translation="MLLGPLSFRTASAEETTDVGAAIGALLGAGDVVSLTGDLGAGKT
                     TLVRGAARALDVEEAVASPTFVLVREYHGTLPVYHVDVYRLDRLQDVIDLGFEDLMDG
                     SGVVFIEWGNAVEALLPRSYLEVELEIEEGGRRVVVSARGPMWAGRWERVEAALATWG
                     AT"
     gene            complement(817..1428)
                     /locus_tag="VG709_02515"
     CDS             complement(817..1428)
                     /locus_tag="VG709_02515"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWC31981.1"
                     /translation="MSSSLEDVRAEALACTKCRLSAGRTQVVFADGSPGADLMFVGEG
                     PGYHEDVQGLPFVGAAGQLLNRLLGEIGLSREDVYITNVVLCRPPGNRDPLPDELESC
                     RPYLLRQIELVDPKVIVTLGNFATRVILDRPVSISRVRGTKSSFHGRLVIPTFHPAAV
                     LRSGGDASAPMRAIREDLRVVREALDEARVTAPAVPEEQLGLF"
     gene            complement(1425..>1742)
                     /locus_tag="VG709_02520"
     CDS             complement(1425..>1742)
                     /locus_tag="VG709_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016326458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWC31982.1"
                     /translation="VGEDGSVLAGASPGGEEEMRPPWPGANTTLVVVGTDAKLSRDRA
                     HLLAVAAHDGVARAVRPAHTIWDGDTVFSLATGVVEAAQPLVERMAEGVVAEAIRSAV
                     GEG"
BASE COUNT          251 a          678 c          594 g          219 t
ORIGIN      
        1 cctcgtgtgg cggacgccga acgcgaggac gtcgagcgag ccgatcccga cgacggccac
       61 gccgaggacg tgcgcgaggg cacgggccgt tgataccccc acccggagcc cggtgaacag
      121 cccgggcccg atcccgacgg cgacgccgcc gacctgagcg agccggacct cggaccacgc
      181 ctggagccgc tcgatcgccg ggaccaggac ctctccgtgc ccgcgcgccc acgagagcga
      241 cgccgacgcg atcacgccct gctcggtgcc gatcgcgacc gacgtctgcg gcgtcgcggt
      301 ctcgatcgcg aggaccagca cctacgtggc cccccatgtc gcgagcgccg cctcgacgcg
      361 ttcccagcga cccgcccaca tggggccgcg cgcggagacc accacgcggc gacccccctc
      421 ctcgatctcg agctcgacct cgaggtacga ccgcgggagc agcgcctcga ccgcgttccc
      481 ccactcgatg aacacgaccc cggagccgtc catgaggtcc tcgaacccga ggtcgatcac
      541 gtcctgcagc cggtcgagcc ggtagacgtc gacgtggtac acgggcagcg tgccgtggta
      601 ctcgcggacc agcacgaagg tcggcgaagc gacggcctcc tcgacgtcga gcgcccgcgc
      661 cgctcccctc acgagcgtcg tcttccccgc tccgaggtcg ccggtgaggc tcacgacgtc
      721 gccggcgccg agcagcgccc cgatggcggc gccgacgtcc gtcgtctctt ccgcggatgc
      781 cgtccgaaag gagaggggac cgagcagcat gtccgctcag aacaggccga gctgctcctc
      841 gggtaccgcc ggcgcggtga ctcgagcctc gtcgagcgcc tcgcggacca cccgcaggtc
      901 ctcgcggatc gcgcgcatcg gcgcgctcgc gtcgccgccg ctccgcagca ccgccgcggg
      961 atggaacgtg gggatcacga gccgcccgtg gaacgagctc ttggtgccgc ggacgcgcga
     1021 gatcgacacc gggcggtcca ggatcacgcg cgtcgcgaag ttcccgagcg tcacgatcac
     1081 cttcggatcc acgagctcga tctgcctgag caggtacggc cggcacgact cgagctcgtc
     1141 gggcagcggg tcccggttcc ccggaggccg gcacaggacg acgttcgtga tgtacacgtc
     1201 ctcgcggctg aggccgatct cgccgagcag gcggttcagg agctgtccgg cggcgccgac
     1261 gaacgggagg ccctggacgt cctcgtggta gccggggccc tcgccgacga acatgaggtc
     1321 ggcacccggg ctgccgtcgg cgaacacgac ctgcgtgcgg cccgcggaca ggcggcactt
     1381 cgtgcacgcg agcgcctccg cgcgaacgtc ctcgaggctc gagctcatcc ttcccccacc
     1441 gcgctccgga tcgcctccgc gacgaccccc tcggccatgc gctcgaccag cggctgtgcg
     1501 gcttcgacga cgcccgtcgc gaggctgaac acggtgtcgc cgtcccagat tgtatgcgcg
     1561 ggcctcaccg cgcgcgccac gccgtcgtgc gcggcgactg cgagcaggtg ggccctgtcc
     1621 ctcgagagct tcgcgtcggt cccgacgacg accagcgtcg tgttggcgcc cggccacgga
     1681 ggtcgcatct cctcctcgcc gcccggcgac gcgccggcga gcacggagcc gtcctcgccg
     1741 ac
//
LOCUS       DASZOB010000152         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_32025, whole genome shotgun sequence.
ACCESSION   DASZOB010000152 DASZOB010000000
VERSION     DASZOB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32025"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VG709_02525"
     CDS             <1..347
                     /locus_tag="VG709_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWC31983.1"
                     /translation="YLLPRLVGYGKALELSISGDRIDAEEAQRIGLVSRVVDDDAFAS
                     ELESYAARLAELPTRAIGATKRVMREALAMSLDETLEREAEVQTELGASADFSEGVAA
                     FLEKRPPRFTGR"
     gene            446..>1867
                     /locus_tag="VG709_02530"
     CDS             446..>1867
                     /locus_tag="VG709_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC31984.1"
                     /translation="MTPSQKRWTLVAVILGSGIVFLDTSVVNLALKAIGEELPRSFVG
                     KVEGQTYVAAGYFVTLSALLVLAGALNDYYGRRRMFALGLAGFLATSVLCAVAPTLEA
                     LIAFRILQGATGALVVPGSLAIITASVEGEEQGRACGIWAGASALTTILGPLVGGILV
                     NSISWRAAFFINVPFLLVAIYACIRKVPESRDEEATGHFDWLGSLVVAVAVGGLAFGT
                     IRGQQTEWRGTEAWAALVIGGVAAVAVPVMMAKRRNPLVPPALFRSRNFTVTNISTFV
                     IYGALYVFLTYQGLFLIGTLGYNEPAAGVAGLPTTALLALFSSRFGRLAARYGPRLFM
                     TAGPAVMAIGLLWLARIPATSEAWVLGGDPGESFLPPGDYFVDLFPAFVVFGAGLMMM
                     VAPLTTALMNSIPRRNAGVGSAINNAISRVGSPLATALIFVAIVSSFYSAIEARVPGV
                     DTSAPRFRDEGAALNPPPEGTSEE"
BASE COUNT          231 a          658 c          673 g          305 t
ORIGIN      
        1 actacctgct gccgcggctg gtcggctacg gcaaggcgct cgagctctcg atcagcgggg
       61 accggatcga cgccgaggag gcgcagcgga tcgggctcgt ctcccgcgtc gtggacgatg
      121 acgcgttcgc gtcggagctc gagtcctacg ccgcgcggct ggccgagctt ccgacccggg
      181 cgatcggggc cacgaagcgc gtgatgcgcg aggcgctcgc gatgtcgctc gacgagacgc
      241 tcgagcgcga ggccgaggtc cagaccgagc tgggagcgag cgccgacttc tccgagggcg
      301 tggcggcgtt cctcgagaag cgcccgccgc gcttcaccgg gcgctgaggc cggccgacgg
      361 gcattcaccg cgacctcgtg gccgggaatc ccaggctgcc cggcgttgtt cccccgcgca
      421 gacgacgacc gaccgggagg ccgacatgac gccctcgcag aagcgctgga ccctggtcgc
      481 ggtgatcctg gggtccggga tcgtcttcct cgacacctcg gtggtcaacc tcgccctgaa
      541 ggcgatcggc gaggagctgc cgcggagctt cgtcgggaag gtcgagggac agacctacgt
      601 cgccgccggc tacttcgtca cgctgtccgc gctgctcgtc ctggccggcg cgctgaacga
      661 ctactacggc cgccggcgga tgttcgcgct cgggctcgcc gggttcctcg cgacgtccgt
      721 cctctgcgcc gtcgcgccga cgctcgaagc gctgatcgcg ttccgcatcc tgcagggggc
      781 gaccggcgcc ctcgtcgtgc ccggatcgct cgcgatcatc accgcgtcgg tcgagggcga
      841 ggagcagggg cgcgcctgcg ggatctgggc gggcgcgtcg gcgctgacga cgatcctcgg
      901 cccgctcgtc ggcggcatcc tcgtgaacag catctcctgg cgggccgcct tcttcatcaa
      961 cgtcccgttc ctgctcgtgg cgatctacgc gtgcatccgc aaggtgccgg agtcgcgcga
     1021 cgaggaggcg accggtcact tcgactggct gggctcgctg gtcgtcgcgg tcgccgtcgg
     1081 cggcctcgcg ttcggaacca tccgcgggca gcagaccgag tggcggggaa ccgaggcgtg
     1141 ggcggcgctc gtgatcggcg gcgtcgcggc ggtcgccgtg ccggtgatga tggcgaagcg
     1201 ccggaacccg ctcgtgcctc ccgcgctctt ccggtcgcgg aacttcaccg tcacgaacat
     1261 ctcgaccttc gtcatctacg gcgcgctgta cgtcttcctc acgtaccagg gcctgttcct
     1321 gatcggcacg ctcggctaca acgagccggc ggcgggcgtc gcgggactcc cgacgacggc
     1381 cctgctcgca ttgttctcgt cgaggttcgg gaggctcgcc gcgcggtatg gaccgcgcct
     1441 cttcatgacc gcgggaccgg ccgtgatggc gatcggcctc ctgtggctgg cgcggatccc
     1501 ggcgaccagc gaggcatggg tcttgggcgg agacccgggt gagtcgttcc ttccgcccgg
     1561 cgactacttc gtcgatctgt tcccggcgtt cgtcgtgttc ggggccgggc tgatgatgat
     1621 ggtcgcgccg ctgacgacgg ccctgatgaa ctcgatcccg aggcggaacg ccggcgtcgg
     1681 ctcggcgatc aacaacgcca tctcgcgcgt cggctctccg ctcgcgaccg cgctgatctt
     1741 cgtcgcgatc gtctcgagct tctactccgc gatcgaggcc cgcgtgcccg gcgtcgacac
     1801 gtcggcgccc cggttccggg acgagggcgc ggcgctgaac ccgccgcccg aggggacgtc
     1861 cgaggag
//
LOCUS       DASZOB010000153         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132934, whole genome shotgun sequence.
ACCESSION   DASZOB010000153 DASZOB010000000
VERSION     DASZOB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132934"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1489
                     /locus_tag="VG709_02535"
     CDS             302..1489
                     /locus_tag="VG709_02535"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWC31985.1"
                     /translation="MNLDPNVHEVLRYPKRTLEVSIPVRMDDGSVRVFKGYRVHHNTA
                     RGPSKGGIRYHPSVHLDEVKALAMWMTWKCAVVGIPYGGAKGGVVVNPKQLSTRESER
                     LTRRYASEILPLIGPERDIPAPDVGTDEQVMAWIMDTYSMNQGFSVPGVVTGKPLSVG
                     GSAGRRASTARGVMYITVATLKHLGMPVDEARVVVQGYGKVGRPVVELLADQGCRIVG
                     VGDVAGGVYNPRGLSPVGLEAHHEQAGTVAGFDGGERLTGEELLEAECDVLIPAALER
                     VVTDENADRVKARVIVEAANGPVTPEADEIFHDAGVHVVPDVLANAGGVTVSYFEWVQ
                     DLQAYFWSEDEVNARLRAVMEDAYIDVLDLAEERKVTMRQAATILGVQRVAEAHRTRG
                     LYP"
     gene            1564..1809
                     /locus_tag="VG709_02540"
     CDS             1564..1809
                     /locus_tag="VG709_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HWC31986.1"
                     /translation="MERRLLDILEFEGTWWRDDGTKADAISGRLGMSAARYYRLLDRV
                     IDMPEALRHDPLLVKRLRRRRTERRRQRLAAVYGLRI"
     gene            1822..2028
                     /locus_tag="VG709_02545"
     CDS             1822..2028
                     /locus_tag="VG709_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31987.1"
                     /translation="MAGKHEPESSSSFYGSLARTMATGTLRILLVAAALAIGVFVLAN
                     VFPDGAATSRILSVPVAMVLASDP"
BASE COUNT          326 a          687 c          739 g          297 t
ORIGIN      
        1 gcacccgcag gcgccgaagg aatgtcatcg accgcccacc ttagcgggcg gtgccgcgga
       61 ggggcgtgcg gcgcggtgcc gcacccgccc cgccgggtcg ctcaggatcg cgaacgtgag
      121 ggcgcggtca ccgatgtgga cgcaaactgt cgttcttgaa cgcctctccc actgcggctc
      181 gtccacacgg gtccctatcc cgctcgctgt agactccggt gcggacgcgg acgaaggagt
      241 cggcgtgaac gggaacgcct ggcaacaggc gctggagcag ctcgaccaag cagcccgcat
      301 catgaatctc gatccgaacg tgcacgaggt gctccgctac ccgaagcgca cgctcgaggt
      361 ctcgatcccc gtccgcatgg acgacgggtc ggtccgcgtc ttcaagggat accgcgtcca
      421 ccacaacacc gcgaggggcc cgtcgaaggg cgggatccgt taccacccga gcgtgcacct
      481 cgacgaggtg aaagcgctcg cgatgtggat gacgtggaag tgcgcggtcg tggggatccc
      541 gtacggcggc gcgaagggcg gcgtcgtggt caaccccaag cagctctcca cgcgtgagag
      601 cgagcgcctc acccgccgct acgcgtcgga gatcctgccg ctcatcggcc ccgagcgcga
      661 catcccggcg cccgacgtcg gcacggacga gcaggtgatg gcctggatca tggacacgta
      721 ctcgatgaac caggggttct cggtgcccgg ggtcgtcacc ggcaagcctc tttcggtcgg
      781 cgggtcggcc ggtcggcggg cgtcgaccgc gcgcggcgtg atgtacatca cggtcgcgac
      841 gctcaagcac ctcgggatgc cggtcgacga ggctcgcgtc gtggtccagg gctacgggaa
      901 ggtcggacgg ccggttgtcg agctcctcgc ggaccagggg tgccggatcg tcggcgtcgg
      961 cgacgtcgcg ggcggggtct acaacccccg cgggctctcg ccggtgggcc ttgaggcgca
     1021 tcacgaacag gccggcacgg tcgccgggtt cgacgggggc gagcggctga ccggcgagga
     1081 gctgctcgag gcggaatgcg atgtcctgat cccggccgcg ctcgagcggg tcgtcacgga
     1141 cgagaacgcc gatcgcgtga aggcccgcgt catcgtcgag gcggcgaacg gcccggtcac
     1201 cccggaggcc gacgagatct tccacgacgc aggggtccac gtcgtgcccg acgtcctcgc
     1261 gaacgccggc ggtgtcacgg tgtcgtactt cgagtgggtc caggacctgc aggcctactt
     1321 ctggtccgag gacgaggtga acgcgaggct tcgcgccgtc atggaggacg cctacatcga
     1381 cgtgctggat ctcgccgagg aacgtaaggt cacgatgcgc caggcagcga cgatcctcgg
     1441 cgtgcaacgc gtcgcggagg cgcaccgcac acgcggcctc tacccgtagc ggttccttga
     1501 atcacacgag cgcgctccct atcctcgggc tgcggcccca cgagacggaa ggacgagcgt
     1561 cccgtggagc gacgtctgct cgacatcctg gaattcgagg gcacgtggtg gcgcgacgac
     1621 ggaacgaagg ccgacgctat cagcggcagg ctcgggatgt cggccgcgcg ctactatcgg
     1681 ctgctggatc gggtcatcga catgccggag gcactccggc acgacccgct gctcgtgaag
     1741 aggcttcgcc gccgccgcac cgagcggagg cggcagcggc tcgccgcggt ctacgggctg
     1801 cgcatctgac ccggaggagg ggtggccggc aagcatgaac ccgagtcgag ctcgagcttc
     1861 tacgggtcgc tcgcgaggac catggcgacc ggcacgctga ggatcctgct ggtcgcggcg
     1921 gcgctcgcga tcggcgtgtt cgtgctcgcc aatgtcttcc ccgacggcgc cgcgaccagc
     1981 aggatcctca gcgtgccggt cgccatggtc ctcgcgagcg acccgtagaa gctcgagctc
     2041 gactcgggt
//
LOCUS       DASZOB010000154         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_3486,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000154 DASZOB010000000
VERSION     DASZOB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3486"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1471
                     /locus_tag="VG709_02550"
     CDS             491..1471
                     /locus_tag="VG709_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31988.1"
                     /translation="MSSRLRPLSERIVARLPGPYALWVFVWALVPWLNAGANLLLEDE
                     ARSAVWEQSRLLVILNYCALTVAIVITLWGTSRIARRLETLRAAGSTGIDDASRQPFR
                     EMNSVRGPLLACAVTAIAFGISGAVANGWASGILRGATWFVLGIPIWSFLWTYGSLQL
                     GLDRLGRQHLPPDAARVDPTLGLRPLGGAAFMGLWMLLAWLVPLVLTGLPDVVGALIG
                     IGVLLAALGMFTFSLRRLHRQMVDAKAAELAVARELYAQAYEPVRAAPSLETLERQRA
                     LLGAADALEKRAHAIHEWPIDEGTLARVLTITTSVIAMVIARMLLDPLGL"
     gene            1491..>2613
                     /locus_tag="VG709_02555"
     CDS             1491..>2613
                     /locus_tag="VG709_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWC31989.1"
                     /translation="MTTPAHEFPTDAGGLPDARRPEVVELSDGDEFELRIAPVAKQLG
                     QTTIRMLAYNGSVPGPTLRVRQGSDVAVTAVNEGDLEATVHWHGLRLDNRYDGTTETQ
                     APIPVGGTFTYRITFPDPGVYWYHPHIREDYGQEMGLYGNILVDPLDDILLEDGKVAP
                     FARDETTYTAMGRFGNVLLVNGEAELALNARRGEVVRLYLTDTANTRVFNVAVPGARM
                     KRVGGDSGHYEHEEFVEGVIIAPSERVAVDVLFEQAGELTLEHRTPDRTYPLAAIGVA
                     DEPAEPSFAAEFDELRSHADMATERERAAAFLDAEPDKTLALIAEMDIGEPEPPPGAG
                     VAYACPMHPDVIADEPGKCPKFGMKLLATAVAPIDYACPM"
BASE COUNT          392 a          890 c          891 g          440 t
ORIGIN      
        1 gtcgacgagc ccgatccgca ggatgccggg gacgtagccg taccgccgtc gcgccgcgag
       61 aacggcaagt ctccgcattc cgcgtgcctc gtcgggcgag accgtcttga tgcgtgccac
      121 cgggacctcc ctcgagccgc tgttggctgg aagagaatct tgtcacgccc cgacgatggg
      181 atgtcgaacc gccacgccaa tgctcgcgcg cggggcccgt gccggtcgcc gctcgaactt
      241 ttggctcaac ttttgcctgc gccagccgat gtgacgcgat cgcagcgcgt ctcccatgac
      301 gccagcgcgc ggaatgttgc ccagaggacg gcccgtggat gggctgttaa ccggacggtt
      361 gttggttcga gtccaacccg gggaccctcc gcgaggcccg cgcgctgcgg gcttttctgc
      421 gagcaggcgt cgtccgaggc gtttcttgcc acttcttccg cgacgcttct attcgcgatc
      481 atctggtggt gtgagcagcc ggcttcgacc actgagcgag cgaatcgtcg cgcgcctacc
      541 cggcccgtac gccctctggg ttttcgtgtg ggcgctcgtt ccgtggctca acgcgggtgc
      601 gaaccttctg ctcgaggacg aagcgcgaag cgcggtctgg gagcagagcc ggcttcttgt
      661 gatcctcaac tactgcgcgt tgacggtggc gatcgtgata acgctctggg ggacaagtcg
      721 gattgcgcga cgcttggaga cgcttcgcgc ggccggctcg acggggatcg acgacgcgtc
      781 gcggcagccc tttcgcgaga tgaacagcgt tcgcggcccg ctcctggcgt gcgccgtgac
      841 ggcgatcgcc ttcgggatca gcggtgccgt cgccaatggc tgggcgtccg gaatcctccg
      901 cggcgcaacc tggttcgtcc tgggaattcc gatctggtcg ttcctctgga catatgggtc
      961 gctgcagctc ggtctcgatc ggctcgggcg gcagcatctc cctcccgacg ccgctcgtgt
     1021 cgatccgacg ctcgggctgc gaccgctcgg cggtgccgcg ttcatgggcc tttggatgct
     1081 cctggcgtgg ctcgtccccc tcgtgctgac tggactgccg gacgtggtcg gcgccttgat
     1141 cggaatcggc gtactcctcg ccgcgctcgg aatgttcacc ttctcgctgc ggcgtctgca
     1201 caggcagatg gtcgacgcca aggcggccga gctggcagtc gcccgagagc tctacgcgca
     1261 ggcgtacgag ccggttcgcg cggcgccgtc gctggagacg ctcgaacgac agcgcgccct
     1321 tctcggtgcc gccgacgcgc tcgaaaagcg cgctcacgcg atccacgagt ggccgatcga
     1381 cgaaggaacg ctcgcccgcg tgctcacgat cacgacgagt gtcatcgcga tggtcatcgc
     1441 gcgtatgctc ctcgacccgc ttggcctttg accgctatct ggaggtcgcg atgacgacgc
     1501 ccgcgcacga gttcccgacc gacgccggcg gacttcccga cgcacgtcgg ccggaggtcg
     1561 tcgagctttc cgacggcgac gaattcgagc ttcggatcgc acccgtcgcg aagcagctcg
     1621 gccagacgac gatccgcatg ctcgcgtaca acggatcggt cccggggccg acgctgcgcg
     1681 tacgccaggg atccgacgtt gccgtcacgg cggtcaacga gggtgacctc gaggcgacgg
     1741 tgcactggca cggactgcgg ctcgacaacc gctacgacgg gacgacggag acgcaggcgc
     1801 cgatcccggt cggggggacg ttcacctacc ggatcacctt cccggacccc ggcgtctact
     1861 ggtatcaccc gcacatccgc gaggactacg gccaggagat ggggctgtac ggcaacatcc
     1921 tggtcgatcc gctcgacgac atcctgctcg aggacggcaa ggtcgcgccc ttcgcgcgcg
     1981 acgagacgac ctacaccgcg atggggcggt tcggaaacgt cctgctcgtg aacggggagg
     2041 ccgagctcgc actcaacgcg cgtcgcggcg aggtcgtacg cctctacctg accgacaccg
     2101 cgaacacgcg cgtcttcaac gtcgccgtgc cgggcgcgcg gatgaagcgt gttggcggcg
     2161 acagcggaca ttacgagcac gaggagttcg tcgagggcgt gatcatcgcc ccgtccgagc
     2221 gcgtcgccgt cgacgtcctc ttcgaacagg cgggcgagct gacgctcgag caccggacgc
     2281 cggatcggac gtatccgctc gcggcgatcg gcgtcgctga cgagccggcg gagccttcct
     2341 tcgcggcgga gttcgacgag ctccgaagcc acgccgacat ggcgaccgag cgcgagcggg
     2401 ccgccgcgtt tctcgacgcc gagccggaca agacgcttgc gctcatcgcc gagatggaca
     2461 tcggcgagcc ggagccgccg ccgggggcgg gggtcgccta tgcctgtccg atgcatcccg
     2521 acgtgatcgc ggacgagccc gggaagtgtc cgaagttcgg gatgaagctc ctcgcaacgg
     2581 cggtcgcccc gatcgactac gcctgcccga tgc
//
LOCUS       DASZOB010000155         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173326, whole genome shotgun sequence.
ACCESSION   DASZOB010000155 DASZOB010000000
VERSION     DASZOB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173326"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VG709_02560"
     CDS             <1..188
                     /locus_tag="VG709_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31990.1"
                     /translation="PTILGSGIDAVGDLGVSRVGEGINLVNRSLHVVGEDVVLAADVA
                     AAASDATGTEAGDSAPT"
     gene            complement(194..>2209)
                     /gene="polA"
                     /locus_tag="VG709_02565"
     CDS             complement(194..>2209)
                     /gene="polA"
                     /locus_tag="VG709_02565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWC31991.1"
                     /translation="LRARERGMEAVVAHADELKPRISAAVADAAEQIPRNKDLARLET
                     DLDLDVDPDSCVMGEWDPDEVRRLFHALEFRTLLERLEEIGRTRPLAESADLELSETS
                     AAGVRDILEGGEPVAVSVVADPGRVTGIGVSAGEARASFVPVAGTLPRPMEAWLRDAR
                     RPKWVHDAKELQTAALRTGTSVEGVAFDTMLAAYLLDPAEASYPLDGIAAKYLGTNLL
                     AGVDEGSGDGQLPLEAAWRRPAAEAAAVALLAPTLRERAERAGVLRLLEDVELPLARV
                     LAGMQAAGVALDVGYLSEMAESVGDRMTDLEAQIYSHAGEEFNLGSPQQLAHILYEKL
                     GLPVLKRTTKGRAPSTDADVLEKLRDHHPIIDVMLRYRELSKLKSTYLDALPPLVDAS
                     TGRIHTTFNQVAAATGRLSSVNPNLQNIPVRGELGRQIRKAFVARPDRLLLVADYSQI
                     ELRVLAHLSGDEALADAFASDADIHTATAAKVFALPPEQVDPELRRRAKVVNYGLAYG
                     MNAYGLASRLGIPPDEAQEFIDAYFDGFPKVREFLDRQVAVAAAEGFTATMLGRRRYL
                     PELQSANPRVRDLGRRMALNAPIQGSAADIIKLAMVAVDRAIEPLPATMVLTVHDELV
                     FEADASDLDDVSKVVREEMESAYELTVPLRADLGRGSNWAEAAPAGH"
BASE COUNT          300 a          785 c          786 g          338 t
ORIGIN      
        1 cccccacgat cctcgggtcg ggtatcgacg cggtcggtga cctcggcgtc tcgcgggtcg
       61 gagagggcat caatctcgtg aaccgctcgc tgcacgtcgt cggcgaggac gtcgtcctcg
      121 cagcggacgt ggccgcggcg gcctccgacg cgaccgggac ggaggccgga gactccgctc
      181 ctacgtagtc cgctcagtgc ccggccggtg cggcctcggc ccagttcgag ccgcgtccga
      241 gatcggcccg gagcggcacc gtgagctcgt acgcggactc catctcctcg cgcacgacct
      301 tcgacacgtc gtccaggtcg gacgcgtcgg cctcgaacac gagctcgtcg tggaccgtca
      361 gtaccatcgt cgccgggagg ggctcgatcg cgcggtcgac cgcgaccatg gcgagcttga
      421 tgatgtcggc cgcggatccc tggatcggcg cgttcaacgc catccgccgt cccaggtctc
      481 ggacccgcgg gttcgccgac tggagctccg gcaggtaccg acggcggccg agcatcgttg
      541 ccgtgaaccc ctcggccgcg gcgacggcga cctgccggtc gaggaactcg cgtaccttcg
      601 ggaacccgtc gaagtacgcg tcgatgaact cctgtgcctc gtccggcggg atgccgagcc
      661 ggctcgcgag cccgtaggcg ttcatgccgt acgcgagccc gtagttgacg acctttgcgc
      721 gccgccgcag ctcgggatcg acctgctccg gcggcagtgc gaagaccttc gcggcggtgg
      781 cggtatggat gtcggcgtcg gaggcgaacg catcggcgag cgcctcgtcg cccgacaggt
      841 gagcaaggac ccgtagctcg atctgcgagt agtccgcgac gagcaggagg cggtccggcc
      901 tcgcgacgaa cgccttgcgg atctggcggc cgagctcgcc gcggaccggg atgttctgca
      961 ggttcgggtt caccgacgac agccgccccg tggcggcggc gacctggttg aacgtcgtgt
     1021 ggatccttcc ggtgctcgcg tcgacgagcg gcggcagtgc gtcgaggtac gtcgacttga
     1081 gcttcgagag ctcgcggtac cgcagcatca cgtcgatgat cgggtgatgg tcccgcagct
     1141 tctcgagcac gtcggcgtcg gtcgacggcg ctcgtccctt cgtcgttcgt ttcagaaccg
     1201 gaagcccgag cttctcgtag aggatgtgtg cgagctgctg cggagagccg aggttgaact
     1261 cctcgcccgc gtgagagtag atctgcgcct ccaggtccgt catccggtcg ccgacgctct
     1321 ccgccatctc actgaggtag ccgacgtcga gcgcgacgcc ggcggcctgc atccccgcga
     1381 gcacgcgggc gaggggcagc tccacgtcct cgaggaggcg aaggacgccc gcgcgctcgg
     1441 cgcgctctcg cagcgtcggc gcgaggagtg ccacggccgc tgcctccgcc gccggacgtc
     1501 tccacgccgc ctcgagcggc agctgtccgt cgccggagcc ctcgtcgacg cccgcgagga
     1561 ggttggtgcc gaggtacttc gccgcgatcc cgtcgagcgg gtacgacgcc tccgccggat
     1621 cgagcaggta ggcggccagc atcgtgtcga acgcgacgcc ctcgaccgaa gtgccggtgc
     1681 ggagcgccgc ggtctggagc tccttcgcgt cgtgtaccca cttcggccgc cgggcgtcac
     1741 ggagccaggc ctccatcggt cgcgggagcg tcccggccac cgggacgaac gacgctctcg
     1801 cctcgcccgc cgagacaccg atcccggtaa cgcgaccggg gtcggcaacg acgctcacgg
     1861 cgaccggctc gccgccttcg aggatgtcgc ggacgccggc ggcgctcgtc tcgctcagct
     1921 cgaggtccgc ggactcggcg agcgggcgcg tccgcccgat ctcctcgagc cgctcgagca
     1981 gcgtgcggaa ctccagcgcg tggaaaaggc ggcggacctc atcggggtcc cactccccca
     2041 tgacgcacga gtccgggtcg acgtcgagat cgagatcggt ctcgagccgc gcgaggtcct
     2101 tgttgcgcgg gatctgctcg gccgcgtcgg cgacggcggc gctgatccga ggcttcagct
     2161 cgtcggcgtg cgcgacgacg gcctccatcc cccgctcacg ggcgcggag
//
LOCUS       DASZOB010000156         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84228, whole genome shotgun sequence.
ACCESSION   DASZOB010000156 DASZOB010000000
VERSION     DASZOB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84228"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VG709_02570"
     CDS             complement(<1..379)
                     /locus_tag="VG709_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31992.1"
                     /translation="MPAGTFGGRRRVGGHLAARFSTVDSGAWLRRVVAVAVALFVLLA
                     LAPTPGMAGGSGARDVCTTGDAQAAAVDLFGAGSKLVEIERISGLDTALVLEPRIYPG
                     TRRRMIAADGQWCEATSGFNPAWR"
     gene            complement(484..1155)
                     /locus_tag="VG709_02575"
     CDS             complement(484..1155)
                     /locus_tag="VG709_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31993.1"
                     /translation="MTALRSLVSMAIALGLVAVGPPAGASQLDARDRPTATGPLSYEG
                     RRCTSVDRLRIRSELVAVGRSCIFLYRFSPTAEVDVVRDYGVAWVQTQLDPRNGWCAT
                     SVRSRVTLPDDTEGHARSTTRYTTSKPRTIRPRLVADAGGAALSEGSLRQRMKLHPRR
                     MRGLLSAERRTFTVTWSGRTARPLAFALGLEFSAQAAEALLGDVGAFGGGLTGPLGFV
                     KRRGC"
     gene            1223..>1688
                     /locus_tag="VG709_02580"
     CDS             1223..>1688
                     /locus_tag="VG709_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWC31994.1"
                     /translation="MKRHPIFARFYEWLSQKVDVLEREYRVELASGARGRVLELGAGN
                     GLNFEHYRDASLVVALEPEPGMLRHARPRAAAAPVPVRRVQGAGERLPFPDASFDTVV
                     VSLVLCSVRDARRVADELRRVLRPRGEMRLFEHVRARDPKAARWQDRFATPWS"
BASE COUNT          236 a          598 c          627 g          227 t
ORIGIN      
        1 ggcgccaagc ggggttgaag ccggaggtcg cctcgcacca ctgcccatcg gcggcgatca
       61 tccggcggcg cgtccccgga tagatcctcg gctcgaggac gagcgcggtg tcgagcccgg
      121 agatccgttc gatctcgacc agcttggaac cggccccgaa caggtcgacg gcagccgctt
      181 gggcgtcgcc ggtcgtgcag acgtcgcgcg cgccggagcc gccggccata ccaggcgtcg
      241 gcgccaaggc gagcagaacg aagagcgcga ccgctactgc gacgacgcgc cgcagccacg
      301 cgcccgaatc gacggtcgag aagcgagctg caagatggcc gccgacgcgt cgtcggccgc
      361 cgaaggttcc cgcgggcatt aggatccctc cctgaggtgg tcgtccgcct ggttcgggcg
      421 ggttcttaca cattcgccgt cagaggcgag tttcctgcgg agccgtctga gaacgccggt
      481 cgatcagcag ccgcggcgct ttacgaagcc gagcggaccc gtgagcccgc cgccgaacgc
      541 gccgacatcg ccgaggagcg cctcggcggc ctgagccgag aactcgagcc cgagcgcgaa
      601 cgcgagcggg cgagccgttc gtccgctcca ggtcaccgtg aacgtccggc gttcggcgct
      661 gagaagcccg cgcatgcggc gcgggtggag cttcatccgt tgccggagcg agccctcgga
      721 gagcgctgcg ccgcccgcgt ccgcgacgag acgcgggcgg atcgtccgag gcttggaggt
      781 cgtgtacctc gtcgtgctgc gcgcgtgtcc ctcggtgtcg tccgggagcg tgacgcgcga
      841 gcggacggag gtcgcgcacc acccgttgcg cgggtcgagc tgcgtctgga cccatgccac
      901 gccgtagtcg cggacgacgt ccacctcggc cgtcggagag aagcggtaga ggaagatgca
      961 tgaccgtccg acggccacga gctcgctgcg gatgcgtagc cggtccacgg acgtgcagcg
     1021 ccgtccttcg tagctgagcg gtcccgtggc cgtcggccga tcgcgggcgt cgagctgcga
     1081 ggcgcccgcc gggggcccca ccgcgacgag gccgagcgcg atcgccatcg agacgaggga
     1141 gcggagcgcg gtcacgagcg acacgttcga cgggccgggt ccgattcctg ttccgtgcac
     1201 cgcggcgggc gacaatgacg ccgtgaagcg ccatccgatc ttcgctcggt tctacgagtg
     1261 gctctcgcag aaggtcgacg tgctcgagcg cgagtaccgc gtcgagctcg cgagcggcgc
     1321 gcgcggccgc gtcctcgagc tcggggccgg caacggcctc aacttcgagc actaccgcga
     1381 cgcgtcgctc gtcgtggcgc tggagccgga gccggggatg ctgcgccacg cccggccgag
     1441 ggcggccgcc gccccggtcc ccgtccggcg cgtgcagggc gccggcgagc ggctgccgtt
     1501 ccccgacgcc tcgttcgaca cggtcgtggt gagcctcgtg ctctgttcgg tccgcgacgc
     1561 tcgccgcgtc gccgacgagc ttcgccgggt cctccggcca cggggagaga tgcggctctt
     1621 cgagcacgtc cgcgcccgcg accccaaggc ggcacggtgg caggaccgct tcgcgacgcc
     1681 gtggagct
//
LOCUS       DASZOB010000157         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_132953, whole genome shotgun sequence.
ACCESSION   DASZOB010000157 DASZOB010000000
VERSION     DASZOB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132953"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>1703
                     /gene="pnp"
                     /locus_tag="VG709_02585"
     CDS             27..>1703
                     /gene="pnp"
                     /locus_tag="VG709_02585"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWC31995.1"
                     /translation="MTDEVRPQGHRVSVEIGGTEITIETGRLAKQASGSVVVRAGDTV
                     VLGTATAGEPSDGDFLPLTVDVEERMYAAGRIPGSFFRREGRAGERGTLTARMIDRPL
                     RPLFPKGWRRETQLVSMPVSVDQVNSYDVLAMNGASAALMVSEIPFPAPVGAVRIGKD
                     EEGNFVVNPDEATLLDPELDLVVSGTEEAILMVEAGAKEISEAEILDALDIAHGEIRK
                     LCQAQRELADKAGRPKSEVEVPQVDESLVEQINQQFGSAMQAATQVEDKLERQEATKQ
                     VEADVHAAFSERPPDAAGDPDRRQAVQLAFDKLEKDAVRHRIAVEKKRPDGRVADEVR
                     PIWIEVGALPRAHGSAIFTRGQTQALSVAALGTTREELRVDDLGVRDEKRYFHHYNFP
                     PLSVGEAGFMRGPKRRDIGHGALAERALVPVVPSQEAFPYTLRVVSDILESNGSSSMA
                     SVCGSSLSLMDAGVPIARPVAGIAMGLIKEGEDYTVLSDIAGVEDHLGDMDFKVAGTE
                     RGITALQMDIKIADVTFEILRDALDQALAGRRHILAKMAEAIDEPRAELSE"
BASE COUNT          279 a          593 c          604 g          227 t
ORIGIN      
        1 aaccggaggc ggcgcgaaag gaacgcatga cagacgaagt acgcccgcaa gggcaccgcg
       61 tctcggtcga gatcggcggc acggagatca cgatcgagac cggacggctc gctaagcagg
      121 cgagcgggtc cgtggtcgtc cgcgccggcg acaccgtggt cctggggacc gcgacggccg
      181 gcgagccgag cgacggcgac ttcctcccgc tcaccgtgga cgtcgaggag cgcatgtacg
      241 ccgcgggcag gatccccggc tcgttcttcc gccgcgaggg ccgcgccgga gagcggggca
      301 cgctcaccgc gcgcatgatc gaccgcccgc tgcgtccgct ctttcccaag gggtggcggc
      361 gcgagaccca gctcgtgtcc atgccggtct cggtcgacca ggtcaattcc tacgacgtgc
      421 tcgccatgaa cggcgcgagc gcggcgctca tggtctccga gatcccgttc ccggcgcccg
      481 tcggagcggt gcggatcggc aaggacgagg agggcaactt cgtcgtcaac cccgacgagg
      541 cgacgctgct cgaccccgag ctcgacctcg tggtctccgg caccgaggag gcgatcctca
      601 tggtcgaggc gggcgccaag gagatctccg aggcggagat cctcgacgcc ctcgacatcg
      661 cccacggcga gatacgcaag ctctgccagg ctcagcgcga gctggccgac aaggcgggca
      721 ggccgaagag cgaggtcgag gtgccgcagg tagacgagtc gctggtcgag cagatcaacc
      781 agcagttcgg ctcggccatg caggccgcga cccaggtgga ggacaagctc gagcgccagg
      841 aggccaccaa gcaggtcgag gccgacgtgc acgctgcctt ctccgagcgg ccgcccgacg
      901 cggccggcga ccccgatcgc cgtcaggccg tccagctcgc cttcgacaag ctcgagaagg
      961 acgccgtccg ccatcggatc gccgtcgaga agaagcggcc cgacggacgc gtcgccgacg
     1021 aggtccgtcc gatctggatc gaagtcggtg ccttgccgcg cgcgcacggg tcggcgatct
     1081 tcacccgcgg tcaaacccag gcgctctcgg tggccgcgtt ggggaccacg cgcgaggagc
     1141 tgcgcgtcga cgacctcggc gtccgcgacg agaagcgcta cttccaccac tacaacttcc
     1201 cgccgctctc ggtcggggaa gcgggcttca tgcgcgggcc caagcgccgc gacatcggtc
     1261 acggcgcgct cgccgagcgg gcgctcgtcc cggtcgtgcc ctcgcaggag gcgtttccct
     1321 acacgctgcg cgtggtctcc gacatcctcg agtccaacgg gtcgtcctcg atggcctcgg
     1381 tgtgtgggtc gagcctctcg ctgatggacg cgggcgtgcc gatcgcgcgg ccggtggcgg
     1441 gcatcgccat ggggctgatc aaggagggcg aggactacac cgtcctgtcc gacatcgccg
     1501 gcgtcgagga ccacctcggc gacatggact tcaaggttgc cggcaccgag cgggggatca
     1561 ccgcgctcca gatggacatc aagatcgcgg acgtcacctt cgagatcctg cgggatgcgc
     1621 tcgaccaggc cctggcgggc cgcaggcaca tcctggccaa gatggccgag gcgatcgacg
     1681 agccgcgcgc cgagctctcg gag
//
LOCUS       DASZOB010000158         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_3556,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000158 DASZOB010000000
VERSION     DASZOB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VG709_02590"
     CDS             <1..412
                     /locus_tag="VG709_02590"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HWC31996.1"
                     /translation="YEPENHEWATWVRAAKVQAVAEEIPPIEIDDPSGDADLLVIGWG
                     STYGAIRVGVERLRAKGRKVARAHLYHMNPLPKNTAEVLRQYRKILIPEMNLGQLWRI
                     LRAEFLAPAELYSKVQGQPIFAEEIEAEVERRLG"
     gene            409..1473
                     /locus_tag="VG709_02595"
     CDS             409..1473
                     /locus_tag="VG709_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWC31997.1"
                     /translation="MTDGNGHQGNGQGNGQPVAVQLTKKDFTSDQEPRWCPGCGDYAI
                     LSAVQAFLPELEVPPHKMVWVAGIGCSGRFTYYVDTYGVHGIHGRAPALATGIATARP
                     DLSVWVATGDGDALSIGGNHLIHAVRRNVDIKILLFNNQIYGLTKGQYSPTSPEGKVT
                     KSTPFGSLDHPFNPVALALGAEAAFVARTIDNDRQHLTETLRAAANHRGAAFIEIYQN
                     CNVFNDGAFAAVRDKQHGARENQIRLEHGKPIRFGATGERGVVQRPDGSLATVDVADV
                     GEEALLVHDAHRAEPSLAFALAHLSEAPTGPTPIGVFRDVQRPVYGEAMERQLEAAKE
                     KLGEGDLAKLLHSGDTWVVE"
     gene            1509..2312
                     /locus_tag="VG709_02600"
     CDS             1509..2312
                     /locus_tag="VG709_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWC31998.1"
                     /translation="MLTLNLWGRRGSWEDRRRVLVDGLRDVRPDLVAFQEAIVTADYD
                     QPLDLLGDGFHFAHQKNRDPDGQGVSIASRWPIDGVDELDLHVTPRTADFACTTLVAD
                     VRSPVARMLFANHFPSWQPRFEAERERQAVIAARFLEERVEDDRPHVVVAGDLDADPD
                     AASIRFWTGRLSLEGMSVCYRDAWESAHHGEPGQTFSPTNPLVEDHDWPFRRIDYILV
                     RCGLHGGPTLAIVGCERVFDEPSAGVWASDHFGVRADLALPGDRSRKSL"
     gene            2405..2587
                     /locus_tag="VG709_02605"
     CDS             2405..2587
                     /locus_tag="VG709_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC31999.1"
                     /translation="MATKETGDKPREQKAPQGKPEAHVCSVCGTKLSSYNPGPNCWQH
                     TIGYPWRGPSAKPRFD"
     gene            complement(2588..>3693)
                     /locus_tag="VG709_02610"
     CDS             complement(2588..>3693)
                     /locus_tag="VG709_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC32000.1"
                     /translation="SDLSRVETEARLENGAWRLYGEKWFCSNVDAGLILTIARTDPDP
                     GAVGLSVFLVPAEVGAERNRFRIERLKEKLGVASMPTGEIVYDGAEGYLVGERGQGVR
                     VAAELFNLSRIYNAVASVAGAKRALREAVAWTREREAFGAPVARQPLAMQDLARLTVE
                     LEANLALVFETARALDAVDAGAGDHERRLLRILTPVAKLITAKFAVRATSEAMELLGG
                     NGYIEDFDTPRLLRDAQVLPIWEGTTNVLVLDAVRSMMKQGTLETLLEDARRRLPEGG
                     EMAVEAKQVREALDRAESDAGPVLSGGLAGARHVVERLVTAYRSSLLVEAADRGGDRD
                     RVVAARYVEGFLTPAMEGGPLDLSDGYDELVGL"
BASE COUNT          580 a         1322 c         1288 g          503 t
ORIGIN      
        1 gtacgagccc gagaaccacg agtgggcgac atgggtgcgg gcggcgaagg tccaggccgt
       61 cgcggaggag atccccccga tcgagatcga cgacccgagc ggtgacgccg atctcctcgt
      121 gatcgggtgg ggctcgacgt acggcgcgat ccgggtgggg gtcgagcggc tccgcgcgaa
      181 gggccggaag gtcgcgcgcg cgcacctcta ccacatgaac ccgctgccga agaacacggc
      241 ggaggtgctc cggcagtacc ggaagatcct gatccccgag atgaacctcg gccagctctg
      301 gcggatcctg cgtgcggagt tcctcgcgcc ggcggagctg tacagcaagg ttcagggcca
      361 gccgatcttc gccgaggaga tcgaggccga ggtcgagagg cggctcggat gaccgacggc
      421 aacggacacc agggcaacgg gcaggggaac ggccagccgg tcgcggtcca gcttacgaag
      481 aaggacttca cgagcgacca ggagccgcgc tggtgccccg gttgcggtga ctacgcgatc
      541 ctgtcggccg tccaggcgtt cctgccggag ctcgaggtgc cgccgcacaa gatggtgtgg
      601 gtcgcgggca tcgggtgctc cggacggttc acgtattacg tcgacacgta cggcgtccac
      661 ggcatccacg gccgggcgcc ggcgctcgca accggcatcg cgacggctcg accggatctc
      721 tcggtctggg tcgcgacggg cgacggcgac gcgctctcga tcggcgggaa ccacctgatc
      781 cacgccgtgc ggcggaacgt cgacatcaag atcctgctgt tcaacaacca gatctacggg
      841 ctgacgaagg ggcagtactc gccgacgagc cccgagggca aggtgacgaa gtcgacgccg
      901 ttcggctcgc tcgaccaccc gttcaatccg gtcgcgctcg cgctcggggc cgaggcggcg
      961 ttcgtcgccc gcacgatcga caacgaccgc cagcacctca cggagacgct ccgcgcggcg
     1021 gcgaaccacc gcggcgcggc gttcatcgag atctatcaga actgcaacgt gttcaacgac
     1081 ggcgcgttcg cggccgtccg cgacaagcag cacggcgcgc gcgagaacca gatccgcctc
     1141 gagcacggca agccgatccg cttcggcgcg acgggggagc ggggcgtcgt ccagcgtccc
     1201 gacgggagcc tcgcgaccgt cgacgtcgcc gacgtgggcg aggaggcgct gctcgtccac
     1261 gacgcgcacc gcgccgagcc gtcactcgca ttcgcgctcg cgcacctgtc cgaggcgccg
     1321 accggcccga ccccgatcgg cgtgttccgc gacgtgcagc ggccggtgta cggcgaggcg
     1381 atggagcgcc agctcgaggc cgccaaggag aagctcgggg agggcgacct cgccaagctc
     1441 ctccactccg gcgacacgtg ggtcgtcgag taaccggcga caccccggac gcagtcgatc
     1501 gagtccgcgt gctcacgctc aacctctggg ggcgccgcgg ctcctgggag gaccgccgac
     1561 gcgtgctcgt cgacgggttg cgcgacgtcc ggcccgatct cgtcgcgttc caggaagcca
     1621 tcgtcacggc tgactacgac cagccgctcg atctcctcgg cgacgggttc cacttcgcgc
     1681 accagaagaa ccgggacccc gacggacagg gcgtctcgat cgccagccgc tggccgatcg
     1741 acggcgtcga cgagctcgat ctccacgtca ccccacgaac cgcggacttc gcgtgcacga
     1801 cgctggtcgc ggacgtgcgt tcgcccgtcg cgcgcatgct gttcgccaac cacttcccga
     1861 gctggcagcc tcgcttcgag gcggagaggg agcgacaggc cgtgatcgcc gcgcgcttcc
     1921 tcgaggagcg cgtcgaagac gaccggccgc acgtcgtcgt tgccggcgac ctcgacgccg
     1981 atccggacgc cgcgagcatc cggttctgga ccggacgcct gtcgctcgag ggcatgagcg
     2041 tctgctaccg cgacgcgtgg gagagcgcgc atcacggcga gccgggccag accttctcgc
     2101 cgacgaaccc gctcgtggag gatcacgact ggccgttccg ccggatcgac tacatcctcg
     2161 tgcgctgcgg gctccacggc ggccccacgc tcgcgatcgt cgggtgcgag cgggtcttcg
     2221 acgagccgtc cgcgggcgtc tgggcgagcg atcacttcgg ggtgcgcgcc gacctcgcgt
     2281 tgcccggcga ccgtagccgg aaatcgctgt gaccagcgga aacgcggctc ggctttacac
     2341 cgccggtatg attatgtcgc ctgaccgggg gtcagagcgg agcccgcaac ccatcgggag
     2401 agccatggcg accaaggaga ccggcgacaa gccccgcgag cagaaggcgc cgcaggggaa
     2461 gcccgaggct catgtgtgct ccgtctgcgg caccaagctg tcgtcgtaca atcccggccc
     2521 caactgctgg cagcacacga tcgggtaccc gtggcggggg ccctccgcga aaccgcgctt
     2581 cgactgacta gagcccgacg agctcgtcgt agccgtcgct gagatcgagc ggcccgccct
     2641 ccatcgccgg cgtgaggaac ccctcgacgt agcgcgccgc tacgacgcgg tcgcggtcgc
     2701 cgccccggtc cgccgcctcg acgagcagcg acgagcggta tgcggtgacg agccgctcga
     2761 cgacgtggcg agcgccggcg agcccccccg atagcaccgg gccggcgtcc gactccgccc
     2821 gatcgagcgc ctcacggacc tgcttcgcct cgaccgccat ctccccgcct tcaggaaggc
     2881 ggcgccgggc gtcctcgagc agggtctcga gcgtcccctg cttcatcatc gagcggacgg
     2941 catcgagcac gaggacgttc gtcgtcccct cccagatcgg caggacctgc gcgtcgcgga
     3001 gcaggcgtgg cgtgtcgaag tcctcgatgt acccgttgcc gccgaggagc tccatcgcct
     3061 cgctcgtggc gcgaaccgcg aacttcgcgg tgatgagctt ggcaaccggc gtgaggatgc
     3121 gaagcagccg gcgctcgtgg tcgccggcgc cggcgtcgac ggcatcgagc gcgcgtgccg
     3181 tctcgaagac cagggcgagg ttcgcctcga gctcgaccgt gagccgcgct aggtcctgca
     3241 tcgcgagcgg ttggcgcgcc accggcgccc cgaaggcctc ccgttctcgc gtccaggcga
     3301 cggcctcgcg gagcgcgcgc ttcgcgccgg cgaccgacgc gaccgcgttg tagatccgcg
     3361 agaggttgaa gagctcggcg gcgacccgga cgccctggcc ccgctcgccg acgaggtacc
     3421 cctcggcgcc gtcgtagacg atctcgcccg tcggcatcga cgccacgccg agcttctcct
     3481 tgagccgctc gatccggaag cggttgcgct ccgcgccgac ctcggccggc acgaggaaca
     3541 ccgacagccc caccgcgccg ggatcggggt ccgtcctcgc gatcgtgagg atgaggccgg
     3601 cgtcgacgtt ggagcagaac catttctccc cgtacaggcg ccacgcgccg ttctcaagtc
     3661 tggcctcggt ctcgacgcgg gagaggtccg acc
//
LOCUS       DASZOB010000159         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84256, whole genome shotgun sequence.
ACCESSION   DASZOB010000159 DASZOB010000000
VERSION     DASZOB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84256"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /gene="acs"
                     /locus_tag="VG709_02615"
     CDS             complement(<1..1718)
                     /gene="acs"
                     /locus_tag="VG709_02615"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWC32001.1"
                     /translation="MRTEAPAGHAIDALFLEERRYEPPDDFAAQANARPDIYERDFEE
                     FWETEGRERVTWFEPFEKLYEWEPPYAKWYLGGKLNVCFNCVDRHVETGSGEKVAYHW
                     EGEPVGERRTVTFADLQRDVVRFANGLKKLGVRKGTPVAIYMGMVPELPVAMLACARL
                     GAPHTVVFGGFSADALADRMNDMECEVLITQDEGWRKGNRVPLKRNADDALATSPGVR
                     HAVVLRRTEGEVAMEQARDVSWDELCADVSDDPESCPCEPMDSEDLLYLLYTSGTTAK
                     PKGIVHTTAGYLVGVATTHHYIFDVKPDSIYWCAADIGWVTGHSYIVYGPLCNGTTSV
                     LYEGTPDYPDKDRWWEIVERYEVDILYTAPTAIRAHMKWGPEHAEKHDLSSLRLLGSV
                     GEPINPEAWEWYRRHIGGGRTPVVDTWWQTETGMIMISPLPGVTTLKPGSATRPFPGV
                     DAAVYDEQGNEVGPGGGGYLVLRRPWPAMLRGLYKEHDRYVETYWTGPAKEASADVDA
                     YEDVYFTGDGARIDEDGDFWLLGRVDDVMNVSGHRISTIEVESALVDHPRVAEAAVCG
                     RNDALTG"
     gene            complement(1715..>1942)
                     /locus_tag="VG709_02620"
     CDS             complement(1715..>1942)
                     /locus_tag="VG709_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32002.1"
                     /translation="MYMTLYQDMEHWFGISPEAIGSVGAAINFAVTIVVSLLTREPSA
                     EIQALVEEVRHPRIGAAPETVPAPAPAGGGK"
BASE COUNT          269 a          700 c          641 g          332 t
ORIGIN      
        1 cctgtgagcg cgtcgttgcg gccgcagacg gcagcctcgg cgacgcgcgg gtggtcgacg
       61 agcgcgctct cgacctcgat cgtcgagatc cggtgcccgg agacgttcat gacgtcgtcg
      121 actcggccga ggagccagaa gtcgccgtcc tcgtcgattc gtgccccgtc gcccgtgaag
      181 tagacgtcct cgtaggcgtc cacgtcggcg ctcgcctcct tcgccggtcc cgtccagtac
      241 gtctcgacgt agcgatcgtg ctccttgtag aggccgcgga gcattgccgg ccacggccgc
      301 ctgaggacga ggtacccgcc tccgcccggc ccaacctcgt tcccctgctc gtcgtagacc
      361 gcggcgtcga cgccggggaa cggcctggtc gccgagccag gcttgagcgt cgtgaccccg
      421 gggagcggcg agatcatgat catcccggtc tcggtctgcc accacgtgtc gaccacgggc
      481 gtgcgtccgc cgccgatgtg ccgccggtac cactcccacg cctccgggtt gatcggctcg
      541 ccgaccgagc cgagcaggcg gagcgacgac aggtcgtgct tctccgcgtg ctcggggccc
      601 cacttcatgt gcgcgcggat cgcggtcggc gccgtgtaca ggatgtccac ctcgtagcgc
      661 tcgacgatct cccaccagcg gtccttgtcg gggtagtccg gcgtgccctc gtagagaacg
      721 ctcgtcgtcc cgttgcagag ggggccgtag acgatgtagc tgtggccggt gacccagccg
      781 atgtcggccg cgcaccagta gatcgagtcg ggcttgacgt cgaagatgta gtggtgggtc
      841 gtcgcgacgc cgacgaggta gccggccgtc gtatgcacga tccccttcgg cttcgcggtc
      901 gtcccgctcg tgtagagcag gtagaggagg tcctccgagt ccatcggctc gcacgggcac
      961 gactcggggt cgtccgagac gtccgcgcag agctcgtccc acgacacgtc tcgcgcctgc
     1021 tccatagcca cttcgccttc ggtcctccgc aacacgaccg cgtgacgcac gccgggcgaa
     1081 gtagcgagcg cgtcgtccgc gttccgcttc agagggacgc ggttcccctt ccgccaccct
     1141 tcgtcctgcg tgatcagcac ctcgcactcc atgtcgttca tccggtctgc gagggcgtcg
     1201 gcgctgaacc cgccgaagac gacggtgtgc ggagccccga gccgcgcgca cgcgagcatc
     1261 gcgaccggta gctccgggac catgcccatg tagatcgcga ccggcgtgcc cttccggacg
     1321 cccagcttct tgagcccgtt cgcgaagcgg acgacgtcgc gctggaggtc ggcgaacgtg
     1381 accgtccggc gctcgccgac gggctcgccc tcccagtggt acgcgacctt ctcgcccgag
     1441 cccgtctcca cgtgccggtc gacgcagttg aagcagacgt tcagcttgcc gccgaggtac
     1501 cacttcgcgt acggcggctc ccactcgtag agcttctcga acggctcgaa ccacgtgacg
     1561 cgctcgcggc cctccgtctc ccagaactcc tcgaagtccc gctcgtagat gtcgggcctc
     1621 gcgttcgcct gcgccgcgaa gtcgtcaggt ggctcgtagc gccgctcctc gaggaagagg
     1681 gcgtcgatcg cgtggcccgc gggcgcctcg gttctcattt cccacctccg gcgggggcgg
     1741 gtgccgggac cgtctccggc gccgcgccga tgcgcggatg gcgcacctct tccacgagcg
     1801 cctggatctc ggccgacggc tcccgcgtca gcagggacac cacgatggta accgcgaagt
     1861 tgatcgcagc gccgacggag ccgatcgcct cgggcgagat cccgaaccag tgctccatgt
     1921 cttggtagag cgtcatgtac at
//
LOCUS       DASZOB010000160         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_184433, whole genome shotgun sequence.
ACCESSION   DASZOB010000160 DASZOB010000000
VERSION     DASZOB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184433"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VG709_02625"
     CDS             complement(<1..201)
                     /locus_tag="VG709_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32003.1"
                     /translation="MTAAGRSMGHSLRNILLLVALCALAVSASACGGGDDDARTPEDV
                     PPDAIALVQDEKIPRSEFDELME"
     gene            complement(198..>1569)
                     /locus_tag="VG709_02630"
     CDS             complement(198..>1569)
                     /locus_tag="VG709_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWC32004.1"
                     /translation="VEMVSRFRRPSDVKRILRDFGAGKVDVLVGTHRVLSRDVAAKDL
                     GLVVLDEEQRFGVAQKELLRQLRLEVDVLALTATPIPRTLHMSLSGLRDISVINTPPE
                     GRRPIKTYVGEYDEEVVKQALLREHARKGQAFFLHNRVETIDEKALSLAQLCPELRFL
                     VVHGQLPERELEERMLSFLAGDADVLVTTTIIESGLDIPQANTLIVERADALGLAQLY
                     QIRGRVGRSDVTAHAYLFYLDGQELTSEARARLAALADHTELGAGFAIAMRDLEIRGA
                     GNLLGAEQSGHVAAIGFELYLELLDEAVAELTGARRPVTRPVRVEAQVDAFVPASYVD
                     AEAQKIDLHRRLALVESEDELRELHAAVEDRYGPVPEPVENLFGIQEAKLKLARLGAD
                     YLVVRAGKATVGPLVLGSAELRELRRTAGTAVYSSARRELSRRGDGLPPAIDLVDAIL
                     GLRLAA"
BASE COUNT          247 a          546 c          553 g          223 t
ORIGIN      
        1 ctccatgagc tcgtcgaact ccgagcgcgg gatcttctcg tcctgaacga gggcgatcgc
       61 atccggcggt acgtcctccg gcgtcctcgc gtcgtcgtct ccgccgccgc aggcggacgc
      121 ggacacggcg agcgcgcaga gcgccacgag cagcaagatg tttcgaagag agtgacccat
      181 cgaacgaccg gctgccgtca cgcggcgagc cgtagcccga ggatagcatc gacgaggtca
      241 atcgccggag gcaagccgtc accccgccgg gaaagctcgc gccgcgccga ggagtagacc
      301 gcggtaccgg ccgtccggcg gagctcgcga agctcggcgg agccgagcac gagggggccg
      361 accgtcgcct tcccggcgcg aacgacgagg tagtccgctc cgagtcgcgc gagcttgagc
      421 ttcgcctcct ggatcccgaa caagttctcg accggctcgg gaacggggcc gtagcgatcc
      481 tccacggccg cgtgcagctc gcgcagctcg tcttccgact cgacgagggc gagccggcgg
      541 tggaggtcga tcttctgcgc ctcggcgtcg acgtacgacg ccgggacgaa ggcgtcgacc
      601 tgggcctcga cgcggacggg ccgcgtcacg gggcgccggg cgccggtcag ctcggcgacc
      661 gcctcgtcga gaagctcgag gtagagctcg aacccgatcg ccgcgacgtg gccggactgc
      721 tcggcgccga gaaggttgcc cgcgccgcgt atctcgaggt cgcgcatcgc gatcgcgaag
      781 ccggcgccca gctccgtatg gtccgcgagc gcggccagcc tggcgcgggc ctccgacgtg
      841 agctcctgcc cgtcgaggta gaagaggtag gcgtgcgccg tgacgtcgct ccgcccgacg
      901 cgaccgcgga tctggtacag ctgggcgagc ccgagcgcgt cggcgcgctc gacgatcaac
      961 gtgttcgcct ggggaatgtc gaggccggac tcgatgatcg tcgtcgtgac gagcacgtcg
     1021 gcgtcgcccg cgaggaacga gagcatccgc tcctcgagct cgcgctctgg cagctgaccg
     1081 tggacgacga ggaagcgaag ctcgggacag agctgcgcaa gcgatagcgc tttctcgtcg
     1141 atcgtctcga ccctgttgtg caggaagaac gcctgaccct tgcgcgcgtg ctcgcgcagc
     1201 agcgcctgct tgacgacctc ctcgtcgtac tcgccgacgt aggtcttgat cggccgccgg
     1261 ccctcgggcg gcgtgttgat gaccgagatg tcccgcaggc ccgagagcga catgtggagc
     1321 gtgcgcggaa tcggcgtcgc cgtcagcgcg aggacgtcga cctcgaggcg cagctgacgc
     1381 agcagctcct tctgcgcgac cccgaagcgc tgctcctcgt ccaggacgac gagcccgagg
     1441 tccttcgccg caacgtcgcg cgagagaacg cggtgcgtgc cgacgaggac gtcgaccttc
     1501 cccgcgccga agtcgcgcag gatccgcttg acgtccgacg gcctccggaa tctcgacacc
     1561 atctcgacg
//
LOCUS       DASZOB010000161         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_124280, whole genome shotgun sequence.
ACCESSION   DASZOB010000161 DASZOB010000000
VERSION     DASZOB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124280"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VG709_02635"
     CDS             <1..283
                     /locus_tag="VG709_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWC32005.1"
                     /translation="RLADLPGIRLYGPPAERRGGIVSFDLEGVHPHDVAQILGWEGIA
                     VRAGHHCTQPLMRRLGTPATTRASFYLYTLTEEIDRLVEGLHRVKRTFA"
     gene            367..891
                     /locus_tag="VG709_02640"
     CDS             367..891
                     /locus_tag="VG709_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32006.1"
                     /translation="MDGRVYVVDPVDAEGVDERLRARGEPAAVLVLLDRHRRDADAFA
                     ERLAVPVHETPFAGVPETPFEVRPVVRRRVWREVVLWWPERRVLVTADALGTGGYMTV
                     GDEPLGVHPFLRLTPPRTAVAGLEPEHVVVGHGEGIHGPAAARALAAALAGSRRRILP
                     GLGEALRKPLERLR"
     gene            925..1356
                     /locus_tag="VG709_02645"
     CDS             925..1356
                     /locus_tag="VG709_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWC32007.1"
                     /translation="MGYDQMYREVILDHYKNPRGHGVIDDPDAEAEGQNPLCGDEVTI
                     FVAFGADGETVEDVKFRGRGCAISQAATSMLVEMVKGRTAEDIASMPRDELLDEVGIP
                     LTPVRLKCALLGLGVLKVALNRAKGTALPAEWAGMDELEIS"
     gene            1409..>1639
                     /locus_tag="VG709_02650"
     CDS             1409..>1639
                     /locus_tag="VG709_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWC32008.1"
                     /translation="MAELTVCPLDELPAGTMKLVHAGEISVGVYNVGGELYAIEDRCS
                     HDDGPLVEGDWEPDEAIVVCPSHGATFASRTGK"
BASE COUNT          219 a          587 c          603 g          230 t
ORIGIN      
        1 acgactcgcc gacctgccgg ggatccgcct ctacgggccg cccgccgagc gccggggcgg
       61 gatcgtgtcc ttcgacctcg aaggcgtgca cccgcacgac gtcgcccaga tcctcggctg
      121 ggaaggcatt gccgttcggg cgggtcacca ctgcacgcag ccgctcatgc gccgcctcgg
      181 cacgccggcc acgacgcgcg cgagcttcta cctctacacg ctcacggagg agatcgaccg
      241 gctggtcgag ggcctccaca gggtcaagcg gacgttcgcc tgacccgctt ctgcgatgag
      301 acggacttcg ggttcggctg gctcgacccg gacgacgcgc tcaggcggac gtcgcacgcg
      361 ctcgcggtgg acgggcgcgt ctacgtcgtc gaccccgtcg acgccgaggg ggtcgacgag
      421 cgcctccgcg cgcgcggtga gccggcggcg gtgctcgtcc tgctcgatcg gcaccgacgg
      481 gacgcggacg ccttcgcgga gcgactcgcc gtcccggtgc acgagacacc gttcgccggc
      541 gtgccggaga cgccgttcga ggtgcgcccg gtcgtgcggc gccgcgtctg gcgcgaggtg
      601 gtgctctggt ggcccgagcg ccgcgtcctc gtcactgccg acgcgctcgg aaccggcggg
      661 tacatgaccg tgggcgacga gccgctcggc gtccatccct tcctgcgcct cactccgccg
      721 cggacggccg tggcgggcct cgagccggaa cacgtcgtcg tcggccacgg agagggcatc
      781 cacggtcctg cggccgcgcg ggcgctcgcg gccgcgctcg cgggctcgcg gcgccggatc
      841 ctccctgggc tcggcgaggc gctgcgcaag ccgctcgagc gcctgcgctg aagtcggcct
      901 cggccacgcg gctacgatgg ccgggtggga tacgaccaga tgtaccgcga ggtgatcctc
      961 gaccactaca agaatccgcg cggccacggc gtcatcgacg atccggacgc ggaggccgag
     1021 ggccagaatc cgctctgcgg cgacgaggtc acgatcttcg tcgccttcgg ggccgacggc
     1081 gagacggtgg aggacgtgaa gttccgcggt cgcggctgcg cgatcagcca ggcggcgacg
     1141 tcgatgctgg tcgagatggt caagggccgc acggccgagg acatcgcgtc catgccgcgc
     1201 gacgagctgc tcgacgaggt cggcatccca ctcacgcccg tgaggctcaa gtgcgccctg
     1261 ctcggcctcg gcgtgttgaa ggtcgcgctc aatcgggcga aggggacggc gcttccggcc
     1321 gagtgggccg gcatggacga gctcgagatc tcgtgaggcc tcggcgcctt ccctgcggcg
     1381 gcgcaggcgc cgcggcttcg cgcgcctgat ggccgagctg acggtgtgtc ccctcgacga
     1441 gctccccgcc ggcacgatga agctcgttca cgccggcgag atctccgtgg gcgtctacaa
     1501 cgtcggcggt gagctctacg ccatcgagga ccgctgctcg cacgacgacg gcccgctcgt
     1561 cgaaggcgac tgggagcccg acgaggcgat cgtcgtctgc cctagccacg gggcgacgtt
     1621 cgccagccgt acggggaag
//
LOCUS       DASZOB010000162         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173365, whole genome shotgun sequence.
ACCESSION   DASZOB010000162 DASZOB010000000
VERSION     DASZOB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173365"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..>1584)
                     /gene="betA"
                     /locus_tag="VG709_02655"
     CDS             complement(2..>1584)
                     /gene="betA"
                     /locus_tag="VG709_02655"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HWC32009.1"
                     /translation="ADPATRVLVLEAGRTDWRWDVFIHMPAALAFPIGSRFYDWKYES
                     EPEPFMGGRRVYHARGKVLGGSSSINGMIFQRGNPLDYERWAEEPGLESWSYLHCLPY
                     LKRMENCLAARPDDPYRGHEGPLVLERGPATGPLFRAFFEAVQQAGHALTEDVNGYKQ
                     EGFAPFDRNIHRGRRLSASRAYLHPVMKRPNIEVRTRAFVSRVVFEGTRAVGVEVGGE
                     TVHAGEVILCGGAINSPQLLLLSGVGNAAELGALGVETVHDLPGVGENLQDHLEVYVQ
                     HGSKQPVSVAPAYRWRSRPWVGARWLFLRAGPGATNHFEGGGFIRSNDDVAYPNLMFH
                     FLPIAIRYDGSQPAGGHGYQVHVGPMTSDARGSVKLKSRDPAVHPALRFNYLSTEQDR
                     QEWVEVVRHARATLGQPAFDDFSAGELSPGPSVATDEEILAWVARDAETALHPSCTCR
                     MGTDELAVVDPATMCVRGLDGLRVVDASVFPFVPNGNIYAPVMMVAEKACDLLLGNTP
                     LEPEQVDFYRHRREAVSA"
BASE COUNT          234 a          558 c          568 g          224 t
ORIGIN      
        1 ctcatgccga gaccgcctcg cgccggtggc ggtagaagtc gacctgctcg ggctcgagcg
       61 gcgtgttgcc gaggaggagg tcgcatgcct tctcggcgac catcatcacc ggcgcgtaga
      121 tgttcccgtt ggggacgaag ggaaagaccg acgcgtcgac cacgcggagg ccgtcgaggc
      181 cgcgcacgca catggtggcc ggatcgacga ccgcgagctc gtcggtcccc atgcgacacg
      241 tgcacgaagg gtgcagcgcg gtctccgcgt cgcgcgcgac ccacgcgagg atctcctcgt
      301 cggtcgcgac tgacggcccg ggggagagct cccccgcgct gaagtcgtcg aacgccggct
      361 ggccgagggt cgcccgcgcg tggcggacca cctcgaccca ctcctgccgg tcctgctcgg
      421 tggagaggta gttgaaccga agcgcggggt gcaccgccgg gtcgcgcgac ttcagcttca
      481 cggagccgcg cgcgtcggag gtcatcggtc cgacgtggac ctggtagccg tggccgcccg
      541 ccggctgaga gccgtcgtag cggatcgcga tcgggaggaa gtggaacatc aggttcgggt
      601 acgcgacgtc gtcgttcgag cggatgaacc cgcccccctc gaagtggttg gtagcgccgg
      661 gacccgctcg taggaagagc caccgcgccc cgacccacgg ccggctgcgc cacctgtacg
      721 cgggcgcgac cgagaccggc tgcttcgatc cgtgctggac gtacacctcg aggtggtcct
      781 gcaggttctc gccgacgccg gggagatcgt gcaccgtctc gaccccgagg gcgccgagct
      841 ccgccgcgtt cccgacgccg gagaggagca ggagctgcgg cgagttgatc gcgccgccgc
      901 agaggatgac ctcgccggcg tgcaccgtct ccccgccgac ctcgacgccc acggcgcgcg
      961 tcccctcgaa gaccacgcgt gacacgaacg cgcgcgtcct cacctcgatg ttcgggcgtt
     1021 tcatgacggg gtgcaggtac gcgcgcgagg cgctcagccg gcggccgcgg tggatgttgc
     1081 ggtcgaaggg cgcgaagccc tcctgcttgt agccgttcac gtcctcggtc agggcatgcc
     1141 ccgcctgctg gactgcctcg aagaacgcgc ggaagagggg cccggtcgcg ggaccgcgct
     1201 cgagcacgag cggcccctcg tgcccgcggt acgggtcgtc gggccgagcg gcgaggcagt
     1261 tctccatccg cttgaggtac gggaggcagt ggaggtagct ccaggactcg agccccggct
     1321 cctccgccca gcgctcgtag tcgagcgggt tcccccgctg gaagatcatc ccgttgatcg
     1381 agctcgagcc gcccagcacc ttgccgcgcg cgtggtacac gcgccgcccg cccatgaacg
     1441 gctccggctc cgactcgtac ttccagtcgt agaagcggct cccgatcggg aacgcgagcg
     1501 cggccggcat gtggatgaag acgtcccagc gccaatcggt ccgaccggcc tccagcacga
     1561 gcacgcgcgt agcgggatcg gcgc
//
LOCUS       DASZOB010000163         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_124307, whole genome shotgun sequence.
ACCESSION   DASZOB010000163 DASZOB010000000
VERSION     DASZOB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124307"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VG709_02660"
     CDS             complement(<1..261)
                     /locus_tag="VG709_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWC32010.1"
                     /translation="MRTEPLHRAVGLTDEEYERVVATRGRDPNRAELAMYGAMWSEHC
                     SYKSSKVHLRGLPTEGEHVLVGPGEDAGVVDVGDGVACVFKME"
     gene            complement(258..962)
                     /gene="purQ"
                     /locus_tag="VG709_02665"
     CDS             complement(258..962)
                     /gene="purQ"
                     /locus_tag="VG709_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWC32011.1"
                     /translation="MSARVAVVTFPGSLDDRDAMRAVRLMGGEPVPVWHGDRDLRGAD
                     AVILPGGFSYGDYLRTGAIARFARVMDAVRKHAERGGPVLGICNGFQILSEAGLLPGA
                     LIRNRTLSFICRRIHVRCESAATPFTHGVDPAQALEIPIKHGEGQFVADAETLERLEG
                     DGRVVFRYCSPAGEVGDAWNPNGAANAIAGIANERGNIVGLMPHPEHAVDPDVGPTGG
                     RAIFESLLTSVLAAAR"
     gene            complement(959..1237)
                     /gene="purS"
                     /locus_tag="VG709_02670"
     CDS             complement(959..1237)
                     /gene="purS"
                     /locus_tag="VG709_02670"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWC32012.1"
                     /translation="MTKQFQVVVMLNEGLADPQGKAIEDALPTLGFEGVSEVRVGKVI
                     HLTMEGPTDEEIRRRVHEISERVLSNPVIESFWIVDVEEGSDVEVAAP"
     gene            complement(1234..2112)
                     /locus_tag="VG709_02675"
     CDS             complement(1234..2112)
                     /locus_tag="VG709_02675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWC32013.1"
                     /translation="MQAQGKVRDIYDVGDDRLLMVTSDRISAFDVVLPNPIPDKGRVL
                     TGLSLFWFERTKDIVPNHLLTADVDGFPERFAEHRDELAGRSMLVRRAQMVPIECVAR
                     GYLSGSGWKEYRSTGRVCGIELPSGLTESDRLPDPIFTPATKAETGHDENITIERAAE
                     IVGEGLVQRLKELTLEIYELGADLARERGVILADTKFEFGFIDGEIALCDEVLTPDSS
                     RFWPADDYRPGGAQPSFDKQFVRDWLDTSGWNHEPPPPDLPADVIEQTASRYREAYER
                     VTGESFDQYLRRSGIR"
     gene            complement(2127..3431)
                     /gene="purB"
                     /locus_tag="VG709_02680"
     CDS             complement(2127..3431)
                     /gene="purB"
                     /locus_tag="VG709_02680"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWC32014.1"
                     /translation="MIPRYTLPEMADVWSEEAKLRHWLRIEILACEAWAKLGRIPEED
                     LAAIRDRAAVTADRVAEIEKATRHDVAAFVQAAGESVGPAGRWIHFGMTSSDLLDTTL
                     ALQLREAADLLLRRVERVLAAVRSRALEHRDTVCIGRTHGVHAEPTTFGHKLAVWAFE
                     LARDRDRLRRAKDVVSVGKVSGAVGTYAAVDPFVEEYVCAELGLRPADAATQIVQRDR
                     HAEYMSALSITGATLEKIAVEIRHLARTEVREVEEPFEIGQRGSSAMPHKRNPVLCER
                     ITGLARVLRGNLQAALENVALWHERDISHSSVERIVFPDSTLALDYMLHLADFVVREM
                     RVFRERMRENVNIGGGVVFSQPVLLALIDRGMTREQAYHLVQAAAAAAWDRGADFRAE
                     LAKTKSVRDILPSDELAALFEPRLEHLDAVFSRLEKLEVNGS"
     gene            complement(3439..3912)
                     /gene="purE"
                     /locus_tag="VG709_02685"
     CDS             complement(3439..3912)
                     /gene="purE"
                     /locus_tag="VG709_02685"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWC32015.1"
                     /translation="MAERGPVVGILVGSASDLPVLQQGAAILERLEIPHEIQVMSAHR
                     TPDLVDEYARTAEDRGLVTVICGAGMAAHLAGAVAARTVLPVIGVPVSSDYLGGADAL
                     YSTVQMPRGVPVATVAINGAVNAALLAAQMIAVRDPEVRKRLHEFKESLAEGLKL"
     gene            complement(3905..>4218)
                     /locus_tag="VG709_02690"
     CDS             complement(3905..>4218)
                     /locus_tag="VG709_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HWC32016.1"
                     /translation="LGWTGDASVTVVLASGGYPGPYEIGFPISGLEAAEDLDGVTVFH
                     AGTARRDGKVVTAGGRVLAVSALGKDLAAARERAYDAAGRISFEGKHVRSDIALEASR
                     G"
BASE COUNT          633 a         1534 c         1432 g          619 t
ORIGIN      
        1 ctccatcttg aagacgcacg cgactccatc tccgacgtcg acgacgcccg cgtcctcccc
       61 cggcccgacg aggacgtgct ctccctcggt cgggagaccc cgaagatgca ccttgctcga
      121 cttgtacgaa cagtgctccg accacatcgc gccgtacatc gcgagctcgg cgcggttcgg
      181 gtcgcgtccg cgcgtcgcga cgacgcgctc gtactcctcg tcggtgagcc cgacggcgcg
      241 gtggagcggc tcggtcctca ccgcgcggcg gcgaggaccg acgtgagcag cgactcgaag
      301 atcgcgcggc cgccggtcgg gccgacgtcg ggatcgacgg cgtgctccgg gtgcggcatc
      361 agccccacga tgttcccgcg ctcgttcgcg atcccggcga tcgcgttggc ggcgccgttc
      421 gggttccagg cgtcgccgac ctcaccggcc ggcgagcagt agcggaacac gacgcgcccg
      481 tcgccctcga ggcgctcgag ggtctcggcg tccgcgacga actggccctc gccgtgcttg
      541 atcgggatct cgagggcctg cgcggggtcg acgccgtgcg tgaacggcgt ggccgccgac
      601 tcgcaccgga cgtggatccg tcggcagatg aacgacagcg tgcggttgcg aatcagcgca
      661 cccggcagca ggccggcctc ggacaggatc tggaacccgt tgcagatccc gagcacgggg
      721 ccgccgcgct cggcgtgctt ccgtacggcg tccatgacgc gagcgaagcg cgcgatcgcg
      781 ccggtccgga gatagtcgcc gtacgagaag ccgccgggaa ggatcacggc gtcggcaccg
      841 cgcaggtcgc gatcgccgtg ccacacgggc accggctcgc cgcccatcag ccggaccgcg
      901 cgcatcgcgt cgcggtcgtc gagcgagccc ggaaacgtca cgacggcgac gcgggcgctc
      961 acggcgccgc gacctccacg tccgacccct cctcgacgtc gacgatccag aacgactcga
     1021 tgaccgggtt gctcaggacg cgctcggaga tctcatgaac gcgccgccgg atctcctcat
     1081 cggtcgggcc ctccatcgtg agatggatga ccttgccgac gcgaacctcg gagacaccct
     1141 cgaagccgag cgtggggagc gcgtcctcga tcgccttccc ctgcgggtcc gcgagcccct
     1201 cgttgagcat cacgacgacc tggaactgct tcgtcaccgg atgcccgacc tccgcaggta
     1261 ctggtcgaag gactcgccgg tgacgcgctc gtacgcctcg cggtaccggg aggcggtctg
     1321 ctcgatcacg tccgccggca gatcgggcgg cgggggctcg tggttccagc cgctcgtgtc
     1381 gagccagtcg cggacgaact gcttgtcgaa cgacggctgc gcgccgcccg gacggtagtc
     1441 gtccgcgggc cagaaccgcg acgagtccgg ggtcagcacc tcgtcgcaga gcgcgatctc
     1501 cccgtcgatg aacccgaact cgaacttcgt gtcggcgagg atgacgcctc gctctcgcgc
     1561 gagatccgcc cccagctcgt agatctcgag cgtgagctcc ttcaggcgct ggacgagccc
     1621 ctccccgacg atctcggccg cccgctcgat cgtgatgttc tcgtcgtggc cggtctccgc
     1681 cttcgtcgcc ggcgtgaaga tcgggtcggg aagccggtct gactcggtga gccccgacgg
     1741 aagctcgatc ccgcatacgc gtccggtcga gcggtactcc ttccacccgg atcccgacag
     1801 gtagccgcgc gcgacgcact cgatcggcac catctgcgcc ctacgcacga gcatcgaccg
     1861 cccggcaagc tcatcccggt gctccgcgaa ccgctccggg aacccgtcga cgtcggcagt
     1921 gaggagatgg ttcggcacga tgtccttcgt gcgctcgaac cagaagagcg acagcccggt
     1981 gagcacgcgg cccttgtcgg ggatcgggtt cgggagcacg acgtcgaacg cggagatacg
     2041 gtcgctcgtg accatgagca gccggtcgtc accgacgtcg tagatgtcgc ggaccttgcc
     2101 ctgcgcctgc atctcggcca gcacgctcac gacccgttca cctccagctt ctcgaggcgc
     2161 gagaacaccg cgtcgagatg ctcgagccgt ggctcgaaca gcgccgcgag ctcgtcggat
     2221 ggaaggatgt cccgcacgct cttcgtcttc gccagctcgg cgcggaagtc ggcgccgcgg
     2281 tcccacgcgg ccgcggcggc cgcctgcacg aggtggtagg cctgctcgcg cgtcatgccg
     2341 cgatcgatga gcgccagcag gacgggctgc gagaagacga ccccgccgcc gatgttcacg
     2401 ttctcgcgca tgcgctcgcg gaacacgcgc atctcgcgaa cgacgaagtc ggcgagatgc
     2461 agcatgtagt cgagggcgag cgtcgagtcc gggaacacga tgcgctccac agaagagtga
     2521 gagatgtcgc gctcgtgcca gagagcgacg ttctcgaggg ccgcttggag gttcccgcgc
     2581 agcacacgag ccaggccggt gatccgctcg cagagcaccg ggttccgctt gtggggcatc
     2641 gccgacgacc cgcgctgccc gatctcgaag ggctcctcga cctcgcggac ctccgtccgc
     2701 gcgaggtgcc tgatctcgac ggcgatcttc tcgagcgtcg cgccggtgat cgagagcgcg
     2761 ctcatgtact cggcgtgtcg atcccgctgg acgatctgag tggcggcatc cgccgggcgc
     2821 aacccgagct cggcgcagac gtactcctcg acgaacgggt cgaccgccgc gtacgtgccg
     2881 accgcgccgc tcaccttccc gaccgagacg acgtccttcg cgcgccggag ccggtcgcgg
     2941 tcgcgcgcga gctcgaacgc ccacacggcg agcttgtgcc caaacgtcgt cggctccgcg
     3001 tgcacgccgt gggtcctccc gatgcagacg gtgtcgcggt gctcgagcgc ccgcgacctc
     3061 actgccgcga ggacgcgctc gacccttcgc aggaggaggt cggccgcctc gcggagctgg
     3121 agcgcgagcg tggtgtcgag cagatcgctc gaggtcatcc cgaagtggat ccagcgtccg
     3181 gctggcccca cggactcgcc ggcggcctgg acgaacgcgg cgacgtcgtg gcgcgtcgcc
     3241 ttctcgatct ccgcgacgcg gtcggcggtc acggcggccc ggtcgcggat cgccgccagg
     3301 tcctcctcag ggatgcgtcc gagcttcgcc cacgcctcac acgcgaggat ctcgatccgg
     3361 agccagtggc gaagcttcgc ctcctccgac cacacgtcgg ccatctcggg gagcgtgtag
     3421 cgagggatca ctgtcgcctc acagcttcag gccctcggcg agggattcct tgaactcgtg
     3481 gaggcgcttc ctgacctccg ggtcgcgcac cgcgatcatc tgtgcggcga gcagcgccgc
     3541 gttcacggcg ccgttgatcg cgacggtcgc gacgggaacg ccgcgcggca tctgaaccgt
     3601 ggagtagagc gcgtcggcgc cgccgaggta gtccgacgag accgggacgc cgatcaccgg
     3661 cagcaccgtc ctcgcagcga cggcgcccgc gaggtgcgcg gccatcccgg cgccgcagat
     3721 caccgtcacg agcccgcggt cctccgcggt ccgcgcgtac tcgtcgacga gatcgggggt
     3781 ccggtgcgcc gacatcacct ggatctcgtg ggggatctcg aggcgctcga ggatcgcggc
     3841 gccctgctgg aggaccggaa ggtccgacgc cgagccgacg aggatgccga cgacgggtcc
     3901 acgctcagcc acgagatgcc tccagagcga tgtcgcttct cacgtgcttc ccctcgaacg
     3961 agatgcgccc cgcggcgtcg tacgcccgct ccctggcggc agcgaggtcc ttccccaagg
     4021 cgctgacggc caacacccga ccacctgcgg tcaccacttt accgtcccgt cgggccgtcc
     4081 ccgcgtggaa gaccgtgacg ccgtcgagat cctcggcggc ttccagcccg gagatcggaa
     4141 agccgatctc gtacggcccc ggatacccgc ccgaggcgag gacgaccgtg acggacgcgt
     4201 cgccggtcca cccgagcc
//
LOCUS       DASZOB010000164         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_144573, whole genome shotgun sequence.
ACCESSION   DASZOB010000164 DASZOB010000000
VERSION     DASZOB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144573"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..519)
                     /locus_tag="VG709_02695"
     CDS             complement(4..519)
                     /locus_tag="VG709_02695"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWC32017.1"
                     /translation="MHETADDLRALQRLIDESHRGGGRHLRTIYSDERHLSAHDLSQL
                     LRGVRVLNVATVTARCEPRVAPVDGLFFRGRWYFGSSPESVRFRHLRARLQVSATHVR
                     GEDVAVVVHGTATLVDPDASEHADFRGYLRATYPGWDSWARGNPYARIEASKMFTFGG
                     IQETSDHGDDG"
     gene            574..1083
                     /locus_tag="VG709_02700"
     CDS             574..1083
                     /locus_tag="VG709_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWC32018.1"
                     /translation="MGEARQFGRYFEDFEVGDVYRHWPGKTITEYDDHLFCAITWNHH
                     PLHTNEHYAETSTQFKRNVVVGNLVYSLALGMSVPDVSGKAIANLEVESLQHKAPFFH
                     GDTLYAATTVLDKKESSSKPDRGVVAVETRGYNQDGVEVLYFRRKVLVPKRGSVEEQP
                     GRPEPQASE"
     gene            1080..1478
                     /locus_tag="VG709_02705"
     CDS             1080..1478
                     /locus_tag="VG709_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32019.1"
                     /translation="MIATHVQRFSIDYGVWRPLLSVLGLGPKYSRVELMGDALHVRMG
                     WGFEARIPRSSIANPRRGRATLGIGAHTDLRGGWLVNGSLRGIVWMDVDPPARGRSMG
                     IRVRVRRLGVGVDDPHRLIAELAARGPARS"
     gene            1486..2169
                     /locus_tag="VG709_02710"
     CDS             1486..2169
                     /locus_tag="VG709_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWC32020.1"
                     /translation="MGRRRELARLADEVVRCTRCPRLVAWREAVSVDPPRRYAGQKYW
                     SRPLPGFGDPDARLLIVGLAPAAHGGNRTGRIFTGDRSADFLYVALHETGYANQPTSE
                     HPGDGLELRDAYVCAVNRCAPPDNKPTPRERDNCLPYLVRELELLDRLSVIVALGAFA
                     WDGVLRALAAHGHVERPRPKFGHGAEASVGPYRIVGAFHPSQQNTFTGRLTMEMLVDV
                     FTRAAELVS"
     gene            2166..2738
                     /locus_tag="VG709_02715"
     CDS             2166..2738
                     /locus_tag="VG709_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32021.1"
                     /translation="MTSHDDPGWGSALRASGPLLFVPFFPLLVLILRRKSLDALTALR
                     VLFLHYASALILILIVSAIIQPRLLSEDAPVPILVVLGAGSIGSLFGLWWSRGRPLDT
                     SNEQALAQSYRTLFFLSMAFAQSPALFGFVGVFVLDALWPYLVGLAISAYASSTFAPS
                     ARNLDRQQQQITASGSDLSLREAIQQQSSP"
     gene            2779..4716
                     /locus_tag="VG709_02720"
     CDS             2779..4716
                     /locus_tag="VG709_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32022.1"
                     /translation="MRGHYWSFEPDPDAGPIDRATVRRVAGTFRPYKRQVWLIALAIL
                     VTSSLGVVNPLLIKAVFDRALFVPGGPNLDLLYIFVGFMVLVPIVSGIIGIGQTYLSN
                     VVGQRVMQDLRNSVYAHLQRMPLRFFTSTRTGEIQSRLSNDIGGVQDVVTDTASSVLA
                     NVVIVLSTVVAMTIISWELTLLSLGTIPFFVYLTRRVGKARRQVASSTQQSMAELSAI
                     AEETLSVSGMLLSKAFGRRRYETERFRRENERLARLQTRQQMIGRSFFATIQIFFSIT
                     PALVYLVGGIVMAGGGGGLTAGDIVAFTTLQSRLFFPVGQMLQVGVEVQSAMALFDRV
                     FEYLDLPREIEDRPGAAALEPSAVRGRVALRNVWFHYEGPPDEAPVSPPDEAQRRDGQ
                     PRTWTLEDVDLEVKPGQLAALVGPSGAGKTTVTYLIPRFYDVQRGAVEIDGRDVRDIK
                     IDSLGEIVGMVTQETYLFHTTIRDNLRYGKPDASDEELVAASKAAFIHDRIAELPAGY
                     DTVVGERGYRMSGGEKQRLAIARVVLKDPRILILDEATSSLDTTSERLVQEALRPLMA
                     GRTTIAIAHRLSTILSADVIFVLDRGRVVERGTHAELIRRGGLYARLYEQQFKGGEVE
                     AECEDGVVLASGEVVGRGAGG"
BASE COUNT          721 a         1622 c         1664 g          790 t
ORIGIN      
        1 cgctcatccg tcgtctccgt ggtcggacgt ctcttggatc cccccgaacg tgaacatctt
       61 cgaggcctcg atgcgagcgt aggggttgcc tctcgcccag gagtcccatc ccgggtaggt
      121 cgctcggagg tacccgcgga agtcggcgtg ctcggatgca tccgggtcga cgagcgtggc
      181 cgtcccatgg acgacgacgg cgacgtcctc tccgcggacg tgcgtagcgc tcacctgtag
      241 tcgcgcccga aggtgacgaa agcggaccga ttccggtgac gagccgaagt accaccgtcc
      301 ccggaagaag aggccgtcca cgggagcgac gcgaggctcg caccgagcgg tgaccgtcgc
      361 gacgttgagg acgcggacgc cgcggaggag ctgcgagagg tcgtgcgcgc tcaggtgccg
      421 ttcatcgctg tagatggttc ggaggtgacg tcccccaccg cggtggcttt cgtcgatcag
      481 ccgctggagc gcccggaggt cgtccgcggt ctcgtgcatg gccggactca ggctagcatc
      541 gcgctgttcc gcgtcacccg atcaggagga agcatgggcg aggcgaggca gttcgggcgc
      601 tatttcgagg acttcgaggt cggcgacgtc taccggcact ggccagggaa gacgatcacc
      661 gagtacgacg accacctgtt ctgtgcgatc acgtggaacc atcatccgct ccacaccaac
      721 gagcactacg cggagacctc gacgcagttc aagcggaacg tggtcgtcgg caacctcgtg
      781 tattcgctgg cgctcggcat gagcgttccc gatgtgagcg ggaaggcaat cgcgaacctc
      841 gaggtcgagt cgctccagca caaggcgccg ttcttccacg gcgacacgct ctacgccgcg
      901 accacggttc tcgacaagaa ggagtcgtcg tcgaagcccg accgtggcgt cgtcgcggtc
      961 gagacccgcg gctacaacca ggacggcgtc gaggtcctgt acttccgccg gaaggtgctg
     1021 gtgccgaagc gggggagcgt cgaggaacag cccggccggc ccgagccgca ggcgtccgag
     1081 tgatcgccac gcacgtccag cggttctcga tcgactacgg cgtctggcgg ccgctgctct
     1141 cggtgctcgg gctcgggccg aagtactcga gggtggagct catgggcgac gccctccacg
     1201 tccggatggg gtgggggttc gaggcgagga tcccgcgctc gtcgatcgcg aacccgcgtc
     1261 gagggcgcgc gacgctcggc atcggcgcac acaccgatct ccgcggcgga tggctcgtca
     1321 acgggtcgct ccgaggcatc gtctggatgg acgtcgatcc gcccgcgcgc ggacggtcga
     1381 tgggcatccg cgtgcgggtc cgccgcctcg gcgtgggcgt agacgatccc caccgtctga
     1441 tcgcggaact ggcggcgcgg ggtccagcgc gttcgtaacg tcgacgtggg acgtcgccgc
     1501 gagcttgcgc gcctggcgga cgaggtcgtt cgctgcacga ggtgcccgcg gctggtcgcg
     1561 tggcgcgagg cggtgtcggt agatcctcct cgtcggtacg cgggacagaa gtactggtct
     1621 cggccgctgc cgggcttcgg cgatccggat gcgaggctgc tgatcgtcgg gctcgctccg
     1681 gcggcgcacg gagggaaccg aaccggacga atcttcaccg gcgaccgctc ggccgacttc
     1741 ctctacgtcg ccctacacga gaccggctac gcgaaccagc ccacgtccga gcacccggga
     1801 gacggtctcg agctgcgcga cgcctacgtc tgcgcggtga accggtgcgc gccgcccgac
     1861 aacaagccga cgccgcggga gcgcgacaac tgcctcccgt acctcgtgcg ggagctcgag
     1921 ctgctcgatc ggctgagcgt gatcgtcgcg ctcggcgcgt tcgcgtggga cggcgtgctg
     1981 cgcgccctcg cggcgcacgg ccacgtcgag agaccgcggc cgaagttcgg gcacggcgcc
     2041 gaggcgtcgg tcgggcccta caggatcgtc ggggcgttcc atccgagcca gcagaacacg
     2101 ttcacgggca ggctgacgat ggagatgctg gtcgacgtgt tcacgcgcgc cgcggagctg
     2161 gtttcatgac gagccacgat gatccaggtt ggggctctgc cctgagggcg agcggcccgc
     2221 tgctcttcgt tccgttcttc cctctgctcg tcctgatcct tcgacggaag tcgcttgacg
     2281 cgctgacggc gctgcgcgtg ctcttcctcc actacgccag cgcgctgatc ctcatcctca
     2341 tcgtgtcggc gatcatccag ccgcggctgc tttcggagga cgctccggtt ccgatcctgg
     2401 tcgttctcgg cgcgggctcc atcggcagcc tcttcggact ctggtggtca cgcgggcgac
     2461 cgctcgacac gtcgaacgag caggctctgg cacagtcgta ccgcacgctc ttcttcctga
     2521 gcatggcctt cgcacagtcg cccgcgctct tcggcttcgt cggagtcttc gtcctcgacg
     2581 ctctctggcc gtatctcgtg gggctggcga tcagcgcata cgcctcctcc acgttcgcgc
     2641 cgagcgcgcg gaacctcgat cgacagcagc aacagatcac ggccagcggg tcggacctct
     2701 ccctgcgcga ggcgatccag caacagtcga gcccgtagtg cccgaacgga aacgcgaccc
     2761 ggtcatatcc tggctgtcgt gcgcggtcac tactggagct tcgagccgga tcccgacgcg
     2821 gggccgatcg atcgcgcgac ggtccggcgc gtcgctggga cctttcggcc gtacaagcgc
     2881 caggtctggc tgatcgcgct cgcgattctc gtcacgtcga gcctcggcgt cgtgaacccg
     2941 ctgctgatca aggccgtgtt cgaccgggcg ctgttcgtgc ccggcgggcc gaacctcgac
     3001 ctgctctaca tcttcgtcgg gttcatggtc ctcgtcccga tcgtctccgg gatcatcggg
     3061 atcggccaga cgtacctatc gaacgtcgtg ggtcagcgag tgatgcagga cctgcggaac
     3121 tcggtgtacg cgcatctcca gcggatgccg ctgcgcttct tcacctcgac gcggaccggc
     3181 gagatccagt cgcggctctc gaacgacatc ggcggcgtcc aggacgtcgt gaccgacacc
     3241 gcctcgagcg tgctcgcgaa cgtcgtgatc gtgctcagca cggtcgtcgc gatgacgatc
     3301 atctcgtggg agctgacgct gctctcgctc gggacgatcc cgttcttcgt gtacctcacg
     3361 cgccgcgtcg ggaaggcacg ccggcaggtc gcctcgtcga cgcagcagag catggcggag
     3421 ctatccgcga tcgccgagga gacgctctct gtatcgggga tgctgctttc gaaggcgttc
     3481 gggcgccgac gctacgagac cgagcgcttc cggcgcgaga acgagcggct ggcgaggctc
     3541 cagaccaggc agcagatgat cgggcgctcg ttcttcgcga cgatccagat cttcttctcg
     3601 atcacgccgg cgctcgtcta cctcgtcggc gggatcgtga tggccggagg gggcggcggc
     3661 ctcacggccg gcgacatcgt cgcgttcacc acgctccaga gccggctgtt cttcccggtc
     3721 ggacagatgc tccaggtcgg cgtcgaggtc cagagcgcga tggcgctgtt cgaccgcgtc
     3781 ttcgagtacc tcgacctgcc gcgcgagatc gaggaccgcc ccggcgcggc cgcgctagag
     3841 ccctcggcgg tgcgcgggcg tgtcgcgctc cggaacgtgt ggttccacta cgaggggccg
     3901 cccgacgagg caccggtgag cccgcccgac gaggcgcagc gtcgcgatgg gcagccacgg
     3961 acgtggacgc tcgaggacgt cgacctcgag gtgaagcccg gccagctcgc cgcgctcgtc
     4021 gggccgagcg gagccgggaa gacgaccgtc acctacctga tcccgcgctt ctacgacgtc
     4081 cagcgcggcg ccgtcgagat cgacgggcgc gacgtgcgcg acatcaagat cgacagtctc
     4141 ggcgagatcg tcgggatggt cacgcaggag acgtacctct tccacacgac gatccgcgac
     4201 aacctgcggt acgggaagcc cgacgcctcc gacgaggagc ttgtggcggc gtcgaaggca
     4261 gcgttcatcc acgaccggat cgcggagctg ccggctggct acgacacggt cgtgggggag
     4321 cgcgggtacc ggatgtcggg cggcgagaag cagcggctcg cgatcgcgcg ggtcgtgctc
     4381 aaggacccgc ggatcctcat cctcgacgag gccacgtcgt cgctcgacac gacgtcggag
     4441 cggctggtgc aggaggcgct tcgtccgctg atggccgggc gcacgacgat cgcgatcgcc
     4501 caccgccttt cgacgatcct gtccgccgac gtgatcttcg tcctcgaccg aggtcgcgtc
     4561 gtcgagcgcg ggacccacgc cgagctgatc cgccgcggcg ggctctacgc gcgcctgtac
     4621 gagcagcagt tcaagggcgg cgaggtcgag gcggagtgcg aggacggcgt cgtgctcgcg
     4681 tccggcgagg tcgtcggacg aggcgccggc ggctagatcc ggagctcgta cacgcgcagc
     4741 cggaagtcat cgtcccagac cttgtcctcc cgccgtgcga accccatcga ctcatag
//
LOCUS       DASZOB010000165         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_74163, whole genome shotgun sequence.
ACCESSION   DASZOB010000165 DASZOB010000000
VERSION     DASZOB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74163"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1168
                     /locus_tag="VG709_02725"
     CDS             218..1168
                     /locus_tag="VG709_02725"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWC32023.1"
                     /translation="MVSAVPAAILLACLYLVDLPFYVVSPGPADDALPLIDIDGRETF
                     PSGGRLLFTTVYAGPGNVYDVARASVDDGARVVPESLFLQPGQTDAEYDAQQRSAMDQ
                     SKLDAAAVALREVTRYPERHGRGAVVESVFRGTPAEGRLFPGDLVISAGDRAVRSIDD
                     LADAIQRAGAGGELSVEVRPIEGGDVRRVVLRPARIGGDVVIGVSLIENFPFDVRIES
                     GDIGGPSAGLMWAIGIVDLLTPGDLTGGRTIAGTGDIDLEGNVAPIGGITEKLIAAER
                     AGAELFLVPEANMEEARATPASIDLVSVATLDDALHSLEG"
     gene            1165..1353
                     /locus_tag="VG709_02730"
     CDS             1165..1353
                     /locus_tag="VG709_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32024.1"
                     /translation="MNPTTETTLLFAAHMLALLVALGTALVLGGEVTRTRTARVTGSL
                     GFVSIAVAEAIHGAGLAA"
     gene            1372..>3444
                     /locus_tag="VG709_02735"
     CDS             1372..>3444
                     /locus_tag="VG709_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWC32025.1"
                     /translation="MLRAAGYLLLLFAAVMPIGMEGAGAFLALPAGAIVPASVGLVAG
                     AMSGWRRRGERGGVWLGAGVMLLAIGDSMLVVDRPWVLGLSHAVRSAGYLAVGWFAYA
                     ATRHSIRFRMVTGFVALLLAVVLAMSSAVAQVIATNLRESAIASVSQQGANAETEMVS
                     RAGTIQGNVGVLTEALVDNLRENSIGDGVARAMQRLLDLDFVAIYDASGRLLDVFGMR
                     AGQAVALSGGTVVGGAPETVTFETVGPRIALIGAQTVEDGGQALGVIVGGDFVDSDLL
                     ARMLPVGTPGAIYSMNETLLASTFPETSVALLPRADVARITERALRIDVFPLETLTIG
                     GQQYFAAAVTLMQEPITPIATLVVAQPAAVVAETQRGVTRVIFLVALGAAALALLLAL
                     LVSRRITRPVVALTTAATRVQGGDLGVRAETRGEDEVADLGRAFNSMTASVTTMTEEL
                     RDAAEEQARLRARLETVLNSMGDGLIAVNDAGRVVTYNPAAGTILGLPRSRVVGRPVK
                     DVLRGRTADGRPILAGRGATNGLAFVRRQSGDDIPVAITSSPLRDGDGASVGRVYVLR
                     DMTREHQVERMKSEFLSNVSHELRTPLTPIIGYSELLARRGAQPGQAKEFAGGILESA
                     KRLERVVAMLVDFSALEAGRMALSTERVQLGPIIKGVVDAWKGRSERHRFTTKTPRRL
                     PAVDVDFSL"
BASE COUNT          463 a         1189 c         1280 g          512 t
ORIGIN      
        1 cgccacgacg gacccgccgc ccgccgcccc ggccgcggtc gacggacgcg cgccgccgga
       61 ggggcggacg cccaccaaac gaccgcggcg gctgccccgg ctgctccgac gaggatgatc
      121 aaggtctgca tcgaacgcgg atcccctgcg tccattgtta ccccgccctg ccggactatg
      181 acggctggca taccatctcg tcgcagtgga tcgtcgcgtg gtctcggccg tcccggccgc
      241 catcctcctc gcatgcctct acctcgtcga cctgccgttc tacgtcgtgt cgccgggacc
      301 ggcggacgac gcgctgccgc tcatcgacat cgacgggcgt gagacgttcc cgtcgggagg
      361 gcgcctgttg ttcacgacgg tctacgcggg cccgggcaac gtctacgacg tggcgcgcgc
      421 ctccgtcgac gacggcgcac gcgtcgttcc ggagtcgctg ttcctccagc ccggacagac
      481 cgacgccgag tacgacgccc agcagcggtc cgcgatggac cagagcaagc tcgacgcggc
      541 ggccgtcgcg ctccgcgagg tcacgcggta tcccgagcgg cacggccgcg gggccgtcgt
      601 cgagtcggtt ttccgcggga cgccggccga gggtcggctc ttcccgggag atctcgtcat
      661 ctccgcgggg gaccgcgcgg tgcgctctat cgatgatctc gcggatgcga tccagcgggc
      721 gggggccggc ggcgagctgt ccgtcgaagt gcggcccatc gagggcggtg acgtgaggcg
      781 cgtcgtcctc cgaccggcga ggatcggcgg cgacgtagtg atcggggtca gcctcatcga
      841 gaactttccg ttcgacgtgc ggatcgagag cggcgatatc ggggggccgt cggccggcct
      901 catgtgggcg atcggcatcg tggacctgct gaccccgggc gacctgacgg gtggcaggac
      961 gatcgccggc accggtgaca tcgatctgga gggcaacgtg gcgccgatcg gcggcatcac
     1021 cgagaagctc atcgcggcgg agcgcgccgg cgccgagctg ttcctcgtgc cggaggccaa
     1081 catggaagag gcgcgcgcca cgcccgcgtc gatcgatctc gtgtcggtcg ccacgctgga
     1141 cgacgcgctg cactcgctcg agggatgaat ccgacgaccg agacgacgct gctgttcgcc
     1201 gcgcacatgc tggcgctgct ggtcgcgctc ggcacggcgc tggtcctcgg cggagaggtc
     1261 acgaggacgc gaaccgcgcg cgtgaccggc tcgctcggct tcgtctcgat cgcggtcgcg
     1321 gaggcgatcc acggcgccgg gctcgccgcc taaggcgacg cccaggtgct cttgctcagg
     1381 gccgccgggt acctgctgct gctgttcgcg gcggtgatgc cgatcggcat ggaaggcgcc
     1441 ggcgcgttcc tcgcgctgcc ggcgggggcg atcgtcccgg cctccgtcgg gctcgtcgcg
     1501 ggcgcgatga gcggctggcg gcggcggggc gaacgcggag gcgtatggct cggcgcaggc
     1561 gtgatgctgc tcgcgatcgg cgactcgatg ctcgtcgtgg atcgcccgtg ggttctcggc
     1621 ctctcgcacg ccgtgcggtc ggccggctac ctcgcggtcg gctggttcgc gtacgcggcg
     1681 acgcgccaca gcatccgctt ccgcatggtc acgggattcg tcgcgctgct gctcgcggtg
     1741 gtgctcgcga tgtcttcggc cgtcgcgcag gtcatcgcga cgaaccttcg cgagtcggcg
     1801 atcgccagcg tcagccagca gggcgcgaac gccgagaccg agatggtgtc gcgcgccgga
     1861 acgatccagg gcaacgtcgg cgtcctcacc gaggcgctcg tcgacaacct cagggagaac
     1921 tcgatcggcg acggcgtggc gcgagcgatg cagaggctgc tcgacctcga cttcgtcgcg
     1981 atctacgacg cgtcggggcg gctcctcgac gttttcggga tgcgtgccgg acaggcggtg
     2041 gcgctctcgg gcggcacggt cgtcggcgga gcgcccgaga ccgtcacgtt cgagacggtc
     2101 gggccgagga tcgcgctgat cggcgcccag accgtcgagg acggtggaca ggcgctcggc
     2161 gtcatcgttg gcggtgactt cgtggactcg gatctcctcg cccggatgct cccggtcggg
     2221 acgccgggcg ccatctactc gatgaacgag acactcctcg cttcgacgtt cccggagacg
     2281 agcgttgcgc tgctgcctcg agccgacgtc gcgcggatca cggagcgggc gctgcgcata
     2341 gacgtgttcc cgctcgagac gctcacgata ggtggccagc agtacttcgc cgcggccgtg
     2401 acgctgatgc aggagccgat cacgccgatc gcgacgctcg tcgtcgccca gcccgccgcg
     2461 gtggtggcag agacgcagcg gggggttacc cgcgtgatct tccttgtggc gctcggcgcg
     2521 gcggcgctcg cgctgctgct ggcgctgctc gtctcacgac ggatcacgcg gccggtggtc
     2581 gcgctcacga cggccgcgac gcgggtgcag ggcggagacc tcggcgtccg agccgaaaca
     2641 aggggcgagg acgaggtcgc ggacctcggg cgcgcgttca acagcatgac ggcctcggtg
     2701 acgacgatga ccgaggagct ccgcgacgcc gccgaggagc aggcgcggct ccgcgcgcgg
     2761 ctcgagacgg tcctcaactc gatgggggac ggcctgatcg ccgtgaacga cgccggacgc
     2821 gtcgtcacgt acaacccggc ggccggcacg atcctcgggt tgccccgcag ccgcgtggtc
     2881 ggccggccgg tgaaggacgt gctgcgaggc cggacggccg atgggcgtcc gatcctcgcc
     2941 ggccgcgggg cgacgaacgg cctcgcgttc gtcaggcggc agagcggcga cgacatcccc
     3001 gtcgcgatca cgtcgtcacc gctccgcgac ggcgacggcg cctcagtggg gcgcgtgtac
     3061 gtgctccgcg acatgacgcg cgagcaccag gtcgagcgga tgaagagcga gttcctgtcg
     3121 aacgtgtcgc acgagctaag gacgccgctc acgccgatca tcgggtactc ggagctcctg
     3181 gcgcggcggg gggcgcagcc gggtcaggcg aaggagttcg cgggcgggat cctcgagtcc
     3241 gcgaagcggc tcgagcgcgt cgtcgcgatg ctcgtcgact tctccgcgct cgaggccggc
     3301 cggatggccc tgtcgacaga gcgcgtccag ctggggccga tcatcaaggg cgtcgtcgat
     3361 gcgtggaagg gccggagcga gcggcaccgg ttcaccacca agacccctcg ccgcctcccc
     3421 gccgtcgacg tcgacttctc gctc
//
LOCUS       DASZOB010000166         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_74250, whole genome shotgun sequence.
ACCESSION   DASZOB010000166 DASZOB010000000
VERSION     DASZOB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74250"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1502
                     /locus_tag="VG709_02740"
     CDS             183..1502
                     /locus_tag="VG709_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015388828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWC32026.1"
                     /translation="MKKVPAAKVAMAQEGSESPLPAQVQKALGELVGAAREGLLALSI
                     GVGLGVVHEAMEAEVTEVVGPMSKHNADRVAKRHGHEDGSMTLGGRRVQVRRPRIRSA
                     DDEQELPVETYTYFADRDPLTRAVMDRMLAGVSTRKFASVGESIGEGVEDSSSAASKT
                     SVSEMFVERTATALSDLMSRPLDDVRLAVMMLDGIEIADRTHVVALGITTDGVKIPLG
                     LWEGSTENATLARTLLADLVDRGLDPEQAILFVIDGGKALRRAIKDVFGEHALVHRCH
                     RHKERNVTDLLPERDRDQVRARIRAAWSLTDPELPRGRLELLAAELDATWPDAAGSLR
                     EGIDEALTLMRLGISGQLARTLCSTNPCESMIEIVRYTQRNVKCWQDGDMRKRWTAAG
                     MLVAEQQFRRIIGYRDLAKLVIAIERHALLVAPSDLDRQEVAEPVTV"
     gene            complement(1443..>1687)
                     /locus_tag="VG709_02745"
     CDS             complement(1443..>1687)
                     /locus_tag="VG709_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32027.1"
                     /translation="ALPRARRPASQGSVERLDGYLETNFEPGRRFAPELDFALQLDNW
                     RMSGSSLNFGGDHSRLTSHGNGLGDLLTIEVGGGDE"
BASE COUNT          278 a          563 c          599 g          247 t
ORIGIN      
        1 gtgacgctcg gcgggcgccg cgtgccggtg ccctcccgga cttacctgct tctgtgcgaa
       61 tgcagaagga tggaggttgt ccgagatctc gtagaacttg agcggcggtc ctcccgcctc
      121 gtccgctccg gttgggagcg tgcggggcgc aaaccaagtc cgcgaggaca ggaggaaccg
      181 ccatgaagaa ggtacccgcc gccaaggtcg ccatggctca ggagggatct gagagtccgc
      241 tccctgcgca ggttcagaag gcgctcggcg agctcgtcgg ggcagcgagg gagggactgc
      301 tcgcgctcag catcggtgtc gggctcgggg tcgtgcacga ggcgatggag gcggaggtga
      361 ccgaggtcgt cggcccgatg agcaagcaca acgccgaccg ggtcgccaag cgccacggcc
      421 acgaggacgg gtcgatgacg ctcggcggcc gacgcgtgca ggtccgacgc ccgcggatcc
      481 ggtcggccga cgacgagcag gaactgccgg tggagaccta tacgtacttc gccgaccgcg
      541 acccgctgac gcgggcggtg atggaccgga tgctcgccgg cgtctcgacg cgcaagttcg
      601 cgagtgtggg cgagtcgatc ggcgaagggg tcgaggactc cagctcggcg gcgagcaaga
      661 cgtcggtgtc ggagatgttc gtcgagcgca ccgcgaccgc actatcggat ctgatgtcgc
      721 gcccgctcga cgacgtgcgt ctggcggtga tgatgctcga cggcatcgag atcgccgatc
      781 gcacccacgt cgtcgcactc gggatcacga ccgacggcgt caagatcccg ctcgggctgt
      841 gggagggctc gaccgagaac gccaccctgg cgcgcacgct gctggccgac ctggtcgacc
      901 gcggcctcga ccccgagcag gcgatcctgt tcgtcatcga cggcggcaag gccctgcgca
      961 gggcgatcaa ggacgtcttt ggcgagcacg cgctcgtgca tcgctgtcac cgccacaagg
     1021 agcgcaacgt gacggacctg ctgcccgagc gcgaccgcga ccaggtgcgc gcccggatca
     1081 gggcggcgtg gtcgctgacc gaccccgagc tcccccgcgg ccgcctcgag ctgctcgccg
     1141 cagagctcga cgcgacttgg cccgacgccg cgggctcgct gcgcgaaggg atcgatgagg
     1201 cgctgacctt gatgcgcctc ggaatcagcg gccagctcgc ccgaacgctg tgctcgacga
     1261 acccctgcga gtcgatgatc gagatcgtcc gctacaccca gcgcaacgtg aagtgctggc
     1321 aggacggtga catgcgcaag cgctggaccg ccgccggcat gctcgtcgcc gagcagcagt
     1381 ttcggcggat catcggctac cgcgacctcg ccaagctcgt catcgcgatc gagcgtcacg
     1441 ccctactcgt cgccccctcc gacctcgatc gtcaggaggt cgccgagccc gttaccgtgt
     1501 gacgtcaacc gggagtgatc gccgccgaag ttcaacgacg atccggacat cctccagttg
     1561 tcgagctgga gggcgaagtc gagctctggg gcgaagcgac ggccgggctc gaagttcgtc
     1621 tcgaggtagc catccagtcg ctcgacgctc ccttgacttg cgggtcggcg ggcgcgcgga
     1681 agcgcca
//
LOCUS       DASZOB010000167         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_113643, whole genome shotgun sequence.
ACCESSION   DASZOB010000167 DASZOB010000000
VERSION     DASZOB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113643"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VG709_02750"
     CDS             <1..241
                     /locus_tag="VG709_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32028.1"
                     /translation="RVDVVRFDEDDPSAAFARLGPLTRSHPDEPVVRFHLGLLLVSLG
                     QVAEAREQLAHAARGEGLYGREARRLLTRLEEIRT"
     gene            427..1470
                     /locus_tag="VG709_02755"
     CDS             427..1470
                     /locus_tag="VG709_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC32029.1"
                     /translation="MADHEEATRALVRQGQEAGFLTHDEIALALDELDLDTAQVDDFY
                     ASLDELHIEVLDSPDDREAVLDLEPEASEVSTDSLQLFLKDIGKVRLLTAAQEVELSK
                     RIERGDHRAKQEMVAANLRLVVSIAKNYRNQGLPFLDLIQEGTIGLVRAAEKFDHRKG
                     FKFSTYATWWIRQAVARALADKARTIRMPVHVVEKLNKIGRAERKLLSEFGREPTSGE
                     IAVELELECDEVEQIRMSAQAPVSLEKPVGDEEESEFGHFLTDETQPLPDEVAEETMR
                     KEALRSILGSLSPREQRVLELRYGLDGQQPRTLDEVGRTFNVTRERIRQIENQSLKKL
                     RALADAGRLRDVA"
BASE COUNT          267 a          514 c          552 g          204 t
ORIGIN      
        1 acgcgtcgac gtcgtccgct tcgacgagga cgacccctcc gccgcgttcg cgcgcctcgg
       61 cccgttgacg cgctcgcacc ccgacgagcc cgtcgtccgc ttccacctcg ggctcctgct
      121 cgtctcgctc gggcaggtcg ccgaggcgcg cgagcagctg gcgcacgccg ctcgcggcga
      181 agggttgtac ggacgggaag cgagacgact cctcacgcgg ctggaagaaa ttaggacttg
      241 agactcacaa agatgggccg aacggcctat gcacgccgtc cggtacgcgt gggaaagtgc
      301 cgcggtcacg agcgtgaatc gccacacacc ggccgttgac ggcgcagcga cttggcccta
      361 cggtcagcga ggagggccgg cgtgagccgg gggaacggga ccgagggaaa cggagcggca
      421 gcagtggtgg ctgaccacga ggaggcgacg agggcgctcg tccgccaggg gcaggaggcg
      481 ggcttcctga cgcacgacga gatcgcgctt gcgctcgacg agctcgacct cgacacggcg
      541 caggtcgacg acttctacgc gtcgctcgac gagcttcaca tcgaggtgct cgactcgccg
      601 gacgaccgtg aggcggtcct cgacctcgag cccgaggcga gcgaggtctc gaccgactcc
      661 ctccagctct tcctcaagga catcggcaag gtgcggctgc tcaccgccgc acaggaggtc
      721 gagctctcga agcggatcga gcgcggcgac caccgggcga agcaggagat ggtcgcggcc
      781 aacctccgcc tcgtcgtgtc gatcgcgaag aactaccgca accagggtct tcccttcctc
      841 gacctgatcc aggaggggac gatcggcctc gtgcgcgcgg ccgagaagtt cgaccaccgc
      901 aaggggttca agttctcgac ctacgccaca tggtggattc ggcaagccgt cgcgcgcgcg
      961 ctcgccgaca aggcgcggac gatccggatg ccggtgcacg tcgtcgagaa gctcaacaag
     1021 atcgggcgcg ccgagcggaa gctcctgtcc gagttcggac gcgagccgac ctccggcgag
     1081 atcgcagtcg agctggagct cgaatgcgac gaggtggagc agatccgcat gagcgcccag
     1141 gcgccggtgt cgctcgagaa gccggtcggc gacgaggagg agtcggagtt cgggcacttc
     1201 ctcaccgacg agacgcagcc gctgcccgac gaggtggccg aggagacgat gcgcaaggag
     1261 gcgctgcggt cgattctcgg ctcgctgtcg ccgcgggagc agcgcgtgct cgagctgcgg
     1321 tacggtctcg acggccagca gccgcggacg ctcgacgagg tgggccgcac gttcaacgtg
     1381 acgcgggagc gaatccgcca gatcgagaac cagagcctca agaagctgcg tgcgctcgcc
     1441 gacgccggca ggctgcgcga cgtcgcgtag cgcgctccgg cggggccggc tcggcccggg
     1501 ccactagact cgcgtagact cgcgcgcggt gccgact
//
LOCUS       DASZOB010000168         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_64383, whole genome shotgun sequence.
ACCESSION   DASZOB010000168 DASZOB010000000
VERSION     DASZOB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64383"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VG709_02760"
     CDS             complement(<1..444)
                     /locus_tag="VG709_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWC32030.1"
                     /translation="MADLGDLCRAALAQVRDGEEAEAYAVQSRRSEVKVRGGTVESLS
                     SSETRGVGVRVVIDGGLGYAWAADPDDGEAADLIEKAREGARHATPDDANVLPEPRDA
                     APIAALYRDRQPDVAPARKVSLALDLERVATRARPDVRRVEDAVYG"
     gene            complement(437..1816)
                     /locus_tag="VG709_02765"
     CDS             complement(437..1816)
                     /locus_tag="VG709_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWC32031.1"
                     /translation="MLDSSLVQDVLRVALSTGGDVAEVFAEERSSTSLRLDDRRVEEL
                     TTGLDRGGGIRVVAGDTTAYAYSNRLTEDRLRAAAETAAAAVRGRSTAGLRAFDLRDG
                     KPTVMNAARAVSEERKVDWVREADDAARSHSPRVRQVVVAYGDSAQRLLVASSDGTWV
                     EEDRHRVRLVVSVVAARDGVRQTGFEGPARLGGIEMLDEHPPAEIARKAARMAVTMLD
                     GVPAPAGEMAVVVGPAGGGVLFHEACGHGLEADTVGKEASIYRGRIGEKLGSPLVTGV
                     DDSTVEGAWGSFVFDDEGTPAERTVLVDEGVLTGLLYDRFWARKDGGRPTANGRRQSY
                     AHLPIPRMTNTSILPGADDPARIIADTKRGLYAKTLGGGQVNPATGDFVFGVSEGYLI
                     EDGEITRPVRGANLIGNGITILGAIDAVASDFDARPGTCGKDGQGVPVTTGSPTLRIA
                     RMTVGGTGG"
     gene            1869..>3391
                     /locus_tag="VG709_02770"
     CDS             1869..>3391
                     /locus_tag="VG709_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32032.1"
                     /translation="MARAVSSGTSPPAAPERGRTASWVRACAFAAASGGAVLAMYLAH
                     PLLSGFDLPVGPDGPVYVWWTRYAGGAGLEAVPGARPGLPALALALQVFLRTDAVGAV
                     TVLGPVLAATSGLAGAALLEAALGPRVVRAAAAPLMPGAFAAYLAGGWLGNTALVALF
                     LAAAAALALARASWRAVAGAAALLAAAAVAHRIAIVLVLTVLGGVVLTHVPEALRERR
                     RGVRGRDTFAVRAGVAVAAAAGVAAAALLPALGAPRLPGDTSQDGFFRRHGLRALLVD
                     RYRERLGGDAARALIPITTGLGLGAIGVGRRPSRPGERFLLSLVASWAAITIVAVALF
                     AATAGAPPNRVLVFAFFIPLAAAAGVSWAWRRAPLALAALASVAAVAFVGVSMLGWAR
                     QSPAFSSDELTAAREAGRTAASLPPGTPLVFAVDTFEPAAAYHVTRAFNVIRAGLPPE
                     RIPDAHVVVGAPEDVVAGRTTTNGDPEHDAIARAYLAETSEATRSRPPFVFVVEAFNP
                     "
BASE COUNT          380 a         1303 c         1221 g          487 t
ORIGIN      
        1 cccgtagacg gcgtcctcga cgcggcggac gtctggacgc gcgcgggtcg cgacgcgctc
       61 gagatcgagg gcaagcgaca ccttccgggc gggagcgacg tcgggctgcc ggtcgcggta
      121 cagcgccgcg atcggggccg cgtcccttgg ctccggaagc acgtttgcgt cgtcgggcgt
      181 ggcgtggcgc gcgccctcac gcgccttctc gatgaggtcc gcggcttcac cgtcgtcggg
      241 atcggcggcc cacgcgtacc cgagcccgcc gtcgatgacg acgcggacgc ccacgccacg
      301 cgtctcagag gaagagagcg actcgaccgt cccgccgcgc accttgacct cgctccgtcg
      361 gctctggacc gcataggcct ccgcctcctc gccgtcgcga acctgcgcga gcgcggcgcg
      421 acagaggtcg ccgagatcag ccaccggtcc ctcccacggt catccgcgcg atccgaagcg
      481 tcgggctccc cgtcgtgacg ggaacgccct ggccgtcctt gccgcacgta cccggacgcg
      541 cgtcgaagtc cgaggcgacg gcgtcgatcg caccgaggat cgtgatcccg ttcccgatga
      601 ggttggcgcc gcggaccggc cgtgtgatct cgccgtcctc gatcaggtat ccctcggaga
      661 ccccgaacac aaagtcgccg gtggccgggt tcacctggcc cccacccaac gtcttggcgt
      721 agagccctcg cttcgtgtcg gcgatgatcc gcgccgggtc gtcggcgccc ggcaggatcg
      781 acgtgttcgt catgcgcggg atcgggaggt gcgcgtacga ctggcggcgg ccgttcgcgg
      841 tcggcctgcc gccgtccttc cgggcccaga agcggtcgta gaggagtccc gtgagcacgc
      901 cctcgtcgac gagcacggtt cgctccgcgg gcgtcccctc atcgtcgaac acgaacgatc
      961 cccatgcgcc ctctacggtc gagtcgtcga cgcccgtcac gagcggtgag ccgagcttct
     1021 ccccgatccg accgcggtag atcgaggcct ccttcccgac ggtgtcggcc tcgagcccgt
     1081 ggccgcatgc ctcgtggaac agaacgcctc ctccggcagg cccgacgacg accgccatct
     1141 cccccgccgg cgccgggacc ccgtcgagca tcgtcacggc catacgcgcc gccttccgag
     1201 cgatctcggc cggcgggtgc tcgtcgagca tctctatgcc gccgagccgg gccgggccct
     1261 cgaagccggt ctggcggacg ccgtcacgcg cggcgacgac cgagacgacg agtcggactc
     1321 ggtgccggtc ctcctcgacc cacgtgccgt cggagctcgc gacgagcagg cgctgtgcgc
     1381 tgtcgccgta cgcgacgacg acctggcgga cccgcggcga gtgcgaccgg gcggcgtcgt
     1441 cggcctcgcg gacccagtcg accttgcgct cctcggaaac ggcgcgcgcg gcgttcatga
     1501 ccgtcggctt cccgtcacgc aggtcgaacg cgcgcagccc tgcggtcgat cggccgcgga
     1561 ccgccgccgc cgcggtctcg gcggccgcgc gtagccggtc ttcggtcagc cggttcgagt
     1621 acgcgtacgc ggtcgtgtcg ccggcgacga cgcggatccc gccaccccgg tcgagccccg
     1681 tcgtcagctc ctcgaccctg cggtcgtcga ggcggagcga cgtcgagctc cgctcctccg
     1741 cgaagacctc ggcgacatcg ccgcccgtcg agagcgccac gcgcaggacg tcctgcacga
     1801 gcgacgagtc gagcatggcg agggattcta gcctcgggac tgcgaccgag cctgtacggt
     1861 gctggctcgt ggcgcgcgcc gtctcgagcg ggacctcgcc ccccgccgcg cccgagcggg
     1921 gccgaaccgc ctcgtgggtg cgcgcgtgcg cgttcgccgc cgcgtccggc ggggcggtgc
     1981 tcgcgatgta cctcgcgcac ccactgctct ccgggttcga tctcccggtc ggcccggacg
     2041 ggcccgtgta cgtctggtgg acgcgctacg cgggcggcgc cgggctcgag gccgtccccg
     2101 gagcgcggcc cggactgccg gccctggcgc tcgcgctcca ggtcttcctc cgcacggacg
     2161 cggtcggggc cgtcacggtc ctcggacccg tgctcgccgc cacatcgggg ctcgcgggag
     2221 cagcgctgct ggaggcggcg ctgggaccgc gcgtcgtccg cgcggcggcc gcgccgctca
     2281 tgcctggggc gttcgcggcc tacctcgcgg gcgggtggct cgggaacacg gcgctcgtgg
     2341 cgctgttcct cgcagccgcc gccgcgctcg cgctcgcgag ggcatcgtgg cgagcggtcg
     2401 caggggcggc ggcgctgctc gccgccgcag cggtcgccca ccggatcgcg atcgtgctcg
     2461 tgctgaccgt gctcggaggc gtggtcctca cgcacgtgcc cgaggcgctc cgtgagcgac
     2521 ggagaggagt tcgaggccgc gacacgttcg ccgtccgtgc cggcgtcgcc gtggcggcgg
     2581 cggcgggcgt ggccgccgcg gcgctcctgc cggcgctcgg agcgcctcgg cttccaggcg
     2641 acacgtctca ggacggcttc ttccgccggc acgggctccg cgcgctgctc gtcgaccggt
     2701 atcgcgagcg gctcggcggc gacgccgcac gcgcgctgat ccccatcacc acgggcctcg
     2761 ggctcggagc gatcggcgtc gggcggcgcc cgtcccgacc gggagagcgg ttcctgctat
     2821 cgctcgtcgc gtcgtgggcg gccatcacga tcgtggccgt cgcgctgttc gcggctaccg
     2881 caggggcacc gccgaaccgc gtgctcgtgt tcgcgttctt catcccgctc gcggcggccg
     2941 ccggcgtctc gtgggcgtgg aggcgggccc ccttggcact cgccgccctc gcgagcgtag
     3001 cggcggtcgc gttcgtcggc gtgtcgatgc tcggatgggc gcgccagtcg cccgcgttct
     3061 cgtccgacga gctcacggcg gcgcgcgagg cggggcgaac ggccgcctcg cttcctcccg
     3121 ggacgccgct ggtcttcgcc gtcgacacgt tcgagcccgc ggccgcctac cacgtcacac
     3181 gggccttcaa cgtgatccgc gccgggctgc cgcctgagcg catccccgac gcacacgtcg
     3241 tcgtcggcgc gcccgaggac gtcgtcgcgg gccggacgac gacgaacggc gaccccgagc
     3301 acgacgcgat cgcgcgcgcg tacctcgcgg agacgtccga agcgacgcgg tcgcgaccgc
     3361 cgttcgtctt cgtcgtcgag gccttcaacc c
//
LOCUS       DASZOB010000169         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_23863, whole genome shotgun sequence.
ACCESSION   DASZOB010000169 DASZOB010000000
VERSION     DASZOB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23863"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VG709_02775"
     CDS             complement(<1..1127)
                     /locus_tag="VG709_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWC32033.1"
                     /translation="MFSRILIANRGEVAVRVIRACQELGVETVAVYSTADRESLHVEL
                     ADRAVHIGPPPPSESYLNVASLIAAATTTGCEAVHPGWGFLAESAPFAAACEENDLVF
                     VGPRPETVAAMGDKVRAKETVAAAGVPVVPGSPGTVDLAGARELAARLSFPLLLKAAA
                     GGGGRGMRLVASDEDLERAFQTAAAEAEAAFSNGSLYIEQALVGARHVEIQVLGDGVG
                     GVLTLGERDCSIQRRHQKLIEEAPSPAVSPDLRAEMEAAAATACEALRYRGAGTLEFL
                     LDPDGRFYFIEMNTRLQVEHPVTELLTGVDLARAQLHIAAGDGLARTGRAALSGHAIE
                     IRINAEDPARGFLPAPGTVTRFEPPLGPGVRVDTHVYDGYR"
     gene            complement(1258..1539)
                     /locus_tag="VG709_02780"
                     /pseudo
     CDS             complement(1258..1539)
                     /locus_tag="VG709_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
BASE COUNT          224 a          642 c          619 g          241 t
ORIGIN      
        1 atccggtagc cgtcgtagac gtgcgtgtcg acccggacgc ccggaccgag cggcggctcg
       61 aaccgcgtca ccgttccggg agccggcagg aagccgcgcg cggggtcctc ggcgttgatc
      121 cggatctcga tcgcgtgtcc cgagagggcc gcgcggccag ttcgcgcgag gccgtccccc
      181 gccgcgatgt gaagctgagc gcgcgcgagg tcgacgccgg tcaggagctc cgtgacggga
      241 tgctcgacct gtagccgcgt gttcatctcg atgaagtaga agcggccgtc ggggtcgagc
      301 aggaactcga gcgtcccggc cccgcggtag cggagcgcct cgcacgccgt tgcggcggcc
      361 gcctccatct ccgcgcgcag atccggcgag acggccggcg acggcgcctc ctcgatcagc
      421 ttctggtgcc gccgctggat cgagcagtcg cgctctccca gggtgagcac gcctccgacg
      481 ccgtccccga gcacctggat ctcgacgtgg cgggcaccga cgagcgcctg ctcgatgtac
      541 agcgagccgt tcgagaacgc agcctcggcc tccgccgccg ccgtctggaa cgcacgctcg
      601 aggtcctcgt cgctcgcgac gagccgcatg ccgcgcccgc cgccgcccgc ggcggccttg
      661 aggagcaacg ggaacgagag gcgagcggcg agctcgcgtg cgccggcgag gtcgaccgtg
      721 ccgggcgagc cgggcacgac cggcacgccc gccgccgcca ccgtctcctt cgcccgcacc
      781 ttgtcgccca tcgcggccac cgtctccggg cgcgggccga cgaagacgag gtcgttctcc
      841 tcgcacgcgg cagcgaacgg ggcactctcg gcgaggaacc cccagccggg gtggacggcc
      901 tcgcagccgg tggtcgtcgc cgcagcgatg agcgaggcca cgttgaggta gctctcgctc
      961 ggcggcggcg ggccgatgtg gacggcgcga tctgcgagct cgacgtggag cgattcgcga
     1021 tcagccgtcg agtacaccgc gaccgtctcg acgccgagct cctggcaggc gcggatgacg
     1081 cggacggcga cctcgccgcg gttcgcgatc aggatgcgcg agaacacggg cgctcgcgct
     1141 cggtgtcgct tcgggcgcct ttcgtcgcgg tgtgcgggcc acgcgcgcgc ttccgggcga
     1201 tacgctcgca gcggagggag gccagcggcg gccccgggaa cgtgtcccgg cgggacacta
     1261 gagcgcgtcc agcggccggc cgttcaccgg ctcgagctcg aacaggagct gcccgtactc
     1321 gaccggctcg gcgttgtcga cgcagatgcg ctgtacgacc gcctcgacgt ccgcctgcac
     1381 ctcgttcatg agcttcatcg cctccagaat gcagagcgtc tgtcccgggg cgaccgggtc
     1441 tccgacctcg acgaacggcg gcgcgcccgg ttccggcgcg cggtagaacg tgcctaccat
     1501 cggtgcctcg acgcggacga tgtcgagtcg cgacggggcc gccgtcgccg cctcgtcggc
     1561 cggcgggcgc gatgccgagc gtggcgacgg gcgcctcgcc gctctcgtcc gtcctccgga
     1621 tcgtcacgcg cgtctccccc tcctcgatcg tcacctcgcc gatccccgac tcctgcacca
     1681 cgcggatgag atcgcggatt cgttcggtct cgctccgctc gagccc
//
LOCUS       DASZOB010000170         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_74269, whole genome shotgun sequence.
ACCESSION   DASZOB010000170 DASZOB010000000
VERSION     DASZOB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74269"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VG709_02785"
     CDS             complement(<1..410)
                     /locus_tag="VG709_02785"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWC32034.1"
                     /translation="MVSVSPFEALLYDPRRVGDLASVTSPPYDVVLPHEQAALHSSSP
                     YNIALVDLGGEDGGAPNGKYTRAARLLRRWREERILVPTDGARVYPSEMRFRYFGEGR
                     RIRGVILAVGLEPWGGGILPHEQTMKGPIEDRLQL"
     gene            450..800
                     /locus_tag="VG709_02790"
     CDS             450..800
                     /locus_tag="VG709_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32035.1"
                     /translation="MTDTYELFAKGHEFLASGRPHQAVVVLERAATAEPEKGSIREAL
                     ARALYLTGRWSRARSEFAKAVDIDPVNHYAHFGLGLSCAKTGDRMQAIAHLKLALAME
                     PLEEYRAALERLTS"
     gene            805..>1663
                     /locus_tag="VG709_02795"
     CDS             805..>1663
                     /locus_tag="VG709_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWC32036.1"
                     /translation="MRPAERYEAFLLDLDGVVYRGTEALPGAAEAITELKHAGRRVLF
                     LTNNSARTAAQVADRLVAMGIDAAPEDVLTSAQATAAMIAADARRSGRPATAFVVGEE
                     GLDGALRDAGIDTRDGEAPGYVVVGWDRGIDFGKLRRASVLVRSGATLVASNADATYP
                     APGGELWPGAGSLLAAVETASGRRADVVGKPHRPMFDAAVERVATRDALVVGDRLETD
                     VAGAVGAGLDAAFVLSGAGTLADLPDHEAQPHLVLRNLAAVLDERCDARLRRARPEDA
                     DAVGSLLEAA"
BASE COUNT          237 a          572 c          633 g          221 t
ORIGIN      
        1 agctggagcc ggtcctcgat ggggcccttc atcgtctgct cgtgcggcag gatgcctccg
       61 ccccacggct cgagcccgac ggcgaggatc acgccgcgga tcctccgccc ctccccgaag
      121 tagcggaagc gcatctcgga ggggtagacg cgcgcgccgt cggtcgggac gaggatccgc
      181 tcctcgcgcc accggcggag gaggcgagcg gcgcgcgtat acttgccgtt tggagccccg
      241 ccgtcctcgc ccccgaggtc gaccagcgcg atgttgtacg ggctcgaaga gtggagcgcg
      301 gcctgctcgt gcggcagcac gacgtcgtag ggcggcgacg tgacagaggc gaggtcaccg
      361 acgcgtcgcg ggtcgtagag cagcgcctcg aagggggaga cggataccac gggtgtagta
      421 aacggccaag gggacttctg cgcgaaccga tgaccgacac gtacgagctc ttcgccaagg
      481 gacacgaatt cctcgcgtcg gggaggccgc accaggccgt cgtcgtcctc gagcgcgccg
      541 cgacggcgga gccagagaag gggtcgatcc gagaggcgct ggcgcgcgcc ctctacctca
      601 ccgggaggtg gtcgcgcgcg cgcagcgagt ttgccaaggc cgtcgacatc gatcccgtga
      661 accactacgc gcacttcggg ctcggcctct cgtgcgcgaa gaccggcgat cggatgcagg
      721 cgatcgcgca cctcaagctc gcgctcgcga tggagccgct cgaggagtac cgggcggcgc
      781 tggagcggct gacttcctga ggccatgcgt cccgccgagc ggtacgaggc gttcctgctc
      841 gacctcgatg gcgtcgtcta ccggggaacc gaggcgctcc caggagccgc agaggcgatc
      901 accgagctga agcatgcggg acgccgcgtt ctgttcctca cgaacaactc cgcgaggacg
      961 gcggcgcagg tggcagaccg gctcgtcgcg atgggcatcg acgcggcgcc ggaggacgtc
     1021 ctcacgtcgg ctcaggccac cgcagccatg atcgccgccg acgcgcgacg ttcgggacgg
     1081 ccggcgacgg cgttcgtcgt gggggaggaa gggctcgacg gcgcgctccg cgacgccggg
     1141 atcgacacgc gagacggcga ggcgcccggg tacgtcgtcg tcgggtggga ccgcggcatc
     1201 gacttcggga agctgcgtcg ggcgagcgtc ctcgttcggt ccggagccac gctcgtcgcc
     1261 tcgaacgccg atgcgacgta cccggcaccg ggcggggagc tgtggccggg cgcgggctcc
     1321 ctgctcgcgg ccgtcgagac cgcgtcgggg cgccgggccg acgtggtcgg gaaaccgcac
     1381 cggccgatgt tcgacgcggc cgtcgagcgc gtcgcgacgc gggacgcgct tgtcgtcggc
     1441 gaccggctcg agaccgacgt agcgggcgcc gtcggggccg ggctggacgc ggcgttcgtg
     1501 ctctctggcg ccggcacgct cgccgacctt cccgatcacg aggcgcagcc tcacctggtg
     1561 ctgcggaacc tcgcggccgt gctcgacgag cgctgtgatg cgcgccttcg ccgcgcgcgg
     1621 ccggaggacg ccgacgcggt ggggtcgctc ctcgaagcgg ccg
//
LOCUS       DASZOB010000171         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_23869, whole genome shotgun sequence.
ACCESSION   DASZOB010000171 DASZOB010000000
VERSION     DASZOB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23869"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VG709_02800"
     CDS             complement(<1..872)
                     /locus_tag="VG709_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWC32037.1"
                     /translation="MIQTTVDGRYQIITRVASGGMGEVYRAHDAVLDREVAIKVLHSS
                     LASDAGFIDRFRREARAAAMLGHPNIVAVHDWGATEGTYFMVMEFVRGHSLRSILART
                     GRLAPAQAADVTMQILSALDHAHDRGIVHRDIKPENVLVTLDGTVKVADFGLARALAE
                     SAVTHAPGTVTGTVQYLAPEQVQGDPADPRTDLYALGIVLFELLTGRVPFTGETSVAI
                     AYKHLSEPVPPPSRVNPSVPASLDRVVAQATEKERDRRPSSATAMRRDVVRAVSSLPP
                     ARSVAELARSIPPA"
     gene            1045..>1830
                     /locus_tag="VG709_02805"
     CDS             1045..>1830
                     /locus_tag="VG709_02805"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWC32038.1"
                     /translation="MPVTRAKGGASQTPPQLSDDPLVIAGRAFGSRLILGTGKFASHE
                     VMARAIDASGAEIVTVALRRVDLDATGGPDILEFIDAARVLLLPNTSGAVDADEAVRL
                     ARLARTAGMPAWVKLEVTPDPRYLLPDPVETLRAADALVAEGFTVLPYINADPVLAKR
                     LEEAVCATVMPLGSWIGSNQGVRTRDAVGIIVEQATVPVVVDAGLGAPSHAAEAMEMG
                     VDAVLVNTAVAVARDPVRMAEAFRLGVEAGRSAFLAGRAAPRRS"
BASE COUNT          232 a          648 c          706 g          244 t
ORIGIN      
        1 tcggccggcg ggatcgaacg cgccagctcc gccaccgagc gcgcaggcgg gaggctcgag
       61 acggcgcgga cgacgtcgcg ccgcatcgcc gtcgccgagg acggccggcg gtcgcgctcc
      121 ttctcggtcg cctgcgcgac cacgcggtcg agcgacgcgg ggaccgaggg gttgacgcgc
      181 gagggcggcg ggaccggctc ggagaggtgt ttgtacgcga tcgccacgga cgtctctccg
      241 gtgaacggga cgcggccggt caagagctcg aacaggacga tgccgagcgc gtagaggtcc
      301 gtccgcgggt cggccggatc gccctgcacc tgttcgggtg cgagatactg gacggtcccc
      361 gtcacggtgc ccggcgcgtg ggtcaccgcg gactccgcca gcgcgcgggc gaggccgaag
      421 tcggcgacct tgacggtgcc gtcgagcgtc acgagcacgt tctcgggctt gatgtcccgg
      481 tgcacgatcc cgcggtcgtg cgcgtggtcg agcgccgaca ggatctgcat cgtcacgtcg
      541 gcggcctgcg cgggcgcgag gcggccggtg cgggcgagga tcgagcggag actgtggccc
      601 cgcacgaact ccatcaccat gaagtacgtg ccctcggtcg cgccccagtc gtggacggcg
      661 acgatgttcg gatggccgag catcgcggcg gcgcgggcct cgcgccggaa ccggtcgatg
      721 aaccccgcgt ccgaggcgag gctcgagtga agcaccttga tggcgacctc gcggtcgagc
      781 acggcgtcgt gcgcgcggta gacctcgccc atgccgccgg acgcgacgcg ggtgatgatc
      841 tggtaccggc cgtcgacggt ggtctggatc atgcagggcc tgcctcgggg gagcggtccg
      901 cggtcattgt agggccggtc cccgctccga ccggggagcg cggcgcccgc gcgtatactc
      961 ggatcgaacc accggggagc tcggggaacc gggctgagag gccggacggg gcgcgagccc
     1021 gccgccggcg acccgcatga cctgatcccg gtaacgcggg cgaagggagg agcctcgcag
     1081 accccgccgc agctctcgga tgacccgctc gtcatcgccg gccgcgcgtt cggctcccgg
     1141 ctgatcctcg ggaccggcaa gttcgcctcg cacgaggtga tggcgcgcgc gatcgacgcg
     1201 tccggggcgg agatcgtcac ggtcgcgctc cgccgggtgg atctcgacgc gaccggcggc
     1261 cccgacatcc tcgagttcat cgacgccgcg cgcgtgctgc tgctgccgaa cacgtcgggc
     1321 gcggtggacg ccgacgaggc ggtccgcctg gcgcggctgg cgcgcaccgc cggcatgccc
     1381 gcgtgggtga agctcgaggt cacgcccgac ccgcgctacc tgctgcccga tcccgtcgag
     1441 acgctgcgcg ccgccgacgc gctggtcgcg gaggggttca ccgtgctgcc gtacatcaac
     1501 gccgacccgg tgctcgccaa gcgcctggag gaggccgtct gcgcgaccgt catgccgctc
     1561 gggtcgtgga tcggctcgaa ccagggcgtc cgtacgcgcg acgccgtggg gatcatcgtc
     1621 gagcaggcca ccgtcccggt cgtcgtcgac gcggggctcg gtgcgccgtc ccacgccgcc
     1681 gaggcgatgg agatgggcgt cgacgccgtc ctcgtcaaca ccgccgtcgc ggtcgcgcgg
     1741 gaccccgttc ggatggccga ggccttccgg ctcggcgtcg aggccgggcg gtcggcgttc
     1801 ctcgcaggcc gcgcggcccc gcgccggtct
//
LOCUS       DASZOB010000172         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_64478, whole genome shotgun sequence.
ACCESSION   DASZOB010000172 DASZOB010000000
VERSION     DASZOB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64478"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..88)
                     /locus_tag="VG709_02810"
     tRNA            complement(4..88)
                     /locus_tag="VG709_02810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ser,seq:tga)
     gene            complement(590..1351)
                     /locus_tag="VG709_02815"
     CDS             complement(590..1351)
                     /locus_tag="VG709_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWC32039.1"
                     /translation="MEQLVSDLVHLTPEEYKVLDEDDFGVPGLVAHEVIGPFVRVQAL
                     GTLIMVHDGRFEPRHGIGHHPHRFNERLFYILEGAVDHDDALNGIQGHMGTGDVGRLT
                     EGRYGMIHKEWNNTDGPGRAFILVYSTEPTPARASFELLADADAPRYDEASGVRTKEL
                     VGPKVKFPLHGDVRFFAASEIAADGELRLETSDGEAGMLFTLDGELDVDGTALGKEHT
                     LLVPPNGSRSVTARAPSGARAIRVVTGPGHGFVTR"
     gene            complement(1383..2657)
                     /gene="serS"
                     /locus_tag="VG709_02820"
     CDS             complement(1383..2657)
                     /gene="serS"
                     /locus_tag="VG709_02820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWC32040.1"
                     /translation="MLDLRAIRDDPESFRDGLARRAPSLADDLDRVVDLDSERRRLTA
                     SVESLRAEQNRASKAIGAAAPEDRGALIASVRDVSTRIEELEPRLAAIDEELRIVLSR
                     LPNVPHPSVPRGEIDEDNDVLREVGAPTSFSFEPRDHVDLGQALGIIDLERAARVSGA
                     RFAYLAGGAVLLQFALVRFCLDRVIAKGFLPIVPPVLVREEAMYGTGFFPTDEAQIYA
                     TRDDDLYLVGTSEVPLAGLHQTEILDPTTLPRRYVGYSTCFRREAGAYGKDTRGIFRV
                     HQFDKVEMFSFTEPDASWDEHEFLLSCEEEIVGALGLPYRVVSVCTGELGASAAQKYA
                     IEVWLPGQGRYRELTSCSNTTDYQARRLECRVRYPEGNRPAHTLNGTAAPIGRTLIAI
                     LENFQREDGSVDVPDALRPYLPESSQVLRPPE"
     gene            complement(2658..3182)
                     /locus_tag="VG709_02825"
     CDS             complement(2658..3182)
                     /locus_tag="VG709_02825"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HWC32041.1"
                     /translation="MVLSPQTLSILVVVSLGLAALAVFLVVASAPRRTGRRPRAATPG
                     EALAAHEHAIDELKRVFRGLQAEQRRQAEQLLVTIGRVGLVRYDAFEDMGGHLSFSAA
                     LLDEQGDGLVVTSINGRQDTRCYAKPVERGTSRYNLSAEEEEAVRQALASRRELGAPV
                     EVPPRRGRRITSRT"
BASE COUNT          524 a         1155 c         1155 g          536 t
ORIGIN      
        1 tcgcggaggg ggtgggattc gaacccacgg ggggttgccc cccagcagtt ttcaagactg
       61 ccaccttcgt ccgctcggtc acccctccag ggggaacccg gattgagtgt cgactgtccg
      121 ggttccacga tctccgcccc gccaagcatg tctcaagcaa gaactcttcg ccggtattct
      181 tcagcacata aagccagtcg taagcgcctg accccaggtc gcttacaacg tagcccttcg
      241 agttggagtg tatccgtcta agggccacct gatgagagct cgtgtaacga tcttgcactc
      301 gcttgatcac ctcgcctgtg tcaacgaata cgtcatatgc cgccgactcc gttagcggga
      361 tgcccacgtc gtatcccatc ttcgtgaagg tcgcaatggc ttgcgcgaca gccatgtctc
      421 ccttccgttg ggtcttcctc actaggacgt catcgtagcg gtaggcagtg acatcgagat
      481 cactcgaccc acaagacaag gacagctccg ctgggtaacg cgcgctcggt ggtccgatgg
      541 tcgcggtcaa cacgccgtcc tgacgatcga tcgtaagaac gacgtagcgc tatcgggtca
      601 cgaagccgtg gccggggccg gtcacgacgc ggatcgcgcg ggcgcccgac ggggcgcgcg
      661 ccgtcacgga gcgagagccg ttcggcggaa cgagcagcgt gtgctccttg ccgagcgctg
      721 tcccgtcgac gtcgagttca ccgtcgaggg tgaacagcat cccggcctca ccgtcagacg
      781 tctcgaggcg gagctcaccg tccgcggcga tctcgctggc ggcgaagaag cgcacgtcgc
      841 cgtggagcgg gaacttgacc ttcggcccga cgagctcctt ggtacggacc ccgctcgcct
      901 cgtcgtagcg cggtgcgtcg gcgtccgcga ggagctcgaa cgacgcgcgc gccggcgtcg
      961 gctccgtcga gtacacgagg atgaacgccc ggccgggacc gtcggtgttg ttccactcct
     1021 tgtggatcat tccgtagcgg ccctcggtga ggcgtcccac gtctccggtt cccatgtggc
     1081 cctgaatccc gttgagcgcg tcgtcgtggt cgacggcgcc ctcgaggatg tagaagagcc
     1141 gctcgttgaa ccggtgcggg tggtggccga tgccgtgccg cggctcgaac cgcccgtcgt
     1201 ggaccatgat cagcgtgccg agcgcctgca cgcgcacgaa cggcccgatc acctcatggg
     1261 cgacgagccc cgggacgccg aagtcgtcct cgtcgaggac cttgtactcc tcgggcgtca
     1321 ggtgaacgag atccgagacg agctgttcca tcgcaggtat tcctaccgcc ggagcgcggc
     1381 cgttattccg gcggcctcag gacctgcgag gactccggga gatacggccg gagcgcgtcc
     1441 ggtacgtcca ccgagccgtc ctcgcgctgg aagttctcga ggatcgcgat cagcgttcgc
     1501 ccgatcggcg ccgctgttcc gttgagggtg tgcgccggcc ggttcccctc cggataccgg
     1561 acgcggcatt cgaggcgccg cgcctggtag tcggtggtgt tcgagcacga cgtgagctcg
     1621 cggtagcgcc cctgtccggg gagccacacc tcgatggcgt acttctgggc ggccgaggcg
     1681 cccagctccc cggtgcagac gctcacgacg cggtacggga ggccgagcgc tccgacgatc
     1741 tcctcctcgc acgagaggag gaactcgtgc tcgtcccacg aggcatccgg ctccgtgaac
     1801 gagaacatct cgaccttgtc gaactgatgc acgcggaaga tgccgcgggt gtccttcccg
     1861 tacgctccgg cctcgcggcg gaagcacgtc gagtacccca catagcgacg tgggagcgtc
     1921 gtcgggtcga ggatctcggt ctggtgcagc cccgctagcg gcacctcgga cgtccccacg
     1981 aggtacaggt cgtcgtcgcg ggtcgcgtag atctgcgcct cgtcggtcgg gaagaacccg
     2041 gtcccgtaca tcgcctcctc gcgaacgagg accggcggga cgatcggaag aaaccccttc
     2101 gcgatcaccc gatcgaggca gaagcgcacg agcgcgaact gcaagaggac cgcgcctccg
     2161 gcgaggtacg cgaaccgcgc accggagact cgggccgcac gctcgaggtc gatgatcccg
     2221 agcgcctgcc cgaggtcgac gtggtcccgc ggctcgaacg agaacgacgt cggcgccccg
     2281 acctcgcgga ggacgtcgtt gtcctcgtcg atctcgccgc ggggcaccga gggatgcggg
     2341 acgttcggga ggcgcgagag gacgatccgg agctcctcgt cgatcgcggc gaggcgcggc
     2401 tcgagctcct cgatgcgcgt cgagacgtct cggaccgacg cgatcaaggc gccgcggtct
     2461 tcgggcgctg cggcgccgat cgccttcgag gcacggttct gctccgcgcg gagcgactcg
     2521 acgctcgcgg tcagacgccg ccgttcggag tcgaggtcga cgacgcggtc gaggtcatcg
     2581 gccaaagaag gggcacgtcg cgcgagaccg tcgcggaacg actcggggtc gtcgcggatc
     2641 gctctgaggt cgagcaacta cgtccgcgag gtgattcggc gcccgcgccg cggcgggacc
     2701 tcgacgggcg cgccgagctc ccgtcgtgac gccagcgcct gtcgcaccgc ctcttcctct
     2761 tccgcggaca ggttgtagcg cgacgtcccg cgctcgacgg gcttcgcgta gcaccgcgtg
     2821 tcctgccgcc cgttgatcga cgtgacgacg aggccgtctc cctgctcgtc gagcagcgcc
     2881 gcggagaacg acaggtgtcc gcccatgtcc tcgaaggcgt cgtaccgcac gagcccgacg
     2941 cggccgatcg tgacgaggag ctgctccgcc tgccgccgct gctcggcctg cagcccccgg
     3001 aacacccgct tgagctcgtc gatcgcgtgc tcgtgcgccg cgagcgcctc gccgggggtg
     3061 gccgcccgcg gccggcggcc cgtgcgtctc ggcgccgagg cgaccacgag gaacacagcg
     3121 agcgccgcca ggccgaggct cacgaccacg aggatcgaga gcgtctgagg ggagaggacc
     3181 atcgagcgac actgtatggg gcggccaggg tcgccgcaat gaaccggtgc gcgttaggcc
     3241 gtcgcgcgca cgggggggtc gcagacgcga cacggctcga ggcgccgcgc ggcctggctt
     3301 cccgccttca tcctgcggac gtccttcccc gcgacgagga ggcagtcggg ccggtggaac
     3361 catcgtccgc
//
LOCUS       DASZOB010000173         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_113748, whole genome shotgun sequence.
ACCESSION   DASZOB010000173 DASZOB010000000
VERSION     DASZOB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113748"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VG709_02830"
     CDS             complement(<1..522)
                     /locus_tag="VG709_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32042.1"
                     /translation="MGGSGAENVRKGPDGARLRVGFRDTLSLVRRNRSFARLYSAQLV
                     SFAGDWFATVALVGLVEDLTGSATLVTLALVIQMASIAVLSPLGGLLADRLDRKRLMI
                     GADVVRVALALCYLLVDRPSEVWLAFAITAGISFLGAVFEPASSAAVPNIVDREDLPA
                     ANVLVGAAWGTMLA"
     gene            600..1247
                     /locus_tag="VG709_02835"
     CDS             600..1247
                     /locus_tag="VG709_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC32043.1"
                     /translation="MLERFTWFWQSAYRWSADGLTVYLDPRNVPEGYDPADAIFITHA
                     HFDHFNQDDIELLRKEDTVIVAPRDVASELSGDVVAVAPGDDVDVKGVKGQAVPAYNI
                     APDRLDKHPKENGWVGYMLSLGDLTIYHAGDTDHLPELEAIDARLAFVPIGGTFTMDA
                     REAAEFTKALSPEIAVPMHYGFVEGCGGPDAAETYRELAQPVRVDILDPQQPFEL"
     gene            1244..1549
                     /locus_tag="VG709_02840"
     CDS             1244..1549
                     /locus_tag="VG709_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32044.1"
                     /translation="MTRRTASLASATAWLLAAGLAVGLIAGHWAAGSGQSACASGTAG
                     LACLERTQTLAVRVGLFSGVMTMIVLLSYAGMLRMTVLDERRRATAAREARERAPLA"
     gene            1616..2530
                     /locus_tag="VG709_02845"
     CDS             1616..2530
                     /locus_tag="VG709_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HWC32045.1"
                     /translation="MALGYGGNLFILAFDHRGSFQKKMLGITGSPTPEERDRISDAKR
                     VIFEGFLHAVEAGAPRDAAGMLVDEEFGGDLARQAKKEDVIFAMPVEKSGQDVFDFEY
                     GEDFGQHIEDFDPTFTKVLVRYNPEGDRELNANQLVRLHRLSEWLHERDRKFLCEILV
                     PATPEQLERAGGDEDRYDNEIRPGLMVEVVRDFHEQGVEPDVWKIEGIDQRDDCRTIA
                     DTVRSEGRDDVACVVLGRGANEDAVVHWLQQGAGVPGYIGFAIGRTIWWDALKAYLDG
                     DDSRERAAERISENYRRMIDVYRRTDTP"
     gene            2542..2745
                     /locus_tag="VG709_02850"
     CDS             2542..2745
                     /locus_tag="VG709_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWC32046.1"
                     /translation="MAVIKTIDLVGVSADSWRDAAQQALAEASKTLRGIEGMEVLETS
                     AVVENNEIKEYQTHVRIRFRIER"
     gene            2771..>3508
                     /locus_tag="VG709_02855"
     CDS             2771..>3508
                     /locus_tag="VG709_02855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWC32047.1"
                     /translation="MPATILVGTQWGDEGKGKATDLLADHMDYVVRYQGGNNAGHTVR
                     AGGQTLKLHLIPSGILYDHITSVVADGVVVDPRVLLEEMDEIAARGIDTSRLLVSGNA
                     HLIMPYHLEIDRVTERYLGKNRLGTTKRGIGPAYADKAARIGLRVQDLLDEKIFRQKL
                     EVVLKEKNAILSRIYNRLPLDADAIVRDYRGYGERLAPHIADTSALLWRALRDGKRVL
                     LEGAQGTLLDLDHGTYPFVTSSNPLPRR"
BASE COUNT          589 a         1204 c         1232 g          483 t
ORIGIN      
        1 ggcgagcatc gttccccacg cggctccgac caggacgttg gcggccggca ggtcctcgcg
       61 atcgacgatg ttcggcaccg cggccgagga cgccggctcg aacaccgcgc cgaggaacga
      121 gatcccggcc gtgatcgcga acgcgagcca cacctccgac ggccggtcga cgagcaggta
      181 gcagagcgcc agggccaccc ggacgacgtc ggcgccgatc atgagccgct tccggtcgag
      241 gcggtccgcc aggagcccgc cgagcggcga caggaccgcg atcgaggcca tctggatcac
      301 gagcgcaagc gtcacgaggg ttgccgagcc cgtcaggtcc tcgacgagcc cgaccagcgc
      361 gacggtcgcg aaccagtcgc cggcgaacga caccagctgc gcggaataga gcctggcgaa
      421 gctgcggttt cgacgcacga gcgaaagcgt gtcgcggaaa cccacgcgga ggcgggcgcc
      481 gtccggcccc ttgcgaacgt tctcagcccc gctcccgccc acgcgcggag gctagcagcg
      541 gctaccgccg gcgccggaaa ggcggtagat atggccgggg gcgggtaggg aggatggcga
      601 tgctcgagcg gttcacgtgg ttctggcagt cggcgtaccg gtggtcggcc gacgggctga
      661 ccgtgtacct cgacccgagg aacgtccccg agggctatga ccccgccgac gcgatcttca
      721 tcacgcacgc gcacttcgac cacttcaacc aggacgacat cgagctgctc cgcaaggagg
      781 acacggtgat cgtcgcgccg cgcgacgtcg cgtcggagct ctcgggcgac gtggtcgcgg
      841 tcgcgccggg agacgacgtc gacgtcaagg gcgtgaaggg gcaggctgtc cccgcctaca
      901 acatcgcccc ggaccggctc gacaagcatc cgaaggagaa cggctgggtc gggtacatgc
      961 tctccctggg ggacctcacg atctaccacg cgggtgacac cgatcacctg cccgagctcg
     1021 aggcgatcga cgcgcggctc gcgttcgtgc cgatcggcgg gacgttcacg atggacgcgc
     1081 gggaagcggc cgagttcacg aaggcgctgt ctccggagat cgcggtcccg atgcactacg
     1141 gattcgtcga aggctgcggc ggtcccgacg ccgccgagac gtaccgggaa ctcgcgcagc
     1201 ccgtgcgcgt cgacatcctc gatccccagc agccgttcga gctgtgacgc gccgcaccgc
     1261 ctccctggcc tccgccacgg cgtggctcct ggcggccggg ctggccgtcg ggttgatcgc
     1321 cgggcactgg gccgcgggct cgggccagtc cgcgtgtgcg tccgggaccg ccgggctcgc
     1381 ctgcctcgag cgcacgcaga cgctggccgt ccgggtcggg ctcttctccg gcgtcatgac
     1441 gatgatcgtg ctcctctcgt atgcggggat gctgcggatg acggtcctcg acgagcggcg
     1501 gcgtgccacg gcggcgcgag aggcgcgcga gcgagcgccg ctcgcatagg gaggttggcc
     1561 cgccctgcgg gcctctgcga gaatggcgcg cgaaccggcc aaggagggtc gtgacgtggc
     1621 tcttggatac ggcgggaacc tgttcatcct cgcgttcgac catcgcggat cgttccagaa
     1681 gaagatgctc gggatcaccg gctcgccgac tccggaggag cgggaccgga tctccgacgc
     1741 gaagcgcgtg atcttcgagg gcttcctcca cgcggtcgag gcgggcgctc cccgtgacgc
     1801 cgcggggatg ctggtcgacg aggagttcgg cggcgatctc gccaggcagg cgaagaagga
     1861 ggacgtcatc ttcgctatgc cggtcgagaa gagcgggcag gatgtgttcg acttcgagta
     1921 cggcgaggac ttcggccagc acatcgagga cttcgacccg acgttcacga aggtactggt
     1981 ccgctacaac ccggaaggcg accgcgagct caacgcgaac cagctcgtgc ggctgcaccg
     2041 tctgtccgaa tggctccacg agcgcgaccg caagttcctc tgcgagatcc tcgtcccggc
     2101 tacgcccgag cagctcgagc gcgccggcgg cgacgaggac cggtacgaca acgagatccg
     2161 gcccggcctc atggtcgagg tcgtgcgcga cttccacgag cagggcgtcg agccggacgt
     2221 ctggaagatc gaggggatcg accagcgcga cgattgccga acgatcgccg acaccgtgcg
     2281 ctcggagggt cgtgacgacg tcgcgtgcgt cgtcctcgga cggggcgcca acgaggacgc
     2341 ggtcgtgcac tggctgcagc agggagcggg cgtccccggc tacatcgggt tcgccatcgg
     2401 gcgcacgatc tggtgggacg cgctcaaggc gtacctcgac ggcgacgatt cgcgggagcg
     2461 cgccgccgag cggatctccg agaactaccg acgcatgatc gacgtctatc gccggaccga
     2521 cacgccgtag agggggagca catggccgtc atcaagacca tcgatctcgt cggcgtgtcc
     2581 gccgactcgt ggcgggacgc cgcgcagcag gcgctcgccg aggcgtccaa gacgctgcgc
     2641 ggcatcgagg gcatggaggt cctcgagacc tccgccgtcg tcgagaacaa cgagatcaag
     2701 gagtaccaga cccacgtccg gatccgcttc cggatcgagc gctagccccg tctccggcct
     2761 cgactgagcg atgccggcga cgatcctggt cggcacccag tggggcgacg aggggaaggg
     2821 caaggccacc gacctcctcg ccgaccacat ggactacgtc gtccgctacc agggcggcaa
     2881 caacgccggc cataccgtcc gcgccggcgg gcagactctc aagctgcacc tgatcccctc
     2941 ggggatcctc tacgaccaca tcacgtccgt cgtcgccgac ggcgtcgtgg tcgacccgcg
     3001 cgtgctcctg gaggagatgg acgagatcgc cgcgcgcggc atcgacacgt cgcggctgct
     3061 ggtctcggga aacgctcacc tgatcatgcc gtaccacctc gagatcgaca gggtgaccga
     3121 gcggtacctc ggcaagaacc ggcttgggac gacgaagcgc ggcatcggac ccgcgtacgc
     3181 cgacaaggcc gcgcgcatcg gtctgcgcgt ccaggacctc ctggacgaga agatcttccg
     3241 ccagaagctc gaggtcgtcc tcaaggagaa gaacgcgatc ctgtcccgca tctacaaccg
     3301 gctcccgctg gacgccgacg cgatcgtccg cgactaccgg ggctacggcg agcggctggc
     3361 tccgcacatc gccgacacct cggccctgtt gtggcgggcg ctccgcgacg ggaaacgcgt
     3421 gctgctggag ggggcgcagg ggacgctgct cgacctcgat cacgggacgt atccgttcgt
     3481 cacgtcgtcg aaccccctcc cccgccgc
//
LOCUS       DASZOB010000174         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_94572, whole genome shotgun sequence.
ACCESSION   DASZOB010000174 DASZOB010000000
VERSION     DASZOB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94572"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VG709_02860"
     CDS             complement(<1..562)
                     /locus_tag="VG709_02860"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWC32048.1"
                     /translation="MADPRVARLADVVVSYSTGVRPGDLVVIDSTPLGAPVVRETYRR
                     VLAAGGHPEVRIAVDGVAEALLANGSDAQVEWVSPARTEEIERADVRIRFESDANTRA
                     LSGVDPARQALLHRARKPLGDRLMQRAAAGELRWLVTVFPTHAAAQDAGMSLVEYENF
                     VFRAGFLDREDPVSEWRAFGDRLARLA"
     gene            complement(555..>1570)
                     /locus_tag="VG709_02865"
     CDS             complement(555..>1570)
                     /locus_tag="VG709_02865"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWC32049.1"
                     /translation="RAALAAGANPYTNLTLDGLAELLVAEGSEDQIRYISPVDWHEVE
                     TVDAVVTIWSETNTRSFTCADAGRHAALISTRRRLSNRRWERIAAGEMLWCGTLFPTH
                     AHAQEAEMSLGDYEDFYFGACHADVDDPASHWRETARSLEARADELAAVRELRIVGPD
                     TDLTVGVEGRTWLAADGHFNMPDGEVFTSPVETATRGEIRYTYPALFQGREVDDVRLR
                     FENGEVVAAEASTGGAYLDELLDLARGARILGEVAFGGNYEIDRFTRNILLDEKIGGT
                     MHLALGSAFPQAGGSNTSALHWDMICDLREGGEVYADGELVWRSGEFLEEPAGLAAAG
                     PGG"
BASE COUNT          221 a          563 c          568 g          218 t
ORIGIN      
        1 cggcgaggcg cgcgagccgg tcgccgaagg cgcgccactc gctcacggga tcctcgcgat
       61 cgaggaaccc ggcccggaag acgaagttct cgtactcgac gagtgacatc cccgcgtcct
      121 gcgcggcggc gtgcgtcggg aagacggtga cgagccagcg gagctcgcct gccgccgccc
      181 gctgcatcaa tcggtcgccc agcggcttgc gcgcgcggtg gagcaacgcc tgccgcgcgg
      241 gatcgacgcc ggagagtgcg cgcgtgttcg cgtccgactc gaaccggatg cggacgtcgg
      301 cgcgctcgat ctcctcagtc cgcgcggggc tcacccattc gacctgagca tcgcttccgt
      361 ttgcgagcag ggcctcggcg accccgtcga cggcgatccg cacctcggga tgcccaccgg
      421 ccgcgagcac gcgccggtac gtctcgcgga cgaccggcgc gccgagcggc gtcgagtcga
      481 tgacgaccag gtcgcccggc cggacgcccg tcgagtacga gacgacgacg tccgcgagcc
      541 gcgcgacgcg cgggtcagcc acccggaccc gcggctgcga ggccggccgg ctcctcgagg
      601 aactctcccg agcgccagac gagctcgccg tcggcgtaca cctcgccgcc ctcccgaagg
      661 tcgcagatca tgtcccagtg gagggcggac gtgttcgagc cgcccgcctg cggaaacgcg
      721 gagccgagcg cgaggtgcat cgtcccgccg atcttctcgt cgagcaggat gttgcgcgtg
      781 aagcggtcga tctcgtagtt gcccccgaag gcgacctcgc cgagtatccg cgctccccgg
      841 gcgagatcga ggagctcgtc gaggtatgca ccccccgtcg aggcctccgc ggccacgacc
      901 tcgccgttct cgaagcggag gcgcacgtcg tccacctcgc gcccctggaa gagcgccggg
      961 tacgtgtagc ggatctcgcc gcgagtcgcc gtctcgacgg ggctcgtgaa gacctcgccg
     1021 tcgggcatgt tgaagtggcc gtccgcggcg agccacgtcc gcccctcgac gccgacggtg
     1081 aggtcggtgt cgggcccgac gatccggagc tcccggacgg cggcgagctc gtcggcccgc
     1141 gcttcgagtg accgagcggt ctcccgccag tgggaggcgg ggtcgtcgac gtcggcgtgg
     1201 caggcgccga agtagaagtc ctcgtagtcg ccgagggaca tctcggcctc ctgtgcgtgc
     1261 gcgtgcgtgg ggaagagcgt cccgcaccag agcatctcgc cggccgcgat ccgctcccag
     1321 cggcggttcg agagccggcg tcgcgtcgag atgagcgcgg cgtgacgacc ggcgtcggcg
     1381 caggtgaagg agcgcgtgtt cgtctccgac catatggtga cgacggcgtc gaccgtctcg
     1441 acctcgtgcc agtcgaccgg cgagatgtac ctgatctgat cctcggaccc ctcggcgacc
     1501 aggagctccg ccaggccgtc gagggtcagg ttcgtgtagg gattcgcgcc cgccgcgagc
     1561 gcggcccgga
//
LOCUS       DASZOB010000175         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_23990, whole genome shotgun sequence.
ACCESSION   DASZOB010000175 DASZOB010000000
VERSION     DASZOB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23990"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1268)
                     /locus_tag="VG709_02870"
     CDS             complement(21..1268)
                     /locus_tag="VG709_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWC32050.1"
                     /translation="MAEARIAGSSPASPSELGFDPWSVEFVAHPYGVYAELRELGPIH
                     YFEPTNQWIVPRCEDVNRLLRDRRFGRTYLHLATHDEMGRPAEPDWHRPFWHLIRSGI
                     LDMEPPQHTRVRRLVAKAFTPRRVESLRGYVQGVMDSLVDRVAGAGEFDLIRELAEPL
                     PVTVIAELLGVPESDRYLLRPWSAEICRMYELNPTDDDAWAAVTASVEFSDYLRELSR
                     ERRARPRDDLISALAQVLDEGERLTEDELIGTCVLLLNAGHEATVNVSGNGWWALFRN
                     PHKLEQLRADHSLVPTAVDELMRYDTPLQMFERWVLEDVDIHGVPVPKGSELALLFGS
                     ANRDPAVFDGPDELDLARDPNPHMSFGAGIHYCLGAPLGRLELQVSVGTLLRRLPSME
                     LVREPDWKPNYIIRGLRELIVRA"
     gene            complement(1346..>2238)
                     /locus_tag="VG709_02875"
     CDS             complement(1346..>2238)
                     /locus_tag="VG709_02875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HWC32051.1"
                     /translation="YGSNPEALVVILEGLGEAAVEWAASQPWIDFISGSYGDSLARPC
                     NDDGPNAVCGSREYRHTGPFVLRDGRTALFSAGNGASRTGLAYDRYSSLRPTSGPSWV
                     VTVGAASPRNGQDYGWHSVPVDVSSYGNHWPAAAAFTTNGERVFSGTSNATPVTAGVF
                     SKALLESRRALKDRREGIHELRDGTRVPAAGRRGPGMLADGVLTRLELQDAVFKTAFP
                     EAFDAERWTYDPTVVPDSPAYYTQEGYGIANVASGLRAIDVIFGRAPMPDRAEVDAWI
                     ARIDGVRDMVYPPDRYDGIE"
BASE COUNT          329 a          801 c          787 g          321 t
ORIGIN      
        1 gtcggcttcg ggcgacgggc tcacgcccgc acgatcagct cgcgcagccc gcggatgatg
       61 tagttcggct tccagtcggg ctcgcggacg agctccatgc tgggcagccg ccggagcagc
      121 gtgccgaccg agacctgcag ctcgagacgc ccgagcggag cgccgaggca gtagtggatc
      181 cccgcgccga acgacatgtg cgggttggga tcgcgcgcga ggtcgagctc gtccggcccg
      241 tcgaacaccg ccgggtcgcg gttcgccgag ccgaacagca gcgcgagctc cgagcccttc
      301 gggaccggga cgccgtggat gtcgacgtcc tccaggaccc atcgttcgaa catctgcagc
      361 ggcgtgtcgt agcgcatcag ctcgtccacg gccgtcggga cgagcgagtg atcggcgcgc
      421 agctgctcga gcttgtgagg gttccggaac agcgcccacc agccgttgcc ggagacgttc
      481 accgtcgcct cgtggcccgc gttgaggagc agcacgcacg tcccgatcag ctcgtcctcc
      541 gtgagacgct ccccctcgtc caaaacctgc gcgagcgcgc tgatcaggtc gtcgcgcgga
      601 cgcgcgcgtc gctcccggga gagctctcgg aggtagtcgg agaactcgac gctcgccgtg
      661 acggcggccc atgcatcgtc gtcggtgggg ttcagctcgt acatgcggca gatctccgcc
      721 gaccacggcc gcagcaggta tcggtccgac tcggggacgc ccaggagctc cgcgatgacg
      781 gtgaccggca gcggctccgc gagctcccgg atgaggtcga actcgccggc gccggcgacc
      841 cggtcgacga gcgagtccat gactccctgg acgtaccccc gcagcgactc gacgcgccga
      901 ggcgtgaacg ccttcgcgac gagacgcctc acccgcgtgt gctgcggcgg ctccatgtcg
      961 aggatgccgg agcggatcag gtgccagaaa ggccggtgcc agtccggctc cgcagggcgg
     1021 cccatctcgt cgtgcgtcgc caggtggagg tacgtcctgc cgaaacggcg gtcgcgcagc
     1081 aggcggttca cgtcctcgca gcgcgggacg atccactggt tcgtcggctc gaagtagtgg
     1141 atcggcccga gctctcgcag ctcggcgtac acaccgtacg ggtgcgcgac gaactcgacg
     1201 ctccacgggt cgaacccgag ctctgaaggg gacgccggcg aggatccggc gatccgagcc
     1261 tcagccatcc ccccgagcct accccttcgc ggaaacctca cgggtcgcga cgctcgcgcg
     1321 ccgcgacccg tttcggcgtg cgacgctact cgatgccgtc gtaccggtcg ggcgggtaca
     1381 ccatgtcgcg gacgccgtcg atccgcgcga tccacgcgtc gacctcggcg cgatccggca
     1441 tcggagcccg cccgaagatc acgtcgatcg cccgtagacc cgaggcgacg ttcgcgatcc
     1501 cgtacccctc ctgcgtgtag tacgcgggcg agtcggggac gaccgtcggg tcgtacgtcc
     1561 acctctcggc atcgaacgcc tcagggaacg ccgtcttgaa gaccgcatcc tggagctcca
     1621 gccgtgtgag gacgccgtcg gcgagcatgc cgggcccgcg tcgtcccgcg gcgggcacgc
     1681 gcgtcccgtc ccggagctcg tggatcccct cgcgtcggtc cttcagggcg cggcgggatt
     1741 cgagcagcgc cttcgagaac acgccggccg tcaccggcgt cgcgttgctc gtgccgctga
     1801 acacgcgctc gccgttcgtc gtgaacgcgg ccgccgccgg ccagtgattg ccgtacgagg
     1861 acacgtcgac cggcaccgag tgccagccgt agtcctggcc gttgcgcggg ctcgcggctc
     1921 caaccgtgac gacccacgac ggcccggagg tcgggcgcag gctcgagtac cggtcgtagg
     1981 cgagtcccgt gcgagacgcc ccgttgccgg ccgagaacag ggcggtgcgc ccgtcgcgga
     2041 gcacgaaggg cccggtgtgg cggtactcgc ggctgccgca gacggcgttc gggccgtcgt
     2101 cgttgcacgg tcgagccagc gagtcgccgt acgagcccga gatgaagtcg atccatggct
     2161 ggctcgcggc ccactcgacg gcggcctcgc cgagcccctc gaggatcacg acgagcgcct
     2221 ccgggttcga gccgtagg
//
LOCUS       DASZOB010000176         6720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_165339, whole genome shotgun sequence.
ACCESSION   DASZOB010000176 DASZOB010000000
VERSION     DASZOB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165339"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VG709_02880"
     CDS             <1..424
                     /locus_tag="VG709_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017065264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HWC32052.1"
                     /translation="VYSDALPEGERIVEREGTPGVARVVYSRGAYADGVEAVHPVRRR
                     VVRAPVDRLVVVGSAPVDAVEGVASWYERDGLVAAHPSLPIGTRVRVTGVASGRSVTV
                     IIDDRGPYAGGRIIDLSDDAFSRIAPLSAGTAVVRIAW"
     gene            447..1250
                     /gene="rsmA"
                     /locus_tag="VG709_02885"
     CDS             447..1250
                     /gene="rsmA"
                     /locus_tag="VG709_02885"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017195521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWC32053.1"
                     /translation="MEIAERHGIRPRKSLGQHFLADVNLAEAIAREAGAAPGTRFLEI
                     GPGLGSLTVALADAGAEVLAVEIDRRVADALGESIGSRPGVSVVVADALRVDWPSVLD
                     DGAWRMASNLPYNVAVPVVMELLEQAPGVDPMLVMVQREVGERLAAAPGTASYGAVSV
                     RVSYHASVRLVRRVGTKVFWPEPNVESVVVRLDRREPPVRGARERVMRLVDEGFAQRR
                     KTLASALVRLGMDRVAARAALEGAGIEASSRAEQLSIEDFGRLAEAVDG"
     gene            1243..2112
                     /gene="ispE"
                     /locus_tag="VG709_02890"
     CDS             1243..2112
                     /gene="ispE"
                     /locus_tag="VG709_02890"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWC32054.1"
                     /translation="MAEPGRVVSEPAPAKLNPFLRVLGRRSDGFHDIETLGQPVTLAD
                     GVRAETADAGLLSLSIAGEQREAVPRGDDNLVLRAARELLVEVGPASGARLLLVKRIP
                     VAAGLGGGSADAAAALRALNDLWGVGLTSDALADIGARVGSDVPALVHVAPVIARGRG
                     EDVEPVDLPRTWWIVLPASGVSAAESYAAWDEDARDVGPDVAPLLEAMRSGDLDAVAG
                     YLFNDLEAPVAERHAEVARRRQALLEAGAIGAIMCGSGPSVAGLALDGTDAERIANAV
                     GGIAVSAIGGRPA"
     gene            complement(2115..2185)
                     /locus_tag="VG709_02895"
     tRNA            complement(2115..2185)
                     /locus_tag="VG709_02895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Gln,seq:ttg)
     gene            complement(2399..3598)
                     /locus_tag="VG709_02900"
     CDS             complement(2399..3598)
                     /locus_tag="VG709_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWC32055.1"
                     /translation="MHRAVAAALLLLTACTGAAPARRATGPSVGSIPSPARVEQTSAP
                     RATTADRPELDAWLLWTLGGIPPGLRETVRSLGEIRDVAQVASGTTWLLASGDEPVRG
                     DESRFAIPFEVAAVRKGEYARFLPPRAARAVRGLGQLDAVLGSTGAALLDVEVGGIAL
                     REGNLSARGVLADGLIGEHEALVSMPTGRSLGVNAVRYLLVEPEEGVPPDRMHRALMS
                     AVPPGVPARVRAPGSTAYVRHADLTLPQSRLKALFGEFAAAPAGGWFEVERSWVRRNI
                     VEARVPILGRVTCHRRIVPLVRGALRELVRRGLGRLVDPAQYGGCYAPRFIGRDPGAG
                     LSHHAWGVAIDINVADNPLGAEPTMDRRVVRSFERWGFVWGGDFIRPDGHHFEFRCFP
                     SRCAEPH"
     gene            3740..5128
                     /gene="glmU"
                     /locus_tag="VG709_02905"
     CDS             3740..5128
                     /gene="glmU"
                     /locus_tag="VG709_02905"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWC32056.1"
                     /translation="MAAGKGKRLKSRTPKVLHEVCGRPVLWHVLTALRAVRPDPVIVV
                     INHDGDTIRAAVESWGLRLPVKFVDQGEALGTGHAVMVAERAASGSDEVLVLPGDEPL
                     VTGQQLRELLTMHRRRDVAAVIQTTIAGNPRGFGRIIRDDNDLVRITEGDEASSAELK
                     IPEVATSVYAFRREDLFKALPLLSRETKQREYYLHHVLSILQEKGERVAVQRVDNGGA
                     VGANTRSELASAAAVMRRRINEEHMANGVTLVDPDRTYIDAGVAIGPDTVVYPMTFLT
                     GTTRVGERAVVGPGARIEDSRIDDQAVVEQSSVRGARIGAGATVGPYAHIRPGTVLGR
                     GAKAGSFVEIKGSRIGDGSKVPHLAYVGDAVIGRNVNVGAGTITCNYDGIRKHRTVIS
                     DDAFIGSDTMLVAPVRIGRGAVTGAGSTITRDVPAGALAVERAEQRTVRGYAARKAAE
                     KERKPRKRRGKA"
     gene            5139..6119
                     /locus_tag="VG709_02910"
     CDS             5139..6119
                     /locus_tag="VG709_02910"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWC32057.1"
                     /translation="MREIVTKKRLMLFAGTAHPQLAEEIAEALGVPLAKARLSRFASG
                     EIYFRSDESVRGVDCFVIQTHADPVNESIMEQAIMIDALKRASAKRITAVVPYYAYSR
                     QDKKSLSREPISGKLVADILSAAGADRVVSVDLHSGQIQGFFDFPFDHLTGLPLLADY
                     LRNDLELRDDLVVVAPDTGRVKVAERLGDYLRADLAFIYKRRSRREAHKIEEMIVEGD
                     VEGRACLLIDDMIDTGGTVAEAARTLVREGGGDIYAAATHAVLSGKAVQRLEEAPIKR
                     VIFTNTLPIPEERRIEKMTVLSISPIIASAVEAVFEDTSVSEIFHGENQP"
     gene            6178..>6720
                     /locus_tag="VG709_02915"
     CDS             6178..>6720
                     /locus_tag="VG709_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWC32058.1"
                     /translation="MEVRLKAEKRDGAGKGVARKIRATGNVPAILYGPTQEAFPLQVR
                     ERDLWHALHTDAGTNVLVNLAVDGDTFLAMPREVQRDIVRGTLLHVDFLQIRRDVEIQ
                     VDVPIQLVGDSQGVQEGGVVEHHLWEVRVECLPTNVPESIDADITNLGVNDALHVSDI
                     TAPDGVKILTPPEEVVVTVVP"
BASE COUNT         1023 a         2327 c         2429 g          941 t
ORIGIN      
        1 cgtctactcg gacgcgctgc ccgaagggga gcggatcgtc gagcgcgagg gcacgccggg
       61 cgtcgcgcgc gtcgtgtact cgcgtggcgc gtacgccgac ggcgtcgagg cggtgcaccc
      121 cgtccgccgc cgcgtcgtac gggcgcccgt ggatcgcttg gtggtcgtcg ggagcgcgcc
      181 ggtcgacgcc gtcgagggtg tcgcgagctg gtacgagcgc gacggcctcg tcgccgctca
      241 cccgtcgctc ccgatcggga cgcgcgtgcg agtgaccggc gtcgcgagcg ggcgaagcgt
      301 caccgtgata atcgatgacc gtgggccgta cgccggcgga cggatcatcg acctctccga
      361 cgacgcgttc tcgcggatcg caccgctctc cgccggaacg gcggtcgtcc gcatcgcctg
      421 gtagccggct cacgcgccgc cgcctcgtgg agatcgccga gcgccacggc atccgtccga
      481 ggaagtcgct ggggcagcac ttcctcgccg acgtgaacct cgcggaggcg atcgcccgcg
      541 aggcaggcgc ggcgcccggg acgcgcttcc tcgagatcgg gcccggcctc gggtcgctga
      601 cggtggcgct cgccgacgcc ggcgcggagg tcctcgcggt cgagatcgac cgccgcgtcg
      661 ccgacgcgct cggagagtcc atcggctcga gaccgggggt gagcgtcgtc gtcgccgacg
      721 cactccgcgt cgactggccg tcggtgctgg acgacggcgc gtggcggatg gcgtcgaacc
      781 ttccgtacaa cgtcgcggtg cccgtggtga tggagctgct ggagcaggcc cccggcgtcg
      841 atccgatgct cgtgatggtc caacgcgagg tcggagagcg cctcgctgcg gctcccggga
      901 ccgcgtccta cggcgcggtg tcggttcgcg tctcgtacca cgcgagcgtg cgcctcgtac
      961 gccgcgtcgg gaccaaggtt ttctggccgg agccgaacgt cgagtcggtc gtcgtgcggc
     1021 tcgaccgtcg cgagccaccg gtgcgaggag ctcgcgagcg cgtcatgagg ctcgtcgacg
     1081 aggggttcgc gcagcggcgg aagacactcg cctcggcgct ggtgcggctc gggatggatc
     1141 gcgtggcggc gcgagcggcg ctcgagggcg ctgggatcga ggcgtcgtcg cgcgcggagc
     1201 agctctcgat cgaggacttc gggcgactcg ccgaggccgt cgatggctga gccggggcgc
     1261 gtcgtctccg agccggctcc ggccaagctg aatccgttcc tgcgggtgct cgggcggcgg
     1321 agcgacgggt tccacgacat cgagacgctc ggccagccgg tcacgctggc cgacggcgtc
     1381 cgcgccgaga cggccgacgc cgggctgctg tcgttgtcga tcgccggcga gcagcgtgag
     1441 gccgtcccgc gaggcgacga taacctcgtg ctccgcgcgg cacgtgagct gctcgtcgag
     1501 gtcggtcccg cgtcgggggc acggctgctg ctcgtgaagc ggatcccggt ggcggcgggc
     1561 ctcggcggcg gcagcgcgga cgccgccgcg gcgctgcgcg ccctgaacga cctgtgggga
     1621 gtcggcctca cgagcgacgc gctcgcggat atcggggcaa gggtcggctc agacgtgccc
     1681 gccctcgttc acgtcgcgcc cgtcatcgcg cgcggacgcg gcgaggacgt ggagcccgtc
     1741 gacctccccc ggacgtggtg gatagtgctt ccggcgtccg gggtgagcgc cgcggagtcg
     1801 tacgcggcct gggacgagga cgcgcgcgac gtcggaccgg acgtcgcgcc gctcctcgag
     1861 gccatgcgct caggcgacct cgacgccgtc gccggctacc tgttcaacga cctcgaggcg
     1921 ccggtcgcgg aacggcacgc ggaggtcgcg cgaaggcgtc aggcgctcct cgaggcgggc
     1981 gcgatcggcg cgatcatgtg cggcagcggc ccgagcgtcg cgggcctcgc actggacggg
     2041 accgatgccg aacggatcgc aaatgccgtc ggaggcatcg ctgtgtcggc gatcggcggt
     2101 cgacccgcgt gacgctccgg agggaggatt cgaacctcca cctgaggagc caaagtcctc
     2161 cgtgctgccc ttacaccact ccggaacggt tcaagtatgc gggaacctag gcgtggtgag
     2221 gccgagcaag tcgatgctag gagatccctg agcgctccgc cccggctctt cagagcgagc
     2281 tcttgagcga tctcgcgttt cgaattaccc gagaagacgg agctgccgag cgcggtctct
     2341 cttcggggcc gtggccgtca ccgttcgatg ctagcgatgg ccactgacat ccatccgttc
     2401 agtgcggttc ggcgcacctc gacgggaaac agcggaactc gaagtggtgg ccgtcggggc
     2461 ggatgaagtc gccgccccac acgaagcccc agcgctcgaa cgaccgcaca acacggcgat
     2521 ccatcgtcgg ctccgcgccg aggggattgt cagcgacgtt gatgtcgatc gcgactcccc
     2581 acgcgtggtg cgatagcccg gcgccgggat ctcggccgat gaaccgcggc gcgtagcagc
     2641 cgccgtactg ggcggggtcg acgagacgtc cgagccctcg ccgaacgagc tcgcgcagcg
     2701 cgccgcggac cagcgggacg atccgtcggt gacacgtcac ccggccgagg atcggaacgc
     2761 gtgcctcgac gatgttgcga cggacccagg agcgctcgac ctcgaaccat cctccggcag
     2821 gggccgcggc gaactcgccg aacagcgcct tcagtcgcga ctgcggaagc gtgaggtcgg
     2881 cgtgacgcac gtaagccgtg cttccgggcg cccttacccg cgccggcacg ccggggggga
     2941 cggcgctcat cagcgcgcgg tgcatgcggt ccggagggac gccctcctcc ggctcgacga
     3001 gcaggtagcg aacggcgttc accccgagcg accgaccggt cggcatggag acgagtgcct
     3061 cgtgctcgcc gatgaggccg tccgcgagaa cgccacgcgc cgagaggttc ccctcgcgca
     3121 gagcgatccc tccgacctcg acgtcgagaa gcgccgcgcc ggtgctcccg aggaccgcat
     3181 cgagctgtcc gaggccgcgt acggctcgag cggccctcgg cggtaggaac cgcgcatact
     3241 cgccctttcg cacggccgcg acctcgaacg ggatcgcgaa cctggactcg tctcctcgca
     3301 cgggctcgtc tcccgatgcg aggagccacg tcgtcccgct cgcgacctgc gcgacgtccc
     3361 ggatctcgcc gagggaccgg acggtctccc ggagaccggg cgggatccct ccgagcgtcc
     3421 agagcagcca cgcgtcgagc tccggccggt cggctgtggt cgcccggggc gcggacgtct
     3481 gctcgacgcg cgcgggcgac gggatcgacc cgacggacgg gccggtcgcc cgccgtgccg
     3541 gggccgcccc ggtgcacgcg gttagaagga gaagcgcggc ggcgacggcc ctgtgcacgg
     3601 tggttcgacg ttctccgccc gcgtcgggtt cagcggtctt gcgtatcggg gaggcggcgg
     3661 gtacaaaggc cgcgcacgag ggcacccccc aaggacgcgc atggccagga agccttcccg
     3721 aactctcgca gccgtcgtca tggccgccgg caagggcaag cggctcaagt cgcgcacgcc
     3781 caaggtgctc cacgaggtgt gcggcaggcc cgtcctctgg cacgtcctca ccgcgctccg
     3841 cgccgtccgc ccggaccccg tcatcgtcgt catcaaccac gacggcgaca cgatccgcgc
     3901 cgcggtcgag tcatgggggc tcaggcttcc ggtgaagttc gtggatcagg gtgaggcact
     3961 cggcacgggt cacgccgtca tggtcgccga gcgcgccgcg tctggatcgg acgaggtcct
     4021 cgtgcttccg ggcgacgagc cgctcgtaac cggccagcag ctgcgcgagc tgctcacgat
     4081 gcaccgccgc cgggacgtcg cggccgtcat ccagacgacg atcgcgggga acccgcgcgg
     4141 gttcggccgg atcatccgcg acgacaacga cctcgtcagg atcaccgagg gcgacgaggc
     4201 ctcgagcgcg gagctcaaga tcccggaggt ggcgacatcg gtctacgcgt tccggcgcga
     4261 ggacctcttc aaggcgctgc cgctgctctc gcgcgagacc aagcagcgcg agtactacct
     4321 ccatcacgtg ctctcgatcc tccaggagaa gggcgagcgc gtcgccgtgc agcgcgtcga
     4381 caacggcgga gccgtcggcg ccaacacgcg ctccgagctc gcatcggccg cggcggtcat
     4441 gcggcggcgc atcaacgagg aacacatggc gaacggcgtc acgctcgtcg atcctgaccg
     4501 cacgtacatc gacgcgggcg tcgcgatcgg acccgacacg gtcgtctacc cgatgacgtt
     4561 cctcaccggc acgacgcgcg tcggcgaacg cgcggtcgtc ggcccgggcg cgcggatcga
     4621 ggactcgagg atcgacgacc aagccgtcgt cgagcagtcg agcgtccgtg gcgcccgcat
     4681 cggcgccggc gcgaccgtcg gcccgtacgc gcacatccgt cccggtaccg tgctcggccg
     4741 cggcgccaag gccgggagct tcgtcgagat caagggatcc cggatcggcg acggcagcaa
     4801 ggttccccat ctcgcctacg tcggcgacgc cgtcatcggg cgcaacgtca acgtcggggc
     4861 cggaacgatc acgtgcaact acgacggcat caggaagcat cgaacggtga tctccgacga
     4921 cgcgttcatc ggctcggata cgatgctggt ggcgccggtt cgcatcggcc gaggagccgt
     4981 gacgggagca gggtcgacga tcacgcgcga cgtcccggcg ggcgcgcttg ccgtcgaacg
     5041 cgcggagcag cgcaccgtca ggggctatgc ggcacggaag gcggcggaga aggagcggaa
     5101 gccccggaag cgccggggga aggcctaggg ggagcgcgat gcgcgagatc gtcacgaaga
     5161 agcgcctgat gctgttcgcc gggacggcgc atccgcagct cgcggaagag atcgccgagg
     5221 cgctgggcgt ccccctcgcg aaggcacggc tttcgaggtt cgcgagcggc gagatctact
     5281 tccgttccga cgaaagcgtg cgcggcgtcg actgcttcgt gatccagacc cacgccgacc
     5341 ccgtgaacga atcgatcatg gagcaggcga tcatgatcga tgcgctgaag cgcgcgtccg
     5401 ccaagcggat cacggcggtc gtgccgtact acgcgtattc gcggcaggac aagaagtcgc
     5461 tttcgcgtga gccgatctcc ggcaagctcg tcgcggacat cctgtcggcc gccggtgccg
     5521 accgcgtcgt gtcggtcgac ctgcacagcg ggcagatcca ggggttcttc gacttcccgt
     5581 tcgaccacct gacggggctc ccgcttctcg ccgactatct ccgcaacgac ctcgagcttc
     5641 gcgacgatct cgtcgtcgtc gcccccgaca cggggcgcgt gaaggtggcc gagcggctcg
     5701 gggactacct tcgcgccgac ctcgcgttca tctacaagcg ccgttcgcgt cgcgaggcgc
     5761 acaagatcga ggagatgatc gtcgagggcg acgtcgaggg tcgcgcgtgc ctgctcatcg
     5821 acgacatgat cgatacgggt gggaccgtcg cggaggcagc caggaccctc gtgcgcgagg
     5881 gcggcggcga catctacgcg gcggcgaccc atgcggtgct gtcggggaag gcggttcagc
     5941 gcctcgagga ggcgccgatc aagcgggtga tcttcacgaa cacgctgccg atcccggagg
     6001 agcgcaggat cgagaagatg acggtgctct cgatctcgcc gatcatcgcc tcggccgtcg
     6061 aggcggtctt cgaggacacg agcgtatccg agatcttcca cggcgagaac cagccctaga
     6121 gcgacccgga agacctgccg gcggccgcta caatgactgg ccgaccggga ggttcacgtg
     6181 gaagtcaggc tgaaggcgga gaagcgagac ggcgccggca agggcgtcgc gcggaagatc
     6241 cgggcaaccg gcaacgtgcc ggcgatcctg tacgggccga cgcaggaggc gttcccgctc
     6301 caggtccgtg agcgcgacct gtggcacgcg ctccacaccg acgccgggac gaacgtcctc
     6361 gtgaacctcg ctgtcgacgg cgataccttc ctcgcgatgc cgcgtgaggt ccagcgcgac
     6421 atcgtccggg gcacgctgct ccacgtcgac ttcctacaga tccgtcgcga cgtcgagatc
     6481 caggtcgacg tcccgatcca gctcgtcggc gactcgcaag gcgtccagga gggcggcgtc
     6541 gtcgagcacc acctctggga ggtccgcgtc gagtgcctcc cgacgaacgt tcccgagagc
     6601 atcgacgccg acatcacgaa cctcggcgtg aacgacgccc tacacgtctc cgatatcacc
     6661 gcgcccgacg gcgtgaagat cctcacgccg cccgaggagg tcgtcgtgac cgtcgtccct
//
LOCUS       DASZOB010000177         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_144956, whole genome shotgun sequence.
ACCESSION   DASZOB010000177 DASZOB010000000
VERSION     DASZOB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144956"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /gene="rnpB"
                     /locus_tag="VG709_02920"
     ncRNA           <1..337
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VG709_02920"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            362..901
                     /locus_tag="VG709_02925"
     CDS             362..901
                     /locus_tag="VG709_02925"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWC32059.1"
                     /translation="MRSPQARLSVIGLVAVGVLASLWIVAARRPQETGREPVELSGRV
                     PSIDAPAVVGNDRVTPELYSGKTLVVNFWASWCGPCRREQPGLEALWREYRDRGVQFV
                     GVDFKDDAAAAREFVREFDVTYPSARDPSGAIGADFAIPYVPTTVLADADGELRWLLP
                     GEQHPEQLREYLEALLAEG"
     gene            complement(921..1409)
                     /locus_tag="VG709_02930"
     CDS             complement(921..1409)
                     /locus_tag="VG709_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32060.1"
                     /translation="MSRRVSITVFVFVLVLASCGRANAPSETAARERPPRAPATGHRA
                     WPSTEIGPAVSCVSEYPHRLDDRAYAFDGTIQEVTRGEYDQDAGATPVDLRLRVNEVF
                     QGDLGDVVTMHTWNFMLPEHDVRGTRILASAGPTLDLMGCGYTRPYSEEDAQYWRDVF
                     SA"
BASE COUNT          231 a          517 c          566 g          234 t
ORIGIN      
        1 agtccggact ccacagggcg cggcgctggg taacgcccag gcagggtgac ctgcggaaag
       61 tgccacagag aacagaccgc caacggcccc gacgacggct cgccgccgag gggtgcgggc
      121 aagggtgaaa cggtgaggta agagctcacc agcgcgccgg gcgaccggcg cggctcggca
      181 aaccccaccc ggagcaaggc caagcaggga gcgttcgagg gcggcccgtc cgagctcccg
      241 ggtcggccgc accgagcgtc gcggcaacgc ggcgcccaga gagatggtcg cccccgcgca
      301 agcgggacag aatccggctt acaggccggc tcctcacacc ccctggtacg ttggcctcgt
      361 gatgcgatcg cctcaggcac ggctctcggt catcgggctc gtcgccgtcg gcgtgctggc
      421 gtcgctgtgg atcgtcgcgg cgcggcgtcc tcaagagacc gggcgggagc ccgtggagct
      481 ctccggacgc gttccgtcga tcgacgcccc cgccgtcgtc ggcaacgatc gcgtcacgcc
      541 cgagctctac tcggggaaga ccctcgtcgt gaacttctgg gcgtcgtggt gcgggccgtg
      601 ccgacgcgag cagccggggc tcgaggcgct ctggcgcgag taccgggatc gcggtgtgca
      661 gttcgtcggc gtcgacttca aggacgacgc cgccgccgca cgggagttcg tccgcgagtt
      721 cgacgtcacg tatcccagcg ccagggaccc gagcggcgct atcggcgccg acttcgcgat
      781 cccgtacgtc ccgaccacgg tgctggcgga cgccgacggt gagctcaggt ggctgctgcc
      841 cggcgagcag catccggaac agctccgcga gtacctcgag gcgctgttgg ctgaaggctg
      901 atggaagtcg ttagaagtcg ttacgcgctg aagacgtccc gccagtactg agcgtcttcc
      961 tccgaatagg gccgcgtgta gccacacccc atcaggtcga gggttgggcc ggcggacgcg
     1021 aggatccgtg tccctctcac gtcgtgttcg ggaagcatga agttccacgt gtgcatcgtg
     1081 acgacgtcgc cgaggtcgcc ttggaacacc tcgttgaccc ggagccgcag atcgaccggc
     1141 gtggcgcccg catcctggtc gtactcgccg cgcgtcacct cctggatcgt cccgtcgaac
     1201 gcgtacgcgc ggtcgtcgag acggtgcggg tattcggata cgcagctgac ggccggcccg
     1261 atctcggtcg acggccatgc gcggtggccg gtggccggcg cccgtggcgg tcgctcgcgc
     1321 gcagccgtct ccgatggcgc gttcgcgcgt ccgcacgacg cgaggacgag gacgaagacg
     1381 aagacagtga tgctgacgcg ccgggacatc gcgtctcctc ggtcgactga gcgtttgacg
     1441 cgcgttcggg ttcgatggtt ccgacgcgcg actgccgggt gcgcttagcc ggcgagcgcg
     1501 ccggaggcga cggcggtgcg gtgttgcttg cctgcgtggt acgaggag
//
LOCUS       DASZOB010000178         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_155031, whole genome shotgun sequence.
ACCESSION   DASZOB010000178 DASZOB010000000
VERSION     DASZOB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155031"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VG709_02935"
     CDS             <1..623
                     /locus_tag="VG709_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWC32061.1"
                     /translation="YVSLQDWGRGVIHLAVQRAQLERDAELRSLAVSFGASLSRTEVE
                     SVLAEPGAHSEMLGVYFTDGDQHFDHRSLQDHVAPNCTSDLLYKGALKDASRVVYSGL
                     IRIHPGARRSDAYQTNRNVVLSDEAKADSIPNLEIENNDVRCSHAASVGPVDEDVRFY
                     LESRGIPRDEAEQLIVTGFFQEVLDRVPLEEVRGSLEAAIEDELRR"
     gene            628..942
                     /locus_tag="VG709_02940"
     CDS             628..942
                     /locus_tag="VG709_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWC32062.1"
                     /translation="MALVTIARTTDVPEGEARRFVVDGREIALVNLGEDGFRAVDDIC
                     SHAQAYLHEGEVDTDFETIECPRHGSTFDLETGKPRSLPATVPVAVYNVKVEGDEVKV
                     EI"
     gene            944..>1725
                     /gene="sufC"
                     /locus_tag="VG709_02945"
     CDS             944..>1725
                     /gene="sufC"
                     /locus_tag="VG709_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWC32063.1"
                     /translation="MTERDATLEIDDLHASVDGKDILRGVTLTVRQGETHALMGPNGS
                     GKSTLAGIIMGRPGYEITGGDVRYGGESGLRLSPDERARRGLFLAFQYPVEVPGVSVV
                     NFLRSAYNALHPAEGDGKGMSALAFRKLMKEKMSLVGVDDDMVRRYVNQGFSGGEKKR
                     METLQLAVLEPRIAILDETDSGLDIDALKAVANGIEQLRGPDLGLLLITHYQRILNYV
                     TPDHVHVLMAGRIVRSGGAELAHELEEKGYEGMRTEVGVVGGA"
BASE COUNT          288 a          564 c          631 g          242 t
ORIGIN      
        1 gttacgtgtc gctccaggac tgggggcgcg gagtcatcca cctcgcggtg cagcgcgcgc
       61 agctcgagcg cgacgcggag ctccggtcgc tggcggtgtc gttcggcgcg tcgctctcgc
      121 gcacggaggt cgagtcggtg ctcgccgagc ccggcgcgca cagcgagatg ctcggggtgt
      181 acttcaccga cggggaccag cacttcgatc accgctcgct gcaggaccac gtcgcgccga
      241 actgcacgag cgacctgctc tacaagggcg cactgaagga cgcgtcgcga gtggtctact
      301 cgggcctcat ccgcatccat cccggcgcgc gcaggagcga cgcgtaccag accaaccgca
      361 acgtcgtgct ctccgacgag gcgaaggccg actcgatccc gaacctcgag atcgagaaca
      421 acgacgtccg gtgctcccac gccgcgagcg tcgggcccgt cgacgaggac gtgcgcttct
      481 acctcgagag ccgagggatc ccgcgcgacg aggcggagca gctgatcgtg accgggttct
      541 tccaggaggt gctcgaccgc gtgccgctcg aggaggtccg cggctcgctc gaggccgcga
      601 tcgaggacga gctgcggagg tgacggcgtg gcgctcgtga cgatcgcgcg aacgaccgac
      661 gtgccggagg gggaggcccg ccggttcgtc gtcgacggac gggagatcgc gttggtcaac
      721 ctcggcgagg acgggttccg ggccgtcgac gacatctgct cgcacgccca ggcgtacctg
      781 cacgagggcg aggtggatac cgacttcgag acgatcgagt gcccgcggca cggctcgacg
      841 ttcgacctcg agaccggcaa gccgcggtcg ctcccggcca ccgtgccggt ggcggtctac
      901 aacgtgaagg tcgaaggcga cgaagtgaag gtggagatct gacatgacgg aacgagacgc
      961 gacgctcgag atcgacgacc tgcacgcgtc ggtcgacggc aaggacatcc tccgcggcgt
     1021 cacgctgacg gtgcggcagg gcgagacgca cgcgctgatg ggcccgaacg ggtccggcaa
     1081 gtcgacgctc gccgggatca tcatgggccg tcccggctac gagatcaccg gcggggacgt
     1141 ccgttacgga ggggagtccg gtctccggct atcgcccgac gagcgggcgc ggcgagggct
     1201 gttcctcgcg ttccagtacc cggtcgaggt ccccggcgtc tcggtcgtga acttcctgcg
     1261 ttccgcgtac aacgcgctgc acccggccga gggcgacggc aaggggatgt cagcgctcgc
     1321 gttccgcaag ctcatgaagg agaagatgtc gctcgtcggc gtcgacgacg acatggtgcg
     1381 ccggtacgtc aaccaggggt tctcgggcgg cgagaagaag cgcatggaga cgctccagct
     1441 cgcggtcctc gagccgagga tcgcgatcct cgacgagacg gactcgggcc tcgacatcga
     1501 cgcgctcaag gccgtggcga acggcatcga gcagttgcgc ggtcccgacc tcgggctgct
     1561 gctcatcacg cactaccagc ggatcctcaa ctacgtcacg ccggaccacg tgcacgtcct
     1621 gatggccggg cggatcgtgc ggagcggcgg cgccgagctc gcccacgagc tcgaggagaa
     1681 gggctacgag ggcatgcgta cggaggtcgg cgtggtgggc ggggc
//
LOCUS       DASZOB010000179         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_124761, whole genome shotgun sequence.
ACCESSION   DASZOB010000179 DASZOB010000000
VERSION     DASZOB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124761"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VG709_02950"
     CDS             <1..793
                     /locus_tag="VG709_02950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWC32064.1"
                     /translation="GRTGRTVLRGHRAIRWRARYAGGAKLYPGQYGAALIATDEAGND
                     RRSAEAPWRVHRKVRAHVYTRLPSAGRRVALTFDDCFREDSWRSILRTLRQRRTDATF
                     FCTGAAAARAPSLLRRTVRDGHVFGSHTWDHARLTTVGRSGTANRIARVGALSWSVAR
                     HTTAPYFRPPFGLWDSEVLAGARAASHPRVIMWDVDPRDWTFPGMRTIVRRSVGPARG
                     GSIILLHTIPQTAHALRGIITGLRRKGLRPVTVAELFRAAGYRSG"
     gene            complement(790..1233)
                     /locus_tag="VG709_02955"
     CDS             complement(790..1233)
                     /locus_tag="VG709_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWC32065.1"
                     /translation="MSCVHDEDLAKLRKVALFSVLSEEELDRFARAGKTVEHSSGETL
                     VEEGAVGFRFYLILSGAAVVERGAEEVARIGPGDFVGEISLLGGGHATATVRCTEPTQ
                     CFTLQRERFWEVLEAEPRIALRILEVVARRLEQELHEKQRESLSD"
     gene            1266..1511
                     /locus_tag="VG709_02960"
     CDS             1266..1511
                     /locus_tag="VG709_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32066.1"
                     /translation="MSERPGCFVCGRPTYDPDKRERPWARGVIGGRLVLVCPTCQSDR
                     PEWAQSLDRCPECGSTRLSLMLGELICRACGHVGAPA"
     gene            1508..1939
                     /locus_tag="VG709_02965"
     CDS             1508..1939
                     /locus_tag="VG709_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32067.1"
                     /translation="MSRGRFVIDSRGGRFAAYGALGWCLEITFTSLHDYVRYRDPSLP
                     ARSSLWMFPIYGLLAYLYEPLHDRLREAPWPVRGAAYGAGIMAIEYASGRALRALVGK
                     APWDYTYAKRHIDGLVRPDYFPYWAAVGLAMERVHDRLTAR"
     gene            1950..>2290
                     /locus_tag="VG709_02970"
     CDS             1950..>2290
                     /locus_tag="VG709_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32068.1"
                     /translation="MEPRTRRALVAALASLSGIAAFLLIRPFAGPLAPYGPRPPGTTY
                     AYGFVVLGAFVPYAVAARASRRGVSLRLAVGGTALLAALLLPAAATQSQDVYAYLFYG
                     KIWSLHGANPYV"
BASE COUNT          300 a          831 c          812 g          347 t
ORIGIN      
        1 cggccgaacc gggcgcacgg tgctgagggg gcatcgtgcg atccggtggc gggcgcggta
       61 cgcgggcggc gcgaagctct acccggggca gtacggggcg gcgctgatcg ccacagacga
      121 ggcggggaac gaccggcgtt cggcggaggc tccgtggcgc gtgcaccgaa aggtccgcgc
      181 acacgtgtac acgcgcctac cgagcgccgg caggcgcgtc gcgctgacat tcgacgactg
      241 cttccgggag gattcgtggc gctcgatcct gcggacgctc cgacagcggc ggaccgacgc
      301 cacgttcttc tgcaccggcg ccgccgccgc gcgtgcgccg tcgctcctcc gaaggaccgt
      361 ccgcgacggg cacgtgttcg gatcgcacac atgggaccac gcgcggctca cgaccgtcgg
      421 gaggtccgga acggcgaacc ggatcgcgcg cgtcggggcg ctctcgtgga gcgtcgcgcg
      481 acatacgacc gctccgtact tccgtcctcc gttcgggctc tgggactccg aggtgctcgc
      541 cggcgcccgt gccgcgtcgc acccacgcgt gatcatgtgg gacgtcgacc ctcgcgactg
      601 gaccttcccc gggatgcgga cgatcgtgag gaggagcgtg gggcccgcgc gcggggggag
      661 catcatcctg ctccacacga tcccccagac ggcgcacgcg ctgcggggca tcatcacggg
      721 gctgcggcgc aagggtctcc gcccggtgac ggtcgcggag ctcttccggg cggccggtta
      781 ccgctcgggc tagtcggaga ggctctcccg ctgcttctcg tgaagctcct gctccagccg
      841 ccgggcgacg acctcgagga tccggagcgc gatccgcggc tcggcctcga gcacctccca
      901 gaaccgctcg cgctggagcg tgaagcactg cgtcggctcg gtacacctga ccgtggcggt
      961 cgcgtgcccg cccccgagga gcgagatctc gccgacgaag tcgcccggcc cgatccgcgc
     1021 gacctcctcg gccccgcgct ccacgaccgc ggcgcccgag aggatcaggt agaacctgaa
     1081 cccgacggcg ccctcctcga ccagcgtctc acccgacgag tgctccaccg tcttgccggc
     1141 tcgcgcgaaa cgatccagct cctcctcgga gagcaccgag aacagcgcaa ccttgcggag
     1201 cttcgcgagg tcctcgtcgt gcacgcacga catgttctca cgagccgtct cctatgcttc
     1261 cgcgagtgag cgagcgaccc gggtgcttcg tatgcgggcg gccgacctac gacccggaca
     1321 agcgagagcg cccgtgggcg cgcggcgtga tcggcgggcg gctggtgctc gtgtgcccga
     1381 cgtgccagtc ggaccgaccg gagtgggcgc agtcgctcga ccgctgcccg gagtgcggca
     1441 gcacacggtt gtcgctgatg cttggcgagc tcatctgtcg cgcctgcggc cacgtcgggg
     1501 caccggcgtg agccgcggcc ggttcgtcat cgatagccgg ggcggccggt tcgccgcgta
     1561 cggcgcgctg ggttggtgcc tcgagatcac cttcacgtcg cttcacgact acgtccggta
     1621 ccgagacccg agcctgccgg cgcgctcgag cctgtggatg tttccgatct acggcctgct
     1681 cgcgtacctc tacgagccgt tgcacgaccg tctccgcgag gccccgtggc cggtccgtgg
     1741 agcggcctac ggcgccggca tcatggcgat cgagtacgcg agcggacggg cgctgcgcgc
     1801 gctcgtcggc aaggcgccgt gggactacac gtacgcgaag cgccacatcg acggcctggt
     1861 gcgcccggat tacttcccgt actgggcggc ggtcgggctc gcgatggaac gcgtccacga
     1921 tcggttgacc gcgcgctgag gggacgtcga tggagccgcg gacgcgacgg gcgctcgtcg
     1981 cggcgctcgc ctcgctctcc ggcatcgcgg cgttcctcct gatacggccg ttcgcgggac
     2041 cgctcgctcc gtacggcccg agaccgcccg ggacgacata cgcgtatggc ttcgtggtgc
     2101 tcggcgcgtt cgttccctac gccgtcgccg cgcgcgcgtc gcgccggggc gtctcgctgc
     2161 gcctcgccgt cggcggtaca gcgctgctcg cggcgctcct cctgccggct gcagcgacgc
     2221 aatcccagga cgtctacgcg tatctgttct acgggaagat ctggtccctc cacggcgcga
     2281 acccctacgt
//
LOCUS       DASZOB010000180         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_24098, whole genome shotgun sequence.
ACCESSION   DASZOB010000180 DASZOB010000000
VERSION     DASZOB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24098"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VG709_02975"
     CDS             <1..747
                     /locus_tag="VG709_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWC32069.1"
                     /translation="DFVIIGAQRAGTTSLFRSLAAHPDGGVPSEKELHFFTFNFGRGA
                     DWYREQFPVDAPLTGEATPYYVFHPRAHERMAALVPDAKLIVVLRNPADRAYSHWAFV
                     RAEGHEDLPFEAALEAEERRLAGEVERMLADDGYYSYAHQYHSYLARGRYAEQLERWF
                     AAFARNRFLVLRSEDLYREPARVYDEVMGFLGLRPWTPPAFERYNPSDAIEMDEATRR
                     SLEARFAPLNARLYELLGRDMRWGSSGRTP"
     gene            744..1454
                     /locus_tag="VG709_02980"
     CDS             744..1454
                     /locus_tag="VG709_02980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC32070.1"
                     /translation="MRRWRARRGRSNVEREVEDLAARDPDWHRKAVGGLWDEVGRLQF
                     RFLVEEGLRPDHDLVDIGCGSLRGGVHFVEYLERGRYAGVDVDERLLVAGREELRRRG
                     LEGKEPLLVPTGDFDLPSLGRRFDFALAQSLFTHLPINDIIRCLVRVDDVLEPGGRFY
                     ATFFENPDGKRNVDPISHPTVDLPELLSYFDRDPFHYDVDTFRWMCESTRLEVAYLGE
                     WGHPRDQRMLVFTKRADA"
BASE COUNT          223 a          550 c          539 g          255 t
ORIGIN      
        1 gacttcgtca tcatcggcgc ccagcgcgcg ggcacgacct cgctcttccg gtccctcgcg
       61 gcgcatccag acggcggcgt cccgtcggag aaggagctgc acttcttcac gttcaacttc
      121 ggacgcggcg ccgattggta ccgcgagcag ttccccgtcg acgcgccgct caccggcgag
      181 gcgacgccct actacgtctt ccacccgagg gcgcacgagc gcatggcggc gctggttccc
      241 gatgcgaagc tgatcgtcgt gctgcgcaac ccggcagatc gcgcgtactc gcactgggct
      301 ttcgtccgcg ccgagggcca cgaggacctg ccgttcgagg ccgcgctcga ggcggaggaa
      361 cgccggctcg ccggcgaggt cgagcggatg ctggccgacg acggctacta cagctacgcg
      421 caccagtacc actcgtacct cgcgcggggg cgctacgcgg aacagctcga gcgttggttc
      481 gcggcgttcg cccggaaccg attcctcgtg cttcgcagcg aggatctgta ccgagaaccc
      541 gctcgcgtgt acgacgaggt catgggcttc ctcggcctgc gtccgtggac gccaccggcg
      601 ttcgagcggt acaacccctc cgacgccatc gagatggatg aggcgacgcg ccggtctctc
      661 gaggcgcggt tcgcgccgct gaacgcgcgg ctgtacgagc tcctcgggag ggacatgcgt
      721 tggggttctt cgggacggac gccgtgagac ggtggcgtgc ccgtcgcggc cgctcgaacg
      781 tcgagcgcga ggtcgaagac ctcgcggctc gcgaccccga ctggcaccgc aaggccgtcg
      841 ggggtctttg ggacgaggtc ggccgcctcc agttccggtt cctcgtcgag gaggggctcc
      901 gccccgatca cgacctcgtc gacatcgggt gtgggagcct ccgcggcggc gtgcacttcg
      961 tcgagtacct ggaacgcggc cggtatgccg gcgtcgacgt cgacgagcgg ctgctggtgg
     1021 cgggacgcga ggagctgcga cgtcgcggcc tcgaaggcaa ggagccgctc ctcgtcccca
     1081 cgggcgactt cgaccttccg tcgctcggcc ggcggttcga cttcgcgctc gcgcagtcgt
     1141 tgttcacgca cctgccgatc aacgacatca tccggtgcct cgtgcgcgtc gatgacgtcc
     1201 tcgagccggg tgggcggttc tacgcgacgt tcttcgagaa ccccgacggg aagcgcaacg
     1261 tcgacccgat ctcgcacccg accgtcgatc tgcccgagct cctctcgtac ttcgaccgcg
     1321 acccgttcca ctacgacgtc gacaccttcc ggtggatgtg cgagagcacc cggctcgagg
     1381 tcgcgtacct cggcgaatgg ggacacccgc gcgaccagcg gatgctggtc ttcacgaaac
     1441 gcgcggatgc gtagccgccc ggtcgtcgtc tgcgtcctcg ggatgcaccg gagcgggacg
     1501 tcgctcgcgg ccgggatcct gggccagctc ggcgtctacc tcggcaccga gcggaacatg
     1561 atggcgc
//
LOCUS       DASZOB010000181         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_24122, whole genome shotgun sequence.
ACCESSION   DASZOB010000181 DASZOB010000000
VERSION     DASZOB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24122"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VG709_02985"
     CDS             complement(<1..1357)
                     /locus_tag="VG709_02985"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HWC32071.1"
                     /translation="MLSALLAAPIATASDRHPLPGLPAAAGDALTRALAHGELSEAQY
                     ALERARSLFRLGAVRARFGAVQRPGPRDATMILRDLALVLPQFRASERRAAEGLLARP
                     EDGASDPYGDGYTVASHRQCGTNVCIHWVESTADAPPGADGDGATVPAWVNTTQAVVE
                     DVWQQEVVDFGYRAPLSDSDTSGQGGTADDAKFDVYLANLGDDRLFGYCATDDPDAFT
                     EGPYYDVSAYCVLDNDYAEFGLDPLDILGVTTAHEFFHAVQYAYDFTEDVWFLESLST
                     WIEDELAANATIGIDYDAVNDNVNYLAASPLTYPTVPLDRGPRIDPNDPFRYGGWIFW
                     RFLADRLFADRTAVRDVLERADASAVAIDVGRDEYSTQALARYLASRSRPFRLAFANF
                     ALVNRLRLYADEGSEAAYPIPPLSAKYSIGRTAPSTRWRAPRLRHLASRDYAFKPSKS
                     AR"
     gene            1459..>1890
                     /locus_tag="VG709_02990"
     CDS             1459..>1890
                     /locus_tag="VG709_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWC32072.1"
                     /translation="MTVAEQVAHEKVVSVLDHGFLALDGALASDLAVVNGARVSFNAE
                     SQELADRDAGLIRFLMRERHGSPFEHGYFRFLVKAPLFVVREHHRHRAGHSYNEWSGR
                     YSKLEAEFYVPEYVRSQIGKPGAYSFEAVDAETREAARREIE"
BASE COUNT          302 a          626 c          668 g          294 t
ORIGIN      
        1 tcctcgcact tttcgacggc ttgaatgcgt agtcgcggct cgcgaggtgc cgcaggcggg
       61 gcgctctcca acgcgtgctc ggggccgtcc tgccgatgga gtacttcgcc gagaggggtg
      121 ggatcgggta ggcggcctcg ctcccctcgt ccgcatacaa acgcaggcgg ttcaccaacg
      181 cgaagttcgc gaaggcaagc cgaaacggcc tggaacggga cgcgaggtag cgcgcgagcg
      241 cctgcgttga gtactcgtcg cggccgacgt cgatcgcgac ggcgctcgcg tcggcacgtt
      301 cgaggacgtc gcggactgcg gtgcggtcgg cgaacaaccg atcggccaag aagcgccaga
      361 agatccagcc gccgtacctg aacgggtcgt tggggtcgat ccgcgggccg cggtcaagcg
      421 gcacggtcgg gtacgtcaga gggctggctg cgagatagtt gacgttgtcg ttgaccgcgt
      481 catagtcgat gccgatcgtc gcgttcgccg ccaactcgtc ttcgatccag gtggacaggc
      541 tctcgaggaa ccacacgtct tccgtgaagt cgtatgcgta ctggacggca tgaaagaact
      601 cgtgggcggt agtgaccccg aggatgtcga gcgggtcgag cccgaactcg gcgtagtcgt
      661 tgtcgagcac gcagtaggcg gatacgtcgt agtagggacc ctcggtgaag gcgtcggggt
      721 cgtccgtcgc gcagtagccg aagaggcggt cgtcgccgag gttcgccagg tacacgtcga
      781 acttcgcgtc gtccgcggta ccgccttggc cggacgtgtc cgagtcggac agcggggccc
      841 ggtacccgaa gtcgaccacc tcctgctgcc acacgtcttc gacgaccgcc tgcgtcgtgt
      901 tcacccaggc gggcacggtc gcaccgtcgc cgtcggcgcc gggcggcgcg tcggccgtgc
      961 tctcgaccca gtggatgcac acgttcgtcc cgcactgccg atggctcgcc acggtgtagc
     1021 cgtcgccgta cgggtcgctc gcgccgtcct cgggccgagc caggaggccc tctgccgccc
     1081 ggcgctcgct cgcgcggaac tgcggaagga cgagcgcgag gtcgcggagg atcatcgtcg
     1141 cgtcgcgtgg ccccggccgc tgaacggcgc cgaagcgagc gcgaacggcg ccgaggcgga
     1201 agagcgagcg cgctcgctcg agcgcgtact gcgcctcgct caactcaccg tgcgcgagcg
     1261 cgcgagtaag cgcgtctccc gccgccgcag gcagtccggg gagcgggtgc cgatcggaag
     1321 ccgtcgcgat cggcgcggca aggagcgcgg agagcaccga aaggcagaca gccgtgcgcc
     1381 gccaggcgat cgaactccag gccctctcca cggatccagc ggtcggtgct ctcatccctg
     1441 ctcgttacac tcgttcgggt gactgtcgca gagcaggtcg cgcacgagaa ggtcgtttcg
     1501 gtgctcgacc acgggttcct cgctctcgac ggggcactcg cctcggacct cgccgtcgtg
     1561 aacggcgcgc gcgtctcctt caacgccgag tcacaggagc tggccgaccg cgacgcgggg
     1621 ctcatccgct tcctcatgcg cgaaaggcat ggatcaccgt tcgagcatgg ctacttccgg
     1681 ttcctcgtca aggcgccgct cttcgtcgtc cgcgagcacc accgtcacag agccgggcac
     1741 agttacaacg agtggagcgg acgctattcg aagctcgaag ccgagttcta cgtccccgag
     1801 tacgtgcgca gccagatcgg caagccgggc gcgtacagct tcgaggcggt cgacgccgag
     1861 acgcgcgagg cagcgcggcg cgagatcgag
//
LOCUS       DASZOB010000182         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84625, whole genome shotgun sequence.
ACCESSION   DASZOB010000182 DASZOB010000000
VERSION     DASZOB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84625"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VG709_02995"
     CDS             <1..843
                     /locus_tag="VG709_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWC32073.1"
                     /translation="PMPYRYRCPYCSEKSACTLQCADEVDNIIRNEDPQTVAAVIMEP
                     VQNSAGSITPPDGYFRRIREICDEHGVLLVADEVICGFGRVGEWFGTTRYGIEPDMMT
                     MAKGLTSAYAPLGALVASDKVVEPFFKEPKGMFVHGITFGGHPLSCAIALANIDVFER
                     EDLIGHVQRHMGDFKAAFERVGAEHPMVGDVRGDGFFYSLELVKDKATKATFPPAERD
                     ELIKKFFVPRARELGVYMRVDDRAETAAQFAPPLVAGRSELDELLGVLRQVLDEAADR
                     WLSG"
     gene            901..>2362
                     /locus_tag="VG709_03000"
     CDS             901..>2362
                     /locus_tag="VG709_03000"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC32074.1"
                     /translation="MATTTRTLQNFIGGEWVEATGASAREIVSPVTGEKLADAPNASA
                     EDVDDAARAARAAQPGWGALSPWERAKVCHDIADLIEHRKEEFARELALEQGKPYVAE
                     AIPDIDETAENFRIAAEDVKRLESAILPSQDVNKRILTFRKPNGVYGCVTPWNFPTLI
                     PVELIAPGIAAGNTIVIKPSEWTPIAMANFMQIMADAGLPEGVVNVIYGAGDVGELLV
                     TNANVDAIGFVGSHATAEKIVRAAGLRRSLIEASGNGPVIVCADADLALAAKGAVFGG
                     VYCAGQVCCATERVLVARRVHEEFLAAVVAEAETWTLGDPFDDSTLVGPMNNEPTAQK
                     MDRHVAAAVEKGAQVVFGGSRDNGRPTDLYYQPTVVDRVGVDTLINRDETFGPIVPVI
                     PVGSDDEALAVANDSHLGLQAAVYTRSLGRAFRFLDHLRVGNVVVNDSSDYWEAHEPF
                     GGAAGTRSGWGRIGGRYTMLDMTDLKTVVLDVGSLAD"
BASE COUNT          384 a          800 c          852 g          326 t
ORIGIN      
        1 ccgatgccgt accgctaccg ctgcccgtac tgctcggaga agagcgcctg cacgctccag
       61 tgcgcggacg aggtcgacaa catcatccgg aacgaggacc cgcagacggt cgcggccgtg
      121 atcatggagc cggtccagaa ctcggccggc tcgatcacgc cgccggacgg ctacttccgg
      181 cgcatccggg agatctgcga cgagcacggc gtcctcctcg tcgcggacga ggtgatctgc
      241 ggcttcggcc gcgtcggcga gtggttcggc acgacgcgct acgggatcga gccggacatg
      301 atgaccatgg cgaagggcct gacgagcgcc tacgcgcccc tcggcgcgct cgtcgcgagc
      361 gacaaggtcg tggagccctt cttcaaggag ccgaagggga tgttcgtgca cgggatcacg
      421 ttcggcgggc atccgctctc gtgcgcgatc gcgctcgcga acatcgacgt cttcgagcgc
      481 gaggacctga tcgggcacgt gcagcggcac atgggcgact tcaaggccgc gttcgagcgc
      541 gtcggcgccg agcacccgat ggtcggcgac gtgcgcggcg acgggttctt ctactcgctc
      601 gagctcgtga aggacaaggc gacgaaggcg acgttccccc cggccgagcg cgacgagctg
      661 atcaagaagt tcttcgtccc gcgcgcgcgc gagctcggcg tctacatgcg cgtcgacgac
      721 cgtgccgaga cggccgcgca gttcgcgccg ccgctcgttg cgggtcgctc cgagttggac
      781 gagttactgg gcgtcctgcg ccaggtgctc gacgaagccg cagatcggtg gctctccgga
      841 tagcggcgcc gccgcgcggg gcggggcccg cgagccccga ccgctgaccg aggagggacg
      901 atggctacga cgacacggac gctgcagaac ttcatcggcg gcgaatgggt ggaggcgacg
      961 ggagcgagcg cgcgggagat cgtctcgccg gtgacgggcg agaagctcgc agacgcgccg
     1021 aatgcgagcg ccgaggacgt cgacgacgcc gcgcgcgcag cacgcgcggc gcagcccgga
     1081 tggggcgcgc tctcaccgtg ggagcgcgcg aaggtgtgcc acgacatcgc cgatctcatc
     1141 gagcaccgga aggaggagtt cgcccgcgag ctcgcgctcg agcaggggaa gccctacgtc
     1201 gccgaggcga tccccgacat cgacgagacc gcagagaact tccgcatcgc ggccgaggac
     1261 gtgaaacgcc tggagagcgc gatcctgccg tcgcaggacg tgaacaagcg catcctgacc
     1321 ttcaggaagc cgaacggcgt ctacggatgc gtgacgccct ggaactttcc gacgctcatc
     1381 ccggtggagc tgatcgcgcc cgggatcgcg gccgggaaca cgatcgtgat caagccgtcc
     1441 gagtggacgc cgatcgcgat ggcgaacttc atgcagatca tggccgacgc ggggctcccc
     1501 gaaggcgtcg tcaacgtgat ctacggcgcg ggggacgtcg gcgagctcct cgtcacgaac
     1561 gcgaacgtgg acgcgatcgg gttcgtcggc tcgcacgcga cggcggagaa gatcgtgcgc
     1621 gccgcgggcc tccggcgctc gctgatcgag gcgagcggca acggcccggt gatcgtgtgc
     1681 gcggacgccg acctcgcgct cgccgcgaag ggagccgtct tcggcggcgt ctactgcgcc
     1741 ggccaggtct gctgtgcgac cgagcgggtg ctggtcgccc ggcgcgttca cgaggagttc
     1801 ctcgccgcgg tcgtcgcgga ggcggagacg tggacgctcg gcgacccgtt cgacgactcg
     1861 acgctcgtcg ggccgatgaa caacgagccg acggcgcaga agatggaccg tcacgtcgcg
     1921 gccgccgtcg agaagggggc gcaggtcgtc ttcggcggct cgcgtgacaa cggccgcccg
     1981 accgatctct actaccagcc gacggtcgtc gaccgcgtgg gcgtcgacac gctcatcaac
     2041 cgcgacgaga cgttcgggcc gatcgtcccc gtcatcccgg tcggctccga cgacgaggca
     2101 ctcgccgtcg cgaacgactc gcacctcggc ctccaggccg ccgtctacac gcgctcgctc
     2161 gggcgagcgt ttcgcttcct cgaccacctg cgcgtcggca acgtcgtcgt gaacgactcg
     2221 agcgattact gggaggccca tgagccgttc ggcggcgccg cggggacgag gagcggctgg
     2281 ggcaggatcg gcgggcgcta cacgatgctc gacatgaccg acctcaagac cgtcgtcctc
     2341 gacgtcggca gcctcgccga cc
//
LOCUS       DASZOB010000183         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_64690, whole genome shotgun sequence.
ACCESSION   DASZOB010000183 DASZOB010000000
VERSION     DASZOB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64690"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VG709_03005"
     CDS             complement(<1..451)
                     /locus_tag="VG709_03005"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWC32075.1"
                     /translation="MLLAVNVGNSETSLGVFREREPAWRWRMRTAPERTADELALLFG
                     GFLGQQGLSFDRHVTGVVISSVVPTATRSLREMVERYFGFPPVVVEPGVKTGIPVRVD
                     NPREVGADRVVNALAALEKYGAPAIVVDVGTATTFDVGPPEGEYVGGA"
     gene            complement(454..1323)
                     /gene="panC"
                     /locus_tag="VG709_03010"
     CDS             complement(454..1323)
                     /gene="panC"
                     /locus_tag="VG709_03010"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWC32076.1"
                     /translation="MEVIETRGAYRDACERARQRGRDVGFVATMGFLHEGHRSLLGRA
                     RDDNAFVAMSIFVNPLQFGPAEDLESYPRDLDRDVSLAEKDGVDVVFAPSADEMYARG
                     RPGVTVDPGPLGERFEGTARPGHFRGVCTVVAKLFALTGPCRAYFGEKDAQQLAVIRA
                     MVRDLEIPVEVVGCPTVREEDGLAMSSRNGYLSEEERRAATCLYEALARARWLVEGGE
                     RDANVLRAEVAKRVGAEPLARIEYAAVVDEETFDEVDVLRRPARALVAATLGNARLID
                     NLPLPVDDEDGGD"
     gene            complement(1314..2177)
                     /locus_tag="VG709_03015"
     CDS             complement(1314..2177)
                     /locus_tag="VG709_03015"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HWC32077.1"
                     /translation="MDVGVVGAGRVGTAVAVLLRRAGHRVLAASGRDRSRERVERFLA
                     GVAFRSSTETASAAEVVIVAVPDDRIETVAREIGHGGGFRRGQAVVHLSGSAALAALE
                     AARESGARTLSVHPLQTAPSVEDALERLPGSAVAVTALDEEGYVLGEALAIDIGGRPF
                     RLADDRKPLYHAAAVFASNYVAAVAGIAERLFREAGLDDPVSLFGPLTEATAANVARL
                     GPAGALTGPAARGDAGTVRANLEALRPLGAEVADAYVALGRAAAGLATAAGRISADDR
                     ARVEEVLRAWR"
     gene            complement(2265..2759)
                     /gene="folK"
                     /locus_tag="VG709_03020"
     CDS             complement(2265..2759)
                     /gene="folK"
                     /locus_tag="VG709_03020"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWC32078.1"
                     /translation="MTRAFLGLGSNEGDRLAALQRAVELLRARGVRILRSSRVYETDP
                     VGGPSQPDYLNAVIEVEVDGSAYDLLDACRTVEEEMGRVRLERWGPRAIDLDVLTFGG
                     EEIDEPDLQVPHPRMHERGFVLVPLLELDADPRLPGGRKIASLKLGAAAITGVRPYAP
                     PLAV"
     gene            complement(2756..3109)
                     /locus_tag="VG709_03025"
     CDS             complement(2756..3109)
                     /locus_tag="VG709_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWC32079.1"
                     /translation="MAQRLKISGIRAQGRHGARPGERDSPQPFVVDLELDVDAHDDDL
                     ATTADYRDVIAAVRGIVEGESYSIIETIVERVVDVIAAYPGVRGVIARVHKPAAADRL
                     GVADVLAEAAAGSLS"
BASE COUNT          444 a         1214 c         1160 g          434 t
ORIGIN      
        1 tggcgccgcc gacgtactct ccctccggcg ggccgacgtc gaacgtcgtc gcggtgccca
       61 cgtcgacgac gatcgccggc gcgccgtact tctcgagcgc tgcgagcgcg ttgacgacgc
      121 ggtccgcccc gacctcgcgc gggttgtcga cgcgcacggg gatgccggtc ttcaccccgg
      181 gctccacgac gacgggcggg aagccgaagt accgctccac catctcgcgg agggagcgcg
      241 tcgccgtcgg gacgacgctc gagatcacga cgccggtgac gtgacggtcg aaggagagtc
      301 cctgctgtcc gaggaacccg ccgaacagca gcgcgagctc gtccgcggtg cgctcgggcg
      361 ccgtccgcat cctccagcgc cacgcgggct cgcgctcgcg gaacaccccg aggctcgtct
      421 cgctgttccc gacgttgacc gccagcagca tcgtcagtcg cctccgtctt catcatccac
      481 ggggagcggg aggttgtcga tgagccgggc gttcccgagc gtcgccgcga cgagcgcgcg
      541 cgccggtctc cgaaggacgt cgacctcgtc gaaggtttcc tcgtccacga ccgccgcata
      601 ctcgatccgc gcgagcggct ccgcgccgac ccgcttggcg acctcggcgc gcaggacgtt
      661 ggcgtctcgc tcgccgccct cgacgagcca gcgcgctcgg gcgagcgcct catagaggca
      721 cgtcgcggcc cggcgctcct cctcggagag gtacccgttc cgtgacgaca tcgcgagccc
      781 gtcctcttcg cgcaccgtcg ggcagcccac gacctccacc gggatctcga ggtcgcggac
      841 catcgcccgg atcaccgcga gctgctgcgc gtccttctcg ccgaagtagg cgcggcacgg
      901 acctgtgagc gcgaacagct tcgcgaccac cgtacagacg ccgcggaagt ggccggggcg
      961 tgcggtgccc tcgaaccgtt cgccgagcgg gccgggatcg acggtgacgc cgggacggcc
     1021 acgcgcgtac atctcgtcgg ccgacggcgc gaacacgacg tcgacgccgt ccttctccgc
     1081 gagcgagacg tcccggtcga ggtcgcgcgg gtacgactcg agatcctcgg ccggcccgaa
     1141 ctggagcggg ttgacgaaga tcgacatcgc gacgaacgcg ttgtcgtcgc gggcgcgtcc
     1201 gaggagcgag cggtgcccct cgtgcaggaa ccccatcgtc gcgacgaagc cgacgtcgcg
     1261 tcctcgctgc cgcgcccgct cgcatgcgtc ccggtaggcc ccgcgcgtct cgatcacctc
     1321 cacgcgcgca gcacctcctc gaccctcgca cggtcgtccg cggagatccg cccggcggcc
     1381 gttgcgagac cggccgccgc gcggccgagc gcgacgtagg cgtcggccac ctcggcgccg
     1441 agcggtcgaa gcgcctcgag gttcgccctg acggtcccag cgtcgccgcg cgcggcaggc
     1501 cccgtgagcg cgccagcggg accgagccgt gcgacgttgg cggcggtcgc ctccgtgagc
     1561 ggaccgaaca gcgacacggg gtcgtcgagg ccggcctcgc ggaagagccg ctcggcgatg
     1621 ccggcgaccg cggcgacgta gttcgaggcg aacacggctg cggcgtggta cagcggcttc
     1681 cggtcgtcgg cgaggcggaa cggtcggccg ccgatatcga tggcgagcgc ctccccgagc
     1741 acgtatccct cctcgtcgag cgccgtcacc gcgacggcgc ttcccggtaa ccgctcgagc
     1801 gcgtcctcga cgctcggtgc ggtctggagc gggtggaccg acagcgtgcg cgctccgctc
     1861 tctcgagcgg cctcgagggc ggcgagcgcg gcggagcccg acaggtgcac gacggcttgt
     1921 ccacggcgga agcccccccc atgtccgatc tcgcgcgcga cggtctcgat gcggtcgtcg
     1981 gggacggcga cgatcacgac ctcggcggcg gatgccgtct cggtcgacga gcggaacgcg
     2041 acgccggcga ggaaccgctc gacgcgctcc cgggagcggt cgcgtccact ggcggcgagg
     2101 acgcggtggc cggcacggcg aagcagcacc gcgacggccg tcccgaccct gccggcccct
     2161 acgacgccga cgtccacggg gtccggcggg tcacgttggg ggtagatcgc tcgatgttcc
     2221 ggtccttcct cggctccgga cgcggcgagt atacggagtg gctctcagac ggcgagcggc
     2281 ggcgcgtacg ggcggacgcc ggtgatcgcg gccgcgccga gcttcagcga cgcgatcttg
     2341 cgcccgccgg gaagccgcgg gtcggcatcg agctcgagca gcggcaccag cacgaaacct
     2401 cgctcgtgca tccgcgggtg gggcacctgg aggtcgggct cgtcgatctc ctctccgccg
     2461 aacgtcagga cgtcgaggtc gatcgctctc ggcccccacc gctccaggcg aacacgcccc
     2521 atctcctcct ccacggtgcg acacgcgtcg agcaggtcgt acgcggagcc gtcgacctcg
     2581 acctcgatca ccgcgttcag gtaatccggc tgcgacggcc cgccgacggg atcggtctcg
     2641 tacacgcggg acgatcgaag gatccggacg ccgcgcgcgc gcaggagctc gaccgctctc
     2701 tggagcgcgg ccagccggtc gccctcgttc gacccgagcc cgaggaacgc tcgcgtcacg
     2761 agagcgaccc cgccgccgcc tcggcgagga cgtcggcgac gccgagccgg tcggcggccg
     2821 cgggcttgtg caccctcgct atcacgccgc gcacgccggg atacgccgcg atcacgtcga
     2881 cgacgcgttc gacgatcgtc tcgatgatcg agtacgactc cccctcgacg atgccgcgga
     2941 cggcggcgat cacgtctcgg tagtccgcgg tcgtggcgag gtcgtcgtcg tgggcatcga
     3001 cgtcgagctc gaggtcgacg acgaacggct ggggggagtc gcgctcaccc ggacgggcgc
     3061 cgtgacgtcc ctgcgcgcgg ataccggaga tcttcaggcg ctgcgccacg tcatccggcc
     3121 cccgttcgtg ccgcggcgcg gcgggcacgc gcctcgggga tcttccggaa gtcccacgac
     3181 gtgatgtcct gcggcacgag ccgcagccac acccgatcgc gccagtgccg gtatggggtc
     3241 ggggcgccgc cg
//
LOCUS       DASZOB010000184         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_165424, whole genome shotgun sequence.
ACCESSION   DASZOB010000184 DASZOB010000000
VERSION     DASZOB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165424"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VG709_03030"
     CDS             complement(<1..688)
                     /locus_tag="VG709_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC32080.1"
                     /translation="MGDVELQSIEALLEERRVIEPPESFHADALVRDDSIYREAADDL
                     EGFWAREGERIDWFEKWDRLLEWDPPWQKWFVGGKLNVAHNCVDRHVEAGLGDRVAYH
                     WEGEPGDQETITYERLRRDVVRTANVLKSLGVRKGTPVGIYMGMIPRLPVAMLACARL
                     GAPHTVVFGGFSAEALSDRLNDMGCEVLITQDEGWRRGQRVPLKRNADAALESAPGVK
                     HVLVARRTGGD"
     gene            770..1744
                     /locus_tag="VG709_03035"
     CDS             770..1744
                     /locus_tag="VG709_03035"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC32081.1"
                     /translation="MRAFVTGATGFIGGYVARALRERGDDVVALVRTPPKAKELEALG
                     CRLIPGSLDAIGSIEDGVRGADAVFHAAAIYKAGIPRKARPEMYEANVTGTERVLDVA
                     IAAGVPRIVHVSTVNVFGNTKRKVVDETYVRDPRDGFLSYYDETKFLSHQIALDRIAQ
                     GARVVIAQPGGVYGRRDPSDLGNAIRQIASGRLKFKAFPDTGFVFLHVEDAADGILRV
                     HDKGGVGDSYVLGGERSTLGAFIDLVARLAGRKPPRVTVPAPLVKAGIPFGRLIGKAM
                     GTGPNLRELIRAADGVTYWASDGRARRELGYAPRDLETGVRQTLAAGA"
     gene            complement(1716..2420)
                     /locus_tag="VG709_03040"
     CDS             complement(1716..2420)
                     /locus_tag="VG709_03040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC32082.1"
                     /translation="MSTEPISRFFDREAEACCRHGDDEPVGVSGVSSVLLEYVERTGI
                     EGRTLLDLGCGTGGFVIEALRRGAASGLGVDVSSVSIEAASRLARFAGLGERAGFRIG
                     DASAVHLERHDVVVLNKSICCYPKAEALVENAVRAASHVCAFSVPVSRGVRGLVARTT
                     VRLENAWRAIRRDPFRAYVHPTDAIEAKLRAARLERVASVRWWTWYVGVFVREPVEVA
                     ASPMAAAARRRQPASA"
     gene            complement(2428..2823)
                     /locus_tag="VG709_03045"
     CDS             complement(2428..2823)
                     /locus_tag="VG709_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWC32083.1"
                     /translation="MAHSNADLARRELQAVNAGDLAALREIYTEDFVFHYPGRSPLAG
                     DYRGFDGLRDFAEKVGDVAGEQMEREAHAIVADDEHAVQLLTVRATRKDGRTHEWNAV
                     VVLHLRDGKFNEAWVHVDDPYAHDEFLSG"
BASE COUNT          435 a         1073 c          998 g          428 t
ORIGIN      
        1 cgtcgccgcc ggtgcggcgc gcgaccagga cgtgcttcac cccgggcgcc gactcgagcg
       61 cggcgtcggc gttgcgcttg agcggcacgc gctgtccgcg ccgccatccc tcgtcctggg
      121 tgatcaggac ctcgcagccc atgtcgttca ggcggtcgga gagcgcctcc gcggagaacc
      181 cgccgaagac gaccgtgtgc ggcgcgccga gccgcgcgca cgccagcatc gcgaccggaa
      241 gtctggggat catgcccatg tagatcccga ccggcgttcc ctttcgcacg ccgagcgact
      301 tcaggacgtt ggcggtgcgg acgacgtcgc ggcggagccg ctcgtacgtg atcgtctcct
      361 ggtcgcccgg ctccccctcc cagtgatagg cgacgcggtc gccgagcccg gcctcgacgt
      421 gacggtcgac gcagttgtgg gcgacgttga gcttgccgcc gacgaaccac ttctgccagg
      481 gcgggtccca ttcgagtagg cgatcccact tctcgaacca gtcgatccgc tcgccctcgc
      541 gggcccagaa cccctcgaga tcgtccgcgg cctcgcggta gatcgagtcg tcgcggacca
      601 gcgcgtccgc gtggaagctc tcgggtggct cgatgacccg ccgctcctcg agcagcgcct
      661 cgatgctctg gagctcaacg tcacccaact gtgctcacct cctccgcgcc gccacgatag
      721 aacggacgcc ggcccgagcg cgacgccggc gcgcactaca ctccgcgccg tgcgagcgtt
      781 cgtcaccggc gcgaccggct tcatcggcgg ctacgtcgcg cgggctctac gcgagcgagg
      841 cgacgacgtc gtcgcgctcg tgcgaacccc cccgaaggcg aaggagctcg aggcgctcgg
      901 ctgccgcctc atcccggggt ccctcgacgc gatcggatcg atcgaggacg gcgtgcgcgg
      961 cgccgacgct gtcttccacg cagccgcgat ctacaaggcg ggcatcccgc ggaaggcgag
     1021 gccggagatg tacgaggcca acgtcaccgg gaccgagcgc gtcctcgacg tcgcgatcgc
     1081 cgccggcgtg ccgcggatcg ttcatgtctc gaccgtcaac gtcttcggga acaccaaacg
     1141 caaggtcgtc gacgagacgt acgtccgcga tccgcgcgat gggttcctct cctactacga
     1201 cgagacgaag ttcctctcgc accagatcgc gctcgaccgg atcgcgcagg gcgcgcgagt
     1261 cgtgatcgcg cagcccggcg gcgtgtacgg gcggcgcgac ccatcggatc tcgggaacgc
     1321 gatccggcag atcgcgtcgg gacgcttgaa gttcaaggcg ttcccggaca ccgggttcgt
     1381 gttcctccac gtggaggacg cggccgacgg catccttcgc gtccacgaca agggaggcgt
     1441 cggggactcg tacgtcctcg gcggcgaacg ctcgacgctc ggcgcgttca tcgatctcgt
     1501 cgcccgcctc gccggtcgga agccgccgcg cgtgacggtg ccggcaccgc tcgtcaaggc
     1561 cggcatcccg ttcgggcggc tgatcggcaa ggcgatgggg acggggccga acctccgaga
     1621 gctcatccgc gcggccgacg gcgtcacgta ctgggcgtcg gacgggcgag cgcgccgcga
     1681 gctcggctac gccccccgcg acctcgagac gggggtcagg cagacgctgg ctgccggcgc
     1741 ctagcagcgg ctgccatcgg ggacgccgcc acctcgaccg gctcgcggac gaacacgccc
     1801 acgtaccagg tccaccaccg aaccgaggca acgcgttcga gccgggccgc ccggagcttc
     1861 gcctcgatgg catccgttgg atgcacgtac gcacggaacg ggtcccgacg gatcgcccgc
     1921 catgcgttct cgagacgcac cgtcgtcctt gcgaccagcc cgcgcacgcc acgcgagacg
     1981 gggacgctga aggcgcagac gtgagaggcc gcgcgcacgg cgttctcgac gagcgcctcc
     2041 gctttcggat aacagcagat cgacttgttc aggacgacga cgtcgtgccg ttcgaggtgc
     2101 acggcggacg cgtcgccgat gcggaagccc gcccgttccc cgagtccggc gaacctcgcc
     2161 aggcgactcg ctgcctcgat actgacggag gagacgtcga cgccgagtcc gctcgctgca
     2221 ccgcgacgca gcgcctcgat cacgaagccg ccggttccgc acccgaggtc cagcagcgtc
     2281 cggccctcga tcccggtccg ctcgacgtac tcgagcagga cgctcgagac gcccgagacg
     2341 ccgaccggct cgtcgtcgcc gtggcggcag cacgcctcgg cctcgcggtc gaagaaccgc
     2401 gagatcggtt cggtgctcac accgccctca gccgctcagg aactcgtcgt gcgcgtacgg
     2461 gtcatcgacg tgaacccagg cctcgttgaa cttcccgtcg cggaggtgga gcacgaccac
     2521 cgcgttccac tcgtgcgtcc gcccgtcctt ccgcgtggcg cggacggtga ggagctgcac
     2581 ggcgtgctcg tcgtcggcca cgatcgcgtg cgcctcacgc tccatctgct cgccggcgac
     2641 gtcgccgacc ttctcggcga agtcgcggag cccgtcgaag cctcgatagt cgcccgcgag
     2701 cgggctccgg cccggatagt ggaagacgaa gtcctccgtg tagatctcgc gcagcgcggc
     2761 gagatcgccc gcgttgacgg cctggagctc gcgcctggcg aggtccgcgt tggaatgtgc
     2821 catgtctcct cctagagcgg gacgtgggag aggatcagga tgcgcggctc ggtcatctcc
     2881 tcgatcgcgt agcggaggcc ctcgcgtccc agcccggact ggttgacgcc tccg
//
LOCUS       DASZOB010000185         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84644, whole genome shotgun sequence.
ACCESSION   DASZOB010000185 DASZOB010000000
VERSION     DASZOB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84644"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VG709_03050"
     CDS             complement(<1..434)
                     /locus_tag="VG709_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HWC32084.1"
                     /translation="MTVATVDYHTAGEPFRIVTGGVGPPQGATVLERRRDAAARLDAV
                     RALLINEPRGHADMYGCFVTPPDDEGADLGVVFFHNDGYSTACGHGTIALVTWALESG
                     VLTVTGPETVVVVDTPSGRLRCIASVDGEKVTAVRFENVPSFV"
     gene            complement(464..1792)
                     /locus_tag="VG709_03055"
     CDS             complement(464..1792)
                     /locus_tag="VG709_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32085.1"
                     /translation="MDVPETRYTRAPDGVQIAYQVTGGGNLDLVLMQGAVTHLELQWE
                     DSRLTRFFERLSAFSRLIRFDRRGMGMSEALQQLPTFEEQLGDFGVVMDAVGSERAAL
                     MGNIDAGILALAFAEAHPERTRALIAFEVTPRFARSEADNFGVDPEMVSRMAQAIQAM
                     DIETMASIVAPARVDEPGYLSWFRRYTRSGWSGIPIGAFLMMAGSWDVTGLLPRIEVP
                     VLVLSRAENVIVPIRNGRALAAALPDARLVEIPGTGAVIWSADVDLGADEIEAFLTGT
                     RPPPRRDRVLATVLFTDIVGSTDTAARMGDRDWRELLGRHNRVVRASLERYDGREIHT
                     AGDGFLATFDSPRRAIECARAATSAVRSIGVEIRAGLHTGEIELAGNDVQGIAVHIGA
                     RVSAKAGAGEVLVSSTVKDLVVGSGIDFEDRGTHTLKGVPGEWRLFAVAG"
     gene            complement(1794..2885)
                     /locus_tag="VG709_03060"
     CDS             complement(1794..2885)
                     /locus_tag="VG709_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32086.1"
                     /translation="MDEIRLVMDEAGIERAALFGSSEGSLLSAVFAAAHPKRVSSLVL
                     FGGWARFFPDPPDYPFGMMDVESTELLVEALRDTWGENGEFILSLWAPSAAGDPFAVE
                     EFSRFLRLAASPAAGAALSLRAADGDIRDVLPSIHVPTLVLHRSGDRVAEIGHGRYLA
                     EHIPGARFVELPGNEHLWWYGDHDAVLEHIEEFLTGRRPMVDPDRVLATVMFTDIVDS
                     TQQASALGDQEWREVLGRHDELIRRELARHRGREVKTTGDGFLATFDGPARAVRCAES
                     IRDGVRALGLDVRAGLHTGEVELVGEDIQRIAADIGGRVAAIASPGEVLASSTVKDLV
                     AGSGIEFEDRGAHALKGVPGEWRLFSVKA"
     gene            complement(3142..>4043)
                     /gene="tyrS"
                     /locus_tag="VG709_03065"
     CDS             complement(3142..>4043)
                     /gene="tyrS"
                     /locus_tag="VG709_03065"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWC32087.1"
                     /translation="LEQVRTVLDLERAEIRRNSEWLAALTFSDVATLAATQTVARLLE
                     REDFRDRYEAERPISLVEFLYPLMQGYDSVAVRADVEMGGTDQRFNLLVGRDIQRAHG
                     QEPQVAFTMPLLVGADGERKMSKSLGNHIGLTELPEEQFGKAMSIPDDVMPTWFRLCT
                     DLDPEELERIERGLAEGPMQPAETKRRLAREIVRLYHGDEAARAAEEQFDRVFRQHEL
                     PDDVPERALPDDALDGGAVYLPKLLVAVGLAASNSDARRLIEQGGVRIDGEAVNDPNA
                     DLRADDVRGRVIQVGRRRFVRVT"
BASE COUNT          643 a         1463 c         1339 g          598 t
ORIGIN      
        1 acgaaggagg gcacgttctc gaagcgcacg gcggtcacct tctcgccatc gacgctcgcg
       61 atacagcgga ggcgcccgga gggcgtatcg accaccacga cggtctccgg cccggtcacc
      121 gtcaggacgc cgctctccag cgcccacgtc acgagcgcga tcgtcccgtg accgcaggcc
      181 gtcgagtagc cgtcgttgtg gaagaacacg acgccgaggt cggcaccctc gtcgtcgggc
      241 ggcgtcacga agcacccgta catgtccgcg tgcccgcgcg gctcgttgat gagcagcgcg
      301 cgaacggcgt cgaggcgcgc cgcggcgtct cgccggcgct ccagcaccgt agcgccctgc
      361 ggtggcccca cccctccggt gacgatgcgg aacggttcac cggcagtgtg gtagtcgacg
      421 gtcgcgaccg tcatgggcgt ctgcatcgca gggtgtcggt ccatcaaccg gcaacggcga
      481 acaaccgcca ctcccccggc acgcccttca gcgtgtgcgt accgcgatct tcgaagtcga
      541 tcccggaccc gacgacgagg tccttgacgg tcgacgagac aagcacctcg cccgctcccg
      601 ctttcgccga cacgcgcgca ccgatgtgaa cggcgatgcc ctgtacgtcg ttcccggcga
      661 gttcgatctc gcccgtgtgc agtcccgcgc ggatctcgac accgatcgac cggacggccg
      721 aggtcgcggc gcgtgcgcac tcgatcgcgc gccggggcga gtcgaacgtc gccaggaacc
      781 cgtcgccggc ggtgtggatc tcgcgtccgt cgtaccgctc cagcgacgca cggacgaccc
      841 ggttgtggcg accgagcagc tcgcgccaat cacggtcacc catgcgcgcc gccgtgtccg
      901 tcgagccgac gatgtcggtg aacaggaccg tggccaggac ccggtcccgc cgcggcggtg
      961 gccgcgttcc ggtgaggaac gcctcgatct cgtcggcccc caggtcgacg tccgcggacc
     1021 agatcaccgc gccggtgccc gggatttcca caagccgagc gtccggcagg gcggcagcaa
     1081 gcgcgcgacc gttccggatc ggaacgatga cgttctcggc cctgctgagc acgagcactg
     1141 gcacctcgat ccgcggcagg aggccggtca cgtcccagga cccggccatc attaagaagg
     1201 cgccgatcgg gatcccgctc cagccggacc gggtataccg gcggaaccag gagaggtaac
     1261 ccggctcgtc cacccgcgcc ggggccacga tggacgccat cgtctcgatg tccatcgcct
     1321 ggatcgcctg agccatgcga ctcaccatct cggggtccac gccaaagttg tccgcctcgg
     1381 atcgcgcgaa ccgtggggtt acctcgaaag cgatgagagc ccgggttcgc tccgggtgag
     1441 cctctgcgaa ggcgagggca agaatgcccg cgtcgatgtt gcccatcagt gccgcccgct
     1501 ccgatcccac cgcgtccatg acgaccccga agtccccgag ctgctcctcg aacgtcggga
     1561 gctgctggag ggcctcggac atccccatcc ctcggcggtc gaaccggatc aggcgcgaga
     1621 acgccgacag ccgctcgaag aaccgggtca gccgtgagtc ctcccactgc agctcgagat
     1681 gggtgaccgc gccctgcatc agcaccaggt ccaggttgcc gccgcccgtc acctggtacg
     1741 cgatctggac accgtccgga gctcgcgtgt acctggtctc ggggacgtcc acgtcaggcc
     1801 ttcaccgaga agagccgcca ctcccccggc acgcccttca gcgcgtgtgc gccccggtcc
     1861 tcgaactcga tccccgagcc ggcgacgagg tccttcaccg tagaggaagc gagcacctct
     1921 cctgggctcg cgatcgccgc aacgcggccg ccgatgtcag cggcgatccg ctggatgtcc
     1981 tctccgacga gctcgacctc gccggtgtga aggcccgcac ggacgtcgag cccgagcgcg
     2041 cggacgccgt cgcggatgct ctcagcacac cggacggctc gagccgggcc gtcgaacgtg
     2101 gcgaggaacc cgtcgccggt cgtctttacc tcgcgcccgc ggtgacgcgc cagctcccgg
     2161 cggatcagct cgtcgtggcg gcccaggacc tccctccact cctgatcccc cagtgcactc
     2221 gcttgctgcg tcgagtcgac gatgtccgtg aacatcacgg tggcaagaac ccggtcggga
     2281 tcgaccatcg gtcgcctgcc cgtcaggaac tcctcgatgt gctccaggac cgcgtcgtga
     2341 tccccgtacc accacaggtg ctcgttacca ggaagctcga cgaagcgcgc tccgggaatg
     2401 tgctccgcga ggtagcgccc gtgcccgatc tccgccacgc ggtcgcccga gcggtgcagc
     2461 accaaggtgg ggacgtggat ggacgggagc acgtcccgga tgtcgccgtc ggccgcgcgc
     2521 agcgacaacg ccgcgccggc agcggggctc gcggcgagtc gcaagaatcg gctgaactct
     2581 tcgacggcga agggatctcc cgccgcgctc ggggcccata gagacaggat gaactccccg
     2641 ttctcgcccc aggtgtcgcg cagcgcctcg acgaggagct cggtcgactc gacgtccatc
     2701 atcccgaagg gatagtcggg cgggtcgggg aagaaccgcg cccacccgcc gaagagcacg
     2761 agtgaggaga cccgcttcgg atgcgcggca gcgaagacgg ccgagaggag acttccctcg
     2821 gacgatccga acaaggcggc gcgctcgatc ccggcttcgt ccatgacgag ccggatctcg
     2881 tccatccgct cgtcgagcgt cgggacctga tcgacgggac tcggtccgac agtcccgtcc
     2941 ctcgcttgtc gaacacgatg accctcgcga aacgcgtcag cttggagaga gccggcccgt
     3001 atcgggagtc atcccagatc gcctccacgt ggctgaccca gcctggaacg tagaccacat
     3061 ccagcggtgc ctctccgacg acctggtacg cgatgtgaag ggctccgtcg cgtacgtatc
     3121 gcgtggctgg tcgcacgcgg ctcatgtcac gcggacgaag cgccggcggc cgacctggat
     3181 cacgcggccg cggacgtcgt cggctcggag gtcggcattc gggtcgttca cggcctcgcc
     3241 gtcgatacgg acgccgccct gctcgatcaa gcgccgggca tcggagttcg acgcggccag
     3301 gccgacggcg acgaggagct tcgggaggta gacggcgccg ccgtccagcg cgtcgtccgg
     3361 gagcgcgcgc tccggaacgt cgtcggggag ctcgtgctgg cggaagacgc gatcgaactg
     3421 ctcctccgcc gcgcgagcgg cctcatcgcc gtggtacagg cgaacgatct cgcgcgcgag
     3481 ccggcgcttc gtttccgccg gttgcatcgg cccctccgcg aggccgcgct cgatccgctc
     3541 cagctcctcg ggatcgagat ccgtgcacag acggaaccac gtcggcatga cgtcgtcggg
     3601 gatgctcatc gccttcccga actgctcctc cggaagctcg gtgagcccga tgtgattccc
     3661 gagcgacttc gacatcttgc gctcgccgtc ggccccgacc agcagcggca tcgtgaacgc
     3721 gacctgcggc tcctgtccgt gcgcccgctg gatgtcgcgc ccgacgagaa ggttgaagcg
     3781 ctggtcggta ccccccatct cgacgtccgc ccgcaccgcg acggagtcgt acccctgcat
     3841 gagcgggtaa aggaactcga cgaggctgat cgggcgctcg gcctcgtagc ggtcgcggaa
     3901 gtcctcgcgc tcgagcaacc gtgccacggt ctgcgtcgcg gcgagcgtcg cgacgtcgga
     3961 gaacgtgagc gccgcgagcc attcgctgtt gcgacggatc tcggctcgct cgagatccag
     4021 caccgtccgg acctgttcga ggt
//
LOCUS       DASZOB010000186         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_105221, whole genome shotgun sequence.
ACCESSION   DASZOB010000186 DASZOB010000000
VERSION     DASZOB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105221"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VG709_03070"
     CDS             <1..817
                     /locus_tag="VG709_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWC32088.1"
                     /translation="RYLVPAIRGEKIACLGISEPDAGSDVANIQTTARRDGDGWVING
                     RKTFITNGRRADFSLLVTRTDKPAGYEGFSLFLVDTDLPGFSISRTLDKLGMHSSDTA
                     ELSFDDVRVPASALLGEEGKGFHQIMWELQGERLIGAAGSIAGAWLAWERTLEYAKER
                     TAFGRPIGSFQVQRHRFAEIATELEAARQLLYDVALAWERGEYPVKQISMVKLYSGRV
                     VNRAMNELLQVHGGYGYAAESWIQRAWRDARLLRIGGGTDEVMREIVAKTMEL"
     gene            819..1991
                     /locus_tag="VG709_03075"
     CDS             819..1991
                     /locus_tag="VG709_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC32089.1"
                     /translation="MAETRFAAIPEHGLFTEDHEALRAGVREFVERALRPHAEEWERS
                     GDFPVREVFRDAGRVGIFGAKYEDAYGGTGPDLVADAVITEELVRCASGGVAAALGAH
                     KDLGSYYLYRFGTEEQKRRWLVPAIAGAKISALAVTEPDAGSDVAGIRTRAVRDGDAW
                     ALAGSKTFITNGPVADVLVVAAKTDPDAAHGGISLFVVEAGTPGLVARRIETVGWRTS
                     HTGELVFDRCRVPAENVLGEEGRGFVQIMQSFQWERIVMALSAVAAADLTLEMAIAYA
                     RERHAFGRPVARFQVWRHRFADLATEIEAARSLTYQALRKIVAGEDAVREVSMAKWLG
                     TELDWRVADEAVQVHGGYGYMMEFPAQRAWRDARLGAIGGGTTEIMKEIIGRSYGL"
     gene            complement(1966..2196)
                     /locus_tag="VG709_03080"
     CDS             complement(1966..2196)
                     /locus_tag="VG709_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32090.1"
                     /translation="MTDEFSRATGAARALSVPVWLARPFASYAATFMGGTRLRVSNAK
                     VKRELRWAPGFPTYREGLADVGATLTGRTSGR"
     gene            complement(2193..>2468)
                     /locus_tag="VG709_03085"
     CDS             complement(2193..>2468)
                     /locus_tag="VG709_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC32091.1"
                     /translation="EIHTKERSVLSGGMDGVVLRYGLFYGPGVGSTELMAKLLRRRMM
                     ALPGGGRGVASWIHIEDAAEATVSALERGRAGGSTTSSTTSRSRSER"
BASE COUNT          372 a          839 c          897 g          360 t
ORIGIN      
        1 gcgctatctc gtgcccgcga tccgcggcga gaagatcgcg tgtctcggga tctccgagcc
       61 cgacgccggc tcggacgtcg cgaacatcca gacgacggcg cggcgcgacg gcgacgggtg
      121 ggtgatcaac ggccgcaaga cattcatcac gaacggccgg cgagccgact tctccctcct
      181 cgtgacccgc acggataaac ctgcggggta cgagggcttc agccttttcc tggtcgacac
      241 ggatctcccc gggttctcga tctcacggac gctcgacaag ctcgggatgc acagctcgga
      301 cacggcggag ctctcgttcg acgacgtccg cgtcccggcg tcggcgctgc tcggcgagga
      361 ggggaagggg ttccaccaga tcatgtggga gctccagggc gagcggctga tcggcgcggc
      421 cggctcgatc gccggggcgt ggctcgcatg ggaacgtacg ctcgagtacg cgaaagagcg
      481 gactgcgttc gggcgtccga tcgggtcgtt ccaggttcag cggcaccgct tcgccgagat
      541 cgcgacggag ctcgaggccg cgcggcagct cctctacgac gtcgcgctgg cgtgggagcg
      601 cggcgagtat cccgtcaaac agatctccat ggtcaagctc tattccggcc gcgtcgtgaa
      661 ccgcgcgatg aacgagctgc ttcaggtgca cggcggctac ggctatgccg ccgagtcgtg
      721 gatccagcgc gcgtggcggg acgcgaggct gctacgcatc ggcggtggca ccgacgaggt
      781 catgcgcgag atcgtggcca agacgatgga actctgacgt ggcggagacg cgcttcgcgg
      841 cgatccccga gcacggtctc ttcaccgagg atcacgaggc gctgcgcgcg ggggtccggg
      901 agttcgtcga gcgcgcgctg cggccacacg cggaggagtg ggagcgctcg ggcgacttcc
      961 cggtgcgcga ggtgttccgc gacgccgggc gcgtcggcat cttcggcgcg aagtacgagg
     1021 atgcctacgg cgggacgggg cccgacctcg tcgccgacgc ggtcatcaca gaggagctcg
     1081 tgcggtgcgc gtccggtggc gttgcggcgg cgctcggcgc gcacaaggac ctcggctcgt
     1141 attacctcta ccggttcggc accgaggagc agaagcggcg gtggctcgtc ccggcgatcg
     1201 ccggcgcgaa gatctccgcg ctcgccgtca ccgagcccga cgccggctcc gacgtcgcgg
     1261 gcatccgcac gcgtgccgtc cgcgacggcg acgcgtgggc cctcgcgggg tcgaagacgt
     1321 tcatcacgaa cgggcccgtc gccgacgttc tcgtcgtcgc tgcgaagacc gacccggacg
     1381 ccgcgcacgg cggcatctcg ctgttcgtcg tcgaggcggg gacgcccggc ctcgtcgcgc
     1441 gccgcatcga gaccgtcgga tggcgaacct cgcacaccgg ggaactcgtg ttcgaccggt
     1501 gtcgcgtgcc ggcggagaac gtcctcggcg aggaggggcg cggcttcgtc cagatcatgc
     1561 agagcttcca gtgggagcgg atcgtcatgg cgctctctgc ggtcgccgcc gccgacctca
     1621 cgctcgagat ggcgatcgcg tacgcgcgcg aacgccacgc gttcggacgt cccgtcgcgc
     1681 ggttccaggt gtggcgacac cggttcgccg acctcgcgac cgagatcgag gcggcccgct
     1741 cgctcacgta ccaggccctg cggaagatcg tcgccggcga ggacgccgtt cgcgaggtct
     1801 cgatggcgaa gtggctcggg accgagctcg actggagggt ggcggacgag gccgtgcagg
     1861 tccacggcgg atacggctac atgatggaat tccccgcgca gcgcgcgtgg cgcgacgcgc
     1921 gcctcggcgc gatcggcggc gggaccaccg agatcatgaa agagatcatc ggccgctcgt
     1981 acggcctgtg agcgtcgcgc cgacgtcggc gagcccctcg cgataggtcg ggaagcccgg
     2041 cgcccaacgc agctcgcgct ttaccttcgc gttggagacc cgcagccgcg ttccgcccat
     2101 gaacgtcgcg gcgtacgagg cgaagggcct cgcgagccac accgggaccg acagagcgcg
     2161 ggcggctccg gtcgcccggg agaactcgtc ggtcaacgct cggaacgaga ccggctcgtc
     2221 gtcgacgacg ttgtagaccc gccggcacgg ccacgctcca gcgcggacac ggtggcttcg
     2281 gcggcatcct cgatgtggat ccacgaggcc acgccgcgtc cgccgcccgg gagcgccatc
     2341 atccgtcgtc ttagcagctt ggccatcagc tccgtggatc cgacgccggg gccgtagaag
     2401 agcccgtatc ggaggacgac gccgtccatc ccgccggaga gcacggagcg ctccttcgtg
     2461 tggatctc
//
LOCUS       DASZOB010000187         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_155156, whole genome shotgun sequence.
ACCESSION   DASZOB010000187 DASZOB010000000
VERSION     DASZOB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155156"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="ccrA"
                     /locus_tag="VG709_03090"
     CDS             <1..984
                     /gene="ccrA"
                     /locus_tag="VG709_03090"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWC32092.1"
                     /translation="EFHIGGSDASGIVYAVGARVTNVKVGDEVVIHCAQWDSAGPVGT
                     SGGDPMYDPSFRIWGYESNWGSFAQFTKVQAHQCLPKAPQLTWEAAAAPPLVGATAYR
                     MLFGWPPNVVEEGDPVLVWGGAGGLGSMAIQIVREHGGRAVAVVSSDDKAEFCRKLGA
                     VGVVNRRDFDHWGMPPHWTDTEAYAQWIEKTKAFGKAFFEALGEKRAPKIVFEHPGED
                     TVPTSIFVCDTGGMVVICAGTTGYNAVSDLRYLWMRQKRFQGSHFANDEQSLAFNQMV
                     VDGKVDPALSRTFSFEDIGTAHQLMSENAHPEGNMAALVSAPREGMTGLPD"
     gene            complement(1001..1321)
                     /locus_tag="VG709_03095"
     CDS             complement(1001..1321)
                     /locus_tag="VG709_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC32093.1"
                     /translation="MASARPQETRSFYETLGLEVGMDMGWIATLVSPSNPTAQISVVE
                     GDDPEPQLTVEVDDVDAVYERAREGDAPIVRQIRDEDWGVRRFFVRDPNGLVVNVMGH
                     LGRA"
     gene            complement(1349..1750)
                     /gene="mce"
                     /locus_tag="VG709_03100"
     CDS             complement(1349..1750)
                     /gene="mce"
                     /locus_tag="VG709_03100"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWC32094.1"
                     /translation="MLEDIDHIGIATRDLESAVERYRSAFGVEPVHRETVEDQGVEEV
                     LFKVGTSYIQLLGALGAETPVGKFLEKRGEGTHHVGYRVRDIRATLARLRDEGVQLID
                     EEPRPGSRGTTVAFVHPKAMGGVLVELVEEP"
     gene            1912..3840
                     /locus_tag="VG709_03105"
     CDS             1912..3840
                     /locus_tag="VG709_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC32095.1"
                     /translation="MDESPRARREQAAPQVDSPPRSGTTAGEADVTVTNPVAAFLGQS
                     KLIKAGRADVARVRERTRRRRLLRVAFFLAFIDAFLWYRVAIGDPLGPPSLPDDAMIW
                     LPGMLLVLILALVLLLPMGSGRSPHLLVRPEHIETGLEDVKGLDPQVDEVVRSLNVFL
                     GYSTFRERLGGNPRRGILFEGPPGTGKTYLAKAMAKQAGVPFLYVSAPAFQSMWFGMT
                     GARIRSFFRALRKLARREGGAIGFIEEIDAIGTDRAAGAAMSPADAPGRAVSRMVSPG
                     TGGMVNELLIQMQSFDQPPWTTRARDRFVEWFNGYLPAGKRIRTRRPEYHNILLIAAT
                     NRADTLDPALLRPGRFDRRLYFDAPTKKGRRELIDYFLDRKSHEPDLDDEAARERLAH
                     DTLGYTPVMVEHLFDEALLIALRNGRDRMTVADVYEAKLSDEIGLKQPVVYTEAARRA
                     VATHEAGHATAAYILGTTRRLEVLSIIKRRQSLGLLAHADLEERFTKSRSELEAALAI
                     SLAGMAAEELFLGESGTGPASDLTAATEVAAAMVGALGMGGSLVSYEAIAEGPVSMKN
                     LVGKVLADGEGKARVENLLAAQKERSVAVLSENRDVVEALRDALMERDELVGNEITAV
                     IKEALARRGLPAVPDVNV"
     gene            complement(3837..4934)
                     /locus_tag="VG709_03110"
     CDS             complement(3837..4934)
                     /locus_tag="VG709_03110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWC32096.1"
                     /translation="MATTEVRIDPAIWEEPVRGGYPDPRIFGLSGIEQMRAYFRGFAP
                     APPLGRLTGLLPTHVGPGAATFVMPATEWLLTPAPYISLGVQAMLADAALGCAIQTTL
                     PPATPYTTSDLSLSFLRPLEADGGQVAARGRLVHSGRSLGVAELVIEDSHARPVTHGT
                     TRCFFLDPLDVPAPDLGELPRFDGPPFADDDPFRRPVAGTSLPQDAWDRMTGLDVMRA
                     LIAGELPAPPITHLTGVRPIEVGEGTAAFVLPSSGWLTSPIGTVQGGATAMLAETVLA
                     TAVQTTCAPRTAYAPLDVKVNYLRPVFADGRDIVGRAHVVHRGKTLGVARADLVNADG
                     KTVAVATGTTLIREGTPWQPEEPAAPGAAEA"
     gene            complement(4921..>5366)
                     /locus_tag="VG709_03115"
     CDS             complement(4921..>5366)
                     /locus_tag="VG709_03115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWC32097.1"
                     /translation="STFPVGELFGFRGILEEEGKSSFSMPASQWFCAAGGTIYGGALA
                     VLADAVMGGAVANTLPPKSSYATLDLKVHFLRPPQPDGRDLVATATVVHRGRRMAVAT
                     GDIVDADGKKIAMATSSHMIFEGRSWVREQATEALDEQAHDDGDD"
BASE COUNT          797 a         1880 c         1914 g          775 t
ORIGIN      
        1 gagttccaca tcggcggctc ggacgcgtcc gggatcgtct atgcggtcgg cgcccgcgtc
       61 acgaacgtca aggttggcga cgaggtcgtc atccactgcg cccagtggga ctccgccggg
      121 ccggtcggga cgtcgggcgg cgacccgatg tacgacccgt cgttccggat ctggggctac
      181 gagtcgaact ggggctcgtt cgcgcagttc acgaaggtgc aagcgcacca gtgcctgccg
      241 aaggcgccgc agctcacgtg ggaggcggcg gcggcccccc ccctcgtcgg cgcgacggcg
      301 taccgcatgc tcttcgggtg gccgccgaac gtggtcgagg agggagatcc cgtcctcgtc
      361 tggggcggcg ccggggggct cgggtcgatg gcgatccaga tcgtgcgtga gcacggtggc
      421 cgcgccgtcg ccgtcgtctc gagcgacgac aaggccgagt tctgcaggaa gctcggcgcc
      481 gtcggcgtcg tcaaccgccg ggacttcgac cactggggta tgccgccgca ctggacggac
      541 accgaggcgt acgcgcagtg gatcgagaag acgaaggcct tcgggaaggc gttcttcgag
      601 gcgctcggcg agaagcgggc cccgaagatc gtgttcgagc accccggcga ggacaccgtt
      661 ccgacatcga tcttcgtctg cgacaccggc ggcatggtcg tgatctgcgc cggcacgacg
      721 ggctacaacg cggtgagcga cctccgatac ctgtggatgc gccagaagcg cttccagggg
      781 tcgcacttcg cgaacgacga gcaatcgctc gcgttcaacc agatggtggt cgacggcaag
      841 gtcgacccgg cgctatcgcg gacgttctcg ttcgaggaca tcggcaccgc gcaccagctg
      901 atgtccgaga acgcgcaccc cgaggggaac atggcggccc tcgtcagcgc cccgcgcgag
      961 gggatgaccg gcttgccgga ctaacgaggg cgcgtggacc tcaggcgcgc ccgaggtggc
     1021 ccatcacgtt cacgacgagg ccgttcgggt cgcggacgaa gaacctgcgg acgccccagt
     1081 cctcgtcgcg gatctgccgc acgatcggcg catccccttc gcgcgcgcgc tcgtagacgg
     1141 cgtcgacgtc gtccacctcg acggtcagct gcggctcggg atcgtcgccc tcgaccacgc
     1201 tgatctgcgc ggtggggttc gagggcgaca cgagcgtcgc gatccatccc atgtccatgc
     1261 cgacctcgag gccgagcgtc tcgtagaacg accgcgtctc ctgcggacga gccgacgcga
     1321 tgttcgggac gaccctcctg acgtcaccct acggctcctc gacgagctcg acgaggacgc
     1381 cacccatcgc cttggggtgg acgaacgcga ccgtcgtccc gcgtgagccg gggcgcggtt
     1441 cctcgtcgat gagctggacg ccctcgtccc tcaaccgcgc gagggtcgcg cggatgtcgc
     1501 gcacgcggta cccgacgtgg tgcgtcccct cgccgcgctt ctcgaggaac ttgccgacgg
     1561 gcgtctcggc cccgagagct ccgagcagct ggatgtagga cgtcccgacc ttgaagagca
     1621 cctcctcgac accctggtcc tcgacggtct cgcggtggac cggctcgacg ccgaacgccg
     1681 accggtaccg ctcgaccgcc gactccaggt ctcgtgtcgc gatgccgatg tggtcgatat
     1741 cctccagcaa cgtccgctcc tcgagggggg gcgcgcagcg gcccatccta cggttgctcg
     1801 ccgagcctgc gccacgcggc aggagatgcg ctatccgcac gtcgaactag actgccgagt
     1861 gcccgatccc cgaacggggg acgggcttct ctccgcaggg agggagcaac cgtggacgaa
     1921 tcgccgcgcg cccgccgcga acaggcagcg ccgcaggtcg actcgccgcc acgaagcggc
     1981 acgacggcgg gtgaggcgga cgtgaccgtc acgaaccccg tggcggcgtt cctcgggcag
     2041 tcgaagctga tcaaggccgg ccgcgccgac gtcgcgcgcg tgcgcgagcg gacgcgccgc
     2101 aggcggctcc tccgcgtcgc gttcttcctc gcgttcatcg acgcgttcct gtggtaccgc
     2161 gtcgcgatcg gcgacccgct cggcccaccg agcctccccg acgacgcgat gatctggctg
     2221 ccggggatgc tcctcgtcct gatcctggcg ctcgtgctcc tcctcccgat ggggagcggt
     2281 cgctcgccgc acctgctcgt tcggcccgag cacatcgaga cggggctcga ggacgtgaag
     2341 ggcctcgacc ctcaggtcga cgaggtcgtg cgcagcctta acgtgttcct cgggtactcg
     2401 acgttccgcg agcggctcgg cgggaacccg cgccgcggca tccttttcga gggccctccg
     2461 ggaaccggca agacgtacct cgcgaaggcg atggccaagc aggccggcgt gccgttcctc
     2521 tacgtctccg cgccggcgtt ccagtcgatg tggttcggga tgacgggcgc gcggatccgg
     2581 tcgttcttcc gggcgctgcg caagctcgcc cgccgggagg gcggcgccat cggtttcatc
     2641 gaggagatcg acgcgatcgg caccgaccgc gcggcgggcg cggcgatgtc gccggccgac
     2701 gccccgggac gggcggtctc gcggatggtc tcgccgggca ccggcgggat ggtgaacgag
     2761 ctcctgatcc agatgcagtc gttcgaccag ccgccgtgga ccacccgagc gcgcgaccgc
     2821 ttcgtcgagt ggttcaacgg gtacctcccc gcgggaaagc ggatccgcac tcgccgcccc
     2881 gagtaccaca acatcctgct gatcgcggcg acgaaccgcg cggacacgct cgacccggcg
     2941 ctgctccgtc cgggccggtt cgaccggcga ctgtacttcg acgcgccgac gaagaagggg
     3001 cggcgcgagc tgatcgacta cttcctcgac cgaaagtcgc acgagcccga cctcgacgac
     3061 gaggcggcgc gcgaacggct cgcgcacgac acgctcggct acacgccggt gatggtcgag
     3121 caccttttcg acgaggcgtt gctcatcgcg ctccggaacg ggcgtgatcg catgacggtc
     3181 gccgacgtgt acgaggcgaa gctctcggac gagatcggcc tcaagcagcc cgtcgtctac
     3241 acggaagccg cgcgccgagc cgtcgcgaca cacgaggccg gccacgcgac cgcggcgtac
     3301 atcctcggga cgacgcgccg gcttgaggtg ctctcgatca tcaagcgccg gcaatcgctc
     3361 gggctcctcg cgcacgccga tctcgaagag cggttcacga agtcgcgctc ggagctcgag
     3421 gccgcgctgg cgatctcgct cgcggggatg gcggccgagg agctcttcct cggcgagagc
     3481 gggaccgggc ccgcgtcgga ccttacggcg gcgaccgagg tcgcggcggc gatggtcggc
     3541 gcgctcggga tggggggatc gctcgtgtcc tacgaggcga tcgcggaggg tccggtctcg
     3601 atgaagaacc tcgtggggaa ggtcctcgcc gacggcgagg ggaaggctcg ggtcgagaac
     3661 ctcctcgcgg ctcagaaaga gcgctcggtc gcggtgctct cggagaaccg cgacgtcgtc
     3721 gaggcgctgc gcgacgcgct gatggagcgc gacgagctcg tcggcaacga gatcaccgcg
     3781 gtgatcaagg aggcgctagc gcgccgcggg ctgccggcgg tgccggacgt aaacgtctag
     3841 gcctccgcgg cgcccggggc ggccggctct tcgggctgcc atggcgtccc ttcgcggatc
     3901 agcgtcgtcc cggtcgcgac cgccacggtc ttgccgtcgg cgttgacgag atcggctcgc
     3961 gcgacaccga gggtcttccc gcggtgcacg acatgcgctc gtccgacgat gtcgcgcccg
     4021 tccgcgaaga ccgggcggag gtagttcacc ttcacgtcca gcggcgcgta cgccgtccgt
     4081 ggcgcgcacg tcgtctggac ggccgtcgcg aggacggtct cggccagcat cgcggtcgcg
     4141 ccgccctgca cggtcccgat cgggctcgtg agccagccgc tcgacggaag cacgaacgcg
     4201 gccgttccct ccccgacctc gatcggacga acccccgtga ggtgcgtgat cggcggcgcc
     4261 ggaagctccc cggcgatcag cgcccgcatg acgtcgagcc ccgtcatgcg atcccaggcg
     4321 tcctggggga gcgacgtccc cgcgaccggg cggcggaagg ggtcgtcgtc ggcgaacggc
     4381 ggcccgtcga agcgcggcag ctcgccgaga tccggtgctg ggacgtcgag cggatcgagg
     4441 aagaagcagc gggtcgtccc gtgcgtcacc gggcgcgcgt gggagtcctc gatcacgagc
     4501 tcggcgacgc cgagggatcg tccgctgtgc acaagccgac cgcgtgccgc gacttgaccg
     4561 ccgtcggctt cgagcgggcg caggaacgaa agcgagagat ccgaagtggt gtacggcgtc
     4621 gccggcggca gcgtggtctg gatcgcgcaa ccgagcgccg cgtcggcgag catcgcctgc
     4681 acgccgagcg agatgtacgg ggccggcgtg agcagccact ccgtcgccgg catcacgaac
     4741 gtcgcggcgc cggggccgac gtgcgtcgga agcagtcccg tgagccggcc caggggcggc
     4801 gccggggcga acccgcggaa gtacgcacgc atctgctcga tgcctgacag gccgaagatc
     4861 cgtggatccg ggtagccgcc gcggacgggc tcctcccaga tcgcgggatc tatccggacc
     4921 tcagtcgtcg ccatcatcgt gcgcctgctc gtccagcgcc tcggtcgcct gctcacgcac
     4981 ccacgacctt ccctcgaaga tcatgtggct gctcgtggcc atcgcgatct tcttgccgtc
     5041 cgcgtcgacg atgtcgccgg tcgcgaccgc catgcggcgc ccgcgatgga cgacggtcgc
     5101 ggtggcgacg aggtcgcgcc cgtcgggctg tggcggacgc aggaagtgca ccttgaggtc
     5161 gagcgtcgca taggagctct tcggaggcag cgtattcgcg acggccccgc ccatgaccgc
     5221 gtccgcgagc accgcgagcg cgccgccgta gatcgtgccg ccggccgcgc agaaccactg
     5281 gctcgccggc atcgagaacg acgactttcc ctcctcctcg aggatgcccc ggaagccgaa
     5341 gagctcgccg acggggaacg tcgagt
//
LOCUS       DASZOB010000188         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_12127, whole genome shotgun sequence.
ACCESSION   DASZOB010000188 DASZOB010000000
VERSION     DASZOB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12127"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1033)
                     /locus_tag="VG709_03120"
     CDS             complement(47..1033)
                     /locus_tag="VG709_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HWC32098.1"
                     /translation="MQTITPVVHGGRRSRWGASVAAHAVGATASSGALGAARGAAGRG
                     RGAPWGATGLVLVTGLAVLYAAREASALPVPVPELRRQVPEWWRSSFSPPVASLLYGI
                     GLGVGFATHLRHGTFVVVGAAALASGDPAVGAALLAPFGLARALTVATAMGSTTAERV
                     ARASERLERWAMGAAPRLANGAVLVAVVAGAVVVLSSGSVAREGSSGMAPFVVASVLA
                     WAAMAKALRFRAWRDSLGAHGIEGPVAGAVSFAVPTAEAATVALFVVDVRVGAATAIA
                     LLVAFTLALLRASRISGRRLPCGCFGRTKTRDVGLLVGRNVLLIGLSALMLA"
     gene            complement(1117..1644)
                     /locus_tag="VG709_03125"
     CDS             complement(1117..1644)
                     /locus_tag="VG709_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32099.1"
                     /translation="MSAALESAGRALASRTSRRSFLGRVGKAFLALTGGAFVGVALSP
                     SGAEAYHICGHIYTTGSCPHPYAPQTRIDRYGYPVHPVYGYPVADNGDIYTSRRQRRR
                     KVCEQVVASRYPYTRPAVLQGVWSRCCSGRIRRVLDCCSYSDTRINGDASLTGYCYQG
                     RKVFCITYIDTKVRC"
     gene            complement(1641..2195)
                     /locus_tag="VG709_03130"
     CDS             complement(1641..2195)
                     /locus_tag="VG709_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32100.1"
                     /translation="MSGWWAVSYVVLWVLVVVLGILVVALAREVGTLHLRRGPRGALE
                     LDEEGPPLGEAPEPIDALDSEGRHVTVGGPGGWQLLVFVSPGCPVCREVLPSIEPVAR
                     VEKLRAVVVSDERDVPVVGGAAVVPAPDIAERYATPGTPYVVLLDAMGVVRAKGTVNN
                     LEQIEGLVRTAKKRLEIELAGGGA"
     gene            2215..>2781
                     /locus_tag="VG709_03135"
     CDS             2215..>2781
                     /locus_tag="VG709_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HWC32101.1"
                     /translation="MPTVGAILALLSFALQPAATSATQADPADLRVTKRDARDPVDAG
                     RVLAYVVAVANDGPDPAADVTLTDTLPADVVFHDVTASQGSCTGGTPQVVCELGTLRS
                     GGTATVRIRVVPTRQGTISNTATASSTTTDPDPSDNTVAEPTTVNGPSCTRVGTAADD
                     RLSGTSSSDVLCGAGGAVVLVPGGGSDVV"
BASE COUNT          444 a         1034 c          989 g          314 t
ORIGIN      
        1 cgtcggcacc ggtcggcacg ccgatcaagg ggagcgagac cgggggctag gcgagcatga
       61 gcgccgagag gccgatcagg aggacgttcc gaccgacgag cagcccgacg tctcgcgtct
      121 tcgtgcggcc gaagcagccg caggggaggc gtcgcccgga gattcggctc gcgcgcagca
      181 acgcgagcgt gaacgccacg agcagcgcga tcgcggtcgc cgcgcccacc cgaacgtcga
      241 cgacgaacag cgcgacggtg gcggcctcgg cggtcggcac cgcgaacgag acggcgcccg
      301 cgacgggtcc ttcgatcccg tgggcgccga ggctgtcccg ccatgcgcgg aaacggagcg
      361 ccttggccat agctgcccag gccagcacgc tcgcgacgac gaacggcgcc atgccggagc
      421 tgccctcgcg cgcgacggag ccggaactga ggacgacgac agcgccggca accacggcga
      481 cgagcaccgc gccattcgcg agtcgcggcg ccgcacccat cgcccaccgc tcgagccgct
      541 ccgacgcgcg agcaacgcgt tccgccgtcg tcgatcccat cgcggtcgcg acggtcaacg
      601 cgcgggcgag cccgaacggc gcgagcagcg cggcgcccac cgccgggtca cccgaagcga
      661 gcgctgcggc tccgacgacc acgaacgtgc cgtgacggag gtgggtcgcg aagccgacgc
      721 cgagaccgat cccgtagagc agcgaggcga cgggcgggct gaaggagctc cgccaccatt
      781 ccgggacctg cctccggagc tccgggaccg ggacggggag agcggacgcc tcgcgggctg
      841 cgtagaggac cgcaagaccc gtcaccagaa cgaggccggt ggcgccccac ggcgcgccgc
      901 gcccccgccc ggccgcgccg cgcgccgctc cgagcgcacc ggacgaggcg gtcgcgccga
      961 cggcgtgcgc cgcgaccgac gccccccatc tcgaccttcg ccctccgtgg accacggggg
     1021 tgatggtctg caccatcgac agccctcagg ggctgaagag cgtcgatagt cccgctacga
     1081 ccgacgcgac gacgaccggt acggcgacgg ccgggttcag cagcgcacct tggtgtcgat
     1141 gtacgtgatg cagaacacct tgcggccctg gtagcagtag ccggtcagcg acgcgtcgcc
     1201 gttgatgcgc gtgtccgagt acgagcagca gtcgaggacg cgacggatcc tccccgagca
     1261 gcaccggctc cagacgccct gcagcactgc cgggcgcgtg tacgggtacc gcgacgcgac
     1321 cacctgctcg cagaccttcc gccgtcgctg cctccgcgac gtgtagatgt cgccgttgtc
     1381 ggcgacggga tacccgtaca cggggtgcac gggatacccg tagcggtcga tgcgcgtctg
     1441 cggcgcgtac gggtgcggac acgatcccgt cgtgtagatg tggccgcaga tgtggtacgc
     1501 ctcggcaccc gacggcgaga gcgcgacgcc gacgaaggcg ccgccggtga gcgcgaggaa
     1561 cgccttcccg acgcggccga ggaacgaccg ccggctcgtc ctcgatgcga gcgcgcgccc
     1621 ggcgctctcg agggcggcgc tcacgcgcca cctcccgcga gctcgatctc gaggcgcttc
     1681 ttcgcggtcc tcacgagccc ctcgatctgc tcgaggttgt tgacggttcc cttggcgcgg
     1741 accacgccca tcgcgtcgag gagcacgacg tacggcgttc cgggggtcgc gtagcgctcg
     1801 gcgatgtcgg gggccgggac gaccgcggcc cctccgacca caggaacgtc gcgctcgtcc
     1861 gagaccacga cggcacggag cttctccacg cgcgcgaccg gttcgatcga gggcagcacc
     1921 tcgcggcaca ccgggcaccc gggcgagacg aagacgagga gctgccagcc gccgggacct
     1981 cctacggtga cgtggcgccc ttcggagtcg agcgcgtcga tcggctcggg cgcctctccg
     2041 agcggaggcc cttcctcgtc gagctcgagg gcgccacgcg ggccgcggcg gaggtggagc
     2101 gtcccaacct cacgcgcgag cgcgacgacg aggatgccga gcaccacgac gaggacccac
     2161 agcaccacgt acgagaccgc ccaccagccc gacattgagc tagcattctc ggccgtgccc
     2221 acggtcggcg caatcctggc gctcctgtcg ttcgcgctcc aaccggccgc gacctcggcg
     2281 acgcaggcgg accccgcgga cctgcgcgtc acgaagcgcg acgcccgcga tcccgtcgac
     2341 gccgggaggg tcctcgcgta cgtcgtcgcg gtcgcgaacg acggcccgga tccggcggcg
     2401 gacgtcacgc tgaccgacac gctcccggcg gacgtcgtct tccacgacgt aaccgcgagc
     2461 cagggttcgt gcaccggcgg gacgccacag gtcgtgtgcg agctcgggac gcttcgctcc
     2521 ggcggcaccg caaccgtgcg catccgcgtc gtcccgacac gacaggggac gatcagcaac
     2581 acggcaacgg cgagcagcac gacgaccgac cccgatcctt ccgacaacac cgtcgcggag
     2641 ccaacgacgg tgaacgggcc gagctgcacg agggtaggaa cggccgccga cgaccgcctc
     2701 tccggcacgt cgagctccga cgtgctctgc ggcgccggag gcgccgtcgt ccttgtgccc
     2761 gggggcggat ccgacgtcgt c
//
LOCUS       DASZOB010000189         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_54335, whole genome shotgun sequence.
ACCESSION   DASZOB010000189 DASZOB010000000
VERSION     DASZOB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54335"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..342)
                     /locus_tag="VG709_03140"
     CDS             complement(43..342)
                     /locus_tag="VG709_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32102.1"
                     /translation="MSPLKQLVVALAAMSLAACGGNGGGEDYPPEVVQQFMSSCTEGG
                     TSEAVCQCALDKFEAKYSAEEFEEHGQKLAAGEPDEEFTQDLFAYSAECAQEDAN"
     gene            437..718
                     /locus_tag="VG709_03145"
     CDS             437..718
                     /locus_tag="VG709_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32103.1"
                     /translation="MAVKVPNDFREFAMLRQDVEAVIQRCGKDTFDCILIDLEGNWTR
                     AVFPSEDAAREVCTDLGVRSVVGWEDPRMARRMNRRDHWGEPGGQRRAL"
     gene            799..1260
                     /gene="rimP"
                     /locus_tag="VG709_03150"
     CDS             799..1260
                     /gene="rimP"
                     /locus_tag="VG709_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWC32104.1"
                     /translation="MDLEPLVRPVVEASGLELVDVAFRRESGRRVLRVTVDREGGVDL
                     DAIADTSGRISRRLDVEGFDPGPYDLEVSSPGVERPLRSVEDFARRIGEKVKVKTHEP
                     VDGARTHVGTISSVGGSSVMLEIDEREREIPADAISSARTVFEWGQARSKP"
     gene            1257..>2440
                     /gene="nusA"
                     /locus_tag="VG709_03155"
     CDS             1257..>2440
                     /gene="nusA"
                     /locus_tag="VG709_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWC32105.1"
                     /translation="MSEDLVQALKELEREKGISFDTILHGLEEALASAYRSQIRGHAA
                     ADEEEDDREEIRVTLDPGTGDMWATVRQMDEEGNVVSQEKHELSDDFKGRIAAQTAKQ
                     VILQKLRDAEREITYEEFAGREGDVVTGIVQQDSHRYTLLDLGKVEALLPVQEQVPAE
                     QYRHGERLKAYLVEVRKTTKGPQIIVSRTHPELLKKLFELEVPEIAEGIVEIKAVARE
                     PGHRSKIAVSSNEPGVDPVGACVGAKGSRVRMVVNELKGEKIDVVEWSDDTARFVGKA
                     LAPASVKEVRIDRDSGTALVIVPDYQLSLAIGKEGQNARLAARLTGWRIDIKSEGQFA
                     EEQAAARRAPKAPATDAPRDAAPAEPAREATSTDKSAAEEPAAEEPVADEPRDDAEGL
                     ASG"
BASE COUNT          408 a          801 c          888 g          343 t
ORIGIN      
        1 gcggacgccg tggtcgaggc gctgtccgaa gtcccctgac ggctagttcg cgtcctcctg
       61 cgcgcactcg gcgctgtacg cgaacaggtc ctgtgtgaac tcctcgtctg gttcgccggc
      121 cgcgagtttc tggccgtgct cctcgaactc ctccgcggag tacttcgcct cgaacttgtc
      181 gagggcgcac tggcagaccg cctcggacgt cccgccttcg gtacacgacg acatgaactg
      241 ctggacgacc tcgggcggat aatcctcgcc gccgccgttt ccgccgcagg ccgcgagcga
      301 catcgcggcg agcgcgacca cgagctgctt caacgggctc atgcctacct ccctcgaatt
      361 ggctcgcgca accgtagggg gcgacctcgc gcgaggcaac ttgtcggccg ttcgttacgg
      421 ccggtagcct gcgcgcgtgg cggtcaaggt gccgaacgac ttccgtgagt tcgcgatgct
      481 gcgccaggac gtcgaggccg tgatccagcg gtgcgggaag gacacgttcg actgcatcct
      541 gatcgacctc gagggcaact ggacccgcgc cgtcttcccc tccgaggatg ccgcgcgcga
      601 ggtctgcacc gacctcggag tccggtcggt cgtcgggtgg gaggacccgc ggatggccag
      661 gcggatgaac cgccgcgatc actggggcga gcccggcggc cagcgccggg cgctctagct
      721 ctcggatcgg ccgctctccc gcaggcgcta tactcggatg cagcgcgtgg gcgagggccc
      781 accttttttt tgggagcgat ggacctggag ccgctggtga gaccggtcgt cgaggcttcg
      841 ggcctcgagc tcgtggacgt ggcgttccgc cgcgagagcg gccggcgcgt cctgcgcgtg
      901 accgtcgacc gcgaaggtgg ggtcgacctc gacgcgatcg ccgacacgag tgggcggatc
      961 tcccggcggc tcgacgtcga ggggttcgac ccaggtccgt acgacctcga ggtgagctcg
     1021 cccggcgtcg agcgcccgct acggtcggtt gaggacttcg cgcgacggat cggcgagaag
     1081 gtaaaggtga agacccacga gccggtcgac ggtgcgcgca cgcacgtcgg gacgatctcg
     1141 tcggtcggcg ggtcgagcgt gatgctcgag atcgacgagc gcgagcgcga gattcccgct
     1201 gacgcgatct cgtccgcgag aacggttttc gagtggggac aggcgaggag caagccgtga
     1261 gcgaagatct ggtgcaggcc ctgaaggagc tcgaacgaga gaagggcatc tcgttcgaca
     1321 cgatcctgca cgggctggag gaggcgctgg cgtccgcgta caggtcccag atccgcggcc
     1381 acgccgccgc ggacgaggaa gaggacgatc gcgaggagat ccgggtgacg ctcgacccgg
     1441 gtacggggga catgtgggcg acggttcgac agatggacga ggagggcaac gtcgtctcgc
     1501 aggagaagca cgagctctcc gacgacttca aggggcgcat cgccgcgcag accgccaagc
     1561 aggtgatcct ccagaagctc cgcgacgccg agcgcgagat cacctacgag gagttcgccg
     1621 ggcgcgaggg cgacgtcgtc acaggcatcg tccagcagga ttcgcaccgg tacacgctgc
     1681 tcgatctcgg caaggtcgag gcgctgctac cggtccagga gcaggtcccc gccgagcagt
     1741 accgccacgg cgagcggctc aaggcgtacc tcgtcgaagt ccgcaagacc acgaaaggac
     1801 cgcagatcat cgtctcgagg acgcacccgg agctcctcaa gaagctgttt gagctcgagg
     1861 ttcccgagat cgccgagggc atcgtcgaga tcaaggccgt cgcgcgagag ccggggcatc
     1921 gctcgaagat cgcggtctcg tcgaacgagc cgggcgtcga ccccgtcggc gcctgcgtcg
     1981 gcgcgaaggg ctcgcgcgtc cggatggtgg tgaacgagct caagggcgag aagatcgacg
     2041 tcgtcgagtg gtccgacgac acggcgcggt tcgtcggcaa ggcgctcgcg ccggcttccg
     2101 tcaaggaggt acgcatcgac cgcgacagcg ggacggccct cgtgatcgtc cccgactacc
     2161 agctctctct cgcgatcggc aaggaggggc agaacgcgcg actcgcggcc cggctgaccg
     2221 gctggcgcat cgacatcaag agcgagggcc agttcgccga ggagcaggcg gcggcccgtc
     2281 gcgcgccgaa ggcaccggcg accgacgcgc cgcgggacgc agcacccgcc gagccggcgc
     2341 gcgaggcgac gtccaccgac aagtcggcgg ccgaagagcc ggcggccgaa gagcccgtcg
     2401 cggacgagcc cagggacgac gccgaggggc tggcctccgg
//
LOCUS       DASZOB010000190         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_145152, whole genome shotgun sequence.
ACCESSION   DASZOB010000190 DASZOB010000000
VERSION     DASZOB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145152"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VG709_03160"
     CDS             complement(<1..510)
                     /locus_tag="VG709_03160"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HWC32106.1"
                     /translation="MRPVLTAEEARSLEREAERRGTSVELLMERAGRALAHAAARLAD
                     GSYGKRAVIVAGRGNNGGDGLVAARHLSRAGRGVTVLLVGDPDGMPEPAATNLRRVRD
                     SGVRLMPFAPDAAARAFEAADVAVDALLGLGLRGAPRGPSASAIASFDGHDLPVVAAD
                     IASGVEADTG"
     gene            complement(512..883)
                     /gene="acpS"
                     /locus_tag="VG709_03165"
     CDS             complement(512..883)
                     /gene="acpS"
                     /locus_tag="VG709_03165"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWC32107.1"
                     /translation="MRVVGIGVDLVEVERVRDLLERRPGFREEVFTASELADCAEVDD
                     VACLAARWAAREAAVKALGGVRDAGWRDVVVERDDSGRPTIALSGAARERAREAGVDH
                     VLVSLSHERSVAAAFCLAVGA"
     gene            complement(893..>1537)
                     /locus_tag="VG709_03170"
     CDS             complement(893..>1537)
                     /locus_tag="VG709_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWC32108.1"
                     /translation="EVCVAATKPFATQMVSLHLLALFLAQVRETMPTAEIGKVVDRIL
                     ELPSQVKRALELDAPISDLAERYHEARDILFIGRHTGYPAALEGALKLKELSYIHAEG
                     YPAGELKHGPIALIEPGLPVVAIATQSHVYPKVLSNIQEIKARGANVIAIASAGDTEI
                     AKSADHVIEVPKTPELLAPVVVSVPLQLLAYRIAKLRGCDVDQPRNLAKSVTVE"
BASE COUNT          221 a          580 c          533 g          203 t
ORIGIN      
        1 gccggtatcg gcctcgaccc cggacgcgat gtccgcggcg acgacgggga ggtcgtgccc
       61 gtcgaacgac gcgatcgccg acgcggacgg gcccctcggc gcgccgcgca ggccgaggcc
      121 gaggagcgcg tcgaccgcga cgtctgcagc ctcgaaggct cgcgccgcgg cgtcgggagc
      181 gaacggcatc agccgaacgc cggagtcacg cacccggcgc agattcgtgg cggcgggctc
      241 cggcatccca tcggggtccc cgacgaggag aacggtcacc cccctcccgg ccctcgagag
      301 atggcgggcg gcgaccagcc cgtcgccccc gttgttgcct cgccccgcga cgatcaccgc
      361 ccgcttgccg tacgacccgt ccgcgagccg cgcggcggcg tgcgccagcg ccctcccggc
      421 gcgctccatg aggagctcga cggaggtccc gcggcgctcc gcctcgcgtt cgagggatcg
      481 cgcctcctcg gcggtcagca ccggccgcat gtcaggctcc caccgcgaga cagaatgccg
      541 cggcgacgga acgttcgtgc gacagcgaca cgaggacgtg atcgacgccg gcctcccggg
      601 cgcgctccct cgcggcgccc gagagcgcaa tcgtcggccg acccgagtcg tcgcgctcga
      661 cgacgacgtc tcgccacccc gcatcgcgca ccccgccgag ggccttcacc gcagcctcac
      721 gggccgccca tcgggcggcg agacacgcga cgtcgtcgac ctccgcacag tcggccaact
      781 cggacgcggt gaacacctcc tcacggaatc ccggtcggcg ctcgaggaga tcccgcacac
      841 gctccacctc gaccaggtcc acgccgatgc cgacgacccg cacggggccc gcctactcga
      901 cggtgaccga cttcgcgagg ttgcgcggct gatcgacgtc gcatccgcgc agcttcgcga
      961 tccggtacgc gaggagctgg agcgggaccg agacgacgac cggcgccagc agctcggggg
     1021 tcttcggcac ctcgatcacg tggtcggcgg acttcgcgat ctcggtgtcg cccgcgctcg
     1081 cgatggcgat gacgttcgcg ccgcgcgcct tgatctcctg gatgttcgag agcaccttcg
     1141 ggtagacgtg gctctgggtg gcgatcgcca ccaccgggag cccgggctcg atcaacgcga
     1201 tcggcccgtg cttcagctcc cccgccggat acccctcggc gtggatgtac gacagctcct
     1261 tgagcttcag cgcgccctcg agggcggccg ggtagccggt gtgccgtccg atgaagagga
     1321 tgtcgcgcgc ctcgtgatag cgctcggcga ggtcggagat cggcgcgtcg agctccaggg
     1381 cgcgcttcac ctggctgggc aactcgagga tgcgatcgac caccttgccg atctccgcgg
     1441 tcggcatcgt ctcccggacc tgggcgagga acagcgcgag caggtgcagc gagaccatct
     1501 gtgtcgcgaa cggcttcgtg gcggcgacgc agacctc
//
LOCUS       DASZOB010000191         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_105293, whole genome shotgun sequence.
ACCESSION   DASZOB010000191 DASZOB010000000
VERSION     DASZOB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105293"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..653
                     /gene="pyrR"
                     /locus_tag="VG709_03175"
     CDS             39..653
                     /gene="pyrR"
                     /locus_tag="VG709_03175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWC32109.1"
                     /translation="MRLRRDPVRPFRFLASRELAGGAVPFEPRARLLAGEEIRRAVTR
                     IAHEIVERNRGAEDVVLVGVAARGDDIAGRLHDEIARIEGVDVPVGVLDITFYRDDIG
                     MRAEAPEVHETRIPFDVTGKVVVLVDDGLFTGRTVRAAMDALVDFGRPRAVQLAVLVD
                     RGHRELPIRADYVGKNVPTRVDELVRVRTQETDGVDAVEVGEER"
     gene            650..1567
                     /locus_tag="VG709_03180"
     CDS             650..1567
                     /locus_tag="VG709_03180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWC32110.1"
                     /translation="MKGNLLSVTQLSAEDITRILDTADSFREVGTRVIKKVPALRGRT
                     VVNLFYEASPRTRISFELAAKRLSADVINFTTSGSSVSKGESLKDTALTLQAMGADAI
                     VVRHSSSGAPNALSRWVDASVINAGDGTHEHPTQALLDLYTMREHFPSLEGLRVAIVG
                     DVLHSRVARSGTHALARMGAQVTLVGPPTLIPPDAPAWGAEVSYELDPVLPKANVCYM
                     LRVQRERQRQQFFPSVREYSRRFGLTRDRVAMLPEGALIMHPGPMNRGVEIASEVADL
                     PQSLITDQVTNGIAVRMALLFLLLGGEAA"
BASE COUNT          239 a          519 c          597 g          239 t
ORIGIN      
        1 ggcgaggccg ggaagggagg accagagctt gaggacgcat gcggctgcgc cgggacccgg
       61 tgcggccgtt tcgtttcctg gcctcccgcg agcttgcggg gggcgcggtg ccgttcgagc
      121 ctagggcgag gctcctcgcc ggcgaggaga tccggcgggc ggtcacgcgc atcgcccacg
      181 agatcgtcga gcggaaccga ggcgccgaag acgtcgtgct cgtcggggtc gcggctcgcg
      241 gcgacgacat cgccgggcgg cttcacgacg agatcgcgcg gatcgagggc gtcgacgtac
      301 cggtcggagt cctcgacatc accttctatc gagacgacat cgggatgcgg gccgaggctc
      361 ccgaggtcca cgaaacccgc atcccgttcg acgtgaccgg caaggtcgtg gtgctcgtcg
      421 acgacggcct gttcaccggg aggaccgtcc gggccgcgat ggacgcgctg gtcgatttcg
      481 gaaggccgcg cgcggtgcag ctcgcggtgc tcgtcgaccg cggccatcgc gagctcccga
      541 tcagggccga ctacgtcggc aagaacgtcc cgacccgcgt ggatgagctg gtgcgtgttc
      601 gcacgcagga gaccgacggc gtcgacgccg tcgaggtggg ggaggagcga tgaaagggaa
      661 cctgctctcg gtgacgcagc tgtctgccga ggacatcacg aggatcctcg acacggccga
      721 ctcgttccgc gaggtcggca cccgcgtcat caagaaggtg ccggcgctcc gagggaggac
      781 ggtcgtcaac ctcttctacg aggcgtcgcc gcggacgcgc atctctttcg agctcgctgc
      841 gaaacgtctg tcggccgacg tcatcaactt cacgacgtcg ggctcgtcgg tgagcaaggg
      901 cgagagcctc aaggacaccg cgctgacgct gcaggcgatg ggggcagacg cgatcgtcgt
      961 gaggcactcg tcgtcgggtg cgccgaacgc gctgtcgcgc tgggtcgatg cgagcgtgat
     1021 caacgcgggc gacggcacgc acgagcaccc gacgcaggcg ctgctcgacc tttacacgat
     1081 gcgggagcac ttcccgtcgc tcgaggggct gcgcgtcgcg atcgtcggcg acgtgctgca
     1141 ctcgcgcgtc gcgcgttcgg ggacgcacgc gctcgcgcgg atgggcgcgc aggtgacgct
     1201 cgtcggcccg ccgacgctga ttccgcccga tgcgccggcc tggggagcgg aggtctcgta
     1261 cgagctcgac ccggtgctcc cgaaggcgaa cgtctgctac atgctccggg tgcagcgaga
     1321 gcggcaacgc cagcagttct tcccgagcgt ccgggagtac tcgaggaggt tcgggctgac
     1381 gcgcgaccgg gtcgcgatgc tgccggaggg cgcgttgatc atgcaccccg gcccgatgaa
     1441 ccgcggggtc gagatcgcgt ccgaggtggc ggacctcccg cagtcgctga tcaccgacca
     1501 ggtcacgaac gggatcgcgg tccgcatggc gctgctcttc ctcctcctcg gcggggaggc
     1561 ggcctgatgg caccggtgct gctgcgtggt ggac
//
LOCUS       DASZOB010000192         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_105303, whole genome shotgun sequence.
ACCESSION   DASZOB010000192 DASZOB010000000
VERSION     DASZOB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105303"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VG709_03185"
     CDS             complement(<1..1088)
                     /locus_tag="VG709_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWC32111.1"
                     /translation="MASTKTKYVYGFREGNRDMKDLLGGKGANLSEMTNLGLPVPPGF
                     TITTAACLEYLSGGDFPEGMWAEVQDHLRGLESEMGRRLGDRDNPLLVSVRSGAKYSM
                     PGMMDTVLNLGLNDRSVLGLARQARSDRFAFDSYRRFIQMFGRIVMGVPGEAFDEAFE
                     RAKAKKGKNAKDTDLGPSDLRDVVERFKRIVRDHAGRLFPDDPKEQLRMAVEAVFGSW
                     NNERAKTYRKQYRIPDDLGTAVNVVAMVFGNLGDDSGTGVAFTRDPSTGAQGVYGDYL
                     RNAQGEDVVAGIRNTLPLAQLRQLNRQAYEELLEIMRKLEKHYRDMCDIEFTIENRKL
                     WMLQTRVGKRTGGAAVKMAVDMANERLI"
     gene            1209..1535
                     /locus_tag="VG709_03190"
     CDS             1209..1535
                     /locus_tag="VG709_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32112.1"
                     /translation="MPVREKLRAITDDLGSQARVARALGVSPSRVSRWLRTEDPGPEN
                     RRKVEGLEFVLSYLLSFLHPDTAVDWLNGSDPFLNGFRPIDYLVRGRITEVIGAARAF
                     RAGSFA"
     gene            1537..2088
                     /locus_tag="VG709_03195"
     CDS             1537..2088
                     /locus_tag="VG709_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HWC32113.1"
                     /translation="MLYRRFPLVPGAALSEPGGPLFVPILDQGDGRHDNPDAYGALYL
                     SRSPVSPVAEMLVRLRNVALRPDHLLSEGFPYALAAFDEADVSLLDLDDPANLVAREL
                     RPSGVATRQRDETRPVALRLYEEGHAGFEWWSTIEASWINVTVFAERAASRLTLAGTP
                     EPLTLDHPVVREAADAVGVLVGG"
     gene            2141..>2515
                     /locus_tag="VG709_03200"
     CDS             2141..>2515
                     /locus_tag="VG709_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32114.1"
                     /translation="MKRTALAAIVLASVGLVTPATAEDLPAVTSDNVEYLGTLTEDAA
                     TGAVSAKIVGKYLYVAGWRSFSIYEISDPARPVHQSTTPLGFQLYHEDVDSNGKILVL
                     AENLQGPAARLHVWEVTNKQTPA"
BASE COUNT          353 a          899 c          849 g          414 t
ORIGIN      
        1 ctgatgagcc gctcgttcgc catgtcgacc gccatcttca cggcggcgcc accggtgcgc
       61 ttgccgacgc gcgtctggag catccagagc ttgcggttct cgatcgtgaa ctcgatgtcg
      121 cacatgtcgc ggtagtgctt ctcgagcttc cgcatgatct cgagcagctc ctcgtacgcc
      181 tggcggttga gctggcggag ctgcgcgagc gggagcgtgt tccggatgcc ggccacgacg
      241 tcctccccct gcgcgttccg gaggtagtcg ccgtagacgc cctgcgcgcc cgtcgagggg
      301 tcgcgcgtga acgcgacgcc ggtccccgag tcgtcgccga ggttgccgaa caccatcgcg
      361 acgacgttga ccgcggtccc gaggtcgtcc gggatgcggt actgcttccg gtacgtcttc
      421 gcgcgctcgt tgttccacga cccgaacacc gcctcgaccg ccatccgcag ctgctccttg
      481 gggtcgtccg ggaacagccg gccggcgtga tcgcgcacga tccgcttgaa gcgctctacg
      541 acgtcgcgta ggtcgctcgg cccgaggtcg gtgtccttcg cgttcttgcc cttcttcgcc
      601 ttggcgcgtt cgaaggcctc gtcgaacgcc tcgccgggca cccccatgac gattcggccg
      661 aacatctgga tgaagcggcg gtacgaatcg aacgcgaagc ggtcgctgcg cgcctgacga
      721 gcgaggccga ggaccgagcg gtcgttgagc ccgaggttga ggaccgtatc catcatcccg
      781 ggcatcgagt acttcgctcc cgagcggacc gagaccagaa gcgggttgtc gcggtcgccg
      841 aggcggcgcc ccatctcgct ctcgaggccc cggaggtggt cttggacctc ggcccacatc
      901 ccctccggga agtcgccgcc ggagaggtac tcgaggcacg cggccgtcgt gatcgtgaag
      961 ccgggcggaa cggggagccc caggttcgtc atctccgaga ggttggcccc cttcccgcca
     1021 aggaggtcct tcatgtccct gttgccctct cggaagccgt agacgtactt cgtcttggtg
     1081 ctggccatcg gtcctccctg cgatccgctc ccagcactgt accgccgccc gtgaggtcgc
     1141 gctccgagcg caacggattc cgttgcggta cgatgggtcg tgtcgcaggg gggtcggacg
     1201 atatgccggt gccggtcagg gagaagctac gagcgatcac cgacgacctg ggcagccaag
     1261 cccgcgtcgc gcgcgctctc ggcgtgagcc cgtcgcgtgt gagccggtgg ctcaggaccg
     1321 aggaccccgg ccccgagaac cgccggaagg tcgaggggct cgagttcgtg ctgtcgtacc
     1381 tgctgtcgtt cctccacccg gacacggccg tcgactggct gaacggctcg gacccgttcc
     1441 tgaacgggtt ccggccgatc gactacctcg tccggggccg gatcaccgag gtgatcggcg
     1501 ccgcccgcgc cttccgggcg gggtcgttcg cctgacgtgc tctaccggcg cttcccgctc
     1561 gtcccgggag cggcgctcag cgagcccggg ggcccgctgt tcgtgccgat cctcgaccag
     1621 ggtgacggac ggcacgacaa cccggacgcg tacggcgcgc tctacctgag ccgctccccc
     1681 gtgtctcccg tcgccgagat gctggtgcgg ctgcggaacg tcgcgctgcg gcccgaccat
     1741 ctcctcagcg aggggttccc ctacgcgctg gctgcttttg acgaggccga cgtgtcgctg
     1801 ctcgacctcg acgacccggc caacctcgtc gcgcgggagc tgcgtccctc cggggtcgcg
     1861 acacgccaac gcgacgagac gcggccggtc gcgctccgcc tctacgagga gggtcacgcc
     1921 gggttcgagt ggtggtcgac gatcgaggcg agctggatca acgtgaccgt gttcgcggag
     1981 cgggccgcct cgcgcctcac gctcgccggc acgccggagc cgctcaccct ggatcacccg
     2041 gtggtgcgcg aggccgccga cgcggtcggc gtgctcgtcg gcgggtgact cggtcacgcg
     2101 gtcccgtaca atctcgcaaa acgcccgaag gagactgccc gtgaagcgca ccgccctcgc
     2161 cgccatcgtc ctcgcctcag tggggctcgt gacccccgcc acggcggagg accttcccgc
     2221 ggtgacgtcc gacaacgtcg agtacctcgg cacgctcacc gaggacgccg cgaccggcgc
     2281 cgtgagcgcg aagatcgtcg ggaagtacct gtacgtggcc ggttggcgga gcttctcgat
     2341 ctacgagatc tccgaccccg cgcgaccggt ccaccagagc acgacgccac tcggcttcca
     2401 gctgtaccac gaggacgtcg acagcaacgg gaagatcctc gtgctcgcgg agaacctcca
     2461 ggggcccgct gcccgcctcc acgtctggga ggtgacgaac aagcagacgc cggcc
//
LOCUS       DASZOB010000193         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_24253, whole genome shotgun sequence.
ACCESSION   DASZOB010000193 DASZOB010000000
VERSION     DASZOB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24253"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..465)
                     /locus_tag="VG709_03205"
     CDS             complement(127..465)
                     /locus_tag="VG709_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWC32115.1"
                     /translation="MAPASIEDLERLDIRVGTVTRCEPFPEARKPAYKLWIDFGSGIG
                     LKASSARITDRYEPGDLPGRQVLGVVNFPPRRVGPFVSEVLTLGVYADRDVVVLVSPD
                     GPVPNGSRLG"
     gene            complement(465..1256)
                     /locus_tag="VG709_03210"
     CDS             complement(465..1256)
                     /locus_tag="VG709_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32116.1"
                     /translation="MAAANVIEVGESNFDEAVLTTSSDVPVVVDFWAGWCRPCLVLGP
                     VLERLADEYDGRFVLAKVDVDANPGLAARYGIQGIPAVKAFRDGRVASEFVGAQPEAV
                     VRSFLDGIVPSAADDLASSGARLEGSGDLDGAEAAYRKALEIEPDHPRAGVGLARLLA
                     ARGADADARSILARLPADDDARRLASLLSLRAGDGDGAPSGSAAAAAAAGDYRRALEE
                     ALRLISGGGDDRERARDLMIRVFDVLGDDHPLTREFRPRLAAALF"
     gene            complement(1310..1717)
                     /locus_tag="VG709_03215"
     CDS             complement(1310..1717)
                     /locus_tag="VG709_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWC32117.1"
                     /translation="MIAVGGAMLLTGVMSAPAEAITAKQRQLARLVNEFRVSNARSRL
                     SLTAPLSRAAQRNARRMAQIQDVTHSSDLPCGRNWGAETVGIGDSVTQAFRRMKNSGD
                     HRAHLLNRAAERVGTGVKRGGDDRFYVTVILCG"
BASE COUNT          290 a          670 c          654 g          251 t
ORIGIN      
        1 catgaggcac gaacacattc agaagcccgt cggcgccgga tgtctcacgg acgaagccga
       61 ccacgcggtc ggtgatgtcg acgacgatgg cgccgctcgt gtcgaccgtg aacaggtcgc
      121 tccgcatcag ccgagcctcg acccgttcgg gaccgggccg tcgggcgaga cgaggacgac
      181 gacgtcgcgg tccgcgtaca cgccgagcgt cagcacctcg gacacgaacg gcccgacgcg
      241 ccgcggcggg aagttcacga cgccgaggac ctgccggccc ggcagatcgc ccggctcgta
      301 ccggtcggtg atccgggcgc tcgacgcctt caggccgatc ccggacccga agtcgatcca
      361 gagcttgtac gccggcttcc gcgcctcggg gaacggctcg cagcgcgtca cggtcccgac
      421 cctgatgtcg aggcgctcga ggtcctcgat gctcgcggga gccatcagaa cagcgcggcc
      481 gcgagccgcg ggcggaactc gcgcgtcagc ggatggtcgt ctccgaggac gtcgaagacg
      541 cggatcatga ggtcgcgtgc tcgctcgcgg tcgtcgccgc caccggagat cagccggagc
      601 gcctcctcga gcgcgcggcg gtagtccccg gccgccgccg cggccgccgc ggatccggac
      661 ggcgcgccgt cgccgtcgcc ggcccggagg gacagcagcg acgcgaggcg gcgagcgtcg
      721 tcgtcggccg ggagccgggc gaggatggag cgagcgtccg cgtcggcgcc tcgcgccgcg
      781 agcaatcggg cgaggccgac gcccgcgcgg gggtggtcgg gctcgatctc gagcgccttc
      841 cggtaggcgg cctcggcgcc gtctaagtcg cctgagccct cgagcctagc gcccgacgag
      901 gcgaggtcgt cggcggcgga cgggacgatg ccgtcgagga acgaccgcac gacggcctcg
      961 ggctgcgcac ctacgaactc ggacgcgacg cggccgtccc ggaacgcctt cacggccggg
     1021 atcccctgga tgccgtaccg ggcagcgagg ccggggttcg cgtcgacgtc gaccttggcg
     1081 aggacgaagc gaccatcgta ctcgtcggcg aggcgctcga ggaccggtcc gagcacgaga
     1141 cacggcctgc accaaccggc ccagaaatcc actacgaccg ggacatcgct cgaggtagtg
     1201 aggacggcct cgtcgaagtt cgactcgccg acctcgatga cgttcgccgc ggccatcgct
     1261 acaagcgtag caacggcggc ggcgccgtca gccgtcccta tttaccccgc tagccgcaga
     1321 gaatgaccgt cacgtagaag cggtcgtccc caccgcgctt cactccagtc cccacgcgct
     1381 ctgcagcccg gttcaagaga tgtgcccggt ggtcccccga gttcttcata cgccggaacg
     1441 cctgcgtgac ggagtccccg atgccgaccg tctccgcacc ccagttacgg ccacagggga
     1501 gatcgctcga atgtgtgacg tcttggatct gagccatgcg cctcgcattt cgctgcgcgg
     1561 cacgcgacag gggtgccgtc agcgagagcc gggagcgggc gttgctcaca cggaactcgt
     1621 tcacgagccg cgcgagctga cgctgcttgg cggtgatcgc ctcggcgggg gccgacatca
     1681 cgccggtcag cagcatggcg cctcccacgg cgatcacggc cgcggaacgt cggatacggg
     1741 ctgcgcgtgt catggttccc ccttgtcccg tcggcgggca gacgctccgc ctgaactgct
     1801 tgcccatact cccacgggcg tcacgcgccg tctagagcga acgggccatc caatccgcgc
     1861 cgtgc
//
LOCUS       DASZOB010000194         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_124951, whole genome shotgun sequence.
ACCESSION   DASZOB010000194 DASZOB010000000
VERSION     DASZOB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124951"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VG709_03220"
     CDS             complement(<1..157)
                     /locus_tag="VG709_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWC32118.1"
                     /translation="MFFEHRTLYGLKGEVPDEIEPIELGKARIHREGDDVTVVATGRL
                     VHEALGAA"
     gene            complement(188..622)
                     /locus_tag="VG709_03225"
                     /pseudo
     CDS             complement(188..622)
                     /locus_tag="VG709_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
     gene            complement(661..1569)
                     /locus_tag="VG709_03230"
                     /pseudo
     CDS             complement(661..1569)
                     /locus_tag="VG709_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
BASE COUNT          224 a          603 c          528 g          289 t
ORIGIN      
        1 gcgcggcgcc gagcgcctcg tgcacgaggc ggccggtcgc gacgaccgtc acgtcgtccc
       61 cctcgcggtg gatgcgcgcc ttgccgagct cgatgggctc gatctcgtcc gggacctctc
      121 ccttgagacc gtagagcgtg cggtgctcga agaacatgat cgggttgtcg tcgtagatcg
      181 cagtccagag gaggccgcgg acgtcctcgg gcgtcgacgg aaagacgacc ttcaggcccg
      241 ggacgtgcac gaactagccc tcgagctgct gcgcgtgctg ggcgcctggc gaccagccgg
      301 cgcctccctg cgtgcggatc gtcagcggca ccttgatctg gccgcccgac atgtagcggt
      361 gcttggccgc ctggctcacg atctgctcca tcgcgagcgt cgtgaagtcc tggtacatga
      421 tctcgacgat cggccgcatc ccggcgatgg ccgccccgac gcccgcgccg acgatcgcca
      481 tctcggagat cggcgtgttc cgcacacgat cggggccgaa ctcgtcgagc atcccctgcg
      541 tgacgcccat cgagccgccc atctcggcga tgtcctcgcc catgatgaag acgtcctcgt
      601 cacggcgcat cgccgtcgcc atggcgtcgc ggacggcctc cctgtacgac agttccggca
      661 ctaggcgtac acgttcttca gcgcgtcctc gggcttcggg tcggtgccct gcttcgagaa
      721 ctcgacggcc tcgtccgcta gcctcgtcgc ctcggcgtcc atctcctccc actcatcgtc
      781 cgagagctcg accttctcgc gaagcctggt gagggggtcc tgggtctggc ggagctcccg
      841 cacctcctct ttcggccggt acacctgcgg gtcgcagacg tagtgacccg tcagccgatg
      901 cgtctttcag acgagcagga ccgggccgtc cccctcgcgc gcgtgcttca gcgccttctg
      961 ggtcgtcttg tacacggcct cgacgtcctg gccgtcgacc ttcatccccg tcatcccgta
     1021 cgcctcggcg cgcttgctca ggtcgtcgat cgggagctgc tgcttcgccg gcgtcgactc
     1081 cgcgtacccg ttgtcctcgc acacgaacag gacggggacg ttccagagct gagcgaggtt
     1141 cagggactcg tggaaggtgc cgatgttggt cgccccgtcg ccgaagaagg cgactgcgac
     1201 acgcggctcc ttgcgcatcc tgaacgcgag ggcggccccg acgatggccg ggaggccacc
     1261 gccgacgacc gcgttcgcgc ccaggttgcc gcgctcgacg tcgtacaggt gcatcgagcc
     1321 gccgtacccg cggctgcacc cctccagttt gccgtacagc tcggccatga cctcgttcgg
     1381 cggcgtcccc ttcgccagcg tgtggccgtg cgcgcggtgc gtcgaggcga tgacgtcccc
     1441 gtcctcgagg gcgcggcaga cgccgactgc gaccgcctcc tggccaatgg cgacgtggag
     1501 gaacccgggc agctcgcctg ctcggaagcg ctcctcgacc ttctcctcga agcggcggac
     1561 gagcagcatc tcccgcagga agtccttcag acgctcactc gccgaaacct gccccttttc
     1621 ctcgagcgtg gccacgccgg gaac
//
LOCUS       DASZOB010000195         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84758, whole genome shotgun sequence.
ACCESSION   DASZOB010000195 DASZOB010000000
VERSION     DASZOB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84758"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VG709_03235"
     CDS             <1..698
                     /locus_tag="VG709_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWC32119.1"
                     /translation="PEVACVIVEPVAANMGVVAPRAGFLETLREVTSAHGALLVFDEV
                     ITGFRVARGGAQERYGISPDLTVLGKVMGGGFPCAAFGGRADVMERLAPVGPVYQAGT
                     LSGNPVAVAAGAAALDLVERLDPYEALAATAERLCAGVSDALSAAGVPHTMNRVESLF
                     SVFFGERPVRSFDDARAADHAAYARFFGAMLERRIYLPPSGYEGWFLGTAHDDAAIER
                     TIEAARAAAAAAF"
     gene            complement(695..1180)
                     /locus_tag="VG709_03240"
     CDS             complement(695..1180)
                     /locus_tag="VG709_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32120.1"
                     /translation="MQRRSSEEIASAVVAAPVALALLFAAVLMMGVLVLVLSLTYGAR
                     AETAACPAPDEVAKDARRADVAPGAKLAPASAAPLVSATDAWGVACGEPGAREAARSP
                     ATYGLISGPAFGIEAPGKPVWVVAFDDACVPTEDGCRATDLAVVVDALSGERIASLPR
                     A"
     gene            complement(1303..>2080)
                     /locus_tag="VG709_03245"
     CDS             complement(1303..>2080)
                     /locus_tag="VG709_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32121.1"
                     /translation="ASRAARKRGRSLELAILAQAHGAAATSTLEGFLVVVAAIVLFVG
                     SVFLLLAAVFGIRMGYLMTATGFFGFWVLLAVLWTFGSWAFGAPGTLPNLGPRGELPS
                     WIPVAQGTDLRSETLPVVDQYPRGDWRSPEDAAATAEVDPATLAFQEFLAEQANEELE
                     REGIEGEVSPESFQVTNVRFADADDTRLAAATAFSATGGREVEVVGYLDEWNLEFPSW
                     LALGSGLVGFAVHVPFLDRAERKRKDVRTGGEQSPWRGPA"
BASE COUNT          298 a          787 c          730 g          265 t
ORIGIN      
        1 gccccgaggt ggcctgcgtg atcgtggaac ccgtggcggc caacatgggg gtcgtcgcgc
       61 cgagggcggg gttcctggag acgctccggg aggtcacctc cgcccacgga gcgctgctgg
      121 tgttcgacga ggtcatcacc ggtttccgcg tcgcccgcgg cggggcccag gagcgctacg
      181 ggatctcgcc ggacctcacg gtgctcggca aggtgatggg cggcgggttc ccgtgcgcgg
      241 cgttcggcgg tcgcgccgac gtgatggagc ggctcgcgcc ggtcggaccc gtgtaccagg
      301 ccgggacgct gtccgggaac cccgtcgcgg tcgccgccgg cgccgccgcg ctggatctcg
      361 tcgagcgcct cgacccctac gaggccctcg cggccaccgc ggagcgcctc tgtgcgggcg
      421 tctcggacgc gctgtctgcg gccggtgtcc cgcacacgat gaaccgcgtc gagtcgctgt
      481 tctcggtctt cttcggcgag cggccggtgc gcagcttcga cgacgcacgc gcggccgacc
      541 acgccgcata cgcgcggttc ttcggcgcga tgctcgagcg taggatctac ctgccgccgt
      601 ccgggtacga gggctggttc ctcggaacgg cgcacgacga cgcggcgatc gagcggacga
      661 tcgaggcggc gcgagccgcc gcggcggcgg ccttctaggc ccgcggaagc gacgcgatcc
      721 gctcgccgga gagcgcgtcg acgacgacgg cgaggtcggt cgcgcggcag ccgtcctccg
      781 tggggacgca cgcgtcgtcg aacgcgacga cccagaccgg cttcccggga gcctcgatcc
      841 cgaacgccgg cccggagatg agcccgtacg tcgccggcga ccgcgcagcc tcgcgcgcgc
      901 cgggctcgcc gcaagcgacc ccccatgcgt ccgtcgcgga cacgagtggc gccgcggatg
      961 ccggcgcgag ctttgcgccc ggcgccacgt cagcgcggcg ggcgtccttc gcgacctcgt
     1021 cgggggcggg gcacgcggcg gtttccgctc gggctccgta ggtgagcgag agcacgagca
     1081 cgagaacgcc catcatgaga acggcggcga acaggagcgc gagcgcgacc ggcgccgcga
     1141 cgaccgccga cgcgatctct tcggatgacc tccgctgcaa gtccgctagc gggcgatcgt
     1201 gaagagcatc gcgatgaaga tcgcgaccac catcaccgtg aaccagatga aggagaggcc
     1261 gccctcctcg atcgccgcga ccacgacgtc catcactacc ccctaggcag gtccacgcca
     1321 gggcgactgc tcgccgccgg tccgcacgtc cttacgcttg cgctcggccc ggtcgaggaa
     1381 cggcacgtgc accgcgaacc cgacgaggcc gctcccgagc gcgagccacg acgggaactc
     1441 gaggttccac tcgtcgaggt acccgaccac ctccacctcg cgccccccgg tcgcggagaa
     1501 ggccgtcgcc gccgcgagcc tcgtgtcgtc agcgtccgcg aaccggacgt tcgtgacctg
     1561 gaagctctcc ggcgacacct cgccctcgat cccctcgcgc tccagctcct cgttcgcctg
     1621 ctcggcgagg aactcctgga acgcgagcgt cgccgggtcg acctcggccg tcgcggccgc
     1681 gtcctccggc gaccgccagt ctcccctcgg gtactggtcg acgaccggga gcgtctcgga
     1741 ccggaggtcg gtcccctgcg ccaccgggat ccacgacggc agctcgccgc gcggaccgag
     1801 gttcgggagc gttcccggcg ccccgaacgc ccacgacccg aacgtccaca ggacggcgag
     1861 caggacccag aacccgaaga acccggtcgc cgtcatgagg tatcccatgc ggatcccgaa
     1921 caccgccgcg agcagcagga agacgctccc gacgaacagc acgatcgcgg cgacgacgac
     1981 gaggaacccc tcgagcgtgc tcgtggcggc cgcgccgtgc gcctgcgcga ggatcgcgag
     2041 ctccaagcta cgacccctct tcctcgccgc cctgctggcc
//
LOCUS       DASZOB010000196         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125011, whole genome shotgun sequence.
ACCESSION   DASZOB010000196 DASZOB010000000
VERSION     DASZOB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125011"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1786)
                     /gene="carB"
                     /locus_tag="VG709_03250"
     CDS             complement(<1..>1786)
                     /gene="carB"
                     /locus_tag="VG709_03250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWC32122.1"
                     /translation="RQVLVEEYLAGWDEFELEVMRDRKDNEVVVCSLENLDPMGVHTG
                     DSVTVAPQMPLPADAYQELRDAAAAIVRAVGVETGGSNIQFARDRDTGDVRVIEMNPR
                     VSRSSALASKATGYPIAKVAAKLAVGYSLDEIPNDLTGTTPASFEPTLDYVVVKFPCF
                     AFDKFPGADRTLGTQMKSVGEAMGIGRTFTEAFLKAMRSRELDGVTHSPWRRLEDVPD
                     GVHPWFRAQIDEVRDTLAELRSLDDLVAGDWLRLKRLGLADADVAAACGAEEATARAK
                     RRSCGVGPAYRRVDSCAGEVEAPSNYLYSTWGEADESRRVHSGKRTVAVLGSGPNRIG
                     QGIEFDYCCVHAARSFRALGFEAVMVNCNPETVSTDYDVSDRLYFEPLDAEAVLEVLR
                     RERPAGVAIQLGGQTPLRIAGEIEAEGFRILGTPSDAVDVAEDRGRFAALCDRLGLAV
                     PAWGAATGPAEASAVAERIGYPVLVRPSYVLGGRRMRVCYRREDVEAAVDTDASVLVD
                     VFLERALEIDVEALCDGADTYVAAIMQHVEEAGVHSGDSSCVLPAPSLDPATLAAIGD
                     AVRRLAPALGVIGLVNVQLALAEARLSVL"
BASE COUNT          258 a          648 c          636 g          244 t
ORIGIN      
        1 ccagcacgct cagccgcgct tcggcgagcg cgagctggac gttgacgagg ccgatgacgc
       61 cgagcgcggg cgcgaggcgg cggacggcgt cgccgatcgc cgcgagggtc gccggatcga
      121 gcgagggcgc gggcagaacg cacgacgaat cgcccgagtg gacacccgcc tcctcgacgt
      181 gctgcatgat cgcggccacg taggtgtcgg ctccgtcgca gagcgcctcg acgtcgatct
      241 cgagcgcacg ttcgaggaag acgtcgacga gcacggaggc gtcggtgtcc acggccgcct
      301 cgacgtcctc ccgtcggtag cagacgcgca tgcgccgccc cccgaggacg tacgagggcc
      361 gcacgaggac cgggtaaccg atccgctccg cgacggcgga ggcctccgcg ggcccggtcg
      421 cggcgcccca ggcgggaacc gcgaggccga ggcggtcgca gagcgcggcg aagcggccgc
      481 ggtcctccgc cacgtcgacc gcgtcggacg gcgtcccgag gatccggaaa ccctccgcct
      541 cgatctcgcc cgcgatcctg agcggcgtct ggcctccgag ctggatcgcg acgccggccg
      601 ggcgctcacg gcgcaggacc tcgaggacgg cctcggcgtc gagcggctcg aagtagaggc
      661 ggtcggagac gtcgtagtcc gtcgagacgg tctccggatt gcagttcacc attaccgcct
      721 cgaacccgag cgcgcggaag ctgcgggcgg cgtggacgca gcagtagtcg aactcgatcc
      781 cctggccgat ccggttcggt ccggatccga ggacggccac ggtccgcttt cccgagtgga
      841 cgcggcgaga ctcgtcggct tccccccacg tcgagtagag gtagttggag ggtgcttcca
      901 cctcgccggc gcaggagtcg acgcgccggt aggcgggccc gacgccgcac gagcgccgct
      961 tcgcccgagc cgtcgcctcc tcggcgccgc acgccgccgc gacgtcggca tccgccaggc
     1021 cgagccgctt caggcgaagc cagtcgccgg cgacgaggtc gtcgagcgag cgcagctccg
     1081 cgagcgtgtc ccgcacctcg tcgatctgcg cgcgaaacca cggatggacg ccgtcgggga
     1141 cgtcctcgag gcgccgccag ggcgaatgcg tgaccccgtc gagctcgcgg cttcgcatcg
     1201 ccttcaggaa cgcctcggtg aacgtccggc cgatgcccat cgcctctccg accgacttca
     1261 tctgcgtccc gagcgtccgg tccgcgcccg ggaacttgtc gaatgcgaaa caggggaact
     1321 tgaccacgac gtagtcgagc gtcggctcga agctcgcagg cgtcgttccg gtcaggtcgt
     1381 tcggtatctc gtcgagcgag tagcccaccg cgagcttcgc ggcgaccttg gcgatcgggt
     1441 acccggtcgc cttgctcgcg agcgcggagc tccgcgagac gcgcgggttc atctcgatca
     1501 cccgcacgtc gcccgtgtcg cggtcgcggg cgaactggat gttcgacccg cccgtctcga
     1561 cgccgaccgc ccggacgatc gcggcggccg cgtcccggag ctcctggtag gcgtcggcgg
     1621 gaagcggcat ctgcggggcg acggtgaccg agtcgcccgt gtgcacgccc atcgggtcga
     1681 ggttctcgag cgagcacacg acgacctcgt tgtccttccg gtcgcgcatc acctcgagct
     1741 cgaactcgtc ccagcccgcg aggtactcct cgacgaggac ctgccg
//
LOCUS       DASZOB010000197         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_84870, whole genome shotgun sequence.
ACCESSION   DASZOB010000197 DASZOB010000000
VERSION     DASZOB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84870"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2185)
                     /locus_tag="VG709_03255"
     CDS             complement(<1..>2185)
                     /locus_tag="VG709_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HWC32123.1"
                     /translation="RAGTLIVAPEGMVVVAIVGGRVTFAASSLVGTVAPAPAPTLSLE
                     DAVVAAASDGGGKVAPGDVSRRGRVRDWIRLNVRGLQGVQLARRGARPWPGAGVRSAY
                     ETYLMDEDGSRAFRHFVDARTGEVVIRWREVDFAGEPTWEVFPAYPPLDYSSTDTRET
                     WCWTPAEGCDLVLSTTLSRPPAARVPWDVNPRINSPTFTSFGNNARTTEKWLVPVGSA
                     TVAGVSTGPTVGVVPNTPRPNRDYVYPWTNRWFEQRCNPATAYLPPQLNDLDAAIANL
                     FAMHNRMHDWSYWLGFTEETWNGQDSNFGNTGPDRRENDPERGNAQAGALTGGPPAFL
                     SRDNANQFTPPDGSQPVTNMFLWQPVARAWYGPCVDGDFDMSVIAHEYTHMISNRMVA
                     GPDQRLVGLQANAMGESWSDLVAVEYLNEYGFVPVAGENPFAVGAYVTRDPQAGIRNY
                     GMNQSPLNYSDVGYDLTGPQVHADGEIWSATNFDIRQAMIERYGSLAARDATLQRRCA
                     DGQLPADRCPGNRRWVQLVFDAWLLMPPSGEVTMLDARDAMLAADRMRFGGANQDLLW
                     NVFARRGFGENATSKFGSNDDEPRPSFESPFSGEATLTFRPVDAAGNPIADAELYVGH
                     YEARVTPVADTAAHTDRPATVQMVPGTYDFVARADGYGMKRCTVTVAKTSDLGVAMPL
                     NLASRSNGAAAAGDGVNQDRLIDDTEETNWASFGADVRGRQVTVRL"
BASE COUNT          304 a          779 c          760 g          342 t
ORIGIN      
        1 gagccggacc gttacctgtc ggcccctcac gtcggcgccg aacgacgccc agttcgtctc
       61 ttccgtgtcg tcgatgagtc tgtcctggtt gacgccgtcc cccgcagccg cagcaccgtt
      121 cgaccgcgac gcgaggttga gcggcatcgc cacgccgagg tccgaggtct tcgcaaccgt
      181 cacggtgcag cgcttcatcc cgtatccgtc cgcgcgagcg acgaagtcgt acgtgccggg
      241 gaccatctgc acggtcgcgg gccggtccgt gtgtgcggcg gtgtccgcga ccggcgtcac
      301 gcgggcctcg tagtggccga cgtagagctc ggcgtccgcg atcgggttgc cggcggcgtc
      361 gaccggccgg aacgtcaggg tggcctcacc cgagaacggc gactcgaagc tggggcgcgg
      421 ctcgtcgtcg ttcgacccga acttgctcgt ggcgttctcg ccgaaccctc gccgcgcgaa
      481 gacgttccag agcaggtcct ggttcgcgcc gccgaaccgc atccgatccg cggcgagcat
      541 ggcgtccctc gcgtcgagca tcgtcacctc gccggagggc ggcatcaaga gccacgcgtc
      601 gaacacgagc tggacccacc gccggttccc cgggcatcgg tccgccggga gctgaccgtc
      661 ggcgcaccgg cgctgaagcg tcgcgtcgcg ggcggcgagc gagccgtagc gctcgatcat
      721 ggcttgacgg atgtcgaagt tcgtcgcgct ccagatctcg ccgtcggcgt gcacctgcgg
      781 ccccgtgagg tcgtagccga cgtcgctgta gttcagcggg ctctggttca tcccgtagtt
      841 gcggatcccc gcctgcgggt cacgggtgac gtacgcaccg acggcgaagg ggttctcccc
      901 cgcgacgggc acgaacccgt actcgttgag gtactcgacc gcgacgaggt ccgaccagct
      961 ctcccccatc gcgttcgcct gaagcccgac gaggcgctgg tccgggccgg caaccatgcg
     1021 gttggagatc atgtgcgtgt actcgtgcgc gatcacgctc atgtcgaagt cgccgtcgac
     1081 gcacgggccg taccacgccc gcgcgaccgg ctgccacagg aacatgttcg tcacgggctg
     1141 ggagccgtcg ggcggcgtga actggttcgc gttgtcgcgc gacaggaacg ccggcgggcc
     1201 gccggtgagc gcgccggcct gggcgttccc acgctcgggg tcgttctctc gacgatcggg
     1261 gccggtgttc ccgaagttcg agtcctggcc gttccacgtt tcctcggtga acccgagcca
     1321 gtacgaccag tcgtgcatcc ggttgtgcat cgcgaacagg ttcgcgatcg cggcgtcgag
     1381 gtcgttgagc tgcggcggga gatacgccgt ggcgggatta cagcgctgct cgaaccagcg
     1441 gttcgtccac ggatagacgt agtcgcggtt cggacgcggg gtgtttggga ccactcccac
     1501 ggtcggtccc gtgctgacgc ctgcgaccgt cgcggacccg acggggacga gccacttctc
     1561 ggtcgtccgg gcgttgttgc cgaagctcgt gaacgtcggc gagttgatcc gtggattcac
     1621 gtcccacggg acccgcgccg ccggcggccg cgacagcgtc gtcgagagca cgaggtcgca
     1681 tccttccgca ggcgtccagc accacgtctc ccgcgtatcc gtcgacgagt agtcgagcgg
     1741 cgggtacgcg ggaaacacct cccacgtcgg ctctccggcg aagtcgacct cgcgccagcg
     1801 gatcacgacc tcgccggtgc gcgcgtccac gaagtgacgg aacgctcggc tcccgtcctc
     1861 gtccatcagg tacgtctcgt acgctgaacg gacgcccgct ccgggccacg gccgcgcgcc
     1921 gcggcgcgcg agctggacgc cctggagccc ccgcacgttg agccggatcc agtcgcggac
     1981 ccgtccacgg cgagagacgt ccccgggtgc gaccttcccg cccccgtcgc tcgccgcggc
     2041 gacgacggcg tcctcgagcg agagcgtggg agccggggcg ggcgccacgg tcccgacgag
     2101 cgacgacgcg gcgaacgtca ccctgccgcc gacgatcgcg acgacgacca tcccctcggg
     2161 ggcgacgatc agcgtgccgg cacgc
//
LOCUS       DASZOB010000198         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_65056, whole genome shotgun sequence.
ACCESSION   DASZOB010000198 DASZOB010000000
VERSION     DASZOB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65056"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VG709_03260"
     CDS             complement(<1..741)
                     /locus_tag="VG709_03260"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWC32124.1"
                     /translation="MTIRSPSKLERLLHDGTFVVTGEVVPPRGADAGAVREQARSLLG
                     YADAVNVTDNPTSTAHMSPVAGSGLVAAEGLEPIVQLTCRDRNRLALTGDLLGAWALG
                     ARGVLCLTGDPIGGGDDAAARDVFDLSVLELVRFAVELRESGRLLSGAPASEAPRYFV
                     GAADVPLAHDYDAARLEAKADAGADFVQTQIVYDVDALGAWADAIRARGIFERMFVLV
                     GVAPPRSARTVAFMREHLPGVVVPDDVVR"
     gene            complement(738..>1614)
                     /locus_tag="VG709_03265"
     CDS             complement(738..>1614)
                     /locus_tag="VG709_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWC32125.1"
                     /translation="GTARVIDGKAMAAEIRGEVAERVRRLADVGIVPGLAAVLVGDDE
                     PSRIYVGAKQKAAAEVGIHSDRHDLAGDVTEAELLELILRLGDDRSVHGILVQLPLPD
                     HIPVLAVHEAIAPMKDVDALTPASVGRLVRGEATFLPATPFGIVEMLARTGIETRGAE
                     VVVVGRGPLVGLPLSIMLAQKSPHGNATVTLCHTATRDLPAHTRRADILVVAAGRPRT
                     VTADMVKPGAAVIDVAVNRTDDGLVGDVAFDEVSDVAGWITPVPGGVGPLTVAMLLVN
                     TVRAAEVAAGASASP"
BASE COUNT          221 a          600 c          582 g          211 t
ORIGIN      
        1 gcggacgacg tcgtccggta ccacgacgcc gggaaggtgc tcgcgcatga acgcgaccgt
       61 gcgagcgctc ctcggcggcg cgacgccgac cagcacgaac atccgctcga agatcccgcg
      121 tgcgcggatc gcgtcggccc aggcgccgag cgcgtcgacg tcgtagacga tctgcgtctg
      181 gacgaagtcc gcacccgcgt cggccttggc ctccaggcgt gcggcgtcgt agtcgtgcgc
      241 gagcggcacg tctgccgcgc ctacgaagta ccgcggcgcc tcggatgccg gggcgcccga
      301 gagcagccgg ccgctctccc gcagctcgac cgcgaaccgc acgagctcga gcaccgagag
      361 gtcgaacacg tcccgcgccg ccgcgtcgtc gccgccccct atcgggtcgc cggtgaggca
      421 cagcacgccg cgcgccccga gcgcccaggc tccgaggagg tctccggtga gcgcgagccg
      481 gttccgatcg cggcacgtga gctggacgat cggctcgagt ccctcggcgg cgaccagccc
      541 ggagcccgcg accggcgaca tgtgcgcggt cgacgtcggg ttgtcggtga cgttgaccgc
      601 gtcggcgtag ccgagcagcg acctcgcctg ctcgcgcacc gccccggcgt cggcgccgcg
      661 cggggggacg acctcacccg tcaccacgaa cgtgccgtcg tgcaggaggc gctcgagctt
      721 gctcggcgag cggatcgtca cggcgaagcg gaggcccccg cggcgacctc ggcagcgcga
      781 accgtgttga cgagcagcat cgcgacggtg agcgggccga cgccgcccgg caccggcgtg
      841 atccatcccg ccacgtcgga cacctcgtcg aacgcgacgt ccccgacgag gccatcgtcc
      901 gtgcggttca ccgcgacgtc gatgactgcg gcgcccggct tcaccatgtc ggcggtcacg
      961 gtccgggggc ggcccgcggc gacgacgagg atgtcggcgc ggcgcgtgtg cgccggaagg
     1021 tcgcgcgtcg cggtgtggca gagggtcacg gtcgcgttcc cgtgcggcga tttctgggcg
     1081 agcatgatcg acagaggaag ccctacgagt ggtccacggc cgacgacgac gacctcggcg
     1141 cccctcgtct cgatccccgt gcgcgcgagc atctcgacga tcccgaacgg cgtcgcgggg
     1201 aggaacgtcg cctcgccgcg gacgagtcgc cccacgctcg ccggcgtcag tgcgtcgacg
     1261 tccttcatcg gcgcgatcgc ctcgtgtacg gcgagcacgg ggatgtgatc cggaagcggc
     1321 agctgcacga ggatgccgtg gacggagcgg tcgtcgccga ggcgcaggat cagctccaag
     1381 agctccgcct cggtcacgtc gcccgcgagg tcgtggcggt cggagtggat cccgacctcc
     1441 gctgcggcct tctgcttggc gccgacgtag atccgcgacg gttcgtcgtc tccgacgagc
     1501 accgcggcga ggcccggcac gatccctacg tccgccagac gcctgacccg ctcggccacc
     1561 tcgccgcgga tctccgcggc catggccttg ccgtcgatca cccgggccgt cccg
//
LOCUS       DASZOB010000199         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_12271, whole genome shotgun sequence.
ACCESSION   DASZOB010000199 DASZOB010000000
VERSION     DASZOB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12271"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VG709_03270"
     CDS             <1..1470
                     /locus_tag="VG709_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWC32126.1"
                     /translation="AWRSGVFGTEAAGTDRGESPFAAGDGGVPATPSSPTPTVSPTPG
                     PINTEFEGLTTFRGNATRTYYGKGPVPTNPKVLWRFPESGGLCHESTDASGTRVWCGT
                     GWTGQPNVVEVEDGPAQVRFGAYSGAVHVLNARTGRELYEPFPTGDIIKGTVTSDPDG
                     FPLLYTGSRDNYFRILALDRGDRPRELWSLHANSAPNRVWNDDWDGSALVIDDYLLEG
                     GENSWFYVVKLNRGYDDRGRVTVDPEIRMTVPGYDDRLFADVGDQEVSIENSVAFDAS
                     RAIVYFANSGGLVQGWDVSDVLQGGDQYRRVFRFWTGDDTDASIVIDDEGFLYVASEL
                     QRFNARSKRVGQLMKLDPSKPRDPLVWSVDASDTGPEGLGGMWATAGLWRDAVFAATN
                     SGEILAVSRETGKVRWKIELPPPTWTSPVIVDGVLIVGDCAGVLHAYELRDRPLAGRP
                     KQLWRVKLEGCIESTPAVWDGMVYVGSRGGAVYGIGDRR"
     gene            1501..>1836
                     /locus_tag="VG709_03275"
     CDS             1501..>1836
                     /locus_tag="VG709_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWC32127.1"
                     /translation="MRPIQAGERAPAIPGLQLEDGRGIVMFYKVTCPTCQMVAPVAQR
                     LFERFPGRFAAVAQDPPDRIQDFGRHFGSSFPSLSEPPPYDAANGYGVRSVPTLFVVD
                     GGSVVDVVES"
BASE COUNT          267 a          612 c          677 g          280 t
ORIGIN      
        1 gcctggcggt ccggggtctt cgggacggag gccgccggga cggatcgagg cgagagcccg
       61 tttgccgccg gcgacggtgg ggtgccagcg acgccgtcgt caccgacgcc gaccgtgtcg
      121 cccacgcccg gaccgatcaa caccgagttc gagggcctca cgacgttccg cgggaacgcc
      181 acgcggacgt actacggcaa agggccggtg ccgacgaacc cgaaggtcct gtggcggttc
      241 cccgagagcg gagggctttg ccatgagtcc acggacgctt cggggacgcg cgtgtggtgc
      301 gggaccggct ggaccggcca gccgaacgtc gtggaggtcg aggacggtcc cgcgcaggtc
      361 cgcttcggcg cgtacagcgg cgcggtccac gtcctgaacg cgcgcaccgg gcgagagctg
      421 tacgagccgt tcccgaccgg cgacatcatc aaggggaccg tcacgtcgga ccccgacggc
      481 ttcccgctcc tctacacggg gtcgcgcgac aactactttc ggatcctcgc gctcgaccgc
      541 ggcgaccgcc cgcgtgagct gtggagtctc catgcgaact cggcgccgaa ccgcgtgtgg
      601 aacgacgact gggacggctc cgcgctcgtc atcgacgact atctcctgga gggcggcgag
      661 aactcgtggt tctacgtcgt gaagctgaac cggggctacg acgaccgcgg tcgcgtcacg
      721 gtcgatccgg agatccggat gacggtgccg ggatacgacg atcggctatt cgccgacgtc
      781 ggcgaccagg aggtgtcgat cgagaactcc gtcgcgttcg atgcctcgcg ggccatcgtg
      841 tacttcgcga actccggcgg gctggtccag gggtgggacg tctcggacgt cctccagggc
      901 ggcgaccagt atcggcgcgt cttccggttc tggaccggcg acgacaccga cgcgtccatc
      961 gtgatcgacg acgaggggtt cctgtacgtc gcgtcggagc tccagcggtt caacgcgcgg
     1021 tcgaagcgcg tggggcagct gatgaagctc gatccctcca agccgcgcga tccgctcgtc
     1081 tggtccgtcg acgcgtccga caccggcccc gaggggctcg gcggcatgtg ggccacggcg
     1141 gggctctggc gcgacgccgt cttcgccgcg acgaactcgg gcgagatcct cgcggtgagc
     1201 cgggagaccg gcaaggttcg ctggaagatc gagctgccac cgccgacgtg gacgtcgccg
     1261 gtcatcgtcg acggcgtcct catcgtcggg gactgcgcgg gcgtcctcca tgcgtacgag
     1321 ctccgcgatc gaccgctcgc ggggagaccg aagcagctgt ggcgcgtgaa gctggagggc
     1381 tgcatcgagt cgacgccggc cgtctgggac gggatggtct acgtcggatc gcgcggcggc
     1441 gccgtgtacg ggatcggcga cagaaggtag cccgccgcac ggcatgggta ccgtagccgc
     1501 gtgaggccga tccaggcggg ggagcgagcg ccggccatcc ccgggctgca gctcgaggac
     1561 gggcgcggga tcgtgatgtt ctacaaggtc acgtgcccga cgtgtcagat ggtcgcgccg
     1621 gtcgcccagc ggctcttcga gcggttcccc ggcaggttcg cggcggtggc gcaggatccg
     1681 ccggatcgga tccaggactt cggccgccat ttcgggtcct cgttcccatc gctctccgag
     1741 cccccgccgt acgacgcagc gaacgggtac ggcgtccggt cggtcccgac gctcttcgtg
     1801 gtcgacggcg gcagcgtcgt cgacgtcgtc gagtcg
//
LOCUS       DASZOB010000200         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_65173, whole genome shotgun sequence.
ACCESSION   DASZOB010000200 DASZOB010000000
VERSION     DASZOB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65173"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VG709_03280"
     CDS             <1..794
                     /locus_tag="VG709_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HWC32128.1"
                     /translation="AEEIFFVDLSSPNGATIADGRGDGTIVDDDDPTPSVSIGDVTVT
                     EGNSGTTSAVFTVSLSGASGTDVTIPYSTADGTATAGSDYTAASGTLTIPAGQTSRTL
                     AIAVTGDTVDETDEPFFVNMGSPTGATVADGQGQGTITDDDEPPPSTAPELSIGDASV
                     LEGPPGTKTALRLTVTLSSPQPTNVTFSYASVDHTATAASGDYVAKSGTGTIVAGATT
                     TTLRVDVRGDAIDEAEEIFFVDLSSPNGATIADGRGEGTIVDDDP"
     gene            894..1439
                     /locus_tag="VG709_03285"
     CDS             894..1439
                     /locus_tag="VG709_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32129.1"
                     /translation="MPDWLPWVIAAAVVIIVVVVAFFVAQRRRSTALRDRFGPEYDRT
                     MATTDGRREGERELRERQERRKELEIRPLAPDAQRRYVEQWRLVQERFVDDPTGAIAE
                     ADRLVTQVMRDRGYPMDDFEQRAADVSVDHPNVAEDYRRAHEVAVRRDDAETDTEDLR
                     RAMIHYRALFTDLLETSETEA"
     gene            complement(1443..2141)
                     /gene="npdG"
                     /locus_tag="VG709_03290"
     CDS             complement(1443..2141)
                     /gene="npdG"
                     /locus_tag="VG709_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWC32130.1"
                     /translation="MTGRVAVVGGTGDLGFGLVLRLAAAGVRVTIGSRARERADAAAG
                     RAREVLGGDAPVQGAENAGAFAEAEVAFVTVPYAGQAEIYRSIKDHVGGDLIVCDTTT
                     PLATAVGGRPWQVLRPWHGSAAEQARALLPRGTRLVAGFHSVGAEQLETLEHAADGDV
                     LLCGDDDDAKGTVGALVDRVPDLRWVDCGRLEMARVLEPLTAVLISVNRRYGIHDAGI
                     RVTGRDAWGAPRPD"
BASE COUNT          347 a          820 c          766 g          285 t
ORIGIN      
        1 aggccgaaga gatcttcttc gtcgacctct cgagcccgaa cggagccacg atcgccgacg
       61 gccgcggcga cggcacgatc gtcgacgacg acgaccccac gccttccgtc tcgatcgggg
      121 acgtgacggt caccgagggc aacagcggca cgacgtcggc ggtcttcacg gtgtcgctgt
      181 ccggagcgag cggcacggac gtgacaatcc cgtactcgac cgcggacggc accgcgaccg
      241 cggggtccga ctacacggcc gcgagcggca cgctcacgat cccggccgga cagacgtcgc
      301 ggaccctcgc gatcgccgtg accggcgaca cagtcgacga gaccgacgag cccttcttcg
      361 tgaacatggg atctccgacg ggagcgaccg tcgccgacgg gcagggacag ggaacgatca
      421 ccgacgacga cgagccgccg ccgtccacgg cgcccgagct ctcgattggc gacgcctcgg
      481 tcctcgaggg gccacccggg accaagaccg ccttgcgcct caccgtgacg ctgtcctctc
      541 cccagcccac gaacgtcacg ttctcgtacg cgtcggtcga ccacacggcc accgccgcct
      601 ccggtgacta cgtcgccaag agcggaaccg gcacgatcgt cgcgggagcg acgaccacga
      661 cgctccgcgt cgacgtgagg ggcgacgcga tcgacgaggc cgaagagatc ttcttcgtcg
      721 acctctcgag cccgaacggc gccacgatcg ccgacggccg cggcgagggc acgatcgtcg
      781 acgacgaccc gtagcagccg tttccgtccc ctcacggggg gaataccgag agtgactggc
      841 tggcgccgcg cacgcggctg gccgttcgcc agcacgacgg gcaaggaggg accatgccgg
      901 attggcttcc ctgggtgatc gcggccgccg tcgtcatcat cgtcgtggtc gtggcgttct
      961 tcgtggcgca acgccgccga tcgaccgcgc tacgcgaccg gttcgggcct gagtacgacc
     1021 ggacgatggc gaccaccgac ggcaggcggg agggagagcg ggagctccgc gagcgacagg
     1081 agcgccggaa ggagctcgag atccgaccgc tcgcccccga tgcgcagcgg cgatacgtgg
     1141 agcagtggcg gctcgtgcag gagcggttcg tcgacgaccc gaccggcgcg atcgcggagg
     1201 ccgatcgcct cgtcacgcag gtcatgcgcg accgcgggta tccgatggac gacttcgagc
     1261 agcgcgccgc ggacgtctcg gtcgatcatc cgaacgtggc cgaggactac cgtcgcgcgc
     1321 acgaggtcgc cgtgcgccgg gacgacgccg agaccgacac cgaggacctg cggcgcgcga
     1381 tgatccacta ccgcgcgctg ttcacggatc ttctcgagac gagcgagacc gaggcctgag
     1441 ggtcagtccg ggcggggcgc tccccacgcg tcccgcccgg tcacccggat cccggcgtcg
     1501 tggatcccgt agcgccggtt caccgagatc aggaccgcgg tcagcggctc cagcacgcgc
     1561 gccatctcga gccgtccgca gtcgacccat cggaggtcgg ggacccggtc gacgagcgca
     1621 ccgacggtcc ccttcgcatc gtcgtcgtcg ccgcacagca ggacgtcgcc gtccgccgca
     1681 tgctcgaggg tctcgagctg ctccgcgccg accgagtgga accctgcgac gagccgtgtc
     1741 cctcggggca gcagcgccct cgcttgctcg gcggccgagc cgtgccacgg ccgcaagacc
     1801 tgccaggggc gtccgccgac cgcagtggcc agcggcgtgg tcgtgtcgca cacgatgagg
     1861 tcgccgccga cgtggtcctt gatcgagcgg tagatctcgg cctgcccggc gtacgggacc
     1921 gtcacgaacg cgacctccgc ttcggcgaat gctccagcgt tctccgcgcc ttggaccggc
     1981 gcgtcgcctc cgaggacctc gcgcgcccgt ccggcggcgg cgtctgctcg ctcccgcgcc
     2041 ctcgacccga tcgtcacgcg aacgcctgcg gccgcgagcc ggagcaccag gccgaagccg
     2101 agatcgccgg ttccgccgac gacggcgacg cgccctgtca ccgcgtcggg tggtattcgc
     2161 cgaagacgcg acgcagccgg tccgagacct ccccgagcgt cgcgcgtgcc ttcagggc
//
LOCUS       DASZOB010000201         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173802, whole genome shotgun sequence.
ACCESSION   DASZOB010000201 DASZOB010000000
VERSION     DASZOB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173802"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2390
                     /locus_tag="VG709_03295"
     CDS             <1..2390
                     /locus_tag="VG709_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32131.1"
                     /translation="IARIVPELRRMYGDIPAPFELPPEQERRYLFNSIRDFVARAADL
                     RPLLLEHLAEALDDIPALVLGTYRDVELEVDRPLARSLESLLRRRLAERMSLKRLDEA
                     GVAASIAALAGQVPPPEIVRVVFAETEGNAFFVEEVFEHLHEEGRLLDDRGEWRREVR
                     VGEVDVPESVRLVIGRRLQRLSDEARKLLAAAATIGRVFDVNLLDAVTGGGEDELLDA
                     LEAAEAARLVSPLADDPARVEFSHELIRQTLLATLSTLRRQRLHRKVAEGIEARLGDR
                     SEDRAEELVHHLLNAGGGADPERLLRYLGAAGERGMAAAAWEDALRHFENALTLVEGD
                     RRARADLLVKLGFALRSVARMQDAAAAWREALDVYSEEGDSEAVAELCADLAFDLGYS
                     GRTVEGAEFVQRGLVASEGIRNAARVRLLASGVAYLAWSGDRPASVEMEREALALARE
                     LGDPTLEAGVQGARATADYLYMDLRGTVEAARRSLELTDPTARPWERTQVLSLLEFAH
                     LLRGESTQRDEVGEELVPLAERIGSGFALLLHHRAESFRDVGRTGDLRTFERYAREDL
                     ELCARLEQMWVAQSHTFLAMAAFWQGRWDEAIEHDRRGVEQELPGAFDGWSAAVLLLH
                     LAESGQQEEAVDLWRGWKERVPSVEGDIPFGAWAMALFAVLSLSILGRREEVATLAPY
                     AERALEEGTVLSVFGGVPVSMMVALSTAAAERWEEAESRFAEAMRVCEELPHRINVPE
                     VRRFHAMALLQWGDASERDRARAMLERAREEYRSLGMPRHAEVTERLLSAPLHPA"
     gene            complement(2378..>3349)
                     /locus_tag="VG709_03300"
     CDS             complement(2378..>3349)
                     /locus_tag="VG709_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32132.1"
                     /translation="TPRAASWSRTTRSRMRSRRRRTDVVSSLYASRRDADTSPLRRIL
                     EIITEQLERLEHDVTRSYEEWFVETDDEWAVPYLGDLVEVAIGDAARGDTEVALERFA
                     RAGVARFEDDVLSIAQDWWPHGHAEEALNLRWAVGAVAAVRDERFVEAAYRALRGRWH
                     AFRGARVTRETFERAIRASEPALRTLEPVTIADFERDAHMPALLDVFRAVEAVKPTNA
                     KWVATSKTLYFLLPDLVPPIDNRFTIPFLRRSGIRVPPELEPGTLRAAFTVFSSLARL
                     AGRERLERLSRRYGTPIGMARVVDFAIAARITSRSGGREPRPATHAG"
BASE COUNT          439 a         1156 c         1280 g          474 t
ORIGIN      
        1 agatcgcgcg gatcgtcccg gagctacgcc ggatgtacgg cgatatcccg gcgccgttcg
       61 agctcccgcc cgagcaggag cggcgctacc tgttcaactc gatccgcgat ttcgttgccc
      121 gcgcggcgga tctccggccg ctgctgctcg agcatctcgc cgaggccctc gacgacatcc
      181 cggcgctcgt gctcgggacg taccgcgacg tcgagctcga ggtcgatcgc ccgctggccc
      241 ggtcgctcga gagcctcctg cgtcgccggc tcgcggagcg catgtcgctg aagcggctcg
      301 acgaggcggg cgtcgccgcg agcatcgcgg cgctcgcggg acaggttccg ccgcccgaga
      361 tcgtgcgggt cgtgttcgcg gagaccgagg gcaacgcgtt cttcgtcgag gaggtgttcg
      421 agcacctgca cgaggaggga cggctcctcg acgaccgcgg cgagtggcgg cgcgaggtcc
      481 gggtcggcga ggtcgacgtc ccggagagcg tgcgcctggt gatcggccgg cggctccagc
      541 gtctgtcgga tgaggcgcgg aagctcctgg cggccgcggc gacgatcggg cgcgtcttcg
      601 acgtgaacct cctcgacgcg gtcaccggtg gcggcgagga cgagctgctc gacgcgctcg
      661 aggcggcgga ggcggcgcgc ctcgtctccc cgctggccga cgaccccgcc cgggtcgagt
      721 tctcgcacga gctgatccgg cagaccctgc tcgcgacgct gtccacgctg cggcgccagc
      781 gcctgcaccg caaggtcgcc gaagggatcg aggcgcggct gggcgaccgc tcggaggacc
      841 gcgctgagga gctcgtccac catctcctca acgcgggcgg cggcgccgat ccagagcggc
      901 tgctccggta cctcggcgcg gccggcgaac gcgggatggc cgccgcggcg tgggaggacg
      961 cgctccgcca cttcgagaac gcgctgacgc tcgtcgaggg cgaccgccgc gcccgcgccg
     1021 acctcctcgt gaagctaggc ttcgcgctcc gtagcgtcgc gcggatgcag gacgcggccg
     1081 ccgcgtggcg cgaggcgctc gacgtctact cggaggaggg cgactccgag gccgtcgccg
     1141 agctgtgtgc ggacctcgcg ttcgacctcg gctacagcgg ccggaccgtc gagggcgccg
     1201 agttcgtgca gcggggcctc gtcgcgtcgg aggggatccg caacgccgcg cgcgtccggc
     1261 tcctcgcgag tggcgtcgcg tacctcgcgt ggtcgggaga ccgaccggcc agcgtcgaga
     1321 tggagcgtga ggccctcgcg ctcgcccgtg agctcggcga tccgaccctc gaggcgggcg
     1381 tgcagggcgc ccgcgccacg gcggactacc tctacatgga cctccgcggc acggtcgagg
     1441 ccgcgcgccg ctctctcgag ctcacggatc cgacggcgcg tccgtgggag cgcacgcagg
     1501 tcctgtcgct gctggagttc gcgcacctgc tgcgtggtga gtccacccaa agggacgagg
     1561 tcggggagga gctcgtcccg ctcgccgaac gcatcggctc gggcttcgcg ctgctgctcc
     1621 accaccgcgc tgagtcgttc cgcgacgtgg ggcggaccgg cgatctccgg acgttcgagc
     1681 gctacgcgcg ggaggacctc gagctgtgcg cgcggctcga gcagatgtgg gtggcgcagt
     1741 cgcacacgtt cctcgcgatg gcggcgttct ggcaaggccg ctgggacgag gcgatcgagc
     1801 acgaccggcg cggggtcgag caggagctcc ccggggcgtt cgacgggtgg tcggccgcgg
     1861 tgctgctgtt gcacctcgcg gagtcggggc aacaggagga ggcggtcgac ctctggcgcg
     1921 gatggaaaga gcgagtgccc tcggtcgagg gcgacatccc gttcggggcg tgggcgatgg
     1981 cgctgttcgc ggtcctgtcg ctgtcgatcc tcgggcggcg cgaggaagtc gcgacgctcg
     2041 ccccgtacgc cgagcgggcg ctcgaggaag ggacggtgct gagcgtgttc ggcggcgtgc
     2101 ccgtgtcgat gatggtggcg ctatcgacgg ccgcggcgga gcgctgggaa gaggcggagt
     2161 cgcggttcgc cgaggcgatg cgcgtgtgcg aggagctgcc ccaccggatc aacgtgccgg
     2221 aggtgcgtcg gttccacgcg atggcgctgc tccagtgggg cgacgcgtcc gagcgcgacc
     2281 gcgctcgcgc gatgctcgag cgtgcgcgcg aggagtaccg gtcgctcgga atgccgcgcc
     2341 acgccgaggt gaccgagcgg ctgctctcgg cgccgcttca tccggcgtga gtcgcggggc
     2401 gcggttctcg gccgcctgaa cggctagtga tccgcgcggc gatcgcgaag tcgacgacgc
     2461 gcgccatccc gatcggcgtg ccgtagcgac ggctgaggcg ttcgagccgc tctcggcccg
     2521 cgagccgagc gagcgaggag aacaccgtga acgcggcgcg cagcgtcccg ggctcgagct
     2581 ctggaggcac gcggatcccg gagcgccgta ggaacgggat cgtgaagcgg ttgtcgatgg
     2641 gcggcacgag gtccgggagc aggaagtaca gcgtcttcga tgtggcgacc catttcgcgt
     2701 tggtcggctt gacggcctcg accgcgcgga agacgtcgag gagcgccggc atgtgcgcgt
     2761 cccgctcgaa gtcggcgatc gtgacgggct cgagcgtccg gagcgcgggc tccgaagcgc
     2821 ggatcgctcg ctcgaacgtt tcgcgcgtga cgcgcgcgcc gcggaacgca tgccatcgcc
     2881 cgcgaagcgc ccggtacgcc gcctcgacga accgctcgtc gcgaacggcg gcgacggcac
     2941 cgacggccca ccgcaggttg agcgcctcct ccgcgtgccc gtggggccac cagtcctgcg
     3001 cgatcgacag gacgtcgtcc tcgaaccgcg cgacgcccgc gcgagcgaac cgctcgagcg
     3061 cgacctcggt atcgccgcgg gccgcatccc cgatcgcgac ctcgaccaga tcgccgaggt
     3121 acgggacggc ccactcgtcg tccgtctcga cgaaccattc ctcgtagctc cgcgtgacat
     3181 cgtgctcgag ccgctcgagc tgctcggtga tgatctcgag gatccggcgg agcggcgacg
     3241 tatccgcgtc gcgacggctc gcgtagagcg acgagacgac gtcagtccgt cggcgcctcg
     3301 agcgcatacg cgatcgcgtc gttcggctcc agctcgcggc cctcggcgt
//
LOCUS       DASZOB010000202         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173815, whole genome shotgun sequence.
ACCESSION   DASZOB010000202 DASZOB010000000
VERSION     DASZOB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173815"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VG709_03305"
     CDS             complement(<1..924)
                     /locus_tag="VG709_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32133.1"
                     /translation="MKRPDTIAGGGAAVVCRDVTRSFGTVRALDSFTAEFPSEAVTGL
                     VGPNGAGKTTLLLILAGLLAPDGGSVRVGGLDPTRDARAVHRIVGWMPDFFGVYDDLT
                     PLEYLELFAAAYGVPEGARRASHVLSVVELEAVATARVHTLSRGQKQALGFARALLHR
                     PRILLLDEPAAGLDPHARIWLRAVIREQAAAGVAVVVSSHVLTELEEMIDVVVFVERG
                     ESRGAFSRTALPGATKEVVWRLRALDADALRRSLRAARAKARWVDGDRALVTLRDERA
                     AARLISRIVGAGVPLVEAAREERGLEQAYLAL"
     gene            1005..1517
                     /gene="greA"
                     /locus_tag="VG709_03310"
     CDS             1005..1517
                     /gene="greA"
                     /locus_tag="VG709_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWC32134.1"
                     /translation="MEGTRFRRNPGDEPSAVLTRDAYDRLRAELERLKGEGREHIAER
                     LLHARELGDIRENAEFDAAKNEQGLMEARIRELERMLKDPDIVEHASAAEIVESGTLV
                     TVRAVEDETDEDTYLVAASKEERAPGVRTVSVESPLGRALLGRRVGERVTYDAPGGRF
                     EYEIVGLDSR"
     gene            complement(1514..1708)
                     /locus_tag="VG709_03315"
     CDS             complement(1514..1708)
                     /locus_tag="VG709_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32135.1"
                     /translation="MRTFRLGEVFDVWGGPLDERCLGPYCEGDGATLEAAVDGRSIGG
                     DPRNLELRDGQEIVVEYASP"
     gene            1901..>2159
                     /locus_tag="VG709_03320"
     CDS             1901..>2159
                     /locus_tag="VG709_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32136.1"
                     /translation="MSLVIGGTLAYLFLPFPWWVVVVAVFVGVEIVEARIVLWAFRER
                     PRSGTEALVGHRGVLIGSDRVRIQGTSSRAPADGGADGAEVV"
BASE COUNT          309 a          770 c          796 g          284 t
ORIGIN      
        1 caacgcgagg tacgcctgct cgagtccccg ctcttcccgc gccgcctcga cgagcggcac
       61 gccggctccg acgatccgcg agatgaggcg cgccgcggcc cgctcgtcgc ggagcgtgac
      121 cagcgcgcgg tcgccgtcga cccacctcgc tttcgcgcgc gcggcgcgga gcgaccggcg
      181 cagcgcgtcg gcgtcgagcg ctcggagccg ccagacgacc tccttcgtcg ctccgggtag
      241 cgcggtccgg gagaaggcgc ctcgcgactc ccctcgctcg acgaagacaa cgacgtcgat
      301 catctcctcg agctcggtga ggacgtgcga cgagacgacg accgcgacgc ccgcggcggc
      361 ctgctcgcgg atcaccgcac gcagccagat gcgcgcgtgc ggatcgaggc cggccgccgg
      421 ctcgtcgagc agcaggatcc gcggacggtg gagcaacgcc cgggcgaacc cgagcgcctg
      481 tttctggccg cgcgagagcg tgtggacccg ggccgtcgcg acggcttcga gctcgacgac
      541 cgacaggacg tgcgaggcac ggcgcgcccc ctcggggacc ccgtacgcgg ccgcgaagag
      601 ctccaggtac tcgagcggcg tgaggtcgtc gtagacgccg aagaagtccg gcatccaccc
      661 gacgatgcgg tggacggcgc gggcgtcccg ggtcggatcg aggccgccga cgcgaacgct
      721 tccgccgtcg ggagcgagca accccgcgag gatcaggagg agggtcgtct ttcccgcgcc
      781 gttcggcccg accagcccag tgacagcctc gctcgggaac tcggcggtga acgagtcgag
      841 cgcgcggacg gtcccgaacg atcgcgtgac gtcgcgacac acgacggcgg ctccaccgcc
      901 ggcgatcgtg tccgggcgct tcaccgcggc gagcatatcc cggcgagcga gggcgccggc
      961 gtgcggggcg gcgacaggtg tggggtcgtt gctacattgg ccgcatggaa ggaacacgat
     1021 tcaggcgcaa ccccggcgac gagccgagcg cggtgctcac gcgcgacgcg tacgaccgcc
     1081 tccgcgctga gctcgagcgg ctgaagggcg aggggcgcga gcacatcgcc gagcggctgc
     1141 tccacgctcg cgagctcggc gacatccgtg agaacgcgga gttcgacgcc gcgaagaacg
     1201 agcagggcct catggaagct cgcatccgcg agctcgagcg gatgctgaag gacccggaca
     1261 tcgtcgagca cgcgtcggcg gccgagatcg tcgagagcgg aacgctcgtc acggtccgcg
     1321 ccgtcgagga cgagacggac gaggacacct acctcgtggc ggcgtcgaag gaggagcgcg
     1381 ctcccggcgt tcggaccgtc tcggtcgagt cgccgctcgg gcgggcgctc ctcggacggc
     1441 gcgtcggcga gcgcgtgacc tacgacgcgc ccggcgggcg gttcgagtac gagatcgtgg
     1501 gcctcgactc tcgctagggc gaggcgtact cgacgacgat ctcctggccg tctcggagct
     1561 cgaggtttcg agggtctccg ccgatcgatc ggccatcgac cgcggcctcg agcgtcgcgc
     1621 cgtccccctc gcagtagggg ccgaggcagc gctcgtcgag cgggccgccc cacacgtcga
     1681 acacctcccc gagacggaac gtccgcacct cctccgactc gacgtggatc gtgcccgact
     1741 agtcgtgcgt gtggatcggc gcgacgaagt tctgctccca gtgcgacccg cgggagaatc
     1801 gcgcgccgcc gatccggcgg ccgtgccgca ccggcatccg gctcgagctc catggtcccg
     1861 agcgtacccg tccccgcccg gcgcgattta ggctcgtcgc gtgtcgctcg tgatcggagg
     1921 gacgctcgcg tacctcttcc tgccgttccc gtggtgggtc gtcgtcgtgg cggtcttcgt
     1981 cggcgtcgag atcgttgaag cgcggatcgt gttgtgggcg ttccgcgaac gcccccggtc
     2041 ggggaccgag gcgctggtcg gccaccgggg cgtcctgatc ggctcggacc gggtccgcat
     2101 ccagggaacc tcgtcccgcg cgcccgccga cgggggcgcc gacggagccg aggtcgtcg
//
LOCUS       DASZOB010000203         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_165872, whole genome shotgun sequence.
ACCESSION   DASZOB010000203 DASZOB010000000
VERSION     DASZOB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165872"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /gene="tmk"
                     /locus_tag="VG709_03325"
     CDS             <1..1295
                     /gene="tmk"
                     /locus_tag="VG709_03325"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWC32137.1"
                     /translation="GVSFLKDHAFARAMTIGIVLAFTGAGSVMSLGPIFVDEALGAKP
                     SAWGVLVTSVGIGLGIGMFAVGQLSKLIEKDTLFSTTILTGAVAIALLAAMPNIALAA
                     LVTVAIGAAAGATWVNGYTLLQENVTDEMRGRVFGSLTALARLGLFVSLAGFPVLAQG
                     LGTHTIGLGDRSVTLAGTRLAMWVGTIVILAGGFLARRGLAGSRLTRPRPLKLFPRLR
                     KIERHGSFIVFEGVEGAGKGTQIRLAKDFLEGKGLDVLVTREPGGTGLGERLRASLLE
                     TGDEGIDYRAEALMFAASRAQHVSRVIRPALEEGKVVLCDRYLDSSLAYQGTGRGLGE
                     QDVLSLNVWATQGLFPDLVILLHIEPDKGLARAARIPDRIEAEDEKFHMKVADAFVRI
                     AEDHPERFVVVPADDPPDVVQKRVQDALEKFLKTEEDT"
     gene            1292..2437
                     /locus_tag="VG709_03330"
     CDS             1292..2437
                     /locus_tag="VG709_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HWC32138.1"
                     /translation="MIPVLRRVPGQDEAIQFLERAAKAPRHAYLFAGPEGSGKQLAAR
                     AFTAALLCARGGCGECRDCRLALEDRHPNVVLVEPEGRDIRVGTGPDDDGTARWMAAR
                     AALTAPEPGRKVFRIDQADRLTEEAADVLLKVLEEAPAETVFVLSSARPDEIPETVRS
                     RCQAVAFRPLPERFVVETLEREGVEPDRARLAARLAGGNLGRARRIATDPKGLAFRDA
                     ALEALEMAERGSGGALDAADRLIASAAGYRKRLATRLRGELDPFTDPDTGLPLEQYRA
                     SVRRLKERHARQERRAEREFLDWSLLALEAWFRDAMLAAAGGDRSWAINVDRAEDAAA
                     HDADVAARAVAALEAARAALADETNLNARLVLEDAFLELTAALRPAA"
     gene            2477..2550
                     /locus_tag="VG709_03335"
     tRNA            2477..2550
                     /locus_tag="VG709_03335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Thr,seq:cgt)
     gene            complement(2569..>3661)
                     /locus_tag="VG709_03340"
     CDS             complement(2569..>3661)
                     /locus_tag="VG709_03340"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HWC32139.1"
                     /translation="GGAETIRTTLATIAASMVTFLGVVFSITMVVLQLASNRFSPRVL
                     RTFLKDRVSQVTLGAFVATFTFSMVVLRTVPASGPQDLPVVSVIVAFTLVVASVVTFV
                     AYVNHMARAIRISRIAAAIGDETADVIAEQYPEREGDGGRGDEPRLGPTPSRTIPSPS
                     GGNLEAMNEGRLAALAREADVVLALKPRMGEFVPRGAPLVHVYGDGRLDDDRVLAEID
                     LGEERTLAQDPSFGFRQLVDIAERSLSPGTIDPTSAVQALDQLHDLLRDLATRELPER
                     RVTRDEDGEPRVIVPSMTWDEYLDLAVDEIVLYGRGSLQVAARLRAVLEDLDTVTSGD
                     RLRAVRDKISSLPPPPDPYAVSRDALEQM"
BASE COUNT          537 a         1273 c         1320 g          531 t
ORIGIN      
        1 aaggagtcag cttcctcaag gaccacgcgt tcgcgcgcgc gatgacgatc gggatcgtcc
       61 tggcgttcac cggcgcggga tcggtcatgt cgctgggccc gatcttcgtc gacgaggcgc
      121 tgggtgcgaa gccgagcgcg tggggagtcc tggtgacgtc ggtcggaatc gggctgggca
      181 tcgggatgtt tgcggtcggg cagctctcga agctcatcga gaaggacacg ctgttctcca
      241 cgacgatcct tacgggcgcg gtcgcgatcg cgctcctcgc ggcgatgccg aacattgcgc
      301 tcgctgccct ggtaaccgtc gcgatcggcg ccgccgccgg tgcgacgtgg gtgaacggat
      361 acaccttgct gcaggagaac gtcaccgatg agatgcgcgg tcgcgtcttc ggctcgttga
      421 cggcgctcgc gcgcctcggg ctgttcgttt cgctcgccgg cttcccggtc ctcgcacagg
      481 gcctcggcac gcataccatc gggctcggtg accgctccgt gacgctcgcg ggaacgcgac
      541 tggcgatgtg ggtcggcacg atcgtgatcc tcgcgggcgg gttcctggcg cggcggggcc
      601 tcgccggcag ccggcttaca cggccgcggc cgctcaagct gttcccgcgg ctccggaaga
      661 tcgagcgcca cggctccttc atcgtgttcg aaggcgtgga aggggccggc aaggggaccc
      721 agatccggct cgcgaaggac ttcctcgaag ggaagggcct cgacgtgctc gtcacgcgcg
      781 agcccggcgg caccggcctc ggcgagcggc ttcgcgcgtc gctgctcgag accggcgacg
      841 agggcatcga ctaccgggcc gaggcgctca tgttcgccgc gagccgcgca cagcacgtct
      901 cgcgggtgat ccggcccgcg ctcgaggagg ggaaggtcgt actctgcgac cggtatctcg
      961 actcctcgct cgcgtaccag gggaccggcc gcggcctcgg cgagcaagac gttctctcgc
     1021 tcaacgtctg ggcgacgcag gggctcttcc ccgacctcgt catcctgctc cacatcgagc
     1081 ccgacaaggg actcgcgcgc gccgctcgca tccccgaccg catcgaggcc gaggacgaga
     1141 agttccacat gaaggtcgcg gatgcgttcg tccggatcgc ggaggatcac cccgagcggt
     1201 tcgtcgtcgt tcccgccgac gaccctcccg acgtcgtgca gaagcgggtc caggacgcgc
     1261 tcgagaagtt cctgaagacc gaggaggaca cgtgatcccg gtcctgcggc gcgttcccgg
     1321 ccaggacgag gcgatccagt tcctcgagcg cgcggcgaag gcgccgcgac acgcgtacct
     1381 gttcgcgggg cccgagggat cgggcaagca gctcgcggcg agggcgttca cggcggcgct
     1441 cctgtgcgcg cgcgggggct gcggggagtg ccgtgactgc cggctcgcgc tcgaggaccg
     1501 gcacccgaac gtcgtcctcg tcgagccgga gggcagggac atccgcgtgg ggaccggacc
     1561 ggacgacgac ggcaccgcgc gatggatggc cgctcgggcg gcgctcacgg cgccggagcc
     1621 cggacggaag gtgttccgca tcgaccaggc cgaccgtttg accgaggagg cggcagacgt
     1681 gctgctcaag gtcctcgagg aggcgccggc cgagacggtc ttcgtgctgt cgtcggcacg
     1741 tcccgacgag atccccgaga ccgtccggtc gcgctgtcag gccgtcgcgt tccgcccgct
     1801 ccccgagcgg ttcgtcgtcg agacgctcga acgcgagggc gtcgagcccg accgcgcacg
     1861 cctcgcggcg aggctcgccg gcgggaacct cgggcgcgcg cgtcgcatcg cgacggaccc
     1921 gaagggtctc gccttccgtg acgccgcgct cgaggcgctc gagatggcgg agcgtgggag
     1981 cggcggagcg ctcgacgccg ccgaccggct gatcgcgtcg gcggccggct accggaagcg
     2041 gctcgcgacc cggctccgcg gggagctcga ccccttcacg gatcccgaca cggggctccc
     2101 gctcgagcag taccgcgcct cggtccgacg cctgaaggag cggcacgcgc gacaggaacg
     2161 ccgcgccgag cgcgagttcc tcgactggtc gctgctcgcg ctcgaggcgt ggttccggga
     2221 cgcgatgctc gcggctgcgg ggggcgatcg gtcgtgggcg atcaacgtcg accgcgcgga
     2281 ggacgcggcg gcccacgacg cggacgtcgc cgctagggcg gtcgcagcgc tcgaggcggc
     2341 gcgcgcggcg ctcgccgacg agacgaacct caacgcacgg ctcgtcctcg aggacgcgtt
     2401 cctcgagctc acggccgcgc ttcgacccgc cgcatagcgg tagcggccgg agcgccgttg
     2461 ctacaatcga cgacccgccg gagtagctca gttcggcaga gcacacgatt cgtaatcgtg
     2521 gggtcgtggg ttcgaatccc acctccggct cagtcctcgg cttgcgagct acatctgctc
     2581 gagcgcgtcg cgtgataccg cgtacgggtc cggcggcgga gggagcgaag agatcttgtc
     2641 acggaccgct ctgagccgat ctccggacgt cacggtgtcg agatcctcga gcaccgcgcg
     2701 gagcctcgcc gccacctgaa gggaacctcg cccgtagagg acgatctcgt cgaccgcgag
     2761 atcgaggtac tcgtcccacg tcatcgaagg gacgatcacc cgcggttccc cgtcctcatc
     2821 cctcgtgacg cgccgctcgg gcagctctcg cgtcgcgagg tcgcgcagca ggtcgtggag
     2881 ctggtcgagc gcctgcacgg ccgacgtcgg gtcgatcgtc cctggggaga gcgagcgctc
     2941 ggcgatgtcg acgagctgcc gaaacccgaa cgagggatcc tgcgcgagcg tccgctcctc
     3001 ccccagatcg atctccgcga ggacgcggtc gtcgtcgagg cggccgtcac cgtagacgtg
     3061 gacgagcggg gccccgcgcg ggacgaactc gcccatcctc ggcttgaggg cgaggacgac
     3121 gtcggcctct cgagccagcg ccgcgagccg tccctcgttc atcgcctcga ggtttccgcc
     3181 gctcggcgaa gggatcgtgc gcgaaggcgt ggggcccagg cgcggttcgt ccccacgccc
     3241 tccgtccccc tcgcgctccg gatactgctc ggcgatgacg tccgccgtct cgtcgccgat
     3301 cgcggccgcg atccgcgaga tccggatcgc gcgggccatg tggttcacgt acgccacgaa
     3361 cgtcacgacg ctggcgacca cgagcgtgaa cgcgacgatg accgaaacga cagggaggtc
     3421 ctgcgggccg gaggccggga cggtgcgcag gacgaccatc gagaacgtga atgtggcgac
     3481 gaaggcgccg agcgtcacct ggctcacccg gtccttcagg aacgtcctca gcacgcgcgg
     3541 cgagaaccgg ttgctggcca gctggagcac gaccatcgtg atactgaaca cgacaccgag
     3601 gaacgtgacc atcgacgcgg cgatcgtcgc gagcgtcgtc cggatcgtct cggcgcctcc
     3661 c
//
LOCUS       DASZOB010000204         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_85138, whole genome shotgun sequence.
ACCESSION   DASZOB010000204 DASZOB010000000
VERSION     DASZOB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85138"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1405)
                     /locus_tag="VG709_03345"
     CDS             complement(170..1405)
                     /locus_tag="VG709_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC32140.1"
                     /translation="MLRTVPVPDAKQLDDYREPAGDEAVERLLDSANALKGTRVVHVS
                     STAFGGGVSELLHTQVGLLRNLGIDTDWQVIEGSDDFFTVTKLVHNACQGAPASWNAH
                     LERTYLDRVRENAERFDPHADFVIVHDPQPLALVSMLDGQRQTAKWVWRCHIDLSTPH
                     EDVWAFFAPHVDRYDAVVFTLEDYMRPGLNTRVAIIPPSIDPLSPKNSWLDHDAVWEI
                     LLAYGIDPQRPVFTQISRFDPWKDPLGVIDAYRIAKEEVPDLQLVLAGSMAHDDPEGW
                     EFLERTEEHRRGDPDIHILTNLQDVGPVAINAFQRGSTVVTQKSIREGFGLTAAEAMW
                     KDKAVIAGDAGGLRLQVRDGETGFLVRTVEECAEHTVTLVRNAELRERMGRASREHVR
                     RNFLTLREVTDYLDLLASL"
     gene            1466..2812
                     /locus_tag="VG709_03350"
     CDS             1466..2812
                     /locus_tag="VG709_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWC32141.1"
                     /translation="MADQDRVLTPLVTRVARWGIVAWATIGLLVLAFFAFRYVVYPIR
                     IVFPPLVIAMIAVYLLNPIVTGLERRGLGRVWAALIVYLLGGAIVGTALYFLVPLVAE
                     QATNLADALPRIVGQASEAFQDVVGRFGVEVGEAPVAPDQQTITAFFGRFVSIAYGIV
                     EIAIIFILGPILGFYLLVDLPKIRRGLRAMIPARRRAEVESVLRKIGEAIGGFFRGQF
                     LVALFVGIASSLVLLLVGLPFWAVVGLVAGLFNLIPLIGPFIGGAVAVVVAMTTSAPA
                     GGVFGLEPGWPLAVGSAIALTVVQQLDNHILSPQIVGRTVRLHPVTVMLGLLIGGALL
                     GLWGMLLAIPTIAAIKILILHAWDTRVRWPPTAEAAEGLAGHPVEGAGASPEPTSSST
                     EPATLSPPGSAPVAPPRAPQASGWRDAMRRIFARRPGDEAPDEPAPAPPAGSRAEG"
     gene            complement(2809..>3185)
                     /locus_tag="VG709_03355"
     CDS             complement(2809..>3185)
                     /locus_tag="VG709_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWC32142.1"
                     /translation="VAVFLAVAALVWLWRRPPARLRRLDLSALGVREPAIVQFSAGSC
                     SPCRAAAPKLRAAAAEAQVRYMQVDVGDDPEVARTYGIRTVPTIAVTGRGGRVIEVWT
                     AVPDGDAVTGTARRARGTVVRV"
BASE COUNT          422 a         1126 c         1112 g          525 t
ORIGIN      
        1 cggtcgccat ccgacatcgc ggcggcgacc cagagcccgc cggtctcctg cacgactgcg
       61 gtgagggctg tcacgagacc gcccgagccc cggcgcgcga cgagctctcc gtccttctcg
      121 acgaacgtga ccggaccgcg gttcgatgcg acgacgatcc gcttctcggt cacagcgacg
      181 cgagcagatc gaggtagtcc gtgacctccc gcagcgtcag gaagttccgg cggacgtgtt
      241 cgcgcgacgc ccggcccatc cgctctcgga gctccgcatt acgaaccagc gtcaccgtgt
      301 gctcggcgca ctcctcgacc gtccgcacga ggaacccggt ctctccgtcg cgcacctgga
      361 gcctgagacc gccggcgtcg cccgcgatca ccgccttgtc cttccacatc gcctcggcgg
      421 cggtaagccc gaacccctcg cggatcgact tctgcgtgac gaccgttgat ccacgctgga
      481 aggcgttgat cgcgaccggc ccgacgtcct ggaggttcgt gaggatgtgg atgtcgggat
      541 ccccgcgccg gtgctcctcc gtgcgctcga ggaactccca accctcgggg tcgtcgtgcg
      601 ccatggagcc cgccagcacg agctgtagat ccgggacctc ctccttcgcg atccggtagg
      661 cgtcgatcac gcccagcggg tccttccagg gatcgaaccg tgagatctgc gtgaacaccg
      721 gccgctgagg gtcgatcccg tacgcgagga ggatctccca cacggcgtcg tggtccaacc
      781 acgagttctt cggcgagaga gggtcgatcg acggcgggat gatggcgacg cgggtgttga
      841 gaccggggcg catgtagtcc tcgagagtga acacgaccgc gtcgtagcga tcgacgtgcg
      901 gcgcgaagaa cgcccacacg tcctcgtggg gcgtcgagag gtcgatgtgg catcgccaca
      961 cccacttggc ggtctgccgc tggccgtcga gcatggagac gagtgccagc ggctgcgggt
     1021 cgtggacgat gacgaagtcg gcgtgcggat cgaagcgctc ggcgttctcg cggacgcggt
     1081 cgaggtacgt gcgctcgagg tgcgcgttcc acgacgcggg cgcgccctgg cacgcgttgt
     1141 ggacgagctt cgtgacggtg aagaagtcgt cggagccctc gatcacctgc cagtcggtgt
     1201 cgatgccgag gttccggagg agcccgacct gcgtgtgcag cagctccgac acgccgccgc
     1261 cgaacgcggt gctgctcacg tggacgaccc tcgttccctt cagcgcgttc gccgagtcga
     1321 gcagcctctc gaccgcctcg tcgccggcgg gttccctgta gtcgtcgagc tgcttcgcgt
     1381 cggggacggg aacggttctc agcatgccct tcaagtctag gtccgatcag agggcccgtc
     1441 gcaggtccga acctataatc ggccgatggc cgaccaggat cgggtcctga ccccgctcgt
     1501 cacgcgcgtc gctcggtggg gcatcgtggc gtgggccacg atcggcctgc tggtcttggc
     1561 gttcttcgcg ttccggtacg tcgtgtaccc gatccgcatc gtcttcccac cgctcgtgat
     1621 cgcgatgatc gcggtgtacc tcctgaaccc gatcgtgacc ggcctcgagc ggcggggcct
     1681 cgggagggta tgggcggcgc tcatcgtgta cctcctcggc ggcgcgatcg tggggaccgc
     1741 gctctacttc ctggtaccgc tcgtcgccga gcaggccacg aatctcgctg acgcgcttcc
     1801 gcggatcgtc gggcaggcgt cggaggcgtt ccaggacgtg gtcggacggt tcggcgtcga
     1861 agtcggcgag gcgccggtcg cgcccgacca gcagacgatc accgcgttct tcgggcggtt
     1921 cgtgtcgatc gcctacggga tcgtggagat cgccatcatc ttcatcctcg gaccgatcct
     1981 gggcttctac ctgctggtcg acctgccgaa gatcaggcgc gggctccgcg cgatgatccc
     2041 ggcgcggcgg cgggccgagg tcgagtcggt gctccgcaag atcggcgagg cgatcggcgg
     2101 cttcttccgc gggcagttcc tcgtcgcgct gttcgtcggc atcgcgtcgt cgctggtcct
     2161 gctcctcgtc gggctgccct tctgggccgt cgtcgggctc gtcgcggggc tgttcaacct
     2221 gatcccgttg atcggtccct tcatcggggg agccgtcgcg gtcgtcgtcg cgatgacgac
     2281 gtccgccccg gccggcggcg tcttcgggct cgagcccggc tggccgctcg cggtcggctc
     2341 cgcgatcgcg ctgacggtcg tgcagcagct cgacaaccac atcctcagcc cccagatcgt
     2401 cgggcggacc gtgcgcctgc acccggtcac cgtgatgctc gggctgctga tcggcggagc
     2461 gttgctgggg ctgtggggga tgctgctggc catcccgacg atcgccgcga tcaagatcct
     2521 gatcctccat gcgtgggaca cccgcgtgag gtggccgccg accgccgagg cggccgaggg
     2581 gctcgccgga caccccgtcg agggcgccgg agcgtcgccc gagccgacga gctcctccac
     2641 ggagccggct acgctatcgc cgccgggttc tgcgccggtt gcgccgccgc gggcccccca
     2701 ggcgagcggt tggagggatg cgatgcggcg gatcttcgcg aggcgcccgg gcgacgaggc
     2761 gccggacgag ccggctccgg ctccgcccgc gggctctcgc gccgaaggct agacgcgaac
     2821 gaccgttcct ctggcgcgtc gcgccgtgcc cgtgaccgcg tcgccgtccg gcacggccgt
     2881 ccacacctcg atgacgcggc cgccccgacc ggtcaccgcg atcgtgggga ccgtcctgat
     2941 cccgtacgtc cgtgccacct cgggatcgtc gccgacgtcg acttgcatgt atcgcacctg
     3001 cgcctccgcc gctgccgctc ggagcttcgg tgcggccgcg cggcacggcg agcacgagcc
     3061 cgccgagaac tgcacgatcg ccggctcccg gacgccgagg gccgacaggt cgagccgccg
     3121 caggcgtgcg ggcggtcggc gccacagcca cacgagcgcc gcgacggcga ggaacacggc
     3181 caccg
//
LOCUS       DASZOB010000205         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_105780, whole genome shotgun sequence.
ACCESSION   DASZOB010000205 DASZOB010000000
VERSION     DASZOB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105780"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VG709_03360"
     CDS             complement(<1..202)
                     /locus_tag="VG709_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32143.1"
                     /translation="MQAPKRAALVSDLYDDPREAEAAPLRGILEVIADQLERLEHDVT
                     RSYEEWFVETDDEWAVPYLGDLV"
     gene            402..1736
                     /locus_tag="VG709_03365"
     CDS             402..1736
                     /locus_tag="VG709_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32144.1"
                     /translation="MRRVLASIAFALGLVAASPAFASHNADEHTPNMTLVANRPYQSS
                     ETSTTSNGTDLAFQGDLLVAGNLSPGGFRLFDISTPSNPTLISQFVCNGSQSDVSIWG
                     DLVFVSVDGPRSSPACDAPAASLTETTTGSAWEGIRIVDISDPAKPRQIDTVRTDCGS
                     HTHTLVPDKNQKTSRVYLYVLSYPLTPVMPNCNVATHRKISVVEVPLQKPGAARVVST
                     PDVSPAIGCHDVTVFPREDMAGAACITESQTWDISNRARPRVVSHIVNPAINIHHSTT
                     FSWDGTTLAIGDELGGAAVAPGCPTDAGHLPLGAIWFYRVNPETAVASPAPVGFFEIE
                     NQELSTLCTAHNFNTVPMTNDRDILTTAWYDGGTAVVEFTNPAAPVQLGRYTPKTAPG
                     PATPWSSYWFNRYIYANNYTSPRGLDVFRIEDPFVALTQKTWRLNPQTMIGG"
BASE COUNT          298 a          615 c          604 g          253 t
ORIGIN      
        1 cgaccagatc gccgaggtac gggacggccc actcgtcgtc cgtctcgacg aaccattcct
       61 cgtagctccg cgtgacatcg tgttcgagcc gctcgagctg gtccgcgatc acttcgagga
      121 tgccgcgtag cggcgccgcc tcggcttcgc gcggatcgtc gtagaggtcg gagacgagcg
      181 ccgctcgttt cggtgcctgc accgcgcgac gaacactagt cgtccgcgcc gcgccggcga
      241 gggatcgggg cgacggggcg gctggcggcc gtcggcgccg gcgggcgctc acgtacctcg
      301 tcgacaacga ggacgcgtct ggaaggaacc cgtgaaccgc cgtcgaaggg gtactaggac
      361 ggacaaaacg tccgtccttg gaagcacagg aggagctgcc cgtgagacgc gtcctagcct
      421 ccatcgcctt cgcgctcggc ctcgtcgcgg cgtcgccggc cttcgcgagc cacaacgccg
      481 acgagcacac gccgaacatg acgctggtcg cgaaccgccc gtatcagagc tccgagacca
      541 gcacgacgtc gaacgggacg gatctcgcgt tccaggggga tctcctggtc gcggggaacc
      601 tgagccccgg cgggttccgg ctgttcgaca tctcgacgcc gagcaacccc acgctgatca
      661 gccagttcgt ctgcaacggc tcgcaatcgg acgtctcgat ctggggcgat ctggtgttcg
      721 tctcggtcga cggtccgcgg agcagcccgg cgtgcgatgc gccggcggcc agcctgacgg
      781 agaccaccac cggctcggcc tgggaaggga tccgcatcgt cgacatctcc gacccggcga
      841 agccgagaca gatcgacacg gtgcggaccg actgcggatc gcacacccac acgctcgttc
      901 ctgacaagaa tcagaagacg agtcgcgtct acctgtacgt cctgtcgtac ccgctcacgc
      961 ccgtgatgcc gaactgcaac gtcgcgacgc accggaagat ctcggtggtc gaggtgccgc
     1021 tgcagaagcc cggcgccgcg cgcgtcgtct cgacgcccga cgtgtcgccg gcgatcggat
     1081 gccacgacgt gacggtcttc ccgcgggagg acatggccgg agccgcgtgc atcaccgagt
     1141 cgcagacgtg ggacatctcg aaccgcgccc ggccgcgcgt cgtctctcac atcgtgaacc
     1201 cggcgatcaa catccaccac tcgacgacgt tctcgtggga cggcacgacg ctcgcgatcg
     1261 gggacgagct cggaggcgcg gcggtcgcgc cggggtgccc gaccgacgcg ggacacctcc
     1321 cgctcggcgc gatctggttc taccgggtga acccggagac ggccgtcgcg agccccgccc
     1381 ccgtcggctt cttcgagatc gagaaccagg agctctcgac gctgtgcacc gcgcacaact
     1441 tcaacacggt gccgatgacg aacgaccggg acatcctgac gacggcgtgg tacgacggcg
     1501 gcacggcggt cgtcgagttc acgaaccccg cggcgccggt gcagctcggc cgctacacgc
     1561 cgaagaccgc gcccgggccc gcgacgccgt ggtcgtcgta ctggttcaac cggtacatct
     1621 acgcgaacaa ctacacgagc ccgcgagggc tggacgtgtt ccggatcgag gacccgttcg
     1681 tcgcgctgac gcagaagacc tggcgtctga acccgcagac gatgatcggc gggtagcggg
     1741 gtcctcgagg cgagcgaggg gggggggggg
//
LOCUS       DASZOB010000206         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_145641, whole genome shotgun sequence.
ACCESSION   DASZOB010000206 DASZOB010000000
VERSION     DASZOB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145641"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VG709_03370"
     CDS             complement(<1..289)
                     /locus_tag="VG709_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HWC32145.1"
                     /translation="MSVKVGINGFGRIGRNFFRAAKSKGADIEFVAVNDITDAATLAH
                     LLKYDSVLGTLTEDVKAGEGSINVDGDEFQVLAERDPANLPWKDLGAEVVVE"
     gene            complement(348..1271)
                     /gene="rapZ"
                     /locus_tag="VG709_03375"
     CDS             complement(348..1271)
                     /gene="rapZ"
                     /locus_tag="VG709_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWC32146.1"
                     /translation="MSAVRSRRGRLDQPRLFPEAPDFTVITGLSGAGRSEAAKCLEDR
                     GYFVVDNLPPALIPKMAELLSQGEGPRRIATVVDVRGGAFFGEVADALSSLRELRVPY
                     RILFLEASDDVLLNRFAETRRKHPLAGDRVEGIRREREMMAQLREQADLIIDTTRLTP
                     HELRERIRETFASARPEAGMQVAVVSFGFKHGAPRDADLVFDVRFLPNPYWIHHLRAL
                     GGNEEPVHRYVTGQKDYAEFLDRLRALFDVAVPGYVAEGKSYLTIGIGCTGGRHRSVV
                     VADEIGAYFRDRGYPVNVQHRDLHRASTQAP"
     gene            complement(1268..>2760)
                     /gene="uvrC"
                     /locus_tag="VG709_03380"
     CDS             complement(1268..>2760)
                     /gene="uvrC"
                     /locus_tag="VG709_03380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWC32147.1"
                     /translation="PYAHAYAIRDTLDSLTRVFPVRTCSNGFFDQRRRAGRPCLYYDI
                     GRCLGPCVPEKTGVTEDGYRETVERMASFLAGDHRDVLRALERDMREAAGAEEFEKAA
                     RFRDRLGAARKVIESHEMVLGRAEDLDVVGLDEDDLEAAFQVFFVRRGRVVGQRGWVV
                     DRVEDLETPGLVSSFLQQLYMERDEIPRTVIVPAWPLDREVLERWLTDRRGGRVRISV
                     PARGEKRRLMRIVTTNAKDAFVRHKLKRASDFAARSRALKDLGDALGLDTPPLRIEAY
                     DVSNLGPESKVGSMVVFEDGLPKRSDYRRFEIKGVPGQDDFASMEEMLRRRFARYLKE
                     RDEPVDKRRRFAYPPSLVVVDGGRGQLGVALRVVDELGLQIPAVGLQKRLEEVVLPGQ
                     PEPLRIPRGSEALFLLQHVRDEAHRFAIEYHRKKRDRRAMASPLDEIPGIGPSRKRAL
                     LRRFGSLARLSRASREEIAATPGVGPALAAEIEAALRGRREAARPA"
BASE COUNT          411 a          972 c          964 g          413 t
ORIGIN      
        1 actcgaccac gacctcggcg ccgaggtcct tccacggcag gttcgcgggg tcacgctccg
       61 cgaggacctg aaattcgtcg ccgtcgacgt tgatcgagcc ctcgcccgcc ttgacgtcct
      121 ccgtgagcgt gccgagcacg gagtcgtact tcagcagatg ggcgagcgtc gcggcatcgg
      181 tgatgtcgtt caccgcgacg aactcgatgt cggcgccctt gctcttcgct gcgcggaaga
      241 agttcctgcc gatgcgcccg aacccattga ttccgacctt gacgctcatg tcgcctcctt
      301 gcgcatgggg aaccatcgta aatcgcgaga cgcggaggcc gcgacggcta cggggcctgc
      361 gtcgaggcgc ggtggaggtc gcggtgctgg acgttcacgg ggtaccccct gtcgcggaag
      421 tacgcgccga tctcgtcggc gacgacgacc gagcgatggc gaccgcccgt gcacccgatc
      481 ccgatcgtga ggtaggactt cccttcggcg acgtagccgg gcaccgcgac atcgaagagc
      541 gcccggagcc ggtcgaggaa ctccgcgtag tccttctgac ccgtcacgta ccggtggacg
      601 ggttcctcgt tccccccaag cgcgcgcaag tggtggatcc agtacgggtt cggaaggaag
      661 cggacgtcga agacgaggtc cgcgtcacga ggcgcgccgt gcttgaaccc gaaggagacg
      721 acggcgacct gcatgccggc ctcgggccgc gcgctcgcga acgtctcgcg gatccgctcc
      781 cggagctcgt gtggggtgag cctcgtcgtg tcgatgatga ggtccgcttg ctcccggagc
      841 tgcgccatca tctcgcgctc gcggcggatc ccctcgacgc gatcgccggc cagcgggtgt
      901 ttcctacgcg tctcggcgaa gcggttgagc aggacgtcgt cggacgcctc gaggaacagg
      961 atgcggtacg gcacacgaag ctctctgagc gacgagaggg cgtcggccac ctcgccgaag
     1021 aaggcgccgc cgcgtacgtc gacgaccgtg gcgatgcgtc ttgggccctc gccctgcgag
     1081 agcagctcgg ccatcttcgg gatcagcgcc ggcgggaggt tgtcgacgac gaagtagccg
     1141 cggtcctcca ggcacttggc ggcctcggac cgcccggctc cggagaggcc ggtgatcacc
     1201 gtgaagtccg gcgcctcggg gaacaggcgc ggctggtcga ggcggccgcg ccgcgaacgg
     1261 acggcgctca cgccggcctc gccgcctcgc gtcgtccgcg cagggcggcc tcgatctcgg
     1321 cggcgagggc cgggccgacg cccggcgtgg cggcgatctc ctcgcggctc gcccgggaga
     1381 gccgcgccag ggatccgaag cgccggagca gcgccctctt cctcgacggc ccgatgccgg
     1441 ggatctcgtc gagcggcgac gccatcgcac gccggtcccg cttcttgcgg tggtactcga
     1501 tcgcgaaccg gtgcgcctcg tcgcggacgt gctgcaggag gaacagcgcc tccgatcccc
     1561 tcgggatacg gagcggttcc ggctggcccg gaaggacgac ctcctcgagt cgcttctgca
     1621 gcccgacggc cgggatctgg agccccagct catcgacgac ccgcagagcg acgccgagct
     1681 gaccgcgccc gccgtcgacg acgacgagcg acggcgggta ggcgaacctc cgccgcttgt
     1741 cgacgggctc gtcgcgctcc ttcaggtatc tggcgaagcg cctgcgcagc atctcctcca
     1801 tcgaggcgaa gtcgtcctgt ccggggacgc ccttgatctc gaagcggcga tagtcggagc
     1861 gcttcgggag cccatcctcg aagacgacca tcgacccgac cttcgactca gggccgaggt
     1921 tcgagacgtc gtacgcctcg atccgcagcg gcggcgtgtc gaggccgagc gcgtcgccga
     1981 ggtccttgag cgcccgcgag cgggccgcga agtccgatgc gcgcttgagc ttgtgccgga
     2041 cgaacgcgtc cttggcgttg gtcgtgacga tccgcatgag gcgtcgcttc tcgcctcgtg
     2101 ccggcacgga gatccgaacg cggccgccgc gacggtcggt gagccaccgc tcgagcacct
     2161 cgcgatcgag cggccaggcc ggcacgatca ccgtgcgagg gatctcgtct cgttccatgt
     2221 acagctgctg gaggaacgaa gacaccaggc ccggcgtctc gaggtcctcc acgcggtcca
     2281 cgacccatcc gcgctggccg acgacccggc cgcggcgcac gaagaagacc tggaacgcgg
     2341 cctccaggtc gtcctcgtcg agcccgacga cgtcgaggtc ctccgcgcgg cccagcacca
     2401 tctcgtggga ctcgatgacc ttccgggccg cgccgaggcg gtcgcggaac cgcgccgcct
     2461 tctcgaactc ctctgcaccc gccgcctcgc gcatgtcgcg ctccagcgct cgaagcacgt
     2521 cgcgatggtc gccggccagg aacgacgcca tccgctcgac ggtctcgcgg taaccgtcct
     2581 cggtgacgcc ggtcttttcc ggaacgcagg gtccgaggca ccggccgatg tcgtagtaga
     2641 ggcacgggcg ccccgcgcgg cgccgctggt cgaagaagcc gttcgagcac gtccgaaccg
     2701 ggaagacgcg agtcagcgag tcgagcgtgt cgcggatcgc gtacgcgtgc gcgtaggggc
//
LOCUS       DASZOB010000207         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_145648, whole genome shotgun sequence.
ACCESSION   DASZOB010000207 DASZOB010000000
VERSION     DASZOB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145648"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VG709_03385"
     CDS             complement(<1..184)
                     /locus_tag="VG709_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HWC32148.1"
                     /translation="MKTLYRASRVRTLSLLGQGDWVLVDGRHVERVGTGDPPAADEVV
                     DLPETTIVPGFIDAHVH"
     gene            complement(209..991)
                     /locus_tag="VG709_03390"
     CDS             complement(209..991)
                     /locus_tag="VG709_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HWC32149.1"
                     /translation="MTRRRHGTTRSGDSRGRCSAVELPGRDTSPAAGEGTLRHTAVRR
                     TDPVVVTLEPKPSGGHRRMATLSRRDAQRWAAIARRVEPAVRPLLGPEAVGPAGMGRA
                     GLRAALRRARGAAARLAARSPFVVRSDISDFYGSVMPGALLAALRTTARGSDAAAAAA
                     MLEGWGSDGYAGLPVGPPGSAVLAEAVLAQVDEELRPLPFLRWADDYLIAVPSERAGA
                     EVIERLDVALERIGLRRSLPKTAVGAGSEFRWIGTSFPPTNA"
     gene            complement(992..>1565)
                     /locus_tag="VG709_03395"
     CDS             complement(992..>1565)
                     /locus_tag="VG709_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWC32150.1"
                     /translation="TEDELREQTSAVPSLRALGEGTLTSRLWRRPWISVLGIDAPTVV
                     EASNTLVPAARAKVSLRVAPGDDPERAMDALVAHLEANAPWGAEVRVERGAAARPFAV
                     QPKGDVFEAASSAFTEAWGRDPLFVGQGGTIPFVADFADTYPNATILLTGVGDPDSRA
                     HGANESVDLDELFRGCVAETLFLEKLASRD"
BASE COUNT          224 a          581 c          563 g          197 t
ORIGIN      
        1 ggtgcacgtg cgcgtcgatg aagcccggga cgatcgtcgt ctccggaagg tcgacgacct
       61 cgtccgccgc gggtgggtcc cccgtcccca cgcgttcgac gtggcgtcca tcgacgagca
      121 cccagtcgcc ctgtcccagc agcgagagtg tccggacccg cgaggcgcgg tagagcgttt
      181 tcatccgcgc ggattctatg gtcggagctc aggcgttcgt tggagggaac gaggtcccga
      241 tccagcggaa ctccgacccg gcgccgacgg cggtcttggg caacgagcgg cggagcccga
      301 tccgctccag cgcgacgtcg agccgctcga tcacctccgc gccggcccgc tcggacggca
      361 ccgcgatgag gtagtcgtcg gcccagcgga ggaacggaag cggacgcagc tcctcgtcga
      421 cctgcgcgag caccgcctcg gcgagcacgg cggatcccgg cgggccgacg gggagccccg
      481 cgtacccgtc gctcccccat ccctcgagca tcgccgcagc ggctgcggcg tcggagccgc
      541 gcgccgtggt tcgaagcgcg gcgagcagcg cgcccggcat caccgaaccg tagaagtcgc
      601 tgatgtcgga gcggacgacg aacggagaac gagcggcgag acgtgccgcg gccccgcgcg
      661 cgcgccggag cgccgcacgg agacccgctc gacccatccc agcggggccg acggcttccg
      721 ggccgagcag cggtcgcacc gccggctcga cccgtcgcgc gatggccgcc caccgctgcg
      781 cgtcgcggcg cgacagcgtc gccatccggc gatgtcctcc cgacggcttc ggctcgagcg
      841 tgacgacgac cggatccgtg cgtcggacgg cggtgtgtct cagcgtgccc tccccggccg
      901 cgggggaggt atcccggccc gggagttcta ccgcggagca acggccacgc gaatcccccg
      961 agcgggtggt tccgtgccgt cgccgggtga ttcagtcgcg cgaggcgagc ttctccagga
     1021 acagcgtctc ggcgacgcac ccgcggaaca gctcgtcgag gtcgacgctc tcgttggcgc
     1081 cgtgggcgcg cgagtcggga tccccgacgc cggtaaggag gatcgtcgcg ttagggtacg
     1141 tgtcggcgaa gtccgcgacg aacgggatcg tcccaccctg gccgacgaag agaggatctc
     1201 gtccccacgc ctccgtgaac gccgaggagg cggcctcgaa gacgtcgccc ttcggttgaa
     1261 ccgcgaacgg gcgcgcagcc gcgccccgct cgacccggac ctcggccccc cacggcgcgt
     1321 tggcctcgag gtgagcgacg agggcgtcca tcgcgcgctc gggatcgtcg ccgggcgcga
     1381 cgcgcaggct caccttcgcg cgcgcggcag gaaccagcgt gttggacgcc tccaccacgg
     1441 tcggcgcatc gatcccgagg accgagatcc acgggcgacg ccagagccgt gaggtcagcg
     1501 tcccctcgcc gagcgcccgc aggctcggca ccgcgctcgt ctgctcacgc agctcgtcct
     1561 cagtg
//
LOCUS       DASZOB010000208         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125364, whole genome shotgun sequence.
ACCESSION   DASZOB010000208 DASZOB010000000
VERSION     DASZOB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125364"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VG709_03400"
     CDS             complement(<1..692)
                     /locus_tag="VG709_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32151.1"
                     /translation="MATVSDEIAALRGTRPATFGTLLRDRAFSRLWRAILVSSLGDWV
                     GFVAVVALVTRLGGNRSGFAVAGVMLARLLPSIVFGPFAGVLVDRFDRKRLMIIADVV
                     RGVGYASLPFFGTLPAIYAMSFLIECLSLLWTPARDAIIPNLVPRRQLANANTVALAT
                     GYGTLPLGGIVFTALTAVAAAVGTRIAYFEQNPESIAFWLDALTFGFSAWMIAGVPIG
                     RMGRPTRRTAETG"
     gene            complement(692..>1732)
                     /locus_tag="VG709_03405"
     CDS             complement(692..>1732)
                     /locus_tag="VG709_03405"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWC32152.1"
                     /translation="LLRATGQTMKFDGFIRVYTEGRDDEIDEDAESRLPDLSAGAALR
                     LLQVLPEQHFTQPPPRYTEASLIKKLEELGIGRPSTYSSIVSTLLDREYVRLEDRRFY
                     PEDVGEVTTDVLVGRFGDHFLDYDFTARMEGELDDIAEGKMRWTQVLDEFHAPFERKI
                     EKAEEELERPEEELDELCPLCPEEGREPGRLVIKLGRRGKFVGCKNYPDCRYTRDLSG
                     EQRPEPELLDETCPECGRQLMSRVGRFGPFVGCSGYPECKYIKKEQRGTGVTCPECGE
                     GELVQRRTRRGGRVFYGCNRYPSCRFTVGQRPVTDACPKCGGLMVQDGDGVKCTNGDY
                     ASGEGGGPTEAA"
BASE COUNT          280 a          610 c          579 g          263 t
ORIGIN      
        1 ccggtctcgg cggttcgcct cgtggggcgt cccatcctcc cgatcgggac gccggcgatc
       61 atccacgccg agaacccgaa cgtgagcgcg tcgagccaga acgcgatcga ctcggggttc
      121 tgttcgaagt acgcgatccg cgtcccgacg gccgccgcga ccgcggtgag cgccgtgaag
      181 acgatgccgc cgagcggaag cgtcccgtag ccggtcgcta gcgccacggt gttcgcgttc
      241 gcgagctgac ggcgagggac gaggttcggg atgatcgcgt cccgcgcggg cgtccacagg
      301 agcgagaggc actcgatcag gaacgacatc gcgtagatcg cgggaagcgt tccgaagaac
      361 ggaagggacg cgtacccgac ccctcgtacg acgtccgcga tgatcatgag ccgcttccga
      421 tcgaagcggt cgacgagcac gccggcgaag gggccgaaga cgatcgacgg gaggagcctc
      481 gccagcatca cgccggcgac cgcgaaaccc gagcggtttc ccccgagccg cgttacgagc
      541 gcgacgaccg cgacgaaacc cacccagtcg ccgagcgacg atacgaggat ggcgcgccac
      601 agccgcgaga aggctcggtc acgaaggagc gtgccgaagg tggccggtct ggttcctcgg
      661 agcgccgcga tctcgtcgct aacggtcgcc actacgcggc ctccgtgggc ccgccccctt
      721 cacccgacgc gtagtcgccg ttcgtgcact tcacgccgtc gccgtcctgc accatcaggc
      781 cgccgcactt cgggcacgcg tcggtgaccg gccgctgtcc gaccgtgaag cgacaggacg
      841 gatagcggtt gcacccatag aagacccgtc cgccgcgccg cgtacgccgc tgcacgagct
      901 ccccttcgcc gcattcgggg cacgtcacgc cggtgccgcg ctgctccttc ttgatgtact
      961 tgcactcggg gtagccggag cagccgacga acggaccgaa ccgccccacg cggctcatga
     1021 gctgtcggcc gcactcgggg cacgtctcgt cgagaagctc gggctcggga cgctgctccc
     1081 ccgagaggtc gcgcgtatag cggcagtcgg gatagttctt gcagccgacg aacttcccgc
     1141 gccggccgag cttgatcacg agcctgccgg gctcgcggcc ctcctcggga cagagcgggc
     1201 agagctcatc gagctcctcc tcgggacgct ctagctcttc ctcggccttc tcgatcttgc
     1261 gctcgaacgg cgcgtggaac tcgtcgagga cctgcgtcca ccgcatcttg ccttcggcga
     1321 tgtcgtcgag ctcgccctcc atgcgggctg tgaagtcgta gtccaggaaa tggtcgccga
     1381 accggccgac gaggacgtcg gtcgtgacct ctccgacgtc ctcggggtag aagcggcgat
     1441 cctcgaggcg cacgtactcg cggtccagga gcgtcgacac gatcgacgag tacgtcgacg
     1501 ggcgaccgat cccgagctcc tcgagcttct tgatcagcga cgcctcggtg taccgcggcg
     1561 gcggctgcgt gaagtgctgc tcgggcagaa cctggagcag ccggagcgcc gcccccgccg
     1621 agagatcggg gagtcgcgac tccgcgtcct cgtcgatctc gtcgtctcgt ccctcggtgt
     1681 acacgcggat gaacccgtcg aacttcatcg tctgccccgt cgcgcgcagg ag
//
LOCUS       DASZOB010000209         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_145667, whole genome shotgun sequence.
ACCESSION   DASZOB010000209 DASZOB010000000
VERSION     DASZOB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145667"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VG709_03410"
     CDS             <1..808
                     /locus_tag="VG709_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWC32153.1"
                     /translation="REEIGGHVIVRTGRRIPGVVPIAGAHYPISVVRMAVGDAYGAKE
                     WREGEGEDEAAFRTERIERRVCRAVAEFSFRHAERTRAKVFGGPKYTVSPTYEGMLKE
                     EMDAAAARHPDVPYEPQLIDATFALLVSSAGDPLVIPALNRDGDILSDLVLPLFGSIA
                     GSESLLVAFDADYAPKALMAEAAHGTAPALFGKNVANPMAMILAAAALLSHSDDEEPR
                     TAGRAIREAALEAVAAGVRTADLAGHASTSEFTDEVIRRTQAKLQVWATL"
     gene            complement(819..1157)
                     /locus_tag="VG709_03415"
     CDS             complement(819..1157)
                     /locus_tag="VG709_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32154.1"
                     /translation="MDERTRRLTENELLFREVNERIDDVASGLGGEETVYEFVCECSR
                     PDCVDRVSLTLGQYGRIRARPLLFILVDGHEVTEIERVVDTVAPGVVAVEKIGDAASL
                     VAERARRTDR"
     gene            complement(1170..1481)
                     /locus_tag="VG709_03420"
     CDS             complement(1170..1481)
                     /locus_tag="VG709_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWC32155.1"
                     /translation="MTYIIGEPCIDIKDKSCVDVCPVDCIHEADRMLVIDPEECIDCG
                     ACEPECPVEAIYPEDALPEKWEPFVKINYAYGDGMDVVNRLAEEYATEHDVQNPPLDQ
                     R"
     gene            complement(1492..1800)
                     /locus_tag="VG709_03425"
     CDS             complement(1492..1800)
                     /locus_tag="VG709_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HWC32156.1"
                     /translation="MATILVAGVDLFFRGKLDALLGQDHKLTATDSVETPDLVIVDIA
                     RVDPEEVAEEYPDVPILGFTNHTDTKGLRAAHAAGFDRVVVRSALAERARELVAELVT
                     "
     gene            1851..>2126
                     /locus_tag="VG709_03430"
     CDS             1851..>2126
                     /locus_tag="VG709_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWC32157.1"
                     /translation="MAASVESAYELGLDLIRREEYFAAHEALEEAWRAATPAERDFFQ
                     GLVHVAVAWHHARVTGRQVACERQLEKAARRLAPYAPAHRGLDVAGVL"
BASE COUNT          302 a          767 c          747 g          310 t
ORIGIN      
        1 ccgcgaggag atcggcggcc acgtcatcgt ccggaccggc cgccggatcc cgggcgtcgt
       61 cccgatcgcc ggcgcgcact acccgatctc ggtcgtccgc atggcggtcg gcgacgcgta
      121 cggcgccaag gagtggcggg agggcgaggg cgaggacgag gccgccttcc ggacggagcg
      181 catcgagcgg cgcgtctgcc gcgcggtggc ggagttctcc ttccgccacg cggagcgcac
      241 gcgcgcgaag gtcttcggag gcccgaagta cacggtgtcg ccgacgtacg aggggatgct
      301 gaaggaggag atggacgccg cggcggcgcg ccacccggac gtcccgtacg agccccagct
      361 catcgacgcc acgttcgccc tcctcgtcag ctcggcgggg gacccgctcg tgatccccgc
      421 gctcaaccgt gacggcgaca tcctgagcga cctcgtcctc ccgctcttcg gctcgatcgc
      481 gggctccgag tcgctcctcg tcgcgttcga cgcggactac gcgccgaagg cgctcatggc
      541 ggaggcggct cacgggacgg cgccggccct cttcggcaag aacgtcgcga acccgatggc
      601 gatgatcctc gccgccgcgg cccttctctc gcactccgac gacgaagagc cccgcacggc
      661 cggccgcgcg atccgcgagg cggccctcga ggcggtcgcg gccggcgtgc ggaccgccga
      721 cctcgccggg cacgcgtcga cgagcgagtt cacggacgag gtgatccgcc ggacgcaggc
      781 gaagctgcag gtctgggcga cgctctgagc gcagacggct agcggtccgt tcgcctcgcc
      841 cgctcggcga cgaggctcgc cgcgtcgccg atcttctcca cggcgacgac gcccggtgcg
      901 accgtatcga cgacgcgctc gatctccgtg acctcgtgac cgtcgacgag gatgaagagg
      961 agcggccggg cgcggatgcg gccgtactgg cccaacgtca gcgacacccg gtcgacgcag
     1021 tccggccgcg agcactcgca cacgaactcg tagacggtct cctcgccgcc gagtccgctc
     1081 gccacgtcgt cgatccgctc gttcacctcg cggaagagga gctcgttctc ggtcagacga
     1141 cgcgtgcgct cgtccacggc cccttcgccc tagcgctggt cgagcggagg gttctggacg
     1201 tcgtgctcgg tcgcgtactc ctccgcgagc cggttcacca cgtccatgcc gtcgccgtag
     1261 gcgtagttga tcttgacgaa gggctcccat ttctcgggga gagcgtcttc cgggtagatc
     1321 gcctcgaccg ggcactcggg ctcgcatgcc ccgcagtcga tgcactcctc cgggtcgatg
     1381 acgagcatgc ggtccgcctc gtggatgcag tccaccgggc agacgtccac gcaggacttg
     1441 tccttgatgt cgatacaggg ctcgccgatg atgtaggtca cgcgaccgag tctacgtgac
     1501 gagctctgcg acaagctcgc gcgcgcgctc ggcgagcgcc gaccggacga cgacgcgatc
     1561 gaagccggcc gcgtgcgccg cacgcaggcc tttcgtgtca gtgtggttcg tgaagccgag
     1621 gatgggaacg tcggggtact cctcggcgac ctcttcggga tcgacgcggg cgatgtcgac
     1681 gatgacgagg tcgggcgtct cgacgctgtc cgtcgccgtc agcttgtggt cctgccccag
     1741 caacgcgtcc agcttcccgc ggaagaagag atcgacccca gccacgagga tcgtcgccac
     1801 ggcgcgagga gcgtaccacg gacccgcttg tttgctagcg tcgcggcgcg gtggcggcga
     1861 gcgtcgagtc ggcctacgag ctcgggctcg acctgatccg gcgagaagag tacttcgccg
     1921 cgcacgaggc gctcgaggaa gcgtggcgcg cggcgacgcc ggccgagcgc gacttcttcc
     1981 agggcctcgt ccacgtcgcg gtcgcctggc accacgcccg cgtcaccggg cgccaggtcg
     2041 cctgcgagcg ccagctcgag aaggcagcgc gccggctcgc gccgtacgcg cccgcccacc
     2101 gcggcctcga cgtcgcgggc gtcctc
//
LOCUS       DASZOB010000210         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_4709,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000210 DASZOB010000000
VERSION     DASZOB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4709"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VG709_03435"
     CDS             complement(<1..771)
                     /locus_tag="VG709_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HWC32158.1"
                     /translation="MTRDVELLRTPLEDEHAVLGAKMGAFGGWAMPIEYRGVLAEHRA
                     VREAVGAFDLTHLGKVDVEGPGATAWLQQVLTNDLGRLGVGDAQYNLVLTEGGGIVDD
                     LIAYRVADERWLVVPNAANTEKILAILASETRRDVVVTHRPDLTTMAVQGPRSPEVVG
                     RFFDIDGLEYMHCAEGSYRDVLVVVARSGYTGERGYELFAPDAVARPLWREVVEAGRA
                     VGLEPCGLGARDTLRLEMGYPLHGNDISENRTPVEAMLT"
     gene            840..>1549
                     /locus_tag="VG709_03440"
     CDS             840..>1549
                     /locus_tag="VG709_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC32159.1"
                     /translation="MPGDSYDVVVIGSGTGGYSAAIRAAELGKRVAIVERDERLGGTC
                     LLRGCIPSKALLESAAVMDRIERSEDWGIKASGEADWSKVLDFERKIVDKKVSGLTGL
                     IKARKIDVIQGEGKLGAGPSVQVGDRTLQAADVVLATGSRPRLLPGLEVTERIITSDQ
                     ALTMERLPESVVIIGAGAIGLEFATVYRSFGTDVTVLEALPRIAPLEDEDVSKEAARA
                     FKKRGINAMAGVKVQDVK"
BASE COUNT          249 a          516 c          566 g          218 t
ORIGIN      
        1 cgtcagcatc gcctcgaccg gcgtccggtt ctcggagatg tcgttaccgt gcagcgggta
       61 gcccatctcg agccggagcg tgtcgcgcgc gccgagcccg cacggctcga gccccaccgc
      121 gcggcccgcc tccacgactt cgcgccacag aggacgagcg acggcgtcgg gggcgaacag
      181 ctcgtacccg cgctcgccgg tgtacccgga gcgcgccacc acgacgagga cgtcgcggta
      241 cgagccctcg gcgcagtgca tgtactcgag gccgtcgatg tcgaagaatc gcccgacgac
      301 ctccggagac ctcggcccct gaaccgccat cgtggtgagg tccgggcggt gcgtcacgac
      361 gacgtcgcgc cgcgtctcgg aggcaaggat cgcgaggatc ttctcggtgt tcgcggcgtt
      421 ggggacgacg agccaccgct cgtccgcgac gcggtacgcg atgaggtcgt cgacgatgcc
      481 gccgccctcg gtcaggacga ggttgtactg ggcgtctccc accccgagtc ggccgaggtc
      541 gttcgtgagg acctgctgga gccaggcggt cgcgcccggc ccctcgacgt cgaccttgcc
      601 gagatgggtc aggtcgaagg ccccgacggc ctcgcgaacc gcgcggtgct ccgcgaggac
      661 acctcggtac tcgatcggca tcgcccaccc gccgaacgcc cccatcttcg cgccgagcac
      721 ggcgtgctcg tcctcgagcg gcgtgcggag gagctcgacg tcacgcgtca tgggggtggc
      781 ggtagtatga accgagccgg gtaagagcaa cctcaacgag cggcgaaggg aagtcgggaa
      841 tgccgggaga ctcctacgac gtcgtcgtca tcggctccgg caccgggggc tactccgcag
      901 cgatcagggc cgccgagctc ggcaagcgcg tcgccatcgt cgagcgcgac gagcggctcg
      961 gcgggacgtg cttgctgcgc ggctgcatcc cgtcgaaggc gcttctcgag tcggcggcgg
     1021 tgatggaccg catcgagcgc tccgaggact ggggcatcaa ggcgagcggc gaagcggact
     1081 ggtcgaaggt gctcgacttc gagcgcaaga tcgtcgacaa gaaggtctcg gggctcaccg
     1141 gcctcatcaa ggcgcgcaag atcgacgtga tccagggcga ggggaagctc ggcgccggtc
     1201 cgtcggttca ggtcggtgac cgcacgctgc aggccgccga cgtcgtactc gcgacggggt
     1261 cgagaccgcg gctgctgccg gggctcgagg tgaccgagcg catcatcacg agcgaccagg
     1321 cgctcacgat ggagcggctc ccggaatccg tcgtgatcat cggggccggc gcgatcgggc
     1381 tggagttcgc gacggtgtac cgctcgttcg ggaccgacgt caccgtgctc gaggcgctgc
     1441 cgcgcatcgc gccgctcgag gacgaggacg tctcgaaaga agcggcgcgt gccttcaaga
     1501 agcgtggcat caacgcgatg gccggagtca aggtgcagga cgtgaagga
//
LOCUS       DASZOB010000211         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_105858, whole genome shotgun sequence.
ACCESSION   DASZOB010000211 DASZOB010000000
VERSION     DASZOB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105858"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2567)
                     /gene="metH"
                     /locus_tag="VG709_03445"
     CDS             complement(<1..>2567)
                     /gene="metH"
                     /locus_tag="VG709_03445"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWC32160.1"
                     /translation="EAGGRFVAGSVGPLNQTLSLSPRVDDPSFRAVSFDQVRDAYAEQ
                     IRALRDGGVDLLLVETVFDTLNAKAAIVAAKEEAPELPLWISVTIVDRSGRTLSGQTL
                     EAFWTSVEHARPLVVGVNCALGAREMRPYIEELSRLADTFTSCHPNAGLPNAFGGYDE
                     RPDTTSSLLREFAADGLVNVVGGCCGTTPDHVRAIVDATRALSPRHVPKRQPVTRFSG
                     LEPFTIFEDASFVMIGERTNVTGSARFRRLVEAGNYQGALDVALEQVRGGANILDVNM
                     DADLLESEAAMTTFLNLVATEPEVARLPIMIDSSRWSVIQAGLRCVQGKSIVNSISLK
                     EGEEDFLVKAREARRFGAAVVVMAFDEQGQAETVARKVEICARAYDLLVREVGFPPED
                     LVFDPNVLAVATGMEEHNRFAKEFIEAVPLIKERCPGARVSGGISNLSFSFRGNEVVR
                     EAIHSAFLYHAIAAGLDMGIVNAGQLVVYEDIPPDLLERVEDIIFDRRPDATERMVAF
                     AESVRGEGTKREIDLSWREAPVEERLSYALVHGIVDFIEEDTEEARQRHARPLDVIEG
                     PLMQGMQIVGDLFGSGKMFLPQVVKSARAMKRAVAYLEPYMEAEKARGNGRAQAKVVL
                     ATVKGDVHDIGKNIVGVVLGCNSYAVVDLGVMVPADRILDTAESEGCDVVGLSGLITP
                     SLDEMVNVAKEMERRGMSLPLLIGGATTSKQHTAVKIAPAYGQPTVHVLDASRVVKVV
                     SSLLDGERRGVLDRENRELQDRLREQHGERERKPLLPYERARANRTPIAWAAADVAVP
                     AFTGTRVVEPDVATLREYVDWQVFFHAWELKGKFPKLLDDPEKGATARELYAHANEL"
BASE COUNT          370 a          923 c          865 g          409 t
ORIGIN      
        1 gagctcgttc gcgtgcgcgt agagctcacg cgcggtcgcg cccttctcgg gatcgtcgag
       61 gagcttgggg aactttccct tgagctccca cgcgtggaag aagacctgcc agtcgacgta
      121 ctcgcgcagc gtcgcgacgt ccggctcgac gacgcgcgtt ccggtgaagg cgggcacggc
      181 gacgtcggca gccgcccagg cgatgggcgt ccggttcgcg cgcgcccgct cgtacggcag
      241 gagcggcttg cgctcgcgct cgccgtgctg ctcgcggagg cgatcctgga gctcgcggtt
      301 ctcgcggtcg aggaccccgc ggcgctcgcc gtcgaggagg ctcgacacga ccttcaccac
      361 gcgtgaggcg tcgagcacgt gcacggtcgg ctgcccgtag gcgggcgcga tcttgaccgc
      421 cgtgtgctgc ttcgacgtcg tcgcgcctcc gatcaggagc ggaagcgaca tgccgcgccg
      481 ctccatctcc ttcgccacgt tgaccatctc gtcgagggac ggcgtgatca ggcccgagag
      541 cccgacgacg tcacagccct cgctctcggc cgtgtccagg atgcggtccg ccggcaccat
      601 cacgccgagg tcgaccacgg cgtagctgtt gcagccgaga acgacgccga cgatgttctt
      661 gccgatgtcg tggacgtcgc ccttgaccgt cgcgaggacg accttcgcct gagcccgccc
      721 gttcccgcgc gccttctcgg cctccatgta cggctcgagg taggcgacgg cgcgcttcat
      781 cgctcgcgcg ctcttcacaa cctgcggcag gaacatcttc cccgacccga agaggtcgcc
      841 gacgatctgc atgccctgca tgagcggccc ctcgatcacg tcgagcgggc gggcatgccg
      901 ctgccgggcc tcctcggtgt cctcctcgat gaagtcgacg atcccgtgga cgagcgcgta
      961 cgagaggcgc tcctccacgg gcgcctctcg ccacgacagg tcgatctccc gcttcgttcc
     1021 ctcgccccgc acgctctcgg cgaaggcgac catgcgctcg gtcgcgtcgg ggcggcggtc
     1081 gaagatgatg tcctcgacgc gctcgagaag gtcgggcggg atgtcctcgt agaccacgag
     1141 ctgccccgcg ttcacgatcc ccatgtcgag cccggcggcg atcgcgtgat agaggaaggc
     1201 cgagtggatc gcctcgcgga cgacctcgtt gccgcggaag gagaaggaga ggttcgagat
     1261 gccgcccgac accctcgctc cggggcagcg ttccttgatg agcgggaccg cctcgatgaa
     1321 ctccttcgcg aagcggttgt gctcctccat cccggtcgcg accgcgagga cgttcggatc
     1381 gaacaccagg tcctcgggcg ggaagccaac ctctcggacg aggaggtcgt aggcgcgtgc
     1441 gcagatctcg accttgcgcg cgaccgtctc ggcctggccc tgctcgtcga acgccatcac
     1501 gacgacggcc gcgccgaacc gcctcgcctc gcgcgccttc acgaggaagt cctcctcgcc
     1561 ctccttcagg ctgatcgagt tcacgatcga cttgccctgg acgcacctga ggcccgcctg
     1621 gatcacggac cagcgggagc tgtcgatcat gatcggcagc cgcgcaacct cgggctccgt
     1681 cgcgacgagg ttgaggaacg tcgtcatcgc cgcctccgac tcgagcaggt cggcgtccat
     1741 gttcacgtcg aggatgttcg ccccgccgcg gacctgctcg agcgcgacgt cgagggctcc
     1801 ctggtagttc ccagcctcga cgaggcggcg gaaccgggcc gaccccgtca cgttcgtccg
     1861 ctcgccgatc atgacgaagc tcgcgtcctc gaagatcgtg aacggctcga ggccgctgaa
     1921 cctcgtaact ggctgcctct tcgggacgtg tctcggggac agtgccctgg tcgcgtccac
     1981 gatcgccctc acgtggtcgg gcgtcgtccc gcagcacccg ccgacgacgt tcacgagccc
     2041 gtccgccgca aactcgcgga ggagcgagct cgtcgtgtcg ggccgctcgt cgtagccgcc
     2101 gaacgcgttc gggagccccg cgttcgggtg gcagctcgtg aaggtgtcgg caagccggga
     2161 cagctcctcg atgtacggcc gcatctcgcg cgcgccgagc gcgcagttga cgccgaccac
     2221 gagcggccgc gcgtgctcga ccgacgtcca gaacgcctcg agcgtctggc ccgagagcgt
     2281 gcgcccgctc ctgtcgacga tcgtgaccga gatccagagc gggagctccg gcgcctcctc
     2341 ctttgccgcg acgatggccg ccttcgcgtt cagcgtgtcg aagaccgtct cgacgaggag
     2401 caggtcgacg ccaccgtcgc ggagcgcgcg gatctgctcg gcgtacgcgt cgcggacctg
     2461 gtcgaagctc accgcccgga acgacgggtc gtcgacgcgg ggcgagagcg agagcgtctg
     2521 gttgagcggg ccgaccgacc cggccacgaa gcggccgccc gcctcgt
//
LOCUS       DASZOB010000212         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_95349, whole genome shotgun sequence.
ACCESSION   DASZOB010000212 DASZOB010000000
VERSION     DASZOB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95349"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="coaBC"
                     /locus_tag="VG709_03450"
     CDS             complement(<1..910)
                     /gene="coaBC"
                     /locus_tag="VG709_03450"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWC32161.1"
                     /translation="MPPDGRAAARVLVGVTGSIAAYKACELVRLLVQDGTEVVPLLTP
                     DAERFVSAHTFEALAGRPVPQDVYPHLVQADLLVVAPLSANTLAKLAHGFADNVLTQA
                     ALAFRGPVLLAPAMNARMWEHPASQESVRLLRERGAELVGPETGELAEGEVGPGRMAE
                     PHAIAERCRALLARRGADDPLRGRRVLVTAGGTREPLDSVRFLGNRSSGRMGVALADE
                     ARRRGADVTLLAANLAVPAPTGVEVVETPTAADLAHEALARADADVVLMAAAVADYRP
                     TERVAGKRAKSGEPWTVTLEPTTDVLA"
     gene            complement(951..1181)
                     /gene="rpoZ"
                     /locus_tag="VG709_03455"
     CDS             complement(951..1181)
                     /gene="rpoZ"
                     /locus_tag="VG709_03455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWC32162.1"
                     /translation="MIHPRIDELLGETGSRYGLVIAAAKRARQINNYHHQLGEGTFED
                     VAPPLVESRSKNYLTMALEEIAEGKIVYSSRD"
     gene            complement(1519..2286)
                     /gene="gmk"
                     /locus_tag="VG709_03460"
     CDS             complement(1519..2286)
                     /gene="gmk"
                     /locus_tag="VG709_03460"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWC32163.1"
                     /translation="MPHQPVEDGGRPFGAAARRARELAAPLNEAISRDGTRRASERQT
                     ARGRLVVVTGPSGAGKGTLIRRLVRRRPDLEVAVSATTRPRRKGEIDGRDYHFLDDPA
                     FDEGLKRGDFLEHVPYVSGHRYGTLHSEVARIVEAGRTCILELETAGARRVKELRPET
                     VTVFVAVPSFAELERRLRDRATESAGEIEERLDLARQQTEEAREFDHVVVNDDVERAV
                     DRLDELISESAAASSSSAGADAPGQPRSRSNSAPSAG"
BASE COUNT          298 a          853 c          876 g          349 t
ORIGIN      
        1 cggcgaggac gtccgtggtg ggctcgagcg tcacggtcca cggctccccg ctcttcgcgc
       61 gcttgcccgc gacgcgctcg gtcggtcgat agtcggccac tgccgcggcc atcaggacga
      121 cgtctgcgtc ggcgcgcgcg agggcctcgt gcgccaggtc ggccgcggtc ggcgtctcga
      181 cgacctcgac gccggtcggc gccgggaccg cgaggttcgc cgcgaggagc gtcacgtcgg
      241 caccgcggcg acgcgcctcg tcggcgagcg cgactcccat gcggccgctc gagcggttgc
      301 cgaggaagcg gacggagtcg agcggctccc tggtcccgcc ggccgtcacg aggacgcgtc
      361 ggccgcggag cgggtcatcc gcgcctcgtc gcgcgaggag cgcgcggcac cgctccgcga
      421 tcgcgtgcgg ctccgccatg cgtccgggcc cgacctcacc ttcggcgagc tctcccgtct
      481 cggggccgac gagctcggcg ccgcgctcgc gcaggaggcg gacgctctcc tgcgacgcgg
      541 ggtgctccca catgcgcgcg ttcatcgccg gtgcgaggag gaccggcccg cggaaggcga
      601 gggcggcctg tgtcaggacg ttgtccgcga agccgtgggc gagcttggcg agcgtgttgg
      661 ccgagagcgg cgcgacgacg aggaggtcgg cctggacgag gtgcggatac acgtcttgcg
      721 ggacgggacg cccggcgagc gcctcgaagg tgtgcgccga gacgaaccgc tcggcgtccg
      781 gagtgaggag cggaacgacc tcggtgccgt cctggacgag gagccggacg agctcgcagg
      841 ccttgtaggc cgcgatcgac ccggtgacgc ccacgaggac ccgcgcggct gcgcggccgt
      901 ctggcggcac gcccggtgag cctcgctcct ccacgctcgc gcgagcgacg ctagtcgcgc
      961 gagctgtaga cgatcttgcc ctcggcgatc tcttcgaggg ccatcgtgag gtagttcttc
     1021 gagcgggact cgacgagggg cggcgcgacg tcctcgaagg ttccttcgcc cagctggtgg
     1081 tggtagttgt tgatctgcct ggcgcgcttg gcggcggcga tcacgagccc gtagcgggag
     1141 ccggtctcgc cgagcagttc gtcgatgcgc gggtggatca tcggcgatca gtgtagcggc
     1201 cggacatgca tccccgtggc gcggctagcc ggccaccgcg ggtgagccgt cccgccgggc
     1261 acgcgcgaac ggcttccgag cacgcgggcg gtcgaggtcg ccggcccccc gcggtcgacg
     1321 gggtcgcgtg gtcgcagcgc tcccccgccc gaggcccgcg cggcgcacga agagaagccg
     1381 cgctgtcgcc gcttcttccg tcgctcgtgc cgacaatgtg cccctggggg acccccctgt
     1441 ggttccccct cgcgactcag cgtaccgccg aaaagcgcat ctgtgtggcg cgggcatgcg
     1501 ccgcaagtgt gacgctcgct accccgcaga aggtgccgag ttgctgcggc tccgtggctg
     1561 gccgggcgcg tctgcgccgg ccgacgagga ggatgcggcc gccgactcgc tgatcagctc
     1621 gtcgagccgg tcgacggccc gctccacgtc gtcgttcacg acgacgtgat cgaactcacg
     1681 agcttcctcc gtctgctggc gggcgaggtc gagtcgctcc tcgatctctc ccgcgctctc
     1741 ggtcgcgcga tcccgcagcc gccgttcgag ctctgcgaac gacggcacgg cgacgaagac
     1801 ggtgaccgtc tccggccgaa gctccttgac gcgacgcgct ccggcggtct cgagctcgag
     1861 aatgcacgtc cggcccgcct cgacgatgcg cgccacctcg gagtgcagcg tgccgtagcg
     1921 gtggcccgac acgtacggca cgtgctcgag gaagtcgccg cgcttaaggc cctcgtcgaa
     1981 cgccggatcg tccaggaagt ggtagtcgcg gccgtcgatc tcgcctttgc gccgcggccg
     2041 cgtcgtggcc gagactgcga cctcgaggtc gggtcgccgc cggacgagcc gccggatcag
     2101 ggtgcccttg ccggccccgg acggccccgt gacgacaacc agcctgccgc gcgccgtctg
     2161 tcgctccgaa gcccggcggg tgccgtctct cgagatcgcc tcgttcagcg gcgcagcaag
     2221 ctcacgagct cggcgcgctg ccgctccgaa aggccgccca ccgtcttcga ctggctgatg
     2281 cggcactggt tgaggaaccg ggccgccttg acgcgtccga acttcgggac ggcgatcagg
     2341 atgtcgatca ccttggccgt ctccacgcac tcgggc
//
LOCUS       DASZOB010000213         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_85271, whole genome shotgun sequence.
ACCESSION   DASZOB010000213 DASZOB010000000
VERSION     DASZOB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85271"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VG709_03465"
     CDS             <1..307
                     /locus_tag="VG709_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWC32164.1"
                     /translation="SVDAPEIATSDDVAAALDALREFLFERVYLRPEQAHEQEKAVEL
                     IRSLFSYYLEHPDEIPAEYASAPGDIPTRVADYIAGMTDRYALRTYERLFLPQGWLL"
     gene            311..2116
                     /gene="dnaG"
                     /locus_tag="VG709_03470"
     CDS             311..2116
                     /gene="dnaG"
                     /locus_tag="VG709_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWC32165.1"
                     /translation="MARIRQEDIEAVRERTDIVRLVQQYIALKKAGRAFSGLCPFHPE
                     KTPSFSVDPGKQVYYCFGCGAGGNAFHFLEQVEGVTFPEAVERLAGPAGITLRYEGVS
                     PGERRQISRKQSLYRANQRAADLYRKMLLEGREAEGARKYLASRGITKESVEAFGVGF
                     APGYPDFLLRRLSRDLSPELLVEAGLAIRDERSVRDRFRARITFPAHDVSGNAVGIGA
                     RLLEGDGPKYLNSPETPVYRKQELLYNLNRAKGDISRSERAFLVEGYTDVIALHQAGV
                     TTAVATCGTAVGEGHFRLLSRFARRVVLAFDSDEAGARAAERAYAFHERYPLQASVLV
                     LPEGVDPADFALGRGGEAFEELASGAAPLVEFMLRRAVRGHDLATPEGRTRAVQAGMP
                     IVAGLDDPVRRSEYAGMLADLAGVDVESVLLELARRPAGGDGAGPGRQRSAEEPGPSR
                     RTAEPRSPDREVEKEALKLLAQHPDLCLGRVSTLEHELFTSERNRKTLALLLEPWSSA
                     SQLMERARDRGVGELVAEIAVEPLKGEVDRTYAAHVFSRLEELSLKRRMDTMRKRLER
                     LNPVKDPDAFDSLFKELADLTSRWRDLQARAGEGA"
     gene            2162..>3054
                     /locus_tag="VG709_03475"
     CDS             2162..>3054
                     /locus_tag="VG709_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC32166.1"
                     /translation="MIVARGRERGFVTSEDLLEGLLVDDLTAEQVEDFLAQIEEHLRE
                     QGIEIIEVPGEELDGGQTTLPRDEELLKAPTNDPVRMYLKEIGKVPLLTAAQEVDFAM
                     RIEAGEAATALLISMYDGGRVDQKRFRQIVELAVRVREHQVEKFGRVDGIGRETVSRR
                     YSPKSRQETDDFLRKVERDGQIAKRKLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNL
                     GLIRAVEKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRIQR
                     QLLQDLGREPLPEEIGEQMGIG"
BASE COUNT          492 a         1039 c         1077 g          446 t
ORIGIN      
        1 gtccgtcgac gctccggaga tcgcgacgtc cgacgacgtc gcggccgcgc tcgacgcgct
       61 ccgcgagttc ctgttcgagc gggtctacct ccgcccggag caggcgcacg agcaggagaa
      121 ggccgtcgag ctgatccggt cgctgttctc gtactacctc gagcatcccg acgagatccc
      181 ggcggagtac gcatccgcgc ccggcgacat cccgacgcgc gtcgccgact acatcgcggg
      241 catgaccgac cgctacgcgc tccgcacgta cgaacggctc ttccttcccc aggggtggtt
      301 gctctaggga atggcacgca tccgccaaga ggacatcgaa gccgtccgcg aacggacgga
      361 catcgtgcgg ctcgtccagc agtacatcgc gctcaagaag gccgggcgcg cgttctcggg
      421 gctgtgccca ttccacccgg agaagacacc gtcgttcagc gtcgaccccg ggaagcaggt
      481 ctattactgc ttcggctgcg gggccggcgg caacgcgttc cacttcctcg agcaggtcga
      541 gggcgtcacg ttccccgagg ccgtcgagcg cctcgcgggt ccggccggca tcacgcttcg
      601 ctacgagggc gtgtcgccgg gcgagcgccg ccagatctca cggaagcaat cgctctaccg
      661 agcgaaccag cgcgccgcgg acctgtaccg caagatgctg ctcgaggggc gggaggccga
      721 gggcgcgcgg aagtacctcg cgtcccgcgg catcaccaag gagtccgtcg aggcgttcgg
      781 cgtcgggttc gcgccgggct accccgactt cctcctgcgg cgtctgtcgc gcgacctctc
      841 gccggagctc ctcgtcgagg cgggcctcgc gatccgcgac gagcgcagcg tgcgcgaccg
      901 cttccgtgcg cgcatcacgt tcccggccca cgacgtgtct ggcaacgccg tcggcatcgg
      961 cgcccgtctg ctcgagggcg acgggccgaa gtacctgaac tcgccagaga cgcctgtgta
     1021 ccggaagcag gagctgctct acaacctcaa tcgcgcgaag ggcgacatct cgcggagcga
     1081 gcgcgcgttc ctcgtcgagg ggtacaccga cgtgatcgcc ctccaccagg ccggggtcac
     1141 gacggcggtc gcgacctgcg ggaccgcggt gggcgagggg cacttccgtc tcttgtcccg
     1201 cttcgcgcgg cgcgtcgtgc tcgcgttcga ctccgacgag gcgggcgcgc gtgccgcgga
     1261 gcgcgcgtac gcgttccacg agcgctatcc gctccaggcc tccgtgctcg tgcttccgga
     1321 gggggtggac cccgcggact tcgctctggg acgtggcggc gaggcgttcg aagagctcgc
     1381 gtcgggcgcc gcgccgctcg tcgagttcat gctgcggcgc gcggttcggg gtcacgatct
     1441 cgcgacgccc gaggggagga cgcgtgcggt gcaggccggg atgccgatcg tggccggcct
     1501 cgacgatccg gtccggcgca gcgagtacgc gggcatgctc gccgacctcg cgggcgtcga
     1561 cgtcgagtcg gtcctgctcg agctcgcgcg gcggccggcc ggtggcgacg gcgccggccc
     1621 gggccggcag cggtcggccg aagagccggg tccatcgcgc cgcaccgccg agcctcgctc
     1681 gcctgacagg gaggtcgaga aggaagcgct caagctgctc gcgcagcatc ccgacctctg
     1741 tctcggtcgc gtgtcgacac tcgaacatga gctgttcacg tcggagcgga accgcaagac
     1801 gcttgccctc ctgctcgagc cgtggagcag cgcgtcgcag ctgatggagc gcgcgcgaga
     1861 ccgaggcgtc ggtgagctcg tcgccgagat cgcggtcgag ccgctcaagg gcgaggtcga
     1921 tcgcacgtac gcggcccacg tcttctcgcg cctcgaggaa ctgtcactca agcgtcggat
     1981 ggatacaatg cggaagcgct tggagcgcct caatcccgtg aaagatcccg acgccttcga
     2041 ctccctcttc aaggagttgg ccgatctcac gagcaggtgg cgcgatcttc aggcgcgcgc
     2101 tggagaagga gcgtaggtgg cggaagtaac gcgagacccg gtcctcgacg aggccaagga
     2161 gatgatcgtc gcgcggggcc gcgagcgcgg cttcgtgacg tcggaggacc tcctcgaggg
     2221 actgctggtc gacgacctca cggccgagca agtcgaggat ttcctcgcgc agatcgagga
     2281 gcacctccgc gagcagggca tcgagatcat cgaggtcccc ggcgaggagc tcgacggcgg
     2341 ccagacgacg ctgccgcgag acgaggagct cctcaaggcg ccgacaaacg acccggtccg
     2401 gatgtacctc aaggagatcg ggaaggtccc gctgctcacg gcggcgcagg aggtcgactt
     2461 cgcgatgcgg atcgaggccg gcgaggccgc gaccgcgctc ctcatctcga tgtacgacgg
     2521 cgggcgcgtc gaccagaagc ggttccggca gatcgtcgag ctcgccgtgc gggtccgcga
     2581 gcatcaggtc gagaagttcg ggcgcgtcga cggcatagga cgcgagacgg tctcgcgccg
     2641 ctactcgccg aagagccgac aggagaccga cgacttcctc cgcaaggtcg agcgcgacgg
     2701 acagatcgcg aagcggaagc tgatcgaggc caacctccga ctggtcgtct cgatcgcgaa
     2761 gcggtacgtg gggcgcggca tgctcttcct cgatctcatc caggaaggga acctcggcct
     2821 catccgcgcc gtcgagaagt tcgactactc gaagggcttc aagttctcga cgtacgccac
     2881 gtggtggatc cgccaagcga tcacgcgagc gatcgcggac caggcgcgga cgatccgcat
     2941 ccccgtgcac atggtggaga cgatcaacaa gctggtgcgg atccagcgcc agctgctgca
     3001 ggacctcggc cgcgagccgc tccccgagga gatcggcgag cagatgggca tcgg
//
LOCUS       DASZOB010000214         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125458, whole genome shotgun sequence.
ACCESSION   DASZOB010000214 DASZOB010000000
VERSION     DASZOB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125458"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1000
                     /locus_tag="VG709_03480"
     CDS             92..1000
                     /locus_tag="VG709_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32167.1"
                     /translation="MARPGDPSGSPAAVSEDAGSIDDVLASLDRPPPRPRDLYYRWER
                     EQWEAGAIDFAPDRSAWPALDPATRTSILAALACVLVPDGRVAELLVPFVDAVATEEE
                     QVFLTTQLVDEARAVVLSDRFFEEVVQGKSKTEKRDEVEPGVGLANRRLDELFAQVAP
                     RAEAVRVDRMPSAELHAGLLLLDVVFEGVVLVAVTRRLCHWLERDAGLDGLRTGLVNL
                     GRDVTRHVLFAEHLIQNADQPEADLAALEGVIEEALPVVRGVVDDAAEASNDFAGLPL
                     TDAELSTETMECLARRVQDVGIDLPT"
     gene            1012..>1637
                     /locus_tag="VG709_03485"
     CDS             1012..>1637
                     /locus_tag="VG709_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWC32168.1"
                     /translation="MTSAASELRAINDVVVSCRACPRLVAWRETVAQEKRAAFAHEDY
                     WGRPLPSFGDPHARLLIVGLAPAAHGGNRTGRIFTGDRSGDWLFASLWRTGYANQPQS
                     LHRDDGLALHDAYIAAAVRCAPPANKPRPEERDRCLLYLIDEVRTLPRLKVVVALGSF
                     AWDAALRVHAAVTGSRVSPKPRFAHGAEADLGKFVLLGSYHPSQQNTFT"
BASE COUNT          233 a          582 c          559 g          263 t
ORIGIN      
        1 agctcggcgg gctcgggacc tacaagttct tctgcaagta ccacgccgag caggacatgc
       61 gcggcgtctt caccgtcgag gagacctcct agtggcgcgg ccgggagatc cgagcggctc
      121 gccggcagcg gtctccgagg acgccgggtc gatagacgac gtcctcgcct ccctcgaccg
      181 gccgccgcca cgcccccgcg acctctacta ccgctgggag cgcgagcagt gggaggcggg
      241 cgcgatcgac ttcgcccccg accggtcggc gtggccggct ctcgatcccg ccacccggac
      301 atcgatcctc gccgcgctcg cgtgcgtcct cgtccccgac ggccgcgtcg ccgagctgct
      361 ggtgccgttc gtggacgccg tcgccaccga ggaagaacag gtcttcctca ccacccagct
      421 cgtcgacgag gcgcgcgcgg tcgtgctctc cgaccgcttc ttcgaggagg tcgtgcaggg
      481 caagtccaaa accgagaaga gggatgaggt cgagccgggg gtcgggctgg ccaatcgccg
      541 gctcgacgaa ctgttcgcgc aggtagctcc gcgggccgag gcggtgcggg tcgatcgcat
      601 gccgtccgcc gagctccacg cggggctcct cctgctcgac gtcgtgttcg agggcgtcgt
      661 cctggtcgcg gtcacccggc gcctgtgcca ctggctcgag cgcgacgcgg gactcgacgg
      721 gctgcgaacg ggactcgtca acctgggacg ggacgtgacg cggcacgtgc tcttcgccga
      781 gcatctgatc cagaacgcgg atcagcccga ggcggatctc gcggcgctgg agggtgtcat
      841 cgaagaggcg ttgcccgtcg tgcggggcgt cgtcgacgac gctgccgagg cgtcgaatga
      901 cttcgcgggg cttcctctga ccgatgcgga gctttccacg gagacgatgg aatgcctcgc
      961 gcgccgcgtc caggacgtcg gcatcgatct cccgacctga agcgcgctta catgacctcc
     1021 gccgcgtccg agctgcgcgc gatcaacgac gtcgtcgtct cttgccgcgc ctgtccgcga
     1081 ttggtcgcgt ggcgggaaac cgtcgcccag gagaagcggg ccgccttcgc gcatgaggac
     1141 tactggggcc gtcccttacc gagtttcggc gacccgcacg cgcggctgct gatagtcggc
     1201 ctggccccgg cggctcacgg aggaaaccgc acggggcgca tcttcaccgg cgaccgttcg
     1261 ggcgactggc tctttgcctc gctgtggcgg accggctatg ccaaccagcc gcagtcgctc
     1321 caccgcgacg acggactcgc gctgcacgac gcctatatcg cggccgccgt gcgctgcgcg
     1381 cctcccgcga acaaaccgcg gcccgaagag cgcgatcgct gtcttcttta tctgatcgac
     1441 gaggttcgaa cgctgccgcg cctgaaggtg gtcgtggcgc tgggctcctt tgcgtgggac
     1501 gcggccttgc gcgtccacgc ggccgtaacc ggaagccggg tctcgcccaa accgcgcttc
     1561 gcgcacggcg ccgaggcgga cctcggcaag ttcgttctgc tcggttcgta tcaccccagt
     1621 cagcagaaca ccttcac
//
LOCUS       DASZOB010000215         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125487, whole genome shotgun sequence.
ACCESSION   DASZOB010000215 DASZOB010000000
VERSION     DASZOB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125487"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VG709_03490"
     CDS             complement(<1..667)
                     /locus_tag="VG709_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWC32169.1"
                     /translation="MLRFATAGSVDDGKSTLIGRLLYDTKALFDDQLEAVERASEQRG
                     DAYTDLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHIQYTRNMVTGAST
                     ADLAVVLIDARKGVVEQSRRHAFISTLLRIPHLVVAINKMDLVDYDEAVYEEIAEEFS
                     SWASRLEAPDITFIPISALHGDNVVAPSDNMPWYKCTPLLYHLEHVHIGSDRNLIDAR
                     LPVQ"
     gene            complement(721..>1516)
                     /gene="cysD"
                     /locus_tag="VG709_03495"
     CDS             complement(721..>1516)
                     /gene="cysD"
                     /locus_tag="VG709_03495"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWC32170.1"
                     /translation="DSIVLLRLAEKAFWPGRFPFPVMHVDTGHNFPEVIEFRDRRIEE
                     LGARLVVASVQESIAKGRVREESGPRASRNRLQTTTLLDAIQEHGFDACFGGARRDEE
                     RARAKERVFSFRDDFGQWDPKRQRPELWSLYNGRIRKGEHVRVFPLSNWTELDVWQYI
                     AEERLEIPPIYFAHERAVFERDGMLYAASDFVELLDGEEPFTASVRYRTVGDMSCTGA
                     VRSSAATLEEVVAEIATTRVPERGETRAADRVTEAAMEDRKREGYF"
BASE COUNT          223 a          510 c          543 g          240 t
ORIGIN      
        1 actggacggg gaggcgggcg tcgatcaggt tgcggtcgga gccgatgtgg acgtgctcga
       61 ggtggtacag cagcggcgtg cacttgtacc agggcatgtt gtccgagggc gccacgacgt
      121 tgtcgccgtg gagggcggag atcgggatga acgtgatgtc gggggcctcg agccgagagg
      181 cccagctcga gaactcctcg gcgatctcct cgtagaccgc ctcgtcgtag tcgacgaggt
      241 ccatcttgtt gatcgcgacg acgaggtgcg ggatgcgcag gagcgtggag atgaacgcgt
      301 gccgccgcga ctgctcgacg accccctttc gcgcgtcgat cagcacgacg gcgaggtcgg
      361 ccgtcgaggc gcccgtgacc atgttgcgcg tgtactggat atgccccggc gtgtcggcga
      421 tgatgaactt gcgccggggc gtcgcgaagt agcggtaggc gacgtcgatg gtgatcccct
      481 gctcgcgctc ggcgcgcagc ccgtcggtga ggagcgcgag gtcggtgtag gcgtcaccgc
      541 gctgctcgct cgcgcgctcg acggcctcga gctgatcgtc gaacagcgcc ttggtgtcgt
      601 acaggagccg gccgatcaac gtcgacttgc cgtcgtccac cgagccggcg gtggcgaagc
      661 gcagcatctc ggtctgccgc acgccggtca gcaccccgtt cgctcccgcg cccggcacgg
      721 ctagaagtac ccctcccgct tgcggtcctc catcgccgcc tcggtcacgc ggtcggcggc
      781 gcgcgtctcg ccgcgctcgg gcacccgcgt ggtggcgatc tccgcgacca cctcctcgag
      841 cgtggcggcg ctcgagcgga cggcaccggt gcagctcatg tcgccgaccg tgcggtaccg
      901 cacggacgcg gtgaacggct cctcgccgtc gagaagctcg acgaagtcgc tcgccgcgta
      961 cagcatcccg tcgcgctcga agaccgcgcg ctcgtgggcg aagtagatcg gcggtatctc
     1021 gagccgctcc tcggcgatgt actgccacac gtcgagctcg gtccagttcg acagggggaa
     1081 gacgcgaacg tgctcgccct tgcggatccg cccgttgtag aggctccaca gctcggggcg
     1141 ctggcgcttc gggtcccact ggccgaagtc gtcgcggaac gagaagacgc gctccttggc
     1201 gcgcgcccgc tcctcgtcgc ggcgcgctcc gccgaagcag gcgtcgaagc catgctcctg
     1261 gatggcgtcg agcagcgtcg tggtctgaag ccgattgcgc gaggcacgcg ggccgctctc
     1321 ctcacgcacg cgccccttgg cgatcgactc ctgcaccgag gcgacgacga gccgggcgcc
     1381 aagctcctcg atccggcggt cgcggaactc gatcacctcg gggaagttgt ggcccgtgtc
     1441 gacgtgcatg accgggaacg ggaagcgacc cggccagaag gccttctcgg ccagacgcag
     1501 gaggacaatc gagtcc
//
LOCUS       DASZOB010000216         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173903, whole genome shotgun sequence.
ACCESSION   DASZOB010000216 DASZOB010000000
VERSION     DASZOB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173903"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..347)
                     /gene="secE"
                     /locus_tag="VG709_03500"
     CDS             complement(60..347)
                     /gene="secE"
                     /locus_tag="VG709_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWC32171.1"
                     /translation="MNRQAKRMLQRQQATQDRVAAGRRPVPERKRRVGPRQFLREVRQ
                     ELKKVAWPTRREMVAYTTVVLVTVIVLTSLVFGFDFVISRAVLFVCGGQAG"
     gene            complement(361..433)
                     /locus_tag="VG709_03505"
     tRNA            complement(361..433)
                     /locus_tag="VG709_03505"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Trp,seq:cca)
     gene            570..1046
                     /locus_tag="VG709_03510"
     CDS             570..1046
                     /locus_tag="VG709_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32172.1"
                     /translation="MQQIKEPAAIGSPSRRVPPRRRIPIPDPEVGALADRIDRLEAVL
                     RQAVRANKEVLRTLDPARSESDAAARRRELAAFGDRVREGLTGVARVIRAELRATQER
                     QDDVRRDVAELREAVESLSRQLGDRRAPERAPVTRLADVRLRLSRITDELAADVAP"
     gene            complement(1047..1364)
                     /locus_tag="VG709_03515"
     CDS             complement(1047..1364)
                     /locus_tag="VG709_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32173.1"
                     /translation="MREDIDVHLATCPECSGELARYRELMGALASLRDDVEIAPVGMP
                     ERIVASVLSPRARIRAAAARAAQDRRVHVAAASRGGALVGGAAIALVWWRIARRGLVA
                     RAA"
     gene            complement(1357..1938)
                     /locus_tag="VG709_03520"
     CDS             complement(1357..1938)
                     /locus_tag="VG709_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWC32174.1"
                     /translation="MSRELLEACRRGDPRAFEELVETTHRRVYTLAYRLVGDRQEAED
                     VAQEAYLRAHRSLRGFRGDARFETWMYRIVSNVAMTHLRRRKRFGDLVADDSGGPEPE
                     VRPTEDLVERDELKRALEALPLGQRTVVVLKDVYGLSCQEIGDQIGVSEGAVKVRLHR
                     ARKRLKDLIYGDAPEGHRDVRERLPEYVEEGSA"
     gene            complement(1988..2407)
                     /locus_tag="VG709_03525"
     CDS             complement(1988..2407)
                     /locus_tag="VG709_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWC32175.1"
                     /translation="MARFEDVAVGDEVFSVPKVIRREDVKAYADASGDQNPLHQDDDF
                     ARSVGFPGIIAHGMFSMAHLVKALKDWAGDPAALRSINVQFRAVVYMDETLLAKGRVA
                     SRDETSREVTLDVWAELERDGKTVQAIKNSQAVVRLD"
     gene            complement(2407..>2652)
                     /locus_tag="VG709_03530"
     CDS             complement(2407..>2652)
                     /locus_tag="VG709_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWC32176.1"
                     /translation="VVLDPELGLDYTRVVHGEQEYEWRRPIVVGDELRATPRIADIYA
                     RGPNEFVVIEAEILDASGETVCVARSTLLSRGTAGGS"
BASE COUNT          375 a          990 c          915 g          372 t
ORIGIN      
        1 gtgtcgtcgc gcggctcttc cacggtggtt cgggtgtagt cctccacggg cacctccggc
       61 tagccggcct ggccgccgca cacgaacagg accgccctgg agatgacgaa gtcgaagccg
      121 aacaccaggc tcgtcaggac gatgacggtg accagcacca cggtcgtgta ggcgaccatc
      181 tcgcgacggg tcggccacgc aaccttcttc agctcctggc ggacctcccg caggaactgc
      241 cgcggcccga cccgccgctt gcgctcgggc acggggcggc ggccggccgc gacgcgatcc
      301 tgcgtcgcct gctggcgctg gagcatccgc ttcgcctgtc ggttcatgtc ttctcctcgg
      361 cagggccgga gggacgcgaa cccccaaccg ccggttttgg agaccggtgc tctcccaatt
      421 gagctacggc cctttagcga tgggaccgct ggggcgtcga ccagtatacg ggctcgccca
      481 cccggccgaa aaggtgccct cggtgcgtga acgcgcacga tacctcccct agactgggga
      541 agcctcgcgc ggccccgaag cgaggtgcga tgcagcagat caaggagccg gcagcgatcg
      601 gatcgccgtc ccggcgggtc ccaccgcgtc gccggatccc gataccggat ccggaggtcg
      661 gggcgctcgc agaccgcatc gaccggctcg aggccgtcct ccgccaggcc gttcgcgcga
      721 acaaggaggt cctgcggacg ctcgaccccg cgcgttcgga gagcgacgcg gcggcgcggc
      781 ggcgcgagct cgccgcattc ggcgaccgcg tccgcgaggg gctcacgggc gttgcgcgcg
      841 tgatccgcgc agagcttcgc gcgacgcagg aacgtcagga cgacgtgcgt cgcgacgtcg
      901 cggagctccg cgaggcggtc gagtcgctct cgaggcagct cggcgaccgc cgggcgcccg
      961 agcgggcgcc ggtcacgcgg ctcgccgacg tgagactgcg gctctcgcgg atcaccgacg
     1021 agctggccgc cgacgtcgcc ccgtagctac gcggcccgag ccacgaggcc gcggcgggcg
     1081 atccgccacc agacgagcgc gatcgccgct cccccgacga gcgccccgcc gcgcgaggcg
     1141 gcggcgacgt ggacccgccg gtcctgcgcc gcgcgcgcag cggcggcgcg gatccgggcg
     1201 cgcggagaga gcaccgaggc cacgatccgc tccggcatcc cgacgggcgc tatctcgacg
     1261 tcgtcgcgga gcgacgcgag cgctcccatg agctcgcggt agcgcgcgag ctcgccggag
     1321 cactccgggc acgtcgcgag atgcacgtcg atgtcctcac gcactgcctt cctccacgta
     1381 ctcggggaga cgctcccgca cgtctcggtg cccctccggc gcgtcgccat agatcaggtc
     1441 cttgaggcgc ttgcgcgcgc ggtggaggcg caccttcacc gccccctccg agacgccgat
     1501 ctggtcgccg atctcctggc acgagagccc gtagacgtcc ttgagcacga cgaccgtccg
     1561 ctgcccgagc ggcagcgcct cgagcgcgcg cttcagctcg tcgcgctcga cgaggtcctc
     1621 ggtcggacgg acctcgggct cgggaccgcc gctgtcgtcg gcgacaaggt cgccgaaccg
     1681 tttccggcgg cggaggtgcg tcatggcgac gttggagacg atccggtaca tccaggtctc
     1741 gaaccgggcg tcgccccgga acccccggag cgagcgatgc gcccgcaggt atgcctcctg
     1801 cgccacgtcc tcggcctcct gccggtcccc caccaaccgg tacgcgagcg tgtagacccg
     1861 gcggtgggtc gtctccacca gctcctcgaa cgcccgcgga tcgccccgcc ggcacgcctc
     1921 gaggagctcg cggctcacca tgtcgtgacg ggttcgacgc cggtacgcgc cggcaggtta
     1981 cggacggtca atccagccgg acgacggcct gggagttctt gatggcctgg acggtcttgc
     2041 cgtcgcgctc gagctccgcc cacacgtcga gcgtaacctc ccggctcgtc tcgtcgcgcg
     2101 aggcgacgcg acccttcgcg agcagcgtct cgtccatgta gaccacggcg cggaactgca
     2161 cgttgatgct gcggagtgcc gccgggtcgc cggcccagtc cttgagcgcc ttcacgaggt
     2221 gcgccatcga gaacatcccg tgggcgatga tcccggggaa tcccacactg cgggcgaagt
     2281 cgtcgtcctg gtggaggggg ttctggtcgc cggacgcgtc ggcgtacgcc ttcacgtcct
     2341 ctcggcggat gaccttcggg accgagaaga cctcgtcgcc gaccgcgacg tcctcgaacc
     2401 tcgccatcac gaccctcccg ccgtgccgcg cgaaagcagc gtcgagcggg cgacgcagac
     2461 ggtctcgccc gacgcatcga ggatctccgc ctcgatcacg acgaactcgt tcggcccgcg
     2521 cgcgtagatg tccgcgatgc gcggcgtcgc gcggagctcg tccccgacga cgatcggccg
     2581 ccgccattcg tactcctgct cgccgtgcac gacccgcgtg tagtcgagcc cgagctcggg
     2641 atcgagcacg ac
//
LOCUS       DASZOB010000217         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_196728, whole genome shotgun sequence.
ACCESSION   DASZOB010000217 DASZOB010000000
VERSION     DASZOB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196728"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1117
                     /locus_tag="VG709_03535"
     CDS             47..1117
                     /locus_tag="VG709_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWC32177.1"
                     /translation="MSDGAVAGVLAAEHVAKRLTDRPVWIDGVHMKGEYLSDGAGAFE
                     KPYFRSFVGGGTGVFVAAHGWYQVASGMFDTCLVVAEEKMSSTQPHAQGAFITIFDNI
                     LERPLGPNLLWIFALEMNRYMTVHGLEKRDIAQVAVKNKRNAADHPAALLGEADITVE
                     DILNSETLAWPVQRLDVSPVSDGAVAVVLAAEHVAKRLTDRPVWIDGVGWHLDTAYWT
                     NRDLYYPRYVEYAARQAYDMAGIREPRKEIHIAEPYDPFDYKELHHLEGLLLFPPGKA
                     PEAMRDGVLNRDGDLPTCPSGGLLGVGNPIAAAGLMKIAELYWQLSGQAGARQVPGDP
                     RRGVAQAWGDLMQVGTVVVMGR"
     gene            1114..1734
                     /locus_tag="VG709_03540"
     CDS             1114..1734
                     /locus_tag="VG709_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWC32178.1"
                     /translation="MTASGNGGRKLPVVEPPPYATPGPPLSDQQFRDAVGAVDFAVDA
                     RYAWDAGVAISRFLDGLQEGRILARECRGPCRRVLVPPRMFCEEDFRPTDRWVDVQDT
                     GTVNTFSLCYVTWDMQPLEDPEIPSVIEIDGSDGGFLHKLGEVAPDDVSIGMEVEAVW
                     RPEAERTGSILDIRYFRPRDPSLRRGPKEPAGGGAEEGREDAGGSV"
     gene            1735..2208
                     /locus_tag="VG709_03545"
     CDS             1735..2208
                     /locus_tag="VG709_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWC32179.1"
                     /translation="MFEKLADPKEVRLVEGAVPIRHRYTPGVAGEVFFSALKERGELV
                     GSRCASCGITYVPARLFCERCFEELSGDVTVGPQGTLESFTVGFVGLEGEALDEPVPL
                     GLVRLDGADTVLMHFLVDFDEEHALGIGDRVEIALKPEGERTGSILDIRGFKPVD"
     gene            2330..3349
                     /locus_tag="VG709_03550"
     CDS             2330..3349
                     /locus_tag="VG709_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32180.1"
                     /translation="MSTHASLRLLAVAARDVDDVVAGKAGNGREPDRGIDDAVGVSVA
                     VELTRLPAGGWAATAERVRSFTTQRPFTLLISVSAELEDPSPDPKQALLALAGLVTGE
                     RGGRIVVLNASSVAAAGASMASGGSLELAIRRLNLVAVEVSHETGISVLDADRVVATA
                     SAGPKVGAALDYSPDVLRSLQAALVRILDDLGVGAGAVLEARVPFVRPAKRLRIERWL
                     SREGDRIEPGDRLCEIGLVGARRMTRPTNAVVLASISGRRTLRSLLSRERAGARARTG
                     TLSLVARDAGVLRRIMAARGAAIHPGDAIAVFTPEPDSAIEGVRPDAFRAMLQTDDPV
                     LSRML"
     gene            3406..4317
                     /locus_tag="VG709_03555"
     CDS             3406..4317
                     /locus_tag="VG709_03555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWC32181.1"
                     /translation="MVRLYDRLTPDFVVERGRDWVSYRGPRTVYLRNGSGPPRGIYLR
                     GACDAPAMFTLAPLVIDELRGSLCVHMGGTGVSDARSDLLLQTFTGVSPEFVEDVRSS
                     FRLPGAYFEPTLFEPEFSVRGLPESERFPKTVVVLSVLPDLTRTMYRHRATGYLVDPG
                     AGWLNDLAGALEDLSFVDWFRRNFESIGLMSADDFGESYRRLVPAIREATGAHVLVFN
                     ALEVEPSDATHDYSVRDFESATRRRRFNVALAELAEELGFRVIDVDRVLKEQGIERQV
                     DFSHFPVERMRAVAVEARRILRDLGVL"
     gene            4342..>5346
                     /locus_tag="VG709_03560"
     CDS             4342..>5346
                     /locus_tag="VG709_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC32182.1"
                     /translation="MDFDLSPEQQEFRKALRAFAEDVVAPRVEEMDKAEELPMDIVKQ
                     MGELGLFGLPFPEEYGGQGADLLTLCIAIEELARIDSSIAIPLEAAVGLGANPIYRHG
                     TEDQKQRWLVPMAKGEILGGFGLTEPGGGSDARSTLTTATLEGGEWVINGSKAFITNS
                     GTEISKVVTITAVTGERDGMKEISAIIVPTDTPGFRVGKHYRKLGWRASDTHEISFDD
                     CRVPEENLLGERGRGFAQFLETLDDGRIANAALGVGLTQGCLEESVKYAKEREAFGRP
                     IGSFQAIQFKVADMRVAAETARLAVYRAAWLRDNGRPFKAAASIAKVYATEAAVSAAR
                     D"
BASE COUNT          758 a         1862 c         1929 g          797 t
ORIGIN      
        1 ctcggagacg ctcgcggggc cggtgcagcg cctcgacgtc tcgccggtgt ccgacggggc
       61 cgtcgcgggc gtgctggcgg ccgagcacgt cgcgaagcgc ctgacggaca ggccggtgtg
      121 gatcgacggc gtccacatga agggcgagta cctctccgac ggcgccggcg cgttcgagaa
      181 gccgtacttc cgatcgttcg tcggcggcgg taccggcgtg ttcgtcgcgg cacacggctg
      241 gtaccaggtc gcctcgggga tgttcgacac gtgcctcgtc gtcgccgagg agaagatgtc
      301 ttctacgcag ccgcacgcgc agggcgcgtt catcacgatc ttcgacaaca tcctcgagcg
      361 gccgctcggc ccgaacctcc tgtggatctt cgcgctcgag atgaaccgct acatgaccgt
      421 ccacgggctc gagaagcgag acatcgcgca ggtcgccgtg aagaacaagc ggaacgccgc
      481 cgaccacccg gccgcgctgc tcggggaggc cgacatcacg gtcgaggaca tcctcaactc
      541 ggagacgctc gcgtggccgg tgcagcgcct cgacgtctcg ccggtgtccg acggggccgt
      601 cgcggtcgtg ctggcggccg agcacgtcgc caagcgcctg acggacaggc cggtgtggat
      661 cgacggcgtc ggttggcacc tcgacaccgc gtactggacc aaccgcgacc tctactaccc
      721 gcgttacgtc gagtacgccg cgcggcaggc gtacgacatg gccgggatcc gcgagccgcg
      781 caaggagatc cacatcgccg agccatacga cccgttcgac tacaaggagc tccatcacct
      841 cgaggggctg ctgctgttcc cgcccgggaa ggcgcccgag gccatgcgcg acggcgtgct
      901 gaaccgcgac ggcgacctcc ccacgtgccc ctcgggcggg ctcctcggcg tcggtaaccc
      961 gatcgccgcc gcgggcctca tgaagatcgc ggagctctac tggcagctat cgggacaggc
     1021 cggcgcccgt caggtgcccg gcgacccgcg ccgcggcgtc gcgcaggcgt ggggcgacct
     1081 catgcaggtc gggaccgtgg tggtgatggg gcgatgactg cgtcggggaa cggcggcagg
     1141 aagctgccag tcgtcgagcc gccgccgtac gcgacgcccg gccccccgct gtcggaccag
     1201 cagttccgcg acgccgtcgg cgcggtcgac ttcgccgtcg acgcgcggta cgcgtgggac
     1261 gccggcgtcg cgatctcgcg gttcctcgac gggttgcagg aaggccggat cctggcgcgc
     1321 gagtgccgcg gtccgtgccg gcgggtcctc gtgccgccgc gcatgttctg cgaggaggac
     1381 ttccgaccga ccgaccggtg ggtcgacgtt caggacaccg gcaccgtcaa cacgttctcg
     1441 ctctgctacg tgacgtggga catgcaaccg ctcgaggacc ccgagatccc gtcggtcatc
     1501 gagatcgacg gctccgacgg tggtttcctc cacaagctcg gcgaggtggc gcccgacgac
     1561 gtctccatcg gcatggaggt cgaagccgtc tggcgcccgg aagccgagcg gactggctcg
     1621 atcctggata tccgctactt ccgcccgcgc gacccgtcgc tgcgccgcgg gccgaaggag
     1681 ccggccgggg gcggcgccga ggaagggcgc gaggacgccg gagggagcgt ctaggtgttc
     1741 gagaagctcg cggacccgaa ggaggtccgg ctcgtcgagg gcgcggtgcc gatcaggcat
     1801 cggtacacgc ccggggtcgc cggcgaggtc ttcttcagcg cgctcaagga gcgcggcgag
     1861 ctcgtcggat cacggtgcgc atcctgcggc atcacgtacg tcccggcgcg gctcttctgc
     1921 gagcggtgct tcgaggagct ctccggcgac gtcaccgttg ggccgcaggg caccctcgag
     1981 tcgttcacgg tcggattcgt ggggctcgag ggcgaggcgc tcgacgagcc ggtcccgctg
     2041 gggctcgtcc gcctcgacgg cgccgacacc gtgttgatgc acttcctggt cgacttcgac
     2101 gaggagcacg cgctcgggat cggcgaccgc gtcgagatcg cgctcaagcc ggaaggcgag
     2161 cggacggggt cgatcctcga catccgcggc ttcaagccgg tcgactagcg ccaggtctcg
     2221 acgtctccgg gcggcaaagg gttccggcgg cactcctcaa ctatcatccg cgggaccgcg
     2281 tcctccgatg cggtcggacg atatggacga gctcaggacg gaggctcggg tgtctacgca
     2341 cgcgtcgctg cgcctgctcg cggtcgcggc gcgtgacgtc gatgacgtcg tcgcgggcaa
     2401 ggccggaaat gggcgcgagc ccgaccgcgg catcgacgac gccgtcggcg tctccgtcgc
     2461 ggtcgagctg acgaggctgc ccgcgggcgg atgggccgcg acggccgagc gcgtgcggtc
     2521 gttcacgacc cagcgaccgt tcacgctcct gatctccgtt tcggccgagc tcgaggatcc
     2581 gagtcccgac ccgaagcagg cgctgctcgc tctggccggc ctcgtgaccg gggaacgcgg
     2641 cggccggatc gtcgtgctga acgcctcgtc ggtcgcggcc gcgggagcgt ccatggcgtc
     2701 cggcggctcc ctcgagctcg cgatcaggcg cctgaacctc gttgccgtcg aggtctccca
     2761 cgagaccggc atctcggtcc tcgacgcgga ccgcgttgtc gcgacggcgt cggccgggcc
     2821 gaaggtgggc gccgcgctcg actactcgcc cgacgtgctc cggtcgctgc aggctgcgct
     2881 cgtgcggatc ctcgacgatc tcggggtcgg cgccggagcc gttctcgagg cccgcgtgcc
     2941 gttcgtgcgc cccgcgaagc ggctccgcat cgagcggtgg ctcagtcgcg agggggaccg
     3001 catcgagccg ggcgaccgcc tatgtgagat cggtctcgtc ggcgcgaggc ggatgacgcg
     3061 gcccacgaac gcggtcgtcc tcgcctccat ctccggccgc cggacgctcc gttccctgtt
     3121 gagccgcgag cgcgccggcg cgcgagcgcg caccgggacg ctctcgctcg tcgcgaggga
     3181 cgcgggcgtg ctgcgccgga tcatggccgc gcgcggagcg gcgatccacc ccggggacgc
     3241 gatcgcggtc ttcacccccg agccggactc cgcgatcgag ggcgtccgcc ccgacgcatt
     3301 ccgcgcgatg ttgcaaacgg acgacccggt gctctcgagg atgctgtgag tacccggccg
     3361 gggcgcggcc taccttcgtc gctccgctcg agggtcaggc gccgcatggt ccgcctctac
     3421 gaccgcctca cgccggactt cgtcgtcgag cgaggtcggg actgggtctc gtaccgcggc
     3481 ccgcggaccg tgtacctccg gaacgggagc ggcccacctc gcggcatcta cctgcgcggg
     3541 gcgtgtgacg cgcccgcgat gttcacgctt gcaccgctcg tgatcgacga gctccgcggc
     3601 tccctctgcg tccacatggg aggaaccggt gtctccgacg cgcggtcgga tctgctgctc
     3661 cagaccttca ccggcgtctc gccggagttc gtcgaggacg tgcgctcgag cttccgcctc
     3721 ccgggcgcgt acttcgagcc gacgctgttc gaaccggagt tctcggtacg gggtctcccg
     3781 gagagcgaac ggttcccgaa gacggtcgtc gtgctgagcg tcctgccgga cctcacgcgg
     3841 acgatgtacc gtcaccgcgc caccggctac ctcgtcgacc caggcgcagg gtggctgaac
     3901 gacctcgccg gggcgctcga ggacctttcg ttcgtggact ggttccggcg gaacttcgag
     3961 tcgatcggac tcatgagcgc cgacgacttc ggcgagagct accggcggtt ggtccccgcg
     4021 atccgggagg ccacgggcgc ccacgtcctc gtgttcaacg cgctcgaggt cgagccctcg
     4081 gacgccacgc acgactactc ggtgcgcgac ttcgagagcg cgacgcggcg gcggcggttc
     4141 aacgtcgcgc tcgccgagct cgcggaggag ctcggcttcc gcgtgatcga cgtcgaccgc
     4201 gtgctgaagg agcaggggat cgaacggcag gtcgacttct cgcactttcc cgtcgagcgg
     4261 atgcgggcgg tggccgtcga ggcgcgccgg atcctgcgcg atctcggcgt gctgtgacac
     4321 ctgccgggta ggctgtccgc gatggacttc gatctctcgc ccgaacagca ggagttccgc
     4381 aaggccctcc gggcgttcgc ggaagacgtc gtcgcgccgc gcgtcgagga gatggacaaa
     4441 gccgaggagc tcccgatgga catcgtcaag cagatggggg agctggggct cttcggtctg
     4501 ccgttccccg aggagtacgg gggccagggc gcggacctcc tcacgctctg catcgcgatc
     4561 gaggagctcg cccggatcga ctcgtcgatc gcgatcccgc tcgaggcggc cgtcgggctc
     4621 ggcgcgaacc cgatctaccg ccacggcacc gaggaccaga agcagcggtg gctcgtcccg
     4681 atggcgaagg gcgagatcct cggcggcttc ggtctcacgg agcccggcgg ggggtccgac
     4741 gcgcgctcga cgctcacgac ggcgacgctc gagggcggcg agtgggtgat caacggctcc
     4801 aaggcgttca tcacgaactc gggcaccgag atctcgaagg tcgtgacgat caccgcggtc
     4861 accggggagc gagacggcat gaaagagatc tccgcaatca tcgtcccgac cgacaccccg
     4921 gggttccgcg tcggcaagca ctaccggaag ctcgggtggc gcgcctccga cacccacgag
     4981 atctcgttcg acgactgtcg cgtgccggag gagaacctcc tcggcgagcg gggccgcggc
     5041 ttcgcgcagt tcctcgagac gctcgacgac ggccgcatcg cgaatgccgc gctcggcgtc
     5101 gggctcacgc agggctgcct cgaggagtcg gtgaagtacg cgaaggagcg cgaggccttc
     5161 ggcaggccga tcgggagctt ccaggcgatc caattcaagg tcgccgacat gcgcgtcgcg
     5221 gctgagacgg cccgcctcgc cgtgtaccgc gccgcctggc ttcgcgacaa cggccgcccg
     5281 ttcaaggcgg cggcctcgat cgcgaaggtc tacgcgaccg aggccgcggt gagcgcggcg
     5341 cgcgac
//
LOCUS       DASZOB010000218         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_24868, whole genome shotgun sequence.
ACCESSION   DASZOB010000218 DASZOB010000000
VERSION     DASZOB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24868"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VG709_03565"
     CDS             complement(<1..1301)
                     /locus_tag="VG709_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWC32183.1"
                     /translation="MAAAKTKKKPKKAPAKKPTASVGAAGRKVAEHLGPQLADAIGIG
                     LVVVAAISALGIWFDQAGPIGRFMWVAALGTFGIVGHAFPVVALWWGVILVRGTAEED
                     RGRMLVGLWMFLAGALGLISLVLGNPAPLSGLSRLVDAGGIVGSLAAWPLSKLLSVWG
                     AGIVCFALALLGALVFTATPLAQIARALGGRDREEAPEERPRRARRRQQEPPPAEIEA
                     VATLEDLVGEPEPEPEPEPVAAVPAPRPKRRGGGYELPPLDLLRRAPEAAKAGRSEEQ
                     TMSVLEQTLVNFGVSARVTGFTRGPTVTLFEVEVGEGTKVKKVLQLSDDIAYALATPD
                     VRIIAPIPGKSAIGVEVPNEVRDFVMLGDVLSSRRAKDEPHPLAVGLGKDVHGRPVLV
                     HLNRMPHVLIAGATGAGKSSLINAFVASVLMRATPDDVKLML"
     gene            complement(1304..2959)
                     /locus_tag="VG709_03570"
     CDS             complement(1304..2959)
                     /locus_tag="VG709_03570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWC32184.1"
                     /translation="MPAPSRLVFLGGLGEVGRNMACLEQDGSVLVLDAGLSFPTEDMP
                     GVDLVLPDFQYLRERKERIAGVVLTHGHEDHVGTLPYLLREMPLHVYATRLTLGLIRS
                     KIEEHGVEGSATLHEVAPGNAVRIGPFDVRFHRVTHSIPDGVAVAVDTTHGTLLHTGD
                     FRLDPTPIDGRLTDLQGLAEEARRGVHLLLSDSTNAEKPGTVPSERTVGPVLRDIFRD
                     ARRLIVAGCFSSHIHRIQQICDAARAVGRNVAFLGRSMNQSVEAARRLGYLEVPDDAI
                     VELDRVKELPREQLVVVSTGSQGEPMSALALMAAHEHKWVKLDEGDTVVLSSSVIPGN
                     ETSIHRVIDGLFRLGADVHHVGIEAVHVSGHAAAEELQLMMNLVRPRWFVPVHGERRH
                     LARHATLAEEVGIPRERILICEDGDVVEIGEEVRVADHVQAGRILVDGLGVGDVGDVV
                     LRDRRKLAGDGVVIAVVTADGQTGEVVGRPDIITRGFVHDETSEEFLEEARHRVLISL
                     KEVSDEEVGDPQILRQHVRRTLKKYFHEAIQRYPMIVPVIMEV"
     gene            complement(2943..3830)
                     /gene="dapA"
                     /locus_tag="VG709_03575"
     CDS             complement(2943..3830)
                     /gene="dapA"
                     /locus_tag="VG709_03575"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWC32185.1"
                     /translation="MAGKFGAVVTAMVTPFTEELELDVGRAQELARWLVDHGNDGVVV
                     AGSTGEGATLSDDEKVELWRAVVEAVGDRATVIANTGTNDTRQSIELTKHAEEAGADA
                     VLVVAPYYNKPPQRGLAEHFTRVAGATSLPVMLYNVPGRTAVRIDHATIVEVAGRVEN
                     VVAVKDSTGDVDGVARLVAEAPDGFEVYSGDDWATFSFLGLGARGVVSVAGHVVGERI
                     HDVVELVESGNVEAARKIDADLRDVYRALFITSNPIPVKKAMELIGQPAGPPRLPLVP
                     ATDEETAKIRKALEDAGAL"
BASE COUNT          570 a         1409 c         1346 g          555 t
ORIGIN      
        1 agcatcagct tcacgtcgtc gggcgtcgcg cgcatcagca ccgacgcgac gaacgcgttg
       61 atcagtgagg actttccggc gccggtcgcg ccggcgatca ggacgtgcgg catccggttc
      121 aggtggacga gcacgggacg cccgtggacg tccttgccga gcccgaccgc gagcgggtgc
      181 ggctcgtcct tcgcccgtcg cgacgagagg acgtcgccga gcatgacgaa atcgcggacc
      241 tcgttcggca cctcgacgcc gatcgcggac ttccccggga tcggcgcgat gatccgaacg
      301 tcgggcgtcg cgagcgcgta cgcgatgtcg tcggagagct ggagcacctt cttgaccttg
      361 gtgccctcgc cgacctcgac ctcgaagagc gtgacggtcg gcccgcgcgt gaaccccgtg
      421 acgcgcgcgg acacgccgaa gttgacgagc gtttgctcga ggaccgacat cgtctgctcc
      481 tcggagcgac ccgccttcgc ggcctcgggc gcgcgacgta gcaggtcgag cggcgggagc
      541 tcgtagccgc cgccccgccg cttcggtcga ggcgcgggaa cggccgcgac gggctcgggc
      601 tccggctcgg gctccggctc gcccacgagg tcctcgagcg tcgcgacggc ctcgatctcc
      661 gcgggcggcg gctcctgctg ccgccggcgt gcgcgccgcg gcctttcctc cggcgcctcc
      721 tcgcggtcgc gtcctcccag cgcccgcgcg atctgcgcga gcggcgtggc cgtgaagacg
      781 agcgcgccga gcaaggcgag cgcaaagcat acgatgccgg cgccccagac agacaggagc
      841 ttcgacagcg gccaggccgc gagcgacccg acgatcccac ccgcgtccac gagccgggag
      901 agcccggaga gcggggccgg gttcccgagc acgagcgaga tgagcccgag cgctcccgcg
      961 aggaacatcc acagcccgac gagcatgcgg ccgcggtcct cctcggcggt ccctcggacg
     1021 aggatcacgc cccaccagag cgcgacgacg gggaacgcgt ggccgacgat gccgaacgtg
     1081 ccgagcgccg ccacccacat gaaccgcccg atcggcccgg cctggtcgaa ccagatgccg
     1141 agcgccgaga tcgccgcgac gacgacgagc ccgatcccga tcgcgtcggc gagctgcgga
     1201 ccgaggtgct cggcgacctt acggcccgct gcgccgacgg acgcggtcgg cttcttcgcg
     1261 ggcgccttct tcggcttctt cttggtcttg gcggctgcca cgctcagacc tccatgatca
     1321 cgggcacgat cattggatac cgctggatcg cctcgtggaa gtacttcttc aaggttcgcc
     1381 ggacgtgctg ccggaggatc tgcgggtcgc cgacctcctc gtcggagacc tccttgagcg
     1441 agatcagcac acggtgccga gcctcctcga ggaactcctc cgaggtctcg tcgtgcacga
     1501 acccgcgcgt gatgatgtcg gggcgcccaa ccacctcgcc ggtctggccg tcggcggtca
     1561 cgaccgcgat cacgacgccg tcgccggcga gcttgcggcg atcgcggagc acgacgtctc
     1621 cgacgtcgcc gacgccgagc ccgtcgacca ggatgcgccc cgcctggacg tggtccgcga
     1681 cgcggacctc ctccccgatc tcgacgacgt cgccgtcctc gcagatcaag atgcgctcgc
     1741 gcgggatgcc gacctcctcg gcgagcgtcg cgtggcgcgc gaggtgccgc cgctcgccgt
     1801 gaaccgggac gaaccaacgc gggcgtacga gattcatcat gagctgcagc tcttccgccg
     1861 cggcgtgccc ggagacgtgc acggcctcga tgccgacgtg gtgcacgtcc gcgccgagac
     1921 ggaagagccc atcgatcacg cggtggatcg aggtctcgtt ccccgggatc accgacgacg
     1981 agagcacgac ggtgtcgccc tcgtctagct tgacccactt gtgctcgtgc gcggccatca
     2041 gcgcgagagc cgacatcggc tcgccctggc tccccgtcga gaccaccacg agctgctcac
     2101 gaggaagctc cttcacgcgg tcgagctcga cgatcgcgtc gtccgggacc tcgaggtacc
     2161 cgagccgccg cgccgcctcg accgactggt tcatgctccg cccgaggaac gcgacgttcc
     2221 gtccgacggc ccgcgcggcg tcgcagatct gctggatgcg gtggatgtgg ctcgagaagc
     2281 accccgcgac gatcagccgc cgcgcgtcac ggaagatgtc gcggaggacg gggccgaccg
     2341 tgcgctcgct cgggacggtc cccggcttct cggcgttggt cgagtcagac agcaggaggt
     2401 gaacgccacg gcgtgcctcc tccgcgagcc cctggaggtc ggtgagccgc ccgtcgatcg
     2461 gcgtcgggtc gaggcggaag tcgccggtgt gcagcagcgt cccgtgcgtc gtgtcgacag
     2521 ccaccgcgac gccgtcgggg atcgagtgcg tcacgcggtg gaagcggacg tcgaacgggc
     2581 cgatccgcac cgcgttgccc ggggccacct cgtggagcgt cgcgcttccc tccacgccgt
     2641 gctcctcgat cttcgaccgg atcaggccga gcgtgagcct ggtggcatac acgtgaagcg
     2701 gcatctcccg cagcaggtac gggagcgtcc ccacgtggtc ctcgtggccg tgggtgagga
     2761 ccacaccagc gatgcgctcc ttccgctcgc ggaggtactg gaagtccggg aggacgaggt
     2821 cgacgcccgg catgtcctcg gtcggaaacg acaggcccgc gtccagaacc agcactgagc
     2881 cgtcctgctc gaggcaggcc atgttgcgac cgacctcgcc gaggccgccg aggaagacga
     2941 gcctagaggg cgccggcatc ctccagcgcc ttccggatct tcgcggtctc ctcgtcggta
     3001 gcggggacga gcggcaagcg cggcggcccg gccggctgac cgatcagctc catcgccttc
     3061 ttcacgggga tcgggttgga cgtgatgaag agcgcccggt agacgtcgcg caggtcggcg
     3121 tcgatcttcc gcgccgcctc gacgttcccg gactcgacga gctcgacgac gtcgtggatc
     3181 cgctcgccga cgacgtggcc ggcgaccgag acgacgcctc gggcgccgag gccgaggaac
     3241 gagaacgtcg cccagtcgtc gccggagtac acctcgaacc cgtccggagc ctcggccacg
     3301 aggcgcgcaa caccgtcgac gtcgcccgtc gagtccttca ccgcgacgac gttctcgacg
     3361 cggccggcga cctcgacgat cgtggcgtgg tcgatgcgga cggcggtgcg ccccggcacg
     3421 ttgtagagca tcacggggag cgacgtggcg cccgcgacac gcgtgaagtg ctccgcgagc
     3481 ccacgctgcg gcggcttgtt gtagtacggc gcgacgacga ggacggcgtc ggcgccggct
     3541 tcctctgcat gcttcgtgag ctcgatcgac tggcgcgtgt cgttggtccc cgtgttcgcg
     3601 atcacggtcg cgcggtcacc gaccgcctcg acgacggcgc gccacagctc gaccttctcg
     3661 tcgtcggaga gcgtcgctcc ctcgccggtc gaaccggcga cgacgacccc gtcgttgccg
     3721 tggtccacga gccagcgcgc gagctcctgc gcccgcccga cgtcgagctc gagctcctcc
     3781 gtgaagggag tcaccatggc ggtgacgacc gctccgaact taccggccat cgtgtacctc
     3841 ctcaccggac ccgcgggcag cgcgggaccg ctccggccgg
//
LOCUS       DASZOB010000219         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_85370, whole genome shotgun sequence.
ACCESSION   DASZOB010000219 DASZOB010000000
VERSION     DASZOB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85370"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VG709_03580"
     CDS             <1..540
                     /locus_tag="VG709_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC32186.1"
                     /translation="DGRRMLAETRPDVLLLDELLPDGSGIDAAADLSEVVPGMRILIL
                     TSVEDDRVVAAAMQNGCSGVVTKDRPLEELLRAIRAVARGQSVFDAAALIGPARNSTG
                     PGSDLTPREHDVLRLLADGVSNAEIASRLGLSLNTVRNYIQTILGKLDAHSKLEAVAI
                     AARRGLVQVGRSSGGSAAG"
     gene            537..>1580
                     /locus_tag="VG709_03585"
     CDS             537..>1580
                     /locus_tag="VG709_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWC32187.1"
                     /translation="MTDPVAEVIDLLQHLIRNACVNDGTPGSGGESRNADVLERYLGS
                     GGFDLQRYEPVPGRASLLARIDGTDPGAPSLMLMGHTDVVPVNAERWREDPFGGELID
                     GEVWGRGAVDMLNLTASMAVAFRRLARKGFRPRGTLSFLAVADEEALGTHGAKWLTEE
                     RREDVATDYVVTEVGGFRMPLPTTGGPKLPLSVAEKGTYWCRIVVRGTAGHASMPLRT
                     DNALVKAAEVIRRLDTYRPPVRLHESWRGFVERLELPDEMRSELLDPAGVEQLGGSFP
                     DLGIARLVHASTHTTFAPTVVQGGVKTNVIPDRVELQVDIRTLPADTPDGIRAMLAEA
                     LGDLGAAVNVVADS"
BASE COUNT          224 a          561 c          581 g          214 t
ORIGIN      
        1 gacggccggc gcatgctcgc ggagacccgg ccggacgtcc tgctgctcga cgagctgctg
       61 cccgatggca gcgggatcga cgcggccgcc gacctcagcg aggtggttcc cggcatgcgg
      121 atcctgatcc tcacgtcggt cgaggacgac cgcgtcgtcg ccgccgccat gcagaacggc
      181 tgctcgggcg tcgtcacgaa ggatcgcccg ctggaggagc tcctccgggc gatccgcgcg
      241 gtcgcgcgtg ggcagagcgt cttcgacgcc gccgcgctga tcggacccgc tcgcaacagc
      301 accggtcccg gctcggacct gaccccccgc gagcacgacg tcctgcggtt gctcgccgac
      361 ggcgtctcga acgcggagat cgcgagccgg ctcgggctct cgctgaacac cgttcggaac
      421 tacatccaga cgatcctcgg caagctcgac gcgcactcga agctcgaggc cgtcgcgatc
      481 gcggcgcgca gggggctcgt gcaggtcggg cgctcgagcg gcggctcagc cgccggatga
      541 ccgacccggt cgccgaggtg atcgacctcc tccagcacct gatccgcaac gcgtgcgtca
      601 acgacgggac gccgggatcg ggcggggagt cgcgcaacgc cgacgtgctc gagcgctatc
      661 tcgggtcggg cggcttcgac ctgcaacgct acgagccggt ccccggccgg gcgagcctgc
      721 tggccaggat cgacggaacc gaccccggcg cgccctcgct gatgctcatg ggacacaccg
      781 acgtcgtgcc ggtgaacgcc gagcggtggc gcgaggaccc gttcggtggc gagctgatcg
      841 acggcgaggt ctggggtcgt ggcgccgtgg acatgctgaa cctcaccgcc tcgatggcgg
      901 tcgcgttccg gcggctggca cggaaggggt tccggccgcg cgggacgctg tcgttcctcg
      961 cggtggccga cgaggaggcg ctcgggaccc acggcgcgaa gtggctgacc gaagagcgac
     1021 gcgaggacgt cgcgaccgac tacgtcgtga cggaggtcgg cggcttccgg atgccgctcc
     1081 cgaccaccgg cgggccgaag ctgccgctga gcgtggccga gaaggggacg tactggtgcc
     1141 ggatcgtcgt ccgggggacg gcgggccatg cgtcgatgcc gctccgcacg gacaacgcgc
     1201 tcgtgaaggc cgccgaggtg atccgccgcc tcgacacgta ccgtccgccc gtccggttgc
     1261 acgagagctg gcgaggtttc gtcgagcgcc tcgagctgcc cgacgagatg cggtcggagt
     1321 tgctggaccc tgccggcgtc gagcagctcg gcggctcgtt cccggacctc ggcatcgcgc
     1381 gcctggtgca cgcgtcgacc cacaccacgt tcgcgcccac ggtcgtccaa ggtggcgtca
     1441 agacgaacgt gatccccgac cgggtcgagc tccaggtgga catccggacg ctgcccgccg
     1501 acaccccgga cggcatccgg gcgatgctcg cggaggcgct gggcgatctc ggcgccgcgg
     1561 tcaacgtcgt cgccgacagc
//
LOCUS       DASZOB010000220         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_65506, whole genome shotgun sequence.
ACCESSION   DASZOB010000220 DASZOB010000000
VERSION     DASZOB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65506"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VG709_03590"
     CDS             complement(<1..746)
                     /locus_tag="VG709_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32188.1"
                     /translation="MSGREQRRARLAWSLWGLVVLLTSIAIVFLVLAIEVPLPEDAFG
                     FRGFGAVWALVYATFGALVASRQPRNAIGWLFLGVGITSAVQELCQEYAMYSLLLRTG
                     DLPAGAVAAWVTAWIWVPMVWAGSAMVFLLFPDGRLPSPAWRPVAFVSTASMAIATLG
                     FAVIPGEVEGFIAVRNPFGVQLPRAVVEAVSFGALMLFVATVVASVVALLRRFRRATG
                     DERQQVKWVVAAASLLSFVILSGLTAYAVST"
     gene            complement(743..>1932)
                     /locus_tag="VG709_03595"
     CDS             complement(743..>1932)
                     /locus_tag="VG709_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWC32189.1"
                     /translation="VGVGALVGRRGGLVLPGVAAAAVALAFQPARQRARHLANRLVYG
                     KRATPYEVLSDFSDRLGEAYAFEDVLPRMADLVREGTGSVSATVWVRVGGEMRPAATS
                     PARAGVDPARVAVDDDALPELPGERALAVRHRGDLLGALTVSKSRGDPITPAEEKLLS
                     DLAAQAGLVLRNVRLTEELKANLEELRASRQRIVAAQDEERRRIERNIHDGAQQQLVA
                     MRVRLRLAQTLAAKDPAKAERMLEQLQGEMADALENLRDLARGIYPPLLADAGLAAAV
                     ESQARKSPVPVAVEADGIGRYGRDVEAAVYFCVLEAVQNASKYAGEAHVRVRLFQRNG
                     ELAFGVEDDGPGFDVATTPRGSGLQNMADRIEALGGTLEIRSAPEQGTVVAGRVPAEG
                     IAT"
BASE COUNT          329 a          720 c          636 g          247 t
ORIGIN      
        1 gtcgagaccg cgtacgcggt cagccccgac aggatcacaa aactgaggag agacgccgcg
       61 gcgacgaccc acttcacctg ctgtcgctca tcgccggtcg cacggcggaa gcggcgcagg
      121 agcgcgacca cggatgcgac aaccgtggcc acgaacagca tgagcgcgcc gaacgacacc
      181 gcctcgacga ccgcgcgagg aagctgtaca ccgaacgggt tccggacggc gatgaaccct
      241 tccacctcgc ccggtatgac ggcgaacccc agagtcgcga tcgccatcga ggcggtcgac
      301 acgaatgcga ccggccgcca cgcgggggac ggaagccgtc cgtcggggaa cagcaggaac
      361 accatcgcgg atcccgccca gaccatcggc acccagatcc acgcggtcac ccacgccgcc
      421 acagccccgg ccggcagatc gcccgtccgc agcaggagcg agtacatggc gtactcctgg
      481 cacagttcct ggaccgccga cgtgatcccg acaccgagga acagccagcc gatcgcgtta
      541 cggggttgcc gcgatgcgac gagcgccccg aacgtcgcgt acacgagcgc ccagacggca
      601 ccgaacccgc ggaagccgaa cgcgtcctcc ggcaggggga cctctatcgc gaggacgagg
      661 aacacgatgg cgatggacgt gagaaggacc acgagccccc agagggacca tgccagccgc
      721 gcgcggcgct gctctcgccc gctcatgtcg ctattccttc ggcgggaacg cgacccgcga
      781 caacggtccc ctgctccggc gccgaccgga tctccagcgt tccgccgagc gcctcgatgc
      841 ggtcggccat gttctgcaac cccgagccgc gaggcgtcgt cgcgacatca aagcctggac
      901 cgtcgtcctc gaccccgaac gcgagctcgc cgttccgttg gaacagcctg acgcgcacat
      961 gcgcctcccc ggcgtacttc gaggcgttct ggacggcctc gaggacgcag aagtacacgg
     1021 ccgcttcgac gtcgcggccg taccggccga tgccgtcggc ctcgacggcg acgggtaccg
     1081 gcgatttgcg cgcttgcgac tcgaccgcgg ccgcgagccc ggcgtcggcc agcagcggcg
     1141 ggtagatccc tcgcgcgagg tcgcgcaggt tctcaagcgc atccgccatc tcgccctgaa
     1201 gctgctcgag catccgttcc gccttggccg ggtccttcgc cgctagcgtc tgcgccagcc
     1261 gaagccgcac ccgcatcgcg acgagctgct gctgcgcgcc gtcgtggatg ttccgctcga
     1321 tgcgtcgccg ctcctcgtcc tgcgccgcga cgatccgttg ccgtgatgct cgaagctcct
     1381 cgagattcgc cttgagctcc tccgtcagcc gcacgttgcg caagacgagt ccggcctgtg
     1441 ccgcaagatc ggagaggagt ttctcctccg ctggtgtgat cgggtcgcct cgggacttag
     1501 agacggtcag ggcaccgagg aggtcgccgc ggtgtcgaac cgcgagcgcc cgctcgcccg
     1561 gaagctccgg cagcgcgtcg tcgtccaccg caacacgcgc ggggtccacg ccggcgcgcg
     1621 ccggggatgt cgcggccgga cgcatctctc ccccgacgcg aacccacacc gtcgcgctaa
     1681 cggagccggt tccctcgcgg acgagatccg ccatgcgagg gaggacgtct tcgaacgcat
     1741 acgcctcgcc gaggcggtcg gagaagtccg agaggacctc gtagggcgtg gcgcgcttcc
     1801 cgtagacgag ccggttcgcg agatggcgtg cacgctgccg cgccggctgg aacgccagcg
     1861 cgacggcggc cgcagcgacg cccggcagaa cgagaccccc acgtcgaccg acgagcgccc
     1921 ccacgcccac ga
//
LOCUS       DASZOB010000221         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_114812, whole genome shotgun sequence.
ACCESSION   DASZOB010000221 DASZOB010000000
VERSION     DASZOB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114812"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VG709_03600"
     CDS             <1..499
                     /locus_tag="VG709_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32190.1"
                     /translation="RRAGGVELFPLPAKLHRSCREAAEHAPILCPSVFPRRAVEPRSP
                     PVAQQLPFDEGLYGLELGYSAPYENAPRRNRPDRFLHFVVIAQRGPAAYAVGAEPLER
                     ATIGERHGRLSFVTEFSVHYDHVVFEWREDGVRYQASLHAWDDRRATTALLRALVATL
                     RPPPP"
     gene            537..1499
                     /gene="corA"
                     /locus_tag="VG709_03605"
     CDS             537..1499
                     /gene="corA"
                     /locus_tag="VG709_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWC32191.1"
                     /translation="MLTVRLYQAGRVDERRMPPERVGELLEEPDTIVWLDLVDPTRRE
                     IETIQQQFDLHELTVEDVQHKGQRPKFELYPNYYFVVLYGIAIDGDDLRTHEVHAFVA
                     PRFLATIRYGPALPLEPMLERCRQHAGLAKEGAGFLLYALLDYVVDDYFTVAERIEDR
                     GEAIEDAVFEDVPGHEVQQELFTLRKQLVTFRRLVAPMREVVNAVSDDTKLVTEQLRF
                     YYRDVTDHVLRVLDYVDNMRELLTTAQEVRIAQVGNRLNTVMKKVTSWAAILLVPTLI
                     AGIYGMNFRYMPELDWRFGYPFSLGLMALAGFVLYRLFRRQDWL"
     gene            complement(1496..2053)
                     /locus_tag="VG709_03610"
     CDS             complement(1496..2053)
                     /locus_tag="VG709_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWC32192.1"
                     /translation="MRRRTRSAKVARAAAVAALISLLALSADAHPLRWFWRYDPIYFR
                     ADHGWPDHYRLRRTHRAWHDAHPQTPLQKHREFHHELVHDHRRMHYHRQLRRQTGQAS
                     WYRANGETGACGRELRGMYAAHRRWPCGSLVSVRRGNKHIFVRIRDRGPYVDGRVIDL
                     SRRAFRKLADPSAGVLNVTIYRLED"
     gene            complement(2078..2638)
                     /locus_tag="VG709_03615"
     CDS             complement(2078..2638)
                     /locus_tag="VG709_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWC32193.1"
                     /translation="MNRRPNKIFVRGLMAAFLTSLLILTTQVPSGGFSGAGGTRMRYG
                     TVNGGRILPIAQAREKARRMVRRQTRRMMSGGRVRVTLRTFRKYTFWGEATTYSTATN
                     GTATACGRPLRDGDLTAAHRTLPCGSKVLVQNPANGNRVKVTITDRGPYGDSGRVIDL
                     SSAAWRRVTNRPPGVAHVHAIVLRRG"
BASE COUNT          395 a          939 c          935 g          462 t
ORIGIN      
        1 gcgccgagcc ggcggcgtcg agctcttccc gcttccggcg aagctccacc gatcctgccg
       61 cgaggccgcg gagcatgcgc cgatcctgtg tccctccgtc tttccgcgtc gagcggtcga
      121 gcctcgatcg ccgcccgtcg cgcagcagct tccctttgat gaggggctgt acggcctgga
      181 gctcggctat tcggccccgt acgagaacgc acctcgccgc aaccgccccg accgcttcct
      241 ccacttcgtc gtgatcgcgc agcgggggcc ggccgcgtac gccgtcggtg cggagccgct
      301 cgagcgtgcg acgatcggtg agcggcacgg gcggctctcg ttcgtcaccg agttttcggt
      361 ccactacgac catgtcgtgt tcgagtggcg ggaggacggc gtccggtacc aggcgagcct
      421 gcacgcctgg gatgatcgac gcgcgaccac ggcgctcctc cgagcgctgg tcgcgactct
      481 ccgaccgccg ccgccgtagc acggaacccg tcgaaggcgt ggggtacgct cgcgcggtgc
      541 tgaccgtgcg gctgtaccag gcgggccgtg tcgacgagcg gcggatgccg ccggagcgcg
      601 tcggcgagct cctcgaggaa cccgacacga tcgtctggct cgacctcgtc gatccgacgc
      661 gccgcgagat cgagacgatc cagcagcagt tcgacctcca cgagctgacc gtggaggacg
      721 tccagcacaa gggacagcgc ccgaagttcg agctctatcc gaactactac ttcgtcgtcc
      781 tgtacgggat cgcgatcgac ggagacgacc tgcgcacgca cgaggtccac gcgttcgtgg
      841 ctccgcggtt cctcgcgacg atccggtacg gccccgcgct cccgctcgag ccgatgctcg
      901 agcgctgccg gcagcacgcg ggcctcgcca aggagggcgc cgggttcctg ctgtacgcgc
      961 tcctcgacta cgtcgtcgac gactacttca cggtcgcgga gcggatcgag gaccgaggcg
     1021 aggcgatcga agacgcggtg ttcgaggacg tgcccggaca cgaggtccag caggagctct
     1081 tcacgctccg gaagcagctc gtgacgttcc ggcgtctcgt cgctccgatg cgcgaggtcg
     1141 tgaacgccgt gagcgacgac acgaagctcg tcaccgagca gctacgcttc tactaccgcg
     1201 acgtgacgga ccacgtgctc cgcgtcctcg actacgtcga caacatgcga gagctgctga
     1261 cgacagccca ggaggtgcgg atcgcgcagg tcgggaaccg gctgaacacg gtgatgaaga
     1321 aggtcacgtc gtgggcggcg atcctgctcg ttccgacgct gatcgcgggg atctacggga
     1381 tgaacttccg gtacatgccg gagctcgact ggcggttcgg gtacccgttc tcgctcggtc
     1441 tcatggcgct cgccgggttc gtgctctacc gcctgttcag gcggcaggac tggctctagt
     1501 cctcgagccg gtagatcgtc acgttgagga cgcccgccga ggggtccgcg agcttgcgaa
     1561 aggcccgtct cgacaggtcg atgacgcgcc cgtcgacgta ggggccgcgg tcgcggatcc
     1621 ggacgaagat gtgcttgttc cctcgtcgca cggatacgag cgagccgcac ggccaccgac
     1681 ggtgcgccgc atacatcccg cggagctcgc gcccgcatgc gccggtctca ccgttcgcgc
     1741 gataccagct cgcctggccg gtctggcgtc ggagctgccg gtggtaatgc atccggcggt
     1801 ggtcgtggac gagttcgtgg tggaactccc tgtgcttttg gagaggggtc tggggatgcg
     1861 cgtcgtgcca cgctcggtgc gttcgacgga gccggtagtg atccggccac ccgtggtcgg
     1921 cccggaagta gatcgggtcg tatcgccaaa accaccggag cgggtgtgcg tccgcggaga
     1981 gggcgagcag cgagatcagc gccgcgacgg ccgccgcgcg agccaccttg gcggaccgtg
     2041 tgcgtcgtct caccgctcgc cctcccctcg cgtgtcgcta tccgcgtcga agcacgatcg
     2101 cgtgcacgtg cgccacgccc ggcgggcggt tcgtcacccg ccgccatgcg gcgctcgaca
     2161 gatcgatgac gcgccccgaa tcgccgtacg gcccgcgatc ggtgatcgtg accttcaccc
     2221 ggttcccgtt cgccgggttc tggacgagaa ccttcgagcc gcacgggagc gtacggtggg
     2281 cggccgtgag atcaccgtca cggagcggac gcccgcacgc cgtcgcggtg ccgttggtcg
     2341 ccgtgctgta ggtcgtcgcc tcgccccaga acgtgtactt gcggaacgtg cggagcgtga
     2401 cgcggactcg ccccccgctc atcatccgcc gcgtctgtcg ccggaccatc ctgcgggcct
     2461 tctctcgtgc ctgggcgatc gggaggatcc gcccaccgtt gaccgtaccg taccgcatcc
     2521 tcgtgcctcc ggctccgctg aagcctcccg aaggaacctg cgtcgtgagg atgagcagtg
     2581 acgtgagaaa cgcggccatg aggccgcgca cgaagatctt gttgggtcgt cgattcatgg
     2641 attcgctctc acccccgtgg ctcagccggg gttatcggcc cccggcgtcg gcctggtagg
     2701 tcgtgggtgt tcgctccacc tcctcttagg g
//
LOCUS       DASZOB010000222         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173954, whole genome shotgun sequence.
ACCESSION   DASZOB010000222 DASZOB010000000
VERSION     DASZOB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173954"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VG709_03620"
     CDS             complement(<1..814)
                     /locus_tag="VG709_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWC32194.1"
                     /translation="MRIAQVAPPWVAVPPTGYGGIEWIVSLLADGLVDRGHDVTLYAT
                     GDSTTKARLEYAFAEAPGSAAINDAWYDVVHQQRVVADAARFDVLHQHTAYAAMVAAM
                     AVDVPVVHTLHNAATSKLRRIYEGVAGSVRFVAISEAQRRGVPELGDVDVVYNGIDLE
                     GYPYRESKEDFLLFLGRTSPDKGPRRAVEAARAAGMPLVMAVKRAEPVEVEHWDRDVE
                     PILGDDVTVIGEIPLEEKVDLLARARAVLFPIDWDEPFGLVMTEAMACGTPVV"
     gene            889..1932
                     /locus_tag="VG709_03625"
     CDS             889..1932
                     /locus_tag="VG709_03625"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HWC32195.1"
                     /translation="MRRGFLGGLTVLLVLVGGPALACGGLVAPNGAVTLDKTTTLAAY
                     VDGVEHYMTAFTFRSAAKEFGSIVPLPGVPTKVDKGGRWTLQRLVEEVQPPVDADLAL
                     AAGEAQAARGVEVIQRHRVGALDVLVVKGGGTEVSEWAREHGFDLPGDASEVLDFYSV
                     RSPIFAAIRFDSERAAREGFDAGDSAPVHFMIPTDNPWVPLRILALGTGASERVKADV
                     FLLTEEEPSLLPRAGQPFSFNRKLPLSLERSEPASRDLLRDLRSDRGMRWLPRSGMWF
                     SYLDLDVPAGELTWDLAVDGSGAGRPSLVDAGLLEPRIPDANAPVAWAVIAALGALTA
                     LAIVEERRLRARA"
BASE COUNT          274 a          706 c          718 g          289 t
ORIGIN      
        1 cgacgaccgg agtgccgcac gccatcgcct cggtcatcac gagcccgaac ggctcgtccc
       61 agtcgatcgg gaagagcacc gcgcgcgcac gcgccaggag atccaccttc tcttcgagcg
      121 ggatctcgcc gatcacggtg acgtcgtcgc cgaggatcgg ctcgacgtct cggtcccagt
      181 gctcgacctc gaccggctcc gcgcgcttca cggccatgac gagcggcatg ccggccgcgc
      241 gcgcggcctc cacggcccgc cgcggtccct tgtcggggct ggtccggccg aggaacagca
      301 ggaagtcctc cttcgactcg cggtacggat acccctcgag gtcgatgccg ttgtagacga
      361 cgtcgacgtc tccaagctcc ggcacgcccc gccgctgcgc ctcgctgatc gcgacgaagc
      421 ggacgctccc cgcgacgccc tcgtagatcc ggcggagctt cgatgtagcg gcgttatgga
      481 gcgtgtggac gacgggaacg tcgaccgcca tcgcggccac catcgccgcg taggccgtgt
      541 gctggtggag gacgtcgaac cgcgccgcgt cggcgacgac gcgctgctgg tgaacgacgt
      601 cgtaccaggc gtcgttgatc gccgccgatc ccggcgcctc ggcgaacgcg tactcgagcc
      661 tcgccttcgt cgtcgagtcg ccggtggcgt agagcgtgac gtcgtggcct cgatcgacga
      721 gcccgtccgc gaggagagac acgatccact cgatcccccc gtagcccgtc ggcgggacgg
      781 cgacccacgg cggcgcgacc tgcgcgatgc gcacggcgcg taatgctacg gcgcccggga
      841 acccaccggc ccctcgcgcc gtccaagaca cgcgaggagg tggagaggat gcgaagggga
      901 ttcctcgggg gactcacggt gctgctggtg ctggtcggag ggccggcgct cgcgtgcggc
      961 gggctcgtgg cgccgaacgg agcggtcacg ctggacaaga cgacgacgct cgcggcgtac
     1021 gtcgacggcg tcgagcacta catgacggcg ttcacgttcc gttcggccgc gaaggagttc
     1081 ggctcgatcg tcccgctgcc gggcgtgccg acgaaggtcg acaagggcgg gcggtggacg
     1141 ctccagcggc tcgtcgagga ggtccagccg ccggtcgacg cggacctcgc gctcgccgcc
     1201 ggcgaagcgc aggcggcgcg gggcgtcgag gtgatccagc ggcaccgcgt cggcgccctc
     1261 gacgtgctcg tcgtcaaggg cggcggcaca gaggtctccg agtgggcccg cgagcatggc
     1321 ttcgacctgc cgggcgacgc gtcggaggtc ctcgacttct actcggtgcg gagcccgatc
     1381 ttcgcggcga tccgcttcga ttccgagcgc gccgcgcgcg agggtttcga cgcgggagac
     1441 agcgcccccg tccacttcat gatcccgacc gacaacccgt gggtaccgct gcggatcctc
     1501 gcgctcggca cgggcgcgtc ggagcgagtg aaggcggacg tcttcctcct taccgaggag
     1561 gagccgtcgc tgctcccgcg cgccggacag ccgttctcgt tcaaccgcaa gctcccgctc
     1621 tcgctcgagc gcagcgagcc ggcctcgcgc gacctcttgc gtgacctccg gagcgaccgt
     1681 gggatgcgct ggctgccgag gtcgggcatg tggttctcct acctcgacct cgacgtgccg
     1741 gccggagagc tcacatggga cctcgccgtc gacgggagcg gcgcggggcg gccttcactg
     1801 gtcgacgcgg ggctgctgga gccgcgcatc cccgacgcga acgcccccgt tgcatgggcc
     1861 gtcatcgcgg cgctcggcgc gctcaccgcg ctcgcgatcg tcgaagagcg gcgtctgcgc
     1921 gcgcgggcgt gaggtaccat cggtgcgcgg gacgtagcgc agcttggtag cgcgcagcgt
     1981 tcgggac
//
LOCUS       DASZOB010000223         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_85449, whole genome shotgun sequence.
ACCESSION   DASZOB010000223 DASZOB010000000
VERSION     DASZOB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85449"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VG709_03630"
     CDS             <1..298
                     /locus_tag="VG709_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWC32196.1"
                     /translation="LSAGRPLLYRYAPETDGMEGREGAFLACSFWLVEALAALGRTDA
                     AAEVFEAMCERSNDLGLYAEEMDPATGEQLGNFPQALTHGALVCAALALRGATG"
     gene            complement(288..761)
                     /locus_tag="VG709_03635"
     CDS             complement(288..761)
                     /locus_tag="VG709_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32197.1"
                     /translation="MDWAGWATFGFVATVVLTGVMVAAQLAGLSRMDLPMMLGTMFVA
                     DPDRARVVGFVMHLANGQGFALLYAAAFTLLGTATWWLGAIFGLVHGAAALTVIVPLM
                     PGLHPRMASERQGLELGQLLEPPGLFALNYGRETPLMTLVAHVAYGTILGSFLTP"
     gene            815..1153
                     /locus_tag="VG709_03640"
     CDS             815..1153
                     /locus_tag="VG709_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWC32198.1"
                     /translation="MNKASLVSEVARRTNLSRSEAARVVEAVLDTVRDTVARGQRVSL
                     AGFGTFERRRRNPRTGRNPHTREAVAIPARNVPAFRPGSAFRTAVAAPQRKRTTRTRA
                     AAPRTSRRGR"
     gene            complement(1150..1746)
                     /locus_tag="VG709_03645"
     CDS             complement(1150..1746)
                     /locus_tag="VG709_03645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWC32199.1"
                     /translation="MGFRRRPPPRRCARGVPRGGRLPWRRLPEAAPVVRVHAERPARR
                     PVRPDRRRGRRTVVFHVGPEPSPLDSRWSEPRLLADVARRYPDVRFLAAHCGNEAWRE
                     TLDAVRDLNNVSIDLSGWQVRFAQDAGRFYEDVSEVLDALGAERVMWGTDPPYYRPLV
                     PDSRWLAAFTEAPDGTFATEEVDMITGANAVSFFGLEP"
     gene            complement(1661..2062)
                     /locus_tag="VG709_03650"
     CDS             complement(1661..2062)
                     /locus_tag="VG709_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32200.1"
                     /translation="MRVDAHAHVTRDGWIGERWWNAYAEEAARRVGLPAETVHQIVVP
                     AMFDDDEGSLQLGAMDEARIDIAVAYPFDWTLAERSVPPASGGASRTSGTRGSRNGTP
                     VGCAGVSAPTPATTVRSRRSARRSPPVAPSA"
     gene            complement(2064..2270)
                     /locus_tag="VG709_03655"
     CDS             complement(2064..2270)
                     /locus_tag="VG709_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWC32201.1"
                     /translation="MPQGTIKTYDREQRTGVLLTDDQQEVGIDVTSMEGSGIRYLRIG
                     QRVKFDVADEGGKKVARTLRIVTF"
     gene            complement(2279..2923)
                     /gene="cofC"
                     /locus_tag="VG709_03660"
     CDS             complement(2279..2923)
                     /gene="cofC"
                     /locus_tag="VG709_03660"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWC32202.1"
                     /translation="MRIIAVPVKSLERAKGRLIPVLSPLERAALTLAMLEDVLDACVD
                     IPGWHTWVVSPDEAVLEVAARRRTRAVPERDPGLNAAVRQVEEDAAGADALAVVLGDL
                     PLLHTDALARVLRTLGAVVAAPSDSDGGTNVLLRRPTAVIAPRFGTDSFRKHQDAAET
                     RGLPFAAVRDPALAFDLDRPDDVRTLLARGRRSRTVAACHEMRVTDRLTVASER"
     gene            complement(2966..3355)
                     /locus_tag="VG709_03665"
     CDS             complement(2966..3355)
                     /locus_tag="VG709_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32203.1"
                     /translation="MKRTRRLILSLLLVIGVVAPALPASAHFTQFEVAGITYHFGLGT
                     TFAGIRFTCADGEKYQIRTKVEIKPDYEGIGRASGRCTGTEETIWVESRKQSGELRAR
                     PARIVARGLTFLGGVQHGDAVKDVERF"
     gene            3483..4187
                     /locus_tag="VG709_03670"
     CDS             3483..4187
                     /locus_tag="VG709_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWC32204.1"
                     /translation="MRRPEPWMRFATSVLVPPIWVWFDWRFEGTEHLPPEGPVLVAAN
                     HISYFDPLAHGYLAVKAGRRPRFLTKIELYGNPFMRSLLQGARQIPVRRGSGEHGPVE
                     ATIKALRADELVIVYPESTITRNPDFSPMQGKTGVARVTLKTGVPVLPMAVWGSAPVW
                     QKGGRRSLKYGRPIWVKAGPPLDFTRYEDGADDPAVLRKVTDEVMAQLSLLVEDMRAR
                     YPKRWGELPPPAEGGS"
     gene            4184..>4455
                     /locus_tag="VG709_03675"
     CDS             4184..>4455
                     /locus_tag="VG709_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWC32205.1"
                     /translation="MNGDPRVAVIGAGSWGTAFATIPAEKGIETVLWARRAELADEIN
                     ARHVNPDYLSEFELPPSLSATSDVGKAVDGAGAVVMAVPSHAFRGV"
BASE COUNT          667 a         1590 c         1590 g          608 t
ORIGIN      
        1 cctgtcggcc gggcgcccgc tcctctaccg ctacgcgccc gagacagacg gcatggaggg
       61 acgagagggc gcgttcctcg cctgttcgtt ctggctcgtc gaggcgctgg cagcgctcgg
      121 acgaacggac gcggctgccg aggtgttcga ggcgatgtgc gagcggtcga acgacctggg
      181 gctctatgcc gaggagatgg acccggcgac gggggagcag ctcgggaact tcccgcaggc
      241 gctcacgcac ggcgccctcg tgtgcgccgc gctcgccctg cgcggcgcta cggggtgagg
      301 aacgatccga ggatcgtgcc gtacgccacg tgggccacga gcgtcatgag cggggtctcg
      361 cgcccgtagt tcagcgcgaa gagccccgga ggctcgagga gctggccgag ctcgagaccc
      421 tggcgctccg acgccatccg cgggtgcagg cccggcatca gcggcacgat gaccgtgagg
      481 gccgccgcgc cgtggacgag accgaagatc gcgccgagcc accacgtcgc ggtcccgagg
      541 agcgtgaacg ccgcggcgta gagcagcgcg aagccctgcc cgttggcgag gtgcatgacg
      601 aacccgacga cgcgggcccg atcgggatcg gcgacgaaca tcgtcccgag catcatcggg
      661 aggtccatcc gagagagccc tgcgagctgc gccgcaacca tcacgcccgt cagcacgacc
      721 gtcgcgacga acccgaacgt cgcccatccg gcccagtcca cgacccgaag ggtagcgatt
      781 gacgcgcggt tcgcggctcc atatcgtccc gcgcgtgaac aaggcgtcgc tcgtctccga
      841 ggtcgcgcga cggacgaacc tgtccaggag cgaggcggcg cgtgtcgtgg aagcggtcct
      901 cgacaccgtg cgcgacacgg tcgcgcgcgg gcagcgcgtg tcgctcgccg gcttcggcac
      961 gttcgagcga cgccggcgga accccaggac cggccgcaac ccgcacacgc gcgaggccgt
     1021 cgcgatcccg gcccggaacg tcccggcctt ccgtcccggt tccgcgttcc gcaccgcggt
     1081 cgcggcgccg cagcggaagc ggacgacgcg cacgcgcgcg gccgcgccga ggacctcgcg
     1141 tcgaggccgc tagggctcga gcccgaagaa cgacacggcg ttcgccccgg tgatcatgtc
     1201 gacctcttcg gtcgcgaacg tcccgtccgg cgcctcggtg aacgccgcga gccatcggct
     1261 gtccgggacg agcggacggt agtacggagg atcagtgccc cacatgacgc gctccgcgcc
     1321 gagcgcatcg agcacctcgc tgacgtcctc gtagaagcgg ccggcgtcct gcgcgaaccg
     1381 cacctgccat ccggacaggt cgatcgagac gttgttgagg tcgcggacgg cgtcgagcgt
     1441 ctcccgccac gcctcgttcc cgcagtgcgc agccaggaag cgaacgtcgg ggtaccgacg
     1501 cgcgacgtcc gcgaggagcc gcggctcaga ccagcgagag tccagcgggg acggctccgg
     1561 cccgacgtgg aagacgacgg tacgccgtcc tcgccggcgg tccggacgaa cgggtcgacg
     1621 agcgggtcgt tcagcatgaa cccggacgac gggtgcagct tcaggcagac ggcgccacgg
     1681 gaggcgaccg cctcgcggaa cgcctcgagc gcaccgtcgt ggcgggggtc ggcgccgaaa
     1741 ccccagcgca cccgaccggg gttccgttcc gcgaaccgcg cgtaccactc gttctgctcg
     1801 cgccacccga cgcgggcggg accgagcgct ccgcgagcgt ccagtcgaag ggataggcga
     1861 ccgcgatgtc gatccgggcc tcgtccatgg ccccgagctg cagcgacccc tcgtcgtcgt
     1921 cgaacatagc cggcacgacg atctggtgga cggtctcggc cggaaggccc acgcgacgcg
     1981 cggcctcctc cgcgtaggcg ttccaccatc gttcgccgat ccagccgtcg cgggtgacgt
     2041 gcgcgtgcgc gtcgacgcgc acctcagaac gtcacgatgc ggagggtccg cgcgaccttc
     2101 ttgccgccct cgtcggccac gtcgaacttc acgcgctgcc cgatgcggag gtaccggatg
     2161 ccggaaccct ccatcgaggt gacgtcgatg ccgacctcct gttggtcgtc ggtcagcaac
     2221 acgccggtgc gctgctcgcg gtcgtacgtc ttgatcgtcc cctgcggcac gggccctcct
     2281 accgctcgct ggcgaccgtc agtctgtcgg tgacgcgcat ctcgtggcaa gccgcgaccg
     2341 tgcgagagcg ccgcccccgc gcgagcagcg tgcgcacgtc atccggccgg tcgaggtcga
     2401 aggcgagcgc cggatccctc accgcggcga acggcaggcc gcgcgtctcg gccgcgtcct
     2461 gatgtttccg gaacgaatcg gttccgaacc gtggcgcgat cacggccgtc gggcgacgga
     2521 gcagcacgtt cgtcccgccg tcggagtccg acggcgccgc cacgaccgcg ccgagcgtgc
     2581 gcaggacccg ggccagcgcg tcggtgtgca gcagcgggag gtcgccgagc accacggcga
     2641 gcgcgtcggc ccccgcggcg tcctcctcga cctggcggac cgcggcgttc agtccggggt
     2701 cgcgttccgg caccgcgcgc gtccgccgcc tggccgcgac ctcgagcacc gcctcgtccg
     2761 gcgagacgac ccacgtgtgc cagccaggga tgtcgacaca ggcgtcgagg acgtcctcga
     2821 gcatcgcgag cgtgagcgcc gcccgctcga gaggggaaag cacagggatg agccgcccct
     2881 tcgctcgctc gagggatttc accggcaccg cgatgatgcg cacggcccac gatgctaccg
     2941 gccgaggcga cctcagcgtg cggcgctaaa agcgctcgac gtccttgacg gcgtcgccgt
     3001 gctggacgcc gccgaggaac gtcagccctc gcgcgacgat ccgtgccggc ctggcccgga
     3061 gctctcccga ctgcttccgg gactcgaccc agatggtctc ctcggttccc gtgcacctcc
     3121 cgctcgcgcg gccgatgccc tcgtagtcgg gcttgatctc gaccttggtc cggatctggt
     3181 acttctcgcc gtcggcgcac gtgaagcgga tgccggcgaa cgtcgtcccg agtccgaagt
     3241 gatacgtgat cccggctacc tcgaactgcg tgaagtgcgc ggaagccggc agcgccggcg
     3301 caacgacgcc gatcacgagc agcaggctca ggatgagcct tcgggtgcgc ttcacgtctc
     3361 ctccctcccg gcgcacggat gagcgccacg cggggattga tgcgcgagca ccgaagggcg
     3421 gtcaagagga tcgagggacg gcgcggctgg ctagaatggc ccgccggacg agcgggggtg
     3481 cgatgaggcg gcccgagccg tggatgaggt tcgcgacgag cgtcctggtt ccgcccatct
     3541 gggtctggtt cgattggcgg ttcgagggta cggagcatct ccctccagag gggcccgtgc
     3601 tcgtggccgc gaaccacatc tcgtacttcg acccgctcgc gcatggctat ctcgcggtca
     3661 aggccggccg ccgcccccgc ttcttgacca agatcgagct gtacgggaac ccgttcatgc
     3721 ggtcgctcct ccagggggcg cgccagatcc cggtccgtcg gggaagcggc gagcacggcc
     3781 cggtagaggc gacgatcaag gccctccgcg cagatgagct cgtcatcgtg taccccgagt
     3841 ccacgatcac acggaacccg gatttctcgc cgatgcaagg gaagacgggc gtcgcgcgcg
     3901 tcacgctgaa gaccggcgtg ccggtgcttc ccatggcggt ctggggatcg gcgcccgtgt
     3961 ggcagaaggg aggccggcgc agcctgaagt acggccgccc gatctgggtg aaggccggtc
     4021 cgccgctcga cttcacgcgg tacgaggacg gcgccgacga tcccgccgtg ctccgcaagg
     4081 tgaccgacga ggtgatggcg cagctgtcgc ttctcgtcga ggacatgcgc gcgcggtacc
     4141 cgaagcggtg gggcgagctc ccgccgcccg ccgagggcgg ttcgtgaacg gcgacccgcg
     4201 cgtcgccgtg atcggggccg gttcgtgggg aaccgcgttc gcgacgatcc ccgcggagaa
     4261 ggggatcgag acggttctgt gggcacgccg cgccgagctc gccgacgaga tcaacgcgcg
     4321 gcacgtgaac cccgactacc tgtccgagtt cgagctcccg ccgtcgctca gcgcgaccag
     4381 cgacgtcggg aaggcggtgg acggcgcggg cgccgtcgtg atggcggtcc catcccacgc
     4441 gttccgcggc gtctt
//
LOCUS       DASZOB010000224         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_32608, whole genome shotgun sequence.
ACCESSION   DASZOB010000224 DASZOB010000000
VERSION     DASZOB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32608"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VG709_03680"
     CDS             complement(<1..129)
                     /locus_tag="VG709_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWC32206.1"
                     /translation="MPLPDREPLSSVFLRFLRFGLLAWGGPFAQIAMVREELVERES"
     gene            complement(181..1269)
                     /locus_tag="VG709_03685"
     CDS             complement(181..1269)
                     /locus_tag="VG709_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWC32207.1"
                     /translation="MRAGEVMRRRMRASRLVGSGFGEPADVVRHHGAMQAQDYAPAKW
                     SIGQRARDLADADVDAAIADGSIVRTHVLRPTWHFVAREDLRWLLALTGPRVEQRSAP
                     RYRDLGLDARTLVRCERAIVRALEGGNHLTREAIGDALHVARIDTSGQRLPWVLMHCE
                     LEALICSGPRAGKHHTYALFDERVPAAAALDRDDALVELARGYLASHGPATVRDLSWW
                     SSLPVADLRRALHDLDAREESVDGVALWSIDGDSKRAPTLRGVRLLQPYDELVVGYTE
                     SRYLGDPRRDAALAAWRDRTLPNGIVLVNGRLGGFWRRRVAGKWLSADVVLYDDLSPA
                     QAPVLDAEIRRLSRFVGGPVRTEIRRLN"
     gene            1326..>1650
                     /locus_tag="VG709_03690"
     CDS             1326..>1650
                     /locus_tag="VG709_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWC32208.1"
                     /translation="MEAAFFDLDKTVISKSSSLALTRPFYRAGLVTRSQLLKGAYAQI
                     VYLMLGADEKRMDRAKEGMLALTKGWDQDEVEQIVREALEEVVDPYIYQEALDLIALH
                     QALGRG"
BASE COUNT          266 a          578 c          592 g          214 t
ORIGIN      
        1 cgactcgcgc tccacgagct cctcgcgcac catcgcgatc tgggcgaacg gaccccccca
       61 cgcgaggagg ccgaagcgca ggaaccgaag gaacacgctg gaaagcggct cgcggtcggg
      121 aagcggcacg gcaccggagc atgcgtcagc gacggacggc cggcaatcga ggcggacgga
      181 tcagttcagc cggcggatct cggttcggac gggccctccc acgaagcgcg acaggcggcg
      241 gatctccgcg tcgaggacgg gcgcctgggc gggggagaga tcgtcgtaca gcacgacgtc
      301 ggcgctcaac cacttcccgg cgaccctccg gcgccagaac ccgccgaggc ggccgttcac
      361 gagcacgatc ccgttcggaa gcgtccggtc gcgccaggcc gcgagcgcgg cgtcgcgccg
      421 cgggtcgccg aggtaccgag attccgtgta cccgacgacg agctcgtcgt acggctggag
      481 caagcggaca cctcggaggg tcggcgctcg cttcgagtcg ccgtcgatcg accagagagc
      541 gaccccatcg accgactcct cgcgcgcgtc gaggtcgtgc agcgcccgac ggaggtcggc
      601 gacgggaagg ctggaccacc agctgaggtc gcggaccgtc gcggggccat ggctcgcgag
      661 gtagccccgt gcgagctcga ccagcgcgtc atcgcgatcc aacgcggcgg cggcagggac
      721 gcgttcgtcg aagagcgcgt acgtgtggtg cttcccggcg cgcgggccgc tgcagatcag
      781 cgcttcgagc tcgcagtgca tcagcaccca cggaagccgc tgtcccgacg tgtctatccg
      841 ggcgacgtga agtgcatcac cgatcgcctc cctcgtcagg tggttgccgc cttcgagggc
      901 ccggacgatc gcgcgctcgc atcggacgag cgtccgtgcg tcgagaccga ggtcgcggta
      961 gcgcggcgcg ctgcgttgct cgacccgagg cccggtgagc gcgaggagcc atcgcaggtc
     1021 ctcgcgggcg acgaagtgcc acgtcgggcg gaggacgtgg gtccggacga tcgatccgtc
     1081 ggcgatcgcc gcgtcgacgt ccgcgtcggc gaggtccctc gcccgctggc cgatcgacca
     1141 tttggcgggc gcgtaatcct gcgcctgcat cgcgccgtgg tggcgcacga cgtcggccgg
     1201 ctcgccgaac cccgaaccga cgagccgcga cgcgcgcatg cgccgccgca tcacctctcc
     1261 cgcgcgcacg gcgcctcagg ctacggcatc gagaaccgag gcaagcccgg gcgtacgatg
     1321 gaggcgtgga ggccgccttc ttcgacctcg acaagacggt gatctcgaag tcgagctcgc
     1381 tggcgctgac gcgtcccttc taccgcgcgg gcctcgtcac caggtcgcag ctcctcaagg
     1441 gcgcctacgc acagatcgtc tacctcatgc tcggagccga cgagaaacgc atggatcgcg
     1501 ccaaggaagg gatgcttgcg ctcacgaagg ggtgggacca ggacgaggtc gagcagatcg
     1561 tccgcgaggc gctcgaggag gtcgtcgacc cgtacatcta ccaggaggcg ctcgacctca
     1621 tcgcgctcca ccaggcgctc ggccggggcg
//
LOCUS       DASZOB010000225         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_173969, whole genome shotgun sequence.
ACCESSION   DASZOB010000225 DASZOB010000000
VERSION     DASZOB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173969"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VG709_03695"
     CDS             complement(<1..322)
                     /locus_tag="VG709_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWC32209.1"
                     /translation="MTYDLTIERLIDGPREEVFDAFVDPDAQKALYANDEEDEWHVES
                     ELDLRVGGTWTIVFGKAGEEPFRETNVFTEVDRPRRLAFASTMFKGKYGGSFDTTVTV
                     TFEDR"
     gene            complement(319..657)
                     /locus_tag="VG709_03700"
     CDS             complement(319..657)
                     /locus_tag="VG709_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWC32210.1"
                     /translation="MVQYERALDATFSALSDPARRDILERLGRAPATISELAAPLGMT
                     LTGTKKHVRVLEDAHLVITQKVGRSRYCRVTPGRLEDAADWIETNRRRWERRLDRLEA
                     YIEQRKGAPR"
     gene            complement(732..2504)
                     /gene="thrS"
                     /locus_tag="VG709_03705"
     CDS             complement(732..2504)
                     /gene="thrS"
                     /locus_tag="VG709_03705"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWC32211.1"
                     /translation="MIDHDELEVLRHSTAHVMAQAVCDLWPGAKYAIGPPIHDGFYYD
                     FDLPVSLTPDDLGRIEERMREIVAERQPFRREELSRDEALKRFADNPYKVESIEGVEE
                     SEGAGGEVISVYRNDGWSDLCRGPHVASTSDLGAFKLLSIAGAYWRGDERRPMLQRIY
                     GTAWRTQEELDEYLRRREEAERRDHRRLGRELDLFSFPDELGAGLAVWHPRGGVFRKV
                     LEDYIRNLHLERGYEIVSTPHLARSVLWETSGHLEKFAENMYPAMHDESSDYYVKPMN
                     CPFHVLIYKSQTRSYRDLPMRLSELGTVYRLERPGVLHGLLRVRGLTQDDSHIICTPD
                     QLVDEILGVFELTLDIHRTFGFSDPVVTLSTQPGKAIGNPKMWERATDSLRLALDRSG
                     LAYEVAEGEGAFYGPKVDFHFHDAIGRLWQLTTVQCDFALPERFGMEYMAEDNLRHRP
                     VMVHRAILGSIERFTGVLVEHFGGAFPLWLAPEQVRIVPIADRHAEHAHGLASRLREA
                     DLRVSVDDSKQTVSRKVRDAQLMKVPYVLVVGDREIEGGTVAVRDRSGAEARGVPVER
                     FVEQAVEEARSRSLESGVVSALAA"
     gene            complement(2501..>3353)
                     /locus_tag="VG709_03710"
     CDS             complement(2501..>3353)
                     /locus_tag="VG709_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32212.1"
                     /translation="LYLTFVRRADEWLIASDSDLDDVTLFSGRKLWEFGPIVTRESEH
                     FLFVSHPVLESRAREILAAAERALGVVRARWPLPWSERLVILAPTTTEELRRILQASF
                     DLDVFVGFVYSGVDRSGDWRLAGHRMILNWPAFSSYPASVRDQIFEHELAHAATREYA
                     GPFTTAFVDEGMADWVASNVRDEQLEAELAAGTFDGELPRDFEFISGDDADILGAYEE
                     SGTAVEFAIGEFGIDAVARFYLRLGRARLAPGTPRYHVDRAARAAFGIGIDAFERRWA
                     EWLEETR"
BASE COUNT          534 a         1195 c         1112 g          512 t
ORIGIN      
        1 cgcggtcctc gaacgtcacg gtgacggtcg tgtcgaagct gccgccgtac ttccccttga
       61 acatcgtcga cgcgaaggcg agccggcgcg gccggtccac ctcggtgaag acgttggtct
      121 cgcggaacgg ctcctccccc gccttcccga acacgatcgt ccacgtccct ccgacgcgaa
      181 ggtcgagctc cgactcgacg tgccattcgt cctcttcgtc gttcgcgtag agggccttct
      241 gcgcgtccgg atcgacgaac gcgtcgaaga cctcctcgcg cggtccgtcg atcagccgct
      301 cgatcgtcag gtcgtaggtc acctcggcgc tcccttcctc tgctcgatgt acgcctcgag
      361 cctgtcgagg cggcgctccc accgccggcg gttcgtctcg atccagtcgg cggcgtcttc
      421 cagccggccc ggggtcactc ggcagtagcg tgagcggccg accttctgag tgatcacgag
      481 gtgcgcatcc tccaagacac ggacatgctt cttggtgccc gtgagcgtca tcccgagcgg
      541 cgccgcgagc tcgctgatcg tggccggagc gcggccgaga cgctcaagga tgtcgcgacg
      601 cgccgggtcg gagagagccg agaacgtcgc atcaagcgcc ctctcatact gaaccatccg
      661 gttcactatg agaggagatc gggcgtgtgt caagtcctgc ccggagaagt ccagagaccg
      721 ccgagcgcga gctacgccgc gagcgccgag acgacgccgc tctcgagcga gcgcgagcgc
      781 gcttcctcga ccgcctgctc gacgaagcgc tcgaccggca cgccgcgcgc ctcggcaccg
      841 gagcggtcgc gcacggcgac ggtaccgccc tcgatctccc gatccccgac cacgagcacg
      901 tacgggacct tcatcagctg tgcgtcgcgg accttacggg acaccgtctg cttggagtcg
      961 tcgacgctca cgcgcaggtc ggcctcgcgg agccgcgagg cgagcccgtg cgcgtgctcc
     1021 gcgtggcggt cggcgatcgg gacgatccgc acctgctccg gcgcgagcca gagcgggaac
     1081 gcgccgccga agtgctccac gaggacgccg gtgaaccgct cgatcgaccc gaggatcgcg
     1141 cggtggacca tcacgggccg gtgccggaga ttgtcctccg ccatgtactc catcccgaac
     1201 cgctccggga gcgcgaagtc gcactgcacg gtcgtgagct gccacagccg tccgatcgcg
     1261 tcgtggaagt ggaagtcgac cttcggcccg tagaacgcgc cctcgccctc cgcgacctcg
     1321 tacgcgagcc cgcttcggtc gagcgcgaga cggagcgaat ccgtcgctcg ctcccacatc
     1381 ttggggttcc cgatcgcctt ccccggctgc gtcgacagcg tcacgaccgg atcggagaag
     1441 ccgaatgtcc ggtggatgtc gagcgttagc tcgaacaccc cgaggatctc gtcgacgagc
     1501 tggtccggcg tgcagatgat gtgcgagtcg tcctgcgtca ggccgcgaac gcgcagcagc
     1561 ccgtgcagca cgcccgggcg ctcgagccgg tacacggtcc cgagctccga gagccgcatc
     1621 ggcaggtcgc ggtacgaccg cgtctgcgac ttgtagatca gcacgtggaa cgggcagttc
     1681 atcggcttca cgtagtagtc ggacgactcg tcgtgcatcg ccgggtacat gttctcggca
     1741 aacttctcga ggtggcctga ggtctcccag agcaccgagc gggcgaggtg gggcgtcgag
     1801 acgatctcgt acccgcgctc gaggtggagg ttccggatgt agtcctcgag caccttgcgg
     1861 aagacccctc cgcgcggatg ccacacggcc aggcccgcgc caagctcgtc cgggaacgag
     1921 aagagatcga gctcacgccc gagtcggcgg tggtcgcgac gctccgcctc ctcgcggcgg
     1981 cgcaggtact cgtccagctc ctcctgcgtc cgccatgccg tgccgtagat ccgctgcagc
     2041 atcggacgac gctcgtcgcc gcgccagtat gcgcccgcga tcgacagcag cttgaacgcg
     2101 ccgaggtccg acgtcgacgc gacgtgcggc ccgcggcaca ggtcggacca cccgtcgttg
     2161 cggtagacgc tgatgacctc tccgccggct ccctcggact cctcgacgcc ctcgatgctc
     2221 tcgaccttgt acggattgtc cgcgaagcgc ttgagcgcct catcccgcga gagctcctcc
     2281 cggcggaacg gttgccgctc cgcgacgatc tcccgcatcc gctcctcgat gcgcccgaga
     2341 tcgtccggcg tgagtgacac cgggagatcg aagtcgtagt agaagccgtc gtggatcggc
     2401 gggccgatcg cgtatttcgc gcccggccag aggtcgcaga cggcctgagc catgacgtgc
     2461 gcggtcgagt gccgcagcac ctcgagctcg tcgtggtcga tcatcgagtc tcctccagcc
     2521 actccgccca gcgccgctcg aacgcgtcga tgccgatgcc gaacgccgcg cgagccgcgc
     2581 gatcgacatg gtagcgcggg gtccccggcg ccagacgcgc gcgtccaagg cgaaggtaga
     2641 accgcgcgac cgcatcgatc ccgaactcgc cgatggcgaa ctcgaccgcg gtcccggatt
     2701 cctcgtacgc gccgagtatg tcggcgtcgt cgcccgagat gaactcgaag tcccgcggca
     2761 gctcgccatc gaacgtgccc gccgcgagct ccgcctcgag ctgctcgtcg cggacgttcg
     2821 acgcgaccca gtcagccatc ccctcgtcga cgaacgcggt cgtgaacggc cccgcgtact
     2881 cccgcgtcgc ggcgtgcgcg agctcgtgct cgaagatctg gtcgcgcacc gacgcgggat
     2941 aggaggagaa cgccggccag ttcaggatca tccggtgtcc ggcgagccgc cagtcgcccg
     3001 agcggtcgac gcccgagtac acgaacccca cgaagacgtc gaggtcgaag ctcgcctgga
     3061 ggatgcgacg cagctcctcg gtcgtcgtcg gcgccaggat gacaagccgc tccgaccacg
     3121 ggagcggcca gcgcgcccgg acgacgccga gcgcgcgttc cgccgcggcg aggatctcac
     3181 gcgcgcgaga ctcgaggacc gggtgggaga cgaagaggaa gtgctcgctc tcgcgggtca
     3241 cgatcggccc gaactcccag agcttccgac cggagaacag cgtcacgtcg tcgaggtcgg
     3301 agtcggatgc gatcagccac tcgtcggctc ggcggacgaa cgtgaggtag agc
//
LOCUS       DASZOB010000226         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_106171, whole genome shotgun sequence.
ACCESSION   DASZOB010000226 DASZOB010000000
VERSION     DASZOB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106171"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..856
                     /locus_tag="VG709_03715"
     CDS             227..856
                     /locus_tag="VG709_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWC32213.1"
                     /translation="MDLAEAMETQRAIRKLLPDPVDDEVLLRCLRLATKAPTGGNRQG
                     VEFLLVRDDRKKAALGRLYRIAWSVYGGAGRLLYGRREQQRKIMDAVDWQLAHWQKIP
                     VLVVVCLRGLPLPFPSVARTARYGSVYPAIQNFLLAARAEGLGAALTTLPLWSRIAVH
                     RVLDIPWSVEPVAVIPVGWPRGRYGPTSRRAVERSIHVDRYGNRPFESR"
     gene            926..1408
                     /locus_tag="VG709_03720"
     CDS             926..1408
                     /locus_tag="VG709_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32214.1"
                     /translation="MAATRKGTTRKRTTARKTSGGKTTAGRKKTTARKGTAKRASARK
                     ATTKRTAARKGTSTRKRTTRKATSPRKKTSARKGTAKRTTRRPSPERPAQPEMAPESG
                     PSPETGGAESGGETRGLLGGLFGGGGRPEGTEETQRGGAGEPGGASESTEREAREPGV
                     "
     gene            complement(1405..2193)
                     /locus_tag="VG709_03725"
     CDS             complement(1405..2193)
                     /locus_tag="VG709_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWC32215.1"
                     /translation="MPERSAALVVALWLAASACERGIGRLLPGGGRGDGGNASETGPG
                     RLSARSGRPTERPPAAGEHRLPIGGKRNGVVQIPAGYERSRPAPLVIVLHGAGGEAAR
                     RLFAKPYERFGLVVVAPESRGRNWDLLEGGGFGSDVAWLDRVLRWTFSRFRIDPNRVA
                     IAGFSDGASYALSLGLSNGDLFTHVMAFSPGFVAPAERVGRPSIFVSHGRDDPVLSFE
                     NARDEIVPRLRDWGYGVRFVPFDGAHDVPLDVSRGAVRWFLRAR"
     gene            2262..>2729
                     /locus_tag="VG709_03730"
     CDS             2262..>2729
                     /locus_tag="VG709_03730"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32216.1"
                     /translation="MRELPTGVVTFLFTDIEGSTRLLDELGSGYSAVQDAHMAIMREA
                     IASNRGLELRTEGDAFFAVFRTPVDAVGAAVAAQRGLTRHEWPHGRPLRVRMGIHTGE
                     GRLGGDDYLGIDVNRAARIAAAGHGGQVLVSDATRVLVERDLPGGVTLRDLGSH"
BASE COUNT          437 a          974 c         1012 g          306 t
ORIGIN      
        1 ggagaggacc gcggctcgga ggacggtgga gggagatccc tgacggaacc gccctccgcg
       61 cgcggcagcg tgagggcgcc gacgagcgct ccggtcagcg tcgcgatgac ggccgccgcg
      121 acgaggagca gcgctctctg actcatgtca acgtgatacc cgagcgccgg taagtcgcgg
      181 taagcgacgg ccaaaggccc ggctaagcct cgggtacagt ccctgcgtgg acctcgctga
      241 ggctatggag acgcaacggg cgatcaggaa gcttctcccg gatccggtcg acgacgaggt
      301 gctcctccgc tgcctccggc ttgcgacgaa ggccccgacc ggcggcaacc gccagggcgt
      361 cgagttcctc ctcgttcgcg acgaccgcaa gaaggcggcg ctcggacgcc tctaccgcat
      421 cgcgtggtcg gtgtacggcg gagccggtcg cctcctgtac gggcggcgcg agcagcaacg
      481 caagatcatg gacgccgtcg actggcagct ggcgcactgg cagaagatcc cggtcctcgt
      541 cgtcgtctgc ctgcgcgggc tcccgctccc cttcccgtcc gtcgcgcgaa cggcgcggta
      601 tggctcggtc taccctgcga tccagaactt cctgctcgcg gcgcgcgccg aggggctcgg
      661 ggccgcgctc acaacgctcc cgctctggag ccggatcgcg gttcaccgcg tgctcgacat
      721 cccgtggagc gtcgaacccg tcgccgtgat cccggtcggc tggccgcgcg gcagatacgg
      781 tccgacgtcg cggcgagccg ttgagcgctc catccacgtc gatcggtacg ggaaccggcc
      841 gttcgagtcg cggtgacgcg aggggcgcct tgcctcccac cgcgcgtcgg tggcaccatc
      901 gtcgtgggag cgaaaggagg ccaaagtggc cgcaacgcgc aagggcacga ctcgtaagcg
      961 aacgacggcg cggaagacgt cgggcggcaa gaccaccgcc ggacgcaaga agacgaccgc
     1021 gcggaagggc accgcgaagc gcgcgtcggc gcggaaggcg acgaccaagc gcacggcggc
     1081 ccgcaaggga acgtcgacgc gcaagcgcac gacccgcaag gcaacgtcgc cgcggaagaa
     1141 gacgtccgcg cggaagggaa cggcgaagcg gacgacgcgg cgcccgtcgc ccgagcggcc
     1201 ggctcagccg gagatggcgc cggagtccgg cccgtcgccc gagaccggag gcgcggagag
     1261 cggcggcgag acgcgcggcc tgctcggcgg gctcttcggc ggaggcggaa ggccggaagg
     1321 gacggaagag acgcagcggg gcggagccgg cgagcccggg ggcgcgtccg agtcgaccga
     1381 gcgggaggcg cgcgagccgg gcgtctagcg cgctcgaagg aaccagcgga cggcgccgcg
     1441 cgacacgtcg agcggcacgt catgggcgcc gtcgaagggg acgaaccgca cgccgtaccc
     1501 ccagtcccgg agtcgcggga cgatctcgtc gcgggcgttc tcgaacgaga ggacggggtc
     1561 gtctctccca tgcgagacga atatcgacgg ccgcccgacg cgctccgcag gcgcgacgaa
     1621 gcccggggag aacgccatca cgtgcgtgaa gaggtcgccg ttcgagagcc cgagcgagag
     1681 cgcatacgat gcgccgtccg agaaccccgc gatcgcgact cggttcgggt cgatgcggaa
     1741 gcggctgaac gtccaacgca ggacgcgatc gagccacgcg acgtccgacc cgaacccgcc
     1801 gccctcgagc aggtcccagt tccggccgcg cgactcgggc gccacgacga cgaggccgaa
     1861 ccgctcgtag ggcttcgcga acaacctccg cgcggcctcg ccgccggcgc cgtggagcac
     1921 gatcacgagc ggcgccggcc gcgaccgctc gtacccagcc gggatctgca cgacgccgtt
     1981 ccgcttgccc ccgatcggaa ggcggtgctc gccggccgca ggagggcgct cggtcggacg
     2041 ccccgatctc gcggagaggc gccccgggcc ggtctcgctc gcgttcccgc cgtcaccgcg
     2101 cccgccgccg ggcaggagcc ggccgatccc gcgctcgcaa gccgacgccg cgagccacag
     2161 cgcgacgacg agggcggccg agcgctcagg cacgcgaccg accctacccg cgccgggcgg
     2221 ggcatcaagc cgagctacga cgtagcatcg gtctccgcac catgagggag cttcccaccg
     2281 gcgtcgtcac gttcctgttc accgacatcg agggctcgac gcgcctcctc gatgagctcg
     2341 ggtccggcta ctcggcggtg caggacgcgc acatggcgat catgcgagag gcgatcgcct
     2401 cgaaccgagg cctcgagctt cgcaccgagg gagacgcgtt cttcgcggtg ttccgcacgc
     2461 cggtcgacgc ggtcggagcc gccgtcgcgg cgcagcgtgg gctcacgcgg cacgagtggc
     2521 cccacggacg gccgctgcgc gttcggatgg ggatccacac tggggagggc cggctcggcg
     2581 gcgacgacta cctcggcatc gacgtgaacc gggccgccag gatcgcggcg gcagggcacg
     2641 gcggtcaggt cctcgtctcg gacgcgacgc gcgtactcgt cgagcgcgac ctcccgggcg
     2701 gggtgacgct ccgggacctc ggcagccac
//
LOCUS       DASZOB010000227         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125765, whole genome shotgun sequence.
ACCESSION   DASZOB010000227 DASZOB010000000
VERSION     DASZOB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125765"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2253)
                     /locus_tag="VG709_03735"
     CDS             complement(<1..2253)
                     /locus_tag="VG709_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HWC32217.1"
                     /translation="MSGSPALSRRFARLFAGAVAVLICLALAVPPGATASRRPEATEP
                     VIFDGEHGLEEFDARRGSVDPSPEQIAAADALGATRVAWNRFGTPHSLIRHGGYLATG
                     LPRDPVAAARSFLSRNRTLFRVSRPAVSSLAVVNVARLGGAARAVLFRQRFGDLAAGH
                     DGLVTVGVVRGKVFYVSSSLTGDTALAGSVRVSPTDAWLRAAADIGRDANAFTTGAAR
                     EQRGWSVFRVEGIAQPQRARLVAVPTPQNGVRPAFHTLFVDVRDGKAEGYAHFVDAET
                     GEVLIRQNLVEQQADQQPQAPTWKVFPAYPIPPRLEAFPWNVPSDDIRELWCWGADDK
                     TLLRRCDRVVRNSASRVPWDVFPPAGVPSFTTGGNNAHSGEAWTTPFTPGATGYRPVS
                     PSRHYVYPWTNQWYEQRCDPTTFVSPQRVDIDAAAANLFVMHNRMHDWSYHLGFTEQN
                     WNGQQYNYGTRPLGERDPILGDVQAGALTGGFPAYLGRDNANMLTLPDGIPSITNMYL
                     WQPIRGAAYLPCVDGDYDMAVIAHEYGHMIENRMIGKGDRRAGHHAGAMGESHSDLVA
                     MEILNEYGFVPVADENPYAVGAYVTAEKETGVRNYAMNWPSTGLPRPGTNVDVNPLNF
                     SDMGYDITGPQVHADGEIWSATNFDIRELLVKKYDDRSPVSDQELQRRCAEGQVPVRK
                     CPGNRRWVQLVFDSFLLMPTAPSMLDARDAQLAADVARFNGANQRELWLAFARRGFGE
                     AAFSSNGTGDS"
     gene            complement(2439..2807)
                     /locus_tag="VG709_03740"
     CDS             complement(2439..2807)
                     /locus_tag="VG709_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32218.1"
                     /translation="MDVAHCDGSSAAGCVRFETWPRDSYVRVSVTDASNTGPVLASVA
                     QDLDADGAVEPDEHLRTFCGRSKRLWIRRPGKSLTVFVYEARGGVYFTYSLLCPVCPY
                     VVRCTDRVVATTGTVKARFS"
     gene            complement(2956..>3368)
                     /locus_tag="VG709_03745"
     CDS             complement(2956..>3368)
                     /locus_tag="VG709_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWC32219.1"
                     /translation="DVLRGGDGNDTLTGGGGNDSIDGSAGTDTASYSGSSGGVTANLS
                     AGAPQVSSDGTGGSDTLSGIESLTGSSTGSDSITGSSGANVLDGLGGNDNLYGLSGND
                     TLRGGSGTDRLDGGDGTDKCVDKDSNTTRVSCEG"
BASE COUNT          501 a         1161 c         1189 g          517 t
ORIGIN      
        1 cgagtctccg gtcccgttgc tggagaatgc ggcctcgccg aagccgcggc gcgcgaacgc
       61 gagccacagc tcgcgctggt tcgctccgtt gaagcgcgcg acgtcggccg cgagctgcgc
      121 gtcgcgggcg tcgagcatcg aaggagccgt cggcatcagc aggaacgagt cgaagacgag
      181 ctggacccac cgccggttcc cggggcactt cctcaccggg acctggccct cggcgcaacg
      241 ccgctgcagc tcctggtcag acaccggcga gcggtcgtcg tacttcttga cgagcagctc
      301 tctgatgtcg aagttcgtcg cgctccagat ctcgccgtcg gcgtggacct gcggcccggt
      361 gatgtcgtac cccatgtcgc tgaagttcag cgggttcacg tcgacgttgg tgccgggacg
      421 cggaagtccc gtcgacggcc agttcatcgc gtagttgcgg acgccggtct ccttttcggc
      481 cgtcacgtac gcgccgaccg cgtaggggtt ctcgtcagcg accgggacga agccgtactc
      541 gttgaggatc tccatcgcga cgaggtcgga gtggctctcg cccatcgcgc ccgcgtggtg
      601 cccggcgcga cggtcgccct tgccgatcat ccggttctcg atcatgtggc cgtactcgtg
      661 ggcgatgacg gccatgtcgt agtcgccgtc gacgcacggc aggtaggcgg cgccgcggat
      721 cggctgccac aggtacatgt tggtgatcga ggggatcccg tccgggagcg tcagcatgtt
      781 cgcgttgtcg cgccccaggt aggcggggaa cccaccggtg agcgccccgg cctggacgtc
      841 gccgaggatc gggtcacgct cgccgagcgg ccgggtgccg tagttgtact gctggccgtt
      901 ccagttctgc tcggtgaacc cgaggtggta cgaccagtcg tgcatgcggt tgtgcatcac
      961 gaagaggttg gcggcggcgg cgtcgatgtc cacccgctgc ggcgacacga acgtcgtcgg
     1021 atcgcaccgc tgctcgtacc actgattcgt ccacggatag acgtagtgcc ggctcgggga
     1081 gaccggccgg tatccggtcg cacccggcgt gaacggcgtg gtccacgcct cgcccgagtg
     1141 ggcgttgtta cccccggtgg taaagctcgg cacgcccgcc ggcgggaaga cgtcccaggg
     1201 gacacgcgag gcggagttcc gcactaccct gtcgcacctg cggagcagcg tcttgtcgtc
     1261 tgccccccag caccagagtt cgcggatgtc gtcgctcggg acgttccacg ggaaggcctc
     1321 gagccgcggc gggatcgggt aggccgggaa gaccttccaa gtcggggcct gaggctgctg
     1381 atccgcttgc tgctcgacga ggttctggcg gatcaggacc tcgccggttt ccgcgtcgac
     1441 gaagtgtgcg tagccctcag ccttgccgtc gcgcacgtcg acgaagagcg tgtggaaggc
     1501 gggccggaca ccgttctgcg gggtcggcac ggcgacgagg cgcgcgcgct gaggctgcgc
     1561 gatcccctcg acccggaaga cgctccagcc ccgctgctcc cgagcggcac cggtcgtgaa
     1621 cgcgttcgcg tcccggccga tgtcggccgc cgcccggagc cacgcatccg tcggcgagac
     1681 gcggacgctg cccgcgagcg cggtgtcgcc cgtgagcgaa gacgacacgt agaacacctt
     1741 cccgcgcacg accccgaccg tgacgagccc gtcgtgaccg gcggccaggt ctccgaagcg
     1801 ctgcctgaac agcacggctc gcgccgcacc gcccaggcga gcgacgttca cgacggcgag
     1861 actcgagacg gcgggccggg agacgcgaaa cagcgtccgg ttccgcgaga ggaaggaccg
     1921 agcggccgcg accgggtctc gagggagccc cgttgcgagg tagcccccat ggcggatcag
     1981 cgagtgcggg gtgccgaagc ggttccacgc gacgcgggtc gccccgagcg cgtccgccgc
     2041 ggcgatctgc tcgggcgacg ggtcgacgga cccgcgtcgg gcgtcgaact cctcgaggcc
     2101 gtgctcgccg tcgaagatga cgggctcggt cgcctccggc cggcgcgaag ccgtggcacc
     2161 cggcggcacg gccagcgcga gacatatgag gacggcgacg gcacctgcga acagccgggc
     2221 gaaacggcgg gaaagggcgg gcgaaccgct cacgggcgtg acctccctcg gtccgggcta
     2281 catggtgtgt tcgcccttaa tccccgattt cctacctggc tgcgagcgaa actagtggac
     2341 ctcttcggtt ggctgccatg gcgcccacca ctccaggcga ggtacaagat cgccgacgac
     2401 ttccatcctc catgcgtcct tgctccttga aacccgcatc acgagaagcg cgctttcacc
     2461 gttccggtcg tcgcaactac acggtcagtg cagcgaacaa cgtaggggca gacaggacac
     2521 aacaacgagt acgtgaagta gacgccaccg cgcgcctcgt acacgaagac cgtgaggctc
     2581 ttgcccgggc gccggatcca cagtcgcttc gaccgcccgc agaaggtcct gaggtgctcg
     2641 tcgggctcga cggcgccgtc cgcatcgagg tcctgggcga ccgacgcgag gaccggaccg
     2701 gtgttcgatg catccgtcac gctcacgcgg acgtaggagt cgcgcggcca cgtctcgaac
     2761 cggacgcagc cggcggcgga agaaccgtcg cagtgcgcta cgtccacggg ccgagagaac
     2821 ggaaggagcc gggctccggg cgccgcgtac gtgctcgtcg ccgtgcgggt cccgcgctgc
     2881 actccggcgg aggcctcgac agcagtcgcg agcgtggcgg ccgtgagggc tgccacgatg
     2941 accacacgtg tccgcctagc cctcacagga gaccctcgtc gtgttcgagt ccttgtcgac
     3001 gcacttgtcg gtgccgtcgc ccccgtcgag gcggtccgtg cccgagccgc cgcgcagcgt
     3061 gtcgttgccg gagagcccgt agaggttgtc gttgccgccg aggccgtcga ggacgttggc
     3121 cccggacgat cccgtgatcg agtcagagcc ggtgctcgac ccggtcaggc tctcgatccc
     3181 cgagagcgtg tcgctccccc cggtcccgtc ggaggagacc tggggcgcgc ccgccgacag
     3241 gttcgcggtg acgccgcccg aggagccgct gtagctcgcg gtgtccgtcc cggcgctccc
     3301 gtcgatggag tcgttgccgc caccgccggt cagcgtgtcg ttgccgtcac cgccgcgcag
     3361 cacgtcgt
//
LOCUS       DASZOB010000228         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_166345, whole genome shotgun sequence.
ACCESSION   DASZOB010000228 DASZOB010000000
VERSION     DASZOB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166345"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VG709_03750"
     CDS             <1..625
                     /locus_tag="VG709_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWC32220.1"
                     /translation="TGLESTRQMVFAAFPRSGVPPRFRDYNEYAEVVGQLEKTGCIAD
                     YTHIWWDIRPHPKWGTIEVRVMDAVTRVEHAVAIAAYIQALVKHYSEASDGGQDVPSF
                     HRMLVTENKWLAGRYGLEAPVMDLLTGRRNRVPVAQLIRRTLREIEPHARELGSDREL
                     DGIREILAHGNGADRQRRVFNANRDVVEVAREIAEATERVEPERAAA"
     gene            622..759
                     /locus_tag="VG709_03755"
     CDS             622..759
                     /locus_tag="VG709_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32221.1"
                     /translation="MIGVGEPAPNAEVWLAPHRPVLLHHLAPPGEALLLLFYVFDWSS
                     T"
     gene            769..1086
                     /locus_tag="VG709_03760"
     CDS             769..1086
                     /locus_tag="VG709_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWC32222.1"
                     /translation="MLLLRDRRAEREAARVSPFGISRDSAWSHVAWRQALDLEIPLLS
                     DWNGEAVRAFGVVREWRRLRDVAERSAFLVDGERVVRFAKRYGIDDVPDMDELVSAAR
                     SLA"
     gene            1096..1518
                     /locus_tag="VG709_03765"
     CDS             1096..1518
                     /locus_tag="VG709_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SSI family serine proteinase inhibitor"
                     /protein_id="HWC32223.1"
                     /translation="MRLLRLAAAVPLVLALAACGGADEPPADEPDEPAPATVLDVTVW
                     PTGRDGEKRSATLTCDPVGGTHPRADAACAALAEAPGALEDVPADLLCTQQYGGPQYA
                     EVRGTVAGEPVSTALGRQNGCEIARWNALRPLLDIEDG"
     gene            1554..>1765
                     /locus_tag="VG709_03770"
     CDS             1554..>1765
                     /locus_tag="VG709_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HWC32224.1"
                     /translation="MRADGWLPLRDYAAIGDGRTLALVGRDGSIDWLCLPDLDSPSVF
                     ARVLDRRRGGFFRLAPAGRWDVVRRYV"
BASE COUNT          236 a          640 c          651 g          238 t
ORIGIN      
        1 gacgggtctc gagtcgacga ggcagatggt cttcgccgcg ttcccgcgtt cgggcgtgcc
       61 gccgcgcttc cgggactaca acgagtacgc cgaggtcgtc ggtcagctcg agaagaccgg
      121 ctgcatcgcc gactacacgc acatctggtg ggacatccgc ccacacccga agtgggggac
      181 gatcgaggtc cgcgtgatgg acgccgtcac gcgcgtcgag cacgccgtgg cgatcgccgc
      241 gtacatccag gcgctcgtca agcactactc ggaggcctcc gacgggggcc aggacgttcc
      301 gtccttccac cgtatgctcg tgacggagaa caagtggctc gccgggcggt acggcctcga
      361 ggcgcccgtg atggacctcc tcaccggccg gcggaaccgc gtgcccgtcg cccagctcat
      421 ccgccgcacg ctgcgcgaga tcgagccgca cgcacgcgag ctcggatcgg accgcgagct
      481 ggacggaatc cgcgagatcc tcgcgcacgg caacggcgcc gaccgccagc gccgcgtctt
      541 caacgcgaac cgcgacgtcg tcgaagtcgc ccgcgagatc gccgaggcga cggagcgcgt
      601 ggagcccgag cgcgcggccg cgtgatcggc gtcggggagc cggccccgaa cgccgaggtc
      661 tggctcgcgc cgcacaggcc cgttctcctg caccacctgg cgccgccggg cgaggcgctg
      721 ctcctcctct tctacgtctt cgactggtcg agcacctgaa ctgacgagct gctcctcctg
      781 cgtgacagga gggcggagcg cgaggccgcg cgcgtctcgc cgttcggaat ctcgcgcgac
      841 agcgcgtggt cgcacgtcgc gtggcgacag gcgctcgacc tcgagatccc actgctctcg
      901 gactggaacg gcgaggcggt tcgcgccttc ggcgtcgtcc gcgaatggcg ccggctgcgc
      961 gacgtcgccg agcggtccgc gttcctcgtg gacggcgagc gcgtcgtccg cttcgcgaag
     1021 cgctacggaa tcgacgacgt gcccgacatg gacgagctcg tgtcggccgc gcgctctctc
     1081 gcgtaacgtg gccgcatgcg gttgctccgg ctcgcggcgg ccgtcccgct cgtcctcgcg
     1141 ctggcagcgt gcggcggcgc ggacgagcca ccggcggacg agccggacga accggcgccc
     1201 gcgacggtgc tcgacgtgac ggtctggccg acaggccggg acggcgagaa gcggagcgcg
     1261 accctcacgt gcgacccggt cggcggcacg cacccgcggg cggacgcggc gtgcgccgcg
     1321 ctcgccgagg cgccgggggc gctcgaggac gtgccggccg acctgctttg cacccagcag
     1381 tacggcggac cgcagtacgc ggaggtgcgc gggacggtgg ccggcgagcc ggtctcgacg
     1441 gcgctcggcc ggcagaacgg ctgcgagatc gcccgctgga acgcgctccg gccgctgctc
     1501 gacatcgagg acgggtagcc ggtcacgagc cctcgccggc gagcacgggc gtgatgcgcg
     1561 cggacgggtg gcttccgctc cgcgactatg cggcgatcgg cgacgggcgg acgctcgccc
     1621 tcgtcggccg ggacggctcg atcgactggc tctgcctgcc cgacctcgac tcgccgagcg
     1681 tcttcgcccg cgtcctcgac cggcgacgcg gagggttctt caggctcgcg ccggcagggc
     1741 gatgggatgt cgtgcgccgt tacgt
//
LOCUS       DASZOB010000229         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_25156, whole genome shotgun sequence.
ACCESSION   DASZOB010000229 DASZOB010000000
VERSION     DASZOB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25156"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VG709_03775"
     CDS             <1..972
                     /locus_tag="VG709_03775"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HWC32225.1"
                     /translation="ADLPSWRPPSYNEADVLDKPDWLHAIPLLTAEESNALDAFRIAQ
                     TRTLLAVDDAVAEIMTALVRAGRLANTFIVFTSDNGFLWGEHRLKGKRAPYDEAIRVP
                     FVVRYDPLVARARSDPRLVLNVDIAPTVAALAGAAAPGAEGRSIEPLLHGADVLWRTG
                     FLVENRGRPRTYCALRRADDSFVTYATGERELYDAAGDAYQLTNLAGDPARSSTAAAL
                     RRRLARLCNPPPPGLSRELLCTREGTPSADVLRGTARYDVLCGRRGKDHLYPRGGEDW
                     VYAGRRNDLVDSRDGHADVIRCGPGKDVVRADRRDRLRADCERILLP"
     gene            complement(969..1514)
                     /locus_tag="VG709_03780"
     CDS             complement(969..1514)
                     /locus_tag="VG709_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWC32226.1"
                     /translation="MRVFVSLVVLLGVAGAGFAYTEETKPGWYVRLRYPLEYEHIVVG
                     HARNYELDAALLAAVIYRESKFEADARSSSGAIGLMQLLPATAKGIAVHTGGSRFRVA
                     DLLDPEINVRYGSFYLRRLLRKYDDVALALAAYNAGQANVDEWIDAGDGIQFPETREY
                     VDDVLETRDAYAKAYAEELRL"
BASE COUNT          250 a          585 c          573 g          232 t
ORIGIN      
        1 gccgacctgc cttcgtggcg cccaccgagc tacaacgagg ccgacgtact cgacaagccc
       61 gattggctgc acgccattcc gctgctaacg gccgaggaga gcaatgcgct cgacgcattc
      121 cggatcgccc agacgcggac cctcctcgcg gtcgacgacg cggtcgccga gatcatgacc
      181 gcgctcgttc gcgccggccg cctggcaaat acgttcatcg tcttcacgtc cgacaacggc
      241 ttcctgtggg gcgagcaccg cctcaagggg aagcgcgcgc cgtacgacga agcgatccgc
      301 gtccccttcg tcgtccgcta cgacccgctc gtcgcgaggg cgaggagcga tccccgtctg
      361 gttctcaacg tcgacatcgc ccccacggtc gccgcgttgg cgggcgcagc cgcgccgggc
      421 gccgaggggc ggagcatcga gccgctcctc cacggagccg acgtgctgtg gcggacgggc
      481 tttctcgtcg agaaccgcgg caggcctcga acgtactgcg cgctccgccg agcggacgac
      541 tcgttcgtca cgtacgcgac gggcgagcgt gagctctacg acgcagcggg cgacgcgtac
      601 cagctcacga acctcgccgg ggatcccgcg cggtcgagca ccgcggccgc gctgcgcaga
      661 cgcctcgcac ggttgtgcaa ccctcccccg cccggcttgt cgcgcgaact cctctgcacg
      721 cgggagggga cgccgagcgc cgacgtgctg cgcggcacgg cccgctatga cgtcctctgc
      781 gggcggcgcg ggaaggacca cctctacccg cggggcggcg aggactgggt ctacgccggc
      841 cgccgaaacg acctcgtcga ctcgcgcgac ggccacgcgg acgtgatcag atgcgggccg
      901 ggcaaggatg tcgtccgggc ggatcggcgg gaccggctgc gggccgactg cgagcgaatc
      961 ctcctgccct agagcctcaa ctcctcggcg tacgccttcg cgtaggcgtc gcgcgtctcc
     1021 aggacgtcgt ccacgtactc gcgggtctcc gggaactgga ttccgtcgcc ggcgtcgatc
     1081 cactcgtcga cgtttgcctg ccccgcgttg tacgcggcga gcgcaagcgc gacgtcgtcg
     1141 tacttccgca gcaggcgacg caggtagaac gagccgtagc ggacattgat ctcggggtcg
     1201 agcaggtcgg cgacgcggaa gcgcgagccg cccgtgtgga cggcgattcc ctttgccgtg
     1261 gcgggcagga gctgcatgag gccgatcgcg cccgacgagg agcgtgcgtc cgcctcgaac
     1321 ttgctctcgc ggtagatgac ggcggcgagc agggcggcgt cgagctcgta gttgcgcgca
     1381 tggccgacga cgatgtgctc gtactcgagc gggtagcgca gccgcacgta ccacccgggc
     1441 ttcgtctcct cggtgtacgc gaacccggcg ccggcgacgc cgagcaggac gacgagcgag
     1501 acgaagaccc tcacgaaccg gagagcttgc cgaccacgtc ggctacgaag gcgtcgagct
     1561 cggcgagcga gccgtcgttg acgaaggcga agtcggcacg ccgcagcttc tcctcgtcgg
     1621 gcatgagccg ccgccgccgg
//
LOCUS       DASZOB010000230         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_185183, whole genome shotgun sequence.
ACCESSION   DASZOB010000230 DASZOB010000000
VERSION     DASZOB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185183"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..349)
                     /locus_tag="VG709_03785"
                     /pseudo
     CDS             complement(1..349)
                     /locus_tag="VG709_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333642.1"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II; functions in riboflavin synthesis;
                     converts GTP to
                     2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine;
                     converts ribulose 5-phopshate to 3,4-dihydroxy-2-butanone
                     4-phosphate; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
     gene            638..1273
                     /locus_tag="VG709_03790"
     CDS             638..1273
                     /locus_tag="VG709_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HWC32227.1"
                     /translation="MTVSAPRGMSVVVDWDGTVTETDTLALVMREFGDLSVYRETGRE
                     MGRSLTHDEALAASFATVRADLDDVVGWLLANVRIRAGFADLVREHRPLVVSSGFHEL
                     IDPILRREGLAVEVLANRVEADPDGWRAVFREEAACEECGEACKRASLPSPDVVYVGD
                     GYSDRCAALAARRVFATGPLALALARRAVPYEPFGDFHDGVAALRADEGDR"
     gene            1270..>1558
                     /locus_tag="VG709_03795"
     CDS             1270..>1558
                     /locus_tag="VG709_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005533210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HWC32228.1"
                     /translation="MKARVVRFVAPYRVELAEVGVPDPQDRDVLVRAEYSGISGGTEL
                     LAYRGDVDPELPLDETLGALGGTFAYPFAYGYSAVGTVEASGGTVPEGSTVF"
BASE COUNT          186 a          567 c          586 g          219 t
ORIGIN      
        1 cgccgaggcg ggcgaggcac gccgcggcgg gaccgtagtc gcgagcgtcg gcgggaaagc
       61 cgaggtggcg gttggcgtca agcgtgtcga gaccctcgtc ctggagcatg tacgcgcgca
      121 gcttgtggac gcggccgatt ccgcggccct cgtgccccgt cgcgtacacg aggacgccgc
      181 ggccctcggc ggcgaggctc cgaagtgcgg tctggagctg gaggccgcag tcgcagcgga
      241 gggagccgag cgcatccccg gtgaggcact ccgagtggag tcgcgtgagg acgcgccgtc
      301 cgtcgccgag gtcgcccttg acgagcgcga ggtagacgtg gcccgccggc agctcgaacg
      361 cgtgcacgtc gaactcgccc gccggcgtcg cgatcggcac ccgcgccacc tcgaccggct
      421 ctgcgcgcgc gggcagggcc ggcgcggcgg cgtctctcct tccgtcgcga cccactagtc
      481 tggtgcgcag cgcacggttg cctctcccac gctcactgca tccctcccgt tcggcgcgct
      541 gcgcgcggtc gagcccgacg ccgctgcatg gcctccagcg cggtgggccg ggcgatcgta
      601 ccgaagaaga gccgcggcgc gaggggccgc cgccggcgtg accgtctccg cgccgcgcgg
      661 aatgagcgtc gtcgtcgact gggacggcac cgtcacggag acggacacgc tcgcgctggt
      721 catgcgcgag ttcggcgatc tctccgtgta ccgggagacc ggccgcgaga tggggcgttc
      781 gctcacgcac gacgaggcgc tcgcggcgag cttcgccacc gtgcgggccg acctcgacga
      841 cgtcgtcggc tggttgcttg cgaacgtgcg catccgcgcg ggcttcgccg acctcgtccg
      901 cgagcatcgt cccctcgtcg tctcgagcgg gtttcacgag ctcatcgacc cgatcctgcg
      961 gcgggaggga ctcgccgtcg aggtgctcgc caaccgcgtc gaggccgacc ccgacgggtg
     1021 gcgcgccgtc ttccgcgagg aggccgcgtg cgaggagtgc ggcgaggcgt gcaagcgagc
     1081 ctcgctcccg tcgcccgacg tcgtctacgt cggcgacggc tactccgacc gctgtgcggc
     1141 gctcgccgcg cgacgcgtct tcgcgaccgg gcccctggcg ctcgcgctcg cgcggcgcgc
     1201 cgtcccgtac gagccgttcg gcgacttcca cgacggcgtc gcggccctcc gggccgacga
     1261 gggagaccgg tgaaggcgcg cgtcgtccgg ttcgtcgcgc cctaccgcgt ggagctggcc
     1321 gaggtgggcg ttcccgaccc gcaagaccgc gatgtcctcg tgcgcgcgga gtactcgggg
     1381 atcagcggcg gcaccgagct gctcgcctac cgcggcgatg tggatcccga gcttccgctc
     1441 gacgagacgc tcggcgcgct cggcggcacc ttcgcgtacc ccttcgccta cggctacagc
     1501 gcggtcggga cggtggaggc atcgggcggg acggttcccg agggatccac ggtgttcg
//
LOCUS       DASZOB010000231        10657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_125884, whole genome shotgun sequence.
ACCESSION   DASZOB010000231 DASZOB010000000
VERSION     DASZOB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125884"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VG709_03800"
     CDS             complement(<1..890)
                     /locus_tag="VG709_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWC32229.1"
                     /translation="MGLGAEAPPSLAARVARSAVAVAAAFVLAACTKPPVESDPRGGE
                     DLSFPTRPSLRIATQDVESIDPVELETLDEIVVADQVFDGLVAYEPETTELVPSAAQS
                     WEVRDGGRTFVFHLRGGATFHDGSPVQAEDFVFAWNRLADPAAAAPFAFLLESVAGYD
                     SYQSDVQVRELEGVRALDATTLEVRLSSPWPDFVSVAGHPALSPLPSREGADLGPAVR
                     VIGNGPFRAGGTIARQEEFHLERFDDHFAAEPAIRRLTFRPFEDADDAWPEFLAGDVD
                     IAPIPASLLAEAEGRYGEAG"
     gene            complement(881..1108)
                     /gene="secG"
                     /locus_tag="VG709_03805"
     CDS             complement(881..1108)
                     /gene="secG"
                     /locus_tag="VG709_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWC32230.1"
                     /translation="MLNITLIVIHVIASVLLVLFVLLHAGKGGGLSDMFGGGAGGLSS
                     GTAVERNLDRITVATALVFTVTTFLLAWRWD"
     gene            complement(1109..2167)
                     /locus_tag="VG709_03810"
     CDS             complement(1109..2167)
                     /locus_tag="VG709_03810"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWC32231.1"
                     /translation="MSAIDVDATRQLTAREGRVPLVRLVNVKKHFPITRGIVFQRKVG
                     SVHAVDGVDLEIYPGETVGLVGETGCGKSTTARLVTKLLDPTDGQIFFDGQDITRMRR
                     REMRPLRREIAMIFQDPYASLNPRKTVGSIIGEPFRIHRTVPKARVKAEVQQLMELVG
                     LNAEHYNRFPHEFSGGQRQRVGVARALALRPRLIVCDEPVSALDVSIQAQILNLLEDL
                     QEEFNLTYLFIAHDLSVVKHMSDRVSVMYLGKIVEVAPGETLYENPKHPYTGALLSAV
                     PVPDPRLATRKRRITLEGDVPSPIDPPSGCRFHPRCPRAQFPVCRDNEPELTPHHRGQ
                     VAACHFPLEDHSVIQSSG"
     gene            complement(2164..3177)
                     /locus_tag="VG709_03815"
     CDS             complement(2164..3177)
                     /locus_tag="VG709_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32232.1"
                     /translation="MAAPLLDVRDLKVHFPTDDGLVKAVDGVSFTIDEGETRGVVGES
                     GSGKSVTFLTILGLVDRKHARIEGEVLFRGEHLLEASEQTMRTIRGAKISMIFQDPMT
                     SLHPFYKVGQQIVEVIRAHDHVPKREAWSRAVEMLRLVGIPRPKERADQFPHEFSGGM
                     RQRAMIAMALALNPDLLIADEPTTALDVTVQAQILDLIGRLKDEFGASVVIITHDLGV
                     VAEHADDVMVMYAGRIAEFGTREDIYSRAHHPYTWGLLQSVTRLDQPKLERLKPIPGL
                     PPSLIFVPSGCPFHPRCPYAMDVCRVEVPELLPSDGHHTAACHLPLSEKERIFDEEVA
                     VKT"
     gene            complement(3177..4148)
                     /locus_tag="VG709_03820"
     CDS             complement(3177..4148)
                     /locus_tag="VG709_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC32233.1"
                     /translation="MGPYLIRRTLFLVLVLLIVSWLTFLIFLKLPPGDPARRAAGRRS
                     NERIIELARYNLGLDRPWYVQYGRFAKGLVPWPGFFLNEQVYFSYDNFVPVKEEIYKR
                     LPVTATLAVGAAAVWIVIGIPIGVASATKPRSVRDRAGMLFALFGVSAPVFWLGQLFL
                     FIFWFKLGIAPSSGLDIGAGLWESVLAGKFVMPWIVLSLTFAAFYARMVRGNLIETLS
                     EDYIRTARAKGLSERRVVFRHGLRAALTPIVTMFGLDLGILLGGAVITETVFNLPGLG
                     QYAISALRVADFPAVMGVTIFAAFFIVVFNLVVDVVYAFLDPRVRYS"
     gene            complement(4215..6056)
                     /locus_tag="VG709_03825"
     CDS             complement(4215..6056)
                     /locus_tag="VG709_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWC32234.1"
                     /translation="MRRHMRVLAVLGAFALTAAACGGGGGPGGAGGDQPDLQPGGTMR
                     LAMLSDVEDAYDPQKEYYQVSWGLFRCCMLRTLVSYPGVPTDEGGNELVPDLATDLPE
                     VSEDGLTWTFRLKEGIKFGPPFADQEIVADDFINAMEREADPDVGAGYPFYYSIIEGF
                     DEYSEGEADTISGMTAVDDKTLEINLTGPFADLGFRMAMPAAAPIPEGAAEGHEEDYG
                     RFLVPSGPYMFEGSDELDPDGGQPVSGYVPGQSVTLVRNPSWDPATDDIREAHIDRFE
                     IAIGGSEEDIFNKIDAGELDLHLDGVPPAQLIQAFQQNPEREDQVASNSADGTRYIAF
                     NLAAPPFDDIQVRKALNFALDKDGMRRIRGGEIVGDLAGHIIPPSLLEGGLEDYDPYA
                     SENGQGDIEAAKEAMSQSKYDTDEDGVCDAPECSGIIAVQDEAFPYSDQNALIQDNFS
                     QIGIELDIKSGERSNFMYARCQDPGANWAICLGPGWGKDYSDPTTFGEPLFGAAAIGP
                     DSCCNYSLVGASPELLQEFGYEVTEVPGVDDQIKQCDETPVGPERTACWVELDQELME
                     NVVPWIPYLFDNDVFVWSDRLQNYKYDQFSGQPALDHMALAGGGAES"
     gene            complement(6145..7137)
                     /locus_tag="VG709_03830"
     CDS             complement(6145..7137)
                     /locus_tag="VG709_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC32235.1"
                     /translation="MAQIAGEPTVGPEVVASGTEAASQGVVGRTPWQLFWRRFRRDKF
                     AIAGLVFLAIVAVVAFAAPLFARLTGHGPNDVFLREVTTEVGLPTGPSSEFWFGGTRA
                     GQDLFVRVIYGARTSLSVALVATGISLFIGVLLGLAAGYYRGKTDTVISRITDIVLSL
                     PILLLALGLVAACGLSREGCLGGLIRPGLLLVSYVIGLFSWPYIARIVRGQVLSLREK
                     EFIEAARSQGAGDFRIMLREVLPNVIAPIIVYTTLIIPNNILFEAALSFLGIGVPPTT
                     PSWGRMLADASVQGTYTVAPWTMVFPGIFLFMTTLAFNLVGDGLRDALDPRPAR"
     gene            complement(7245..8507)
                     /gene="apgM"
                     /locus_tag="VG709_03835"
     CDS             complement(7245..8507)
                     /gene="apgM"
                     /locus_tag="VG709_03835"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWC32236.1"
                     /translation="MTKLLYVCLDGLGDEPIPALDDRTPLEAAATPFLDELAARGRQG
                     LVHTVGRDIAPESDIAVFAILGYDPTEEHPGRGVLEAIGAGMDFRDGDLALRVNFATA
                     DGDRIVDRRVGRDLPSEDAHALADEVNRRLELPGASAELRATVEHRGALVIRADAGAL
                     SAQVGNTDPAYAKQGSLGVALATFEDRLAVAEPLDGTEDARLSADLTNAYVRDSARIL
                     DASDVNARRRREGRLPANLILTRDAGDHVPKLQPIRDRFGPSWGCFVEMPVERGIALM
                     LGMGAVDAPSPADDAERYAAWASLAAEALEGYDALYIHIKGPALPAHDGRAEEKRDIV
                     EVIDRAFFGEVLPRIDARTVFVVTADHATSCLRKAHTADPVPLVVSGAGVHPDGTSAF
                     GERAAASGAIGTIMGVEIVPSLVGLLSP"
     gene            complement(8504..9268)
                     /gene="tpiA"
                     /locus_tag="VG709_03840"
     CDS             complement(8504..9268)
                     /gene="tpiA"
                     /locus_tag="VG709_03840"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWC32237.1"
                     /translation="MRRAVVAANWKMHKTHLEAIQAVQKLSYLLDQEDTERVETVVCA
                     PFTALRSVQTLIDSDRLPFGLGAQDVYWEARGPFTGEISAPMLAALKVRYVIVGHSER
                     RQSFGETDANVARKVKAVADADMAPILCVGETAEQRDAGQTDDVVVRQVREGVSRMSA
                     DAASRAVIAYEPVWAIGTGRACDPGDAGAVIRTIRDTLETVFSTDVAEAVRVQYGGSV
                     KPGNAREFLAHPEIDGARVGGASLDPEELALIVKAG"
     gene            complement(9265..10455)
                     /locus_tag="VG709_03845"
     CDS             complement(9265..10455)
                     /locus_tag="VG709_03845"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWC32238.1"
                     /translation="MGRLRTIDDLGDLDGRCVLVRVDFNVPLDDGAVADDLRIRAALP
                     TLEDLRKRGAALVLASHLGRPKGKVVDELRLDAVAAALEDLGGFPVRKLDEVVGDAVT
                     DICGRLGGGDVAMLENLRFEPGEEENDDAFADALASLADAYVDDAFGAAHRAHASVVG
                     VAERLPGAAGLLMLKEVEVLSRLLEDAEEPFVAVLGGAKISDKLEVVRALLDRVDALL
                     IGGAMAFTFVVAQGGSVGDSLVEDDHVDHVRETLTEAERRGVLVLLPEDVVAAPEATA
                     DARKETVRPDDIPRGLKGLDVGPRTVEEFTRVIADARTVFWNGPMGVFELEPFALGTR
                     GIAQAVAGAEAFTVVGGGDSVAAIRRLGFDDAVDHLSTGGGASLEFLEGKTLPGVAVL
                     MEEP"
     gene            complement(10460..>10657)
                     /locus_tag="VG709_03850"
     CDS             complement(10460..>10657)
                     /locus_tag="VG709_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWC32239.1"
                     /translation="LKGYLRYTEDPIVSQDIVGDPHSCIFDADSTMAIGNLVKVLGWY
                     DNEWGYSNRLVELVELVGQRL"
BASE COUNT         1696 a         3681 c         3648 g         1632 t
ORIGIN      
        1 atcccggcct cgccgtagcg tccctcggcc tccgcgagga gcgacgccgg gatcggcgcg
       61 atgtcgacgt cgcccgcgag gaactccggc caggcgtcgt ccgcgtcctc gaaaggacgg
      121 aacgtgagcc gtctgatcgc cggctccgcc gcgaagtggt cgtcgaaccg ctcgagatgg
      181 aactcttcct ggcgcgcgat cgtgcccccg gcccggaaag gcccgttgcc gatcacgcgc
      241 accgccggcc cgaggtccgc gccctcccga gatggcagcg gcgacagcgc cgggtgtccg
      301 gccacagaga cgaaatcggg ccacgggctg ctgagccgga cctccagcgt cgtcgcatcg
      361 agggcacgga caccctcgag ctcacgcacc tggacgtcgc tctggtagct gtcgtagccg
      421 gcgacggact cgagcaggaa cgcgaacggg gctgcggccg ccggatccgc gagccggttc
      481 cacgcgaaga cgaaatcttc cgcctggacc ggcgacccgt catggaacgt ggcgccgcct
      541 cggaggtgga agacgaacgt ccggcctccg tcccgcacct cccagctctg cgccgcggac
      601 ggaacgagct cggtcgtctc gggctcgtac gcgacgagtc cgtcgaagac ctggtccgcg
      661 accacgatct cgtcgagggt ctcgagctcg acggggtcga tgctctcgac gtcctgggtc
      721 gcgatccgga gcgacggacg cgtcgggaac gacaggtcct ccccgccccg cgggtcgctc
      781 tcgactgggg gcttcgtgca ggcggcgagg acgaacgcgg cggcgaccgc gaccgcggag
      841 cgggcgacgc gagcggcgag gctgggcggg gcctctgccc ctagtcccac cgccaggcca
      901 gcaggaacgt cgtcaccgtg aagaccagcg cggtcgcgac ggtgatccgg tcgaggttgc
      961 gctccacggc cgtcccactc gagaggccgc cggcgccgcc cccgaacatg tccgagaggc
     1021 cgccgccctt ccccgcgtga agcagaacga acagcacgag gaggaccgac gcgatgacat
     1081 ggatgacgat cagcgtgatg ttgagcatct agcctgacga ctgtatcacg ctgtgatctt
     1141 ctaacgggaa gtggcacgcg gcgacctgtc cgcggtggtg cggcgtgagc tccggctcgt
     1201 tgtctcgaca gacggggaac tgcgcccgcg ggcaccgcgg atggaagcgg cagccggagg
     1261 gcggatcgat cggtgacggc acgtcccctt ccagggtgat ccggcgcttc cgtgtcgcga
     1321 gccgtggatc ggggacggga acggcggaga gcaaggctcc ggtatacggg tgtttcgggt
     1381 tctcgtacag cgtctcgccg ggtgcgacct cgacgatctt gccgagatac atcaccgaga
     1441 cgcggtcgct catgtgcttc acgaccgaga ggtcgtgtgc gatgaagagg tacgtgaggt
     1501 tgaactcctc ctgaaggtcc tcgagaaggt tgaggatctg cgcctggatc gagacgtcga
     1561 gcgcggacac cggctcgtcg cagacgatga ggcgcggccg gagcgcgagc gcacgagcga
     1621 ccccgacgcg ctgacgctgg ccgcccgaga actcgtgcgg gaaccggttg taatgctcgg
     1681 cgttgaggcc gaccagttcc atcagctgct ggacttccgc cttcactcgg gccttcggga
     1741 ccgtgcggtg gatccggaac ggctcgccga tgatcgaccc cacggtcttc cgcggattca
     1801 gcgacgcata cgggtcctgg aagatcatcg cgatctcgcg acgcagcggc cgcatctcgc
     1861 gacggcgcat cctcgtgatg tcctgcccgt cgaagaagat ctgtccatcg gtcgggtcga
     1921 ggagcttcgt gacgaggcga gcggtggtgg acttgccaca gccggtttcg ccaacgagac
     1981 cgaccgtctc gccgggatag atctcgaggt ctacgccgtc gacggcgtgg acgcttccga
     2041 ccttccgctg gaagacgatc cccctcgtga tcgggaagtg cttcttgacg ttgacgagcc
     2101 tcacgagcgg cacacgtccc tcacgcgccg tcagctgccg ggttgcgtcg acgtcgatcg
     2161 cgctcatgtc ttgaccgcca cctcctcgtc gaagatgcgc tccttctcgc tcagcggcag
     2221 atgacacgcg gccgtgtggt gtccgtcgga gggcaggagc tccgggacct ccacccgaca
     2281 cacgtccatc gcgtacgggc accgagggtg gaacggacac ccggacggca cgaagatcag
     2341 cgacggggga agccccggga tcggcttgag ccgttcgagc ttgggctggt cgagccgcgt
     2401 caccgactgg aggaggcccc aggtgtacgg gtggtgcgcg cgggagtaga tgtcctcgcg
     2461 cgtgccgaac tccgcgatcc gtcccgcgta catgaccatc acgtcgtcgg cgtgctcggc
     2521 gacgacgccg agatcgtggg tgatgatgac cacggacgct ccgaactcgt ccttcaagcg
     2581 gccgatcaga tcgaggatct gcgcctggac cgtcacgtcg agcgcggtcg tgggttcgtc
     2641 ggcgatcagc agatcggggt tcagcgcgag cgccatcgcg atcatcgccc gctgacgcat
     2701 gccgcccgag aattcgtgcg ggaactggtc cgcgcgctcc ttcggacgcg ggatcccgac
     2761 cagccgaagc atctcgaccg cacggctcca tgcctcgcgc ttcgggacgt ggtcgtgtgc
     2821 ccggatcacc tcgacgatct gctggcccac cttgtagaac gggtggaggg aggtcatcgg
     2881 gtcctggaag atcatgctga tcttcgctcc cctgatggtc cgcatcgtct gctcggacgc
     2941 ttcgaggagg tgctcgccgc ggaacagcac ctccccctcg atcctcgcgt gcttccggtc
     3001 gaccagcccc aagatcgtga ggaacgtcac gctcttcccc gacccggact cccccacgac
     3061 gcctcgcgtc tcgccctcgt cgatcgtgaa tgacacgccg tcgacggcct tcacgagccc
     3121 gtcgtcggtc gggaaatgga ccttcaggtc acggacgtcg aggagcggcg ctgccatcag
     3181 ctgtaccgga ccctcgggtc gaggaaggcg tagacgacgt cgacgacgag gttgaacacg
     3241 acgatgaaga acgccgcgaa gatcgtcact cccatcacgg ccgggaagtc cgcgacgcgc
     3301 agcgcggaga tcgcgtattg cccgaggccc gggaggttga acacggtctc ggtgatcacg
     3361 gcgccaccga ggaggatccc gagatcgagc ccgaacatcg tcacgatcgg tgtgagcgct
     3421 gcgcgcaggc cgtgccggaa cacgacccgg cgttcggaca gccccttcgc ccttgcggtc
     3481 cggatgtagt cctcgctgag cgtctcgatg aggttgcctc ggaccatccg cgcgtagaac
     3541 gccgcgaacg tgagagacag cacgatccac ggcatcacga acttcccggc gagaaccgat
     3601 tcccagaggc ccgcgccgat atcgagcccg ctcgaaggcg cgatgccgag cttgaaccag
     3661 aagatgaaca ggaagagctg tcccagccag aagaccggcg ccgacacgcc gaacagcgcg
     3721 aacagcatcc cggcccggtc acgcacggac ctcggtttcg tcgcggaggc gacgccgatc
     3781 gggatcccga tcacgatcca gaccgcagcg gcgccgaccg cgagggtcgc ggtgacgggg
     3841 agccgtttgt agatctcctc cttcaccggg acgaagttgt cgtacgagaa gtacacctgc
     3901 tcgttgagga agaaccccgg ccacggcacg aggcccttcg cgaagcggcc gtactggacg
     3961 taccacggtc gatcgagccc gaggttgtag cgggccagct cgatgatccg ttcgttcgat
     4021 cgtcggcccg ccgcccgccg cgcggggtcc cccggcggca gcttcaggaa gatcaggaac
     4081 gtgagccacg acacgatcag caggaccaat acgaggaaga gggtcctgcg gatcaggtat
     4141 gggcccactc gtcgctcctc gggaggaccg agggggcccc gcggagggcc ccctcgtatc
     4201 cgaccgcctc ggctctagct ctcggcgccg cccccggcca gcgccatgtg gtcgagcgcg
     4261 ggctggcccg agaactggtc gtacttgtag ttctgcagcc ggtccgacca cacgaacacg
     4321 tcgttgtcga agagatacgg gatccaaggg acgacgttct ccatgagctc ttggtcgagc
     4381 tcgacccagc acgccgtccg ttcggggccg acgggagtct cgtcgcactg cttgatctga
     4441 tcgtcgacgc ccggcacctc cgtgacctcg tacccgaact cctgcagcag ctcgggcgac
     4501 gcgccgacga gcgagtagtt gcagcacgag tcggggccga tcgctgccgc cccgaacagc
     4561 ggctcgccga aggttgtggg atcggagtag tccttgcccc agcccggccc gagacagatc
     4621 gcccagttgg cgccgggatc ctggcaccgc gcgtacatga agttgctgcg ctcgcccgac
     4681 ttgatgtcga gctcgatccc gatctgcgag aagttgtcct ggatcagcgc gttctggtcc
     4741 gagtagggaa acgcctcatc ttggaccgcg atgatcccgg agcactcggg ggcgtcgcag
     4801 accccgtctt cgtcggtgtc gtacttcgac tggctcatcg cctccttggc ggcctcgatg
     4861 tcgccctggc cgttctccga cgcgtagggg tcgtagtcct ccagcccgcc ctcgaggagg
     4921 ctcggcggga tgatgtgtcc ggcgaggtcc ccgacgatct ccccgccacg gatccggcgc
     4981 atgccgtcct tgtcgagcgc gaagttcagc gctttccgaa cctggatgtc gtcgaagggt
     5041 ggcgccgcga ggttgaacgc gatgtaccgc gtgccgtcgg cggagttcga cgcgacctgg
     5101 tcctcgcgct ccgggttctg ctggaacgcc tggatcagct gggcgggtgg gacgccgtcg
     5161 aggtggaggt ccagctcgcc ggcgtcgatc ttgttgaaga tgtcttcttc cgaaccgccg
     5221 atcgcgattt cgaagcggtc gatgtgcgcc tcgcggatgt cgtccgttgc cggatcccac
     5281 gaagggttcc gcacgagcgt gaccgactgg ccgggcacgt acccgctcac cggctggccg
     5341 ccgtccgggt cgagctcgtc gctgccctcg aacatgtacg gacccgacgg gacgaggaac
     5401 cgtccgtagt cctcttcgtg cccctcggcc gcaccctccg ggatcggcgc cgccgccggc
     5461 atcgccatgc ggaagccgag gtccgcgaac gggcccgtga ggttgatctc gagggtcttg
     5521 tcgtcgaccg cggtcatgcc gctgatcgtg tctgcctcac cctccgagta ctcgtcgaag
     5581 ccctcgatga tcgagtagta gaaggggtat cccgctccga catcgggatc agcttcgcgc
     5641 tccatcgcgt tgatgaagtc gtcggcgacg atctcctggt ccgcgaacgg aggcccgaac
     5701 ttgatgccct ccttcaggcg gaaggtccag gtcaggccgt cctccgagac ctctgggagg
     5761 tcggtcgcga ggtcgggaac gagctcgttg ccgccctcgt ccgtcggcac gccggggtag
     5821 gagaccagcg tacggagcat gcaacagcgg aacaggcccc acgagacctg gtagtactcc
     5881 ttctgcgggt cgtacgcgtc ttcgacgtcg gacagcatcg cgagacgcat ggtgccgcca
     5941 ggctgcaggt ccggctgatc gccgcccgcg ccgccgggac cgccgccacc accacacgcg
     6001 gccgccgtca gcgcgaacgc gcccaggacc gcgagcaccc tcatgtgccg cctcatgtga
     6061 tcccccctct agagatcgct tcggtgcgac gaccctacca ctgcgggcga cgttgcggat
     6121 tcgtccttcg tgcctccctt ccgctcatcg agccggcctg ggatcgagcg cgtcccgaag
     6181 cccgtcgccg acgaggttga acgccagcgt cgtcatgaac aggaagatgc cggggaagac
     6241 catcgtccac ggagccaccg tgtacgtgcc ctgtacggag gcgtccgcga gcatccgtcc
     6301 ccacgacggc gtcgtcggcg ggaccccgat cccgaggaac gacaacgccg cctcgaacag
     6361 gatgttgttc gggatgatca gcgtggtgta gacgatgatc ggcgcgatca cgttcgggag
     6421 aacctcacgc agcatgatcc tgaagtcgcc ggcaccttgc gatcgcgccg cctcgatgaa
     6481 ctccttctct cgaagcgaca ggacctgccc ccggacgatc cgcgcgatgt acggccagct
     6541 gaagagaccg atcacgtagc tcaccagcaa gagacccgga cggatcagcc cgccgagaca
     6601 gccctccctc gacaagccgc acgccgccac gaggccgagc gcgagaagca ggatcggaag
     6661 cgacagcacg atgtccgtga tccgcgagat gacggtgtcg gtcttaccgc ggtagtaacc
     6721 ggccgccagc ccgagcagga cgccgatgaa gagcgagatg ccggtggcga cgagcgcgac
     6781 ggagagcgac gtccgcgccc catagatcac gcgtacgaag aggtcctgac cggcacgcgt
     6841 tccgccgaac cagaactccg agctcggacc cgtcggaagc ccgacctccg tcgtgacctc
     6901 gcgcaggaag acgtcgttgg gtccgtggcc cgtgagtcgc gcgaagagcg gcgccgcaaa
     6961 cgcgacgacg gccacgatcg cgaggaagac gaggccggcg atcgcgaact tgtcgcggcg
     7021 gaaacggcgc cagaagagct gccaaggcgt ccgtccgacg acgccctgac tcgctgcctc
     7081 cgtgcccgac gcgacgacct cgggccccac ggtgggttcg cctgcgatct gcgccactca
     7141 gcgtcctcgg atcatcggcc ctgcgaatgg tattccgggc gcgcaggatc tgcttgagct
     7201 cttgagacgt accgcgtcag gactcaccca gggcggtcga aggatcaggg cgacaggaga
     7261 ccgaccagcg acggaacgat ctcgaccccc atgatcgtgc cgatcgcgcc gctcgcggcg
     7321 gctcgttcgc cgaacgcgga tgtgccgtcg gggtggaccc cagcgccgga cacgacgagc
     7381 ggaaccgggt ccgcggtgtg ggcttttcgg aggcatgagg tcgcgtggtc ggccgtcacg
     7441 acaaagacgg tgcgcgcgtc gatgcgtgga agcacttcgc cgaagaacgc gcggtcgatc
     7501 acctcgacga tgtcgcgctt ctcctctgcg cggccgtcgt gagccggcag ggcggggccc
     7561 ttgatgtgga tgtagagggc gtcgtagccc tcgagcgcct cggcggcgag cgatgcccac
     7621 gccgcatagc gctcggcgtc gtccgcgggg ctcggggcgt cgaccgcgcc catgccgagc
     7681 atcagcgcga tcccacgttc gaccggcatc tcgacgaagc atccccacga cggaccgaac
     7741 cggtcgcgga tcggctggag cttcggcacg tgatcgccgg catcgcgcgt gaggatcagg
     7801 ttcgcgggga ggcggccctc gcggcggcgg cgcgcgttga cgtccgaggc gtcgaggatc
     7861 cgcgccgagt cacggacgta cgcgttggtg agatcggcgg agaggcgggc atcctccgtg
     7921 ccgtcgagcg gctcggcgac cgcgagccgg tcctcgaacg tcgcgagcgc gacgccgagg
     7981 ctcccctgct tcgcgtaggc cggatcggtg ttgccgacct gcgccgagag cgcgcccgcg
     8041 tccgcgcgga tcacgagcgc gccgcgatgc tcgacggtcg cccgcagctc cgcggaggcg
     8101 ccgggaagct cgaggcgccg gttcacctcg tccgcgagcg cgtgcgcgtc ctcggagggc
     8161 agatcgcgcc cgacgcggcg gtcgacgatg cggtcgccgt ccgccgtcgc gaagttcacg
     8221 cgcaacgcga ggtcgccgtc gcggaaatcc atgccggcgc cgatcgcctc gaggacgccg
     8281 cgcccggggt gctcctcggt cgggtcgtac ccgaggatcg cgaacacggc gatgtcggac
     8341 tcgggggcga tgtcgcgtcc gacggtgtgc acgagcccct ggcgtccgcg tgcggcgagc
     8401 tcgtcgagga acggcgtcgc ggccgcctcg agcggcgtcc ggtcgtccag ggccgggatc
     8461 ggctcgtcgc cgaggccgtc gaggcagacg tagaggagct tcgtcatccc gccttgacga
     8521 tcagagcgag ctcctccggg tcgaggctcg cgccgccgac gcgcgcgccg tcgatctcgg
     8581 ggtgcgcgag gaactctcgc gcgttccccg gcttgacgct cccgccgtac tggacgcgga
     8641 ccgcctcggc gacgtcggtc gagaacacgg tctcgagcgt gtcgcggatc gtgcggatga
     8701 cggcgccggc gtcgcccggg tcgcacgccc gaccggtccc gatcgcccac accggctcgt
     8761 aggcgatcac ggcgcgcgac gcggcatcgg ccgacatgcg cgagaccccc tccctgacct
     8821 gtctgacgac gacgtcgtcg gtctgtccgg cgtcccgctg ctccgcggtc tcgccgacgc
     8881 agaggatcgg cgccatgtcc gcgtcggcga ccgccttgac cttgcgggcg acgttcgcgt
     8941 cggtctcgcc gaagctttgc cggcgctccg agtggccgac gatcacgtac cgcaccttga
     9001 gcgcagccag catcggcgcc gagatctctc cggtgaacgg gccccgtgcc tcccagtaga
     9061 cgtcctgcgc tccgaggccg aacggcaggc ggtcgctgtc gatcagcgtc tggacgctcc
     9121 tgagcgctgt gaacggcgcg cagacgaccg tctcgacgcg ctcggtgtcc tcctggtcca
     9181 gcaggtacga gagcttctgg accgcttgga tcgcctcgag gtgcgtcttg tgcatcttcc
     9241 agttcgccgc gacgacagcg cgcctcatgg ctcctccatc aggaccgcga cccccggaag
     9301 cgtcttaccc tcgaggaact cgagcgaggc gccgcccccg gtcgagaggt ggtcgacggc
     9361 gtcgtcgaag ccgagccgtc ggatcgccgc gacgctgtcg ccgccgccga cgaccgtgaa
     9421 cgcctccgca cctgcgacgg cctgcgcgat gccgcgcgtc ccgagcgcga acggctcgag
     9481 ctcgaagacc cccatcgggc cgttccagaa caccgtccgc gcgtcggcga tcacgcgggt
     9541 gaactcctcg acggtccgag ggccgacgtc gaggcccttc agtccccgcg ggatgtcgtc
     9601 ggggcggacc gtctccttgc gcgcgtcggc ggtcgcctcc ggcgccgcga cgacgtcctc
     9661 gggaagtagc actaggacgc ctcgtctctc cgcctcggtg agcgtctcgc gaacgtggtc
     9721 gacgtggtcg tcctctacca gcgagtcgcc gacgcttccg ccctgcgcca cgacgaacgt
     9781 gaacgccatc gcgccgccga tgaggagcgc gtcgacgcgg tcgagcagcg cgcggacgac
     9841 ctcgagcttg tcggagatct tcgcgccgcc gaggaccgcg acgaagggct cctcggcgtc
     9901 ctcgaggaga cgcgagagca cctcgacctc cttcagcatg aggagcccgg cggcgcccgg
     9961 gagacgttcc gccacaccga cgacggaggc gtgcgcgcga tgagcggccc cgaacgcgtc
    10021 gtcgacgtag gcgtcggcga gcgaggcgag ggcgtcggcg aacgcgtcat cgttctcctc
    10081 ctccccgggc tcgaagcgga ggttctcgag catcgcgacg tcgccgccgc cgagccggcc
    10141 gcagatgtcg gtcaccgcgt cccccacgac ctcgtcgagc tttcggaccg ggaagccccc
    10201 gaggtcctcg agcgccgcgg cgacggcgtc gagccgaagc tcgtcgacga ctttcccctt
    10261 cggtcgcccg aggtggctcg cgaggacgag cgccgcgccg cgcttccgca ggtcctcgag
    10321 cgtcggcagc gccgcgcgga tccgaaggtc gtcggcgacc gccccgtcgt cgagcggcac
    10381 gttgaagtcg acgcggacga gcacgcagcg accgtcgagg tcgcccaggt cgtcgatcgt
    10441 ccgcagccgg cccactccgc tagaggcgct ggccgacgag ctcgacgagc tcgacgagcc
    10501 ggttcgagta cccccactcg ttgtcgtacc agccgagcac cttgacgagg ttcccgatcg
    10561 ccatcgtcga gtcggcgtcg aagatgcagg agtgcggatc gccgacgatg tcctgcgaca
    10621 cgatcgggtc ctcggtgtac cggaggtagc ccttcag
//
LOCUS       DASZOB010000232         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_106354, whole genome shotgun sequence.
ACCESSION   DASZOB010000232 DASZOB010000000
VERSION     DASZOB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106354"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1535)
                     /locus_tag="VG709_03855"
     CDS             complement(27..1535)
                     /locus_tag="VG709_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HWC32240.1"
                     /translation="MIERTLVDYHERTKHSYESIRREGGGLDWSNEPSPFKEYLDLDA
                     IALPPPEGTGVPAHDAITASTDVKSDAPLTTDALSYVLFHGAGVTRIFRGSFGELRFR
                     AYASAGALYPNEVYLVSGGTEGLDAGVYHYGPRDHDLRLLREGDHRGALGLAGAQPGQ
                     ASLVVTGLPWRTAWKYGTRGFRHLYWDAGMIVANVLGSGAARGVRATVLLGFVDEAVA
                     RMIGVDGETEFPLCIVALGDADAPPHNEVTEMISHRVAPVSRRPRRDPGIERAHAATM
                     LRTEAEVEAVRAAVREAHGDGERRVERVEPFPRERLSRDGFERVVRRRGSSRSLAREA
                     FPAGELAAVLDLALATFPSDWPSGAVRVFLVASALEGVPPGAYRYDGGGSFAEIRAGD
                     LRRKAGFVCLEQRLAADGAAALFLMGDVESELATLGSRGYVAAQLEAAIVAGRIYLGA
                     YAQCLGASGITFYDDEVRQLFETSAEPMLAVVVGPEGQRESIIRCRQRLTAG"
     gene            complement(1546..2052)
                     /locus_tag="VG709_03860"
     CDS             complement(1546..2052)
                     /locus_tag="VG709_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HWC32241.1"
                     /translation="MSDESPRELIVVNDNEGPEPSGEAVLQPGKLLRIASMTRELLEE
                     VRQTTLDEAGRARLQHVHQSTVDELDEVLSEDLREELRELAPPLEGTASAAEIRVAQA
                     QLLGWLEGLFHGIQAALWAQQVQARSALDEMRRRGLPPGAPHAHGQPAGRPDAGGREP
                     NTPPGQYL"
     gene            complement(2091..2246)
                     /locus_tag="VG709_03865"
     CDS             complement(2091..2246)
                     /locus_tag="VG709_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32242.1"
                     /translation="MEGVINPLAVRDPWRCSNCGNEWLVYDVDDRDGAVTCPRCESTD
                     VAPIRGS"
     gene            2328..2939
                     /locus_tag="VG709_03870"
     CDS             2328..2939
                     /locus_tag="VG709_03870"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HWC32243.1"
                     /translation="MILLGAIVVAAMLLAFGLGGRLRNLADPDVRVRWWPILPLSLAL
                     QVAPIPQVGTGVGRYLPVVAVLVSYLILGVVLVANIRLRGFVLILLGSLLNVVVIAAN
                     QGMPVSRAAIVESENISLLEGLPTERGHAYHAATEGDVLLPLADVIPVRQPFGVVVSV
                     GDVFTYSGTALFLAAAMLGRPRRRSPRRDPVSREPATTSGTQP"
BASE COUNT          411 a         1036 c         1054 g          465 t
ORIGIN      
        1 gaggtgaacc gctcgcatcc gagggattat ccggcggtga ggcgctggcg acagcggatg
       61 atcgactccc gctgcccctc cggcccgacg acgaccgcga gcatcggctc ggcggaggtc
      121 tcgaacagct gacgtacctc gtcgtcgtag aacgtgatgc cgctcgcgcc gaggcactgc
      181 gcgtaggcgc cgaggtaaat ccggccggcg acgatggccg cctcgagctg cgccgcgacg
      241 tacccgcgcg atccgagcgt cgcgagctcc gactcgacgt cgcccatcag gaacagcgcg
      301 gcggcgccgt cggcggcgag gcgctgctcg aggcacacga atccggcctt gcggcgtaga
      361 tcgccggcgc ggatctcggc gaacgagcca ccgccgtcgt accggtaggc gccgggcggc
      421 acgccctcga gcgcggaggc aacgaggaag acgcgaacgg ccccggacgg ccagtccgat
      481 gggaacgtgg cgagcgcgag gtcgaggacc gccgcgagct cgccggccgg gaaggcctcg
      541 cgcgcgagac tgcggctcga tccgcgccgc cgcaccacac gttcgaagcc gtcgcgcgac
      601 aaccgctcgc gcgggaaggg ctcgacgcgc tcgacgcgtc gttccccgtc gccgtgcgcc
      661 tcgcgcacgg ccgcgcggac cgcctcgacc tccgcctccg tgcgcagcat ggtggcggcg
      721 tgcgctcgtt cgatcccggg gtcccggcgc gggcgcctcg agacgggcgc gacgcgatgc
      781 gagatcatct ccgtgacttc gttgtgcggt ggcgcgtccg cgtcgccgag cgcgacgatg
      841 cagagcggga actcggtctc gccgtcgacg ccgatcatgc gcgcgacggc ctcgtcgacg
      901 aaaccgagga gcacggtcgc gcgcacgccg cgcgccgctc ctgatcccag cacgttcgcg
      961 acaatcatgc cggcgtccca gtagagatgc cggaacccgc gcgtgccgta cttccacgcg
     1021 gtcctccacg ggaggccggt cacgacgaga gacgcctgcc ccggttgcgc gccggcgagc
     1081 cccagcgccc cgcgatggtc gccctcgcgg agcaaccgta ggtcgtggtc ccgtggcccg
     1141 tagtggtaga cgcccgcgtc gagcccttcg gtgccgccag acacgaggta gacctcgttt
     1201 gggtacagcg cgccggcgct cgcatacgcg cggaacctga gctcgccgaa cgatccgcgg
     1261 aagatccgcg tcacgccggc gccgtggaag agcacgtaag agagcgcgtc ggtggtgagc
     1321 ggcgcgtcgg acttcacatc ggtcgaagcc gtgatcgcgt cgtgcgccgg gacgcccgtc
     1381 ccctccggcg gagggagcgc gatggcgtcg aggtcgaggt actccttaaa gggcgacggc
     1441 tcgttcgacc agtcgaggcc gccgccctcg cgccggatgc tctcgtacga atgcttggtg
     1501 cgctcgtggt aatcgacgag cgttctctcg atcacacgag ggacgctaga ggtactgccc
     1561 cggcggtgtg ttcggctccc gtccgcccgc gtccggccgg cctgccggtt gcccgtgggc
     1621 gtgcggcgcc ccaggaggca gcccgcggcg cctcatctcg tcgagagcgg accgcgcctg
     1681 gacctgctgc gcccacagcg cggcctggat tccgtggaag aggccctcga gccatccgag
     1741 gagctgcgcc tgtgcgaccc ggatctcggc cgcgctcgcc gttccttcga gcggcggggc
     1801 gagctcacgg agctcctcac ggagatcctc cgacagcacc tcgtcgagct cgtcgaccgt
     1861 gctctgatgg acatgctgca gtcgcgcccg ccctgcttcg tcgagcgtcg tctgccgtac
     1921 ctcttcgagg agctcgcgcg tcatcgacgc gatccggagc agcttcccgg gctggaggac
     1981 ggcctcgccg gacggctcgg gcccttcgtt gtcgttgacg acgatcagct cacgcggaga
     2041 ttcgtcggac atcctcggag ttttccccct cgcgacgcga ggaaacgagg tcaggagccg
     2101 cggatcggcg cgacgtcggt gctctcgcag cgcgggcacg tcaccgcgcc gtcgcgatcg
     2161 tcgacgtcgt acacgagcca ctcgttgccg cagttcgagc accgccaggg gtcgcggacg
     2221 gcgagtggat tgatgacacc ttccaacgcg ctctcctttg agcgaggctt ttcagcgtac
     2281 caggtcgtga cgggtggatc gctggaggag ccccctaaga tgcccgagtg atcctgcttg
     2341 gggcgatcgt cgtcgccgcg atgctcctcg cgttcgggtt gggcggacgg ctccggaacc
     2401 tcgccgatcc cgatgttcgg gtccgatggt ggccgatcct cccgctctcg ctcgcgcttc
     2461 aggtcgctcc gatcccacag gtcggaactg gagtggggcg gtacctgccg gtggtggcgg
     2521 tgctcgtctc ctacctgatc cttggcgtgg tgctcgtcgc caacatccgg ctgcgaggct
     2581 tcgtgctgat cctcctcggg agcctactga acgtcgtcgt gatcgcggcg aaccagggga
     2641 tgccggtttc gcgcgccgcg atcgtcgagt cggagaacat ctcgctgctc gaggggcttc
     2701 cgacggagcg cgggcacgcg taccacgccg ccacggaggg tgacgtgctg cttcctctgg
     2761 cagacgtcat tccggtccgg cagccgttcg gagtcgtggt gagcgtcggc gacgtgttta
     2821 cctacagtgg gacggcgctg ttcctggccg cggctatgct cgggcgccct cgtcgacgga
     2881 gcccgcgtcg ggacccggtg tcgagagagc cagccacgac gtcgggaact cagccgtgac
     2941 gacggtgccg cggtcggttg cgctgt
//
LOCUS       DASZOB010000233         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_185222, whole genome shotgun sequence.
ACCESSION   DASZOB010000233 DASZOB010000000
VERSION     DASZOB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185222"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VG709_03875"
     CDS             complement(<1..414)
                     /locus_tag="VG709_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HWC32244.1"
                     /translation="MDREPVPQERLVRSTAVMAVGTTLSRVTGFLRIAAMAFAFGVAE
                     SRLPDAYNVANNLPNIVYELVLGGVLTSVLVPVFVERLTGRGKEDAWEAGHAVMTVAF
                     VLLGVLTIVGILAAPWIVKLYTFRGPGGAERVELHD"
     gene            complement(420..2441)
                     /locus_tag="VG709_03880"
     CDS             complement(420..2441)
                     /locus_tag="VG709_03880"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HWC32245.1"
                     /translation="MRRLLAAAMLAPSLAVVQLAPAAAQEEEEEEPAVRLVLEDQTTT
                     ASANRPFTMQVRIVNDGAETWSDLSLTFWLYPAARSRSEYEQALDVDLPTPLKVETLP
                     LGQRRLQPGEEVSVRLEPRIGVLEDLDENALYPLRVQLSSGLEPIAVLRSAVVYLVKP
                     PLIGLNVALVFVLDAPLVLGPRGVFADDTLEEALARGGHLRRVVDVLRDVPVRTTLVV
                     SPLLLQQLQAMVAGYEVVEVGGGTRAVPPDAPGARRAQRALDGIAEIARRPETQLVAL
                     PYASPSLPALVSAGMNDDLESQLARGRAVVEARLGEEPAETSLWPPASAVTEPALDAL
                     AAEGIENLLVDADTLPPPAGSKFTRSAIARLETDGGSSFRVLTPDPLIDARLSQGEVD
                     ARIRAQWVLGELASIYFEHPGRDRGVALVFAENDEPPDALLRRLLSVLRSATRAPWMR
                     AVTASRLLEPSLEPPPDRRTLDEPEHVGRFPAAFVEAVGEARDAIAQLASIGGASETI
                     DRLRELVLLSQSRYLLGDDERRIGYLDRVQEVVSEEFAKIQTPEPGVVTLTSLHGEVP
                     VTIRNLAEREVEVQITLQSSRLEFLEGSTHTVTLSPPAQRFEFPVRAETTGRFGVHVE
                     VRTPLGAEIAETDIGVRSTAYNRVALVVTIGAALFLLGLWARRFLPRRS"
     gene            complement(2465..>2740)
                     /locus_tag="VG709_03885"
     CDS             complement(2465..>2740)
                     /locus_tag="VG709_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWC32246.1"
                     /translation="DAAAREVREETGYAGAVRAPLGDVSYWYVWQGTRVRKTVHFFLM
                     DSTADGPGERDHEMEEVAWFPLDEAQDVAGFDSEKDVLQRAAEAVRR"
BASE COUNT          411 a         1008 c          949 g          372 t
ORIGIN      
        1 atcgtgaagc tcaacccgtt ccgcgccccc ggggccgcgg aacgtgtaga gcttcacgat
       61 ccatggcgcc gccaggatcc ccacgatcgt gaggacgccg agcagcacga aggcgaccgt
      121 catgacggcg tgcccggcct cccacgcatc ctccttgcct cgaccggtga ggcgctcgac
      181 gaacaccggc accaggaccg aggtcagcac gccgccgagc acgagctcgt agacgatgtt
      241 cggcaggttg ttcgcgacgt tgtacgcgtc ggggagtcgg ctctccgcaa cgccgaaggc
      301 gaacgccatc gcggcgatgc gcaggaaccc ggtgacccgg gacagcgtcg tcccgacggc
      361 catcacggcg gtgctgcgga cgagccgctc ctgcgggacc ggctcgcgat ccacgtcgtc
      421 tagctccgcc gtgggaggaa ccgccgggcc cagagcccga ggaggaacag cgccgccccg
      481 atcgtcacga cgagcgcgac gcggttgtag gcggtggagc gcacgccgat gtcggtctcc
      541 gcgatctccg cgcccagcgg cgtcctcacc tcgacgtgga ccccgaagcg ccccgtcgtc
      601 tcggcccgca cggggaactc gaagcgctgc gcggggggcg agagcgtcac ggtgtgcgtg
      661 cttccctcga ggaactcgag gcgcgacgac tggagcgtga tctggacctc gacctcccgc
      721 tccgcgaggt tgcggatcgt caccggcacc tcaccgtgaa gcgaggtgag cgtcacgacg
      781 ccgggctccg gcgtctggat cttcgcgaac tcctcggaca cgacctcttg gacgcggtcg
      841 aggtaaccga tccggcgctc gtcgtccccg agcaggtatc ggctctgcga gagaagcacg
      901 agctcgcgga ggcggtcgat ggtctcagag gcgcccccga tcgacgcgag ctgcgcgatc
      961 gcatctctcg cctcgccgac cgcctcgacg aacgccgccg ggaacctccc gacgtgctcc
     1021 ggttcgtcga gcgtgcgccg gtcgggcggc ggttccaggc tcggctcgag cagccggctc
     1081 gccgtcaccg cgcgcatcca cggcgcccgc gtcgccgagc ggaggaccga cagaagccgg
     1141 cggaggagcg cgtccggcgg ctcgtcgttc tcggcgaaca ccagcgccac gccccgatcc
     1201 cgtccggggt gctcgaagta gatcgatgcg agctcgccga ggacccactg cgcgcggatc
     1261 cgcgcgtcga cctcgccctg cgacagacga gcgtcgatca gcggatcggg ggtgagcacg
     1321 cggaacgagg atccgccgtc cgtctctagg cgagcgatcg cggaccgcgt gaacttcgac
     1381 cccgccggag gcggtagcgt gtccgcgtcc acgagcaggt tctcgatgcc ttcggcggcg
     1441 agcgcgtcga gcgccggctc ggtgaccgca gacgccggcg gccacagcga cgtctcggcg
     1501 ggctcctcgc cgagccgggc ctcgacaacg gcgcgtcccc gtgccagctg tgactcgagg
     1561 tcgtcgttca tgcctgcgct cacgagcgcc gggagcgacg gcgaggcgta ggggagcgcg
     1621 acgagctgcg tctccggccg acgtgcgatc tcggcgatgc cgtcgagcgc acgctgcgct
     1681 cgcctcgcac ccggggcgtc cggaggcacc gcccgggtgc ctcctcccac ctccaccacc
     1741 tcgtacccgg cgaccatcgc ctgcagctgt tggaggagca gcggcgacac gaccagcgtc
     1801 gtccgcaccg gcacgtcgcg cagcacgtcg acgacccggc ggaggtgccc gcctcgcgcg
     1861 agcgcctcct cgagcgtgtc gtccgcgaag acgccgcgcg ggcctaacac gagcggagcg
     1921 tcgaggacga atacgagcgc gacgttgagg ccgatcagtg gcggcttcac gagatagacg
     1981 acggcgctcc ggagcaccgc gatcggctcg aggcccgacg agagctggac gcgcagcggg
     2041 tagagggcgt tctcgtcgag gtcctctagg acgccgatcc gcggctcgag ccggaccgac
     2101 acctcctccc ccggctggag cctgcgctgg ccgagcggga gcgtctcaac cttgagcggc
     2161 gtcggcaggt cgacgtcgag cgcctgctcg tactcggatc gtgacctcgc cgccggatac
     2221 agccagaacg tcagcgagag gtcgctccac gtctcggcgc cgtcgttcac gatccggacc
     2281 tgcatcgtga acggacggtt cgcggaggcg gtcgtcgtct ggtcctcgag caccagccgg
     2341 accgccggct cctcctcttc ctcctcctgc gcggcggcgg gagcgagctg gacgaccgcg
     2401 agcgacggcg cgagcatggc ggccgccagg agacgtctca ccgacgcgcc attgtagaag
     2461 gagctcatcg ccggaccgcc tcggcggcgc gctggaggac gtccttctcg gagtcgaacc
     2521 cggccacatc ttgcgcctcg tcgagcggga accacgcgac ctcctccatc tcgtggtcgc
     2581 gctcaccggg cccgtccgcc gtcgagtcca tcaggaagaa gtggacggtc ttcctcacgc
     2641 gcgtcccctg ccagacgtac cagtacgaga catcgccgag cggcgctctc accgcgcccg
     2701 cgtacccggt ctcctcgcgc acctcgcgcg ccgccgcgtc
//
LOCUS       DASZOB010000234         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_32746, whole genome shotgun sequence.
ACCESSION   DASZOB010000234 DASZOB010000000
VERSION     DASZOB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32746"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VG709_03890"
     CDS             <1..586
                     /locus_tag="VG709_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32247.1"
                     /translation="AYVAGRYGLGRVVGIKQPPQQSAVRDGDGIDLQRTIDGHDPGGD
                     GTVPRPSATPLELGDDEGAAFSAERHASLQNDDHVLLQLTGALTGNTIRWDRFRGVLP
                     MTDVSLDVDDVYADSEPVVVRARPERDPDGELIAVAVDVETDEERARQPLVARDDGWY
                     DAELGPLPEGVYRVTAFGEGTVEPVSDLVSVLGE"
     gene            597..>1575
                     /locus_tag="VG709_03895"
     CDS             597..>1575
                     /locus_tag="VG709_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWC32248.1"
                     /translation="MNWAVVIGVDDYGRDELSLSAAVADAESFRDWVVAEDGGGVPEE
                     NVRVLLARRADDSAASDADRVPTKDNIVTAINEIVTASGGTGERLYFFFAGHGITARV
                     ANRDESALVTPGFDELHPDHSLAVRSLTEFLETTQFEDQFFFIDACRDVPWRNREFEI
                     GRWPIPRRRDPGKPPVQQFILHATSPGLTALEVGWPGEAVGAFSEVLMAGLREGAGRA
                     KAWSWERGCYEVRWENLATYVKAAMEERKHPTRPSAEAPPGGWPIQIPQDAGTRGVAG
                     RDRDALLVSFPRARFRPLRLTVELHADPRHDEAEVSVLDAVGEPVASALK"
BASE COUNT          223 a          567 c          575 g          210 t
ORIGIN      
        1 ggcatacgtc gccggtcgct acgggctcgg acgcgtcgtg ggcatcaagc agccgccgca
       61 gcagtcggca gtccgcgacg gcgacgggat cgacctccag cggacgatcg acggccacga
      121 cccgggcggc gacggaaccg ttccgcggcc gtccgcgacg ccgctcgagc tcggcgacga
      181 cgagggcgcg gcgttctcgg ccgagcgcca cgcgtcgctt cagaacgacg accacgtcct
      241 gctccagctc accggcgccc tcaccggcaa cacgatccgg tgggaccgct tccggggcgt
      301 cctgcccatg accgacgtct cgctcgacgt ggacgacgtc tacgccgact ccgagcccgt
      361 cgtcgtgcgc gcgcgcccgg agcgggaccc ggacggtgag ctgattgccg tcgccgtcga
      421 cgtcgagacg gacgaggagc gcgcgcggca gcccctcgtc gcgcgtgacg acggctggta
      481 cgacgccgag ctcgggcctc tgccggaggg cgtgtaccgc gtcaccgcgt tcggcgaggg
      541 caccgtcgaa ccggtgagcg acctcgtgag cgtcctcggc gagtaacgtc gggcccgtga
      601 actgggccgt cgtcatcggc gtcgacgact acgggcgcga cgagctgagc ctctccgccg
      661 ccgtggcgga cgccgagagc ttccgcgact gggtcgtcgc cgaggacggc ggaggcgtcc
      721 ccgaggagaa cgtgcgcgtg ctgctcgcgc ggcgcgccga cgattccgcc gcgagcgacg
      781 cggaccgcgt gccgacgaag gacaacatcg tgaccgcgat caacgagatc gtcacggcga
      841 gcggcgggac gggcgagcgc ctgtacttct tcttcgccgg ccacgggatc acggcgcggg
      901 tggcgaaccg cgacgagagc gccctcgtca cgccgggctt cgacgagctc catcccgacc
      961 attcgctcgc ggtccgctcg ctgacggagt ttctcgagac gacgcagttc gaggaccagt
     1021 tcttcttcat cgacgcctgc agggacgtgc cgtggcggaa ccgcgagttc gagatcggtc
     1081 ggtggccgat cccgcgccgg cgcgacccgg ggaagccgcc ggtgcagcag ttcatcctcc
     1141 acgccacctc tccgggcctc accgctctcg aggtcgggtg gcccggcgag gccgtcggag
     1201 cgttcagcga ggtgctcatg gctggtctgc gagaaggcgc gggtcgcgcg aaggcctggt
     1261 cgtgggagcg cggttgctac gaggtccgct gggagaacct cgccacgtac gtgaaggccg
     1321 cgatggagga gcggaagcac ccgacgaggc cgtcggccga ggcgccgccc ggcgggtggc
     1381 cgatccagat tccgcaggac gccggaaccc gcggcgtcgc cggccgcgat cgcgacgccc
     1441 tcctcgtctc gtttccgcgc gcccgcttcc gcccgcttcg cctgacggtc gagctccacg
     1501 ccgaccctcg ccacgacgag gcggaggtca gcgtgctcga cgccgtcgga gagcctgtcg
     1561 cgagcgcgct caagg
//
LOCUS       DASZOB010000235         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_106412, whole genome shotgun sequence.
ACCESSION   DASZOB010000235 DASZOB010000000
VERSION     DASZOB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106412"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VG709_03900"
     CDS             complement(<1..996)
                     /locus_tag="VG709_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWC32249.1"
                     /translation="MTATGDERGSDDASPAGGEARWPPVTYWMRVAAGVLVVLLLFRA
                     LAAIQNVVVLLLISLVLALGLQPAIDVLTRRGMRRGWAVAIIYTTTLVVAATFLALIV
                     PVIVREIGNLIEEAPEYLERAQEGNGFIAQLDERFDLRGKLRSLGEDVPGTALGLVRS
                     FAALVFNTLTVAILTAIFAVRLPDLRRGVTRLLRREHREDFEQILGASTQRVGGYVIG
                     NIIVSLIAGVVSFVALALLGVPYPAALAFWVAIADLIPTIGATLGAVVAVAVAAFAGP
                     VQLIGSIAFFAVYQQIENYVIAPRVMKRAIDMSPGAVIIAVLVGGSLAGFVGGLLA"
     gene            complement(1063..>1657)
                     /locus_tag="VG709_03905"
     CDS             complement(1063..>1657)
                     /locus_tag="VG709_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWC32250.1"
                     /translation="AVWGLGPIGQMCVRIARRRGAGRVIGVDRLPERLELAARHGAEV
                     IDLEHVPDGPAAVEDLTEGRGADSVIDAVGMEADGATIDHVLQTLKVQPDRLHALHQS
                     MSAVRRGGTLSVVGVYVGWMHAFPLGALFDRQITVRMGQANVRRWVDDLLPLLGDEDP
                     LGTADLTTHRVPLDAAPEAYEMLQRKRDGAIKVVLEP"
BASE COUNT          263 a          598 c          593 g          203 t
ORIGIN      
        1 tgcgaggagg ccgccgacga accccgcgag gcttccgccg accaggacgg cgatgatcac
       61 cgctccgggc gacatgtcga tcgcgcgctt catgacccgc ggcgcgatca cgtagttctc
      121 gatctgctgg tagacggcga agaaggcgat cgagccgatc agctgcacgg ggccggcgaa
      181 ggcggccacg gcgaccgcga cgacggcgcc gagcgtcgcg ccgatcgtcg ggatgaggtc
      241 cgcgatggcg acccagaacg cgagcgcagc cggatacggg acgccgagca acgccagcgc
      301 gacgaagctc acgacgccgg cgatgaggct cacgatgatg ttgccgatga catagccgcc
      361 gacccgctgg gtcgacgccc cgaggatctg ctcgaagtcc tcgcggtgct cgcgacggag
      421 cagccgcgtg acgcctcgcc gcagatccgg cagccggacg gcgaagatgg cggtcaggat
      481 cgcgacggtc agcgtgttga agaccagcgc cgcgaagctc cggacgaggc cgagcgcggt
      541 tcccgggacg tcctcaccga gcgagcgcag cttcccccga aggtcgaagc gctcgtcgag
      601 ctgtgcgatg aacccgttgc cctcctgagc ccgctcgagg tactcgggcg cctcctcgat
      661 caggttcccg atctcgcgga cgatcaccgg cacgatcagc gcgaggaacg tcgccgcgac
      721 gacgagcgtc gtcgtgtaga tgatcgcgac cgcccatccg cggcgcatac cgcgccgcgt
      781 gagcacgtcg atggcgggct ggagcccgag cgcgagcacg agcgagatca gcagcaggac
      841 tacgacgttc tggatcgcgg cgagcgcgcg gaacagcagg aggacgacga gcacgccggc
      901 cgcgacgcgc atccagtagg tgaccggagg ccaccgcgcc tcgccacccg cgggagaggc
      961 gtcgtccgag ccgcgttcgt cgccggtggc tgtcacgggt cggcctcccc gcctcgggac
     1021 cgcgcggatg gtaccccggg cgaggatcgc aggccctgga ggtcagggct cgaggacgac
     1081 cttgatggcg ccgtcgcgct tccgctggag catctcgtac gcctccggcg cggcgtcgag
     1141 cggcacgcgg tgcgtcgtga ggtccgcggt gccgagcggg tcctcgtcgc cgagcagcgg
     1201 caacaggtcg tccacccagc ggcgcacgtt cgcctggccc atccgcaccg tgatctgccg
     1261 gtcgaacagc gcgcccagcg ggaacgcgtg catccacccg acgtagacac cgacgacgga
     1321 gagcgtcccg ccgcggcgga cggccgacat cgactggtgc agcgcgtgca gccggtcggg
     1381 ctggaccttc agcgtctgga gaacgtgatc gatcgtcgcc ccgtcggcct ccatcccgac
     1441 cgcgtcgatc accgagtcgg cgccccggcc ctcggtcagg tcctcgaccg ccgcgggccc
     1501 gtccgggacg tgctcgagat cgatcacctc ggcgccgtgg cgcgccgcca gctccagtcg
     1561 ctcgggcagc cggtcgacgc cgatcacgcg gccggcgccg cggcggcgcg cgatccgcac
     1621 gcacatctga ccgatcggcc cgagtcccca gaccgcg
//
LOCUS       DASZOB010000236         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_75598, whole genome shotgun sequence.
ACCESSION   DASZOB010000236 DASZOB010000000
VERSION     DASZOB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75598"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VG709_03910"
     CDS             complement(<1..360)
                     /locus_tag="VG709_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32251.1"
                     /translation="MTTPTPASDRREPRIGLWGTFDVENLGDVLFPRIARAELARRLP
                     EATIRAYAPYGREHPIAFDGEDPAEPLGAYRPDRLAELAAELDVVVIGAGEIVHGRDE
                     LLAPIYGVAEAEVVARAP"
     gene            466..1749
                     /locus_tag="VG709_03915"
     CDS             466..1749
                     /locus_tag="VG709_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32252.1"
                     /translation="MGSHDERFDPLFQKMVEAFRGDPAAWRGVNQALRDAIVAYARER
                     SAYYREHIPPGTTFEEIPVLTRPTVRERRDELVVDGTEARQVAVRGSGSTGEPLSFFR
                     DTTQGPAEHTSGERFLRWLQGIPFDATKVWISTLPRGILGRRAAPFGLRRSDAAVHGV
                     LASTLSPRRIEREVRRWTRFRSYFVYGVASLIDVVAEHVERRGLRPARSPIAVVTTAD
                     TLTRSSEARIARAFGCPVHSWYGSNEFNGFVAGTVPGTRRYAFNPLLVHAEVLADDGS
                     PARPGSPGRIMLTDLNNYVMPFIRYDTGDVAVPSEDGFVGGFRLVDDIHGRSSETLRL
                     PSGRAVSGSALGARLFVDSDLTREVRFWQCAQVAPDVLELRVVWAREASDEAIGRIVE
                     ALRQVLDPTTVVRVRGVDRLERLPSGKAWLVRREF"
     gene            1764..>2896
                     /locus_tag="VG709_03920"
     CDS             1764..>2896
                     /locus_tag="VG709_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32253.1"
                     /translation="MGLAGETFSLLRRRDFATLMGAQWFSQAADGLVVGALAKFITFG
                     GQEGFDVEAAKSPEEAFRIVLFTFLPMLVLSPFLGVFIDRWDRRKLLIGANGVRAVAL
                     AAAVVLGGAEFRNLGSVPLYAVLLLTIASTRLLLALKGAALPATLGERDLIQGNSVSQ
                     AGSALFQLVGGGAALVASGFLAAPFVVAAGVVVYGIAVAFSFATRTLGYARPTVPLAQ
                     ELGRVLRDIAEGLREVSRRPRAGLALTSFLAVRSLATLTVLAVGFASLAFVADAGIVG
                     KAIPGVAGAAGAVAGLVVAVALKERTPPGTIVSLALFLGGIGVAAFGGVISLLGIAVS
                     AFSVGLSFFLGKIGVDTLMQQGLADAFRGRGFSFQDAVYNLSW"
BASE COUNT          351 a         1033 c         1039 g          473 t
ORIGIN      
        1 cggcgctcgg gcgaccacct ccgcctccgc gacgccatag atcggcgcca ggagctcgtc
       61 gcgcccgtgg acgatctcgc cggcgccgat gacgacgaca tcgagctccg cggccagctc
      121 cgcgaggcgg tcggggcgat acgcaccgag cggctctgcg ggatcctcgc cgtcgaacgc
      181 gatcggatgt tcccgaccat acggcgcgta ggcgcggatc gtcgcttcgg ggagccggcg
      241 cgcgagctcg gcacgcgcga tccgcgggaa cagcacgtcc ccgaggttct cgacgtcgaa
      301 ggtgccccag agcccgatgc ggggctcgcg gcgatcgctc gcgggtgtcg gggttgtcat
      361 gggttccggc tcgtccggcg tcggacgccg gcccggcaga cgtcgccaat catacggccc
      421 ccttggccgt cgcccgagga ggcggtagcc tccgcgcccg gcgtcatggg gagccacgac
      481 gagcgcttcg atcccctctt ccagaagatg gtggaggcgt tccgcggcga cccggcggcg
      541 tggcgcggcg tcaaccaagc cctccgggac gcgatcgtcg cgtacgcgcg agagcgctcc
      601 gcctactacc gcgagcacat cccgccggga acgacattcg aggagatccc ggtcctcact
      661 cgcccgaccg tgcgcgaacg ccgggacgag ctggtcgtcg acggcacgga ggcgcgtcag
      721 gtcgcggtcc gagggtccgg aagcaccggt gagccgctct cgttcttcag ggacacgacc
      781 cagggaccgg ccgagcacac ctccggcgag cggttcctgc gatggttgca ggggatcccg
      841 ttcgatgcga ccaaggtctg gatctcaacg cttcctcgag gcatcctggg ccggagggcc
      901 gcccccttcg ggctccggcg gtccgacgcc gccgtgcacg gcgtgctcgc gagcacgctc
      961 tcgccccgcc ggatcgagcg ggaggtccgt cggtggacac ggttccggtc gtacttcgtc
     1021 tacggcgtcg cgagcttgat cgacgtcgtc gcggagcacg tagagcggcg cgggctccgg
     1081 ccggcgcggt cacccatcgc ggtcgtcacg accgccgaca cgctcacgcg ctcctctgag
     1141 gcccggatcg ctcgtgcgtt cggatgtccc gtccacagct ggtacggctc gaacgagttc
     1201 aacgggttcg tcgcggggac ggtgccgggc acgcgccggt acgcgttcaa tcccctcctc
     1261 gtccatgccg aggtgcttgc cgacgacggc tcgccggccc gccccggctc ccccggccgc
     1321 atcatgctca ccgacctcaa caactacgtc atgccgttca tccgctacga caccggagac
     1381 gtcgccgtcc ccagcgagga tgggttcgtc gggggcttcc gcctcgtcga cgacattcac
     1441 ggccggagca gcgagacgct ccgcctcccg agcggcaggg ccgtgagcgg ctccgctctc
     1501 ggcgcccgac tgttcgtcga cagcgacctc acgcgcgagg tgcggttctg gcagtgcgcg
     1561 caggtcgcgc cggacgtcct cgagctccgc gtcgtgtggg cgcgggaagc gtccgacgag
     1621 gcgatcgggc gcatcgtgga ggccctacgt caggtgctcg acccgacgac cgtggtccgg
     1681 gtgcgcggcg tcgatcgcct ggaacgcctc ccgtcgggga aggcgtggct ggtccgcagg
     1741 gaattctgat acggtccgcc gccgtgggcc tcgccgggga gaccttctcg ctgcttcggc
     1801 gccgcgactt cgcgacgctc atgggagcgc agtggttctc ccaggccgcg gacgggctcg
     1861 tcgtcggcgc gctcgcgaag ttcatcacgt tcggcgggca ggaaggcttc gacgtcgagg
     1921 ccgcgaagtc gccagaggaa gccttcagga tcgtgctctt cacgttcctc ccgatgctcg
     1981 tgctgagccc gttcctcggc gtgttcatcg accgctggga ccggcggaag ctcctgatcg
     2041 gagccaacgg cgtccgggcc gtcgcgctgg cggccgccgt ggtgctcggc ggtgcggagt
     2101 tccggaacct cgggtccgtg ccgctctacg cggtcctgtt gctcacaatc gcctcgaccc
     2161 gtctcctgct cgcgttgaag ggcgcggccc tcccggcgac gctcggcgag cgcgacctga
     2221 tccagggcaa ctccgtctcg caagccggga gcgcgctgtt ccagctcgtc ggcggcgggg
     2281 ccgcgctcgt ggcctccggg ttcctggcgg cgccgttcgt cgtcgctgcc ggcgtcgtgg
     2341 tctacggcat cgccgtcgcc ttctcgtttg cgaccaggac gttgggctac gcgagaccga
     2401 cggttccgct ggcgcaggag ctcgggcggg tgctgcgcga catcgccgaa gggctccgcg
     2461 aggtctcgcg tcgcccgcgc gccgggctcg cgctgacgtc gttcctcgcg gtgcgatcgc
     2521 tcgcgacgct cacggtcctc gccgtcggct tcgcgtcgct cgcgttcgtc gcggacgcgg
     2581 ggatcgtcgg caaggcgatc ccgggcgtcg caggcgccgc gggagccgtc gcggggctcg
     2641 tcgtggcggt cgcgctcaag gagcggacgc cgccggggac gatcgtctcg ctcgctctct
     2701 tcctcggagg gatcggtgtc gcggccttcg ggggcgtgat ctcgctgctc ggcatcgccg
     2761 tcagcgcgtt ctccgtcggg ctgtcgttct tcctcgggaa gatcggggtg gacacgctga
     2821 tgcagcaggg gctcgccgac gcgttccgtg gtcgagggtt cagcttccag gacgccgtct
     2881 acaacctgtc ctggat
//
LOCUS       DASZOB010000237         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_197185, whole genome shotgun sequence.
ACCESSION   DASZOB010000237 DASZOB010000000
VERSION     DASZOB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197185"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VG709_03925"
     CDS             <1..442
                     /locus_tag="VG709_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32254.1"
                     /translation="LDPDRGRRRPQLGSAEQAVATWFEAAAWAGARGGGGRVLVQTRD
                     PGDPAIQALVRWDPSFFHRAERRRREEAGFPPGFPVFRVRGSRELPGALEALGPVTLL
                     RSSAGGQTVCLVVLRPDALAAFGARIRELASDGVVERVEAEPHL"
     gene            455..979
                     /gene="def"
                     /locus_tag="VG709_03930"
     CDS             455..979
                     /gene="def"
                     /locus_tag="VG709_03930"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWC32255.1"
                     /translation="MAILPIRTFGDPVLKEKARPVEAFDEALQRLAADMIETMQEAPG
                     VGLAAPQVGRSIRLLVYDVGDGGHALINPTLYNEWGEQIGEEGCLSLPGLYYPLRRFA
                     HVVAEGYDARGNAVTTPAEDLLARVLQHEVDHLDGVLFVDRLEGKERRQALAQLRDQA
                     LGLADERHDPARAL"
     gene            979..>1590
                     /locus_tag="VG709_03935"
     CDS             979..>1590
                     /locus_tag="VG709_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWC32256.1"
                     /translation="MRVVFLGNAPASVASLERLERSTNYIALVVTRAPRPAGRGGRPR
                     ATPVAEAARRLGLPLAEVETVKDGPGFEAIRDARPDVLVVVAYGEIIRRAVLDLPRIA
                     PVNVHFSLLPRLRGADPVRRAIRDGLDTTGVTTMRMDEGLDTGPVLLQREAPSLPDDD
                     AGALGERRSRLGAELLVDTLDALERGEITERPQDDTLATRAPKL"
BASE COUNT          203 a          573 c          585 g          229 t
ORIGIN      
        1 cctcgatccc gaccgcgggc gtcggcggcc gcagctcggc tcggcagagc aggccgtcgc
       61 gacgtggttc gaagcggcgg catgggccgg ggcccgcggt ggaggcggac gcgtgctcgt
      121 ccagacgcgc gacccagggg atcccgcgat ccaggcgctc gtccgttggg atccgtcgtt
      181 cttccatcgc gcggagcggc gccgcaggga agaggccggg ttcccgcccg ggttcccggt
      241 tttccgtgtg cgcggctcgc gggagctccc gggcgcgctc gaggcgctcg gaccggtcac
      301 gctccttcgg tcgagcgccg gaggtcagac ggtatgcttg gtcgtgctgc ggcccgacgc
      361 gctcgcggcg ttcggagccc ggatccgcga gctcgcctcg gacggtgtcg tggaacgcgt
      421 cgaggccgag ccacacctct gagagaggag cgagttggcg atccttccga tcaggacgtt
      481 cggcgacccg gtgctgaagg agaaggcacg ccccgtcgag gccttcgacg aggcgctcca
      541 gcggctggcc gccgacatga tcgagacgat gcaggaggct cccggcgtcg ggctcgccgc
      601 gccgcaggtc ggccgctcga tccgtctcct cgtgtacgac gtcggcgacg gcggacacgc
      661 gctgatcaac ccgacgctct acaacgagtg gggtgagcag atcggcgaag aggggtgcct
      721 gtcgcttccc gggctctact accctctgcg tcgcttcgcg cacgtcgtcg cggaggggta
      781 cgacgctcgc gggaacgccg tcacgacccc ggccgaggac cttctcgcgc gcgtcctgca
      841 gcacgaggtc gaccacctcg acggcgtgct gttcgtcgac cgcctcgagg gcaaggagcg
      901 ccgacaggcg ctcgcccagc tccgggatca ggcgctcggg ctcgccgacg agcggcatga
      961 tccggcgcgc gcgctctagt gcgcgtcgtc ttcctcggga acgcccccgc ctcggtcgcg
     1021 tcgctcgaac ggctcgagcg gtcgacgaac tacatagctc tcgtcgtgac gcgggctccg
     1081 cgtccggccg gtcgaggtgg ccggccccgg gcgacgccgg ttgccgaggc ggcgcggcgc
     1141 ctcgggctgc cgctcgcgga ggtcgagacc gtcaaggacg gtccgggatt tgaggcgatc
     1201 cgcgacgcgc gcccggacgt gctcgtggtg gttgcctacg gcgagatcat ccggcgtgcc
     1261 gttctcgatc tccctcggat cgcgccggtg aacgtccact tctcgctgct cccgcgcctc
     1321 cgcggcgccg accccgtccg gcgagcgatc cgcgacgggc tcgatacgac cggcgtcacg
     1381 acgatgcgga tggacgaggg cctcgacacc ggccccgttc tgcttcagcg ggaggcgccg
     1441 agccttccgg acgacgacgc cggggcactc ggcgagcgtc gctcccggct cggcgccgag
     1501 ctgctcgtcg acacgctcga cgcgctcgag cgcggggaga tcaccgaacg cccgcaggac
     1561 gacacgctgg ccacccgcgc gccgaagctc
//
LOCUS       DASZOB010000238         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_166598, whole genome shotgun sequence.
ACCESSION   DASZOB010000238 DASZOB010000000
VERSION     DASZOB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166598"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="VG709_03940"
     CDS             <1..1998
                     /locus_tag="VG709_03940"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWC32257.1"
                     /translation="LTDGEGHIDVATTRVETMLGDTGVAVHPDDGRYRALVGRRVRHP
                     FTDEELPVVSDDAVDPAFGTGAVKVTPAHDPTDFEIAERTALPRRNILTADATISGDA
                     PAEFRGLDRYEARLRVLEALRERGLVIAEERPYRHAVAHCYRCNAEIEPWLSGKQWFV
                     AVDRLKGPAKDAAVDGRIRFFPDRWRENYTTWLDNLRDWNISRQLWWGHRIPVWYCPN
                     GHEFASVDDPDACRECGEANIEQDGDVLDTWFSSQLWPFSTLGWPDETADLAYFYPTT
                     VLVTGYEILYLWVARMIMSGLYLAADIPFGHVLIHGLVRDERNRKMSKSLGNVIDPLE
                     FIGKYGADALRFALARIASPDHQNYPLGERDVEAARNFANKVWNAGRFVLRAYEGGAP
                     TLPEESARTPVERWILSRHEACRAEVDAALEAYRFSDAALAIHRFLWSELADWGLEME
                     KPRLYEGAEGERRAAASLLGWVLERTLRLLHPIMPFLTEELWQRFGTGDSIMVAKWPG
                     AHPEHRDEALDDGFSFLTSFVSEARSNSPAIQMGVAYRRTMSERQRPLVERHADLIER
                     LTGGILAIREDGELAAEVTRREGLRTTGEDAEAYRRRREKQLDELRSKLGREEAKLSN
                     PAFTERASAHAIGKARAKREEMAREVDQIERELAEIEKQLG"
     gene            1998..3281
                     /locus_tag="VG709_03945"
     CDS             1998..3281
                     /locus_tag="VG709_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWC32258.1"
                     /translation="MRFDEAVADLDRRGQHRIVPGLERIAAITELLDHPQRTYPSIHV
                     AGTNGKTTTTRLAGRILCAHGLAAGVYTSPHLSSVTERIALCDAEISEPEFAEAYAHL
                     KPFLDDTDRAGGQPVTYFEALTALAFLWFADKPVDAAVLEVGMGGRWDATNVVEAPVA
                     VVCPVSLDHPELGPTVADVAGEKAAIIKPEATAIVRRQTEDAMEVIEARIADGGADAK
                     IEDRDFGVASRDLAVGGQRLAIQGLEAEYPDLLLPLHGEHQAENAAAAVAAGESLLGR
                     ALDEGTLRDALADAAAPGRLETVARHPLVVLDGAHNVDAAGALAAAIGEALVWRRLHL
                     VVGILETKDAEGIVAALAPRADAAYATRNSNPRSLDGRVVADHCERAGVSASVHDGVA
                     AALEAAHAEADADDLILVTGSLYTVADARPRYVTP"
BASE COUNT          508 a         1155 c         1202 g          460 t
ORIGIN      
        1 ctcacggacg gcgagggtca catcgacgta gcgacgacgc gcgtcgagac gatgctgggc
       61 gacacgggcg tcgcggtcca tcccgacgat gggcggtacc gcgcgctcgt cggaaggcgc
      121 gtgcgccacc cgttcacgga cgaggagctc ccggtcgtct cggacgacgc cgtcgatccc
      181 gcgttcggta ccggcgccgt gaaggtgacg ccggcgcacg acccgacgga ctttgagatc
      241 gccgagcgaa ccgctcttcc tcggcggaac atcctgaccg ccgacgcgac gatctccggc
      301 gacgcgccgg cggagttccg cggcctcgac cggtacgagg cgcggctgcg cgtgctcgag
      361 gcgctgcgtg agcgggggct cgtgatcgcc gaggagcggc cgtaccggca cgccgtcgcg
      421 cactgctacc ggtgcaacgc ggagatcgag ccctggctct ccggcaagca gtggttcgtg
      481 gcggtggacc gcctcaaggg ccctgcgaag gacgcggccg tcgatggccg catccgcttc
      541 ttcccggacc ggtggcgcga gaactacacg acatggctcg acaacctccg cgactggaac
      601 atctcgcggc agctctggtg gggccaccgc atcccggtgt ggtactgccc gaacgggcac
      661 gagttcgcgt cggtggacga ccccgacgcg tgccgcgagt gcggcgaggc gaacatcgag
      721 caggatggcg acgtcctcga cacatggttc tcgtcgcagc tgtggccgtt ctcgacgctc
      781 ggctggcccg atgagacggc ggacctcgcg tacttctacc cgacgacggt gctcgtgacc
      841 gggtacgaga tcctctacct gtgggtcgcc cgcatgatca tgtcgggtct ctacctcgcc
      901 gccgacatcc cgttcggcca cgtcctgatc cacgggctcg tgcgcgacga gcgcaaccgc
      961 aagatgtcga agtcgctggg caacgtgatc gacccgctgg agttcatcgg gaagtacggc
     1021 gcagacgcgc tgcgcttcgc gctcgcgagg atcgccagcc ccgatcacca gaactacccg
     1081 ctcggggagc gcgacgtcga ggccgcgcgc aacttcgcga acaaggtgtg gaacgccggc
     1141 cggttcgtcc tccgcgcgta cgagggcggc gcgccgacgc ttcccgagga aagcgcccgc
     1201 actcccgtgg aacggtggat cctgtcgcgg cacgaggcgt gccgggcgga ggtcgacgcc
     1261 gcgctcgagg cgtatcgctt ctccgacgcc gcgctcgcga tccatcgctt cctctggtcg
     1321 gagctcgccg actgggggct cgagatggag aagccgcgcc tgtacgaggg tgccgaaggt
     1381 gagcgccgcg cggccgcgtc gctcctcggc tgggtgctcg agcggacgct ccggctgctg
     1441 cacccgatca tgccgttcct caccgaggag ctctggcagc ggttcggcac cggcgactcg
     1501 atcatggtcg cgaagtggcc gggggcgcat cccgagcacc gcgacgaggc gctcgacgac
     1561 gggttctcgt tcctcacgtc gttcgtgagc gaggcccgga gcaacagccc tgcgatccag
     1621 atgggagtcg cctaccgacg gacgatgtcg gaacggcagc gaccgctcgt cgagcggcat
     1681 gccgacctta tcgaacgcct caccggcggg atcctcgcga ttcgcgagga cggcgagctc
     1741 gcggccgagg tgactcgccg tgagggcctg cggacgaccg gcgaagacgc ggaggcgtac
     1801 cgacggcggc gcgagaagca gctcgacgag ctccggtcga agctcggccg cgaggaggcg
     1861 aagctctcga accccgcgtt cacggaacgc gccagtgccc acgccatcgg caaggcgcgc
     1921 gccaagcgcg aggagatggc gcgagaggta gaccagatcg aacgcgagct ggcggagatc
     1981 gagaagcagc tcggctgatg cggttcgacg aggccgtcgc cgacctcgac cgccgcgggc
     2041 agcaccggat cgttccggga ctcgagcgga tcgcagcgat cacagagctg ctcgaccatc
     2101 cgcaacgcac gtatccctcg atccacgtcg cggggaccaa cggcaagacg acgacgacgc
     2161 ggctcgcggg ccggatcctg tgcgcgcacg ggcttgcggc cggtgtgtac acgtcgccgc
     2221 acctctcgtc ggtgacggag cggatcgcgc tgtgcgacgc cgagatctcg gagcccgagt
     2281 tcgccgaggc gtacgcgcac ctgaagccct tcctcgacga tacggaccgg gcaggcggcc
     2341 agccggtgac gtacttcgag gcgctgacgg cgctggcgtt cctctggttc gccgacaagc
     2401 ccgtcgatgc cgcggtcctg gaggtcggga tgggcgggcg ctgggacgcg acgaacgtcg
     2461 tcgaggcgcc ggtcgcggtc gtgtgtcccg tctccctcga tcacccggag ctcggcccca
     2521 cggtcgccga cgtcgcgggc gagaaggccg cgatcatcaa gccggaggcg acggcgatcg
     2581 tgcggcgaca gaccgaagac gcgatggagg tgatcgaggc gcggatcgcc gatggcggcg
     2641 ccgacgcgaa gatcgaggac cgcgacttcg gggtcgcctc gcgggacctc gccgtcggcg
     2701 gccagcgcct ggcgatccag ggactcgagg ccgagtaccc ggacctgctc cttccgcttc
     2761 acggcgagca ccaggcggag aacgccgccg ccgccgtcgc ggccggcgag tcgctgctcg
     2821 ggcgggcgct cgacgagggg acgctgcgcg acgcgctcgc ggacgccgcg gcgccggggc
     2881 gcctcgagac ggtcgcccgc catccgctcg tcgttctcga cggcgcgcac aacgtcgacg
     2941 ccgctggggc gctcgcggcg gcgatcggcg aggcgctcgt ctggcgccgg ctgcatctcg
     3001 tggtcggcat cctggagacg aaggacgccg aagggatcgt ggccgcgctc gcgccgcggg
     3061 ccgatgcagc gtacgcgacg cgcaactcga acccccgctc ccttgatgga cgcgtcgtcg
     3121 cggatcactg tgaacgagcc ggggtctccg catcggtcca cgatggggtc gccgcggctc
     3181 tcgaagcggc gcacgccgag gccgacgccg acgatctgat cctcgtgacg ggctctctct
     3241 atactgtcgc ggacgcgcgc ccgcgctacg tcaccccatg agcgaagaga ccctcgtcat
     3301 cgtgaagccc gacgccgtcc ggcgt
//
LOCUS       DASZOB010000239         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_115371, whole genome shotgun sequence.
ACCESSION   DASZOB010000239 DASZOB010000000
VERSION     DASZOB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115371"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VG709_03950"
     CDS             complement(<1..364)
                     /locus_tag="VG709_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWC32259.1"
                     /translation="MERGLISEDAIQSKIRELGDRITEDYRDREPLLVGVLKGAFVVM
                     ADLSRHIRLPLEFDFMAVSSYGASTKTSGVVRILKDLDHDIRGRHVLLVEDIVDSGLT
                     LAYLLRNLRARKPGSLEVC"
     gene            complement(438..1481)
                     /gene="tilS"
                     /locus_tag="VG709_03955"
     CDS             complement(438..1481)
                     /gene="tilS"
                     /locus_tag="VG709_03955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWC32260.1"
                     /translation="MPQNPPAVARVVERVTATVRRHGMFDPGETVVVACSGGPDSVCL
                     LHTLHRLRRLFRIRLAVFHFDHRLRDGSADDAAYVERQAERLKVPLLRREAIDAPTPG
                     QSIEAWARLARYAALTEEARGIDAPRAAVGHTLDDQAETVLLGITRGGGIDAVAGMPP
                     VGTAPPRGFPIVRPLLETTRDEVRAFCRALRLRPREDPSNLDRRFLRNRLRHEVLPAL
                     EASLDRNVKVTLARIAENVRSDAEYLDAVASREAKHVTTVGEREVLLDAARLAALPEP
                     VGVRVVQQALRVAAAAAGGSDVDAGAAHLTGVLDLAAGRPGRRVDLPGGLLALRTREY
                     VRVSSASDGRGRR"
     gene            complement(1510..2562)
                     /locus_tag="VG709_03960"
     CDS             complement(1510..2562)
                     /locus_tag="VG709_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWC32261.1"
                     /translation="MAQGTAERLLDWGAAARIGSGIAGSGSLSPRDRRAMDAELGEVL
                     READALVGDLTGLRLSGPPSRPWVMARGEWVTQNLRAFREILEPITERLMRDRLDGPT
                     APLRRGALAFQLGGILGYLGRKVLGQYDLFQPADDRDLLYFVGDNLVELERRFRFAPR
                     DFRLWLSLHEVTHRFQFEGVPWMRGYLFGLIEEYLSTLELDPRKLVDALRRAYEEARS
                     SGRWRELGIVSLLMTPEQRDTFRRMQALMSLLEGHGNFVMDRLGADHVRGAERMRRVL
                     HERRHRAGWNRLVQRAVGVDVKVRQYDLGERFVAGVVERAGLDGFSRVWEAPENLPTL
                     DEVPRPDRWVERVAAP"
     gene            complement(2568..>3048)
                     /locus_tag="VG709_03965"
     CDS             complement(2568..>3048)
                     /locus_tag="VG709_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32262.1"
                     /translation="QPSEPQQREEQEPAGATAQEQPEENAPAAGAPGAAEGRTEAGPE
                     DRDAGEEEAARVTEERKQPEGVQAAPVTGESPPEAEEVRGEDPEKKVEAAAPQAKATD
                     EAAPAEKPQTGATEVDLDQETFDRVKQEQLDKGVAQAVAEARARAAAIKAAREKAAG"
BASE COUNT          405 a         1105 c         1111 g          427 t
ORIGIN      
        1 cgcacacctc gaggctgccg ggcttgcgcg cgcggaggtt ccggagaagg tacgcgagcg
       61 tcaggccgga gtcgacgatg tcttcgacga gaagaacgtg gcggcctcgg atgtcgtggt
      121 cgaggtcctt gaggatccgc acgacccccg acgtcttggt cgaggcgccg tacgaagaga
      181 ccgccatgaa gtcgaactcg agcggcagcc ggatgtgccg cgacaggtcc gccatcacga
      241 cgaaggctcc cttgaggacg ccgacgagca gcggctcgcg gtcgcggtag tcctcggtga
      301 tgcggtcgcc gagctcgcgg atcttcgact ggatcgcgtc ctcggagatg aggccgcgct
      361 cgatgtcctt ctcgaactcg aaccgcggcg tgcgaggatc gtctccgcct tgtgatcggt
      421 cgagccgggt ctccacgcta ccttcgtccc cgtccgtcgc tcgcggaaga gacgcgaaca
      481 tactctctgg tccgcaaggc cagcaacccg ccgggaagat cgactcggcg tcccgggcgt
      541 cccgccgcga ggtcgagcac gccggtgagg tgtgcggcac cggcgtcgac gtcggatccg
      601 ccggccgcgg cggccgcgac gcgtagcgcc tgctgcacga cccgcacgcc gaccggctcg
      661 ggaagggcgg ccagccgcgc cgcgtcgagg agcacctcgc gctcgccgac cgtcgtcaca
      721 tgtttggcct cgcgtgacgc gaccgcatcg aggtactcgg cgtcggagcg gacgttctcg
      781 gcgatcctcg cgagcgtaac cttgacgttc cggtcgagcg aggcctcgag cgccggtagg
      841 acctcgtgcc ggagccggtt gcgcaggaac cgccgatcga ggttcgacgg atcctctcgc
      901 gggcggaggc gaagcgcgcg gcagaacgcg cggacctcgt cgcgcgtcgt ctcgaggagc
      961 ggacggacga tcgggaaccc ccgcggcggg gccgtcccga cgggcggcat gccggcgacg
     1021 gcgtcgatgc cgccgcccct cgtgatcccg aggaggacgg tctcggcctg atcgtcgagc
     1081 gtgtggccga cggccgcgcg cggcgcgtcg atcccccggg cctcctcggt gagggcggcg
     1141 tagcgcgcga gccgcgccca ggcctcgatg gactggcccg gagtcggcgc gtcgatcgcc
     1201 tcgcggcgca gcaacgggac cttcagccgt tcggcctggc gctcgacgta cgcggcgtcg
     1261 tccgcggagc cgtcgcgcag gcgatggtcg aagtgaaaga cggcgagccg gatccggaac
     1321 agccgccgga gccggtgaag ggtgtggagc aggcagaccg aatccgggcc acccgagcac
     1381 gcgacgacga cggtctcccc cgggtcgaac atgccgtgac gccggaccgt cgcggtgacg
     1441 cgctcgacga cgcgggcgac ggccggcggg ttctgcggca tcaggacccg aacgctgcga
     1501 cgaacgccgt cacggagcgg cgacgcgctc gacccagcgg tcggggcgcg ggacctcgtc
     1561 gagcgtcggg aggttctccg gcgcctccca cacgcgcgag aacccgtcga gccccgcacg
     1621 ttcgacgacg cccgcgacga accgctcgcc gaggtcgtac tggcggacct tcacgtcgac
     1681 gccgacggcg cgctggacga gccggttcca cccggcgcgg tggcgccgtt cgtgcagcac
     1741 gcggcgcatc cgctcggcac cgcggacgtg gtcggcgccg agccggtcca tcacgaagtt
     1801 gccgtggccc tcgagcagcg acatgagcgc ctgcatcctc cggaaggtgt cgcgctgctc
     1861 cggcgtcatg aggagcgaca cgatcccgag ctctcgccat cgtccgctgc tacgtgcctc
     1921 ctcgtacgca cggcggagcg cgtcgacgag cttgcgggga tcgagctcga gcgtcgacag
     1981 gtactcctcg atgagcccga acaggtaccc gcgcatccac gggaccccct cgaactggaa
     2041 ccggtgcgtg acctcgtgca gcgagagcca cagccggaag tcgcgcggag cgaagcggaa
     2101 ccggcgctcg agctccacga ggttgtcgcc gacgaagtag aggagatcgc ggtcgtcggc
     2161 cggctggaag aggtcgtact ggccgaggac cttgcggccg agatatccga ggatcccgcc
     2221 gagctggaac gcgagggcgc cgcggcgcag cggcgcggtc gggccgtcga ggcgatcgcg
     2281 catcaggcgc tcggtgatcg gctcgaggat ctcccggaac gcgcggaggt tctgcgtgac
     2341 ccactctccc cgcgccatga cccacggacg cgacggaggc cctgagagcc ggagaccggt
     2401 caggtcgccg acgagggcgt ccgcttctcg gaggacctcg ccgagctccg cgtccatcgc
     2461 gcggcggtcg cgcggggaca gcgatccgga gcccgcgatg ccgcttccga tgcgggcggc
     2521 ggccccccag tcgaggaggc gctccgcggt gccctgcgcc acgcgcgcta gcccgcggcc
     2581 ttctcgcgcg cggccttgat cgcggcagct cgcgcgcggg cctcggccac ggcctgggcg
     2641 acgcccttgt cgagctgctc ctgcttcacg cggtcgaacg tctcctggtc gagatcgacc
     2701 tcggtcgccc cggtctgcgg cttctcggcc ggggccgcct cgtcggtcgc cttcgcctgc
     2761 ggcgccgcgg cctcgacctt cttctcgggg tcctcgccgc ggacttcctc ggcctccgga
     2821 ggcgactcgc cggtgaccgg agcggcctgg acgccctcgg gctgcttccg ctcctcggtc
     2881 acgcgggcgg cctcctcctc gcccgcgtcg cggtcctcag gccccgcttc cgtgcgtccc
     2941 tccgcggcgc ccggtgcgcc ggcagcgggt gcgttttcct ccggctgctc ctgggccgtc
     3001 gcacctgccg gctcctgctc ctcccgttgc tgcggctccg agggctgc
//
LOCUS       DASZOB010000240         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_5467,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000240 DASZOB010000000
VERSION     DASZOB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5467"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VG709_03970"
     CDS             complement(<1..200)
                     /locus_tag="VG709_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWC32263.1"
                     /translation="MTAIHLDTDLGSDTDDLCALAMLLGWPGVELVGVTTCIDPDGSR
                     VGFVRPALEVGGRPNVPVARGAG"
     gene            complement(197..1210)
                     /locus_tag="VG709_03975"
     CDS             complement(197..1210)
                     /locus_tag="VG709_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWC32264.1"
                     /translation="MERSPSGSRVTISDVAESARVSKTTVSHVLSGKRPVAPLTRNRV
                     LAAIAELGYRPDGVARTLRTRRSQMVAMIVPDIGNPFYTALARGLDHGMGGSEYRVVI
                     CNTDGRRDQELAFVAEMCDRGVDGIVLDSFAVSERALREVTENAVPVAWIGGGTLDHP
                     GVDTVKADDERGAFDATIHLARAGRRRIAMITGTPGSGTPRNDGYARALEAAGMSVDT
                     SLVRVGEWTSAGGARAMREIFEAREPPDAVFCANDLMAIGALETVRARGLRVPEDVAL
                     VGFDDIDAAAMVTPPLTTVLNPAYETGLTAGQLLASRMTGEYRGEARHAVLPCALVVR
                     ASA"
     gene            1527..>1913
                     /locus_tag="VG709_03980"
     CDS             1527..>1913
                     /locus_tag="VG709_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWC32265.1"
                     /translation="MRNRRWRWTAALGAVTLVATACIGGGGQTQQQAQPVERVEPPTE
                     ETTITFSSWIGESPEIKQLVKDFEEEYPTITVELQNVPAERSREKLLTQVAGGNPPDS
                     AYVDLSVVEDFGTRGALVNLDNYIAQS"
BASE COUNT          300 a          687 c          662 g          264 t
ORIGIN      
        1 ccggcgccgc gcgcgactgg cacgttcggg cgtcctccga cctcgagcgc ggggcgcacg
       61 aacccgacac ggctgccatc aggatcgatg caagtggtga ccccgacgag ctcgacgccc
      121 ggccatccga gaagcatggc cagcgcgcag aggtcgtcgg tgtccgagcc caggtcggtg
      181 tcgaggtgga tcgccgtcac gccgaagcgc gtacgacgag cgcgcacggc aggaccgcgt
      241 gccgggcctc gccgcggtat tcgcccgtca tgcgcgaagc gaggagttgc ccggcagtga
      301 gccccgtctc gtaggcgggg ttcagcacgg tcgtcagcgg aggcgtgacc atggccgcgg
      361 cgtcgatgtc gtcgaacccc acgagcgcga cgtcctccgg gacgcggagc ccccgtgccc
      421 ggacggtctc cagcgcgccg atcgccatca agtcgttcgc gcagaacact gcgtccggcg
      481 gctcgcgtgc ctcgaagatc tctcgcatcg cacgcgcgcc tcccgcgctc gtccactccc
      541 cgacacggac caacgacgtg tcgacgctca tccccgccgc ctcgagcgcg cgcgcgtagc
      601 cgtcgttccg cggcgtcccc gagcccggcg ttcccgtgat catcgcgatg cgtctccggc
      661 ccgcccgcgc gagatgaatc gtcgcgtcga acgcgccacg ttcgtcgtcg gccttcaccg
      721 tgtccacccc gggatgatcc agcgttcccc ctccgatcca ggccacggga acggcgttct
      781 cggtgacctc gcggagcgct cgctcgctga cggcgaacga gtcgagcacg atcccatcga
      841 cgccgcggtc gcacatctcg gcgacgaagg cgagctcctg gtcgcgcctt ccatcggtgt
      901 tgcagatgac cacgcggtac tcgctgccgc ccatcccgtg gtcgagccct cgcgcgagcg
      961 ccgtgtagaa ggggttgccg atgtcgggga cgatcatcgc caccatctgt gagcggcgcg
     1021 tgcggagagt gcgggcgacg ccgtcgggcc ggtacccgag ttccgcgatc gccgcgagca
     1081 cgcggttgcg cgtgagcggg gcgaccggac gcttccccga aaggacgtgc gagacggtcg
     1141 tcttgctcac ccgcgcactt tcggcgacgt ccgagatcgt tacacgtgaa ccggaaggcg
     1201 atcgttccac cggagcgacg agcgtgcggc gtggtcgcgg cctgtgtcaa gcgtgggcgc
     1261 gcccggaacg ggcccggacg tggcgatttg acagttccga gaaccctcgg taatgctcgg
     1321 accgcccggg cacaaatcgg tttgccggtc tgcggcaccg cccgacgcgt cgaagaagga
     1381 ggagcgagcg gccttcgagc gagacgaggc gcggcgcacc accgctccgg ctcgaccgcg
     1441 gaccgcccgc tccctctctc cgccgcgaag cgcgtcgcaa cccgcgccga cggatgccct
     1501 cgcaggcgga taggaaggga ggccaggtga ggaatcgcag gtggcgctgg acggcggcgc
     1561 tcggcgccgt gacgctggtc gcgacggcgt gcatcggcgg cggcggtcag acgcagcaac
     1621 aggcccaacc cgtcgaacgg gtcgagcccc cgaccgagga gaccacgatc acgttctcct
     1681 cgtggatcgg cgagtcgccc gagatcaagc agctcgtcaa ggacttcgaa gaggagtacc
     1741 ccaccatcac ggtcgagctc cagaacgtgc cggccgaacg gtcgcgcgag aagctgctga
     1801 cgcaggtcgc cggcgggaac ccgcccgact cggcgtacgt ggacctctcg gtcgtcgagg
     1861 acttcggcac gcgcggcgcg ctcgtgaacc tcgacaacta catcgcacag agc
//
LOCUS       DASZOB010000241         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_96034, whole genome shotgun sequence.
ACCESSION   DASZOB010000241 DASZOB010000000
VERSION     DASZOB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96034"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VG709_03985"
     CDS             <1..866
                     /locus_tag="VG709_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWC32266.1"
                     /translation="VMLTDPDGRVLEFVEKPTLADLREHFQVHDEREFDSLPLLTNAG
                     FYLLDPKVMREAARDPRLQELRRSRLDFGKDFLPWLVEAGFRVQASPVRRIGDLGNVE
                     DYVTTMVDVLQGRFESVLRLMGPAYDPGANVWIAPESLKMTDERTGKTLEEKMAEGLV
                     TIGPAVRVGKYCEIGPGVVISESNLDDDVEVDEGAYVSRSSIRDGAIIGAGATVRKSV
                     VGSMSELRSSPAQPTVIEDYVALGDEVVVQPGVRLSGGVSVYPRLKIPRGIRVPPQAE
                     LATPKDVLELL"
     gene            869..>1569
                     /locus_tag="VG709_03990"
     CDS             869..>1569
                     /locus_tag="VG709_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWC32267.1"
                     /translation="MRIGLALPHYDFSFPDGGPVSWERLADAASRAESLGFDSVWVSD
                     HFFLALTRYGGGPEPHGSVEPLAALAGLATVTERVRLGTLVLATTFRHPAIVAKAAAT
                     VDLVSGGRLELGLGAGWYEDEHRAFGYEFGSVGDRFEILEENVEVIGRLLAAEQPVTW
                     RGRRFRIEEAYNRPAPVQKPRPPVIVGGKGGRRLLRLVARHADGWNTVWQWTPEAYAE
                     RVEALWDACTAARRDP"
BASE COUNT          217 a          540 c          584 g          228 t
ORIGIN      
        1 gggtgatgtt gaccgacccc gacggacgcg tcctcgagtt cgtcgagaaa ccgacgctcg
       61 cggacctccg cgagcacttc caggtccacg acgagcggga gttcgactcg ttgccgctgc
      121 tcacgaacgc cgggttctac ctcctcgatc cgaaggtcat gcgcgaggcc gcgcgcgacc
      181 cgcgtctcca ggagctgcga cgcagccgcc tcgatttcgg caaggacttc ctcccgtggc
      241 tcgtcgaggc gggcttccgc gtgcaggcgt cccccgtgcg gaggatcggc gacctcggca
      301 acgtcgagga ttacgtcacg acgatggtcg acgtgctgca gggccggttc gagtcggtcc
      361 tccggctgat ggggccggcg tacgaccctg gagcgaacgt ctggatcgcg cccgagtcgc
      421 tcaagatgac cgacgagcgc accggcaaga cgctcgagga aaagatggcg gaggggctcg
      481 tgacgatcgg gcctgcggtg cgcgtcggca agtactgcga gatcgggccc ggcgtcgtga
      541 tcagcgagtc gaacctcgac gacgacgtcg aggtcgacga gggcgcgtac gtgtcgcggt
      601 cgtcgatccg cgacggcgcg atcatcggcg cgggcgcgac cgtccggaag tcggtcgtcg
      661 ggtccatgtc ggagctccgc tcgtcgccgg cgcagccgac ggtgatcgag gactacgtcg
      721 cactcggcga cgaggtcgtc gtgcaaccgg gcgtccggct ctccggcggc gtcagcgtct
      781 atccgcgcct caagatccct cgcggcatcc gggtcccgcc gcaggcggag ctcgcgacgc
      841 ccaaggacgt cctcgagctg ctctgagcat gcggatcggg ctcgcgctcc cccactacga
      901 cttctcgttc cccgacggcg ggccggtgtc gtgggagcgc ctcgcggatg cggcgagccg
      961 tgccgagtcg ctggggttcg actcggtgtg ggtctccgac cacttcttcc tggcgctcac
     1021 gcggtacgga ggcgggccgg agccgcacgg cagcgtcgag ccgctcgcgg cgctcgccgg
     1081 gctcgcgacc gtcaccgagc gcgtccggct cgggacgctc gtgctcgcga cgacgttccg
     1141 ccaccccgcg atcgtcgcga aggccgcggc gaccgtcgac ctcgtgtcgg gcgggcgcct
     1201 cgagctcggc ctcggcgccg gctggtacga ggacgagcac cgcgcgttcg ggtacgagtt
     1261 cggctcggtc ggcgaccgct tcgagatcct cgaggagaac gtcgaggtga tcgggcgact
     1321 gctcgcggcg gagcagccgg tcacgtggcg cgggagacga ttccgcatcg aggaggccta
     1381 caaccggccg gcgccggtcc agaagccgcg gccgccggtg atcgtcggcg ggaaaggcgg
     1441 gaggcggctg ctgcgcctcg tcgcgcggca cgccgacggc tggaacaccg tctggcagtg
     1501 gaccccggag gcgtacgccg agcgcgtaga ggcgctctgg gacgcgtgca cggccgcccg
     1561 gcgcgaccc
//
LOCUS       DASZOB010000242         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_55655, whole genome shotgun sequence.
ACCESSION   DASZOB010000242 DASZOB010000000
VERSION     DASZOB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55655"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VG709_03995"
     CDS             complement(<1..358)
                     /locus_tag="VG709_03995"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWC32268.1"
                     /translation="MGIESSIERVRALIRDIPDFPQPGIVFKDITPLLADEAGFSSVI
                     DMIVVHYGRGNIDKVVGIEARGFILASPVAYHFGAGFIPVRKVGKLPWETESEEYELE
                     YGTETLEIHRDALRPAE"
     gene            complement(368..1603)
                     /locus_tag="VG709_04000"
     CDS             complement(368..1603)
                     /locus_tag="VG709_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019059619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWC32269.1"
                     /translation="MRSSVFLVELGAVIVGLAVLARLAGRFGFSPIPLYLLAGLAFGE
                     GGVLPLVTAERFIETGAEIGVILLLLMLGLEYSGEELTETLRSAPRAGLVDLLENATP
                     GFAAGVVLGWGVLPSVLLAGITYISSSGVVAKVIADLGWIGNREVPVVLSLLVIEDLA
                     MAVFLPVVAVLLVGVGAAQAAVIVGVALVTVRVFLFVAVRFGDSLSRVIFSRSDEALL
                     LTMFGLTLVVAGVAERLQISAAVGAFLVGIAVSGPAAARAQSLLSPLRDLFGAAFFVF
                     FSLEIDPGTIPPVIVPALLLAAAGVATKAFTGWWAARGVGIGRRGRRRAGAVLVARGE
                     FSIAIAALGLSAGVRQDLGPLTAAYVLTLALLAPILARIASPPVRRGSVPIRGPGPEE
                     RPAGGTMAAERPRDVPGGE"
     gene            complement(1607..2089)
                     /locus_tag="VG709_04005"
     CDS             complement(1607..2089)
                     /locus_tag="VG709_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HWC32270.1"
                     /translation="MAEVRETKLPGLGIRYEFMTSRGNRVGVVHHRTGRRELLLYERD
                     DPDTCRDVVALDEDDSRTLAELLGGSRVEEQLDRLQEVEGLAIDWLPVSEGTPYADGT
                     IGDTRARTRTGVSVVAVLRGEEAFPAPDPNFRLIAGDTLLVVGTPRGIEQLAVLLRTG
                     "
BASE COUNT          362 a          807 c          767 g          281 t
ORIGIN      
        1 gctccgcggg gcggagcgcg tcgcggtgga tctcgagcgt ctcggtcccg tactcgagct
       61 cgtactcctc cgactcggtc tcccagggaa gcttcccgac cttgcggacc gggatgaacc
      121 cggccccgaa gtggtacgcg accggcgaag cgaggatgaa gccgcgagct tcgatcccga
      181 cgaccttgtc gatgttcccg cggccgtagt gcacgacgat catgtcgatc acggacgaga
      241 accccgcctc gtcggcgagc agcggcgtga tgtccttgaa gacgatcccg ggctggggga
      301 agtcggggat gtcccggatc agcgctcgaa cgcgctcgat gctcgactcg atccccatcc
      361 gcgctcctta ctcgccgccc gggacgtctc gggggcgctc ggcggccatt gtgccaccgg
      421 cgggccgctc ctcggggccg gggccgcgga tcggaacgga tccgcggcgt accggcgggg
      481 aggcgatccg cgcgaggatc ggggcgagga gcgcgagcgt gagcacatac gccgcggtga
      541 gcggaccgag gtcctggcgg acgccggcgc tgagcccgag tgccgcgatc gcgatcgaga
      601 actcgccccg cgcgacgagc acggcaccgg cacgccttcg gcccctgcga ccgatcccca
      661 cgccgcgggc cgcccaccat cccgtgaacg ccttcgtcgc gacgcccgcg gcggcgagca
      721 gcagcgcggg gacgatcacg ggcgggatcg tcccggggtc gatctcgagg ctgaagaaga
      781 cgaagaatgc ggccccgaac aggtcgcgga gggggctcaa gagcgactgc gcacgcgccg
      841 ctgctggacc ggagaccgcg atgcccacga ggaacgcccc gacggcggcc gagatctgga
      901 gccgctcggc gacccccgct accacgagcg tcaggccgaa catcgttagc agcagcgcct
      961 cgtcggaccg gctgaagatc acgcggctga gcgagtcccc gaaccgcacg gcgacgaaca
     1021 ggaagacgcg caccgtcacg agcgcgaccc cgacgatgac ggccgcctgc gcggcgccca
     1081 ctccgacgag gagcacagcg accaccggaa ggaagacggc catcgcgagg tcctcgatca
     1141 cgagtagcga tagcacgacc ggcacctcgc ggttcccgat ccatccgagg tccgcgatca
     1201 ccttcgcgac gacccccgac gaggagatgt aagtgatgcc ggcgaggagg acgctgggga
     1261 gcacgcccca gccgagcacg accccggccg cgaaccccgg cgtcgcgttc tcgaggagat
     1321 cgacaaggcc ggcgcgaggc gccgaacgga gcgtctccgt cagctcttct cccgagtact
     1381 cgagcccgag catgagcagc agaaggatca cgccgatctc cgccccggtc tctatgaatc
     1441 gctcggcggt cacgagcggg agcactccgc cttcgccgaa cgcgagccct gcgaggaggt
     1501 acagcgggat cggcgagaac ccgaacctcc cggcgagccg tgccagcacc gcgagcccca
     1561 cgatgaccgc cccgagctcg accaggaaga ccgagctccg cacggcctac ccggttcgga
     1621 gcaggaccgc gagctgctcg atgccgcggg gcgtgccgac tacgagcaga gtgtcgcccg
     1681 cgatgaggcg gaagttcgga tcgggcgccg ggaacgcctc ctctccacgg aggacggcca
     1741 ccacggacac gccggtgcgg gtcctcgccc gcgtgtcccc gatcgtccca tccgcgtacg
     1801 gcgtcccctc gctcaccggg agccagtcga tcgcgagccc ctcgacctcc tggagacggt
     1861 cgagctgctc ctcgacccgc gagccgccga gcagctctgc gagcgtcctt gagtcgtcct
     1921 cgtccagcgc cacgacgtcg cggcacgtgt cggggtcgtc gcgctcgtag agcaagagct
     1981 cgcggcggcc cgtccggtgg tgcacgacgc cgactcggtt cccacggctc gtcatgaact
     2041 cgtagcggat cccgagcccc ggaagcttcg tctcccgaac ttcggccatc atcttctccg
     2101 cttggacttc ggcttcttct tcggcttccg ccgcgcgggc ggcgctccag ggggacgagg
     2161 tgaggccgcg acgggctcga gcggacgctc gccctccgcg ggggcctcgg gctcgac
//
LOCUS       DASZOB010000243         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_75726, whole genome shotgun sequence.
ACCESSION   DASZOB010000243 DASZOB010000000
VERSION     DASZOB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75726"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..753)
                     /locus_tag="VG709_04010"
     CDS             complement(61..753)
                     /locus_tag="VG709_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWC32271.1"
                     /translation="MSAASGGLVHRKRAASGDPEGALVLLHGRGADEQDLVPLLDALD
                     PGRRLVGLTPRGPLALPPGGAHWYVVREVGFPDPATFYPSFHALAAWLGALPTELGVP
                     WERTVLGGFSQGAVMAYALALGQGRPPPAGVVALSGFVPTVEGFELGLEDRHGFPVAI
                     GHGSFDPVIGVEFGRAARERLAAAGLSVTYRESPMPHAIDPGFVRELQGWLPAVTGAR
                     APSGTDSEASGT"
     gene            complement(750..1814)
                     /locus_tag="VG709_04015"
     CDS             complement(750..1814)
                     /locus_tag="VG709_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWC32272.1"
                     /translation="MGKTRAMPRLRRVDCSAPGIVRRRRGRGFEYYDERGERIDDPDV
                     LERIRALVIPPAWTDVWISPYPFGHIQAVGTDAAGRRQYLYHVRWRARRDQEKCDEMI
                     RFARALPELRAVAAEHLRLEGMPRERALACAVRLLDRGFFRIGTEDYAEQNQTYGLAT
                     MQKRHVTLRPGYVITFDYAAKGGKRRIQSIVDPDVYGVVDVLKRRRSGPELLAYKQRH
                     RWLDVTSADINEYVKEVTGADFSAKDFRTWNGTVLAAVALAVSARAATTKTARKRAIA
                     RAIKEVAHYMGNTPAVARASYIDPRVFDRFRAGVTIGGALERVGDVELGQPSTQGTIE
                     EAVLDLLEEDDASPAIEKVA"
     gene            complement(1820..>2090)
                     /locus_tag="VG709_04020"
     CDS             complement(1820..>2090)
                     /locus_tag="VG709_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWC32273.1"
                     /translation="EPDSEGATQAAADVEAEAREELEFVAARVRALPAELRVPLVLRD
                     VEGWSNDDVARMLGLSVAATKSRVRRARMRLRAEREAWSGRRFGS"
BASE COUNT          288 a          766 c          755 g          281 t
ORIGIN      
        1 ggcaggcgcg atccgcgtgg cagcacagca tcccgccgac caaggcgctg cgctactcga
       61 tcacgttccg gacgcttcgg aatccgtccc gctgggcgcc cgcgctcccg tgacggcggg
      121 aagccacccc tgcagctcgc ggacgaaccc cgggtcgatc gcgtgcggca tgggcgactc
      181 gcggtaggtg accgagagcc cggcggcggc cagccgctcg cgcgccgcgc ggccgaactc
      241 gacgccgatg accgggtcga agctgccgtg gccgatcgcg acgggaaagc cgtgccggtc
      301 ctcgaggccg agctcgaagc cctcgaccgt cgggacgaag ccgctgagcg cgacgacgcc
      361 ggcgggcggt ggacgaccct ggccgagagc gagcgcgtac gccatgacgg cgccctgcga
      421 gaaaccgccc agcaccgtcc gctcccacgg gacgccgagc tccgtgggga gcgccccgag
      481 ccaggcagct agcgcgtgga agctcgggta gaacgtcgcc ggatcgggaa agccgacctc
      541 gcgaacgacg taccagtggg cgccgccggg cggaagcgcg agcggcccgc gcggcgtgag
      601 gccgacgagc cggcgcccgg gatcgagcgc atcgaggagc ggtacgaggt cctgctcgtc
      661 cgccccgcgc ccgtgcagga ggacgagcgc cccttccggg tcgccgctcg ccgcgcgctt
      721 gcggtggacg agtccgccgg acgcggcgct cacgccacct tctcgatcgc cggcgacgca
      781 tcgtcctctt cgagcagatc gagaaccgcc tcttcgatcg tgccctgagt ggagggctgg
      841 cccagctcga cgtcgcccac ccgctccagc gccccgccga tcgtcacgcc ggcgcggaag
      901 cgatcgaaca cgcgcgggtc gatgtacgac gcgcgggcca cggcgggcgt attgcccatg
      961 tagtgggcga cctccttgat cgcgcgagcg atcgcccgct tgcgcgccgt cttggtcgtc
     1021 gcggcgcgcg ccgagacggc gagcgcgacc gcggcgagga cggtcccgtt ccacgtgcgg
     1081 aagtccttcg ccgagaagtc ggcccccgtc acctccttga cgtactcgtt gatatccgcg
     1141 gacgtgacat ccagccagcg gtgccgctgc ttgtacgcga ggagctccgg tcccgaccgg
     1201 cgacgcttga ggacgtcgac gacgccgtag acgtctgggt cgacgatcga ctggatgcgg
     1261 cgcttgccgc ccttcgcggc gtagtcgaag gtgatgacgt atcccgggcg cagcgtgacg
     1321 tggcgcttct gcatggtggc gagcccgtac gtctggttct gctcggcgta gtcctcggtc
     1381 ccgatgcgga aaaagccgcg gtcgagcagg cgcaccgcgc acgcgagcgc gcgttcccgc
     1441 ggcatcccct cgagccgcaa gtgctcggcg gccacggcgc gcagttccgg gagcgcgcgc
     1501 gcgaagcgga tcatctcgtc gcacttctcc tggtcgcgcc gcgcccgcca gcgaacgtgg
     1561 tagaggtact gcctgcgtcc ggcggcgtcc gtcccgacgg cctggatgtg tccgaacggg
     1621 tacggggaga tccagacgtc ggtccaggcg ggcgggatga cgagcgcgcg gatgcgctcg
     1681 agcacgtcgg ggtcgtcgat gcgctcgccg cgctcgtcgt agtactcgaa cccgcggccg
     1741 cgtctgcggc ggacgattcc cggggcggag cagtcgacgc gtcggaggcg cggcatcgcc
     1801 cgcgtctttc ccacgcaggc taggagccga accgccgtcc gctccacgct tcccgctcgg
     1861 cgcggagccg catccgcgcg cggcggacgc ggctcttcgt cgctgcgacc gacaacccga
     1921 gcatccgcgc gacgtcgtcg ttcgaccatc cttcgacgtc gcgcaggacg agcgggacgc
     1981 gcaactccgc cggcagcgcg cgcacccgcg cggcgacgaa ctcgagctcc tcgcgggcct
     2041 ctgcttcgac gtccgcggcc gcctgcgtag cgccttcgct gtcgggctcg
//
LOCUS       DASZOB010000244         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_55680, whole genome shotgun sequence.
ACCESSION   DASZOB010000244 DASZOB010000000
VERSION     DASZOB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55680"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1520)
                     /locus_tag="VG709_04025"
     CDS             complement(<1..>1520)
                     /locus_tag="VG709_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWC32274.1"
                     /translation="LVANVAEGRIEQGASTITQQLARNAIAGVGEDDSLARKLREAHT
                     AVRLEEEYSKDEILELYLNDVYFGGGAHGIGTAAQRYFGVSPKKLRLPQAALLAGAIA
                     APGRFSPYVDEDAARARRNVVLDRMAALGWIGDKRAEKAKSAPLGYREGEPFALRQPR
                     APFFVEYIKRQILRDERFGDTRRARRRLLYQGGLRIDTTLDLSLQRAARRTARRHLPR
                     PNDPEAAVVAVDPRTGAIVTLLGGKDFDRQKYNLATQGRRQAGSAFKPFTLVAALERG
                     ISSMRRYDGSSPKRIRLPNGKVWKVRNAGGSSYGRIDLRRATQSSVNTVFAQLIMDVG
                     PERVARAARRMGIESRLPAVPSLALGTASVAPLDMASAFGTLARGGVRCEPFAIAKVT
                     DRSGEVLFEDSRGNCERVVRNPIAARVVSMLRRVVEDGTGTEARLPGPPVFGKTGTTD
                     DFADAWFVGCARQLCTSTWVGHVEGRIPMEDVHGTTVYGGTFPARIWHDFMQTAMD"
BASE COUNT          220 a          542 c          518 g          240 t
ORIGIN      
        1 gtccatggcg gtctgcatga agtcgtgcca gatccgtgcc gggaaggttc cgccgtagac
       61 ggtggtcccg tggacgtcct ccatcgggat gcgtccctcg acgtgtccga cccacgtcga
      121 cgtgcacagc tgtcgagcgc agccgacgaa ccatgcgtcg gcgaagtcgt cggtcgtccc
      181 cgtcttcccg aagaccgggg gcccagggag acgcgcctcg gtccccgttc cgtcttcgac
      241 gacgcggcga agcatcgaca cgacgcgggc cgcgatcggg ttccgcacca cgcgctcgca
      301 gttgccacgc gagtcttcga acagcacctc gcccgaacgg tcggtcacct tggcgatcgc
      361 gaacggctcg catcgcaccc ctccgcgggc gagcgttccg aacgccgacg ccatgtcgag
      421 cggcgcgacg ctggcggtcc cgagcgccag cgatgggacg gccgggaggc ggctctcgat
      481 gcccatgcgt cgggccgcgc gcgcgacgcg ctcgggcccg acgtccatga tgagctgcgc
      541 gaacaccgta ttgaccgacg actgagtcgc gcggcggagg tcgatccgtc cgtagctcga
      601 gccgccggcg ttccgcacct tccagacctt tccgttcggc aaccggatgc gcttcggcga
      661 ggagccgtcg tacctcctca tcgaggagat gccgcgctcg agtgccgcga cgagcgtgaa
      721 cggcttgaac gcagatccgg cctgccggcg tccctgcgtc gccaggttgt acttctgccg
      781 gtcgaagtcc ttgcctccga gaagcgtgac gatcgcgccg gtccgggggt cgacggcgac
      841 cacggctgcc tcggggtcgt tcggtcgcgg gaggtgacgg cgagcggtac gacgcgccgc
      901 ccgctgcagc gagaggtcga gcgtcgtgtc gatcctgaga ccgccctggt acaacagccg
      961 gcgtcgcgcg cgcctcgtgt ccccgaaccg ctcatcgcgg aggatctggc gcttgatgta
     1021 ctcgacaaag aacggagccc gcggctgtcg gagcgcgaac ggttcgccct cgcggtaacc
     1081 gagaggcgcg ctcttggcct tctcggctcg cttgtcgccg atccagccca gcgccgccat
     1141 gcgatcgagc acgacgttgc gacgggcgcg cgccgcgtcc tcatcgacgt acggcgagaa
     1201 ccggccgggg gccgcgatgg cgcctgccag cagcgccgcc tgaggcagcc gcagcttctt
     1261 cgggctcacc ccgaagtagc gctgggcggc cgtgccgatc ccgtgggccc cgccgccgaa
     1321 atagacgtcg ttcaggtaca gctccaggat ctcgtccttc gaatactcct cctcgagacg
     1381 aaccgccgta tgggcctcgc ggagcttgcg ggccagcgaa tcgtcctcgc cgacaccggc
     1441 gatagcgttc cgcgcgagct gctgcgtgat cgtcgacgcc ccttgctcga tccgtccttc
     1501 ggcgacgttc gcgacgaggg
//
LOCUS       DASZOB010000245         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_85952, whole genome shotgun sequence.
ACCESSION   DASZOB010000245 DASZOB010000000
VERSION     DASZOB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85952"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VG709_04030"
     CDS             <1..1205
                     /locus_tag="VG709_04030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWC32275.1"
                     /translation="LLRGARKAWDEALDLGELSGYRNAQASVLAPTGTIRFMMDCATT
                     GVEPDFSLVKSKKLVGGGEITIVNKTVAMALEKLGYAPAEVDQVVAYVDDRNTVVGAP
                     FMKTEHYPVFDCAIGERAIHYMGHVKMMGAVQPFISGAISKTVNMPEQATVEDVRELL
                     VDAWKLGVKAIAIYRDNCKVAQPLSKKADGAAAAPALPPVPLDKPARRRLPDDRTEIG
                     RKFRVGDYEGYIHVGLFDDGTPGDVFVDIAKEGTTLAGLMNSFMISVSLGLQYGVPLE
                     VYVSKFSHMRFEPSGQTNDADIRVAKSLVDYIFRWLGKKFLDTDTQEELGIMSPEVRR
                     RLAQAQDVLEGRAEAPEQAAPASPPGQAALFNAWEDAVECAKCGGRMVRTGSCYTCRD
                     CGHNTGCS"
     gene            1503..>2014
                     /locus_tag="VG709_04035"
     CDS             1503..>2014
                     /locus_tag="VG709_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWC32276.1"
                     /translation="MPAGDVLWNPLTGEKALVLESAEETGGERIVAEFAVERGGFVHG
                     GDHVHDHVKEHFDVEEGEIAFNLDGEERVVGPGEQLTVEPGTWHRWWNAGQGEVRIRA
                     SVEPALRFQEMIAVFWGLCADGHTDSRGTPSPLYGALLAMRYRDEIRFRRPPQAVQRL
                     LFPPLAAPASG"
BASE COUNT          334 a          682 c          707 g          291 t
ORIGIN      
        1 acctcctgcg cggcgcgcgc aaagcgtggg acgaggcgct cgacctcggc gagctgagcg
       61 gctaccgcaa cgcccaggcg tccgtgctcg ctcccaccgg gacgatcagg ttcatgatgg
      121 actgcgccac gacgggggtc gagcccgact tctcgctcgt caagtcgaag aagctcgtgg
      181 gcggcggcga gatcacgatc gtgaacaaga cggtcgcgat ggcgctcgag aagctcggct
      241 acgcgccggc cgaggtcgac caggtcgtcg cctacgtcga cgaccgaaac acggtcgtcg
      301 gcgcgccgtt catgaagacg gagcactacc ccgtcttcga ctgcgcgatc ggcgagcgcg
      361 cgatccacta catgggccac gtgaagatga tgggtgccgt ccagccgttc atcagcgggg
      421 cgatctcgaa gacggtgaac atgcccgagc aggccacggt cgaggacgtc cgcgagctcc
      481 tcgtcgacgc ctggaagctc ggcgtcaagg cgatcgcgat ctaccgcgac aactgcaagg
      541 tcgcgcagcc gctctcgaag aaggccgacg gcgccgccgc ggcgcccgcg ctaccgccgg
      601 tgccgctcga caagccggcc cgccggcgcc tcccggacga ccggacggag atcggccgca
      661 agttccgcgt cggcgactac gagggctaca tccacgtcgg cctcttcgac gacgggacgc
      721 ccggcgacgt cttcgtcgac atcgccaagg aggggacgac cctcgccggc ctcatgaact
      781 cgttcatgat ctcggtctcg ctcgggctcc agtacggcgt gccgctcgag gtctacgtct
      841 cgaagttcag ccacatgcgg ttcgagccgt cgggccagac gaacgacgcg gacatccgcg
      901 tcgccaagtc gctcgtcgac tacatcttcc gctggctcgg caagaagttc ctggacacgg
      961 acacgcagga ggagctcggc atcatgagcc cggaggtccg ccgccggctt gcccaggcgc
     1021 aggacgtcct cgagggcagg gccgaggccc cggagcaggc ggccccggcg tcgcccccgg
     1081 gccaggcagc tctcttcaac gcctgggagg acgcagtcga gtgcgccaag tgcggcggcc
     1141 ggatggttag aacgggcagc tgctacacct gccgagactg cggccacaac accgggtgta
     1201 gctaactaac tgtcctgcct acggacgttg gaaggcggct gatcggcggc cgtctccgag
     1261 cgagcggggg gtccgtcgcc cgttgtagtg gtcgagcgag tgtggcaacg accggttccg
     1321 ctgtcgattg agcctatgca acgcggtacg ccgccggcga gcagcaagtc ccccgcctcg
     1381 tctggagcgg gcacgggaag tagcgatcac cggccgctga cactgcgctt gcgcgagggc
     1441 gcttccgtgc gcgtcgagcg gggcgcttga acgtgcgccg gtgcgcggct tacgatgcgg
     1501 ccgtgcccgc cggtgacgtg ctctggaacc cgctcaccgg cgagaaggcg ctcgtgctcg
     1561 agtccgcgga agaaacgggc ggcgagcgaa tcgtcgccga attcgcggtc gagcggggcg
     1621 ggttcgtaca cggcggcgac catgttcacg accacgtgaa ggagcacttc gacgtcgagg
     1681 aaggcgagat cgcgttcaac ctcgacggcg aggaacgcgt cgtcggcccc ggcgagcagc
     1741 ttacagtcga accgggcacg tggcaccgct ggtggaacgc gggacagggg gaagtgcgaa
     1801 tccgggcgtc cgtggagccc gcgctgagat tccaggagat gatcgcggtg ttctgggggc
     1861 tctgcgccga cggccacacc gactctcgcg gaacgccctc gccgctctat ggcgccctgc
     1921 tcgcgatgcg ctatcgcgac gagatccgtt tccgacggcc gccccaggcc gtgcagcggc
     1981 ttctcttccc accgctcgcc gcgccggcga gcgg
//
LOCUS       DASZOB010000246         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_115500, whole genome shotgun sequence.
ACCESSION   DASZOB010000246 DASZOB010000000
VERSION     DASZOB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115500"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VG709_04040"
     CDS             complement(<1..698)
                     /locus_tag="VG709_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC32277.1"
                     /translation="MRVLVWHGWLLDGTGSNVYTAKVAEVWRRSGHDVVLVCQERHVD
                     RFPFVDAVGVVADGVVSEVESTGAPPEEGSVVLLRPDIGALLPVFVFDEYEGFEPKRF
                     VELSDEELNAYLERNVRALAAAAERFPPDVVVAGHVVPGPVIARRAIGAGTYVAKVHG
                     SDLEYAVRLQPRYRELAREGLEGARAISGASHDVLRRAVELVPGVADRIRVIPPGVDI
                     ERWQPRPRREALLEL"
     gene            766..2073
                     /locus_tag="VG709_04045"
     CDS             766..2073
                     /locus_tag="VG709_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC32278.1"
                     /translation="MAAKIRMADPEQPVSATLPSRLEDIRRSEQTMIAVRWAGVVFAL
                     VQVLAYQELPYPRGVKAAAFALIAVLAVANVLITVATRRVLSLGGALKLSVAAFALDI
                     LVTSGFVWLYAFDPLTAMWAVLFIVPLEGAIRFQLAGALLGWGIATVSYLLRELFRIE
                     VYPSSAVGPRGFGFQPESISFRMGIALIVALVAGLMSRNLMRQRGHLAEALDELSRID
                     TLRARLVTTLAHDVRGPLTTIRGVFRTLRRYGERISANDRDELIESGDRQAGRLERLA
                     SDLLDLARLERGRLDLQLENVRLDDAVERAMYFADAERRFEIRVAPDLVVRADPARLE
                     QIIVNLATNALRHGAPPFVVEGRPGTDGHVELAFRDHGVGVPDAERDQLFEPFSTDAE
                     SGSVGLGLAIVRALTEAQGGRVAYEENDPRGACFRVELLRGAT"
BASE COUNT          285 a          788 c          727 g          324 t
ORIGIN      
        1 agctcgagga gcgcctcgcg acgcgggcgg ggctgccagc gctcgatgtc gacaccgggc
       61 gggatcacgc ggatgcggtc ggcgacgccc gggacgagct ccacggctct gcgcaggacg
      121 tcgtggctcg ctccggagat cgctcgcgcg ccctccaggc cttcgcgcgc gagctcccgg
      181 tacctcggct ggagccgtac cgcgtactcg agatcgctcc cgtggacctt cgcgacgtag
      241 gttcctgccc cgatcgcgcg gcgcgcgatg accggccccg gcacgacgtg gccggcgacg
      301 acgacgtccg gcgggaagcg ctcggccgcc gccgcgagcg cgcgcacgtt gcgctcgagg
      361 tacgcgttca gctcctcgtc gctgagttcg acgaagcgct tcggctcgaa gccctcgtat
      421 tcgtcgaaga cgaacaccgg gagcagcgcg ccgatgtcgg ggcgaagcag gacgacgctt
      481 ccctcctccg gcggcgcgcc cgtcgactcc acctctgaga cgactccgtc ggccacgacg
      541 ccgaccgcgt cgacgaaggg gaaccggtcg acatgacgct cctggcacac gaggaccacg
      601 tcgtgaccgc tccgccgcca tacctcggcc accttggccg tgtagacgtt cgagccggtc
      661 ccgtcgagga gccagccgtg ccatacgagg acgcgcacgc gcgggagtgt aggcggacca
      721 ccgcttttcg cacttcccta tcgccgtgga ccttcgtgcc ctaccatggc cgcgaagatc
      781 cgcatggcag accccgaaca gcccgtctcc gcgacgctgc ccagccgcct cgaggacatc
      841 cgccggtccg agcagacgat gatcgcagtc cgctgggccg gcgtcgtctt cgcgctggtc
      901 caggtcctcg cgtaccagga gctcccgtac cctcgagggg tcaaggccgc ggcgttcgcg
      961 ctcatcgcgg tgctcgccgt cgccaacgtc ctcatcacgg tcgcgacgag gcgcgtcctc
     1021 tcgctcgggg gagcgctcaa gctttccgtg gcggcgttcg cgctcgacat cctcgtgacc
     1081 tcgggcttcg tgtggctcta cgcgttcgac ccgctcacgg cgatgtgggc cgtgctgttc
     1141 atcgtccccc tcgagggcgc catccggttc cagctcgccg gcgcgctgct cgggtggggg
     1201 atcgcgacgg tctcatacct cctgcgagag ctgttccgca tcgaggtcta cccgtcctcg
     1261 gccgtagggc cgcgaggctt cggcttccaa ccggagagca tctcgttccg catggggatc
     1321 gctctgatcg tcgcgttggt cgcggggctc atgtcccgca acctcatgcg tcagcggggc
     1381 cacctcgccg aggcgctcga cgagctgtcc cgcatcgaca cgctccgcgc gcgcctcgtc
     1441 acgacgctcg cgcacgacgt ccgcggtccg ctcacgacga tccgcggagt gttccgcacg
     1501 ctccggcggt acggggagcg gatctccgcg aacgatcgcg acgagctcat cgaatcgggc
     1561 gaccgccagg ccggccggct cgagcggctc gcgtccgacc tgctcgacct cgcgcggctc
     1621 gagcgcggaa ggctcgacct gcagctggag aacgtgcggc tcgacgacgc cgtcgagcgc
     1681 gcgatgtact tcgccgacgc ggagcgtcgg ttcgagatcc gcgtggcccc ggacctggtc
     1741 gtccgcgccg acccggcacg ccttgagcag atcatcgtga acctcgcgac caacgcgctc
     1801 cgccacggcg ccccgccgtt cgtcgtggag ggacggcccg gcaccgacgg gcacgtcgag
     1861 ctcgcgttcc gcgatcacgg cgtcggcgtt cccgacgcgg agagggatca gctcttcgaa
     1921 ccgttctcga ccgacgcgga gagcggctcc gtggggctcg ggctcgcgat cgtccgcgcg
     1981 ctgaccgagg cgcagggcgg gcgcgtcgcc tacgaggaga acgacccgcg cggcgcttgc
     2041 ttcagggtcg agctgctgcg cggcgccacg taggcgtcga ggaacgtcgc gagctcgtcc
     2101 gccgtccgct cgggcgcctc gaac
//
LOCUS       DASZOB010000247         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_66197, whole genome shotgun sequence.
ACCESSION   DASZOB010000247 DASZOB010000000
VERSION     DASZOB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66197"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..>1764)
                     /gene="pnp"
                     /locus_tag="VG709_04050"
     CDS             complement(72..>1764)
                     /gene="pnp"
                     /locus_tag="VG709_04050"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWC32279.1"
                     /translation="LMVEAGASEISEAEILDALDIAHEEIKKLCAAQRELAEKAGKPK
                     AEVEVPQVDESLFATLTEQFGERLRDATQVVDKLERQEATKAVEGEALEALAGSPDDP
                     QDGERRAAAQLAFDKLEKETIRRRIAIEKKRPDGRAADEIRDIWIEVGVAPRTHGSAV
                     FTRGQTQAFSVASLGTTRDEMRLDTLGLETSKRYFHHYNFPPFSVGEAGFMRGPKRRD
                     IGHGALAERALVAVVPDPEEFPYTIRVVSDILESNGSSSMASVCGSSLARMDAGVQIT
                     RPVAGIAMGLIKEGDEYIVLSDIAGVEDHLGDMDFKVAGTSKGITALQMDIKITGVTF
                     EILRDALSQAKQGRTHILDKMAEAIQGPRPELSRHAPRISQIQIDTEKIGMVIGKGGE
                     TIRALSEEFDAQIDIDDDGIVRVFAGSGEQGDALVDRIRSMTKEVEVGDEFTGKVVKT
                     TTFGAFVELSKGNDGLLHISNVKPGERVDAVEDVLSRGDEIAVRVVEVDRERGRVGLR
                     LAEDPDIAGKSAAELAGVGTGDKGGGGRGGGDRRPRRDGDRGGRGRDGGGRRERR"
BASE COUNT          222 a          662 c          579 g          301 t
ORIGIN      
        1 gcgcacggcg ggcaccgcct ccgtgacgac cctgactccc gcgtcgaggg tggttgtctg
       61 cggacggtcg ctcagcgccg ctcccgccgc ccgccgccgt cgcgcccgcg tccgccacgg
      121 tcgccgtcac ggcgcggacg acggtcgcca ccgccgcgcc cgccgccgcc cttgtcgccg
      181 gtgccgacgc ccgcgagctc cgcggccgac ttgccggcga tgtccgggtc ctccgctagg
      241 cggagaccga cgcggccgcg ctcgcggtcg acctcgacca cccgcacggc gatctcgtcg
      301 ccgcggctca ggacgtcctc gaccgcgtcg acgcgctcgc cgggcttcac attcgagatg
      361 tgcagcaggc cgtcgttgcc cttcgacagc tcgacgaacg caccgaaggt ggtggtcttc
      421 accaccttgc cggtgaactc gtcgccgacc tcgacctcct tggtcatcga gcggatgcga
      481 tccacgagcg catcgccctg ctcgccgctg cccgcgaaga cgcgaacgat gccatcgtcg
      541 tcgatgtcga tctgcgcgtc gaactcctcg gagagcgcgc ggatcgtctc cccgcccttg
      601 ccgatgacca tgccgatctt ctcggtgtcg atctggatct ggcttatccg cggagcgtgc
      661 ctcgagagct cggggcgcgg cccctggatc gcctcggcca tcttgtcgag gatgtgcgtg
      721 cgtccctgct tcgcctgcga gagcgcgtcg cgcaagatct cgaacgtcac gcccgtgatc
      781 ttgatgtcca tctgcagcgc ggtgatgccc ttggaggtgc cggcgacctt gaagtccatg
      841 tcgcctaggt ggtcctccac gcccgcgatg tcggacagca cgatgtactc gtcgccctcc
      901 ttgatgaggc ccatcgcgat gccggcgacc ggccgcgtga tctgcacgcc cgcgtccatc
      961 cgcgccagcg agctgccgca gaccgacgcc atcgacgacg agccgttcga ctcgaggatg
     1021 tccgacacga cgcgaatcgt gtacgggaac tcctcgggat ccggcacgac cgccaccagc
     1081 gcccgctcgg cgagcgcgcc gtggccgatg tcgcggcgct tcggaccgcg catgaagccg
     1141 gcctccccga ccgagaacgg cgggaagttg tagtggtgga agtagcgctt cgaggtctcg
     1201 aggccgagcg tgtccagccg catctcgtca cgcgtcgtgc cgaggctcgc gacggagaac
     1261 gcctgggtct ggccacgggt gaacacggct gagccgtgcg tgcggggggc cacgccgacc
     1321 tcgatccaga tgtcgcggat ctcgtccgcg gcacggccgt cgggccgctt cttctcgatc
     1381 gctatccgcc ggcggatcgt ctccttctcg agcttgtcga aggccagctg tgcggctgcc
     1441 cgccgctcgc cgtcctgcgg atcgtcgggg gagcccgcga gcgcctcgag cgcctccccc
     1501 tcgaccgcct tggtcgcctc ctggcgctcg agcttgtcga cgacctgggt ggcatcgcgc
     1561 agcctctcgc cgaactgctc ggtgagcgtc gcgaacaggc tctcgtcgac ctgcgggacc
     1621 tccacctcgg ccttcggctt gccggccttc tcggccagct cgcgctgggc ggcgcagagc
     1681 ttcttgatct cctcgtgcgc gatgtcgagc gcgtcgagga tctccgcctc ggagatctcg
     1741 ctcgcgccgg cctcgaccat cagg
//
LOCUS       DASZOB010000248         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_126210, whole genome shotgun sequence.
ACCESSION   DASZOB010000248 DASZOB010000000
VERSION     DASZOB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126210"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="aceA"
                     /locus_tag="VG709_04055"
     CDS             complement(<1..1112)
                     /gene="aceA"
                     /locus_tag="VG709_04055"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HWC32280.1"
                     /translation="MAPTTSMAHEAERLDRDWRTAERWRASERLYDAEDVVRLRGSVR
                     VEHTLARLGAERLWELLTGERHVATLGALTGGQAVQMVKAGLPAIYLSGWQVAADANL
                     GEQVYPDQSLYPANSAPALVRRLNNALRRADQIEWAEGRNARRWLVPIVADAEAGFGG
                     ALNAYELMKAFVEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSQFVRTLSAARLAADV
                     LDVPTILVARTDALSATLLTSDVDERDHDFVTGERTPEGYFVVSSGLDAALARSLAYA
                     PYADVLWFETSTPDLGEAREFARAVHERFPGKLLAYNCSPSFNWKRHLGDDEIASFQE
                     TLAELGYRFQFITLAGFHSLNAGMFELAKGYAADAMT"
     gene            1259..2191
                     /locus_tag="VG709_04060"
     CDS             1259..2191
                     /locus_tag="VG709_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWC32281.1"
                     /translation="MEAFLEVARVRSVSRAADALFVTQPAITARIKGLERALDATLFV
                     RTSRGMKLTESGEAFLPYAKRALETLADGRRAVNTVERGGGGRLSVGAAPAVSTYVLP
                     SVLKRFAESHPRISVSVRTGHSEEIVELVLREQVDVGLVRALRHPEIATTPLYEDRLV
                     LVVEPSHRFASSGSIDLEQMSREQLVLFDRTSSYHEVTSAFFRRAGVVPAAVMELDNI
                     EAAKKMVEEGFGVALLPHTAVESADAEPVGRQIVAIRRKDRSAELGAVASFLTTAEQT
                     APEIAREPPRPGGASPRAELRGRQSRARAHARAR"
     gene            2295..>2611
                     /locus_tag="VG709_04065"
     CDS             2295..>2611
                     /locus_tag="VG709_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32282.1"
                     /translation="MEELEGGSGGARHVRGVWRIEERSADLACLDEVVCSAHGGDDLP
                     RDADASGELLVRKRRDGVDREAAKPRLVETHVAAGQIQLLEVTTDGFGREALLAQIDD
                     RGRA"
BASE COUNT          395 a          902 c          929 g          385 t
ORIGIN      
        1 gtcatcgcgt cggccgcata tcccttggcc agctcgaaca tccccgcgtt gagcgagtgg
       61 aagccagcca gcgtgatgaa ctggaagcgg tacccgagct ccgcgagcgt ctcctggaag
      121 ctcgcgatct cgtcgtcgcc gagatgccgc ttccagttga aggatggcga gcagttgtag
      181 gcgaggagct tgccggggaa gcgctcgtgc acggcccgcg cgaactcgcg cgcctcgccg
      241 aggtcgggcg tcgacgtctc gaaccagagg acgtccgcgt acggcgcgta cgcgagcgac
      301 cgggcgaggg cagcgtcgag gccgctcgag acgacgaagt acccctcggg cgttcgctct
      361 ccggtcacga agtcgtggtc gcgctcgtcg acgtcgctcg tcaggagcgt cgcgctgagc
      421 gcgtcggtgc gggcgacgag gatggtgggg acgtcgagga cgtcggccgc gagccgtgcg
      481 gcagagagcg tgcggacgaa ctgactcgtc gggacgagca ccttgccgcc gaggtgcccg
      541 cacttcttct cggacgcgag ctggtcctcg aagtggacgc ccgccgcccc ggcctcgacg
      601 aacgccttca tgagctcgta cgcattgagc gcaccgccga agccggcctc cgcgtccgcg
      661 acgatcggaa ccagccagcg gcgcgcgttc cggccctccg cccactcgat ctgatcggcg
      721 cgccggagag cgttgttcag tcgccggacg agtgccggcg cactgttcgc cgggtagagg
      781 ctctggtccg gatacacctg ctcgccgaga ttcgcgtcgg ctgccacctg ccagccgctg
      841 aggtagatcg ccggcagacc ggccttcacc atctggacgg cttgcccgcc cgtcagcgca
      901 ccgagggtcg cgacgtggcg ctcgcccgtc agcagttccc acagccgctc ggcgccgaga
      961 cgcgcgaggg tgtgctcgac gcggacggaa ccgcggaggc ggacgacgtc ctcggcgtcg
     1021 tagaggcgct cgctcgcgcg ccagcgctcg gcggtccgcc agtcgcggtc gagccgctct
     1081 gcctcgtgag ccatcgaagt cgtgggcgcc atcgcgctct tccctccttg ctcgcgtccc
     1141 gagggcgagt cctcgccgcg ccgggcgttt ctacgcccaa gcctacgccc gtcctcgcaa
     1201 tcgatcaaat cgtttctctc gatccttgct ataaacatta tctatgctag ccgcccaggt
     1261 ggaagcgttc ctcgaggtcg cgcgcgtccg gagcgtgagc cgtgcagcgg acgcgctctt
     1321 cgtcacccag cctgcgatca cggcgcggat caagggactc gagcgggcgc tcgacgcgac
     1381 gctcttcgtg cggacgtcca gggggatgaa gctcaccgag tccggcgagg cgttcctccc
     1441 ctacgcgaag cgagcactcg agacgctggc ggacggacgc cgcgccgtca acacggtcga
     1501 gcgaggcgga ggcggacgtc tttcggtcgg agctgctccc gccgtcagca cgtacgtcct
     1561 cccttccgtc ctgaaacggt tcgcggagag ccatccgagg atctcggtga gcgtgaggac
     1621 ggggcattcg gaggagatcg tcgagctcgt gctccgtgaa caggtcgacg tcggcctcgt
     1681 acgggccctc cggcaccccg agatcgcgac gacgccgctc tacgaggatc ggctcgttct
     1741 cgtcgtcgaa ccgagccacc gcttcgcttc ctcgggaagc atcgacctcg agcagatgag
     1801 ccgcgagcag ctcgtactgt tcgaccggac gtcgagctac cacgaggtga cgagcgcatt
     1861 cttccggcgc gcaggcgtcg tcccggccgc cgtcatggag ctcgacaaca tcgaggcggc
     1921 gaagaagatg gtcgaggagg ggttcggcgt cgcgctgctc ccgcacaccg cggtcgagag
     1981 cgccgacgcc gagccggtcg gccgtcagat cgtcgcgatc cggcgcaagg acagaagcgc
     2041 cgagctcggg gcggtggcgt cgttcctcac gacggcggag cagaccgcgc ccgagatcgc
     2101 ccgagagccg cctcgacccg gcggagcgtc tcctcgcgcg gaactgcgag gacgacagtc
     2161 cagagctcgg gcccacgcgc gcgcccggta agcgcgacgc ggagcgcgcg caggtcggcg
     2221 cgcgcggctc gaagctcggc gacgattccg cgtgcgctct ctgggtcgag gcgattgacg
     2281 cgcccgcgaa ggtcgtggaa gagctcgagg gtggaagcgg cggcgctcgg cacgtccgcg
     2341 gcgtctggag gatcgaggag cgctcggccg atctcgcgtg cctcgacgag gtcgtgtgct
     2401 ccgcgcacgg cggggacgac ctcccgcggg acgccgacgc gagcggcgag ctcctcgtcc
     2461 ggaagcgccg cgatggcgtc gaccgcgagg cggcgaagcc gcgtctcgtc gagacgcacg
     2521 tcgccgcggg gcagatccag ctcctcgagg taacgacgga cggcttcggc agggaagccc
     2581 ttctcgcgca gatcgacgat cgaggccgcg c
//
LOCUS       DASZOB010000249         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_32880, whole genome shotgun sequence.
ACCESSION   DASZOB010000249 DASZOB010000000
VERSION     DASZOB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32880"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VG709_04070"
     CDS             complement(<1..1298)
                     /locus_tag="VG709_04070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32283.1"
                     /translation="MTDAATESTVRFREARPADALVVAALHLENLPAAFLPTLGLRFL
                     ERLYTAMIGDEDGLVVVAVAEDDGSVVGFVGAVRSVPRFYRRFALKHGIAALLAAGWR
                     LTRPGTLRAAAETVLYARDVEAPVAEILSAAVRPEYRGTGVGLELIEDVVARLGTTGV
                     PAVRTTINTRHRVAASAFERQGFRRVGRVAVHRGKPNDVLVRDMRLAKRDGPRRLGGP
                     EGNGELIMPRPIRVAHVATVGPTVRLLLIEQLRRLRDEGFDVAAISAPDPRGPELEAE
                     GIRYLPWPGATRAFDLASDARAFASLYRILRRERFDVVHTHNPKPGFLGRFAARLAGV
                     PCVVNTVHGLYATPDDGAPRKALVLSLEWLAGRVSHLELFQSEEDLRWARRIGLVDPP
                     PGAYLGNGVDVRRFDPQAVPGERVAELRDELGIPRDAPVVG"
     gene            complement(1295..>2031)
                     /locus_tag="VG709_04075"
     CDS             complement(1295..>2031)
                     /locus_tag="VG709_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HWC32284.1"
                     /translation="YRRMPGSRRVVPALVGQLRRMRRLKQATQVMQVSDPARRYAGWL
                     RIFTPELQEELLAADAADALRDHDPAADYPRHYAALNGSRAADHLNRLMYVDLKSWLP
                     DAYMEKTDKATMACSLEARLPLLDHRLVELAFRIPGRSKIRGLSTKRIFKRAVAGVVP
                     PAVLRKRKHGFSVPTDPWFRGELRTFALDVLLDERTKSRGYFDARVVERLWREHVSGR
                     HVWDMQLWTLLNLELWHRFFIDGEAV"
BASE COUNT          306 a          737 c          734 g          254 t
ORIGIN      
        1 ccgacgaccg gcgcgtcgcg cgggatgccg agctcgtcgc gcagctcggc gacgcgctca
       61 cccggcacgg cctgcgggtc gaagcggcgc acgtcgaccc cgttgccgag gtacgcgccc
      121 ggaggcgggt cgacgagccc gatccggcgc gcccaccgga ggtcctcctc gctctggaac
      181 agctcgaggt gggagacccg accggccagc cactccagcg agagaacgag cgccttcctc
      241 ggcgccccgt cgtcgggcgt cgcgtagaga ccgtgcacgg tgttgacgac gcacggcacc
      301 ccggcgagcc gagcggcgaa ccgtccgagg aaccccggct tcgggttgtg cgtgtggacg
      361 acgtcgaagc gctcacgacg caggatccgg tacagcgacg cgaacgcccg ggcatcggag
      421 gcgaggtcga aggcgcgcgt cgcgcccggc cacgggaggt agcggatccc ttcggcctcg
      481 agctccgggc cacgaggatc gggcgccgag atcgccgcga cgtcgaagcc ctcgtcgcgg
      541 agccggcgca gctgctcgat cagcagcagc cggacggtgg ggcccacggt cgcgacgtgc
      601 gcgacgcgga tcggcctcgg catgatgagc tcgccgttcc cttccggccc gccgagccgg
      661 cgcggaccat cccgtttcgc cagacgcatg tcgcggacca ggacatcgtt gggcttgccg
      721 cggtgcaccg cgacgcgacc gacgcgccgg aacccctggc gttcgaacgc cgaggcggcc
      781 acacggtgcc gcgtgttgat cgtggtccgg acggccggca caccggtggt tccgagccgc
      841 gccaccacgt cctcgatgag ctcgagcccg acaccggtcc cccggtactc aggtcggacg
      901 gccgccgaga ggatctcggc gacgggcgcc tcgacgtccc gcgcgtagag cacggtctcc
      961 gccgcggcgc gcaaggtgcc gggacgcgtg agccgccagc ccgcggccag cagcgccgcg
     1021 atcccgtgct tgagcgcgaa gcgacggtag aaccgcggga ccgaacgaac ggccccgacg
     1081 aaaccgacga cggacccgtc gtcctcggcg acggcgacga cgacgaggcc gtcctcgtcc
     1141 ccgatcatcg ccgtgtacag ccgttcgagg aaccgcaggc ccagcgtcgg caggaaggcg
     1201 gccggcaggt tctcgaggtg cagcgcggcg acgacgaggg cgtccgcggg ccgcgcctct
     1261 cggaagcgga cggtgctctc ggtcgccgcg tcggtcacac ggcctcgccg tcgatgaaga
     1321 accggtgcca gagctcgagg ttcagcagcg tccagagctg catgtcccag acgtgccgcc
     1381 cggagacgtg ctcgcgccac agccgttcga cgacgcgagc gtcgaagtag ccgcggctct
     1441 tcgtccgttc gtcgagcagc acgtcgagcg cgaacgtgcg cagctcgccg cggaaccacg
     1501 ggtccgtcgg gaccgagaag ccgtgcttgc gcttgcggag cacggccggc gggacgacgc
     1561 ccgcgaccgc tcgcttgaat atccgcttcg tcgacaaccc gcggatcttc gagcgcccgg
     1621 ggatgcggaa cgccagctcg acgagccggt gatcgagcag cggaagacgc gcctcgaggc
     1681 tgcacgccat cgtcgccttg tcggtcttct ccatgtacgc gtcgggcagc cacgacttga
     1741 ggtcgacgta catcagccgg ttcaggtggt ccgccgcccg agagccgttc agcgccgcgt
     1801 agtgacgcgg gtagtcggcc gcggggtcgt ggtcccgcaa cgcatcggcg gcatctgccg
     1861 cgaggagctc ctcctgaagc tccggcgtga agatccgcag ccatcccgcg taccgtcgcg
     1921 ccggatcgga gacctgcatg acctgcgtcg cctgcttgag gcggcgcatc cgccgcagct
     1981 gcccgacgag cgcgggcacg acgcggcggc tcccgggcat ccgccggtag g
//
LOCUS       DASZOB010000250         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_133903, whole genome shotgun sequence.
ACCESSION   DASZOB010000250 DASZOB010000000
VERSION     DASZOB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133903"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1013)
                     /locus_tag="VG709_04080"
     CDS             complement(129..1013)
                     /locus_tag="VG709_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWC32285.1"
                     /translation="MSASGGLTPQGHPAPVIEGDRRSRILGGGREGARSVTIAIASTI
                     VFVTVLVLVVVNSPGWEEVKRSFFNPVVFRESFPTIAERFLRNVQAFLIAEVLILVFA
                     LVIAVLRSLPGPVFFPVRALTIVYTDLFRGMPTILVIYILGFGAPALRLTGIPISPFF
                     WGIVALVLVDSAYGAEVYRAGIESGHPSQVASARSLGLTRWQSLRCVVLPQAVRRVIP
                     PLLNDFVGLQKDTALLALIGVLETFRQSQVDAAGSFNYTPYVATAMLFVLITIPLARF
                     TDWLIARERARRETGGRL"
     gene            complement(1021..1905)
                     /locus_tag="VG709_04085"
     CDS             complement(1021..1905)
                     /locus_tag="VG709_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWC32286.1"
                     /translation="MRRASAASLLAGVVLALAACGQPQQAAPQQPQQPDACEKDQLNL
                     VEPGVLTVGTDNPAYPPWFEGGTPEGSQWEINDPSTGEGFESAVAYAVADELGFSRDD
                     VQWVEVPFNQSFKPGEKDFDFDINQISYKPARDEAVDFSDSYYDVNQAVVSVEGSPIA
                     GATTIEELRSAKLGAPVGTTSYDVIQDVVQPEEDPGVYDSLNDAISALENGQIDGVVV
                     DLPTAFFIAAVQIDGGVIVGQFAITGEQEYFGMVFEEGNPLVECVNRALDRLKQDGTL
                     QTIQQEWLSDKVQAPVLQ"
     gene            complement(1902..2777)
                     /locus_tag="VG709_04090"
     CDS             complement(1902..2777)
                     /locus_tag="VG709_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32287.1"
                     /translation="MDPAPSAPRDLAQRVWGSWPSLPAGAAPAAAPERQSAPAAAPLE
                     PDVAELDIDEEQFDADLLRLEDRLRDTEDRLTSFAESMQQALRTLGDTIRASTAELPG
                     VVDRVVERRLNAVGDALLKLADLRAKLQEETAEAVKGMQASFQALEERLAETTQERVQ
                     ASIERLVSEVREVREDVARTTGSTAGEGRVLEAIEHRLRDRVAELPTRGDFASIRGAI
                     GTVASSLRGELAEAIERIGRIEQTLAGMASPAPERRADEAQEAAEPEAGPPPSQERSV
                     DDPFDGRPLFDFPER"
     gene            complement(2916..>3510)
                     /locus_tag="VG709_04095"
     CDS             complement(2916..>3510)
                     /locus_tag="VG709_04095"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HWC32288.1"
                     /translation="RGPASPAFRRPGVRGGERGLGLPVAGKPDSQDLCFVPGADPTAF
                     LERRAPHLVRDGEVVDGSGRIVGTHRGTFRFTVGQRRGIGVSTRDRVYVTDIDTSANR
                     VVVGPASLLARGGLVADRVTWVEGAPGGPFEADVKLRYRGSDVPAVVVPDGTRARVEF
                     RAPQAGVAPGQSVVFYAGDELLGGGRIALPLPAGTTV"
BASE COUNT          527 a         1276 c         1204 g          503 t
ORIGIN      
        1 cttccccgct ccggcggcgc cgatcagcgc gacgacctcg tgccgcgcga gcgcgaggtc
       61 gatccccttc agcacctcga gctcgccgaa cgacttgtgg acgtcctcga ggcgcagtgc
      121 ctccgcggtc acagccgccc gccggtctcg cgccgcgcac gttcgcgcgc gatgagccag
      181 tccgtgaacc gcgcgagcgg gatcgtgatc agcacgaaga gcatcgcggt cgcgacgtac
      241 ggcgtgtagt tgaacgaacc ggcggcgtcg acctgcgact ggcgaaacgt ctcgagcacg
      301 ccgatcaggg cgaggagcgc cgtgtccttc tgcaggccca cgaagtcgtt gagcagcggc
      361 gggatcacgc ggcggaccgc ctgcggcagc acgacgcagc gcagcgactg ccaccgcgtg
      421 aggccgagcg acctcgccga cgccacctgg ctcgggtggc ccgactcgat ccccgcccgg
      481 tagacctcgg cgccgtacgc cgagtccacg agcacgagcg ccacgatccc ccagaagaac
      541 ggcgagatcg ggatcccggt gaggcgaagc gccggcgcgc cgaagccgag gatgtagatc
      601 acgaggatcg tcggcatccc gcggaacagg tccgtgtaga cgatcgtgag ggcccgcacc
      661 gggaagaaca ccgggccagg caggctccga agcaccgcga tcacgagcgc gaacaccagg
      721 atcaagacct cggcgatgag gaacgcctgc acgttccgca ggaaccgctc cgcgatcgtc
      781 gggaacgact cacggaacac gaccgggttg aagaaagacc gcttgacctc ctcccatccc
      841 ggcgagttca cgacgacgag caccaggacg gtgacgaaca cgatcgtgct cgcgatcgcg
      901 atcgtgacgc tgcgcgcgcc ctcgcggccg ccgccgagga tgcgcgacct gcggtcgccc
      961 tcgatgaccg gcgccgggtg accctgaggc gtcagcccgc ccgaggcgct cacgccgtcg
     1021 ctactggagg accggcgcct ggaccttgtc ggagagccac tcctgctgga tcgtctggag
     1081 cgtcccgtcc tgcttcaggc ggtcgagcgc ccggttgacg cactccacga gcgggttgcc
     1141 ttcctcgaag accatcccga agtactcctg ctcgccggtg atcgcgaact gcccgacgat
     1201 cacgccgccg tcgatctgca ccgcggcgat gaagaaggcc gtcggtaggt cgacgacgac
     1261 gccgtcgatc tgtccgttct cgagcgcgga gatcgcgtcg ttcagcgaat cgtaaacgcc
     1321 gggatcctcc tcgggctgga cgacgtcctg gatcacgtcg tagctagtgg tcccgaccgg
     1381 ggccccgagc ttcgccgacc tgagctcctc gatcgtcgtc gcgcccgcga tcggcgatcc
     1441 ctcgacggac acgaccgcct ggttcacgtc gtagtagctg tcgctgaagt cgacggcctc
     1501 atcacgggcc ggcttgtacg agatctggtt gatgtcgaag tcgaagtcct tctcgccggg
     1561 cttgaaggac tggttgaacg gaacctcgac ccactggacg tcgtcgcggg agaaaccgag
     1621 ctcgtcggcg accgcgtacg cgacggcgct ctcgaacccc tcgccggtcg acgggtcgtt
     1681 gatctcccac tgcgacccct ccggggtgcc gccctcgaac cacggcggat acgccgggtt
     1741 gtcggtgccg accgtgagga cgcccggctc gacgagattg agctggtcct tttcgcacgc
     1801 gtccggctgc tgtggctgct gcggcgcggc ctgctgcggc tggccgcacg cggctaaggc
     1861 gaggacgacc ccggcgagaa gcgacgcggc ggacgccctc ctcatcgctc ggggaaatcg
     1921 aacagcgggc gcccgtcgaa ggggtcgtcg accgatcgtt cctgggacgg cggcggcccc
     1981 gcttccggct cggccgcctc ctgcgcctcg tcggcgcgcc gctcgggggc cggcgacgcc
     2041 atcccggcga gggtctgctc gatccgcccg attcgctcga tagcctccgc cagctctccg
     2101 cggagggacg aagcgaccgt cccgatcgcg ccccggatcg aggcgaagtc gcccctcgtc
     2161 gggagctcgg ccacgcggtc acggaggcga tgctcgatcg cctcgagcac ccgtccctca
     2221 ccggccgtgg agccggtcgt gcgcgcgacg tcctctcgca cctcgcgaac ctcggaaacc
     2281 agccgctcga tcgacgcctg cacgcgctcc tgcgtcgtct ccgcgagccg ctcctccaac
     2341 gcctggaacg acgcctgcat gcccttcacg gcctcggccg tctcctcctg cagcttcgcg
     2401 cgcaggtcgg cgagcttgag cagcgcgtca cccacggcgt tcagccgccg ttcgacgaca
     2461 cggtctacga caccgggaag ctctgcggtt gaggcgcgga tcgtgtcgcc gagcgtgcgg
     2521 agcgcttgct gcatcgactc ggcgaacgac gtgagccggt cctcggtgtc ccggagccgg
     2581 tcttcgagcc gcaggaggtc ggcgtcgaac tgctcctcgt cgatgtctag ctctgcgacg
     2641 tcgggttcga gcggcgccgc agcaggagcg gactgccgtt cgggcgccgc cgccggcgcg
     2701 gcccccgcgg ggaggctcgg ccacgatccc cacacgcgct gcgcgaggtc ccgcggcgcc
     2761 gaaggggccg ggtccaccgc gccctcgccc gacggtgcgt ccggccgctg acccgcctct
     2821 tcggaagccg gctctcggcg ctccgaagga ccattggggt gatcgtggtt cggcatcgtt
     2881 cttccttccg tgccgggggg cggcagactc aaaccttaca ccgtggttcc cgcaggaaga
     2941 gggagggcga tccttcctcc tccgaggagc tcatcgccgg cgtagaagac gacgctctga
     3001 ccgggtgcga cgccggcctg gggcgcgcgg aactccacgc gggcacgggt cccgtcgggc
     3061 acgacgacgg ccggtacgtc gcttccgcgg tacctgagct tcacgtcggc ctcgaagggg
     3121 ccgcctgggg caccctcgac ccacgtgacc cggtcggcca cgagcccgcc gcgggcgagg
     3181 agcgacgccg ggccgacgac cacgcggttg gcggaggtat cgatgtcggt cacgtacacg
     3241 cggtcccgcg tcgagacgcc gatgccgcgg cgctgtccga ccgtgaagcg gaacgtcccg
     3301 cggtgagtgc cgacgatgcg acccgacccg tcgacgacct ccccatctcg gacgaggtgc
     3361 ggagcccgtc gctcgaggaa cgcggtcggg tccgcgcccg ggacgaagca gaggtcctgc
     3421 gagtccggct tcccggcgac cgggagtccg agcccacgct cgccgcctcg gactcccggt
     3481 cgccggaaag ccggactcgc aggacctctg
//
LOCUS       DASZOB010000251         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_126261, whole genome shotgun sequence.
ACCESSION   DASZOB010000251 DASZOB010000000
VERSION     DASZOB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126261"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..753
                     /locus_tag="VG709_04100"
     CDS             154..753
                     /locus_tag="VG709_04100"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWC32289.1"
                     /translation="MADLRPLSRRFYARDPALVARDILGRLLVRETSGERLVGRIVEA
                     EAYATDDPASHSYRGETRRNATMFGPPAHAYVYVSHGIHHCLNVTTGHGNAVLLRAIE
                     PLEGLEWMVERRGVVDARLLCAGPGRLCEALGVGLADDGADLTTGEGLWLARGVPASA
                     VVATPRVGIRRAADVPWRFVVRGSRYASRPAGLRPRRPA"
     gene            complement(731..2935)
                     /locus_tag="VG709_04105"
     CDS             complement(731..2935)
                     /locus_tag="VG709_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HWC32290.1"
                     /translation="MTRPSPPFAGLLVTVVFTASCGLADVEVGPPDVRPLAQTTKVYD
                     AQGRLITELHGVEDRVLIGYRRIPQVMKDAVVAIEDQRYWHHRGIDLKALLRAAYSNL
                     ASGEIVQGGSTITEQFVKNRIVGPERSLGRKIREAAVAWRLEDRLSKEQILARYLNTV
                     YFGRGAYGIQRAAHTYFSRPATELRLHQAALLAGLIAAPSRWDPFERRAPARERRDFV
                     LSRMRDLGMISRDAHAHAVSKPLGLDPPRERNRYPAPWFIEYVKREILHDPRFGETYD
                     ERYRLLFEGGLRIHTTLDLDMQEAAERAVRGILYRSSDPYAGLTAVDPRTGEIKAMVG
                     GRDYFASPNVDPYSKVNLATGGTTGRQPGSAFKPFALVAALEAGIPPTATYPAPSFIA
                     IDDARCRNRGEPWTVSNYSDARYSGSMTLEQATIGSVNSVYAQIVEQIGPERVAEVAR
                     RMGIRSPLDPVCSLSLGSNEVNTLEMASAYGTLATNGRHTPTTGIARIEDARGGVIYR
                     WHPRLRQVVPRTAAWVATQILRKVVLQGTGRAANIGRPQAGKTGTNQLWRDAWFVGYI
                     PQFSAAVWVGHPSGQVSMASSRIGPVVGGSWPAQIWHAFMAAVTKRMPVRDFHRPAEA
                     EFVTVPIDVSRGCVATEATPQEDVRYIQFVPGTEPSEPCDEAPVASFAVVPSVVGLAA
                     SEAYATLQSAGFTVSQSTRTETSFPAGSVVSQSPDGGVEASSGSVVTLVVAA"
     gene            2985..>3333
                     /locus_tag="VG709_04110"
     CDS             2985..>3333
                     /locus_tag="VG709_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWC32291.1"
                     /translation="MLSVSRQLDLLLSGAADVIPEDELERKLERSVAEGAPLRVKLGI
                     DPSAPDLHLGHAVVLRTLRRFQDLGHTAVLIVGDFTGRVGDPSGQSETRPMLTGVELE
                     ENARTYLEQVRTVL"
BASE COUNT          471 a         1170 c         1183 g          509 t
ORIGIN      
        1 ccgtagacac cgactcgcac gccgtgccgc acctcgccgg gatgcggttc ggcgtggcga
       61 ccgcgcagcg cggttggctc acgaaggccg acgtcatcaa cacgtacccg ctggcgaagc
      121 tccggcgctt cctcgcgaag gggcgatagc ctcgtggcgg acctgcgccc gctctcgcgg
      181 cgcttctacg cgcgtgaccc cgcgctggtg gcgcgcgaca tcctcgggcg cctgctcgtc
      241 cgtgagacct ccggcgagcg gctggtcgga aggatcgtcg aggcggaggc ctacgcgacc
      301 gacgatcccg cgagccattc gtaccgcggc gagacgcgga ggaacgcgac gatgttcggc
      361 ccgccggccc atgcgtacgt ctacgtctct cacgggatcc accactgcct caacgtcacg
      421 acaggccacg gcaacgcggt cctgctccgc gcgatcgagc cgctggaggg gctcgagtgg
      481 atggtggagc gacgcggtgt cgtggacgct cgacttctct gtgcgggacc cggacgattg
      541 tgtgaggcgc tcggcgtcgg tctcgccgac gacggcgccg acctcacgac cggtgagggg
      601 ctgtggctgg cccgcggggt gccggcctcc gccgtcgtcg cgacgccacg ggtcggcatc
      661 cgccgcgccg ccgacgtccc gtggcggttc gtggtccggg ggagccgata cgcgtcgcgg
      721 cccgcgggtc tcaggccgcg acgaccagcg tgacgaccga gccggaggag gcctcgacgc
      781 cgccgtcggg cgactgactg acgacggagc cggccgggaa gctggtctcg gtgcgcgtcg
      841 actgcgatac cgtgaacccc gcactctgga gcgtcgcgta cgcctcgctc gcggcgagcc
      901 ccacgaccga cggcaccacg gcgaacgagg cgaccggtgc ctcgtcgcag ggctcgctcg
      961 gctccgtgcc agggacgaac tggatgtagc ggacgtcctc ctgcggcgtc gcctcggtcg
     1021 cgacgcagcc gcgtgacacg tcgatcggga ccgtcacgaa ctccgcctcc gcgggccggt
     1081 ggaaatcgcg gacgggcatg cgcttggtca cggccgccat gaaggcgtgc cagatctgcg
     1141 ccggccagct gcctccgacg accggcccga tgcgcgagct cgccatcgag acttggccgc
     1201 tcgggtgacc gacccagacc gctgcggaga actgcgggat gtagcccacg aaccacgcat
     1261 cgcgccagag ctggttcgtc ccggtcttcc ccgcctgcgg acggccgatg ttcgcggccc
     1321 ggccggttcc ctggagcacg accttccgga ggatctgcgt cgccacccac gccgccgtcc
     1381 gcgggacgac ttggcggagg cgcgggtgcc accggtagat cacgcctccc cgtgcatcct
     1441 cgatccgcgc gatgccggtc gttggggtgt ggcgcccgtt ggtcgccagc gtcccgtacg
     1501 cggaggccat ctcgagcgtg ttgacctcgt tcgacccgag cgagagcgag cacaccgggt
     1561 cgagtggact cctgatcccc atgcggcgcg cgacctcggc gacccgctcc ggtccgatct
     1621 gctcgacgat ctgggcgtag acggagttca cgcttccgat cgtcgcctgc tcgagtgtca
     1681 tcgagccgct gtagcgggcg tccgagtagt tcgagaccgt ccacggctcg ccgcggttcc
     1741 ggcatctcgc gtcgtcgatg gcgatgaacg agggagccgg gtaggtcgcg gtcggaggga
     1801 tgccggcctc gagcgcggcg acgagcgcga acggcttgaa cgccgacccg ggctgacggc
     1861 ccgtcgtccc accggtcgcg aggttgacct tcgagtacgg gtcgacgttc ggggacgcga
     1921 agtagtctcg cccgccgacc atcgccttga tctccccggt ccgtggatct accgcggtga
     1981 gccccgcgta cgggtcgctc gatcggtaga ggatgccgcg gaccgctcgc tccgccgcct
     2041 cctgcatgtc gaggtccagc gtcgtgtgga tcctgagccc gccctcgaac agcaggcggt
     2101 aacgctcgtc gtacgtctcg ccgaaccgcg ggtcgtgcag gatctcgcgc ttcacgtact
     2161 cgatgaacca tggagcgggg tagcggttgc gttcgcgagg cggatcgagt ccgagcggct
     2221 tcgagacggc atgcgcgtgc gcgtcgcggg agatcatccc gaggtcgcgc atccgcgaga
     2281 gcacgaagtc gcgccgctcg cgcgccggag cgcgccgctc gaacggatcc cagcgcgatg
     2341 gggccgcgat gagcccggcg aggagcgccg cctggtgcag ccggagctcc gtcgccggac
     2401 gcgagaagta cgtgtgagcc gcgcgctgga tcccgtacgc gccccggccg aagtagacgg
     2461 tgttgaggta cctcgcgagg atctgctctt tcgagaggcg atcctcgagt cgccacgcca
     2521 ccgcggcctc gcggatcttg cggccgagcg aacgctccgg gcccacgatg cggttcttga
     2581 cgaactgctc ggtgatcgtg gacccgccct ggacgatctc gcccgacgcg aggttcgagt
     2641 acgcggcccg cagcagcgcc ttgaggtcga tgcctcggtg gtgccagtac cgctggtcct
     2701 cgatcgcgac gaccgcgtcc ttcatgacct gcgggatccg ccggtagccg atcaggacac
     2761 ggtcctcgac gccgtgcagc tcggtgatga gccgcccctg cgcgtcgtag accttcgtgg
     2821 tctgcgcgag cgggcgcacg tccgggggtc cgacctcgac gtccgcgagg ccgcaggaag
     2881 ccgtgaacac cacggtcacg agaagcccgg cgaaaggcgg ggaaggccgg gtcacggcag
     2941 gagtatagcc tcggccttat gcgcccttgt tacgattcgc tcggatgttg tccgtctccc
     3001 gccagttgga tctcctcctc tctggcgccg ccgacgtcat cccagaggac gagctggagc
     3061 ggaagctcga gcgatccgtg gccgagggcg ccccgctccg cgtgaagctc gggatcgacc
     3121 cttccgcgcc cgatctccat ctcggccacg ccgtcgtgct acggacgctt cgccggttcc
     3181 aggacctcgg tcacaccgcg gtgctgatcg tcggcgactt caccggccga gtcggcgacc
     3241 cgagcggcca atcggagacg cggccgatgc tcacgggagt ggagctcgag gagaacgcgc
     3301 ggacctacct cgaacaggtc cggacggtgc tgg
//
LOCUS       DASZOB010000252         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_197467, whole genome shotgun sequence.
ACCESSION   DASZOB010000252 DASZOB010000000
VERSION     DASZOB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197467"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1199
                     /locus_tag="VG709_04115"
     CDS             63..1199
                     /locus_tag="VG709_04115"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HWC32292.1"
                     /translation="MADDMRFETRAIHVGQDPEPAFGAVSVPIYQTSTYVQDAVGKPK
                     AYDYARGGNPTRTALETVLASLEGARFGLAFASGMAAETTLLLLLKPGDHVLLPDDVY
                     GGTYRLLARVLADWGVAFDTVDMTDEAAFRKAVREETRVVWLETPSNPLLKVVDIRMA
                     ADIAHEVGARCVVDNTFATPYLQRPLELGADVVVHSVTKYLGGHSDLIGGAVTLNDDE
                     TAERLSFLQKAVGAVPGPMDCYLALRGVKTLAPRMDAHCRGARMVAEFLKSHAKVVRV
                     HYPGLMDDPGHEIAERQMRDYGGMVSFEVESEDEAVRVAEATRLFFLGESLGGVESLS
                     EVPGPMTHASVVGSPLEVSPALIRLSVGIEHPDDLVADLEQALG"
     gene            complement(1203..>2228)
                     /locus_tag="VG709_04120"
     CDS             complement(1203..>2228)
                     /locus_tag="VG709_04120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32293.1"
                     /translation="VALAGAFLRVWIAAGLLGGGAVGYVLLAFFRFDMAFQPLQVVFP
                     LLAAFLAFVAALGVRYFTEERERRRVTRVFGRYVAEDVVDEVLAAPERALATLEGAER
                     PISILFADLRGFTAASEGAAPKEVVGALNLYLDAMTRAVNEERGTIDKFMGDCVMAFW
                     GAPRPIFDHASKAVRAAQRMLDYIDEAKDRPEAGGLKVKGCGVGVATGVAVVGNIGSA
                     ERLDYTVIGDTVNTASRLCGVAGAGQVVVTEECAAALTDDFELAPLPPLTVKGKTKRL
                     TVFQVLREGQTAETFATGAVLDATEEKGHFEGEPEKEPQQVAPHRAASYAPVEPVDPT
                     IKPGPQK"
BASE COUNT          327 a          798 c          777 g          326 t
ORIGIN      
        1 gtgctgacgc gctcggacct cctcgagtac ctcgcccacc gccgccggac cggagggacg
       61 tgatggcaga cgacatgcgc ttcgagacga gggcgatcca cgtcgggcag gaccccgagc
      121 cggcgttcgg agcggtcagc gtcccgatct accagacgtc gacgtacgtg caggatgccg
      181 tcgggaaacc gaaggcctac gactacgcgc gcggcggcaa cccgacccgc acggcgctcg
      241 agaccgtcct tgcgtcgctg gagggcgcgc gcttcgggct cgcgttcgcg tcggggatgg
      301 cggcggagac gacgcttctc ctcctgctga agccgggtga ccacgtcctc ctgccggacg
      361 acgtctacgg cggtacctat cggttactcg cgcgcgtgct cgcggactgg ggcgtcgcgt
      421 tcgacacagt cgacatgacg gacgaggcgg cgttccgcaa ggccgtgcgc gaggagacgc
      481 gcgtcgtctg gctggagacg ccgtcgaatc cgctgctcaa ggtcgtcgac atccgtatgg
      541 cggccgacat cgcgcacgag gtcggagcgc gctgcgtcgt cgacaacacg ttcgcgacac
      601 cgtacctcca gcgcccgctc gagctcgggg ccgacgtcgt cgtgcactcg gtgacgaagt
      661 acctcggcgg ccactccgac ctcatcggcg gcgccgtcac gctgaacgac gacgaaacgg
      721 ccgagcgtct ctcgttcctc cagaaggcgg tcggggccgt gccgggcccg atggactgct
      781 acctcgccct acgcggcgtg aagacgctcg cgccgcgcat ggacgcgcac tgccgcggcg
      841 cgcggatggt cgccgagttc ctcaagtcgc acgcgaaggt cgtccgcgtc cactacccgg
      901 gcctcatgga cgaccccggg cacgagatcg ccgagcggca gatgcgcgac tacggcggga
      961 tggtgtcgtt cgaggtcgag agcgaggacg aggcggttcg cgtcgccgaa gcgacccggc
     1021 tcttcttcct cggcgagtcg ctcggcggcg tcgagtccct gagcgaggtc ccgggcccga
     1081 tgacgcacgc gagcgtggtc ggctcgccgc tcgaggtatc gccggcgctg atccgcttgt
     1141 cggtcgggat cgagcacccc gacgacctcg tcgccgacct cgagcaggcg ctcggctagt
     1201 cgctacttct gcgggccggg cttgatcgtc gggtcgacgg gttcgaccgg cgcgtagctc
     1261 gcggcgcggt gaggcgcgac ctgctggggc tccttctccg gctcgccctc gaagtggccc
     1321 ttctcctcgg ttgcgtcgag caccgcgccg gtcgcgaacg tctcggcggt ctgcccttca
     1381 cgcagcacct ggaagacggt gagccgcttg gtcttcccct tgacggtgag cggtggcagc
     1441 ggcgcgagct cgaagtcgtc ggtgagcgcg gcggcgcact cctctgtgac gacgacctgc
     1501 cccgcgccgg cgacgccgca gagccggctc gcggtgttca cggtgtcccc gatcaccgtg
     1561 tagtccagcc gctcggccga gccgatgttg ccgacgacgg cgacgccggt cgcgaccccg
     1621 acgccgcagc ccttgacctt cagccctccg gcctcgggcc tgtccttcgc ctcgtcgatg
     1681 tagtcgagca tccgctgcgc cgcgcggacc gccttgctgg catggtcgaa gatcgggcgc
     1741 ggcgctcccc agaacgccat gacgcaatcg cccatgaact tgtcgatggt ccctcgctcc
     1801 tcgttcaccg cacgcgtcat cgcgtcgaga tagaggttga gcgccccgac gacctccttc
     1861 ggggcggcgc cctccgaggc ggcggtgaag ccgcgcaggt ccgcgaacag gatcgagatc
     1921 ggccgctcgg ctccctcgag cgtggcgagc gcgcgttcgg gcgcggcgag gacctcgtcg
     1981 acgacgtctt cggcaacgta gcgcccgaag acgcgcgtca cgcggcgccg ctcgcgctcc
     2041 tcggtgaagt agcggactcc gagcgccgcg acgaacgcga ggaacgcagc gagcagcggg
     2101 aacaccacct ggagcggctg gaacgccata tcgaaccgga agaacgccag cagcacgtac
     2161 cccaccgcgc cgccaccgag gaggcccgcg gcgatccaga cgcgcagaaa cgcgccggcc
     2221 aacgcgac
//
LOCUS       DASZOB010000253         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_55867, whole genome shotgun sequence.
ACCESSION   DASZOB010000253 DASZOB010000000
VERSION     DASZOB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55867"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VG709_04125"
     CDS             complement(<1..728)
                     /locus_tag="VG709_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWC32294.1"
                     /translation="MWLIALVVVLVIFGSLLAMAEASMTRMTRVRALALEDEGRRHST
                     RLVQIESDPPRYLNAIYLWVMLAQNGSAILVAIIAERTLGNIWITIISVAFTLLYFVI
                     VEAMSKTFAVLHSDRVALALSPTVYVLSQILRWPTRFLIGLANILLPGRGLRSGPFVS
                     EEEILAMAEAGHEEGAIEEGEKELIHSIFEFGDTIVREVMTPAPDIVGVDVEQPLDSV
                     LELVIRHGYSRIPVYKGSINEVVG"
     gene            complement(728..1162)
                     /gene="ybeY"
                     /locus_tag="VG709_04130"
     CDS             complement(728..1162)
                     /gene="ybeY"
                     /locus_tag="VG709_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWC32295.1"
                     /translation="MDDDGSQPHVALSDRQDLPVDGDALADLARSTLVAEGVRSGELS
                     LSFVTGDEIADLHQRYMDEPGPTDVLSFPQDDPRAAEQPRLIGDVVVCPRYALEDGRD
                     LEPELRLLVVHGVLHLLGYDHEAEEERRAMWERQERYAGMRP"
     gene            complement(1169..>1606)
                     /locus_tag="VG709_04135"
     CDS             complement(1169..>1606)
                     /locus_tag="VG709_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWC32296.1"
                     /translation="LYEKIDPYMKPLYDALYEMMDADALQRLIERGTVEVAPLGYMRG
                     RTLNDSFIILDEAQNTSPEQMKMFLTRLGFGSKAAVNGDVTQTDLPAGQRSGLAVVEE
                     ILRGIEGIEFIHLGARDVVRHRIVQDIVEAYRRHTEVVASDRS"
BASE COUNT          264 a          541 c          552 g          249 t
ORIGIN      
        1 atgccgacga cctcgttgat cgatcccttg tagacgggga tcctcgagta gccgtgacgg
       61 atcacgagct cgaggacgga gtcgagcggc tgctccacgt cgacgccgac gatgtcgggc
      121 gccggcgtca tcacctcgcg cacgatcgtg tcgccgaact cgaagatcga gtggatcagc
      181 tccttctcgc cctcctcgat cgccccctcc tcgtggccgg cctcggccat cgcgaggatc
      241 tcctcctccg agacgaacgg accgctgcgc agcccgcgcc cgggaagcag gatgttcgcg
      301 agcccgatga ggaagcgcgt gggccagcgc aggatctgcg agaggacgta caccgtcggc
      361 gagagcgcga gcgccacgcg gtcggagtgg agcacggcga acgtcttcga catcgcctcg
      421 acgatcacga agtacagcag cgtgaacgca accgagatga tcgtgatcca gatgttgccg
      481 agcgtccgct cggcgatgat cgcgacgagg atcgcggacc cgttctgcgc gagcatcacc
      541 cagaggtaga tcgcgttgag gtagcgcggc gggtcggact cgatctggac gagccgtgtg
      601 ctgtggcgac ggccctcgtc ctcaagcgcg agcgcgcgga cgcgcgtcat ccgcgtcatc
      661 gaggcctcgg ccatcgcgag cagcgacccg aagatgacga gcacgacgac gagggcgatg
      721 agccacatca cggccgcatg cctgcgtacc gctcctgccg ctcccacatc gcgcgacgct
      781 cctcctccgc ctcgtggtca tacccaagga gatggagcac gccgtggacg acgagcagcc
      841 ggagctccgg ctcgaggtcg cgcccgtcct cgagcgcgta tcgcgggcac accacgacgt
      901 cgccgatgag gcgcggctgc tcggccgcgc gggggtcgtc ctgcgggaac gacagcacgt
      961 ccgtggggcc cggctcgtcc atgtagcgct ggtgcaggtc ggcgatctcg tcgcccgtca
     1021 cgaacgagag cgagagctct cccgagcgca cgccctcggc aacgagggtc gaacgggcga
     1081 ggtcggcgag cgcgtcaccg tcgaccggca ggtcctgccg atcggatagc gcgacgtgcg
     1141 gttgactgcc gtcgtcgtcc acggcggctc aggagcggtc ggaggccacg acctcggtgt
     1201 ggcgccgata ggcctcgacg atgtcctgga cgatgcggtg acggaccacg tcgcgcgccc
     1261 cgaggtggat gaactcgatc ccctcgatgc cgcgcaggat ctcctcgacg acggcgagcc
     1321 ccgaccgttg cccggccggc aggtcggtct gggtgacgtc gccgttgacc gcggccttgg
     1381 agccgaaccc gagccgggtc aggaacatct tcatctgttc gggagaggtg ttctgcgcct
     1441 cgtcgaggat gatgaacgag tcgttgagcg tccgtccgcg catgtagccg agcggcgcca
     1501 cctcgacggt gccccgctcg atcagccgct ggagcgcgtc tgcgtccatc atctcgtaca
     1561 gcgcgtcgta caacggcttc atgtacgggt cgatcttctc gtagag
//
LOCUS       DASZOB010000254         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_185404, whole genome shotgun sequence.
ACCESSION   DASZOB010000254 DASZOB010000000
VERSION     DASZOB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185404"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VG709_04140"
     CDS             <1..235
                     /locus_tag="VG709_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32297.1"
                     /translation="NQPFGGCADETAPYRAMCPTRSADMCIHCGLATPNGLGDVCVAC
                     AVAVRSEVRRGLDAIEKYLDGWSELDRWRSDQE"
     gene            complement(284..>1501)
                     /locus_tag="VG709_04145"
     CDS             complement(284..>1501)
                     /locus_tag="VG709_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32298.1"
                     /translation="DWMRLWTAVHEMGHAFNLAHSWQKTLGTAWIPLANEPNARSFMN
                     YPFNVAGGQAAFFANFEYRFSDQELLFIRHAPERFVQMGNAAWFDHHGFEEAAVSEGP
                     TLALELRVNREQARYEFMEPVWLELKLTNVSEQPRLVDADVLDADSLTLILKKHGQEA
                     RRLVPFRQKCVQPETKALMPGESIYGTVLASAGLNGWDVAEPGTYLVQAAAHVGDEDV
                     VSAPFEVRIAPPLSHEEEYSAGDVFTQETARVLVFGGSRYLEGANDVLREVVERFPDR
                     RIALHARVALGNPLTLDYKLVTPTDDGVEVEVERADPDEAAELIEPALVERAGVAAES
                     FGHIRYRGIAERVARRLATKGAEDEATRTIDSAIDTLAERTVRGRPVKGEVIEDLKET
                     REAIVSGKTKATA"
BASE COUNT          206 a          547 c          504 g          244 t
ORIGIN      
        1 gaaccagccg ttcgggggat gtgcggacga gacggccccg tatcgtgcga tgtgccccac
       61 ccgttctgcg gacatgtgca tccactgcgg tcttgctacg cccaacggcc tgggcgacgt
      121 atgcgtcgcc tgcgcggtgg cggtccgctc cgaggtccgc cgcgggctgg acgcgatcga
      181 gaagtacctc gacggctggt cggagctcga ccgctggcgc agcgaccagg agtgaggacg
      241 gccccgacgc ggggccggag ccgtcctcct agccgagcga ggcctaggcc gtcgccttcg
      301 tcttgccgga gacgatcgcc tcgcgcgtct ccttgaggtc ctcgataacc tcgcccttga
      361 ccggccgccc gcgcaccgtc cgctcggcga gggtgtcgat cgccgagtcg atcgtcctcg
      421 tcgcctcgtc ctcggcaccc ttggtcgcga gcctccgtgc gacccgctcg gcgatcccgc
      481 ggtagcggat gtgtccgaac gactcggcag ccacgccggc ccgctcgacg agcgccggct
      541 cgatcagctc cgccgcctcg tcggggtccg cccgctcgac ctcgacctcc acaccgtcgt
      601 cggtcggcgt cacgagcttg tagtcgaggg tcagcgggtt cccgagcgcg actcgcgcgt
      661 gcagcgcgat ccgccggtcg gggaaccgct cgacgacctc ccggaggacg tcgttcgcgc
      721 cctccagata gcggctgccg ccgaagacga gcacgcgagc cgtctcctgc gtgaagacgt
      781 cgcccgccga gtactcctcc tcgtgcgaga gcggcggggc gatccggacc tcgaacgggg
      841 ccgagacgac gtcctcgtcg ccgacgtgcg ctgccgcctg gaccaggtac gtgcccggct
      901 cggcgacgtc ccagccgttg agccccgccg aggcgagcac ggttccgtag atcgattcgc
      961 ccggcatgag cgcctttgtc tccggctgca cgcacttctg ccggaacggg acgagccgcc
     1021 gagcctcctg gccgtgcttc ttgaggatca gcgtgagcga gtcggcgtcg agcacgtccg
     1081 cgtcgacgag ccgcggctgc tcgctcacgt tcgtcagctt gagctcgagc cagacgggct
     1141 ccatgaactc gtagcgcgcc tgctcgcggt tcacgcggag ctcgagcgcg agcgtcggtc
     1201 cttcggacac ggccgcttcc tcgaacccgt ggtggtcgaa ccaggcggcg tttcccatct
     1261 gcacgaaccg ctccggcgcg tgccggatga acagcagctc ctggtcgctg aaccggtact
     1321 cgaagttggc gaagaacgcc gcctgcccgc ccgccacgtt gaacggatag ttcatgaagc
     1381 tccgcgcatt gggctcgttc gcgagcggga tccacgcggt gccgagcgtc ttctgccacg
     1441 aatgggcgag gttgaacgcg tgccccatct cgtgcaccgc cgtccagagc cgcatccagt
     1501 c
//
LOCUS       DASZOB010000255         3803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_146661, whole genome shotgun sequence.
ACCESSION   DASZOB010000255 DASZOB010000000
VERSION     DASZOB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146661"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VG709_04150"
     CDS             <1..906
                     /locus_tag="VG709_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWC32299.1"
                     /translation="FWGNEEVNSGGGVWYPPAVDADSGMTFWGTGNPAPFPGIVDFPN
                     AESRPGPNLYTNSIVALQGASGDLEWFKQIKPRDLFDLDFQTPRILATIEGEDGAREI
                     VIGSGKLGYVVAFDTESGDILWSTRVGEHENDELARVPEGETIRVFPGVLGGVETPMA
                     LAEDVLYVPVLDLPTPYTADGHGAEDGTEAVTNAEAETDFSKAKSHVAALELHTGRVL
                     WKSDEFDEALFSGVTVVNDLLLAATYDGTMYALERETGEKVWSEQAPAGINAWPSVAG
                     DTIVWPAGVGQKPRLVALSLSAGGA"
     gene            926..1399
                     /locus_tag="VG709_04155"
     CDS             926..1399
                     /locus_tag="VG709_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWC32300.1"
                     /translation="MTMLHEFGAMKRTVMLFAVILAAGLLAACGGDGDTGQVGSPSPT
                     AETPANGDDGDDTDGGAAAQLELVAEGNSFNQDQLEASSGASVELTLQNNDSVPHNFS
                     LYESEAAEENIFAGETFSGPDASRTYEFEAPDDAGTYFFRCDVHPTTMTGDFVVT"
     gene            1466..3226
                     /locus_tag="VG709_04160"
     CDS             1466..3226
                     /locus_tag="VG709_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWC32301.1"
                     /translation="MAKIASELLVERLIAWGVDTVFGLPGDGINGIMEGLRRHKDRIR
                     FVLVHHEEAAAFMASAYAKSTGRLGVCLATSGPGGIHLLTGLYDAKMDHQPVLAITGM
                     QSTTQLGTGFQQEVHLEKVFDDVTEYNALVNVPVQIPTLVDIAVRTALSRRGVSHLTF
                     PVDMQESDADTNPYEGGLGTARPPMTAPIYRPPRVVPQEDDLRRGADVLNAGTNVVML
                     VGAGARGAGEEVLAVAETLGAPIVKTLRGKDVLPDDHPLTTGGLGLLGTLPSEDAMEK
                     ADTLFMVGTNFPYTTYLPGEARTVQIDLEAMRVGNRQPVEVPLVGDAKNTLDALAPHL
                     RRKEDRRFLEDRQEQMREWRENMAALEDPEREPIQPQYLARLIDRHASDDAILCTDSG
                     TAATWAARHFDIRGDRRFYLSGNLATMAPGLPYAIASQWAHPGRQCIAFVGDGAFAML
                     MAEFNTATRYELPIKVIILNNRSLGQILWEQMALGYPEHGVRWERSFDFAPWAESCGG
                     RGIRIEKPGDLESAVQEAFGYPGPALVDVVVNPDEPPMPAKIEYDKAKGFAEAFLKGQ
                     PRRAAIASTVFRDKIEQLKA"
     gene            3228..>3803
                     /locus_tag="VG709_04165"
     CDS             3228..>3803
                     /locus_tag="VG709_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HWC32302.1"
                     /translation="MATRRPDAAVERVDVDAYTVPTDAPEADGTLTWDQTTVVVVHVS
                     AGGASGIGFTYGPSACATLVRDALASVVVGLDAMDVPAAWAQMVQAIRNIGRPGVASM
                     AIAAVDTALWDLKARLLDISLATLLGRVRTEVPVYASGGFTSYREDDLGSQLGTWVHD
                     WGIPRVKMKVGTGWGTEPERDVERVRVAREAV"
BASE COUNT          617 a         1265 c         1404 g          517 t
ORIGIN      
        1 ttctggggca acgaggaggt caacagcggg ggaggcgtct ggtacccgcc ggccgtcgac
       61 gcggattccg gcatgacgtt ctgggggacc ggcaacccgg ctccgttccc gggcatcgtc
      121 gacttcccca acgccgaaag ccggccgggg ccgaacctct acaccaactc gatcgttgcg
      181 ctccagggcg cgagcggtga cctcgagtgg ttcaagcaga tcaagccgcg ggatctcttc
      241 gacctcgatt tccagacgcc gcggatcctc gcgacgatcg agggcgagga cggggcccgg
      301 gagatcgtga tcggctcggg gaagctcggg tacgtcgtcg cgttcgacac ggagagcggc
      361 gacatcctgt ggagcacgcg cgtcggcgag cacgagaacg acgagctcgc gcgcgtcccc
      421 gaaggcgaga cgatccgggt gttcccgggg gtcctcggcg gcgtcgagac tccgatggcg
      481 ctcgccgagg acgtcctcta cgtgcccgtc ctcgacctac cgaccccgta caccgccgac
      541 ggccacggcg ccgaggacgg tactgaggcg gtcacgaacg ccgaggccga gaccgatttc
      601 agcaaggcga agagccacgt cgcggccctc gagctccaca cggggcgggt gctgtggaag
      661 agcgacgagt tcgacgaggc gctgttcagc ggcgtgacgg tcgtgaacga cctcctgctc
      721 gcggcgacgt acgacggcac catgtacgcg ctcgagcgcg agaccggcga gaaggtgtgg
      781 tcggagcagg cgcctgcggg tatcaacgca tggccttcgg tggccggcga cacgatcgtc
      841 tggccggcgg gcgtggggca gaagccgcgg ctcgtcgcac tgagcctctc ggccggcggc
      901 gcgtgaccga caacgacgag gagcgatgac gatgctgcac gagttcggag ccatgaagag
      961 gaccgtgatg ttgttcgcgg tgatcctcgc ggccggcctc ctggccgcct gcggaggtga
     1021 cggggacacg gggcaggtcg gctctccgtc cccgaccgcc gagacgccgg cgaacggcga
     1081 cgacggcgac gacaccgacg gcggtgcggc ggcacagctc gagctcgtcg ccgaggggaa
     1141 cagcttcaac caggaccagc tcgaggcctc gagcggtgcg agcgtcgagc tgacgctcca
     1201 gaacaacgac tcggtgccgc acaacttctc gctgtacgag tccgaggcgg ccgaggagaa
     1261 catcttcgcg ggcgagacct tcagcggccc cgatgcgtcg cgcacgtacg agttcgaagc
     1321 gccggacgac gcggggacgt acttcttccg gtgcgacgtg cacccgacga cgatgacggg
     1381 tgacttcgtc gtgacgtgac cgacccgagc cgcgtagggc gtcgcaggct tgggtaggtc
     1441 accgataagc gacagaggag acgccgtggc gaagatcgcg tccgagctgc tggtcgagcg
     1501 cctgatcgcg tggggggtcg acaccgtctt cgggctccct ggcgacggga tcaacgggat
     1561 catggagggg ctccgccgcc acaaggaccg gatccggttc gtgctcgtgc accacgagga
     1621 ggcggcggcg ttcatggcga gcgcgtacgc gaagtcgacg ggacgcctcg gcgtctgcct
     1681 cgcgacctcg gggcccgggg gcatccacct gctcaccggg ctctacgacg cgaagatgga
     1741 ccaccagccg gtgctcgcga tcaccgggat gcagtcgacg acccagctcg ggacggggtt
     1801 ccagcaggag gtccacctcg agaaggtgtt cgacgacgtc accgagtaca acgcgctggt
     1861 caacgtgccg gtccagatcc cgacgctcgt cgacatcgcg gtgcgcacgg ccctgtcgcg
     1921 ccggggggtc tcccacctca cgttccccgt cgacatgcag gagtccgacg ccgacacgaa
     1981 cccgtacgag gggggcctcg ggaccgcgcg gcctccgatg accgccccga tctaccgccc
     2041 gccgcgcgtc gtcccgcagg aggatgacct ccggcggggc gcggacgtac tcaacgcggg
     2101 gacgaacgtc gtgatgctgg tcggcgccgg cgcccgcgga gccggcgagg aggtcctcgc
     2161 cgtggcggag acgctcggcg cgccgatcgt gaagacgctg cgcggcaagg acgtcctccc
     2221 cgacgatcac ccgctgacga cgggcggtct cggcctgctc gggaccctgc cctcagagga
     2281 cgcgatggag aaggccgaca cgctcttcat ggtcgggacc aacttcccgt acacgacgta
     2341 cctcccgggc gaggcacgga cggtgcagat cgacctcgag gccatgcgcg tagggaaccg
     2401 gcagccggtc gaggtgccgc tggtcggcga cgcgaagaac acgctcgacg ccctcgcgcc
     2461 gcacctccgc aggaaggagg accggcggtt cctcgaggac cggcaggagc agatgcgcga
     2521 atggcgcgag aacatggcgg cgctcgagga tcccgagcgc gagccgatcc agccgcagta
     2581 cctggcccgg ctgatcgacc ggcatgcgtc tgacgacgcg atcctctgca ccgactcggg
     2641 gacggcggcg acgtgggccg cgcgccactt cgacatacgg ggcgaccggc gcttctacct
     2701 gtccgggaac ctcgccacga tggcgcccgg tctcccgtac gcgatcgcct cgcagtgggc
     2761 gcacccggga cgccagtgca tcgcgttcgt cggcgatggc gcgtttgcga tgctgatggc
     2821 ggagttcaac acggcgacgc ggtacgagct cccgatcaag gtcatcatcc tcaacaaccg
     2881 ctcgctgggc cagatcctgt gggagcagat ggcgctcggc tacccggagc acggcgttcg
     2941 ctgggagcgc agcttcgact tcgcaccgtg ggcggagtcc tgcggcggcc gcgggatcag
     3001 gatcgagaag ccgggcgacc tcgagagcgc cgtccaagag gcgttcgggt acccgggtcc
     3061 ggcgctggtc gacgtcgtcg tgaacccgga cgagccgccg atgcccgcca agatcgagta
     3121 cgacaaggcg aaggggttcg cggaggcgtt cctcaagggg caaccgcggc gcgccgcgat
     3181 cgcctcgacg gtgttccgcg acaagatcga gcagctgaag gcgtgaggtg gccacccggc
     3241 ggccggatgc cgcggtagag cgcgtcgacg tcgacgcgta cacggtcccg accgacgcgc
     3301 ccgaggccga cggcacgctt acgtgggatc agacgacggt cgtggtcgtc cacgtgtcgg
     3361 ccggcggtgc gtcggggatc gggttcacgt acgggccgtc ggcctgcgcc acactcgttc
     3421 gcgacgccct cgcgtcggtc gtcgtcgggc tcgacgcgat ggacgtcccg gcggcgtggg
     3481 cgcagatggt gcaggcgatc cggaacatcg ggcgccccgg agtggcctcg atggcgatcg
     3541 ccgcggtcga caccgcgctg tgggacctga aggcgcggct gctggacatc tcgctcgcga
     3601 cgctcctcgg ccgcgtccgg accgaggtgc cggtctatgc gagcggcggg ttcacgtcgt
     3661 accgcgagga cgacctgggc tcgcagctcg gcacgtgggt ccacgactgg gggatcccac
     3721 gcgtgaagat gaaggtggga acgggctggg ggaccgagcc cgagcgcgac gtggagcggg
     3781 tccgggtggc ccgcgaggcc gtc
//
LOCUS       DASZOB010000256         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_156781, whole genome shotgun sequence.
ACCESSION   DASZOB010000256 DASZOB010000000
VERSION     DASZOB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156781"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VG709_04170"
     CDS             complement(<1..745)
                     /locus_tag="VG709_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWC32303.1"
                     /translation="MADAPAIEVTDLTVAYGDVPVLWDVDLTVPTGVLMAIVGPNGAG
                     KTTLVKAVLGLVRPAAGQVLVSGRPYVAGRSGVAYVPQRGTVDWDFPTSVLDVVMMGR
                     YGALGWIRRPGRRERERALDALAKLGIDALADRQISQLSGGQQQRVFLARALVQDADV
                     YVMDEPFQGVDATTERAVVELLRSLRAERKTVVAVHHDLQTVPEYFDWVTLLNVRRVA
                     SGPVGEVFTEENLRLCYGAPLAFLRPNGGP"
     gene            complement(738..1679)
                     /locus_tag="VG709_04175"
     CDS             complement(738..1679)
                     /locus_tag="VG709_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWC32304.1"
                     /translation="MSKFLTAAVLAAAMASVGCGTPVAATEAPDLSGRTLRVVATTSM
                     VADLVTQVAGDRATVTGLMGSGIDPHGYKASERDVMTLAEADVVFYNGLHLEAKMSDV
                     FERVEGRIVTAAVTDGLPRDRLVHSPQFTGAYDPHVWFDVSLWREAASEVADVLAGVD
                     PAHADVYARNAEAYRGELARLDEDVRALVASVPRERRVLVTAHDAFGYFGRAYGFEVH
                     GLQGISTVAEAGAADVQRLADFIAERRIPAVFVESSVPQQTIRAVQEAVGSRGFEVEI
                     GGQLFSDAMGDPGTPEGTYPGMVLHNVRTIADALRDG"
BASE COUNT          279 a          669 c          674 g          247 t
ORIGIN      
        1 cggggccacc gttcgggcgg aggaacgcga ggggcgcgcc gtagcagagg cggaggttct
       61 cctcggtgaa cacctcgccg accgggccgc tcgcgacgcg acggacgttc agaagcgtca
      121 cccagtcgaa gtactcgggc acggtctgga ggtcgtggtg cacggcgacg accgtctttc
      181 gctccgcgcg gagcgagcgg aggagctcca cgaccgcgcg ctcggtcgtc gcgtcgacgc
      241 cctggaacgg ctcgtccatc acgtacacgt cggcgtcctg gacgagcgcg cgggcgagga
      301 agacgcgctg ctgctgcccg cccgagagct ggctgatctg acggtccgcg agcgcgtcga
      361 tcccgagctt cgcgagcgcg tcgagcgcgc gctcgcgctc gcgccgcccg ggccgacgga
      421 tccacccgag cgcgccgtag cggcccatca tgacgacgtc gaggacgctc gtcggaaagt
      481 cccagtccac cgtgccgcgc tggggcacgt aggcgacgcc gctgcgcccg gccacgtacg
      541 gccgcccgct cacgaggacc tgtccggccg cggggcggac gaggccgagg accgccttca
      601 ccagcgtcgt cttgccggcg ccgttcgggc cgacgatcgc catgagaacg ccggtcggga
      661 cagtgaggtc gacgtcccag agcacgggga cgtcgccgta cgcgacggtc aggtcggtca
      721 cctcgatcgc gggagcgtca gccatcgcgg agcgcgtccg cgatcgttcg gacgttgtgc
      781 agcaccatcc cgggatacgt cccctcgggc gttccgggat cgcccatcgc gtccgagaag
      841 agctggccgc cgatctcgac ctcgaagccg cggctcccaa cggcctcctg cacggcgcgg
      901 atcgtctgct gcggaacgga cgactccacg aagacggcgg gaatgcgccg ttcggcgatg
      961 aagtcggcga gacgttgcac gtcggccgca ccagcctcgg cgaccgtcga gatgccctgg
     1021 aggccgtgga cctcgaaccc gtacgcgcgg ccgaagtacc cgaacgcgtc gtgggccgtg
     1081 accaggacgc gacgctcgcg tggcacggac gcgacgagcg ctcgcacgtc ctcgtcgagc
     1141 cgggcgagct ccccgcggta ggcctccgcg ttccgcgcgt acacgtcggc gtgcgccgga
     1201 tccacgccgg cgagcacgtc ggcgacctcg ctcgcggcct cccgccagag cgacacgtcg
     1261 aaccagacgt gcgggtcgta cgcaccggtg aactgcggcg agtggacgag ccgatcgcgc
     1321 ggcagcccgt cggtcaccgc cgcggtcacg atccggccct cgacgcgctc gaagacgtcg
     1381 ctcatcttcg cctccaggtg gaggccgttg tagaagacga cgtcggcctc ggcgagcgtc
     1441 atgacgtcgc gctcgctcgc cttgtagccg tgggggtcga tcccactgcc catcaggccc
     1501 gtcacggtcg cgcggtcgcc cgcgacctgc gtgacgaggt ccgccaccat gctcgtcgtc
     1561 gcgacgactc tgagcgtccg gccggacagg tcgggcgcct ccgtcgcggc caccggcgtg
     1621 ccgcacccga cgctcgccat cgcggccgcg agtaccgccg ccgtcagaaa cttagacacg
     1681 tctaaacctt agtttcggcg cctcgcggtg cgcaagagta acggtacggg gcgacggtcg
     1741 ggatagagtt cgccgcgatg ggacgcgggt ctcgcccgat acggcggtgg cgcaacgacc
     1801 gccagcgaaa gaagacggag cgggccaagc gggatgcggg tcggcgccgg cggggtgccg
     1861 cctagatgg
//
LOCUS       DASZOB010000257         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_43171, whole genome shotgun sequence.
ACCESSION   DASZOB010000257 DASZOB010000000
VERSION     DASZOB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43171"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VG709_04180"
     CDS             complement(<1..314)
                     /locus_tag="VG709_04180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC32305.1"
                     /translation="MREPERRTLEGQIEYYRRRAQTGSDPWITRAVREEATYAPDDAL
                     GPEESEALRALEELDLRGNILELACGTGAWTEHLVPHASRVTAVDASPEILEVNRARV
                     GDP"
     gene            360..1703
                     /gene="tig"
                     /locus_tag="VG709_04185"
     CDS             360..1703
                     /gene="tig"
                     /locus_tag="VG709_04185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWC32306.1"
                     /translation="MQTSLEHVGKHTVRVSVEVGPDEAKPILDVSFRHLADGVNVPGF
                     RKGKAPRKVIEAQVGRGAVLQEFLEHALPEFYFAAVRDLELAPIGDPEFDDLDVADVE
                     RQGLRFTATVDVRPRLEWTREDYRGLSVERPPAEVSNREVDEQLDRLRERFAELDVVG
                     RPARRGDYVVADIRAYVHDQEIPEASGQDVLYEVGGEQLAPELDKELEGTRSGDIVRA
                     NARLPEGLGEHGGKEIAFQALVKEVKSKRLPAVDDDFAKTASEFDTLDELRADVREKV
                     AALKHAAADGAVRDEALRALAGKVDVELPERLVDGETEARVERARQRAEQQGLKLEQV
                     LDAQRIDELQFRSDARAHATRAIQADLALEAVARAEDLRATDEDIDRAIEVIARDVGR
                     SANDVRRTLEQTGQITSLAGDIIRDKALDVVVEHANVVEEGGKGSGSPASEEGKP"
     gene            1700..2329
                     /locus_tag="VG709_04190"
     CDS             1700..2329
                     /locus_tag="VG709_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWC32307.1"
                     /translation="MKNYLVPIVIEQTNRGERSFDIYSRLLKERIVFLGTPIEDQVAN
                     LVMAQLLHLESEDPEKDINIYINSPGGDITSLFAIYDTMQYIKPDVSTIVMGQAASAA
                     AVLLAAGTKGKRYALPHSRVLIHQPHGQAGGQAVDIEIQAKEILRYRSLLDELLAEHT
                     SQPLEKVKHDTDRDFIMTSEQAKEYGIIDEGISSRKLRVELTAAGDGKG"
     gene            2368..>2635
                     /locus_tag="VG709_04195"
     CDS             2368..>2635
                     /locus_tag="VG709_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HWC32308.1"
                     /translation="MAKFGDSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNEI
                     IEEELSETTELKLEELPKPREIYKFLNDYVIGQEQAKKVLSVAVY"
BASE COUNT          485 a          863 c          903 g          384 t
ORIGIN      
        1 ggatcgccga ccctcgcgcg gttgacctcg aggatctccg gcgaggcgtc gaccgcggtg
       61 acccggctgg cgtgtggaac gaggtgctcc gtccaggcgc cggtaccgca ggcgagctcg
      121 aggatgttgc cacggaggtc gagctcctcg agcgcgcgaa gggcctcgct ttcctcggga
      181 ccgagggcgt cgtcgggcgc gtacgtcgcc tcttcgcgta cggcgcgagt gatccatggg
      241 tcggagccgg tctgcgcccg acgccggtag tactcgatct gtccctcgag cgttcggcgt
      301 tccggctcac gcaccatggg gaagcgtagt tcgccgccca tataatcgcc cgaccttcga
      361 tgcagacctc tctcgaacac gtcggcaagc acaccgtgag ggtctcggtc gaggtcggcc
      421 ccgacgaggc caagccgatc ctcgatgtct cgttccgcca cctcgcggac ggcgtcaacg
      481 tgcccgggtt ccggaagggc aaggcgccac gcaaggtcat cgaggcgcag gtcgggcgcg
      541 gagccgtgct gcaggagttc ctcgagcacg cgctgccgga gttttacttc gctgctgtcc
      601 gcgacctcga gctcgcaccg atcggcgatc cagagttcga cgacctcgac gtcgccgatg
      661 tcgagcgcca gggcctccga ttcacggcca ccgtcgacgt ccgaccgcga ctcgagtgga
      721 cccgggagga ctaccggggc ctgagcgtcg agcgtcctcc cgccgaggtg tcgaatcgcg
      781 aggtcgacga gcagctcgac cggttgcgcg agcggttcgc cgagctcgac gtcgtcggcc
      841 gacccgcgag acgcggcgat tacgtcgtcg cggacatccg cgcgtacgtg cacgaccagg
      901 agatcccgga ggcttcgggc caagacgtcc tctacgaggt cggcggcgag cagctcgcgc
      961 cggagctcga caaggagctc gagggcacgc gcagtgggga catcgtccgg gcgaacgcga
     1021 ggctgcccga gggcctcggt gagcacggcg gcaaggagat cgcgttccag gcactcgtca
     1081 aggaggtcaa gtcgaagcgc ctgccggcgg tcgacgatga cttcgcgaag acggcgtcgg
     1141 agttcgacac gctcgacgag ctccgcgccg acgtccgcga gaaggtcgcc gcactcaagc
     1201 acgccgccgc cgacggcgcc gtgcgcgacg aagcgctccg cgccctcgcc gggaaggtcg
     1261 acgtcgagct tcccgagcgg ctcgtcgacg gagagaccga agctcgcgtc gagcgcgcgc
     1321 gacagcgcgc cgagcagcag ggactcaagc tcgagcaggt gctcgacgcg cagcgcatcg
     1381 acgagctgca gttccgctcc gacgcccgcg ctcacgcgac gcgcgcgatc caggcagacc
     1441 tcgcgctcga ggccgtcgct cgagccgagg acctccgcgc gaccgacgag gacatcgacc
     1501 gtgccatcga ggtgatcgcg cgagacgtcg ggcggtcggc gaacgacgta cgccggacgc
     1561 tcgagcagac cggacagatc acgtctctgg ccggtgatat catccgagac aaagccctcg
     1621 acgtcgtggt cgagcatgcg aacgtcgtcg aggaaggggg gaagggaagc gggtcccccg
     1681 cttcggagga agggaaacca tgaagaacta cctcgttccc atcgtcatcg aacagaccaa
     1741 ccgcggcgag cgttcgttcg acatctactc gcggctcctg aaggagcgga tcgtgttcct
     1801 cggaaccccg atcgaggacc aggtcgcgaa cctcgtgatg gcgcagctct tgcacctcga
     1861 gtccgaggac cccgagaagg acatcaacat ctacatcaac tcgccgggcg gcgacatcac
     1921 gtcgctgttc gcgatctacg acacgatgca gtacatcaag cccgacgtgt cgacgatcgt
     1981 gatgggacag gccgcctcgg cggccgcggt gcttctcgcc gcgggaacga agggcaagcg
     2041 ctacgcgctc ccgcactcgc gcgtgctgat ccaccagcca cacggccagg ccggcggtca
     2101 ggccgtcgac atcgagatcc aggcgaagga gatccttcgg taccgctcgc tcctcgacga
     2161 gctgttggcc gagcacacga gccagccgct cgagaaggtg aagcacgaca ccgaccggga
     2221 cttcatcatg acgtcggagc aggcgaagga gtacgggatc atcgacgagg gcatctcgtc
     2281 gcggaagctg cgcgttgagt tgacggctgc gggggacggc aagggctaga ctgaccgggt
     2341 ccatacgagc gagcggacgg gggagcggtg gccaagttcg gggattcgga tcttctcaag
     2401 tgctccttct gcggcaagag ccagaagcag gtcaagaagc tcatcgccgg cccgggtgtg
     2461 tacatctgcg acgagtgcat cgacctctgc aacgagatca tcgaggagga gctctccgag
     2521 acaaccgagc tcaagctcga ggagcttccg aagccgcgtg agatctacaa gttcctgaac
     2581 gactacgtca tcggtcagga gcaggcgaag aaggtcctct cggtcgcggt ctaca
//
LOCUS       DASZOB010000258         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_174360, whole genome shotgun sequence.
ACCESSION   DASZOB010000258 DASZOB010000000
VERSION     DASZOB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174360"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VG709_04200"
     CDS             complement(<1..495)
                     /locus_tag="VG709_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32309.1"
                     /translation="MTDLAVRSRRATAELPAGRWRALALLSATLVFSMATWFSASAVI
                     PQLREVWTLSDTTAAWLTIAVQIGFVAGALVSSVFNLSDIVSPRHVILGGSVGAAAVN
                     LGLVAVDGPALGIPLRFATGFFLAGVYPPALKLMATWFRKGRGVALGVLVGALVVGHG
                     MPHLV"
     gene            complement(501..1508)
                     /gene="glpX"
                     /locus_tag="VG709_04205"
     CDS             complement(501..1508)
                     /gene="glpX"
                     /locus_tag="VG709_04205"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWC32310.1"
                     /translation="MSESKPDRNLAMELVRVTEAAAMAAGRWVGRGDKEAADQAAVDA
                     MRLMIDSVDMDGIVVIGEGEKDEAPMLYNGEQVGNGDGPPVDVAVDPIDGTTLTSKGM
                     GNAVSVIAVAERGSMFDPGGAVYMDKIAVGPEAAEAIDIDAGVGENVRRVAKAKGVDP
                     EDISVVVLERDRHKDLVAEIREAGARVMFITDGDVQGAIVAAAPERHAADMLMGVGGT
                     PEGVVGAAALKCIGGAMQGKLYPRNDDEKQKLVDGGYDLDRVLTTDDLVGGEEIFFAA
                     TGVTNGALLRGVAYRKDRVVTQSMVMRSRSGTVRYVEGHHQLDKLMRFSNIDYRSPRR
                     G"
     gene            complement(1505..>2079)
                     /locus_tag="VG709_04210"
     CDS             complement(1505..>2079)
                     /locus_tag="VG709_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWC32311.1"
                     /translation="PEVAEGQPCVAVLASEHAEALRLAAERFERDAYRVETTDDCTGL
                     EFCGTAKNVGAIGSGIVEGLGEQRDQEYRNARSVVFARATVEMADLVVAVGGRRETAL
                     GLAGVGDLLVTSLGGRNRQYGRAIGEGATPTHALAAMEGRGMTVEGVDSARDVHELAR
                     RGGLDLPVHEAVYRVVHESAEPSSILEVVR"
BASE COUNT          286 a          809 c          682 g          302 t
ORIGIN      
        1 cacgaggtgc ggcatgccgt gaccgacgac gagcgcgccg accagcacgc cgagcgcgac
       61 gccgcgaccc ttccggaacc acgtcgccat cagcttcagc gccggcgggt acacgcccgc
      121 gaggaagaag ccggtcgcga accgcagcgg gatcccgagc gccggaccgt cgacggcgac
      181 gagccccagg ttgactgccg ccgcgcccac ggacccgccg aggatcacgt ggcgcggcga
      241 cacgatgtcg gagaggttga acacgctcga gaccagcgct cccgcgacga acccgatctg
      301 gaccgcgatc gtgagccacg ccgccgtcgt gtcggacaac gtccatacct cgcggagctg
      361 agggatcacc gccgacgcgg agaaccacgt cgccatcgag aagacgagcg tcgccgacag
      421 gagcgccagc gcgcgccatc gtccggccgg gagctccgcc gtcgcccggc gcgaccggac
      481 ggcgaggtcc gtcaccccgc tcagccgcgg cgcggcgacc ggtagtcgat gttcgagaac
      541 cgcatcagct tgtcgagctg gtggtgccct tcgacgtagc ggaccgttcc ggagcgcgag
      601 cgcatcacca tcgactgggt cacgacccgg tccttccggt acgcgacgcc gcgcagcagt
      661 gcgccgttcg tgacgccggt cgccgcgaag aagatctcct cgccgccgac gaggtcgtcg
      721 gtcgtcagca cgcggtcgag gtcgtagccc ccgtcgacga gcttctgctt ctcgtcgtcg
      781 ttgcgcggat acagcttgcc ctgcatcgcg ccgccgatgc acttcagcgc cgctgcgccg
      841 accacgccct ccggcgtgcc gccgaccccc atgagcatgt ccgcggcgtg gcgctcgggc
      901 gccgccgcga cgatcgcacc ctggacatcg ccgtcggtga tgaacatcac gcgcgcgccc
      961 gcctcgcgga tctctgcgac gaggtccttg tgccggtcgc gttcgaggac gacgaccgag
     1021 atgtcctcgg gatcgacgcc cttcgccttc gcgacgcgtc ggacgttctc tccgacgccg
     1081 gcgtcgatgt cgatggcttc ggcggcctcc ggcccgaccg cgatcttgtc catgtacacc
     1141 gcgccgcctg ggtcgaacat cgagcctcgc tcggcgaccg cgatgaccga cacggcgttc
     1201 cccatcccct tcgacgtgag cgtcgtgccg tcgatcggat cgaccgcgac gtcgacgggc
     1261 gggccgtcgc cgttgccgac ctgttcgccg ttgtagagca tcggtgcctc gtccttctcg
     1321 ccctcgccga tcacgacgat gccgtccatg tcgacggagt cgatcatgag gcgcatcgcg
     1381 tcgacggccg cctggtcggc cgcctccttg tcgccgcgcc cgacccagcg ccccgccgcc
     1441 atcgcggccg cttccgtgac gcggacgagc tccatcgcga ggttccggtc cggcttcgac
     1501 tcgctcatcg cacgacctcc aggatgctcg acggctcggc gctctcgtgc acgactcgat
     1561 agacggcctc gtggacgggg aggtcgagcc cgcctcgccg cgcgagctcg tggacgtcgc
     1621 gggcggagtc cacgccctcc accgtcatcc cgcgaccctc catggccgcg agcgcgtgcg
     1681 tcggtgtcgc tccttccccg atcgccctgc cgtactggcg gttgcggccc ccgaggctcg
     1741 tgacgaggag gtcgccgacg cccgcgagcc cgagcgccgt ctcgcgccgg ccgccgaccg
     1801 ccacgacgag gtcggccatc tccacggtcg cgcgcgcaaa gacgacggag cgcgcgttcc
     1861 ggtactcctg gtcgcgctgt tccccgagcc cctcgacgat ccccgatccg atcgccccga
     1921 cgttcttcgc ggtcccgcag aactcgagcc ccgtgcagtc gtcggtcgtc tcgacgcgat
     1981 aggcgtctcg ctcgaaccgc tcggcggcga ggcggagcgc ctccgcgtgc tccgacgcca
     2041 acaccgcgac gcacggctgc ccctcggcga cctccggcg
//
LOCUS       DASZOB010000259         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_115796, whole genome shotgun sequence.
ACCESSION   DASZOB010000259 DASZOB010000000
VERSION     DASZOB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115796"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VG709_04215"
     CDS             <1..742
                     /locus_tag="VG709_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWC32312.1"
                     /translation="HCEEPRLSRGGQMHEGAASAALGFGGYPSVAETVMVARDLAIAR
                     YEGRPVHLLHLSARESVDELRRAQADGVAATGEVTPHHLCLTDEAVAALDPNLKMNPP
                     LRADEDREALVEGLRDGTIAAVASDHAPHARHEKEVPFEAAPFGVTGLETAFAALYTR
                     LVEPGLLSLGTLIERMSAGPAEVYGLERPRIATGADANLVLLALEAVWRVEETGFRSK
                     SANSWLLGETLRGRVLMTVAAGRLAYAA"
     gene            739..>1697
                     /gene="carA"
                     /locus_tag="VG709_04220"
     CDS             739..>1697
                     /gene="carA"
                     /locus_tag="VG709_04220"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWC32313.1"
                     /translation="MTAGHVSAEATGLLVLEDGTVFRGRSVGAEGMAFGEAVFTTAMS
                     GYQEVVTDPSYAEQLVCFTAPMVGNYGVAPMRSESDHAHACAVVMRRAGGREWTRWLA
                     SRGVVALEDVDTRGLVLHLRERGSLRAAAVSAAASVERVLERVRAQPPMAGRALVAGV
                     STREPYARGRGRLRVTVLDYGCKRSIVQRLLAAGAAVRVCPHWTPARAVLADAPDGVV
                     LSNGPGDPGALPGETAVVADLVGRVPLLGICLGHQLLARAVGYRTFKLRFGHRGANHP
                     VLDVRADCGLVTAQNHGFAVEGSGPEVTHVSLYDGTVEGLDVRG"
BASE COUNT          197 a          629 c          642 g          229 t
ORIGIN      
        1 ccattgcgag gagccccgcc tctcccgggg cggacagatg cacgagggcg cggcgtcggc
       61 ggcgctcggc ttcggcggct acccgtcggt cgccgagacg gtcatggtgg cgcgcgacct
      121 cgcgatcgcc cgttacgagg gccggccggt ccatctcctg cacctgtcgg cgcgcgagtc
      181 cgtcgacgag ctgcgacgcg cgcaggcgga cggcgtcgcc gcgaccggcg aggtgacgcc
      241 ccaccatctc tgcctgacgg acgaggcggt ggccgcgctt gacccgaacc tcaagatgaa
      301 cccgccgctt cgcgccgacg aggaccgcga ggcgctcgtc gagggcctgc gcgacgggac
      361 gatcgctgcg gtcgcgagcg accacgcccc gcacgcccgc cacgagaagg aggtcccctt
      421 cgaggcggcg cccttcgggg tgacggggct cgagacggcg ttcgcggcgc tctacacgcg
      481 ccttgtcgag ccgggcctgc tctcgctcgg gacgctgatc gagcgcatga gtgcggggcc
      541 cgccgaggtc tacggcctcg agcgaccgcg gatcgcgacg ggcgccgacg cgaacctcgt
      601 ccttctcgcc ctcgaggccg tgtggcgcgt cgaggagacg ggcttccggt cgaagtccgc
      661 gaactcctgg ctgctcggcg agacgctgcg cgggcgcgtg ctcatgacgg tcgcggccgg
      721 gcggctggcg tacgcggcat gaccgccggg cacgtgagcg ccgaggcgac cggcctcctc
      781 gtcctcgagg acgggaccgt cttccgaggc cgctcggtgg gcgcggaggg gatggcgttc
      841 ggcgaggccg tcttcacgac cgcgatgtcg ggctaccagg aggtggtcac cgacccgagc
      901 tacgccgagc agctcgtctg cttcacggcg cccatggtcg gcaactacgg cgtcgcgccg
      961 atgcgctcgg aatccgacca cgcccatgct tgcgccgtcg tcatgcgccg cgccggcgga
     1021 cgtgagtgga cgcgctggct cgcatcgcgc ggcgtcgtcg ccctcgagga cgtcgacacg
     1081 cgcggcctcg tgctccacct gcgcgagcgg gggtcgctgc gcgcggcggc cgtctcggcg
     1141 gcggcgtccg tcgagcgcgt gctcgagcga gtacgcgcgc aaccgcccat ggcggggcgc
     1201 gccctcgtcg ccggcgtgtc caccagggag ccgtacgcgc gcgggcgggg gaggctgcgc
     1261 gtgaccgtcc tcgactacgg ctgcaagcgg tcgatcgtcc agcgtctcct cgcggcgggc
     1321 gcggcggttc gagtctgtcc gcactggacg cccgcgcgcg cggtgctcgc ggacgcgccg
     1381 gacggcgtcg tcctctcgaa cggcccgggg gatcccggcg cgctgccggg cgagaccgcg
     1441 gtcgtcgccg acctcgtcgg ccgcgtgccg ctcctcggga tctgcctcgg ccaccagctc
     1501 ctcgcgcggg cggtcggcta ccggacgttc aagctccgct tcggccaccg gggcgccaat
     1561 cacccggtcc tcgacgtgcg cgccgactgc ggcctcgtca cggcgcagaa ccacggcttc
     1621 gccgtcgagg gaagcgggcc cgaggtgacg cacgtgtccc tctacgacgg caccgtcgag
     1681 ggcctcgacg tccgggg
//
LOCUS       DASZOB010000260         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_156880, whole genome shotgun sequence.
ACCESSION   DASZOB010000260 DASZOB010000000
VERSION     DASZOB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156880"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VG709_04225"
     CDS             <1..598
                     /locus_tag="VG709_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32314.1"
                     /translation="DGADGAATDMAKLFGDAEIEEALPGELETTLRVVVGQTFQGTLA
                     PAPKDATPKRRPPSVVRDPQSAAPLVKEAQRKVDFPLLVPTVRETRSTLADVEPMRVY
                     RLNGDGAVRLVYNGPSGLDYWGIQQTSWTDAPILDGPTVTRRMGGREYRLYFNGARLH
                     MVAFEENDAVYWVSNTLLDVLSNETMLAIAKGLRPVDG"
     gene            654..2000
                     /locus_tag="VG709_04230"
     CDS             654..2000
                     /locus_tag="VG709_04230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWC32315.1"
                     /translation="MDVRQPIGVIGAGWVGLVTAACFADMGHPVTVRDIVPERVADLE
                     AGRMPIHEPGLSGLLDRNRERVRFTLAIEDVFREARLAFVCVGTPSTYSGDSDLSAVR
                     RVLDELPDFDERAVIVMKSTVPVGTGEKIRHELDSRGLAHVGYVSNPEFLAEGTAIEN
                     FRHPDRVVVGSFAESDGDAVASLYEPLGAPVLRTDIASAEMIKLASNAFLVTRISFIN
                     EIANVCELVGADVARVAEGVGLDHRLGPHFLRAGIGYGGSCFPKDSLALKQLAANSGY
                     HFQLLSAVIEVNELQKRRVVAKLAKHLGSLRGKTVALLGLAFKPNTDDLRDAPSLVLA
                     SRLLAEGARVRAWDPVALDEAGRLLSGVTFAETTVDAVTDADAAVVVTEWEELRGLAS
                     AEVRDAMRNPLVIDGRNMLDPEIVRAAGFAYEGIGRAASSLRAVPETEEREPGLRR"
     gene            2006..>2322
                     /locus_tag="VG709_04235"
     CDS             2006..>2322
                     /locus_tag="VG709_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWC32316.1"
                     /translation="MEAILLAGGKGERLGEAARGRPKPLVPVAGRPLAAYQVARLAAA
                     GVDRVIVSCAAGREPLFEAELAGLGVDVVPVGEPEPLGRGGGLRFAAAARVEDGPVYA
                     LNG"
BASE COUNT          315 a          838 c          845 g          324 t
ORIGIN      
        1 ggacggcgcc gacggcgccg ccacggacat ggcgaagctc ttcggcgacg ccgagatcga
       61 ggaggctctg cccggcgagc tcgagacgac gctccgggtc gtcgtcgggc agacgttcca
      121 gggaacgctc gctccggctc cgaaggacgc gactccgaag cgccggccac ccagcgtggt
      181 gcgggaccct cagtcggccg ctccgctggt gaaggaggcg cagcgaaagg tggacttccc
      241 gctcctcgtg ccgacggtcc gcgagacccg ctccaccctc gcggatgtcg agccgatgcg
      301 ggtctaccga ctgaacggag acggcgcggt tcgactcgtc tacaacggcc cctcgggcct
      361 cgactactgg ggaatccagc agacgagctg gacggacgcg ccgattctcg atggtccgac
      421 ggtgacgcgc cggatgggag ggcgcgagta tcgcctctac ttcaacggcg cgcgcctcca
      481 catggtcgcc ttcgaggaga acgacgcggt gtactgggtc tcgaacacgc tgctcgacgt
      541 cctctcgaac gagacgatgc tcgcgatcgc caagggcctc cggcccgtcg acggctaggg
      601 ccgtggcgcc gtccgcgggc ggggcaggcc gcgcccctac actctcctcg ggcatggacg
      661 tgcgacagcc catcggcgtc atcggggccg gctgggtcgg gctcgtgacg gccgcctgct
      721 tcgcggacat ggggcacccc gtcacggtgc gcgacatcgt gcccgagcgc gtcgccgacc
      781 tcgaggcggg gcgcatgccg atccacgagc ccggcctgtc cggcctcctc gaccggaacc
      841 gggagcgcgt gcgcttcacg ctcgcgatcg aggacgtctt ccgtgaggcg cggctcgcgt
      901 tcgtgtgcgt cggcacgccg tccacctact cgggcgactc ggacctctcg gccgtccggc
      961 gcgtcctcga cgagctgccg gacttcgacg agcgggcggt catcgtgatg aagagcacgg
     1021 tcccggtcgg gaccggcgag aagatccggc acgagctcga ttcgcgcggg ctcgcgcacg
     1081 tcggctacgt ctccaacccc gagttcctcg cggaggggac cgcgatcgag aacttccgcc
     1141 acccggaccg cgtcgtggtc gggagcttcg cggagtcgga cggcgacgcc gtcgcgtcgc
     1201 tctacgagcc cctcggcgcg ccggtcctcc gcaccgacat cgcgtcggcc gagatgatca
     1261 agctcgcctc gaacgcgttc ctcgtgacgc gcatcagctt catcaacgag atcgcgaacg
     1321 tgtgcgagct cgtgggcgcg gacgtcgcgc gcgttgccga gggcgtcggt ctcgaccatc
     1381 gcctcggacc gcacttcctc cgggcgggga tcgggtacgg cggcagctgc ttcccgaagg
     1441 attcgctcgc gctcaagcag ctggccgcca actcgggcta ccacttccag ctcctctcgg
     1501 ccgtcatcga ggtgaacgag ctgcagaagc ggcgagtcgt cgccaagctc gcgaagcacc
     1561 tcggctccct ccgggggaag accgtcgcgc ttctcgggct cgcgttcaag ccgaacaccg
     1621 acgacctgcg cgacgcgccg agcctcgtcc tcgcctcgcg cctcctcgcg gagggcgccc
     1681 gcgtgcgggc gtgggacccc gtcgcgctcg acgaggcggg gcgccttctc agcggcgtca
     1741 cgttcgccga gacgaccgtg gacgccgtca cggacgccga cgcggccgtc gtcgtcaccg
     1801 agtgggagga gctgcgcggg ctcgcatccg ccgaggtgcg ggacgcgatg cgaaacccgc
     1861 tcgtgatcga cggacgcaac atgctcgacc ccgagatcgt ccgtgcggcc gggttcgcct
     1921 acgaggggat cggccgggcc gcctcctcgc tccgcgcggt gccggagacg gaagagcgcg
     1981 aaccaggcct tcgccggtag gccgcgtgga ggcgatcctc ctcgcgggcg gaaagggcga
     2041 gcggctcggc gaggcggcgc gcgggcgtcc gaagccgctc gtgccggtcg ccgggcggcc
     2101 gctcgcggca tatcaggtgg cccggctcgc cgccgcgggc gtcgaccgcg tgatcgtgag
     2161 ctgcgcggcc gggcgagagc cgctcttcga ggcggagctc gccggcctcg gagtcgacgt
     2221 cgtcccggtg ggcgagcccg agccgctcgg gcgcggcggc ggcctccgct tcgccgccgc
     2281 ggcgcgggtc gaggacggcc ccgtgtacgc gctgaacggc ga
//
LOCUS       DASZOB010000261         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_56100, whole genome shotgun sequence.
ACCESSION   DASZOB010000261 DASZOB010000000
VERSION     DASZOB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56100"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VG709_04240"
     CDS             complement(<1..318)
                     /locus_tag="VG709_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32317.1"
                     /translation="MSTPGDATDAKPAKRDALAEEARRLREQAAAEKAAGNDWRAARL
                     LDRALAIYRDSGDLRGVTQVMGELAPVGGVKGTSWPVALGFLVVLLAVAAGVIFLGIR
                     LGGG"
     gene            complement(325..1368)
                     /locus_tag="VG709_04245"
     CDS             complement(325..1368)
                     /locus_tag="VG709_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWC32318.1"
                     /translation="MEYRVLGRTGVRVSELCLGAMMFGSLGNRDRRDCVRMIQRALDG
                     GINMIDTADIYSGGESEEIVGEALRGRRDEVVLATKLNRRMGPGLNDGGNSRLWIMRA
                     VEASLRRLGTDHIDLYQLHRPDPRTDLEESLEALSDLVHQGKVRYVGTSAFPAFELVE
                     AQWIAERRGLSRPACEQVSYSMMVRHAERETFPVAQRYRLGAIVYSPLAGGWLTGRYR
                     RGEPPPEGTRMDRAVKEGLFGRWFSPERPETERRFDVVERLSLVAQKAGASLTEMAIA
                     FTLAHPAVSASIIGPRTMEQLEGALEAVEVRLDDDTLDAIDDAVAPGTLVDDADRGWN
                     PPWMAPEARRRRD"
     gene            complement(1386..1961)
                     /locus_tag="VG709_04250"
     CDS             complement(1386..1961)
                     /locus_tag="VG709_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWC32319.1"
                     /translation="MPKLWSETIEAHRREVRDAIVDTAAALVAHHGLRSVTMSQIADE
                     TGIGRATLYKYFPDVEAILHAWHERQIAGHLRYLAEVRDGAPEGRRLEAVLHAYADLS
                     RGSHGHRDRDLAAFLHRDDQMIRAERELVRMIRDVIADDAAAGVLRGDVPPDELATYC
                     LHALAAARAVSSKAAVRRLVDVTIDALRRAG"
     gene            2186..2689
                     /locus_tag="VG709_04255"
     CDS             2186..2689
                     /locus_tag="VG709_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32320.1"
                     /translation="MGSGLRKLALTVHLTVSVGWIGAAVAYLALGISAATTESPPTIR
                     AAWTAMEVTGRYVIVPLAVASVLTGLVMALGTRWGLFRHYWVLISFTLAVVAAVVVLL
                     HMPTVSSVADAVRGSADPELAGFGGDLLHPALGLVVLLVVQVLNLYKPAGMTRYGRRK
                     QRSAAAR"
     gene            complement(2697..3839)
                     /locus_tag="VG709_04260"
     CDS             complement(2697..3839)
                     /locus_tag="VG709_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWC32321.1"
                     /translation="MAAEGKTQWRDLHDEVVATGLCTGCSACVVVCPFHVLGYENDKP
                     WQMEEGPPDQCTHGDRGCDICTRACPRFREWEPDIERLFFGRTRLPEEVIGINRDIVL
                     SRATDPRALAQGQDGGVVSALLIWGLETGQIDGALTSKLSDARPWDAEPAVVTTPEDV
                     LATAGSRYTYSANPLALPKAAEMGLKQVALVGMSCQASVTGVLEARRVNKWRRRIAWT
                     LGLLCSKTFTYEGLMEEIAQEELGLGLDDIVRVNVKGKLLFYTRDGEEHQYSLKKAHR
                     FTRPGCMLCPDFAAEHADISFGGLGQSAGWTLTVIRTELGEDIWRRALEAGIVEDRPA
                     SEDPAAVALMEKLATKQRQRWPVDDGNGAARPGVVPEPVEGTGIRD"
BASE COUNT          544 a         1407 c         1446 g          635 t
ORIGIN      
        1 cccccccccg aggcggatcc cgaggaagat cacgcctgcc gcgacggcca gcagcacgac
       61 gaggaagccg agggcgacgg gccacgacgt ccctttcacg ccgccgaccg gggcgagctc
      121 gcccatcacc tgcgtgacgc cgcgcaggtc gccgctgtcc cggtagatcg cgagcgctcg
      181 gtcgaggagg cgtgccgccc tccagtcgtt cccggcggcc ttctccgcgg cggcctgctc
      241 gcgaagccgc cgggcctcct cggcgagcgc gtcgcgcttc gcgggcttgg cgtcggtcgc
      301 gtcgccgggc gtcgacatgg gcggctagtc tcgcctccgg cgcgcttcgg gcgccatcca
      361 gggcgggttc cagccgcggt cggcgtcgtc gaccagcgtg cccggggcga cggcatcgtc
      421 gatcgcgtcg agcgtgtcgt cgtcgagccg gacttcgacg gcttcgagcg ctccctccag
      481 ctgctccatc gtccgcggcc cgatgatcga tgccgacacc gcagggtgcg cgagcgtgaa
      541 cgcgatcgcc atctcggtca gcgacgcccc ggccttctgc gcgaccagcg acaggcgctc
      601 gacgacgtcg aaccgacgct ccgtctcggg gcgctccgga gagaaccagc gtccgaacaa
      661 cccctctttc acggcacggt ccatccgtgt cccctcgggc ggcggctcgc cgcggcggta
      721 tcgtccggtc agccagccgc cggcgagcgg gctgtacacg atcgcgccga ggcggtatcg
      781 ctgcgctacc gggaacgtct cgcgctcggc gtggcggacc atcatcgagt acgacacctg
      841 ctcgcaggcc ggtcgggaga gcccgcgccg ctcggcgatc cactgggcct cgacgagttc
      901 gaacgccggg aaggccgacg tgccgacgta gcggaccttc ccctggtgga cgaggtcgct
      961 gagcgcctcg aggctctcct cgaggtcggt gcgcgggtcg ggccggtgga gctggtagag
     1021 gtcgatgtgg tcggtcccga gccgccgcag gctcgcctcg acggcccgca tgatccagag
     1081 ccgcgagttg ccgccgtcgt tgaggccggg tcccattcga cggttgagct tcgtcgcgag
     1141 cacgacctca tcgcgccggc cgcggagggc ctcgccgacg atctcctcgg actcgccacc
     1201 cgagtagatg tcggcggtat cgatcatgtt gatcccgccg tcgagcgcac gctggatcat
     1261 ccgcacgcag tcgcggcggt cgcggttccc gagcgagccg aacatcatcg cgccgaggca
     1321 cagctcgctc acgcggacgc cggtacgccc gagcacccgg tactccatgg cgcgcgattc
     1381 tatggtcagc cggcgcgtcg gagcgcgtcg atggtgacgt cgacgagccg gcggaccgcc
     1441 gccttcgacg agaccgcgcg agccgcggcg agggcatgga ggcagtacgt cgcgagctcg
     1501 tctggaggga cgtcgccgcg aagtaccccc gcggcggcgt cgtcggcgat cacgtctcgg
     1561 atcatgcgga cgagctcgcg ttcggctcgg atcatctgat cgtcgcgatg gaggaacgcg
     1621 gcgaggtcgc ggtcgcggtg accgtgcgac ccgcgtgata agtcggcgta ggcgtggagc
     1681 accgcctcga gtcggcggcc ctccggtgcc ccgtcacgga cctcggcgag ataccggagg
     1741 tggccggcga tctggcgctc gtgccacgcg tgcaggatcg cttcgacgtc cgggaagtac
     1801 ttgtagagcg tcgctcgacc gatcccagtc tcgtcggcga tctgcgacat cgtgacggag
     1861 cggagcccgt ggtgcgctac gagcgccgcc gcggtatcga cgatcgcgtc ccgaacctca
     1921 cggcggtggg cctcgatcgt ctcgctccac agcttcggca cggcccgagc ataccgcact
     1981 gcgatacacc gtgacttgac atagacatcg tgtcttgaaa aagtggcgaa ccatgccagg
     2041 gtgggtgaag accctgatct tggtcgcggt gctcgccggc gtcgccgcgt tcgtcatctc
     2101 ctcgctcgcc ggggtcgagc acggacccgg acgacacgtg ccgcccgtcc agcacgggcc
     2161 atagggaagg ccgcgtccga tgctcatggg atccggtctg cggaagctgg cgctcacggt
     2221 gcatctcacg gtctccgtgg gctggatcgg cgcggccgtc gcgtacctgg cgctgggcat
     2281 ctcggcggca acgaccgagt ctcctccgac gatccgtgcg gcgtggacgg cgatggaggt
     2341 gacgggccgg tacgtgatcg tgccgctcgc ggtcgcatcg gtcctcaccg gcctggtgat
     2401 ggcgctcgga acgagatggg gcctgttccg ccactactgg gtgctgatct cgttcacgct
     2461 ggcggtcgtc gcggcggttg tcgttctcct ccacatgccg accgtcagct cggtcgccga
     2521 tgcggtgcga ggttcggcag accccgagct cgcggggttc ggaggcgacc tgctgcatcc
     2581 ggcgctggga ctcgtcgttc tgctggtggt ccaggtactg aacctctaca agccggcggg
     2641 gatgacgcgg tacgggcggc ggaagcagcg gagtgcggcc gctcgctagc gggctgtcag
     2701 tcgcggatgc cggtgccctc gacgggctcc gggacgacgc cggggcgtgc ggcgccgttg
     2761 ccgtcgtcga ccggccagcg ctgccgctgc ttcgtcgcga gcttctccat cagcgcgacg
     2821 gccgcggggt cctcgctcgc ggggcggtcc tcgacgatcc cggcctcgag cgcgcgacgc
     2881 cagatgtcct cgccgagctc cgttcggatc acggtgagcg tccagccggc gctctgcccg
     2941 aggcctccga acgagatgtc cgcgtgctcg gccgcgaagt cggggcacag catgcagccg
     3001 ggccgcgtga accggtgcgc cttcttgagc gagtactggt gctcctcgcc gtcgcgcgta
     3061 tagaagagga gcttcccctt cacgttgacc cggacgatgt cgtcgagccc gaggccgagc
     3121 tcctcctgcg cgatctcctc catgagtccc tcgtacgtga acgtcttcga gcacaggagc
     3181 ccgagcgtcc acgcgatccg gcggcgccac ttgttgacgc gtcgcgcctc gagcacgccg
     3241 gtcacgctcg cctggcacga catcccgacg agcgcaactt gcttcagccc catctcggcg
     3301 gccttcggga gggcgagggg gttcgccgag tacgtgtagc gggaccctgc ggtcgcgagc
     3361 acgtcctccg gagtcgtcac gaccgccggc tcggcgtccc aggggcgcgc atcggagagc
     3421 ttcgaggtga gcgcgccgtc gatctggccg gtctccagcc cccagatgag gagcgcggac
     3481 acgacgccgc cgtcctgccc ttgcgcgagc gcgcgagggt cggtcgcccg cgacagcacg
     3541 atgtcgcgat tgatgccgat gacctcctcg ggaagccgcg tgcgtccgaa gaagaggcgc
     3601 tcgatgtcgg gctcccattc tcggaagcgc gggcacgccc gcgtgcagat gtcgcagcct
     3661 cggtcgccgt gggtgcactg atccggcggg ccctcctcca tctgccacgg cttgtcgttc
     3721 tcgtagccga ggacgtggaa gggacagacg acgacgcagg cggagcagcc ggtgcagagc
     3781 ccggtcgcga ccacctcgtc gtgcaggtcg cgccactggg tcttgccctc ggccgccacg
     3841 aggcgagtat acgtccggcc cttccgggcg aagcggtcgc ggcatgattg cgcgcttccc
     3901 aaggtatcgt cgtgcaatgc aagcgatgga gggccggatg aggggtcgca gggtcgcgac
     3961 ggccgccgtt gccgcggcgc tcgtcctcgt cgccgtcggc tgcggcggga acggcggcga
     4021 cggcgggaac gg
//
LOCUS       DASZOB010000262         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_5933,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000262 DASZOB010000000
VERSION     DASZOB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5933"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..932)
                     /locus_tag="VG709_04265"
     CDS             complement(144..932)
                     /locus_tag="VG709_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32322.1"
                     /translation="MVLAQVDLRGGLEAAWERIVLFGPKLLGFLAILLVGYFVAKAIQ
                     KILDKVLERVGFNRWVERGGIKRALERSKYDASDILSKIVFYIIFLCVLQLAFGVFGP
                     NPISELLSGVVAFLPKLFAAILILVVAGAIAAAVRELVATALGGLSYGNLLANVAMAA
                     IWFVGFSAALNQIEIAPAIVNGLFYAVLALVVGSAIVAIGGGGIEPMRERWRRALERL
                     DDEAPKVRDEAQGAGDRIRDRAEEVREQAHSDTATDRETGSDTR"
     gene            complement(1041..1184)
                     /locus_tag="VG709_04270"
     CDS             complement(1041..1184)
                     /locus_tag="VG709_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32323.1"
                     /translation="MRKFFLFSLLIGAAVAIYRKLKADRTTSTYDLDAATDYPTTTGT
                     PTL"
     gene            1375..>1840
                     /locus_tag="VG709_04275"
     CDS             1375..>1840
                     /locus_tag="VG709_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWC32324.1"
                     /translation="MVEPAMEPDALEVLTVDHRTVENLFALYDSTPAQDVEARDKVVR
                     EIVRELSIHAAVEEQLLYPLMRDRLEDGERLVREALDEHQEVKESLAAIGKADPATGD
                     RHAGVTALIGDVRHHVEEEEGELFPKLRAAAAPEELRDLAGRIESAKRMAPTR"
BASE COUNT          314 a          639 c          622 g          265 t
ORIGIN      
        1 gatggaggat ctctcccgcg cgcggcacgc gatcgacgcg cgcgaactgc acccactcga
       61 cgtggccgac cacggcgacg cgcacgacgg gagtctgcca tcccgcgccc ctcgtctccg
      121 agaggtcctg acgcgctgat ccgctagcgc gtgtcggagc ccgtctctcg gtcggtcgcg
      181 gtatcggagt gagcctgctc gcgcacctct tcggcgcggt cgcggatacg gtcgccggcg
      241 ccctgcgcct cgtcccgcac ctttggggcc tcgtcgtcga gacgctcgag agcccgccgc
      301 caccgctcgc gcatcggctc gatgccaccg ccgccgatcg cgacgatggc cgatccgacg
      361 accagagcga gcacggcgta gaagagcccg ttgacgatgg ccggggcgat ctcgatctgg
      421 ttcagcgcgg ctgagaaccc gacgaaccag atggcagcca tggcgacgtt cgccaagaga
      481 ttcccgtacg agaggccgcc gagagcagtt gcgacgagct ccctcaccgc tgccgcgatg
      541 gcgcccgcga ccacaaggat gaggatcgcg gcgaacagct tcggcaggaa cgcgaccacg
      601 ccggaaagca gctcgctgat ggggttcggc ccgaacaccc cgaaagcgag ctgcagcacg
      661 cacaggaaga tgatgtagaa gacgatcttg ctcaggatgt cgctcgcgtc gtacttcgag
      721 cgctcgagcg ctcgcttgat cccgccgcgc tcgacccacc ggttgaaacc gacccgctcg
      781 agcaccttgt cgaggatctt ctggatcgcc ttggcgacga agtatccgac gaggaggatc
      841 gccaaaaacc cgaggagctt cggcccgaag aggacgatcc gctcccacgc ggcctcgaga
      901 cctccgcgga ggtcaacctg cgcgagcacc atcgtcctca ccacctcctc ggtccagcgc
      961 ccatggcgcc ggtcgagacg ccgccgcagc cagctacgcg cgcggctcga acgtcgagaa
     1021 gggcgccgaa cgcccgtccg ctacagggtc ggcgttccgg tcgttgtggg gtagtcggtg
     1081 gccgcgtcga ggtcgtacgt cgacgtggtg cggtccgcct tcagcttgcg gtagatcgcg
     1141 accgcggccc cgatcaacag ggagaacagg aagaacttcc tcatgtgacc tccttcgagt
     1201 tcgtttgtgc ccaccggctc gcgatcggaa accagcgtgt ggatcgtccg gcttcctgcg
     1261 ccgctccgtc ggggggacgt gacgtcccgc gtatgagcgc atcttcgacc gtgtatccgg
     1321 gtaagtcggg cccaccgacc cacgaacacc aacccgacga acgaggaggc cgccgtggtg
     1381 gagcccgcga tggagccgga tgcgctcgag gtgttgaccg tcgatcaccg caccgtcgag
     1441 aacctgttcg cgctgtacga ctcgaccccg gcgcaggacg tggaggctcg ggacaaggtc
     1501 gtgcgggaga tcgtgcgcga gctctcgatc cacgccgccg tggaggagca gctgctgtac
     1561 ccgctgatgc gagaccggct cgaggacggc gagcggctcg tgcgcgaggc gctcgatgag
     1621 catcaggagg tgaaggagtc gctcgccgcc atcgggaagg ccgacccggc gaccggcgac
     1681 cgccatgcgg gcgtcaccgc gctcatcggc gacgtccgcc accacgtcga ggaggaggag
     1741 ggcgagctct tcccgaagct gcgcgccgcc gcggctcccg aagagctccg cgacctcgcc
     1801 gggcgcatcg agtcagcgaa gcgtatggcg ccgacgcgcc
//
LOCUS       DASZOB010000263         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_25862, whole genome shotgun sequence.
ACCESSION   DASZOB010000263 DASZOB010000000
VERSION     DASZOB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25862"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VG709_04280"
     CDS             <1..214
                     /locus_tag="VG709_04280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWC32325.1"
                     /translation="ARLTRYPPGLAGALRKIAAVPNQMRVANNATAHLWISQPSRLPG
                     FSYSFAERLFSTHPPLEERIKALEEM"
     gene            290..1333
                     /locus_tag="VG709_04285"
     CDS             290..1333
                     /locus_tag="VG709_04285"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWC32326.1"
                     /translation="MALSTRGKSIAGGLAAVVLLGGGFLGYRLLTKAPNEPILGDVLG
                     GDGLPDECPLLGVDPEGDVPNRPALAVKVENNPDSRPQAGLAEADIVYEEEAEGGITR
                     FMAIYQCTSSSRIGPIRSARLVDPNILDQYGEPLFAYAGAAPTVRNAIDEHASIVQVN
                     YIDAADAYTEDPNREIPHHLFSSTRALYRAARAAGRAHLPEAVFTYDEDVPTGRRAQE
                     IRANFNPNVADVIWRYDRASGRYLRYHGTVAHTLEDGGQISSTNVVVQIVERIQTDIT
                     DPAGNPVFDFEVTGSGRAFVFRDGRMIRGRWERPNDDGVTRFVARNGDEIPLAPGNTW
                     VELYPADAPPLEF"
     gene            1400..2290
                     /gene="pdxS"
                     /locus_tag="VG709_04290"
     CDS             1400..2290
                     /gene="pdxS"
                     /locus_tag="VG709_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWC32327.1"
                     /translation="MDDKQTGTFRVKSGLAEMLKGGVIMDVTTAEQAKIAEDAGAVAV
                     MALERVPADIRAEGGVARMADAAKVQEIIDAVSIPVMAKARIGHFVEAQILQALGVDY
                     VDESEVLTPADERHHINKWDFTVPFVCGARDLGEALRRTAEGAAMIRTKGEAGTGNVV
                     EAVRHVRTITGEISRLRGLRDEELFSAAKELGAPLELVRQVAAAGKLPVVNFAAGGLA
                     TPADAALKMQLGVEGVFVGSGIFKSADPASRARAIVEATTYWGDPEILAKVSRGLGEP
                     MRGIEIGELGEEGLLQTRGW"
     gene            2287..2853
                     /locus_tag="VG709_04295"
     CDS             2287..2853
                     /locus_tag="VG709_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32328.1"
                     /translation="MNAADARSHAGRRAAAVIPSLYGVVGAAVGLWLFIAAMLEVLVA
                     TARLALDLWFESTWADPLRSVTAAFPSAVVAAVVVWWHVRYGIRGGREGAREGSWRAS
                     LYFHLVALVALVAALIGAVEVKSAAADLVAPQCIAPATTDYVPGRSCFPPSSVAVRNA
                     IEGAALFLVASPIFLWHIARAPRRSSRG"
     gene            2886..>3148
                     /gene="pdxT"
                     /locus_tag="VG709_04300"
     CDS             2886..>3148
                     /gene="pdxT"
                     /locus_tag="VG709_04300"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWC32329.1"
                     /translation="MKAGVLALQGDFREHARVLEDLGATPVLVRTVDELGEVSCLAIP
                     GGESTTMSKLARRYGLVEPIRERASRGMPVFGTCAGMIVMAREV"
BASE COUNT          467 a         1090 c         1110 g          481 t
ORIGIN      
        1 cgcgcggctc acccgctacc cgccgggcct cgcgggagcg ctgcggaaga tcgccgcggt
       61 cccgaaccag atgcgcgtcg cgaacaacgc gaccgcgcat ctgtggatct cccagccgtc
      121 ccggctcccg ggcttctcgt actcgttcgc cgagcgtctg ttctcgactc atccgccact
      181 cgaggaacgg atcaaggccc tcgaagagat gtagttcgtt gaagaacgag gagacgccgg
      241 gtagcatcgg gcgccgttcc tccttccccc cgacctctgg aggtacttcg tggctctgtc
      301 gacgcgcggg aaatcgatcg cgggcggcct cgcggcagtc gtgctgctcg gcggcggctt
      361 cctcggctac cgcctcctga cgaaggcgcc gaacgaaccg atcctcgggg acgtcctcgg
      421 cggcgacggg ctccccgacg agtgtccgct cctgggcgtc gatcccgagg gcgacgtccc
      481 gaaccgcccc gcgctcgccg tgaaggtcga gaacaatccc gactcgaggc cgcaggcggg
      541 gctcgccgaa gccgacatcg tctacgagga ggaggcggag ggcggcatca cgcggttcat
      601 ggcgatctac cagtgcacga gctcctcgcg catcggtccg atcaggagcg cgcggctcgt
      661 cgaccccaac atcctcgacc agtacggcga gccgctgttc gcgtacgccg gcgcggcgcc
      721 gacggtccgg aacgcgatcg acgagcacgc gagcatcgtc caggtgaact acatcgacgc
      781 tgccgatgcg tacacggagg atccgaaccg cgagatcccc caccacctct tctcgtcgac
      841 tcgcgcgctc taccgcgccg ctcgtgcagc cggccgcgcg cacctccccg aggccgtctt
      901 cacgtacgac gaggacgtcc ccacggggag gcgggcacag gagatccgcg cgaacttcaa
      961 cccgaacgtc gcggacgtca tctggcggta cgaccgtgct tccggccgct acctccggta
     1021 ccacggaacc gtcgcgcaca cgctcgagga cgggggccag atcagctcga cgaacgtcgt
     1081 cgtccagatc gtggagcgga tccagacgga catcaccgat cccgcaggca accccgtttt
     1141 cgacttcgag gtcaccggga gcggaagggc gttcgtcttc cgggacggga ggatgatccg
     1201 aggtcgctgg gaacgcccga acgacgatgg cgtcacccgg ttcgtggcgc gcaacggcga
     1261 cgagatcccg ctcgcgccgg ggaacacgtg ggtggagctc tacccagccg acgccccgcc
     1321 gctcgagttc tgaggtccat cgtcacgcgc ctcgcccgcg ccgtatcctg agggcggcgc
     1381 gagttcgagg aggcgggcga tggatgacaa gcagacgggg acgttccggg tcaagtccgg
     1441 gctcgccgag atgctgaagg gcggcgtcat catggacgtg acgaccgccg agcaggcgaa
     1501 gatcgccgag gacgccggcg cggtcgcggt catggcgctc gaacgggtcc cggcggacat
     1561 ccgagccgag ggcggggtcg cgcgcatggc cgacgccgcc aaggtccagg agatcatcga
     1621 cgccgtgtcg atccccgtca tggccaaggc ccgcatcggc cacttcgtcg aggcgcagat
     1681 cctccaggcc ctcggtgtcg actacgtcga cgagagcgag gtcctgacgc cggccgacga
     1741 gcgccaccac atcaacaagt gggacttcac ggtccccttc gtgtgcggag cgcgcgatct
     1801 cggagaggcg ctgcgccgca ccgccgaagg cgccgcgatg atccggacga agggcgaggc
     1861 ggggaccggc aacgtcgtcg aggcggtccg ccacgtccga acgatcaccg gcgagatctc
     1921 gcgcctaagg ggtctccgcg acgaggagct cttttcggcc gcgaaggagc tcggcgcgcc
     1981 gcttgagctc gtccgtcagg tcgccgccgc ggggaagctg ccggtcgtga acttcgccgc
     2041 cggcggcctc gccacgccgg ccgacgcggc gctcaagatg cagctcgggg tcgaaggtgt
     2101 gttcgtcggc tcggggatct tcaagtccgc cgacccggcg tcccgggcgc gtgcgatcgt
     2161 cgaggcgaca acgtactggg gcgacccgga gattctcgcg aaggtctccc gcgggctcgg
     2221 cgagccgatg cgtgggatcg agatcggcga gctcggcgag gaggggctcc tccagacccg
     2281 cggctggtga acgctgcaga cgctcggtcg cacgccggac gtcgcgccgc tgcggtcatc
     2341 ccgtcgctgt atggagtggt cggtgcagcc gtcgggctct ggctcttcat cgctgcgatg
     2401 ctcgaggtgc tcgtcgcgac ggcacggctc gctctcgatc tctggttcga gtcgacatgg
     2461 gccgacccgc ttcggtccgt tacggcggcg ttcccttcgg ccgtggttgc ggccgtcgtc
     2521 gtctggtggc acgtgcggta cgggatccgc ggagggcgtg aaggtgctcg cgaagggtcg
     2581 tggcgcgcgt cgctctactt ccacctcgtc gcgctcgttg cgctcgtcgc ggccctcata
     2641 ggcgccgtcg aggtgaagtc ggccgccgcg gacctcgtcg cgccgcagtg catcgcgccg
     2701 gcgaccaccg actacgtgcc ggggcgctcg tgcttcccgc cctcgagcgt ggcggtccgg
     2761 aacgccatcg agggagccgc cttgttcctc gtcgcgtcgc cgatcttcct gtggcacatc
     2821 gcgcgagcac cgcgacgaag cagccgcggc tgagcgccgc ggttcgctgg ctatcatgag
     2881 gagtcgtgaa agctggcgtt ctggcgctcc agggcgactt ccgcgaacac gcgcgcgtgc
     2941 tcgaggacct cggcgcgacg ccggtcctcg tgcggaccgt cgacgagctc ggcgaggtct
     3001 cctgtctcgc gatccccggt ggcgagagca cgacgatgtc gaagctcgcg cggcgctacg
     3061 ggctcgtcga gccgatccgc gaacgcgcgt cccgtgggat gccggttttc gggacgtgtg
     3121 cgggcatgat cgtgatggcc cgcgaggt
//
LOCUS       DASZOB010000264         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_96526, whole genome shotgun sequence.
ACCESSION   DASZOB010000264 DASZOB010000000
VERSION     DASZOB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96526"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VG709_04305"
     CDS             complement(<1..1315)
                     /locus_tag="VG709_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWC32330.1"
                     /translation="MPRTSELRRNGAAEHAAHDLELVLETAHEAFVSMDEDGLVSAWN
                     RAAERTFGWPKEAALGRPLRDLVIPERYRRRHDEGLRRFLETGEGPLLDRRVEISALH
                     RSGHEFPVELTISAVQQAGTWRFHAFVHDISERYRANELQARLATLVEHSADAIVTRT
                     ADGVVTSWNPAAEELFGYPAEEMIGRTLDVLVPEARAGEARELIARVVAAEPVRGFET
                     QRVRKDGRVIDVSITISPIRDDAGAVRELSMIARDVTSRKESERELQRGYRELERLNE
                     LKSHFVAVASHELRTPLTSIRGFASTLLNRWGSLADEDKRTFLGHIEGQSERLTRIVG
                     EILTLARIEGGKLPVSPERVDVGLVARRVVAEQGVDGTVDVAGDEPAVVTADPDHVHR
                     ILLNLVVNAHAHGSPPYRISIGGDEHEVVVRVSDEGAGVPEALVGS"
BASE COUNT          196 a          567 c          516 g          231 t
ORIGIN      
        1 ggctgccgac gagcgcttcc gggacgcctg cgccctcgtc cgacacgcgg acgacgacct
       61 cgtgctcgtc gccgccgatc gagatccggt agggcggcga gccgtgagcg tgcgcgttga
      121 cgacgaggtt cagcaggatc cggtggacgt ggtcggggtc cgccgtcacc acggcaggct
      181 cgtccccggc gacgtcaacc gtgccgtcga ccccctgctc ggcgacgacg cgccgcgcga
      241 cgagacccac gtcgacgcgc tccggcgaca cggggagctt cccgccctcg atccgtgcga
      301 gcgtcaggat ctcgccgacg atccgcgtca ggcgctcgct ctgcccctcg atatggccga
      361 ggaaggtgcg cttgtcctcg tccgcgagcg agccccagcg gttgagcagc gtgctcgcga
      421 acccgcggat cgaggtgagc ggcgtgcgga gctcgtgcga cgcgacggcg acgaagtggc
      481 tcttgagctc gtttagccgc tcgagctctc tgtatccgcg ctggagctcc cgctcgctct
      541 ccttccgcga cgtgacgtcg cgcgcgatca tcgagagctc gcgcacggcg cccgcgtcgt
      601 cgcggatcgg cgagatcgtg atcgagacgt cgatcacgcg cccgtccttg cgcacccgct
      661 gcgtctcgaa gccgcggacc ggctccgccg cgaccacgcg ggcgatcagc tcgcgtgctt
      721 ccccggcacg agcctcgggc acgagcacgt cgagcgtgcg accgatcatc tcctcggccg
      781 gatacccgaa gagctcttcc gcggccggat tccagctcgt gaccacgccg tccgccgtgc
      841 gcgtgacgat cgcgtccgcc gagtgctcga cgagcgtcgc gagccgcgcc tggagctcgt
      901 tcgcccggta gcgctcgctg atgtcgtgga cgaaagcgtg gaagcgccat gtcccggcct
      961 gctgaacggc cgagatcgtg agctcgaccg gaaattcgtg cccgcttcgg tggagcgcgc
     1021 tgatctccac gcgcctgtcg aggagcggcc cctcgcccgt ctccagaaat cgccggagcc
     1081 cctcgtcgtg gcgccgccga tatcgctcgg ggatgacgag atcgcgaagc ggccggccga
     1141 gcgcggcctc cttgggccag ccgaacgtcc gctcggccgc gcggttccag gccgagacga
     1201 ggccgtcctc gtccatcgag acgaacgcct cgtgggcggt ctcgaggacg agctcgaggt
     1261 cgtgcgccgc gtgctcggca gccccgtttc gccgcagctc gctcgtgcgt ggcacgcggc
     1321 cgccgagccg acgctctcga tccccttcca ccggcgccac cttcgatgcc ctcgcttccg
     1381 gcggcggcaa gccgccccaa cgcttcgacg atcggccgct gcaggcctct cctcccccga
     1441 cgggccgacg ggctcggatc ttcccgctcg tccgccggta cggccgcatg tcctggtggc
     1501 ggacagggag
//
LOCUS       DASZOB010000265         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_12726, whole genome shotgun sequence.
ACCESSION   DASZOB010000265 DASZOB010000000
VERSION     DASZOB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12726"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VG709_04310"
     CDS             <1..350
                     /locus_tag="VG709_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWC32331.1"
                     /translation="SVVYTVCAVVLLVAALVSGTPLSGFAADTWLFMVLMALGPQIGG
                     HTTFNFLLRDIDATVVAVAVMAEPVGASLLALLLFGEVPPAVAIVGGAVILAGIYIAV
                     TAGTRRQLVPPVE"
     gene            361..2490
                     /locus_tag="VG709_04315"
     CDS             361..2490
                     /locus_tag="VG709_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWC32332.1"
                     /translation="MAETPAAVDIRDVQVIKDNRTFFLSDRFGDVPRSNTAALGLYHM
                     DTRFLSALEITVNDLKPLLLHSSTERNYSQIVELSYPFQMVDPRGMERKENVSLQRFR
                     VLSEALFERVRIRNFGSRIHRFSLRVEFAADFKDIFEVRGIVRERRGQVQSPRVDRNR
                     VVLSHRGLDGAVRTTTLRFSPAPDQIDETHAVFHVEVKEGGAAEIAVEIVPEVGSSPA
                     ARRSMKDAEERLARDYNGWRKRCTRLKASHPQLSSFLDRAVLDLRMLTSTDEGGAEYL
                     DAGVPWYSALFGRDALITAYQALGVNPQLAWGTLRGLAALQGKEKDDWREEEPGKILH
                     EVRVGELARAGEIPQTPYYGSIDSTPLWLITLHGAYRWTGDTDAVRELWPNVLAALEW
                     IDQYGDADGDGYLEYQRRSDRGLDNQGWKDSHDAICHPDGELAQGPIALVEVQGYVYQ
                     AKRDIARVARDIGETELADRLAREADELRERFNRDFWMEKEGFFCVALDGDKRQVPCI
                     TSNPGHALWSRIVDEEKAPRVVHKLTSPQLSSGWGIRTYSSRHRAFDPIGYHTGTVWP
                     HDNSLIAHGMKRYGFDREARKVLDQLALAGAFFPLARFPELFCGFSAEDVPVPVQYPG
                     ACRPQAWASGAPLLMVRSYGGLSADAPNNRLFIVRPKLPEWLERVEIFGMRVGGSRID
                     LVFTSNDGVTATEVPRKEGDIEILIRQ"
     gene            complement(2491..>2693)
                     /locus_tag="VG709_04320"
     CDS             complement(2491..>2693)
                     /locus_tag="VG709_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32333.1"
                     /translation="TGLVTVPVGPTGKWGALRYLRSAMAEIRRTAGEPHLAFIVALAS
                     DGRVVSGRDPDAALFVLGYSVR"
BASE COUNT          406 a          931 c          949 g          407 t
ORIGIN      
        1 cgtcggtcgt ctacaccgtg tgtgcggtcg tcctcctcgt cgcggcgctc gtctcgggga
       61 cgccgctgtc ggggttcgcg gccgacacgt ggctcttcat ggttctcatg gcgctcgggc
      121 cgcagatcgg cgggcacacg acgttcaact tcctcctgcg cgacatcgac gcgaccgtcg
      181 tcgcggtcgc cgtgatggcg gagccggtcg gcgcgtcgct cctcgcgctg ttgctgttcg
      241 gcgaggtgcc gcccgcggtc gcgatcgtcg gaggcgcggt gatcctcgcc ggcatctaca
      301 tcgcggtcac cgccgggacg cggcgacagc tcgtgccgcc ggtcgagtag catccggaca
      361 gtggcggaga cgccggcggc cgtcgacatc cgcgacgtcc aggtcatcaa ggacaaccgg
      421 acgttcttcc tctccgaccg cttcggcgac gtcccccgca gcaacaccgc ggcgctcggg
      481 ctctaccaca tggacacgcg gttcctctcc gcactcgaga tcaccgtgaa cgacctgaag
      541 ccgctgctgc tgcattcgtc gacggagcgg aactactcgc agatcgtcga gctctcgtac
      601 ccgttccaga tggtggaccc gcgcggcatg gagcggaagg agaacgtctc gctccaacgg
      661 ttccgcgtgc tctccgaggc gctgttcgag cgcgtgcgca tccggaactt cggaagccgc
      721 atccaccggt tcagcctgcg cgtcgagttc gcggcggact tcaaggacat cttcgaggtg
      781 cgcgggatcg tccgcgagcg gcgcggccag gtgcagtctc cccgtgtcga ccgcaatcgc
      841 gtcgtcctct cgcaccgcgg gctcgacggc gccgtgcgga cgacgacgct ccggttttcg
      901 ccggcccccg accagatcga cgagacgcac gcggtgtttc acgtcgaggt gaaggaaggc
      961 ggcgccgcgg agatcgccgt cgagatcgtc cccgaggtcg gctcctcgcc ggccgctcgc
     1021 cgctcgatga aggacgcgga ggagcggctc gcgcgggact acaacgggtg gcggaagcgt
     1081 tgcacgcgct tgaaggccag ccacccgcag ctgtcgtcgt tcctcgaccg cgcggtgctc
     1141 gacctgcgga tgctgacctc gaccgacgag ggcggggccg agtacctcga cgcgggcgtt
     1201 ccgtggtact cggcgctgtt cgggcgcgac gcgctcatca cggcgtacca ggcgctcggc
     1261 gtgaatccgc agctcgcatg ggggacgctc cgcgggctcg ccgcgctcca gggcaaggag
     1321 aaggacgact ggcgcgagga ggagcccggg aagatcctgc acgaggtccg cgtcggcgag
     1381 ctggcgcgcg ccggagagat cccgcagacc ccgtactacg ggtcgatcga ctcgacgccg
     1441 ctgtggctga tcaccctcca cggcgcatac cgctggacgg gcgacaccga cgccgtcagg
     1501 gagctgtggc cgaacgtcct ggcggcgctc gagtggatcg accagtacgg cgacgccgac
     1561 ggcgacggct atctcgagta ccagcggcgg tcggacaggg ggctcgacaa ccagggatgg
     1621 aaggactcgc acgacgcgat ctgtcatccc gacggcgagc tggcgcaggg accgatcgcc
     1681 ctcgtcgagg tccagggcta cgtgtaccag gccaaacgcg acatcgcgcg ggttgcccgc
     1741 gacatcgggg agaccgagct cgccgaccga ctcgcgcgcg aggcagacga gctccgcgag
     1801 cgcttcaacc gcgacttctg gatggagaag gaggggttct tctgcgtcgc gctggacggt
     1861 gacaagcggc aggtgccctg catcacgtcc aaccccgggc acgcgctctg gtcacggatc
     1921 gtcgacgagg agaaggcgcc gagggtggtc cacaagctga cctcgccgca gctgtcctcg
     1981 gggtggggga tacgcacgta ctcgagccgg caccgcgcgt tcgacccgat cggctatcac
     2041 accggaacgg tctggccgca cgacaactcc ctcatcgcgc acggcatgaa gcgctacggc
     2101 ttcgaccgag aggctcggaa ggtcctcgac cagctcgcgc tcgccggcgc cttcttcccg
     2161 ctcgcgcggt tccccgagct cttctgcggg ttctccgccg aggacgtgcc ggtgccggtc
     2221 cagtacccgg gggcgtgccg cccgcaggcg tgggcgtcgg gcgccccgct gctgatggtg
     2281 cgttcgtacg gaggcctctc ggcggatgcg ccgaacaacc gcctcttcat cgtgcgcccg
     2341 aagctgcccg agtggctcga gcgcgtcgag atcttcggga tgcgcgtcgg gggctcgcgg
     2401 atcgacctcg tgttcacgtc gaacgacggc gtgaccgcga ccgaggtccc tcgcaaggag
     2461 ggcgacatcg agatcctgat ccggcagtag ctaccggacg ctgtagccga ggacgaagag
     2521 cgcggcgtcg ggatcccggc cgctcacgac gcggccgtcg gacgcgagcg cgacgatgaa
     2581 cgcgaggtgg ggctcgccgg cggtccgcct gatctcggcc atggccgagc gcaggtagcg
     2641 caacgcgccc cactttcccg tcgggccaac cggaacggtc accaggccgg taa
//
LOCUS       DASZOB010000266         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_185519, whole genome shotgun sequence.
ACCESSION   DASZOB010000266 DASZOB010000000
VERSION     DASZOB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185519"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VG709_04325"
     CDS             complement(<1..269)
                     /locus_tag="VG709_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWC32334.1"
                     /translation="MATAAHAHEHHPPVANVSSRVDARLLGIYIFIASEVMLFGSFFT
                     AYFFARVIVAPEQWPPEPFELPVYLALLNTIILVTSSFTMHWALQS"
     gene            complement(278..1111)
                     /locus_tag="VG709_04330"
     CDS             complement(278..1111)
                     /locus_tag="VG709_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32335.1"
                     /translation="MSTGPRHILVVANETVVGRKLLDAVRERAGRGPIRVTVVCPQNA
                     PRTGYVVYEDERRSAAERRLRRTLDLLHEAGIAARGAVVDPDPLQAIRDALHEYQPDE
                     VIVSTHPESQGSSWLRGNLVDRARKAAGDVPVEHVVVDLDERRERAHVLVIGSETVVG
                     EPVLAAVRERADQSPAEFTVVVPADPPGAERRMQRTLAQLRAAGIEATGHVGDPDPVS
                     AVVNAMHDEPVDEIIISTFPRATSGWLRRDVLGRIRKHTSVPISHVVVEREEAEAAVA
                     S"
     gene            complement(1152..>1696)
                     /locus_tag="VG709_04335"
     CDS             complement(1152..>1696)
                     /locus_tag="VG709_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWC32336.1"
                     /translation="SDTYFVVAHIHYVLLGGSLFTIMAGVYYWFPKMTGRMYDERLGK
                     LHFWLTCVAFNATFFPMHWIGSRGMPRRVADYEPVFANWNLVISIASFFLGASTLVFI
                     YNLLVSWRYGEPAPGNPWRALTLEWQVSSPPPIFNFDEIPQVVGSPYEYGVRGARHAI
                     VNGKPTTEGREAAPVREMVP"
BASE COUNT          266 a          572 c          612 g          246 t
ORIGIN      
        1 gactggagcg cccagtgcat cgtgaagctc gacgtcacga ggatgatcgt gttcaggagc
       61 gcgaggtaaa cgggaagctc gaacggctcc ggcggccatt gctcgggcgc gacgatcacc
      121 cgtgcgaaga agtaggcggt gaagaacgag ccgaagagca tcacctccga cgcgatgaag
      181 atgtagatcc cgagcagccg cgcgtcgacc cgcgagctga cgttcgcgac ggggggatga
      241 tgctcgtgtg cgtgcgcggc cgtggccatt gcgccggtta cgaggcgacc gccgcctcgg
      301 cctcctcgcg ctcgacgacg acgtggctga tcggcacgct cgtgtgcttg cgaatccgcc
      361 cgaggacgtc gcggcggagc cagccggagg tcgcgcgcgg aaacgtcgag atgatgatct
      421 cgtccacggg ctcgtcgtgc atcgcgttga cgacggccga gacggggtcg gggtcgccga
      481 cgtgtcccgt cgcctcgatg cccgccgcgc ggagctgggc gagcgtccgc tgcatcctcc
      541 tttctgcgcc gggagggtcg gccgggacga cgaccgtgaa ctcggcgggc gactggtccg
      601 cacgctcgcg aactgccgcc aggaccggct ccccgacgac ggtctcgctc ccgatcacca
      661 gtacgtgcgc ccgctcgcgc cgctcgtcga ggtcgacgac gacgtgctcg accggcacgt
      721 ccccggccgc cttgcgagcc cggtcgacga ggttgccgcg caaccagctc gagccctggc
      781 tctcgggatg ggtcgagacg atcacctcgt ccggctggta ctcgtgcagc gcgtcgcgga
      841 tcgcctgcag gggatcgggg tcgacgacgg cgccgcgtgc cgcgatcccc gcctcgtgga
      901 ggaggtcgag cgtccggcgc aggcgacgct cggccgcgga tcgccgctcg tcctcgtaga
      961 cgacgtagcc cgtgcgcggc gcgttctggg gacagacgac ggtgacgcgg atcggcccgc
     1021 gtccggcgcg ctcgcggacc gcgtcgagga gcttccgccc gacgacggtc tcattcgcga
     1081 cgacgagaat gtgacgcggt ccggtgctca ccggctctgc accgggccgg gccgacgcgc
     1141 ggcgtgcgcc ctcacgggac catctctcgg acgggagccg cctctcgacc ctccgtggtc
     1201 ggcttgccgt tcacgatcgc gtgccgggcg ccgcggacgc cgtactcgta cgggctgccg
     1261 acgacctgcg ggatctcgtc gaagttgaag atcggcgggg gcgaagagac ctgccactcg
     1321 agcgtcagcg cccgccacgg gttccccggc gccggctcgc cgtagcgcca gctcacgagc
     1381 aggttgtaga tgaacacgag cgtggaggcg ccgaggaaga acgacgcgat cgagatcacg
     1441 aggttccagt tcgcgaagac cggttcgtag tcggcgacgc gccgcggcat ccccctcgac
     1501 ccgatccagt gcatcgggaa gaaggtggcg ttgaaggcga cgcacgtcag ccagaagtgg
     1561 agcttcccga gccgctcgtc gtacatccgg ccggtcatct tcgggaacca gtagtagacc
     1621 ccggccatga tcgtgaagag cgagccgccc agcagcacgt agtggatgtg cgccacgacg
     1681 aagtacgtgt cggaga
//
LOCUS       DASZOB010000267         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_134019, whole genome shotgun sequence.
ACCESSION   DASZOB010000267 DASZOB010000000
VERSION     DASZOB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134019"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VG709_04340"
     CDS             complement(<1..545)
                     /locus_tag="VG709_04340"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HWC32337.1"
                     /translation="MISFRFHLVSLIAVFLALGLGIITGTTVINRAIVSQLEDQTNRL
                     VAQEAENRAAITRLQAEVDTATAFAEQAAGYLVDGALEQESVAVVTEEATDPDAITAA
                     QTALTEAGAEVVALLALDDALSALNESQRTQLGAALGFEADGDAGAFGAQIASALANR
                     LAFGVRPGQPDVLATLISEGYL"
     gene            complement(558..1661)
                     /gene="steA"
                     /locus_tag="VG709_04345"
     CDS             complement(558..1661)
                     /gene="steA"
                     /locus_tag="VG709_04345"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HWC32338.1"
                     /translation="MRLATRRTRRPPTVAVIDHEDLDRVAAEGLAARGVSAVVNASAS
                     ITGRYPNLGPLVLVRAGIPLIDNVGTLLMDKVREGSPVRIDGDRVYVDDDLVGVGIRQ
                     TEASVLRDMDDARTGLEHQFEGFARNTLEYMQRERDLLFGGGGMPDLDEDLAGRHVLV
                     VVRGAGYREDLAALRAYVRDVRPVLVGVDGGADALLEQGYRPNVIVGDMDSVSEQALT
                     SGAEIVLHAYPGGHAPGRERLESLGLEYRDVEASGTSEDLAYLLAHEKGAALIVAVGS
                     HGNLREFLDKGRRGMASTFLVRLRVGEILVDAKGVSRMYPGGVRGRDVALLVGAALFS
                     IVAVVAISAPMQLFFRQLVEMGRQFLFRIGQLF"
     gene            1773..2153
                     /locus_tag="VG709_04350"
     CDS             1773..2153
                     /locus_tag="VG709_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWC32339.1"
                     /translation="MRRLMMVLAASVALVLAAAGPSVAQESGPTPGPTPYYDFPTVGP
                     TPYFECPTVGTIPYDVAPLPYEVGPTPYYVGPRPYFVVCEAATGGGASRPLPQTGTSL
                     ALIAGAGVVVMGLGLWAARRRAAS"
BASE COUNT          318 a          794 c          754 g          319 t
ORIGIN      
        1 aggtacccct cgctgatgag ggtcgcgagc acgtcaggct gtccgggccg caccccgaac
       61 gccaatcggt tcgcgagcgc cgatgcgatc tgggccccga acgcgcctgc gtcgccgtcg
      121 gcctcgaacc ccagcgcagc gccgagctgc gtgcgttgag actcgttcag ggcggagagg
      181 gcatcgtcga gcgcgaggag cgcgaccacc tcagctcccg cctccgtcag cgcggtctgc
      241 gccgcggtga tcgcatcggg atcggtcgcc tcctcggtca cgacggcgac gctttcctgt
      301 tccaacgcgc cgtcgacgag atagcccgcc gcctgctccg cgaaggccgt cgccgtgtcg
      361 acctcggcct gcagccgcgt gatcgcggca cggttctccg cttcctgcgc gacgaggcgg
      421 ttcgtctggt cctcgagctg cgaaacgatc gcgcggttga tcacggtcgt ccccgtgatg
      481 atgccgaggc ctagtgcgag gaagaccgcg atcagcgaga cgagatggaa gcggaacgag
      541 atcaccggga ggccagccta gaacagctgt ccgatgcgga acaggaactg ccgacccatc
      601 tccacgagct gccggaagaa gagctgcatc ggcgcggaga tcgcgaccac cgccacgatc
      661 gagaagagcg ctgcgccgac gagtagcgcg acgtcgcggc cccggacgcc gccgggatac
      721 atgcgcgata cccccttggc gtccacgagg atctccccga cccgaaggcg gacgaggaaa
      781 gtgctcgcca tgccgcggcg ccccttgtcg aggaactcgc ggaggttccc gtggctgccg
      841 accgcgacga tcagcgcggc gcccttctcg tgcgcgagca ggtacgcgag gtcctcgctc
      901 gtcccggagg cctcgacgtc cctgtactcg aggccgagcg actcgagccg ttcgcgcccg
      961 ggtgcatgtc cgccgggata cgcgtggagc acgatctcgg cgcccgaggt gagcgcctgc
     1021 tccgacacgc tgtccatgtc gccgacgatc acattgggcc ggtacccctg ctcgagcagc
     1081 gcgtcggcgc ccccgtcgac cccaacgagc accggcctga cgtcgcgaac gtacgcccgg
     1141 agcgcggcga gatcctctcg gtagcccgct ccgcggacga cgacgaggac gtgccgcccg
     1201 gcgaggtcct cgtccagatc cggcatgccg ccgccgccga acaggagatc gcgctcgcgc
     1261 tgcatgtact ccagcgtgtt ccgcgcgaac ccctcgaact ggtgctcgag gccggtcctg
     1321 gcatcgtcca tgtcccgcag caccgacgcc tcggtctgcc ggatccccac cccgacgagg
     1381 tcgtcgtcga cgtacacgcg gtcgccgtcg atccggacgg gtgaaccttc acgcaccttg
     1441 tccatgagga gcgttccgac gttgtcgatc agcgggatcc cggcgcgcac gagcacgagg
     1501 ggcccgaggt tcggatatcg gccggtgatc gaggcgctcg cgtttacgac cgcagagacg
     1561 ccgcgcgcgg cgagcccctc ggcggcgacg cgatcgagat cctcgtggtc gatgacggcg
     1621 acggtcggcg gtcgccgggt ccgtcgcgtg gcgagccgca acctggacct ccgtttctcg
     1681 tgatccagcc cagggtaccg ccctggcggc tcgcgatccg tggggctccg gtgatagcgt
     1741 tcccgcgcat cgcccgttgg agggaggagc cgatgagacg gctcatgatg gtcctcgccg
     1801 cgtcggtggc gctcgtactg gccgcggcgg gtccgtcggt ggcccaggag agcggcccga
     1861 cccccggccc cacgccgtac tacgacttcc cgacggtcgg gccgacgccg tacttcgagt
     1921 gcccgacggt gggcacgata ccgtacgacg tggccccgct accgtacgag gtcggcccga
     1981 ccccctacta cgtggggccg cggccctact tcgtcgtatg cgaggccgcc accggaggcg
     2041 gcgcatcccg cccccttcct cagacgggga cgtcgctcgc gctcatcgcg ggtgccgggg
     2101 tcgtggtcat gggcctgggg ctgtgggcgg cccgtcggcg cgccgcgtcc tgatcgcgac
     2161 ccgcgcgaag ccgcaccacg cggcg
//
LOCUS       DASZOB010000268         6568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_115875, whole genome shotgun sequence.
ACCESSION   DASZOB010000268 DASZOB010000000
VERSION     DASZOB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115875"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1393)
                     /gene="dnaB"
                     /locus_tag="VG709_04355"
     CDS             complement(50..1393)
                     /gene="dnaB"
                     /locus_tag="VG709_04355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWC32340.1"
                     /translation="MARAAEDLRAPRPDRVPPHNLDAEQSVLGAMMLSGDAIAQVIDV
                     GIGADDFYKRSHGGIFDALTALYARGEPVDQVTAVEELRRRGTLDGAGGPLYIHHLVS
                     TVGTPASAGHYARIVADHALLRRLISAASDVIDGAFEVPEDPEGFADHAEGRIYQVSR
                     RHERDQIVPLRELVREGLEDIERIHERTGLVGIPTGFRDLDDLLQGLQRGNLIVVAAR
                     PGIGKSSLVTNIARHAAVKERVPVSLFSLEMSRTEIGMRLLCTDARVPWQKIRAGKIG
                     GDDWGRIVEAAGALDPAPLYIVDSGNVTIVDIRAKARRMKSQHGLGLIIVDYLQLMSS
                     HRRVDNRQQEVAEISRSLKLLAKELDVPIIAVSQLNRDPERRTDKKPQLADLRESGAI
                     EQDADVVIFIHRDPLSEDSSVAEVIVAKHRNGPLGRITLTWLEHLTLFQNHAAQR"
     gene            complement(1580..2026)
                     /gene="rplI"
                     /locus_tag="VG709_04360"
     CDS             complement(1580..2026)
                     /gene="rplI"
                     /locus_tag="VG709_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWC32341.1"
                     /translation="MRIILQKEVEKLGAPGDIVQVADGYARNYLIPRGFAVPASKGAV
                     RHSEKLKAGHDERQRRARAEAQELATRLAKTPVRIQARAGEEGRLFGSITAQEIADEL
                     SRALEQPIDRRRMSLDEPIRSIGVHSVRVHLHPEVDANVTVDVVAQ"
     gene            complement(2023..2307)
                     /gene="rpsR"
                     /locus_tag="VG709_04365"
     CDS             complement(2023..2307)
                     /gene="rpsR"
                     /locus_tag="VG709_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWC32342.1"
                     /translation="MARGKQGGKPPRKPRREKADDAWQKKQRKKFCLFCRDKVDYVDY
                     KDTGTLRRFISDRGKIRARRVTGTCSQHQRDVAAAVKNAREMALLPYSAR"
     gene            complement(2335..2778)
                     /gene="ssb"
                     /locus_tag="VG709_04370"
     CDS             complement(2335..2778)
                     /gene="ssb"
                     /locus_tag="VG709_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWC32343.1"
                     /translation="MANENQVMIVGNLTDDPELRYTPNGAAVANFRVAVSRRVKDDAT
                     GQWKDADTSFFRVSAWRSLGENVAESLTRGSRVVVVGRLRSRSWETPEGETRSTVEIE
                     ADEIGPSLRWATAKPEKTQRSAGDWSDNVSVGAGGGGKETPLETP"
     gene            complement(2842..3339)
                     /gene="rpsF"
                     /locus_tag="VG709_04375"
     CDS             complement(2842..3339)
                     /gene="rpsF"
                     /locus_tag="VG709_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWC32344.1"
                     /translation="MRSILRPVSRVSSSTGAMPRSSSRPGSTDAPGCHTPAVRSLRPG
                     PGAAAARLRTFPPLLPLGARDQRRWHLRQYELMVIVDPAADEEKVGTVVDRVTKILTD
                     RGGEVSGVDRWGRRKLAFEIDRKSEGYYLVLRMNADPEILAELDRVLSVADEVLRFKV
                     VRTAA"
     gene            complement(3254..4372)
                     /locus_tag="VG709_04380"
     CDS             complement(3254..4372)
                     /locus_tag="VG709_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32345.1"
                     /translation="MTEQNVRDRLTPVLDRLPVDIERALEVVARRATRRTRMARAATI
                     AVAAVVAAVGVVAIWRLSGLRDDAPRPGSVGARGKISYIRVAVEGEQQTVEGFVAAPD
                     GSGRIPLITDSEWELFPAWSPDGRRVAYAGGPSDDVERTNLYLAAPDGTAGRKLVDGG
                     VTGPMAWSPDGTHIAFIRRDPFPGEDALWIVQSDGTGARRVLDGRWNNVAWSPKGNRL
                     ALGGDPGGGDVADAEGIYVVGADGQGLRRVTPAEDGFGFFPTWSPDGSRIAFVRNQDY
                     DDIDPKSDIYVVDIDGGAAERLTQWEGLDTIPVWSPDGGELLFTSDRGVSDAERRQNM
                     EAGELSGSSIYALDLETGVTRLVIDGGDAAVFVTSWVD"
     gene            complement(4369..4890)
                     /locus_tag="VG709_04385"
     CDS             complement(4369..4890)
                     /locus_tag="VG709_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC32346.1"
                     /translation="MKAGTVPRERPIERLADFDSFFRDEFPRVVRAAALVARDLGAGE
                     DCAQEAFLRLHQRWGRMESDAHARNFAYRAALNLARSHVRKHLRVSLSGSTHETAQSD
                     ASSATVEWLALLDAVASLSGKQRAVLALVDYLGYDADAAARTLGISPSTVRVHLKRAR
                     DAVRRHLTGGDPR"
     gene            complement(4939..6009)
                     /locus_tag="VG709_04390"
     CDS             complement(4939..6009)
                     /locus_tag="VG709_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWC32347.1"
                     /translation="MRASLRRTGYVVVVLTGLALLPPVQARAKALAVVVEAIGLPFTP
                     RPFAAPVTRSETTLGGVRGDVYDPGGDAPAILLVHGAALQGKDDPRLVRLAQAVSRAR
                     RLVFVPALTLAERRFDAGDIERIVRSGAALAERVGAPISVLGISYGGSFALIAAADER
                     LEDRLEQVAVFGAYYDLRGVIQAVTAGEALVAGERIPWRGDPQAIEILERHAVQLAPE
                     EAQARLSAALDGAGDSSALPPDARAVYELLSNRDPTRTYELASRLSPTAREILERFSP
                     SSVADRIRVPIVAIHSKDDPAVPYGEALRLVRALPDTPLETLGSFRHVDFELGSPRTW
                     GRAAGDLWSTLRFTTWLLEPAE"
     gene            complement(6046..>6568)
                     /gene="panB"
                     /locus_tag="VG709_04395"
     CDS             complement(6046..>6568)
                     /gene="panB"
                     /locus_tag="VG709_04395"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWC32348.1"
                     /translation="VSTEEAIRGAGRFLKEGGMHAIKIEGPHLELAEALTSRGIPVRG
                     HLGLTPQSVHAMGGYRVQAKSEEAADRLLADAMALEKAGVFSLVLEGIPAGVAKRVTE
                     SVRVPTIGIGAGPHCDGQVLVLTDLLGLGEGKYPKFVKPFANLREEIARAVRAFADEV
                     EAGTYPDEDHSYR"
BASE COUNT          931 a         2359 c         2265 g         1013 t
ORIGIN      
        1 tcgcggacgt ccgggggcgc ggagggcttc cgcgaggcgc gccgcctccc taccgctggg
       61 cggcgtggtt ctggaacagc gtcaggtgtt cgagccacgt gagcgtgatt ctgccgagtg
      121 gcccgttgcg gtgcttggcg acgatcacct cggcgacgga ggagtcctcg gacagcgggt
      181 cgcggtggat gaagataacg acgtcggcgt cctgctcgat cgcgccggac tcgcgcaggt
      241 ccgcgagctg cggcttcttg tcggtccgcc gctcggggtc acggttcagc tgcgagaccg
      301 cgatgatcgg cacgtcgagc tccttggcca ggagcttcag cgagcgcgag atctcggcga
      361 cctcctgctg gcggttgtcc acgcggcggt ggctcgacat cagctggagg tagtcaacga
      421 tgatcagccc gagcccgtgc tggctcttca tccgccgcgc cttcgcgcgg atatcgacga
      481 tcgtcacgtt cccgctgtcg acgatgtaga gcggcgccgg atcgagcgcg ccggctgcct
      541 cgacgatccg cccccagtcg tcgcctccga tcttcccggc gcggatcttc tgccagggga
      601 cgcgcgcgtc cgtgcagagg aggcgcatgc cgatctcggt gcgggacatc tccagggaga
      661 acagcgagac gggtacccgc tctttgaccg cggcgtgacg ggcgatgttc gtgacgagcg
      721 acgacttccc gatgccggga cgggcggcga cgacgatgag gttgcccctc tgcagcccct
      781 ggaggaggtc gtcgaggtcg cggaacccag tcgggatccc cacgagccct gtgcgctcgt
      841 ggatccgctc gatgtcctcg agcccctctc tgacgagctc gcggagcggg acgatctgat
      901 ctcgttcgtg acgccgcgag acctggtaga tgcgcccctc ggcgtggtcg gcgaacccct
      961 ccgggtcctc ggggacctcg aaggcgccgt cgatgacgtc tgaggccgcc gagatgaggc
     1021 ggcgcagcag cgcgtggtcc gcgacgatcc gcgcgtagtg gccagcgctg gccggcgtgc
     1081 cgacggtcga gaccaggtgg tggatgtaga gcggcccccc ggcgccgtcc agggtcccgc
     1141 gtcgccggag ctcctcgacc gccgtcacct ggtcgacggg ctcgccgcgc gcgtagaggg
     1201 ccgtcagcgc gtcgaagatc ccgccgtggg accgcttgta gaagtcgtcg gccccgatgc
     1261 cgacgtcgat gacctgcgcg atcgcgtcgc ccgagagcat catcgccccg aggaccgatt
     1321 gctcggcgtc gaggttgtgt ggcgggaccc ggtccggacg cggcgcccgg aggtcctcag
     1381 cggctctcgc catgccccct ccccatgacg agcgcggact ctagggacag cggcctgtgg
     1441 aactcaagga ggacgacctc gggagtccgt gtggaaaggt cacgaactcc gaggtcggac
     1501 caaggaaata gttggcgcac tttcttcgcc cccatgcgtt gagcatgacg ggggggtctg
     1561 acacgcgctc gagagcgcgc tactgcgcga cgacgtccac ggtcacgttc gcgtcgacct
     1621 cgggatggag gtggacgcgg acggagtgga cgccgatgct tcggatcggc tcgtcgaggg
     1681 acatcctgcg gcggtcgatg ggctgctcca gcgcacgcga gagctcgtcg gcgatctcct
     1741 gggccgtgat gctgccgaac agccggccct cctcccccgc acgggcctgg atccggaccg
     1801 gcgtcttcgc gagccgggtc gcgagctcct gcgcctccgc cctcgcgcgc cgctgccgct
     1861 cgtcgtggcc ggccttgagc ttctccgagt ggcggaccgc ccccttgctc gccggaacgg
     1921 cgaacccgcg cgggatgagg tagttgcgcg cgtagccgtc ggctacctgg acgatgtcgc
     1981 ccggtgcccc gagcttctcg acctctttct gaaggatgat cctcatcgcg ccgaatacgg
     2041 caggagcgcc atctcgcgcg cgtttttcac ggccgctgcg acgtcccgct ggtgctgcga
     2101 gcacgtgccg gttacccgcc gagcgcggat cttgccgcga tcggagatga accgccgcag
     2161 cgtgcccgtg tccttgtagt cgacgtagtc gaccttgtcg cggcagaaga ggcagaactt
     2221 cttgcgctgc ttcttctgcc aggcgtcgtc ggccttctcc cggcgaggct tccgtggcgg
     2281 cttaccgcct tgctttccgc gtgccagctc ggttccctcc ttctcgtagt cgtgctacgg
     2341 cgtctcgagc ggcgtttcct tgccgccgcc gcccgcgccc acgctcacgt tgtcgctcca
     2401 atcgcccgcc gagcgctggg tcttctccgg cttcgcggtc gcccaccgga ggctcggacc
     2461 gatctcgtcg gcctcgatct cgacggtcga gcgagtctcg ccctcgggcg tctcccacga
     2521 ccgcgagcgg agcctcccga cgacgaccac gcgcgacccg cgggtcagcg actccgcgac
     2581 gttctcgccg agcgagcgcc acgcgctcac gcggaagaac gacgtgtcgg cgtccttcca
     2641 ctgcccggtc gcgtcgtcct tcacgcgacg gctcaccgcg acgcggaagt tcgcgacggc
     2701 cgccccgttc ggcgtgtacc tgagctccgg gtcgtcggtc aggttgccga cgatcatgac
     2761 ctggttctcg ttcgccatct cgcttcacca tcccgcctgc tcgtcccccg ccggtgcgcc
     2821 cgcggaggag gggaagttcc gctatgccgc cgtccgcacg accttgaacc gcaggacctc
     2881 gtccgcgacg gagaggacgc ggtcgagctc cgcgaggatc tcggggtcgg cgttcatccg
     2941 gaggacgaga tagtagccct ccgacttccg gtcgatctcg aaggcgagct tgcgccgtcc
     3001 ccagcgatcg acgccagaca cctcgccgcc tcggtcggtg aggatcttcg tcacgcggtc
     3061 gacgaccgtg ccgaccttct cctcgtcggc cgccgggtcg acgatgacca tgagctcgta
     3121 ctgcctcaga tgccacctcc tctggtctct ggcccccagg gggagcaggg gtgggaaggt
     3181 cctcagtcta gcagccgcgg ctcccggccc cggtctaagc gaccgtaccg caggggtatg
     3241 acatcccgga gcgtcagtcg acccaggacg tgacgaagac cgcggcatcg cccccgtcga
     3301 tgacgagacg cgtgacaccg gtctcaagat cgagcgcata gatggaagac cccgagagct
     3361 ccccggcctc catgttctgg cggcgctcgg cgtccgagac cccacggtcg ctcgtgaaca
     3421 gcagctctcc gccgtcggga gaccagaccg ggatggtgtc cagcccctcc cactgggtga
     3481 gacgctcggc tgccccgccg tcgatgtcaa cgacgtagat gtccgacttc gggtcgatgt
     3541 cgtcgtagtc ctggttccgc acgaaggcga tccgtgatcc atccggcgac cacgtcggga
     3601 agaaaccgaa cccgtcttcg gcgggcgtga cgcgccgcag cccctggccg tccgcgccga
     3661 cgacatagat cccttcggcg tcggcgacgt cgccgcctcc ggggtcgccg cccaacgcga
     3721 gtcgatttcc cttcggcgac cacgcgacgt tgttccagcg gccgtcgagc acacgacgag
     3781 cgccggtccc gtcactctgc acgatccaga gcgcatcctc accggggaaa ggatcgcgac
     3841 gtatgaatgc gatgtgcgtt ccatcgggcg accacgccat cggcccggtc actccgccgt
     3901 cgacgagctt gcggccagcc gttccgtcgg gggccgccaa gtacaagttc gtccgttcca
     3961 cgtcgtcgga cggcccgccg gcgtacgcca ctcggcgccc gtcgggcgac catgccggaa
     4021 acagctccca ttcggaatcc gtgatcagcg gtatgcgccc ggatccgtcc ggtgccgcta
     4081 cgaacccctc gacggtctgc tgctcaccct cgaccgcgac tcggatgtac gagatcttgc
     4141 cgcgcgcacc gacgctcccg ggacgggggg cgtcatcgcg gagccccgag agccgccaga
     4201 tcgccaccac gccaacggct gcaaccaccg ccgccaccgc gatcgtcgcg gcgcgggcca
     4261 tacgtgtccg ccgggttgcg cgacgcgcga cgacctcgag cgcccgctcg atgtcgacgg
     4321 ggagccggtc gaggaccgga gtgaggcggt cgcggacgtt ctgttctgtc atcgcgggtc
     4381 ccctccggtg agatgtcgac gcacggcgtc gcgggcgcgc ttgaggtgaa cccgcaccgt
     4441 cgacggggag atcccgagcg tccgtgcggc ggcatcggcg tcgtacccga ggtagtcgac
     4501 gagggccagc accgcgcgct gcttccccga cagcgaggcg accgcgtcga gcaatgcgag
     4561 ccactcgacc gttgccgagg atgcgtccga ctgcgccgtc tcgtgcgtcg agcccgagag
     4621 gctcacgcgg aggtgcttcc gcacgtgaga gcgcgcgagg ttgagcgcgg ctcggtaggc
     4681 gaagttccgc gcgtgagcgt cggactccat ccgtccccat cgctggtgca gccgcaagaa
     4741 cgcctcctga gcacagtcct cgccggcacc gagatcgcgc gccacgagcg ccgcggctcg
     4801 aacgactcgc gggaactcgt cgcggaagaa cgagtcgaag tcagcaaggc gctcgatcgg
     4861 ccgctccctt gggacggtgc cggccttcat cggtctagct acgcgcgacg gcgcgcgcgc
     4921 gtttacacgt cgggacgttc actcagccgg ctcgaggagc cacgtcgtga accgcagggt
     4981 cgaccagagg tcgccggccg cgcggcccca cgtgcggggc gatccgagct cgaagtcgac
     5041 gtgtcggaac gatccgagcg tctcgagtgg cgtgtccggc agcgcgcgga cgagccgcag
     5101 cgcctcgccg tacgggaccg ccgggtcatc cttggagtgg atcgcgacga tggggacacg
     5161 gatgcggtcc gcgaccgagg acggcgagaa gcgctcgagg atctcgcgtg ctgtcggcga
     5221 cagccgcgac gccagctcgt acgtccgggt cgggtcgcgg ttcgacagca gctcgtacac
     5281 ggcgcgggcg tcgggcggga gcgccgacga atcgccggcg ccatcgagcg ctgcggagag
     5341 ccgtgcctgc gcctcctcgg gcgcgagctg gacggcgtgg cgctcgagga tctcgatcgc
     5401 ctgcgggtct ccgcgccacg ggatccgttc gccggcgacg agcgcctccc ccgccgtgac
     5461 ggcctggatc acgccgcgga ggtcgtagta cgcgccgaac accgccacct gctccaggcg
     5521 gtcctcgagc cgctcgtcag cggcggcgat gagcgcgaac gagcctccgt acgagatgcc
     5581 gagcaccgag atcggggcgc ccacgcgctc cgcgagggcc gcgcccgatc gcacgatccg
     5641 ctcgatgtcg ccggcgtcga accgccgttc cgcgagcgtc agcgccggga cgaagacgag
     5701 acgccgcgcg cgggagaccg cctgcgccag ccgaacgagc cgcgggtcgt ccttcccctg
     5761 gagtgccgcg ccgtggacca gcaggatcgc cggcgcgtcg ccgccgggat cgtagacatc
     5821 gccgcgtacg ccgccgagcg tcgtctccga ccgcgtgacc ggcgccgcga acggcctcgg
     5881 cgtgaacggc agcccgatcg cctccacgac gacggcgagc gccttcgccc gcgcctgcac
     5941 cggcgggagc agcgcgaggc ccgtcaggac gacgacgacg tatcccgtcc gccgcagcga
     6001 cgcacgcacg ggtgcgagcc tacgtgcgcg aagcggcccg cgcagttacc ggtacgagtg
     6061 gtcctcgtcc ggatacgtgc cggcctcgac ctcgtcggcg aaggcccgga cggcgcgcgc
     6121 gatctcttcc cgcaggttcg cgaacggctt cacgaacttc gggtacttcc cctcgccgag
     6181 cccgagcaga tcggtcagca cgagcacctg cccatcgcag tgcggccccg cgccgatccc
     6241 gatcgtcggg acgcggaccg actccgtgac gcgcttcgcg acgccggcgg ggatgccctc
     6301 gaggacgagc gagaagacgc cggccttctc gagcgccatc gcatcggcga gcagacggtc
     6361 cgcggcctcc tcgctcttcg cctggacgcg gtacccgccc atcgcgtgga ccgactgcgg
     6421 cgtgagcccg aggtggcccc tgaccgggat gccgcgcgag gtgagcgcct cggcgagctc
     6481 gaggtgcggg ccctcgatct tgatcgcgtg catcccgcct tccttgagga agcggccggc
     6541 gccccggatc gcctcctccg tcgacacc
//
LOCUS       DASZOB010000269         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_107050, whole genome shotgun sequence.
ACCESSION   DASZOB010000269 DASZOB010000000
VERSION     DASZOB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107050"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VG709_04400"
     CDS             <1..506
                     /locus_tag="VG709_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWC32349.1"
                     /translation="VQHLGEPWQIAILLAVFGVVLWIADRSEASRRIGEMTLRDAVAV
                     GLVQTLALAPGVSRSGVTISAARFLRLDRDSAARFSFLLLLPTTLGAVVWKGITDVVL
                     ADLPPGSTGPFVVGTIAAAVSGLFAIWALLGYVRRRTYSVFVVYRLVAAAVVLLLIVS
                     GTREATF"
     gene            564..1628
                     /locus_tag="VG709_04405"
     CDS             564..1628
                     /locus_tag="VG709_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWC32350.1"
                     /translation="MRETARRAFVAAIVIGAVVVGALALWKLKLVISVLFLAFIIAAA
                     MRPGVEALRRRRVPRGVGVLVHYLVLFALVGLLFYFVVPAASNQIREAVPTSSSELET
                     AAQESEGVKQRVLLEVQERLNRLPSADEFAERAVDPAVEITVRAFEIMVGIFFTLACA
                     AYWIFERDRAVNLVTSLLPRSRRKMVSDTWDLIDAKLGAYVRGQLLLIAFVATMLSVI
                     FWAIDLPFWLLLAIFAGVVEIIPVIGPRAAGALAVGVGLTASWKVALAAGIAVLVVRL
                     FEDYVVIPRVLGDAVGLTPLTVLVAVSSVAILFGGFAVLLAIPLAAVLATLIDVLIRH
                     RDPSREDVPTVLFPAKDVEA"
BASE COUNT          194 a          621 c          554 g          318 t
ORIGIN      
        1 tcgtccagca cctcggagag ccctggcaga tcgcgatcct cctcgccgtg ttcggcgtcg
       61 tcctctggat cgcggatcga tcggaggcga gccggcgcat cggcgagatg acgcttcgcg
      121 acgcggtcgc ggtcggcctc gtgcagacgc tggcgctcgc gccgggcgtc tcgcgctccg
      181 gcgtcacgat ctcggctgcc cggttcctcc ggctcgaccg cgactcggcc gcccgcttct
      241 cgttcctcct cctgctgccg acgaccctgg gcgccgtcgt ctggaagggg atcacggacg
      301 tcgtcctcgc cgatctcccg cccgggtcga cgggcccgtt cgtcgtcggg acgatcgccg
      361 ccgccgtgag cgggctcttc gcgatctggg cgcttctcgg ctacgtgcgc cggaggacgt
      421 actcggtgtt cgtcgtctac cgcctcgtcg cggccgccgt ggtcctgctc ctgatcgtct
      481 ccggcacgcg cgaggcgacg ttctagaaat ccggcaggcc cggcgcgcgt ctgctccaat
      541 aggcgcgagg cgaaaagagt cgaatgcgcg aaaccgcccg ccgagccttc gtcgccgcca
      601 tcgtgatcgg cgcagtggtc gtgggggcgc tggcgctctg gaagctcaag ctcgtcatct
      661 ccgtcctctt cctcgccttc atcatcgccg ccgcgatgcg gcccggcgtc gaggcgctca
      721 ggcgacggcg cgtgccgcga ggggtcggcg tcctcgtgca ctacctcgtg ctcttcgcgc
      781 tcgtcggcct cctcttctac ttcgtcgtcc cggccgcgtc gaatcagatc cgcgaagccg
      841 tcccgacgtc gagctcggag ctcgagacgg cggcacagga gtccgagggc gtcaagcagc
      901 gagtcctcct agaggtgcag gagcgcctca accggcttcc gagcgccgac gagttcgccg
      961 agcgtgccgt cgacccggcg gtcgagatca cggtgcgcgc gttcgagatc atggtcggga
     1021 tcttcttcac gctggcgtgt gccgcctact ggatcttcga acgcgaccgg gcggtcaacc
     1081 tcgtgacgtc gctcctcccg cgctcgcggc ggaagatggt gagcgacacg tgggatctga
     1141 tcgacgcgaa gctcggcgcc tacgtccggg gccagcttct gctgatcgcc ttcgtcgcga
     1201 cgatgctctc ggtcatcttc tgggcgatcg accttccgtt ctggctcctg ctcgccatct
     1261 tcgccggcgt cgtcgagatc atccccgtca tcgggccgcg cgccgccggc gcgctcgccg
     1321 tcggcgtcgg cctcaccgct tcgtggaagg tcgcgcttgc agcaggcatc gccgtcctcg
     1381 tggtgcgcct cttcgaggac tacgtggtca tcccgcgcgt gctcggggac gcggtcggcc
     1441 tcacgccgct caccgtcctc gtcgccgtgt cctcggtggc aatcctcttc ggcggattcg
     1501 ccgtcctcct ggccatcccg ctcgcggccg tgctcgcgac gctgatcgac gtcctcatcc
     1561 gccatcgcga tccctcacgg gaggacgttc caacggtgtt gttccctgcg aaagacgtcg
     1621 aggcgtaggg cgccgaactg cgctagccgc gctcgacctg ggagccgccc acgatcgcgt
     1681 cctcgcc
//
LOCUS       DASZOB010000270         5106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_66656, whole genome shotgun sequence.
ACCESSION   DASZOB010000270 DASZOB010000000
VERSION     DASZOB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66656"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VG709_04410"
     CDS             complement(<1..1338)
                     /locus_tag="VG709_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32351.1"
                     /translation="MDIPETRYTNVGDSQVAYQVFGEGPDLVYSTGFISHVDHRWDDP
                     GQRRFLSRLASFSRMIVFDRRGTGASDALPAGEGPTWEHWVDDLNAVLEAVGSERPSI
                     LANTDAGPMAMLFAATYPDRVSSLILTNTAARGSQAPDYPHGLAPEFQEPFLQTVEEG
                     WGKEDGALMRILAPEHAEDPEFVRWLARMQRASMTPRRAAEHWRIILGIDARHVLPLI
                     RVPTLVIGLSDVRSLPVANSEYLAEHIDGARLLVLRGTSVLGPYLSEPDRLVAAIEEM
                     VTGEHRPVMPDRILATVLLTDIVASTERASELGDRRWRELLDRHDRIAARHVDRFGGR
                     VVKGTGDGILAMFDGPARAVGAATEIRDELERSGITIRSGLHAGEVEVRGDDVGGIAV
                     HIAARVMALAPPGEILVSSTVKGLVAGSGIACEDRGTHTLKGVPDEWVLHAVPR"
     gene            1418..2185
                     /locus_tag="VG709_04415"
     CDS             1418..2185
                     /locus_tag="VG709_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWC32352.1"
                     /translation="MSEEQASRRESTRAQDRAGLTEDAKLLGTKQPSFLDSEPWRVLR
                     ITGEFVEGFEAMAAVPRAVSVFGSARVGPDATYYVAAREVGRALAEAGFAVITGGGPG
                     VMEAANRGCREAGGFSVGCTIELPVEQGANGYLDLQVHFQYFFVRKMMFVKYAEGFVV
                     FPGGFGTLDELFESLTLIQTGKALDFPVVLYGSDYWRGLLDWLRDPMLEQGKIAPDDM
                     SLLAVVDDPQAVVSFIEKVRAQRGSPTEPSPSKADAE"
     gene            complement(2189..2683)
                     /locus_tag="VG709_04420"
     CDS             complement(2189..2683)
                     /locus_tag="VG709_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWC32353.1"
                     /translation="MSPRAAWRLESIGFSQVFAYKSGKSDWLALGLPTEGHGTGVLKA
                     GDVARRDTPTCGLGEAMTEVTDRVRGGGWDVCVVVNDENVVLGLHRARHLDDERDRSV
                     EDRMIRAPSTFRPNVPAENLVRYMREHGLDDVPITTSDGKLIGLFRIEDAERALGWDE
                     RNGG"
     gene            complement(2687..2890)
                     /locus_tag="VG709_04425"
     CDS             complement(2687..2890)
                     /locus_tag="VG709_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWC32354.1"
                     /translation="MPKTVEVDEVGRLLEEGAQLVEVLSQEEYEDEHLPGAINIPLDA
                     IEREARERLDPSRPVIVYCWDDP"
     gene            complement(2905..3489)
                     /locus_tag="VG709_04430"
     CDS             complement(2905..3489)
                     /locus_tag="VG709_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWC32355.1"
                     /translation="MRADIETGASFPDYELTDHSGTRRRLFELQGDDPMILVLSRGHF
                     CPKDRRQLRNLVEFHPELKVGYTQLVTISTDNLVQTNELRDALGAQWPFLSDPKRKVQ
                     QDLDIKEYTDPHNDPMIPHTLVLEPGLRIYRIYNGYWYWGRPSIEDLRRDLGEMFMKI
                     RPDWDLAAPGLREAWESGDRDRFFPYGRRARRVA"
     gene            complement(3579..4265)
                     /locus_tag="VG709_04435"
     CDS             complement(3579..4265)
                     /locus_tag="VG709_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWC32356.1"
                     /translation="MGPRGSMLRHAAWIARSVGRGDLFPFTPADVAELAESIGVARLE
                     AGTRLLAPGGPVEWIGIIERGEVELSHRSGIRRVVLQILRDGDLLGDVPFFCRLPSPF
                     TARTLTDVMLIRLERVDVERLLATRPMLAQRFLYSLASRLERMQRRLLQLTGGDLRAQ
                     VAALLRDETEEGADLVRLPQATLAELLGARRPSVNRALKDLESRGVVRLGYRRIEIVD
                     ASALARVAGT"
     gene            4445..4957
                     /locus_tag="VG709_04440"
     CDS             4445..4957
                     /locus_tag="VG709_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32357.1"
                     /translation="MAGVAAGITGALVMAMFAMIAAATYHGTGFFTPMYHIASSVIAP
                     DTMMESAAQAQAGNLFYFEVWPAALGMALHLAVGAPFGALFPFVGRVVGVRGPAWIGL
                     GVLYGAVVLALMAFVGLPIAAAVFGGGDPIGSMPAMAGWWTFAIEHLMFGAVLGVWFA
                     RSRRRVEAAG"
BASE COUNT          743 a         1780 c         1770 g          813 t
ORIGIN      
        1 gcgtggtacc gcgtggagca cccactcgtc ggggacgccc ttcagcgtgt gggtcccgcg
       61 gtcctcgcac gcgatccccg accccgcgac caggcccttg accgtcgagc tgacgaggat
      121 ctcgcccggc ggggcgagcg ccatgaccct ggccgcgata tggacggcga tgccgccgac
      181 gtcgtcgccc cggacctcca cctctccggc gtggagcccg ctcctgatgg tgatgcctga
      241 tcgctcgagc tcgtcgcgga tctcggtggc agcaccgacc gcgcgggcgg gaccgtcgaa
      301 catggcgagg atcccgtctc cggtgccctt cacgacgcgt ccaccgaagc ggtcgacgtg
      361 acgcgccgct atgcggtcat ggcgatcgag gagctcccgc cagcgccgat cgccgagctc
      421 ggaggcccgc tcggtcgatg cgacgatgtc ggtgaggagc accgtcgcga ggatgcggtc
      481 gggcatcacg ggacggtgct ccccggtgac catctcctcg atcgccgcga cgagccggtc
      541 gggctccgac aggtacggcc ccagcacgct ggtgccgcgc aggacgagca gcctcgcgcc
      601 gtcgatgtgc tcggccaggt actcgctgtt cgcgaccggc aacgaccgaa cgtcgctgag
      661 cccgatcacg agcgtgggga cgcggatgag cggcaggacg tggcgtgcgt cgatcccaag
      721 gatgatcctc cagtgctctg ccgctcgacg tggcgtcatg ctcgcgcgct gcatacgcgc
      781 cagccacctc acgaactccg ggtcctcggc gtgctcgggc gcgaggatcc tcatcagcgc
      841 gccgtcctcc ttcccccacc cttcctcgac ggtctgcagg aacggctcct ggaactccgg
      901 cgcgagcccg tgcgggtagt ccggcgcctg cgacccgcgc gccgccgtgt tcgtgaggat
      961 gagagaggag acgcgatccg ggtaggtcgc ggcgaacagc atcgccatcg gccccgcgtc
     1021 ggtgttggcg aggatcgacg gccgctcgga gccgacggcc tccagcacgg cgtttaggtc
     1081 gtcgacccag tgctcccacg tcggcccctc gccggcgggc aacgcgtccg acgcgcccgt
     1141 ccctcggcgg tcgaacacga tcatccggct gaacgacgcg agacgcgaca ggaaacggcg
     1201 ctgtccgggg tcgtcccagc ggtgatcgac gtggctgatg aagcccgtcg agtacacgag
     1261 atccggtccc tcgccgaaga cctggtacgc gacctgcgag tcgcccacgt tcgtgtaccg
     1321 cgtctcgggg atatccacgc gacgaagact acgcggaggc ggtctcatcg agaaccgagg
     1381 ccgtgtcctc cggcgcgacg gtgggtatcg gcaggacgtg agcgaggagc aggcgtcgcg
     1441 ccgggagtcg acgcgcgccc aggatcgcgc ggggctgacc gaggacgcga agctgctcgg
     1501 gacgaagcag ccgtcgttcc tcgactccga gccgtggcgg gtgctgcgca tcaccggaga
     1561 gttcgtcgag gggttcgagg cgatggccgc ggttccccga gccgtgagcg tgttcggctc
     1621 ggctcgcgtc ggacccgacg ccacctacta cgtggccgcg cgagaggtcg gacgcgcact
     1681 ggccgaggcc ggcttcgcgg tgatcaccgg cggagggccc ggcgtcatgg aggccgccaa
     1741 ccgcggctgc cgcgaggccg gtgggttctc ggtcggttgc acgatcgagc tcccggtcga
     1801 gcaaggagcg aacggctacc tcgacctcca ggtgcacttc cagtacttct tcgttcgcaa
     1861 gatgatgttc gtgaagtacg cggaggggtt cgtcgtgttc ccgggagggt tcggaacgct
     1921 cgacgagctc ttcgagtcgc tgacgctcat ccaaaccggc aaggcgctcg acttccccgt
     1981 ggtcctgtac gggagcgact actggcgcgg cctcctcgac tggctccgcg atccgatgct
     2041 cgaacaggga aagatcgccc ccgacgacat gtcgctgctt gcggtcgtcg acgaccccca
     2101 agcggtcgtc tccttcatcg agaaggtccg ggcccagcgc ggctcgccga cggagccgtc
     2161 gccgtcgaag gccgacgcgg agtagctcct atccgccgtt cctctcgtcc cacccgagcg
     2221 cgcgctccgc gtcctcgatc cggaagagcc cgatcagctt gccgtccgat gtcgtgatcg
     2281 ggacgtcgtc gaggccgtgc tcgcgcatgt acctgacgag gttctcggcg gggacgttcg
     2341 gacggaacgt gctcggcgcg cggatcatgc ggtcctcgac cgagcggtca cgctcgtcgt
     2401 cgagatggcg agcgcggtgg agccccagga ccacgttctc gtcgttcacg acgacgcaga
     2461 cgtcccaccc accgccgcga acgcgatcgg tgacctcggt catcgcctcg ccgagtccgc
     2521 acgtcggcgt gtcgcggcgg gcgacgtcgc ccgccttgag gacccctgtc ccgtggccct
     2581 cggtcggcag gccgagcgcg agccagtccg acttccccga cttgtaggcg aagacctggc
     2641 tgaagccgat gctctcgagc cgccacgcgg cccgtgggct catgtctcag ggatcgtccc
     2701 agcagtagac gatcacgggg cgcgagggat ccaggcgctc gcgtgcctcg cgctcgatcg
     2761 catcgagcgg gatgttgatc gcgcctggga ggtgctcgtc ctcgtactcc tcttgggaca
     2821 gcacctcgac gagctgcgcc ccttcctcga ggagccgtcc gacctcgtcg acctcgacgg
     2881 tcttcggcac ccgtcctccc gccgctacgc gacgcgccga gcccggcggc cgtacgggaa
     2941 gaaccgatcg cggtcgccgc tctcccacgc ctcccggagc cccggcgccg cgaggtccca
     3001 gtccggccgg atcttcatga acatctctcc caggtcccgc cggagatcct cgatcgacgg
     3061 ccgcccccag taccagtacc cgttgtagat cctgtagatc ctgagccccg gctcgagcac
     3121 gagcgtgtgc gggatcatcg ggtcgttgtg cgggtccgtg tactccttga tgtcgaggtc
     3181 ctgctggacc ttgcgcttcg ggtccgagag gaacggccac tgcgctccga gcgcgtcgcg
     3241 gagctcgttc gtctgaacga ggttgtcggt cgagatcgtc acgagctgcg tgtagccgac
     3301 cttgagctca ggatggaact cgacgaggtt gcgcagctgc ctgcggtcct tcggacagaa
     3361 atgcccgcga gacaacacca ggatcatcgg atcgtcgccc tgcagctcga agaggcgccg
     3421 gcgcgttccg gaatggtcgg tgagctcgta gtcggggaac gaggcgccgg tctcgatgtc
     3481 ggctctcatg cgtcacctcc gtatccgttg gatctttctt accgcgcgag accaccgaag
     3541 cttccgccac tgtgccgacg cgaacagttc gcgccggctc aggtgccggc gacgcgggcg
     3601 agcgccgaag cgtcgacgat ctcgatccga cggtatccga ggcgcacgac gcctcgcgat
     3661 tcgaggtcct tgagcgcgcg gttcacgctc gggcggcggg caccgaggag ctcggcgagc
     3721 gtcgcctgcg ggagccggac gagatcggct ccttcttccg tctcgtcgcg gagcagcgcc
     3781 gccacctgcg cgcggagatc tccaccggtc agctgcagga gccggcgctg catccgttcg
     3841 agccgcgacg cgagcgaata gaggaaccgc tgcgcgagca tcggccgagt cgcgagcagc
     3901 cgctccacat cgaccctctc gagccggatc agcatcacgt cggtcagcgt ccgtgcggtg
     3961 aacggcgacg ggagccggca gaagaacggg acgtcgccga ggagatcccc gtctcggagg
     4021 atctggagga ccacgcgccg gatgccgctc ctatgcgaga gctcgacctc cccgcgctcg
     4081 atgatcccga tccactcgac cggtccgccc ggcgcgagca accgcgtgcc ggcctccagc
     4141 cgcgcgacac cgatgctctc cgcgagctcg gcgacgtctg cgggcgtgaa cgggaagaga
     4201 tcgccgcgac cgacgctcct cgcgatccac gccgcgtgcc gaagcatgct gcctcgtggc
     4261 cccatggcgg atcactcaac cacagcttcg ccggccctca cgcgaagagt gtcaccgaac
     4321 tgaccgttgc ggctgcctcg cagtgcgaaa cgcgttcatg ccttcgagga cgcgttgtcg
     4381 acgaagggag gcgacggtgg cgacgatatc cgaggcaacg accgtgcgct cgaggacggc
     4441 cctcttggca ggtgtcgcgg ccggcatcac cggcgcgctg gtgatggcga tgttcgcgat
     4501 gatcgcggcc gcaacgtacc acgggacggg gttcttcacg ccgatgtacc acatcgcctc
     4561 gagcgtgatc gcgcccgaca cgatgatgga gtccgcggcg caggcgcagg ccggcaacct
     4621 cttctacttc gaggtatggc ccgccgcgct cggcatggca ctccacctcg cggtcggtgc
     4681 gccgttcggc gcgctcttcc cgttcgtcgg ccgggtcgtc ggtgttcgcg gaccggcgtg
     4741 gatcgggctc ggggtgctct acggcgcggt cgtgctcgcc ctgatggcct tcgtcgggct
     4801 tccgatcgcg gccgcggtgt tcggcggcgg cgatccgatc gggagcatgc cagcgatggc
     4861 ggggtggtgg accttcgcga tcgagcacct catgttcggg gccgtcctcg gcgtgtggtt
     4921 cgcgcgctcg aggcggcgcg tagaagcggc gggatgaggg cgtcgcgcgt ggcggccgct
     4981 gcgctcatcg gggcgctggc tttgtcggcc tgcgcgcgcg acgccacgtc ggtcgctgac
     5041 gacgaggaga accgcgctcg tccgacgccg gcgcccagcc gcatcgacac cgacgacctc
     5101 gtgagg
//
LOCUS       DASZOB010000271         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_56254, whole genome shotgun sequence.
ACCESSION   DASZOB010000271 DASZOB010000000
VERSION     DASZOB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56254"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VG709_04445"
     CDS             complement(<1..449)
                     /locus_tag="VG709_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32358.1"
                     /translation="MTQTAYEAVPAVAPADPRASRGTIVMKFGGTSVADPEKVRRVAR
                     RLAAAKEDGARVVGVVSAMGHHTDELVSLAHDVSPRPKPRELDMLISVGERISCALVA
                     MAISDLGYDAISLTGSQAGIVTDTVHGKAKIVEVRARRIHQALDQDEV"
     gene            complement(515..>1585)
                     /locus_tag="VG709_04450"
     CDS             complement(515..>1585)
                     /locus_tag="VG709_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HWC32359.1"
                     /translation="PGGLTKLEAAIAAAAIALSVVVGVLHFTHASDVVTFILGAVALA
                     GIAWVVSFATEAVGERLGPAATGVLQSTLGNLPELFIVLFALSAGEVVVAQTSILGSI
                     FANALLVLGLVIIAGARRSEDGVMRFHARLPNDTATLLMLAVFMIVILGLSDTIGDRA
                     SEHQTTISTIGAVLLLLVYFAWLAVYLRGAPPPGAEAGARHAAPRVALPAAVALLAVS
                     GTAAALVSDWFVHALGPAVEQLGISRAFTGLVIVAIAGNAVENVAGVVLAWKGKSDLA
                     ISVGKNSVSQIAVFLFPVLVLLSLFFEERLTFVVGPVFIGALALTAIAVWQVPGDGEA
                     VEFEGGALVALYAILAVLVFFE"
BASE COUNT          268 a          524 c          603 g          190 t
ORIGIN      
        1 acctcgtcct ggtcgagcgc ctggtggatc cgccgggcgc gcacctccac gatcttcgcc
       61 ttgccgtgca cggtgtcggt cacgatgccc gcctgcgacc cggtcagcga gatggcgtcg
      121 tagccgaggt cggagatcgc catcgcgacg agcgcgcacg agatccgctc gccgaccgag
      181 atcagcatgt cgagctcgcg tggcttcggg cgcggcgaga catcgtgcgc gagcgagacg
      241 agctcgtccg tgtggtggcc catggccgag acgacgccga ccacgcgcgc gccgtcctcc
      301 ttcgccgccg cgaggcggcg cgcgacgcgg cggaccttct cggggtcggc gacggacgtc
      361 ccgccgaact tcatcacgat cgtgccgcgc gaggcgcgcg ggtcggccgg ggcgacggcc
      421 gggaccgctt cgtaggcggt ctgcgtcatg cggccgattg tagggacggg gccgaacggc
      481 tctcggaggg tcgcgggcgg gacaagtacc gcctctactc gaagaacacg agaaccgcga
      541 ggatcgcgta gagcgccacg agggcgccgc cctcgaactc gacagcctcg ccgtcgcccg
      601 gcacctgcca caccgcgatg gccgtgaggg cgagcgcgcc gatgaagacg gggccgacca
      661 cgaaggtgag ccgctcctcg aagaagagcg agaggaggac gagcaccggg aagagaaaca
      721 cggcgatctg gctgaccgag ttcttgccca ccgagatagc caggtcgctc ttccccttcc
      781 acgcgagcac gacgccggcg acgttctcga ctgcgttccc cgcgatcgcg acgatcacga
      841 gccccgtgaa cgcgcgcgag atcccgagct gctcgacggc gggtccgagc gcgtggacga
      901 accagtcgga gacaagggcg gcggccgtgc cgctgacggc gaggagcgct acggcggccg
      961 ggagagcgac gcgcggggcg gcgtgtcggg caccggcctc ggcgccggga ggcggcgctc
     1021 cgcgcaggta gacggcgagc caggcgaagt agacgaggag gagaagcacc gcgccgatcg
     1081 tcgagatggt cgtctggtgc tcgctcgccc ggtcaccgat cgtgtccgag agcccgagga
     1141 tgacgatcat gaagacggcg agcatgagca gcgtcgccgt gtcgttcggc aggcgcgcgt
     1201 ggaagcgcat gacgccgtcc tcggagcggc gtgcgccggc gatgatgacg agcccgagga
     1261 cgaggagcgc gttcgcgaag atcgagccga ggatcgacgt ctgcgcgacg acgacctcgc
     1321 ccgcggagag tgcgaagagg acgatgaaga gctcgggcag gttgccgagc gtggactgga
     1381 ggacgccggt cgcggccggt ccgagccgct cgccgacggc ctcggtcgcg aacgagacca
     1441 cccaggcgat ccctgcgagc gcgaccgcgc cgaggatgaa cgtgacgacg tccgaggcgt
     1501 gcgtgaagtg gaggacgccg acgacgacgc tgagagcgat tgccgcggcg gcgatcgccg
     1561 cctcgagctt cgtaaggcca cccgg
//
LOCUS       DASZOB010000272         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_26003, whole genome shotgun sequence.
ACCESSION   DASZOB010000272 DASZOB010000000
VERSION     DASZOB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26003"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VG709_04455"
     CDS             <1..1010
                     /locus_tag="VG709_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC32360.1"
                     /translation="APAMEPRAIEAEGLVMQYRKGPRAVDAIDLAVAPGEIYGFLGPN
                     GAGKSTTVHILTPLLPPTAGTARVAGYDVARQGPRVRALIGAALQEAALDPLLTGREH
                     LRLQATLQALPRAERKPRAEELLTRVGLAEAADRKVRGYSGGMKRRLDLALALVHSPR
                     VLFLDEPTTGLDVQSRTALWQEVVRLAKHEGVTVFLTTQYLEEADVLSDRIAIIDRGR
                     IVAEGTPAALKSQIGRPTVEAVPADASARDRAASVLGRFGEPVPCADGGVAVRLRSDE
                     TGLADVVRALDAEDVKVENLQLHQPSLDDVFLAKTGRSLEGAGRSAEELEDRKVAAEV
                     G"
     gene            1007..1738
                     /locus_tag="VG709_04460"
     CDS             1007..1738
                     /locus_tag="VG709_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC32361.1"
                     /translation="MSQALADQVLLLARRSVVRTARQPANVVFPLVFPLLLLAVNAGG
                     LDSATRLPGFPTSSFVAFALAVPFIQGALFATMNTGTDLARDIQTGFLNRLSLTPMRG
                     TALLAGQLGGVVAMGVVQALVYLSVGLAVGVRLESGPLGALGAFAALRTGSGEAVQGL
                     FPAFFVFLFISSMNMPRNLIEIDWFRWAATLNPVSYLIECVRSLIITGWDAEALVLGF
                     GIAAAIAAVALTVATMALQTRLTRT"
     gene            1735..>2229
                     /locus_tag="VG709_04465"
     CDS             1735..>2229
                     /locus_tag="VG709_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32362.1"
                     /translation="MRALAVAGAVAWRTLHTVFTNPSLFVPSLVFPLFFFIAFAGGLS
                     RVDEVPGFDFPLGYTAFQFVFVLLQSAAFGGVFTGFGIARDFESGFARRLLLAAPNRT
                     GIVFGYAAAALARWLVTAAVLTAVALVAWMQVGGDGVDIFGLYALAVVVNFAAVLWAA
                     GVAMR"
BASE COUNT          257 a          850 c          755 g          367 t
ORIGIN      
        1 aggctcctgc catggagccg cgtgcgatcg aggcggaggg cctcgtcatg cagtacagga
       61 aggggccgcg agccgtcgac gcgatcgacc tggctgtggc gccgggcgag atctacggct
      121 tcctcggccc caacggggcc ggcaagtcca cgaccgtcca catcctgacg ccgctcctcc
      181 cgccgacagc cggcacggcg cgcgtcgccg gctacgacgt cgcccggcag ggtccgcgtg
      241 tccgcgcgct catcggcgcg gcgctccagg aggcggcgct cgacccgctc ctcacgggac
      301 gcgagcacct gcgcctccag gcgacgctcc aggcgttgcc gcgcgcggag cggaagccgc
      361 gcgcggagga gctcctcacg cgcgtcggcc tggccgaggc tgcagaccgc aaggtgcgcg
      421 gctactccgg cgggatgaag cgccggctcg atctggcgct cgcgctcgtc cacagcccgc
      481 gcgtgctctt cctcgacgag ccgacgaccg ggctcgacgt ccagagccgg acggcgcttt
      541 ggcaggaggt ggtgcgcctg gccaagcacg agggcgtgac cgtcttcctg acgacgcagt
      601 acctcgagga ggcggacgtc ctctccgacc gaatcgcgat catcgaccgg gggcgcatcg
      661 tcgccgaagg aacccccgcc gccctcaagt cgcagatcgg ccggccgacc gtcgaagcgg
      721 tgcccgccga cgcctccgcg cgcgatcgcg ccgcctccgt gctcggccgc ttcggcgagc
      781 ccgtgccgtg cgcggacggc ggcgtcgccg tgcggctgcg ctcggacgag acgggcctcg
      841 ccgacgtcgt ccgcgcgctc gacgccgagg acgtcaaggt cgagaacctc cagctccacc
      901 agccgtcgct cgacgacgtc ttcctcgcca agacgggtcg ctccctcgag ggtgcaggcc
      961 ggtcggcaga ggagctcgag gaccggaagg tcgcagccga ggtcgggtga gccaggccct
     1021 cgccgaccaa gtcctgctcc tggcgcgccg ctcggtcgtg cgaacggctc gccagcccgc
     1081 gaacgtcgtc ttcccgctcg tctttccgct cctccttctc gccgtcaacg ccggaggtct
     1141 cgactccgcc acccggctgc ccggattccc cacgagctcg ttcgtcgcct tcgcgctcgc
     1201 cgttccgttc atccagggcg ccctgttcgc gacgatgaac acgggcacgg acctcgcgcg
     1261 cgacatccag accggcttcc tgaaccgcct gtcgctgacg ccgatgcgcg gcaccgctct
     1321 gctcgccggc cagctcggtg gcgtggtcgc gatgggcgtc gtccaggcgc tcgtctacct
     1381 gtcggtcggc ctcgcggtcg gcgtccggct cgagagtgga cccctcggcg cgctcggcgc
     1441 gttcgccgcg ctccgcaccg gctcggggga ggccgtgcag ggcctgtttc ccgccttctt
     1501 cgtcttcctc ttcatctcga gcatgaacat gccgcggaac ctgatcgaga tcgactggtt
     1561 ccgctgggcc gcgacgctca accccgtctc gtacctgatc gagtgtgtcc gctcgctcat
     1621 catcacgggc tgggacgcgg aggcgctcgt gctcgggttc ggcatcgcgg cggcgatcgc
     1681 ggccgtcgcg ctcacggtcg ccacgatggc cttgcagacg aggctgacgc gcacgtgagg
     1741 gcgctcgccg tagcgggtgc ggtcgcctgg cggacgctgc acacggtctt cacgaacccg
     1801 tccctcttcg tgccgtcgct cgtcttcccg ctcttcttct tcatcgcctt cgccggcggc
     1861 ctctctcgcg tcgacgaggt gccgggcttc gacttcccgc tcggatatac ggcgttccag
     1921 ttcgtcttcg tcctcctcca gtcggccgcg ttcgggggcg tcttcacggg cttcggcatc
     1981 gcccgcgact tcgagagcgg cttcgcgcga cggcttctcc tcgccgcgcc gaaccggacg
     2041 ggcatcgtct tcggctatgc ggccgccgcg ctcgcacgct ggctcgtcac cgcggcggtc
     2101 ttgaccgcgg tcgccctggt cgcctggatg caggtcggcg gcgacggcgt cgacatcttc
     2161 ggcctctacg cgctcgccgt cgtcgtcaac ttcgcggccg tcctgtgggc cgccggcgtc
     2221 gccatgcgg
//
LOCUS       DASZOB010000273         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_197885, whole genome shotgun sequence.
ACCESSION   DASZOB010000273 DASZOB010000000
VERSION     DASZOB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197885"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VG709_04470"
     CDS             <1..1299
                     /locus_tag="VG709_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC32363.1"
                     /translation="IPDDAPIEHVIFIVKENRSFDNYFATYPGADGATRGQTLEGDWV
                     RLRQAPDSYRHDITHSFSAGLYAINGGRMNGFNFIRFGDDLTGYTTYRNGNGIERYWA
                     YADRFVLADRFFTSMYGPTFPEHLYTVAADAFDITDNKASGADTEGSYCDDPHEFTPH
                     FRQDLTKREIDEIMRIEYSRMADHPERILDIAQYWEEIRTCVNIPVIQDQLEEKDISW
                     KYYAEINRWQNALQAVRHVREDPKMWRKVQDPSQFLEDIRRERLPQVTWVVPEEPYNE
                     HPGGEKSVCAGENWTVQQVNAVMKSKYWKSSVIVIVWDDFGGFYDHVVPPHDDIMGFG
                     IRTPALIISPYARQGDGPNGGYIASTTYEFSSVLRFIAELFALKPMTDRDAQANPLAG
                     ALDFESPPRYDKLMFPYRDDCPYGTELGWPDVRREVGLPN"
     gene            complement(1296..2459)
                     /locus_tag="VG709_04475"
     CDS             complement(1296..2459)
                     /locus_tag="VG709_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC32364.1"
                     /translation="MTGEPFTLPEELETFRKAVRAVCEDRLAPRAPEIDAEDEFPHDV
                     HRLFVENELMAVGYPEQHGGAGGGSLTFAVMIEEIARVSASASLIPLVSRLGAIPVML
                     AGSKDQKDQLFGGISRGDHQMSYALTEPGAGSDAGSMRTRYERTGGGFRLSGTKRFIT
                     NAGVSDRYTVFATRDPTERSRGISAFVVHADDAGFSVGKSEKKMGIRGSPTREVYLEG
                     VEIPAERLIGEEGQGFTYAMRTLDYSRPTIGAQALGIAQGAFDVAARYAGEREQFGKK
                     IGDFQGLQFLLADMAMEVEAARLLVYRAASACDAQDPKLTYLASVAKCYAGDAAMKVT
                     TDAVQVLGGYGYMREYGVERMMRDAKITQIYEGTNQIQRLVIARQVLRRLTGA"
     gene            complement(2470..>3435)
                     /locus_tag="VG709_04480"
     CDS             complement(2470..>3435)
                     /locus_tag="VG709_04480"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWC32365.1"
                     /translation="VPKEVPAITINKVCLSGLNAVAMADQLIRAGEIEIAVAGGMESM
                     SNAPYVLPSARWGARMGTAELVDSMIYDGLWSTFTDQHMGDSSDEINRELSISREAQD
                     DWSARSHLRAHEAWESGRLAEEVVAYEVPQRKRDPLVVSRDEGIRAETTTETLAKLPS
                     VFRKDGTITAGNASQISDGGAAMVVMSEERAQGIGAEPLAEVVAHGMSAERYAYLHTV
                     PALAMEAALKKAGKSASDLGLVEINEAFASVALNATKMLGMSEEIVNVNGGAVALGHP
                     IGMSGARLVLTLGYEMRRRGVELGGAALCGGGGQGDALIIRNPAA"
BASE COUNT          552 a         1234 c         1121 g          528 t
ORIGIN      
        1 atccccgacg acgcgccgat cgaacacgtc atcttcatcg tcaaggagaa ccgcagcttc
       61 gacaactact tcgcgacgta tcccggcgcc gacggcgcga cgcgcggcca gacgctcgag
      121 ggagactggg tccggctccg ccaggcgccg gactcgtacc gccacgacat cacgcacagc
      181 ttctccgcgg gcctctacgc gatcaacggc gggaggatga acggcttcaa cttcatccgc
      241 ttcggcgacg acctcaccgg ctacacgacg tatcggaacg gcaacgggat cgagcgctac
      301 tgggcgtacg ccgaccggtt cgtcctcgcc gaccgcttct tcacctcgat gtacgggccc
      361 accttccccg agcacctcta cacggtggcg gcggacgcgt tcgacatcac cgacaacaag
      421 gccagcggag cggacaccga gggcagctac tgcgacgacc cgcacgagtt cacgccgcac
      481 ttccgccagg acctcacgaa gcgcgagatc gacgagatca tgcggatcga gtacagcagg
      541 atggccgatc acccggagcg gatcctcgac atcgcgcagt actgggagga gatccgcacg
      601 tgcgtgaaca tcccggtgat ccaggaccag ctcgaggaga aggacatctc ctggaagtac
      661 tacgccgaga tcaaccggtg gcagaacgcg ctccaggccg tccgccacgt ccgcgaggac
      721 ccgaagatgt ggcggaaggt ccaggacccg tcgcagttcc tcgaggacat ccgccgcgag
      781 cggctcccgc aggtcacctg ggtcgtgccg gaggagccgt acaacgagca tcccggcgga
      841 gagaagagcg tctgcgccgg cgagaactgg acggtgcagc aggtcaacgc cgtcatgaag
      901 agcaagtact ggaagagcag cgtgatcgtg atcgtgtggg acgacttcgg cgggttctac
      961 gaccacgtcg tcccgccgca tgacgacatc atggggttcg gcatccggac cccggcgctc
     1021 atcatctcgc cgtacgcccg ccagggcgac ggaccgaacg gcggctacat cgccagcacg
     1081 acgtacgagt tctcgtcggt ccttcgcttc atcgcggagc tgttcgccct caagcccatg
     1141 accgatcgcg acgcacaggc gaacccgctc gcgggcgcgc tcgacttcga gtcgccgccc
     1201 cgctacgaca agctcatgtt cccgtaccgc gacgactgtc cgtacggcac cgagctgggc
     1261 tggccggacg tccgccgcga ggtcgggctg ccgaactagg cgccggtcag ccgacgcagg
     1321 acctggcgcg cgatcacgag ccgctggatc tggttcgtgc cctcgtagat ctgcgtgatc
     1381 ttcgcgtcgc gcatcatccg ctcgacgccg tactcgcgca tgtacccgta acctccgagg
     1441 acctgaacgg catcggtcgt gaccttcatc gcggcgtcgc ccgcgtagca cttcgcgacc
     1501 gatgccagat acgtgagctt ggggtcctgc gcgtcgcacg ccgacgccgc gcggtagacg
     1561 aggaggcgag cggcctcgac ctccatcgcc atgtcggcga gaaggaactg gagcccctgg
     1621 aagtccccga tcttcttccc gaactgctca cgctctcccg cgtaccgcgc cgcgacgtcg
     1681 aacgcgccct gcgcgatccc gagcgcctgg gcgccgatcg tcgggcgcga gtagtcgagg
     1741 gtgcgcatcg cgtacgtgaa cccctgcccc tcctcgccga tcagccgctc ggccgggatc
     1801 tcgacgccct ccaggtacac ctctcgggtc ggcgatccgc ggatgcccat cttcttctcg
     1861 gacttcccca cggagaaccc ggcgtcgtcg gcgtggacga cgaacgcgga gatgccgcgt
     1921 gagcgctctg tggggtcgcg cgtcgcgaag accgtgtacc ggtccgagac gccggcgttc
     1981 gtgatgaacc tcttggttcc cgacagccgg aagccgccac ccgtgcgctc gtagcgggtc
     2041 ctcatggacc ccgcgtccga gccggcgccg ggctcggtga gcgcgtacga catctggtgg
     2101 tcgccgcgcg agatacctcc gaagagctgg tccttctggt ccttcgagcc cgcgagcatc
     2161 acggggatcg cgccgagcct ggagacgagc gggatcagcg aggcgctcgc cgacacgcgc
     2221 gcgatctcct cgatcatcac cgcgaacgtc agcgagccgc cacccgcgcc gccatgctgc
     2281 tcgggatacc cgacggccat gagctcgttc tcgacgaaga ggcgatggac gtcgtgcggg
     2341 aactcgtcct ccgcgtcgat ctccggggcg cggggcgcga ggcggtcctc acatacggcg
     2401 cggacggcct tccggaaggt ctcgagctcc tccggaagcg tgaacggctc gccggtcacg
     2461 tgcgcgcgct caggccgcgg ggttgcggat gatcagcgcg tcgccctggc cgccgccgcc
     2521 gcagagggcc gcaccgccga gctcgacgcc ccgtcgacgc atctcgtacc cgagcgtcag
     2581 cacgagtcgc gcgccgctca tcccgatcgg gtggccgagg gcgacggcgc cgccgttcac
     2641 gttcacgatc tcctctgaca tgccgagcat cttggtcgcg ttgagcgcga ccgaggcgaa
     2701 cgcctcgttg atctcgacga gcccgaggtc cgaggcggac ttcccggcct tcttgagcgc
     2761 ggcctccatc gcgagcgccg gcaccgtgtg gaggtacgcg tagcgctcgg cgctcatgcc
     2821 gtgcgcgacg acctcggcga gcggctcggc gccgatgccc tgcgcgcgct cctcgctcat
     2881 cacgaccatc gcggcgccgc cgtcggagat ctgcgacgcg ttgccggccg tgatcgtgcc
     2941 gtccttgcgg aagaccgacg gcagcttggc cagcgtctcg gtcgtcgtct ccgcgcggat
     3001 cccctcgtcg cgagacacga cgagcgggtc tctcttccgc tgcgggacct cgtacgcgac
     3061 gacctcctcg gcgagccggc ccgactccca cgcctcgtgg gcgcggaggt gcgagcgggc
     3121 cgaccagtcg tcctgcgcct cgcgcgagat cgacagctcg cggttgatct cgtcggacga
     3181 gtcccccatg tgctggtcgg tgaacgtcga ccacagtccg tcgtagatca tcgagtcgac
     3241 gagctccgcg gtccccatgc gcgcgcccca ccgggcgctg ggcaggacgt acggcgcgtt
     3301 cgacatggat tccatgccgc cggccaccgc gatctcgatc tcgccggccc ggatcagctg
     3361 gtccgccatg gccaccgcgt tcaggccgga gaggcacacc ttgttgatgg tgatcgccgg
     3421 gacctccttc gggac
//
LOCUS       DASZOB010000274         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_107138, whole genome shotgun sequence.
ACCESSION   DASZOB010000274 DASZOB010000000
VERSION     DASZOB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107138"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VG709_04485"
     CDS             <1..1246
                     /locus_tag="VG709_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HWC32366.1"
                     /translation="PGVIPSHARQRLFLPSPLDGSPRPLTEKLLERPVLLDRIRSLIA
                     APDRAHLVPYNTTELERELAVQLGIPMYGADPKHFPLGTKTGCRRLFAEEGLPYPRGA
                     EDLHTVEDVVDAIASLRADAPELRSVLIKLNEGVSGEGNAEVSLDGVPRPGDATERDA
                     IAERVRAMRFELDEVTLDDYVTKLKERGGVVEERIAGTEFRSPSVQLRVTPLGELELL
                     STHDQLLGGRGGQTYLGARFPADPDYASTITAEAAKVGRRLATEGVIGRFAIDFVVVR
                     DEGGPWRPYAIELNLRKGGTTHPFLTLQFLTDGTYDAATAMFTAPSGRKKFFVASDQL
                     KKERYHGFTPDDLFDIAVRRGLHFDQSRQTGVVFHMISGLPEHGRVGLTAIGDSPEQA
                     DEFYALTENVLDEEAARAFPGV"
     gene            1270..1887
                     /locus_tag="VG709_04490"
     CDS             1270..1887
                     /locus_tag="VG709_04490"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWC32367.1"
                     /translation="MKIDAIKAARKRITKRPEERREDILEAAVRVFANKGISRTTVSD
                     ITDAAGVAKGTFYLYFASKEHLLGALKERFVTEILNRATTFYSRVGRAEWWTLVDETI
                     ETFVDFHLENKDLIQIIVQEGITPDTSDLFAECERKLDVMFSAGIQAGIEAGVFKVGD
                     PELAARMLHHALDGTLNHAVLYKEEIDRDRYVAAAKEITHKILAP"
     gene            complement(1898..2047)
                     /locus_tag="VG709_04495"
     CDS             complement(1898..2047)
                     /locus_tag="VG709_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HWC32368.1"
                     /translation="MQRWEYATVPLIEHATQEILNNWGDDGWELVTVLQGPQGIVAYL
                     KRPKG"
     gene            complement(2049..2489)
                     /locus_tag="VG709_04500"
     CDS             complement(2049..2489)
                     /locus_tag="VG709_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWC32369.1"
                     /translation="MDVSPYAPAYVPGDEDLRSIVRDAKTIAVVGLSSDPMRASFGVA
                     EELQQRGYRVIPVNPNEAEVLGEKAYPSLREVEGDVDLVDVFRRPEHTPAVAEDAVAI
                     GAKALWLQQGIVNDDARRIAEDGGLKVVMGICIRDALALLDAED"
     gene            complement(2480..3049)
                     /locus_tag="VG709_04505"
     CDS             complement(2480..3049)
                     /locus_tag="VG709_04505"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWC32370.1"
                     /translation="MASIGEVLSRALTPSRPDPPLPEGIPAAVVVPLLDLRDPAVVFT
                     KRTDLVSYHKGEISFPGGGRHPEDPDLLATALRETEEELGIPREAIEIAGTLPPTPTV
                     ATRYVIVPFVGILSERPEMIPSPVEIAEVIELRLERLIDVERAVEGVGPDGIARSWFA
                     YELDGYLVWGATGRILHGLIDVLRKEGWT"
     gene            complement(3099..>4464)
                     /locus_tag="VG709_04510"
     CDS             complement(3099..>4464)
                     /locus_tag="VG709_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32371.1"
                     /translation="PKEVLEFDAIGRIDDTEPADPTGAKGNGTVVTAVNVHYAVWTPA
                     GTPLAGPDRIEDLFAGMVPSRTIVFDPKVVYDPKQGRFYLVIVAYTDSTPPDSHILIA
                     SFSDDEASTPLLWCKRRLPGDAVPGDGNLFADYPGVGFDDRRLYVATNQFNQRDRFRY
                     AQVLAINRADLGSGCTGNVRFRKFWDLREPSGRRAFTVQPAVSIGSAAARAGTGISFD
                     WRCFPCRGRKVTIWRWAARDGALRQRAKSLDVGVARVAPLGRQRGGGGLDARWDTGDL
                     RLVTSFFDTDRQRLYGGHSVLRNLGRSSSPNESVARWYELYAPGRLKLAGVRRKGIVG
                     TPNKDSAWPAIAANGNGRVFLSYSRAAPDEFLSAWGAVIQPKGTGRELIRLRRGEARH
                     EAQAATPGSSGVERWGDYNAITRDPVAPLRMWSINQYAKDDRDRSNGVRSELWQQVVN
                     ALRA"
BASE COUNT          701 a         1579 c         1517 g          667 t
ORIGIN      
        1 ccccggcgtg atccccagcc atgcgcggca gcggttgttc ctcccgtcgc cgctcgacgg
       61 ctcaccgagg ccgctcaccg agaagctgct ggagcgacca gtgctgctcg accgcatccg
      121 ttcgttgatc gccgctcccg accgcgcgca cctcgtgccg tacaacacca cggagctgga
      181 gcgtgagctc gcggtgcagc tcggtatccc gatgtacgga gccgatccga agcacttccc
      241 gctgggaacg aagacgggat gccggcgcct cttcgcggaa gaggggctgc cgtatccgcg
      301 cggcgccgag gatctccaca ccgtcgagga tgtcgtcgac gcgatcgcga gcctccgcgc
      361 cgacgcgccg gagctgcggt cggtcctcat caagctcaac gaaggagtgt ccggcgaggg
      421 caacgccgaa gtctcactcg acggcgttcc acgccccggc gacgcgaccg agcgcgacgc
      481 gatcgcggaa cgcgtgcgcg cgatgcgctt cgagctcgac gaggtgacgc tcgacgacta
      541 tgtgacgaag ctcaaggagc gaggcggcgt cgtcgaggag cgcatcgccg gcaccgagtt
      601 ccggagcccg agcgtgcagc tccgcgtgac gccgctcggc gagctcgagc tcctgtcgac
      661 ccacgaccag ctgctcggcg gtcgtggggg ccagacgtac ctcggggcgc gcttccccgc
      721 cgatcccgat tacgcgtcga cgatcaccgc cgaagccgcg aaggtcgggc gacggctcgc
      781 gacggaagga gtcatcggcc ggttcgcgat cgacttcgtc gtcgtgcgcg acgagggcgg
      841 gccgtggcgt ccgtacgcga tcgagctcaa cctccggaag ggaggaacga cgcacccgtt
      901 cctcacgctg cagttcctca cggacgggac gtacgacgct gcgaccgcga tgttcacggc
      961 gccgagcgga cgcaagaagt tcttcgtcgc gagcgatcag ctcaagaagg agaggtacca
     1021 cgggttcaca cccgacgacc tgttcgacat cgcggtgcgc cgcgggctgc acttcgacca
     1081 gtcgcgacag accggcgtcg tgttccacat gatcagcggg ctccccgaac acggacgcgt
     1141 cggcctaacc gcgatcggcg actcgccgga acaagccgac gagttctacg cgcttaccga
     1201 gaacgtcctc gacgaggagg ctgcgcgcgc gttcccgggg gtatagttcc ttctgaccgg
     1261 gagtcagtct tgaagatcga cgccatcaag gccgcgcgca agcgcatcac gaagcgaccg
     1321 gaggagcgcc gcgaggacat cctcgaggcc gccgttcgcg ttttcgcgaa caagggcatc
     1381 tcgcggacga cggtctccga catcaccgac gcggccggcg tcgcgaaggg aaccttctac
     1441 ctctacttcg cgagcaagga gcacctgctc ggcgcgctca aggagcgctt cgtcaccgag
     1501 atcctcaacc gggcgacgac gttctactcg cgcgtcggtc gcgcagagtg gtggacgctc
     1561 gtcgacgaga ccatcgagac gttcgtcgac ttccacctcg agaacaagga cctgatccag
     1621 atcatcgtcc aggagggcat cacgcccgac acgtccgacc tcttcgcgga gtgcgagcgg
     1681 aagctcgacg tgatgttctc cgcagggatc caggccggca tcgaggcggg cgtgttcaag
     1741 gtcggcgacc ccgagctggc ggcgcgcatg ctccaccacg cgctcgacgg gacgctgaac
     1801 cacgcggtgc tctacaagga ggagatcgac cgcgaccggt acgtcgcggc cgcgaaggag
     1861 atcacgcaca agatcctcgc gccgtagcgc gcggatctca gcccttcggg cgcttcaggt
     1921 aggcgacgat cccctgcggg ccctgaagca ccgtcacgag ctcccacccg tcgtcgcccc
     1981 agttgttgag aatctcctgc gtcgcgtgct cgatcagcgg caccgtcgcg tactcccagc
     2041 gctgcacgtc agtcctccgc gtcgagcagc gcgagcgcgt ccctgatgca gatccccatc
     2101 acgaccttca gtcctccgtc ctcggcgatg cgtcgcgcgt cgtcgttcac gatgccctgc
     2161 tgcagccaca gcgccttggc gccgatcgcg acggcgtcct ccgcaacggc cggcgtgtgc
     2221 tccgggcgac ggaagacgtc gacgaggtcg acgtccccct cgacctcgcg gagcgacggg
     2281 tacgccttct cgccgagaac ctcggcctcg ttcgggttca ccgggatcac gcggtagccg
     2341 cgctgctgga gctcctcggc gacgccgaac gaggcgcgca tcggatcgct cgagaggccg
     2401 acgaccgcga tcgtcttcgc gtcgcgcacg atcgagcgca gatcctcgtc gccgggcacg
     2461 tacgcaggcg cgtacgggct cacgtccatc cctccttcct caatacgtcg atcaggccgt
     2521 ggaggatacg tccggtggct ccccacacga ggtacccgtc aagctcgtac gcgaaccacg
     2581 agcgcgcgat cccgtcgggc ccgacgccct cgacggctcg ctcgacgtcg atcagccgct
     2641 caagccgaag ctcgatgacc tcggcgatct cgacggggct cgggatcatc tcgggccgct
     2701 ccgagaggat ccctacgaac ggcacgatca cgtagcgcgt cgccaccgtc ggcgtcgggg
     2761 gaagcgtccc ggcgatctcg atcgcctcgc gcgggatacc gagctcctcc tcggtctcgc
     2821 gaagcgccgt cgcgaggaga tcggggtcct ccgggtggcg gccgccgccc gggaacgaga
     2881 tctcgccctt gtggtagctg acgagatcgg tgcgcttggt gaagacgacg gcggggtccc
     2941 gaagatcgag gagcggcacg acgacggcgg cggggatgcc ctccgggagc ggcgggtcag
     3001 ggcgcgaggg tgtgagcgct cgcgacagga cctcgccgat cgacgccacg ggcgtcatcg
     3061 taatgagcgg tcgcggagcc gctccggcgc ggagccgctc aggcgcggag cgcgttgacg
     3121 acctgctgcc agagctcgct gcgcactccg ttgctcctat cgcggtcgtc cttcgcgtac
     3181 tggttgatgc tccacatccg gagcggcgcg acggggtcgc gcgtgatcgc gttgtagtcg
     3241 ccccaccgct cgacgcctga cgaccccggt gtcgccgcct gggcttcgtg acgggcctcg
     3301 ccgcggcgaa gacggatgag ctcgcggccg gtccccttcg gctggatgac ggccccccat
     3361 gccgagagga actcgtcggg cgccgcgcgg ctgtagctca ggaagacgcg gccgttgccg
     3421 ttcgcagcga tcgcgggcca ggcggagtcc ttgttcggcg tcccgacgat gcccttgcgc
     3481 cggacgcccg cgagcttcag ccgcccgggt gcgtagagct cgtaccagcg agcgaccgac
     3541 tcgttgggcg acgagctgcg tccgaggttc ctcaggacgg aatgcccgcc gtacagccgc
     3601 tggcgatcgg tatcgaagaa cgacgtgacg agccgcaggt cgccggtgtc ccaccgcgcg
     3661 tcgagccctc cgccgcctcg ctgccgcccg agtggcgcca ccctcgccac gccgacgtcc
     3721 aggctcttcg cgcgctggcg cagcgcgccg tcgcgcgccg cccaccgcca gatcgtgacc
     3781 ttccgccccc ggcacgggaa gcaacgccag tcgaagctga tcccggtccc ggcgcgcgcg
     3841 gcggcgctgc cgatgctcac cgccggctga accgtgaagg cgcgtcgccc gctcggctcg
     3901 cggaggtccc agaacttccg gaagcgaacg ttgccggtgc acccgctccc gaggtccgcg
     3961 cggttgatcg ccaggacctg cgcgtagcgg aaccggtcgc gctggttgaa ctggttcgtc
     4021 gcgacgtaga gcctgcggtc gtcgaacccg acgccggggt agtcggcgaa caggttcccg
     4081 tcgccgggca cggcgtcacc cggcagccgg cgcttgcacc acaggagcgg cgtcgaggcc
     4141 tcgtcgtcgg agaacgacgc gatgaggatg tgggagtccg gcggcgtgct gtccgtgtac
     4201 gcgacgatca cgaggtagaa gcggccttgc ttggggtcgt acacgacctt cgggtcgaac
     4261 acgatcgtcc gcgagggcac catgcccgcg aagagatcct cgatccggtc cgggcccgcg
     4321 agcggcgtcc cggccggcgt ccagaccgcg tagtggacgt tcaccgcggt cacgacggtc
     4381 ccattcccct tcgcgccggt tggatccgcg ggctcggtgt catcgatccg gccgatcgcg
     4441 tcgaactcga ggacctcctt gggc
//
LOCUS       DASZOB010000275         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_26028, whole genome shotgun sequence.
ACCESSION   DASZOB010000275 DASZOB010000000
VERSION     DASZOB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26028"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /locus_tag="VG709_04515"
     CDS             <1..82
                     /locus_tag="VG709_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32372.1"
                     /translation="RQAVTAAGMGCKAAIDAERYLESLGH"
     gene            88..786
                     /locus_tag="VG709_04520"
     CDS             88..786
                     /locus_tag="VG709_04520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWC32373.1"
                     /translation="MRRVAAGAVLLAAMLSPAAPAPAAAPGFVAEPAYYRPALLDGLV
                     FPVARSNFFSLVEFDNGWNAPRFRLVDGTWRLVGVHKGIDIGAEQDTPILSVTPGVVE
                     NVGWTFYSGLRVGVRGPDGRYYFYAHLDDPAPGIAEGARVAAGDVLGTVGNTGYGPPG
                     HEDEFPPHLHFGIEGPDGWVNPYPDLVRLYRHAVRTARQAEDRLARLASAGDEAAWRA
                     EADGLYTTFGIPLP"
     gene            846..1187
                     /gene="trxA"
                     /locus_tag="VG709_04525"
     CDS             846..1187
                     /gene="trxA"
                     /locus_tag="VG709_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWC32374.1"
                     /translation="MTDTNTNEIPHVGDADFDTQVLGAERPVLVDFWADWCVPCHMVS
                     PVVEEIARENAGALKVAKLNVDDNPRTAQKYGVMSIPTLMLFKDGEERARVIGARGKD
                     QILKEIQPHFA"
     gene            1272..>1708
                     /locus_tag="VG709_04530"
     CDS             1272..>1708
                     /locus_tag="VG709_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HWC32375.1"
                     /translation="MRAIRHGSRGDGVRDVQARLTALGLRIDPDETGRFGEGTERAVR
                     EFQQRRGLVVDGIVGVGTWRELVDAGYTLGDRTLYLHQPYLRGDDVRVLQARLDALGF
                     DPGRVDGILGDRTDRAIRDFQRNVGLPPDGIGGATTLRALDRLR"
BASE COUNT          228 a          622 c          631 g          227 t
ORIGIN      
        1 ccgccaggcg gtcaccgcgg ccgggatggg gtgcaaggcg gcgatcgacg ccgagcgcta
       61 cctcgagtcg ctgggtcact gatgcgcgtg cggcgcgtcg cggccggagc cgtgctcctc
      121 gcggcgatgc tctcccccgc cgcgccggcc ccggcggcgg cgcccgggtt cgtcgccgag
      181 cccgcgtact accggcccgc gctcctcgac ggcctcgtct tcccggtcgc gcggtcgaac
      241 ttcttctccc tcgtcgagtt cgataacggc tggaacgcgc cgcggttccg gctcgtggac
      301 gggacgtggc ggctcgtcgg cgtccacaag gggatcgaca tcggcgccga gcaggacacc
      361 ccgatcctgt cggtgacccc gggggtcgtg gagaacgtcg gatggacctt ctactccggg
      421 ctgcgcgtcg gggttcgcgg ccccgacggc cgctactact tctacgccca cctcgacgac
      481 ccggcgccgg ggatcgcgga gggcgcccgc gtcgcggcgg gcgacgtcct cgggacggtc
      541 gggaacaccg gctacgggcc gcccgggcac gaggacgagt tcccgccgca cctgcacttc
      601 gggatcgagg ggcccgacgg gtgggtaaac ccctatccgg acctggtgcg gctctaccgg
      661 cacgcggtcc ggacggcgag gcaggcggag gaccggctcg cgcggctcgc gtccgccgga
      721 gacgaggcgg cgtggcgcgc cgaggccgac ggcctctaca cgacgttcgg aataccgctg
      781 ccgtagggag cgttccacgg ttcacgcggt cggcgccccc gcccgccccg acggaggacg
      841 acacgatgac tgacaccaac acgaacgaga tcccccacgt cggcgacgcc gacttcgaca
      901 cgcaggtcct cggcgcggag cggccggtcc tggtcgactt ctgggccgac tggtgcgtcc
      961 cgtgccacat ggtgtcgccg gtcgtcgagg agatcgctcg cgagaacgcg ggcgcgctga
     1021 aggtcgcgaa gctgaacgtc gacgacaacc cgcgcaccgc gcagaagtac ggcgtgatga
     1081 gcatccccac gctgatgctc ttcaaggacg gcgaggagcg cgcccgggtc atcggcgcgc
     1141 gcggcaagga ccagatcctc aaggagatcc agccgcactt cgcgtgacgc cccgcggcga
     1201 gcgccgcggc cggagcgacc ctcgccgctc gggggtttgt caccccgttc gactcgcgtg
     1261 agacttcgtc gatgcgagcg atccgccacg gttcgcgagg cgacggcgtt cgcgacgtcc
     1321 aggcgcgcct gacggccctc gggctccgga tcgaccccga cgagaccggc cggttcggcg
     1381 aggggaccga gcgcgcggtg cgcgagttcc agcagcgccg cgggctggtc gtcgacggca
     1441 tcgtcggggt gggcacgtgg cgcgagctcg tcgacgccgg ctacacgctc ggcgaccgga
     1501 cgctctacct gcaccagccg tatctccgcg gcgacgacgt ccgtgtgctc caggcgcggc
     1561 tcgacgcgct cgggttcgat ccggggcggg tcgacggcat cctgggtgac cggacggacc
     1621 gcgcgatccg cgacttccag cgcaacgtcg gcctcccgcc cgacgggatc ggcggggcga
     1681 ccacgttgcg ggcgctcgac cggctgcg
//
LOCUS       DASZOB010000276         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_197922, whole genome shotgun sequence.
ACCESSION   DASZOB010000276 DASZOB010000000
VERSION     DASZOB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197922"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..418)
                     /locus_tag="VG709_04535"
     CDS             complement(179..418)
                     /locus_tag="VG709_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HWC32376.1"
                     /translation="MIQLLLRAVVPIALIALFVYALVDAVRVPDDSMYQAGNKLIWVI
                     LIVLVPVLIGPAIYLIVGKPIRERPNRRPPPDDVI"
     gene            complement(415..1242)
                     /locus_tag="VG709_04540"
     CDS             complement(415..1242)
                     /locus_tag="VG709_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC32377.1"
                     /translation="MELRDAAVLLTGGSRGIGPYIARALLDRGARVTLAARSAEDLER
                     TKESLGADGVTTVAADLTDDDGRRSMVEGAERAFGPIDVLVNNAAWEQVLAFAHQTEE
                     DIVATTRLNLEAPLLLTRMLLPGMLERRRGHVVNISSVAGKAAVPWNTVYSATKHALV
                     GFSLSLRSELRGSGVGVSVICPGYVKDAGLYAEHRLVYEPPRSGTGTTPHKVAEAVVR
                     AVERDLPEIVVSGFLPKLSDVTLAISPTLFDRAARRSGGWLPMKREAEARAKQRGLR"
     gene            1285..2664
                     /locus_tag="VG709_04545"
     CDS             1285..2664
                     /locus_tag="VG709_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HWC32378.1"
                     /translation="MAERTQFLLEPEDLPTAWYNIMPDIAGAGLQPMPPLHPGTKEPV
                     GPQDLAPLFPETLILQEVSTDQWIDIPGPVLDVYRLWRPTPLFRATRLERALGTPARI
                     YFKYEGTSPAGSHKPNTAVAQAYYNKEAGIRRISTETGAGQWGSALAMACKFFDLECL
                     VFMVKASYDQKPYRRIFMETFGAEVRPSPTSTTESGKNVLEGDPDSPGSLGIAISEAI
                     EVAAQSQDTNYSLGSVLNHVLMHQTVIGLETKRQMEMAGDEPDVVIGCVGGGSNYAGL
                     AYPFMAERLQGKKQTRFVAAEPAACPTLTKGKFAYDFGDTGEMTPLLPMFTLGHTFVP
                     TPVHAGGLRYHGDAPSLSLLVREGHMDAKAYSQNQVFEAATRFAQTQGIIPGPEPAHA
                     VKAVIEEADAAREAGEERVILFNLSGHGFFDMQAYDDYLNERLPEVEYRDEQLEQGLQ
                     SVPEVPVGG"
     gene            2667..3113
                     /locus_tag="VG709_04550"
     CDS             2667..3113
                     /locus_tag="VG709_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32379.1"
                     /translation="MDEEAFRPGTREEELATAQTVLMAGGPFKNKNGVATLTNDRFVF
                     ADQRFSAQAAGAAGGMLAGAVAGALEKRRSKGKPPMIDVPLTDVTRVAHEKKMTVRDI
                     LVIEAKGEEHRFAQGFKALRPLLRRALPERHGRNVVDDGEGFRVSG"
     gene            complement(3129..3629)
                     /locus_tag="VG709_04555"
     CDS             complement(3129..3629)
                     /locus_tag="VG709_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32380.1"
                     /translation="MRRGPWIIAGGIVVAALIVAGTLWVLDLRREPAGVTGPSPSPSP
                     ETPTVSPSPSPSPTQSPSPSPSEAPLRQARVVAATFFSAWVTNDRPLALTVGTEDAVH
                     MAFLVPVRSRDSTRLQRCSLNEDLGAGSYQCVVVDRASDIPVMSMVARPVDGEMKVAE
                     VIFHAD"
     gene            3694..>4671
                     /locus_tag="VG709_04560"
     CDS             3694..>4671
                     /locus_tag="VG709_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC32381.1"
                     /translation="MGSDDHRVRFRPLDPLDVDALLSDEERAVRDTTRDWVKSRVLPE
                     IAEWFERGEIPSRELAKELGGMGLLGMHLSGYGTAGASAVMYGLAVHELEYGDSGVRS
                     LVSVQGSLAMFPIWKFGSDEQKDEWLPRMASGDAIGCFGLTEPDIGSDPASMRTSARR
                     DGDDWVLSGTKMWITNGGIADVAVVWARTDDGVRGFVVPTEAPGFSTNEIERKISLRA
                     SVTSELVLDDVRLPSSAMLPGVRGMRGPLSCLNEARYGIVWGAMGAARACYDAALEYA
                     TTRHQFGKPIGGFQLTQRKLVDMAVEIGKGMLLALHLGRMKDEGRAAPEH"
BASE COUNT          765 a         1563 c         1695 g          648 t
ORIGIN      
        1 atcccgaaga tcgcgaagaa gccgccgccg tcgtctacct gtgcgagcat gccgcgacag
       61 taggagccgt ccccagggct gtcaatcctc cgtgccgctc ctgtgccgtg gccgtcccat
      121 cccgtcgaga agatgcgaac cggcctggtg tgccgtcaat ggagcggcgc gcgatgcatc
      181 agatgacatc atcgggcggc gggcggcgat tcgggcgctc gcgtatcggc ttcccgacga
      241 tgagatagat cgccggaccg atcagcacgg ggacgagcac gatcaagatc acccagatca
      301 gcttgttccc ggcctggtac atcgagtcgt ccggcacacg gaccgcgtcg acgagcgcgt
      361 acacgaagag agcgatcaga gcgatcggaa ccaccgcacg aaggaggagc tggatcatcg
      421 gagcccgcgc tgcttcgcgc gcgcctccgc ctcgcgcttc atcgggagcc agccgccaga
      481 gcggcgggct gcgcggtcga agagggttgg cgagatcgcg agcgtgacgt ccgacagttt
      541 cggaaggaaa cccgagacga cgatctcggg aagatccctc tcgaccgcgc ggacgacagc
      601 ctcggcgacc ttgtgtgggg tcgtgcccgt cccggaccgc ggcggctcgt acacgagccg
      661 gtgctcggcg tagagacccg cgtccttgac gtagcccggg cagatcacgg agacgccgac
      721 gccgctgcca cggagctccg accgtagcga gagtgagaac ccgacgagcg cgtgcttcgt
      781 cgccgagtac acggtgttcc aggggaccgc ggccttcccc gcgaccgagg agatgttgac
      841 gacgtgcccc cggcggcgct cgagcatccc gggcagcagc atcctcgtca gcaggagcgg
      901 cgcctcgagg ttgaggcgcg tcgtcgcgac gatgtcctcc tccgtctgat gggcgaacgc
      961 gagcacctgc tcccacgccg cgttgttgac gaggacgtcg atgggcccga acgcgcgctc
     1021 ggcgccttcg accatcgagc ggcggccgtc gtcatcggtg aggtcggcgg ccacggtcgt
     1081 gaccccgtcg gcgccgagcg actccttcgt ccgctcgaga tcctccgcgg agcgagccgc
     1141 gagcgtcacg cgggcacccc ggtcgagcag cgcgcgggcg atgtacggcc cgatcccgcg
     1201 cgaccctccg gtcaggagaa ccgcggcgtc tcggagctcc acgggccgga ctatactcgc
     1261 gctccccagc ggaaggaggt cgagatggca gaacgaacgc agttccttct cgagccggag
     1321 gacctaccga ccgcctggta caacatcatg ccggacatcg ccggcgccgg gttgcagccg
     1381 atgccgccgc tgcatcccgg gacgaaggag ccggtcgggc cgcaggacct cgcgccgctc
     1441 ttcccggaga ccctgatcct gcaggaggtc tcgaccgacc agtggatcga catcccgggg
     1501 ccggtcctcg acgtctaccg cctgtggagg ccgacgccgc tgttccgcgc gacgaggctc
     1561 gagcgggcgc tcggcacgcc ggcgcggatc tacttcaagt acgaagggac gtcgccggcg
     1621 gggtcccaca agccgaatac cgccgtggcg caggcgtact acaacaagga ggccggcatc
     1681 cggcggatca gcaccgagac cggcgccgga cagtggggct ccgcgctcgc gatggcatgc
     1741 aagttcttcg acctggagtg cctcgtgttc atggtgaagg cgtcgtacga ccagaagccg
     1801 taccggcgca tcttcatgga gacgttcggc gcggaggtcc ggccgtcgcc gacgtccacg
     1861 accgagtcgg ggaagaacgt gctcgagggc gatcccgact cgccgggctc gctcggcatc
     1921 gcgatcagcg aggccatcga ggtcgccgcg cagagccagg acacgaacta ctcgctcggc
     1981 agcgtcctca accacgtcct gatgcaccag acggtgatcg gtctcgagac gaagcggcag
     2041 atggagatgg cgggggacga gcccgacgtg gtgatcggat gcgtcggcgg tggctcgaac
     2101 tacgcgggcc tggcgtaccc gttcatggcg gagcgactcc agggcaagaa gcagacgcgg
     2161 ttcgtcgcgg cggagcccgc ggcgtgcccg acgctcacca aggggaagtt cgcgtacgac
     2221 ttcggcgaca ccggagagat gacgccgctg ctgccgatgt tcacgctcgg gcacaccttc
     2281 gtcccgacgc cggtccacgc cggcgggctt cggtaccacg gcgacgcgcc gtcgctgtcg
     2341 ctgctcgtcc gcgaggggca catggacgcg aaggcgtact cgcagaacca ggtgttcgag
     2401 gccgcgacgc ggttcgcgca gacgcagggc atcatccccg ggcccgagcc ggcgcacgcc
     2461 gtgaaggcgg tgatcgagga ggccgacgcg gcgcgcgagg cgggcgagga gcgcgtgatc
     2521 ctcttcaacc tctcgggcca cggcttcttc gacatgcagg catacgacga ctacctgaac
     2581 gaacggctgc cagaggtgga gtaccgcgac gaacagctcg agcagggcct tcagagcgtc
     2641 cccgaggtcc cggtcggagg gtgacgatgg acgaggaagc gtttaggccc ggcacgcggg
     2701 aggaggagct cgcgaccgcg cagaccgtcc tcatggcggg cgggccgttc aagaacaaga
     2761 acggcgtggc gacgctcacg aacgaccggt tcgtgttcgc cgatcagcgc ttcagcgcgc
     2821 aggcggccgg agcggctggc gggatgctgg ccggcgccgt cgccggtgcg ctcgagaagc
     2881 ggcggagcaa agggaagccg ccgatgatcg acgtccccct gaccgacgtg acgcgcgtcg
     2941 cgcacgagaa gaagatgacc gttcgagaca tcctcgtgat cgaggcgaag ggggaggagc
     3001 accggttcgc acagggcttc aaggcgctgc gccccctgct ccgccgcgcg ctgcccgagc
     3061 gccacggtag gaacgtcgtc gacgacggcg agggtttccg cgtcagcggc tagcgtcgga
     3121 gggcgccctc agtcggcgtg gaagatgacc tcggcgacct tcatctcgcc gtcgacaggg
     3181 cgcgcgacca tcgacatcac cgggatgtcg gacgcgcggt cgaccacgac gcactgatag
     3241 ctgcccgcgc cgagatcctc gttgagcgag cagcgctgga ggcgcgtgct gtcgcggctg
     3301 cggaccggca cgaggaacgc catgtggacg gcgtcctccg tcccgaccgt gagcgcgagc
     3361 ggccgatcgt tcgtgaccca cgccgagaag aacgtcgcgg cgacgactcg cgcctgtcgg
     3421 agcggtgcct ccgacggcga tggggatggc gactgggtcg gtgacggtga gggcgacggt
     3481 gagaccgtcg gcgtctcggg cgacggcgac gggctcggcc cggtcacgcc ggcgggctct
     3541 cgccgcaggt cgaggaccca gagcgtcccc gcgacgatga gcgcggcgac gacgatcccg
     3601 ccggcgatga tccagggccc ccttcgcacg gcgccactgt agccggaggc cgccggctga
     3661 ggcggcggtt cgccctgcta gcatcctcgt gtcatgggtt ccgacgacca ccgcgtccgg
     3721 ttcaggccgc tcgacccgct cgacgtcgat gcgctcctgt cggacgagga gcgcgccgtc
     3781 cgcgatacga cccgcgactg ggtgaagagc cgcgtgctcc ccgagatcgc agagtggttc
     3841 gagcgcggcg agatcccgtc ccgcgagctc gcgaaggagc tcggcgggat ggggctcctc
     3901 gggatgcacc tctccgggta cggaaccgcg ggcgcgagcg cggtgatgta cgggctcgcc
     3961 gtgcacgagc tcgagtacgg cgactccggc gtgcgcagcc tcgtctccgt gcagggctcg
     4021 ctcgcgatgt tcccgatctg gaagttcgga tccgatgagc agaaggatga gtggctgcct
     4081 cggatggcgt cgggcgacgc catcgggtgc ttcgggctca cggagcccga catcggcagc
     4141 gacccggcga gcatgcggac gtcggcgcgc cgcgacgggg acgactgggt gctctccggc
     4201 acgaagatgt ggatcacgaa cggcggcatc gccgacgtcg cggtcgtgtg ggcgcggacc
     4261 gacgacggcg tccgcggctt cgtcgtgccg accgaggcgc cggggttctc gacgaacgag
     4321 atcgagcgga agatctccct gcgcgcgtcg gtcacctctg agctcgtcct cgacgacgtc
     4381 cgtcttcctt cgagcgcgat gcttccaggg gtcaggggga tgcgcggccc gctctcgtgc
     4441 ctcaacgaag cgcggtacgg gatcgtgtgg ggcgcgatgg gtgcggcccg cgcctgctac
     4501 gacgccgcgc tcgagtacgc gacgacgcgc caccagttcg ggaagccgat cggcggcttc
     4561 cagctgaccc agcgcaagct cgtcgacatg gcggtcgaga tcggcaaggg gatgctgctg
     4621 gcactgcacc tcggacgcat gaaggacgag gggcgcgcgg cccccgagca c
//
LOCUS       DASZOB010000277         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_43319, whole genome shotgun sequence.
ACCESSION   DASZOB010000277 DASZOB010000000
VERSION     DASZOB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43319"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VG709_04565"
     CDS             <1..411
                     /locus_tag="VG709_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32382.1"
                     /translation="PLVRYLDDEPSGLAMMLGGLLEANIDAHPERRRLLSPPATVGIV
                     ATDAGVAMTLHVSPAGVTVANGLAGSPAIVVRTDSETLTDLSSVPLRLGLPDATKPAG
                     RAVLGKLRSRSLTVSGMFTHPVLLSRLNRLLSVA"
     gene            573..1961
                     /locus_tag="VG709_04570"
     CDS             573..1961
                     /locus_tag="VG709_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWC32383.1"
                     /translation="MVVAKADGNTVLVGLLEHPATSLDEHLGAGGGRGLERALSLPRG
                     RVVEEVALSGLRGRGGAGFPTGRKWLAVASTGTGERFVVCNAAEGEPAAFKDRYLIRH
                     NPYRLIDGISIAAYAVGANRAYLALKDVFREEAETLAGALQEFQEAGALPVPIELVLG
                     PDHYLLGEETGLLEAIEERAPLPRVARPYMFGLFARPPDENPTLVNNVETLSNVPYIL
                     AEGPDWLRAEGTDASPGTMLFTVAGDVEREGVYERPLGTSLGEVIASAAGGVRGGGSL
                     KVVFPGASNTALVPEQLETPLAFESMAAVGSGLGAGGFAVYDDTTCIVEVALLFCRFL
                     HVESCAQCPPCKLNSGDVVEFLDGLERGRAGGDLDVALARARAATDGQKCGLPTGTSL
                     LMQSLMLLFTDEFRAHRGRACPSPRALVLPKIVDLDEEQGRFRYDERYGAKRPDWTYG
                     STDGDAGSFATS"
     gene            2035..>2700
                     /gene="meaB"
                     /locus_tag="VG709_04575"
     CDS             2035..>2700
                     /gene="meaB"
                     /locus_tag="VG709_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWC32384.1"
                     /translation="MDVGSLVERVLAGDRRAVARAISMVEDGSEGLPELIGGLYPHTG
                     NAHTIGLTGSPGVGKSSLAAELVKAARARDLTVSVLAIDPTSPFTGGALLGDRLRMQT
                     HATDPGVFIRSMATRGHLGGLALAAPEAVRVLDAAGADVILLETVGVGQAEVEVVKQA
                     DTTLVAVAPGWGDAVQASKAGILEIADVFAVNKADREGAGETVRDLETMLRLGPKLEW
                     TPPI"
BASE COUNT          373 a          945 c          986 g          396 t
ORIGIN      
        1 ccgttggtcc gctacctcga cgatgagccg agcgggctcg cgatgatgct cggcgggttg
       61 ctcgaggcga acatcgacgc ccacccggaa cgccggaggt tgctctcgcc gccggcgacc
      121 gtcggcatcg tcgccacgga cgcgggcgtc gcgatgacgt tgcacgtctc gccggccggc
      181 gtcacggtcg cgaacggcct cgcgggctcg ccggcgatcg tcgtccggac cgactcggag
      241 acgctcaccg acctctcgtc cgtacccctg cggctcggac tgcccgacgc gaccaagccg
      301 gcgggccgcg cggtgctcgg gaagctgcgg tctcggtcgc tgacggtgag cggcatgttc
      361 acgcacccag tgctgctgtc gcggctgaac cgcctgctct cggtagccta gagcgcgaac
      421 gagaagggcc ggcccgaggg ccgcccttcg ggatcagagc cgacgagacc gattttgact
      481 attcgaccct gcggttgctc tctgctgcca gagcggcgcg cgaatttcgc ggaacggcag
      541 tggcccgagg gcgtccacgc gccgcgcgtc gcatggttgt cgcgaaggcg gacgggaaca
      601 cagtccttgt gggccttctc gagcatccgg cgacgtcgct ggacgagcat ctcggcgccg
      661 gaggcggccg cgggctcgaa cgcgcgctat cgctgccgcg cgggcgcgtc gtcgaggagg
      721 tggccctgag tgggctccgc ggccgtggcg gagcggggtt ccccacgggg cggaagtggc
      781 tcgcggtcgc gtcgacgggc accggagagc ggttcgtcgt gtgcaacgcc gcagagggcg
      841 agcccgccgc gttcaaagac cggtacctga tccgccacaa cccgtaccgg ctgatcgacg
      901 ggatctcgat cgccgcctac gccgtagggg cgaaccgcgc gtacctcgcg ctcaaggacg
      961 tcttccgcga ggaagcggag acgttggcgg gggcgttgca ggagttccag gaggcgggtg
     1021 ccctccccgt tcccatcgag ctggtgctgg gccccgacca ttatctgctg ggtgaggaga
     1081 cggggctcct cgaggcgatc gaggaacgag ctccgcttcc gcgtgtcgcc cgtccgtaca
     1141 tgttcgggct gttcgcccgc ccgcccgacg agaacccgac gctcgtcaac aacgtcgaga
     1201 cgctgtcgaa cgtgccgtac atcctcgcgg aggggcccga ctggctccgt gcggagggaa
     1261 ccgacgcctc ccccggcacg atgctgttca cggtcgccgg tgacgtcgag cgcgagggcg
     1321 tgtatgagcg accgctcggg acgtcgctcg gcgaggtcat cgcatccgcg gcaggcggcg
     1381 tccgcggagg aggatcgctg aaggtcgtgt tccccggcgc gtcgaacacg gcgctcgtcc
     1441 ccgagcagct cgagacgccc ctcgccttcg agtcgatggc ggcggtgggc tccgggctcg
     1501 gggcgggcgg cttcgccgtg tacgacgaca cgacctgcat cgtcgaggtc gcgctcctct
     1561 tctgtcggtt cctccacgtg gagtcctgcg cccagtgccc accgtgcaag ctgaactcgg
     1621 gagacgtcgt cgagttcctg gatggcctcg agcgcggtcg cgcgggcggc gacctcgacg
     1681 tcgccctcgc acgggcgcgc gcggccacgg acggacagaa gtgcggtctc ccgaccggca
     1741 ccagcctgct gatgcagagc ctgatgctgc tcttcacgga cgagttccga gcccatcgcg
     1801 gacgggcatg cccgtcgccg cgcgcgctgg ttctcccgaa gatcgtcgac ctcgacgagg
     1861 agcaaggccg tttccggtac gacgagcggt acggggcgaa gcgacccgac tggacatacg
     1921 gatcgaccga cggcgacgcc ggatccttcg ctacgtcgta gcgggtcgcc gccgcccgcc
     1981 tccccatacc catctcgccg gttgccgtgc gcgcgcgagg gtacgcttcc ggccgtggac
     2041 gtcgggtcgc tcgtcgaacg agtgctcgcc ggggaccggc gcgcggtcgc gagggccatc
     2101 tcgatggtcg aggacggctc cgaggggctt cccgagctga tcgggggcct ctacccgcac
     2161 accggcaacg cgcacacgat cgggctgacc gggtccccgg gggtcgggaa gtcgagcctc
     2221 gcggcagagc tcgtgaaggc ggcgcgggcc cgcgacctca cggtttccgt gctcgcgata
     2281 gacccgacgt cgccgttcac cggcggggcg ctcctcggcg accgcctccg aatgcagacc
     2341 cacgccaccg acccgggcgt gttcatccgg tcgatggcca cccgcggaca cctgggaggg
     2401 ctcgcgctgg ccgcgcctga ggccgtccgc gtcctcgacg ccgcgggcgc cgacgtgatc
     2461 ctgctcgaga ccgtcggggt cggccaggcc gaggtcgagg tcgtgaagca ggccgacacg
     2521 acgctcgtcg cggtcgcgcc gggatggggc gacgccgtcc aggcgtcgaa ggccgggatc
     2581 ctcgagatcg cggacgtctt cgcggtcaac aaggccgacc gcgagggcgc gggcgagacg
     2641 gtccgcgacc tcgagacgat gctccggctc ggcccgaagc tcgagtggac gccgccgatc
//
LOCUS       DASZOB010000278         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_167199, whole genome shotgun sequence.
ACCESSION   DASZOB010000278 DASZOB010000000
VERSION     DASZOB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167199"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..582
                     /locus_tag="VG709_04580"
     CDS             94..582
                     /locus_tag="VG709_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWC32385.1"
                     /translation="MKVEVVRSPRRRKTITAERRGGRVVVRLPAGMTAAEERTWIDRM
                     VTRIAERERLARLNARRDLERAADRLNRRYFDGRLRWVTIRYVSNHRDRFASCTPDDR
                     TIRISDRIAEMPTWVRDYVVMHELAHLVVPSHSPAFWRLVDRYPLAERARGFLMARGL
                     DE"
     gene            complement(588..1211)
                     /gene="upp"
                     /locus_tag="VG709_04585"
     CDS             complement(588..1211)
                     /gene="upp"
                     /locus_tag="VG709_04585"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWC32386.1"
                     /translation="MPPVVVDHPLVQHLLSSLRDRRTSPPLFRTLTRRLTLALALEAT
                     RDLPAETRQVETPLETTEGRFLGPNLVAIPILRAGLGMLEAVTDAFPDVSVGYIGLER
                     DDDTLQPTSYYEKLPAVSGRHVLLLDPMLATGGSAVRAANSIEEKQPASLRMVCVVAA
                     PEGVRAMEEAHPSVAIVTASLDRQLNSRGYILPGLGDFGDRLYGTEP"
     gene            1316..2386
                     /locus_tag="VG709_04590"
     CDS             1316..2386
                     /locus_tag="VG709_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWC32387.1"
                     /translation="MRSLRALALLGALAIVGAACGGATPEGGGGGGTDDEASDVRVGM
                     ALDVGGLRDKSFNDAAYAGLQRAIEEGLVSEENVELLEANQTGSNRDENITTLAEQGM
                     DIVIGVGFAFSEHVAEIAPEFPDVNFAIIDGFATFVNEDATNIADLTFKEHEGSFLVG
                     AAAGLKTQTQTVGFLGGQQGTGLIERFQAGYEAGVAETCADCEVLVEYIGDSTQAFVN
                     PTQGEALSEKMYDQDADVIYHAAGQSGLGLFKEAVEQGPEALAIGVDSDQYLTASPEE
                     QQHIVTSMLKRVDTAVYEAIKDQVEGTFEGGQQVFGLAEEGVDYADSNPELLPEDIQA
                     QLDEYKQQIIDGEITVPEEPQG"
BASE COUNT          386 a          798 c          905 g          339 t
ORIGIN      
        1 ctcccggaag ggtgcccggc ccctcgcttt cgcgcggcct ctgacctgcc ggtttgtcga
       61 tgtcaccgcc gttcctagaa tccgtgccac gccatgaagg tcgaggtcgt caggagcccg
      121 cgacgcagga agacgatcac ggctgagcgg cgtggtggcc gcgtcgtcgt caggctcccg
      181 gcggggatga ccgccgccga ggagcggacc tggatcgacc gcatggtcac tcggatcgcc
      241 gagcgggagc ggctcgcccg gctcaacgcc cgccgggacc tcgagcgcgc ggcggatcgg
      301 ctgaaccggc gctacttcga cgggcgcctc cggtgggtga cgatccgcta cgtctccaac
      361 caccgcgacc gcttcgcctc atgcacgccg gacgaccgga cgatccgcat ctccgatcgc
      421 atcgcggaga tgccgacgtg ggtccgggac tacgtcgtga tgcacgagct cgcgcacctc
      481 gtcgtgccga gccattcgcc ggcgttctgg cggctcgtcg accgctaccc gctcgccgaa
      541 cgcgcccgtg ggttcctcat ggcgcggggc ctcgacgagt aggcgcccta cggttcggtg
      601 ccgtagagcc gatcgccgaa gtctccgagc cccggaagga tgtacccgcg gctgttcagc
      661 tggcgatcga gcgacgcggt cacgatggcg accgaggggt gtgcctcctc catcgcgcgg
      721 acgccttcgg gggcggccac gacgcagacc atccggagcg aggccggctg cttctcctcg
      781 atgctgttgg ccgcgcgtac cgcggagcct ccggtcgcga gcatcgggtc gagcaagagg
      841 acgtggcgcc cggagacggc cgggagcttc tcgtagtagg aggtcggttg gagcgtgtca
      901 tcgtcgcgct cgagcccgat gtagccgacc gagacgtctg ggaaggcgtc ggtgaccgcc
      961 tccagcatgc cgaggcccgc ccgcaggatc gggatcgcga cgaggttcgg tccgaggaat
     1021 cgtccctcgg tcgtctcgag cggggtctcg acttgtctcg tctcggcggg caggtcgcgc
     1081 gtcgcctcga gagccagcgc gagggtgagc cgccgcgtca gcgtccggaa gagcggcggc
     1141 gacgtccgcc ggtcgcggag cgaggacagc aggtgctgga ccagcgggtg gtcgacgacc
     1201 acaggcggca tcgcggcgca tcctaggccg ttttgacgag ggcttcgcgg tggacggaga
     1261 atcgcgggcg gagccggcgt ggggagtcgc cggccggagg agagagggga gacgtatgcg
     1321 atcactgcgt gcgttggcgc tgctcggcgc cctcgcgatc gtcggtgcgg cgtgcggcgg
     1381 cgcgacgccc gagggcggcg gcggaggcgg aaccgacgac gaggcgtcgg acgtccgcgt
     1441 cggcatggcg ctcgacgtcg gtgggctccg cgacaaatcg ttcaacgacg ccgcgtacgc
     1501 cgggctccag cgcgccatcg aggaggggct cgtcagcgag gagaacgtcg agctgctcga
     1561 ggccaaccag accgggtcga accgcgatga gaacatcacg acgctcgcgg agcagggcat
     1621 ggacatcgtg atcggggtcg ggttcgcgtt ctcggagcac gtcgctgaga tcgcgccgga
     1681 gttccccgac gtcaacttcg cgatcatcga cggcttcgcc acgttcgtga acgaggacgc
     1741 cacgaacatc gcggacctca cgttcaagga gcacgagggc tcgttcctcg tcggcgccgc
     1801 ggccggtctc aagacccaga cgcagaccgt ggggttcctc ggcgggcagc aggggacggg
     1861 gctgatcgag cggttccaag ccgggtacga ggccggcgtc gccgagacgt gcgccgactg
     1921 cgaggtcctc gtcgagtaca tcggcgactc gacccaggcg ttcgtcaacc cgacccaagg
     1981 cgaagccctg tccgagaaga tgtacgacca ggacgcggac gtgatctacc acgcggcggg
     2041 ccagtccggg ctcggcctct tcaaggaggc cgtcgagcag gggcctgagg cgctggcgat
     2101 cggggtcgac tccgaccagt acctcacggc cagccccgag gagcagcagc acatcgtcac
     2161 ctcgatgctc aagcgcgtcg acaccgcggt gtacgaggcg atcaaggacc aggtcgaggg
     2221 gacgttcgag ggcggccagc aggtgttcgg gctcgccgag gagggcgtcg actacgccga
     2281 cagcaacccc gagctgctgc ccgaggacat ccaggcccag ctcgacgagt acaagcagca
     2341 gatcatcgac ggcgagatca cggtccccga ggagcctcag ggctaacccg cgtgaggcgg
     2401 gacggcgaga ccggggcggg gggggggg
//
LOCUS       DASZOB010000279         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_126740, whole genome shotgun sequence.
ACCESSION   DASZOB010000279 DASZOB010000000
VERSION     DASZOB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126740"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VG709_04595"
     CDS             complement(<1..524)
                     /locus_tag="VG709_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWC32388.1"
                     /translation="MARTSFKAMSCSVARTLDVVGERWTPLIVRDILIGVTRFDAIQR
                     DLGISRKVLAERLSELTERGVLERVRYQDNPPRHDYVLTRKGVDLAYVLIAMKTWGDR
                     WASEDTGPPMLLRHERCGAIADIVPACSGCGEAIAPGEVTPLPGPGAEPGPGTTEIFA
                     ALTRLGEARDVPGA"
     gene            586..>2216
                     /locus_tag="VG709_04600"
     CDS             586..>2216
                     /locus_tag="VG709_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC32389.1"
                     /translation="MLERIADLTWRRPKLVLGLVALFMVVAVAAGRDVEQHLKAAGFA
                     DSESESERATALLRDALGYDANPGIVVLVRAPDGGRLDVRDPGVRREVGRISSQLAKA
                     EHVGRVVDPVRSGRRGRALIARDGHSLVITGHLSTQDVEEDGGQAAEDAQERIASDTL
                     DVSMGGFAPSFNEVNDQTRSDLTKAELIAFPVLAVLLLLVFRGVVAAGIPLLIGVVSI
                     LGTFLVLRVMATFVDTSLFALNIATGLSLGLAVDYALLLVSRYREELEKDGATRDAHR
                     NTVLTAGRTTVFSGFTVAVAMAALGILPQRFLYSMAVAGAAVALLSALIAILVVPSLL
                     SLLGDRVNALAVRRGPAVSDESTGWYRLARGVMRRPLPVALATFAVLIAAAVPLLSTV
                     LTGPSAEAVPPTQPSYDTNEYVEAHYARGVVEAMTITVDGRASDGDLKRLSAEISAVD
                     GAGRAPPFTRASEDVAFVNVAPEQPALSRRSQDAVDRIRELDPPRASSIVVSGNTARF
                     MDQKQSLADHLPLVVAIVAAATFVLLFLLTGSIILP"
BASE COUNT          298 a          796 c          773 g          349 t
ORIGIN      
        1 tccgcgcccg ggacgtcccg agcctcgccc agtcgcgtca aggccgcgaa gatctcggtg
       61 gtacccggtc cgggctccgc gccgggcccg ggcagtgggg tgacctcgcc cggcgcgatg
      121 gcctcgccgc agccagagca cgccggcacg atgtcggcga tcgcgccaca gcgctcgtgg
      181 cggagcagca tgggcgggcc ggtgtcctca gaggcccagc ggtcgcccca ggtcttcatg
      241 gcgatcagca cgtaggcgag gtcgacgccc ttcctcgtga gcacgtagtc gtggcgcggc
      301 gggttgtcct ggtagcggac gcgctcgagc acgccgcgct cggtcagctc ggagaggcgc
      361 tcggccagga ccttgcgcga gatcccgagg tcccgctgaa tggcgtcgaa gcgtgtgacg
      421 ccgatcagga tgtcgcgcac gatgagcggc gtccaccgct cgcccacgac gtcgagcgtg
      481 cgcgccaccg agcagctcat tgccttgaag gacgttcgcg ccatcgtccg atcgtagcga
      541 aagtgggttc ctttaaggaa ctcactttgg taggctcgcg cgcccgtgct cgagcgcatc
      601 gccgatctca cctggcgccg ccccaagctg gtactcgggc tcgtcgccct cttcatggtc
      661 gtcgccgtcg ccgccggacg cgacgtcgag cagcacctca aggccgcggg gttcgcggac
      721 agcgagtccg agagtgagag ggctacggcg ctcctgcgcg acgcgctcgg ctacgacgcc
      781 aacccgggca tcgtcgtcct cgtccgcgcg cccgacggcg gccgcctcga cgtgcgcgat
      841 ccgggcgtcc gtcgcgaggt cggccgcatc agcagccagc tcgccaaagc cgagcacgtc
      901 ggccgcgtcg tcgatccggt ccgcagcggg cgcaggggcc gcgcgctcat cgcccgggat
      961 gggcactcgc tcgtgatcac gggccacctc tcgacgcagg acgtcgagga ggacggcggc
     1021 caggccgccg aggacgcgca ggagcggatc gcctcggaca cgctcgacgt gtcgatgggc
     1081 ggcttcgcac cgagcttcaa cgaggtcaac gaccagaccc gcagcgacct aacgaaggcc
     1141 gagctgatcg ccttccccgt gctcgccgtg ctgctgctgc tcgtgttccg aggcgtcgtg
     1201 gccgccggga tcccgctgct gatcggcgtc gtctcgatcc tcggcacgtt cctcgtgctg
     1261 cgggtcatgg cgaccttcgt cgacacctcg ctgttcgcgc tgaacatcgc cacggggctc
     1321 agcctgggcc tcgccgtcga ctacgcgctg ctgctggtgt cccgctaccg ggaggagctg
     1381 gagaaagatg gcgccacccg cgacgcgcac cggaatacgg tgctcaccgc cggacggacc
     1441 acggtgttct ccggattcac cgtcgcggtc gcgatggccg cgctcggcat cctgccccag
     1501 cggttcctgt actcgatggc cgtcgcaggc gccgcggtgg cgctgctctc cgcgctcatc
     1561 gcgatcctgg tcgtgccgtc gctcctgtcg ctcctcggtg accgggtgaa cgcgctcgcg
     1621 gtgcggcgcg ggcccgccgt ctccgacgag tcgaccggct ggtaccggct cgcgcgcggc
     1681 gtcatgcggc gaccgctccc ggtggctctc gcgacgttcg ccgtgttgat cgcggcggcc
     1741 gtgcccttgc tgtcaacggt gctcaccggt cccagcgcgg aggcggtgcc gccgacgcaa
     1801 ccgtcatatg acaccaacga gtacgtcgag gcccattacg cgcggggtgt ggtcgaggcg
     1861 atgacgatca cggtggacgg cagggcaagc gacggcgacc tgaagcggct gagcgccgag
     1921 atctccgcgg tggacggggc gggtcgggcg ccgcctttca cgcgcgcctc ggaggacgtc
     1981 gccttcgtga acgtggctcc ggagcaaccc gcgctttcgc ggcgctcgca ggacgccgtc
     2041 gacaggatcc gcgaactcga tccgccgcgc gcgtcatcga tcgtcgtctc gggcaacacg
     2101 gcacggttca tggaccagaa acagagcctc gcggaccatc tgccgctcgt cgtggcgatc
     2161 gtggcggcgg cgaccttcgt cctgctcttc ctcctgacag gctcgatcat cctgcc
//
LOCUS       DASZOB010000280         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_96792, whole genome shotgun sequence.
ACCESSION   DASZOB010000280 DASZOB010000000
VERSION     DASZOB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96792"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VG709_04605"
     CDS             complement(<1..475)
                     /locus_tag="VG709_04605"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWC32390.1"
                     /translation="MSAAEPGTVLVTGGAGFVGSHVCDRLLAEGHRVVAADDLTTGRI
                     ANLAEARSYGKAFSFENVDIRADVLDALFRRYRPEVVMHLAAQPSVAVSTRDPLYDAD
                     VNILGTINLLACAARAGTRKLVFATSGGTIYGEPRRLPVKETALAGSHPRSPYGAS"
     gene            complement(472..1476)
                     /gene="tsaD"
                     /locus_tag="VG709_04610"
     CDS             complement(472..1476)
                     /gene="tsaD"
                     /locus_tag="VG709_04610"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWC32391.1"
                     /translation="MRVLGIETSCDETAVAVVEDGLTLRSNVLSSQHEIHDRFGGVVP
                     ELASRAHVERLTPLIDVALSEAGTTWRDVDAVAVTRGPGLSGALLVGLATAKAVALAL
                     DVPLVAVNHLEGHIYANFLEHGRVEPPYVVLLVSGGHTMLVHVPEEHRYEVLGQTLDD
                     AAGEAFDKVARFLGLGFPGGPALDRLARKGNPEAVAFPRAMATSGDFDFSLSGLKTAV
                     IRHVRAEDAGGRETSLEDLAASFQEAVVDVQVQKTLEAAEVTGVRTVLLGGGVVANSR
                     LRERISAEAEGRGLRVLYPSPVLCTDNGAMIASAGYFRLARGERSDLETTVEPGLPLA
                     "
     gene            complement(1473..1967)
                     /gene="rimI"
                     /locus_tag="VG709_04615"
     CDS             complement(1473..1967)
                     /gene="rimI"
                     /locus_tag="VG709_04615"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWC32392.1"
                     /translation="MRRRHLRGIMAIERQVYPRPWSPTLFLSEMNAGGARAYIVAILD
                     RQVVGYGGLVGYGDEAHITTIAVDPEHHRKKIGTRLMYELVARARELGARAVSLEVRV
                     TNWGAQRLYARFGFRPVGVRKNYYQETGEDALVMWTDDIRTSDYARVLEHVVSEVPEG
                     LRPT"
     gene            complement(1984..>2309)
                     /locus_tag="VG709_04620"
     CDS             complement(1984..>2309)
                     /locus_tag="VG709_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWC32393.1"
                     /translation="PVPGGVARETEYAVGPPERLAADLEASGEETLLVGGGALLYRRA
                     LEDAGTGLEIGSVAHAFPSAESLLELAIPRFEREEAVRPDQVVPLYLRKSDAEIGWAK
                     RTRSA"
BASE COUNT          327 a          851 c          821 g          310 t
ORIGIN      
        1 tcgaggcgcc gtacggcgac cgggggtggc tcccggcgag cgccgtctcc ttcaccggaa
       61 gccgccgcgg ctcgccgtag atcgtgccgc cggacgtcgc gaacacgagc ttccgcgttc
      121 cggcgcgcgc cgcgcacgcg aggaggttga tcgtcccgag gatgttgacg tcggcgtcgt
      181 acagcgggtc ccgcgtcgag accgcgacgc tcggctgcgc ggcgaggtgc atcacgacct
      241 cgggacggta gcgccggaac agcgcgtcga ggacgtcggc gcggatgtcg acgttctcga
      301 agctgaacgc cttgccgtac gaccgggcct cggcgaggtt cgcgatccgc ccggtcgtga
      361 ggtcgtcggc cgcgacgacg cggtggccct cggcgaggag ccggtcgcag acgtgcgagc
      421 cgacgaaccc ggccccgccg gtgacgagga cggtacccgg ctcggcggcg ctcatgcgag
      481 cgggagcccg ggctcgaccg tcgtctcgag gtcggagcgc tcgcctcgcg cgaggcggaa
      541 gtacccggcg gacgcgatca tcgcgccatt gtcggtacag agcaccggcg agggatagag
      601 caccctgagg ccgcggccct ccgcctcggc gctgatccgc tcgcggagcc gcgagttcgc
      661 cacgacgccg ccgccgagca gcacggtccg cacgccggtg acctcggcgg cctcgagcgt
      721 cttctggacc tgcacgtcga ccacggcctc ctggaacgag gccgcgaggt cctccagcga
      781 agtctcgcgt ccgccggcgt cctcggcgcg gacgtgccgg atcaccgccg tcttcaggcc
      841 cgacagcgag aagtcgaagt cgcccgacgt cgccatcgcg cgcgggaacg cgactgcctc
      901 cgggttgccc ttccgcgcca ggcggtcgag ggccggtccc cccgggaacc cgagcccgag
      961 gaaccgggcg accttgtcga acgcctcgcc ggcggcgtcg tcgagcgtct ggccaaggac
     1021 ctcgtaccgg tgctcctcgg gcacgtggac gagcatcgtg tggccgccgg agacgagcag
     1081 caccacgtac ggcggctcga cgcggccgtg ctcgaggaag ttcgcgtaga tgtgaccctc
     1141 gaggtggttg accgcgacga gcggcacgtc gagcgcgagc gcgacggcct tggcggtcgc
     1201 gaggccgacg agcagcgcgc ccgacagccc gggcccgcgc gtcacggcga ccgcgtcgac
     1261 gtcgcgccac gtcgtcccgg cttccgagag cgcaacgtcg atcagcggcg tcagccgctc
     1321 gacgtgcgcg cgcgacgcga gctcgggcac caccccgccg aagcggtcgt ggatctcgtg
     1381 ctggctcgag agcacgttcg agcgaagcgt cagaccgtcc tcgaccaccg cgacggccgt
     1441 ctcgtcgcag gacgtctcga tgccgaggac cctcacgtcg ggcggagccc ctccggcacc
     1501 tctgagacga cgtgctcgag cacccgcgcg tagtccgacg tccggatgtc gtcggtccac
     1561 atcacgaggg cgtcctcgcc ggtctcctgg tagtagttct tccggacgcc gaccgggcgg
     1621 aacccgaacc gcgcgtagag gcgctgcgct ccccagttgg tgacgcgcac ctcgagcgat
     1681 acggcgcgcg ccccgagctc tcgagccctc gcgacgagct cgtacatgag gcgggtcccg
     1741 atcttcttcc ggtgatgctc gggatcgacg gcgatcgtcg tgatgtgcgc ctcgtcgccg
     1801 tatccgacca gcccgccgta cccgacgacc tgccggtcga ggatcgcgac gatgtacgcg
     1861 cgggcgccac cggcgttcat ctcggagagg aacagcgtcg ggctccacgg ccgcgggtac
     1921 acctgacgct cgatcgccat gatcccgcgg aggtggcgcc gccgcatccg cgcgatctcg
     1981 agctcaggcg ctccgcgttc gcttagccca accgatctcc gcatcggact tcctcaggta
     2041 gagcggcacg acctggtcgg gccgcacggc ctcctcgcgc tcgaaccgcg ggatcgcgag
     2101 ctcgagcagg gactcggccg acgggaaggc gtgtgcgacc gaaccgatct cgaggcccgt
     2161 acccgcatcc tccagcgctc gacggtacag cagcgcgccg ccgccgacga ggagcgtctc
     2221 ctcgccgctc gcctcgaggt cggccgcgag ccgctcggga ggaccgaccg cgtactcggt
     2281 ctcgcgcgcg acgccaccgg gtaccgggc
//
LOCUS       DASZOB010000281         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_12841, whole genome shotgun sequence.
ACCESSION   DASZOB010000281 DASZOB010000000
VERSION     DASZOB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12841"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1483)
                     /locus_tag="VG709_04625"
     CDS             complement(179..1483)
                     /locus_tag="VG709_04625"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32394.1"
                     /translation="MPRQKSTHVDDPRAVGERLKAARESAGLSQRQLAFPGCSPAYIS
                     RIEAGDRIPSLQLLRELGRRLGVTEDYLATGNAARSEQFSLVEAEIALRLDDLDRARE
                     LYSAALERAATPHNRSLAEEGFGHLAFRSGDLREAIRLFEQALVTSGDEDWERTSLAE
                     SLGRAYALVGELEPCIAIFERCLAAFERTDDHVETIRFSCLLGYALSDSGDFDRAEAV
                     VTQALAAGRDSYDPYMRARVYWSKAKLRIDQGDADAASRYAYQALAALELTEDLHYTG
                     LAHQLIAHIELERDRPDEALEHLEEGWRLVERTGTPIERAHFLVEEARALARVGDRER
                     AALLAAEIIGLLDDAEPGEVGRVYAVLADLALGLDDRERAGELYKTAVEFLERNTPNR
                     ELVEVYAKLANLLEEDGRADEAYAYMKRAVGMKQAVTPRRTV"
BASE COUNT          227 a          563 c          581 g          268 t
ORIGIN      
        1 gcccggaggg cggcggcggc gaggccgtcc gtagcctccg acgtgggagt cggatcgcgg
       61 atcgcttctt gcatgcgtgc agcgcgagct gcgctgcgct cagatgagga gagcctgcgc
      121 tacccgttcg cgcggctacg cgaactatac aacaagcgga gcgcgatccg agggcgctct
      181 acaccgtgcg tctcggcgtc acggcctgct tcatgccgac ggcccgcttc atgtaggcgt
      241 acgcctcgtc ggcgcgaccg tcctcctcga gcaggttggc gagcttcgcg tacacctcga
      301 cgagctcgcg gttcggcgtg ttcctctcga ggaactcgac cgcggtcttg tacagctcgc
      361 cggcgcgctc gcgatcgtcg agcccgagcg ccaggtccgc gaggaccgcg tacacgcggc
      421 cgacctcgcc gggctcggcg tcgtcgagga ggccgatgat ctcggctgcg agcagggcgg
      481 ccctctccct gtcgccgacg cgggcgagtg cgcgtgcttc ctctacgagg aagtgagcgc
      541 gctcgatcgg cgttccggtg cgctccacca ggcgccatcc ctcctcgagg tgctcgagcg
      601 cctcgtcggg gcggtcgcgc tcgagctcga tgtgcgcgat cagctggtgc gcgaggcccg
      661 tgtaatggag atcctccgtg agctcgagtg ccgcgagcgc ctggtaggcg taccgcgacg
      721 cggcgtcggc gtccccctgg tcgatgcgaa gcttggcctt cgaccagtac acgcgggcgc
      781 gcatgtacgg gtcgtagctg tcgcgtcccg cggcgagcgc ctgcgtcacg accgcctcgg
      841 cgcggtcgaa gtcgccgctg tccgagaggg cgtatccgag caggcaggag aagcggatcg
      901 tctcgacgtg gtcgtccgtc cgctcgaacg cggcgagaca ccgttcgaag atggcgatgc
      961 agggctcgag ctcgcccacg agcgcgtagg cgcgtccgag gctctcggcg agcgaggtgc
     1021 gctcccagtc ctcgtcgccg gacgtcacga gcgcctgctc gaagagccgg atcgcctcgc
     1081 gcaggtctcc gcttcggaac gcgaggtggc cgaagccctc ctcggccagg gagcggttgt
     1141 gcggcgtcgc cgcacgttcc aacgcggccg agtacagctc gcgggcgcga tcgaggtcgt
     1201 ccaggcgcag cgcgatctcc gcctcgacga gcgagaactg ctccgagcga gcggcattgc
     1261 ccgtcgcgag gtagtcctcg gtcacgccga gccggcggcc gagctcgcgg aggagctgca
     1321 gggacgggat gcggtccccc gcctcgatcc gcgagatgta cgccggcgag cagccgggga
     1381 aggcgagctg ccgctgactg agaccggcgc tctctcgcgc ggccttcagg cgctcaccga
     1441 cggcccgcgg gtcgtcgacg tgggttgact tctgtctcgg caaattgatc gccccttccg
     1501 tcgttaagcc ttgtatactc ccgcaccagt tccgggcccg gggctgtcca gtgacattcg
     1561 agaaaaggac ggtcccaaga tgaaggtacg cctcctcatg ctggccacgt cgtcggccct
     1621 gtacgcctcg tttctcgga
//
LOCUS       DASZOB010000282         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_76365, whole genome shotgun sequence.
ACCESSION   DASZOB010000282 DASZOB010000000
VERSION     DASZOB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76365"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VG709_04630"
     CDS             <1..1239
                     /locus_tag="VG709_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWC32395.1"
                     /translation="VGDDETGLEGLELEHDDLLAGRDGSVLLEQDPSGNAIPQGMRRT
                     LDPVAGDDLVLSIDGDLQFQAQRALAAAVRSNDARGGMAIVMDPRTGEILAMATDPGF
                     DANRFAETSLERRRNRAVTDVYEPGSVNKVITASAAIQEHAVGLRQTFTVPDRYRVSD
                     EVFNDAHRHPPMTMTLTDIIADSSNVGTIMTAQRVGKRTLDRYLRRFGFGEPTGVGFP
                     GEAAGILPEAEAWWGSSMGTIPIGQGVAVTPLQMTAVYATIANRGVWVRPTLVRGTLG
                     PDGDVIPFGERERQRIVSRRTARAVTGMLARAVESGTGEAAQIGGYWVAGKTGTARKP
                     LEGGLGYSDRYVASFMGFLPASRPQLVVAAILDEPITVYGGVASAPLFREIARFAIAH
                     LRIPPGERPWIPPVAGERGR"
     gene            1295..>1789
                     /locus_tag="VG709_04635"
     CDS             1295..>1789
                     /locus_tag="VG709_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWC32396.1"
                     /translation="MPHERPLEDLLAALQRPEVRGDTRVHVAGVAYRSADVGPGWLFF
                     AVPGRTADGHSFAAEAARRGAAVIVVERWLDDPPDAVTQVRVVSVREAMGPVAAAFYG
                     RPADRLAMVGVTGTNGKTTTTYLLERIFEAAGMRAGVIGTTGVRIDRRAVPHDRTTPE
                     APDLH"
BASE COUNT          264 a          623 c          667 g          235 t
ORIGIN      
        1 gtcggggacg acgagaccgg actcgagggg ctcgagctcg agcacgacga cctcctcgct
       61 ggccgcgacg gctcggtgct gctggagcag gatccgtcgg ggaacgcgat ccctcaaggg
      121 atgcgccgga ccctcgaccc cgtggccggc gacgacctcg tgctctcgat cgacggagac
      181 ctccagttcc aagcgcaacg cgcgctcgcc gcggcggtcc gaagcaacga cgcgcgaggc
      241 gggatggcga tcgtcatgga tccgcgcacc ggcgagatcc tcgcgatggc gacggacccg
      301 gggttcgacg cgaatcgctt cgcggagacg tcgctcgagc gaaggcgcaa ccgcgccgtg
      361 accgacgtct acgagccggg ttccgtcaac aaggtgatca ccgccagcgc cgcgatccag
      421 gagcacgccg tcggtctacg ccagacgttc acggtgccgg accggtaccg cgtgtccgac
      481 gaggtcttca acgacgcgca caggcacccg ccgatgacga tgacgctcac cgacatcatc
      541 gcggactcga gcaacgtcgg gacgatcatg acggcgcaac gcgtcgggaa gcggacgctc
      601 gaccggtatc tgcgccgctt cggcttcgga gagccgaccg gcgtcggctt ccccggtgaa
      661 gcggcgggga tcctccccga ggccgaggcg tggtggggga gcagcatggg gacgatcccg
      721 atcgggcagg gcgtcgctgt gacgcctctc cagatgaccg cggtctacgc gacgatcgcg
      781 aaccgcggcg tgtgggtgcg cccgacgctc gtccgcggca cgctcgggcc ggacggcgac
      841 gtgatcccat tcggcgaacg ggaacggcaa cggatcgtct cgcgccggac ggcgcgggcc
      901 gtcacgggca tgctcgcgcg cgccgtcgag tcggggaccg gcgaggccgc gcagatcggc
      961 gggtactggg tcgccggcaa gaccgggacg gcacggaagc cgctggaggg cggcctcggg
     1021 tacagcgacc gctacgtcgc gtcgttcatg gggttcctgc cggcgagccg gcctcagctc
     1081 gtcgtcgccg cgatcctcga cgagccgatc acggtctacg ggggcgtcgc gtcggccccg
     1141 ttgttccggg agatcgcgcg gttcgccatc gcgcatctgc gcatcccgcc tggcgagcgt
     1201 ccgtggatcc cgcccgtggc gggagaacgg gggcggtaga atgccgccgc ccgggaccct
     1261 cagcgtacgg gcatcctcca cgaacgagca cgtcgtgccg cacgagcgcc cgctcgagga
     1321 tcttctcgcc gccctccaga ggccggaggt gcgcggcgat acgcgcgtgc acgtcgccgg
     1381 ggtcgcgtac cgttcggccg acgtgggccc gggatggctg ttcttcgcgg tccccggccg
     1441 tacggcagac gggcattcct tcgcggccga ggcggcgcgc cgcggcgccg ccgtgatcgt
     1501 ggtcgaacga tggctcgacg acccgccgga cgcggtcaca caggtccggg tcgtctccgt
     1561 gcgcgaggcg atggggcccg tcgccgcggc gttctacggg cggccggccg accgcctcgc
     1621 gatggtgggc gtcacgggaa ccaatgggaa gaccacgacg acgtacctcc tggagcggat
     1681 cttcgaggcg gcaggtatgc gcgcgggcgt catagggacg acgggggtcc ggatcgaccg
     1741 ccgcgcggtc ccgcacgacc gcacgacgcc ggaggccccc gacctgcat
//
LOCUS       DASZOB010000283         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_167294, whole genome shotgun sequence.
ACCESSION   DASZOB010000283 DASZOB010000000
VERSION     DASZOB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167294"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VG709_04640"
     CDS             <1..282
                     /locus_tag="VG709_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32397.1"
                     /translation="LVLLLAAMSGVFWTVLKVTIVAVLTTILTLVLLAWIGVWYVKRR
                     FRSFVRDYDREIERRRGGRRDGLDAPGWKHPDEPLPRGEAGPADRQPPA"
     gene            complement(267..617)
                     /locus_tag="VG709_04645"
     CDS             complement(267..617)
                     /locus_tag="VG709_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32398.1"
                     /translation="MDEHGFLHLVDRTKDLVKSGGEWISSLDLETALMAHPDVVEAAV
                     IGVPDEHWSERPLACVVVRPGAEVTADELRAFIAPRFPKWWIPDRVEVVDAIPKTSVG
                     KFDKRALRARYAGG"
     gene            complement(696..1856)
                     /locus_tag="VG709_04650"
                     /pseudo
     CDS             complement(696..1856)
                     /locus_tag="VG709_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            1962..2309
                     /locus_tag="VG709_04655"
     CDS             1962..2309
                     /locus_tag="VG709_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32399.1"
                     /translation="MANGAIEEQGELVWEGALGQTRLRIDILPSGRLTARWRSHGNER
                     RVVVDTEQELERRVLFGLMVGGGGDEQGVAREIMGKVVEAKSSRPDPTQAVRRKKKRT
                     RPSRRGIPRPRRR"
BASE COUNT          327 a          875 c          867 g          328 t
ORIGIN      
        1 ctcgtgttgc tgctggcggc gatgtccggg gtgttctgga cagtgctcaa ggtcaccatc
       61 gtcgccgtcc tgaccacgat cctcacgctc gtcctgctcg cctggatcgg cgtctggtac
      121 gtgaagcgac ggttccgctc cttcgtccgc gattacgacc gagagatcga gcgccgacgg
      181 ggcgggcgac gggacggcct cgacgcgccc ggctggaagc acccggacga gccgttgcct
      241 cgcggcgagg ccggtccggc ggaccgtcag ccgcccgcgt agcgggcccg aagcgcccgc
      301 ttgtcgaact tcccgacgct cgtcttcggg atcgcgtcca cgacctcgac gcgatcgggg
      361 atccaccact tcgggaaccg cggcgcgatg aaggcgcgga gctcgtcggc cgtcacctcc
      421 gcgccgggcc ggacgacgac gcaggcgagc ggtcgctcgc tccaatgctc gtcggggacg
      481 ccgatcacgg ccgcctcgac gacgtccggg tgcgccatga gggcggtctc gaggtcgagc
      541 gaggagatcc actctccgcc cgacttcacc aggtctttcg ttcggtcgac caggtgcagg
      601 aagccgtgct cgtccacgac gccgacgtcg ccggtccgca gccagccgtc gacgagcgcc
      661 tgcctggtgc gttcctcgtc ctcgtagtag ccggccgcga cccacgggcc acggacgcgg
      721 acctcgccgt gcgagacgcc gtcgtgcggc acgtcctgtc cggtgtcctc gtcggcgacg
      781 cggatgcgga tgccggggac gggccggccg accctcgcgc ggagccgcag ctgctcgtcg
      841 agcgggaggg catcatgctc gggcgagagt ctcgacagcg cggcgagcgg ccccgtttcg
      901 gtcatgcccc agccctggcg gatctcgacc ccgagctcgc gttcgtagcg ctcgagcagc
      961 gagaccggca ccggtgcgcc tcccgccttg atcagccgga gggacgagag gtcggcgggg
     1021 tgctcccgga ggtgctggag gagcgcgatc cacaccgtcg ggacgcccgc cgccacggtg
     1081 acgcgctccg ccgcgacgag gtccgccagc gtcgccggat cggccgagcg cggcccggtg
     1141 aacacctgct tggccccgac catcgccgcc gcgtacggga acccccaggc gcccgcgtgg
     1201 aacatcggga ccaccggcat cacgacgtcg cggtcggtca gcccgacggt ggccgcgagg
     1261 ttcgtcatcg tcgcgtgcag gaccacggcg cggtgggagt acacgacgcc cttcggcatc
     1321 ccggtcgtcc cggacgtgta gcccatctac gccgcgtcgc cctcgtcgag ctcgggccac
     1381 tccgcgtccg gatccgcgtc cgtgaggagc tcctcgtagt cgagcgcatc gccgggcagg
     1441 tcgccgccca tcgcgacggt gcgttcgacc gtcttcaggc tcgcggtgac ggcgtcgaag
     1501 cgctcggcga ggtcggcgtc gacgaacacg acgcgatcct cggcgtggtt cgcgacccac
     1561 gcggcctcgt ccgggtgcag gcggatgttg agcgggtgca gcaccgcgcc catcgagggc
     1621 acggcgaggt agagctcgag gtgacgccac gtgttccagc cgtaggtcgc gacgcgggcg
     1681 cccctgcgaa cgccgagcgc tcgcagcgcg ttcgccagtc gggccgcgcg ctcagcgatc
     1741 gccgcatacg aagtccggtg gacgtcgtcc tcgagtctcg tgacgacctc cacgcggggg
     1801 tggtagcggg ccgcgcgctc gaggacgcgc gtgaccagca gctggtcgtc gagcatcagg
     1861 ccccgcacgg gctcgatggt aggacgcccg tggtagtctc ggccgcgacc cccgcgtcct
     1921 gcgcgcgtcc gcgcatgggc gctccgaacg aggagcaacg gttggcgaac ggcgcgatcg
     1981 aggaacaggg tgaactcgtc tgggagggag ccctgggcca gacgcgcctc cggatcgaca
     2041 tcctcccgtc cggccgcctg acggcgcgct ggcgcagtca cggcaacgag cggcgggtcg
     2101 tcgtcgacac cgagcaggag ctcgagcgcc gcgtgctgtt cggcctcatg gtcggcggcg
     2161 gcggcgacga gcagggcgtc gcgcgagaga tcatgggcaa ggtcgtcgag gcgaagtcct
     2221 cgcgccccga tccgacccag gccgtccgcc gcaagaagaa gcgcacgcgc ccgtcgcgcc
     2281 gcggcatccc gcgcccccgc cgccggtagc cgagcgctac ggcaggtcga gcatcccgtc
     2341 cggcgcgatc gcgaacccgg gcacccacgc gacctcccac gcgtggccat ccgggtc
//
LOCUS       DASZOB010000284         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_12861, whole genome shotgun sequence.
ACCESSION   DASZOB010000284 DASZOB010000000
VERSION     DASZOB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12861"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VG709_04660"
     CDS             <1..1388
                     /locus_tag="VG709_04660"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWC32400.1"
                     /translation="PGRRQKIQEIRRRINEQGIKYIFFQQISVSGHVNGKGVSATMWE
                     KVAEDGYQLVYGATADLFVDRQDRYIGFGPEESELAAIADLDTFAQLPWDSRVARVFC
                     DCYDTETGELLEADPRQNLKRTVNEVAQELGYTFLCGIEPEMMWMKRPKGSTDPGAAE
                     GITKPWCYHINQFEELRPIILDVIDYGERLGINPSYGDHEDSPGQLELNFLFDRAVRT
                     ADNLSTYRQLCQAAARKHTREGTYGDDLEVLATFMPKPFTGVSANGAHHHFTLVDEQG
                     QNVFYDADGPAKLSELGRQFMGGILEHARALCGITAPTVNSYKRFWDFGFWAPIYKDY
                     GWQNRTTLVRVASGGRFEFRAVDSSCNPDLTQAALLRAGLDGVKRRLDPGPPQQRNIY
                     DVLAEGQEIERVPEHLGEALDALERDDVGRTALPGRLYDVYMHYTRDEWERFLSAVTD
                     WERERYLDVLP"
     gene            1411..>1637
                     /locus_tag="VG709_04665"
     CDS             1411..>1637
                     /locus_tag="VG709_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWC32401.1"
                     /translation="MCGIAGIIYRDGTRPIGRDMTRMLQSMKHRGPDSTGYALYGAEH
                     DGRVVMRYTLADANTARDFDFHERLERHQRQV"
BASE COUNT          285 a          580 c          542 g          230 t
ORIGIN      
        1 atcccggccg ccgccagaag atccaggaga tccggcgccg gatcaacgag caggggatca
       61 agtacatctt cttccagcag atctccgttt ccgggcacgt caacgggaag ggcgtctcgg
      121 cgacgatgtg ggagaaggtc gccgaggacg gctaccagct cgtctacggg gcgacggccg
      181 acctcttcgt cgaccgccag gaccgctaca tcggcttcgg ccccgaggag tcggagctcg
      241 cggcgatcgc cgacctcgac acgttcgccc agctcccctg ggactcccgc gtcgcgcgcg
      301 tcttctgcga ctgctacgac accgagaccg gtgagctgct cgaggccgac ccgcgccaga
      361 acctgaagcg gacggtgaac gaggtcgcgc aggagctcgg ctacacgttc ctctgcggga
      421 tcgagcccga gatgatgtgg atgaagcggc cgaaggggtc gaccgacccg ggcgcggccg
      481 agggcatcac caagccctgg tgctaccaca tcaaccagtt cgaggagctg cggccgatca
      541 tcctcgacgt catcgactac ggggagcggc tcggcatcaa cccctcgtac ggcgaccacg
      601 aggactcccc ggggcagctc gagctcaact tcctcttcga ccgcgccgtc aggaccgcgg
      661 acaacctctc gacctatcgc cagctctgcc aggcggccgc ccgcaagcac acgcgggagg
      721 gaacgtacgg cgacgacctc gaggtgctcg caaccttcat gccgaagccg ttcaccggcg
      781 tctcggcaaa cggcgcccat caccacttca cgctcgtgga cgagcagggg cagaacgtct
      841 tctacgacgc cgacggcccc gccaagctgt ccgagctcgg ccgccagttc atgggcggga
      901 tcctcgagca cgcgcgcgct ctctgcggga tcacggcgcc gaccgtgaac tcctacaagc
      961 gcttctggga cttcggcttc tgggcgccga tctacaagga ctacggctgg cagaaccgga
     1021 cgactctggt cagggtcgcg agcggcgggc gcttcgagtt ccgggccgtc gactcgtcct
     1081 gcaacccgga cctgacgcag gcggcgctcc tgcgtgccgg gctcgacggc gtcaagcgcc
     1141 ggctcgaccc cggcccgccc cagcagcgca acatctacga cgtcctcgcc gaggggcagg
     1201 agatcgagcg ggtgcccgag cacctgggcg aggcgctcga cgcgctcgag cgggacgacg
     1261 tcggccggac cgccctgccc ggccgtctct acgatgtgta catgcactac acgcgggacg
     1321 agtgggagcg cttcctgtcg gccgtgaccg actgggagcg cgagcgctac ctggacgtcc
     1381 tcccctagcg agcgcgggag gcggacggcc atgtgcggga tcgccgggat catctaccgc
     1441 gacggcacgc gtccgatcgg acgcgacatg acgcgcatgc tccagtcgat gaagcaccgt
     1501 ggtccggact cgacgggcta tgcgctctac ggggccgagc acgacggacg cgtcgtcatg
     1561 cgctacacgc tggccgacgc gaacaccgcc cgagacttcg atttccacga gcggctcgaa
     1621 cggcaccagc gccaggt
//
LOCUS       DASZOB010000285         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_107319, whole genome shotgun sequence.
ACCESSION   DASZOB010000285 DASZOB010000000
VERSION     DASZOB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107319"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VG709_04670"
     CDS             complement(<1..495)
                     /locus_tag="VG709_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32402.1"
                     /translation="MLALRVLAEVLAAALLGWAIWRYRDRRITRLNLIVSGVLAIGLA
                     LLAGVPALFDPLFDAFQFERGGGMRLIAVLVVGNLILLLLVVSNRGQTDVVRRDLKSL
                     LEWLGAESFDEAQAETLPAGDRIVVVMPAHNEADNIEGVVRAMPEKIDGHVVVPLVVD
                     DASED"
     gene            complement(496..1689)
                     /locus_tag="VG709_04675"
     CDS             complement(496..1689)
                     /locus_tag="VG709_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007303781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC32403.1"
                     /translation="MRVLILGGDGYLGWPTAMRFSSQGHDVYLLDNYLRRRAVREAGS
                     DSLTPIPDDLSTRAEIWKAVSGKEIRVAEGDVADWDTLSTVFREAQPETIVHYGQIPS
                     APYSMVDREHAVFTQYNNVIGNLNVIFAMRDIAPESHLIKLGTMGEYGTPNIDIEEGY
                     IEIEHNGRSDVLPYPKLPGSLYHASKVHDSTNIHFSCRVWGLRATDLNQGVVYGIETE
                     ETKLDERLITRFDYDEIWGTTLNRFCLQAVIGHPLTVYGAGGQTRGFLNIVDTLQCIE
                     LAASNPAAAGEYRVFNQFTEQFSVAELAELVARAGREVGLDVKIDSIENPRVEAEHHY
                     YNAKHTKLLDLGLNPHHLSETLIESMFEVMRRYQDRVITDRILPRVRWAPKAAAEPAP
                     PTTTA"
     gene            1741..3498
                     /locus_tag="VG709_04680"
     CDS             1741..3498
                     /locus_tag="VG709_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC32404.1"
                     /translation="MPARTKRAARVPALRATALASLLWLLAASACVGGRRADEAALEP
                     APSIEAMARAAGSDVVERLASGYVEGRSGEIQLVPEPWSVLGQWNGGVRGPADPRTTH
                     ATPWSYHQRVPIVLYGPPFVRRGVVSDQPASVADAGATLAGLLGLDHVGRSGAPLDDA
                     LAPRGAHRPRAILVVVYDGGGWNVLERWPDAWPFLRRIAGKGATYVNATAGSAPTVTA
                     PVHASLGTGTHPREHGIAENTFRLPSGEIGDALGDRAGDPSLLRRSTFADRWERVTRG
                     RAWVGMVGYDVWHLPMMGAGTPGGDRDAAFLWDRHAAEIGEPWAPSPRYRLPRFAPTV
                     QTLEEELRRLDLVDGAADGRWRDADLANEYEIPGTPAFASFVGEAAVRLVEESPVGRD
                     DVTDLVFVELKASDYAGHLWNMESAAERDVLRTQDRILQRLVRALDRKAGSGRYVVAV
                     TADHGQTPNPASTGGLRIDRYAVAADIEAAFGDVVDEVHPGQVFLDLEALRSSGRTVE
                     DVARFLGAYRYREAIPEGTDVASLPAGALDRRVFAAALPGTFLAGLSSRDVAAFGRGD
                     HPEGDLTTPVRAIEALVRG"
     gene            complement(3488..4189)
                     /locus_tag="VG709_04685"
     CDS             complement(3488..4189)
                     /locus_tag="VG709_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HWC32405.1"
                     /translation="MRRTWSPTRVASGVGLVAWAAAFWFLLVSGRTQLYLGSRTEWVV
                     PLGAVALTLAAAGRLWTARTTHRERLRPRDAWGFAVVALPAVLVLALPPTSLSSFAAS
                     RRSASAGFVSTTGDPSSGDITLVDVASADWSTEGQRALVRRAGEQVSFVGFVTERPGL
                     PADEFYLTRFIVSCCVADALSVQVRVVEAPPGRFQQDQWVRVDGAIYPVAGEVIVAAT
                     NVEPVPEPADPYLNP"
     gene            complement(4186..5229)
                     /locus_tag="VG709_04690"
     CDS             complement(4186..5229)
                     /locus_tag="VG709_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWC32406.1"
                     /translation="MAISQPISVPRARAWRNAVLALAAVVAAAFAIRLLEGVGVGAFQ
                     NFVLVFASLLVEALPFVLLGALVAAAIEVFVPTRFFTRLAAVPRPLQMPVAGLGGFVF
                     PVCECGSVPVTRRLVTKGLTPAAALTFMLAAPILNPIVLVSTAIAYRGRDVVWAMVGG
                     RAVLGLGAAMVVGWILGGRSRTELLKAREEDHVGRACACEHCGGAHEGPPRGARARAR
                     GFFSLASQDFTLLARFLVIGAALASALQTFLPQSFLSAVATTPLVNLVAMMALAAVLS
                     LCSSSDAFVAASFVQFGPAPQLAFLVFGPMVDAKLGFLYAGTFSKGFVRTVIAGVAIV
                     TLVGTLWMQVMIG"
     gene            complement(5232..>5426)
                     /locus_tag="VG709_04695"
     CDS             complement(5232..>5426)
                     /locus_tag="VG709_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32407.1"
                     /translation="VRPDERLIEATAVGGVFVDQSALVRTGRSADAVVRGLRAARASD
                     GRALFADVFPGRAVTFARYC"
BASE COUNT          833 a         1912 c         1947 g          734 t
ORIGIN      
        1 gtcctcggac gcgtcgtcga cgacgagcgg gacgacgacg tggccgtcga tcttctccgg
       61 catcgcgcgg acgacgcctt cgatgttgtc ggcctcgttg tgcgccggca tgacgacgac
      121 gatgcggtct cctgcgggga gcgtctcggc ctgcgcctcg tcgaacgact ccgcgccgag
      181 ccactcgagg agcgacttca ggtcgcggcg cacgacgtcg gtctggccgc ggttcgagac
      241 gacgagcaag aggaggatga ggttgccgac gacgaggacg gcgatcagcc gcatgccgcc
      301 gccgcgctcg aactggaacg cgtcgaacag cgggtcgaag agcgccggca cccccgcgag
      361 cagcgcgagc ccgatcgcga gaacgcctga gacgatcagg ttgagccgcg tgatccggcg
      421 gtcgcggtac cgccagatcg cccagcccag gagggccgcg gcgaggacct ccgccaggac
      481 ccggagcgcg agcacctagg cggtggtggt gggcggcgct ggctcggccg cggccttcgg
      541 cgcccagcgg acgcgcggaa ggatccggtc ggtgattacc cggtcctggt agcggcgcat
      601 gacctcgaac atcgactcga tgagggtctc cgacaggtga tgcgggttca gtcccaggtc
      661 gaggagcttc gtgtgcttgg cgttgtagta gtggtgctcc gcctcgaccc gcgggttctc
      721 gatggagtcg atcttcacgt cgagcccgac ctcacgcccc gcccgcgcga cgagctccgc
      781 gagctccgcg accgagaact gctcggtgaa ctggttgaaa acccggtact cgccggccgc
      841 cgccggattc gacgccgcga gctcgatgca ctggagggtg tcgacgatgt tgaggaagcc
      901 gcgcgtctgg ccgcctgcgc cgtacacggt gagggggtgg ccgatcaccg cctggaggca
      961 gaagcggttg agcgtcgtcc cccagatctc gtcgtagtcg aaccgcgtga tcaggcgctc
     1021 gtcgagcttc gtctcctcgg tctcgatccc gtacacgacg ccctggttca ggtcggtggc
     1081 gcgcagtccc cacacccggc acgagaagtg gatgttcgtg gagtcgtgca ccttcgacgc
     1141 gtggtagagg gatcccggaa gcttcgggta ggggaggacg tccgaacgcc cgttgtgctc
     1201 gatctcgatg tacccctcct cgatgtcgat gttgggggtc ccgtactcgc ccatcgtgcc
     1261 gagcttgatc aggtgcgatt ccggcgcgat gtcgcgcatc gcgaagatca cgttgaggtt
     1321 cccgatcacg ttgttgtact gggtgaagac ggcgtgctca cggtcgacca tcgagtacgg
     1381 cgccgaaggg atttggccgt agtgcacgat ggtttccggc tgcgcctcac ggaacacggt
     1441 cgagagcgtg tcccagtccg ccacatcacc ctcggcgacg cggatctcct tccctgaaac
     1501 ggccttccag atctccgcgc gcgtcgacag gtcgtcgggg atcggcgtga gcgagtccga
     1561 ccccgcctcg cggacggcgc ggcggcgcag gtagttgtcg agcagataca cgtcatggcc
     1621 ctggctggag aaccgcatcg cggtcggcca gcccaggtac ccgtcgcctc caaggatcag
     1681 tacgcgcatc gggcaggttt cctccggtga aagggaatgg gaacgccata ttgtatagcg
     1741 atgccagccc gcaccaagcg ggcggcgcgc gtccccgcgc tccgcgcgac cgcgctcgcg
     1801 tcgctcctgt ggctcctggc cgcttccgcg tgcgtgggag gaaggcgcgc cgacgaggca
     1861 gcgctcgagc ctgctccgtc catcgaggcg atggcgcgcg ccgctgggtc ggacgtcgtc
     1921 gagcggctcg ccagcggcta cgtcgaggga agatcgggag agatccagct cgttcccgag
     1981 ccctggagcg tccttgggca gtggaacggc ggcgtacggg gccccgccga cccgcgcacg
     2041 acgcatgcga cgccgtggtc gtaccaccag cgcgtcccca tcgtcctcta cggacccccg
     2101 ttcgtccgtc gtggggtcgt ctcggaccag ccggcgtcgg tcgccgacgc cggggcgacg
     2161 cttgccgggc tcctcggcct cgatcacgtc ggccgctccg gcgcgccgct cgacgacgcg
     2221 ctggcgccgc gcggcgccca tcgcccgcgc gcgatcctcg tcgtcgtgta cgacggcggc
     2281 gggtggaacg tgctcgagcg gtggccggac gcgtggccgt tcctgcgccg catcgcggga
     2341 aagggtgcga cgtacgtgaa cgcgacggcg ggatccgctc ccaccgtgac cgcgccggtc
     2401 catgcgtcgc tcgggacggg gacgcatcct cgcgagcacg ggatcgccga gaacaccttc
     2461 cggcttcctt ccggtgagat cggcgacgcg ctcggcgatc gtgccggcga cccgtcgctg
     2521 ctccgccgtt cgaccttcgc cgaccgatgg gagcgcgtca cccgaggccg ggcgtgggtc
     2581 gggatggtcg gctacgacgt ctggcacctc ccgatgatgg gggcggggac gccgggagga
     2641 gaccgtgacg ccgcgttcct gtgggaccgt cacgccgctg agatcggcga accatgggcc
     2701 ccctcgccgc ggtaccggct gccgcgcttc gccccgacgg tccagacgct cgaggaggag
     2761 cttcggcgcc tcgacctcgt cgacggcgcg gccgacggcc gctggcgcga cgccgacctc
     2821 gcgaacgaat acgagatccc cgggacgccg gctttcgcct cgttcgtcgg cgaggccgcg
     2881 gtgcgcctcg tcgaggagtc accggtcggc cgcgacgacg tcaccgacct cgtgttcgtc
     2941 gagctgaagg cgtccgacta cgccgggcac ctgtggaaca tggagtcggc cgcggagcgc
     3001 gacgtgctcc gaacccagga ccggatcctg cagcgcctcg tccgcgcgct cgaccggaag
     3061 gccggcagtg ggcgctacgt cgtcgcggtc accgcggacc acggccagac cccgaacccc
     3121 gcatcgacag gtggcttgcg gatcgaccgg tacgctgtgg cggcggacat cgaggcggcg
     3181 ttcggggacg tcgtcgacga ggtgcatccg gggcaggtct tcctcgacct ggaggcgctg
     3241 cgcagctcgg gtcgaaccgt ggaggacgtc gcgcgcttcc tcggcgcgta tcgctaccgc
     3301 gaggcgatcc cggaggggac cgacgtcgcg tcgctccctg ccggtgcgct cgaccgccgg
     3361 gtcttcgcgg ccgctctccc cggaacgttc ctcgccgggc tctcgtcgcg cgacgtcgcg
     3421 gcgttcggtc gcggcgacca tcccgaaggc gatctcacga cgccggtccg tgcgatcgag
     3481 gcactcgtca ggggttgagg tacgggtcgg ccggctcggg cacgggctcg acgttcgtcg
     3541 ccgcgacgat gacctcgccg gcgacgggat agatggcgcc gtcgacacgg acccactggt
     3601 cctgttggaa ccgccccggc ggcgcctcga cgactcgtac ctggacgctc agcgcgtcgg
     3661 cgacgcagca cgagacgatg aaccgcgtga ggtagaactc gtcggctggg agccccgggc
     3721 gctccgtcac gaagccgacg aacgagacct gctcgccggc gcgccggacg agcgcgcgct
     3781 ggccctcggt cgaccagtca gccgaggcga cgtcgacgag cgtgatgtcg cccgaggacg
     3841 ggtcgccggt cgtcgagacg aaccctgcgc tcgccgagcg gcgagacgcg gcgaacgagc
     3901 tcagcgacgt cgggggcagc gcgagcacga ggacggccgg aagcgcgacg acggcgaagc
     3961 cccacgcgtc gcggggacgc agccgctccc gatgggtggt ccgcgccgtc cagagccgtc
     4021 cggccgcggc caacgtgagc gccacggccc cgagcggcac gacccactcg gtgcgcgacc
     4081 cgaggtagag ctgcgtcctc ccggagacga gcaggaacca gaacgccgcg gcccacgcca
     4141 cgagcccgac gcccgatgcg acccgagtcg ggctccacgt ccgtctcatc cgatcatcac
     4201 ctgcatccac agcgtcccga ccagcgtcac gatcgccacg ccggcgatga ccgtccggac
     4261 gaaccccttg ctgaacgtcc ccgcgtagag gaacccgagc ttcgcatcga ccatcgggcc
     4321 gaatacgagg aacgccagct gtggcgcggg gccgaactgg acgaacgagg ccgcgacgaa
     4381 cgcgtcggat gaggagcaaa gcgagagcac cgccgcgagc gccatcatcg cgacgaggtt
     4441 cacgagcggc gtcgtcgcga cagccgacag gaacgactgc ggcaggaagg tctggagcgc
     4501 gctcgcgagc gccgccccga tcacgaggaa ccgcgcgagc agcgtgaagt cctggctcgc
     4561 gagcgagaag aacccgcgcg ctcgggcgcg ggcgcctcgt ggcggccctt cgtgggcgcc
     4621 cccgcagtgc tcgcaggcgc aggcccggcc gacgtggtcc tcctcgcgcg ccttcagcag
     4681 ctccgtacgg ctgcggccgc cgaggatcca ccccacgacc atcgctgccc cgaggccgag
     4741 gaccgcgcgg ccgccgacca tcgcccagac gacgtcgcgc ccccgatacg cgatcgcggt
     4801 cgagacgagg acgatcgggt tgaggatcgg cgcggcgagc atgaacgtca gcgccgctgc
     4861 gggggtgagt cccttggtca cgaggcgacg cgtgacgggc accgatccgc actcgcatac
     4921 ggggaaaacg aaccccccga gcccggcgac cggcatctgg agcgggcggg ggaccgccgc
     4981 cagcctcgtg aagaagcggg tcggcacgaa tacctcgatc gccgccgcga ccagggcccc
     5041 gagcagcacg aacggaagcg cctcgacgag cagcgaggcg aagaccagca cgaagttctg
     5101 aaaggcgccg accccgacgc cctcgagcag ccggatcgcg aacgccgctg caacgacggc
     5161 cgcgagcgcc aggaccgcgt tccgccacgc ccgtgcgcgc ggaaccgaga tcggctgtga
     5221 gatcgccacg tctagcagta tctcgcgaac gtcacggccc gaccggggaa gacgtcggcg
     5281 aagagcgccc ggccgtcgga cgcgcgcgcg gcgcgcaacc cgcgcacgac ggcgtcggcg
     5341 ctccggccgg tccggacgag cgcggactgg tcgacgaaga caccgcccac ggcggtggcc
     5401 tcgatcagcc gctcgtccgg ccgaac
//
LOCUS       DASZOB010000286         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_134142, whole genome shotgun sequence.
ACCESSION   DASZOB010000286 DASZOB010000000
VERSION     DASZOB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134142"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..222)
                     /locus_tag="VG709_04700"
     CDS             complement(34..222)
                     /locus_tag="VG709_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32408.1"
                     /translation="MDATRLGQTALFGWREKVADAVAKRAPIRSEQARAAIGAVFFAL
                     ALRYVIQTARRAARETRS"
     gene            complement(265..>2069)
                     /locus_tag="VG709_04705"
     CDS             complement(265..>2069)
                     /locus_tag="VG709_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWC32409.1"
                     /translation="ARLDDCISARERAFTGYLEDGNLRRAAIVAIALAKDYYAKGSSS
                     IGSAWVKRAERILADTPVCVEQGWLHRIQGVIALEGRGDYDAALHEARRSHELAARFG
                     DTDLAAVSLHDEGRALVMQGDVAGGMALIDEATAPAISGELTPYNTGVIYCNTITACK
                     DLADYRRAGDWTEAAKRWCDRQAIAGFPGMCRVYRASIMMVRGAWAEAEVEARQACEE
                     LGAFNLSYAAEAFYELGEIRMRAGDHGAAEEAFTQAHELGREPQPGLALLRLAQGRVE
                     SASSCIEEALEHESGALHRSRLLPAQVAVALAADDRATARTATEECEAIATRFGSDAL
                     KAAAAATRAQLALAENDTRAAARASRHALRLWQAIDAPYEAANARLLLGEAYLVRGDG
                     DDAALEFEAARSVFARLGAALDARRAREPLDGARRAAGRGSGGPAKRTFMFTDVVRST
                     DLVAAIGDDAWTDVVGWHARTLRTLFEEHGGEEIDHAGDGFFVAFEDPRRAVESAVAI
                     QRRLAEHRRQHGFAPQVRIGLHASEAARDRGAYRGRGVHEAARISALAGADEIVASND
                     TVRANPPRFPVSTPRTVELKGIPAPVEVVSVDWR"
BASE COUNT          277 a          751 c          733 g          308 t
ORIGIN      
        1 gccttgcgtc cggccgcgag cctgacgcgc gcgtcaggat cgggtctcgc gcgcggctcg
       61 cctggccgtc tggatcacgt agcggagggc caacgcgaaa aagaccgcgc cgatggcggc
      121 gcgcgcctgc tcggaccgga tcggcgcgcg cttcgccacc gcgtctgcga ccttttcgcg
      181 ccacccgaag agcgccgtct gtccgagccg tgtcgcgtcc atcgggagcg aactacccgg
      241 ccgccggccg cgcaaacgtc gttgctaccg ccagtcgacg gagacgacct cgacgggggc
      301 cggaattccc ttgagctcca cagtccgcgg agtcgacacc ggaaaccgcg gcggattcgc
      361 gcgcacggtg tcgttgctcg cgacgatctc gtccgcgccg gcgagtgctg agatgcgtgc
      421 ggcctcgtgc acgccgcggc cgcggtacgc gcccctgtcg cgggcggcct cgctcgcgtg
      481 gaggccgatt cgtacctgcg gcgcgaagcc gtgctgacgt cgatgctcgg cgaggcgccg
      541 ctggatcgcg acggcggact cgaccgccct tcggggatcc tcgaaggcga cgaagaagcc
      601 gtcgcccgcg tggtcgatct cctcgccccc gtgctcttcg aacagggtcc gcagcgtccg
      661 ggcgtgccac ccgacgacgt cggtccaggc gtcgtcgccg atcgcggcca cgaggtccgt
      721 cgagcgaacg acgtccgtga acatgaacgt ccgtttcgcc ggtccgccgc tccctctacc
      781 cgccgcgcgc cgagcgccgt cgaggggctc gcgtgcgcgg cgtgcgtcga gcgccgcgcc
      841 gaggcgcgcg aacaccgaac gtgcggcctc gaactcgagc gcggcgtcgt cgccgtctcc
      901 gcgcacgagg taggcctcgc cgagcagtag gcgggcgttc gccgcctcgt agggagcgtc
      961 gatcgcttgc cagagccgaa gcgcgtggcg agacgcgcga gccgcggccc gcgtgtcgtt
     1021 ctcggcgagt gcgagctgcg ctcgcgtggc tgcggcggcc gccttcagcg cgtcgcttcc
     1081 gaaccgggtg gcgatcgcct cgcattcctc ggtcgcggta cgagcggtcg cgcggtcgtc
     1141 ggccgcgagc gcgacggcaa cctgggcagg aagcaggcgg gaacgatgga gggcgcccga
     1201 ctcgtgctcg agcgcctcct cgatgcacga cgaagcgctc tcgactcgtc cctgagcgag
     1261 acgcaggagc gcaaggcccg gttgtggctc gcgccccagc tcgtgcgcct gcgtgaacgc
     1321 ctcctcggcg gcgccgtggt cgcccgcgcg catgcgtatc tcgcccagct cgtagaacgc
     1381 ctcggccgcg tagctcaggt tgaacgcgcc gagctcctcg cacgcctgac gcgcttccac
     1441 ctccgcctcg gcccacgccc cgcgcaccat catgatcgaa gcccggtaca cccggcacat
     1501 cccggggaac cccgcgatcg cctggcggtc acaccagcgc ttcgctgcct cggtccagtc
     1561 gcccgctcgg cgatagtcgg cgaggtcctt gcaggccgta atcgtgttgc agtagatgac
     1621 gcccgtgttg taaggcgtga gctctccgga gatcgcgggg gcggtcgcct cgtcgatcag
     1681 agccatgccg ccggcgacat cgccctgcat cacgagcgcg cgtccctcgt cgtggagcga
     1741 gacggcggcg aggtcggtat cgccgaaccg cgccgcgagc tcgtggctcc ggcgagcctc
     1801 gtggagcgcg gcgtcgtagt cgccgcgccc ttccagcgcg atcacaccct ggatacggtg
     1861 gagccatcct tgctcgacgc agacgggagt gtcggcgagg atgcgctcgg cccgcttcac
     1921 ccaggcggat ccgatcgacg acgagccctt ggcgtagtaa tcctttgcaa gcgcgatggc
     1981 gacgattgcg gcacgacgga ggtttccgtc ctcgaggtag ccggtgaagg cgcgctctcg
     2041 ggccgagatg cagtcgtcga gccgcgcgg
//
LOCUS       DASZOB010000287         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_167325, whole genome shotgun sequence.
ACCESSION   DASZOB010000287 DASZOB010000000
VERSION     DASZOB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167325"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1428)
                     /locus_tag="VG709_04710"
     CDS             complement(187..1428)
                     /locus_tag="VG709_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HWC32410.1"
                     /translation="MQLSRSFVVLFAVLVLALPATAAPGDLDTTFSADGMVLVDNDVG
                     DEAGGLALQGDGKILQAGETGLPGVDDFMVVRYTRDGVPDSTFGGDGIVTTDFFGADD
                     YATGVISLPGNKTLAVGYGRPSGGVYEFALAQYNANGTPDGSFGGDGKVTTPFPGHGY
                     AYAALRLTDGSIVVVGERYEGHGDFALAKYRPNGALDTSFSGDGKRTLDFAGGSDGAV
                     DIVAAGDRLLLSGWGMDGPSPNDYDIALARFKRGGALDRQFSGDGKRLLSLGAGSQDY
                     ADGLVRLGDGRFVVAAGLGGGDVALAKFKPGGGLDGTFGGGDGKALVDFGANETVSGL
                     ARVARGYAVGVRQSNQMGGARFGPRGSPIGSFGTGGLALAAFANQSFVTGVANQDGKM
                     VTGGFAFTGTSNDFAVARFLT"
     gene            complement(1522..2277)
                     /locus_tag="VG709_04715"
     CDS             complement(1522..2277)
                     /locus_tag="VG709_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32411.1"
                     /translation="MPTAPRCSATAPSSRSARSTGGSPVADMAIAKYRAAGRLDREFG
                     RRGKRTIDFAGGEDGAEAVAFQDGKILVGGWGQQASGPSDDDVALARLHPSGALDRGF
                     GRRGKRLLGNLNGNQWVEGLSVLRSGRFVIALYADEDFALARFRANGTLDDDFGAGGF
                     AIRNFGGAEYTKDLVRAGNRLYVIGIAGDDFAVAAFGASGRLLSGFGTGGLAIADFGG
                     NDYAYAATLVGGRVVVAGLVDNQHFGLARFLVQ"
BASE COUNT          367 a          862 c          734 g          325 t
ORIGIN      
        1 cccggaggcc gagcggaagc agcgtgtaca cgccggccat cagcttccgg acgaacgcgc
       61 cgcggatcag gaggcgcgcg gaaggcgcct cctcgtcggc cggcgcctcc cgcagcgtgc
      121 gcgcgaacag ctcgctggct cgcatggtcc agccaggata gccgctgtcc gctcaggccc
      181 cggtcgctac gtcaggaacc gagcgaccgc gaagtcgttg ctcgtgccgg tgaacgcgaa
      241 gccccccgtc accatcttgc cgtcctggtt cgccaccccg gtcacgaacg actggttcgc
      301 aaaggctgcc agggccagcc cgcccgttcc gaacgagccg atggggctcc cacgcggccc
      361 gaaccgggcc ccgcccatct gattcgactg acgaacgccg acggcgtagc cccgcgcgac
      421 gcgagcgagg cccgagaccg tctcgttcgc cccgaagtcg accagggcct tgccgtctcc
      481 gccgccgaag gtgccgtcga gcccgccccc gggcttgaac ttcgccagcg ccacatcgcc
      541 gccgcccaag ccggccgcga cgacgaagcg cccgtcgccg agacggacca gaccgtcggc
      601 gtagtcctgc gaacctgcgc ccagcgagag cagccgtttg ccgtcgccgc tgaactgccg
      661 gtccagcgcg ccacccctct tgaaccgcgc cagcgcgatg tcgtagtcgt tgggggacgg
      721 cccgtccatg ccccatcccg agagcaggag gcgatcgccc gcggcgacga tgtccacagc
      781 gccgtcggag ccgccggcga agtccagtgt cctcttccca tcgccgctga acgaggtgtc
      841 gagggctccg ttcggccggt acttcgccag cgcgaaatct ccgtgcccct catagcgctc
      901 cccgaccacg acgatcgagc cgtcggtgag acgcagagcc gcgtaggcgt aaccgtggcc
      961 cgggaacggc gtcgtcacct tcccgtcccc gccgaacgag ccgtcggggg tcccattcgc
     1021 gttgtactga gcgagcgcga actcgtaaac gccgccggac ggccggccgt agccgaccgc
     1081 cagggtcttg ttccccggaa gggagataac cccggtggcg taatcgtccg cgccgaagaa
     1141 gtctgtcgtg acgatgccgt cgccaccaaa tgtggagtcg ggaacgccgt ccctcgtgta
     1201 ccggaccacc atgaaatcgt ccacgcccgg taggccggtc tctccggcct ggaggatctt
     1261 cccgtccccc tgcagggcga ggccgccggc ctcgtcgccg acgtcgttgt cgaccaagac
     1321 catcccgtcc gcggagaacg tcgtgtcgag gtcgcccgga gcggccgttg ccggcagcgc
     1381 gaggaccagg acggcgaaca agacgacgaa cgaccgtgag agctgcatgg ttaccccttc
     1441 ccgtgggccg caagaggccc gatcagacac gtgaacgatc ctcttccggc catcccgcgt
     1501 gtcgtgtcgt atgtcatcgc gctactgcac gagaaaccga gcgaggccga agtgctggtt
     1561 gtcgaccagc ccggcgacga cgacccgccc gccgacgagc gtcgccgcgt acgcgtagtc
     1621 gttgccgccg aagtccgcga tcgccagacc gccggttccg aaccccgata ggagccttcc
     1681 gctcgcaccg aacgccgcga cggcgaagtc gtcgcccgcg ataccgatca cgtacaggcg
     1741 gttcccggct cggacgaggt ctttcgtgta ctcggcgcca ccgaagttcc tgatcgcgaa
     1801 gcctcccgca ccgaagtcgt cgtcgagggt tccattcgca cggaagcgag ccagtgcgaa
     1861 gtcctcgtcg gcatagagcg cgatcacgaa cctgccgctg cgaagcaccg agagaccctc
     1921 gacccactgg ttgccgttga ggtttccaag cagcctcttg cctcgccttc cgaagccgcg
     1981 gtccagcgct ccgctcgggt gcaggcgcgc aagcgcgacg tcgtcgtccg aagggccaga
     2041 agcctgctgt ccccacccgc cgacgaggat cttcccgtcc tggaaggcca ccgcctcggc
     2101 gccgtcctcg cctccggcga agtcgatcgt gcgcttcccc ctccgcccga actcgcggtc
     2161 gagccgtccg gcggcgcggt acttggcgat cgccatgtcg gcgaccggcg acccccccgt
     2221 agacctcgcc gaccgcgacg aaggagccgt cgcgctgcac cgcggcgccg taggcagcgg
     2281 cctggagg
//
LOCUS       DASZOB010000288         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_198205, whole genome shotgun sequence.
ACCESSION   DASZOB010000288 DASZOB010000000
VERSION     DASZOB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198205"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VG709_04720"
     CDS             complement(<1..147)
                     /locus_tag="VG709_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWC32412.1"
                     /translation="MRCPWCGHPETKVVDSRPAEEGAAIRRRRACESCDRRFTTFERS
                     DTLGV"
     gene            224..910
                     /locus_tag="VG709_04725"
     CDS             224..910
                     /locus_tag="VG709_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWC32413.1"
                     /translation="MPNELELSLRLVLAAALGGAIGIEREISDQPAGFRTHMLVSLGA
                     CLFAVVSAYGFDAFLEAGLAEQVRFDPSRLAAQIVTGIGFLGAGAIIRYGMTVRGLTT
                     AASLWVVAAIGTAVGIGTYIVSLVTTVITLISLYGLKRVRGRLVRGLKKEHEEFLIEA
                     GRDVALDGVLAALSDAAIRVDHVRVEDERGEDARQIVLFLAMPPGRDPEEVAALLSSM
                     EGIRGVDWTR"
     gene            complement(915..1199)
                     /locus_tag="VG709_04730"
     CDS             complement(915..1199)
                     /locus_tag="VG709_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWC32414.1"
                     /translation="MNRTRVRWPVVILVSLALSLGFLAGRAFGDAGPGREPAAATYVV
                     RSGDTLWRIARGRVGEAGDPRPLIARIVQVNGLASSALQPGTELVVPSPP"
     gene            1290..1907
                     /gene="lexA"
                     /locus_tag="VG709_04735"
     CDS             1290..1907
                     /gene="lexA"
                     /locus_tag="VG709_04735"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWC32415.1"
                     /translation="MDEITPRQQRILDVIRRTVRERGYPPTVREIGEAVGLTSSSSVH
                     AQLENLRRRGMLRKDATKPRAIEIAGRTAAEPEAAAVPLVGRIAAGAPVLAEEHVEEY
                     LAVPLDFAARGEHFALRVAGDSMQGAGILEGDTVVVRQQDVAEDGAIVAALLPGAGED
                     EATVKRLRRRQGRPVLEPENPAHDAFVLPEGGRILGQVVAVLRKV"
     gene            complement(1911..>2546)
                     /locus_tag="VG709_04740"
     CDS             complement(1911..>2546)
                     /locus_tag="VG709_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWC32416.1"
                     /translation="LTSADVLVADQLFATLDPTVRRLRLPGGRVVTISDTVGFVRKLP
                     HDLVEAFRSTLEEVTLGSLVLHVADASAKDVEEQVDAVRATLAEIGAGGVPEVLALNK
                     MDLLSEVERARMQRVFPDGVPVSALQSEGVEELLTRVEMALPVRSVEVELLVPYERHD
                     VVARLHEQAEIVSSESSNEGTMLRARVNEEQVPTLQEFLVRPVTQRVRVRG"
BASE COUNT          379 a          918 c          877 g          372 t
ORIGIN      
        1 gacgccgagc gtgtcggacc gctcgaaggt cgtgaatcgg cggtcgcacg attcgcatgc
       61 ccgccgacgg cggatcgccg ctccctcctc ggccgggcgc gagtccacaa ccttggtctc
      121 cggatgtccg caccatggac agcgcatcgg ttctctccac atcttggcgc gcaccgcgcg
      181 cgaccacaac atatggggcg tcgaggacgg ggataatccg tacgtgccga acgagctaga
      241 gctctcgctg cggctcgtcc tcgcggccgc gctcggcggg gccatcggga tcgagcgcga
      301 gatctcggac cagccggcgg ggttccggac gcacatgctg gtctcgctcg gcgcgtgcct
      361 tttcgccgtc gtgtccgcgt acggcttcga cgcgttcctc gaagccggcc tcgcggagca
      421 ggtccgcttc gacccctcac gcctcgcggc ccagatcgtg accgggatcg gcttcctcgg
      481 ggcgggcgcg atcatccggt acggcatgac ggtccggggc ctcacgacgg cggcaagcct
      541 ttgggtcgtc gcggcgatcg gcaccgccgt cgggatcggg acctacatcg tctcgctcgt
      601 cacgacggtc atcacgctga tctcgctcta cggcctgaag cgcgtccgcg gccggctggt
      661 ccgcggcctc aagaaggagc acgaggagtt cctcatcgag gccgggcgtg acgtcgcgct
      721 cgacggggtc ctggccgcgc tctcggatgc cgcgatccgc gtcgaccacg tccgcgtcga
      781 ggacgagcgg ggtgaggacg cgaggcagat cgtgctgttc ctcgcgatgc cgcccgggcg
      841 cgatcccgaa gaggtggccg ctctcctgtc gagcatggaa ggcatccgcg gcgtcgactg
      901 gacgcggtag gcggctaggg cggcgacggg accactagtt ccgtcccggg ctggagcgcc
      961 gagctcgcga gcccgttgac ctgcacgatg cgcgcgatca gtggcctcgg gtctccggcc
     1021 tcgccaacgc ggccccgcgc gatcctccac aacgtgtcgc cggatcgcac gacgtaggtc
     1081 gccgcggccg gctcgcggcc cgggccggcg tccccgaacg ctcgccccgc gaggaatccc
     1141 agcgagagcg ccagcgagac caggatcacg accggccatc gaacgcgtgt tcgattcacg
     1201 cgcccagtat accgaacgcc tgttcgactt ccaggactcg aacgtatgtt tgattccatg
     1261 gcgatcgcat gctagcgtcc ccctctgaca tggacgagat cacgccgcgg caacagcgga
     1321 tcctcgacgt catccgccga acggtccgcg agcgcggcta cccgccgacc gtccgcgaga
     1381 tcggcgaggc cgtggggcta acctcgtcgt cgagcgtcca cgcccagctc gagaacctcc
     1441 ggcgcagggg aatgctccgg aaggacgcca cgaagccgcg cgcgatcgag atcgccggcc
     1501 gtaccgcggc cgagccggag gccgccgcgg tccccctcgt gggcaggatc gccgccgggg
     1561 cgccggtcct cgcggaggag cacgtcgagg agtacctcgc ggtcccgctc gacttcgcgg
     1621 ctcgcgggga gcacttcgcg ctgcgcgtgg cgggcgactc gatgcaggga gcggggatcc
     1681 tcgagggcga cacggtcgtc gtccgccagc aagacgtcgc ggaggacggc gcgatcgtcg
     1741 cagcgctcct tcccggcgcc ggcgaggacg aagcgaccgt caagcgcctc aggcgccgcc
     1801 agggacgtcc tgtcctcgag ccggagaacc ccgcgcacga cgcgttcgtc ctgcccgagg
     1861 gcggccggat cctcggccag gtcgtcgctg tcctgcggaa ggtctgacgc tcagccgcgg
     1921 acgcgtaccc gctgcgtgac cggtcggacc aggaactcct ggagggtcgg cacctgctcc
     1981 tcgttcacgc gtgcgcggag catggtgccc tcgttcgagc tctcgctcga cacgatctcg
     2041 gcctgctcgt ggaggcgcgc gaccacatcg tggcgctcgt acggcacgag gagctcgacc
     2101 tcgacggacc gcaccgggag cgccatctcg acccgcgtca gcagctcctc aaccccttcc
     2161 gactggagcg ccgacaccgg cacgccgtcc gggaatacgc gctgcatgcg cgcacgctcg
     2221 acctccgaga gcaggtccat cttgttgagc gccagcacct cgggaacccc accggcaccg
     2281 atctcggcga gcgtggcgcg aacagcatcg acctgttcct cgacgtcctt cgcggacgcg
     2341 tcggcgacat ggagcacgag cgatccgagc gtgacctcct cgagcgtcga gcggaatgcc
     2401 tcgacgaggt cgtgcggcag cttccgcacg aagccgacgg tatccgagat cgtgaccacc
     2461 ctcccgccgg ggagccgcag tcgcctcacg gtcgggtcga gcgtcgcaaa cagctgatcg
     2521 gcgacgagga cgtcggcgct cgtgag
//
LOCUS       DASZOB010000289         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_147353, whole genome shotgun sequence.
ACCESSION   DASZOB010000289 DASZOB010000000
VERSION     DASZOB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147353"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1146
                     /locus_tag="VG709_04745"
     CDS             106..1146
                     /locus_tag="VG709_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWC32417.1"
                     /translation="MAVREAAPVGSLHEIREAGLDREDLLGVYRHLILTRGIEDRGHI
                     LYKQGKIPGSFYTGRGNEGASVGVAAAMGPKDVGTPLHRDMGVHVVRGVEPWRIFAQY
                     MGRVDGPTGGRDGNVHMADDRLGLIAMVSHLPAMLPVAVGCALAFRIREEQRVAVGWF
                     GEGASARGDCHEAMNFAGTRRLPVVFVCDNNQWAYSTPTHLEYACETLADRAQAYGFD
                     GVVVDGTDVLAVYREAKRSIEKAREGGGPTLIECLTLRMEGHAVHDDASYVPKEMFEE
                     WAKRDPLERFRTWLRENADMTDEEEDEIGVEIKKLLNDAIRRAEESPLPDAATVTEGV
                     YASPEDLDTPHH"
     gene            1190..>2119
                     /locus_tag="VG709_04750"
     CDS             1190..>2119
                     /locus_tag="VG709_04750"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWC32418.1"
                     /translation="MAVKTYLQAISDGLRQEMRRDKRVYVIGEDVGVYGGAFKVTQGF
                     QDEFGPWRVIDAPLSETAIVGSCTGAAIMGLRPVAEMQFADFVTCAYDQLHTVAAKQH
                     YRAGTAIPIVVRCPSGGGFSGGPFHSQNPESTFAHIAGLKVACPATPADAKGLIVSSI
                     EDPNPVLFFEHKHLYRRIKDEVPDERYTVPFGEARVHREGDDVTVVTWGAMVYTADEA
                     AKKLEDEDVSVEIVDLRTVLPWDKKRVLASVEKTSKVLVLHEDTRTGGFGAEIAATIA
                     EEAFEDLDAPIKRIAAPDTPVPFSPPLEKAFIPQ"
BASE COUNT          358 a          728 c          749 g          284 t
ORIGIN      
        1 cgcacatggt gcccgacgga gccgaagcgc ggggccgcga ccggttcgtc ggactctgcg
       61 caagcgggta gagtgcgacc cgctcacgtc gaagggggaa cgcacatggc ggtccgggaa
      121 gcagccccgg tcggctcgct ccacgagatc agggaagccg gcctcgatcg cgaggatctc
      181 ctcggcgtct accggcacct catcctgacc cgcgggatcg aggatcgggg gcacatcctg
      241 tacaagcagg gcaagatccc cgggagcttc tacaccggcc gcgggaacga gggcgcgtcc
      301 gtcggcgtcg cggcggcgat ggggccgaaa gacgtcggca ccccgctcca ccgcgacatg
      361 ggcgtccacg tcgtccgcgg cgtcgagccg tggcgaatct tcgcgcagta catgggccgg
      421 gtggacgggc cgaccggcgg ccgggacggg aacgtccaca tggccgacga ccgcctcgga
      481 ctgatcgcga tggtcagtca tcttccggcc atgctgcccg tggccgtcgg ctgcgcgctc
      541 gcctttcgca tccgcgagga gcagcgcgtc gcggtcggat ggttcggcga aggcgcctcc
      601 gcgcgcggcg actgccacga ggcgatgaac ttcgccggca cacggcggct tccggtcgtg
      661 ttcgtctgcg acaacaacca gtgggcgtac tcgacgccga cgcacctcga gtacgcgtgc
      721 gagacgctcg cggaccgcgc gcaggcgtac ggcttcgacg gagtcgtggt cgacggcacg
      781 gacgtcctcg ccgtctaccg cgaggcgaag cgctcgatcg agaaggcgcg cgaagggggc
      841 ggcccgacgc tgatcgagtg cctcacgctc cggatggagg ggcacgcggt ccacgacgac
      901 gcgtcctacg tgccgaagga gatgttcgag gagtgggcga agcgcgaccc gctcgagcgc
      961 ttccggacgt ggctccgcga gaacgccgac atgacggacg aggaagagga cgagatcggc
     1021 gtcgagatca agaagctcct gaacgacgcg atccggcgcg cggaggaaag cccgcttccc
     1081 gacgccgcaa cggtcaccga aggcgtctac gcctcgcccg aggacctcga cacgccgcac
     1141 cactgacgac agtgcccatc cacctcagca gctgacccag gaccggccca tggcagtcaa
     1201 gacctacctc caggcgatct ccgacgggct ccggcaggag atgcggcgcg acaagcgcgt
     1261 ctacgtgatc ggcgaggacg tcggcgtcta cggcggcgcg ttcaaggtga cgcaggggtt
     1321 ccaggacgag ttcgggccct ggcgcgtgat cgacgcgccg ctctccgaga ccgcgatcgt
     1381 cgggagctgc actggggcgg cgatcatggg gctgcgcccg gtcgccgaga tgcagttcgc
     1441 cgacttcgtc acgtgcgcgt acgaccagct ccacacggtc gccgccaagc agcactaccg
     1501 cgcgggcacg gcgatcccga tcgtcgtccg ctgcccctcg ggcggcggct tctccggcgg
     1561 ccccttccac tcgcaaaacc ccgagtcgac gttcgcgcac atcgccgggc tgaaggtcgc
     1621 gtgcccagcg acgccggcgg acgcgaaggg gctcatcgtg agctcgatcg aggacccgaa
     1681 cccggtcctc ttcttcgagc acaagcacct gtaccggcgg atcaaggacg aggtgccgga
     1741 cgagcgctac accgttccgt tcggcgaggc gcgcgtccac cgggaaggtg atgacgtgac
     1801 cgtggtcacg tggggcgcga tggtctacac ggccgacgag gcggccaaga agctcgagga
     1861 cgaggacgtc tccgtcgaga tcgtcgatct ccgtaccgtc ctgccctggg acaagaagcg
     1921 ggtgctcgcc tcggtcgaga agacgtcgaa ggtgctcgtc ctccacgagg acacgcgcac
     1981 cggcggcttc ggcgccgaga tcgcggcgac gatcgccgag gaggcgttcg aggacctgga
     2041 tgcgccgatc aagcgcatcg ccgcgcccga cacgcccgtc ccgttctcgc cgccgctcga
     2101 gaaggcgttc atcccgcag
//
LOCUS       DASZOB010000290         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_67091, whole genome shotgun sequence.
ACCESSION   DASZOB010000290 DASZOB010000000
VERSION     DASZOB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67091"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VG709_04755"
     CDS             complement(<1..492)
                     /locus_tag="VG709_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWC32419.1"
                     /translation="MARTHRSALDAVGEKVEANERLDFDDGLTIMESDAVLRLGELAD
                     TARRRRGGSDDVYFVQNLYLNQTNVCRVKCKFCAFARTQKQDGAYTFTPDELVEDALR
                     QRELTGFTEIHMVGGESPHVDLAYYVDIVRKLKDAMADVHLKCFTASEIHHMTKLSGL
                     SHED"
     gene            480..1544
                     /locus_tag="VG709_04760"
     CDS             480..1544
                     /locus_tag="VG709_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HWC32420.1"
                     /translation="MSAPPSPSCLPVDHEPGQRLEGLAVRLADTARRLRGGSDDAYFV
                     QNLYLNQTNVCRVKCKFCAFARTQKQDGAYTFTPEELVEDALRQRDLTGFTEIHMVGG
                     ESPHVDFAYYVDIVMKLKDAMPDVHLKCFTASEIHHMTTLSGLSHEEVLHELRDAGLG
                     SLPGGGAEIFADRVRRLVAPGKEHPDFWFRTHRAAHALGIPTHCTMLYGHVETYEERI
                     DHLLRLRELQDETGGFLAFIPLAFHPENTVFERRGWKHTAGTDDLKVIAVSRLLLDNV
                     PHVKAYWIMMGMPLAAVALHFGANDVQGTVVREHIFHAAGARTETEQKVEELVRFVAE
                     AGRVPVQRDTLYNQLRRWDP"
BASE COUNT          276 a          640 c          607 g          299 t
ORIGIN      
        1 gtcctcgtgc gagaggcccg agagcttcgt catgtggtgg atctcgctcg ccgtgaagca
       61 cttgaggtgg acgtcggcca tcgcgtcctt gagcttccgg acgatgtcca cgtagtaggc
      121 gaggtcgacg tgcgggcttt cgccgccgac catgtggatc tcggtgaacc cggtgagctc
      181 gcgctggcgc agtgcgtcct cgacgagctc gtccggggtg aaagtgtacg cgccgtcctg
      241 cttctgcgtc cgcgcgaacg cgcagaactt gcacttcacg cggcacacgt tcgtctggtt
      301 caggtacagg ttctggacga agtagacgtc gtccgagccg ccgcggcgcc tccgcgccgt
      361 gtccgcgagc tcgccgagcc ggagaacggc gtccgactcc atgatcgtga gcccgtcgtc
      421 gaagtcgagg cgctcgttgg cctccacctt ctccccgacg gcgtcgaggg ccgagcggtg
      481 tgtccgcgcc accgtcgcca tcatgtctcc cggtcgacca cgagcctggt cagcgcctcg
      541 agggactcgc ggtccggctc gccgacacgg cgcggcggct ccgcggcggc tcggacgatg
      601 cctacttcgt ccagaacctg tatctcaacc agacgaacgt ctgccgcgtg aagtgcaagt
      661 tctgcgcctt cgcacgcacg cagaagcagg acggcgccta cacgttcacg cccgaggagc
      721 tggtcgagga cgcgctccgc cagcgggacc tgacgggctt caccgagatc cacatggtcg
      781 gcggcgagag cccgcacgtc gacttcgcct actacgtcga catcgtcatg aagctgaagg
      841 acgcgatgcc cgacgtgcac ctcaagtgct tcaccgcgag cgagatccac cacatgacga
      901 cgctctccgg gctctcgcac gaggaggtgc tccacgagct ccgcgatgcc gggctcggct
      961 cgctcccggg cggcggcgcc gagatcttcg ccgaccgcgt gcgccgcctc gtcgcgcccg
     1021 ggaaggagca tcccgacttc tggtttcgca cgcatcgagc cgcgcacgcg ctcggcatcc
     1081 cgacgcactg cacgatgctg tacggccacg tcgagacgta cgaggagcgg atcgatcacc
     1141 tgctccgcct acgcgagctc caggacgaga ccggtggttt ccttgcgttc atcccgctcg
     1201 ccttccatcc ggagaacacg gtcttcgagc ggcgcggctg gaagcacacc gccggcaccg
     1261 acgacctgaa ggtgatcgcg gtgtcgcggc tcctcctcga caacgtcccg cacgtgaagg
     1321 cctactggat catgatgggg atgccgctcg ccgcggtcgc gctccatttc ggcgcgaacg
     1381 acgtccaggg cacggtcgtg cgggagcaca tcttccatgc ggccggcgcg cgcaccgaga
     1441 cggagcagaa ggtggaggag ctggttcgct tcgtcgcgga ggcgggccgc gtgcccgtcc
     1501 agcgcgacac cctctacaac cagctgcgcc ggtgggatcc ttgacacgct ttcggcacgc
     1561 cgcggcacac acgcgcgcgg ttctcggcgc taggctcagc ccgagcaggg gggcgggtcg
     1621 ggacccggat tatcacgcgc gcgtcattca caccgggggc gcgcggtacc tcgcgagcgt
     1681 gcggcggtga tccggctcgg acgcatctcg tacgtcaaca tggcgccgct cttctttcgg
     1741 ctcgacgccg ccgtcgccga ggtgcccggc gttcccacgg agctcaaccg ggcgctcctc
     1801 gcaggggagg tcgacctggc ac
//
LOCUS       DASZOB010000291         7497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_86935, whole genome shotgun sequence.
ACCESSION   DASZOB010000291 DASZOB010000000
VERSION     DASZOB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86935"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VG709_04765"
     CDS             complement(<1..359)
                     /locus_tag="VG709_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC32421.1"
                     /translation="MPEVVPIGARWVGRAFLVEHRGVVTLVDTGTWMSERRIAAAFER
                     MGRRPTDVRQIVISHSHGDHVGGAASLRETCQAPVSVGAADAPVIEGREPYAYAPATW
                     ARASYSWLDRFPAVPSRP"
     gene            complement(363..1190)
                     /locus_tag="VG709_04770"
     CDS             complement(363..1190)
                     /locus_tag="VG709_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWC32422.1"
                     /translation="MRTLWRGAISFGLVSIPVRLYTATEEKSLHFNQLHEKDHGRIRY
                     KRWCTVEDKEVPYDEIVRGYEYEKDRYVILDDEDLDAVPVESTRTIDIQQFVDLEEID
                     PIYFKKTYYLGPEETGVKAYRLLSEGLARGSKVGVAKVSFRDKEHLATIRLRDGVLVL
                     ETMYWPDEIREAEFPELDKDVKVRDQEIQMAQSLIDNLTEDWKPDRYTDEYREALLDI
                     VQRKVEGEEVEIVEPPEPTKVSDLMEALKRSVEDTRKEAAKETGKKRKAPARKRAAS"
     gene            1284..3797
                     /gene="ligD"
                     /locus_tag="VG709_04775"
     CDS             1284..3797
                     /gene="ligD"
                     /locus_tag="VG709_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWC32423.1"
                     /translation="MPRKKRTGPAFTVETPEPLPASREGEAWWLERDGRRVRLSNLDK
                     VFWPEEGYTKGDLLAYYYNVAELILPYVRERPLTMRRMPDGMAGHEFYEKRSPPNTPE
                     WMSKCVVPADDEGERDIDYLLAEDVPSLLFVVNMGAIEFHPLHSRCGSIDRPDYLFFD
                     LDPFPPATFDDVLAVARHVRAALDALGLPSYPKTSGATGMQVYVGLDPQPTYDDVRGF
                     VRALCRHINAADPDRTTMEWDISKRAGKVFLDWNMNRLGANIAAAYSVRPEPGATVST
                     PLSWDEVEAGVTPRDCTIANAHERFADVGDLFRGMLEEPIDIRPAMDSMGVKPDGETS
                     PRQVATRVDPRPKRPARRRKRPSEEPTADAEVLAEYRRKRDFELTPEPPPTPKPGRDE
                     LFVIQKHDATRLHYDLRLERNGVMPSWAVPKGLPITTDEKRLAVKTEDHPIDYASFEG
                     WIPEGEYGAGEVRIFDTGRYELLEWEDDKVSFRLHGRRQRGEYHLVKTRTDWLIFLSK
                     RSLDEQPPSPSEVIPMMAEGGHQPFDHPDWRFEPKLDGVRTLAYVRTDSTKLVSRRGR
                     DQTATYPELANLARWVNALQAVIDAEIVAPEEDGRPSFERLQQRMNLQSKSEIERARK
                     KIPVALFAFDLLWLDGRDLTHVPLEQRRRLLEEIVTESGPVNLTFYTDGKGSPLFEAA
                     REQGLEGVIAKRLGTPYLPGRRSKDWRKIKALNEQDCVILGMTPGTGSRSPRFGALLV
                     GAYVDGALTWIGQVGTGFTDASLDDLLKRFEPLLTEKPAVDDPEVRKVKGARWLKPEL
                     VCEVEYLEMTKVGKLRAPSFKGLRPDKAPQDCILERPAR"
     gene            complement(3822..4469)
                     /locus_tag="VG709_04780"
     CDS             complement(3822..4469)
                     /locus_tag="VG709_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWC32424.1"
                     /translation="MSAIERTLVKPRWRGRLHQVAFFVSLPLGGALGAMAEGSARVAS
                     VIYALTLSGMYAASAAFHRLEWGPRAWAWMRRLDHSMIFLLIAGTYTPYALLVLQPPW
                     STTVLAVVWGGALVGVTLRMLSRGLHWLQQTLYMVLGWAALFTLPLMISRLEPGAIAL
                     MFAGGLFYTIGAVMFSLRRPDPNPLVFGYHEVWHVMVTSGSLCHYVLVLWLVLRV"
     gene            4531..5745
                     /locus_tag="VG709_04785"
     CDS             4531..5745
                     /locus_tag="VG709_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32425.1"
                     /translation="MPRRVLIAAAVAGTLLMGVVPAEAVRPPLLPYRGLGAWIDIYDD
                     EAWDRPAATARTIAARGVRTLYLETCNFRCERDLFRPAAMARLLRASHVEGMKVVAWY
                     LPGFQNVRRDVRRARKAIEFETSDGHRFDGFALDIEAMEDSPVEERNRRVRVVSRRLR
                     ELVGAGYALGAITPHGEYPWDPFPYRMLGLYYDVFLPMNYFAVRDSGARAARRHTAFN
                     IRTIREETGIRNAPIHIIGGVADEMNRKEAVAFVRTAREHGVLGASAYDVFTSGPEDW
                     RAMRSVPANPRQSPALPVALGYARPLGNLPFADRTHPKEVFFRLGGRAAGATLRFDAY
                     GIQRDEVSVVVNWRRVGDVRATGGDWKRRRVAIPARLLRDRGPNTIGFIADGRFPNWR
                     VWGVREVRLVAT"
     gene            complement(5749..6255)
                     /locus_tag="VG709_04790"
     CDS             complement(5749..6255)
                     /locus_tag="VG709_04790"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HWC32426.1"
                     /translation="MDEEPDQPEPLDEEEREDIEADLDDLKAMRAVFEAQGMRGVVVA
                     CPDCGSDHYYDWDLLRESLEHMLETGEPRMHEPAFEPREEDYVLWDYGKGYVDALSDA
                     GLDDDTTIDVHRCAWCETPLEPHYGYCPKCGRPSAVGRLLKELVGRGMDEREARALLV
                     KAGFEPFS"
     gene            complement(6255..6974)
                     /locus_tag="VG709_04795"
     CDS             complement(6255..6974)
                     /locus_tag="VG709_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWC32427.1"
                     /translation="MGFARAQVVFAHPDDAEYLCGGTVATWSDGGTEVEYVCATDGSA
                     GWNGPDRTREEIAATRETELRDAAGILGVRSVRFLGFVAGALEPTLELRRAVTREVRR
                     FRPDVIVTIDPTRRWIGRSYINHPDHRAIGDAVLAVVACDAPTRPQFAELLDEGLEPW
                     KVAALWLATGDHEGDVVVDIADTIDRKIESLRVHASQMENMGGFDPERIRDWSRRCAK
                     DADMEYGEAFRTFSLADEERE"
     gene            7048..>7497
                     /locus_tag="VG709_04800"
     CDS             7048..>7497
                     /locus_tag="VG709_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWC32428.1"
                     /translation="MNVTASRRRPENRILVRSALPTLLALGAGWFAVQAALGGRASPL
                     VRSGVGAAVVVAFVAGVRAAWVAARELRIERRRLEGRRSEIDELLARERETVRRLHEI
                     SRMKSDFVSIASHELRTPLTSIRCANSERNTSFSATCGRRSVAAIPRI"
BASE COUNT         1153 a         2636 c         2573 g         1135 t
ORIGIN      
        1 ggtcgggacg gaaccgcggg gaaccggtcg agccagctgt acgacgcccg cgcccacgtg
       61 gcgggcgcat acgcgtacgg ctcacgcccc tcgatgactg gagcatccgc agctcccacg
      121 ctcaccggcg cctggcaggt ctctcgcaag ctcgccgcgc cgccgacgtg gtcgccgtgg
      181 ctgtgggaga tcacgatctg gcggacgtcg gtcgggcgac gtcccatccg ctcgaaggcc
      241 gcggcgatcc gccgctcgct catccacgtg ccggtgtcca ctagcgtcac gacgccgcgg
      301 tgctccacga ggaacgcgcg cccgacccac cgcgcgccga tcgggacgac ctcgggcacg
      361 ggctacgagg ccgcgcgctt gcgagcggga gccttgcgct tcttcccggt ctccttcgcg
      421 gcttccttgc gggtgtcctc gacgctgcgc ttcaaggctt ccatgaggtc cgagaccttc
      481 gtcggctccg gcggctcgac gatctcgacc tcctcgccct cgaccttccg ctggacgatg
      541 tcgaggagcg cctctcggta ctcgtcggtg tagcggtcgg gcttccagtc ctcggtgagg
      601 ttgtcgatga ggctctgcgc catctggatc tcttggtcgc ggactttcac gtccttgtcg
      661 agctcgggga actccgcctc gcggatctcg tccggccagt acatcgtctc gagcacgagg
      721 acgccgtcgc gcagccggat cgtcgcgaga tgctccttgt cacggaacga gaccttggcg
      781 acgccgacct tgctgccgcg cgcgagcccc tcggatagca ggcggtacgc cttgacgccg
      841 gtctcctccg ggccgaggta gtatgtcttc ttgaagtaga tcgggtcgat ctcctcgagg
      901 tcgacgaact gctggatgtc gatcgttctc gtcgactcga ccgggaccgc atcgagatcc
      961 tcgtcgtcga ggatcacgta gcggtccttc tcgtactcgt agccccggac gatctcgtcg
     1021 tacgggacct ccttgtcctc gaccgtgcac cagcgcttgt accggatccg gccgtggtcc
     1081 ttctcgtgca actggttgaa gtggaggctc ttctcctcgg tcgccgtgta gagccgcacg
     1141 gggatcgaca cgagcccgaa cgagatggcc cccctccaga gcgtcctcat ggggatccct
     1201 ccagggttga gatggctcga ttgtatccct gaaatcgcag gtcgaacgcg ttttttgcgt
     1261 tcgactgcgg ataccatcgt cgcgtgccga ggaagaagcg aaccggcccg gcgttcacgg
     1321 tcgagacccc ggagccgctc ccggcgagcc gggagggaga ggcctggtgg ctcgagcgcg
     1381 acgggcgccg cgttcgcctc tcgaacctcg acaaggtctt ctggcccgag gagggctaca
     1441 cgaagggcga tctcctcgcg tactactaca acgtcgcgga gctcattctc ccgtacgtcc
     1501 gcgaacggcc gctcacgatg aggcggatgc ccgacgggat ggccggccac gagttctacg
     1561 agaagcgctc gccgccgaac acgccggagt ggatgtcgaa gtgcgtggtc ccggccgacg
     1621 atgagggcga gcgcgacatc gactacctcc tcgccgagga cgtgccgagc ttgctcttcg
     1681 tcgtgaacat gggtgcgatc gagttccacc cgctccactc gagatgcggg tcaatcgacc
     1741 ggccggacta ccttttcttc gacctcgacc cgttcccgcc cgcgacgttc gacgacgtgc
     1801 tcgcggtcgc gcggcacgtg cgtgcggcgc tcgacgcgct cggcctcccg tcgtacccca
     1861 agacgtccgg cgcaaccggc atgcaggtct acgtggggct cgatccgcag ccgacctacg
     1921 acgacgtccg cgggttcgtg cgcgcgctgt gccgccacat caacgccgcc gaccccgacc
     1981 gcacgacgat ggagtgggac atctcgaagc gcgccgggaa ggtgttcctc gactggaaca
     2041 tgaaccggct cggcgcgaac atcgcggccg cgtactcggt gcgcccggag cccggcgcga
     2101 ccgtctcgac cccgctctcg tgggacgagg tcgaagccgg cgtgacgccg cgggactgca
     2161 cgatcgcgaa cgcgcacgag cggttcgcgg atgtcggcga cctcttccgg gggatgctcg
     2221 aggaacccat cgacatccgc ccggcgatgg attcgatggg cgtgaagccc gacggggaga
     2281 cgtctccgcg ccaggtcgcg acgcgcgtcg accctcgtcc caagaggccg gctcggcggc
     2341 ggaagcggcc gtccgaggag ccgacggccg acgcggaggt gctcgcggag taccggcgca
     2401 agcgcgactt cgagctgacg cccgagcccc cgccgacgcc gaagcccgga cgcgacgaac
     2461 tgttcgtgat ccagaagcac gacgcgacgc gcctccacta cgacctccgg ctggagcgga
     2521 acggcgtgat gccgtcgtgg gcggtgccga aggggctccc gatcacgacc gacgagaagc
     2581 ggctggcggt caagaccgag gaccatccga tcgactacgc gagcttcgag ggatggatcc
     2641 ccgagggcga gtacggcgcc ggcgaggtgc ggatcttcga caccggcagg tacgagctcc
     2701 tcgagtggga ggacgacaag gtctcgttcc ggctccatgg gcggaggcag cgcggcgagt
     2761 accacctcgt caagacgcgg accgactggt tgatcttcct ctcgaagcgc tcgctcgacg
     2821 aacagccgcc gtcgccctcc gaggtgatcc cgatgatggc ggaggggggt caccagccgt
     2881 tcgaccaccc cgactggcgg ttcgagccga agctcgacgg ggtccgcacg ctcgcgtacg
     2941 tgcggaccga ctcgacgaag ctcgtgtcgc gccgcggccg cgaccagacc gcgacctatc
     3001 ccgagctcgc gaacctcgct cgctgggtga acgcactcca ggcggtgatc gacgccgaga
     3061 tcgtcgctcc cgaggaggac ggccggccgt cgttcgagcg cctccagcaa cggatgaacc
     3121 tgcagtcgaa gtcggagatc gagcgcgcgc ggaagaagat ccccgtcgcg ctgttcgcgt
     3181 tcgatctcct ctggctcgac ggccgcgacc tgacgcacgt gccgctcgag cagcggcgcc
     3241 ggctcctcga ggagatcgtg acggagtcgg gccccgtgaa cctcacgttc tataccgacg
     3301 gcaagggttc gccgctgttc gaggcggccc gcgagcaggg cctcgagggc gtcatcgcga
     3361 agcggctcgg gacgccgtac ctgccgggga gacggtcgaa ggactggcgc aagatcaagg
     3421 cgctcaacga gcaggactgc gtgatcctcg ggatgacgcc gggcacgggc tcacggtcgc
     3481 cgcgcttcgg cgcgctgcta gtcggcgcct acgtcgacgg cgcgctcaca tggatcgggc
     3541 aggtggggac cggcttcacc gacgcgtcgc tcgacgacct cctgaagcgg ttcgagccgc
     3601 tcctcaccga gaagcccgcg gtcgacgacc ccgaggtgcg caaggtcaag ggcgccaggt
     3661 ggctgaagcc cgagctcgtc tgcgaggtcg agtacctcga gatgacgaag gtcgggaagc
     3721 tccgcgcgcc gtcgttcaag ggcctccggc ccgacaaggc gccgcaggac tgcatcctcg
     3781 aacgaccggc gcgatgacca tgacgcccga cgctccgcgc gtcagacgcg cagcacgagc
     3841 cacaagacga gcacgtagtg gcacaggctt ccgctcgtca ccatgacgtg ccagacctcg
     3901 tggtacccga agaccagcgg gttcgggtcg ggccggcgta gggagaacat gacggcgccg
     3961 atcgtgtaga agagcccgcc cgcgaacatg agggcgatcg cgccgggctc cagccgcgag
     4021 atcatgagag ggagcgtaaa aagcgcggcc cacccgagca ccatgtacag cgtctgctgc
     4081 agccagtgga gcccgcgcga cagcatccgg agcgtgacac cgacgagcgc gccgccccac
     4141 acgacggcga ggacggtcgt cgaccaaggc ggctggagga cgagcagcgc gtacggcgtg
     4201 tacgtgccgg cgatgagcag gaagatcatc gagtggtcga gccggcgcat ccatgcccag
     4261 gcgcgcggcc cccattcgag ccggtggaag gccgcgctcg ccgcgtacat ccccgagaga
     4321 gtcagcgcgt agatcacgct cgcgacccgt gcggaaccct cggccatcgc gccgagcgca
     4381 ccgccgagcg gcaacgagac gaagaacgcc acctgatgaa ggcggccgcg ccaccgcggc
     4441 ttgacgagcg tgcgctcgat cgcggacatg gcagcgaagg atagcgcacg gcctcatcgg
     4501 aagagccgga cccgccgata gactggcccg atgccacgtc gcgtcttgat cgccgccgcg
     4561 gtcgccggaa ccctcctcat gggcgtcgtt cccgcggagg cggtgcggcc cccgttgctc
     4621 ccctaccggg gtctcggcgc ctggatcgac atctacgacg acgaggcgtg ggaccggccg
     4681 gcggcgacgg ctcggacgat cgcggcgcgc ggcgtgcgca cgctctacct cgagacctgc
     4741 aacttccgct gcgagcgcga ccttttccgc ccggccgcga tggcgcgact cctgcgagcg
     4801 tctcacgtgg aagggatgaa ggtcgtcgcg tggtatctcc ccgggttcca gaacgtccgt
     4861 cgagacgttc ggcgcgccag aaaggcgatc gagttcgaga cctccgacgg acaccggttc
     4921 gacgggttcg cgctcgacat cgaggcgatg gaggactccc cggtcgagga gcggaacaga
     4981 agggtccgcg tggtctcgcg ccgcctgcgc gagctcgtcg gagccggcta cgcgctcggg
     5041 gcgatcacgc ctcacggcga gtacccgtgg gacccgttcc cctaccggat gctcgggctc
     5101 tactacgacg tgttccttcc gatgaactac ttcgcggtca gggactcggg cgcgcgggcg
     5161 gcccggcgcc acaccgcctt caacatccgg acgatccgcg aggagaccgg gatcaggaac
     5221 gccccgatcc acatcatcgg cggtgtcgcc gacgagatga accgcaagga ggccgtcgcg
     5281 ttcgtccgga cggctcggga gcacggcgtt ctgggcgcca gcgcctacga cgtgttcacc
     5341 tcgggcccgg aggactggcg cgcgatgcgg agcgtccccg cgaacccccg gcagtcgccc
     5401 gcgcttccgg tcgcgctcgg ctacgcgcgg ccgctcggga acctgccgtt cgccgaccgg
     5461 acgcatccca aggaggtgtt cttccggctc ggggggcgtg ccgccggcgc gacgctgcgc
     5521 ttcgacgcct acggcatcca gcgcgacgag gtctccgtcg tcgtgaactg gagacgcgtc
     5581 ggcgacgtgc gtgcgacggg aggcgattgg aagcggcgcc gcgtcgcgat cccggcacgg
     5641 ctgttacggg accgcgggcc gaacacgatc gggttcatcg cggacggacg gttcccgaac
     5701 tggcgggtgt ggggcgtgcg ggaggtgcgg ctcgtggcga cgtagttgct acgagaaagg
     5761 ctcgaacccc gccttcacga gaagcgcccg cgcctctcgc tcgtccatcc cgcgtccgac
     5821 aagctccttg agcaggcgcc cgacggcact cggccgcccg cacttcgggc agtagccgta
     5881 gtgcggctct agcggcgtct cgcaccacgc gcagcgatgg acgtcgatgg tggtgtcgtc
     5941 gtcgagcccg gcatccgata gcgcatcgac gtagcccttg ccgtagtccc acagaacgta
     6001 gtcctcctcg cgcggctcga acgccggctc gtgcatccgg ggctcaccgg tctcgagcat
     6061 gtgctcgagg ctctcgcgga gcaggtccca gtcgtagtag tggtcggagc cgcagtcggg
     6121 acaggcgacg acgaccccgc gcatgccttg cgcctcgaag acggcgcgca tcgccttgag
     6181 gtcgtcgagg tcagcctcga tgtcctctcg ctcttcctca tcgagcggct ctggttggtc
     6241 gggctcttcg tccatcactc ccgctcctcg tccgccagcg agaaggtcct gaacgcctct
     6301 ccgtattcca tgtcggcgtc cttcgcgcag cgacgcgacc agtcgcggat ccgttccggg
     6361 tcgaagccgc ccatgttctc catctggctc gcgtgcacgc ggagcgactc gatcttccga
     6421 tcgatcgtgt ctgcgatgtc cacgacgacg tcgccctcgt ggtcgccggt cgccagccag
     6481 agcgccgcca ccttccacgg ctcgagcccc tcgtcgagca gttcggcgaa ctgcgggcgc
     6541 gtcggcgcgt cgcacgccac gacggcgagc acggcgtcgc cgatcgcccg gtggtcggga
     6601 tggttgatgt acgagcgccc gatccaccgc ctcgtcgggt cgatcgtcac gatcacgtcg
     6661 ggccggaacc gccggacctc gcgcgtgacg gcgcgccgga gctccagcgt cggctcgagc
     6721 gccccggcga cgaacccgag gaagcggacc gagcgaacgc cgaggatccc ggccgcgtcc
     6781 ctgagctccg tctcgcgcgt cgcggcgatc tcctcgcgcg tccggtcggg gccgttccat
     6841 cccgcggagc cgtcggtcgc gcacacgtac tcgacttccg ttccgccgtc gctccacgtc
     6901 gcgaccgtgc cgccgcagag gtactcggcg tcgtcggggt gggcgaagac gacctgcgcc
     6961 cgcgcgaatc ccatctccgg ccaagcgtac cggatggcgc ccgttcggcc tccctcaagt
     7021 cggggcgcgg catcgccgag aaccagggtg aacgtgaccg cgagcaggcg ccgcccggag
     7081 aaccggatcc tcgttcgctc ggcgctgccc accctgctcg cactcggcgc cgggtggttc
     7141 gccgtgcagg cggccctcgg cggccgcgcg tcgccgctcg tgcggagcgg cgtcggcgcg
     7201 gcggtcgtcg tcgcgttcgt ggccggcgtc cgggcggcgt gggtcgccgc ccgcgagctg
     7261 cgaatcgaac gccgccgcct cgaggggcgc aggagcgaga tcgacgagct gctggcccgc
     7321 gagcgcgaga ccgtaagacg cctccacgag atcagccgga tgaagagcga cttcgtgtcg
     7381 atcgcctcgc acgagctccg caccccgctg acctcgatcc gctgcgcgaa ctccgagcgc
     7441 aacacctcgt tctccgcgac ctgcggtcgt cgcagcgtcg ctgccatccc gaggatc
//
LOCUS       DASZOB010000292         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_33270, whole genome shotgun sequence.
ACCESSION   DASZOB010000292 DASZOB010000000
VERSION     DASZOB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33270"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VG709_04805"
     CDS             <1..215
                     /locus_tag="VG709_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32429.1"
                     /translation="LPLESGWAHPDPAMLEFLQSSGLPTPERQGDARVGAAWNPRPAF
                     VGIVVLGALFLGAAAIDRRRRVAPTP"
     gene            complement(228..497)
                     /locus_tag="VG709_04810"
     CDS             complement(228..497)
                     /locus_tag="VG709_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32430.1"
                     /translation="MPRRHRSARERGEPEPLTPPRGAVPAWARIEDVDVYLSTKGGSR
                     CPGCNQMMRDGASHLVVIERGDLAGRRHWHIPCWRTELRRRGRAR"
     gene            complement(505..1131)
                     /locus_tag="VG709_04815"
     CDS             complement(505..1131)
                     /locus_tag="VG709_04815"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWC32431.1"
                     /translation="MKVETPAGTVSATRSGPESPVVVVAHGAGGSKDDPLLEGFAAAL
                     GERGVGCLRFNFPYREHGRKAPDPEPVLRDAWAAAFETAGGAEVWVGGKSLGGRIASM
                     CVADGMPARGVVFVGYPLHPPGRPERIRDEHLYRVPVPMLFLQGTADPFARWDLLEGV
                     LSRIGDRATLHAVEGGGHSFRVRGTRKDDRGTGRRLGEVAAEFVLARR"
     gene            complement(1128..>2384)
                     /locus_tag="VG709_04820"
     CDS             complement(1128..>2384)
                     /locus_tag="VG709_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWC32432.1"
                     /translation="DVHLLRTVGEVHDLVGSAGGDAVAARAAAAVRDPDALRLGALLH
                     DIGKTGEGGHIPRGAAIAAGVLDRMRVSGALRDDVLFLVREHLLLSDTATRRDLSDEN
                     LVLDVAARVGSHERLAMLYLLTAADARATGPHAWTPWRQALIRELVSKVERTLEREPA
                     DRTATLTSTMDAVRALLRTEDQRAVDEYLARIPRPYLLAVPPDVAARHFHLVIPPPAR
                     GDVRTDTAAGSREGTYELTVVAADRPGLLAKVAGALSLSGLSILSAQAFTTEDGVAID
                     LFVVEPAFHGDVDEERWRRFRSDLRKALAGRISLEYRVREKRRHYPAPTADVPTEVTV
                     ANDASDFYTVVEVSTADRIGLLFDLARTFQELELDVHLAKVATYGVRVVDAFYVRDLF
                     GQKVEDERHVRESERASVARLEPERP"
BASE COUNT          339 a          872 c          867 g          306 t
ORIGIN      
        1 ccctgccgct cgaaagcggc tgggcgcatc cggaccccgc catgctcgag ttcctccagt
       61 cgagcggtct gccgacgccc gagcggcagg gcgacgcgcg cgtcggcgcc gcatggaacc
      121 cccgaccggc cttcgtgggg atcgtggttc tcggggcgct cttcctcggt gcggccgcga
      181 tcgacaggcg gaggcgcgtg gctcccacac cttgaggagg cgatcgatca gcgagcgcgc
      241 ccgcgcctgc gcagctcggt gcgccagcac gggatgtgcc agtgccgccg tcccgcgagg
      301 tcgccccgct cgatgacgac gagatgcgag gcgccgtctc gcatcatctg gttgcatccg
      361 ggacagcggc ttccgccctt cgtcgagaga tagacgtcga catcctcgat ccgggcccac
      421 gcgggcaccg cgccgcgcgg cggtgtgagg ggctcgggct cgccgcgctc acgggcggat
      481 cgatgacgac ggggcatgca tcgattatcg ccgcgcgagg acgaactccg ccgcgacctc
      541 gccgagacga cgcccggttc cgcggtcgtc cttccgcgtg ccgcgcaccc tgaaggagtg
      601 gcccccgccc tcgaccgcat ggagcgtcgc gcggtcgccg atccgagaca ggaccccctc
      661 gagcagatcc catctcgcga aggggtcggc ggtcccctgc aggaacagca tcgggacggg
      721 gacccggtac aggtgctcgt cgcggatccg ctccggccgg cccggaggat ggagcgggta
      781 gccgacgaag acgacgccgc gcgccggcat cccgtcggcc acgcacatcg aggcgatccg
      841 accaccgagc gacttcccgc cgacccagac ctccgcgccg cccgcggtct cgaaggccgc
      901 ggcccaggcg tcacgaagga ccggctcagg atccggcgcc tttcgaccgt gctcgcgata
      961 cgggaagttg aaccgcaggc acccgacgcc tcgctccccg agcgcggcgg cgaacccctc
     1021 gagcagcggg tcatccttgc tgccgccggc gccgtgcgcg acgacgacga ccggagactc
     1081 cgggcccgat cgcgtcgcgg agacggtgcc cgccggcgtc tcgaccttca cgggcgctcc
     1141 ggctccagac gcgcgacgct cgcgcgttcg ctctctcgca cgtgccgctc gtcctcgacc
     1201 ttctggccga acagatcgcg aacgtagaac gcgtccacga cgcgcacgcc gtacgtcgcg
     1261 accttcgcga ggtgcacgtc gagctcgagc tcctggaacg tccgcgcgag gtcgaacagc
     1321 agcccgatgc gatccgccgt cgagacctcc acgaccgtgt agaagtcgga ggcgtcgttc
     1381 gccaccgtga cttcggtcgg cacgtcggcc gtcggcgcgg gatagtgccg ccgcttctcg
     1441 cgcacgcggt actcgagcga gatccggccg gcgagggcct tgcggaggtc ggagcggaac
     1501 cggcgccagc gctcctcgtc gacatcgccg tggaaggccg gctcgacgac gaagaggtcg
     1561 atggcgacgc cgtcctcggt ggtgaacgcc tgcgccgaga ggatactgag gccggacagc
     1621 gagagcgcgc cggcgacctt ggcgaggagc ccagggcggt cggccgcgac gaccgtcagc
     1681 tcgtacgtcc cctcgcgcga gccggcagcc gtgtcggtcc gcacgtcgcc acgggcgggc
     1741 ggcgggatca cgaggtggaa gtgcctcgcg gcgacatcgg gcggcacggc gaggagatac
     1801 ggacgcggga tccgcgcgag gtactcgtcg accgcgcgct ggtcctcggt ccgcagcaag
     1861 gcccgcaccg cgtccatcgt cgacgtgagc gtcgccgtac gatcggccgg ctcgcgctcg
     1921 agcgttcgct cgaccttcga caccagctcg cggatcaggg cctggcgcca cggcgtccac
     1981 gcatgcggcc ctgtcgcgcg cgcgtccgcg gccgtgagga ggtacagcat cgcgaggcgc
     2041 tcgtgggacc cgacacgcgc cgcaacgtca aggacgaggt tctcgtcgga caggtcgcgc
     2101 cgcgtggcgg tgtcggagag caggaggtgc tcgcggacga ggaacagcac gtcgtcgcgg
     2161 agcgccccgg acacgcgcat gcggtcgagg acgccggcgg cgatcgcggc gccgcggggg
     2221 atgtggccgc cttcgccggt cttcccgatg tcgtggagga gcgccccgag ccgcagcgcg
     2281 tcgggatcgc gaaccgccgc cgccgcgcgg gcggcgaccg cgtcgcctcc tgccgatccg
     2341 acgaggtcgt ggacctcgcc gacggtgcgg agcaggtgca cgtc
//
LOCUS       DASZOB010000293         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_56697, whole genome shotgun sequence.
ACCESSION   DASZOB010000293 DASZOB010000000
VERSION     DASZOB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56697"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /gene="groL"
                     /locus_tag="VG709_04825"
     CDS             complement(<1..1586)
                     /gene="groL"
                     /locus_tag="VG709_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851179.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWC32433.1"
                     /translation="MPAKQLKFDEEARRALEAGIDRLADAVSVTLGPKGRNVVLDKKW
                     GAPTITNDGVTIAKAVELDDPWENLGAQLAKEVATKTNDVTGDGTTTATVLARAMVHH
                     GMRNVAAGANPMGLKRGIERAVEAVTKVIEQQSREVEGKDEIAHVAAISAADREIGEV
                     IAEALDKVGKDGVVTVEEGQTFGMELEFTEGMQFDKGYISPYFVTDQDRQEAVLEDPY
                     ILLADKKISAVSDLLPILEKVAQSGRPLLIIGEDVEGEALATLVVNKIRGTFRSAAVK
                     APGFGDRRKAMLQDMATLTGAQVASEDIGVKLEGVTLDMLGRARRVVITKDDTTIVEG
                     AGDAEEIKGRIGQIRAEIENTDSDWDREKLQERLAKLSGGVALIKVGAATEVELKERK
                     HRIEDALSATRAAVEEGIVPGGGVALLRAEQELRNLGLEGDEATGASIVREALAEPAR
                     LIASNAGYEGAVVVERLRGEDGARGFDAERGAWVDLFDAGIVDPAKVTRSALQNAASI
                     AGLVLTTESAVVEKPEEEDE"
     gene            complement(1630..1932)
                     /gene="groES"
                     /locus_tag="VG709_04830"
     CDS             complement(1630..1932)
                     /gene="groES"
                     /locus_tag="VG709_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWC32434.1"
                     /translation="MAKGKFGIEPLEDRVVVKPADEEQTTPTGIVIPDTAKEKPQEGE
                     VVAVGPGRFEDGQRVPVDVKAGDRVLYSKYGGTEVKVEGDEYLVLSARDLLAIVRR"
BASE COUNT          257 a          802 c          683 g          359 t
ORIGIN      
        1 tcctcgtcct cctcctcggg cttctcgacg accgcgctct cggtggtgag gacgagcccg
       61 gcgatcgacg ccgcgttctg gagagcggac cgcgtcacct tcgccgggtc gacgatcccc
      121 gcgtcgaaga ggtccaccca cgctcctcgc tcggcgtcga acccgcgagc gccgtcctcc
      181 ccgcggagcc gctcgacgac gaccgcgccc tcgtagcccg cattgctcgc gatgagccgg
      241 gccggctcgg cgagcgcctc gcggacgatg gaggcgccgg tcgcctcgtc gccctcgagc
      301 ccgaggttgc ggagctcctg ctcggcgcgg agcagcgcga cgccgccgcc agggacgatg
      361 ccctcctcga cggccgcgcg cgtcgccgag agcgcgtcct cgatgcggtg cttgcgctcc
      421 ttgagctcga cctcggtcgc ggcgccgacc ttgatgagcg cgacgccgcc cgacagcttc
      481 gcgagccgct cctgcagctt ctcgcggtcc cagtccgagt cggtgttctc gatctcggcg
      541 cggatctggc cgatgcgccc cttgatctcc tcggcgtcgc cggcgccctc gacgatcgtc
      601 gtgtcgtcct tcgtgatcac gacgcgccgc gcgcgcccga gcatgtcgag cgtcacgccc
      661 tcgagcttga cgccgatgtc ctcggacgcc acctgagcgc cggtcagcgt cgccatgtcc
      721 tggagcatcg ccttgcggcg gtcgccgaac ccgggcgcct tgacggccgc cgagcggaac
      781 gtgccgcgga tcttgttgac gacgagcgtg gcgagcgcct cgccctccac gtcctcgccg
      841 atgatgagga gcgggcggcc cgactgcgcc accttctcga ggatcgggag caggtccgac
      901 accgccgaga tcttcttgtc ggcgagcagg atgtagggat cctcgagcac cgcctcctgg
      961 cggtcctggt cggtcacgaa gtacggcgag atgtagccct tgtcgaactg catcccctcc
     1021 gtgaactcga gctccatccc gaaggtctgg ccctcctcga ccgtgacgac gccgtccttg
     1081 ccgaccttgt ccagcgcctc ggcgatgacc tcgccgatct cgcggtcggc cgcggagatc
     1141 gcggcgacgt gcgcgatctc gtccttgccc tcgacctcgc gggactgctg ctcgatgacc
     1201 ttcgtgacgg cctcgaccgc gcgctcgatc ccgcgcttga gacccatcgg gttggcgccg
     1261 gccgccacgt tcctcatccc gtgatggacc atcgcgcggg cgaggaccgt cgcggtcgtc
     1321 gtgccgtcgc cggtcacgtc gttggtcttg gtcgcgacct ccttggcgag ctgcgccccg
     1381 aggttctccc acgggtcgtc gagctcgacc gccttggcga tcgtgacgcc gtcgttcgtg
     1441 atcgtcggcg ccccccactt cttgtcgagg acgacgttgc ggcccttcgg gccgagcgtc
     1501 acggagaccg cgtcggcgag ccggtcgatg ccggcctcga gcgcgcgacg cgcctcctcg
     1561 tcgaacttca gctgcttcgc tggcatacgg aatcctccct gccgccggag cggacggtct
     1621 cgtgccggac taccggcgga cgatcgcgag caggtcgcgc gccgagagga cgaggtactc
     1681 gtccccctcg accttgacct cggtgccccc gtacttggaa tagaggacgc ggtcgccggc
     1741 cttcacgtcg accgggaccc gttgcccgtc ctcgaagcgg ccgggcccca cggccacgac
     1801 ctcgccctcc tgcggcttct ccttcgcggt atcggggatc acgatgccgg tcggcgtcgt
     1861 ctgctcctcg tcggcaggct tgacgacgac gcggtcctcg agcggctcga tcccgaactt
     1921 gcctttcgcc atgtcgcact cctcctcgtt tagcactcgg ctacctagag tgctaacagt
     1981 ctagcgaagc gggccccacc cgagcaagac ggtcgacgcg ccgtcggcgc cgcggccgcg
     2041 aaccgagggc ccgccacgcg cctgcgccgg ctcccctatc cgcccttcag gaacgcgacg
     2101 g
//
LOCUS       DASZOB010000294         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_43482, whole genome shotgun sequence.
ACCESSION   DASZOB010000294 DASZOB010000000
VERSION     DASZOB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43482"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1201)
                     /locus_tag="VG709_04835"
     CDS             complement(5..1201)
                     /locus_tag="VG709_04835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWC32435.1"
                     /translation="MADTDERQESAYTRMGEQWLLRGGGVDFVTTQGRDLSTEEMVLN
                     IGPQHPSTHGVLRLVIELDGEKIVRAEPVIGYMHRGAEKLAEHRDMTQTLVLMNRHDW
                     LSAFNNELGWCIAVERLAGIEVPERAQWIRTMMAEWNRILNHLMFTGSFPLELGAMTP
                     MFYAFREREMIQGLMESATGARMHHSFARVGGLKDDLPRGFLKHSAEVLTWVRKKLRD
                     FDNLIMGNEIILARTKGVGMLPADVAISYGVSGPVLQASGVPMDPRKDEPYEKYGDVE
                     FDVPVGTNGDSYDRLWVLVQRMWESCKIIEQCMDRLPPGPFITSKLPRNIPEGEVYVR
                     NENPLGLMGYYLVGNNKKYPYRLKMRTASFSNVSVLPWMLQGKLVPDLIAILGSVCFV
                     VGDVDK"
     gene            1287..1781
                     /locus_tag="VG709_04840"
     CDS             1287..1781
                     /locus_tag="VG709_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32436.1"
                     /translation="MARKTLVGAALPLAILLAATPTVATDHRPPTPTLVVQGRAQAAR
                     VYTTCWATGSGGFCADGTPSFGRAIRAQPTRGARIRIPHPAAIEDVDLHGWARNPDPD
                     WEFRRAREFRVRRRALSSDGERTGWLLLFRLPRREGHLYLHLSLEWRHRGDLFGTFHA
                     LLRK"
     gene            complement(1845..2588)
                     /locus_tag="VG709_04845"
     CDS             complement(1845..2588)
                     /locus_tag="VG709_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWC32437.1"
                     /translation="MALRARVIRSQCIGAGNCIYIAPTAFSWQKGDQHGKADVLDPSS
                     VEEELLREAALACPTQAIEIDETDEILPLQVGLVRQVASTRVERTFMFTDIARSTVLV
                     EALGDEAWESLIRWHDETLRALFAEHGGEVIHHTGDGFFVAFPAQSAAIDCAVAIQRR
                     LAEQRKTQGFAPEVRIGLHASEATRVDGDFRGKGVHEAARIAGLAAGGEITASRHTVE
                     GSSVRTSEPRTVELKGIAEPVEVVEISWR"
     gene            complement(2588..>5571)
                     /locus_tag="VG709_04850"
     CDS             complement(2588..>5571)
                     /locus_tag="VG709_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWC32438.1"
                     /translation="LTAISDEELRGPLLVARSLFGSSAARALAAAAGQEEQLDRLLDA
                     LSGRPDASAMALPPDQQLIRAYDLAAVTLGGLAAHAPVALLLDDVQWADQDSIRLLRY
                     IVRTEPTSRVLVVLALRPTAEAPREVSTLLADLERLRVAWRLRLERFGSVESGELLRH
                     VLGGNLAPSTLEAIHSQAEGVPFMLEELAQAYRETGLLQRIDAEWQLAPRAERLLPRS
                     VEMLIRRRVDRLDDATRTSLAEAAVISRTFGLADLLSVKQRLGDPETTVAELATSLAP
                     AAAAGLLSELPEGWSADYRFTHEQIRSFAAASLPRARRREIHAAIVDLLLSGGDPPPE
                     AMAMVAHHAVAAGLGEQCARYSVQAARAALEGHAPDEVLRLVDESLATSTNPGNRVEL
                     LRLRDDALGALRRDRERLETLPQLAALADAIGDVALGIEVTLRRAAALRLSGDEDRAA
                     ELSEQARERARAAGADEAELRACLELGQAILRSPLGEAAGTAAGDAELERAEEAYQRA
                     AELAAELGHRASEAAAWRELGSIALERARKHFYAFMASPERPSLRQAPPREFLFTVPG
                     LPERFQPAIERFGKALELYEAVGDQRGMMTTLIAMAYVRTGPDIRFGSARRIEDLRRI
                     VSRRHTLVRESERERHDVQMLYGVHLYARAFGFPDLALERGQDAYRGALRIGDRDLEF
                     LAAGGTALAYLAMGEVSEATEWLERATTAAAAAPSSLRARHLEMWRGMAAAASGDVAG
                     MRERLERALQIAVERGTAASRCEVLARLALEAAQLAAGGADEELLREADAWAADARRL
                     AASLRGDHPWTAQAEAAMALLHATRGRNEEARGSARAALQALEGLGFRESDPPYPLHL
                     DVVLAALRALDGSPDADEAKTLARTILQMVGERTTDENVRVRWFRAPAQRALAELTGP
                     VEVASPTTDADDGVLPGGLSDREAEMLRMITDGMTNKEIAGALGIGEDEVVRDLATIY
                     AKLGVGSRAGATVYALLEGVA"
BASE COUNT          798 a         2032 c         1933 g          808 t
ORIGIN      
        1 gtcgctactt gtcgacgtcg ccgacgacga agcacacgga gcccaggatc gcgatgaggt
       61 cggggacaag cttcccctgc agcatccacg gcagcaccga gacgttcgag aacgaggccg
      121 tccgcatctt caggcggtac gggtacttct tgttgttccc gacgaggtag tagcccatga
      181 gccccagcgg gttctcgttg cggacgtaca cctcgccctc ggggatgttc cgcgggagct
      241 tcgacgtgat gaacgggccg ggcgggaggc ggtccatgca ctgctcgatg atcttgcacg
      301 actcccacat ccgctggacg aggacccaga gccggtcgta cgagtcgccg ttcgtgccga
      361 cgggcacgtc gaactcgacg tcgccgtact tctcgtacgg ctcgtccttg cgcgggtcca
      421 tcggcacgcc cgacgcctgg agcaccggtc cggagacgcc gtacgagatc gcgacgtcgg
      481 ccggaagcat gccgacgccc ttcgtccggg cgaggatgat ctcgttcccc atgatcaggt
      541 tgtcgaagtc ccggagcttc ttccggaccc acgtcaggac ctccgccgaa tgcttgagga
      601 agccccgcgg gaggtcgtcc ttcagcccgc cgacgcgcgc gaacgagtgg tgcatccggg
      661 ccccggtcgc ggactccatg aggccctgga tcatctcgcg ctcgcggaac gcgtagaaca
      721 tcggcgtcat cgcgccgagc tcgagcggaa acgaacccgt gaacatcagg tggttcagga
      781 tccggttcca ctccgccatc atcgtgcgga tccactgcgc cctctccggg acctcgatcc
      841 cggcgagccg ctcgaccgcg atgcaccacc cgagctcgtt gttgaacgcc gagagccaat
      901 cgtggcggtt catgagcacg agcgtctggg tcatgtcgcg gtgctcggcg agcttctcgg
      961 cgccacggtg catgtagccg atgaccggct cggcacggac gatcttctcg ccgtcgagct
     1021 cgatgacgag gcgcagcacg ccgtgcgtcg acggatgctg tggcccgatg ttgaggacca
     1081 tctcctccgt ggagaggtcg cgtccctgcg tcgtgacgaa gtcgacgccg ccgccgcgga
     1141 ggagccactg ctcccccatg cgcgtgtacg cggactcctg ccgttcgtcg gtgtcggcca
     1201 tcggaaggct aagcgtatcg gaacgccgaa ccgatccgcc acgagggagt tcccaagtca
     1261 ggcgacgagc cccatactgg ggcccgatgg cgcggaagac cctcgtcggc gcggcgctgc
     1321 cgctcgcgat cctcttggcg gcgaccccca cggtcgcgac cgaccatcgg ccccccaccc
     1381 cgacgctcgt cgtccagggt cgggcgcaag ccgcacgcgt ctacaccacg tgctgggcca
     1441 cgggctccgg cggcttctgc gcggacggca cgccgagctt cgggcgggcg atccgcgcac
     1501 agccgacgcg cggcgcgagg atcaggatcc ctcatccggc ggcgatcgag gacgtcgacc
     1561 tccacggctg ggcacgaaac ccggaccccg actgggaatt ccggagggca cgcgagttcc
     1621 gcgtgcgccg ccgcgccctt tcctccgacg gcgaacgaac cgggtggctg ctcctgttcc
     1681 gcctgccgag gcgcgagggc cacctgtacc tgcatctctc gctcgagtgg cgccaccggg
     1741 gcgacctgtt cggcacgttc cacgccctcc tccggaagta gccgcgcccg tcgcgtcggg
     1801 gcgatagaac cgcgaggtcg ccgtagtggt tgaccgcggt tgaactaccg ccaggagatc
     1861 tcgacgacct cgacgggctc ggcgatcccc ttgagctcga ccgtccgcgg ctcggaagtg
     1921 cgaacggagc tcccctcgac ggtgtgacgg ctcgccgtga tctcgccacc cgccgcgaga
     1981 ccggcgatcc gggcggcctc gtggacgccc ttcccgcgga agtcgccgtc gacgcgcgtc
     2041 gcctcggacg cgtgcaggcc gatccgcact tccggcgcga atccctgggt cttccgctgc
     2101 tcggccagtc gacgctggat cgcgaccgcg caatcgatcg ccgccgactg cgcggggaac
     2161 gcgacgaaga aaccgtcgcc ggtgtggtgg atcacctcgc cgccgtgctc cgcgaacagc
     2221 gcgcggagcg tctcgtcatg ccagcggatc aggctctccc acgcctcgtc gccgagcgcc
     2281 tcgacgagga ccgtcgatcg agcgatgtcg gtgaacatga aggtgcgctc gacgcgagtc
     2341 gacgcgacct gccgcacgag cccgacctgc aacgggagga tctcgtccgt ctcgtcgatc
     2401 tcgatcgcct gggtcgggca cgcgagcgcc gcctcccgca gcagctcctc ctcgacgctc
     2461 gacgggtcga gcacgtccgc cttcccgtgc tggtcgccct tctgccacga gaacgccgtc
     2521 ggcgcgatgt agatgcagtt gccggccccg atgcactgag agcggatgac ccgagctctg
     2581 agcgccatca ggcgacgccc tcgaggagcg cgtacaccgt ggcgcccgca cgcgatccga
     2641 ccccgagctt cgcgtagatc gtggcgaggt cgcggaccac ctcgtcctcc ccgatgccga
     2701 gcgcgcccgc gatctccttg ttcgtcatcc cgtccgtgat catgcggagc atctccgcct
     2761 cgcggtcgga gagtccgccc ggaagcacgc cgtcgtccgc atcggtcgtc gggctcgcga
     2821 cctcgaccgg ccccgtcagc tcggcgagcg cgcgctgcgc cggcgctcgg aaccatcgga
     2881 cccgaacgtt ctcgtcggtc gtccgctcgc cgaccatctg caggatcgtt cgcgcgagcg
     2941 tcttcgcctc gtcggcgtcg gggcttccgt cgagcgcccg cagtgcggcg agcacaacgt
     3001 cgagatgcag cgggtacggg gggtcggact cccggaaccc gagcccctcg agggcctgca
     3061 acgcggcgcg agccgatccg cgcgcctcct cgttcctccc gcgggtggca tggaggagcg
     3121 ccatcgcggc ctcggcctga gccgtccagg gatgatcccc acgaagcgac gcggcgagcc
     3181 gacgcgcatc cgcggcccac gcatcggcct cgcggaggag ctcctcgtcg gctccgcccg
     3241 ccgcgagctg cgcggcttcg agcgcgagtc gcgcgaggac ctcgcagcgg ctcgcggcgg
     3301 ttccgcgctc gaccgcgatc tgcagcgctc gctcgaggcg ttctcgcata cccgcgacgt
     3361 cgcccgaggc ggccgccgcc atcccgcgcc acatctcgag gtgtctggcg cggagcgagc
     3421 tcggcgccgc cgcggccgcg gtagtggcgc gctccagcca ctccgtcgct tcggagacct
     3481 cgcccatcgc caggtacgcc agcgccgtac cgcctgcagc gaggaactcg aggtcgcgat
     3541 cccctatccg gagcgcgcca cggtacgcgt cctgccctcg ctcgagcgcg aggtcaggga
     3601 acccgaaggc ccgcgcgtag aggtggacgc cgtacagcat ctggacgtcg tggcgctctc
     3661 gctcgctctc gcggacgagg gtgtgacgcc gagacacgat gcggcgcagg tcctcgatcc
     3721 gacgcgcgga accgaacctg atgtcggggc cggtgcggac gtacgccatc gcgatgagcg
     3781 tcgtcatcat gccgcgctgg tcaccgacgg cctcgtacag ctcgagcgcc ttcccgaatc
     3841 gctcgatcgc gggctggaaa cgctcgggga ggccggggac ggtgaacagg aactcccgcg
     3901 ggggggcctg ccggaggctg gggcgctccg gcgacgccat gaacgcgtag aagtgcttgc
     3961 gagcgcgttc gagcgcgatc gagccgagct cgcgccacgc ggccgcctcc gacgcccggt
     4021 ggccgagctc cgcggcgagc tctgccgctc gctggtacgc ctcctccgcc cgctccagct
     4081 ccgcgtctcc ggcggcggtg ccggcggcct cgccgagggg gctgcgcagg atcgcctggc
     4141 cgagctcgag gcacgcccgc agctccgcct cgtcggcacc tgcggctctc gcacgctcgc
     4201 gggcctgctc cgacagctcg gctgcccgat cctcgtcgcc ggatagccga agcgcggcgg
     4261 cccgccgaag agtcacctcg atcccgagcg cgacgtcgcc gatcgcgtcc gcgagcgcgg
     4321 caagctgcgg aagcgtctcg aggcgctccc gatcgcggcg cagcgcgccg agcgcgtcgt
     4381 cccggagccg aagcagctcc acccggtttc ccgggttcgt cgacgtcgcg agcgactcgt
     4441 cgacgagccg gaggacctcg tcgggcgcgt gaccctcgag ggccgcgcgg gcggcctgca
     4501 ccgagtaccg cgcgcactgc tccccgaggc ccgccgccac cgcgtgatgc gcgaccatgg
     4561 ccatcgcttc cggtggcggg tcgccgccgg agaggaggag gtcgacgatc gccgcgtgga
     4621 tctcccgcct tcgagcgcga ggcagcgacg cagcggcgaa cgacctgatc tgctcgtgcg
     4681 tgaagcggta gtcggcggac catccctcgg ggagctccga aaggaggccc gcggcggcgg
     4741 cgggcgcgag cgatgtcgcg agctcggcga ccgtggtctc gggatcgccg agccgctgtt
     4801 tcaccgacag gagatcggcg aggccgaacg tgcggctgat aaccgccgct tccgcgaggg
     4861 aggttcgcgt cgcgtcgtcg aggcgatcca cacgtcggcg gatcagcatc tcgaccgacc
     4921 gtgggagcag ccgctcggcc ctcggggcga gctgccattc agcgtcgatg cgctgcagca
     4981 gccccgtctc ccggtacgct tgggcgagct cctcgagcat gaacgggacg ccctcggcct
     5041 gtgaatggat cgcctcgagc gtcgacggcg cgaggttccc tccaaggacg tgccgaagca
     5101 gctcaccgct ctcgacgctc ccgaaccgct cgagccgcag ccgccaggcg acgcgcagac
     5161 gctcgaggtc ggcgaggagc gtcgagacct cccgcggcgc ctcggccgtc ggacggagcg
     5221 cgaggacgac gagcacgcgg ctcgtcggct ccgttctcac gatgtagcgg aggagccgga
     5281 tgctgtcctg gtcggcccac tggacgtcgt cgaggaggag tgcgaccggc gcgtgagccg
     5341 cgaggccgcc gagcgtcacg gcggcgaggt cgtaggcgcg gatcagctgc tggtccggag
     5401 ggagcgccat ggccgacgcg tcgggacgtc ccgagagcgc atcgaggagc cggtcgagct
     5461 gctcttcctg tcccgccgct gcggcgagcg ccctcgcggc cgatgacccg aagagcgacc
     5521 gggcgacgag gagcggaccc cgcagctcct cgtcgctgat cgcggtcagg a
//
LOCUS       DASZOB010000295         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116485, whole genome shotgun sequence.
ACCESSION   DASZOB010000295 DASZOB010000000
VERSION     DASZOB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116485"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1114)
                     /locus_tag="VG709_04855"
     CDS             complement(116..1114)
                     /locus_tag="VG709_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC32439.1"
                     /translation="MRPLDFLGSIKLKLGVVILAAVAVTVVVVSLGERTGLSPVVAGV
                     GAALLALAMVQVLAHGMTFPLREMVVAARAMARGDYSRSVTSTSRDEVGELARAFNTM
                     AGELAEVDRMRRDLVANVSHELRTPIGALRATLENLVDEVEPAAPAVLESMLRQTERL
                     GKLAEQLLQLSKLEAGAVPLERRHLPLRTLLLDVADEWRTEAERRDVCFSVRVQPPNL
                     ALHADGDRIRQVLDNLVANAVRHSPVGGTIALSAAAHDGHVQVEVTDEGTGIPPSEAG
                     RVFERFYRSDQARSPSEGGSGLGLAIVRGLVDLHGGTIRVEQAVPTGCRMIVDLPR"
     gene            complement(1131..1814)
                     /locus_tag="VG709_04860"
     CDS             complement(1131..1814)
                     /locus_tag="VG709_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004937733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC32440.1"
                     /translation="MDRRRIAVIEDEAAIAASVAARLRAEGFDVEVSGDGIGGLELCR
                     RFRPDLVVLDLMLPGLDGLEVCRRIQRDRAVPVLMLTARDSETDLEIGLAVGADDYLT
                     KPFSPRELVARVRALLRRAERPLAQDRSPIRIGRLSIDPATRRVARDGHDVHLTPTEF
                     ALLVRLAETPHVVFGRARLLEQVWGHRIPAGERTVDSHVRSLRRKLGHDLIRTVHGIG
                     YSLDTRGLL"
     gene            1838..>2231
                     /locus_tag="VG709_04865"
     CDS             1838..>2231
                     /locus_tag="VG709_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWC32441.1"
                     /translation="MQEASTFRGDLVQGREGARRCHHPVSMTTALDVAGDHEVELEHR
                     VTPLELFFDLVFVFAITQVTGLVSHDPTWESLGRALLVLAALWWAWGAYAWLTNTIDP
                     NEGSARLAVLASMGAMLVAALAVPGAFGE"
BASE COUNT          324 a          790 c          814 g          303 t
ORIGIN      
        1 gatcacgagg cgaagaacgc gatgtcccgg acggtgacga ggagtacgcc gatcgcgacg
       61 ggctggcgcg gtagtagcgc cgccccgacg agcgccgccg tcagcgcggc gagcctcacc
      121 gcggcaagtc gacgatcatc cggcacccgg tcggcaccgc ttgctccacc ctgatggtgc
      181 cgccgtggag gtctacgagc ccgcgcacga tcgcgagccc gagcccgctg ccgccctcgc
      241 tcggcgagcg agcctgatcc gagcggtaga agcgctcgaa cacacgcccg gcctcgcttg
      301 gggggatacc cgttccctcg tccgtcacct cgacctggac gtggccgtcg tgtgccgccg
      361 cgctcagcgc gatcgtccct ccaacaggcg agtggcggac ggcgttggcg acgaggttgt
      421 ccagaacctg tctgattcga tcgccgtcgg cgtggagcgc gaggttcggc ggctgcacgc
      481 ggacgctgaa gcagacgtcc cgccgctcgg cctcggtacg ccattcgtct gcgacgtcga
      541 ggagcagcgt gcgtagcggc aggtgccgcc gctccagcgg cacggcaccg gcctcgagct
      601 tcgagagctg gagcagctgc tcagcgagct tcccgagccg ctccgtctgc cggagcatcg
      661 actccagcac cgccggggcg gcgggctcga cctcgtcgac gaggttctcg agcgtcgcgc
      721 gcagcgcacc gatcggcgtc cgcagctcgt gcgacacgtt ggcgacgagg tcgcggcgca
      781 tgcggtcgac ctcggccagc tcgccggcca tcgtgttgaa cgcgcgcgcg agttcgccga
      841 cctcgtcgcg cgaggtcgaa gtcacgctgc ggctgtagtc gccgcgcgcc atcgcgcgcg
      901 cagcgacgac catctcccgc agcgggaacg tcatcccgtg cgcgaggacc tggaccatcg
      961 cgagcgcgag gagcgcggcg ccgaccccgg ccacgacggg cgagagtccg gtgcgttcgc
     1021 cgagcgagac gacgaccacc gttaccgcga cggcggcgag gatgacgacg ccgagcttca
     1081 gcttgatcga gccgaggaaa tcgagcggcc tcacaggaaa tcgagcggcc tcacaggagt
     1141 ccccgcgtgt cgagcgagta gccgatcccg tgcaccgtgc ggatcaggtc gtggccgagc
     1201 ttgcgccgga gcgagcggac gtgcgagtcg accgtccgct cccccgcggg gatccggtgg
     1261 ccccacacct gctcgagcag ccgggcccgc ccgaatacga cgtgcggcgt ctccgcgagc
     1321 cggacgagga gggcgaactc ggtcggtgtc aggtggacgt cgtgcccgtc ccgcgcgacc
     1381 cgccgcgtcg cggggtcgat cgagagccgg ccgatccgga tcggagagcg atcctgcgcg
     1441 agcggacgct ccgcgcgacg caggagcgcg cgaacgcgcg cgaccagctc acgcgggctg
     1501 aacggcttgg tgaggtagtc gtcggctccg acggcgagcc cgatctcgag gtcggtctcg
     1561 gagtcccggg cggtcaacat gaggaccggc acggcgcggt cgcgctggat gcgccggcac
     1621 acctcgaggc cgtcgaggcc cgggagcatg aggtcgagca cgacgagatc cggccggaag
     1681 cgacggcaga gctcgaggcc gccgatcccg tctcccgaga cctcgacatc gaacccctcg
     1741 gcgcgaaggc gtgccgccac cgacgcggcg atcgccgcct cgtcttcgat gaccgcgatc
     1801 cggcgcctgt ccatcgcccg gagcgtaggg gacgcgcgtg caggaggctt ccacgtttcg
     1861 tggagatctc gtgcagggcc gggaaggcgc gcggcgctgc catcatcccg tctcgatgac
     1921 gacagccctc gacgtcgcgg gggaccacga ggtcgagctc gagcaccgcg tcacgccgct
     1981 cgagctcttc ttcgacctcg tcttcgtgtt cgcgatcacg caggtgacgg gcctcgtctc
     2041 gcacgatccg acctgggaga gcctcggtcg cgcgctgctc gtgctcgcgg cgctctggtg
     2101 ggcgtggggc gcctacgcct ggctgacgaa cacgatcgac ccgaacgagg gctcggcgcg
     2161 cctggcggtg ctcgcgtcga tgggggcgat gctggtggcc gcgctcgccg tcccgggcgc
     2221 gttcggcgag g
//
LOCUS       DASZOB010000296         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_198466, whole genome shotgun sequence.
ACCESSION   DASZOB010000296 DASZOB010000000
VERSION     DASZOB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198466"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VG709_04870"
     CDS             complement(<1..1495)
                     /locus_tag="VG709_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWC32442.1"
                     /translation="MGTVNLYPGRSSFPPIADYAFLSDCETNALVSSAGTVEWMCVPR
                     PDSPSVFGAMLDRGAGSFRIGPDQVMVPAGRRYIPGTMVVETTWMTRSGWINVRDALL
                     IGPWYEQDRREARYRRAPPDHDAEHCLLRTVTCVNGHVEVMLDCEPEFGYGTRRASWS
                     YDGTGYGRATARGDEEDPVLHLVTDTRLGFEGQAARARQTLHEGDGIFAALSWGRRPA
                     PSSFEEAQQRMHVTEDFWRDWLNHGEFPDHPWRWYLQRSALTLKGLTYAPTGALIAAA
                     TTSLPETPRGERNWDYRFSWIRDSTFALWGLYTLGLDAEADDFFFFVADVAEGERDVQ
                     LMYGVGGEKELPEQTLDHLSGYQGARPVRVGNAAYRQEQHDVWGALLDSVYLHAKSRD
                     QLPEHVWPILRQQVENARARWREPDQGIWEVRGPPQHFTSSKLMCWVAADRGARLAEI
                     RGELEQAARWQSAAEEIKADICENAVDERGVFTQHYETDALDASVLLM"
     gene            complement(1495..2328)
                     /locus_tag="VG709_04875"
     CDS             complement(1495..2328)
                     /locus_tag="VG709_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWC32443.1"
                     /translation="MPELPEMQALAERLDAALRGARLIDASPLQFSALKTVAPPVESL
                     AGRALDGVGRRGKYVTFTFGGDRVVVHLSQGGRIDVERPAKRTRPRGGVVRFTFDRGA
                     SLLVKEFGHERRASWWVLRERDDGPLEGLGPEPSSEGFANLVLSSGDRRRVHTLLRDQ
                     RTVAGIGRGYADDILHRARLSPYASLASLAPEERRALLEAVHEVLGEALDEERKRRAG
                     LPPKLGDHFTVHNRYGGPCPHCRDDLRRVSYESHQVTYCPSCQTGGKVLADRRLSRLV
                     R"
     gene            2353..>2642
                     /locus_tag="VG709_04880"
     CDS             2353..>2642
                     /locus_tag="VG709_04880"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HWC32444.1"
                     /translation="MPSRRRDEPWRPAAADLEAARERTVPDVVAPGLRVLFVGINPGL
                     YSAAIGHHFGRPGNRFWPALHASGFTDRLLSPWEEGELLAVGIGITNVVPRAT"
BASE COUNT          365 a          964 c          942 g          371 t
ORIGIN      
        1 gcatgaggag gaccgaggcg tcgagcgcat ccgtctcgta gtgctgggtg aacaccccgc
       61 gctcgtcgac cgcgttctca cagatgtcgg ccttgatctc ctccgccgcc gactgccagc
      121 gcgccgcctg ctcgagctcg ccccggatct ccgccagccg ggcgccgcgg tcggctgcga
      181 cccagcacat cagcttggac gacgtgaagt gctgcggcgg gcctcggacc tcccagatgc
      241 cctggtccgg ctcacgccac cgcgcgcgtg cgttctcgac ctgctgacgg aggatcggcc
      301 acacgtgctc cgggagctgg tctcgcgact tcgcgtggag gtagacggag tcgaggaggg
      361 ctccccagac gtcgtgctgc tcctggcggt aggcggcgtt tccgacgcgg acgggccgcg
      421 cgccctggta gccggagagg tggtcgagcg tctgctccgg cagctccttc tcgccgccga
      481 cgccgtacat gagctgcacg tcgcgctccc cctccgccac gtcggccacg aagaagaaga
      541 agtcgtcggc ctcggcgtcg agcccaagcg tgtacaggcc ccacagcgcg aacgtggagt
      601 cgcggatcca gctgaagcgg tagtcccagt tccgctctcc gcggggggtc tccggcagcg
      661 acgtggtggc cgcggcgatc agcgccccgg tcggcgcgta cgtgaggccc ttgagcgtca
      721 gcgcgctccg ctgcaggtac cagcgccagg gatggtcggg gaactcgccg tggttgagcc
      781 agtcccgcca gaagtcctcg gtgacgtgca tgcgctgctg cgcctcctcg aacgaggacg
      841 gcgcgggacg cctcccccaa gagagcgccg cgaagatccc gtcgccctcg tggagcgtct
      901 ggcgcgcgcg cgccgcttgc ccctcgaacc cgaggcgcgt gtcggtcacg aggtgcagga
      961 ccgggtcctc ctcgtcgccg cgtgccgtgg cgcgcccgta ccccgttccg tcgtacgacc
     1021 acgacgcgcg cctcgtcccg tacccgaact ccggctcgca gtcgagcatc acctcgacgt
     1081 gcccgttgac gcacgtgacg gtccggagca ggcagtgctc ggcgtcgtgg tcgggcggcg
     1141 ctcgccggta ccgcgcctcc cgccggtcct gctcgtacca cgggccgatg agcaatgcgt
     1201 cgcgcacgtt gatccagccg ctgcgagtca tccacgtcgt ctcgacgacc atcgtcccgg
     1261 ggatgtagcg acgccccgca gggaccatga cctggtcggg cccgatacgg aacgaccccg
     1321 cgccgcgatc gagcatcgcg ccgaagacgc tggggctgtc gggtcgcggg acgcacatcc
     1381 attcgacggt cccggccgac gagaccagcg cgttcgtctc acagtcggac aggaacgcgt
     1441 agtccgcgat cggagggaac gagctccgtc ccggatagag gttgacggtg cccatcagcg
     1501 gaccagccgg gaaagcctgc ggtcggcgag aaccttcccg ccggtctgac acgacgggca
     1561 gtacgtgacc tggtgcgact cgtacgagac gcgccggagg tcgtcgcggc agtgagggca
     1621 cggcccgccg tagcggttgt ggaccgtgaa gtggtcgccg agcttcggcg gcaggccggc
     1681 gcggcgcttc cgctcctcgt cgagcgcttc gccgagcacc tcgtggacgg cctcgagcag
     1741 cgcccgtcgc tcttcgggcg ccagcgacgc gagcgacgcg tacggggaga ggcgcgcgcg
     1801 gtgcaggatg tcgtccgcgt agccgcggcc gatgccggcg accgtcctct ggtcgcgcag
     1861 gagcgtgtgg acgcgtcggc gatcgccgga cgagagcacg aggttcgcga acccctcgga
     1921 ggacggctcg gggccgaggc cctcgagcgg cccgtcgtcc ctctcacgca gcacccacca
     1981 ggaggcccgc cgctcgtgac cgaactcctt cacgagcagc gacgcgccgc gatcgaacgt
     2041 gaaccgcacg acgccgccgc gcggacgcgt ccgcttcgcc ggccgctcca cgtcgatgcg
     2101 cccgccctgc gagaggtgca cgacgacgcg gtcgccgccg aacgtgaacg tcacgtactt
     2161 gccccggcgc ccgacgccgt cgagcgcgcg gccggccaag gactccaccg gcggcgcgac
     2221 cgtcttgagc gccgagaact gcaggggcga ggcgtcgatc agccgcgcac cgcggagcgc
     2281 cgcatccagc cgctccgcga gcgcctgcat ctccggcagc tccggcacgg gagcgagtgt
     2341 agcgtcggag gcgtgccgag ccgccgtcgc gatgagccgt ggcgccccgc ggcggcggac
     2401 ctcgaggccg cgcgcgaacg cacggttccg gacgtggtcg cgccggggct ccgcgtgctg
     2461 ttcgtcggga tcaaccccgg cctctactcc gcggcgatcg gccaccactt cggtaggccg
     2521 ggcaaccggt tctggcctgc gctccacgcg tccgggttca ccgatcggct gctgtctccg
     2581 tgggaggagg gcgagctgct cgcggtcggg atcgggatca cgaacgtcgt gccgcgcgcg
     2641 ac
//
LOCUS       DASZOB010000297         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116546, whole genome shotgun sequence.
ACCESSION   DASZOB010000297 DASZOB010000000
VERSION     DASZOB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116546"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VG709_04885"
     CDS             complement(<1..188)
                     /locus_tag="VG709_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWC32445.1"
                     /translation="MADDRPKVLLVEDDESVRQLVRVTLQMNDFDVVEAKDGLEGLLL
                     LDMHKPDAVVLDLMMPDVG"
     gene            complement(191..316)
                     /locus_tag="VG709_04890"
     CDS             complement(191..316)
                     /locus_tag="VG709_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32446.1"
                     /translation="MIAAFVTLEIGFLIFVGAMTGLAGLFALYVLIQHFRNPSRR"
     gene            393..1142
                     /locus_tag="VG709_04895"
     CDS             393..1142
                     /locus_tag="VG709_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWC32447.1"
                     /translation="MGEGYKPFYWTMKYVVLGPLLRGVYRPRAVGVENIPATGPVILA
                     SNHVSFVDSLFMPLMCPRPVVFLGKADYFESWRTRWFFDALNVIPVRREGGSASEAAI
                     LAGIRALKRGHVIGIYPEGTRSPDGRLYRGKTGVARMALEAQAPVVPIAVEGTDVVMP
                     LDAKMPRLKGRPAVTYGKPLTFERYYDRPKDRFVLRSVTDEIMYEIMMLSGQEYVDEY
                     ASKVKAQLAKQGSAPEEPAAKPQPAEPSNLT"
     gene            1189..1488
                     /locus_tag="VG709_04900"
     CDS             1189..1488
                     /locus_tag="VG709_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32448.1"
                     /translation="MQQLTEMMAARAGTHASAPYDRVIAVIHEAPEEQLPPLIDQLRQ
                     GVADLEKVVEAAPPVAVSDETDGDIAVRPRETEYDDWVRRELGGDSEGPPGFTVL"
     gene            complement(1475..>2292)
                     /locus_tag="VG709_04905"
     CDS             complement(1475..>2292)
                     /locus_tag="VG709_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWC32449.1"
                     /translation="VLGARVPIAGIAGDQQAALVGQACFAAGDAKNTYGTGSFVLLNT
                     GTDSASVPGVLTTVAYSDAEGVRYALEGSIFVTGAAIQWLRDGLGIIGAAAEAGPRAE
                     TVADAGGVYLVPALVGLGSPWWDPYARGTIVGITRGTTRAHLVRAAVEAMAYQTRDVV
                     DAMVRGGGIPLRTLKIDGGASAMDLLGQLQADLLDVRVLRPQVQETTGMGAAFLAGLQ
                     SGVWSTKEEAASAWRLDREFAPRDREGAERRYAGWRRAVERSLRWEEPAPTGP"
BASE COUNT          372 a          801 c          797 g          322 t
ORIGIN      
        1 ccgacgtcgg gcatcatgag gtcgagcacc acggcgtcgg gcttgtgcat gtcgaggagc
       61 agcagcccct cgagcccgtc cttcgcctcg accacgtcga agtcgttcat ctggagcgtg
      121 accctgacga gctgtcggac cgactcgtcg tcctcgacca gcaggacctt cgggcgatca
      181 tcggccaccg ctacctccgt gaggggttcc ggaagtgctg gatgagcacg tacagcgcga
      241 agagacccgc gaggccggtc atcgcgccga cgaagatcaa gaacccgatc tcgagcgtaa
      301 cgaacgcagc gatcatcgat cctccggtga cttgaacgcc cgaagtcgag tctactaggg
      361 tgaatgcccg cggcgtgagt tgggaggccg cgatgggtga gggttacaaa ccgttctact
      421 ggacgatgaa gtacgtcgtc ctcgggccgc tgctgcgcgg cgtctaccgt ccgcgcgccg
      481 tgggggtgga gaacatcccg gccaccgggc cggtgatcct cgcgtcgaac cacgtgtcgt
      541 tcgtggacag cttgttcatg ccgctcatgt gcccccgtcc ggtcgtgttc ctgggcaagg
      601 ccgactactt cgagagctgg cgaacacgct ggttcttcga cgccctgaac gtcatcccgg
      661 tccggcgcga gggcggctcc gccagcgagg ccgcgatcct cgccgggata cgagcgctga
      721 agcggggaca cgttatcggt atctacccgg aaggaacgcg gagccccgac ggccggctct
      781 accgcggcaa gaccggagta gcgcggatgg ccctcgaggc acaggccccc gtcgtgccga
      841 tcgcggtcga gggcaccgac gtcgtgatgc cgctcgacgc gaagatgccg cgcctcaagg
      901 ggcgccctgc ggtcacgtac ggcaagccgc tcacgttcga gcggtactac gaccgaccga
      961 aggaccggtt cgtgctgcgg tccgtcaccg acgagatcat gtacgagatc atgatgctct
     1021 cggggcagga gtacgtcgac gagtacgcct cgaaggtgaa ggctcagctc gcgaagcagg
     1081 gatccgcgcc cgaggagccc gcggccaagc cgcagccggc ggagccgtcc aatctcacct
     1141 gaaggctccg acgccccgcg ggaagaggcg gagcggctga tccgccgcat gcagcagctg
     1201 accgagatga tggccgcgcg cgccggcacg cacgcgagcg cgccgtacga ccgcgtgatc
     1261 gcggtgatcc acgaggcgcc cgaggagcag ctgccgccgc tcatcgacca gctccgccag
     1321 ggcgtcgcgg acctcgagaa ggtcgtggag gccgcgccac ccgtggccgt ctccgacgag
     1381 acggacggag acatcgcggt gcggccgcgc gagaccgagt acgacgactg ggtgcgacgc
     1441 gagctcgggg gcgactcgga aggccctccc ggcttcacgg tcctgtagga gccggctcct
     1501 cccaccgcag cgaccgctcg acagctcgcc gccatcccgc gtagcggcgc tcggccccct
     1561 cgcgatcgcg aggagcgaac tcgcggtcga gacgccaggc cgacgctgct tcctctttcg
     1621 tcgaccagac gcccgactgc agccccgcga ggaaggccgc ccccatcccg gtggtctcct
     1681 ggacctgcgg gcgcagcact cggacatcga ggagatcggc ctgcagctga ccgagcagat
     1741 ccatcgcgct cgcccctccg tcgatcttca gcgtgcggag ggggatccct ccgccacgga
     1801 ccatcgcatc gacgacgtcc cgcgtctggt acgccatcgc ctcgacggcg gcgcggacga
     1861 gatgcgcgcg cgtggtgccg cgcgtgattc cgacgatcgt gccgcgcgcg tacgggtccc
     1921 accagggcga ccccaggccg acgagcgcgg gcacgaggta cacgccgccg gcgtcggcga
     1981 cggtctcggc gcgcggtccg gcttccgccg cggcgccgat gatgccgagg ccgtctcgga
     2041 gccactggat cgcggcgccg gtcacgaaga tcgagccctc gagcgcgtac cgtacgccct
     2101 cggcgtcgga atacgcgacg gtcgtcagaa cccccggcac ggaggcgctg tccgtgccgg
     2161 tgttgagcag gacgaacgac cccgtcccgt acgtgttctt ggcgtcgccg gcggcgaagc
     2221 atgcctggcc gacgagcgcg gcctgctgat cgccggcgat gccggcgatt gggacgcgcg
     2281 cgccgaggac cg
//
LOCUS       DASZOB010000298         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_67306, whole genome shotgun sequence.
ACCESSION   DASZOB010000298 DASZOB010000000
VERSION     DASZOB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67306"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1130
                     /locus_tag="VG709_04910"
     CDS             207..1130
                     /locus_tag="VG709_04910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWC32450.1"
                     /translation="MPIQPVDKIWMDGKLLDWEDAQVHVLTHALHYGSGVFEGIRAYE
                     TERGAAIFRLTDHIRRLFKSAAVYMMDIGFSIEELVAASKETVRENGLKSCYVRPIVY
                     RGYGEMGLNPIPAPVNVAIAVWPWGTYLGQDCIENGARLMISSWRRPDPNVLPAQAKA
                     TGQYINSGLAKVEAVHAGFDDALMLGPDGYVAEATGENLFVIRDEKVFTPPEASGVLL
                     GVTRDAVMRIATDLGYEVVERKLTRADVYMADEAFLTGTAAEVTPVREVDNRPLGEGG
                     RGPITKDIQQTYFSAVKGQIEKYDEWCEYVD"
     gene            1149..1910
                     /locus_tag="VG709_04915"
     CDS             1149..1910
                     /locus_tag="VG709_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWC32451.1"
                     /translation="MRLVRVRYGDRIAAGVLEGDVVRPLRGTFFEEPIPTGEEIPVDD
                     VRLLAPVIPSKVVGVARNFAAHAEELGNPVPDEPILFFKPATSVIGPEDPIPLHPESE
                     RIDHEAEVAAVIGRLCRRVSEEDADRYILGYTCANDVTARDLQKRDGQWARAKGSDGF
                     CPLGPWIETEIDASDLEIVARVGDDVRQKGRTSDMKFGAATLVAFASRTITLLPGDVI
                     LTGTPPGVAPLGAGDRVDVDVEGIGVLRNPVVAGG"
BASE COUNT          313 a          645 c          691 g          280 t
ORIGIN      
        1 tgtgccgagg aaccggaccg cggcaaccac cgcgccgacg gcgatgagcg cagccgcgag
       61 ccaggtgaac gggatgggac gacgggttgc gacgccggga tgatagcgca ggggacgggc
      121 cctccagcgt atggttcccg ccctcgcggt cacgcccgcc gggcccggtg tacgctttgc
      181 gtcggtcacc gaacggggga gggcagatgc cgattcagcc ggtcgacaag atctggatgg
      241 acgggaagct cctcgactgg gaagacgccc aggtccacgt cctgacccac gcgctccact
      301 acgggagcgg cgtcttcgaa gggatccgcg cgtacgagac agagcgcgga gccgcgatct
      361 tccgtctcac ggatcacatc cggcgcctgt tcaagtcggc cgccgtctac atgatggaca
      421 tcgggttctc catcgaggag ctcgtcgcgg cctcgaagga aaccgtccgg gagaacggcc
      481 tcaagtcgtg ttacgtgcgt ccgatcgtgt accgcggcta cggcgagatg ggactgaacc
      541 cgatccccgc gccggtgaac gtcgcgatcg cggtgtggcc gtgggggacg tatctcggcc
      601 aggactgcat cgagaacggc gcccgcctca tgatctcgtc gtggcggcgt ccggacccga
      661 acgtcctccc ggcgcaggcg aaggcgaccg gccagtacat caactcgggt ctcgcgaagg
      721 tcgaggccgt acacgccggg ttcgacgacg cgctgatgct cgggcccgac ggctacgtcg
      781 ccgaggcgac cggcgagaac ctgttcgtga tccgcgacga gaaggtcttc accccgcccg
      841 aggcgtcggg cgtgctcctc ggcgtcacga gggacgccgt catgcgcatc gcgaccgacc
      901 tcggttacga ggtcgtagag cggaagctga cgcgcgcgga cgtgtacatg gccgacgagg
      961 cgttcctcac ggggaccgcc gcggaggtca cgccggtccg agaggtcgac aaccggccgc
     1021 tcggcgaagg cggacgcggc ccgatcacga aggacatcca acagacgtac ttctcggcgg
     1081 tgaagggcca gatcgagaag tacgacgagt ggtgcgagta cgtcgactga cggctcgttg
     1141 atcgccgcgt gaggctggtc cgcgtccgct acggcgaccg catcgcggcc ggagtcctcg
     1201 agggcgacgt cgtccgaccg ctgcgcggca cgttcttcga ggagccgatc cccacggggg
     1261 aggagatccc ggtcgacgac gtccggctcc tcgcgccggt gattccgtcg aaggtcgtcg
     1321 gcgtcgcgcg gaacttcgcg gcccacgccg aggagctcgg caaccccgtc cccgacgagc
     1381 cgatcctgtt cttcaagccc gcgacctcgg tgatcgggcc ggaggatccg atcccgctcc
     1441 atcccgagtc ggagcgcatc gatcacgagg ccgaggtcgc cgccgtcatc ggccgcctgt
     1501 gccgccgcgt gagcgaggag gacgcggaca ggtacatcct gggctacacc tgcgccaacg
     1561 acgtcacggc gcgagacctg cagaagcgcg acgggcagtg ggcgcgcgcg aaggggtccg
     1621 acggcttctg cccgctcggg ccttggatcg agacggagat cgacgcttcg gacctcgaga
     1681 tcgtcgctcg tgtcggcgac gacgtccggc agaagggacg gacgtccgac atgaagttcg
     1741 gtgcggcgac gctcgtggcc ttcgcctcgc ggacgatcac gctccttccc ggagatgtga
     1801 tcctgaccgg cacgccgccc ggcgtcgcac cgctcggcgc cggcgatcgc gtcgacgtcg
     1861 acgtcgaagg gatcggcgtg ctgcgtaacc cggtcgtcgc cggtggctga gcgctcggtc
     1921 cgcgtgcgg
//
LOCUS       DASZOB010000299         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_157649, whole genome shotgun sequence.
ACCESSION   DASZOB010000299 DASZOB010000000
VERSION     DASZOB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157649"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VG709_04920"
     CDS             <1..655
                     /locus_tag="VG709_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWC32452.1"
                     /translation="DGEAVDYDTFAAAVDENVGRPISITVRRGGSEVTVEATPVLAEV
                     EGEETGRLGISYRQGGVLARHRLSPIEALGGSAAGVGELVVASFRAMGRVFGPEGLGR
                     IGEVLVGGRERSLNDPVGVVGAARVAGQAVQAGFFDVFLQLFAVFNVFVGLLNLMPLP
                     PLDGGHLAVLGFEAVTRRPVDPRRLVPLTALVAGFLVLFTVALLYIDIANPLPNIFQ"
     gene            677..1774
                     /gene="ispG"
                     /locus_tag="VG709_04925"
                     /gene_synonym="gcpE"
     CDS             677..1774
                     /gene="ispG"
                     /locus_tag="VG709_04925"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWC32453.1"
                     /translation="MISERRSSRKVRVGWVEVGGDAPVRVQSMTTTKTADVNATLQQM
                     AALVATGCEIVRVAVPHKEDAEALRQIAEHATIPVVADIHFQWRYAMMALEAGIHGLR
                     INPGNIKYQDKVRLIAREAKARGVPIRIGVNAGSLEPSLLERYGRPTAEALVESAIDE
                     VRILEDEDFNDIKISVKHSDTAEMIKAYRMLAEKVDYPLHLGVTEAGPMPQGGIKSAV
                     GIGTLLAEGIGDTIRVSLTDDPVDEVKVGNWILQSLGLKKRGLDLVACPSCGRAEVDV
                     LGLTKQVNEALEREGIKAPIRVAVMGCVVNGPGEAREADLGIAAGKGYGQLIVKGEVQ
                     ATRVPESRFVEVLVDEAKKLAAERESEAATT"
     gene            1909..>2336
                     /locus_tag="VG709_04930"
     CDS             1909..>2336
                     /locus_tag="VG709_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HWC32454.1"
                     /translation="MRRHVVTLAITATVVLATSIAFAVPGDLDTTFGGGDGKVTTDFD
                     ADVALYDAARDVAAAPGGRIVAVGVGQAPNYDFAIARYLPNGDLDTSFGGDGKVTTDF
                     FGQPDWAYAVEILPDGKILVVGSARSADNSDDFFALARYRT"
BASE COUNT          365 a          778 c          850 g          343 t
ORIGIN      
        1 cgacggcgag gccgtcgact acgacacgtt cgcggccgcc gtcgacgaga acgtcggtcg
       61 cccgatctcg atcacggtcc gtcgcggcgg gtccgaggtg acggtcgagg cgacgcccgt
      121 cctcgcggag gtcgaaggcg aggagaccgg gcggctcggg atcagctacc ggcagggcgg
      181 cgtcctcgcg cggcacaggc tgtcgccgat cgaggcactc ggcggctccg ccgcgggtgt
      241 gggcgagctc gtcgtggcgt cgttccgggc gatgggtcgc gtgttcgggc ccgaggggct
      301 cggccgcatc ggcgaggtcc tcgtcggcgg gcgcgagcgc tcgctcaacg accccgtcgg
      361 ggtcgtcggc gccgcacgcg tcgccgggca ggcggtccag gcggggttct tcgacgtgtt
      421 cctgcagctg ttcgctgttt tcaacgtctt cgtcgggctg ctcaacctga tgccgcttcc
      481 gccgctcgac ggcggtcacc tcgcggtgct cgggttcgag gccgtcacgc gacgcccggt
      541 cgacccccgc cggttggttc cgctgacggc gctcgtcgcc gggttcctcg tgctgttcac
      601 ggtcgcgctg ctctacatcg acatcgcgaa ccccctgccg aacatcttcc agtagcgccg
      661 tcgatacact gggtgggtga tctcggaacg gcggagctcc cggaaggtcc gcgtcggctg
      721 ggtcgaggtc ggcggcgacg cgcccgtgcg cgtgcagtcg atgaccacga cgaagaccgc
      781 cgatgtgaac gcgacgctgc aacagatggc ggcgctcgtc gcgacggggt gcgagatcgt
      841 gcgcgtcgcg gtgccgcaca aggaggacgc cgaggcgctc cgacagatcg cggagcacgc
      901 gacgatcccg gtcgtcgccg acatccactt ccagtggcgc tacgcgatga tggcgctcga
      961 ggccgggatc catggactcc gcatcaaccc cggcaacatc aagtatcagg acaaggtgcg
     1021 gctgatcgcg cgcgaggcga aggcgcgcgg cgtcccgatt aggatcggcg tcaacgcggg
     1081 ctcgctcgag ccgtcgctgc tcgagcggta cggtaggccg accgccgagg cgctcgtgga
     1141 gtccgcgatc gacgaggtcc gcatcctcga ggacgaggac ttcaacgaca tcaagatctc
     1201 ggtgaagcac tccgacaccg ccgagatgat caaggcgtac cggatgctcg ccgagaaggt
     1261 cgactacccg ctccacctcg gcgtcaccga ggcgggcccg atgccgcagg gcggcatcaa
     1321 gtccgccgtc gggatcggca cgctgctcgc ggaggggatc ggcgacacga tccgcgtgtc
     1381 gctcaccgac gaccccgtag atgaggtgaa ggtcgggaac tggatcctcc agtcgctcgg
     1441 gctcaagaag cgcgggctcg acctcgtcgc atgcccctcg tgcggccgcg cggaggtcga
     1501 cgtgctcggc ctcacgaagc aggtcaacga ggcgctcgag cgcgagggca tcaaggcccc
     1561 gatccgcgtc gcggtcatgg gatgcgtcgt caacggcccc ggcgaggcgc gcgaggccga
     1621 cctcgggatc gcggccggga agggctacgg acagctgatc gtgaagggcg aggtgcaggc
     1681 gacgcgcgtc ccggagtcgc gcttcgtcga ggtgctcgtc gacgaggcga agaagctggc
     1741 cgcagagcgg gagtccgagg ccgccaccac gtaacccacg cacgacgtca ttacggacga
     1801 gcgggacaac ctcgtccgat gccattgacc ggagatccaa cgagtccgta cggtggcgac
     1861 gcgttcgcgc gccgtgttag cgccgcgaac ccatggggga gggagctcat gcgacgtcac
     1921 gtagtgacgc ttgccatcac ggcaacggtt gtcttggcaa cgtcgatcgc gttcgccgta
     1981 ccgggcgacc tcgacacgac gttcgggggc ggcgacggga aggtcacgac cgatttcgac
     2041 gcggatgtcg ccctgtacga cgcggcgcgc gacgtcgccg cggcgccggg cggcaggatc
     2101 gtcgcggtcg gcgtcggcca ggctccgaac tacgacttcg cgatcgcgcg gtacctgccg
     2161 aacggcgatc tcgacacgag cttcggcggc gacggcaagg tgacgacgga cttcttcggg
     2221 cagcccgact gggcgtacgc cgtcgagatc ctcccggacg gaaagatcct cgtcgtcggc
     2281 agcgcgagga gcgccgacaa cagcgacgac ttcttcgcgc tcgcgaggta ccggac
//
LOCUS       DASZOB010000300         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116587, whole genome shotgun sequence.
ACCESSION   DASZOB010000300 DASZOB010000000
VERSION     DASZOB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116587"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VG709_04935"
     CDS             complement(<1..469)
                     /locus_tag="VG709_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWC32455.1"
                     /translation="MPSLRIVLPTEDLRPGLDEIRREFDVPGAFPPEVEQEAERAAAS
                     PAGDDERRDARDLPRLTIDPPGSRDLDQAFGALRAGRGYRVHYAIADLTPFVRPGGAV
                     DAETWRRGETGYMPDARAPLHPRVLSEGAASLLPAEDRPAVLWTIDLDERGEPA"
     gene            complement(479..>2137)
                     /locus_tag="VG709_04940"
     CDS             complement(479..>2137)
                     /locus_tag="VG709_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWC32456.1"
                     /translation="YRVHRRYGEDTNVLETRFETDDGVLVVTDCLAVHPGVSARDSRR
                     SAEPYHALIRIARCEDGEVDARLEFEPRFDYGLTVPRLERHGRHVAAVYGGADALLLD
                     STLPVEQVGTCSAIASGRLRRGETATVSIRYYLPNELRIDPLDAEESARRMEETIEFW
                     RDWAATCTDDGASRGAVMRSALVLKALTNAPTGAIVAAPTTSLPEHVGGHRNWDYRYT
                     WLRDAALNLYALFELGYTGEAHDFMTWVKRTTAGRAEDLQIMYGVGGERLLHEAELDL
                     EGYRASRPVRIGNAAFSQFQLDIYGELLDTAWLYHRHGGEIDEVFWELCRGVVDVVQE
                     RWTEPDHGIWEQRTAERHYVHSKVMAWVAVDRAIRLARALGLPADLDRWRALRRDIRE
                     RVEHEGVDAETGAFVRAFGGDAVDASNLLFPLVRFLKADDPRVRATFERVEKELTRGG
                     LVDRFHLEDGRPRDEGTFGICSFWLVDNLALAGERDRAVELFERLLDHANDVGLLSEE
                     IDPHTGELLGNFPQAFSHVGLIGAALNLAKAVEGSRGENEPGAG"
BASE COUNT          299 a          802 c          730 g          306 t
ORIGIN      
        1 acgccggctc gccgcgctcg tcgaggtcga tcgtccacag caccgccggt ctgtcctccg
       61 ccgggagcag gctcgccgcc ccctcggaga gcacgcgcgg gtgcagcggc gcccgggcgt
      121 ccggcatgta gccggtctcg ccgcgacgcc acgtctcggc atcgaccgcg ccccccggac
      181 ggacgaacgg cgtcaggtcc gcgatcgcgt agtggacgcg gtagccgcgc ccggcccgga
      241 gcgccccgaa cgcctggtcg aggtcccggc tgccgggcgg gtcgatcgtc agccgcggca
      301 ggtcgcgcgc gtcacgtcgc tcgtcgtcgc cggccggtga cgccgcggcc cgttcggcct
      361 cctgctcgac ctcgggcggg aacgcgcccg ggacgtcgaa ctcgcggcgg atctcgtcga
      421 gccccggacg caggtcctcg gtcggcagga cgatccgcag cgacggcacg cgaggacgct
      481 acccggctcc cggctcgttc tcccctcgcg atccctcgac cgctttcgcc aggttgagag
      541 ccgcaccgat cagcccgacg tggctgaacg cctgggggaa gttcccaagg agctccccgg
      601 tgtgagggtc gatctcctcg gagagcaagc cgacgtcgtt cgcgtggtcc agcaggcgct
      661 cgaacagctc gacggcgcgg tcgcgctcac ccgccagcgc gaggttgtcc accagccaga
      721 acgagcagat cccgaacgtc ccctcgtcgc gggggcgtcc gtcctcgagg tggaacctgt
      781 ccacgagccc tccacgcgtc agctccttct cgacgcgctc gaacgtcgcg cggaccctcg
      841 ggtcgtccgc cttcaggaac cgcacgagcg ggaagagcag gttcgacgcg tccacggcat
      901 ccccgccgaa cgcgcgaacg aacgcaccgg tctcggcgtc gacaccctcg tgctcgaccc
      961 gctcgcggat gtcgcggcgg agcgctcgcc agcggtcgag atccgcaggc agtccgagcg
     1021 cgcgtgcgag ccggatcgcg cggtcgaccg ccacccaggc catcaccttc gagtgcacgt
     1081 agtgacgctc ggccgtgcgc tgttcccaga tcccgtggtc cggctcggtc cagcgctcct
     1141 ggacgacgtc cacgacgcca cgacagagct cccagaacac ctcgtcgatc tccccgccgt
     1201 ggcggtggta gagccacgcg gtgtcgagca gctcgccgta gatatccagc tggaactgcg
     1261 agaaggccgc gttcccgatc cggacgggcc gcgatgcccg gtacccctcg aggtcgagct
     1321 cggcctcgtg cagcagccgt tcccctccga ctccgtacat gatctggagg tcctcagcgc
     1381 gccccgcggt cgtccgcttg acccacgtca tgaagtcgtg tgcctcgccg gtgtagccga
     1441 gctcgaacag cgcgtagagg ttgagcgccg cgtcgcgcag ccacgtgtag cggtagtccc
     1501 agttccgatg gccacccacg tgttcgggga gcgaggtcgt gggcgcggcg acgatcgccc
     1561 cggtcggtgc gttcgtgagc gccttcaaga cgagcgcgct ccgcatgacc gcccctcggg
     1621 aggcgccgtc gtccgtgcac gtggcggccc agtctcgcca gaactcgatc gtctcctcca
     1681 tccgacgcgc cgactcctcg gcgtccagcg gatcgatccg gagctcgttg gggaggtaat
     1741 agcggatgct gaccgtggcc gtctcgccgc gccgcagccg gccgctcgcg atcgcggagc
     1801 acgtccccac ctgctccacc ggcaacgtcg agtcaaggag gagggcatcc gcgcctccgt
     1861 acacggccgc gacgtgccgc ccgtgccgct ccagccgcgg gacggtgagc ccgtagtcga
     1921 agcgaggctc gaactccagg cgggcgtcga cctcgccgtc ctcgcaccgc gcgatgcgga
     1981 tcaaggcgtg gtacggctcc gcggaccggc ggctgtcacg cgcggagacg ccgggatgca
     2041 ccgcgaggca gtcggtcacg acgaggacgc cgtcgtcggt ctcgaaccga gtctcgagga
     2101 cgttcgtgtc ctcgccgtac cggcggtgca cccggta
//
LOCUS       DASZOB010000301         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116592, whole genome shotgun sequence.
ACCESSION   DASZOB010000301 DASZOB010000000
VERSION     DASZOB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116592"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VG709_04945"
     CDS             <1..1401
                     /locus_tag="VG709_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32457.1"
                     /translation="VLERLNRRLPMLTGGARNVPERQRTLRSTIAWSEELLEPPHRAV
                     FARLSVLLGDWTLEAAEAVANPGSEAGVETFDALEALHDHALVRPREDGEGGPWFSML
                     ETIREYALERLASSGEEDVVRRRHAAHFLELAEAGERELTGARTVEWRRRLDREHPNL
                     RAAVEWSLESGDAETGLRISAALWRLWQLRGTLREGVEVMKGLLQLPGSRPRTAIRAK
                     ALIAAGSIAYWAGDLASARERYEEALSISRDLGDEVGIAHALYNVAFTHALFDEHGLA
                     RATFEDAQRRFEVLDDPLMTAHAKLARAMVALRAPDQGDPEPLAAEALRVFEELGAPH
                     SITLAHGILAQIAFVRGDEAPMIRHTLRSLEVSQAVDDPLGTAVQLDVVAAVLGRRGH
                     HERALRLAGASRRIKDDAGGEAPRALIEVEDARTLAAGALSEEQIRALLEEGGQIGAE
                     DAAELARRELEALAPG"
     gene            1398..>2644
                     /locus_tag="VG709_04950"
     CDS             1398..>2644
                     /locus_tag="VG709_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32458.1"
                     /translation="MTARLARALRVRAGEGPVVLPLLALMLVAWAGFAIGANGVESIF
                     FTRFGPQFLPWMYIALALVTVPATLGVGAVLGRGDRRRVVAAFAPAMAVAVFALRGLA
                     VASSAWVYPTLWLGMMVLWIVQGIAVWSVAGVVHDARQAKRLFPLYGAGVILGGALGG
                     LATPPLAATIGAENLLLVWGASLVAAWPLCRVVLAAPARHRHPALRGHRPRPPRVLEE
                     MREGFRTARSSPLLRRMSLAAVLVGFLYFTLTLPFAEAVTARYPDTDRLASFLGLFSG
                     TANALALVVSLAAANRLFARFGVPAMVIVMPVVYLLGFGVVAVSASFATLIAFRLVQL
                     VWVHGVYTTGWQALFGIVPPERRAQARTFVEGGPMQLGILISGAVLLLAQRLIADRTL
                     FVVAAAVAAAAVLVCWWLRRAYG"
BASE COUNT          298 a          907 c         1010 g          429 t
ORIGIN      
        1 gtcctggagc ggctgaaccg gcggctgccg atgctcacgg gtggcgctcg caacgtgccg
       61 gagcgacagc ggacgcttcg ttcgacgatc gcgtggagcg aggagctcct cgagccgccg
      121 caccgggccg tgttcgcgcg cctctcggtc ctcctcggcg actggacgct cgaggccgcc
      181 gaggcggtcg cgaatcccgg ttcggaggcg ggtgtcgaga cgttcgacgc gctcgaggcg
      241 ttgcacgacc acgccctcgt gcgacctcgg gaggacggcg agggaggacc gtggttctcg
      301 atgctcgaga cgatccgcga gtacgcgctc gagcgactcg cttcttccgg cgaagaggac
      361 gtcgtccgca ggcgccacgc cgctcacttc ctcgagctcg cggaagccgg cgaacgggag
      421 ctcacgggcg cacggacggt cgaatggcga cggcgtctcg atcgcgagca cccgaacctc
      481 cgcgcggccg tcgagtggtc gctcgagagc ggcgatgcgg agaccgggct ccgcatcagc
      541 gccgcgctct ggcgcctgtg gcagctgcgg gggacgctgc gcgaaggcgt cgaggtgatg
      601 aagggcctgc tccagctccc gggctcgcga ccgcgcacgg ccatccgcgc caaggcgctc
      661 atcgcggccg gcagcatcgc gtactgggcg ggcgacctcg cgagcgcgcg tgagcgttac
      721 gaggaggcgc tttcgatctc gcgcgacctc ggagacgagg tcgggatcgc tcacgccctg
      781 tacaacgtcg cgttcacgca cgcgctgttc gacgagcacg ggctcgcgag ggcgacgttc
      841 gaggatgcgc agcgccgatt cgaggtcctc gacgatccgt tgatgaccgc gcacgcgaag
      901 ctcgcgcgcg cgatggtggc gctgcgcgcg ccggatcagg gcgaccccga gccgctcgcc
      961 gccgaggcgc tccgggtgtt cgaggagctt ggggcgcctc attcgatcac gctggcgcac
     1021 gggatcttgg cgcagatcgc gttcgtacgt ggggacgagg cgcccatgat ccgccatacg
     1081 ctgcggtccc tcgaggtctc gcaggcggtg gacgacccgt tggggacggc ggtccagctc
     1141 gacgtcgtcg cggccgtgct ggggaggcgc gggcaccacg agcgagcgct gcgtctcgcg
     1201 ggggcgtctc ggcggatcaa ggacgatgcc ggtggcgagg cgccgcgcgc cttgatcgag
     1261 gtggaggacg cgcggaccct ggcggcgggc gctctgtccg aggagcagat ccgagcgctg
     1321 ctcgaagagg gcggacagat cggagccgaa gacgcggccg agctcgcgcg ccgcgagctc
     1381 gaggcgctcg cgccgggatg acggcacggc tcgcgcgggc actccgggtg cgcgccggcg
     1441 agggcccggt ggtcctgccc ctcttggcgc tcatgctggt cgcctgggcc gggttcgcga
     1501 tcggtgcgaa cggcgtcgag tcgatcttct tcacgcggtt cggcccgcag ttcctaccgt
     1561 ggatgtacat cgcgctcgcg ctcgtcacgg tcccggcgac gctcggcgtc ggtgcagtgc
     1621 tcgggcgcgg cgaccgccgc cgcgtggtcg cggcgttcgc gccggcgatg gcagtcgcag
     1681 tgttcgcgct tcggggcctc gcggtcgcgt cgtcggcgtg ggtgtacccg acgctgtggc
     1741 tcgggatgat ggtcctctgg atcgtgcagg ggatcgcggt ctggagcgtc gccggagtgg
     1801 tccacgacgc gcggcaggcc aagcgcctgt tcccgctcta cggggccggc gtcatcctcg
     1861 gcggtgcgct cggcggtctc gcgacgcccc cgctcgccgc gacgatcggc gcggagaacc
     1921 tgttgctggt ctggggagca tcgctggtcg ccgcgtggcc gctgtgccgg gtcgtcctcg
     1981 ccgcgccggc gcgccaccgc catccggccc tccgagggca ccgacctcgt ccgccgcggg
     2041 tcctcgagga gatgcgcgag ggattccgga ccgcacggtc ttcgccgctg ctgcggcgga
     2101 tgtcgctcgc cgccgtcctc gtcggtttcc tgtatttcac gctgacgctt ccgttcgccg
     2161 aagcggtgac cgctcggtac ccggacaccg accgcctcgc gtcgttcctc gggcttttct
     2221 cggggaccgc gaacgcgctc gcgctcgtcg tctcgctcgc ggcggccaac cgcctcttcg
     2281 ctcggttcgg cgtgcccgcg atggtgatcg tgatgccggt cgtctatctc ctcgggttcg
     2341 gcgtcgtggc ggtctcggcg tcgttcgcga cgctgatcgc gttccggctc gtgcagctcg
     2401 tctgggtcca cggcgtctac acgaccgggt ggcaggcgct cttcggcatc gtcccgccgg
     2461 agcgccgggc gcaggcgcgg acgttcgtcg agggcggtcc gatgcagctc ggcatcctta
     2521 tctcgggtgc ggtcctgctg ctcgcgcaac ggctgatcgc ggaccggacg ctgttcgtcg
     2581 tggcggcggc cgtggcggcg gccgcggtcc tcgtgtgctg gtggctgcgg cgggcgtacg
     2641 gcga
//
LOCUS       DASZOB010000302         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_76869, whole genome shotgun sequence.
ACCESSION   DASZOB010000302 DASZOB010000000
VERSION     DASZOB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76869"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VG709_04955"
     CDS             <1..1119
                     /locus_tag="VG709_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC32459.1"
                     /translation="IMRRPLPFALGTAALLVAAALPVVTLDVGPGTNRGIPQTLESVR
                     GLDVLSRAVGEGAVAPTEVAIDTGRAGGARSPEVLGAVPRLRAHALGDPEVSAVVGAA
                     MRASYVDPRERYLHLTITGRHEYGSPEAMEFARRLRSELVPVAGFPAGVAVYAGGGPP
                     SSVDVLETAYGAFPWLVLAVLVLTYFILLRAFRSLLLPLKAIVLNVLGIGAAYGLLVG
                     VFKWGAGEAIGLQAFDQVEFWIPIFLFAMVFGLSMDYEVFLVSRMREEWDATGDNVRA
                     VARGLAKTGRIVTAAGLIMFAAFMGFVAGSIVGLQQFGFGLAAAILLDVTIVRALLVP
                     SAMKLFGRWNWWLPENVARVFRVKPSPLQRKAAPASAR"
     gene            1215..>1511
                     /locus_tag="VG709_04960"
     CDS             1215..>1511
                     /locus_tag="VG709_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWC32460.1"
                     /translation="MKGVILAGGKGTRLHPLTRITNKHLLPIYDRPMVSYAIEALVKA
                     GVSEIMLVTGGTHAGEFLRLLGSGHEHGIERLAYAYQEGEGGIAEALGLAEPFVE"
BASE COUNT          192 a          539 c          540 g          240 t
ORIGIN      
        1 atcatgcgcc gcccgcttcc gttcgcgctc gggacggcgg cgctgctcgt cgcggccgcg
       61 ctgccggtgg tcacgctgga cgtcgggccg ggaacgaaca ggggcatccc gcagacgctc
      121 gagagcgttc gcggcctcga cgtcctctcc cgcgccgtcg gcgagggcgc cgtcgcgccc
      181 accgaagtcg cgatcgacac cggccgtgcc ggaggagcgc gctcgccgga ggttctcggc
      241 gcggtgcccc ggctgcgcgc gcatgcgctc ggcgacccgg aggtgagcgc ggtcgtcggc
      301 gccgccatgc gcgcgtccta cgtcgatccg cgcgagcgct atctccacct aacgatcacc
      361 gggcggcacg agtacggctc gcccgaggcg atggagtttg cgcgccggct ccgcagcgag
      421 ctcgtgccgg ttgccggctt cccggcaggc gtggccgtct acgcgggcgg cggccccccg
      481 agctccgtcg acgtcctcga gacggcctac ggcgcgttcc cgtggctcgt cctcgccgtc
      541 ctcgtcctca cgtacttcat cctcctccgc gcgttccgct cgctcctcct gccgctcaag
      601 gcgatcgtcc tgaacgtcct cgggatcggg gccgcgtacg gcctcctcgt cggcgtcttc
      661 aagtggggcg ccggcgaggc gatcgggctc caggccttcg accaggtcga gttctggatc
      721 ccgatcttcc tcttcgccat ggtgttcggc ctctcgatgg actacgaggt cttcctcgtg
      781 agccggatgc gcgaggagtg ggacgcgacc ggcgacaacg tccgcgccgt cgcgcgcggg
      841 ctcgcgaaga ccgggcgcat cgtgacggcg gcgggcctga tcatgttcgc ggccttcatg
      901 ggcttcgtgg ccggctcgat cgtcggtctc cagcagttcg ggttcgggct cgctgctgcc
      961 atcctgctcg acgtcacgat cgtgcgcgcg ctcctcgtcc cgagcgcgat gaagctgttc
     1021 ggaaggtgga actggtggct gcccgagaac gtcgcgcgcg tcttccgggt gaagccgtca
     1081 ccgctccagc ggaaggccgc gccggcaagc gcgcggtagt cgggcgtccg ggccgggcat
     1141 ggcgaaccta cggaagaatc gcaacggcga acgggtcgga gcgagcgcgg cgcgcccact
     1201 acactggccg gcgaatgaag ggcgtcatcc tcgcgggcgg caagggaacc cgcctgcacc
     1261 cgctcacgcg gataacgaac aagcacctgc tcccgatcta cgaccgcccg atggtctcgt
     1321 acgcgatcga ggcgctggtc aaggccggcg tcagcgagat catgctcgtc accggcggga
     1381 cgcatgcggg cgagtttctg cgcctgctcg ggagcggcca cgagcatggc atcgagcggc
     1441 tcgcgtacgc ctaccaggag ggcgagggcg ggatcgccga ggcgctcggt ctcgccgagc
     1501 cgttcgtcga g
//
LOCUS       DASZOB010000303         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_97339, whole genome shotgun sequence.
ACCESSION   DASZOB010000303 DASZOB010000000
VERSION     DASZOB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97339"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VG709_04965"
     CDS             <1..443
                     /locus_tag="VG709_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32461.1"
                     /translation="SCLARGRGLIVGDAAGLIDPLSGDGMFEAFVSGSLAADSIEQLL
                     AGEEAALDPYGTRLTRHLAVHLWASWSLKAALDRFPRTAYTLATGGLVWAVLEKIIAG
                     EIMDVRAVRGVARAPLKALALLARAAGDPGAAFHPPAVRPLPGD"
     gene            520..>1655
                     /locus_tag="VG709_04970"
     CDS             520..>1655
                     /locus_tag="VG709_04970"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWC32462.1"
                     /translation="MESYVVTSLPGGGRVATEPLPSVRSVAIGMWIGAGSRDESEAQA
                     GLSHFLEHLLFKGTRSYSALEIAEVFDTLGGELNASTARDYTVVYARVLDEHLPTALD
                     VMTDMVFAPVFADMDAEREVVLEEIAMIEDTPQDLVHDLFTEAVFGGHPLGRPVLGSA
                     EVISTVSRRAVTAYHRARYRLDNIVVAATGSVEHDRLVELVQETAEKRTQPPRRRPPA
                     RPPFVAPPPPSLRFERKDTEQYHVCLGAPGIARSDRRRFTASVLDAILGGSASSRLFQ
                     EVREKRGLAYAVYSFMSQYADTGQVGVYVGTREDNLAECMEIVAAELAAVAGGAVRED
                     EVERAKENLKGRIMLAMELTSNRMSRLGKSLVTESDLLSLDQVLA"
BASE COUNT          235 a          594 c          584 g          242 t
ORIGIN      
        1 attcgtgcct cgcgcgcggt cgcgggttga tcgtcggaga cgcggccggc ctgatcgacc
       61 cgctctcggg cgacggaatg ttcgaggcct tcgtgagcgg cagcctcgcc gcggattcga
      121 tcgagcagct cctcgccggc gaggaagcgg cgctcgaccc gtacggaacg cggctcaccc
      181 gccacctcgc cgtgcacctg tgggcgtcat ggagcctcaa ggcagcgctc gaccgctttc
      241 cccggaccgc atacacgctt gcgacgggcg ggctcgtctg ggcggtgctc gagaagatca
      301 tcgccgggga gatcatggac gtccgcgccg ttcgcggcgt cgcgcgcgcc ccgctcaagg
      361 cgctcgcgct gctcgcgcga gcggcgggcg atccgggcgc ggccttccac ccgccggcag
      421 tgcgccccct cccgggcgac tgagccccga agggctccgg caccggcgga ggaggaacgg
      481 cgcttccgcg ctcgcccggg ggcggactac gatgcgccgg tggagagcta cgtcgtcacc
      541 tcgctgccag gcggcggccg ggtcgcgacc gagccgcttc cgagcgtacg ttccgtcgca
      601 atcgggatgt ggatcggcgc gggctcgcgg gacgagagcg aggcgcaggc agggctctcg
      661 cacttcctcg agcatctgct cttcaagggc acgcgctcgt actcggcgct cgagatcgcc
      721 gaggtgttcg acacgctcgg cggcgagctc aacgcctcga ccgcgcgcga ctacaccgtc
      781 gtctacgcgc gcgtgctcga cgagcacctc cccacagcgc tcgacgtgat gacggacatg
      841 gtcttcgccc cggtgttcgc cgacatggac gccgagcgcg aggtcgtcct cgaggagatc
      901 gcgatgatcg aggacacgcc gcaggatctc gtccacgacc tcttcaccga ggcggtcttc
      961 ggcggtcatc cgctgggccg acccgtcctc ggctcggcgg aagtcatctc gacggtgtcg
     1021 cgccgcgccg tgaccgcgta ccaccgcgct cgctaccgcc tcgacaacat cgtcgtcgcg
     1081 gcgacgggga gcgtcgagca cgaccgtctc gtcgagctcg tgcaggagac ggccgagaag
     1141 cgcacgcagc cgccgaggcg caggccgccc gcacggccac cgttcgtcgc gccgccgccg
     1201 ccgtcgctcc ggttcgagcg gaaggacacc gagcagtacc acgtctgtct cggcgcgccc
     1261 ggcatcgccc gctccgaccg gcgtcgcttc accgcttccg tcctcgacgc gatcctcggc
     1321 ggctcggcct catcccgcct cttccaggag gttcgcgaga agcgagggct cgcctacgcc
     1381 gtgtactcgt tcatgtcgca gtacgcggac acggggcagg tcggcgtata cgtcggcacg
     1441 cgcgaagaca acctcgccga gtgcatggag atcgtcgcgg cggagctcgc ggccgtcgcg
     1501 ggcggggcgg tgcgcgagga cgaggtcgag cgggcgaagg agaacctgaa ggggaggatc
     1561 atgctcgcga tggagttgac gtcgaaccgg atgagccgcc tcgggaagtc cctcgtgacc
     1621 gagagcgacc tcctgtcgct cgaccaggtc cttgc
//
LOCUS       DASZOB010000304         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_167795, whole genome shotgun sequence.
ACCESSION   DASZOB010000304 DASZOB010000000
VERSION     DASZOB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167795"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VG709_04975"
     CDS             complement(<1..549)
                     /locus_tag="VG709_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC32463.1"
                     /translation="MRIHRLVPAALASFVVLVPLVVGPATAGAKKAPGAARAVPKSNK
                     VILFASDGMRQDTVNRYARQGAMPTFRALMRNGTQGQNGLLQGFAPNTGVGWYTLATG
                     TWPGEHGSTNNTFHRTGDQFSNRTSFATNGILQADSIQQAAERAGKTVVSVEWVGSRT
                     LDPPMQGPVVDFRSFFSDRGVLV"
     gene            715..>1972
                     /locus_tag="VG709_04980"
     CDS             715..>1972
                     /locus_tag="VG709_04980"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWC32464.1"
                     /translation="MRRLGGLLASAVTAVVLAGTAAAQVPAPPKQAVAVGRGGAAATV
                     DPLGTQAAINVLRRGGNAVDAAVAAAATLGVTEPYSCGIGGGGFMVVYRAADRRLFTV
                     DAREESPAAFQPTSFIDPATGNPIPFAEGQTSGLGVGVPGTVRSWDQALRRFGTQSLG
                     SLLQHGIRVADQGFVVDQTFHDQTEANRDRFDDFTTTRDVFLTPGTYQSPPVGSVFRN
                     PEMAATYRQIARRGPDAFYSGDIAQAIVQSVQSPPVVPGTTRNVRPGLMTLADLARYR
                     APFRDPAASGYRGYRHVGMGPPSRPHSTVGEALNILEGYPLSTMPRPQGLHYFLEASA
                     FSFADRGQYVGDPDHVNVPLAGLLSDSFAAERRALIGPTASTKPVPPGDPTDNEGPST
                     THLTVADKWGNVVSYTFTIESTGGSGI"
BASE COUNT          270 a          729 c          687 g          286 t
ORIGIN      
        1 gacgaggacg ccgcggtcgg agaagaagct ccggaagtcg acgaccggcc cctgcatggg
       61 tgggtcgagt gtccgtgagc cgacccactc gaccgacacg accgtcttgc cggcgcgctc
      121 cgccgcctgc tggatcgagt cggcctggag gatgccgttt gtcgcgaagc tcgtccggtt
      181 cgagaactgg tcgccggtcc ggtggaacgt gttgttcgtc gagccgtgct cgccgggcca
      241 cgtgcccgtc gcgagcgtgt accacccgac gcccgtgttc ggcgcgaacc cctggaggag
      301 gccgttctgg ccctgggtgc cgttgcgcat gagcgcgcgg aacgtcggca tcgcgccctg
      361 cctggcgtag cggttgaccg tgtcctggcg catcccgtcg gacgcgaaca ggatcacctt
      421 gttgctcttg gggacggcgc gggccgcccc gggtgccttc ttggcgcccg ccgtggcggg
      481 gccgacgacg agcggcacga gaacgacgaa cgacgccaga gcggcgggaa ctaggcggtg
      541 aatgcgcatg tgtcgggctc ctcccgggac cgtggggctc ggaagctaca acgctcgccg
      601 gtcgcggtca atggagcggg agacggtccg gcatcgacgc gcgtcgcgtt cacgtccccg
      661 gcacgaagtc gcctcttgac catcgtcgtc cacgggcgta acgtccgccg tccgatgcgt
      721 cgtctggggg gacttctcgc ttcggccgtc acggcggtcg tcctcgcagg caccgccgcc
      781 gcgcaggtgc ccgcaccgcc gaagcaggcg gtcgcggtcg gtcgcggcgg cgcggcggcg
      841 acggtcgatc cgctcgggac acaggctgcg atcaacgtgt tgcggcgcgg cgggaacgcg
      901 gtcgacgcgg cggtcgccgc cgcggcgacg ctcggcgtca cggagccgta ctcgtgcggc
      961 atcggcggcg gcggcttcat ggtcgtctac cgcgccgccg accggcgcct cttcacggtc
     1021 gacgctcgcg aggagtcgcc ggccgccttc cagccgacga gcttcatcga cccggcgacc
     1081 gggaacccga tccccttcgc ggaggggcag acgagcgggc tcggcgtcgg cgtccccggt
     1141 acggtgcggt cgtgggacca ggcactgcgc aggttcggca cgcaatcgct cggcagcctg
     1201 ctgcaacacg ggatccgcgt cgccgaccag ggattcgtgg tcgaccagac cttccacgac
     1261 cagaccgagg cgaaccgcga ccgcttcgac gacttcacga cgacgcgaga cgtcttcctg
     1321 acgcccggga cgtaccagtc gccgcccgtc ggctccgtct tccgcaaccc cgagatggcg
     1381 gcgacgtacc gccagatcgc acgccgcggc cccgacgcct tctactccgg agacatcgcg
     1441 caggcgatcg tgcagtcggt gcagagcccg cccgtcgttc ccgggacgac gcggaacgtc
     1501 cgcccgggac tcatgacgct cgccgacctc gctcgctatc gcgcgccgtt ccgcgacccc
     1561 gccgcgtcgg gctaccgcgg gtaccgccac gtcggcatgg gcccgccgtc gaggccccac
     1621 tcgacggtcg gcgaggcgct gaacatcctc gagggctacc cgctctcgac catgccgcgc
     1681 ccgcagggcc tccactactt cctcgaggcg tcggcgttct cgttcgccga ccgcggtcag
     1741 tacgtcggcg accccgacca cgtgaacgtc cctctcgccg gtctcctctc cgacagcttc
     1801 gcggccgagc gccgcgcgct catcgggccg acggcgtcga cgaagcccgt gccgcccggc
     1861 gatccgaccg acaacgaggg cccctcgacg acgcacctca ccgtcgcgga caagtggggc
     1921 aacgtcgtct cgtacacctt cacgatcgag tcgaccggcg gaagcggcat cg
//
LOCUS       DASZOB010000305         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_67404, whole genome shotgun sequence.
ACCESSION   DASZOB010000305 DASZOB010000000
VERSION     DASZOB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67404"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..>2482
                     /gene="secA"
                     /locus_tag="VG709_04985"
     CDS             2..>2482
                     /gene="secA"
                     /locus_tag="VG709_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWC32465.1"
                     /translation="MGAGRRRKRLQEYVELTNTFEAEVEDLSDEQLRAKTDEFRQRLA
                     GGETLDDILPEAFACVREAARRNLGQRHFDVQVMGAVALHQGNIAEMKTGEGKTLVAT
                     MPAYLNALTGDGVHVVTVNDYLAKRDSEWMTPVYRALGLQVGLIQSQMRPDERRPAYA
                     ADITFGTNNEFGFDYLRDNMAMRLDDMVQPGHHYAIVDEVDSILIDEARTPLIISGPA
                     QDSAKWYLTFARLVPRLRRDSDYEVDEQKRTVSITEEGVRRVEESLGVENLYEHVHTP
                     LVHHLQNALRAKELYKRDVDYIVKDGEVLIVDEFTGRVLHGRRYSEGLHQAIEAKEGV
                     RVREENQTLATITIQNYFRMYDKLAGMTGTAKTQAREFEEVYNLGVVEIPTNRPMIRI
                     DQQDEVYKTEDAKWKAVVDDLAQRHEKGQPVLVGTVSIEKSERLSGHLNRRGVPHHVL
                     NAKQHEKEAMIIAQAGRKGSVTVATNMAGRGVDIVLGGNPEYLARQEMAANGWDNDRY
                     LLFEMDEEERAQYEREYEPVYERFKKQTDAEHEEVVELGGLYVLGTERHESRRIDNQL
                     RGRSGRQGDPGESKFYLSLGDDLMRLFASDRIESIMNRFNWPEDEPITAKLVTRAIEN
                     AQKQVEAVNFERRKNVLKYDEVMNTQRRIVYADRRRILEGENLKEQALDMVTQVIAGT
                     VAQYVSAEIFPEEWDLDGLLTALKATYPTNLEKAQLEETVDPDELTQLVLDDAVRVYE
                     EREQELGEPVLRELERMVMLNVTDNKWREHLYEMDYLEEGIHLRAYGQRDPLTEYRGE
                     GHRMFEAMKGSIVEEFVRYIYRVELVRQD"
BASE COUNT          495 a          806 c          851 g          330 t
ORIGIN      
        1 catcggcgcc ggccggcgtc gcaagcgcct ccaggaatac gtcgagctca cgaacacgtt
       61 cgaagcagag gtcgaggacc tctccgacga gcagcttcgt gcgaagaccg acgagtttcg
      121 gcagcgcctc gcgggcggcg agacgctcga cgacatcctc ccggaggcgt tcgcgtgcgt
      181 ccgggaagcg gcgcggcgca acctcggtca gcgccacttc gacgtccagg tgatgggcgc
      241 cgtcgcgctt caccaaggca acatcgccga gatgaagacc ggcgagggga agacgctcgt
      301 cgcgacgatg cccgcgtacc tcaacgcgct gaccggcgac ggggtccacg tcgtgaccgt
      361 caacgactac ctcgcgaagc gcgactccga gtggatgacg ccggtgtacc gggcgctcgg
      421 gctccaggtc gggctgatcc agtcgcagat gcggccggac gagcgacgcc ccgcgtacgc
      481 cgcggacatc acgttcggca ccaacaacga gttcggcttc gactacctgc gcgacaacat
      541 ggcgatgcgt ctcgacgaca tggtccagcc cgggcaccac tacgcgatcg tggacgaggt
      601 cgactcgatc ctcatcgacg aggcgcgaac gccgctcatc atcagcgggc cggcgcagga
      661 ctcggccaag tggtacctga cgttcgcgcg tctcgtcccg cgcctgcgcc gggacagcga
      721 ctacgaggtc gacgagcaga agcgaaccgt ctcgatcacc gaggaaggcg tccgccgcgt
      781 cgaggagtcg ctcggcgtcg agaacctcta cgagcacgtc cacacgccgc tcgtccacca
      841 cctccagaac gcgctgcgcg cgaaggagct ctacaagcgc gacgtcgact acatcgtcaa
      901 ggacggcgag gtgctgatcg tggacgagtt caccggccgg gtcctccatg gccgccggta
      961 ctccgagggc ctccaccagg cgatcgaggc caaggagggg gtgcgcgtca gggaggagaa
     1021 ccagacgctg gcgacgatca cgatccagaa ctacttccgg atgtacgaca agctcgccgg
     1081 catgaccggt accgcgaaga cgcaggcccg cgagttcgag gaggtctaca acctcggcgt
     1141 cgtcgagatc ccgacgaacc gtccgatgat ccgcatcgac cagcaggacg aggtctacaa
     1201 gaccgaggac gcgaagtgga aggccgtcgt cgacgacctc gcccagcgcc acgagaaggg
     1261 ccagccggtg ctggtcggca ccgtgtcgat cgagaagtcc gagcggctct cagggcacct
     1321 caaccgccgc ggcgtcccgc atcacgtgct gaacgcgaag cagcacgaga aggaggcgat
     1381 gatcatcgcg caggcggggc gaaagggctc ggtgaccgtg gcgaccaaca tggccgggcg
     1441 aggcgtcgac atcgtcctcg gcggcaaccc cgagtacctc gcgcgccagg agatggcggc
     1501 gaacggctgg gacaatgacc gctacctcct gttcgagatg gatgaggagg agcgtgcgca
     1561 gtacgagcgc gagtacgagc cggtctacga gcgcttcaag aagcagacgg acgccgagca
     1621 cgaggaggtc gtcgagctcg gcgggctgta cgtcctcggg accgagcggc acgagtcgcg
     1681 gcggatcgac aaccagttgc gcggccggtc gggccgccag ggcgaccccg gcgagtcgaa
     1741 gttctacctc tcgctcggcg acgacctgat gcggctgttc gcgtccgacc gcatcgagtc
     1801 gatcatgaac cgcttcaact ggccggagga cgagccgatc acggccaagc tggtcacgcg
     1861 cgcgatcgag aatgcccaga agcaggtcga ggccgtcaac ttcgagcgcc gcaagaacgt
     1921 cctcaagtac gacgaggtca tgaacacgca gcgcaggatc gtgtacgcgg accgtcggcg
     1981 gatcctcgag ggtgagaacc tcaaggagca ggcgctcgac atggtgacgc aggtcatcgc
     2041 gggcacggtc gcgcagtacg tgagcgccga gatcttcccc gaagagtggg acctcgacgg
     2101 gctcctcacc gccctcaagg ccacctaccc gacgaatctg gagaaggcgc agctcgagga
     2161 gaccgtggac cccgacgagc tcactcagct ggtgctcgac gacgcggtcc gcgtgtacga
     2221 ggagcgcgag caggagctcg gcgagcccgt tctgcgagag ctcgagcgga tggtgatgct
     2281 caacgtcacc gacaacaagt ggcgcgagca cctctacgag atggactacc tcgaggaggg
     2341 gatccacctc cgcgcgtacg ggcagcgcga cccgctgacc gagtaccggg gcgaggggca
     2401 ccggatgttc gaggcgatga agggctcgat cgtcgaggag ttcgtgcggt acatctaccg
     2461 cgtggagctc gtccgtcagg ac
//
LOCUS       DASZOB010000306         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_127368, whole genome shotgun sequence.
ACCESSION   DASZOB010000306 DASZOB010000000
VERSION     DASZOB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127368"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VG709_04990"
     CDS             <1..622
                     /locus_tag="VG709_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC32466.1"
                     /translation="LLLDVYPELPASGRDARTPWPLPPKRTPTTYALEEGGERRWASE
                     VTEIMGRDGRVIGVHGRRVEGTSSRDLRPVPGSEFDHEADLVLVAIGFMHPEHEGLVT
                     ELALDLDKRGNVRAPVYSTRVDGVFACGDARIGQSLVVTAINEGRRCARVVDRYLGGT
                     GVARTVTAESMFAFEEGDPHSLRHQAETTGTVTVGDAFWSGPDDAG"
     gene            complement(634..1266)
                     /locus_tag="VG709_04995"
     CDS             complement(634..1266)
                     /locus_tag="VG709_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWC32467.1"
                     /translation="MDGVATKSRGRTGRRPGSSDTREAIAAAARRHFGELGYERTTIR
                     AIANEAGVDPALVVHFFGSKQKLFLSVMTLPFEPEEVIPEILAGRRSQAGLRLARFAV
                     GQLEDPRAQATITGVLRAAASEPEAASMVRDLVAGRVVGSIAETLGVADAPLRASLVG
                     SQIVGLVMARYVVRVEPLASLAPEALIEAIAPNLQRYLTGPLERERAGSS"
BASE COUNT          243 a          563 c          530 g          231 t
ORIGIN      
        1 gctgctgctc gacgtctatc ccgagctgcc ggcctccggg cgcgacgcac ggacgccgtg
       61 gccgctgcca cccaagcgca cccccaccac ctacgcgctg gaggagggcg gcgagcgtcg
      121 ctgggcctcg gaggtcacgg agatcatggg ccgcgacggc cgtgtgatcg gcgtgcacgg
      181 ccgccgcgtc gagggcacct cctcacgcga tctgcgcccg gtgcccggca gcgagttcga
      241 ccacgaggcg gacctcgtcc tggtggcgat cggcttcatg caccccgagc acgaggggct
      301 cgtcacggag ctcgcgctcg atctcgacaa gcgcggcaac gtgcgggcgc cggtgtactc
      361 cacccgagtc gacggggtct tcgcctgcgg cgacgcacga atcggccagt cgctcgtcgt
      421 gaccgccatc aacgagggcc gtcgatgcgc cagggtggtc gaccgctacc tcggcgggac
      481 gggagtggcc cgcacggtca cggcggagtc gatgttcgcg ttcgaggagg gcgatcccca
      541 ctcgctgcgt catcaggccg agacgaccgg cacggtgacg gtcggcgacg ccttctggtc
      601 gggccccgac gacgcaggct agcccgcgcg tgactaggag ctgcccgcgc gctcgcgctc
      661 gagcggcccg gtgagatagc gctggaggtt cggcgcgatc gcttcgatca gcgcctcggg
      721 agccagcgag gcaagcggct cgacacgcac gacgtaacgc gccatgacga ggccgacgat
      781 ctgcgagccg acgagactgg cgcgcagcgg cgcatcggcc acgccgagcg tctccgcgat
      841 cgacccgacg acgcgtccgg cgacgaggtc gcgcaccata ctcgcggcct ccggctcgga
      901 ggcggcggcg cggagtacgc cggtgatcgt ggcctgcgca cgcggatcct cgagctggcc
      961 gaccgcgaag cgcgcgagcc gcagccccgc ctgcgacctg cggccggcga gtatctccgg
     1021 tatgacctcc tccggctcga acgggagcgt catgaccgat aggaacagct tctgcttcga
     1081 accgaagaag tgcacgacca gcgccgggtc gacacccgcc tcgttcgcga tggctcggat
     1141 cgtcgttcgc tcgtagccga gttcgccaaa gtgccgccgc gccgccgcgg cgatcgcctc
     1201 gcgcgtgtcg ctcgacccgg gtcgccgacc ggtgcgccct ctcgacttcg tggccacgcc
     1261 gtccatatta agtcaacgac cgttgacagt gtaagtcaac gagcgtagaa ttcaacgtac
     1321 gttgaacgaa gtcacgtcga ggagaaagca ccatgtcgtc gctcgccgca gcccccgcag
     1381 cccccgcgga catccccgcg ccactgtcca gccgttggag ggtgctggga gtgctctcgc
     1441 tcagccttct gataatcggg ctcgacaaca cgatcctcaa cgtcgcgctt ccacgctgca
     1501 ggacgagttc ggcgcctcag cctcgacgct gcagtggatt gtcgactcct acctgctcgt
     1561 cttcgcc
//
LOCUS       DASZOB010000307         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_174758, whole genome shotgun sequence.
ACCESSION   DASZOB010000307 DASZOB010000000
VERSION     DASZOB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174758"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1776
                     /locus_tag="VG709_05000"
     CDS             94..1776
                     /locus_tag="VG709_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWC32468.1"
                     /translation="MDAQHDVLIVGAGCAGMRAAIEAHDAGADVALLSKIHPVRSHSG
                     AAEGGINAALGNASEDDPEKHAFDTVKGSDYLGDQDSIEVLCNEAPDDVYELEHWGAV
                     FSRTEDGRIAQRPFGAAGEPRTAYAEDITGHVLVHVLYEQVMKRDILTYEEWFAWKLV
                     EDDGRCQGVIAWDILNGGLKTVGAKTVILATGGAGRLYRGTTNAYACTGDGMTMALRL
                     GVPLKDMEMMQFHPTTLSPTGVLITEGCRGEGAYLLNAEGERFLKSYAPNAMELASRD
                     VISRAEQTEIDEGRGVDGNVLLDLRHLGAERILERLHGTRELSMVFAGVDPIHEPIPV
                     RPGAHYHMGGVDTDLWGKTSLEALYAAGECACVSVHGANRLGGNALMETVTFGRRAGA
                     AAAEWALTHTTVAVPESAVSDAERELRVLLDREQGERPHAIRDEMAETMHENFGVFRR
                     EDQMRAQGGIVARLQERFERVVVDDKGSVFNNDVTQALELGFLLDLAACMVAAGLARK
                     ESRGAHARPHDYPERDDENYMRHTLVRWGDGAPERDWAPGRVTKWEPQERTY"
     gene            1782..>2061
                     /locus_tag="VG709_05005"
     CDS             1782..>2061
                     /locus_tag="VG709_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32469.1"
                     /translation="MTGMRTAWSAIAVAAALAVGAVAGGCGGGDDVSPQAAVADAATK
                     TADAGTSRLALEVETELPGEEPVAFTGTGVFDYDAQRGRMAMDMSELLE"
BASE COUNT          317 a          698 c          781 g          265 t
ORIGIN      
        1 tgccggagcg ccgcgacgtc gtggtgccgc gccgcgcctc ccgggggagg atgcggggct
       61 cggcgcgggt gccgtcgtat agcgtttggc tcggtggacg cccagcacga cgtgctcatc
      121 gtcggtgccg gctgtgccgg catgcgcgcc gcgatcgagg cccacgacgc cggggccgac
      181 gtcgcgcttc tctcgaagat ccaccccgtc cggagtcact cgggggcggc ggagggcggg
      241 atcaacgcag cgctcgggaa cgcgtccgag gacgatccgg agaagcacgc gttcgacacg
      301 gtcaagggat ccgactacct cggcgaccag gactcgatcg aagtgctctg caacgaggcg
      361 ccggacgacg tctacgagct cgagcactgg ggcgccgtct tctcgcgcac ggaggacggc
      421 cgcatcgcgc agcggccctt cggtgccgcc ggcgagcccc gcacggcgta cgcggaggac
      481 atcacgggcc acgtgctcgt ccacgtcctc tacgagcagg tgatgaagcg cgacatcctc
      541 acgtacgagg agtggttcgc ctggaagctg gtcgaggacg acggccgctg ccagggcgtt
      601 atcgcctggg acatcctgaa cggcggcctc aagacggtcg gcgcgaagac ggtgatcctc
      661 gccactggag gcgcgggccg cctctaccgc ggcacgacga acgcgtacgc ctgcacgggc
      721 gacgggatga cgatggcgct ccgcctcggc gtcccgctca aggacatgga gatgatgcag
      781 ttccacccga cgacgctctc gccgacgggc gtgctcatca ccgagggctg ccgcggtgag
      841 ggcgcgtacc tcctgaacgc ggagggcgag cgcttcctca agagctacgc gccgaacgcg
      901 atggagctcg cgagccgcga cgtgatctcg cgcgcggagc agacggagat cgacgagggg
      961 cgcggcgtcg acgggaacgt tctcctcgac ctgcgccacc tcggcgccga gcggatcctc
     1021 gagcgcctgc acgggacacg cgagctctcg atggtcttcg cgggcgtcga cccgatccac
     1081 gaaccgatcc ccgtgcgacc cggggcgcac tatcacatgg ggggcgtcga caccgacctc
     1141 tggggcaaga cgtcgctcga ggccctctac gcggccggcg agtgtgcgtg cgtctccgtc
     1201 cacggagcca accgcctcgg cggcaacgcg ctcatggaga cggtcacgtt cgggcgccgc
     1261 gcgggcgccg cggcggccga atgggcgctc acgcatacga cggtcgcggt gccggagagc
     1321 gcggtctccg acgccgagcg ggagctccgg gtcctcctcg accgggagca gggcgagcgg
     1381 ccgcacgcga tccgcgacga gatggcggag acgatgcacg agaacttcgg cgtcttccgg
     1441 cgcgaggatc agatgcgggc gcagggcggg atcgtcgcgc gcctgcagga gcggttcgag
     1501 cgcgtcgtcg tggacgacaa gggctccgtc ttcaacaacg acgtcacgca ggcgctcgag
     1561 ctcggttttc tgctcgacct cgccgcgtgc atggtcgctg ccgggctggc gcggaaggag
     1621 agccgcgggg ctcacgcgcg cccgcatgat tatcccgagc gcgacgacga gaactacatg
     1681 cgccacactc tcgtccgctg gggcgacggc gccccggagc gcgactgggc gcccggccgc
     1741 gtgacgaagt gggagccgca ggagcggacg tactgaggac ggtgacggga atgcgcacgg
     1801 catggagcgc gatcgcggtc gcggccgccc tggccgtcgg cgcggtcgcc ggtggctgcg
     1861 gcggcgggga cgacgtctcg ccgcaggcgg ccgtcgcgga cgcagcgacg aagacggccg
     1921 acgccggcac gagtcggctc gcgctcgagg tggagacgga gctccccggc gaagagccgg
     1981 tcgcgttcac cggcacgggc gtcttcgact acgacgcgca gcgcggccgc atggcgatgg
     2041 acatgtcgga gctgctggag g
//
LOCUS       DASZOB010000308         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_6881,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000308 DASZOB010000000
VERSION     DASZOB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6881"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VG709_05010"
     CDS             complement(<1..1089)
                     /locus_tag="VG709_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC32470.1"
                     /translation="MGISTAPPQGGRLPASLVERVGTLPPRFRREAADGFAADFALAI
                     DGHGFQVEILRGACAVRTPESRFPTASVATDVETWEALDDGALTSIEAFLAGRVAVRG
                     NVEHAVRLQSLFHPAARPRSVRDLEHLTLSADGHTISAYELGEGPTVMLLHGLGASKV
                     SWLPLLSRLAERYRVLVPDLPGHGESSKPRVNYTPPFYAAIATRILDVVGVERAALVG
                     NSMGGRDAALPDLPVPYYARFARILPTEIAAVPLPLRRRLVTNYIRQLFADPSRLPPG
                     AYLAGADEFLRIYRSGRARVALLSSLRGLIRDPERPFWSAARSIAAPTLILWGREDRL
                     VPVRFGRRLAAEMPAAEFRVFDGVGHVPQ"
     gene            1129..2523
                     /locus_tag="VG709_05015"
     CDS             1129..2523
                     /locus_tag="VG709_05015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWC32471.1"
                     /translation="MNERLSTLDASFLYLEKPNVHMHVAGLAILDPSTRPDGRLGIDD
                     ITRLIEQRIHLVPRFRQRVVFPPLSAGRPVWADDPTFDLEFHTRRAALPAPGGRRELA
                     DFVQRVHSRPLDRTKPLWEMYLIEGLEDDHVAILSKSHHAMIDGLSGMDIATVMFDFS
                     ADTAEVKAPTAWEPSPEPAPGDLWLDAIRDQISHPVRTAVENAARAIRAPQEAWHRVR
                     TTLGGVAAVLAKGQADAGPFNGRIGSNRRFAMAEVPVADMKAIKNALGGTVNDVVLAS
                     VTGGLRTLLRSRKERVTGVTLRAMVPVSTRDDSRTMALGNQVSMFFVDLPVGVRDPVK
                     RLRTISRETEQLKSSHAAVGAAAIMNTARWAPPTLHALAARLVSRSRFANLVVSNVPG
                     PQVPLYLAGAKLVIVYPVMPLAETLGLSVAVTSLSGTMGFGFTSDWGSIPDIDVMANG
                     LLDAISDLKKAARV"
     gene            complement(2520..>2852)
                     /locus_tag="VG709_05020"
     CDS             complement(2520..>2852)
                     /locus_tag="VG709_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWC32472.1"
                     /translation="PWADVSMLRAARSMLGIILRRRHFQRLIRRVEAPTLMVHGEADR
                     LVRHAAAKVVATVRPDWTFQTIADAGHTPMLEKPQETAEAIFRWLDGDGRAALGRATR
                     APADAPAG"
BASE COUNT          442 a         1001 c         1018 g          391 t
ORIGIN      
        1 ctgcggcacg tgcccgacgc catcgaagac gcggaactcc gcggcgggca tctccgcggc
       61 gaggcgacgc ccgaaccgca cggggacgag gcgatcctcc cggccccaca ggatcagcgt
      121 cggcgcggcg atgctgcgcg cggcgctcca gaagggtcgc tccggatcgc ggatcagccc
      181 ccggagcgac gacagcagcg cgacgcgcgc gcgtccggaa cgatagatcc gcaggaactc
      241 atcggcgccc gcgaggtacg cccccggcgg gagccgcgag gggtcggcga acagctggcg
      301 gatgtagttc gtgacgagcc tgcgccggag cggaagcggc acggcagcga tctccgtcgg
      361 aaggatcctc gcgaaccgcg cgtagtacgg gacggggaga tccggcagag cggcgtcgcg
      421 gccgcccatc gagttcccca cgagcgcggc gcgctccacg ccgacgacgt cgaggatcct
      481 cgtggcgatc gcggcgtaga agggcggcgt gtagttgacg cggggcttgg acgactcgcc
      541 gtgccccgga aggtcgggaa ccagtacgcg gtagcgctcg gcgagccgag agagcagcgg
      601 gagccacgac accttcgagg cgccgaggcc gtggaggagc atcacggtcg gcccctctcc
      661 gagctcgtac gccgagatcg tgtgcccgtc ggcggaaagc gtgaggtgct cgaggtcgcg
      721 gaccgatcgc ggcctcgccg ccgggtggaa gagcgactgc agccggacgg cgtgctcgac
      781 gttgccgcga accgccacgc gaccggcgag gaacgcctcg atgctcgtca gcgcgccgtc
      841 gtcgagggcc tcccaggtct cgacgtcggt cgcgacgctc gccgtcggga accgcgactc
      901 gggcgttcgg acggcgcagg cgccgcgcag gatctcgacc tggaagccgt gcccgtcgat
      961 cgcgagcgcg aagtcggcgg cgaacccgtc cgcggcctcc cgcctgaacc tcggcggcag
     1021 ggtcccgacg cgttcgacga gcgacgcggg caggcgtccc ccttgcgggg gcgccgtcga
     1081 gatccccacg gcgctaatct agtggaggaa gcgacgaaag gtgccccgat gaacgagcga
     1141 ctgtcgacgc tcgacgcgtc cttcctgtac ctggagaagc cgaacgtgca catgcacgtg
     1201 gccggcctcg cgatcctcga tccgtcgacc cgtcccgacg ggaggctcgg gatcgacgac
     1261 atcacgcgtc tgatcgagca aaggatccac ctcgtcccgc gcttccgcca acgtgtggta
     1321 ttccctccac tgtctgccgg gcgaccggtc tgggccgacg acccgacctt cgacctcgag
     1381 ttccacacgc gccgcgccgc cctccccgca ccggggggcc gacgagagct cgccgacttc
     1441 gtccagcgcg tgcactctcg tccgctcgac cggacgaagc cgctctggga gatgtacctc
     1501 atcgagggcc ttgaggacga ccacgtcgcg atcctgtcga agtcgcacca cgcgatgatc
     1561 gacgggctct cggggatgga catcgcgacc gtgatgttcg acttctccgc cgataccgcc
     1621 gaggtgaagg caccgacggc gtgggaaccg tcgcccgagc cggcgcccgg cgacctgtgg
     1681 ctggacgcta tccgcgatca gatctcgcat ccggtcagaa ccgccgtgga gaacgcggct
     1741 cgcgcgatcc gcgcgccgca ggaggcgtgg catcgcgtcc ggacgacgct cggaggcgtc
     1801 gccgccgtcc tcgcgaaggg tcaggccgac gcgggcccct tcaacggccg gatcggctcg
     1861 aaccggcggt tcgccatggc cgaggttccc gtcgccgaca tgaaggcgat caagaacgcg
     1921 ctcggcggga ccgtaaacga cgtcgttctc gcgtcggtga ccggcggcct ccgcaccctg
     1981 ctgagatctc ggaaggaacg cgtaaccggc gtgacgctgc gtgcgatggt tccggtctcg
     2041 acacgcgacg acagccggac gatggcgctc gggaaccagg tgtcgatgtt cttcgtggat
     2101 ctcccggtcg gcgtccgcga ccccgtgaag cgtctccgca cgatctcgcg cgagaccgag
     2161 cagctgaaat cgtcgcacgc cgcggtcggc gccgcggcga tcatgaacac ggcgaggtgg
     2221 gcgccgccga cgctccacgc cctcgccgcg cggctggtgt cgcgctcgcg attcgcgaac
     2281 ctcgtcgtgt cgaacgtccc ggggccgcag gtgcctctgt atctggctgg cgcgaagctc
     2341 gtgatcgtct accccgtgat gccgctcgcc gaaacgctgg ggctctcggt cgcggtcacg
     2401 agcctctcgg gcacgatggg gttcgggttc accagcgact ggggctcgat ccccgacatc
     2461 gacgtgatgg cgaacggtct cctcgatgcc atctcggacc tcaagaaggc ggcccgcgtc
     2521 taaccggcgg gagcgtcggc cggcgcgcga gtcgcgcgac ccagggccgc ccggccgtca
     2581 ccgtcgagcc agcggaagat cgcctcggcc gtctcctgcg gcttttcgag catcggcgtg
     2641 tgacccgcgt cggcgatcgt ctggaacgtc cagtccggcc ggacggtcgc gacgaccttg
     2701 gccgcggcgt gacggaccag gcgatcggct tcgccgtgga ccatgagcgt cggcgcctcg
     2761 acccgccgga tcagacgttg gaagtggcgg cgccgcagga tgatcccaag catcgaacgg
     2821 gcggcgcgca gcatcgagac gtcggcccag gg
//
LOCUS       DASZOB010000309         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_147723, whole genome shotgun sequence.
ACCESSION   DASZOB010000309 DASZOB010000000
VERSION     DASZOB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147723"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1221)
                     /locus_tag="VG709_05025"
     CDS             complement(121..1221)
                     /locus_tag="VG709_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HWC32473.1"
                     /translation="MPPTVRDWTSLLDDAFPPSWAEPWDNPGLQVGGADWPADRVVVA
                     LDPAPEVVAEGRELGAGLIVTHHPLVFRPLARLDEGDATARIALEAARAGIAVFACHT
                     NADVAGGGVSDALAEALDLEVTGVLEATAAGERVKLVTFVPPEATAKVLDAVAGAGGG
                     VIGEYTHCSFRVRGSGTFLPSERAQPVVGERGELNEAEEDRLEIVVPRERLSTATRAM
                     IEAHPYEEVAYDAYQLVGGGLGLGRVTRLREPVTGDELAGRCAERLGASVRVAGPRDR
                     PVERVAVCGGSGGSLVAAAIRCGADAYVTGDVKHHEALDARAAGLTVIDAGHHATEWP
                     FVPRLAALLSDRGEGLGSVEISNVSTDPFAQR"
     gene            complement(1375..>1705)
                     /locus_tag="VG709_05030"
     CDS             complement(1375..>1705)
                     /locus_tag="VG709_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWC32474.1"
                     /translation="FTSKDFRTWAGTVLAATALRLAGGFRSEREAKRKVTRAIEAVAE
                     ELGNTPAVCRASYVHPAIIEAYVADELGSPNGSPKRRTRYGLRIDERWVLDMLRRRLR
                     RTRRKVA"
BASE COUNT          234 a          647 c          603 g          221 t
ORIGIN      
        1 cctcgacgcg cttgcggcac gccgcgacct cgtcgccacg gaggagctcc tgccgccgcg
       61 cctcgaggcg gtcgatcgac aggtccagct cctggagctc gaggatgcgg tcgagcccct
      121 tcatcgctgc gcgaagggat ccgtcgaaac gttcgagatc tcgacgcttc cgaggccttc
      181 gccgcggtcc gacagcagcg ccgcgagccg ggggacgaac ggccattccg tcgcgtggtg
      241 accggcatcg atcaccgtca gaccggcggc gcgggcgtcg agcgcctcgt ggtgcttcac
      301 atctcccgtg acgtacgcgt cggcgccgca ccggatcgcg gcggcgacga gcgacccgcc
      361 ggatccgccg cacaccgcca cgcgctcaac cggtcgatcc cgcggccccg cgacacggac
      421 cgaggcaccg aggcgctccg cgcagcgacc ggccagctcg tcgccggtga ccggctcacg
      481 gaggcgcgtg acgcggccga gcccgagccc tccgcccacg agctggtagg cgtcgtacgc
      541 gacctcctcg tacgggtgcg cttcgatcat ggcccgggtg gcggtggaaa gccgctcgcg
      601 cggcacgacg atctcgagcc tgtcctcctc cgcttcgttc agctcgccgc gctcccccac
      661 gaccggttgt gcccgctccg acggcaggaa cgtcccggag ccacggacgc ggaacgagca
      721 atgcgtgtat tcgccgatca cgccgccacc ggcgccggcc accgcgtcga ggaccttcgc
      781 ggtcgcctcc ggaggcacga acgtgacgag cttcacgcgc tccccggctg cggtcgcctc
      841 gagcacgccc gtcacctcga ggtcgagcgc ctcggccagc gcgtcggaga cgccgccgcc
      901 cgcgacgtcg gcgttcgtgt ggcacgcgaa cacggcgatg cccgcgcgcg ccgcttccag
      961 cgcgatgcga gccgtcgcgt ctccctcgtc gagacgcgcg aggggacgga acacgagcgg
     1021 gtgatgggtc acgatcagcc ccgcgccgag ctcgcggccc tcggcgacga cctcgggggc
     1081 cgggtcgagg gcgacgacga cccggtcggc gggccagtcg gcgccgccca cctggagccc
     1141 cgggttgtcc cacggctccg cccacgacgg cgggaaagcg tcgtcgagga gcgacgtcca
     1201 gtcgcggacg gtcggaggca tcaggacgga caggatagcg ccgggcgacg gcgtctcagc
     1261 gtagcggcag ccgcaccccg acggggcctg cgacggcgac cgcggaagct gatctcgcgt
     1321 gacgcatcat gcgcgcggcg ctacttgggt acggcgcgtc gcattccttc ggccctacgc
     1381 gaccttccgt cgcgtccggc ggaggcggcg acgcagcatg tcgagcaccc accgctcgtc
     1441 gatccggagc ccgtagcgag tccggcgctt cggggagccg ttcggggagc cgagctcatc
     1501 ggcgacgtac gcctcgatga tcgccggatg gacgtagctg gcccggcaga cggccggcgt
     1561 gttcccgagc tcctccgcga cggcctcgat cgcgcgcgtc acctttcgct tcgcctcgcg
     1621 ctcggaccgg aagccgccgg cgaggcggag cgccgtcgcg gcgagcacgg ttccggccca
     1681 cgtccggaaa tccttgctcg tgaac
//
LOCUS       DASZOB010000310         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116739, whole genome shotgun sequence.
ACCESSION   DASZOB010000310 DASZOB010000000
VERSION     DASZOB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116739"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VG709_05035"
     CDS             complement(<1..206)
                     /locus_tag="VG709_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWC32475.1"
                     /translation="MRVALAQPNLTVGDIDANVERLAAWSREATGHGADVVCFPELAI
                     TGYPPEDLVLRREFVDDNLQALEDL"
     gene            complement(234..>2440)
                     /locus_tag="VG709_05040"
     CDS             complement(234..>2440)
                     /locus_tag="VG709_05040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32476.1"
                     /translation="EDRDLIAARRGHLIGLTNAPAELHEMWWGVRRFLEALAQRRPVI
                     AIFDDLHWAQPALLDLVEYLAESTRHAPILLLCLARPELLDDRPKWGGGKVNATSILL
                     EPLSGETAGSLVENLLGTADVALGVKSRIADAADGNPLFVEEILAMFIDEGRLLREDG
                     RWIPTTDLSDVAVPPTIQALLAARLERLPADERAVLERASVEGKVFHVGAVAELVPAR
                     IGDRVGEHLRTLVRKELIRPERESFMGQDAFSFRHILLRDEAYRGMPKETRAALHERF
                     AAWLERSAGEWLPEYREIVGYHLEQSYRAHADLGPIGRATAETGRRAAEHLDVAGRRA
                     LDRGDVRAAANLFDRALAMLPDVDAGGVRLRIRLAEALTEMGEFARADDVLERALDES
                     RDVGDERLTWLALMQRATLRINTDPGNSTAEARRDAQDAIRYFTQLGDDEGLARAWYV
                     LAFVAWNEARTDATTEALEHVIRHADATGDVRLKTAALALMAAAASWGALPVDEGIRR
                     ADDVLRRGKGHSLVESVALAAQGTLAAHRGRFDDARALIARSREILAELGQTVMHAAT
                     AQDSGRVEMLAGDAVAAEREFRGGYELLERIGERAYLSTIAALLAEALHEQGRLDEAL
                     RFTQVSEEAADPRDLASQISWRMTRAKVLAGRGDHGAAERLARDAVALAAGTDILDTH
                     GDALLSLGEVLREAGRAEEARAAFHDALELYERKGASVRADAAQAILAGLGG"
BASE COUNT          317 a          898 c          879 g          346 t
ORIGIN      
        1 aggtcctcca gcgcctgcag gttgtcgtcg acgaactcgc ggcgcaggac caggtcctcc
       61 ggcgggtagc ccgttatcgc gagctccggg aagcagacga cgtcggcccc gtgccccgtc
      121 gcctcgcggc tccatgcggc gagcctctcg acgttggcgt cgatgtcgcc gaccgtgagg
      181 ttgggctggg ccagtgcgac cctcacggcg aaagcgtagc gcgagggcgg ccgtcagccg
      241 ccgagcccgg cgaggatcgc ctgcgccgcg tcggcgcgca ccgaggcgcc cttgcgctcg
      301 tacagctcga gcgcgtcgtg gaacgcggca cgtgcctcct cggctcgccc cgcctcccgt
      361 agcacctccc cgaggctgag aagcgcatcg ccgtgcgtgt cgaggatgtc cgtccccgct
      421 gcgagagcga ccgcgtcgcg cgcgaggcgc tctgccgccc cgtgatcgcc gcggcccgcg
      481 agcaccttgg cgcgcgtcat ccgccaggag atctgcgacg cgaggtcgcg cgggtcggcg
      541 gcttcctcgc tgacctgggt gaaacgcagc gcctcgtcga gacgcccctg ctcgtggagc
      601 gcctcggcga ggagtgccgc gatcgtcgag aggtacgcgc gttcgccgat gcgctcgagg
      661 agctcgtagc cgccgcggaa ctcccgttcg gccgcgaccg cgtcgccggc gagcatctcg
      721 acccgcccgc tgtcctgcgc ggtcgccgcg tgcatcaccg tctggccgag ctcggcgagg
      781 atctctcgcg agcgggcgat cagcgcccgt gcgtcgtcga agcgcccgcg atgcgcggcg
      841 agcgtgccct gagcggcgag cgcgaccgac tccacgaggc tatgtccctt tcctcgccgc
      901 agcacgtcgt cggcgcgacg gatcccttcg tcgaccggca gcgcgcccca gctcgcggcg
      961 gccgccatga gcgcgagcgc cgcggtcttc aagcggacgt cgcccgtcgc gtcggcgtgc
     1021 cggatcacgt gctcgagtgc ttcggtcgtg gcgtccgtcc gagcctcgtt ccacgccacg
     1081 aaggcgagga cgtaccacgc gcgcgccagc ccctcgtcgt caccgagctg ggtgaaatac
     1141 cggatcgcgt cctgcgcgtc gcggcgggcc tccgccgtgc tgttcccggg gtcggtgttg
     1201 atccggagcg tggcgcgctg catcagcgcg agccacgtca ggcgctcgtc accgacgtcc
     1261 cgcgactcgt cgagcgcgcg ctcgagcacg tcgtccgcgc gcgcgaactc gcccatctcg
     1321 gtgagggcct cggcgaggcg gatccggaga cgcacgccgc cggcgtcgac gtcggggagc
     1381 atcgcgaggg cacggtcgaa taggttcgcg gccgcgcgca cgtcgccgcg atcgagcgcg
     1441 cgtcgcccgg cgacgtcgag gtgctccgcc gcacgccggc cggtctccgc ggtcgcgcga
     1501 ccgatcggcc cgaggtcggc atgcgcgcgg tacgactgct cgaggtgata cccgacgatc
     1561 tctcggtact ccggcagcca ctcgcccgcg gagcgctcga gccacgcggc gaaccgctcg
     1621 tggagggcgg cgcgcgtctc cttcggcatg ccgcggtacg cctcgtcccg caggaggatg
     1681 tggcggaacg agaacgcgtc ctgtcccatg aacgactcgc gctcgggccg gatgagctcc
     1741 ttgcggacca gcgtgcgcag gtgttcgccg acgcggtcgc cgatccgcgc ggggacgagc
     1801 tcggcgacgg cgcccacgtg gaacaccttg ccctccacgg atgctcgctc cagcaccgct
     1861 cgctcgtcgg cgggcaggcg ctcgagacgc gccgccagca gcgcctggat cgtcggcgga
     1921 acggcgacgt cggacagatc cgtggtgggg atccaccggc cgtcctcgcg gaggagcctc
     1981 ccctcgtcga tgaacatcgc gaggatctcc tcgacgaaca gggggttccc atcggccgcg
     2041 tcggcgatcc gcgacttgac gccgagagcg acgtcggccg ttccgagaag gttctcgacg
     2101 agcgacccag ccgtctcgcc ggacagcggt tcgagaagga tcgacgtcgc gttgaccttt
     2161 ccgcctcccc acttcggtcg gtcgtccagg agctcgggcc gcgcgaggca cagcaggagg
     2221 atcggcgcgt gacgcgtcga ctccgcgagg tactcgacga ggtcgaggag cgccggctga
     2281 gcccagtgga ggtcgtcgaa gatcgcgatc acgggccgtc gctgcgcgag cgcctcgagg
     2341 aagcgacgga ccccccacca catctcgtgg agctcggccg gcgcgttcgt gagcccgatc
     2401 aggtggccgc ggcgcgccgc gatcaggtcg cggtcctccg
//
LOCUS       DASZOB010000311         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_107897, whole genome shotgun sequence.
ACCESSION   DASZOB010000311 DASZOB010000000
VERSION     DASZOB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107897"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VG709_05045"
     CDS             complement(<1..1720)
                     /locus_tag="VG709_05045"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWC32477.1"
                     /translation="MAEPSGSDRRLLIASLLFLALATAAGFGRVFAGGGATVRLAVAA
                     AVSVLVATVFVRRNLVLGLAGSAVALLVVLGILVYRDTLWFGVPGPGTLEGIARSLEH
                     LGRDAAREIAPAPALSSLMTAALIAVWAAATAAHALAVRARSPILALLPGAVLIAFAG
                     VVTAEPPRPLFAIPYLVAAIGVLYGASLERLHTWGPLLERRGRRALAGGSTGRWARRL
                     GVAAAAVAVAMPGVLPGLGASPLMTVDPGRDGGTVSINAIVDMRPALLQQPVSELFVV
                     DAPRPAYWRLLSLDRFDGRTWTSTDSRARHGRILDGAQLPGESDARGWGLVQTFEIRS
                     LSVPWLPAAYRPVEIDGDADADRWDPDAGVLATNTGTRTGDTYTVRSKLFQPKPEALD
                     RAYAGGRPRGLERYVEVPALPQELESVLGRITRGHETPYRQAVAIQDYLRSFRYDLSA
                     PVGHDTSDIVFFLTESQRGYCEQFAGSMAVLLRMLGIPARVAVGFAPGTPTGDGRWLV
                     TTQDAHAWVEAYFPDHGWIAFEPTPTRSIPLARPYVLPVPAIAMHSLTGLPAQPRFRP
                     APRSAAE"
     gene            complement(1681..2913)
                     /locus_tag="VG709_05050"
     CDS             complement(1681..2913)
                     /locus_tag="VG709_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWC32478.1"
                     /translation="MLTARAAAVLTGATLLWLGSRVVGSAEIHIVAVGLLALLVLAVL
                     VMRSLPRDLRATRRISTPRAFPGARVRVDVDVENRGGLRTSLVLLEDRLPASLGPPAR
                     AVLPSVPAQGSERFSYHVTCRTRGRHVIGPLSASISDPFDLVRRQLRFTERHELVVYP
                     EVEDLDRALVPAPPGGRGDSRSRQLFRTGEDFYTMRAYEVGDDLRRIHWPSVARTGEL
                     MIRQEQAARPAVIALFVDTRRRGLAATPEAFERGVSAAASVGELYVRSGYGVRLATSE
                     TPPRTLARPELLEALAVLDPTRGHALSPGLERLRVFASGGSTLVVVTHVPDAAETATL
                     VRSGAGFPKRIAVLVHPRDRAALSPNERSATAERAEGVRISLARSGWDVVTVEPSERL
                     AGRWTTWRSRAARTGASS"
     gene            complement(2917..3876)
                     /locus_tag="VG709_05055"
     CDS             complement(2917..3876)
                     /locus_tag="VG709_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWC32479.1"
                     /translation="MQKPNAWGWFQALFDRIHTNVERAIQGKPREIRLALVTLVAEGH
                     LLIEDVPGVGKTMLAKAMARSIDCSFRRIQFTPDLLPTDINGVNVFNQERGDFEFKPG
                     AIFANVVLGDEINRASPKTQSALLESMEERQVTVDGISYQLGTPFMVIATQNPIEHEG
                     TYPLPESQLDRFMMRLEVGYPGADAEMDILSTHGTGSATFDVEPVTDAASVGRLIALA
                     REVHVADAIRRYIVGIAEATRRHPDIYLGASPRAAIMLLRASRAFAASDGRDYVVPDD
                     VKGIAVPILAHRLIVSADAGLSGRDARVVLGSVLDDVPVPVKG"
BASE COUNT          603 a         1417 c         1460 g          526 t
ORIGIN      
        1 cttcggcagc cgacctcgga gccgggcgga agcgcggctg cgccgggagc ccggtaagcg
       61 agtgcatcgc gatggcgggg accggcagca cgtagggccg cgcgagcggg atcgaccgcg
      121 tcggcgtcgg ctcgaacgcg atccacccat ggtccgggaa gtacgcctcg acccacgcgt
      181 gggcgtcctg cgtcgtgacg agccagcggc cgtcgcccgt cggcgtcccc ggcgcgaacc
      241 caaccgcgac gcgagccggg atcccgagca tccgaagcag cacggccatc gatccagcga
      301 actgctcgca gtagccgcgc tgtgactcgg tgaggaagaa gacgatgtcg gaagtgtcgt
      361 gtccgaccgg cgccgagagg tcgtagcgga acgacctcag gtagtcctgg atagcgacgg
      421 cctggcggta cggtgtctcg tggccgcgcg tgatgcgacc aaggaccgat tcgagctcct
      481 gcgggagcgc gggaacctcg acgtatcgct cgagccctct gggccgtccc cccgcgtagg
      541 cgcgatcgag cgcctcgggt ttcggctgga agagcttcga tcgtacggtg tacgtgtcgc
      601 cggtacgcgt cccggtgttc gtggcaagga ccccggcgtc ggggtcccag cgatccgcat
      661 cggcgtcgcc gtcgatctcg accggtcggt atgcggcggg gagccacggc acggagagcg
      721 agcggatctc gaaggtctgg acgagtcccc agccgcgggc gtccgactcg cccgggagtt
      781 gcgccccgtc gaggatcctg ccgtggcgcg cacgcgagtc cgtgctggtc caggtgcggc
      841 cgtcgaaccg gtcgagcgag agcagccgcc agtaggcggg ccgcggcgcg tcgacgacga
      901 agagctccga aaccggctgc tgcaggagcg cgggccgcat gtcgacgatc gcgttgatcg
      961 agaccgtccc gccgtctctt cccgggtcga cggtcatgag cggcgacgcg ccgagacccg
     1021 gaaggacgcc gggcatggcg accgcgacgg cggcggccgc gactccgagc cgcctcgccc
     1081 accgcccggt gctccctccg gcaagggcgc gccgtccgcg gcgctcgagc agtggccccc
     1141 acgtatggag gcgttcgagg ctcgcgccgt acagcacgcc gatcgccgcg acgaggtagg
     1201 ggatcgcgaa cagcggtcga ggtggctccg ccgtgacgac gccggcgaac gcgatgagaa
     1261 ccgcccccgg cagcagcgca aggatggggc tgcgggcccg gacggcgagc gcatgcgcgg
     1321 cggtcgccgc cgcccacacc gctatcagcg cggctgtcat cagcgacgag agcgccggcg
     1381 ccggcgcgat ctccctcgcg gcgtcgcgtc cgaggtgctc gagcgagcgc gcgatcccct
     1441 cgagcgtccc cggtcccgga acgccgaacc agagcgtgtc ccgatacacc aagatcccga
     1501 gcaccacgag gagcgcgacg gcggagccgg ccagccccag cacgaggttc cgccggacga
     1561 agaccgtcgc caccagcacc gacacggcgg ccgcgacggc gagccgtacc gtcgcaccgc
     1621 cccccgcgaa cacgcgcccg aatccggccg ccgtcgcgag cgcgaggaac agcagagagg
     1681 ctatgaggag gcgccggtcc gagccgctcg gctccgccac gtcgtccacc tccccgccag
     1741 acgctcgctc ggctcgaccg tcacgacgtc ccatcccgac ctcgcgagcg agatccggac
     1801 accttccgcg cgctcggccg tcgcggaccg ctcgttcggc gacaacgcgg cgcggtcacg
     1861 cggatggacg agcaccgcga tcctcttcgg gaatcccgca cccgaccgca ccagcgttgc
     1921 cgtctcggcg gcgtcgggga cgtgcgtcac taccacgagc gtgctgccgc cggacgcgaa
     1981 cacgcgcagg cgctcgagcc cgggactcag cgcatgccct cgcgtcgggt ccaggaccgc
     2041 gagcgcttcg aggagctcgg gtcgcgcgag ggtccgcggc ggcgtctcgg aggtcgcgag
     2101 gcgtacgccg tagcccgacc ggacgtagag ctcgccgacc gacgcggctg cggagacgcc
     2161 gcgctcgaag gcctccggcg tcgctgcgag cccgcggcgt ctcgtgtcca cgaacagggc
     2221 gatgaccgcg ggccgggccg cctgctcctg gcggatcatg agctcgccgg ttcgcgcgac
     2281 gctcggccag tgaatcctgc gaaggtcgtc gccgacctcg taggcgcgca tcgtgtagaa
     2341 gtcctcgccg gtcctgaaca gctggcggct ccgcgagtcc cccctgccgc ctggaggcgc
     2401 cgggacgagc gctcggtcca ggtcctcgac ctccggatac acgacgagtt cgtgacgctc
     2461 cgtgaaccgc agctgccggc gaacgaggtc gaacgggtcg gagatcgatg cgctgagggg
     2521 tccgatcacg tgccgtccac gcgtgcggca cgtgacgtgg tacgagaacc gctcgctgcc
     2581 ctgtgccgga accgacggga ggaccgcgcg cgccggcggt ccgagcgacg ccggcagccg
     2641 gtcctcgagc agcaccaggg acgtgcggag gccgccgcgg ttctcgacgt cgacgtcgac
     2701 gcggacgcgc gcccccggga acgcccgcgg cgtcgagatc cgtcgcgtgg cgcggaggtc
     2761 ccgggggagc gagcgcatca ccagcaccgc gagcacgagg agcgcgagaa ggccgaccgc
     2821 gacgatgtgt atctcggccg agccgacgac ccgcgagccg agccagagga gcgtagcccc
     2881 cgtgagcacc gcagcggcac gcgccgtgag cacgcttcag cccttgacgg ggaccgggac
     2941 gtcgtcgagg acgcttccga gcacgacgcg cgcgtccctc cccgagagcc cggcgtcggc
     3001 ggacacgatc agccgatgcg cgaggatggg gaccgcgatc cccttgacgt cgtccgggac
     3061 cacgtagtcg cgtccatcgc tcgcggcgaa cgctcgtgag gcgcgaagga gcatgatcgc
     3121 ggcccgcgga ctggcgccga ggtagatgtc gggatggcgg cgcgtcgcct cggcgatccc
     3181 cacgatgtag cggcggatcg cgtcggcgac gtgtacctcg cgggccagcg cgatgaggcg
     3241 cccgacgctc gcggcgtccg tcacgggctc gacgtcgaac gtggccgacc cggttccgtg
     3301 cgtcgagagg atgtccatct cggcgtcggc gcccggatag ccgacctcca gacgcatcat
     3361 gaagcggtcc agctgggatt cggggagcgg gtacgtcccc tcgtgctcga tcgggttctg
     3421 cgtcgcgatc accatgaagg gtgtcccgag ctggtacgag atgccgtcga ccgtcacctg
     3481 ccgctcctcc atgctctcga ggagcgcgga ttgcgtcttg ggactcgcgc ggttgatctc
     3541 gtcgccgagg acgacgttcg cgaagatcgc gccgggcttg aactcgaagt cgccgcgctc
     3601 ctggttgaag acgttgacgc cgttgatgtc ggtcgggagc aggtccggcg tgaactggat
     3661 ccggcggaac gagcagtcga tcgaccgggc catcgccttg gcgagcatgg tcttcccgac
     3721 gccggggacg tcctcgatca ggaggtggcc ttcggcaacc agtgtcacga gcgcgagacg
     3781 gatctcgcgc ggttttccct ggatcgcgcg ttcgacgttc gtgtggatac ggtcgaagag
     3841 cgcctggaac catccccacg cgttcggctt ctgcaaccga tgcctccgac tccgagcgac
     3901 gcctcgccga ccggcccatc ttacggcggc cgtcctcgtt cggacggttc ggtgcggtcg
     3961 tgcgttcccg cggagacgcg gcggcggcgt gaggccgacc gagacg
//
LOCUS       DASZOB010000312         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_56982, whole genome shotgun sequence.
ACCESSION   DASZOB010000312 DASZOB010000000
VERSION     DASZOB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56982"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..277)
                     /locus_tag="VG709_05060"
                     /pseudo
     CDS             complement(1..277)
                     /locus_tag="VG709_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011127590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            308..565
                     /locus_tag="VG709_05065"
     CDS             308..565
                     /locus_tag="VG709_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32480.1"
                     /translation="MATFHSTLPRGARVKVEQGASLEEIVVGPARENDGTLNMMGALR
                     TSMATCGYENVHAFQRAEIMLAPAIKTEGKFLQQAQRLGMA"
     gene            642..2219
                     /gene="guaA"
                     /locus_tag="VG709_05070"
     CDS             642..2219
                     /gene="guaA"
                     /locus_tag="VG709_05070"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWC32481.1"
                     /translation="MEAVVELPVPEPRPEERPVLVVDLGGQYSQLIARRIRECRVYSE
                     LVPHTAPASLLRARRPRALVLSGGPASVYADGAPSVDPALFDLGVPVLGICYGMQLMA
                     QELGGRVERTGRSEFGRTELRTERESALFAGLPGEQTCWMSHRDSVVEPPAGSRVVAG
                     SPAASISAFEAAERRLYGVQFHPEVVHTPHGQDLLKNFLYRVAEAPPVWTAAAVIEEE
                     TARIRAQVGRERVLCALSGGVDSAVAALLAHKAVGDQLACVFVDHGLLRKDEAEQVAA
                     TFAGHFHVPLLHVDARDRFLARLRGVTDPEEKRRRVGEEFIRVFEEEAGRLGRVRYLV
                     QGTLYSDVIESGGGEGPASTIKSHHNVGGLPEEMDLDLVEPLRMLFKDEVRRVGEELG
                     LPEAMVWRQPFPGPGLAIRIIGDVTEERLAILRDADAIVQDEVRRAGLYRDLWQSFAV
                     LPAIRSVGVQGDERTYAYPIVIRAVTSDDAMTADWARLPYDLLETISSRIINEIPGVN
                     RVALDVSSKPPATIEWE"
     gene            complement(2429..2713)
                     /locus_tag="VG709_05075"
     CDS             complement(2429..2713)
                     /locus_tag="VG709_05075"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008527828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWC32482.1"
                     /translation="MRDVKRKVRRRVVVHGRVQGVFFRDSLRRLADQRGVAGWAENAP
                     DGTVKAVFEGEPDAVDRLVAFCHDGPRGAQVARVDVADDDPEDLTGFSIR"
     gene            complement(2806..>3894)
                     /locus_tag="VG709_05080"
     CDS             complement(2806..>3894)
                     /locus_tag="VG709_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32483.1"
                     /translation="GAMLVALIPNVARGLETSVTLVAAAITAYMVPFATLQLVSGTAA
                     ERLGARRVVRAGYVVFGAGALACALAPEIWTFVAARAIMGAANAFLSPILLAALSEVV
                     APGVLGRTVGTFAAWQTAGFTLAPAIGGALGEVSWRLAFALVTVAALLLAFPRRTLGS
                     FESAARADATLRALLNRWIALLSAKALLGYLGFTAIGFVLVLVAASEFGLGSAARGLL
                     VAGYGLGGILLGRFAGSIVDRAGRAPTALAGALASAAGVLALAFVPNVWSFALVWLAI
                     GCTATFVWAGLNTIAVESFPANRAGATSAYSAFKFAGIAVGPLIYLPLFHVDTRAPFL
                     LAAGFSLLLAALVLPWFGRYRPPRETRT"
BASE COUNT          567 a         1413 c         1399 g          515 t
ORIGIN      
        1 cgtcgttctc gcgggcagga ccgacgacga tctcctcgag cgacgccccc tgctccacct
       61 tgacgcgcgc cccgcgcggc agcgtgggat ggaacgtcgc catgccccag tggtagccgc
      121 gcccgggcgc ctcgcgcgcg cgcgccagcg gcgagccgat catgacggcg tccgccccgc
      181 acgcgatcgc cttcgcgatg tcgccgcccg tccgcatacc gccgtcggcg atcacgttca
      241 cgtagcggcc cgtgtcgaga atgtgctgcg cgcgcgctag gcgcccgggc gcggctacca
      301 ctggggcatg gcgacgttcc attccacgct gccgcgcggg gcgcgcgtca aggtggagca
      361 gggggcgtcg ctcgaggaga tcgtcgtcgg tcctgcccgc gagaacgacg gcacgctgaa
      421 catgatggga gcgctgcgga cgtcgatggc gacctgcggc tacgaaaacg tccacgcctt
      481 ccagcgcgcc gagatcatgc tcgccccggc gatcaaaacg gaggggaagt tcctccagca
      541 ggcccagcgc ctgggcatgg cgtgagccgc cggatcgcgg ggagacgcgg ttcccgcacg
      601 ggcccctgcc tctcacgctc gccgctcggg gctcggaccc ggtggaagcg gtagtcgagc
      661 ttcccgtccc cgagcctcgg cccgaggagc ggccggtgct cgtcgtcgac ctcggcggcc
      721 agtactcgca gctgatcgcg cggcgcatcc gggaatgccg ggtctactcc gagctcgtgc
      781 cgcacacggc gcctgcgtcg ctccttcgcg cccgccgccc gcgtgcgctc gtcctctccg
      841 gcggcccggc gtcggtctac gcggacggcg cgccctcggt cgacccggcg ctcttcgacc
      901 tcggcgttcc cgtgctcggc atctgctacg gcatgcagct catggcgcag gagctcggcg
      961 gacgcgtcga gcggaccggc cgctcggagt tcgggcggac cgagcttcgg acggaacgcg
     1021 agtccgcgct cttcgccggc cttccggggg agcagacgtg ctggatgagc caccgcgact
     1081 cggtggtcga gcctcccgcc ggctcgcgcg tcgtcgcggg gtcgcccgcg gcgtcgatct
     1141 cggccttcga ggcggccgag cggcgcctct acggcgtcca gttccacccg gaggtcgtcc
     1201 acactcccca cgggcaggac ctgctcaaga acttcctcta ccgcgtcgcc gaggcgccgc
     1261 cggtgtggac ggccgccgcg gtgatcgagg aggagaccgc gcgcattcgc gcgcaggtcg
     1321 gtcgcgagcg cgtcctctgc gcgctctccg gcggcgtcga ctctgccgtc gccgccctgc
     1381 tcgcgcacaa ggcggtggga gaccagctcg cgtgcgtctt cgtcgatcac ggcctcctgc
     1441 gcaaggacga ggccgagcag gtcgcggcca cgttcgcggg gcacttccac gtgccgctcc
     1501 tccacgtcga cgcgcgcgac cgcttcctcg cgcgcctacg cggcgtcacc gatcccgagg
     1561 agaagcggcg ccgcgtcggg gaggagttca tccgcgtctt cgaggaggag gccgggcggc
     1621 taggacgcgt ccgctacctc gtccagggga cgctctactc tgacgtgatc gagtccggcg
     1681 gcggcgaggg ccccgcgtcg acgatcaagt cgcaccacaa cgtgggcggc ctgcccgaag
     1741 agatggatct cgacctcgtg gagccgctta ggatgctctt taaggacgag gtccgccgcg
     1801 tcggcgagga gctcggcctc cccgaggcga tggtgtggcg tcagccgttc ccgggtcccg
     1861 ggctcgccat ccgcatcatc ggcgacgtga ccgaggagcg gctcgcgatc ctccgtgacg
     1921 ccgatgcgat cgtgcaggac gaggtccgcc gcgccggcct ctaccgcgac ctctggcaga
     1981 gcttcgccgt gctgccggcg atccggtccg tcggggtcca gggcgacgag cggacgtacg
     2041 cgtacccgat cgtgatccgc gcggtcacgt cggacgacgc gatgacggcc gactgggcgc
     2101 gcctgcccta cgacctcctc gagacgatct cgagccgcat catcaacgag attccaggcg
     2161 tgaaccgcgt cgcgctcgac gtgagctcga agccgcccgc gacgatcgag tgggagtaga
     2221 gcgcggcgtt cgtaagactt ttgtaaatgc cccggaccgg gggtatgcca aatgtccccg
     2281 cgccgcgatg atgaatgccg gagcgacgtt cgccgatcga agccgcagcg gtcgccgaaa
     2341 tagcctcgct ctcttggggg gcgagtcccg ggccgcaggg gactcgccct ctttctttct
     2401 ctcccgcagc ccgaccggcg cgcgccgcct agcggatcga gaacccggtt agatcctccg
     2461 ggtcgtcgtc ggcgacgtcg acgcgcgcca cttgggcacc gcgcgggccg tcgtggcaga
     2521 acgcgacgag acggtcgacg gcgtccggct caccttcgaa gaccgccttc accgtcccgt
     2581 cgggggcatt ctccgcccac ccggcgacac cgcgctggtc ggcgaggcgc cgcagcgagt
     2641 ctcggaagaa cactccctgc acgcggccat gcacgacgac tcgccttcgg accttgcgtt
     2701 tcacatcacg cacgccccgt cggtcgtacg cgccgccgtc cgccgtcatc gtggcgccgc
     2761 ggcggctcgt gacgtgaaac acaagtcggg acgcggtcgg cgcgatcacg ttcgcgtttc
     2821 ccgcggtggg cggtagcgcc cgaaccaggg gaggacgagg gcggcgagga gcagcgagaa
     2881 cccggccgcc aggaggaacg gggcgcgcgt gtcgacgtgg aagagcggca ggtagatgag
     2941 cggcccgact gcgatgcccg cgaacttgaa cgcgctgtag gccgacgtcg cgcctgcccg
     3001 gttcgcgggg aaggactcga cggcgatcgt gttgagcccg gcccagacga acgtcgccgt
     3061 gcacccgatc gcgagccaga cgagggcgaa gctccacacg ttcgggacga acgcgagcgc
     3121 gagcacgccg gctgcactcg cgagcgctcc ggccagcgcg gtcggagcgc gcccggcccg
     3181 gtcgacgatc gagccggcga agcggccgag cagaatgccg ccgagcccgt acccggcgac
     3241 gagcaggccg cgcgcggccg aaccgagccc gaactcgctc gccgccacga ggacgagaac
     3301 gaagccgatc gccgtgaagc cgagatagcc cagcagcgcc ttggcggaga gcagtgcgat
     3361 ccagcggttc agaagcgcgc gcagcgtcgc gtcggccctc gccgcgctct cgaacgaccc
     3421 gagcgtccgg cgcgggaacg cgaggagaag cgccgcgacg gtgacgagtg cgaacgcgag
     3481 gcgccacgag acctcgccga gcgccccgcc gatcgcgggc gcgagcgtga agcccgccgt
     3541 ctgccaggcg gcgaaggttc cgaccgtgcg cccgagcacg ccgggcgcga cgacctccga
     3601 gagggcggcc aggaggatcg ggctcaggaa cgcgttcgcc gcacccatga tggcgcgggc
     3661 ggccacgaac gtccagatct ccggggcgag cgcacatgcg agcgcccccg ctccgaagac
     3721 gacgtatccg gcccggacga cgcgccgcgc gccgagccgc tccgcggcgg tgccggagac
     3781 gagctggagc gtggcgaacg ggaccatgta cgccgtgatc gcggctgcca cgagcgtgac
     3841 cgacgtctcg aggccgcggg cgacgttcgg gatcagcgcg acgagcatcg cgcc
//
LOCUS       DASZOB010000313         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_107945, whole genome shotgun sequence.
ACCESSION   DASZOB010000313 DASZOB010000000
VERSION     DASZOB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107945"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VG709_05085"
     CDS             complement(<1..692)
                     /locus_tag="VG709_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC32484.1"
                     /translation="MADFVWRPSAEVLEQANVVRLMRRHALDDYWELVRRSQEDPEWF
                     WPAAIEDMGLEFSQPWERVVDLSRGPEWATWFVGGRVNIAWNCVHRWAHGERAGSTAT
                     VFAGEDATRRELTFADMSREVTRLAEGLASLGVGEGDCVAIFLPMSPEVAIASHACAH
                     LGAVQVPIFSGFAAPAVAARLRDSEAKVVITADGSLRRGRELAMKEVVDEAAAGSSAV
                     DHVVVWRRLDRD"
     gene            complement(761..>1511)
                     /locus_tag="VG709_05090"
     CDS             complement(761..>1511)
                     /locus_tag="VG709_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC32485.1"
                     /translation="LVFLGNPGTGKTTIARLLAETYRATGLLRRGHLVEVDRAGLVGQ
                     WVGSTALKTDRVVRQALDGVLFIDEAYALATDPERTQTDFGAEAIETLLKRMEDFRER
                     LVVIVAGYPRLMERLLASNPGLRSRFAREIVFPDYSTQELLGITLKFVGEHEYALEPA
                     AEATLRAIFDSPPRDEGFGNARFARTMFEQALNMHALRLARSGVREPSLAELQTLTDE
                     DVRAAARALGEEPPADVAGARGLFRRRRQAS"
BASE COUNT          228 a          533 c          545 g          205 t
ORIGIN      
        1 tcgtcgcggt cgagccgccg ccagacgacg acgtggtcga cggcggacga gccggccgcg
       61 gcctcgtcga cgacctcctt catcgcgagc tcgcgtccgc gccgaagcga cccgtccgcc
      121 gtgatgacga ccttcgcctc cgagtcgcga aggcgggcgg ccacggccgg tgcggcgaag
      181 cccgagaaga tcggcacctg gacggcgccg aggtgcgcgc acgcgtgcga ggcgatcgcg
      241 acctccggag acatgggcag gaagatcgcg acgcagtcgc cctcgccaac gccgagcgag
      301 gcgagcccct cggccagccg ggtcacctct ctcgacatat cggcgaacgt gagctcgcgg
      361 cgcgtcgcgt cttcgcccgc gaagacggtg gccgtcgagc ccgctcgctc cccgtgcgcc
      421 caccggtgga cgcagttcca ggcgatgttc acgcggcccc cgacgaacca cgtggcccat
      481 tccggcccgc gcgagaggtc gacgacgcgt tcccacggct gcgagaactc gaggcccatg
      541 tcctcgatcg cggccggcca gaaccactcg gggtcctcct gcgagcggcg cacgagctcc
      601 cagtagtcgt cgagggcgtg gcggcgcatc aaccggacga cgttggcctg ctcgagtacc
      661 tccgcggacg gccgccagac gaaatccgcc acgcctcgca gtctaggagg gtttgacgtc
      721 ctccgacgac gagcgacgtt tcgccaatcc gtcctactag ctaggaagct tggcgccggc
      781 ggcggaagag gccgcgtgcc cccgcgacgt cggcgggcgg ctcctcacct agtgcgcgcg
      841 cggccgcacg gacgtcctcg tcggtgagcg tctggagctc ggccagggac ggctcgcgta
      901 cgccgcttcg cgcgaggcgg agcgcgtgca tgttgagggc ctgctcgaac atcgtccgcg
      961 cgaagcgggc gttcccgaac ccctcgtcgc ggggagggga gtcgaagatg gcccggaggg
     1021 tcgcctcggc ggccggctcg agcgcgtact cgtgctcgcc gacgaacttg agcgtgatac
     1081 cgagcagctc ctgcgtcgag tagtcgggga agacgatctc ccgcgcgaag cgggaacgga
     1141 gacccggatt ggacgcgagc aggcgctcca tgaggcgtgg gtagccggcg acgatgacga
     1201 cgaggcgctc gcggaagtcc tccatccgct tcagcagggt ctcgatcgcc tcggctccga
     1261 agtcggtctg cgtccgctcg gggtcggtcg cgagcgcgta ggcctcgtcg atgaagagca
     1321 cgccgtcgag ggcctggcgg acgacccggt cggtcttcag cgccgtcgag ccgacccact
     1381 ggccgacgag ccccgcacgg tccacctcga cgagatgacc gcgccggagg agccccgtcg
     1441 cgcggtacgt ctctgcgagg aggcgggcga tcgtcgtctt gccggtgccc ggatttccga
     1501 ggaacacgag g
//
LOCUS       DASZOB010000314         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_127554, whole genome shotgun sequence.
ACCESSION   DASZOB010000314 DASZOB010000000
VERSION     DASZOB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127554"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..396
                     /locus_tag="VG709_05095"
     CDS             52..396
                     /locus_tag="VG709_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWC32486.1"
                     /translation="MLLVGAIVLALFVLPRDWGVAVVAGAAVVEVAETFLWIKLSRRR
                     RVQMGPETLVGARGEVVSACRPDGRARVQGEVWQARCAAGADAGETVRVLAREGLVLV
                     VEPDPGGAARAE"
     gene            478..1770
                     /locus_tag="VG709_05100"
     CDS             478..1770
                     /locus_tag="VG709_05100"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWC32487.1"
                     /translation="MTSESATRHRPDLPPELDAPLFRTARGQLDGVLEVAGVTPYAGE
                     KLRYPERSLAVSVPVRLDDGSLAVYQGYRVQHSSVLGPTKGGVRYDAAVTLGECAALA
                     MWMTWKCALLRLPYGGAKGGVRCDPRVLSQHEREGLTRRFTSELLPIIGPQRDIPAPD
                     MATDEQTMAWMMDTYSMQVGYAVPEIVTGKPLAVGGSALRPEATGIGVVTVVEEAVAR
                     LGWPLDGASCVVQGFGKVGGVAAHELAARGARVVAVSDISGGVHARKGLDLGALEVWR
                     TAEGGVAGFPDGDEVTNAELLELPCDVLVLAATERQVTAENAPRLATRLVAEGANGPT
                     TLTADDILAERGITVLPDILANAGGVAVSYFEWVQDIQRLFWGSDEIRTRLGERMRGA
                     RARVWALSEREALSLRSAALVASIREVARALEVRGVYP"
     gene            1767..>2044
                     /locus_tag="VG709_05105"
     CDS             1767..>2044
                     /locus_tag="VG709_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWC32488.1"
                     /translation="MKVRAALVRDPRVVPAEAGAREVAELLTHPNVRSALVVDGGRLV
                     GCVTMESIVAAVARGADVGRLSARDLADGDEVTNAELLELPCDVLVLAA"
BASE COUNT          253 a          708 c          783 g          300 t
ORIGIN      
        1 atagtgacgc gttgggcatt cttcaccagc ccggactaac gtgggcgccg gatgcttctt
       61 gtcggtgcga tcgtcctcgc gctcttcgtc ctcccgcgcg actggggagt cgccgtcgtc
      121 gccggggcgg cagtcgtcga ggtggcggag accttcctct ggatcaagtt gtcgcggcga
      181 aggcgcgtcc agatggggcc ggagaccctc gtcggcgccc gcggcgaggt cgtgtccgcg
      241 tgccgccccg acggccgggc gcgcgtgcag ggagaggtct ggcaggcccg ctgcgccgcg
      301 ggggcggacg ccggagaaac cgtccgcgtc ctcgcccggg aaggtctcgt gctcgtcgtc
      361 gagcccgatc cggggggcgc ggcgagagcc gagtagaaga gcccgggctt cgtgcgcccc
      421 gagcgaccgc ggcacgctgc tcgccccgat gctgctcgtc gtcctagact ccgcgccgtg
      481 acgagcgaat cggcgacgag gcaccgaccg gatctgccgc ccgagctcga cgctccgctc
      541 tttcggacgg cgcggggcca actcgacgga gtcctcgagg tcgccggcgt gacgccctac
      601 gcgggggaga agctccgcta tccggagcgt tcgctcgcag tctcggtccc cgtgcggctc
      661 gacgacggct cgctcgcggt gtaccagggc taccgcgtgc agcactcgag cgtcctcggc
      721 ccgacgaagg gcggggtccg ctacgacgcc gccgtcacgc tcggcgagtg cgcggcgctc
      781 gcgatgtgga tgacgtggaa gtgcgcgctc cttcggcttc cgtacggtgg tgcgaagggc
      841 ggagtccgct gcgatccgcg cgtgctctcg cagcacgagc gggaagggct cacccgtcgc
      901 ttcacctccg agctgctgcc gatcatcggc ccgcagcggg acattccggc gcccgacatg
      961 gcgaccgacg agcagacgat ggcgtggatg atggacacgt actcgatgca ggtcgggtac
     1021 gcggtcccgg agatcgtcac cggcaaaccg ctcgcggtcg ggggctccgc cctgaggccg
     1081 gaggcgaccg ggatcggcgt cgtgacggtc gtcgaggagg ccgtcgcgcg cctcggctgg
     1141 ccgcttgacg gggcgagctg cgtcgtgcag ggattcggca aggtcggcgg cgtcgccgcg
     1201 cacgagctcg ccgcgcgcgg ggcccgcgtg gtcgccgtct cggacatctc gggcggcgtg
     1261 cacgcgcgga agggcctcga cctcggcgcg ctcgaggtat ggcggacggc ggagggaggc
     1321 gtcgcgggct ttcccgacgg cgacgaggtg acgaacgccg agctcctcga gcttccctgc
     1381 gacgtcctcg tccttgctgc gacggagcgc caggtcacgg cggagaacgc gccgaggctc
     1441 gcgacgcggc tcgtggccga gggcgcgaac gggccgacga ccctcacggc ggacgacatc
     1501 ctcgccgagc ggggcatcac cgtgctcccg gacatcctgg cgaacgcggg cggggtcgcc
     1561 gtctcctatt tcgagtgggt gcaggacatc cagcgcctct tctggggctc cgacgagatc
     1621 cggactcggc tcggcgagcg gatgcgcgga gcgcgcgcgc gcgtgtgggc gctctccgag
     1681 cgcgaggcgc tttcgctccg aagcgccgcg ctggtggcca gcatccgcga agtcgctcgc
     1741 gccctcgagg tgcgcggggt gtacccgtga aggtgcgcgc ggcgctcgtg cgcgatcccc
     1801 gcgtggtgcc ggccgaagca ggcgcgcgcg aggtcgccga gcttctcacg cacccgaacg
     1861 tgcgcagcgc cctcgtcgtg gacggcgggc gtctggtcgg atgcgtgacg atggagtcca
     1921 tcgtcgcggc ggtcgcccgc ggggcggacg tcggccgcct gtcggcacgc gatctcgccg
     1981 acggcgacga ggtgacgaac gccgagctcc tcgagcttcc ctgcgacgtc ctcgtccttg
     2041 ctgc
//
LOCUS       DASZOB010000315         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_167996, whole genome shotgun sequence.
ACCESSION   DASZOB010000315 DASZOB010000000
VERSION     DASZOB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167996"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1093
                     /locus_tag="VG709_05110"
     CDS             131..1093
                     /locus_tag="VG709_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWC32489.1"
                     /translation="MAEDDGRDGAREVPREGTVPESARHAGDVEESPLDLSASGWRAT
                     LKRTLHEVKRDRVPLTAAGVAFYWFLTVFPLVIAGVGMLALVNATDATVEAVQESIRS
                     VLPGDAAAVLTGAVQNAAGRAAGTGLVAAVVGIALAVWSASSGMAAAQVGLDVAYDVE
                     EDRTFLKKRAMGMVLVLVALLLGGIASALLVFGQPIGEAIREHVPGGAAFEIVWTVIR
                     WALAFLAVATLFALFYFIGPNRKPPSWKWLSPGGLLATLLWLGASFALSFYVSHFGGS
                     YAKTYGSLVGVVVLLLWLFVSALALLLGAELNGELERQRARRER"
     gene            complement(1131..1463)
                     /locus_tag="VG709_05115"
     CDS             complement(1131..1463)
                     /locus_tag="VG709_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWC32490.1"
                     /translation="MAKYLWRGSYTELGLKGLREDGGSRRRESVSKLAESLGGKLEAF
                     YFAFGEDDLYLIYEMPDNVSATAGALIVGAPGAVRGATIPLVTPEEVDEAVKRIGEYR
                     PPGGGTQG"
BASE COUNT          183 a          579 c          550 g          281 t
ORIGIN      
        1 cctctccggt gacctcgccc gcttctctga gcgtcatgcg ggcacgctat cgcgctccgc
       61 tcgttttcgt tcgcgtaccg cgggggtacc gtcgacggag gcgccgacct cgcgtggaag
      121 ggttcgtcca gtggctgaag acgacggccg agacggtgct cgcgaagttc ctcgcgaggg
      181 cacggtcccc gagtcggcgc gtcacgcggg cgacgtcgag gagtccccgc tcgatctctc
      241 ggcgtccggc tggcgggcga cgctgaagcg aaccctccac gaggtcaagc gggaccgcgt
      301 cccgctgacc gccgccggcg tcgcgttcta ttggttcctg acggtgttcc cgctcgtcat
      361 cgcgggcgtc gggatgctcg cgctggtgaa cgcgaccgac gcgacggtcg aggcggttca
      421 ggagtcgatc cggtcggtcc tcccgggtga cgcggcggcg gtcctgaccg gtgccgtgca
      481 gaacgcggcg ggccgagccg ccggaaccgg gctcgtcgcc gcggtcgtcg gtatcgcgct
      541 cgccgtctgg agcgcttcgt cgggcatggc ggccgcccag gtggggctcg acgtcgccta
      601 cgacgtggag gaggaccgga ccttcctgaa gaagcgcgcg atgggcatgg tcctcgtgct
      661 cgtcgcgttg ctgctcggcg gcatcgccag cgcgctcctc gtcttcggcc agccgatcgg
      721 cgaggccatc cgggagcacg tcccgggcgg cgccgccttc gagatcgtgt ggacggtgat
      781 ccgttgggcg ctcgcgttcc tggccgtggc gacgctcttc gcgttgttct acttcatcgg
      841 ccccaaccgc aagccgccca gctggaagtg gctctcgccg ggcggattgt tggcgacgct
      901 gctgtggctc ggcgcttcgt tcgccttgtc gttctacgtc tcgcacttcg gtggttcata
      961 cgcaaagacc tacggctcgc tcgtcggcgt cgtcgtcctg ttgttgtggc tcttcgtctc
     1021 cgccctcgcg ctgctgctcg gcgccgagct gaacggcgag ctcgagcggc aacgtgcccg
     1081 acgcgaacgc tgactccccg tgcgcggcgc tggcgctccg ggcccgcagg ctagccctgg
     1141 gtcccgccgc cgggcggccg gtactcgccg atgcgcttga ccgcctcgtc gacctcctcc
     1201 ggcgtgacga gcgggatcgt cgccccgcgc acggcaccgg gcgccccgac gatcagcgcc
     1261 ccggccgtcg ccgaaacgtt gtcgggcatc tcgtagatga ggtacaggtc gtcctccccg
     1321 aaggcgaagt agaacgcctc gagcttgccg ccgaggctct cggcgagctt ggagacggat
     1381 tcgcgccgcc tgcttccgcc gtcttccctc aggcccttca ggccgagctc ggtgtacgag
     1441 cctctccaca ggtacttcgc cacgacgcgc ctccttcggt tcggctccgg cacatcctag
     1501 gcacgccctg cgcggccgcg cacgtcgacc gtgacggccc actcacccgc tcgggcgacg
     1561 gagccccacc ccggtgatcc cgaggatcgc ccg
//
LOCUS       DASZOB010000316         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_108080, whole genome shotgun sequence.
ACCESSION   DASZOB010000316 DASZOB010000000
VERSION     DASZOB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108080"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VG709_05120"
     CDS             complement(<1..953)
                     /locus_tag="VG709_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWC32491.1"
                     /translation="MAERSLYDVLGVPREASQDDIRKAFRRLARQYHPDVNADPEAEQ
                     RFKEINLAYETLSDPAKRRQYDVYGGETFTPDMFGFMGDIGDLFEAFFGAPFGARTRT
                     RRRTRTHRGADVRVVLDLTFEEAAFGTERDIDVQTLETCERCGGNGSEPGTSPTRCRE
                     CGGSGQLSDVRRSVFGTVMTSRPCGTCEGTGEEIAEPCRECGGDGRLPKRQVMSVKVP
                     PGVADGMDLRVEGGGQDGRHGGYSGDLYIAVRVEPHRLFERLGQDLLTVLELPVAVAL
                     LGGEVEIETLEGPRPLKVPAGTRPGTALRLRGAGLPHLGRRG"
     gene            complement(962..2002)
                     /gene="hrcA"
                     /locus_tag="VG709_05125"
     CDS             complement(962..2002)
                     /gene="hrcA"
                     /locus_tag="VG709_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWC32492.1"
                     /translation="MPTAAGLDPRKSSILRAVVAGYIRTAEPVGSGAIARRYRLGVSP
                     ATIRNELSVLEEMGYLSHPHTSAGRIPTDRGYRFYVDTLPRRPALSETHRRAIASFFG
                     ELQPDVEEMLRRTASLLSRVTRYAAIAVAPVPGSTTVARADVVEVGGGWLLLLIGDTG
                     RVDKRVLDIPREVHAAVVRRAGRALTQAVGGRSFAEARSRVERLGVTAGDERPVFEAA
                     ARALCDLEERETPDHVFLGGAANIAGEGAFERRETVRRLFEALEQQEALHHFLRAASR
                     EGDVAVRIGHEHPLAAMREASTVVAPYRAGGRPAGTVAVIGPTRMAYPTAISVVDTVA
                     RRLSRLIESRAE"
     gene            complement(2024..>2937)
                     /gene="hemW"
                     /locus_tag="VG709_05130"
     CDS             complement(2024..>2937)
                     /gene="hemW"
                     /locus_tag="VG709_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWC32493.1"
                     /translation="PTTLRADELGALITAFTALFDLQPGAEVTCEANPDTVDVAYLEA
                     LRAGGVERLSIGVQSFDRAVLEALDRVHPASSARRAFAAARRAGFTNVSVDLIYGARG
                     ETLPSWVRTLDEAIALRPEHLSCYALTVEPNTRLGRSVATGTLVPPEPDLQADMYEAA
                     CERLAAAGYRHYEVSNWAFPGFESRHNLGYWEGRAYLGLGAGAHSFRSDRRWWNVRPP
                     ERYLALVEDGRPPLGGGERLTEQEARLEHLLLALRRADGLPVEEVPPDVAASFAERGL
                     SVLEGDRFLPTEPGMLLANEMVLALSP"
BASE COUNT          406 a         1084 c         1069 g          378 t
ORIGIN      
        1 ccccggcggc cgaggtgcgg gaggccggcg ccgcgcagcc ggagcgccgt ccccggacgc
       61 gtccccgccg gcaccttgag cgggcgaggg ccttcgagcg tctcgatctc gacctcgccg
      121 ccgaggagcg cgaccgcgac cgggagctcg agcaccgtga gcaggtcctg tccgaggcgc
      181 tcgaagagac gatgcggctc gacacggacc gcgatgtaga ggtcacccga gtagcctccg
      241 tgccggccgt cctggccgcc gccctcgacg cggaggtcca tgccgtccgc gacgccgggg
      301 ggcaccttga ccgacatcac ctgtcgcttg gggagccgtc cgtcgccgcc gcactcgcgg
      361 cacggctccg cgatctcctc ccccgtgccc tcgcacgtcc cgcacggtcg cgacgtcata
      421 acggtcccga acacgctgcg gcggacgtcg gatagctgcc cgctgccgcc gcactcccgg
      481 cagcgggtcg gggacgtgcc cggctcggag ccgttgccgc cgcatcgctc gcaggtctcg
      541 agcgtctgga cgtcgatgtc cctctcggtc ccgaacgccg cctcctcgaa cgtcaggtcg
      601 agcacgaccc ggacgtcggc tcctcgatgg gttcgcgttc gccggcgcgt ccgggtccgc
      661 gcgccgaacg gtgccccgaa gaacgcctcg aacaggtcgc cgatgtcccc catgaagccg
      721 aacatgtcgg gggtgaacgt ctcgccgccg tagacgtcgt actgacgccg cttcgcgggg
      781 tcggacagcg tctcgtaggc gaggttgatc tccttgaacc gctgctcggc ctcggggtcc
      841 gcgttgacgt cggggtggta ctgccgcgcg agccggcgga acgccttgcg gatgtcgtcc
      901 tggctcgcct cacgtgggac gcccaggacg tcgtacagcg agcgctcggc catggcgcga
      961 gctactccgc gcgcgactcg atcagccgcg agagccgccg cgccaccgtg tcgacgaccg
     1021 agatcgcggt cgggtacgcc atgcgggtcg ggccgatgac cgcgacggtc cccgcggggc
     1081 ggccgccggc gcggtacggc gccacgacgg tgctggcctc gcgcatcgcg gcgagcgggt
     1141 gctcgtggcc gatccggacc gcgacgtcgc cctcgcgcga ggcggcgcgg aggaaatggt
     1201 ggagcgcctc ctgctgctcg agcgcctcga agagccgacg gacggtctcc cgccgctcga
     1261 acgccccctc gccggcgatg ttggccgcgc cgccgaggaa gacatggtcc ggcgtctcgc
     1321 gctcctcgag gtcgcacagc gctcgcgcgg cggcctcgaa caccggccgc tcgtcgccgg
     1381 cggtcacccc gagccgctcg acgcgcgagc gagcctcggc gaacgaccgt ccgccgaccg
     1441 cctgcgtgag agctcgcccg gcgcggcgga cgacggccgc gtggacctcg cgcgggatgt
     1501 cgagcacccg cttgtcgacg cgccccgtgt cgccgatgag cagcagcagc cagccgccgc
     1561 cgacctcgac gacgtccgca cgtgcgacgg tcgtcgagcc cggcaccggc gcgacggcga
     1621 tcgcggcgta gcgcgtcacg cgcgacagca gcgaggcggt ccggcgcagc atctcctcga
     1681 cgtccggctg cagctctccg aagaacgacg cgatggcgcg acggtgggtc tccgagagcg
     1741 ccgggcggcg cggcagcgtg tcgacgtaga agcggtaccc ccggtcggtc gggatgcggc
     1801 cggccgacgt gtgcggatgc gagaggtatc ccatctcctc gagcaccgag agctcgttgc
     1861 ggatcgtcgc cgggctcacg cccagccggt agcgcctcgc gatcgcgccg ctcccgaccg
     1921 gctccgccgt acggatgtaa cccgcgacca cggcgcgcag gatcgaggac ttccgaggat
     1981 cgagaccagc cgcggtaggc atcgcacccc agtattagca ctctcaaggg gagagtgcta
     2041 ggaccatctc gttcgcgagc agcatcccgg gttcggtcgg gaggaaccgg tctccctcca
     2101 gcacgctcag cccgcgctcg gcgaatgagg ccgcaacgtc gggcgggacc tcctcgaccg
     2161 gaagaccgtc ggcacggcgg agcgccagga ggaggtgctc caagcgcgcc tcttgctcgg
     2221 tgagtcgctc gccgccgccg agcggcgggc ggccgtcctc gaccagcgcg aggtagcgct
     2281 ccggcgggcg gacgttccac caccgccggt ccgagcggaa cgagtgcgcc ccggcgccga
     2341 ggccgaggta cgcgcgccct tcccagtaac cgaggttgtg acgtgactcg aaccccggga
     2401 acgcccagtt cgagacctcg tagtgccggt accccgccgc ggcgaggcgc tcgcaggccg
     2461 cctcgtacat atcggcctgg agatcgggct cgggtggcac cagggtgccc gtcgcgaccg
     2521 agcgcccgag ccgcgtgttc ggctcgacgg tgagcgcgta gcacgagagg tgctcggggc
     2581 ggagggcgat cgcctcgtcg agcgtgcgga cccacgacgg cagcgtctcg ccgcgcgcgc
     2641 cgtagatgag gtcgacggac acgttcgtga accccgcgcg tcgcgccgcc gcgaacgctc
     2701 ggcgcgcgga cgacgcggga tggacgcgat cgagcgcctc gaggacggcg cggtcgaacg
     2761 actggacgcc gatcgagagc cgctcgacgc cccccgcgcg aagcgcctcg agatacgcga
     2821 cgtcgacggt gtccggattc gcctcgcagg tgacctcggc ccccggctgg aggtcgaaca
     2881 gcgctgtgaa cgccgtgatc agggcgccga gctcgtcggc acgcagggtc gtcggcg
//
LOCUS       DASZOB010000317         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_174881, whole genome shotgun sequence.
ACCESSION   DASZOB010000317 DASZOB010000000
VERSION     DASZOB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174881"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VG709_05135"
     CDS             <1..215
                     /locus_tag="VG709_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32494.1"
                     /translation="IPGGFEQLERAADDVDLDGFSVRVASIDDLIRMKRAAGRPKDLI
                     EVEVLGALRDEIDAQAIEARRRRRRR"
     gene            224..919
                     /locus_tag="VG709_05140"
     CDS             224..919
                     /locus_tag="VG709_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32495.1"
                     /translation="MRDQHFEALLSSRGYVLGETEEHYGIWHRRARGEPLERFPLTAE
                     GFDAAKARFRELTRRRRDPERVRRALWWIVLVGVTMWVAETLFSAVVYGTTLTGIVPR
                     VVAQIAFALGAVGYRLAVGGLFVLAALFLVRNDTRPRVASTDTPESPPRGWETIAWWA
                     ASLGLGIWIVSVLATRTLAPDDPALFLFEGRPPRPLYLAASLIEGIAFRTWVAAAVLL
                     ALARLYGLRRLRP"
     gene            1056..2105
                     /gene="recA"
                     /locus_tag="VG709_05145"
     CDS             1056..2105
                     /gene="recA"
                     /locus_tag="VG709_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWC32496.1"
                     /translation="MLDITLQQIEKQHGKGAVMKLGEHPAGQATAVIPTGSLALDVAL
                     GIGGIPRGRVVEVFGPEGSGKTTVCLHIIAEAQARGGIAAFVDAEHALDPTYARTLGV
                     NIDELLVSQPDSGEQALEIGDMLVRSGALDLLVIDSVAALVPRAEIEGEMGDAHVGLQ
                     ARLMSQAMRKLSGSLSRFDTTAIFINQLREKIGIMFGNPETTPGGRALKFYSSVRLDV
                     RKIDNLKDGTDVVGSRVRTKVVKNKLAPPFRQAEFDIVYGKGISKEGSLLDVGVDTEI
                     VRKSGAWFTYEGEQLGQGRENARQFLQEHKEIAAEIERRVREAVGLSAFGDADEPVPD
                     VEAADDKERAKAATG"
BASE COUNT          370 a          779 c          864 g          357 t
ORIGIN      
        1 ggatcccggg aggctttgag cagctcgagc gtgcggccga cgatgtcgac ctcgacgggt
       61 tcagcgtccg cgtcgcctcg atcgacgacc tgatccggat gaagcgagca gcgggcagac
      121 cgaaggacct catcgaggtc gaggtgttgg gtgcgctccg cgacgagatc gacgcgcagg
      181 cgatagaggc gcgaaggcgc cggcgtcgaa ggtagggatc ggagtgcgcg accagcattt
      241 cgaggcactg ctgtcgagcc gcggatacgt cctcggcgag acggaggagc actacgggat
      301 ctggcaccgg cgagcgcgcg gtgagccgct cgagcggttc ccgctgacgg cggaaggctt
      361 cgacgcggcc aaggcacggt tccgcgagct gacccgccgc cgccgcgacc ccgagcgcgt
      421 ccgccgcgcg ttgtggtgga tcgtgctcgt gggcgtgacg atgtgggtgg ccgagaccct
      481 gttcagcgcc gtggtctacg ggaccacgct caccgggatc gtccctcgcg tcgtcgcaca
      541 gatcgcattc gccctcggtg cagtcggata ccgactcgcc gtgggcggct tgttcgtcct
      601 tgccgcgctg ttcctcgtga ggaacgacac tcgcccgcgg gtcgcatcta ccgacacgcc
      661 ggagtcgcct cctcgaggtt gggagacgat cgcgtggtgg gctgcgagcc tcgggctcgg
      721 gatctggatc gtatccgtac tcgcgactcg aacgctcgcc cccgatgatc ccgcactctt
      781 cctgttcgag ggccggcctc cgcgccccct gtacctcgcc gcgagcctga tcgaggggat
      841 cgcgttccgc acatgggttg cggccgcagt cctcctggca ctggcgcgcc tctacggcct
      901 tcggcgtctc aggccgtagt cgttgacacc gaacatacgt tcgggtagcg tcccatcttg
      961 cgagggggaa tcacacgggt gtaagttgtg tcagacctgc ccggtagcct gcgggctcac
     1021 cgcaggagga ggtcgtggtc gtggaacgag agcggatgct cgacatcacc cttcagcaga
     1081 tcgagaagca gcacggcaag ggcgccgtca tgaagctcgg cgagcatccg gccggccagg
     1141 ccaccgccgt gatcccgacc gggtcgctcg cgctcgacgt ggcactcggc atcggcggga
     1201 tcccgcgcgg acgggtggtc gaggtcttcg gtccggaggg ctccggcaag acgacggtct
     1261 gtctccacat catcgccgag gcgcaggcgc gcggcggcat cgcggcgttc gtcgacgccg
     1321 agcacgcgct cgaccccacg tacgcgcgca cgctcggcgt caacatcgac gagctcctcg
     1381 tctcgcagcc cgactccggc gagcaggcgc tcgagatcgg cgacatgctc gtccggtccg
     1441 gagcgctcga cctcctcgtg atcgactcgg tggcggcgct cgtgccgcgc gccgagatcg
     1501 agggcgagat gggagacgcg cacgtcgggc tgcaggcgcg tctcatgtcg caggcgatgc
     1561 gcaagctctc gggctcgctt tcgcgcttcg acacgaccgc gatcttcatc aatcagctgc
     1621 gcgagaagat cgggatcatg ttcggcaacc cggagaccac gccgggaggc cgggcgttga
     1681 agttctactc gagcgtgcgg ctcgatgtcc gcaagatcga caacctcaag gacgggaccg
     1741 acgtcgtcgg ctcgcgcgtc cggaccaagg tcgtgaagaa caagctggcg ccgccgttcc
     1801 gtcaggcgga gttcgacatc gtctacggca aggggatctc gaaggagggg agcctcctcg
     1861 acgtcggcgt cgacaccgag atcgtgcgca agagcggcgc gtggttcacg tacgagggcg
     1921 agcagctcgg ccaggggcgc gagaacgccc gccagttcct gcaggagcac aaggagatcg
     1981 ccgcggagat cgagcggcgg gtccgagagg cggtcgggct gagcgcgttc ggcgacgccg
     2041 acgagccggt cccagacgtg gaggcggcag acgacaagga gcgagcgaag gcggcgaccg
     2101 gctagcgcgg ccgacgaccg gccgcggctc ccggtcaagg agcgtgcgct ccgtctcctc
     2161 ggggtccgct cgaggagccg agaggagctc cgccgccggc tcctcctcgc cggctaccac
     2221 ccgtcggaga tcgaatcggc gctggacgac ctcgagggag tggggctcgt cgacgacgag
     2281 cggttcgcgt cggacctcgc ttcgtacgag atgggccgac gcgggaaggg gcggcgcggc
     2341 gcggtggcgt cgctccgacg ccacggggtc
//
LOCUS       DASZOB010000318         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_116974, whole genome shotgun sequence.
ACCESSION   DASZOB010000318 DASZOB010000000
VERSION     DASZOB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116974"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..959)
                     /gene="deoC"
                     /locus_tag="VG709_05150"
     CDS             complement(75..959)
                     /gene="deoC"
                     /locus_tag="VG709_05150"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWC32497.1"
                     /translation="MVRVTPVDAVALEERAASLATRSIKRDAKLFALDLAVRCCDLTT
                     LEGSDTPGKVRALCAKARRPDPTDPTIPSVAAVVIYPHLVPVAARALDGSGVKVASVA
                     GAFPSGLAPLETRLDEIRRAVSAGADEIDIVLNRSAFLAGRYAQAYEEIAASKEACGD
                     AHLKVILETGELGSYDQVRRASVLSMAAGADFIKTSTGKIPVGATFPTALCMLEAVRE
                     FHRQTGRVVGVKVAGGIRKAKEAWTYLVIVHETLGPEWLTPDRFRIGASTLLNDLLAQ
                     IRKERSGRYQGPDYFTID"
     gene            1131..1532
                     /locus_tag="VG709_05155"
     CDS             1131..1532
                     /locus_tag="VG709_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWC32498.1"
                     /translation="MKPFALAIATAFLLVACGGDGGGGDATNGGNGGDGTATGCIDVR
                     RQVSPVVVAVDNEFDPSCLQVAEGQNLTVRNEGNAAHTFTIDEAEINVVVQPGEESVV
                     DSVGDEVPVGGEVEFRCEFHPEMTGTLRRPS"
BASE COUNT          227 a          541 c          567 g          231 t
ORIGIN      
        1 ctcccgcgcc ggcgcgtacg agaacggggt gcggtcctcc agggcccgtt gctcgcgtcg
       61 ctcgaggtct cccatcagtc gatcgtgaag tagtcggggc cctggtagcg gccgctgcgc
      121 tccttgcgga tctgcgcgag caggtcgttg aggagcgtcg acgcgccgat cctgaagcgg
      181 tcgggcgtga gccactcggg cccgagcgtc tcgtggacga tcacgaggta cgtccacgcc
      241 tccttcgctt tgcggatccc gcctgcgacc ttcacgccga cgacgcgtcc ggtctgccgg
      301 tggaactcgc ggacggcctc gagcatgcag agcgccgtcg ggaacgtcgc gccgaccggg
      361 atcttcccgg tcgaggtctt gatgaagtcg gcccccgccg ccatcgacag cacgctcgcg
      421 cggcggacct ggtcgtacga cccgagctcg ccggtctcga ggatcacctt gaggtgcgcg
      481 tcgccgcacg cctccttcga cgccgcgatc tcctcgtagg cctgcgcgta ccggccggcg
      541 aggaaggcgg agcggttcag cacgatgtcg atctcgtcgg ctccggccga gacggcgcgg
      601 cggatctcgt cgagccgggt ctcgagtggc gccaggccgc tcgggaacgc gcccgcgacg
      661 ctcgcgacct tgacgcccga tccgtcgagg gcgcgcgcgg cgaccgggac gagatggggg
      721 tagatcacga cggcggcgac ggacgggatc gtcggatccg tcggatcggg ccggcgcgcc
      781 ttcgcgcaga gcgcgcgtac cttgcccggc gtgtcgctcc cctcgagcgt cgtgaggtcg
      841 cagcagcgga cggcgagatc gagcgcgaac agcttcgcgt cacgcttgat cgagcgcgtc
      901 gccagcgagg ccgcgcgctc ctcgagcgcc acggcgtcga cgggagtgac gcgcaccatc
      961 gccgcctccg gcccgtcgac gctccatggg acgacgggcg ggcgtgtctg tgcgggaacg
     1021 ttcgccacgt tcggtccttc ggctcgcggg gacctccatg atacctgcgg ccagccgccc
     1081 agcccggacc tcggtgctac gctcgcccca gaacgagaag gaggacgccc atgaagccct
     1141 tcgccctggc catcgcaacc gcgttcctgc tggtcgcatg cggcggtgac ggcggtggcg
     1201 gcgacgccac caacggtgga aacggcggag acggcaccgc gaccggctgc atcgatgtca
     1261 ggaggcaggt gtcgcccgtc gtcgtcgcgg tcgacaacga gttcgacccg agctgcctcc
     1321 aggtggccga gggccagaac ctcacggtcc ggaacgaggg caacgccgcg cacacgttca
     1381 cgatcgacga ggcggagatc aacgtcgtcg ttcagccggg cgaggagtcg gtcgtagaca
     1441 gcgtcggcga cgaggttccc gtgggcggcg aggtcgagtt ccgttgcgag ttccaccccg
     1501 agatgacggg aacgctgcgg cgaccctcct agcttcggac cggaacgtgg gctcggagaa
     1561 gtcgct
//
LOCUS       DASZOB010000319         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_87475, whole genome shotgun sequence.
ACCESSION   DASZOB010000319 DASZOB010000000
VERSION     DASZOB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87475"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VG709_05160"
     CDS             complement(<1..202)
                     /locus_tag="VG709_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32499.1"
                     /translation="MHSRLPVFVVVALSLLVVGLGPAEARRNEQVAVRAEPRGIARAG
                     VDASFAESYGTFDWAVVDAADLG"
     gene            301..1860
                     /locus_tag="VG709_05165"
     CDS             301..1860
                     /locus_tag="VG709_05165"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC32500.1"
                     /translation="MGEDTYRLVIGGDLHDAASGETFESIDPSTGDAFTQVPSAAEED
                     VRRAAEAARKAFDEGPWPRMRGRERAKHLLAVADQIKKAGKGLAETEARDSGGTIRKA
                     RNADVAMAISTFRVFAEIASREEDEEPLPRQPISQNWIRREPIGVCAGITPFNFPLQM
                     AAWKIAPAIAAGNTIILKPASYTPLTSLMVGRLCLEAGLPEGVVNVLSGSGAGAGEPL
                     VTSPLVDKIALTGSTEVGSRIMSLAADSVKKVTLELGGKSASIVTDDVDIDYALEGIL
                     WGVFFHQGQVCSAGTRVFLHRRIYDELFPALVKKTEDIRVGPALDPASDNGPVVSQSQ
                     LENVERYVGIGRDEGAEVAVGGDRAIVDGHEGGYYYQPTILAGENGMTVAQEEIFGPV
                     LVVIPYDDDDEAIRLANDSIYGLAGAVWSRDVPRAVKIAESLRTGTVWINDYHMINPR
                     YPFGGYKRSGIGREHGTLGYNEYREVKHIHVDLAGPREKHPWWDTLLPREGDTGEAAR
                     PQAEKGRGPAG"
     gene            2086..>2536
                     /locus_tag="VG709_05170"
     CDS             2086..>2536
                     /locus_tag="VG709_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWC32501.1"
                     /translation="MPAIRCSTVVTCALPGVPARGRIEPEVVRDGVIAWEDGVVVYAG
                     PDAAWDGDIDEDVGARAVVPGFVDCHTHLPFFGWRDDEYEARLRGATYRDLHGEGGVP
                     RSARMLADASDDDVLSFSRELLVEMLEHGTTTVELKTGYGLSVGAELR"
BASE COUNT          417 a          882 c          880 g          357 t
ORIGIN      
        1 cccccaggtc cgcagcgtcg acgaccgccc agtcgaacgt gccgtacgac tcggcgaagc
       61 tcgcatcgac cccggcccgg gcgatccctc ggggctcggc ccggaccgct acctgctcgt
      121 tgcggcgggc ctctgccgga ccgagtccta cgacgaggag cgaaagggcg acgacgacga
      181 agacgggcaa gcggctgtgc atggactccc tccagacgtg gacgtatcgc ccattccatt
      241 cgccgccgcg cgccgcatcc ctgcctcgat agactcgggc tatccaggtg ggaggtcgcg
      301 atgggtgaag acacgtatcg gctcgtgatc ggcggcgatc ttcacgacgc cgcgtccgga
      361 gagacgttcg agagcatcga cccctcgacc ggcgacgcgt tcacgcaggt cccgagcgcg
      421 gccgaggagg acgtccgccg cgccgcggag gccgcgcgga aggcattcga cgagggcccg
      481 tggccgcgga tgcgcggacg ggagcgagcg aagcacctgc tcgcggtcgc cgaccagatc
      541 aagaaggccg gcaagggtct cgccgagacc gaggcgcgcg actccggcgg aacgatccgc
      601 aaggcccgca acgccgacgt cgcgatggcg atctcgacgt tccgcgtctt cgcggagatc
      661 gcctcgcgcg aggaggacga ggagccgctc ccccgtcagc cgatctcgca gaactggatc
      721 cgccgtgagc cgatcggcgt ctgcgccggc atcacgccgt tcaacttccc gctccagatg
      781 gccgcgtgga agatcgcgcc ggcgatcgcc gccgggaaca ccatcatcct caagccggcg
      841 tcgtacacgc cgctcacgtc gctcatggtc ggacgcctct gcctcgaagc gggtcttccc
      901 gaaggcgtcg tgaacgtgct ctccggctcg ggcgcgggcg ccggcgaacc actcgtcacc
      961 tcgccgctcg tcgacaagat cgcgctcacg ggctcgaccg aggtcggaag ccggatcatg
     1021 agcctcgccg cagacagcgt gaagaaggtg acgctcgagc tgggggggaa atcggcctcg
     1081 atcgtgaccg acgacgtcga catcgactac gcgctcgagg ggatcctgtg gggcgtcttc
     1141 ttccaccagg gacaggtgtg ctcggccggc acgcgcgtgt tcctccaccg caggatctac
     1201 gatgagctgt tccctgcgct cgtcaagaag accgaggaca tccgcgtcgg gccggcgctc
     1261 gacccggcgt cggacaacgg ccccgtggta tcgcagagcc agctcgagaa cgtcgagcgt
     1321 tacgtcggca tcgggcgcga cgagggcgcc gaggtcgccg tcggcggcga ccgcgcgatc
     1381 gtcgacggac acgagggcgg ctactactac cagccgacca tcctcgccgg cgagaacggg
     1441 atgacggtcg cgcaggagga gatcttcggg ccggtcctcg tggtgatccc gtacgacgac
     1501 gacgacgagg cgatccggct cgcgaacgac tcgatctatg gcctcgccgg cgcggtgtgg
     1561 tcccgcgacg tgccgcgtgc cgtgaagatc gcggagtcgc tgcggaccgg gaccgtatgg
     1621 atcaacgact accacatgat caacccgcgc tacccgttcg gcggctacaa gcgctccggg
     1681 attgggcgcg agcacggcac gctcggctac aacgagtacc gcgaggtgaa gcacatccac
     1741 gtcgacctcg cagggccgcg cgagaagcac ccttggtggg acacgctgct gccgcgcgag
     1801 ggcgacacag gcgaagcggc aaggccccag gccgagaagg gacgcggtcc ggccggctga
     1861 tcggtccggc ggcttcgtga gcgaccccag aaaagccgag agggggactg cccgcgaatc
     1921 cccctcttgg catatgcagc acccgagcgc cctctcgcgg tgctgcttcc cgtcggcgtt
     1981 caccgacagg tctattgagt tgtcgtatcg catgatttcc catcgccccc cggatcggta
     2041 tgccctgaac ggctcattcc ggatggccag aaggaactag tcaccgtgcc ggcgatccga
     2101 tgctcgacgg tcgtgacgtg cgcgctgccc ggcgtgcccg cgcgcggccg catcgagccg
     2161 gaggtcgtcc gcgacggcgt catcgcctgg gaggacggag tcgtggtcta cgcgggaccc
     2221 gacgcggcgt gggacgggga catcgacgag gacgtcggcg cgcgtgccgt cgtccccgga
     2281 ttcgtcgact gccacaccca cctcccgttc ttcggatggc gcgacgacga gtacgaggct
     2341 cggctccggg gcgcgacgta ccgcgacctc cacggcgaag gcggcgtccc ccgcagcgcc
     2401 cgcatgctcg ccgacgcgtc cgacgacgac gtcctgtcgt tctcgcgcga gctcctcgtc
     2461 gagatgctgg agcacgggac tacgacggtc gagctcaaga ccgggtacgg cctgtcggtg
     2521 ggcgccgagc tgcgcc
//
LOCUS       DASZOB010000320         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_67739, whole genome shotgun sequence.
ACCESSION   DASZOB010000320 DASZOB010000000
VERSION     DASZOB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67739"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VG709_05175"
     CDS             complement(<1..551)
                     /locus_tag="VG709_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWC32502.1"
                     /translation="MSGAVVSFFTRTAPELVERVELYQDEDPLFERYDIEKAIQSTLS
                     RRVDLPSGGYLIIDYAEAMTVIDINSGSFTGRGKSARLEDTITRTNLEAAAEVVRQLR
                     LRDIGGIIVIDFIDMSRARNRDAVLKTLRKALDEDRTKTYVVEISPLGLVEMTRQNVT
                     DGVREILTKTCPTCHGEGVVLSE"
     gene            523..>1669
                     /locus_tag="VG709_05180"
     CDS             523..>1669
                     /locus_tag="VG709_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWC32503.1"
                     /translation="MKKETTAPDMVFQEADLSVRVVRDVFAREFEKAIVDDRKQHHRL
                     ESFFTRTAPDLLERLEFYGDEQPLFESYGVEEAIQSTLRRRVDLPSGGYLMIDYAEAM
                     TVIDVNSGSFTGRGKGARLEDTITKTNLEAAEEVHRQLRLRDIGGIIVIAFIDMARAR
                     NREAVLKTLRKALDQDRTKTYVVEVSPLGLVEMTRQNVTDGVREILTKDCPTCDGEGV
                     VLSEETVAIDVERRLRELAGERPAPEAFLVRVHPKVATTLVGGPGEEGPIADVEGQTG
                     KHFHFEGTEALPIDHFEAVLEGSRAEVEERALPFREGEEVLVRIEEPHMYNEDDAVAK
                     LDGYVISVAGAGSLIGEKRLVRIDKVGRSAASASLLEADGTPPRAEAE"
BASE COUNT          268 a          535 c          614 g          252 t
ORIGIN      
        1 tcctcggaca gcacgacgcc ctcgccgtga caggtggggc acgtcttggt gaggatttcg
       61 cggacgccgt cggtgacgtt ctggcgcgtc atctcgacga ggcccagcgg cgagatctcg
      121 acgacgtagg tcttcgtccg gtcctcgtcg agcgccttac gcagcgtctt gaggacggcg
      181 tcgcggttgc gcgcgcgcga catgtcgatg aagtcgatga cgattatccc gccgatgtcc
      241 cgcagccgga gctggcgcac gacctcggcc gcggcctcca ggttggtccg tgtgatcgtg
      301 tcctcgaggc gggcgctctt gccgcggccg gtgaacgagc cggagttgat gtcgatcacc
      361 gtcatcgcct cggcgtagtc gatgatcagg tagccgcccg acgggaggtc gacgcggcgg
      421 ctgagcgtgg actggatcgc cttctcgatg tcgtagcgct cgaagagcgg atcctcgtcc
      481 tggtacagct cgacgcgctc cacgagctcc ggtgcagtgc gggtgaagaa ggagacgacc
      541 gcgccggaca tggtcttcca ggaggccgac ctgtcggtgc gggtcgtccg cgacgtgttc
      601 gccagggagt tcgagaaggc gatcgtcgac gaccgaaagc agcaccaccg gctcgagtcg
      661 ttcttcaccc gcaccgcccc ggacctgctc gagcggctcg agttctacgg ggacgagcag
      721 ccgctcttcg agagttacgg cgtggaggag gcgatccagt cgacgctcag gcggcgggtg
      781 gacctgccct ccggcggcta cctgatgatc gactacgccg aggccatgac cgtgatcgac
      841 gtgaactcgg gctcgttcac cggccgcggc aagggcgcac ggctcgagga cacgatcacg
      901 aagacgaacc tcgaggcggc ggaggaggtg caccgccagc tacggcttcg cgacatcggc
      961 gggatcatcg tcatcgcctt catcgacatg gcgcgcgcgc gcaaccgcga ggccgtgttg
     1021 aagacgctcc gcaaggcgct cgaccaggac cgcaccaaga cctacgtggt ggaggtctcg
     1081 ccgctcgggc tcgtggagat gacgcgccag aacgtgaccg acggggtgcg cgagatcctc
     1141 acgaaggact gccccacctg cgacggcgag ggggtggtgc tctccgagga gaccgtcgcg
     1201 atcgacgtgg agcggaggtt gcgcgaactg gctggcgagc ggccggcgcc ggaggcattc
     1261 ctggtccgcg tccacccgaa ggtcgcgacc acgctcgtcg gtggccccgg cgaggagggg
     1321 ccgatcgccg acgtcgaggg ccagaccggc aagcacttcc acttcgaggg cacggaggcg
     1381 ctcccgatcg accacttcga ggcggtcctc gaggggagcc gggccgaggt ggaggagcgg
     1441 gcgctgccgt tccgtgaggg cgaggaggtg ctggtcagga tcgaggagcc ccacatgtac
     1501 aacgaggacg acgccgtggc gaagctcgac ggctacgtca tctcggtcgc cggcgccggc
     1561 tcgctcatcg gggagaagcg gctcgtgcgg atcgacaaag tcggtcgctc ggcggcctcc
     1621 gcgtcgctgc tcgaggcgga cggcacgccg ccccgcgccg aggcggagg
//
LOCUS       DASZOB010000321         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_13245, whole genome shotgun sequence.
ACCESSION   DASZOB010000321 DASZOB010000000
VERSION     DASZOB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13245"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="VG709_05185"
     CDS             <1..1723
                     /locus_tag="VG709_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWC32504.1"
                     /translation="EEAVSRVEPDQLDQLLHPQFDPKGSLDVLAKGINASPGAAVGKA
                     VFDADTAQAMGEKGEDVILVRRETNPDDLHGMIAARGILTSHGGKTSHAAVVARGMGK
                     PAVCGADALAIDAGKRQFSVNGTKVKQGDVISIDGTTGRVVRGAVSLVEPKLSGDFET
                     LLSWADDLRQLKVRANADDGPSAAKARKFGAEGIGLCRTEHMFGTPGDPRLPTFRQVI
                     LTAPAARAAQLEVLEIEKEAADAKGKAKKEADKRVKEARDRLKSAEIKPYFDGLKTLL
                     AGQRKDFEAIFRAMDGLPVTIRLLDPPLHEFLPSSKELELALAAPGEKRSKDLLKEVG
                     GKRKAEAVLRDVAALEENNPMLGLRGCRLGILYPDIYRVQVQAIVEAACNVKGRGGDP
                     QVEIMVPLVSNREELETVARDVREVAEAVLERKGVDLPYLVGTMIELPRACVAADEIA
                     EEAEFFSFGTNDLTQTTFGFSRDDVEGKFMARYLDQKVLARNPFESIDEHGVGELVRT
                     AVERGRKTREDIKLGICGEHGGDPESVDFFHRVGLDYVSASPYRVPIARLAAAHAALG
                     ERDEASK"
BASE COUNT          293 a          613 c          660 g          236 t
ORIGIN      
        1 cgaggaggcc gtgtcgcgcg tcgagcccga ccagctcgac cagctgctgc acccgcagtt
       61 cgacccgaag gggtcgctcg acgtgctcgc gaaggggatc aacgcgagcc cgggcgcggc
      121 cgtcggcaag gcggtgttcg acgccgacac cgcgcaggcg atgggcgaga agggcgagga
      181 tgtgatcctc gtccgccgcg agacgaaccc cgacgacctc cacggcatga tcgccgcccg
      241 cgggatcctc acgtcgcacg gcggcaagac gtcgcacgca gcagtcgtcg cacgcgggat
      301 gggcaagccg gcggtgtgcg gcgccgacgc gctcgcgatc gacgcgggga agcgccagtt
      361 ctccgtgaac ggaacgaagg tcaagcaggg cgacgtcatc tcgatcgacg gcacgaccgg
      421 ccgcgtggtg cgcggcgcgg tctcgctcgt cgagccgaag ctctccggcg acttcgagac
      481 gctgctgtcg tgggccgacg acctccgcca gctcaaggtc cgcgcgaacg ccgacgacgg
      541 accgtcggct gcgaaggcgc ggaagttcgg cgccgagggg atcgggctct gccgtacgga
      601 gcacatgttc ggcacgcccg gcgacccgcg gcttccgacg ttccgccagg tgatcctcac
      661 ggcgccggca gcccgagccg cgcagctcga ggtgctcgag atcgagaagg aagccgccga
      721 cgcgaagggg aaggcgaaga aggaggccga caagcgggtg aaggaggcgc gcgaccgcct
      781 gaagtcggcc gagatcaagc cgtacttcga cggcctcaag acgctcctcg cggggcagcg
      841 caaggacttc gaggcgatct tccgcgcgat ggacgggctt ccggtcacga tccggctcct
      901 cgacccgccg ctgcacgagt tcctgccgtc gtcgaaggag ctcgagctcg cgctcgcggc
      961 gccgggcgag aagcgctcga aggacctcct caaggaggtc ggcggcaagc ggaaggccga
     1021 ggcggtcctc cgcgacgtcg ccgcgctcga ggagaacaac ccgatgctcg ggctgcgggg
     1081 gtgccgcctc gggatcctct accccgacat ctaccgggtg caggtccagg cgatcgtcga
     1141 ggccgcgtgc aacgtcaagg gccgcggagg cgatccgcag gtcgagatca tggtgccgct
     1201 cgtctcgaac cgcgaggagc tcgagaccgt cgcgcgcgac gtccgggagg tcgccgaggc
     1261 ggtcctggag cgcaagggcg tcgacctgcc gtacctcgtc ggcacgatga tcgagctgcc
     1321 gcgggcgtgc gtcgccgccg acgagatcgc cgaggaggcg gagttcttct cgttcggcac
     1381 caacgacctc acccagacga cgttcggctt ctcccgcgac gacgtcgagg ggaagttcat
     1441 ggcgcgctac ctcgaccaga aggttctcgc gcggaacccg ttcgagtcga tcgacgagca
     1501 cggcgtcggc gagctcgtgc ggacggcggt cgagcgcggg cggaagacgc gcgaggacat
     1561 caagctcggg atctgcgggg aacacggcgg cgacccggag tccgtcgact tcttccaccg
     1621 cgtcgggctc gactacgtct cggcctcgcc gtaccgcgtg ccgatcgcgc ggctcgcggc
     1681 ggcccacgcc gcgctcggcg agcgcgacga ggcctcgaag taacggttag cgcttctcga
     1741 actgctcctt ccagttcggg tccgtgacgg gcttccacag gagcgtcggc ggcggctcgc
     1801 ct
//
LOCUS       DASZOB010000322         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148001, whole genome shotgun sequence.
ACCESSION   DASZOB010000322 DASZOB010000000
VERSION     DASZOB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148001"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VG709_05190"
     CDS             complement(<1..268)
                     /locus_tag="VG709_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWC32505.1"
                     /translation="MNGEVVTLTIDGKAVTVPAGTLIIRAAEALGIEIPRFCEHPKLS
                     PVGACRQCIVEVEGQRKPVASCTVTVTDGMVVKTQLTSKVADKAQ"
     gene            complement(265..1611)
                     /gene="nuoF"
                     /locus_tag="VG709_05195"
     CDS             complement(265..1611)
                     /gene="nuoF"
                     /locus_tag="VG709_05195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWC32506.1"
                     /translation="MAEFERVLTARWEISGADSIDGFLDAGGYEGLRKALGMSPAEVI
                     DEVKASGLRGRGGAGFPTGVKWSFIPQDTGRPVYVVCNADEGEPGTFKDRELLERDPH
                     QLIEGIAIASYAVGCRTAFVYMRGEFLEPARKLERALAEAYDRGFLGNGIAGSDFDLD
                     IVMHRGAGSYECGEETALLDSLEGRRGQPRLRPPFPAVQGLYRSPTVVNNVETLSTVP
                     HVLARGADWFTKMGPEKSTGPKIFCVSGKVARPGNYEAPMGTPARVIIDELAGGVTDG
                     RSVKAWTPGGSSTPFLTGDHLDTPMDFESIAAAGSLLGTGAVIVMDDRDCVVDAVLRF
                     TQFYAHESCGKCTPCREGTWWLTKVLYRLENGGGRLDDIDLMYDVGDNMLFKNFCALG
                     DGAVSPIHSSIKYFRDEYVEHVKRQGCPFRDRPVVSAVAPREAGTERAIALSEVLP"
     gene            complement(1604..>2200)
                     /locus_tag="VG709_05200"
     CDS             complement(1604..>2200)
                     /locus_tag="VG709_05200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWC32507.1"
                     /translation="RRSGDGGGGPLVPVLSEDVRRRADELVARYPEPRSALLPLLYLV
                     QAEQGYVTREGMQEVGEILGLQTAEVEAVATYYTMFKMRPTGRWLVSVCTNLSCSLAG
                     ARRVYERAREHLGEAAEEVTDDEAFTLEEVECLGACDAAPVVQVNYVSYDRVDEDGIV
                     SILDALHAGDVPEPPRGPKPVDHAATSRILANLGDERG"
BASE COUNT          314 a          807 c          742 g          337 t
ORIGIN      
        1 gctgcgcctt gtccgcgacc ttcgacgtga gctgggtctt gaccaccatg ccgtcggtca
       61 ccgtgaccgt gcacgaggcc accggcttgc gctggccctc gacctcgacg atgcactgcc
      121 ggcacgcgcc gaccggggag agcttcgggt gctcacagaa gcgcgggatc tcgatcccca
      181 gcgcctcggc cgcgcggatg atgagcgtcc cggccgggac cgtgacggcc ttgccgtcga
      241 tcgtgagcgt gacgacctcg ccgttcacgg cagcacctcg gagagcgcga tcgcgcgctc
      301 ggtgcccgcc tcgcgcgggg cgacagcgct caccaccggc cggtcgcgga acgggcaccc
      361 ctggcgcttc acgtgttcga cgtactcgtc gcggaagtac ttgatcgagg agtggatcgg
      421 ggagacggcg ccgtcgccga gcgcgcagaa gttcttgaac agcatgttgt cgccgacgtc
      481 gtacatgaga tcgatatcgt cgagccggcc gccgccgttc tcgagccggt acagcacctt
      541 cgtcagccac cacgtcccct cgcggcacgg cgtgcacttc ccgcaggact cgtgcgcgta
      601 gaactgcgtg aagcgcagca cggcgtcgac gacgcagtcg cggtcgtcca tcacgatgac
      661 cgcgccggtc ccgagcagcg agcccgcggc ggcgatcgac tcgaagtcca tcggcgtgtc
      721 gaggtggtcg cccgtgagga acggcgtcga tgagcctcca ggcgtccacg ccttgacgct
      781 ccgtccgtcg gtgacgccgc cggcgagctc gtcgatgatc acgcgtgccg gcgtccccat
      841 cggcgcctcg tagttcccgg gccgtgcgac ctttcccgag acgcagaaga tcttcggccc
      901 cgtcgacttc tccgggccca tcttcgtgaa ccagtcggcg cctcgcgcga gcacgtgcgg
      961 caccgtggac agcgtctcga cgttgttaac gacggtcggg ctgcggtaca gaccctggac
     1021 cgccgggaac ggcgggcgca ggcgcggctg ccctcgccgc ccctcgagcg agtcgaggag
     1081 cgccgtctcc tcaccgcact cgtacgagcc cgcaccccgg tgcatcacga tgtcgagatc
     1141 gaagtcgctt ccggcgatcc cgttcccgag gaagccgcgg tcgtacgcct cggcgagcgc
     1201 ccgctccagc ttgcgggccg gctcgaggaa ctcgccgcgc atgtagacga acgcggtgcg
     1261 gcatccgacc gcgtacgacg cgatcgcgat cccttcgatc agctggtgcg gatcgcgctc
     1321 gaggagctcc cggtccttga acgtcccggg ctccccttcg tcggcgttgc agacgacgta
     1381 cacggggcgg ccggtgtcct gggggatgaa ggaccacttc acgccggtcg ggaagccggc
     1441 gccgccgcgg ccgcggagcc cggacgcctt cacctcgtcg attacctccg cgggcgacat
     1501 cccgagcgcc ttgcggagtc cctcgtatcc gccggcgtcg aggaacccgt cgatcgagtc
     1561 ggcgccgctt atctcccaac gcgccgtcag cacgcgctcg aactcagcca cgctcgtctc
     1621 cgaggttcgc gaggatgcgt gaggttgcgg cgtggtcgac gggcttcggg cctcggggcg
     1681 gctccggcac gtcgcccgcg tggagcgcgt cgaggatcga cacgatcccg tcctcgtcga
     1741 cccggtcgta gctgacgtag ttcacctgga cgacgggcgc ggcatcgcac gcgccgaggc
     1801 actcgacctc ctcgagcgtg aacgcctcgt cgtccgtcac ctcctcggcc gcctcgccga
     1861 ggtgctcgcg cgcgcgctcg tacacccgcc gcgcccccgc gagcgagcat gagaggttcg
     1921 tgcacaccga gacgagccag cgccccgtcg gccgcatctt gaacatcgtg tagtaggtcg
     1981 cgacggcctc gacctcggcc gtctggagcc cgaggatctc gccgacctcc tgcatgccct
     2041 cgcgggtgac gtatccctgc tcggcctgga cgaggtagag cagcggcagg agcgccgacc
     2101 gcggctccgg gtagcgcgcg acgagctcgt cggcacggcg gcggacgtcc tcggagagca
     2161 ccggcactag cggtccaccc cccccatcac cggatcgacg
//
LOCUS       DASZOB010000323         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_43711, whole genome shotgun sequence.
ACCESSION   DASZOB010000323 DASZOB010000000
VERSION     DASZOB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43711"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VG709_05205"
     CDS             complement(<1..302)
                     /locus_tag="VG709_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWC32508.1"
                     /translation="MKTTVAELPENKGRLEGEVPEADVKLALDHAASDLAATARIPGF
                     RKGKVPLRLIVSRLGRDAVWQEAVRSHLEGWFWSAAASSGIQPVGSPELERGEEPG"
     gene            413..1465
                     /locus_tag="VG709_05210"
     CDS             413..1465
                     /locus_tag="VG709_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32509.1"
                     /translation="MRTLVTLLDYANLALFTLVALAALREWRAGRGRAGLWAALTFSA
                     LAVVVTVGRVVPEDPMNAVEFVVSKALVGLLVLFPYFLYRFTAAFQASTPRVAHVLGV
                     MTMVLLATTFVTPEFPQAGEPRPAWFLAYLIAFLVHWTVLASAVAVRLWRAGSGEPSV
                     ARRRMRLLGLASIAITTALLLAAGGPREASALDLAATLLAMVSAVAFLLGLAPPQVLR
                     MLWRRPEQQALQASIAKLMGATTQEEVVREVLPPMARIVGARAIALRDANGRTIATHG
                     VSEEMVTASLEDNPGEDEGGAEDVLRLDVPSGCLVIWRGPYAPFFGSDELALLRTLGA
                     LTGLALDRSRLFADER"
BASE COUNT          192 a          618 c          562 g          252 t
ORIGIN      
        1 tccccgggct cctcgccgcg ctcgagctcc ggcgacccga ccggctggat gccggacgag
       61 gccgcggcgc tccagaacca gccctcgagg tggcttcgca ccgcctcctg ccacacggcg
      121 tctcgtccca gacgcgagac gatcagtcgc aacgggacct tgcccttgcg aaagcccggg
      181 atccgggccg tcgcggcgag gtcggaggcg gcgtggtcga gcgcgagctt gacgtcggcc
      241 tccggcacct cgccctcgag gcgccccttg ttctcgggca gctctgcgac cgtcgtcttc
      301 accgccgggc cattgtgcca ccgcgccggc cggccccgcg ccggaggcac gccgtgaggc
      361 tgcggcccgc ggtgactcgt acagttcggg aaaccacccg tcgccattcg ccatgcgcac
      421 gctcgtcacc cttctcgact acgcgaacct cgcgctgttc acgctggtgg cgctcgcagc
      481 gcttcgcgag tggcgggcgg ggcgcggccg cgccggcctg tgggcggcgc tgacgttcag
      541 cgcgctggcg gtcgtggtca ccgtcggccg cgtcgttccc gaagacccga tgaacgccgt
      601 cgagttcgtc gtgtcgaagg cgctcgtcgg cctgctcgtc ctcttcccct acttcctcta
      661 ccggttcacc gcggccttcc aggcgtcgac gccgcgcgtc gcgcacgtcc tcggcgtcat
      721 gacaatggtc ctcctcgcca cgacgttcgt gacgcccgag ttccctcagg ccggcgagcc
      781 gcggccggcg tggttcctcg cttacctcat cgcgttcctc gtgcactgga cggtgctcgc
      841 ctcggccgtc gcggtgcggc tctggcgcgc ggggagcggc gagccgagcg tcgcgcgacg
      901 ccggatgcgg ttgctcgggc tcgcctcgat cgcgatcacg accgcgctcc tgctcgccgc
      961 gggcggaccc cgcgaggcga gcgcgctcga cctcgccgcg acgctgctcg cgatggtgag
     1021 cgcagttgcg ttcctcctcg gcctcgcgcc gccgcaggtt ctccgtatgc tctggcgccg
     1081 acccgagcag caggcgctcc aggcgagcat cgccaagctg atgggtgcca cgacccagga
     1141 ggaggtcgtg cgcgaggtgc tcccgccgat ggcgcgcatc gtcggtgcgc gggcgatcgc
     1201 gctccgcgac gcgaacgggc gcacgatcgc gacacacggc gtttcggaag agatggtcac
     1261 ggcgtccctc gaggacaacc cgggcgagga cgagggcggt gcagaggacg ttctccgcct
     1321 cgacgtcccg tcgggatgcc tcgtcatctg gcgaggcccg tatgcgccgt tcttcgggag
     1381 cgacgagctc gcccttctgc ggacgctcgg cgcgctgacc gggctcgcac tcgaccgctc
     1441 ccggctcttc gcggacgagc ggtagacacg gctcgcgctg gagcgggccg acgaggtgaa
     1501 gacgaacttc gtcgcgctcg ccgcgcatga gctgcggacg ccggttgcca cgatcgacgg
     1561 gatcatccag acgctccgcg cccgcggcgc ccgcctcccc caggaccacc gccgcctcct
     1621 cgag
//
LOCUS       DASZOB010000324         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_43741, whole genome shotgun sequence.
ACCESSION   DASZOB010000324 DASZOB010000000
VERSION     DASZOB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43741"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VG709_05215"
     CDS             <1..614
                     /locus_tag="VG709_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HWC32510.1"
                     /translation="ITGELGKIRHPEVRVNVIHTGIGGISESDVMLASASNAVVVGFN
                     VRPNADARLLAEREGVDLRTYRVIYQLTDDIEQALVGMLSPERVEETLGEAEVRALFR
                     ASRIGVIAGCYVTNGVIRRNARMRVVREGTVVHETTIASLKRFKDDVREVQAGFECGI
                     LLDGFNAVKEGDVLEAFETREIERTELSTGNGGPPADEAPEEE"
     gene            860..1159
                     /locus_tag="VG709_05220"
     CDS             860..1159
                     /locus_tag="VG709_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWC32511.1"
                     /translation="MAGGYVGILRVELHFPTNHSLKGKRKELLSTKAHLQRRFGAAVA
                     EVDHHDVWQRAGLAVACVAREYREVERLLDEAERYLDGQSYVLSRSEREVVSVDE"
     gene            1164..1505
                     /gene="rbfA"
                     /locus_tag="VG709_05225"
     CDS             1164..1505
                     /gene="rbfA"
                     /locus_tag="VG709_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWC32512.1"
                     /translation="MRRVNEALREVLSESVGELKDPRIGFVTVTGVQTSPDLRHATVY
                     VSVLGSERKRDASLRGLAHARGILQRRVNQELHLKRTPQLSFEYDPTVERGVRLAKLI
                     DELAPDDTDED"
     gene            1486..>1803
                     /locus_tag="VG709_05230"
     CDS             1486..>1803
                     /locus_tag="VG709_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWC32513.1"
                     /translation="MTQTKTDLEHVANAVRAHERFVVTTHENPDGDALGSLLGTKLLL
                     DALGKETAMYLAGTVPPPREYRFMPLEEVERGAAPPMEGRVLVAVDCANESRLGRDAG
                     RLLD"
BASE COUNT          274 a          621 c          657 g          251 t
ORIGIN      
        1 cgatcacggg cgagctgggc aagatccggc acccggaggt ccgcgtgaac gtcatccaca
       61 cggggatcgg cgggatctcc gagagcgacg tcatgctcgc ttccgcgtcg aacgcggtcg
      121 tcgtcggctt caacgtgcgc ccgaacgcgg acgcgcgttt gctcgccgag cgcgagggcg
      181 tcgacctccg gacctaccgc gtcatctacc agctcaccga cgatatcgag caggcgctcg
      241 tcggcatgct ctcgcccgag cgcgtcgagg aaacgctcgg cgaggcggag gtgcgcgcgc
      301 tcttccgcgc ctcgcgcatc ggcgtcatcg cggggtgcta cgtcacgaac ggcgtcatcc
      361 gccgcaacgc gcgcatgcgc gtcgtccgcg agggaaccgt cgtccacgag acgacgatcg
      421 cgagcctcaa gcgcttcaag gacgacgtcc gcgaggtgca ggcgggcttc gagtgcggga
      481 tcctcctcga cggcttcaac gccgtgaagg agggcgacgt cctcgaggcg ttcgagaccc
      541 gcgagatcga gcggaccgag ctctccaccg ggaacggcgg cccgcccgcc gacgaggcgc
      601 ccgaggagga gtgacgccag ccgggtgtcg tccgctcgtg ccggacgccg gccgggcgcg
      661 ccgatgacct gctcggccga gatggttgcc cggaagtttg cgggcgcccc tggtggccgt
      721 gacgcggcgc cggatcagca cgttcgggtg ctgcacgtag cgctgctacg ggccgcgccc
      781 ggccgcggtg cccggcttgg tcacgtccat ccccgccgga gcgcgaattt ccggccaccc
      841 atctagcggg ccgcgcgaga tggccggcgg gtacgttggc atccttaggg tcgagctgca
      901 cttcccgacg aaccactcgc tcaaggggaa gcggaaggag ctcctctcga cgaaggcaca
      961 cctccagcgc cgcttcgggg cagccgtggc cgaggtcgac caccacgacg tctggcagcg
     1021 agcgggcctc gccgtcgcgt gcgtcgcgcg cgagtaccgg gaggtggagc ggctgctgga
     1081 cgaggcggag cggtacctcg acgggcagtc gtacgtgctg tcgcgcagcg agcgcgaggt
     1141 ggtgagcgtc gatgagtgaa cgcatgcgca gggtgaacga agcgcttcgc gaggtcctct
     1201 cggagtccgt cggcgagctc aaggacccgc ggatcgggtt cgtgacggtc accggcgtcc
     1261 agacgagccc ggaccttcgc cacgcgaccg tgtacgtgag cgtcctcggc tccgagcgga
     1321 agcgcgacgc gagcctccgc ggcctcgcgc acgcgcgcgg gatcctccag cgccgcgtga
     1381 accaggagct ccatctcaag cggactccgc agctctcgtt cgagtacgat ccgacggtcg
     1441 agcggggggt ccggctcgcg aagctgatcg acgagcttgc gcccgatgac acagacgaag
     1501 actgatctcg agcacgtcgc gaacgccgtc cgggcgcacg agcgcttcgt cgtcacgacg
     1561 cacgagaatc ccgacggcga cgctctcggc tcgctgctcg ggacgaagct cctgctggac
     1621 gcgctgggca aggagacggc gatgtacctc gccggcacgg tcccgccgcc gcgtgagtac
     1681 cggttcatgc cgctcgagga ggtcgagcgt ggggcggcgc ctccgatgga gggtcgcgtg
     1741 ctcgtcgcgg tcgactgcgc gaacgagagc cggctcggcc gcgacgcggg ccgcctcctc
     1801 gac
//
LOCUS       DASZOB010000325         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148126, whole genome shotgun sequence.
ACCESSION   DASZOB010000325 DASZOB010000000
VERSION     DASZOB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148126"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1717)
                     /locus_tag="VG709_05235"
     CDS             complement(95..1717)
                     /locus_tag="VG709_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWC32514.1"
                     /translation="MATSSSPPARRGGLRAALESVFPFERFGDERTILAGTGQSGVGM
                     VVAILATFGTQVLITRVLGAPAFGVVTLATQGALVLGFFSRVGMDMAAVRQVAIDMGR
                     ERPGRVRPIVARASVIATVASLVVGGAVFAFAEPLARAFASGFDPRSDAVWAFRAAAA
                     AIPGAALVQVYLGATRGLKVMRHTLYVFWVGQPLGWLALIVVGWLVARTVGITTLAYA
                     ASWLAATAWAWWLWRRETRGFGEEAPAPSETGELLRYGAPRAPAALFSQLLFWTDLFV
                     LAQFAGRFGVTQTDVGIYAAAARAAQVILLFIIATSLMFSPFVADLHARGQREKLDRL
                     FKQLTRWTMAATLPLFIVLAVTPGSALRLFGDEFGAGQIALLILLLGQLVNVATGSVG
                     FVLIMVGRTGWDLVVYAGSVALDIGVAVALIPVLGMEGAAIAGALTMAVSKVARLALV
                     WRFVRIQPFDRDYARLVVPGGAGLVAGLAAHLAVSGAGWPVDLLVTGAAAGLVYAVVL
                     VAAGLPPVERRALGRLVGALRGGTHPSADSGR"
     gene            complement(1701..3281)
                     /locus_tag="VG709_05240"
     CDS             complement(1701..3281)
                     /locus_tag="VG709_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC32515.1"
                     /translation="MSRVLLIGWDGADWRILDPMIEEGILPNLAALVNRGAKGVLRST
                     TPNHSWAAWPSFLTGVEPSNHGVYDIFERNLGSRKQLPVTYHSIKEKTMLADLAAAGT
                     EVVMTNLPLTFPPPKIRGKLVAGGVLPKWRPYTHPESLASELDAAGAPFPINGMSWTT
                     FRNRPDAFLDEATELTKARTKANLHLLDTTDWRFAALIYVATDRIQHCLAKYISPDGP
                     DYATLSRDRIAGRIKDVYRLLDDGLGQILERTRDDDTILFISDHGFQTCTRALHMDRL
                     LEDLGFLRFAASQAVFGPMQWGWARAAARKLYDTLGLHGRVSLPQSVSWQKTIAYTSV
                     RSTGEGVSINVAGREPDGIVDPADFESTRDKVMEALDGFVDPKTGTKPFRSVRRKEEV
                     FTGKHAETAPDILLEPAPLYSLTHAKAMIEDADWLSGDHRIEGVIAAAGPKVRPDAFR
                     EPPLLVDMAPTILAALGVPASVKHDGQVVGTVVGDEASVDAAKAGVVDESPEADAGLD
                     ADEAQEVEEHLRGLGYLE"
     gene            3591..>4082
                     /locus_tag="VG709_05245"
     CDS             3591..>4082
                     /locus_tag="VG709_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC32516.1"
                     /translation="MSIRDLTKSFGSQVIWEDVTCDIPQSRITVMLGPSGTGKSVLLK
                     HLVVLLRPDRGEIWIDGQNLPTLNERDLYRVRRKFGVLFQDGARFGSMNVYDNVAFPL
                     REHTKKGEREIRDIVTDRLEMVGLRGAEKKLPGEISGGMKKRAGLARALVLDPEIVLF
                     DEPD"
BASE COUNT          646 a         1452 c         1452 g          532 t
ORIGIN      
        1 gaatagccga cgcccgcgac cgcgacgggg gcggcggcga ggacagcggc ggcgatcgag
       61 gccaggagga gggcgcggcg catcggcgct aagtctatcg gccgctatcg gccgaggggt
      121 gagtcccgcc gcggagcgcg ccgacgagcc gcccgagcgc gcgccgctcg accggcggaa
      181 gaccggcggc gacgaggacc accgcgtaga cgaggcccgc ggccgcgccg gtgacgagga
      241 ggtccaccgg ccagccggct ccggagaccg cgagatgggc ggcgagcccc gccacgagcc
      301 cggcgccgcc gggcaccacg aggcgggcgt agtcgcggtc gaacggctgg atcctcacga
      361 accgccagac gagcgcgagc cgcgcgacct tcgagaccgc catcgtgagg gcgccggcga
      421 tcgcggcgcc ctccatcccg aggaccggga tcagcgccac cgcgacgccg atgtcgagcg
      481 cgacggaccc cgcgtacacg acgaggtccc acccggtacg cccgaccatg atcagcacga
      541 acccgacgga gcccgtcgcg acgttcacga gctgcccgag gaggaggatc agcagcgcga
      601 tctgtcccgc gccgaactcg tcgccgaaca gacgcagcgc ggaccccggc gtcacggcga
      661 gcacgatgaa aagcggcagc gtcgccgcca tcgtccagcg cgtcagctgc ttgaagagcc
      721 ggtccagctt ctcccgctgg ccgcgcgcgt ggaggtccgc gacgaacggg ctgaacatca
      781 agctcgtcgc gatgatgaac agcaggatca cctgcgccgc tcgagcggcc gccgcgtaga
      841 tcccgacgtc ggtctgcgtg acgccgaagc gcccggcgaa ctgcgcgagc acgaacaggt
      901 ccgtccagaa aagcagctgc gagaacagcg cggcgggggc gcgcggcgct ccataccgca
      961 ggagctcgcc ggtctcgctg ggcgcgggcg cctcctctcc gaacccgcgc gtctcacgcc
     1021 gccacaacca ccacgcccac gcggtcgcgg cgagccacga ggccgcgtac gccagcgtcg
     1081 tgatgccgac cgtgcgcgcg acgagccatc cgacgacgat cagcgcgagc catcccagcg
     1141 gctgcccgac ccagaagacg tacagcgtgt gccgcatcac cttcagcccg cgcgtggcgc
     1201 cgaggtacac ctgcacgagc gccgcgccgg gaatcgccgc ggccgcggcg cggaacgccc
     1261 atacggcgtc gctccgcggg tcgaacccgg atgcgaacgc gcgggcgagc ggctcggcga
     1321 acgcgaatac ggcgccgccg acgacgaggc ttgccacggt cgcgatcact gacgcgcggg
     1381 cgacgatcgg ccgaacccga cccggccgct cgcgccccat gtcgatcgcg acctggcgca
     1441 cggcggccat gtccatcccc acccgcgaga agaacccgag gacgagcgcg ccctgggtcg
     1501 cgagcgtcac gacgccgaac gccggagcgc cgagcacgcg ggtgatgagg acctgcgtcc
     1561 cgaacgtcgc caggatggcc accaccatgc cgacaccgct ctggcccgtg ccggcgagga
     1621 tggtgcgctc gtcgccgaac cgctcgaacg ggaagacgga ctcgagggcg gcgcgcaggc
     1681 cgccccgtct ggccggcggg ctactcgagg tagccaaggc cgcggaggtg ctcctcgacc
     1741 tcctgggcct cgtcggcgtc gagcccggcg tcggcctcgg gcgactcatc gacgacgccc
     1801 gccttggcgg cgtcgaccga cgcctcgtcc ccgacgaccg tgccgacgac ttgcccgtcg
     1861 tgcttgaccg acgccggcac gccgagcgcg gcgaggatcg tcggcgccat gtcgacgagc
     1921 agcggcggct cgcggaacgc gtcggggcgc accttcgggc ccgccgcggc gatgacgccc
     1981 tcgatccggt ggtccccgga gagccaatcg gcgtcctcga tcatcgcttt cgcgtgcgtg
     2041 aggctgtaga gcggcgccgg ctcgaggagg atgtccgggg cggtctccgc gtgcttcccg
     2101 gtgaagacct cctccttgcg ccggacgctg cggaacggct tcgtgccggt cttggggtcg
     2161 acgaacccgt cgagcgcctc catgaccttg tcgcgggtcg actcgaagtc ggcggggtcg
     2221 acgatcccgt cgggctcgcg ccccgcgacg ttgatcgaga cgccctctcc cgtggaacgc
     2281 accgacgtgt acgcgatcgt cttctgccag ctcaccgact gcgggagcga gacgcggccg
     2341 tggagcccta gcgtgtcgta cagctttcgc gccgcggcgc gcgcccagcc ccactgcatc
     2401 gggccgaaca ccgcctggct cgcggcgaac cgcaggaagc cgagatcctc gaggagccgg
     2461 tccatgtgga gcgcgcgcgt gcacgtctga aagccgtggt ccgagatgaa caggatcgtg
     2521 tcgtcgtcgc gcgtccgctc caggatctga cccagcccgt cgtcgaggag ccggtagaca
     2581 tccttgatgc ggcccgcgat ccggtccctg gagagcgtcg cgtagtccgg cccatccggc
     2641 gatatgtact tcgcgaggca gtgctggatg cggtcggtcg cgacgtagat gagggcggcg
     2701 aaccgccagt cggtcgtgtc gagcaggtgc aggttcgcct tcgtccgtgc cttcgtgagc
     2761 tcggtggcct cgtcgaggaa cgcgtcgggg cggttccgga acgtcgtcca cgacatcccg
     2821 ttgatcggga acggcgcgcc cgcggcgtcg agctcggagg cgaggctctc tggatgcgtg
     2881 tacggccgcc acttcggcag cacgccgcct gcgacgagct tgccccggat cttcggcggc
     2941 gggaacgtga gcgggaggtt cgtcatgacg acctcggtcc ccgccgcggc gaggtccgcg
     3001 agcatcgtct tctccttgat cgagtgatac gtgaccggca gctgcttccg cgacccgagg
     3061 ttccgctcga agatgtcgta gacgccgtgg ttcgacggct cgacgccggt caggaacgac
     3121 ggccaggccg cccacgagtg gttcggcgtc gtggagcgga ggacgccctt cgcgccgcgg
     3181 ttcacgagcg ccgcgaggtt ggggaggatc ccctcctcga tcatcgggtc gaggatccgc
     3241 cagtccgctc cgtcccagcc gatcagaagg acgcgtgaca tggcgcgtca gtgtagcgac
     3301 tcgctcgggg cgcgaacggg cgcggggagg agatgacgcc taggcgtcga acatcgacaa
     3361 caggcccggg ccgcagaatg cccgcgtgcg gtccgggcct taccttccgg cttccgcccg
     3421 cgaccgaccg gaaggagcgg tttcggtggc atcgaactaa tattcgctct ccggccggcg
     3481 cttcgggagg cgagaggatg gcccgggtga acgtcgaagc ggttcccgcg gatgccgagg
     3541 cgtccgacgt cgagccgcgc gacccggact ccggtggcta cgaggcgtcg atctcgatca
     3601 gggacctcac gaagtccttc gggagccagg tcatctggga ggacgtcacc tgcgacatcc
     3661 cccagagccg catcaccgtg atgctcggcc cctcggggac cggcaagagc gtgctcctca
     3721 agcacctcgt cgtgctcctc cggcccgatc gcggcgagat ctggatcgac ggccagaacc
     3781 tcccgacgct gaacgagagg gacctctacc gcgtccgccg gaagttcggc gtcctcttcc
     3841 aggacggggc gcgcttcggc tcgatgaacg tctacgacaa cgtcgcgttc ccgctccgag
     3901 agcacacgaa gaagggcgag cgtgagatcc gcgacatcgt gaccgaccgg ctcgagatgg
     3961 tggggctccg cggcgccgag aagaagcttc ccggcgagat ctcgggcggg atgaagaagc
     4021 gcgccggcct cgcgcgcgcc ctcgtgctcg atccagagat agtcctcttc gacgagccag
     4081 ac
//
LOCUS       DASZOB010000326         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_27207, whole genome shotgun sequence.
ACCESSION   DASZOB010000326 DASZOB010000000
VERSION     DASZOB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27207"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VG709_05250"
     CDS             complement(<1..547)
                     /locus_tag="VG709_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWC32517.1"
                     /translation="MGVDRLNYTRVILDAVDTRAETEHMFERVVMGVDPGTAATGVAV
                     VAAKGSRRVVLSATTVRTPAGAPESDRLYALHRAVRRTLAESHPDAVAIERLLWGKNV
                     ASAMAVSRASGVVLVAAAEAGLPVREYAPPEVKMAVTGSGTAGKDDVRRALARLHAIA
                     DVPREPDAADAVAVAVCDLQQY"
     gene            complement(590..1150)
                     /locus_tag="VG709_05255"
     CDS             complement(590..1150)
                     /locus_tag="VG709_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWC32518.1"
                     /translation="MAAKLIYSSLSSLDGYVADEDGNFEWAAPDGEVHNFINDVERRI
                     GTYLYGRRMYDVMVAWESIELSDQPRFMQDYAAIWRAADKIVYSRSLEEVRSARTRVE
                     RSFDPEAVRQMKSVARGDLGVGGAEVAAQALNAGLVDEIHLFVAPVVVGGGKAALPRR
                     LSLALELVEERAFSSGFVHLSYRVRM"
     gene            complement(1192..1941)
                     /locus_tag="VG709_05260"
     CDS             complement(1192..1941)
                     /locus_tag="VG709_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWC32519.1"
                     /translation="MSGHSKWSSIKHKKAVTDQRRGKMFSKLLRAVEVAAREGGGNVE
                     SNATLASAVEKARDFSVPVDNIDRAIKRGTGELEGARYEPAVYEGYAPGGVAVLVEAM
                     TDNRNRTGQEVRHTFTKLGGNLGDPGSVAWMFDRRGVILVEASAADEDRLLEIVLDAG
                     AQDLTLEGDQWQVVTPAEALHAVREALEAAGVPIASAEPTMVPRTSVPVEGSKARAVL
                     ALIEALDDLDDVQSVNANFDIPDEVLAEVAS"
     gene            complement(1942..>2046)
                     /locus_tag="VG709_05265"
     CDS             complement(1942..>2046)
                     /locus_tag="VG709_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785013.1"
                     /note="with PdxST is involved in the biosynthesis of
                     pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of
                     glutamine to glutamate and ammonia; PdxS utilizes the
                     ammonia to synthesize pyridoxal 5'-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWC32520.1"
                     /translation="RAVVLEEGNLLAASFHPELVRDTRLHRRLLERVG"
BASE COUNT          307 a          747 c          665 g          327 t
ORIGIN      
        1 ggtattgctg caggtcgcaa acggcgaccg ctaccgcgtc ggcggcatcc ggctcgcgtg
       61 ggacgtcggc gatcgcgtgg agccgggcga gcgctcgccg gacgtcgtcc ttccccgcag
      121 tgccgcttcc ggtcacggcc atcttcacct cgggcggcgc gtactcgcgg accgggagcc
      181 cggcctcagc ggcggcgacc agaacgacgc ccgacgcgcg cgagaccgcc atggcgctcg
      241 cgacgttctt gccccagagg aggcgctcga tcgccacggc atcggggtgg ctctcggcga
      301 gcgtgcgtct caccgcgcgg tggagcgcgt acaggcgatc gctctccggc gcgccggcag
      361 gcgtccggac cgtggtcgcc gacaggacga cgcgacgcga ccccttcgcg gccacgacgg
      421 cgacacccgt cgcggcggtc cctggatcga cccccatcac gacgcgctcg aacatatgtt
      481 cagtctcggc gcgagtatcg accgcgtcaa ggatgacacg cgtgtaattt agccggtcga
      541 cgcccatgcc gcacgccgac ttccgctcat gccgatgcgg tgccgtctcc tacatccgga
      601 cccggtagct aaggtgaacg aagccgctgc tgaacgcacg ctcctcgacg agctcgagcg
      661 ccaggctgag tcgccgcggg agcgcagcct tgccgccccc caccacgacc ggggcgacga
      721 atagatggat ctcgtcgacg agccccgcgt tcagcgcctg ggccgcgacc tcggcgcctc
      781 ccacgccgag atcaccccgt gccacggatt tcatctgccg gacggcttcg gggtcgaacg
      841 atcgctcgac ccgcgtcctt gcgctacgga cctcctcgag tgatctggag tacacgatct
      901 tgtcggccgc acgccatatc gccgcgtagt cctgcatgaa gcgcggctgg tcgctgagct
      961 cgatcgattc ccaagcgacc atcacgtcgt acattcggcg tccgtagagg tacgtgccga
     1021 tccgccgctc cacgtcgttg atgaagttgt gcacctcccc gtcgggcgcc gcccattcga
     1081 agttgccatc ctcgtcggcc acatagccgt cgagcgacga gagcgacgag tagatcagct
     1141 tggccgcgat cggcctcgcc tcaccgatct ttcgagccga ggggtcacgc gctagctcgc
     1201 gacctcggcg agcacctcgt cggggatgtc gaagttggcg ttaaccgatt gaacgtcgtc
     1261 gaggtcgtcg agggcctcga tcagcgcgag gacggcacgc gccttcgagc cctcgaccgg
     1321 gacgctggtg cgagggacca tcgtcggctc ggccgacgcg atcggcacgc cagccgcctc
     1381 caaggcctcc cgcacggcgt gcagcgcttc cgccggggtg acgacctgcc actgatcgcc
     1441 ctcgagcgtc aggtcctgcg cgcctgcgtc gagcacgatc tcgaggagcc ggtcctcgtc
     1501 tgcggccgac gcctcgacga ggatcacgcc gcgtcggtcg aacatccacg cgacgctgcc
     1561 cgggtcgccg aggttgccgc caagcttcgt gaacgtgtgg cgaacttcct gaccggtgcg
     1621 gttccggttg tcggtcatcg cctcgacgag gaccgcgacg cccccgggcg cgtacccctc
     1681 gtacaccgcc ggctcgtagc gcgcgccctc gagctcgccg gtcccccgct tgatcgcccg
     1741 atcgatgttg tcgaccggga ccgagaagtc gcgggccttc tcgacggcgg aggctagcgt
     1801 tgcgttggac tcgacgttcc cgccgccctc gcgcgcggca acctcgaccg cgcgcaggag
     1861 cttcgagaac atcttcccgc gccgctgatc ggtgacggcc ttcttgtgct tgatcgacga
     1921 ccacttggaa tggccggaca actagcccac cctctccagc agccggcggt gcagacgcgt
     1981 gtcgcgaacc agctcgggat ggaaagacgc tgcgagcagg ttcccctctt cgagcacgac
     2041 cgcccg
//
LOCUS       DASZOB010000327         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148138, whole genome shotgun sequence.
ACCESSION   DASZOB010000327 DASZOB010000000
VERSION     DASZOB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148138"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..665)
                     /locus_tag="VG709_05270"
     CDS             complement(120..665)
                     /locus_tag="VG709_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32521.1"
                     /translation="MDAMTLSEWLSFAIGCVEIVLAIVVLRHVARFARTFPWLIALML
                     FFVLRGADRIYAGLTERGSLGLLVDVLVLAVLVLLLLGIEGTVGGLQAARDAAANREA
                     EYARALADYRRLARHRLANPLTVIRGGVLTLKGKPDLDAETRERLLDTIDQEALRLER
                     VALEPDAESEEEAGLRPRPRA"
     gene            683..955
                     /locus_tag="VG709_05275"
     CDS             683..955
                     /locus_tag="VG709_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32522.1"
                     /translation="MAGLDGSWRIERESGVLPPFGLSKLIAGEHGWTLVGGLPAAYFR
                     VHGHTLDYLGWPIRDELHPRKDGSWGGRGLMFGREFCRFRLVRERT"
     gene            989..>2029
                     /locus_tag="VG709_05280"
     CDS             989..>2029
                     /locus_tag="VG709_05280"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWC32523.1"
                     /translation="MRRVAITGMGAVTPVGNDAKSTWDALVAGRSGIDFIRAFDASDF
                     PVRIAGEVKDFDPSDVIAPKKARQLDRAVLFALAAAREAMDDAGLNGFKPERVGVVLG
                     SCVGGIGQAIEQWETLKERGWNRMSPYFLPNFLVDSPSGYLAMELGLKGPNCAVVSAC
                     ATGSQSIGEGADLIRSGQADAVLAGGTEGAITPLILGGFCSMRGLVHEEEDPTRASRP
                     FDATRAGFVMAEGAAVLLLEELDAAKERGATIYAEVLGHGASNDAYHMAQPDPEATGV
                     VAMMRQALAIAEVEPGRVDYINAHGTATPLGDVAETKALREVFGDHADELAVSSTKSM
                     IGHLFGAAGAVEA"
BASE COUNT          308 a          692 c          730 g          299 t
ORIGIN      
        1 cccgcggaag ccgtcccact tcggctcgta ctgccagccg ttcgccgccc ggagctcctc
       61 gacgagctcc gcctccatcg gcggaaacgg cagggatttc ggaacgggcg gcgcggcgct
      121 cacgcgcgtg ggcgcggccg aagccccgcc tcctcttccg attccgcgtc cggctcgagg
      181 gcgacccgct cgagccggag cgcctcctgg tcgatcgtgt cgagcagacg ctcgcgcgtt
      241 tcggcgtcga gatcaggctt gcccttcagc gtaaggacgc cgccgcgtat caccgtgagc
      301 gggttcgcga ggcggtggcg ggcgagccgc cggtagtcgg ccagggcgcg cgcatactcg
      361 gcctcccggt tcgcggccgc gtcgcgcgcc gcctgtagtc cgccgacggt gccttcgatc
      421 ccgagaagaa ggaggacgag gacggcgagc acgagcacgt ccacgagaag accgagcgac
      481 cctctctcgg ttagaccggc ataaattctg tccgctccgc gcagaacgaa gaacagcatg
      541 agagcgatga gccagggaaa tgtgcgcgcg aaccgcgcga cgtggcggag gacgacgatc
      601 gcgagcacga tctcgacgca gccgatcgcg aagctcagcc actcgctcag cgtcatcgcg
      661 tccacgcgag gagcctaacg cggtggcggg tctcgacggc tcctggcgga tcgagcgcga
      721 gtcgggcgtc ctcccgccct tcggcctcag caagctgatc gcgggggagc acggctggac
      781 gctcgtcggc ggcttgcccg cggcgtactt ccgcgtgcac gggcatacgc tcgactatct
      841 cggctggccc atcagagacg agctgcaccc gcggaaggat ggttcctggg gcgggcgcgg
      901 gctcatgttc ggccgcgagt tctgccgctt ccgcctcgtg cgcgaacgaa cgtaaccgct
      961 tttgcctcgc cgccgtctaa ccggcgagat gagaagagtc gcgatcacgg gcatgggcgc
     1021 cgtcacgccc gtcggcaacg acgcgaagtc gacgtgggac gcgctcgtcg cgggccggag
     1081 cgggatcgat ttcattcgcg ccttcgacgc gagcgacttt cccgttcgga tcgccggcga
     1141 ggtgaaggac ttcgacccgt cggacgtgat cgcgcccaag aaggcgcgcc agctcgaccg
     1201 cgctgttctc ttcgcgctcg ccgccgcgcg cgaggcgatg gacgacgcgg ggctcaacgg
     1261 cttcaagccg gagcgggtcg gcgtcgttct cggttcgtgc gtcggcggca tcggccaggc
     1321 gatcgagcag tgggagacgc tcaaggagcg cggctggaac cggatgtcgc cttacttcct
     1381 cccgaacttc ctcgtggact cgccgagcgg gtacctggcg atggagctcg gcctgaaggg
     1441 gccgaactgc gcggtcgtat cggcttgtgc gaccggctcg caatcgatcg gcgagggcgc
     1501 ggacctgatc cggagcggcc aagccgacgc cgtgctcgcg ggcggaacgg agggcgcgat
     1561 cacgcctctc atcctcggcg gcttctgctc gatgcggggg ctcgtccacg aggaggaaga
     1621 cccgacgcgc gcgtcgcggc cgttcgacgc cacccgggcc gggttcgtca tggccgaggg
     1681 cgccgctgtc ctcttgctcg aggagctcga cgcggcaaag gagcgcggcg ccacgattta
     1741 cgcggaggtc ctcggtcacg gcgcctcgaa cgacgcgtac cacatggcgc agcccgatcc
     1801 cgaagcgacc ggcgtcgtgg cgatgatgcg gcaggcgctc gcgatcgcgg aggttgagcc
     1861 cgggcgcgtc gactacatca acgcccacgg aaccgcgacg ccgctcggag acgtggccga
     1921 gacgaaggcg ctccgcgagg tgttcggcga ccacgccgac gagctcgccg tgtcgtcgac
     1981 caagtcgatg atcggccacc tcttcggtgc cgcgggggcg gtcgaggcg
//
LOCUS       DASZOB010000328         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_168235, whole genome shotgun sequence.
ACCESSION   DASZOB010000328 DASZOB010000000
VERSION     DASZOB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168235"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="sepH"
                     /locus_tag="VG709_05285"
     CDS             <1..790
                     /gene="sepH"
                     /locus_tag="VG709_05285"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HWC32524.1"
                     /translation="TRRDDRGNDATPAPEENDSVRSTRRRRGGDETGAGAPEAPREQP
                     ETKPDRPPVQPRTKRPQIPARSKLSPAEIQTLIRAGRTVRSVAQMAGTPTEWVKWLAQ
                     PVQEERRAIVANALRERQTRARLGASAEPLGASVARNLRSRGVSGADRVVDEGFTAVR
                     SNGREWRVRLSFEHRGRRTSAVWGYDPQRHEVTPLNTLAAQLGWRRPKSAAASVERDD
                     GVRATRAPARTAAARSRKTASSKSTSSRRRGGGRLSARRGRSSR"
     gene            complement(787..1680)
                     /locus_tag="VG709_05290"
     CDS             complement(787..1680)
                     /locus_tag="VG709_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32525.1"
                     /translation="MTIDEQVAALSTGRAFCELRGWWTTVVTGADARSWLGDLVTAAV
                     ETLAHGDAVRSLLLSPTGRMRADFTIAAVAGGFLLVQDPIQPARLEALLAPYVLSSDV
                     SLEDRSEELAVFAFPGHAPRSDKGERYTPSALGAGTDVVVNGSEAHHLRDAVANLLEV
                     DLDGVETWRIVRGAARFAVDLSEDSLPHEAALDDAIAYAKGCFLGQEAVAKVRNLGRP
                     PFVVVALRSDVEVRRGDRIVARGEEVGAVTSAASVSGAWSAIGRVRWAARDAELRTRS
                     GAALRSLGPASGADPKQGARR"
     gene            complement(1677..2615)
                     /locus_tag="VG709_05295"
     CDS             complement(1677..2615)
                     /locus_tag="VG709_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32526.1"
                     /translation="MDWTELVAADPDGTFFHTPRYLKVYWEELCRGTLEIALVRDGDD
                     LVAAAAFEIADGVLTFLGGSEVTDYMGPVALPGARERAAKGLAGALASRDDWGKADLS
                     GLLAEGAWAPALSAAADDVGFATLVDGDGAAPFLELPGTYDEYLASLSAKRRHEIRRK
                     TKRLAERLGAPAIADSKAETLSEGLDSFMRWHRQSAGAKGRFMAPGMELFFRRLGEEF
                     LSDGTFRLSFLEVDGTRLAGTIGFRDGRRFLLYNSAYDHDRRAIAPGIVMVAELVRDA
                     IASGCEAIDFLHGDLEYKYRFGARPRRLLRLQLERR"
     gene            complement(2694..>3020)
                     /locus_tag="VG709_05300"
     CDS             complement(2694..>3020)
                     /locus_tag="VG709_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32527.1"
                     /translation="VLMLFVLHHIERWSDQERLVREAARIARRRIVLIEDTPASSLDR
                     AFNVAWDWALNLRHGVPKPFTFRTADGWRRVFSRAGLRTMRAESYRARWPTLMTYHHT
                     LFVLER"
BASE COUNT          460 a         1100 c         1088 g          372 t
ORIGIN      
        1 gacccgacgg gacgatcgcg gcaacgacgc gacgcctgct ccggaggaga acgactccgt
       61 ccgctcaacg cggcggcgac gcggcgggga tgagacaggt gccggtgccc ccgaggcgcc
      121 gcgagagcag cccgagacga aacccgaccg cccgcccgtc cagccgagga cgaagcggcc
      181 gcagatcccc gcgcgctcga agctaagccc cgcggagatc cagacgctca tccgcgccgg
      241 tcggaccgtg aggagcgtcg cgcagatggc ggggacgccg accgagtggg tgaagtggct
      301 ggcgcagccg gtccaggaag agcggcgcgc gatcgtcgcg aacgcgctcc gcgagcgtca
      361 gacgagggcc cgcctcggcg cctccgccga gccgctcgga gcctcggtag cgcggaacct
      421 gcggagccgt ggcgtgtccg gcgccgaccg tgtcgtcgac gagggattca cggccgtgcg
      481 ctcgaacggc agggagtggc gtgtccggct ctcgttcgag catcgcggcc gccggacgag
      541 cgccgtgtgg ggctacgacc cgcagcggca cgaggtcacg ccgctcaaca cgctcgcggc
      601 gcagctcggg tggcggcgcc cgaagtcggc cgcggcgtcg gtcgagcggg acgacggcgt
      661 ccgagcgact cgcgctcctg cgcggaccgc ggcggcgcgc tcgcgcaaga cagcctcgtc
      721 gaagtcgacg tcctcgcgcc gacgcggcgg cggacggctc tcggcccggc gcggacgctc
      781 gtcgcgctag cggcgcgcgc cttgcttcgg atctgctccg gaggccggcc cgagcgaacg
      841 cagggccgcg ccggagcgtg tgcggagctc ggcgtcgcga gccgcccagc gaacgcgtcc
      901 gatcgcgctc caggcgccgc tcaccgaggc tgcgctcgtg acggccccga cctcctcgcc
      961 gcgcgcgacg atccggtcac cgcgtcgaac ctccacgtcg gagcggagcg cgaccacgac
     1021 gaagggcggg cggccgaggt tgcgtacctt cgcgacggcc tcctggccga ggaagcatcc
     1081 cttggcgtag gcgatcgcgt cgtcgagcgc ggcttcgtgg gggagcgaat cctccgacag
     1141 atcgaccgcg aagcgagccg cgcctcgcac gatgcgccac gtctccacgc cgtcgaggtc
     1201 gacctccaag aggttcgcca cggcgtcacg gaggtggtgc gcctcggacc cgttgacgac
     1261 gacgtcggtg ccggcgccga gcgccgacgg cgtgtaccgc tcgcccttat cgctgcgcgg
     1321 cgcgtgcccc gggaacgcga atacggcgag ctcttcgctc cggtcctcga gcgacacatc
     1381 cgacgacagc acgtacggcg ccagcagggc ctcgagccgt gccggctgta tcgggtcctg
     1441 gacgagcagg aacccgccgg cgaccgcggc gatcgtgaaa tcggcgcgca tcctcccggt
     1501 cggagacagc aggagcgatc ggaccgcgtc gccgtgcgcg agcgtctcca cggccgccgt
     1561 cacgaggtcg ccgagccacg aacgggcatc ggcaccggtg acgacggtcg tccaccaccc
     1621 gcgcagctcg cagaacgctc ggccggtcga gagcgcggcg acctgctcgt cgatggtcat
     1681 cgccgctcga gctggagccg aaggagccgg cgcgggcgcg cgccgaagcg gtacttgtac
     1741 tcgaggtcgc cgtggaggaa gtcgatcgcc tcgcaacccg acgcgatcgc gtcgcggacc
     1801 agctccgcga ccatgacgat ccccggcgcg atggcgcgcc ggtcgtggtc gtacgcggag
     1861 ttgtagagca agaagcgccg tccgtcgcgg aacccgatcg tccccgccag ccgcgtcccg
     1921 tcgacctcga ggaaggacag ccggaacgtt ccgtcggaga ggaactcctc gccgagccgg
     1981 cggaagaaga gctccatccc cggggccatg aaccgtccct tcgcgcccgc gctctgccgg
     2041 tgccaccgca tgaagctgtc gaggccctcg gagagcgtct cggccttcga gtcggcgatc
     2101 gccggcgccc cgagccgctc cgcaagtcgc ttcgtcttcc gccggatctc gtgacggcgt
     2161 ttcgcggaaa gcgacgcgag gtactcatcg tacgtcccag gtagctcgag gaacggcgcg
     2221 gcgccgtccc cgtcgacgag cgtcgcgaag cccacgtcgt cggccgcagc ggagagcgcc
     2281 ggcgcccacg ctccttccgc gaggagcccg gagagatccg ccttccccca gtcgtccctc
     2341 gacgcgagcg cgcccgcgag ccccttggcc gcgcgctcac gcgcgcccgg gagtgcgacc
     2401 ggccccatgt agtcggtgac ctcggagcca ccgaggaacg tcaacacgcc gtccgcgatc
     2461 tcgaacgccg ccgccgcgac gaggtcgtcg ccgtcgcgaa ccagcgcgat ctcgagcgtc
     2521 ccgcggcaga gctcctccca atagaccttc aggtagcgcg gcgtgtggaa gaacgtgccg
     2581 tccgggtccg cggcgacgag ctcggtccag tccacggcga cgaaggcctg gggatccgag
     2641 gtccacgtga cccgcacgaa ccaagggtac caacgggctg cgaccgccca tcttcagcgc
     2701 tcgagcacga agagcgtgtg gtggtacgtc atgagcgtcg gccaccgcgc gcggtagctc
     2761 tcggccctca tcgtgcgcag tcctgcgcgc gagaagacgc gtcgccatcc gtcggcggtc
     2821 cggaacgtga acggtttcgg gacgccgtgg cggaggttga gggcccagtc ccaggcgacg
     2881 ttgaaagctc gatccagcga cgacgcgggc gtgtcctcga tcagcacgat gcggcgtcgc
     2941 gcgatgcggg ccgcctcgcg caccaggcgc tcctggtcgc tccagcgctc gatgtggtgg
     3001 agcacgaaca gcatgaggac
//
LOCUS       DASZOB010000329         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_108341, whole genome shotgun sequence.
ACCESSION   DASZOB010000329 DASZOB010000000
VERSION     DASZOB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108341"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /gene="cgtA"
                     /locus_tag="VG709_05305"
     CDS             <1..149
                     /gene="cgtA"
                     /locus_tag="VG709_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HWC32528.1"
                     /translation="EDPRAVARLQDRLRKAGVERRLAEAGARRGDEIVIAGRPYEFIP
                     EEVP"
     gene            179..1255
                     /gene="proB"
                     /locus_tag="VG709_05310"
     CDS             179..1255
                     /gene="proB"
                     /locus_tag="VG709_05310"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWC32529.1"
                     /translation="MVKVGSSSLVDDAGVLAPRRLAKVVRDVAAVSRDRAAVLVSSGA
                     IAAGFRALGVRRRPRDMPGLQAAAAVGQGRLMADYARLFARQGVVAAQVLLTREDVVR
                     RRHFVNARNTLERLLSAGVLPVVNENDTVATEEIRFGDNDHLAALVAVMIRADLLVLL
                     SDVDGIYSRDPRRGGATLLEEVTDLDAVEATGPSSALGLGGMASKVEAARVATSAGTG
                     VVVARAGERDVIDRIVRGERVGTWVAPAGRRTRGRKAWIAFASGVRGRIVVDAGAERA
                     VRDDGRSLLAAGVVAVEGSFAAGDPVEVSGPSGAPFARGIVGYSSEELPRLAGRSTKD
                     LAAAPGGPSDGEVTPRDELVVVGR"
     gene            1252..>1609
                     /locus_tag="VG709_05315"
     CDS             1252..>1609
                     /locus_tag="VG709_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HWC32530.1"
                     /translation="MSAPEPLAGPARDAAAALATATTEAKNRALAAMAEEFEARAGDI
                     LEPNAADVSAAEAEGAPPAVIDRLGLDEARVRQMADGLRDVAALDDPIGDVLDDRTLE
                     NGLRIRKVRVPLGVIGV"
BASE COUNT          191 a          588 c          634 g          196 t
ORIGIN      
        1 tcgaggatcc gcgtgcggtc gcgcgcctcc aggaccggct gcgcaaggct ggcgtcgagc
       61 gccgcctcgc ggaagccggc gcgcgtcgcg gcgacgagat cgtgatcgcg ggccggcctt
      121 acgagttcat cccggaggag gttccctgac gcgcaagctc ggcgacgtcc gccgcctcgt
      181 ggtcaaggtc gggtcgtcga gcctcgtcga cgacgcgggt gtactcgcgc cgcgccggct
      241 cgcgaaggtg gttcgcgacg tcgcggcggt cagccgggac cgcgccgcgg tcctcgtctc
      301 gtcgggcgcg atcgcggcgg ggttccgggc gctcggcgtc cggcgcaggc cgcgggatat
      361 gccgggcctc caggccgccg ccgccgtcgg gcaagggcgg ctcatggccg actacgcgcg
      421 gctgttcgcg aggcagggcg tcgtcgccgc gcaggtattg ctcacgcgcg aggacgtcgt
      481 gcggcggcgc cacttcgtga acgctcgcaa cacgctcgag cgcctgctgt ccgccggcgt
      541 cctgccggtc gtcaacgaga acgacacggt cgcgaccgag gagatccgct tcggcgacaa
      601 cgaccacctc gccgcgctcg tagccgtgat gatccgcgcc gacctcctcg tgctgctctc
      661 cgacgtcgac gggatctact cgcgtgaccc gcggcgagga ggcgcgacgc tcctcgagga
      721 ggtcaccgac ctcgacgccg tcgaggcgac gggcccgtcg agcgccctcg ggctcggtgg
      781 gatggcgagc aaggtcgagg cggcgcgcgt cgcgacctcc gccgggacgg gcgtcgtcgt
      841 cgcacgggcg ggggagcgcg acgtcatcga ccgcatcgtg cgaggtgagc gggtcgggac
      901 gtgggtcgcg ccggcgggac gacggacgcg aggccgcaag gcgtggatcg cgttcgcgag
      961 cggcgtccgc gggcgcatcg tcgtcgacgc gggggccgag cgggccgtgc gcgacgacgg
     1021 ccgcagcctt ctcgccgcgg gcgtcgtggc ggtggaggga tcgttcgccg ccggggaccc
     1081 cgtcgaggtc tccgggccct cgggcgcgcc gttcgcgcgc ggcatcgtcg ggtactcgag
     1141 cgaggagctc ccgcggctcg ccgggcggtc cacgaaggac ctcgccgccg cccccggcgg
     1201 cccgtccgat ggagaggtga ccccccggga cgagctcgtg gtcgtcgggc gatgagcgcg
     1261 cccgagccgc tcgccgggcc cgcgcgcgac gccgccgccg cgctcgcgac cgcgacgacc
     1321 gaggcgaaga accgggcgct cgccgccatg gccgaggagt tcgaggcgcg cgccggggac
     1381 atcctcgagc cgaacgcggc ggacgtctca gccgccgagg ccgagggcgc gccgcccgcc
     1441 gtcatcgacc gcctcgggct cgacgaggcc cgcgtgcggc agatggcgga cggcctccgc
     1501 gacgtcgccg cgctcgacga cccgatcggc gacgtcctcg acgaccgcac gctcgaaaac
     1561 ggcctccgga tccggaaggt ccgcgtgccg ctcggcgtga tcggcgtga
//
LOCUS       DASZOB010000330         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_7387,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000330 DASZOB010000000
VERSION     DASZOB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7387"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1721)
                     /locus_tag="VG709_05320"
     CDS             complement(<1..>1721)
                     /locus_tag="VG709_05320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWC32531.1"
                     /translation="TMTSILNWYVEEGTIFKGGSGSGINLSPIRSSKERLGGGGTASG
                     PVSFMRGADASAGTIKSGGKTRRAAKMVILDVDHPDIEDFIWCKAIEERKARVLRDAG
                     FDMDLDGKDSHSTQYQNANNSVRVTDEFMQAYLDDRDWKLKAVTTGEALETKRARDLM
                     RQIAHAAWECADPGMQYDTTINDWHTCPASGRINASNPCSEYMHLDNSACNLASLNLM
                     KFLHDDGSFDIPAFRHAVEIVFTAQEILVGNSDYPTDKIARNAKAFRQLGLGYANLGA
                     LLMARGLPYDSDAGRAWAGAITALMTGHAYATSARVAERMGAFAGYSPNRDAMLRVMR
                     KHRTAAEDIDPELVPEALLSSAKQAWDEAIALGDEHGYRNAQASVLAPTGTIGLMMDC
                     DTTGIEPDLALVKNKKLVGGGTMRIVNQTVPRALARLGYTPEQTKDIVDYIAEHNSIV
                     EAPHLREEHYPVFDTSMGDRAIHYLGHVRMMGAVQPFISGAISKTVNMPEDVTVEDVE
                     EMFIEGWRLGLKAVAIYRDNCKVAQPLAANKKQKDAAPISTPATPERKRLPSTRPSKT
                     FSFTVGD"
BASE COUNT          224 a          573 c          597 g          327 t
ORIGIN      
        1 gtcgccgacc gtgaacgaga aggtcttcga cggccgcgtc gagggcaggc gctttcgctc
       61 gggcgtcgcc ggggtcgaga tgggggcggc gtccttctgc ttcttgttgg cggcgagcgg
      121 ctgggcgacc ttgcagttgt cccggtagat cgctacggcc ttgagtccga gccgccagcc
      181 ctcgatgaac atctcctcga cgtcctcgac cgtgacgtcc tcgggcatgt tgacggtctt
      241 ggagatcgcg cccgagatga acggctgcac cgctcccatc atccggacgt gcccgaggta
      301 gtggatcgcc cggtctccca tcgacgtgtc gaacaccggg tagtgctcct cgcggaggtg
      361 cggcgcctcc acgatcgagt tgtgctcggc gatgtagtcg acgatgtcct tggtctgctc
      421 cggcgtgtag ccgagccggg cgagcgcccg gggcaccgtc tggttgacga tccgcatcgt
      481 cccgccgccg acgagcttct tgttcttgac gagcgcgagg tcgggctcga tgccggtcgt
      541 gtcgcagtcc atcatcaggc cgatcgtccc ggtcggggcg agcacgctgg cctgcgcgtt
      601 ccggtagccg tgctcgtcgc cgagcgcgat cgcctcgtcc cacgcctgct tcgccgagga
      661 caggagcgcc tcggggacga gctcgggatc tatgtcctcc gccgcggtcc ggtgcttgcg
      721 catcacgcgg agcatcgcgt cgcggttcgg cgagtagccg gcgaacgcac ccatcctctc
      781 ggcgacgcgc gcgctcgtcg cgtacgcgtg gccggtcatg agcgccgtga tcgcgccggc
      841 ccacgcccgt ccggcatccg agtcgtacgg cagcccgcgg gccatgagca gcgccccgag
      901 gttcgcgtag ccgagcccga gctggcggaa cgccttcgcg ttgcgcgcga tcttgtcggt
      961 cgggtagtcg gagttcccga cgaggatctc ctgcgccgtg aagacgatct cgacggcgtg
     1021 acggaaggcc gggatgtcga agctcccgtc gtcgtggagg aacttcatga ggttgaggct
     1081 cgcgaggttg cacgcggagt tgtcgaggtg catgtactcc gagcacgggt tgctcgcgtt
     1141 gatccgcccc gacgccgggc acgtgtgcca gtcgttgatc gtcgtgtcgt actgcatccc
     1201 gggatcggcg cactcccacg cggcgtgcgc gatctgccgc atgaggtcac gcgcgcgctt
     1261 cgtctcgagg gcctcacccg tggtcacggc cttcagcttc cagtcccggt cgtcgagata
     1321 ggcctgcatg aactcgtcgg tgacccggac ggagttgttg gcgttctggt actgggtcga
     1381 gtggctgtcc ttgccgtcga ggtccatgtc gaagccggcg tcgcgcagga cgcgggcctt
     1441 ccgctcctcg atggccttgc accagatgaa gtcctcgatg tcgggatggt cgacgtcgag
     1501 gatgaccatc ttggcggcac gccgcgtctt gccgcccgac ttgatcgtcc ccgcggacgc
     1561 gtcggcgccg cgcatgaacg acaccgggcc gctcgccgtc ccgccgccgc cgagccgctc
     1621 ttttgaggag cggatcgggc tcaggttgat gcccgagccg gagccgccct tgaagatcgt
     1681 cccctcctcg acgtaccagt tgaggatcga cgtcatcgtg t
//
LOCUS       DASZOB010000331         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_87750, whole genome shotgun sequence.
ACCESSION   DASZOB010000331 DASZOB010000000
VERSION     DASZOB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87750"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VG709_05325"
     CDS             <1..702
                     /locus_tag="VG709_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWC32532.1"
                     /translation="RQIIYADRRRILEGEDLKDQALEMVQEIVTGTVEQYVSREVYPE
                     EWDLDALMAAVASVYPTRVTQDEVRETADAGAVVDRCVADALAVYAEREEQLGPEAMR
                     QIERMVLLSITDNKWREHLYELDYLEEGIHLRAYGQKDPLVEFQREAYGMFDEMKGGI
                     VEEFVRYIYRVELAQPEQQQVARPRPRLVLSSGGGDGQPQQAPSQAISDKVPRNAPCP
                     CGSGKKYKKCHGATA"
     gene            786..1694
                     /gene="prfB"
                     /locus_tag="VG709_05330"
                     /pseudo
     CDS             786..1694
                     /gene="prfB"
                     /locus_tag="VG709_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          294 a          588 c          602 g          210 t
ORIGIN      
        1 cgccagatca tctacgcgga ccgccggcgc atcctcgagg gcgaggacct gaaggaccag
       61 gcgctcgaga tggtccagga gatcgtcacc ggcacggtcg agcagtacgt cagccgcgag
      121 gtctacccgg aggagtggga cctcgacgcg ctgatggctg cggtcgcgag cgtctacccg
      181 acccgcgtga cgcaggacga ggtccgcgag accgccgacg cgggcgcggt cgtcgaccgc
      241 tgcgtcgcgg acgcgctcgc cgtatacgcg gagcgcgagg agcagctcgg tcccgaggcc
      301 atgcgccaga tcgagcggat ggtgctgctc tcgatcaccg acaacaagtg gcgcgagcac
      361 ctctacgagc tcgactacct ggaggagggc atccacctcc gcgcctacgg gcagaaggac
      421 ccgctcgtcg agttccagcg cgaggcctac gggatgttcg acgagatgaa gggcgggatc
      481 gtcgaggagt tcgtccgcta catctaccgg gtcgagctgg cccagcccga gcagcagcag
      541 gtcgcgcgcc cgcgcccgcg gctggtgctc tcctccggcg gcggcgacgg ccagccgcag
      601 caggcgccgt cccaggcgat cagcgacaag gtcccgcgca acgcgccgtg tccctgcggc
      661 tccggcaaga agtacaagaa gtgccacggc gcgaccgcct gagcatctag catcgcggac
      721 cgtgagcgag taccgcgaga cgctgcgcga gctgagcgaa cggctgtcga acgcgaagga
      781 gtacctttga cgtcgacgcc aagcgcacgg agatcgccga gatcgagcgt cgcctctccg
      841 tccccgatgt ctggaacgac caggcgaacg cccagcggct gaaccagcgc ctcgccagcg
      901 tgcggcagga cgtcgaccgt cacgccgcgc tcgcgtcgcg tctggaggac ctccgcgcga
      961 tcgacgagct gctccgcgac gccgacgacc cggagctcgc gaaggagctc tccacgggcg
     1021 tgacctcgct cgcggccgag atcgaccgcc tcgagctggc gaccctgctc tccggggagt
     1081 acgacgcgaa cgacgccgtc gcgagcatcc acgccggcgc cggcggcacc gagagccagg
     1141 actgggcgga gatgctgctg cggatgtacc tgcgctgggc ggagcgcgag ggcttcgacc
     1201 gcgagctcga cgaggtgcag cccggcgacg aggccggcct caagagcgcg acgttcatcg
     1261 tacgcgggag gaacgcgtac gggctgctgt ccgccgagcg gggcgtacac cggctcgtcc
     1321 gcatctcgcc gttcgacgcg agcaagcgtc gccacacgtc gttcgcctcg ctcgacgtga
     1381 tcccgctgat cgaggaggag gtcgaggaga tcgagatcga cccgaaggac ctccggatcg
     1441 acacctaccg ctcgagcggg gcgggcggcc agcacgtgaa cgtgacggac tcggcggtgc
     1501 ggatcacgca cctgccgacg gggatcgccg ccgcctgcca gaacgagcgc tcgcagatgc
     1561 agaaccgggc ggtcgcgatg cgcatcctga aggcgcgcct cgccgacctg cagcgtcgcc
     1621 aacgccacga cgcgatcgag agcctgcggg gcgagcggcg cgacatcgag ttcggctcgc
     1681 agatccgttc ctac
//
LOCUS       DASZOB010000332         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_97932, whole genome shotgun sequence.
ACCESSION   DASZOB010000332 DASZOB010000000
VERSION     DASZOB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97932"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /gene="dnaE"
                     /locus_tag="VG709_05335"
     CDS             <1..>1608
                     /gene="dnaE"
                     /locus_tag="VG709_05335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWC32533.1"
                     /translation="IKEMGFADYFLIVWDFIQFAKRNGIGVGPGRGSAAGSLVAYCLE
                     ITDIDPIRYDLLFERFLNPGRKSMPDIDIDFSVHGRDRVINYVREKYGSDRVAQIITF
                     GTMMARAAVRDAGRVLDIPYGTVDRVAKLIPEGPKVYLDDCLKKGSELAQAYDQDEVV
                     RQIVDLAKPLEGLVRQDSIHAAGVVIGDRPLTEYIPLQQKGADQEVVTQFPMGDVEAL
                     GLLKMDFLGLRNLDVIDKAVELIGGVDVIGLPLDDPKTYELLQRGESTGVFQFESSGM
                     REALRLVKPTEFEDLIALVALYRPGPMSYIPVYARRKAGAEQVAFVDERLRPITEPTY
                     GICIYQEQYMEIAKQLAGFSPAEADDLRKAIGKKIHSLMASLKDKFLEGCAANGVTPA
                     VATQLWKDMEQAQDYSFNKSHAACYALIAYRTAWLKANYPAEYMAALISSVMNTKDKV
                     PFYVAACDELGIEVLPPDVNESHVDFAVVEGRIRFGLNAVKNVGETAARAIVSGREAG
                     GRFTSIWDFVERIDLQVVNKRALEALTK"
BASE COUNT          280 a          551 c          529 g          248 t
ORIGIN      
        1 atcaaggaga tgggcttcgc cgactacttc ctgatcgtct gggacttcat ccagttcgcg
       61 aagcgaaacg gcatcggcgt cgggcccggg cgcggctcgg ccgccggctc gctcgtcgcc
      121 tactgcctcg agatcacgga catcgacccg atccgctacg acctcctctt cgagcgcttc
      181 ctgaatccgg gccgcaagtc gatgcccgac atcgacatcg acttctcggt ccacgggcgc
      241 gaccgcgtca tcaactacgt gcgcgagaag tacggctccg accgcgtcgc ccagatcatc
      301 accttcggga cgatgatggc ccgcgccgcc gtccgcgacg ccgggcgcgt gctcgacatt
      361 ccgtacggca cggtcgaccg ggtcgcgaag ctcatccccg aggggccgaa ggtctacctg
      421 gacgactgcc tgaagaaggg ctccgagctc gcgcaggcct acgaccagga cgaggtcgtt
      481 cgccagatcg tcgatctcgc gaagccgctc gagggcctcg tgcgccagga ctcgatccac
      541 gcggccggcg tcgtgatcgg cgatcggccg ctcacggagt acatcccgct ccagcagaag
      601 ggcgccgacc aggaggtcgt gacccagttc cccatgggcg acgtggaggc cctcggcctc
      661 ctcaagatgg acttcctcgg gctcaggaac ctcgacgtga tcgacaaggc ggtcgagctc
      721 atcggcggcg tggacgtcat cgggctcccg ctcgacgacc cgaagacgta cgagctgctg
      781 cagcgaggcg agtcgaccgg cgtcttccag ttcgagtcgt ccggcatgcg ggaggccctc
      841 cggctcgtca agccgaccga gttcgaggac ctgatcgcgc tcgtcgccct ctaccggccc
      901 ggcccgatga gctacatccc ggtgtacgcg aggcggaagg cgggcgccga gcaggtcgcg
      961 ttcgtcgacg agcgcctgcg cccgatcacc gagccgacgt acgggatctg catctaccag
     1021 gagcaataca tggagatcgc caagcagctg gccggcttct cgccggccga ggcggacgac
     1081 ctccgcaagg cgatcggcaa gaagatccac tcgctcatgg cgtccctcaa ggacaagttc
     1141 ctcgagggct gcgccgcgaa cggcgtcacg cccgcggtgg cgacgcagct ctggaaggac
     1201 atggagcagg cgcaggacta ctccttcaac aagtcgcacg ccgcctgcta cgcgttgatc
     1261 gcctaccgga cggcctggct caaggcgaac tacccggccg agtacatggc cgcgctgatc
     1321 tcgtcggtca tgaacactaa ggacaaggtg ccgttctacg tcgccgcgtg cgacgagctc
     1381 gggatcgagg tgctgccccc ggacgtgaac gagtcgcacg tcgacttcgc cgtcgtcgag
     1441 ggtcggatcc gattcgggct gaacgcggtc aagaacgtcg gcgagacggc ggcccgcgcg
     1501 atcgtgtcgg gccgcgaggc gggcggccgc ttcacctcga tctgggactt cgtcgagcgc
     1561 atcgacctgc aagtcgtgaa caagcgggcg ctcgaggcgc tcacgaag
//
LOCUS       DASZOB010000333         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_175015, whole genome shotgun sequence.
ACCESSION   DASZOB010000333 DASZOB010000000
VERSION     DASZOB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175015"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VG709_05340"
     CDS             complement(<1..203)
                     /locus_tag="VG709_05340"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWC32534.1"
                     /translation="MADPRLERLADVIVSYSTRVRPGDLVVIDSTPLGAPIARETYRR
                     VLAAGGHPVVHVGVDAAAESLLR"
     gene            complement(196..1335)
                     /locus_tag="VG709_05345"
     CDS             complement(196..1335)
                     /locus_tag="VG709_05345"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWC32535.1"
                     /translation="MRDPRVDRLADLVVNYSLELREGQSVRIDGFDVAAPLAVALFRA
                     ALDSGAYPYTNMTLEGLAEILVAEGSDDQIRYISPVDWHEVEEVDAVVTIWSETNTRA
                     FTRADERHQAALISTRRRLSNRRWERIAKGAARWCGTLHPTHAHAQEAEMSLGEYEKF
                     YFAACHADSDDPAAHWRETARSLERRAEELRSVRELRIVGPDTDLRVGVEGRTWLAAD
                     GRFNMPDGEVFTSPVETATQGHIRYTFPAVFQGREVEDVRLRFEDGKVVAAEASSGAP
                     YLEQLLDLDAGARILGEVAFGLNYEIDRFTRNILLDEKIGGTMHLALGSAFAQAGGSN
                     TSALHWDMICDLREGGEVYADGELVWRSGAFLAEPSQLAAAGAGG"
     gene            complement(1332..>1980)
                     /locus_tag="VG709_05350"
     CDS             complement(1332..>1980)
                     /locus_tag="VG709_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC32536.1"
                     /translation="LTHGASVVLTVGSDCAVGKKTMAIELDREARARGVRSVFVPTGQ
                     TGIMIAGWGIAVDAVVADFLAGAAERLVVEGARRGDLLWVEGQGAILHPQFSGVTLGL
                     YHGSAPHLLVLCHLAGTTEIEGCPGHAIPPLAELVELHERLALPARPATVAAVALNTS
                     GLDESEARRAVEAAEDETGLPADDPVRFGPGRLVDAILAHLPAPSAPFRAGIRAG"
BASE COUNT          286 a          711 c          712 g          271 t
ORIGIN      
        1 tgccggagaa gcgactccgc ggccgcgtcg acgccgacgt gaaccaccgg atgtccgccg
       61 gcggcgagta cgcggcggta cgtctcacgc gcgatcggcg cgccgagcgg cgtcgagtcg
      121 atcacgacga ggtcgcccgg gcggacgcga gtcgagtagg agacgattac gtcggcgaga
      181 cgctcgaggc gcgggtcagc caccggcgcc cgccgcggcg agctgcgaag gctcggccag
      241 gaacgcgccg gaccgccaca cgagctcgcc gtctgcgtac acctcgccgc cctcgcggag
      301 gtcgcagatc atgtcccagt ggagcgccga ggtgttcgat ccgcccgcct gcgcgaacgc
      361 cgagccgagc gcgaggtgca tcgtcccgcc gatcttctcg tcgagcagga tgttccgcgt
      421 gaagcggtcg atttcgtagt tcagcccgaa cgcgacctcg ccgaggatcc gcgcgccggc
      481 gtcgaggtcc agaagctgct cgaggtacgg ggctccgctc gaggcctcgg ccgcgacgac
      541 cttgccgtcc tcgaagcgaa ggcggacgtc ctcgacctcc ctgccctgga agacggccgg
      601 gaacgtgtac cggatatgtc cctgcgtcgc cgtctcgacc gggctcgtga agacctcgcc
      661 gtcgggcatg ttgaagcggc cgtccgcggc gagccaggtc cggccctcga cgccgacgcg
      721 gaggtcggtg tccgggccga cgatccggag ctcgcgaacg gaccggagct cctccgcgcg
      781 ccgctcgagc gaccgtgcgg tctcccgcca gtgggcggcc gggtcgtctg agtcggcgtg
      841 gcaggcggcg aagtagaact tctcgtactc gccgagcgac atctccgcct cctgcgcgtg
      901 ggcgtgcgtc gggtgcagcg tcccgcacca gcgcgccgcg cccttcgcta tccgctccca
      961 gcgccggttc gagagccgtc gcctggtcga gatgagagcc gcctggtggc gctcgtctgc
     1021 acgggtgaac gccctggtgt tcgtctccga ccagatggtg acgaccgcgt cgacctcctc
     1081 gacctcgtgc cagtcgaccg gcgagatgta gcggatctgg tcgtcggacc cctcggcgac
     1141 caggatctcc gcgagccctt cgagcgtcat gttcgtgtac ggatacgcac ccgagtcgag
     1201 agcggcgcgg aagagcgcga cggcgagcgg agccgcgacg tcgaagccgt cgatgcggac
     1261 gctctggccc tcgcggagct cgagcgagta gttgacgacg aggtcggcga gcctgtcgac
     1321 gcgggggtcc ctcaccccgc ccttatacca gcgcgaaacg gcgccgacgg cgccggaaga
     1381 tgggcgagaa tcgcgtccac caaacggcca ggtccaaagc ggacagggtc gtctgcgggc
     1441 aaacccgtct cgtcctcggc ggcctcgacc gcgcgccgcg cctcgctctc gtcgaggccg
     1501 ctcgtgttca gcgcaacggc ggcgacggtt gcaggccgcg ccgggagcgc gagccgctcg
     1561 tggagctcga ccagctcggc gagcgggggg atcgcgtggc ccggacagcc ctcgatctcc
     1621 gtcgtgcccg cgaggtggca gagcacgagc aggtgggggg cgcttccgtg gtagaggccg
     1681 agcgtgacgc cggagaactg cgggtgcagg atcgcgccct ggccttcgac ccagaggagg
     1741 tcgccgcggc gcgcgccctc gacgaccagc cgctccgccg cgccggcgag gaagtcggcg
     1801 acgaccgcgt ccaccgcgat cccccagccg gcgatcatga tccccgtctg gcccgtcggg
     1861 acgaagaccg agcgcacccc gcgcgcccgc gcttcccggt cgagctcgat cgccatcgtc
     1921 ttcttgccga cggcgcaatc cgacccgacc gtgaggacga cgctcgcccc gtgcgtcagg
//
LOCUS       DASZOB010000334         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148266, whole genome shotgun sequence.
ACCESSION   DASZOB010000334 DASZOB010000000
VERSION     DASZOB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148266"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VG709_05355"
     CDS             complement(<1..397)
                     /locus_tag="VG709_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWC32537.1"
                     /translation="MSPPPEVTVVIPTRDRWSLLATAALPAALVQEDVDLEVVVVDDG
                     SRDETPERLAELTDPRLRVIRNLEPLGVAQARNAGIAAARGRWLAFLDDDDLWSPRKL
                     RTQLDAARAAGASFVYAGVINVDEYGSALG"
     gene            complement(381..1151)
                     /locus_tag="VG709_05360"
     CDS             complement(381..1151)
                     /locus_tag="VG709_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWC32538.1"
                     /translation="MASDTPSPRFAPELVAKMRRTRRRPRPTQFDYLHTRRLVDDLAA
                     ALARVGADAREVLDVYCGARPYDDLLPPGAAVTGLDLPGNRYGIADVVTDEFLPFADE
                     SFDLVMCIEAFHYVTDPAHGVKEIRRVLRPGGHALVAVPLVWEYDRTILEHRYTGPEL
                     ASLFAGWEDVTVVENGGRAVSWATLTGSLAGMIQWNLPDRRLLRLAVRPVFAGFYLLV
                     NALGFLLDRAERRYYRSSATLPMNLLLTARRPLREPAA"
     gene            complement(1132..1929)
                     /locus_tag="VG709_05365"
     CDS             complement(1132..1929)
                     /locus_tag="VG709_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWC32539.1"
                     /translation="MLAELLEEGVVARTDSVIAVCAGSSERDLFVELGFTNVLITNLD
                     ERIDDEAVFAPFRWSLQDAQSLNVPDASFDFAFVADGLHHCSSPHRALLEMYRVSRKG
                     LVVVESRDNLLMRVAASVGLTPDYELEAVVDNDFRWGGVDNTAIPNYIYRWTESEFRK
                     TISSFDPSGKHTFRFFYGLNLPFEQAKFKRTNVKFYALRLSEPLVRAFTRVFKKQRNT
                     LAMVALKPRVPQDLWPWLTTGPEGIEMDRAYAGTHFKTPDGVGERHP"
     gene            complement(2049..2183)
                     /locus_tag="VG709_05370"
     CDS             complement(2049..2183)
                     /locus_tag="VG709_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32540.1"
                     /translation="MSYAPRQEPWLEPVEVRRCDALLAWADRAVFVYRNPPACATGRA
                     "
     gene            2268..>2512
                     /locus_tag="VG709_05375"
     CDS             2268..>2512
                     /locus_tag="VG709_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWC32541.1"
                     /translation="MRRFTGRAALMWLGIAVSFIFAYLAVRDIDSAALADGLRRASLW
                     TLMPAALVLAIAIFLRAVRWRILLSPPYRPSTRVVTGA"
BASE COUNT          392 a          861 c          873 g          386 t
ORIGIN      
        1 cgccgagagc gctcccgtac tcgtcgacgt tgatgactcc cgcgtagacg aacgacgccc
       61 cggcggcccg agccgcgtcg agttgcgtgc gcagcttgcg cggcgaccag aggtcgtcgt
      121 cgtcgaggaa agcgagccac cttccgcggg cggccgcgat gccggcgttg cgcgcctgcg
      181 ccacgcccag cggttccagg ttcctgatga cgcgcagtcg cgggtccgtg agctcagcga
      241 gccgctcggg cgtctcgtcg cgcgagccgt cgtcgacgac cacgacctct aagtcgacgt
      301 cctcctgcac gagcgccgcc ggaagcgctg cagtggcgag caggctccag cggtcgcgcg
      361 tcgggatgac gaccgtgact tcaggcggcg ggctcacgaa gggggcgtcg ggcggtgagc
      421 aggaggttca tcgggagcgt cgccgaactc cggtagtagc gccgctcggc ccggtcgagc
      481 aggaaaccga gcgcgttcac gaggaggtag aagccggcga acaccgggcg gacggcgagg
      541 cgaagcagcc ggcggtcggg caggttccac tggatcatcc cggcgagcga gccggtgagc
      601 gttgcccacg acaccgcccg gccgccgttc tcgaccacgg tcacgtcctc ccaacctgca
      661 aacagcgatg cgagctcggg gcccgtgtag cggtgctcga ggatcgtgcg gtcgtactcc
      721 cacacgaggg ggacggcgac gagcgcgtgc ccgcctgggc gcagaactcg ccggatctcc
      781 ttcacgccat gcgcaggatc ggtcacgtag tggaaggcct cgatgcacat gaccaggtcg
      841 aacgactcgt ccgcaaacgg caggaactcg tccgtcacca cgtcggcgat cccatagcgg
      901 ttacccggga ggtcgaggcc cgtgaccgct gccccgggcg ggaggaggtc gtcgtacggg
      961 cgcgctccgc agtagacgtc gaggacctcc ctcgcgtccg cgccgacccg tgcgagtgcg
     1021 gccgccaggt cgtcgacgag gcggcgagtg tggaggtagt cgaactgcgt ggggcgaggc
     1081 cgccgccgcg tgcgccgcat cttggcgacg agctcgggcg cgaaccgcgg gctaggggtg
     1141 tcgctcgcca acgccgtcgg gagtcttgaa atgggttcct gcataggctc gatccatctc
     1201 gatcccctcc ggcccggtcg tcagccacgg ccagaggtcc tgcggaacgc gcggcttcag
     1261 ggcgaccatc gccagggtgt tgcgctgctt cttgaacacg cgcgtgaacg ccctcacgag
     1321 cggctcgctc aggcgcaggg cgtagaactt gacgttcgtc cgcttgaact tcgcctgctc
     1381 gaagggcagg ttcaggccgt agaagaagcg aaacgtgtgc ttcccggacg ggtcgaacga
     1441 gctgattgtc ttcctgaact cgctctcggt ccagcggtag atgtagttgg ggatcgccgt
     1501 gttgtcgacg ccgccccatc tgaagtcgtt gtcgacgaca gcctcgagct cgtagtccgg
     1561 cgtgaggccg accgatgccg cgacccgcat cagcaggttg tcgcgcgact cgacgaccac
     1621 gagtcccttc ctcgagaccc ggtacatctc gagcagcgcc ctgtgcggcg acgagcagtg
     1681 atggagccca tcggcgacga acgcgaagtc gaaggaggcg tccggcacgt tcaggctctg
     1741 ggcgtcctgg aggctccagc ggaacggagc gaacacggcc tcgtcgtcga tgcgctcgtc
     1801 gaggttcgtg atcaacacgt tcgtgaagcc gagctccacg aacaggtctc gctcgctcga
     1861 cccggcgcaa acggcgatga cggagtccgt cctggcgacc acgccttcct ccagaagctc
     1921 cgcaagcacc gactcgacga acggtgttcg caacgcacga ctcccctgat cggggctccc
     1981 cgcccattcg ggtgcccacc gaggacgcta tccggtcagg ggctcgggat tccggaggac
     2041 gggcgaagtc acgctcgccc ggtcgcgcag gcgggtgggt tccggtacac gaacacggct
     2101 cgatccgccc aggccaggag cgcgtcgcag cggcgcacct cgaccggctc gagccacggc
     2161 tcctggcgag gcgcgtagga catcaggatg cagttggacg ccgcgggaaa cttcaggacg
     2221 tcatcccgcg ggacccgccg ggtcgccgcg aatacgctct cgccgggatg aggcggttca
     2281 cgggtcgcgc ggcgctcatg tggctaggca ttgccgtctc gttcatcttc gcctacctcg
     2341 ccgtccgcga catcgattcg gccgccctcg ccgacggcct gcggcgggcc agcctgtgga
     2401 cgctgatgcc tgccgcgctc gtcctcgcca tcgccatctt cctgcgcgcg gtccgctggc
     2461 ggatcctgct ctcgccgcca taccggccct cgacccgcgt ggtcaccggg gc
//
LOCUS       DASZOB010000335         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_158420, whole genome shotgun sequence.
ACCESSION   DASZOB010000335 DASZOB010000000
VERSION     DASZOB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158420"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="ftsZ"
                     /locus_tag="VG709_05380"
     CDS             complement(<1..1109)
                     /gene="ftsZ"
                     /locus_tag="VG709_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWC32542.1"
                     /translation="MQAPQNYLAVIKVVGIGGGGVNAVNRMIEAGLKGVEFIAINTDA
                     QALLMSEADVKLDIGRETTRGLGSGADPEIGRRAAEEHRAEIEEILKGADMVFVTAGE
                     GGGTGTGGAPVVASIARDLGALTIGVVTRPFGFEGRRRAMQADTGIQELKESVDTLII
                     VPNDRLLTISDPETSMLDAFRMADQILYQGVEGIVNLIMTPGLINLDFADVRAVLQNA
                     GSALMGIGHGGGSNRAEDAAKNAISSPLLEASIDGARGVLLSVSGGSDLSLHEVNRAA
                     EAITRAAHPDANIIFGAVIDDALAEECRVTVIAAGFDRTMGPSLQRPSLLREAAELTE
                     EEEEMPVPSLLAEPDVEEDLFAPAPAPVPAAARRG"
     gene            complement(1305..2063)
                     /locus_tag="VG709_05385"
     CDS             complement(1305..2063)
                     /locus_tag="VG709_05385"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWC32543.1"
                     /translation="MTIDPRGRLRGASGAALVVVALITIAWLLLSSPLFRIAAIEVDG
                     NAQLSDRELRELSGARIGDNLVFLSTRDVERALESSPWIADADVARRLPNSLVIGVAE
                     RRAVAVADDATTTFVIGADGIVLGEADAPGALPSLGRYGHLIEGEPLPGSLAPVRVLA
                     SLPSSVTRVVDAAELEPDGVVVRFARGGYATFGPATDTRRKAAALEGVLAWAEDRGVV
                     FRYIDVRVPESPVLRPKGRGTRSVHPEITPAPRG"
     gene            complement(2060..>2358)
                     /locus_tag="VG709_05390"
     CDS             complement(2060..>2358)
                     /locus_tag="VG709_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HWC32544.1"
                     /translation="DEAREWRRATQPLAEPNCGSVFKNPPGDHAARLIESIGGKSMNV
                     GGARVSEKHANFIVAGEGATASDVVELIERLRARVRERHGVDLHPEVHVVRTPA"
BASE COUNT          329 a          870 c          787 g          372 t
ORIGIN      
        1 tcgccgcggc gcgcggccgc ggggaccggt gccggggcgg gcgcgaacag gtcctcctcg
       61 acgtccggct ccgccaggag cgacggaacc ggcatctcct cttcctcctc cgtcagctcc
      121 gccgcctcgc ggagcaagct cggccgctgg agcgacggac ccatcgtcct gtcgaacccg
      181 gcggcgatca ccgtgacgcg gcactcctct gcgagcgcgt cgtcgatcac ggcgccgaag
      241 atgatgttgg cgtcgggatg cgccgctcgc gtgatcgcct cggcggcacg gttgacctca
      301 tgcaggctga ggtcggagcc gccggagacc gagagcagca cgccacgggc gccgtcgatc
      361 gacgcctcca gcagcgggga cgagatcgcg ttcttcgcgg catcctccgc gcggttgctg
      421 ccgcctccgt gaccgatgcc catgagcgcg gacccggcgt tctggagcac ggcgcggaca
      481 tccgcgaagt cgaggttgat gagccccggc gtcatgatga ggttcacgat cccctcgacc
      541 ccctggtaca ggatctggtc ggccatccgg aacgcatcga gcatcgacgt ctccgggtcc
      601 gagatcgtga gcagccggtc gttcggcacg atgatcagcg tgtcgaccga ctccttgagc
      661 tcctggatcc ctgtgtcggc ctgcatcgcg cggcgtcggc cctcgaagcc gaacgggcgc
      721 gtcacgacgc cgatcgtgag cgctccgagg tcccgcgcga tcgacgcgac gacgggtgcg
      781 ccgccggtgc ccgtgccacc gccttcgccg gcggtgacga agaccatgtc ggcgcccttc
      841 aggatctcct cgatctcggc gcggtgctcc tcggccgcgc gccgcccgat ctcggggtcc
      901 gcgccggagc cgaggccgcg tgtggtctcg cgaccgatgt cgagcttcac gtccgcctcg
      961 gacatcagca gggcctgcgc gtcggtgttg atcgcgatga actcgacgcc cttgaggccc
     1021 gcttcgatca tgcggttcac ggcgttcacg ccgccgccac cgatcccgac gaccttgatg
     1081 accgccagat agttctgagg agcttgcatc atgtccctcg cctccgtgtc cctcggccac
     1141 ttcggcgccg gtcacccggg gggtgggcgg tccgggcccg ttctccccgg tttccccgct
     1201 ccgacctgcg gagatgctcc ggggggttca acctctactt gatgttcaac gttatgtaaa
     1261 cctgccgtac aggtgcggaa ggtacacccg ctcgtacgat tttgtcaacc ccgaggtgcg
     1321 ggcgtgatct ccgggtgtac ggagcgggtg ccgcgtccct tgggccgaag cacggggctc
     1381 tccgggaccc gcacgtcgat gtaccggaac acgacgccgc ggtcctccgc ccacgcgagc
     1441 accccttcga gcgcggcggc cttccgccgc gtgtcggtcg cggggccgaa ggtcgcgtag
     1501 cccccgcgcg cgaagcggac gacgacgccg tcgggctcga gctctgccgc gtcgaccact
     1561 cgcgtcacag acgagggcaa cgacgcgagc acccgcaccg gcgccagcga acccgggagc
     1621 ggctccccct cgatgaggtg tccgtaccgt ccgagcgacg gtagggcccc cggcgcgtcg
     1681 gcctcgccga gcacgatccc gtcggccccg atcacgaagg tcgtcgtagc atcgtccgcg
     1741 accgcgacgg ctctccgctc ggccacgccg atgacgaggc tgttcgggag ccgacgggcc
     1801 acgtcggcat cggcgatcca cggcgacgac tcgagcgcgc gttcgacgtc tcgcgtcgac
     1861 aggaatacga ggttgtcgcc gatccgcgcg ccgctcagtt cccgcagctc ccggtccgag
     1921 agctgcgcgt tgccgtccac ctcgatcgcg gcgatccgga acagcggtga cgacagcagg
     1981 agccacgcga tcgtgatgag cgcgactacg accagcgccg caccgctcgc gcccctgaga
     2041 cgacctcgcg gatcgatcgt cacgccggcg tgcggacgac gtggacctcg ggatgcaggt
     2101 cgacgccgtg ccgctcccgg acgcgtgctc tgagccgctc gatcagctcc acgacatccg
     2161 acgcggtcgc tccctcccct gccacgatga agttcgcgtg cttctcggac acgcgtgcgc
     2221 cgccgacgtt catcgacttc ccgccgatcg attcgatgag gcgcgccgca tgatcgccag
     2281 gcgggttctt gaacacgctg ccgcagttcg gctcggcgag cggctgcgtc gcccgccgcc
     2341 attcgcgtgc ctcatcca
//
LOCUS       DASZOB010000336         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148280, whole genome shotgun sequence.
ACCESSION   DASZOB010000336 DASZOB010000000
VERSION     DASZOB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148280"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VG709_05395"
     CDS             <1..983
                     /locus_tag="VG709_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWC32545.1"
                     /translation="VTYVRGEGVVLFEPSVIAVDDETGAVQAVGEDAKRMIGRTPASI
                     RATRPLRNGVITDFEVTEQMLRHFIRKTIGSRFRNPRVIVCVPSGITEVERRAVVEAT
                     VSAGAAHAALIEEPMAAAIGARLPIAEPAGNMVVDVGGGTTEIAVISLGGMVVWESLR
                     LGGYEMDEAIVRHVKREHGLVIGQEGAEAVKVAIGSAFDGHSGQAEVRGRDAVSGLLR
                     RKALRAEEVREALEPPVAAIVQAVKDTLERTPPELAADIVARGIVLAGGGCLLRGLPE
                     RLAAETDLPVQLVEEPLTCVAVGAGQSLDDFDAIAKAADAKSYPARGLFS"
     gene            complement(1007..1195)
                     /locus_tag="VG709_05400"
     CDS             complement(1007..1195)
                     /locus_tag="VG709_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32546.1"
                     /translation="MEEPRSAVLSPERPEEEPRAASPKAYRNVAAGRHVFRVAARDAD
                     GDLDLTPAVRGWRVVPRG"
     gene            1345..>1972
                     /locus_tag="VG709_05405"
     CDS             1345..>1972
                     /locus_tag="VG709_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HWC32547.1"
                     /translation="MAVKTTDAEVERTESVLALVRERVPDEVAPQVEEFVRQYYAGAA
                     PEDLLEHDVLDTYGAAVAHRHLAAERRPGEAKVRVYTPDLEEHGWQSTHTVVEIVNDD
                     MPFLVDSVSAEVDRHGIGIHLVLHPVLMVRRDEEGRLLEVVPPDDPPDDAIAESLIHV
                     EADRQTEPHVLEQLEADLRRVLGDVRAAVEDWQAMRQRALEIVAALESE"
BASE COUNT          285 a          692 c          734 g          261 t
ORIGIN      
        1 ccgtcacgta cgtccgcggc gaaggggtcg tgctcttcga gccctcggtg atcgcggtcg
       61 acgacgagac gggcgccgtc caggcggtcg gcgaggacgc gaagcggatg atcgggcgga
      121 cccccgcgtc gatccgcgcc acccggccgc ttcgaaacgg cgtcatcacc gacttcgagg
      181 tgaccgagca gatgctccgt cacttcatcc ggaagacgat cggaagccgg ttccggaacc
      241 cgcgcgtgat cgtgtgcgtg ccgagcggga tcacggaggt cgagcgccgc gcggtcgtcg
      301 aggcgacggt gtcggccggc gcagcgcacg ccgcgctgat cgaggagccg atggcggcgg
      361 cgatcggcgc gcggctcccg atcgccgagc cggcggggaa catggtcgtc gacgtcggcg
      421 ggggcacgac ggagatcgcc gtcatctcgc tgggcgggat ggtcgtgtgg gagtcgctcc
      481 ggctcggcgg ctacgagatg gacgaggcga tcgtccgcca cgtcaagcgc gagcacgggc
      541 tcgtgatcgg ccaggagggc gcggaagctg tcaaggtggc gatcggctcg gccttcgacg
      601 gtcactcggg ccaggcggag gtacgcggcc gcgacgccgt ctccggcctg ctccggcgga
      661 aggcgctccg cgccgaggag gtgcgcgaag cgctggagcc gccggtcgcc gcgatcgtgc
      721 aggcggtcaa ggacacgctg gagcggacgc cgcccgagct cgccgcggac atcgtcgcgc
      781 gcgggatcgt cctcgccggg ggcggctgcc tgctgcgcgg ccttccagag cgcctggccg
      841 cggagacgga cctccccgtg cagctcgtcg aagaaccgct cacgtgcgtg gcagtcggcg
      901 ccggccagag cctcgacgac ttcgacgcga tcgccaaggc cgcggatgcg aagagctacc
      961 ccgcgcgcgg gctcttctcg tagcccggtt ccgctaccgc gtcacctcag ccgcgcggca
     1021 cgacccgcca gccgcggacg gccggcgtca ggtcgaggtc gccgtcggca tcgcgcgcgg
     1081 cgacgcggaa gacgtggcgg ccggcggcca cgttccgata cgccttcggc gacgccgcac
     1141 gaggctcctc ctcaggtcgc tctggtgaca ggacggcgct gcgcggctcc tccaaggtct
     1201 cgagtagtga cgcccagaca agcagctcga tgcgcttgtt acatccgcga tttccctaag
     1261 atccgggcgg acgctcgtgc gactccgacc cgttctcggc gaagtgcgag gctgggcgtc
     1321 cgcccggccc ggtcaggggt accgatggcc gtgaagacga ccgacgccga ggtggagcgg
     1381 accgagagcg tcctcgcgct cgtccgcgag cgggttcccg acgaggtcgc gccgcaggtc
     1441 gaggagttcg tgcggcagta ctacgccggg gcggcgccgg aggatctgct cgagcacgac
     1501 gtcctcgaca cgtacggagc ggcggtcgcg catcgtcacc tcgccgccga gcggcggccc
     1561 ggcgaggcga aggtgcgcgt ctacacgccc gacctcgagg agcacggctg gcagtcgacg
     1621 cacacggtcg tcgagatcgt gaacgacgac atgccgttcc tcgtcgactc cgtaagcgcc
     1681 gaggtcgacc gccacgggat cgggatccac ctcgtcctcc atcccgtgct catggtgcgg
     1741 cgcgacgagg aaggtcgact gctcgaggtc gtgcctccgg acgacccgcc cgacgacgcg
     1801 atcgccgagt cgctcatcca cgtcgaggcc gaccgccaga ccgagcccca cgtcctcgag
     1861 cagctggagg ccgacctgcg ccgcgtcctc ggcgacgtcc gcgccgcggt cgaggactgg
     1921 caggcgatgc gccagcgggc gctcgagatc gtcgccgcgc tcgagtccga gc
//
LOCUS       DASZOB010000337         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_33667, whole genome shotgun sequence.
ACCESSION   DASZOB010000337 DASZOB010000000
VERSION     DASZOB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33667"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VG709_05410"
     CDS             <1..669
                     /locus_tag="VG709_05410"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWC32548.1"
                     /translation="YLLYPDAEELTPEARARLSALADHTELGAGFSIAMRDLEIRGAG
                     NLLGGEQSGHIAAVGFDTYARLLAESVAELKGEPVPEEREIRIDLPVRAFLPVEYVGE
                     ERLRMDLYRRISAARTHAELDAVRDEVRDRFGDLPAEVETLVEVAGLRIACAERGIDE
                     VSTVRGQVRLRPLDADPARLPDGATYHAATRTLNLAPPAQRLGRNLPAWVRRRLGSVA
                     SGGV"
     gene            680..1654
                     /locus_tag="VG709_05415"
     CDS             680..1654
                     /locus_tag="VG709_05415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWC32549.1"
                     /translation="MRSSLKLAFAAALLLPACGLSTTAAAVVNGDQIDAEQFQRQLDF
                     LLADPRLQQEVPAEGGEEQRQEFTRQFLTFLIHQQLLHEYADDNGIEVPQDEVRQQLE
                     QQIESLGGEEQFEAQLANAQATRADVEALIREQVLRQQVAEEIASQAVTEEQLRETYE
                     ERRAEFTTAEVSHILVQTRAEAERIAAQATADNFARLARRFSQDPGSAGRGGDLGRQP
                     LGGLVGPFAEAVLETPEGEVGGPVQTQFGWHVVLVRSLDAEPFDVVRDRLLEETRGRA
                     FTEWLQERVREAEIRVNPRYGLFDEQSGQVVPRTQTSPSPAPTVQVVP"
     gene            1651..>2216
                     /locus_tag="VG709_05420"
     CDS             1651..>2216
                     /locus_tag="VG709_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HWC32550.1"
                     /translation="MTRREPLPTPDPLSHVPTGDVPSSRDGDRLLDLVRVVGRLRGPG
                     GCPWDRAQTHASLRRHLLDETYELLDAIEAEDPHRLRDELGDVLLQAVFHAEIARDAG
                     EFDIDDVAEGTVEKLIRRHPHVFADTTAETPSEVYANWERIKAAEEGAAGTIDAGIPR
                     ALPALLAAQKVQRRAAGRGFDWAELSGAV"
BASE COUNT          340 a          783 c          802 g          291 t
ORIGIN      
        1 tacctgctct atcccgacgc ggaggagctg acgcccgagg cgcgggcgcg cctctccgcg
       61 ctcgccgacc ataccgagct cggcgccggc ttctcgatcg cgatgcgcga cctcgagatc
      121 cgcggggccg gaaacctgct cggcggcgag cagtcggggc acatcgccgc ggtcggcttc
      181 gacacgtacg cgcgtctgct cgcagagagc gtcgccgagc tcaagggcga accggtaccc
      241 gaggagcgcg agatccggat cgacctgccg gtccgcgcct tcctcccggt cgagtacgtc
      301 ggcgaggaac ggctgcgcat ggacctctac cgtcggatct ccgctgcgcg gacccacgcg
      361 gagctcgacg ccgtgcgcga tgaggtgcgg gaccgtttcg gcgacctgcc ggccgaagtc
      421 gagacgctgg tcgaggtcgc ggggctccgc atcgcatgcg ccgagcgggg catcgacgag
      481 gtgtcgacgg tgcgcggcca ggtccgccta cgcccgctcg acgccgaccc ggctcgcttg
      541 cccgacggcg cgacctacca tgcggcgacg cgcacgttga acctcgcgcc gcccgcccag
      601 cggctcggcc ggaacctccc ggcctgggtg cggcgacggc tcggaagcgt ggcgagcggg
      661 ggcgtctaga atcgaacgga tgcgttcctc gctcaagctg gcgttcgccg ctgcgttgct
      721 tcttccggcg tgcggactct cgacgaccgc tgccgccgtc gtcaacggcg atcagatcga
      781 cgccgaacag ttccagcgac agctcgactt cctgcttgcg gaccccaggc tccagcagga
      841 ggtcccggcc gagggcggcg aggagcagcg gcaggagttc acacggcagt tcctgacgtt
      901 cctgatccat cagcagctcc tgcacgagta cgcggatgac aacgggatcg aggtcccgca
      961 ggacgaggtt cgccagcagc tcgagcagca gatcgagtcg ctcggtggag aggaacagtt
     1021 cgaagcgcag ctcgcgaacg cgcaagcgac tcgggccgac gtcgaggcgc tgatccgcga
     1081 gcaggtcctc cgccaacagg tggcggagga gatcgcgtcg caggccgtca cggaggagca
     1141 gctgcgcgag acctacgagg agcgacgagc cgagttcacg acggcggagg tgtcgcacat
     1201 cctcgtccag accagagccg aggccgaacg catcgcggcc caggccacgg ccgacaactt
     1261 cgcgcgcctg gcgcgacgct tctcgcagga ccccggatcc gcgggacgcg gcggcgatct
     1321 cggccgccag ccgctgggcg ggctcgtagg cccgttcgcg gaagccgtcc tcgagacgcc
     1381 ggagggcgag gtcggaggcc ccgtccagac ccaattcgga tggcacgtcg tcctcgtccg
     1441 ctcgctcgac gccgagccgt tcgacgtcgt cagagaccgg ctcctcgagg agacgcgcgg
     1501 gcgtgccttc acggagtggc tgcaggagcg cgtccgcgag gcggagatcc gcgtgaaccc
     1561 gagatatggg ctgttcgacg agcagagcgg gcaggtcgtc ccgcggacgc agacgtcccc
     1621 gagtccggca cccacggtcc aggtcgtccc gtgacgcggc gggagccgct cccgacgccc
     1681 gatccgctgt cacacgtgcc gacgggcgac gtcccgtcga gccgcgacgg cgatcgtctc
     1741 ctcgacctcg tcagggtcgt cgggcgcctc cgcgggccgg gcggatgccc ttgggaccgc
     1801 gcccagacgc acgcgagcct gcgtcggcat ctgctcgacg agacgtacga gcttctggac
     1861 gcgatcgagg cggaggaccc gcaccgcctg cgcgacgagc tcggcgacgt cctcctccag
     1921 gcggtgttcc atgcggagat cgcgcgcgac gccggcgagt tcgacatcga cgatgtcgcg
     1981 gaggggaccg tcgagaagct gatccgccgg cacccacacg tcttcgcgga cacgaccgcc
     2041 gagacgccgt cggaggtcta cgcgaactgg gagcgcatca aggccgcgga ggagggggcc
     2101 gccggcacga tcgacgccgg tatcccgcgc gcgctgcccg ccctgttggc ggcgcagaag
     2161 gtgcagcgcc gggccgccgg acgcgggttc gactgggcag agctctccgg ggccgt
//
LOCUS       DASZOB010000338         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_117445, whole genome shotgun sequence.
ACCESSION   DASZOB010000338 DASZOB010000000
VERSION     DASZOB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117445"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..760)
                     /gene="hpt"
                     /locus_tag="VG709_05425"
     CDS             complement(206..760)
                     /gene="hpt"
                     /locus_tag="VG709_05425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWC32551.1"
                     /translation="MTAGVAEVLIEEATLQERIGVLGGELTRDYRGRDLFLVGVLKGA
                     IFFMADLMRRIEVPCEVDFMAISSYGAGVDSSGVVRILKDLDISIEGRNVLVVEDIID
                     SGLTLSYLLRNLEAREPASLEVCALLTKPERREHGVVCRYVGFEIPNRFVIGYGLDFA
                     ERYRNLPYVGVLRDELAAQAEATA"
     gene            complement(807..1820)
                     /gene="tilS"
                     /locus_tag="VG709_05430"
     CDS             complement(807..1820)
                     /gene="tilS"
                     /locus_tag="VG709_05430"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWC32552.1"
                     /translation="MDAAALRARIEEHVRAHDLLPPGGDVVCLVSGGADSTCLWHALT
                     TLGYRVQALHVDHKLRGAESDEDARFCVERFGARVVEAPAATGTEAELRDLRYSFATG
                     RARATGHTASDQVETILYRLATSGRPTGIRVRRDDGVVRPLLTVWREETEAYCRAEKL
                     PFRVDGSNADTVRGLIRRDVVPALRRLHPAAEANVLAALAEPAPLPRPVERALAELIT
                     SREGSKRVDLGAGKVAVREYEHVWIERAPVPLDGPVTWGPWTIEARRPGLRVRAWRAG
                     DRLAERDKKVHDLFVDAKVPRSERDAWPLVVRGDEVVAVPGVASARGWEGAVTARRRE
                     TID"
     gene            complement(1885..>2394)
                     /locus_tag="VG709_05435"
     CDS             complement(1885..>2394)
                     /locus_tag="VG709_05435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32553.1"
                     /translation="LRDRLPRPLDDRPGQPVYVLEEAPVRGHSGLRAATFDDLDILVP
                     AAAHAYLEEVGVDAYARDPDLFEWRTRSQVEQGRSWIWREDGHVLFKAEASAWTDDVV
                     QLQQVWVEPALRGRGYARRALSDLCRLLLARTPTVCLFVRPENAPAIALYETIGMRRT
                     ITYRSLIFP"
BASE COUNT          361 a          833 c          881 g          319 t
ORIGIN      
        1 cgacggtgtc gatcagctgg ctgtacggga tcttggtgcg ctcgtccttg ttcggcagga
       61 gcgtctgcga ggccaggtag accagcagga cgatgacgat gagcggaaag agtgcgctac
      121 ggaagaaacg gctcaacgag ctcctttgcg attaacggta gcagcctctt attcggctcc
      181 cggcgcgggc gggatgagcg cgacgctacg ccgtcgcctc ggcctgggcc gcgagctcgt
      241 cccggaggac gcccacgtag ggcaggttgc ggtagcgctc ggcgaagtcg aggccgtacc
      301 cgatgacaaa ccggttcggg atctcgaacc cgacgtagcg gcagacgacg ccgtgctcgc
      361 gccgttcggg cttcgtcagg agcgcgcaga cctcgagcga ggccggctcg cgcgcctcca
      421 ggttgcggag caggtacgag agcgtcagcc ccgaatcgat gatgtcctcg acgacgagca
      481 cgttgcgccc ctcgatcgag atgtcgaggt ccttgaggat ccggacgacg cccgaggagt
      541 cgacgccggc gccgtagctc gagatggcca tgaagtcgac ctcgcacgga acctcgatcc
      601 ggcgcatgag atccgccatg aagaagatcg cgcccttgag gacgccgacg aggaagaggt
      661 cgcgtccgcg gtagtcgcgg gtgagctcgc cgccgagcac gccgatccgc tcctggagcg
      721 tcgcctcctc gatcaggacc tcggcgacgc ctgcggtcag ctcgtcggcc gcgagatcgt
      781 cgcgaacggc gctggcccgg accggctcag tcaatcgtct ccctcctgcg cgcggtgact
      841 gcgccttccc atccgcgcgc ggacgcaacc cccggaacgg cgacgacctc gtccccccgg
      901 acgacgagcg gccaggcgtc gcgctcggac cgcggcacct tcgcgtcgac gaagagatcg
      961 tggaccttct tgtcgcgctc ggcgagccgg tcaccggcgc gccaggcgcg gacgcgcagg
     1021 ccgggccgcc tcgcctcgat cgtccacggg ccccaggtca cggggccgtc gagaggcacg
     1081 ggcgcccgct cgatccagac gtgctcgtac tcccggacgg cgaccttccc tgcgccgagg
     1141 tcgacgcgct tggaaccctc ccgcgacgtg atcagctcgg cgagagcgcg ctcgaccggg
     1201 cgcgggagcg gcgcgggctc ggcgagcgcg gcgaggacat tcgcctcggc cgccggatgg
     1261 agccgccgca gggcgggcac gacgtcgcgg cggatgagcc cgcggacggt gtcggcgttc
     1321 gagccgtcga cgcgaaacgg aagcttctct gcacggcagt acgcctcggt ctcctcacgc
     1381 cagacggtga ggagcggacg cacgacgccg tcgtcgcggc gcacgcggat cccggtcggg
     1441 cggccgctcg tcgcgaggcg gtagaggatc gtctcgacct ggtcggaggc ggtgtggccg
     1501 gtcgcgcgcg cgcggcccgt cgcgaaggag tagcggaggt cgcggagctc ggcctccgtg
     1561 cccgtcgcgg ccggcgcctc gaccacgcgc gccccgaagc gctcgacgca gaagcgggca
     1621 tcctcgtccg actcggcgcc ccggagcttg tggtcgacgt gcagcgcctg cacgcggtat
     1681 ccgagcgtcg tgagcgcgtg ccagagacac gtcgagtcgg caccgcccga gacgagacag
     1741 acgacgtcgc cgccgggcgg aagcaggtcg tgcgcgcgga cgtgctcctc gatgcgggcg
     1801 cggagcgccg cggcgtccat ccgcgcattc taggcgtcac cgggaggcgc ggcgcggggc
     1861 aacgcgccgc ggaggggcac gcccctacgg aaagatcagc gagcggtacg tgatcgtccg
     1921 gcgcatgccg atcgtctcgt acaaggcgat cgccggtgcg ttctccggcc tgacgaagag
     1981 gcataccgtg ggcgtgcgcg cgagcaggag ccggcagagg tcggagaggg cgcgtctcgc
     2041 gtacccgcgt ccacgcagcg cgggctcaac ccacacctgc tggagctgca cgacgtcgtc
     2101 ggtccacgcc gaggcctccg ccttgaagag gacgtgaccg tcctcgcgcc agatccagct
     2161 ccgcccctgc tccacctgag accgcgtgcg ccactcgaag aggtccgggt cgcgcgcgta
     2221 cgcgtccacg ccgacctcct cgaggtacgc gtgcgcggcc gcgggaacga ggatgtcgag
     2281 atcgtcgaac gtggcggcgc gaaggccgct gtgcccgcgg acgggcgcct cctcgagcac
     2341 gtacaccggt tgcccggggc ggtcgtcgag cggtcgcggc aggcggtctc gaag
//
LOCUS       DASZOB010000339         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148406, whole genome shotgun sequence.
ACCESSION   DASZOB010000339 DASZOB010000000
VERSION     DASZOB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148406"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..643)
                     /locus_tag="VG709_05440"
     CDS             complement(50..643)
                     /locus_tag="VG709_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32554.1"
                     /translation="MAGVVAAAIVLGAGTWFMLSREGGPLHRPDVPRFSFQVVEVGAA
                     GVTGGAHRAALGPAVDAIRQTLDALYVGGFIDPGRWANGEFPALADAFAGDATARARE
                     DVADLTLGPAAEEVSSVNPIGGRLDVRFLLGDEGNPFAAWATTVFRARGVAEGGDGLA
                     IEHRGRYLLRPVEDRWLIVGYDVTGRLRPGPPARATP"
     gene            820..>2286
                     /locus_tag="VG709_05445"
     CDS             820..>2286
                     /locus_tag="VG709_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWC32555.1"
                     /translation="MYVLAQEGGPIDVVFGSFEDTSLLVILGISLFALVVAYVLMRGV
                     LAAPEGTPTMREIAHAIQTGSRAYLNRQFRTVGLFLGILAIVLFFVLPVPEDATHTAL
                     SIKLGRSIAFVLGAGFSAVTGFAGMWLAVRGNVRVANAARESGLRRAMTLAFRTGGVA
                     GMFTVGLGLLGAAAILMIYKQDATSVLVGFGFGGALLAMFMRVCGGIYTKAADVGADL
                     VGKVEKNIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVTLVASIILGAAAFAG
                     SPQGAIVGVMFPLFVRAIGVITSIVGIYAVRSRSEEEHGMRAINRGFFFSAILSAAAV
                     YFVADIYVDDLRTFWAVVIGLVLASVISVLTEHFTSTTRAPVREIAASSQTGAATTIL
                     SGFSVGLESTVYSILVIAATVIGAFLLGNDTAERLYFISLTGMGMLTTVGVIVSMDTY
                     GPVSDNAHGIAEMAGGLGEEADEIMAGLDAVGNTTKAIT"
BASE COUNT          310 a          851 c          770 g          355 t
ORIGIN      
        1 atccacgcca cgagcgcgac cagcgccgcc gcgccgagcc accaacgcct cacggcgtcg
       61 ccctcgccgg cggccccgga cgcagccgcc ccgtgacgtc gtatccgacg atcagccacc
      121 ggtcctcgac cggccgaagc aggtagcgac cgcggtgctc gatcgcgaga ccgtcgccgc
      181 cttcggcgac gccgcgcgcg cggaagaccg tcgtcgccca tgccgcgaac gggttgccct
      241 catcaccgag gaggaaccgc acgtcgagcc ggccgccgat cgggttgacg gacgacacct
      301 cctccgccgc cggcccgagc gtcaggtccg cgacgtcctc gcgggcccgc gcggtcgcgt
      361 cgccggcgaa cgcgtcggcg agtgcgggga actcgccgtt cgcccatcgt ccgggatcga
      421 tgaacccccc gacgtagagc gcgtccagcg tctgccggat cgcgtcgacg gccgggccga
      481 gcgccgccct gtgcgcgccg cccgtcacgc ccgcggcgcc gacctcgacg acctggaacg
      541 agaaccgcgg cacgtccggc cgatggagtg gcccgccctc tcgcgacagc atgaaccacg
      601 tgccggcgcc gaggacgatg gccgcggcga ccacgcccgc cacgacggtc cacggccggg
      661 catcccgagc ttcccccgcc ccttgcaacg gctgggcagc gtaggaccgc ctccgccgtt
      721 cggcaacccc gggcggagcc gtgggcggag ccgctcgagg actggctata atccggcggc
      781 cctgacctgg gcgtcgcccg caaatccgca ggaggctcca tgtacgtgct ggctcaggaa
      841 ggcggaccga tcgacgtcgt cttcggatcg ttcgaagaca ccagcttgct cgtcatcctc
      901 ggcatctcgc tgttcgcgct ggtcgtcgcg tatgtgctga tgcggggtgt gctcgcggcc
      961 cccgagggga cgccgacgat gcgcgagatc gcccatgcga tccagaccgg cagccgcgcg
     1021 tacctgaacc gtcagttccg gaccgtcggg ctgttcctcg ggatcctcgc gatcgtgctg
     1081 ttcttcgtgc ttcccgtgcc agaggacgcg acgcacacgg ccctctcgat caagctcggc
     1141 cgctcgatcg cgttcgtcct cggcgccggg ttctccgccg tgacggggtt cgccggcatg
     1201 tggctcgccg tgcgcgggaa cgtccgcgtc gccaacgccg cgcgcgagtc cgggctccgc
     1261 cgcgcgatga cgctcgcgtt ccgcaccggc ggcgtcgccg ggatgttcac ggtcgggctc
     1321 ggcctcctcg gggccgccgc gatcctcatg atctacaagc aggacgcgac gtcggtgctc
     1381 gtcgggttcg ggttcggcgg cgcgctgctc gcgatgttca tgcgggtctg cgggggcatc
     1441 tacacgaagg ccgccgacgt cggggccgac ctcgtcggca aggtcgagaa gaacatcccc
     1501 gaggacgacc ctcgcaacgc cgccacgatc gccgacaacg tcggcgacaa cgtcggcgac
     1561 tgcgccggga tggccgccga cctgttcgag tcgtacgaag tgacgctcgt cgcctcgatc
     1621 atcctcggcg ccgccgcgtt cgcggggtcg ccgcagggcg cgatcgttgg cgtgatgttc
     1681 ccgctgttcg tccgtgcgat cggcgtcatc acgtcgatcg tcgggatcta cgccgtccgc
     1741 tcccgctccg aggaggagca cgggatgcgc gcgatcaacc gcggcttctt cttctcggcg
     1801 attctgtccg cggcggccgt ctacttcgtc gccgacatct acgtcgatga cctccgcacg
     1861 ttctgggccg tcgtgatcgg gctcgtgctc gccagcgtga tctccgtcct caccgagcac
     1921 ttcacgtcga cgacgcgcgc gcccgtccgc gagatcgccg cctcgtccca gaccggcgcc
     1981 gcgacgacga tcctgtcggg gttctccgtc gggctcgagt cgacggtgta ctcgatcctc
     2041 gtcatcgcgg cgaccgtgat cggggcgttc cttctcggga acgacactgc ggagcgcctg
     2101 tacttcatct cgctcaccgg catggggatg cttacgaccg tcggcgtcat cgtgtcgatg
     2161 gacacctacg gcccggtctc ggacaacgcg catggcatcg cggagatggc cggtggcctc
     2221 ggcgaggagg ccgacgagat catggccggc ctcgacgcgg tcgggaacac caccaaggcg
     2281 atcacc
//
LOCUS       DASZOB010000340         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_158567, whole genome shotgun sequence.
ACCESSION   DASZOB010000340 DASZOB010000000
VERSION     DASZOB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158567"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VG709_05450"
     CDS             complement(<1..1535)
                     /locus_tag="VG709_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWC32556.1"
                     /translation="MPRTEQPAIGSYALIGDTRSAALCSAEGAIDWLCIPRFDSEPVF
                     SRLVAGERGGSFSVRPRNGRPAGRGYLPGSAVLETSWRADGSELSVTDGMVVDVEGAL
                     LPRLVLVRRLEARGAPIEVDIVFDPRRGLPGRAPDRVARRGTTVVSSWGSLAVAITTD
                     ADARLQPGTAEHVTVDPSRPVTLAMTVAHREPLVLVEPEAAYRMLRRTDEAWRNWSQR
                     LDYGGLRPSTVERSLVTLRLLTYSPSGAAVAAPTTSLPEAIGAGRNWDYRYSWPRDSS
                     LGIEAFLGVGRSEEPQTYLHWLLHASRITRPRVRVLYSLDGRPGSEEVEADVPGYRGS
                     RPVRLGNGASDQHQLDVYGWGLRAAATMEARGERLDGETWRAMADLADFVASRWRDPD
                     AGIWERRGSPAHHVHSKLMAWLALDRALRIASSRRVRRRRVSRWSEAREAITAEVRRK
                     GWDDDAGAYVAAFGDRSLDAAVLVPLVEWEDRGSPRFASTVDAIASRLSAGRPLLYRY
                     APET"
     gene            complement(1577..2440)
                     /locus_tag="VG709_05455"
     CDS             complement(1577..2440)
                     /locus_tag="VG709_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWC32557.1"
                     /translation="MLQNRGLTRAIERLNAVDDLAATLQRAVGKAVPQQSRRKDLLSG
                     TWLGEPVHPPLTDVVIGCWTSAVLLDVFGGEGSEAAARKLGGLGVRAAVPTAASGASD
                     WAELGDDVRRVGVVHASGNTAALSLFALSYLARARGRAGAGRLLSALGYGVAVFSAYL
                     GGYLSFGRGVGVSQVAFEQLPGEWTAVMDADGLEEGKPARAALDGVGVLLVRTRGGVH
                     AIADRCSHRGCSLADGSIEDGTVVCPCHGSAFRLESGAIVKGPATSPQPALETRVREG
                     KIEVRRHEDAS"
     gene            complement(2473..>3433)
                     /locus_tag="VG709_05460"
     CDS             complement(2473..>3433)
                     /locus_tag="VG709_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32558.1"
                     /translation="AGRDRLSAVVGGVVLGLWWATYGHFLSPGGLPDNPLRLFVDQTP
                     PATVRWLDYTELWLISLPIYIVVSVLVLLLDARLVTRGGGIRRPLAATAALASILLVT
                     GATVSPDEYGQTVEVNASGSAQVETGDFYSGDFAGATADIDVGATDIGGRVTPLPPHD
                     RLAIDASVQAAGGTFQVEVRQPMVNDPLGRFTTWWGVGLDVDHHGHTGIGTNELPETH
                     SELAVFGVGDVATDGETIARGVPVHVMTLDDGSLELDVGDPGVGPIAGLPDGHLRVRW
                     AAYDGEVSSVPRNARYVGGGLFVLALLVALGWLVRREPARVEG"
BASE COUNT          497 a         1300 c         1207 g          429 t
ORIGIN      
        1 gtctcgggcg cgtagcggta gaggagcggg cgcccggccg acaggcggct cgcgatcgcg
       61 tccacggtcg aggcgaagcg cggggagccg cggtcctccc actcgacgag cgggacgagc
      121 acggcggcgt cgagcgaacg atccccgaac gcggcgacat aggcgccggc atcgtcgtcc
      181 caacccttgc gccgaacctc ggcggtgatc gcctcgcgcg cctcgctcca gcgagagacg
      241 cggcggcggc gcacgcggcg cgaggaggcg atccgcagcg cccggtcgag tgcgagccac
      301 gccatgagct tcgagtgaac gtggtgtgcg ggcgaccctc gccgctccca gatcccggcg
      361 tcggggtcgc gccagcggct cgcgacgaaa tcggcgagat cggccatcgc ccgccacgtc
      421 tcgccgtcca gccgctcacc ccgcgcctcc atcgtcgccg cggcgcgcag gccccatcca
      481 tagacgtcga gctggtgttg gtccgaagcg ccgttgccga ggcgcacggg acggctacct
      541 cggtaacccg ggacgtctgc ctcgacctcc tcggagccgg gcctcccgtc cagcgagtag
      601 agcacgcgga cgcgtggccg cgtgatgcgg ctcgcgtgca acagccagtg gaggtacgtc
      661 tgaggctcct cggaccgccc gacgccgagg aacgcctcga tgccgaggct ggagtccctc
      721 ggccacgagt accggtagtc ccagttgcgc cccgcaccga tcgcctcggg gagcgacgta
      781 gtcggagccg cgaccgccgc accggacggc gagtacgtga ggaggcgcag cgtgacgagg
      841 ctccgctcga cggtcgaagg acggaggccg ccgtagtcga gccgctgcga ccagtttcgc
      901 cacgcctcat cggtccggcg gagcatgcgg tatgccgcct ccggctcgac gaggacgagc
      961 ggctcccggt gcgcgacggt catcgcgagc gtcaccggac gcgacggatc cacggtgaca
     1021 tgctcggcgg tgccgggctg gaggcgcgca tcggcgtcgg tcgtgatcgc gaccgcgagc
     1081 gacccccacg acgagacgac cgtggtcccg cggcgggcga cccggtcggg ggctctcccc
     1141 ggcaggccgc gccgcgggtc aaagacgatg tcgacctcga tcggcgcgcc tcgggcctcc
     1201 aggcgtcgga cgaggacgag ccgtgggagc agggcgccct cgacgtccac caccatgccg
     1261 tcggtcacgg agagctccga gccgtccgcg cgccacgacg tctcgagcac cgcggacccg
     1321 ggaaggtacc cacggccggc gggccggccg ttccgcgggc gtaccgagaa gctcccgccc
     1381 cgctcgccgg ccacgagccg agagaacacc ggttccgagt cgaaccgggg gatgcagagc
     1441 cagtcgatcg ccccctctgc cgagcacagg gcggcgctcc tcgtgtcgcc gatgagcgcg
     1501 tacgagccga tcgccggctg ctcggtgcgc ggcatccggc gaggctagcg caggtccctt
     1561 gcggcgcagc ctcggctcac gacgcgtcct cgtggcgacg gacctcgatc ttgccctcgc
     1621 ggaccctcgt ctcgagcgcg ggctgcggcg acgtcgccgg gcccttcacg atcgcgcctg
     1681 actcgagccg gaacgcgctg ccgtggcacg ggcagacgac ggtgccgtct tcgatggatc
     1741 catcggccag cgagcatccg cggtggctgc agcggtcggc gatcgcgtgg acgccccccc
     1801 tcgtccggac gagaagaacc cccacgccgt cgagcgccgc ccgcgccggc ttcccttcct
     1861 cgagcccgtc ggcatccatg acggcggtcc actcgccggg aagctgctcg aacgccacct
     1921 ggctcactcc gacgccccgg ccgaacgaca ggtagccgcc gaggtacgcc gagaagaccg
     1981 ccacgccgta gccgagcgcc gacagcaagc gtccggcacc ggcccgcccg cgcgcacgcg
     2041 cgaggtacga gagcgcgaag agcgacagcg ccgcggtgtt gccgctcgca tgcactacgc
     2101 cgacgcgacg gacgtcgtcg cccagctccg cccagtccga tgcgccgctc gcggccgtcg
     2161 gaaccgccgc gcgcacgccc agcccgccga gcttccgcgc cgcggcttcg ctcccttcgc
     2221 caccgaagac atcgagcagg accgcggacg tccagcaacc gatcaccaca tcggtaagcg
     2281 gcggatggac cggctcgccg agccacgtgc ccgagagcag gtccttccgc cggctctgct
     2341 gcgggaccgc cttcccgacg gctcgctgga gggtcgcggc gaggtcgtcg accgcgttca
     2401 gccgctcgat cgctctggtc agtccgcggt tctgcagcat cgggtcagcc tcctcgatcc
     2461 tttgacgggc gcttacccct ccacgcgggc gggctcacgt cggacgagcc acccgagcgc
     2521 gacgaggagc gcgagcacga acagtccgcc ccccacgtac cgggcgttcc tcggcacgct
     2581 cgacacctcg ccgtcgtacg cggcccaccg cacgcgcagg tgcccgtcgg gtagcccggc
     2641 gatcgggccg acgcctgggt cgcccacgtc gagctcgagc gacccgtcgt caagcgtcat
     2701 cacgtgcacc gggactccgc gcgcgatcgt ctctccatcc gtcgctacat cgccgacgcc
     2761 gaacacggcg agctcggagt gcgtctccgg gagctcgttg gtgccgatcc cggtgtggcc
     2821 gtggtggtcc acgtcgaggc cgacgcccca ccacgtcgtg aagcgcccga gcgggtcgtt
     2881 caccatcggc tgccggacct cgacttggaa cgttccgcca gccgcctgga ccgacgcgtc
     2941 gatcgcgagg cggtcgtgcg gcgggagcgg cgtcacgcgc cccccgatgt cggtcgcgcc
     3001 gacgtcgatg tcggcggtcg cgccggcgaa gtcgccgctg tagaagtcgc cggtctcgac
     3061 ctgcgccgac ccggacgcgt tcacctcgac cgtctgtccg tactcgtccg gggacacggt
     3121 cgccccggtg acgaggagga tgctggctag cgccgccgtc gcggcgagcg ggcggcggat
     3181 cccgccgcct cgcgtcacga gccgcgcgtc gaggagcagc acgagcaccg acacgacgat
     3241 gtagatcggg aggctgatca gccacagctc cgtgtagtcg agccagcgga ccgtcgccgg
     3301 cggcgtctgg tccacgaaca gcctgagcgg gttgtcgggg agccctccag gcgacaggaa
     3361 atggccgtac gtggcccacc agagcccgag caccacccct ccaaccacgg cggagagccg
     3421 gtcgcgtccc gcg
//
LOCUS       DASZOB010000341         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_158571, whole genome shotgun sequence.
ACCESSION   DASZOB010000341 DASZOB010000000
VERSION     DASZOB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158571"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VG709_05465"
     CDS             complement(<1..893)
                     /locus_tag="VG709_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32559.1"
                     /translation="MTVLAHAGTSLLSFGTDWLYVLVPLGLYGAGALWMWRRGATDEG
                     RPIRRPVARIGSSLEDVTGLPGWVAAGLATGLMALVIAVLGFLWDVSWHIDLGRDEFL
                     FTPAHTAILVGLAMIAAAGVVAIAFATVEDADVGFRVGPLRVPFSGAALAVLGGGSLL
                     GFPIDELWHSAYGIDVTMWGPTHLVMIGGASLAPIALWLMVTEAGPGAGRAGYVRRAR
                     LLLAGGVLTGLSTFQAEFDFGAPQFQQLYHPVLVSIAAGIALVAARSAVGRGGALVAV
                     AVFAAIRSVLAWIVGPVFDLT"
     gene            complement(899..2059)
                     /locus_tag="VG709_05470"
     CDS             complement(899..2059)
                     /locus_tag="VG709_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32560.1"
                     /translation="MQRSSDPDTDEELTVDTTLTRPTTFGPIEAFDGPTLEETPAEQL
                     RRIRDRAPDFREWFRDTGAVTAFAARSLVTLPYPRRYALWEACRSPVPYVWMTNRMFV
                     IQWDEDGRTRTLVAEPSDYDLGVGTPFLRKTVERLPLSWQRSLDTFFRRHGTIEGHLA
                     DFGIAPEDVDYVTFDHLHTQDIRRIVGTRGPAPDLGSADAPVPPLFPNAKLIVQPEEL
                     EHVRDVHPFQSRFHQPWTYDDIREDNVLFIEGDVLVAPGLALLRTPGHTFGNHTIVVN
                     TERGIFTSSENGIAVECYSPEHSRLPGVREWARRWGLEVVMNFNTPEFASWQYNSMIK
                     EKLIADRVPERPEFVQVFPSSELVSNPLAPGIRPTYRHGDLTIGTVRPSAAA"
BASE COUNT          362 a          775 c          754 g          281 t
ORIGIN      
        1 atcgtgaggt cgaagaccgg cccgacgatc cacgcgagca cggagcggat ggccgcgaag
       61 accgccaccg cgacgagcgc gccgcccctg ccgaccgccg agcgcgccgc gaccagcgcg
      121 atcccggccg cgatcgagac cagcaccgga tggtagagct gctggaactg cggcgcgccg
      181 aagtcgaact cggcctggaa cgtcgacagc cccgtgagga cgccgccggc cagcagcagg
      241 cgagcgcgac ggacgtagcc ggcgcggccc gcaccggggc cggcctccgt caccatgagc
      301 cacagcgcga tcggggccag cgaggctccg ccgatcatca cgaggtgcgt cggcccccac
      361 atcgtcacgt cgatgccgta ggcgctgtgc cacagctcgt cgatagggaa ccccagcaac
      421 gagccgccgc cgagcacggc gagcgccgcg ccggagaacg gcacgcgcag cggaccgacg
      481 cggaagccca cgtccgcgtc ctcgacggtc gcgaacgcga tcgcgacgac gccggccgct
      541 gcgatcatcg cgagcccgac gaggattgcc gtgtgggccg gcgtgaaaag gaactcgtcg
      601 cgcccgaggt cgatgtgcca cgacacgtcc cacaggaacc cgagcacggc gatgacgagc
      661 gccatgaggc cggtcgcgag ccccgccgcg acccacccgg gcaggcccgt cacgtcctcg
      721 agcgaggagc cgatcctcgc tacgggacgc cggatcggcc gcccctcgtc cgtcgcgccg
      781 cgcctccaca tccacagcgc gccggccccg tagaggccga gcgggacgag cacgtacagc
      841 cagtcggtcc cgaacgagag cagcgacgtc cctgcgtgcg ccagcacggt catcgacatc
      901 acgccgcggc gctcggacgc accgtgccga tcgtcaggtc gccgtgccgg tacgtcgggc
      961 ggatccccgg tgcgagcgga ttcgacacga gctccgagga cgggaagacc tgcacgaact
     1021 cggggcgctc gggcacccgg tccgcgatca gcttctcctt gatcatcgag ttgtactgcc
     1081 agctagcgaa ctccggtgtg ttgaagttca tgacgacctc gagcccccac cgacgcgccc
     1141 actcgcggac cccggggagc ctcgagtgct cgggcgagta gcactcgacg gcgatcccgt
     1201 tctccgacga cgtgaagatc ccgcgctcgg tgttcacgac gatcgtgtgg ttgccaaacg
     1261 tgtgcccggg cgtccggagc agcgcgaggc cgggcgcaac caacacgtcc ccctcgatga
     1321 aaaggacgtt gtcctcgcgg atgtcgtcgt aggtccacgg ctggtggaac ctcgactgga
     1381 acgggtgcac gtcccggacg tgctcgagct cctcaggctg aacgatcagc ttcgcgttcg
     1441 ggaacagcgg cgggaccggc gcatcggcgg agccgaggtc cggcgcgggt ccgcgcgtcc
     1501 ccacgatccg gcggatgtcc tgggtgtgga ggtggtcgaa cgtcacgtag tcgacgtcct
     1561 ccggcgcgat gccgaagtcg gcgaggtgcc cctcgatcgt cccgtggcgg cggaagaacg
     1621 tgtcgagcga tcgctgccac gagagcggca ggcgctcgac ggtcttccgc aggaacggtg
     1681 tcccgacgcc gaggtcgtag tccgagggct cggcgaccag ggtcctcgtg cgcccgtcct
     1741 cgtcccactg gatcacgaac atgcggttcg tcatccagac gtagggcacc ggcgagcggc
     1801 atgcctccca cagcgcgtac cgcctcgggt atggaagcgt cacgagggat cgcgccgcga
     1861 aggccgtgac cgcccccgtg tcgcggaacc actcgcggaa atcgggcgcg cggtcacgga
     1921 tccgccgcag ctgctcggcg ggggtctcct cgagcgtcgg gccgtcgaag gcctcgatcg
     1981 ggccgaacgt cgtggggcgg gtcagcgtcg tgtccacggt gagctcctcg tcggtgtcgg
     2041 ggtcacttga ccgttgcacg gaatgatgta acatcagtcg ctgccatgtc aaccccggcg
     2101 gaagccctca cgatcgacca gctggccgcc acgaccggcg tcagctcgcg gaacatccgc
     2161 gcgtaccaga cc
//
LOCUS       DASZOB010000342         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_117498, whole genome shotgun sequence.
ACCESSION   DASZOB010000342 DASZOB010000000
VERSION     DASZOB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117498"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1238
                     /locus_tag="VG709_05475"
     CDS             186..1238
                     /locus_tag="VG709_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWC32561.1"
                     /translation="MDTFAAFRRAGTSLGSVADPVAAFARVFDAREALLRSALRLRDA
                     FRDGRVRTSTHTQTTCLDLAGVPSLHTARCTLSIDVGGNDRYRNNAGGTSGGAAAAID
                     LGGSDSYASTLNIGSNGGGSFGAGFLFDAAGNDSYVGGNQASNGGGYLLGAGFLLDAG
                     GSDRYTGRACTNGGACAFASGTLLDLGTRNDAYSAGGGAVNGGVLAVAHGMLLDAGGT
                     DTYSEGAPCYGSGVDRTVVPKGDGVGAQLDSTNPRGRTCIEPPPPPPPATIGMGAIWG
                     RVSAYGTGQGITGATVDCRRAGTGVTTVDGRYSIPAAPGGSFRCTATASGFRPQSRDV
                     TVRPGYTTIANFALRR"
     gene            complement(1261..>1540)
                     /locus_tag="VG709_05480"
     CDS             complement(1261..>1540)
                     /locus_tag="VG709_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32562.1"
                     /translation="MVDEARRHRLFRRLEDVLGAEEAESLMEHLPPLGWGDVATKQDL
                     ARLEDRLRRDMLEISKRDLRTIITTTITANSVSILAVAGVAFAAARLT"
BASE COUNT          203 a          571 c          550 g          216 t
ORIGIN      
        1 aggccgctgg atccggcgac gcgctcgtcg cgacgagagc cgccacgacc ggttggcggg
       61 cggcgttccg gcgggcccgg gacgtaccgc ccgccgcctc gctagcgacc cgccgatccc
      121 ccgccatcgc gtttcgttcc gtcgaaggcg tcacgcctcg ccagcggcgc gcgctcggag
      181 cggtcatgga caccttcgcg gcgttccgcc gcgcgggtac gtcgctcggt tcggtcgccg
      241 atcccgtcgc cgctttcgcg cgggtcttcg acgcgcgcga ggcgctccta cgctcggcgc
      301 tccgtctgcg ggacgccttc cgcgacggtc gcgtccgcac ctcgacgcac acgcagacca
      361 cgtgcctcga cctggccgga gtcccgagtc tccacacggc ccggtgcacg ctctcgatcg
      421 acgtcggcgg caacgaccgg taccgcaaca acgccggagg gacgtcgggc ggcgccgcgg
      481 cggcgatcga tctcggcggg agcgactcgt acgcgtcgac gctgaacatc gggtcgaacg
      541 gcggcgggag cttcggcgcc ggcttcctgt tcgatgcggc cggcaacgac tcgtacgtcg
      601 gtggcaacca ggcgtcgaac ggcggcgggt acctgctcgg tgctggcttc ctcctcgacg
      661 ccggcgggag cgaccgctac accggccgag cgtgcacgaa cggcggcgcg tgcgcgttcg
      721 cgtcggggac gctgctcgac ctcgggaccc ggaacgacgc gtactccgca ggtggcggcg
      781 cggtgaacgg cggcgtcctc gcggtggctc acggcatgct gctcgatgcc ggcgggaccg
      841 acacgtacag cgagggagcg ccatgctacg gctccggcgt cgatcgaacc gtcgtgccga
      901 agggcgacgg cgtcggcgca cagctcgact cgacgaaccc gcgcggacgg acgtgcatcg
      961 agccaccgcc gccgcctccg ccggccacga tcggtatggg agcgatctgg ggtcgggtga
     1021 gcgcgtacgg caccggccag gggatcaccg gcgcgaccgt cgactgtcgg cgcgccggga
     1081 cgggcgtcac gaccgtcgac ggccgatact cgatccccgc cgcgcccgga gggtcgttcc
     1141 gctgcacggc gaccgcgagc ggcttccggc cccagagccg cgacgtcacc gttcgaccgg
     1201 gctacacgac gatcgcgaac ttcgccctcc gccgctgacc gacgggccgg tccgccggcg
     1261 tcaggtgagc cgggcggcgg cgaacgccac tccggccacc gcgaggatgc tcaccgagtt
     1321 ggccgtgatc gtcgtcgtga taatcgttcg gaggtctcgc ttcgagatct cgagcatgtc
     1381 acgccgcagc ctgtcctcca ggcgcgcgag gtcctgcttc gtcgccacat cgccccatcc
     1441 gaggggcggg aggtgctcca tcaaggactc tgcctcctcg gcgccgagga catcctcgag
     1501 ccggcggaac agccggtggc gtctcgcttc gtcgaccatt
//
LOCUS       DASZOB010000343         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_7650,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000343 DASZOB010000000
VERSION     DASZOB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7650"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VG709_05485"
     CDS             complement(<1..171)
                     /locus_tag="VG709_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006827634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWC32563.1"
                     /translation="MPEIGYTLSSEEQPPTDLVRLARRAEEVGFPFALISDHFHPWLD
                     TQGHSPFVWAVLG"
     gene            234..1262
                     /locus_tag="VG709_05490"
     CDS             234..1262
                     /locus_tag="VG709_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWC32564.1"
                     /translation="MPTNLEIAELLARESEVADTDRRRRALRRASRHALGWEQEAADV
                     VASGAPITTLSAVGPWIAGKIRAWLDDPPERPDPPPERAGFMTRAEARRILASSPDWS
                     SPRADLQVHTTWSDGHASLDEMVRVASTYGYDHVAITDHSKGLPIANGMDEPTLAEQG
                     REIARLNRTLDTAGAPIRALRSLEMNLSPEGDGDMEADALERLDLVLGAFHSKLRVRE
                     DQTRRYVAALRNPRVHVLAHPRGRRWSTRAGLSADWETVVTAASETRTALEIDAYPDR
                     QDLDVATLRLAAEAQAWISIGTDAHYPDELRFLELGLAAAVEAGIRRERVLNFLPRDD
                     LLAWARER"
     gene            1330..2019
                     /locus_tag="VG709_05495"
     CDS             1330..2019
                     /locus_tag="VG709_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWC32565.1"
                     /translation="MAVKTKAREAREKTLETFGTAAERGKDVLGTAAGRGKDVLGTAA
                     ERGKDVLGSATERAREYTPKRIRARREKQRRRERRRSLLKGLGVGLAVAYFFDPDNGN
                     RRRNIARDRVMALFRTGADRGARLGRYAASQTYGAVQKVRHAGPDAPPENDATLARKV
                     ESEVLGNSPLKGNVSVNAERGVVVLRGQIERPDDIRDLEDRVRKVSGVQDVRNLLHTP
                     GAPTPGRTTTG"
     gene            2023..2661
                     /locus_tag="VG709_05500"
     CDS             2023..2661
                     /locus_tag="VG709_05500"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HWC32566.1"
                     /translation="MPLEQTRIDREATSEETFLQILDEATAVLDRLEVPYGLIGGIAS
                     AVEGRPRWTNRGEDIDVFVRHKDAMRALEALAEAGFETEETNENWIYKGAKDGVLVDV
                     IFRSTGDIYLDDEMAARIQAGEFRGRVVKLIPPEDVLIMKAVAHGEETPQYWHDALSV
                     LARADIDWNYLLRRARQHGARRVLSLLIYAQSNDLVVPPEIVAELFDMVQPR"
     gene            2673..2936
                     /locus_tag="VG709_05505"
     CDS             2673..2936
                     /locus_tag="VG709_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWC32567.1"
                     /translation="MENPERAAREGLIAQVREALAEDPRVGEMEVKVELHGERLVILG
                     TVATPARRERISRIIAERFPGHDIDNRTEVPPLAEPEEAERLS"
     gene            2933..3676
                     /locus_tag="VG709_05510"
     CDS             2933..3676
                     /locus_tag="VG709_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWC32568.1"
                     /translation="MIRVAAVGDVHFGPDSRGNYRPSLSRLSERADVFLIAGDLTRWG
                     DPEEARALAEELHELPVPTFAVLGNHDYQSGREDDVVDVMAACGIRVLDGDGVTVEVG
                     GVRVGIAGTAGFGGGFAGGHATDFGEPETKAFVGRTIRMAGELEGALLDLDADVRIAL
                     LHYSPVRETLSGEPPEIHAFLGSYLLAEAIDRAGADLALHGHAHRGTEKGATPRGVHV
                     RNVAWPVIEKAYALYELEPRKARREQLVR"
     gene            complement(3680..4114)
                     /locus_tag="VG709_05515"
     CDS             complement(3680..4114)
                     /locus_tag="VG709_05515"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HWC32569.1"
                     /translation="MDTVLLGIGALLTGAVVIFLVATLFRHIYTERQRTGVRRIWDNF
                     GLGLTLMILFLTTWLAQGLVQWVQFVDEQRAHNEPTELGQFIAHFSQSTLENWQSEFL
                     QLFSFVVLAAMMIHHGSAESKDSTDRMERKIDEIKQMLQKKS"
     gene            complement(4176..4394)
                     /locus_tag="VG709_05520"
     CDS             complement(4176..4394)
                     /locus_tag="VG709_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32570.1"
                     /translation="MEERPDRSPEKDPTVDADEVEPEAPGPGDSEGARPEPDPDEAGE
                     DRTPPEPTGGIPDDAADSSPDAATPEGA"
     gene            4645..4974
                     /locus_tag="VG709_05525"
     CDS             4645..4974
                     /locus_tag="VG709_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32571.1"
                     /translation="MVSVARETLLTAEVAAAGAAPARYVTLRLAEAEERANSIIQTFG
                     SVQPPAPSSDRLRAALLDALDDVGGRVERLRIAAYRGEHAALTRLARPLREAVARLNR
                     FIPPALR"
     gene            4971..>5885
                     /locus_tag="VG709_05530"
     CDS             4971..>5885
                     /locus_tag="VG709_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWC32572.1"
                     /translation="MKKILTVALGILTAIGGFMDIGDLVTDALIGARFGLALVWVTVV
                     AVVGIMVYSEMAGRVAAATARTVFDLVRERLGARVALANLAASYFVNALTLVAELCGV
                     ALAIELVTDVHYLLWIPLVTFMAFLVIWKMPFKRMERIYGVVGLSLFAFVVAVWQLGP
                     DWGLMLHDATHPRIPPGDGVPQYLFYAIVMLGAQMTPYEVFFFSSGVVEHHWTPKNLT
                     EIRMNNLLGYPIGGVLAIGIQAAAYLVLRPGGIEVTHLSQTVLPVAVALGKVGFAFAV
                     VGIVAATFGAVIEPLLSAGYTVAHHFGWS"
BASE COUNT          879 a         2046 c         2096 g          864 t
ORIGIN      
        1 gccgagcacc gcccagacga acgggctgtg cccctgggtg tcgagccacg ggtggaagtg
       61 gtccgagatc agtgcgaacg ggaagccgac ctcttcggcg cgacgggcga ggcgaacgag
      121 atcggtcggc ggctgctcct cgctggacag cgtgtacccg atctccggca cgtcgcccta
      181 tctacccggc ggcctccgcg gcgaaccgct cgccgttccg tcagcatggc gcggtgccga
      241 cgaacctcga gatcgcggag ctcctcgctc gcgagtccga ggtggccgac acggaccgac
      301 gtcggcgggc gctgcgacgc gcctcgcgcc atgcgctcgg ctgggagcag gaggccgcgg
      361 acgtcgtcgc atccggcgca cccatcacga cgctctccgc cgtcgggccg tggatcgcgg
      421 gcaagatccg cgcgtggctg gacgaccctc cggagcggcc cgacccaccg cccgagcgcg
      481 ccggattcat gacgcgcgcc gaggcgcgcc gcatcctcgc gagcagcccc gactggagca
      541 gccctcgcgc cgatctccag gtccatacga cgtggagcga cgggcacgcg tcgctcgacg
      601 agatggtccg cgtcgcttcc acgtacgggt atgaccacgt ggcgatcacc gaccactcga
      661 agggccttcc gatcgcgaac ggcatggatg aaccgacgct cgccgagcag ggccgtgaga
      721 tcgcacgcct gaaccgcacg ctcgacacgg ccggcgcacc catccgcgcg ttgcgctcgc
      781 tcgagatgaa cctctcgccg gagggcgacg gcgacatgga ggcggacgcc ctcgagcggt
      841 tggatctcgt cctcggcgcc ttccactcga agctccgcgt tcgcgaggat cagacccggc
      901 gatacgtcgc cgcactccgc aacccacgtg tccacgtcct cgcacacccc cgcgggcggc
      961 gctggagcac gcgcgcaggt ctgtcggcgg attgggagac cgtcgtgacg gcggcgtccg
     1021 agacacggac ggcgctcgag atcgacgcct accccgaccg tcaggacctg gacgtcgcga
     1081 ccctccgact cgccgcggaa gcgcaagcgt ggatctcgat cgggaccgac gcgcactatc
     1141 cggacgagct gcgcttcctc gagctcgggc tcgcggcggc ggtcgaggcg ggcatacgcc
     1201 gagaacgcgt cttgaacttc cttccgcgcg acgacctgct cgcgtgggcg cgggaacgct
     1261 gaccgtttgc ccgtagggcc cctcgggtac tgacacagag gaagcaagca ctccgacgga
     1321 gggatcgtca tggccgtgaa aacgaaggct cgtgaagctc gcgagaagac cctggagacg
     1381 ttcgggaccg ctgcggagcg cgggaaggac gtcctcggga cggccgccgg ccgcgggaag
     1441 gacgtcctcg ggacggccgc cgagcgcggc aaggacgtcc tagggagcgc gaccgagcgc
     1501 gcgagggagt acacgccgaa gcgaatccgc gcgcgccgcg agaagcagcg gcggcgcgag
     1561 cggcggcgga gcctcctgaa ggggctcggc gtcgggctcg ccgtcgccta cttcttcgat
     1621 ccggacaacg gcaaccgccg acgcaacatc gcccgcgacc gcgtgatggc gctgttccgc
     1681 acgggagccg accgcggcgc gcggctgggg cgctatgcgg cgtcgcagac gtacggcgcc
     1741 gtgcagaagg tccggcacgc cggccccgac gcaccgcccg agaacgacgc gacgctcgct
     1801 cgcaaggtcg agagcgaggt cttgggcaac tctccgctca agggcaacgt cagcgtgaat
     1861 gccgagcgcg gcgtcgtcgt tctccgcggg cagatcgagc gacccgacga catccgcgac
     1921 ctcgaggacc gcgtccggaa ggtgagcggc gtgcaggacg tccgcaacct cctccacacg
     1981 ccgggtgcgc cgacccccgg gcgtaccacg acggggtagg cgttgccgct cgaacagacg
     2041 cggatcgacc gcgaggccac ctctgaggag acgttccttc agatcctcga cgaggcgacc
     2101 gcggtcctcg accggctcga agtgccctac ggcttgatcg gcgggatcgc gtcggcggtc
     2161 gagggccgcc cccggtggac gaaccgaggc gaggacatcg acgtcttcgt gcgccacaag
     2221 gacgcgatgc gtgcgctcga ggcgctcgcc gaggcggggt tcgagaccga ggagacgaac
     2281 gagaactgga tctacaaggg cgcgaaggac ggcgtcctcg tcgacgtgat cttccgctcg
     2341 accggcgaca tatacctcga cgacgagatg gcggcgcgga tccaggccgg cgagttccga
     2401 gggcgcgtcg tgaagctgat ccctcccgag gacgtcctca tcatgaaggc ggtcgcgcac
     2461 ggcgaggaga cgccgcagta ctggcacgat gcgctctcgg tgctcgcgcg cgcggacatc
     2521 gactggaact atctgctgcg gcgggcccgg cagcacggcg cgaggcgggt gctcagcctc
     2581 ctgatctacg cgcagtcgaa cgacctcgtc gtaccgccgg aaatcgtcgc ggagctgttc
     2641 gacatggtcc agccgcgctg aggaggctcg cgatggagaa ccccgaacgc gcggcgcgcg
     2701 aagggctcat cgcccaggtg cgcgaggccc ttgcggagga cccgcgcgtg ggcgagatgg
     2761 aggtgaaggt ggagctccac ggagagcgcc tggtgatcct cggcaccgtg gcgacgccgg
     2821 cgcggcgcga gcggatctcg cggatcatcg cggagcggtt cccgggacac gacatcgaca
     2881 accggacgga ggtcccgccg ctcgcggagc ccgaggaggc cgagcggctg tcatgatccg
     2941 cgtcgccgcc gtcggggacg tgcacttcgg gccggactca cgcggcaact accggccgag
     3001 cctctcgcgc ctgtcggagc gggccgacgt cttccttatc gccggagacc tgacgcgctg
     3061 gggagacccg gaggaggcac gggcgctcgc cgaagagctc catgagctcc cggtgccgac
     3121 gttcgccgtc ctcgggaacc acgactacca gtccgggcgc gaggacgacg tggtcgacgt
     3181 gatggcggcg tgcgggatcc gcgtcctcga cggcgacggc gtgacggtcg aggtcggcgg
     3241 cgtgcgcgtc ggcatcgcgg ggacggccgg cttcggtggc gggttcgccg gcggccacgc
     3301 gacggacttc ggcgagcccg agacgaaggc gttcgtggga aggaccatcc gaatggccgg
     3361 cgagctcgag ggcgcgctgc tcgacctcga cgccgacgtg cgaatcgcgc tgctccatta
     3421 ctcgccggtc cgggagacgc tctccggcga gcccccggag atccatgcgt tcctcgggag
     3481 ctacctgctc gccgaggcta tcgaccgcgc gggcgccgac ctcgcgctcc acgggcacgc
     3541 gcaccgaggg accgagaagg gagcgacgcc gagaggcgtg cacgtccgca acgtcgcctg
     3601 gccggtcatc gagaaggcat acgcgctgta cgagctcgag ccgcgcaagg cgcggcgcga
     3661 gcagctcgtg cgctaggcgc tacgacttct tctggagcat ctgcttgatc tcgtcgatct
     3721 tgcgctccat gcggtcggtg ctgtccttcg actcggcgct cccgtggtgg atcatcatcg
     3781 cggcgagcac gacgaacgag aacagctgca ggaactccga ctgccagttc tcgagcgtcg
     3841 actggctgaa gtgggcgatg aactgcccga gctcggtcgg ctcgttgtgc gccctctgct
     3901 cgtcgacgaa ctgcacccac tgcacgagcc cctgcgcgag ccacgtcgtg aggaacagga
     3961 tcatgagcgt cagcccgagc ccgaagttgt cccagatcct ccggacgccc gttcgttgcc
     4021 gctccgtgta gatgtgacgg aagagcgtcg cgacgaggaa gatcacgacg gcgcccgtga
     4081 ggagcgcgcc gatcccgagc aggaccgtgt ccatgccctc tcctcccttc cgaggtgctt
     4141 gccttgtacc ccgggagaag cggttctagt cccgcctagg cgccttcggg ggttgcggcg
     4201 tccgggctcg agtcggcggc gtcgtccgga atgccgcccg tcggctcggg gggcgtccgg
     4261 tcctcccctg cctcgtcggg gtcgggctcc gggcgcgctc cttctgagtc gccggggccc
     4321 ggcgcctccg gctcgacctc gtcggcgtcg acggtcggat ccttctccgg cgaacggtcc
     4381 ggtcgctctt ccatgacacg gctctacccg tgaccggaag ggcctacgca ccaggaggcc
     4441 gccgctcgag ggcgtaagtc cggctcgctt cgggaatcac gagcgcggat gtcactgcgg
     4501 cgatcgcgcg gacggtgtgg tcgtggccgg gcttcccgcc atgtcggggt cgtcgtcgcc
     4561 acgctcgtgc ttctcccgtc gtgcgtcgcg ccggcgctca cggccgccga ctaccgcgac
     4621 gacgctatcc gtaccgcaga ggcgatggtc tccgtcgccc gggagacgct cctcacggcc
     4681 gaggtcgccg ccgccggggc ggcccccgcg cgctacgtca cgcttcggct ggccgaggcc
     4741 gaggagaggg cgaacagcat catccagacc ttcgggagcg tgcagccgcc ggcgccgtcg
     4801 tccgatcgcc tccgcgccgc gctcctcgac gcgctcgacg acgtaggcgg gcgggtcgag
     4861 aggctcagga tcgccgcgta tcgaggagag cacgcggcgc tcacgcggct cgcgagaccg
     4921 ctccgagagg cggtcgctcg tctcaaccgc ttcatcccgc cggcgctccg atgaagaaga
     4981 tcctgaccgt agcgctcggc atcctcaccg cgatcggcgg cttcatggac atcggcgacc
     5041 tggtcaccga tgcgctgatc ggcgcgaggt tcgggctcgc gctcgtgtgg gtgacggtcg
     5101 tggcggtcgt cggcatcatg gtgtactcgg agatggcggg acgcgtggcc gccgcgacgg
     5161 cgcgcacggt cttcgatctc gtgcgggagc gtctgggcgc gcgcgtcgct ctggcgaacc
     5221 tcgccgcgtc gtacttcgtc aacgcgctca cgctcgtcgc ggagctgtgc ggggtagcgc
     5281 tcgccatcga gctcgtcacc gacgtgcact acctgctgtg gatcccgctc gtcaccttca
     5341 tggcgttcct cgtgatctgg aagatgccgt tcaagcgcat ggagcgcatc tacggcgtcg
     5401 tcggcctgtc gctgttcgcc ttcgtcgtcg cggtatggca gctcggcccc gactggggcc
     5461 tgatgctcca cgacgcgacg catccgcgga tccctccggg ggacggcgtc ccgcagtacc
     5521 tcttctacgc gatcgtcatg ctcggtgccc agatgacgcc gtacgaggtg ttcttcttct
     5581 cgtcgggcgt cgtcgagcac cactggacgc cgaagaacct caccgagatc cgcatgaaca
     5641 acctcctcgg atacccgatc gggggcgtcc tcgcgatcgg catccaggcg gcggcctacc
     5701 tggtgctgcg gcccggaggg atcgaggtga cccacctcag ccagacggtg ctcccggtgg
     5761 ccgtcgccct cgggaaggtc gggttcgcct tcgccgtcgt gggtatcgtc gccgcgacgt
     5821 tcggcgcggt gatcgagccg ctgctctcgg cggggtacac ggtggcgcac catttcgggt
     5881 ggtca
//
LOCUS       DASZOB010000344         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_57778, whole genome shotgun sequence.
ACCESSION   DASZOB010000344 DASZOB010000000
VERSION     DASZOB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57778"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VG709_05535"
     CDS             complement(<1..625)
                     /locus_tag="VG709_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32573.1"
                     /translation="MPGRDLLALSGVAAGYHGRPAVIDVTLAVREGEVVGLIGPNGSG
                     KTTLVRVASRVLRPFAGRVEVAGRDLFAMSARDAARLVAVVPQETAPVFAFTALEIVL
                     MGRSPYLPAWGSGSPRDFRRAREAMALAGVDELAERTIDELSGGEKQRVVLAQALAQD
                     APVLLLDAPTTHLDPGHILGVLAAVRELARGGRAVLSVFHDLNLAAAS"
     gene            complement(612..1640)
                     /locus_tag="VG709_05540"
     CDS             complement(612..1640)
                     /locus_tag="VG709_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWC32574.1"
                     /translation="MSRRGVALPVLATLLVAVVLVAIGLGTVWISPATTLRLIASRLG
                     IADQPDTPGSLAVILFELRPPRVMLAILVGAALAVAGPVFQAVFRNPMADPAIIGVSS
                     GAALGAVVAILVTGSAFAGGLAVPLAAFAGAMATALLVYRIARIGPTVQVATLLLGGI
                     AVAAVISAIITLVMSLSGEEIRSIYFWLLGGLAARGWNTLWAAAPLVAVGIVAALAAV
                     RDLNVMALGEERAAQLGVEIERFKRLALATGALLAGAAVSVAGVIGFVGLMTPHILRL
                     VLGADHRRLLPATVLGGALFMVLADLAARTLLAPEEIPVGAVTALLGGPFFLYLLRRE
                     RRVAGARA"
     gene            complement(1637..2548)
                     /locus_tag="VG709_05545"
     CDS             complement(1637..2548)
                     /locus_tag="VG709_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HWC32575.1"
                     /translation="MSRIARLAAIASVFVLLGCGAGQDPRVAEAPTSPAAAFPVTITD
                     DEGVEVTIEEPPERIVTFAPSHTEIVFALGAGERLVGVSGAFDDYPPEASDLPHVAGP
                     SGTQPNIEKVVSLEAQVVLTAFIGGEWKDRLRGLQVPVVTTLPDSLDDALGDMETIGR
                     VIGAEAEATALTEELRAEVEAIEENVGDDRVTCFLELSDLFTVGPGSLEYDLLERAGC
                     DPVTSSADQPYPQWSVEQLVSDDPDVFLASETGAPVDEIVDRPGIRDLTAVKDGRVAV
                     VSGDLISRPGPRVVEGLRELADALHAG"
     regulatory      complement(2660..2787)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2892..>3182)
                     /locus_tag="VG709_05550"
     CDS             complement(2892..>3182)
                     /locus_tag="VG709_05550"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWC32576.1"
                     /translation="GREAVARRKAEGPHERLRGLRMAGKLIPRAHYDVLADGRRVGET
                     TSGTFSPTLRIGIAMAYLEADVQPGATVEVDVRGRRGEAEVVKPPFLDRSPK"
BASE COUNT          474 a         1223 c         1100 g          385 t
ORIGIN      
        1 agctcgcggc ggcgaggttc aggtcgtgga acaccgagag caccgcgcgc ccgccccgcg
       61 cgagctcgcg gaccgccgcg aggacaccga ggatgtgccc cggatcgagg tgcgtcgtcg
      121 gcgcgtcgag gagcaggacc ggtgcgtcct gcgcgagcgc ctgcgcgagc accacccgct
      181 gcttctcgcc gccggacagc tcgtcgatcg tgcgctccgc gagctcgtcg accccggcga
      241 gcgccatggc ctccctcgcg cgccggaagt cgcgggggga gccgctcccc cacgccggga
      301 ggtacgggga gcggcccatc agcacgatct cgagcgccgt gaacgcgaag accggcgccg
      361 tctcctgcgg cacaaccgcg acgagcctcg cagcgtcgcg cgccgacatc gcgaacaggt
      421 ctcgtccggc gacctcgacg cggcccgcga acggccggag gacgcgcgac gcgacgcgca
      481 cgagcgtcgt cttcccggag ccgttcggcc cgatcaaccc gacgacctct ccctcccgta
      541 ccgcgagagt cacgtcgatc accgccggac gaccgtggta cccggccgcg acgccggaga
      601 gcgcgaggag gtcacgcccg ggcaccggcc accctccgct ctcgacggag cagatagagg
      661 aagaacgggc caccgagcag cgccgtaacg gcgccgacgg ggatctcctc gggcgcgagg
      721 agcgtcctcg ccgcgaggtc ggccaggacc atgaacagcg cgccgccgag cactgtggcc
      781 gggagcagcc gccggtgatc cgcgccgagc acgagccgca ggatgtgcgg cgtcatcagc
      841 ccgacgaacc cgatcacccc cgcgaccgag accgcggcac cggcgaggag cgcgccggtc
      901 gcgagcgcca gccgcttgaa acgctcgatc tcgaccccca gctgcgccgc ccgctcctca
      961 ccgagcgcca tcacgttgag gtcgcggacc gccgcgagcg ctgccacgat cccgaccgcg
     1021 acgagcggag ccgccgccca cagcgtgttc catccgcgcg ccgcgagccc gccgaggagc
     1081 cagaagtaga tcgagcggat ctcctctccg gacagcgaca tcacgagcgt gatgatcgcc
     1141 gagatcacag ctgcgaccgc gatccctccg aggagcagcg tcgcgacctg cacggtcggg
     1201 ccgatccgcg cgatgcggta tacgagaagc gccgtcgcca tcgcgccggc gaatgcggcg
     1261 agcggaaccg cgaggccgcc ggcgaaggcg ctccccgtga ccaggatcgc gacgaccgcg
     1321 ccgagcgccg ccccggagga gacgccgatg atcgccgggt cggccatcgg gtttcggaag
     1381 accgcctgga agaccggccc ggcgaccgcg agcgccgccc ccacgaggat cgcgagcatc
     1441 acgcgtggcg gcctcagctc gaacaggatc acggctagcg atcccggcgt gtccggctga
     1501 tcggcgatcc cgaggcgcga ggcgatgagc cggagcgtcg tcgccgggga gatccacacg
     1561 gtgccgagcc cgatcgcgac gagaaccacg gcgacgagca gcgtcgcgag gaccggcagc
     1621 gcgacgccgc ggcggctcac ccggcgtgga gcgcgtcggc gagctcgcgc agcccctcga
     1681 cgacgcgcgg tcccggccgt gagatcaggt cgccgctcac gacggccacg cgaccgtcct
     1741 tcacggccgt gagatcgcgg atccccggac ggtcgacgat ctcgtcgacc ggcgccccgg
     1801 tctccgacgc gaggaacacg tcggggtcgt cgctgaccag ctgctcgacc gaccactgcg
     1861 ggtacggctg gtccgccgac gacgtcacgg ggtcacagcc cgccctctcg agcaggtcgt
     1921 actcgaggga tccaggaccg acggtgaaca ggtcggagag ctcgaggaag cacgtgacgc
     1981 ggtcgtcccc gacgttctcc tcgatcgctt cgacctcggc gcggagctcc tcggtcagcg
     2041 ccgtcgcctc ggcctccgct cctatcacgc gcccgatcgt ctccatgtcg ccgagcgcgt
     2101 catcgaggct gtcgggaagc gtcgtgacga cgggtacctg gagtcctcgc agccggtcct
     2161 tccactcgcc gccgatgaac gccgtcagca cgacctgtgc ctcgagcgac acgaccttct
     2221 cgatattggg ctgcgtaccg gacggcccgg cgacgtgggg aaggtcgctc gcctcgggcg
     2281 ggtagtcgtc gaacgcgccc gagacgccga cgagacgctc gcccgcaccg agcgcgaaca
     2341 cgatctcggt gtgcgacggg gcgaacgtca cgatccgttc ggggggttcc tcgatcgtca
     2401 cctcgacgcc ctcgtcgtcg gtgatcgtga ccgggaacgc cgccgcgggc gacgtcggcg
     2461 cctcagcgac gcgcgggtcc tgtccggcgc cgcatccgag gagcacgaag acgctcgcga
     2521 tcgcggcgag tcgcgcgatc cgcgacatgg gccctccttc ctggccgaac gcgcggccgt
     2581 ccgggccgtc ccacggaacg cggagcgcct cgaggcccgc cgccttcccg cgagggacgg
     2641 cgccttccct ccccgaagca ggtctcctgg ctcccggatc gcggctcccc cgagccttcc
     2701 caggaggcga acctcccagt ggcgtccacg gggtcgctcc ccggtcacag tggcgcgacc
     2761 gcgccggact ctcaccggct tccctctccc aggcctctcg acccggtcgg ggcgggcgtt
     2821 gccgcccgcg ccacctcggg gccttctctg cgtgattgtc gctcgcgcgc cgcacgcgcg
     2881 gcaagcgccg gctacttcgg cgagcggtcg aggaacggtg gcttgacgac ctcggcctcg
     2941 ccgcggcgcc cccgaacgtc cacctccacg gtcgcgccgg gctgtacgtc ggcctcgaga
     3001 taggccatcg cgatcccgat ccgaagcgtg ggagagaacg tcccgctcgt cgtctcgccc
     3061 acacgccgac catccgcgag gacgtcgtag tgcgcccgag ggatcagctt cccggccatg
     3121 cggagcccgc ggagccgctc gtgcggccct tcggctttcc tgcgcgcgac cgcctcccgc
     3181 cc
//
LOCUS       DASZOB010000345         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98156, whole genome shotgun sequence.
ACCESSION   DASZOB010000345 DASZOB010000000
VERSION     DASZOB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98156"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1641
                     /locus_tag="VG709_05555"
     CDS             <1..>1641
                     /locus_tag="VG709_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC32577.1"
                     /translation="EWFWDAVVQDLDIELTTPYERVLDISDGVPWARWFVGGKTNLAH
                     NCVDKWAQRTPDAAAVLWEGEDGVTRRVTYRELREMADRLAGGLRSLGVGERDAVGIF
                     MPMSPETVAATLACAKLGAINLPIFSGYAADAVATRLRDAEAKFLVTADGFHRRGQPF
                     RMKEVADEAADASPSVERVVVWSRLDRDDLPWNERRDVRWDELLAAQAPRFETLPLDS
                     EHPLFVAYTSGTTGRPKGSVHVHGGFQVKIAEEVAYQADLHQDETLFWVTDLGWIMGP
                     WEIVGAGALGATVFLYEGAPNHPAPDRIWDIVERHRITTLGVSPTLIRALIPAGDEHV
                     ERHDLSSLRILASTGEPWNPEPYLWFFEKVGGGRCPIINISGGTEVGACFLSPLPITD
                     LKPTTLRGPALGMAVDVWGPDGKPVPRGQVGELVCTKPWPAMTRGVWRDRDRYLETYW
                     SRWPDAWVHGAWATIDEDGFWFIQGRSDDTIKIAGKRLGPAEVESAAVAHPAVQEAAA
                     IGVPHEVKGDAVVVFAVLRPGHEPGERLRQEIEGRIVA"
BASE COUNT          235 a          569 c          600 g          237 t
ORIGIN      
        1 tcgagtggtt ctgggacgcg gtcgtgcagg acctggacat cgagttaacc acgccctacg
       61 agcgcgtcct cgacatctcc gacggtgtgc cctgggctag gtggttcgtc ggcgggaaga
      121 cgaacctcgc gcacaactgc gtcgacaagt gggcccagcg gactcccgac gcggccgcgg
      181 tgctgtggga gggagaggac ggcgtcacac gccgggtcac gtaccgcgag ctccgggaga
      241 tggccgaccg cctcgccggc ggcctccgtt cgctcggcgt cggcgagcgc gacgccgtcg
      301 gcatcttcat gccgatgtcg cccgagaccg tcgccgcgac gctggcttgc gcgaagctcg
      361 gcgcgatcaa cctgccgatc ttttcggggt acgcggccga cgcggtcgcc actcggctcc
      421 gcgacgcaga ggcgaagttt ctcgtcacgg ccgacgggtt ccaccgacgc gggcagccgt
      481 tccgcatgaa ggaggtcgcc gacgaggcgg cggacgcgtc gccgtcggtc gagcgggtcg
      541 tggtctggtc gcgtctcgac cgtgacgacc tgccgtggaa cgaacggcgc gacgtccggt
      601 gggacgagct cctggccgcg caggcgccga ggttcgagac gctcccgctg gactccgagc
      661 atccgctgtt cgtcgcgtac acgagcggca cgaccggtcg cccgaagggc tcggtccacg
      721 tccacggcgg cttccaggtg aagatcgcgg aggaggtcgc gtaccaggcc gacctacacc
      781 aggacgagac gctgttctgg gtcaccgacc tcgggtggat catgggcccc tgggagatcg
      841 tcggcgccgg cgcgctcggg gcgacggtgt tcctatacga gggcgcgccg aaccatccgg
      901 cacccgaccg gatctgggac atcgtcgagc gccaccggat cacgacgctc ggcgtctcgc
      961 ccacgctcat ccgcgcgctt atcccggccg gcgacgagca cgtcgagcgg cacgacctct
     1021 cctcgctccg gatcctcgcc tcgaccggcg agccgtggaa ccccgagccg tacctctggt
     1081 tcttcgagaa ggtcggcggt ggccgctgcc cgatcatcaa catctccggc ggcacggagg
     1141 tcggcgcgtg cttcctctca ccacttccga tcacggacct gaagccgacg acgctccggg
     1201 gccccgcgct cgggatggcg gtcgacgtct ggggccccga cgggaagccc gtccctcgag
     1261 gccaggtcgg cgagctcgtg tgcacgaaac cttggcccgc catgacgcga ggggtctggc
     1321 gcgaccgcga ccgctacctc gagacgtact ggtcgcgctg gcccgacgca tgggtccacg
     1381 gcgcctgggc gacgatcgac gaggacggct tctggttcat ccaggggcgc tcggacgaca
     1441 cgatcaagat cgccggcaag cggctcggcc cggccgaggt cgagtcggcg gcggtggcgc
     1501 accccgcggt gcaggaggcg gcggcgatcg gcgtgcccca cgaggtgaag ggggacgcgg
     1561 tcgtcgtctt cgcggtgctg cgccccggcc acgagccggg cgagcggttg cggcaggaga
     1621 tcgaggggcg gatcgtcgcg c
//
LOCUS       DASZOB010000346         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_27701, whole genome shotgun sequence.
ACCESSION   DASZOB010000346 DASZOB010000000
VERSION     DASZOB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27701"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VG709_05560"
     CDS             complement(<1..756)
                     /locus_tag="VG709_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32578.1"
                     /translation="MVAEVQANGGVAVGRRPRAFVRVAGPQAEDYLQRIVSNDVAALE
                     PGAAQEALLLTPKARVIAPLTIVRRAADDFLLLTEPELGETVRDHLLRFRFAARVAIE
                     LEDHASYLLLGASAAAGAESLAVPNADYGVPAVEVIDESPPEHAREVGEEELERLRIE
                     AGTPRFGREIDDRVLPAEAGLVERAVSLTKGCYPGQEPIARLHYRGHANRGLRVLEVE
                     GDELPEYDAEVRLGEKVVGRVTSAARTGKGTVLA"
     gene            837..>1626
                     /locus_tag="VG709_05565"
     CDS             837..>1626
                     /locus_tag="VG709_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32579.1"
                     /translation="MQRVSREGVEFSPDRPQHEHVGVVMEGVSRARVAKLASVIGLVA
                     CLAAPATAAASEKIGANASNVSLKVDANGRAVVYYTSAGKRYHPVVWGAVNARRPSAT
                     VPQVTFKIDYSGGYMKLGYPLWKTIKNRCRPYDGPRLPWFVAGCKAPDGSYWALQRFQ
                     RLLPNLGLNPWLPRHRAKELHVSHFTGAPAKLDVYADWVMSSRTHEVFGQLTYRGNAV
                     HGIKASPSGVPLDGYGRLIYLDVFNAVDPAYPRGWSRENSFLARK"
BASE COUNT          244 a          584 c          564 g          234 t
ORIGIN      
        1 cgccagcacc gtgcctttcc ccgtccgagc ggcgctggtg acgcggccga cgaccttctc
       61 gcccagacgc acctcggcgt cgtactcggg aagctcgtct ccctccacct cgagcacgcg
      121 caggccgcgg ttcgcgtggc cgcggtagtg gaggcgggcg atcggctcct ggcccgggta
      181 gcagcccttg gtcaggctga cggcgcgctc gacgaggccg gcctcggccg ggaggacgcg
      241 gtcgtcgatc tcgcggccga accgcggcgt cccggcctcg atcctgagcc gctcgagctc
      301 ctcctcgccg acctcgcgcg catgctcggg gggagactcg tcgatgacct cgacggccgg
      361 aacgccgtag tcggcgttgg gaaccgcgag ggactccgct ccggccgccg cggaagcgcc
      421 gagaagaagg taggacgcgt ggtcctcgag ctcgatcgcg acacgcgcgg cgaagcgaaa
      481 gcggagaagg tggtcgcgca cggtttcgcc gagctcgggc tccgtcagga gcaggaagtc
      541 gtcggcggcg cgccggacga tggtgagcgg cgcgatcacg cgcgccttcg gcgtgaggag
      601 gagcgcttcc tgcgcagctc cgggctcgag cgccgcgacg tcgttcgaca cgatccgctg
      661 caggtagtcc tccgcctgcg gccccgcgac acgcacgaac gcccggggcc gacggccgac
      721 cgcgactccg ccgttcgcct ggacctcggc taccacgccg accgctccga cgacggagcc
      781 gcggaagcct caagttcttg ccggttcagc cgatacatcg tcacgtgggg ggtctcttgc
      841 aaagggtatc ccgcgaaggg gtcgagttct cgccggaccg gccacaacac gaacacgtgg
      901 gggttgtgat ggagggagta tcccgggcgc gcgtcgcgaa gctcgcctcg gtcatcgggc
      961 tcgtcgcctg cctcgccgcg ccggcgacgg cagccgcgag cgagaagatc ggcgcgaacg
     1021 cgtcgaacgt cagcctgaag gtcgacgcga acggacgtgc ggtcgtttac tacacgagcg
     1081 cggggaagcg ctaccacccc gtcgtctggg gcgccgtgaa cgcgcgccgg ccctccgcga
     1141 ccgtgccgca ggtcaccttc aagattgact actcgggcgg ctacatgaag ctcggctacc
     1201 cgctctggaa gacgatcaag aaccgctgcc gcccgtacga cgggccgcgg ctgccgtggt
     1261 tcgtggccgg atgcaaggcg cctgacggaa gctactgggc gctccagcgc ttccagcgcc
     1321 tcctcccgaa cctcggcctc aacccgtggt tgcccaggca tcgtgccaag gagctccacg
     1381 tctcgcactt cacgggcgcg ccggcgaagc tcgacgtcta cgccgactgg gtgatgagct
     1441 cgcgaacgca cgaggtgttc ggtcagctga cgtaccgcgg gaacgccgtc cacggcatca
     1501 aggcctcgcc ctcgggcgta ccgctcgacg ggtacggccg cctcatctac ctcgacgtct
     1561 tcaacgccgt cgaccccgcg tacccgcgcg gctggtcgcg cgagaactcg ttcctcgcgc
     1621 gcaagc
//
LOCUS       DASZOB010000347         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128270, whole genome shotgun sequence.
ACCESSION   DASZOB010000347 DASZOB010000000
VERSION     DASZOB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128270"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VG709_05570"
     CDS             complement(<1..1260)
                     /locus_tag="VG709_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWC32580.1"
                     /translation="MLTTIAALLVPLAINLRARAEAELKAQALVQAQTVASAIGDARV
                     RDWQRIVTRAQRRATGGRVIVVDGSGILVADSAGRELLGQRYATDDRPELVAAVDGVS
                     RSLVRRSETLGQDILATAVPIPPDRGAVRVTLPMDEVRRNAGRTLLGLVAIGLAGLAA
                     GLLIAFIVAGTLSRPLQRVARTAERLGAGELGVRTGKVRGPQEIEEVAREFDEMASRL
                     EATVRGQREFAANASHQLRTPLTGLRLRLESAAEDASPELRRELEAAQHEVDRLAAIV
                     ERLLVLAKRRESDAGRAVPLRPAADRAVERWSVRAREAGATLEARGDGGEALADPGDV
                     DQILDSLIDNGISHARGPIVVEAGRMDGVSFVAVEDRGPGIPRAERGRVVERFYRGRG
                     ASAGGSGLGLAIVRELAERWGGSVEIGP"
     gene            complement(1306..2007)
                     /locus_tag="VG709_05575"
     CDS             complement(1306..2007)
                     /locus_tag="VG709_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC32581.1"
                     /translation="MATRVLVVEDEEAISQPLARSLEREGFEPEVAPTVADAEDAFGR
                     RTPDVVLLDLMLPDGDGRDLCRSFRSRSDVPIIMLTARGEEVERVVGLELGADDYVVK
                     PFSARELAARIRAVLRRAAAPATQRDETIEGGDIRLDPRSRTVTKRGDAVELAAREFD
                     LLHMLMRTPGAVVRREEIMDEVWDPHWFGSTKTLDVHISWLRKKLEDDPATPRYITTV
                     RGVGFRFATDDDLGG"
     gene            2199..3341
                     /locus_tag="VG709_05580"
     CDS             2199..3341
                     /locus_tag="VG709_05580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC32582.1"
                     /translation="MAHTLAARGITTPFPIQLLVMRDALAGHDVLAKSRTGSGKTLAF
                     AAPIVERVRAGDRKPSALVLVPTRELASQVVGELADIAATKRLSVAAAYGGVSIREQS
                     KAIARADVLVATPGRLEDLATRRLVNLGAVRILILDEADRMLDMGFEPQVAALVRRLP
                     RERQTMLFSATLDGGVAGLAARYTRDAVRHEVETSRPTVDEADHRFVPVSAAAKVETL
                     VDLLRAEPGRALVFVRTKRGSDRLVARLRRHGVAAVAMHGDISQTAREHALRRFHRGE
                     VTTLVATDVAARGLDVDDIAHVIYYDPPADDKDYVHRVGRTARAGKTGTGVTLVLPEQ
                     QSDVSRMAARLRLAAEFTAEGMTVAPPRTVFTSRGRRSGMTRRRAS"
     gene            3407..3856
                     /locus_tag="VG709_05585"
     CDS             3407..3856
                     /locus_tag="VG709_05585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWC32583.1"
                     /translation="METSRGEIAFELFADRTPATVNNFVFLARNGFYDGVPFHRVIEG
                     FMLQGGDPTGTGRGGPGYTFDDEIVDDLRFDQPGLLAMANAGPATNGSQFFVTVSSPR
                     HLDGRHTIFGRVVEGYEVVEDISRMSTDSADRPLEDAVIERVRIDED"
BASE COUNT          564 a         1470 c         1403 g          570 t
ORIGIN      
        1 cggcccgatc tcgacgctgc cgccccagcg ctcggccagc tcgcgcacga tcgccagccc
       61 gagtcccgaa ccacccgcac tggcgccgcg cccgcggtag aagcgctcga cgacgcgacc
      121 ccgctccgcg cgagggatcc ccggcccgcg gtcctcgacg gcgacgaacg agacgccgtc
      181 catgcgtccg gcttcgacga cgatcgggcc gcgggcgtgc gagatcccgt tgtcgatcag
      241 gctgtcgagg atctggtcga cgtcgcccgg atcggcgagc gcctcgccgc cgtcgccgcg
      301 ggcctcgagc gtcgcgccgg cctcgcgcgc ccggacgctc cagcgctcga cggctcggtc
      361 cgccgccggc cgcagcggaa cggccctccc ggcgtcggac tcgcgccgct tcgcgagcac
      421 gagcagccgc tcgacgatcg ccgcgaggcg gtcgacctcg tgctgagcgg cctccagctc
      481 gcggcgcagc tcgggggacg cgtcctccgc cgccgactcg agccggagcc gcagcccggt
      541 cagcggcgtc cgaagctggt gcgatgcgtt tgcggcgaac tcccgctggc cgcgaaccgt
      601 cgcctcgagc cgcgacgcca tctcgtcgaa ctcgcgagcg acctcctcga tctcctgcgg
      661 gccgcgcacc tttccggtcc gtacgccgag ctcgccggct ccgagccgct ccgcagtgcg
      721 cgcgacgcgc tggagcggac gcgagagcgt cccggcgacg atgaacgcga tgaggagccc
      781 tgctgcgagc ccggcgagac cgatcgcgac gaggcccagg agcgttcggc cggcattccg
      841 cctgacctca tccatcggaa gcgtgacgcg caccgcgccg cggtcgggcg ggatcggcac
      901 ggccgtcgcg aggatgtcct gtcctagcgt ctcgctgcgg cgaacgaggg agcgagagac
      961 gccgtccacc gccgcgacca gctcgggacg atcgtcggtc gcgtagcgct gtccgagcaa
     1021 ctcgcggccc gcggagtcgg cgacgaggat gccggagccg tcaacgacga tgactcgccc
     1081 acccgtcgcg cgacgctgtg ccctcgtcac gatccgctgc cagtcacgga cgcgcgcatc
     1141 gccgatcgcc gacgcgacgg tctgcgcctg cacgagcgcc tgcgccttga gctcagcctc
     1201 cgcgcgagcg cggaggttga tcgcgagcgg caccagcagc gccgcgatcg tcgtcagcaa
     1261 gatgtacgcg aacgcgagga ggagccgcga cctgagcttc gtcgcctacc ctccgaggtc
     1321 gtcgtcggtc gcgaaccgga aaccgacgcc gcggacggtc gtgatgtacc gcggggttgc
     1381 cgggtcgtct tccagcttct tgcgcagcca cgagatgtgg acgtcgaggg tcttcgtcga
     1441 gccgaaccag tgcgggtccc agacctcgtc catgatctcc tcccgtcgca cgacggctcc
     1501 cggcgtgcgc atcagcatgt ggaggaggtc gaactcccgc gccgcgagct cgacggcgtc
     1561 gcctcgcttc gtgacggttc gcgacctggg atcgaggcgg atgtcgccgc cctcgatcgt
     1621 ctcgtcccgc tgcgtcgccg gcgcggccgc gcgacggagg acggcgcgga tccgtgcggc
     1681 gagctcgcgc gccgagaacg gcttgacgac gtagtcgtcg gcgccgagct cgagtccgac
     1741 cacccgctcg acctcctcgc cgcgcgcggt cagcatgatg atcgggacgt cggaccgaga
     1801 tcggaagctg cggcagaggt cgcggccgtc gccgtccgga agcatgaggt cgagcaggac
     1861 gacgtcgggc gtcctgcgac cgaaggcgtc ttcggcgtcg gcgacggtcg gcgccacctc
     1921 cggctcgaac ccctcgcgtt cgaggctcct cgccagcggc tgcgagatcg cctcctcatc
     1981 ctcgacgacg aggacccgcg ttgccatggg ccgatagtac ggcgcctgat atcctttgag
     2041 gcacgggtga cgtccgtcac ctcgtgaggc gccacttccg cggttccctc ttcggacggt
     2101 tcggcaattt ggaagcagca cctcgccgaa ccagtcggcc tcgtgccgaa gaaggagacc
     2161 gcatgtctac gttcgaacac ctcggtgttt cgcctgccgt ggcacacacg ctcgccgcgc
     2221 gcggcatcac cacaccgttc ccgatccaac tgctcgtgat gcgcgatgcg ctcgcgggac
     2281 acgacgtcct ggcgaagtcc aggaccggat ccggaaagac cctcgcgttc gcggctccga
     2341 tcgtcgagcg cgtccgggcg ggcgaccgca agccgtcggc gctcgtgctc gtgccgactc
     2401 gcgagctcgc gtcgcaggtc gtcggggagc tcgccgatat cgccgcgacg aagcgcctgt
     2461 ccgtcgccgc cgcgtacggc ggcgtgagca tccgggagca gtcgaaggcg atcgcgcgcg
     2521 ccgacgtgct cgtcgcgacg ccgggccggc tcgaggacct cgcgacgcgc cggctcgtca
     2581 acctcggcgc ggtgcggatc ctcatcctcg acgaggccga ccggatgctc gacatgggat
     2641 tcgagccgca ggtcgccgcg ctcgtccggc ggctgcctcg cgaacgccag acgatgctgt
     2701 tctcggcgac gctggatggc ggcgtcgcgg gcctcgcggc tcgctacacg cgcgatgcgg
     2761 ttcggcatga ggtcgagacg tctcgcccga cggtcgacga ggccgaccac cggttcgttc
     2821 cggtgagcgc cgccgcgaag gtggagacgc tcgtcgatct cctccgcgcg gagccggggc
     2881 gcgcgctcgt cttcgttcgc acgaagcgcg gttccgaccg cctcgtcgcc cggctccgcc
     2941 gacacggcgt cgccgcggtc gcgatgcacg gcgacatctc gcagacagca agggagcacg
     3001 cgctccgccg gttccaccgg ggcgaggtca cgacgctcgt cgcgaccgac gtcgccgccc
     3061 gcgggctcga cgtcgacgac atcgctcacg tcatctacta cgatccgccg gcggacgaca
     3121 aggactacgt ccaccgcgtc ggacggaccg cccgggccgg caagaccggg accggcgtga
     3181 cgctcgtgct tcccgagcag cagagcgacg tgagcaggat ggcggcgcga ctgcgcctcg
     3241 ccgcggagtt caccgccgaa ggcatgaccg tcgctccgcc gcgcacggtg ttcacgtcgc
     3301 gcggacgtcg ctcgggcatg acgcgccggc gcgcgtcgtg acggcgatga ggttggatcg
     3361 cccgccggag atggagatcg acgcctcgaa acggtatcgc gcggtgatgg agacgtcgcg
     3421 gggagagatc gcgttcgagc tcttcgcgga ccgcacgccg gcgaccgtca acaacttcgt
     3481 cttcctcgca cgcaacgggt tctacgacgg cgtgccgttc caccgcgtga tcgagggctt
     3541 catgctccag ggcggggacc cgaccgggac cggtcgcggc gggcccggct acacgttcga
     3601 cgacgagatc gtcgacgacc tgcgcttcga ccagccgggc ctgctggcga tggcgaacgc
     3661 cggccctgcg acgaacgggt cccagttctt cgtcacggtg agctcccctc gccatctcga
     3721 cggccgtcac acgatcttcg gccgcgtggt cgagggctac gaggtggtcg aggacatcag
     3781 ccgcatgtcg acggactcag ccgaccgccc tctcgaagac gccgtgatcg agcgggttcg
     3841 gatcgacgag gactgaggcg gccgagcgct agtcggtcgc gccggggcgc tcgagcgtct
     3901 cggcgtcgcc cgcaggctcg ggcgcttcga actcatccgg ctcgttcgga gcgatctccg
     3961 gcccggaggg aagaacgccg gcggcccgat ccgctcgacg ctggttc
//
LOCUS       DASZOB010000348         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_77918, whole genome shotgun sequence.
ACCESSION   DASZOB010000348 DASZOB010000000
VERSION     DASZOB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77918"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VG709_05590"
     CDS             complement(<1..998)
                     /locus_tag="VG709_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWC32584.1"
                     /translation="MGTAAGASEGRRDARRARSWLGVRFRLRDDDAVDGRLAAMARAA
                     FSLYFAGGTLGLLSLAAPASAREEPAVAAASLAAYALAAFYLIGFDRVPPRVYHVTTA
                     CATGMIAFATLFGGEAALAYPLFYLWVVLYALYFFPWRQAAAHTALAAAAYVLVLALG
                     TRPLTAVQALVTGGTAFVTGGMAVLVKARLDGLLERLDRSNRLLLAHNTRLRQLDRQK
                     DELVSLVSHELKTPLTSIRGYLDLMVEDRDAFGDEHRRFLEILDRSVDRLLKVLGDLL
                     FLARVDEQTLTLALERVDLAAVVDEGVSRFTALAEQEGIALSSTVDPLPPVRADRAR"
     gene            complement(1130..>1636)
                     /locus_tag="VG709_05595"
     CDS             complement(1130..>1636)
                     /locus_tag="VG709_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HWC32585.1"
                     /translation="FVERVTEYRASVRTVAADGIADAVANVFGEAGVSRVAVPADLPP
                     AWVPANVEAVADDGLDASTLDEIGAAVTGCAFAIAETGTVVLDAGPRQGRRALTLVVD
                     LHVCVVEEDQIVDGVPTALRRLAPGLRSGRRPVTFVSGPSAPGDIEFTRIEGVHGPRR
                     LELVLVRG"
BASE COUNT          247 a          578 c          628 g          183 t
ORIGIN      
        1 cgcgcccggt cggcgcggac gggcggcagc gggtcgacgg tcgagctcag cgcgattccc
       61 tcctgctcgg cgagcgcggt gaagcggctc acgccttcgt cgacgaccgc cgcgaggtcg
      121 acccgctcga gcgcgagcgt cagcgtctgc tcgtcgaccc gggcgaggaa gaggaggtcg
      181 ccgagcacct tgagcagccg gtcgacgctc cggtcgagga tctcgaggaa gcggcggtgc
      241 tcgtctccga acgcgtcccg gtcctcgacc atgaggtcga ggtaaccgcg gatggacgtg
      301 aggggggtct tgagctcgtg cgagacgagc gagacgagct cgtccttctg gcggtcgagc
      361 tggcgcagcc gcgtgttgtg ggccagcagc agccggttcg agcggtcgag ccgctcgagg
      421 agcccatcga ggcgcgcctt gacgagcacc gccatgccgc ccgtgacgaa ggccgtcccg
      481 ccggtcacga gcgcctgcac ggcggtgagc ggccgggtgc cgagcgcgag gacgagcacg
      541 tacgcggcgg cggcgagcgc ggtgtgggcg gcggcctggc gccaggggaa gaagtagagc
      601 gcgtagagaa cgacccagag gtagaagagc gggtacgcga gcgctgcctc gccgccgaag
      661 agcgtcgcaa aggcgatcat gccggtcgcg caggccgtcg tgacgtggta cacccggggc
      721 ggaacgcggt cgaagccgat caggtagaac gccgcgagcg cgtaggcggc gagggaggct
      781 gccgcgaccg ccggctcctc gcgtgcggac gccggcgccg cgagcgacag caggccgagc
      841 gttccgcccg cgaaatacag ggagaacgcc gcgcgcgcca tcgccgcgag gcgaccgtcg
      901 acggcgtcgt cgtcgcgaag ccggaagcgc acgccgagcc acgagcgcgc gcgtcgcgcg
      961 tcccgacggc cctccgaggc gccggccgcg gtgcccatcg gggccagacg ttaccgcgag
     1021 cgggcgcgcg aagaggcccg gctgctcggc cgcgcgtctt cgcggggcgg tcgctagtgg
     1081 ttccgccgcc cttcgggttg cacacgagcg gcaccgcagg cctgcggcgt cagccgcgaa
     1141 cgagcacgag ctcgagccgc cgcggaccat gcactccctc gatccgcgtg aactcgatgt
     1201 ctccgggcgc ggacgggccg gacacgaagg tcaccgggcg gcgtccggac cgcagtccgg
     1261 gcgcgaggcg gcggagcgca gtcggtacgc cgtccacgat ctggtcctcc tcgaccacgc
     1321 agacgtggag gtcaacgacg agcgtcagcg cccgccggcc ctggcgcggg cccgcgtcga
     1381 ggacgaccgt ccctgtctct gcgatggcga acgcgcagcc cgtgaccgca gcgccgatct
     1441 cgtcgagcgt cgacgcgtcg agcccgtcgt cggcgacggc ctccacgttc gccggcaccc
     1501 acgcgggcgg cagatcggcc ggaacggcaa cccgagagac gcccgcctcg ccgaagacgt
     1561 tcgcgacggc gtccgcgatc ccgtccgccg cgaccgtacg aaccgacgcg cggtactcgg
     1621 tcacgcgctc gacgaa
//
LOCUS       DASZOB010000349         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_57848, whole genome shotgun sequence.
ACCESSION   DASZOB010000349 DASZOB010000000
VERSION     DASZOB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57848"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VG709_05600"
     CDS             <1..849
                     /locus_tag="VG709_05600"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HWC32586.1"
                     /translation="VQFAYDWMEDPWLMEATATWIEDDVFDGINDNLQFLPASPLSFG
                     APWHAIDHFHPHGSNQYGAWIFFRFLSEYFGSPTHPSSASDRTVVRDIWAAADGRGKA
                     NGGRYAIRAIEHVLTSRGTNFRDVFADFGAANAIARQWYSEGASYTTPKAPEVTPSTT
                     GVGGDLPMYHLSNDYVTLLPSSVTGEGATLTITLNLPDRVRGPEAYAVFFQKDGSIVP
                     LRFALNDAGDGALTGIPFAASQVNKAILVLTNASTRYRCWQRTTLSCRGTALDDVDFH
                     FGAQIG"
     gene            complement(846..1208)
                     /gene="folB"
                     /locus_tag="VG709_05605"
     CDS             complement(846..1208)
                     /gene="folB"
                     /locus_tag="VG709_05605"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWC32587.1"
                     /translation="MTGVVVEVHGLELHGHHGATERERREGQRFLFDVELVVADAAAA
                     SDDLADTVDYREVVSCVREVSDGHAFALLEALAAAVADALVARFPVTRVRVRVRKPEV
                     RLDVPVEYTAATVERGAV"
     gene            complement(1205..>1521)
                     /locus_tag="VG709_05610"
     CDS             complement(1205..>1521)
                     /locus_tag="VG709_05610"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWC32588.1"
                     /translation="VSEERICLDPGIGFGKTVEHNLELLARLGELAALGRPILVGASR
                     KRFLGRLLGDPDALTGPVAASVAAAVIAYERGASLFRVHDVREHVEALAAAHALARFA
                     AA"
BASE COUNT          241 a          551 c          525 g          204 t
ORIGIN      
        1 gtccagttcg cgtacgactg gatggaggat ccgtggctga tggaggcgac cgcgacctgg
       61 atcgaggacg atgtcttcga cgggatcaac gacaacctcc agttcctccc ggcgagcccg
      121 ctcagcttcg gcgctccgtg gcacgcgatc gaccacttcc acccgcacgg ctcgaaccag
      181 tacggcgcct ggatcttctt ccgcttcctc tcagagtact tcggatcgcc gacgcacccg
      241 agcagcgcgt ccgaccggac cgtcgtgcga gacatctggg cggccgccga cgggcgcggg
      301 aaggcgaacg gggggcggta cgcgatccgc gcgatcgagc acgtcctcac gagccgaggc
      361 acgaacttcc gcgacgtgtt cgccgacttc ggcgccgcca acgcgatcgc caggcagtgg
      421 tactccgagg gcgcgagcta cacgacgccg aaggcgcccg aggtgacgcc ttcgacgacg
      481 ggcgtcggcg gcgaccttcc gatgtaccac ctctcgaacg actacgtcac cttgctgccg
      541 tcgtcggtga cgggcgaggg cgccacgctc acgatcaccc tgaacctccc cgaccgggtt
      601 cgcgggcccg aggcgtacgc cgtcttcttc cagaaggacg gatccatcgt gccgctccgc
      661 ttcgcgctga acgatgccgg cgacggcgcc ctcaccggca ttccgtttgc ggcgagccag
      721 gtgaacaagg cgatcctcgt gctgacgaac gcgagcacgc gctaccgctg ctggcagcga
      781 acgacgttgt cctgccgcgg caccgcgctc gacgacgtcg acttccactt cggcgcccag
      841 atcggctaga cggcccctcg ctcgaccgtc gcggccgtgt actccacggg aacgtcgagg
      901 cgaacctccg gcttcctgac gcggacgcgg acacgcgtca cggggaaacg cgcgacgagc
      961 gcgtcggcca ccgcggcggc gagcgcctcg agcagcgcga atgcgtgccc gtcggagacc
     1021 tcgcggacgc acgacacgac ctcccggtag tcgaccgtat ccgcgaggtc gtcgctcgcc
     1081 gcggcggcgt ccgcgacgac gagctcgacg tcgaagagga agcgctgccc ctcgcggcgc
     1141 tcgcgttccg tggcgccgtg gtggccgtgc agctcgagcc cgtgaacctc gacgacgacg
     1201 cccgtcacgc ggcggcgaaa cgcgcgagcg cgtgggcggc ggcgagcgcc tcgacgtgct
     1261 cgcggacgtc gtggacgcgg aagagcgagg cgccccgctc gtaggcgatg acggccgccg
     1321 cgacgctcgc agccaccggc ccggtgagcg cgtcgggatc gccgagcagg cggccgagga
     1381 agcgcttccg cgacgcgccg accaggatcg gacgcccaag ggcggcgagc tcgccgagac
     1441 gcgcgaggag ctcgaggttg tgctcgaccg tcttgccgaa gccgatcccg gggtcgaggc
     1501 agatccgctc ttccgaaacc c
//
LOCUS       DASZOB010000350         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_199651, whole genome shotgun sequence.
ACCESSION   DASZOB010000350 DASZOB010000000
VERSION     DASZOB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199651"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VG709_05615"
     CDS             <1..901
                     /locus_tag="VG709_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWC32589.1"
                     /translation="LTVVLVEALKNTGLVREDAAIGLVFPALFSAGVILIARKADSVH
                     LDTDVVLLGEIAFAPFDRVVVAGWDLGPKSLVLMSSVLVLKVVLIALFYKELKLATFD
                     PALAVALGFAPGLIHYGLMTLVSVTAVGAFNAVGTILVVALVIAPPATAYLLTDRLAA
                     MLAASVGTGVASAIAGYWLAHALDASLAGSMATAAGALFALAFLFAPARGLVAGARRR
                     ARQRWEFPQTMLAIHLLNHEGKPEAAEESRVEHLAEHLRWDASFAAEVVARADRRGIL
                     VRDDGTLVLTERGRALARSALAR"
     gene            complement(902..1576)
                     /locus_tag="VG709_05620"
     CDS             complement(902..1576)
                     /locus_tag="VG709_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32590.1"
                     /translation="MSTLRAVEWEAHVERGRGRYEDGARRLPSNDPEARQKQLVRMAM
                     AASAVGLALSMQGRPEATEWLSRSADRYRESFAGAPAGSWGRLIGALKARLLAGDRDG
                     ARADAAWALEQGPATSESPIGRYAAALAALVLGDDETAGALASSLRAEPAEAFPRPVA
                     DALAALAARDGEAYAKAAKDTLTSFETRDDYLEDLPVADTVLALELLAEARGIRAGLR
                     SSLLPA"
BASE COUNT          241 a          679 c          649 g          253 t
ORIGIN      
        1 cctcaccgtc gtgctcgtgg aggcgctgaa gaacaccggc ctcgtacggg aggacgccgc
       61 gatcggcctc gtcttcccgg cactcttcag cgccggcgtg atcctgatcg cccggaaggc
      121 cgactcggtt cacctcgaca cggacgtcgt cctgctgggg gagatcgcgt tcgccccgtt
      181 cgaccgcgtc gtcgtggccg gctgggacct cggccccaag tcgctcgtgc tgatgagctc
      241 cgtcctcgtc ctgaaagtcg tgctgatcgc gctcttctac aaggagctca agctcgcgac
      301 gttcgacccg gcgctcgccg tcgcgctcgg attcgccccg ggcctcatcc actacggcct
      361 catgacgctc gtttccgtga cggcggtggg cgcgttcaac gccgtcggga cgattctcgt
      421 cgtcgcgctc gtgatcgcgc cgcccgcgac ggcgtacctc ctgaccgacc ggctcgcggc
      481 catgctcgcc gcgagcgtcg ggaccggcgt cgcgagcgcg atcgcgggct actggctcgc
      541 gcacgcgctc gacgcatcgc tcgcgggctc gatggcgacg gcggccgggg cgctcttcgc
      601 gctcgcgttc ctgttcgcgc ccgcgcgcgg gctcgtcgcc ggtgcgagac ggcgagcgcg
      661 ccaacgatgg gagtttccgc agaccatgct cgcgatccac ctcctgaacc acgagggcaa
      721 gccggaggcg gccgaggaga gccgcgtcga gcacctcgcc gagcacctgc gctgggacgc
      781 ctcgttcgcg gcggaggtgg tcgcgcgggc cgaccggcgc gggatcctcg tacgagacga
      841 cgggacgctc gtgctgacgg agcgcggacg cgcgctcgcc cggagcgctc tcgcgcgctg
      901 atcaggccgg caggagcgac gacctcaggc cggcccggat cccccgggcc tccgccagca
      961 actcgagcgc gagcaccgtg tccgcgactg gaaggtcctc gaggtagtcg tcacgcgtct
     1021 cgaacgacgt gagcgtgtcc ttcgcggctt tcgcgtaggc ctcgccgtca cgagccgcga
     1081 gcgcagccag cgcgtccgcg accggccgtg ggaacgcctc ggcgggctcg gcgcgaaggg
     1141 aggacgcgag ggcgccggcc gtttcgtcgt cgccgaggac gagcgcagcg agcgcggcgg
     1201 cgtagcggcc gatcggcgac tcgctcgtgg ccggcccctg ctcgagggcc cacgccgcgt
     1261 ccgcgcgtgc gccgtcacgg tcgccggcga ggagccgcgc cttcagggct ccgatcagcc
     1321 ggccccagct ccccgccggc gcaccggcga agctctcgcg gtaccggtcg gccgaacgcg
     1381 agagccactc cgtcgcctcg ggccggccct gcatcgagag cgcaaggccg accgcgctcg
     1441 cagccatcgc catccgcacg agctgcttct ggcgggcctc agggtcgttc gaggggagtc
     1501 ggcgcgcgcc atcctcgtac cgcccccggc cgcgctcgac gtgcgcctcc cactccacgg
     1561 cgcgcagcgt agacaccgga gctggaccgg atcaggacga gctttggagg gacctgctgc
     1621 cgcaggcagg cccccctcgc ttgtgtcgct agccggccat gcggaggcaa gccctcgaac
     1681 gagggttgca tgaccggagt aacgccgtca cggtgcgacg gggaaggggc gagcgacaaa
     1741 agtccctcgc cccttcccgc cgtaccgcag acgtgtcaaa ctacggaccc cagggagccc
     1801 ggagcggccg gggagcccga gg
//
LOCUS       DASZOB010000351         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128311, whole genome shotgun sequence.
ACCESSION   DASZOB010000351 DASZOB010000000
VERSION     DASZOB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128311"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1545
                     /locus_tag="VG709_05625"
     CDS             49..1545
                     /locus_tag="VG709_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWC32591.1"
                     /translation="MADVDPRPFPPGEYPVVVVGSGPGGLETSYFLRRLGVDHAVISK
                     DDAPGGMFRRFPLFDRLISWTKPYAPVAPDTREYEWYDWNSLLVTPPDTPVHVADFMD
                     GTSEFPSRREMEASLVAFAERHGVAVRYDCTWEATRRTGDGFVLATSDGEYRCRVAVF
                     AVGVTEPWKPPDIPGVEHVPHYMEMKPAEAYAGKSLYILGKATSAFEVADGLLPWASR
                     MVLSSPHEVRLSVIERSLAGLRARYTQPMEDHAIGGRSVLLLDAATERIDRTATGYRV
                     FLMGTTQPWNLELDVDEAVIATGVSTPLGDLPEIGVATYSRGGRLPAQTPFWESASVP
                     GIYFTGSITQGAIGLRRATGAGAVHGFRYAARMIAEHIAERFGVRTQRPLLRPDEVVP
                     LLLPEATSGPELWNQRSYLARAVAFDPDAGVLDEGVVPLAHFVDARGPDAVAVAVVAD
                     DERRPRAVAYVRRGTAVEEHLLEPSLLLDFRSGEHEAQLRSALKGLLP"
BASE COUNT          241 a          651 c          606 g          248 t
ORIGIN      
        1 gttccggatg ctccaggtcg aggccgcgcg gctgcggaac gcgatccggt ggcggacgta
       61 gacccgcgcc cgttccctcc cggcgagtac ccggtcgtcg tcgtcggaag cgggccgggc
      121 gggctcgaga cgagctactt cctccggcgc ctcggcgtcg accacgccgt gatctcgaag
      181 gacgacgcgc ccggcggcat gttccggcgc ttcccgctct tcgaccggct catcagctgg
      241 acgaagccgt acgcgcccgt cgcgccggac acgcgcgagt acgagtggta cgactggaac
      301 agcctcctgg tcacgccgcc ggacacgccg gtccatgtcg ccgacttcat ggacggcacc
      361 tcggagttcc catcgcgccg cgagatggag gcatcgctcg tcgcgttcgc cgagcgccac
      421 ggcgtcgcgg tcaggtacga ctgcacgtgg gaggcgacca gacgaaccgg cgacgggttc
      481 gtgctcgcga cgtcggacgg cgaataccgg tgccgcgttg cggtgttcgc ggtcggcgtc
      541 accgaaccgt ggaagccgcc cgacatcccg ggcgtggagc acgtccccca ttacatggag
      601 atgaagccgg cggaggcgta cgcgggcaag agcctctaca tcctcgggaa ggcgacgtcc
      661 gcgttcgagg tcgccgacgg gttgctcccg tgggcgagcc gcatggtgct ctcctccccg
      721 catgaggtcc gcctctccgt gatcgagcgc tcgctcgccg gcctgcgcgc ccgctacacg
      781 cagccgatgg aggaccacgc gatcggcggc cgcagcgttc tcctgctcga cgccgcgacc
      841 gaacggatcg accgcacggc aacgggctac cgcgtcttcc tcatggggac gacccaaccg
      901 tggaacctcg agctcgacgt cgacgaggcc gtgatcgcga ccggggtgtc gaccccgctc
      961 ggcgaccttc cggagatcgg cgtcgcgacg tactcgcgcg gcggccgcct ccccgcgcag
     1021 acgccgttct gggagagcgc ctcggtcccc ggcatctact tcacggggtc gatcacgcag
     1081 ggcgcgatcg gcctgcggcg cgcgacgggc gccggcgccg tgcacggttt ccgctacgcg
     1141 gcgcggatga tcgccgagca catcgccgag cggttcggcg tgaggacgca acggccgctc
     1201 ctccgcccag acgaggtcgt gccgctcctc ctccccgagg cgacgtcggg tccggagctc
     1261 tggaaccagc gctcgtacct cgcccgcgcg gtcgccttcg accccgacgc cggcgtcctc
     1321 gacgagggcg tggtgccgct ggcgcacttc gtcgacgctc gaggacccga tgccgtcgcg
     1381 gtcgcggtcg ttgccgacga cgaacggagg ccccgtgccg tcgcgtacgt gcgccgcggg
     1441 acggccgtcg aggagcacct cctcgaacca agcctcctcc tcgatttccg gagcggcgag
     1501 cacgaagccc agctccgctc ggctctgaag ggccttctcc cctagcgcga accgcgccag
     1561 ggccgcagtc cgaagaagcg gcccggacct gccgggtcgg aaccgatcga tcccggcgcc
     1621 tgtatggaag aggaaccatg atgcggaccc gggccgtcgc cgcactcctc gcggtcgccg
     1681 tggccggctg cgccgtcgtc ggccggcgcg acgccggccc cgcctcatcg ctcacaggcg
     1741 tagtcc
//
LOCUS       DASZOB010000352         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128315, whole genome shotgun sequence.
ACCESSION   DASZOB010000352 DASZOB010000000
VERSION     DASZOB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128315"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VG709_05630"
     CDS             <1..445
                     /locus_tag="VG709_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32592.1"
                     /translation="GRKRGMERQRQRHPDTGTPEDEGIPEMEGPPEGQEETSDSPAGM
                     VIPRDEPQASDEHGTTATEQRDGESLGERLAEEEPERAPGTARDQAGRLQEDDVAGVD
                     TEKDLVAEEADDDTLGLTAEEDAMRVQDAAPGAVDEPDDYVDERD"
     gene            496..1776
                     /gene="eno"
                     /locus_tag="VG709_05635"
     CDS             496..1776
                     /gene="eno"
                     /locus_tag="VG709_05635"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWC32593.1"
                     /translation="MAEIESIVAREILDSRGNPTVEVEVLLHDGAAGRAAVPSGASTG
                     EHEALELRDGDGKRFGGKGVLVAVRGVEETIAPALEGLDALEQREIDRVLLDLDGTLD
                     KSKLGANALLGVSLAVAHAAAASIELPLWRYLGGPNAHLLPVPLFNVINGGAHADNDL
                     ELQEFMLAPHGAASFSEALRWGAECLMSLRSLLKRRGMSTAVGDEGGFAPDVSTGAAA
                     CDLMVEAVRDAGLEPGDEVAFALDPAASELFADGRYRVEGRERSSEEMLRYFEELVGA
                     FPIVSIEDPLAEDDWDAWRTFTDALGDRVQIVGDDLFVTNPDRLVRGIAERSANAILV
                     KVNQIGTLTETLDVMSQAEESGFGRVVSHRSGETEDATIADLAVATGAGQIKSGGPQR
                     GARTAKYNRRLRIEEELGEGARYAGGAPYRRFRR"
     gene            1773..2099
                     /locus_tag="VG709_05640"
     CDS             1773..2099
                     /locus_tag="VG709_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWC32594.1"
                     /translation="MTARAVHARPRARLTPRGAILAVLVVALAMALAVPLRQYFEQRA
                     EIAGLERRLVELRAERDALEAKIRRLNDPAHLERVARRCLGMVRPGEIPFVTVPESGR
                     PRPSRC"
     gene            2184..2531
                     /locus_tag="VG709_05645"
     CDS             2184..2531
                     /locus_tag="VG709_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWC32595.1"
                     /translation="MGAVVEGTVQRITPYGAFLQLEDGRVGLVHISEIDRNYVKDVRE
                     HLRENDKVQAKVVAIKEDGKIDLSIKALQDPPPRAMRRGKDPELEQKLKRFMRQSEER
                     QVDFRRSIEGKRR"
     gene            2546..3940
                     /locus_tag="VG709_05650"
     CDS             2546..3940
                     /locus_tag="VG709_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWC32596.1"
                     /translation="MRGSALRLVARQLGREPQAAFEVVARCHDGHPLVVRNHPVDRSG
                     NPFPTLYWLTCPAATKAVSRLEAEGWIGRLADRAEADPNFGAALARAHDEHARDRGRF
                     VPDASGWGGVGGARAGVKCLHAHYANHLAGGRDPVGAWVAERVEPVHAEAPTRRRVAA
                     VDIGTNSIRLLVADERGGAVDEIARDLLITRLGQGVDDVGRIDPEALRRTMSVLERYV
                     VRARALGASEIRVAATSAVRDATDRAVLEREVERLAGVGPEVLSGTREAELSFLGATT
                     GLDAEPPYLVFDIGGGSTEIVVGSAEPEATASVDIGSVRLTERVRPSDPPSESDVARM
                     EDIAAALLAPAEHAVPAGRAATLVGVAGTTTTVQAIALGLDRYDPEAINGSILRLEDA
                     RRVARELARMTVADRRAVPVMTPGREDVIVAGSVILVEIFQRWRFDACIVSERDLLDG
                     LAIDAVRAATPPVP"
BASE COUNT          602 a         1394 c         1529 g          520 t
ORIGIN      
        1 gggtaggaag cgcggcatgg agcgacaacg acagcgccac cccgacaccg gcacgccgga
       61 ggacgagggg atccccgaga tggagggtcc tccggaagga caggaggaga cctcggactc
      121 gccggccggc atggtgatcc cgcgcgatga gccgcaggcg tcggacgagc acggaacgac
      181 cgccaccgag cagcgagacg gcgagtcgct cggcgagcgc ctcgcggagg aggagcccga
      241 gcgcgcgccg ggaacggcgc gcgaccaggc cgggcgcctt caagaggacg acgtcgcggg
      301 cgtcgacacg gagaaggacc tcgtcgccga agaggcggac gacgacacgc tcgggctcac
      361 ggcggaggag gacgcgatgc gcgttcagga cgccgccccg ggggcggtcg acgagccgga
      421 cgactacgtc gacgagcgcg actagcgacc gaaatgcccg gccgggcccc ggccgccacc
      481 tagaatcgag cggccgtggc ggagatcgag agcatcgtcg cgcgcgagat cctcgattcg
      541 cgcggaaacc cgaccgtgga ggtcgaggtc ctgctgcacg acggcgcggc cgggcgtgcg
      601 gcggtcccgt ccggggcgtc gaccggcgag cacgaggcgc tcgagctgcg ggacggcgac
      661 ggcaagcgct tcggcggaaa gggcgtgctc gtggcggttc gcggcgtcga ggagacgatc
      721 gcgccggcgc tcgagggact cgacgcgctc gagcagcgag agatcgaccg cgtcctcctc
      781 gacctcgacg gaaccctcga caagtcgaag ctcggcgcga acgcgttgct cggcgtctcc
      841 ctcgccgtcg cacacgcggc tgccgcgtcg atcgagctgc cgctgtggcg gtatctgggc
      901 gggccgaacg ctcatctgct gccggtcccg ctgttcaacg tcatcaacgg cggcgcgcac
      961 gcggacaacg acctcgagct ccaggagttc atgctggctc cgcacggcgc agcgtcgttc
     1021 tccgaggcgc tccggtgggg tgcggagtgc ctcatgtcgc ttcgctcgct gctcaagcga
     1081 cgtggcatgt cgacggccgt cggagatgag ggaggcttcg cccccgacgt ttcgacgggc
     1141 gcggcggcgt gcgacctcat ggtggaggcc gtacgcgacg cggggctcga gcccggcgac
     1201 gaggtcgcgt tcgcgctcga cccggccgct agcgagctgt tcgcggacgg gcgataccgc
     1261 gtcgaggggc gcgagcggtc gtcggaggag atgctccgct acttcgagga gctcgtcggc
     1321 gcgttcccga tcgtctcgat cgaggacccg ctggccgagg acgactggga cgcgtggcgc
     1381 acgttcacgg acgcgctggg cgaccgcgtg cagatcgtcg gggacgacct gttcgtgacg
     1441 aacccggacc gcctcgtgcg cgggatcgcc gagcgctccg ccaacgcgat tctcgtgaag
     1501 gtgaaccaga tcgggacgct caccgaaacg ctcgacgtga tgtcgcaggc cgaggagagc
     1561 gggttcggcc gcgtcgtctc ccaccgctct ggcgagaccg aggacgcgac gatcgccgac
     1621 ctcgcggtgg cgaccggcgc gggacagatc aagtcgggcg gtccccagcg cggcgcgcgg
     1681 acggcgaagt acaaccggcg gcttcggatc gaggaggagc tcggcgaggg agcgcgctac
     1741 gccgggggcg cgccgtaccg gaggttccgc cggtgaccgc tcgcgccgtc cacgcgaggc
     1801 cgcgggcgcg cctgacgccg cgcggcgcga tcctcgccgt gctcgtcgtc gccctcgcca
     1861 tggcgctggc ggtcccgctc cgccagtact tcgagcagcg cgcggagatc gccgggctcg
     1921 agcggcgcct cgtcgagctc cgcgccgagc gcgacgcgct cgaggcgaag atccgccgcc
     1981 tcaacgaccc cgcccacctc gagcgggtcg cccgcaggtg cctcggcatg gtccgtccgg
     2041 gggagatccc gttcgtcacg gttcccgaga gcggccggcc ccggccctcc cgctgttagc
     2101 ggattgacgg tttctccgag cgcgtgggat agtcgatcgt cacccgccca gctagggaag
     2161 gagtcgtcat cgcggagatc gagatcggcg cggtcgtgga ggggaccgtg cagcgcatca
     2221 ccccgtacgg ggcattcctc cagctcgagg acggacgcgt cgggctcgtc cacatctcgg
     2281 agatcgaccg caactacgtc aaggacgtcc gcgagcacct ccgcgagaac gacaaggtcc
     2341 aggccaaggt cgtcgcgatc aaggaagacg gcaagatcga cctgtcgatc aaggccctgc
     2401 aggatccgcc gccccgcgcg atgaggcgcg gcaaggaccc ggagctcgag cagaagctca
     2461 agcgcttcat gcgtcagagc gaggagcgcc aggtcgattt ccggcgctcg atcgaaggca
     2521 agcggcggta gccgccggct tcgagctgcg cggctccgcc ctccgcctcg tcgctcgtca
     2581 gcttggacgt gagccacagg ctgcgttcga ggtcgttgct cggtgccacg acgggcatcc
     2641 gctcgtggta cggaatcatc cggtcgaccg cagcggaaac ccgttcccca cgctctactg
     2701 gctgacgtgt ccggcggcga cgaaggccgt ctcgcggctc gaggcggagg gatggatcgg
     2761 caggctcgcc gatcgcgcgg aggccgaccc gaacttcggt gcggcgctgg cgcgcgcaca
     2821 cgacgagcac gcacgggacc gtggacggtt cgtcccggac gcctcgggct ggggcggcgt
     2881 tgggggcgcg cgcgcgggcg tcaaatgcct ccatgcgcac tacgctaacc acctcgcggg
     2941 gggccgcgat ccggtcggcg cgtgggtcgc tgagcgggtc gagcccgtcc acgcggaagc
     3001 gccgacgcgt cgccgcgtcg ccgcggtgga catcgggacg aactccatcc gtctgctcgt
     3061 cgccgacgag cggggcggcg ccgtcgacga gattgcgcgg gatctcctga tcacgcggct
     3121 gggccagggc gtcgacgatg tcggtcggat cgacccggag gcgctgcgcc ggacgatgtc
     3181 ggtcctcgag cggtacgtcg tgagggcgag ggctcttggc gcgtccgaga tccgggtcgc
     3241 cgccacgagc gccgtccgcg acgcgacgga ccgagctgta ctcgagcgcg aggtcgagcg
     3301 gctcgccggc gtcggccccg aggtcctctc cggaacgcgc gaggccgagc tctcgttcct
     3361 cggcgcgacg accgggctcg acgccgagcc gccctacctc gtgttcgaca tcggaggcgg
     3421 cagcacggag atcgtcgtag ggtcggcgga gccggaggcg acggcctccg tcgacatcgg
     3481 tagcgtgcgc ctcaccgagc gcgtgcgacc ttccgaccct ccgagcgagt ccgatgtcgc
     3541 tcggatggag gacatcgccg ctgcgctgct cgcgcccgcg gagcacgccg tgcccgccgg
     3601 ccgagccgcg acgctcgtcg gcgtcgcggg gacgacgacg accgtgcagg cgatcgcgct
     3661 cgggctcgac cgctacgacc ccgaggcgat caacgggtcg atcctgcgcc tcgaggacgc
     3721 gcggcgcgtc gcgcgcgagc tcgcccgcat gacggtcgcc gaccggcgcg cggtgccggt
     3781 catgacgccg gggcgcgagg acgtcatcgt cgcggggtcg gtgatcctcg tcgagatctt
     3841 ccagagatgg cggttcgacg cgtgcatcgt gtccgagcgc gaccttctcg acggcctcgc
     3901 gatcgacgcc gtgcgtgcgg cgacgcctcc ggtaccctga ccgccgtggc gaagagaacg
     3961 cgacagcggc agctgaagaa gctcgccgag cggcgggcgc gcgaccggcg gcggcgcgac
     4021 aggcggaagg cgatcctcgc cggcg
//
LOCUS       DASZOB010000353         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_13581, whole genome shotgun sequence.
ACCESSION   DASZOB010000353 DASZOB010000000
VERSION     DASZOB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13581"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VG709_05655"
     CDS             complement(<1..234)
                     /locus_tag="VG709_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWC32597.1"
                     /translation="MATSTRDYLSIGEVLITLKPEFPDITISKIRFLESEGLIDPERT
                     PSGYRKFYESDVDRLRRILKLQRDEYLPLRVIKD"
     gene            complement(234..695)
                     /locus_tag="VG709_05660"
     CDS             complement(234..695)
                     /locus_tag="VG709_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWC32598.1"
                     /translation="MYCTRCGHRNPQDARFCAKCGHPLQDETTLNLAQVELEDETGED
                     IHLPLDQLNERQALLVVKRGPNAGSQLLIEKDVTTTGRHPESDIFLDDITVSRRHAEF
                     RRKDERFQIHDMGSLNGTYVNRNRVETTELASGDEIQIGKFKLMFFAGKGE"
     gene            complement(808..1203)
                     /gene="gcvH"
                     /locus_tag="VG709_05665"
     CDS             complement(808..1203)
                     /gene="gcvH"
                     /locus_tag="VG709_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWC32599.1"
                     /translation="MEYPSDLRYTSEHEWAAPEEGGRVRVGVTDFAQDALGDVVYVDL
                     PAVGADLRAGETFGEVESTKSVSDLFSPVSGRVVERNEQLEDAPELVNQDPYGEGWMI
                     VIEMEDASDLDELMDAEAYRRTVEEGGEG"
     gene            complement(1258..>2039)
                     /locus_tag="VG709_05670"
     CDS             complement(1258..>2039)
                     /locus_tag="VG709_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HWC32600.1"
                     /translation="RVGAEVLAVNAELDEDKAVMSREEAGRNLDQLAQLVRSSGADLG
                     VMLDATGERMRLVDRRGRKIPLGQALVAFVDLISRTHDAPAVAVPVATSRVVEDIVGR
                     AGGRVVWSGISSSALSYASREEGVVFAGAEGGGYIFPAFLPSYDALVALAKLLELLAR
                     TGSRLDEVIDRLPSTHVARIDVATPWEDKGAVMRRLVERLDGQETVTIDGVKAFRGRD
                     WALVVPHPQEPLVRVWAESDGPDTSRELAGEFAALVEDLRG"
BASE COUNT          311 a          728 c          642 g          358 t
ORIGIN      
        1 gtccttgatc acgcgcagcg gcagatattc gtcgcgctgg agcttgagga tccggcggag
       61 ccggtccacg tcgctctcat agaacttccg ataccccgac ggggtccgct ctgggtcgat
      121 caggccctcg ctctcgagga accggatctt cgagatcgtg atgtcgggga actcgggctt
      181 gagcgtgatg agcacctctc cgatcgagag gtagtcccgc gtcgaggtcg ccatcactcg
      241 cccttcccgg cgaagaacat cagcttgaac ttcccgatct ggatctcgtc gccgctcgcg
      301 agctccgtgg tctcgacgcg gttccggttg acgtaggtcc cgttgaggct ccccatgtcg
      361 tggatctgga accgctcgtc cttccggcgg aactcggcgt gacgccgcga caccgtgatg
      421 tcgtcgagga agatgtcgct ctcgggatgg cgcccggtcg tcgtcacgtc cttctcgatg
      481 agcaactgcg agcccgcgtt cggcccgcgc ttgacgacga gaagagcttg cctctcgttc
      541 agctgatcca gagggagatg gatgtcctct ccggtctcgt cctcgagctc gacctgcgcg
      601 aggttgagcg tcgtctcgtc ctgcagcgga tggccgcact tcgcgcagaa gcgcgcgtct
      661 tgtgggttcc ggtgaccgca tcgggtgcag tacacgtctc cctcccgcgg agtctcagga
      721 agcaacgatt gactgtatac gacacgtcga actcggtcaa tcgttccgtc agcgagaggt
      781 cgcagaacct cgacctgaac cggagggcta tccctcgccg ccctcctcga ccgtgcgccg
      841 gtacgcttcg gcatccatga gctcgtcgag gtcgctcgcg tcctccatct ctatgacgat
      901 catccagcct tcgccgtacg gatcctggtt cacaagctcc ggcgcgtcct cgagctgctc
      961 gttacgctcc acgacgcgac ccgagaccgg actgaagagg tccgacacgg atttggtcga
     1021 ctcgacctcg ccgaacgtct ccccggcgcg caagtcggca ccgacggccg gcaggtccac
     1081 gtacacgacg tcgccgagcg cgtcctgcgc gaaatccgtg acgccgacgc gcacgcgccc
     1141 cccctcctct ggggccgccc attcgtgctc gctcgtgtac cggagatcgg acggatattc
     1201 catggtctcc ccctcggtcg gtactcgggc gtccgtgact gtacccgcgt tcggcggtca
     1261 gccgcgcaga tcttcgacga gcgccgcgaa ctctccggcc agctctcggg acgtgtcggg
     1321 tccgtcggac tccgcccaca cgcgcaccag cggctcctgc ggatgaggaa cgaccaacgc
     1381 ccagtccctt ccccggaacg ccttcacgcc gtcgatagtc acggtctcct gcccgtcgag
     1441 gcgctcgacg aggcgtcgca tcaccgcgcc cttgtcctcc cagggcgtcg cgacgtcgat
     1501 ccgggcgacg tgcgtggacg gcagccggtc gatgacctcg tcgagcctcg aaccggtccg
     1561 agcgaggagc tcgaggagtt tcgccagcgc gaccagcgca tcgtaggacg gaaggaacgc
     1621 ggggaagatg tatccgccgc cctccgctcc cgcgaacacg acgccctcct cccggctcgc
     1681 gtacgagagc gccgagctcg agatgccgct ccacacgacg cgtccgcccg ctcgcccgac
     1741 gatgtcctcg accacgcggg aagtcgcgac ggggacggcg accgccggcg cgtcatgggt
     1801 ccgcgagatg aggtcgacga aggcgacgag cgcctgtcct agcggtatct tccgcccgcg
     1861 ccggtcgacg aggcgcatgc gctcgccggt ggcgtcgagc atcacgccga ggtccgcgcc
     1921 gctcgagcgg acgagctgcg cgagctgatc gaggttccgc cctgcctcct cgcgcgacat
     1981 gacggccttg tcctcatcga gctcggcgtt gaccgcgagc acctcggcgc cgacgcgcc
//
LOCUS       DASZOB010000354         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_68516, whole genome shotgun sequence.
ACCESSION   DASZOB010000354 DASZOB010000000
VERSION     DASZOB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68516"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1831
                     /locus_tag="VG709_05675"
     CDS             194..1831
                     /locus_tag="VG709_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWC32601.1"
                     /translation="MDKPRHGGHLLARALRHEGVQHLFTLSGGHIAPIYDGCVDEGIR
                     VVDFRHEQAAAHAADGWARVTGRPGVAAVTAGPGVTDSVTGIANAFYADSPMLVLGGK
                     SPLSELEMGSLQEMDHIALVRSITKWAKTCWETRRIADYVTEALRHATSGRKGPAFLD
                     IPLEIQFNAWTPDVQVPEGYRTDAKPQGDPQFMRRAAAMLRDAEHPVVFAGSGVRWGD
                     GSDALAALADALKVPVFLNSLGRGCLPADHAYFFSAARKYALGKADIVLALGVDWDFR
                     LGFGRRGISEAARVIQVDVDGRHIGRNRPVDVGIVGDPGKVIEQLLAEGLGSTEEPAW
                     TSEVRDEENRLRDEARAGMESEAVPIHPQRFAREIRDFLDRDAIVVGDGGDIVGISAS
                     ILPVYRPGHWLDPGPFGCLGVGPSFAMAAKLAKPDKQVLIVYGDGSFGLNAMEYESAI
                     RQGIPFVGIIGNDGAWGQIKVAQEALYGPGNAPAAFLSQQTGYEKMVEGLGGYGERVT
                     EPGQIRPALERAFAAGVPACINVMVDASLMKRASYLG"
BASE COUNT          289 a          658 c          721 g          284 t
ORIGIN      
        1 gtgctcctgc tcgaagtacg tcctgaagtc ggggagcggg ccgcgggcgt cggcgatcgg
       61 ggacgccacc gccgtcctcg ctccctcgtc catcggagcc atgaacgctc agcatggcgc
      121 gttccgttta catggccgga agattgggcc gagttccggc ccggaacgcc ggtcgcgtac
      181 gattcgctct cgcatggaca agcctcggca cggcggtcat ctgctcgctc gggcgctccg
      241 gcacgagggc gtccagcacc tcttcacgct gtccggcggc cacatcgcgc cgatctacga
      301 cggctgcgtc gacgagggta tccgcgtcgt cgacttccgc cacgaacagg cggcagcgca
      361 cgcggccgac gggtgggcgc gcgtcacggg ccgtccgggc gtcgcggcgg tgaccgcggg
      421 ccccggggtc accgactccg tgaccggcat cgcgaatgcg ttctacgccg actcgccgat
      481 gctggtgctc ggcggcaaga gcccgctgtc ggagctcgag atggggtcgc tgcaagagat
      541 ggaccacatc gcgctcgtgc ggtcgatcac gaagtgggcg aagacgtgct gggagacgcg
      601 gcggatcgcc gattacgtga ccgaggcgct gcgccacgcg acgtccgggc ggaaggggcc
      661 ggcgttcctc gacatccccc tcgagatcca gttcaacgcg tggacgcccg acgtccaggt
      721 gccggagggt taccgcaccg acgccaagcc gcagggcgac ccgcagttca tgaggcgcgc
      781 cgcggcgatg ctccgcgacg ccgagcaccc cgtcgtcttc gcgggatcgg gcgtcaggtg
      841 gggtgacggc tcggacgcac tcgcggcgct ggccgacgcg ctgaaggtcc cggtcttcct
      901 gaactcgctc ggacgtggat gcctccctgc cgaccacgcg tacttcttct cggcggcgag
      961 gaagtacgcg ctggggaagg ccgacatcgt gctggcgctc ggcgtcgact gggacttccg
     1021 tctcggcttc ggccgccgcg ggatcagcga ggccgctcgc gtgatccagg tcgacgtcga
     1081 cgggcgtcac atcggacgga accgtccggt cgacgtcggg atcgtcggcg accccggcaa
     1141 ggtgatcgag cagctcctcg cggagggact cgggagcacc gaagagccgg cgtggacgtc
     1201 cgaggtacga gacgaggaga accggctccg cgacgaggcg cgcgccggga tggagtccga
     1261 ggcggtcccg atccacccgc agcggttcgc gcgcgagatc cgggacttcc tcgaccgcga
     1321 cgcgatcgtg gtcggcgatg gaggcgacat cgtagggatc agcgcgtcga tcctgccggt
     1381 gtaccggccg ggtcactggc tcgatcccgg cccgttcggg tgcctcggcg tggggccgtc
     1441 gttcgcgatg gcggccaagc tcgcgaagcc cgacaagcag gtgctgatcg tctacggcga
     1501 cgggtcgttc gggctcaacg cgatggagta cgagagcgcg atccgccagg ggatcccgtt
     1561 cgtcgggatc atcggcaacg acggcgcctg gggacagatc aaggtggcgc aggaggcgct
     1621 gtacgggccc gggaacgccc cggccgcgtt cctctcgcag cagacgggct acgagaagat
     1681 ggtcgagggg ctcggcgggt acggggagcg cgtgaccgaa ccgggccaga tccggccggc
     1741 gctggagcgg gcgttcgcag cgggcgtccc ggcatgcatc aacgtgatgg tcgacgcctc
     1801 gctgatgaag cgcgcgtcgt acctcggcta gacgcgccgc ttcgcctcgg ccgcgaaggc
     1861 cgctcgctcc actagcaccc gcaggtgcgt tccgcggccg acgacgccgc cgggatcaga
     1921 cgcctcgaag ccgaaccgga gcttccttcc gt
//
LOCUS       DASZOB010000355         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_148690, whole genome shotgun sequence.
ACCESSION   DASZOB010000355 DASZOB010000000
VERSION     DASZOB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148690"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VG709_05680"
     CDS             <1..649
                     /locus_tag="VG709_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HWC32602.1"
                     /translation="ADALGRILGEAPRLSVAGRTDAGVHAEGQVVSFETDAEPPPLQR
                     ALNAMLGPEVAVREARAMPQGFDARHSATAREYRYRMRTDEVPDPFSARYEWHRPGSY
                     DQSAMRRAARHLVGERDFASFCRPPKDGGSTVRRLERLTVRRVGERLEIGARANAFLH
                     QMVRSLVGTLVAVGDGRIDPDAMPDVLAARDRAAAGRVAPPHGLVLVRVLYGGPK"
     gene            675..1130
                     /gene="rplM"
                     /locus_tag="VG709_05685"
     CDS             675..1130
                     /gene="rplM"
                     /locus_tag="VG709_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWC32603.1"
                     /translation="MKTYSPKAKEIQRRWYVVDADGVVLGRLASEVASILRGKHKPTF
                     APHMDGGDHVIVVNASRVRLTGDKADKKIYRRHSGYPGGLREVSFRKMIAQRPQEVVE
                     RAVRGMLPSNRLGRQMARKLNVYAGPEHPHEAQRPAPLRVGEIPKVEES"
     gene            1131..1535
                     /gene="rpsI"
                     /locus_tag="VG709_05690"
     CDS             1131..1535
                     /gene="rpsI"
                     /locus_tag="VG709_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWC32604.1"
                     /translation="MATEATQERTRATGRRKEAVVRVRIAPGSGRFELNGRALEEYFP
                     SRSQRMLVTAPLRTAGKEKEIDVVASLEGGGTTGQAGALRHAIARALVDMDASLRGAL
                     KSEGFLTRDAREKERRKYGLKKARKAPQFSKR"
     gene            complement(1522..>1801)
                     /locus_tag="VG709_05695"
     CDS             complement(1522..>1801)
                     /locus_tag="VG709_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32605.1"
                     /translation="VILALVGGAPVVAARYLPKTGGVMAGVGLERFVVEMNGYRHEQG
                     VDLVKSAPTEQRTVPEKLARLRAQQDEELRTFLSGGRRPNDRSTATAC"
BASE COUNT          267 a          647 c          663 g          224 t
ORIGIN      
        1 cgccgacgcg ttgggacgca tcctcggcga ggcgccgcgc ctgtcggtcg cggggcggac
       61 cgatgccggc gtccacgccg aggggcaggt cgtgagcttc gagaccgacg ccgagccgcc
      121 gcccctacag cgcgcgctga acgcgatgct cggtcccgag gtggccgtcc gggaggcgcg
      181 cgcgatgccc caggggttcg acgcgcggca cagcgcgacc gcccgcgagt accggtaccg
      241 catgcgaacc gacgaggtgc ccgatccgtt ctcggcgcgc tacgagtggc acaggccggg
      301 gtcgtacgac cagagcgcga tgcggcgagc cgcgcgtcac ctcgtcggcg agcgcgactt
      361 cgcctcgttc tgccggcccc cgaaggacgg aggctcgacc gtccggcgcc tggagcgcct
      421 cacggtccgc cgcgtcgggg agcggctcga gatcggcgcg agggcgaacg cgttcctcca
      481 ccagatggtt cggagcctcg tcgggacgct cgtcgcggtc ggcgacgggc gcatcgatcc
      541 cgacgcgatg ccggacgtcc tggccgcacg cgaccgagcg gctgcgggcc gcgtcgcgcc
      601 gccgcacggg ctcgtgctcg tgcgggtcct ctacgggggg ccgaagtgag gtcgtgtacc
      661 ctatgtcggt tcggatgaag acctacagcc ccaaggccaa ggagatccaa cggcggtggt
      721 acgtcgtcga cgccgacggc gtcgtgctcg gacgcctggc gtccgaggtc gcgagcatcc
      781 tgcgcgggaa gcacaagccg acgttcgccc cgcacatgga cggcggcgac cacgtcatcg
      841 tcgtcaacgc gtcgcgggtg cgcctcaccg gcgacaaggc cgacaagaag atctaccggc
      901 gtcactccgg gtaccccggc gggctgcgcg aggtctcgtt ccgcaagatg atcgctcagc
      961 gccctcagga ggtcgtggag cgagccgtcc gcggcatgct cccgagcaac cgcctcggcc
     1021 gtcagatggc gcggaagctc aacgtctacg ccgggcccga gcacccgcac gaggcccagc
     1081 gcccggcgcc gctgcgcgtc ggcgagatcc cgaaggtgga ggagtcctag atggcgaccg
     1141 aggcgaccca ggagcgcacc cgcgcgaccg gcaggcggaa ggaggcggtc gtccgcgtgc
     1201 ggatcgcgcc ggggagcggt cgcttcgagc tcaacggccg tgcgctggag gagtacttcc
     1261 cgtcgcggag ccagcggatg ctggtcacgg cgccgctgcg caccgccggc aaggagaagg
     1321 agatcgacgt cgtcgccagc ctcgagggcg gagggacgac gggccaggcc ggcgcgctgc
     1381 gccacgcgat cgcgcgcgcc ctcgtcgaca tggacgcgtc gctccgcggg gccctcaaga
     1441 gcgaagggtt cctgacgcgc gacgcgcgcg agaaggaacg ccggaagtac gggctcaaga
     1501 aggcccgcaa ggcgccgcag ttcagcaagc ggtagcggtc gaccggtcgt tcgggcgcct
     1561 ccctcccgac aggaacgtcc tgagctcctc gtcctgctgc gcgcgcagcc gcgcgagctt
     1621 ctcggggacc gtcctctgct cggtcggcgc actcttcacg aggtcgacgc cttgctcgtg
     1681 ccggtagccg ttcatctcga cgacgaaccg ctcgaggccc acccccgcca tcacgccgcc
     1741 cgtcttcggg agataccgtg cggcgacgac gggcgccccg ccgacgagcg cgaggatcac
     1801 c
//
LOCUS       DASZOB010000356         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_88370, whole genome shotgun sequence.
ACCESSION   DASZOB010000356 DASZOB010000000
VERSION     DASZOB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88370"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1445
                     /locus_tag="VG709_05700"
     CDS             6..1445
                     /locus_tag="VG709_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWC32606.1"
                     /translation="MLDVVTSAVETASRAGADYADARWVSEETESLSVRNQEMEGIDR
                     SRSEGVGIRVLVGGYWGFAATARTTEDEIAAAGRLAVETARAASRLPRDPVRLSPVDP
                     VSDVWETTVQEDPFAVPLEEKVALLMEASRRMQGVRGLSFAEAMLDFYRKRQAFASSE
                     GARIEQTITHSGGGIEATAIGDGEMQRRSFPNSFRGHIAAAGYEHIRSLPLVEEAERV
                     ASEAVQLLSAPDCPSLETTLILDAQQMVLQVHESIGHPIELDRVLGMEAAYAGTSFLS
                     PEDRGRLRYGSEHVSVTADATLAGGLGTYGYDDEGVPAQRFPIIESGTFRDFVSSRET
                     AAVLGDRSNGTMRADGWENLPLIRMTNINLEPGEGTLAEIIGDTKDGIFMSTNTSWSI
                     DDRRLNFQFGCEAAWRVRDGKLTEMYRNPNYTGITPEFWGSCDAVGGQEEWTVWGTPN
                     CGKGQPGQTMRVGHGTAPARFRDVRVGVR"
     gene            1445..2797
                     /locus_tag="VG709_05705"
     CDS             1445..2797
                     /locus_tag="VG709_05705"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWC32607.1"
                     /translation="MPERIGPDEIRAVAEAALDVRGADGVEVLLIHEWTGLTRFADSA
                     IHQSTAREETGLRVRVVSRGRVGVSAANDLSKEGAAAAARSALELAEVAAPDPLFPGL
                     APRADVPARDDAYDEATATATPDARAEAVAELVGRCGDGFLAAGAFETSASEVALANT
                     EGQFAYAPMTQAQISTVVAGGDGGNGFAEKTAVRASELDAGAIGDRAYRKARDSQGAR
                     ELAPGRYEVVLEPAAVATLVGFLSWLGFGGRALIEGRSCFSDRLGEQLMSEQIKVYDD
                     ARSPLTIGLPFDFEGTPKRRVELIERGVPVGGVHDRRSAKQAGTESTGHALPPPNPEG
                     AFPLNVFLDAGNADMADMVNATSRGLLVTRFHYSNVVHPKDAIITGMTRDGTWLVEDG
                     EVRHPVKNLRFTQSIIEALRDVELVGLEAKLASEFFFAASRVPALKISSFQFTGTTDH
                     "
BASE COUNT          427 a          998 c         1051 g          385 t
ORIGIN      
        1 catccgtgct cgacgtcgtg acgtccgccg tggagaccgc cagcagggcc ggggccgact
       61 acgccgacgc ccgctgggtg agcgaggaga ccgagtcgct ctccgtccgg aaccaggaga
      121 tggaggggat cgaccggtcg cgctccgagg gcgtcggcat ccgcgtcctc gtcggcggct
      181 actgggggtt cgcggcgacg gctcgaacta ccgaggacga gatcgccgcc gccggacgcc
      241 tcgccgtcga gaccgcgcgg gccgcgtctc gcctcccccg cgaccccgtg cggctctccc
      301 ccgtcgatcc cgtgtccgac gtgtgggaga ccacggtcca ggaggatccg ttcgcggtcc
      361 cgctcgagga gaaggtcgcg ctgttgatgg aggcgtcgcg gcggatgcag ggcgtccggg
      421 ggctgtcgtt cgccgaggcg atgctcgact tctaccggaa gcggcaggcg ttcgcctcgt
      481 cggagggcgc gcggatcgag cagacgatca cgcactccgg cggagggatc gaggcgacgg
      541 cgatcggcga cggcgagatg cagcgacgct cgttcccgaa ctcgttccgc ggccacatcg
      601 cggctgccgg ctacgagcac atccggtcgc tcccgctcgt cgaggaagca gagcgcgtcg
      661 cgagcgaggc cgtccagctc ctatcggcgc ccgactgccc gtcgctcgag accacgctga
      721 tcctcgacgc gcagcagatg gtgctccagg ttcacgagtc gatcggtcat ccgatcgagc
      781 tcgaccgcgt cctcgggatg gaggccgcgt acgccgggac gtcgttcctc tcgccggagg
      841 accgaggacg cctgcgctac ggctccgagc acgtgagcgt cacggccgac gcgacgctgg
      901 ccggcggcct cgggacgtac ggctacgacg acgagggggt ccccgcgcaa cgcttcccga
      961 tcatcgagag cggcacgttc cgggacttcg tctcgagccg cgagaccgcc gccgtgctcg
     1021 gcgaccgctc gaacggcacg atgcgcgcgg acgggtggga gaacctgccg ctgatccgca
     1081 tgaccaacat caacctcgag cccggcgagg ggacgctcgc cgagatcatc ggcgacacga
     1141 aggacggcat cttcatgtcg acgaacacgt cgtggtcgat cgacgaccgc cggctcaact
     1201 tccagttcgg gtgcgaggcg gcctggcgcg tgagggacgg gaagctcacc gagatgtacc
     1261 ggaacccgaa ctacaccggg atcacgccgg agttctgggg atcgtgcgac gcggtcggcg
     1321 ggcaggagga atggacggtg tggggcacgc cgaactgtgg caagggccag cccggccaga
     1381 cgatgcgcgt cggccacggc accgcgcccg cgcgcttccg cgacgtccgc gtcggggtgc
     1441 gctgatgccc gagcgcatcg ggccggacga gatccgcgcc gtcgccgagg cggcgctcga
     1501 cgtgcgcggc gccgacgggg tcgaggtgct cctgatccac gagtggacgg ggctcacgcg
     1561 gttcgccgac tcggcgatcc accagagcac cgcgcgcgag gagacgggcc tgcgcgtccg
     1621 agtcgtgagc agaggacgtg tcggggtgtc cgcggcgaac gacctctcga aggagggagc
     1681 cgccgcggcg gcgcgcagtg cgctcgagct cgcggaggtc gcggcgcccg acccgctgtt
     1741 ccccgggctc gcgccgaggg ccgacgtacc ggcgcgcgac gacgcgtacg acgaggcgac
     1801 cgcgacggcg acgcccgacg cgcgcgccga ggcggtcgcc gagctcgtcg gccggtgcgg
     1861 cgacggcttc ctcgccgccg gcgcgttcga gacctcggcg tccgaggtcg cgctcgcgaa
     1921 caccgaggga cagttcgcgt acgcgccgat gactcaagcg cagatctcga cggtcgtcgc
     1981 tggcggcgac ggcgggaacg gcttcgccga gaagaccgcc gtccgcgcct ccgagctcga
     2041 cgccggcgcg atcggcgacc gcgcgtaccg gaaggctcgt gacagccagg gcgcgcgcga
     2101 gctggctcca gggcggtacg aggtcgtcct cgagccggcg gcggtcgcga cgctcgtcgg
     2161 gttcctgtcg tggctgggct tcggcggccg cgcgctcatc gaggggcggt cctgcttctc
     2221 ggaccggctc ggcgagcagc tgatgtccga gcagatcaag gtctacgacg acgcccgatc
     2281 cccgctcacg atcggtctcc cgttcgactt cgaggggacg ccgaagcggc gcgtcgagct
     2341 gatcgagcgc ggcgtgcccg tcggcggggt ccacgatcgc cgaagcgcga agcaggccgg
     2401 gacggagtcg acgggccacg cgctcccacc gccgaacccg gagggggcct tcccgctcaa
     2461 cgtcttcctc gacgcgggga acgccgacat ggcggacatg gtcaacgcga cgtcgcgagg
     2521 gctcctcgtg acgcgattcc actactcgaa cgtcgtacat cccaaggacg cgatcatcac
     2581 cgggatgacg cgcgacggaa cgtggctcgt cgaggacggc gaggtacggc acccggtgaa
     2641 gaacctgcgg ttcacgcagt cgatcatcga ggcgctgcgc gacgtggagc tcgtgggcct
     2701 cgaggccaag ctcgccagcg agttcttctt cgcggcctca cgcgtcccgg cgctcaaaat
     2761 ttcctcgttc cagttcaccg gcaccaccga ccactagcga cacaagggcg gcgggccgct
     2821 accctctcgc gcgtgctgct cgcgttcgcg ctgctcggcg t
//
LOCUS       DASZOB010000357         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128405, whole genome shotgun sequence.
ACCESSION   DASZOB010000357 DASZOB010000000
VERSION     DASZOB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128405"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="sucD"
                     /locus_tag="VG709_05710"
     CDS             <1..832
                     /gene="sucD"
                     /locus_tag="VG709_05710"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWC32608.1"
                     /translation="TGREGTFHAMNNRRYGTDVVAGVTPGKGGQDVEGVSVFDTVHEA
                     VREAGANTAMVFVPPRFAADSILEAADAGVSLVVAITEGIPAHDMLRVYNHIRPRGVT
                     LIGPNCPGVLSPGKANVGIIPAEIFGEGGVGLVSRSGTLTYQIGHELTQLGIGQSTIV
                     GIGGDPIVGSSFVDVLAKFQGDPETEVVVMVGEIGGAEEEKAAAVIDAEMSKPVVAYI
                     AGFTAPPGKTMGHAGAIVSGTAGTAQAKKEALEEHGVGVGTNPTEVARLVAESVGAPA
                     "
     gene            918..>1895
                     /locus_tag="VG709_05715"
     CDS             918..>1895
                     /locus_tag="VG709_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWC32609.1"
                     /translation="MAVISFARGIPGPDLLPVDEFGECARRATERDGARALNYGPPSG
                     YPPLREWVAERPGVDPARVVLTTGSLQGFNFVARHLVGEGATVFVEAPCYDRSLGILR
                     ALGAAARPIPLRDDGLDVDALAAALADVGGPKLVYTIPTFQNPSGRTLSRNNRARLLD
                     LARSSGALVLEDDPYGLLRFEGDPLPTLFELAGGEGVLFLSSFSKTVAPGLRVGYVVL
                     PEELVGAIEGFVLENYVSPSIFVQAALHEFVTQGFFEVNLERVRRELRLRRDAMLAAL
                     ARDLPDGARWNEPEGGYFRWRDLPRYVDAASLLDRAGDADVTFVKGADFY"
BASE COUNT          254 a          694 c          676 g          271 t
ORIGIN      
        1 caccggccgc gagggcacct tccacgcgat gaacaaccgc cgctacggca cggacgtcgt
       61 cgcgggcgtc accccgggca agggcggcca ggacgtcgag ggcgtgtcgg tcttcgacac
      121 cgtgcacgag gcggtgcgcg aggcgggcgc caacaccgcg atggtgttcg tgccgccgcg
      181 cttcgccgcc gactcgatcc tcgaggcggc cgacgccggc gtgtcgctcg tggtggccat
      241 caccgagggc atccccgccc acgacatgct gcgcgtctac aaccacatcc gcccccgggg
      301 cgtcacgctg atcggaccga actgccccgg cgtcctctcg cccggcaagg cgaacgtcgg
      361 aatcatcccc gccgagatct tcggcgaggg cggcgtgggg ctcgtcagcc gctcgggcac
      421 gctcacgtac cagatcggcc acgagctgac gcagctcggg atcggccagt caacgatcgt
      481 cgggatcggc ggcgacccca tcgtcggctc gtcgttcgtg gacgtgctcg cgaagttcca
      541 gggcgacccc gagacggagg tcgtcgtcat ggtcggcgag atcggcggcg cggaggagga
      601 gaaggcggcc gcggtcatcg acgcggagat gtcgaagccg gtcgtcgcct acatcgcggg
      661 cttcaccgcc ccgccgggca agacgatggg gcacgccggc gcgatcgtct cgggcacggc
      721 ggggacggcg caggcgaaga aggaagcgct cgaggagcac ggcgtcgggg tgggtacgaa
      781 cccgaccgag gtcgcccggc tcgtcgccga gtccgtcggc gcccccgcgt agcgcgacgc
      841 gccggcgcgc cgcgcgacgc ggcgacagga aggaaaacgc gcaggagcaa gccgcgcgcg
      901 gcactagact ccggcgcgtg gccgtcatct ccttcgcgcg cgggattccc gggccggacc
      961 tcctgccggt ggacgagttc ggcgagtgcg cgcggcgcgc gaccgagcgc gacggggctc
     1021 gcgccctgaa ctacgggcct ccctccggct acccgccgct tcgcgagtgg gtcgccgagc
     1081 gccccggcgt cgatcccgcg cgtgtcgtcc tgaccacggg gtcgctgcag gggttcaact
     1141 tcgtcgcgcg ccacctcgtc ggcgaagggg caaccgtgtt cgtcgaggcg ccgtgctacg
     1201 accgctcgct cgggatcctc cgcgcgctcg gcgcggctgc ccgcccgatt ccgctgcgcg
     1261 acgacgggct cgacgtcgac gcgctcgccg ccgcgctcgc ggacgtgggc ggccccaagc
     1321 tcgtctacac gatcccgacc ttccagaacc cgagcgggcg cacgctctcg cgcaacaacc
     1381 gcgcgcggct gctcgatctc gcccggtcgt ccggcgcgct cgtcctcgag gacgacccct
     1441 acggccttct ccgcttcgag ggcgatccgc tgccgacgct cttcgagctg gcgggaggtg
     1501 aaggcgtcct cttcctgagc tccttctcga agactgttgc tcccggcctc cgcgtcggct
     1561 acgtcgtcct cccggaagag ctcgtggggg cgatcgaggg attcgtgctc gagaactacg
     1621 tctctccgtc catcttcgtg caggccgcgc tccacgagtt cgtgacgcag gggttcttcg
     1681 aggtgaacct ggagcgggtt cgccgggagt tgcgtcttcg gcgagacgcg atgctcgccg
     1741 cgctcgcgcg cgacctcccc gacggcgcgc ggtggaacga acccgagggc ggctacttcc
     1801 gctggcgcga cctgccccgc tacgtcgacg cggcgtcgct cctcgaccgt gccggcgacg
     1861 cggacgtcac gttcgtcaag ggcgcggact tctac
//
LOCUS       DASZOB010000358         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98456, whole genome shotgun sequence.
ACCESSION   DASZOB010000358 DASZOB010000000
VERSION     DASZOB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98456"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1854)
                     /locus_tag="VG709_05720"
     CDS             complement(<1..>1854)
                     /locus_tag="VG709_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWC32610.1"
                     /translation="GEFAYSSDLWAAGMLWGHTVRSPHAHARIVSVDVSAAVAMPGVH
                     AVLTHEDVPGKKLYGLEFADQPVLAIERVRYFGEPVVLVAAEHPEQARRAAEKVEVEY
                     EPLEPVVDMERAFDEEELHPERPTEEAGYYPDPRRNVVRTIVIRHGDPDAEGDVSVSG
                     VYELGIQDQAFLGPESGLAVPDGEGGVDVYVATQWLHVDRAQVAPCLGLTPEQVRIHL
                     AGVGGAFGGREDLSMQIHAAMLALHTDRPVKMVYGRQESFVGHVHRHPARIRAEHRAA
                     SDGRLVCVRMRILLDGGAYASSSTAVTSNAASFACGPYAVPNALIESTCVYTNNPPCG
                     AMRGFGAVQSCFAHEAQMDKLAAELGVDPVELRLRNAIASGDTMPNGQRVTGSLPVAD
                     VIRRCAALPVPEPEPLPRDAIRLPGGSGNVTRGEGVKRGVGFAVGFKNVCYSHGCDDY
                     CAARVRLFADAGGELVAEVHSASVDVGQGVTDVIAQVARTELELENVVLAPHTTASVD
                     SAGSASASRMTWMASGAVALACRAVREELERRGGSLTAGEEIDVERIYRHPQTWPLDP
                     DTGQVQGERAHVAFICSAMRAVVEVDTELGLTRVVWIGAAQEVGKAVNPLAV"
BASE COUNT          257 a          683 c          667 g          247 t
ORIGIN      
        1 cacggccagg gggttcacag ccttgccgac ctcctgggcg gcgccgatcc agacgacgcg
       61 cgtgagcccg agctcggtgt cgacctcgac cactgcgcgc atcgccgagc agatgaacgc
      121 cacgtgggcg cgctcgccct ggacctgccc ggtgtcgggg tcgagcggcc acgtctgcgg
      181 gtggcggtag atccgttcca cgtcgatctc ctcgccggcc gtgagcgagc cgccccgccg
      241 ctcgagctcc tcccgcacgg cacggcaggc gagcgccacg gcacccgatg ccatccaggt
      301 catccgtgag gccgaggccg atccggcgga gtcgacggac gccgtcgtgt gcggcgcgag
      361 cacgacgttc tcgagctcga gctccgtgcg ggcgacctgc gcgatcacgt cggtgacgcc
      421 ctggccgacg tcgacggatg ccgagtggac ctcggcgacg agctcgccgc cggcgtccgc
      481 gaagagacgg acgcgcgccg cgcagtagtc gtcgcagccg tgtgagtagc agacgttctt
      541 gaacccgacg gcgaagccga cgccgcgctt gacgccctcg ccgcgcgtga cgttcccgga
      601 gccgcccggg aggcggatcg cgtcccgcgg cagcggctcc ggctcgggaa cgggaagcgc
      661 ggcgcagcgg cggatcacgt cggcgaccgg gagcgacccc gtgacgcgct gcccgttcgg
      721 catcgtgtcg ccgctcgcga tggcgttccg gaggcgcagc tcgaccgggt cgacgccgag
      781 ctcggccgcg agcttgtcca tctgcgcctc gtgggcgaag cacgactgca ccgcgccgaa
      841 cccgcgcatc gcgccgcacg gcgggttgtt cgtgtacacg cacgtgctct cgatcagcgc
      901 gttcggcacg gcgtacgggc cgcacgcgaa cgacgccgcg ttcgaggtga cggcggtgga
      961 cgaggaggcg tacgcgccgc cgtcgagcag gatccgcatc cgcacgcaga cgaggcggcc
     1021 gtcgctcgcc gcgcggtgct cggcccggat ccgcgcgggg tgccggtgca cgtggccgac
     1081 gaacgactcc tggcggccgt agaccatctt caccggccgg tccgtgtgca gcgccagcat
     1141 cgccgcgtgg atctgcatcg acaggtcctc gcggccgccg aacgcgccgc cgacgccggc
     1201 caggtggatt cgaacctgct ccggagtgag cccgagacag ggcgccacct gcgcccggtc
     1261 gacatggagc cactgcgtcg cgacgtagac gtcgaccccg ccctcgccgt cgggaacggc
     1321 gagcccggac tccgggccga ggaacgcctg gtcctggatc ccgagctcgt agacgcccga
     1381 cacggagacg tcaccttcgg cgtccgggtc gccgtgccgg atcacgatcg tacgaacgac
     1441 gttgcggcgc gggtcggggt agtagcccgc ctcctcggtc ggccgctcgg gatggagctc
     1501 ctcctcgtcg aacgcgcgct ccatgtcgac cacaggctcg agcggctcgt actcgacctc
     1561 gaccttctcg gccgcccgcc gcgcctgttc gggatgctct gcggcgacga gcacgacggg
     1621 ctcgccgaag tagcggacgc gctcgatcgc gagcaccggc tggtcggcga actcgagccc
     1681 gtagagcttc ttcccgggca cgtcctcgtg cgtcaggacg gcgtggacgc ccggcatcgc
     1741 gaccgccgcc gagacgtcca ccgagacgat gcgggcatgc gcgtgcgggc tgcgaacggt
     1801 gtggccccag agcattcccg ccgcccagag gtcgctcgag tacgcgaact cgcc
//
LOCUS       DASZOB010000359         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98471, whole genome shotgun sequence.
ACCESSION   DASZOB010000359 DASZOB010000000
VERSION     DASZOB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98471"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..132)
                     /gene="rplD"
                     /locus_tag="VG709_05725"
                     /pseudo
     CDS             complement(1..132)
                     /gene="rplD"
                     /locus_tag="VG709_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160491.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            141..506
                     /gene="rplD"
                     /locus_tag="VG709_05730"
     CDS             141..506
                     /gene="rplD"
                     /locus_tag="VG709_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWC32611.1"
                     /translation="MGGGIAHGPHPRDYTMRVNKKMKRLALRSALTDRAREGRIRAVD
                     GLSFDEPSTKDAAAVLEALALEGRVLLVLPAPAEVVEKSFRNLPEARVVYPGSLSTYD
                     VLYADWVVFVGDALEAVKP"
     gene            461..790
                     /gene="rplW"
                     /locus_tag="VG709_05735"
     CDS             461..790
                     /gene="rplW"
                     /locus_tag="VG709_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWC32612.1"
                     /translation="MGGLRRRRARGGEAVKSPRDVIIRPVVSEKSYAGLEQNRYTFLV
                     SSDANKTEIKEAVQRIWNVRVTGVNTANRKGKRKRFRFTEGKRPDQKRAVVTLAEGDS
                     IEIFEQK"
     gene            798..1634
                     /gene="rplB"
                     /locus_tag="VG709_05740"
     CDS             798..1634
                     /gene="rplB"
                     /locus_tag="VG709_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWC32613.1"
                     /translation="MGLRKYKPTTPGRRGASVSDFKEITRDEPERSLVRPLPKKAGRN
                     AYGRITARRRGGGTKRQYRVIDFRREKDGVPAKVAQIEYDPNRSARIALLHYADGEKR
                     YVLAPQGLGVGDVLRSGPDAEIRAGNALPLANIPVGTVVHAIELRPGGGAKMARSAGA
                     SVQLVAKEGGSATLRLPSGEMRLVDLRCRASVGSVGNPEHELVAKGKAGRTRWAGKRP
                     KVRGVAMNPVDHPLGGGEGKSSGGRHPTTPWGKPEGRTRKKGKASDRYIARRRGKGRG
                     GR"
     gene            1634..1918
                     /gene="rpsS"
                     /locus_tag="VG709_05745"
     CDS             1634..1918
                     /gene="rpsS"
                     /locus_tag="VG709_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWC32614.1"
                     /translation="MPRSVKKGPFIDDHLIAKVEQMNQRGDKRVIRTWSRRSTIIPEM
                     VGHTIAVHDGRKHVPVFITEAMVGHKLGEFAPTRTFRTHGHHTERSTSLK"
     gene            1924..2286
                     /gene="rplV"
                     /locus_tag="VG709_05750"
     CDS             1924..2286
                     /gene="rplV"
                     /locus_tag="VG709_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018744658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWC32615.1"
                     /translation="MEARAQARFVRVSPRKARRAIDLIRGLPVSEARRVLSFSSLGAS
                     VPVKKVLDSASANAEGRAGVVADNLVVRSAGVDEGPTLRRYRPRALGRATRIRKRTSH
                     ITVVVETVAEVSPVGTEG"
     gene            2270..>3037
                     /gene="rpsC"
                     /locus_tag="VG709_05755"
     CDS             2270..>3037
                     /gene="rpsC"
                     /locus_tag="VG709_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWC32616.1"
                     /translation="MGQKVNPYGFRLGVVYPWKSRWYAGKRDYARSLHEDIKIRDYIR
                     THLSRAGISSIDIERKGDQIQVYIRTARPGIVIGRKGAEVDRIRKDIERMTAKRVDVK
                     VEDMNQAATETRPETDAQLLAQGVAEQLQGRVSFRRAMRRAVQTAMRAGALGVRVQCG
                     GRLGGIEMSRREWYREGRVPLHTLRAKVDFGLAEAKTTFGRIGVKVWVYHGDELPAEE
                     LETERARKRALAGVAMRGTGGASTGALITDEKELAEVA"
BASE COUNT          494 a         1031 c         1127 g          385 t
ORIGIN      
        1 gcgcgatgcc gccgcccacc cacagaggcg agcggatcga cccgtggcgg gcgcgcccgg
       61 tcccctctgg cgccacggct tcttgccccc gccggcgacc tcggcgcgcg cgccacgggt
      121 cgatccgctc gcctctgtgg gtgggcggcg gcatcgcgca cgggccgcac ccgcgcgact
      181 acacgatgcg cgtgaacaag aagatgaagc gcctcgcgct gcggtccgcg ctcacggacc
      241 gcgcccgtga ggggcggatc cgcgccgtcg acggcctctc gttcgacgag cccagcacga
      301 aggacgccgc cgcggtgctc gaggcgctcg cgctcgaggg gcgcgtactg ctcgtcctgc
      361 cggcgccggc cgaggtcgtc gagaagtcgt tccggaacct ccccgaggcg agggtggtct
      421 accccggcag cctctcgacc tacgacgtgc tctacgcgga ctgggtggtc ttcgtcggcg
      481 acgcgctcga ggcggtgaag ccgtgaagag cccacgcgac gtgatcatcc ggccggtcgt
      541 gtcggagaag agctatgctg gactggagca gaaccgctac acgttcctgg tctcttcgga
      601 cgccaacaag accgagatca aggaagccgt ccagcggatc tggaacgtcc gggttaccgg
      661 cgtcaacacc gcgaaccgta aggggaagcg caagcggttc cgcttcaccg agggcaagcg
      721 acccgaccag aagcgcgccg tcgtgacgct ggccgagggc gattcgatcg agatcttcga
      781 gcagaaatag aggcagcatg ggactgagga agtacaagcc gacgacaccg ggccgccgag
      841 gggcatcggt ctccgacttc aaggagatca cccgcgatga gcccgagcgc tcgctcgtcc
      901 gcccgctccc gaagaaggcg gggcggaacg cgtacgggcg catcacggcc cgccgccgcg
      961 gtggcgggac gaagcgccag taccgcgtga tcgacttccg gcgcgagaag gacggcgtgc
     1021 cggcgaaggt ggctcagatc gagtacgacc cgaaccgcag cgcccgcatc gcgctgctcc
     1081 actacgcgga cggcgagaag cgctacgtgc tcgcgccgca gggcctgggg gtcggcgacg
     1141 tccttcgctc gggccccgac gccgagatcc gcgcgggcaa cgcgctcccg ctggcgaaca
     1201 tcccggtcgg gaccgtcgtt cacgcgatcg agctccgtcc cggcggcggc gcgaagatgg
     1261 ctcgctcggc cggcgcgtcg gtccagctcg tcgccaagga gggcgggtcc gcgacgctgc
     1321 ggctgccttc gggcgagatg cgcctcgtcg acctgcggtg tcgcgcctcg gtcgggtcgg
     1381 tcgggaaccc cgagcacgag ctcgtcgcga aggggaaggc cggccgtacg cgctgggccg
     1441 ggaagcgccc gaaggtgcgc ggcgtcgcga tgaaccccgt cgaccacccg ctcggcggcg
     1501 gcgagggcaa gtcctcgggc ggccgtcacc ccacgacgcc gtggggcaag cccgagggcc
     1561 ggacacggaa gaagggcaag gcgtccgacc ggtacatcgc ccggcgccgc ggcaagggga
     1621 ggggtggcag gtaatgccta ggtcggtcaa gaagggcccg ttcatcgacg accacctgat
     1681 cgccaaggtc gagcagatga accagcgcgg cgacaagcgc gtgatccgga cgtggtcgcg
     1741 acgctcgacg atcatccctg agatggtcgg tcacacgatc gcggtgcacg acggccgcaa
     1801 gcacgtcccc gtgttcatca ccgaggcgat ggtcgggcac aagctcggcg agttcgcgcc
     1861 gacgcggacg ttccgcacgc acggccacca caccgagcgc agcacgtcac tgaagtagga
     1921 gcgatggaag cgagagcaca ggccaggttc gttcgtgtct cgccgcgcaa ggcgcggcgg
     1981 gcgatcgatc tcatccgcgg gctgcccgtg tccgaggccc ggcgtgtgct gtcgttctcg
     2041 tcgctcggcg cctcggtgcc ggtgaagaag gtcctggatt cggcgagcgc gaacgccgag
     2101 ggccgtgccg gcgtcgtggc cgacaacctg gtggtgcgtt cggcgggcgt cgacgagggc
     2161 ccgacgctgc ggcggtaccg cccgcgcgcg ctcggccgcg ccacgaggat ccgcaagcga
     2221 acgagccaca tcacggtcgt ggtcgagacc gtcgcggagg tgagcccggt tgggacagaa
     2281 ggttaatccg tacgggttcc ggctcggcgt cgtgtacccg tggaagtcgc gctggtacgc
     2341 gggcaagcgt gactacgctc gctccctgca cgaggacatc aagatccgcg actacatccg
     2401 cacgcacctc tcgcgcgccg ggatctcgag catcgacatc gagcggaagg gcgaccagat
     2461 ccaggtctac atccgcaccg cccgtcccgg gatcgtgatc ggccgcaagg gggccgaggt
     2521 cgaccgcatc cgcaaggaca tcgagcggat gacggccaag cgcgtcgacg tgaaggtcga
     2581 ggacatgaac caggccgcga ccgagacgcg gcccgagacc gacgcccagc tcctcgcgca
     2641 gggcgtcgcg gagcagcttc agggcagggt gtcgttccgc cgcgcgatgc gccgcgccgt
     2701 gcagacggcc atgcgggccg gcgccctggg cgtccgcgtc cagtgcggcg gccgcctcgg
     2761 cgggatcgag atgtcgcgcc gcgagtggta ccgcgaaggg cgggtgccgc tgcatacgct
     2821 gcgcgcgaag gtggacttcg gactggccga ggccaagacg acgttcggcc gcatcggcgt
     2881 gaaggtgtgg gtctaccacg gcgacgagct gccggccgag gagctcgaga ccgagcgcgc
     2941 gcgcaagcgg gcgttggccg gcgtcgcgat gcgtgggacc ggcggcgcgt cgaccggggc
     3001 gctcatcacc gacgagaagg agctcgcgga ggtggcc
//
LOCUS       DASZOB010000360         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_8088,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000360 DASZOB010000000
VERSION     DASZOB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8088"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VG709_05760"
     CDS             complement(<1..194)
                     /locus_tag="VG709_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32617.1"
                     /translation="MPRRGGLVYLALVLTVAGCTGDTRPEPSDGPRPLAASGEPLERG
                     TYVTSEFSEPFAFSVGDDPW"
     gene            213..1136
                     /locus_tag="VG709_05765"
     CDS             213..1136
                     /locus_tag="VG709_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWC32618.1"
                     /translation="MAEEHYYGGQAVLEGVMMRGREHWAIAVRKPDGELHLEAHDIDS
                     VAKRYRILRKPGFRGVIALGQALAIGVKALTVSANQSAAEEEKLTPRQMALSMTFALL
                     MFVGLFFVLPAYLSSFARARVESNFVVNIGEGVFRLMLFLGYLALIGRMREIRRVFQY
                     HGAEHKTIAAFEHGEPLEPENVDRYSTLHVRCGTNFLLIVMIIAILVYSVYRPPTLGW
                     LIASRVIGIPLIAGVSFEMLRLGARHSKSRVMRALMAPGLWLQKITTKPPSDDQIEVA
                     IAAFREVRRQEDEAKAPAPAESPIPPPAEDR"
     gene            1161..2231
                     /gene="prfA"
                     /locus_tag="VG709_05770"
     CDS             1161..2231
                     /gene="prfA"
                     /locus_tag="VG709_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWC32619.1"
                     /translation="MQQRLDEIVATYEEITRALSSPDVASDAARLRELGKRHRELDQI
                     VSTYRKYRRALADAEEARALARDEGDHETASYFREEAERAAAEARELESKLELLLLPK
                     DPNDEKNVVLEIRAGAGGQEAALWAGDLFEMYRRYAERQRWKTEVLSSSPSDVGGFKE
                     VVLEVKGDRAYSRLKHESGVHRVQRIPETESGGRIHTSTATVAVLPEAEDVDVDVRDE
                     DVEVQVYRSSGPGGQSVNTTDSAVRLVHKPTGIKIEVQEERSQRQNKEKAWRYLRARL
                     LKLEQERQQQEEAAARKSQVGTGERSEKIRTYNFPQTRVTDHRIPLTSHRLEGILREG
                     ELDEFVDALMAKDRSDQLAATS"
     gene            2239..3072
                     /gene="prmC"
                     /locus_tag="VG709_05775"
     CDS             2239..3072
                     /gene="prmC"
                     /locus_tag="VG709_05775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWC32620.1"
                     /translation="MRPSDVLSRAADYLERHGVESPWETVEALLMYFLGTDRAGLYSR
                     TDGLDTRTAKLFGRALCQRCHGVPLQYLTGEQQFMDLVLGVAPGVFVPRPETEVLVER
                     ALETVEGVADPVVVDVGTGTGAIALAMKRQRPHATVLATDVSPDAVAVARANASRLAL
                     DVDVLEGDLLSPLADDIRGRVDLVVSNPPYVTREEYDELPDEVRAEPYEALVGGTDVH
                     RRLAEESPPWLRPGGWLVVEIGATQADDVRRLLDDRYERVDVHPDLAGRDRVARGRLR
                     G"
BASE COUNT          482 a         1074 c         1117 g          430 t
ORIGIN      
        1 atccaggggt cgtcgcccac gctgaacgcg aacggctcgg agaattcgct cgtcacgtag
       61 gtcccgcgct cgagcggctc tccgctcgcc gcgagcggac gggggccgtc tgacggttcc
      121 ggcctcgtat cgccggtaca cccggcgacg gtgaggacga gcgccaggta gacgaggccg
      181 ccgcgacgag gcatgtacct agaatgaggc gcgtggcgga ggaacactac tacgggggtc
      241 aggccgtcct cgagggcgtg atgatgcgcg gccgcgagca ctgggcgatc gcggtccgca
      301 agcccgacgg agagctccac ctcgaggccc acgacatcga ctcggtcgcg aagcgctacc
      361 ggatcctgcg gaagcccggg ttccgagggg tcatcgcgct cgggcaggca ctcgcgatcg
      421 gcgtcaaggc gctcacggtc tcggcgaacc agtccgccgc ggaggaggag aagctgacgc
      481 cgcgacagat ggcgctctcg atgacgttcg cgttgctgat gttcgtcgga ctgttcttcg
      541 tgctgcccgc gtacctctcg agcttcgcgc gcgcgcgcgt cgagtcgaac ttcgtcgtga
      601 acatcggcga gggtgtcttc cgcctcatgc tgttcctcgg gtatctcgcg ctgatcgggc
      661 ggatgcgcga gatccgccgc gtgttccagt accacggcgc cgagcacaag acgatcgcgg
      721 cgttcgagca cggcgagccg ctcgagccgg agaacgtcga ccgctactcg acgctgcacg
      781 tccggtgcgg gaccaacttc ctgctgatcg tgatgatcat cgcgatcctc gtgtactcgg
      841 tgtaccggcc gccgacgctc ggctggctga tcgcgtcgcg cgtgatcggc atcccgttga
      901 tcgccggcgt ctcgttcgag atgctgcgcc tcggcgctcg gcactcgaag agccgcgtga
      961 tgcgggcgct gatggcgccg ggcctctggc tgcagaagat cacgacgaag ccgccgagcg
     1021 acgaccagat cgaggtcgcg atcgcagcgt tccgcgaggt ccgccgccag gaggacgagg
     1081 cgaaggcccc cgcgccggcg gagtcgccga tcccgccgcc cgccgaggat cgctagcacc
     1141 tcggcgcgat aatggctgcg atgcagcagc gcctcgacga gatcgtcgcg acctacgagg
     1201 agatcacgcg ggccctctcc tcccccgacg tggcgtccga cgccgcgcgc ctgcgcgagc
     1261 tcggcaagcg ccaccgcgag ctcgaccaga tcgtctcgac gtaccggaag tacagacggg
     1321 cgctcgccga cgccgaggag gcacgcgccc tcgcgcgcga cgagggcgat cacgagacgg
     1381 cgtcctactt ccgggaggag gcggaacgtg ccgccgcgga ggcgcgcgag ctcgagtcga
     1441 agctcgagct gctgctgctg cccaaggacc ccaacgacga gaagaacgtc gtcctcgaga
     1501 tccgcgccgg cgccggggga caggaggccg cgctgtgggc cggcgacctc ttcgagatgt
     1561 accggcgata cgcggagcga cagcggtgga agacggaggt cctgtcgtcg agcccctcgg
     1621 acgtcggcgg cttcaaggag gtggtcctcg aggtcaaggg cgaccgcgcg tactcgcggc
     1681 tgaaacatga gtcgggcgtt caccgcgtcc agcggatccc cgagacggag tcgggcgggc
     1741 gcatccacac ctcgacggcg acggtcgcgg tgcttcccga ggccgaggac gtcgacgtcg
     1801 acgtgcgcga cgaggacgtc gaggtgcagg tctaccgctc atccggtccc ggcgggcagt
     1861 cggtgaacac gacggactcc gcggtgcggc tcgtccacaa gccgaccggc atcaagatcg
     1921 aggtccagga ggagcggtcg cagcgccaga acaaggagaa ggcgtggcgg tacctgcgcg
     1981 cacggctgct gaagctcgag caggagcggc agcagcagga ggaggccgcg gcacggaagt
     2041 cgcaggtagg gaccggcgag cggtccgaga agatccgtac ctacaacttt ccgcagacgc
     2101 gcgtgaccga ccaccgcatc ccgctcacgt cgcaccgcct cgaggggatc ctgcgcgagg
     2161 gcgagctcga cgagttcgtc gacgcgctca tggcgaagga ccgctccgac cagctcgccg
     2221 ccacgagctg acggatccgt gcgcccgtcc gacgtcctct cgcgcgcggc cgattatctc
     2281 gagcggcacg gggtggagtc gccctgggag accgtggagg cgctcctcat gtacttcctc
     2341 gggacggacc gcgccgggct ctactcgcgg accgacgggc tcgacacgcg caccgcgaag
     2401 ctcttcgggc gcgcgctctg tcagcggtgc cacggcgtgc cgctccagta cctgaccggc
     2461 gagcagcagt tcatggatct cgtgctcggg gtcgcgccgg gcgtgttcgt cccccgcccc
     2521 gagaccgagg tgctcgtcga gcgggcgctc gagacggtcg agggggtcgc cgaccccgtc
     2581 gtcgtcgacg tcggcaccgg gacgggcgcg atcgcgctcg cgatgaagcg gcagcgcccg
     2641 cacgcgaccg tgctcgcgac cgacgtctcc cccgatgccg tcgccgtggc gcgcgccaac
     2701 gcctcgcggc tcgcgctcga cgtcgacgtg ctcgagggcg acctcttatc gccgctcgcc
     2761 gacgacatcc gcggacgcgt cgacctcgtc gtcagcaacc cgccgtacgt gacgcgcgag
     2821 gagtacgacg agctgccgga cgaggtccgc gccgagccgt acgaggcgct ggtcggcggc
     2881 accgacgtgc accgccggct cgcggaggag tcgcccccat ggcttcgccc cggagggtgg
     2941 ctcgtcgtcg agatcggcgc gacgcaggcc gacgatgtgc ggcgcctgct cgacgaccgc
     3001 tacgagcgcg tcgacgtcca tcccgacctc gcggggcggg accgcgtcgc gcgcgggagg
     3061 ctccgtggct gaacggttcg atctcgacgg cccggatccg cac
//
LOCUS       DASZOB010000361         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_88479, whole genome shotgun sequence.
ACCESSION   DASZOB010000361 DASZOB010000000
VERSION     DASZOB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88479"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /gene="metK"
                     /locus_tag="VG709_05780"
     CDS             <1..692
                     /gene="metK"
                     /locus_tag="VG709_05780"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWC32621.1"
                     /translation="VIPYLRPDGKVQVTVGYRDGRPATLDTVVCSTQHPPNVDLETLL
                     KPDLVQHVIAPLIPPELDAEGLKILVNPTGMFEIGGPRADTGLTGRKIIIDTYGGMAR
                     HGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLAERFEMQVAYAIGVAHPVSVSFDT
                     FGTETVDPAKISSAIEEVFDLRPAAIIRALDLRRPIYRNTAAYGHFGRTEKDFTWERT
                     DRADDLRRLCG"
     gene            739..>2193
                     /locus_tag="VG709_05785"
     CDS             739..>2193
                     /locus_tag="VG709_05785"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32622.1"
                     /translation="MPLVAAWGVDRTFDYSISSKLEGAVRVGSLVRVPFGGRKVRGIV
                     VDVAVRVAEREVEPIVNVSFARPVAPAPLPELYEWLARRYAVPRGRAYERAVPPRVRV
                     ASPPIVPLGSATSRSRLESYEGGAELLRAISAGVGGGWCLRPYPSDGRGELIAELVGA
                     AGAAGGGAALVAVPGVHYGSQVIEALQRAFPSLVRVDSSVDDMQRSAAWSALAGGHGL
                     AAGGRGVVFAPAPDLRLIVIDEEHDSVYKEDRSPRHDARRVAMERARLQGAAVVFVSP
                     APSVETGAAAASGAIGSVHPSREAQRAGRPVVEVTAPPPDGGLSPDLHARIRDTLRNG
                     ASAALLVPAPGYARTLWCAGCRRSVRCPRCEAAVSFELQPRRVRCRRCGFTSAPPDTC
                     PSCGAREFRYLGRGAERYADQLARSFPRAPVFHMDRRAAEAGRDAITRWEGAGIYATT
                     WFGTKPELRPPVSLVGVLDADALTRRADFRAAEQA"
BASE COUNT          291 a          768 c          773 g          361 t
ORIGIN      
        1 gcgtgatccc gtacctgcgt ccggacggca aggtgcaggt aacggtgggg tatcgcgacg
       61 gccgccccgc gactttggac accgtcgttt gctcgacgca gcacccgccc aacgtcgacc
      121 tcgagacgct gttgaagccc gacctcgtcc agcacgtgat cgctcccttg atcccaccgg
      181 agctggatgc cgagggcctc aagatcctgg tcaacccaac cggcatgttc gagatcggcg
      241 gaccgcgcgc cgataccggc ctcaccggac gcaagatcat catcgacacc tacggcggca
      301 tggcgcgaca cggcggcggt gcgttctccg gtaaggaccc gacaaaggtg gaccggtccg
      361 ccgcctacgc ggcgcgctac gtcgcgaaga acgttgtcgc ggccggactt gccgagcgtt
      421 tcgagatgca ggttgcgtac gcgatcggcg tcgcgcatcc ggtttcggtc tcgttcgaca
      481 ccttcgggac cgagacggtc gatccggcga agatctccag cgcgatcgaa gaggtgttcg
      541 atcttcgacc cgctgccatc atccgcgcgc tcgatctccg ccggccgatc tatcgcaaca
      601 cggccgccta cggccacttc ggccgtaccg agaaggactt cacgtgggag cgcaccgacc
      661 gcgccgatga cctccgccgc ctctgcggat aacgaggtcc ctccctcaaa cgacgaatcg
      721 caactcctgg cggcggtcgt gccgctggtc gcggcctggg gcgtcgatcg caccttcgac
      781 tactcgatct cgtccaagct cgagggcgcc gtgagggtcg ggtcgctggt ccgggtcccg
      841 ttcggagggc gcaaggtccg cgggatcgtc gtcgacgtgg cggtgcgtgt cgccgagcgg
      901 gaggtcgagc cgatcgtcaa cgtgtcgttc gctcgacccg tcgcgccggc tcctcttcca
      961 gagctctacg agtggctggc gcgccgctac gcggtcccga ggggaagggc ctacgagcgt
     1021 gcggttccgc cgcgggtccg cgtggcgtct ccgccgatcg tgcctctcgg ctccgcgaca
     1081 tcgaggtcgc gtctcgagag ttacgagggc ggcgccgagc tgctgcgcgc gatctcggcc
     1141 ggggtcgggg gcggctggtg cttgcgcccg tatccgagcg atggccgtgg cgagctcatc
     1201 gcagagctgg tcggcgccgc gggagccgcc ggtgggggag ccgctctcgt cgccgttccg
     1261 ggcgtccact acgggtcgca ggtgatcgag gcgttgcagc gggcgtttcc gtccctcgtc
     1321 cgcgtcgaca gctcggtcga cgacatgcag cgctctgctg cgtggtccgc tctggccggt
     1381 ggacacggct tggccgcggg aggccgaggt gtggtgttcg ctccggcccc ggacctacgg
     1441 ctgatcgtga tcgacgaaga gcacgactcc gtttacaagg aggaccgctc gccgcgccac
     1501 gacgcgcgcc gggtcgcgat ggagcgagcg cggctccagg gcgccgccgt agtgttcgta
     1561 agcccggccc cttcggtgga gaccggagcc gccgcggcct ccggcgccat cggatccgtt
     1621 cacccttccc gagaggcgca gcgcgccggt cgtcccgtcg tcgaggtaac ggcacctccg
     1681 cccgacggcg gtctgtctcc cgatctgcac gcgaggatcc gagacacctt gcgcaacggg
     1741 gcttcggccg cgctgttggt tcccgctccc gggtacgcgc ggacgctgtg gtgcgccggc
     1801 tgccgccggt cggtgcggtg cccccgctgc gaagccgcgg tctcgttcga gctccagccg
     1861 cggcgggtga gatgccggcg gtgcggcttc acctcggctc ctcccgatac ctgtccttcg
     1921 tgcggcgcga gggagttccg ctatctcggt cgcggggcag agcgctacgc cgaccagctg
     1981 gcgcgttcct tcccacgagc gccggtgttc catatggatc gccgcgccgc ggaagccggc
     2041 cgcgacgcga tcacgaggtg ggagggagcc gggatctacg ccacgacctg gttcggcacg
     2101 aaacccgagc tccgaccgcc cgtctcgctg gtcggcgtgc tcgacgccga cgcgttgacg
     2161 cgacgcgcgg atttccgcgc cgcggagcag gcg
//
LOCUS       DASZOB010000362         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_13667, whole genome shotgun sequence.
ACCESSION   DASZOB010000362 DASZOB010000000
VERSION     DASZOB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13667"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1660
                     /gene="typA"
                     /locus_tag="VG709_05790"
     CDS             <1..>1660
                     /gene="typA"
                     /locus_tag="VG709_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWC32623.1"
                     /translation="LAKNTAVRHGGRKINIVDTPGHADFGGEVERGLHMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEVRLPVILVLNKVDRADARIEEAVNEVYELFLDLDADESQI
                     EFPIVYCNARAGRAGLAADELEPDLEPLFETLVAHIPAPSYEDGHPLQAHVTNLDASP
                     YVGRLALCRVREGTIHKGQQVAWCRADGSIERAKVTELYVTEALDRVVAEEAGPGELN
                     AVAGMPGVTIGETLADPEDTRPLPVIGVDEPSLAMTVGINTSPLSGRDGDRLTAGMIE
                     ARLEQELVGNVSLRVLPTERPDAWEVQGRGELQLAVLVELMRREGCELTVGKPQVVTR
                     EIEGKVHEPVERLAIDVPDEYVGVITQLLALRKGRLEQMVNHGTGWVRMDYLAPARGL
                     IGFRTEFMTETRGTGILHHVFDGYEPWHGELRTRPTGSLVADRRGSTTSFALLNLQER
                     GQLFVAPGVEVYEGMIVGVNARVEDLDVNATKEKKLTNMRSSTADELVRLIPPRPLSL
                     EQALELIRDDECVEVTPHHIRLRKLELSAQKRQTAASRRARGLVA"
BASE COUNT          260 a          579 c          595 g          226 t
ORIGIN      
        1 ctcgccaaga acaccgccgt ccgccacggc ggacgcaaga tcaacatcgt cgacaccccc
       61 gggcacgccg acttcggggg agaggtcgaa cgcggtctgc acatggtcga cggagtgctc
      121 ctgctcgtgg acgcgagcga gggaccgctc ccgcagaccc ggttcgtgct gcgcaaggcg
      181 ctcgaggtgc ggctgccggt catcctcgtc ctcaacaagg tcgaccgggc ggacgcgcga
      241 atcgaggagg cggtgaacga ggtctacgag ctcttcctcg acctcgatgc cgacgagagc
      301 cagatcgagt tcccgatcgt ctattgcaac gcgagagcgg gacgggcggg ccttgccgcc
      361 gacgagctcg agcccgatct ggagccgctc ttcgagacgc tggtcgcgca cattccggcg
      421 ccgtcctacg aggacggcca cccgctgcag gcccacgtca cgaacctcga cgcctcgccg
      481 tacgtcggcc ggttggccct ctgccgcgtt cgcgagggca cgatccacaa ggggcagcag
      541 gtcgcgtggt gccgcgccga cggcagcatc gagcgcgcta aggtgacgga gctctacgtg
      601 accgaggcgc tcgaccgggt cgtcgctgag gaggcgggcc cgggcgagct gaacgccgtc
      661 gcgggcatgc ccggggtgac gatcggcgag acgctcgcgg atcccgagga cacgcggccg
      721 ctgccggtga tcggcgtcga cgagccgagc ctcgcgatga ccgtcgggat caacacctcg
      781 ccgctctccg ggcgggacgg cgaccggctg accgccggca tgatcgaggc gcggctcgag
      841 caggagctcg tcggcaacgt ctcgctgcgc gtgctcccga ccgagcgccc cgacgcgtgg
      901 gaggtgcagg gccgcggcga gctccagctg gccgtgctcg tcgagctcat gcgccgcgag
      961 ggctgcgagc tcacggtcgg caagccgcag gtggtgacgc gcgagatcga gggcaaggtt
     1021 cacgagcccg tcgagcgcct cgcgatcgac gtacccgacg agtacgtcgg cgtgatcacg
     1081 cagctcctgg cgcttcggaa gggccgcctc gagcagatgg tgaaccacgg cacgggctgg
     1141 gtgcggatgg actacctggc gccggcgcgc ggcctgatcg gcttccgcac cgagttcatg
     1201 accgagacgc gcggcaccgg catcctccac cacgtcttcg acgggtacga gccctggcac
     1261 ggcgagctcc gcacgcggcc taccggctcg ctggtcgccg accggcgcgg ctcgacgacg
     1321 tccttcgcgc tgctcaacct ccaggagcgc ggccagctct tcgtcgcgcc gggcgtggag
     1381 gtctacgagg ggatgatcgt cggcgtgaac gcgcgcgtcg aggaccttga cgtcaacgcg
     1441 acgaaggaga agaagctgac gaacatgcgc tcctcgacgg ccgacgagct cgtccgcctg
     1501 atcccgccgc ggcctctctc gctcgagcag gcgctcgagc tgatccgcga cgacgagtgc
     1561 gtggaggtga cgccgcacca cattcgcctc cgcaagctcg agctctccgc gcagaagcgt
     1621 cagacggcgg cgtcgcggag ggctcgcggt ctcgtcgcgc
//
LOCUS       DASZOB010000363         4999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_13672, whole genome shotgun sequence.
ACCESSION   DASZOB010000363 DASZOB010000000
VERSION     DASZOB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13672"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VG709_05795"
     CDS             complement(<1..507)
                     /locus_tag="VG709_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWC32624.1"
                     /translation="MRLALAGDTMLGRGVAERLSEASPESLFAPEVVDAARDADAFVL
                     NLECCISERGEPWPDPRKPFFFRAPPAAVAALQHLGVDCVTLANNHALDFGAVALEDT
                     LRLLAEGGIGAAGAGADVEAARAPAVFDVAGERLAVLGFTDHPRDFAAAPDGPGVAFA
                     DLRGEGLPS"
     gene            complement(528..725)
                     /locus_tag="VG709_05800"
     CDS             complement(528..725)
                     /locus_tag="VG709_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32625.1"
                     /translation="MTKPQQPELRRSDRGQTDQGEWARREEARRRRRERDPSGPVPEA
                     NQPGHRPDEEQDRPEAPPSER"
     gene            827..1528
                     /locus_tag="VG709_05805"
     CDS             827..1528
                     /locus_tag="VG709_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32626.1"
                     /translation="MRPEGRSPEDRARIAEGRPLDAAMSALGVVFLLVVVGEGLATRG
                     TPLSRGFAIAGWGLWAIFVGEFIYRLVVAPDTGRFLKRNWWQIVFLVLPFLRIFRLLH
                     TLRVARAGRVLGSAVRAGRSAGRKLTDRVAWLSTLTAIVILASSQLLFEFGELGSYAT
                     ALHAAALSSVTGEPIQGGGTITRVVEIVLAIYSVVVFAALAGILGAYFLERRDATPSA
                     RITQAAGDREPRRAG"
     gene            complement(1535..2581)
                     /locus_tag="VG709_05810"
     CDS             complement(1535..2581)
                     /locus_tag="VG709_05810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWC32627.1"
                     /translation="MRLDQIGDVLMTTPAIRALRESAPGRHVTLLAAPSGAEVARLAP
                     EIDDVVVYDAPWMKASPARGDRSRDRAIVDGLRERRFDAAVVFTVYSQSPLPAAYLCY
                     LAGIPRVLAHVRENPYQLVTDWARETEPQREVRHEVRRQLDLVSTVGASTRDETLSLD
                     VPQAARRRAAAAIAGIDEACPWAVVHPGASAPSRRYPAEHYVEVCERLVRDHGWQIVF
                     SGAPDEVDLVESIRASMTSTSSSLAGTLDVATLAAVLELAPVLIAGNSGPVHIAAAVG
                     TPVVDLYALTNPQHTPWGVEHEVLSHDVPCKYCYSSVCPMGHHDCLRLVRPSDVVDAA
                     MRLGARTAARRAPM"
     gene            2741..4393
                     /locus_tag="VG709_05815"
     CDS             2741..4393
                     /locus_tag="VG709_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWC32628.1"
                     /translation="MARILGINAIFHDPAAAVVVDGEIVAAAEEERFSRRKHGKRPLP
                     FSAWELPEQAARWCLDAAALAPEDLDAVTYSYDPDLAVATATDIVEDDWEGLRTLYAR
                     RAPLFLRTYLPGLDPGVVRFLPHHVAHAASAYLAAPFPDSAVMVHDGRGERTAYIAGR
                     AQRGDLEVLATQDLPHSLGIMYEEATEHLGFKRSSDEYKVMALAAYGEPSFLSEMRRA
                     IRVTDDGRFEVDPIEWSAFAKRRTGEEWDRGHADLAASVQRRLEEVLLELARWLHGVT
                     GERSLTMAGGVALNCVANTRLLDEGPFDRIWVQPAAGDAGTALGGALYTAHSLGDRTE
                     TMTTAALGRGWSDDELEAWLVSAGVPYERPTDVAETVAEALVDDAIVAWFEGRSEYGP
                     RALGHRSLLADPRHAVNLERLNRVKGREQFRPVAPMVLAERAPAVFEGGPFPSPYMLF
                     THRVRREWRERIPAVVHVDGTARIQTVDAGEAPLVARMISAFERRTGVPVVVNTSLNT
                     AGRPMVDDPRDALECFGSAPVDLLAIGPFVVRRASFFAGRDR"
     gene            4306..4935
                     /locus_tag="VG709_05820"
     CDS             4306..4935
                     /locus_tag="VG709_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HWC32629.1"
                     /translation="MLRLRSGGPPRDRAVRRSASVVLRGTRSMSDRPSAVLFDRDGTI
                     VVDVPYNGDPERVAPVPGAREAIDRLRSAGIPVGIVTNQSAVGRRLITEEQLDAVNRR
                     VEELLGPFAVWAICPHAPDAGCACRKPEPQLVRDAAARRGVPAERCVVIGDTGADVEA
                     ARSAGARAILVPTDVTLEEEVRAAPEVAPSIGDAVDRLLAAATVGGARR"
BASE COUNT          696 a         1808 c         1789 g          706 t
ORIGIN      
        1 ggacggaagc ccctcccccc gcaggtcggc gaacgcgacg cccgggccgt ccggcgcggc
       61 ggcgaagtcg cgcggatgat cggtgaagcc gagcaccgcg agccgctccc cggccacgtc
      121 gaacacggca ggcgcacgag cggcctcgac gtccgctccc gcgcccgccg ccccgatgcc
      181 gccctcggcg aggagccgca gcgtgtcctc gagcgcgaca gcgccgaagt ccagcgcgtg
      241 gttgttcgcg agcgtgacgc agtccacgcc gaggtgctga agcgccgcca cggccgcagg
      301 cggcgcccgg aagaaaaacg gtttgcgggg atccggccac ggctccccgc gctccgagat
      361 gcagcactcg agattcagga cgaacgcgtc ggcgtcacgc gcggcgtcga cgacctccgg
      421 cgcgaacagc gactcgggcg aagcctccga caggcgctcc gcgacgccgc ggccgagcat
      481 cgtgtctccc gcgagggcga gcctcacggg gccatgctag gtgccggcta ccgttcggac
      541 ggcggggcct cggggcggtc ctgctcctcg tccgggcgat gtcccggctg gttggcctcg
      601 ggcaccgggc cgctcggatc cctctcgcgc cggcgcctgc gtgcctcctc gcgtctcgcc
      661 cattccccct gatcggtctg gcctcgatcg ctccggcgga gctctggctg ctgcggcttc
      721 gtcacggcga tcctcctttc ccgcacctca ttccttcccg accgcggcgg cgcgccaaac
      781 accgcgccgt ttcccggccg acggcggagg gtatcgcggc tacgccatgc ggcccgaggg
      841 gcggtcgccc gaagaccgtg cacgcatcgc ggagggaaga ccgctcgacg ccgcgatgtc
      901 ggcgctcggc gtcgtgttcc tcctcgtggt ggtcggagag ggtctcgcga cgcgcggaac
      961 gccgctctcg cgcggcttcg cgatcgcggg atggggcctc tgggcgatct tcgtcggaga
     1021 gttcatctac cggctcgtcg tcgctcccga cacggggcgg ttcctcaaac ggaactggtg
     1081 gcagatcgtc ttcctcgtgt tgccgttcct ccggatcttc cgcctgctcc acacgctccg
     1141 cgtcgcgcgg gccggacgcg tcctcgggtc ggcggttcgc gccgggcggt ctgccggacg
     1201 gaagctcacg gaccgcgtcg cgtggctcag cacgctgacc gcgatcgtga tcctggcgtc
     1261 gagccagctg ctgttcgagt tcggcgagct cggctcgtac gccaccgcgc tacacgcggc
     1321 ggcgctttcg agcgtcacgg gagagccgat ccagggcggc ggcacgatca cacgcgtcgt
     1381 ggagatcgtg ctcgccatct actcggtcgt ggtgttcgcc gcgctggccg ggatactcgg
     1441 cgcgtacttc ctcgaacgac gcgacgcgac gccctcggcc cggatcacgc aagccgccgg
     1501 cgatcgtgaa cctcgccggg ccggctgaac gccgtcacat cggcgcgcga cgcgcggccg
     1561 tccgagcgcc gagccgcatc gccgcgtcca cgacgtccga tggacggacg agccgcaggc
     1621 agtcgtggtg ccccatcgga cacacgctgc tgtagcagta tttgcagggc acgtcgtgcg
     1681 agagcacctc gtgctcgacg ccccagggcg tgtgctgcgg gttcgtgagc gcgtagaggt
     1741 caacgaccgg cgtcccgacg gccgcggcga tgtggacggg gccgctgttc ccggctatca
     1801 ggaccggtgc gagctcgagc accgccgcga gcgtcgcgac gtcgagggtc cccgccagcg
     1861 acgacgacgt ggaggtcatc gacgcacgga tcgactcgac gaggtcgacc tcgtccggag
     1921 cgcccgagaa cacgatctgc catccgtgat cccggacgag ccgctcgcag acctcgacgt
     1981 aatgctctgc ggggtaccgc cgagacggcg ccgaggcccc gggatgaacg accgcccacg
     2041 ggcacgcctc gtcgatgccg gcgatcgccg cggccgcacg gcgccgtgct gcttgcggaa
     2101 cgtcgagcga gagcgtctcg tcgcgagtcg atgcgccgac ggtcgagacg agatcgagct
     2161 gccggcgaac ttcgtgccga acctcgcgct gcggttccgt ctcgcgcgcc cagtccgtga
     2221 cgagctggta cgggttctca cgcacgtgcg cgagcacgcg cgggatcccc gcgagatagc
     2281 acaggtacgc ggcgggcagc gggctctgcg agtacacggt gaagacgacg gcggcgtcga
     2341 accggcgctc ccggagcccg tcgacgatcg cgcgatcgcg cgagcgatcg ccgcgtgcgg
     2401 gggacgcctt catccaagga gcgtcgtaga cgacgacgtc gtcgatctcc ggtgcgagcc
     2461 gcgcgacctc ggcccccgat ggcgcggcga gcaacgtcac gtggcgtccg ggcgcgctct
     2521 cgcgaagcgc gcggatcgcc ggcgtcgtca tcagcacgtc gccgatctgg tcgagacgca
     2581 cgcaaaggag gtggcgtgcg tcgcgccagc gatcctgatc gggctccatc acccgtgcgc
     2641 ttccccgcgt cgacggcccg gcaaaccaag cttccgttta ccgagaagcg gttcgggaaa
     2701 gacggcgggg caggcgagca agcacagcag gaggtcggac ctggcgcgca tcctcggcat
     2761 caacgcgatc ttccacgatc ctgcggcggc agtcgttgtc gatggggaga tcgtcgccgc
     2821 ggccgaggag gagcgcttct cgcgccggaa gcacggcaag cgcccgctcc cgttctccgc
     2881 gtgggagctc ccggaacagg cggcgcgctg gtgtctcgat gcagctgcgc tcgcgcccga
     2941 agacctggac gcggtcactt actcgtacga cccggatctc gccgtagcga cggccacgga
     3001 catcgtcgag gacgactggg aagggcttcg gaccctctac gctcgacggg cgccgctgtt
     3061 cctgcggacg tacctccccg ggctcgaccc cggcgtggtg cggttcctcc cacaccacgt
     3121 cgcacacgcc gcctccgcgt acctcgccgc accgttcccc gactccgccg tcatggtgca
     3181 cgacggccgc ggggagcgaa ccgcgtacat cgccggtcgc gcgcagagag gtgacctcga
     3241 ggtcctggcg acgcaggatc tcccgcactc gctcgggatc atgtacgagg aggcgaccga
     3301 gcacctcggg ttcaagcgct cctccgacga gtacaaggtg atggcgctcg ccgcgtacgg
     3361 cgaaccctcg ttcctctcgg agatgcgacg cgcgatacgt gtcaccgacg acgggaggtt
     3421 cgaggtcgac ccgatcgagt ggagcgcgtt cgcgaagcgc cgcaccggcg aggagtggga
     3481 ccgggggcac gccgacctcg ccgcgagcgt gcagcgtcgt ctcgaagagg tgctgctcga
     3541 gctcgcgagg tggcttcacg gcgtcacagg cgagcgcagt ctcacgatgg ccggcggcgt
     3601 cgccctcaac tgcgtcgcga acactcggct gctcgacgag ggcccattcg accggatctg
     3661 ggtgcagccc gcagcgggag acgccggcac cgcgctcggc ggcgcgctct acaccgcgca
     3721 ctcgctcggc gaccgtacgg agacgatgac cacggccgcc ctcgggagag gctggagcga
     3781 cgacgagctg gaggcgtggc tcgtgagcgc cggcgtcccg tacgagcgcc caaccgacgt
     3841 cgcggagacc gtcgccgagg cgctggtcga cgacgcgatc gtcgcgtggt tcgagggacg
     3901 cagcgagtat ggtccacgcg ccctcgggca ccggagcctc ctggccgacc cccgtcacgc
     3961 cgtcaacctc gagcgcctca accgcgtgaa gggccgcgag cagttccgtc ccgtcgcgcc
     4021 gatggtcctc gccgaacgcg cgccggccgt gttcgagggc ggccccttcc cgagccctta
     4081 catgctgttc acgcaccgcg tccgccgtga gtggcgcgag cgcatccccg ccgtcgtcca
     4141 tgtcgacggg accgcacgca tccagaccgt cgacgcgggc gaggcgccgc tcgtggcgcg
     4201 gatgatctcg gcgttcgagc ggcggaccgg cgtccccgtc gtcgtcaaca cgagcctcaa
     4261 cacggcgggg cgtccgatgg tcgacgaccc tcgcgacgcg ctggagtgct tcggctccgc
     4321 tccggtggac ctcctcgcga tcgggccgtt cgtcgttcgg cgagcgtcgt tcttcgcggg
     4381 acgcgatcga tgagcgaccg cccttcggcc gtgctgttcg accgcgacgg gacgatcgtg
     4441 gtcgacgtcc cgtacaacgg cgatcccgag cgcgtggccc cggtgccggg cgcgcgtgag
     4501 gcaatcgacc gtctgcggtc cgccggcatc ccggtcggca tcgtcacgaa ccaaagcgcc
     4561 gtcggccgcc gcctcatcac cgaggagcag ctcgacgccg tgaaccgtcg cgtcgaggag
     4621 ctgctcgggc cgttcgcggt gtgggcgatc tgcccgcacg ctcccgacgc cggatgcgcg
     4681 tgtcgcaaac cagagccgca gctggttcgc gacgcggcgg cccgccgcgg cgtgccggcg
     4741 gagaggtgcg tcgtcatcgg cgacacgggg gccgacgtcg aggccgcgcg ctcggccggc
     4801 gctcgagcga tcctcgtgcc gacagacgtg acgctggagg aggaggtacg ggcggcgccc
     4861 gaggtggcgc cctcgatcgg tgacgccgtc gaccggctcc tcgccgccgc gaccgtcgga
     4921 ggggcgcgac gatgagggtc gcgatggtgt cggagcacgc gagcccgctc gccgtcctcg
     4981 gcggcgtcga ctcgggcgg
//
LOCUS       DASZOB010000364         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128566, whole genome shotgun sequence.
ACCESSION   DASZOB010000364 DASZOB010000000
VERSION     DASZOB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128566"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1405)
                     /locus_tag="VG709_05825"
     CDS             complement(131..1405)
                     /locus_tag="VG709_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWC32630.1"
                     /translation="MHANGAEPAGNGHRRSTGARAEHPGHRSPPRRRVRVLLAEDNPS
                     IRETLVKLLRDEPAIDLVGVAHDAPAAVDLARRVQPDVAVLDVKMPGGGGYRAAREIL
                     ASRSTTTVMALSAYEDRATVLEMVRAGAIGYALKGDPVGDIVEAIHRTAAGTGTLAPR
                     ITGGVLGTLAKQLERNARARGYRDDVERRVRHVIETGGPAIVFQPIVDLTSGHVAGFE
                     ALSRFPGPRGAGVWFSDADSVDLRQELEIAAVRRAAASHPRLPDPAYLSINVSPDVVA
                     DDRFREALGEIPAKRVVVEITEHACVADYAETARAIEPLREHGVRLAVDDAGGGFASM
                     KHVLRLSPDIVKLDVDITRDVDSDPARKALASGLLSFARDTRAELVAEGIETANELET
                     LQELGVPYGQGYRLARPAAASAPECAVRTVAV"
     gene            1400..>1627
                     /locus_tag="VG709_05830"
     CDS             1400..>1627
                     /locus_tag="VG709_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32631.1"
                     /translation="MHGITSLHRHVRAVRAVALVSGSIVFPVPRFDKSAVRGFFFGGR
                     TDGQRAIGKMTASHDATGGSAVQMDKPTQGQD"
BASE COUNT          222 a          578 c          586 g          241 t
ORIGIN      
        1 cgaccgggcg cacctcgcgc tcgaggaagg aacggaacga gtccgtcagc tcgcggtact
       61 cgtcggggat cgtgaagtcc acggcgcctc cgtggtcgac gattctaagc ggaggacgcc
      121 gtgcgagact tcagaccgcg acggtcctca cggcgcattc gggcgccgac gccgcagcgg
      181 gtcgcgccag ccgataaccc tggccgtagg ggacgccgag ctcctggagc gtctccagct
      241 cattcgccgt ctcgatcccc tcggccacga gctccgctcg cgtgtcccgc gcgaacgaga
      301 gcagccccga cgcgagcgcc ttccgcgcgg gatccgagtc gacgtcgcgc gtgatgtcga
      361 cgtccagctt cacgatgtcc ggcgagagcc gcagcacgtg cttcatgctt gcgaacccgc
      421 ctccggcatc gtcgaccgcg agccgcactc cgtgctcccg gagcggctcg atcgcccgcg
      481 ccgtctccgc gtaatcggcg acgcacgcgt gctcggtgat ctcgacgacg acacgtttcg
      541 cggggatttc gccgagcgcc tctcggaacc ggtcgtcggc gacgacgtcg ggcgagacgt
      601 tgatcgagag gtaggcggga tcggggaggc gtgggtgcga cgccgcggcc cgccggacgg
      661 ccgcgatctc gagctcctgc cggaggtcca ccgagtcggc gtcgctgaac cacacgccgg
      721 cgccgcgtgg gccggggaac ctcgacagcg cctcgaagcc cgcgacgtgg ccggaggtca
      781 ggtcgacgat cggctggaac acgatcgcgg gcccgcccgt ctcgatcacg tggcggacgc
      841 gacgttcgac gtcgtcgcgg tacccgcgcg cccgggcatt gcgctcgagc tgcttcgcga
      901 gcgtgccgag gacacctccc gttatccgcg gcgcgagcgt cccggtcccc gcggcggtgc
      961 gatggatcgc ctcgacgatg tcgccgaccg ggtcgccctt gagcgcgtac ccgatcgcgc
     1021 cggcccgaac catctcgaga accgtcgcgc ggtcctcgta ggcggacagg gccataacgg
     1081 tcgtcgtgga tcgcgacgcg aggatctccc gcgcggcgcg gtagccgccg ccgcccggca
     1141 tcttcacgtc gaggacggcg acgtccggct ggacgcggcg agcgaggtcg accgcggcgg
     1201 gggcgtcgtg cgcgacgccg accaggtcga tcgcgggctc gtcgcgcagc agcttgacga
     1261 gcgtctcgcg gatgctcggg ttgtcctcgg ccagcaggac acggacgcgc cgccggggcg
     1321 ggctccggtg tccggggtgc tcggcccgcg ctccggtgct ccggcgatgg ccgttcccgg
     1381 ccggctcggc tccgttcgcg tgcacgggat cacctcgctc caccgtcacg tccgggcggt
     1441 gagagccgtc gccctggtct ctggctccat cgtgttcccg gtgcctcggt tcgacaaatc
     1501 agccgttcgg gggttctttt ttgggggacg gaccgacgga cagcgtgcaa tcggtaagat
     1561 gacggcttcc cacgacgcca caggaggttc cgccgtgcag atggacaagc cgacgcaggg
     1621 gcaggac
//
LOCUS       DASZOB010000365         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_13695, whole genome shotgun sequence.
ACCESSION   DASZOB010000365 DASZOB010000000
VERSION     DASZOB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13695"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VG709_05835"
     CDS             <1..578
                     /locus_tag="VG709_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32632.1"
                     /translation="DMQAAGFNLAAIKQLLELVPAGAAKEMVRLERALLAPWATEQPE
                     VTSAEELRRRFPAATADDIERAVELGLLSFLQGGRVRIESPTLLRAGEEVVALGVPVE
                     ETLRVTEEVRRHAEGVATAFVELFVRHVWTPFVEGGRSPGDLPRMRQALERLRPLASS
                     SLVATFQRVMSEHVERAFGEQLERAAGEPDR"
     gene            891..2120
                     /gene="ugpC"
                     /locus_tag="VG709_05840"
     CDS             891..2120
                     /gene="ugpC"
                     /locus_tag="VG709_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWC32633.1"
                     /translation="MAEIALEDVSKVFPDGTAAVEDFDLGIRDGEFMVLVGPSGSGKT
                     TALRMVAGLENATGGTIRIGDRVVNDVPPKDRDIAMVFQNYALYPHMSVYENMAFGLK
                     LRRVSKDEIDRRVREAAQILGLGELLSRKPRNLSGGQRQRVAMGRAIVREPQAFLMDE
                     PLSNLDAKLRVQTRAEVARIQRELEVTTIYVTHDQTEAMTLGDRVAVIRKGRLQQVAP
                     PQTLYDNPVNLFVAGFIGSPAMNMVEAELVRDDGGLAVEFGGARFDVDDEALADRPRL
                     KEFEGRKLILGIRPEDMEDAEVVGDAPSDRRFSTTVELREDLGSEVLVHFSVDEPIVL
                     TEDTKELAADVGTEALESLEDSAQRDRSKFVARLSPTSKAREGQQVELFIDTRRLHFF
                     DPESGLGISDGEGKEAT"
     gene            2128..3060
                     /locus_tag="VG709_05845"
     CDS             2128..3060
                     /locus_tag="VG709_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWC32634.1"
                     /translation="MAAGARTIPARSSGPLAWVGRHRFELALIAPLVVYVTVLTIAPI
                     FDTIRISLLGEDGAFTTDNYGRIFGQQVFRQAVVNTVIVALVSLTLELTVGLAIALAL
                     NAKFFGRGFVRTVTLIPLGVPTIVSGAVMLLLFGSSGYLNSIFGFVVDLFNKIPGVEM
                     QFRGIDWTITGGLRTLFTIAIADMWKVLPVITLIFLAGLQSIPEDVYEAADVDGATKW
                     QRFSRVTLPLLIPYITIALILRAIDAFRIFELALVLSGRIEPVLGTYIWNQYAPPTSN
                     PFTSAAASIVLFGIIMVFIIIYLRVVARRGDVQL"
     gene            3057..3950
                     /locus_tag="VG709_05850"
     CDS             3057..3950
                     /locus_tag="VG709_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWC32635.1"
                     /translation="MSATPGTAVATRKTHTGPRRPRPRRDVLDKPLKVIFPIWIVVTV
                     LIVLFPLYLIFLVSFAPGIALFGERPDLFTSEPTLQWWRSVVEGGDLIPPLIKSLVVA
                     TVTTLVAIVIAAPAAYVISRLRPAVRYTIVIGLLVTRMFPEFAIGISVATRFAKLGFI
                     DDYTGVILAHLIGALPFIAWILVGTFETIPRELEEASAIDGASRLGTLIRVVMPLALP
                     GIAVAALFVWLYSWNEFVFARLLTTTQNTLPLQVFQAIDRGSKQQMATVAAVLTLPIL
                     FVVYFLQRYLRPGALTGAVKG"
     gene            3997..>4707
                     /locus_tag="VG709_05855"
     CDS             3997..>4707
                     /locus_tag="VG709_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWC32636.1"
                     /translation="MRKRHLLVATAAALSLVAAACGGAPETPGGPGDEGDLSGAEITF
                     NVSLAEEEQAGVQAVLDQFTQQTGANVKLSSVTADQMPQKLQVEVESGNNTIHLISQD
                     NYILEPLVADGLVQDVGDVEIPAEVSEALIPAQFDGTTYFLPFRPNVRVAYANTQRFE
                     EAGVSGPPTTHEEWRSMAEAFAQTAGEPKVTISFAEGSSTGVVASEWILSFGGNPLIL
                     NDEGSVAAFEFLQDAWNDG"
BASE COUNT          689 a         1581 c         1675 g          762 t
ORIGIN      
        1 gggacatgca ggcggccggg ttcaacctgg ccgcgatcaa gcagctcctc gagctcgtgc
       61 cggccggtgc ggccaaagag atggtgaggc tcgagcgggc actgctcgcg ccatgggcga
      121 ccgagcagcc ggaggtcacg tcggccgagg agctcaggcg gcggttcccg gccgcgaccg
      181 ccgacgacat cgagcgcgcc gtcgagctcg gcctcctttc gttcctccag gggggacgcg
      241 tccggatcga gagcccgacg ctgctgcgcg ccggggagga ggtcgtggcc ctcggcgtgc
      301 ccgtggagga gacgctccgc gtcaccgagg aggtgcggcg ccacgccgag ggcgtcgcga
      361 ccgcattcgt ggagctgttc gtgcggcacg tctggacgcc gttcgtggag ggcgggcgtt
      421 caccgggaga cctgccacgg atgcgtcagg cgctcgagcg gctgcggcca ctcgcgtcgt
      481 cctcgctcgt cgcgaccttc cagcgggtga tgtccgagca cgtcgagcgc gcgttcggcg
      541 agcagctcga gcgtgccgcg ggcgagcccg accgctgacg cgtacgggct cgagagtgga
      601 acgccgctag gagcggcggc gggcgcgacg cgccctgcgc cgctcgcggc gagcgtcttg
      661 ggccttggtg cggagcgcag agatcgcgcc gttccagagt gctgggacca tgtcgatcac
      721 gactcctcgt ggtcggaact ggttacggtc ggtgcaacat ccggcgggtc gccgtgattc
      781 cctccggcgc cgcctccatg tgcaccctaa caaccggcgt cgacggcccg attggcgcct
      841 tcggctctct cggggatatc gttacccgca gtcggaccgg gggtgggctc atggcggaga
      901 tcgccctaga ggacgtcagc aaggtcttcc cggatgggac ggctgccgtc gaggacttcg
      961 acctcggcat ccgggacggc gagttcatgg tcctcgtcgg gccgtccggc tccggaaaga
     1021 ccaccgccct gcggatggtc gcgggcctcg agaacgccac cggcgggacg attcggatcg
     1081 gcgaccgcgt cgtgaacgac gtcccgccga aggaccgcga catagcgatg gtgttccaga
     1141 actacgcgct gtacccgcac atgagcgtct acgagaacat ggcgttcggc ctcaagctgc
     1201 gccgggtctc gaaggacgag atcgaccgcc gggtccgcga ggcggcgcag atcctcggcc
     1261 tcggggagct gctctcgagg aagccgcgga acctctccgg cggccagcgg cagcgcgtcg
     1321 cgatggggcg ggcgatcgtt cgcgagccgc aggcgttcct catggacgag ccgctctcga
     1381 acctcgatgc caagctccgc gtgcagaccc gcgcggaggt cgcgcggatc cagcgagagc
     1441 tcgaggtcac gacgatctac gtgacgcacg accagaccga ggccatgacg ctcggcgacc
     1501 gcgtcgccgt gatccgcaag gggcgcctcc agcaggtcgc gcccccgcag acgctctacg
     1561 acaacccagt gaacctgttc gtcgcgggct tcatcggatc gccggcgatg aacatggtgg
     1621 aggcggagct cgtgcgcgac gacggcgggc tggcggtcga gttcggcggc gcccgcttcg
     1681 acgtcgacga cgaggcgctc gccgaccgtc cgcggctcaa ggagttcgag ggtcggaagc
     1741 ttatcctcgg gatccggccc gaagacatgg aggacgcaga ggtcgtcggc gacgccccga
     1801 gcgaccgacg gttctcgacg accgtcgagc tgcgcgagga cctcgggtcc gaggtgctcg
     1861 tccacttctc ggtcgacgag ccgatcgtgc tgaccgagga cacgaaggag ctcgccgccg
     1921 acgtcggcac cgaggcgctg gagtcgctcg aggacagcgc gcaacgggat cggtcgaagt
     1981 tcgtcgcgcg gctctcgccg acctcgaagg ctcgggaggg ccagcaggtc gagctgttca
     2041 tcgacacccg gcgcctccac ttcttcgatc ccgagtcggg gctcgggatc tccgacgggg
     2101 aggggaagga agcgacctag gaggagcgtg gccgcaggcg cccggacgat ccctgcccgc
     2161 tcttccggtc cgctcgcgtg ggtcgggcgg caccggttcg agctcgcgct gatcgcgccg
     2221 ctcgtcgtct acgtcacggt cctgacgatc gctccgatct tcgacacgat ccggatctcg
     2281 ctgctcggcg aggacggtgc cttcacgacc gacaactacg gccggatctt cgggcagcag
     2341 gtgttccgcc aggcggtcgt caacaccgtg atcgtcgcgc tcgtctcgct gacgctcgag
     2401 ctgacggtcg ggctcgcgat cgcgctcgcg ctcaacgcga agttcttcgg gcgcggcttc
     2461 gtgcggaccg tgacgctcat cccgctgggc gtcccgacga tcgtgtcggg agccgtgatg
     2521 ctgctgctgt tcggctcctc cggctacctc aactcgatct tcgggttcgt cgtcgacctc
     2581 ttcaacaaga tcccgggcgt cgagatgcag ttccgcggca tcgactggac gatcaccggg
     2641 ggcctccgca cgctgttcac gatcgcgatc gccgacatgt ggaaggtcct gccggtcatc
     2701 acgctgatct tcctcgcggg gctccagtcg atccccgagg acgtctacga ggcggcggac
     2761 gtcgacggcg ccacgaagtg gcagcggttc tcgcgcgtga cgctcccgct gctcatcccc
     2821 tacatcacga tcgcgctgat ccttcgcgcg atcgacgcgt tccggatctt cgagctggcc
     2881 ctggtgctgt cggggcggat cgagccggtg ctcgggacgt acatctggaa ccagtacgcg
     2941 ccgccgacga gcaacccgtt cacctcggcc gccgcgtcga tcgtgctgtt cgggatcatc
     3001 atggtgttca tcatcatcta cctgcgcgtc gtcgctcggc gcggggacgt ccagctgtga
     3061 gcgcgacgcc gggcaccgcc gtcgcgacgc ggaagacgca cacgggcccg cggcgcccgc
     3121 ggccgcggcg cgacgtgctc gacaagccgc tgaaggtcat cttcccgatc tggatcgtcg
     3181 tcacggtcct gatcgtgctg ttccctctct atctgatctt cctcgtgtcg ttcgcgccgg
     3241 ggatcgcgct gttcggcgag cggccggacc tgttcaccag cgagccgacg ctgcaatggt
     3301 ggcggagcgt ggtcgagggc ggcgacctca tcccgccgct catcaagagc ctcgtcgtcg
     3361 cgaccgtgac gacgctcgtc gcgatcgtca tcgcggcgcc cgccgcgtac gtgatctcgc
     3421 ggctgcgccc ggcggtgcgc tacacgatcg tgatcgggct gctcgtgacc cggatgttcc
     3481 cggagttcgc gatcggcatc tcggtcgcga cgcgcttcgc gaagctcggc ttcatcgacg
     3541 actacaccgg ggtgatcctg gcgcacctca tcggcgcgct cccgttcatc gcgtggatcc
     3601 tcgtcgggac gttcgagacg atcccccgcg agctggagga ggcgtcggcg atcgacggcg
     3661 cgtcgcggct cgggacgctg atacgggtcg tgatgccgct cgcgctgccc ggcatcgcgg
     3721 tcgcggcgct gttcgtgtgg ctctactcgt ggaacgagtt cgtgttcgcg cggctgctga
     3781 ccacgacgca gaacacgctc ccgcttcagg tgttccaggc gatcgaccgc gggagcaagc
     3841 agcagatggc gacggtcgcc gccgtgctga ctctgccgat cctgttcgtc gtgtacttcc
     3901 tccagcggta cctgcgcccc ggcgctctga ccggagccgt gaaagggtag agagaggggg
     3961 agtcgagggg gtccgtaccc cgaggaggtg ggtggtatga ggaagaggca tttgctggtc
     4021 gcgacggcgg cggctctgtc gctggtcgcg gcggcgtgcg ggggggcgcc cgagacgccc
     4081 ggaggacccg gagacgaagg cgacctctcc ggcgcagaga tcacgttcaa cgtctcgctc
     4141 gccgaggagg agcaggcggg ggtccaggcg gtcctcgacc agttcacgca gcagaccggc
     4201 gcgaacgtga agctgtcgtc ggtcaccgcg gaccagatgc cgcagaagct gcaggtcgag
     4261 gtcgaatccg gcaacaacac gatccatctg atctcgcagg acaactacat cctcgagccg
     4321 ctcgtcgcgg acgggctcgt ccaggacgtc ggcgacgtcg agatcccggc cgaggtctcc
     4381 gaggcgctga tcccggcgca gttcgacggc acgacgtact tcctgccgtt ccggccgaac
     4441 gtccgcgtcg cgtacgcgaa cacccagcgc ttcgaggagg ccggcgtgtc ggggccgccg
     4501 acgacccacg aggaatggcg ctcgatggcg gaggcgttcg cccagacggc cggcgagccc
     4561 aaggtgacga tctcgttcgc ggagggatcc tcgaccggcg tcgtcgcgtc ggagtggatc
     4621 ctgtcgttcg gcgggaaccc gctgatcctc aacgacgagg ggtcggtcgc cgcgttcgag
     4681 ttcctccagg acgcgtggaa cgacggg
//
LOCUS       DASZOB010000366         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98601, whole genome shotgun sequence.
ACCESSION   DASZOB010000366 DASZOB010000000
VERSION     DASZOB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98601"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VG709_05860"
     CDS             complement(<1..612)
                     /locus_tag="VG709_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWC32637.1"
                     /translation="MRAVVYEDVKQVRVDDVDDPKVEEPGDAVVRVTMSAICGSDLHV
                     YTGKAPLMPGDQIGHEGVGVVEEVGEGVSRVKPGDRVVIAFNIVCGECWFCENGQTSL
                     CDAFRNLGMGIIGGNLGGTQAERIRVPNADHNLLKLPDDVDDDRAVFLGDILTTGYYG
                     AAIAGIRPGDTVAIIGAGPVGFFAAQAARLHDPKDVRVVDMVPD"
     gene            complement(609..1037)
                     /locus_tag="VG709_05865"
     CDS             complement(609..1037)
                     /locus_tag="VG709_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWC32638.1"
                     /translation="MPEFPSEEWLKEFIDRVNASDAYRQAAAQWEGDVCFVFEAEPDK
                     GVPEDIYGILELWHGECRGGRLVPKDEGESAPFVIRAPYSRWKEVIKRELDPVKGMMQ
                     GKLKLKGDLPTIVRYVKASNELVNLATTVPTEFVDEKAGA"
     gene            1235..4234
                     /locus_tag="VG709_05870"
     CDS             1235..4234
                     /locus_tag="VG709_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWC32639.1"
                     /translation="MKPSKHSRLFVSLVSTLTASMVVVGPGVGPAAAAPPAGQFLNPD
                     EDGPGNVISDKPDGTDGRYHFVLRLRDPEGLDDIQSVEIQIEDGDANTTFETIGVANR
                     VQTTDNPPAGTDTWELRWDLSAHAPASDVNNDPGAVRAAVFTHAEGGPTFVPSAAGDP
                     VEFRDQAAETVEITSPPNGDALGFFDADGDGNRDALIQGTASSGITTDEEIELFYTTQ
                     ADPAVEPVWVDCEEGVVVRPNGWRGFCELSGGATPAQVTAVAVRTDDDNASGETGDES
                     GDAHRVFGYLQAANALDVVPAEDTETIGFCNTYTVSARDQRNRPILGAPIDVHATGPA
                     DDLQFAANGDDPDDPDETSDPFAAPLLLHTSEPAAGCDQQEASPDAPDDNDSNSTGQQ
                     GEHENLTGADTKHIEGRTDLNGFQFSLDSETAGATGIRAWFDADDDEAEDTGEPAVDA
                     AKTWVAAAAANVNAVPETDSNATGATHTVRATVTDENGNPVPGQRVHFRVTSGPHADD
                     NLDEFVSTPRGYFGSCETDANGRCSQSYTGDEAGTDTITVWVNAGQETAADPSGTSPA
                     FTPDADDRKDTVSKTWVPEPTVIDCGPENATNQAGANHSITCTVTDAFGNPVQGANVD
                     FRVVAGPHADDDLDANDGTPPGYIGESTTDQTGQASTFYGGKETRPNRGNDVIDAWID
                     RNDNDVDDGDAVEGDGGDADEIVEAEDQTDEGERSDYVDKTWTPGAPVVIVCSPETST
                     NVVREQHPVTCVVQDLFGNPTPGVNVDFRVVAGPHADDDLDGSTETPGGYFGEGGTDA
                     DGSVTRSYIGTETAPNGGNDVIDGWIDTNDSDTDDGDDVEGDGPDADQIVEAEDQTDE
                     GEPSDYVDKTWKEPPVEECPGYEGDPRNDVVGSQTRDRLVGTPGPDIMCGLARRDVLI
                     GRGGHDLILGGGGDDEIRGADGNDDLRGGRGNDEIAGGDGADTLRGQAGHDELYGNAG
                     RDRLIGGPGSDLCRGGRGRDSTRGCER"
     gene            complement(4288..5088)
                     /locus_tag="VG709_05875"
     CDS             complement(4288..5088)
                     /locus_tag="VG709_05875"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWC32640.1"
                     /translation="MTRATIDEAASVVTGGGVVAFTGAGISAESGIPTFRDPGGLWDR
                     FDPSDFGTWDGLARTAMERPDALAEFLAQLRRVFAGAQPGEAHRALARLGDAGIVDAV
                     ITQNVDGLHQDAGSSDVIELHGSFRRVGCLVAGHRSVVPKEEFLASLDRAIVGLRTAF
                     VPSLASLLPRCPVCDAPARPDFVAFGEGVHDFDVAERRAREARVVLVVGTSGEVFPAA
                     HIPESAKAAGAVVVEIAGGESFIQSDLRIEGQAGALLPALGDAALDMR"
BASE COUNT          908 a         1834 c         1836 g          614 t
ORIGIN      
        1 gtcggggacc atgtcgacca cgcggacgtc cttcgggtcg tgcagcctcg ccgcctgtgc
       61 ggcgaagaag ccgacggggc ccgcgccgat gatcgcgacg gtgtccccgg gccggatgcc
      121 ggcgatcgcg gccccgtagt acccggtcgt gaggatgtcg ccgaggaaca cggcgcggtc
      181 gtcgtcgacg tcgtcgggga gcttcaggag attgtgatcg gcgttcggga cgcggatccg
      241 ctcggcctgc gtcccgccga ggttcccgcc gatgatcccc atcccgaggt tgcggaaggc
      301 gtcgcacagc gacgtctggc cgttctcgca gaaccagcac tcgccgcaca cgatgttgaa
      361 cgcgatcacg acgcggtcgc cgggcttcac gcgcgagacg ccttctccga cctcctcgac
      421 gaccccgacg ccttcgtggc cgatctggtc gccgggcatg agcggcgcct tccccgtgta
      481 gacgtggaga tccgagccgc agatcgccga catcgtcacc cggacgacgg cgtcgccggg
      541 ctcctcgacc ttcgggtcgt cgacgtcgtc gacgcggacc tgcttgacgt cctcgtagac
      601 gacggccctc atgcgccggc cttctcgtcg acgaactccg tcggcaccgt cgtcgcgagg
      661 ttcacgagct cgttcgaggc cttgacgtac ctgacgatcg tcgggaggtc gcccttcagc
      721 ttcagcttgc cctgcatcat ccccttgacc gggtcgagct cgcgcttgat gacctctttc
      781 catcgcgagt acggcgcacg gatcacgaac ggcgcactct ccccctcgtc cttggggacc
      841 aggcgcccgc cgcggcactc gccgtgccac agctcgagga tcccgtagat gtcctcggga
      901 acacccttgt cgggctcggc ctcgaagacg aagcacacgt ccccttccca ctgcgccgcg
      961 gcctgccggt aggcgtcgct ggcgttgacg cggtcgatga actccttgag ccactcctcg
     1021 ctggggaact cgggcatccg acctcctcgg cttctgacgc ggggtcagta aagagcacgg
     1081 ctcgcccggg ggcaaccgcc ggagcgcact cgacgctccg gagcgtacaa gagaagaagg
     1141 agatcccggg cggaacgtcg aacgtggacg agcagggaaa gccggatcga agcggtgccg
     1201 ggcgacccgg cgccgtcggg aagggggtct atccgtgaag ccgagcaagc atagtcggct
     1261 gttcgtctcg ctcgtcagca cgctcaccgc ctcgatggtt gtcgtggggc ccggcgtggg
     1321 gccagccgcc gcagccccgc ctgcggggca gttcctgaac ccggacgagg acgggccggg
     1381 gaacgtcatc tcggacaagc ccgatggcac cgacgggcgc taccacttcg tgctgcggct
     1441 cagggacccc gagggtctcg acgacatcca gagcgtggag atccagatcg aggacgggga
     1501 cgcgaacacg acgttcgaga cgatcggcgt cgccaaccgg gtccagacga cggacaaccc
     1561 cccagccggg accgacacgt gggagctccg gtgggacctg agcgcgcacg ccccggcgag
     1621 cgacgtcaac aacgaccccg gggcggtgcg ggcggcggtg ttcacgcacg ccgagggcgg
     1681 ccccacgttc gttccgagcg cagcggggga ccccgtcgag ttccgcgacc aggcggccga
     1741 gacggtcgag atcacgagcc ccccgaatgg agacgcgctc gggttcttcg acgccgacgg
     1801 cgacgggaac cgcgacgcgc tcatccaggg aacggcctcg agcggcatca ccaccgacga
     1861 ggagatcgag ctcttctaca ccacgcaggc ggatccggcg gtggaacccg tgtgggtcga
     1921 ctgcgaggag ggcgtcgtcg tccggccgaa cgggtggcgg ggattctgtg agctgagcgg
     1981 tggcgcgacc cccgcacagg tcactgccgt ggcggtccga acggacgacg acaacgcctc
     2041 gggcgagacc ggggacgaga gcggcgacgc gcaccgggtc ttcgggtacc tccaggcggc
     2101 gaacgccctc gacgtcgtcc cggccgagga caccgagacc atcggcttct gcaacacgta
     2161 cacggtgagc gcgcgcgacc agaggaaccg cccgatcctc ggagcgccga tcgacgtcca
     2221 cgcgaccggg ccggccgacg atctgcagtt cgcggcgaac ggcgacgacc ccgacgatcc
     2281 cgacgagacc agcgacccct tcgcggcgcc gctgctgctc cacacgtcgg agcccgcggc
     2341 cgggtgcgac cagcaggagg cgtctcccga cgcgcccgat gacaacgact cgaactcgac
     2401 cgggcagcag ggagagcacg agaacctcac cggtgcggac accaagcaca tcgagggccg
     2461 cacggacctg aacgggttcc agttctcgct cgactcggag acggccggag ccaccggcat
     2521 ccgtgcttgg ttcgacgccg acgacgacga ggcggaagac accggcgagc ccgcggtcga
     2581 cgccgccaag acgtgggtgg ccgctgccgc ggcgaacgtc aacgccgtcc cggagaccga
     2641 ctccaacgcg accggcgcca cgcacaccgt gagggcgacg gtgaccgatg agaacggcaa
     2701 ccccgtgccc ggacaacggg tgcatttccg ggtgacgtcc ggaccgcacg cggacgacaa
     2761 cctcgacgag ttcgtgtcga cccctcgcgg ctacttcggg agctgcgaga ccgacgcaaa
     2821 cggccggtgc agccagagct acacgggcga cgaggccggg accgacacca tcaccgtctg
     2881 ggtcaacgcc ggccaggaga cggccgccga cccgtccgga acgagccccg cgttcacccc
     2941 ggacgccgac gatcgcaagg acacggtcag caagacgtgg gttccggagc cgaccgtcat
     3001 cgactgcggc cccgagaacg ccaccaacca ggccggggcc aaccactcca tcacgtgcac
     3061 ggtgaccgat gccttcggca accccgtgca aggcgcgaac gtcgacttcc gcgtcgtcgc
     3121 cggtccgcac gccgacgacg acctcgacgc gaacgacggg acgccgccgg gatacatcgg
     3181 cgagagcacc accgaccaga ccggccaggc ctcaacgttc tacggcggga aggagacgcg
     3241 gccgaaccgg ggcaacgacg tgatcgacgc gtggatcgac cggaacgaca acgacgtcga
     3301 cgacggcgac gccgtggagg gcgacggcgg cgatgccgat gagatcgtcg aagccgagga
     3361 tcagaccgac gagggagagc ggagcgacta cgtcgacaag acgtggacgc cgggagcgcc
     3421 ggtcgtgatc gtgtgcagcc cggagacctc gaccaacgtc gtgagagagc agcacccggt
     3481 gacctgcgtc gtgcaggatc tcttcgggaa cccgacgccc ggcgtgaacg tcgacttccg
     3541 ggtagtggcc gggccacacg ccgacgacga cctcgacggg agcacggaga cgcccggcgg
     3601 gtacttcggc gagggaggca ccgacgcgga cgggagcgtg acgcgctcct acatcgggac
     3661 agagaccgcc cccaacggcg ggaacgacgt catcgacggc tggatcgaca cgaacgacag
     3721 cgacaccgac gacggcgacg acgtggaggg cgacgggcca gacgccgacc agatcgtcga
     3781 ggccgaggac cagaccgacg agggagagcc gagcgactac gtcgacaaga cgtggaagga
     3841 accgcccgtc gaggaatgcc ctgggtacga gggcgacccg cggaacgacg tggtcggaag
     3901 ccagacgcgc gaccgcctcg tcgggacgcc gggcccggac atcatgtgcg gcctggcgcg
     3961 gcgtgacgtc ctgatcggcc gtggaggtca cgacctcatc ctcggaggcg gcggagatga
     4021 cgagatcagg ggcgcagacg gcaacgacga cctccgcggc ggcaggggca acgacgagat
     4081 cgcgggcggc gacggcgccg acacgctccg cggacaggcc ggccacgacg agctctacgg
     4141 gaacgcgggg cgggaccgcc tgatcggcgg gcccggtagc gacctgtgcc gtggcgggcg
     4201 cggccgcgac agcaccaggg gctgcgagcg gtagcgaggc gctgcggagg tgggcggcgg
     4261 tccggctccg tgccgggccg ccacccttca ccgcatgtcg agcgcggcgt cgcccagcgc
     4321 agggagcagc gcgccggcct gaccctcgat ccgcagatcg gactggatga aggactcgcc
     4381 gccggcgatc tcgacgacga cggccccggc ggccttcgcg ctctcgggga tgtgggccgc
     4441 cgggaacacc tcgccggagg tcccgactac gaggacgacg cgggcctccc gcgcgcgccg
     4501 ctccgcgacg tcgaagtcgt gcaccccctc cccgaacgcg acgaagtccg gacgagcggg
     4561 cgcgtcgcac acggggcacc gcggaaggag cgacgcgagg ctcgggacaa acgcggtccg
     4621 caggccgacg atcgcgcggt ccagcgacgc gaggaactcc tccttgggca cgacgctccg
     4681 gtgtccggcg acgaggcagc cgacgcggcg gaacgacccg tggagctcga tcacgtcgct
     4741 gctcccggcg tcctggtgga gcccgtcgac gttctgcgtg atcaccgcgt cgacgatccc
     4801 cgcgtcgccg agcctcgcga gcgcgcggtg cgcctcgccg ggctgggcgc ccgcgaagac
     4861 cctccggagc tgggcgagga actccgccag cgcgtcggga cgctccatcg cggtccgcgc
     4921 gagcccgtcc cacgtcccga agtccgacgg gtcgaacctg tcccagagcc cgccggggtc
     4981 ccggaacgtc gggatcccgg actccgcgga gatcccggcc cccgtgaacg cgacgacgcc
     5041 gcccccggtg acgacagaag ccgcctcgtc gatcgtcgcg cgcgtcacgt cgagaacaac
     5101 cgctcgtaga tgatcagcgt gtacccgatc gcattgaacg tcgcgtgcgc cgcgatcgac
     5161 gcgaccagag accgccgcca ctcgtacagg aa
//
LOCUS       DASZOB010000367         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_8132,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000367 DASZOB010000000
VERSION     DASZOB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8132"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1862
                     /locus_tag="VG709_05880"
     CDS             60..1862
                     /locus_tag="VG709_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32641.1"
                     /translation="MRIYQPGSHLTRGHGAEVDDWSWTRTARRVGFLAGLTRPYGRRT
                     VLAVASLLAAVATALAPPFLAKVAIDEGIRGRDLTLLGWVVAAFLGAALLNWGANAAQ
                     TYYTAWVGERVLTDLRQRLFAHLQRLSLGYYERNRAGAVISRLTNDVEALDLLVTEGM
                     TSLVQNTLTLGGSAVILLFLDWRLALATLAVFPLMSLATAIFRRRSSRAYRRVRERLG
                     MVTATLAEDIAGMRVVQSFTRERRNAERFREVNDHYRAANHETVVLNGLYFPFVDFLS
                     STATAVVLGYGGYLVFDGDMTIGTLFAFIGYLSNFFDPVQQLSQLYNTFLAATAALDK
                     VIDVMDEEPEVVDQPGAIALPQIDGRVAFDGVRFGYGSGPEVLHGIDLDVPPGTTVAL
                     VGHTGAGKSTIAKLLARFYDPRDGSITIDGHDLRDVTQESLRRQLGIVPQEGFLFAGT
                     VRENILFGRPEATPEEVAAAARAGGAHEFVLELENGYETEVAERGSRLSLGQRQLVAF
                     ARALLADPRILILDEATSSVDIGTERRIEAGLRRLLADRTAFIIAHRLSTIRRADLIV
                     VLEHGRVVEQGTHEELMAREGVYLSLYGDWAAAA"
BASE COUNT          266 a          755 c          668 g          307 t
ORIGIN      
        1 gcggcgcacg ggctcgtcca ggcgcgggtc ctcacggaaa cggagggggc gaccgcctag
       61 tgaggatcta ccagccggga tcgcacctga cgcggggcca cggcgccgag gtcgacgact
      121 ggagctggac gcgcacggcg cgccgcgtcg gcttcctcgc cggcctcacg cgcccctacg
      181 ggcgacggac ggtgctcgcc gtcgcctcgc tcctcgcggc ggtcgccacg gcgctcgctc
      241 cgcccttcct cgccaaggtc gcgatcgacg agggcatccg cgggcgcgac ctcacgctcc
      301 tcggctgggt cgtcgccgcg ttcctcggcg cggcgctcct caactggggc gcgaacgccg
      361 cgcagacgta ctacacagcg tgggtcggcg agcgggtcct caccgacctg cggcagcggc
      421 tcttcgcgca cctccagcgg ctctcgctcg gctactacga gcgaaaccgc gccggcgccg
      481 tcatctcccg cctcacgaac gatgtcgagg cgctcgacct gctcgtgacc gaggggatga
      541 cgagcctcgt ccagaacacg ctcacgctcg gcggatcggc cgtcatcctg ctctttctcg
      601 actggcgtct cgcgctcgcg acgctcgccg tcttcccgct catgagcctg gccacggcga
      661 tcttccggcg ccgttcctcg cgcgcgtacc ggcgcgtgcg cgagcggctc gggatggtga
      721 cggcgacgct cgcggaggac atcgccggca tgcgcgtcgt ccagtcgttc acgcgcgagc
      781 ggcggaacgc cgagcgcttc cgcgaggtca acgaccacta ccgcgccgcc aaccacgaga
      841 cggtcgtgct gaacggcctt tacttcccgt tcgtcgactt cctctcgtcc acggcgaccg
      901 cggtcgtcct cggctacggc ggctacctcg tcttcgacgg cgacatgacg atcgggacgc
      961 tcttcgcctt catcgggtac ctctcgaact tcttcgatcc cgtgcagcag ctctcgcagc
     1021 tctacaacac cttcctcgcc gcgaccgcgg ctctcgacaa ggtgatcgac gtcatggacg
     1081 aggagccgga ggtggtcgac cagccgggcg cgattgccct cccgcagatc gacggacgcg
     1141 tcgccttcga cggcgtccgc ttcggctacg gctcggggcc ggaggtgctg cacgggattg
     1201 acctcgacgt cccgcccggg acgacggtcg cgctcgtcgg ccacaccggc gccggaaagt
     1261 cgacgatcgc gaagctcctc gcccgcttct acgacccgcg cgacggctcg atcacgatcg
     1321 acgggcacga cctgcgcgac gtcacccagg agagcctgcg ccgccagctc gggatcgtcc
     1381 cgcaggaggg gttcctgttt gcgggaacgg tccgcgagaa catcctgttc ggccggccgg
     1441 aggcgacgcc cgaggaggtc gcggccgcgg cccgtgccgg cggcgcgcac gagttcgtcc
     1501 tcgagctcga gaacggctac gagaccgaag tcgccgagcg cggctcgcgg ctctcgctcg
     1561 ggcagcgcca gctcgtcgcc ttcgcgcgcg cgctcctcgc cgacccgcgc atcctcatcc
     1621 tcgacgaggc gacgtcgagc gtcgacatcg gcacggagcg gcggatcgag gcgggcctgc
     1681 gccgccttct cgccgatcgg acggcgttca tcatcgccca ccgcctctcg acgatccggc
     1741 gcgccgacct gatcgtcgtc ctcgagcacg ggcgcgtggt cgagcagggg acgcacgagg
     1801 agctgatggc gcgagagggg gtctacctgt cgctctacgg cgactgggcc gccgccgcgt
     1861 gacgtacgct ccggccgtga acgtcacgag cggcgtcatc ggcgaggcct gggggatgta
     1921 caaggcgcac tggcgccacc tcgtcccgat cgcgctcgcc gtctacctcc tgctcgcgct
     1981 cttcaccctc ttcctc
//
LOCUS       DASZOB010000368         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78355, whole genome shotgun sequence.
ACCESSION   DASZOB010000368 DASZOB010000000
VERSION     DASZOB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78355"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VG709_05885"
     CDS             <1..526
                     /locus_tag="VG709_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32642.1"
                     /translation="PSALATATTRQLTPGSDTYRFRTRAVDKAGNMSRFAVGPAFRIT
                     AFDDSHAAIVDKGTWTTVALTGAYGGAVQHSSAAGTTATLSVPPGTKNVAWVTTRGPD
                     RGRARVCIDPGPASASCTTVDLYASPTALRRVVFARPVSPAAAHKVEVTVLGQKNASS
                     TGTRVDVDAFLTTT"
     gene            608..1024
                     /locus_tag="VG709_05890"
     CDS             608..1024
                     /locus_tag="VG709_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32643.1"
                     /translation="MRRSSRRSSPRRAGTRRQRAGVKAVGYTQTDRVESAADRAGFEV
                     ASPMALALTTRRLLSFAVSNPIGMGIGLKVEGLLGSVPISAVDAIDVGWLLIGSRLTV
                     TVRGTPVKLEGGPGAPTRRFARDFADLEAGLRSSTA"
     gene            complement(1162..1491)
                     /locus_tag="VG709_05895"
     CDS             complement(1162..1491)
                     /locus_tag="VG709_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWC32644.1"
                     /translation="MAYDEGLAARIAEILSERVDVSERKMFGGIAFMVDGHICCGVIR
                     DDLLLRLGPSSAETALEQPHVRPMDFTGRSMKGFVCVGPKGVRTEQQLRKRLDSALEF
                     VAALPPK"
     gene            complement(1495..>2034)
                     /locus_tag="VG709_05900"
     CDS             complement(1495..>2034)
                     /locus_tag="VG709_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWC32645.1"
                     /translation="MQAPGGADEDTSGGFEHGGGQGPYFDEAAGRHVDEAMQRAGGLL
                     LVRRTYEIFAGYWPTATEDRETADVLNSLPKYVASTTLEEPLEWSNSHLLKGDVAAEV
                     AALKQQDGKDLLVFGSGGRVHTLMRHDLVDEYRLQVNPIVLGSGKRLFDADTRVPLSF
                     VDTKVSTTGVLLLTYARGR"
BASE COUNT          305 a          705 c          716 g          308 t
ORIGIN      
        1 gccttcggcg ctggcgaccg ccacgacccg gcagctcaca cccggttccg acacgtatcg
       61 cttccgcaca cgcgcggtcg acaaggccgg gaacatgagc aggttcgccg tcggcccggc
      121 gttccgcatc accgccttcg acgactcgca cgcggcgatc gtcgacaagg ggacgtggac
      181 gacggtcgcg ctcaccggcg cctacggcgg ggccgtgcag cactcgagcg ccgcggggac
      241 gacggcgacg ctgagcgtcc cccccgggac gaagaacgta gcctgggtca cgaccagggg
      301 ccccgaccgc ggtcgggccc gcgtgtgcat cgatccgggg ccggcctcgg cgagctgcac
      361 gacggtcgac ctctatgcct cgccgaccgc gctccggagg gtcgtgttcg cgcgccctgt
      421 cagcccggcg gccgctcaca aggtcgaggt gacggtcctc ggccagaaga acgcgtcgtc
      481 aaccggcacc cgcgtagacg tcgatgcgtt tctcacgacg acgtaggcgc ccggcgcacg
      541 ggaaggagcg agatgtcgcg cagcgagaga gtgatcaagg aggcggcccg tcaggtcctc
      601 gagcccggtg aggagatcgt cgcggcgctc gtcgccgcgg cgcgcgggca cacgcaggca
      661 acgggccggg gtcaaggcgg tcggctacac gcagacggat cgggtcgagt cggcggcgga
      721 ccgggccggc ttcgaggtcg cctcaccgat ggcactggcg ctcacgaccc gccgcctcct
      781 gtcgttcgcg gtttcgaacc ccatcgggat ggggatcggc ctgaaggtcg agggcctgct
      841 cgggtcggtg ccgatttccg ccgtcgacgc gatcgacgtc gggtggctcc tcatcgggtc
      901 acgattgacc gtgacggtcc gagggacgcc cgtgaagctc gaggggggac cgggcgcgcc
      961 gacgaggcgt ttcgcgcgcg acttcgccga cctcgaggcc ggcctgcggt cctccaccgc
     1021 gtgacgagcc gcgtctgcgc agcctaaacg gggccgctgt cgcgtccggt cgtgggcaat
     1081 gccgaggaga tttgggaacc gagatcgccg attccctgga aaccgccgcg gttcggatcg
     1141 acgaggatag atcgcggaga gctatttggg cgggagcgcc gcgacgaact cgagcgcaga
     1201 atcgaggcgc tttcgcagct gctgctcggt tcggacgcct ttcggcccga cgcacacgaa
     1261 ccccttcatc gaacggccgg tgaagtccat cggccgcacg tgtggctgct cgagcgcggt
     1321 ctccgcacta ctgggtccga gacgtaggag caggtcgtca cggatcacgc cgcagcagat
     1381 gtggccgtcg accatgaacg cgatgccgcc gaacatcttc cgttcggaga cgtcgacgcg
     1441 ctcagagagg atctccgcga tccgcgccgc caggccttcg tcgtacgcca tctgttagcg
     1501 cccgcgcgcg tacgttagga ggagcacacc cgtcgtcgag accttggtgt cgacgaagct
     1561 gagtggaacc cgcgtgtcgg cgtcgaagag gcgcttgcca cttccaagca cgatcgggtt
     1621 gacctggagc cggtactcgt cgacgaggtc gtgccgcatc agcgtgtgga cgcgcccgcc
     1681 gctcccgaac acgaggaggt ccttcccgtc ctgctgcttg agcgccgcca cctcggccgc
     1741 gacgtcgccc ttgagcaggt gcgagttgct ccactcgagc ggctcctcga gcgtcgtcga
     1801 agcgacgtac ttcggaaggc tgttcaggac gtcggccgtt tcccgatcct ccgtcgccgt
     1861 gggccagtac ccggcgaaga tctcgtaggt gcggcgcacc aggagcagcc cgcccgcgcg
     1921 ttgcatcgcc tcgtcgacgt gccggccggc ggcctcgtcg aagtagggtc cctgcccgcc
     1981 gccgtgctcg aagccgccgc tcgtgtcctc gtcggcgccg cccggagcct gcat
//
LOCUS       DASZOB010000369         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_118068, whole genome shotgun sequence.
ACCESSION   DASZOB010000369 DASZOB010000000
VERSION     DASZOB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118068"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VG709_05905"
     CDS             <1..378
                     /locus_tag="VG709_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HWC32646.1"
                     /translation="PTPPAATAPPGGQGNDGEGAQQGDGGGNGGQEDVSIQVLNATDE
                     SGLAEDTAEFIESSLGYSDVVFGDASTDQDTTTIHFASGFRQQARTLRNELYREAVLQ
                     GGTAGDAAVDITVVLGRDYVEAN"
     gene            complement(375..1193)
                     /locus_tag="VG709_05910"
     CDS             complement(375..1193)
                     /locus_tag="VG709_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32647.1"
                     /translation="MRRLLPLVIVLATACATDTVELSYGLEPGRRLEYELRLSADVER
                     TLSREAVEQRIEATFRARQQILEPLSGGGATARMELLPTSLVIDGEPVNPGPSQAFIV
                     ALGHDGRVVEIDAPDEDDGAALGPVSVERLLPRLRPVLPGAAVAPGQAWRSDTTFTDR
                     DGRFSLESESRLERLGTVADREAALVRTTYVSPVDRREAFANAVAELDGTDVGVQEAW
                     FALDGFLLRSRGDSLGTYDVTFRPPGEEAGLQPVEGSLVVELHTEMVLVEEAGA"
     gene            1230..2003
                     /gene="otsB"
                     /locus_tag="VG709_05915"
     CDS             1230..2003
                     /gene="otsB"
                     /locus_tag="VG709_05915"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWC32648.1"
                     /translation="MDTAIGAIRADPERAGIFLDFDGTLSEIVTVPDAARAARGVSEV
                     LPVLAARYRVVAIVSGRQAADVAARLEIEEASLVRCYGLYGLELFGAPALLDAPLVDR
                     VAEAMPDVERAASYVPGALVEHKGLHVAVHYRSAPDAEAARRVLLERLSRTAAEVGLR
                     LLEGKRVVELAPGAGPTKGDVVRSVASSEDLAAVLYAGDDVADLDAFATLDALRERGV
                     TTTKVAVRSAETPESVVAAADVVVDAPPGLVAFLGRRSA"
     gene            complement(2009..>2291)
                     /locus_tag="VG709_05920"
     CDS             complement(2009..>2291)
                     /locus_tag="VG709_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWC32649.1"
                     /translation="FDGMNLVAKEGPALNENDGVLLLSRNAGAFAELGGDALPVDPLD
                     VAGTADAIHDALSLDHGARATRARALRDAATAMTPAEWVGRQLEDLAQA"
BASE COUNT          314 a          831 c          833 g          313 t
ORIGIN      
        1 ccgacgccgc cggcggcgac cgccccgccg ggtggtcagg ggaacgacgg cgaaggcgcc
       61 cagcagggtg acggcggggg gaacggcggg caagaggacg tcagcatcca ggtcctgaac
      121 gcgaccgacg aaagcgggct cgccgaggac accgcggagt tcatcgagtc gtcgctcggc
      181 tacagcgacg tggtcttcgg ggacgcgagc accgatcagg acacgacgac gatccacttc
      241 gcgagcggct tccgccagca ggcgcggacc ctccgcaacg agctctaccg cgaagcggtg
      301 ctccagggcg gcacggccgg cgacgccgcg gtcgacatca ccgtggtgct cgggcgcgac
      361 tacgtcgagg cgaactaggc gccggcctcc tcgacgagca ccatctcggt gtgcagctcg
      421 acgacgagcg acccctcgac gggctggagc cccgcttcct cgccgggcgg acggaacgtc
      481 acatcgtagg tcccgagcga gtctcccctc gagcggagca ggaacccatc gagcgcgaac
      541 caggcttcct ggaccccgac gtccgtgccg tcgagctcgg ccaccgcgtt cgcgaacgcc
      601 tcgcggcgat ccacggggga cacgtacgtc gttcgcacca gcgccgcctc gcggtcggcc
      661 acggtcccga gccgctcgag cctcgactcg ctctcgagcg agaagcgacc gtcgcgatcg
      721 gtgaacgtcg tgtcggagcg ccacgcttgg cccggcgcga ccgccgcgcc aggcaggacc
      781 ggacggagcc gtggaagtag ccgctcgacg ctgacggggc cgagggctgc gccgtcgtcc
      841 tcgtcggggg cgtcgatctc gacgacgcgc ccgtcgtggc ccagcgcgac gatgaacgcc
      901 tggctcggac ccgggttcac gggctcgccg tcgatcacga ggctcgtcgg gagcagctcc
      961 atcctcgcgg tcgcgcctcc ccccgatagc ggctcgagga tctgctgtct cgcccggaag
     1021 gtggcctcga tgcgctgctc gaccgcctcg cgcgacagcg tgcgctcgac gtccgcgctg
     1081 agccgcagct cgtactcgag gcggcggccg ggctcgaggc cgtaggacag ctcgaccgtg
     1141 tcggtcgcgc acgccgtcgc aaggacgatc acgaggggaa ggaggcgccg cacggctcgg
     1201 cgagcgtagc acgggtacgc tgcatccccg tggacaccgc gatcggcgcg atccgagccg
     1261 accccgagcg cgccgggatc ttcctcgact tcgacgggac gttgtccgag atcgtcacgg
     1321 ttccggatgc cgcgcgggcg gcgcgcgggg tgtcggaggt gctaccggtg ctcgccgccc
     1381 ggtaccgcgt cgtggcgatc gtgtcgggga ggcaggccgc cgacgtcgcc gctcgcctcg
     1441 agatcgagga agcgtcgctc gtccgctgtt acgggctcta cggcctcgag ctgttcggcg
     1501 ccccagcgct gcttgacgcc cccctcgtcg accgtgtcgc ggaggcgatg ccggacgtcg
     1561 agcgggccgc ctcctacgtc cccggggcgc tcgtcgagca caagggcctc cacgtcgccg
     1621 tccattaccg ttccgcaccc gacgcggaag cagcacgccg cgtcctgctc gagcgcctgt
     1681 cccgcacggc tgcggaggtc gggctccggc tcctcgaggg caagcgcgtc gtcgagctcg
     1741 cgcccggcgc cgggccgacg aagggagacg tggtccgctc ggtcgcctcg agcgaagacc
     1801 tggccgcggt gctctacgcg ggcgacgacg tcgccgatct cgacgcattc gcgacgctcg
     1861 acgcgctccg cgagcgaggc gtgacgacga cgaaggtggc ggtccgctct gctgagaccc
     1921 cggaatcggt ggtggctgcg gccgacgtcg tcgtcgacgc gcctccaggg ctcgttgcct
     1981 tcctggggcg ccgctccgcc tgaggcgttc aggcctgggc caggtcctcg agctggcgac
     2041 ccacccactc cgccggcgtc atcgcggtcg ccgcgtcgcg gagcgcacgt gctcgagtgg
     2101 cacgtgcgcc gtggtccagt gagagcgcgt cgtggatcgc gtcggcggtc ccggcgacgt
     2161 cgagcgggtc taccgggagg gcgtcgccac cgagctccgc gaaggctccg gcgttgcgtg
     2221 agagaaggag cacgccgtcg ttctcgttca gcgccgggcc ctccttcgcg acgaggttca
     2281 tcccgtcgaa c
//
LOCUS       DASZOB010000370         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98635, whole genome shotgun sequence.
ACCESSION   DASZOB010000370 DASZOB010000000
VERSION     DASZOB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98635"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="guaA"
                     /locus_tag="VG709_05925"
     CDS             complement(<1..1137)
                     /gene="guaA"
                     /locus_tag="VG709_05925"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWC32650.1"
                     /translation="MSDDPRPVLVVDFGAQYAQLIARRVRECHVYSEIVPHATPVAEL
                     LARRPAGLILSGGPASVYEPGAPSLDAEVFRLGVPVLVICYGQQLMAQSLGGEVAATG
                     VREYGKTDLRVTTPGTLFGDLPPRQTVWMSHADAVVRPPVGFRTTAATEATPVAAMED
                     PERGLFAVQFHPEVAHTPRGTEVMKRFLYEACGLLPDWTPITVVERAVEQVRRQVGDE
                     QVLCALSGGVDSSVAALLVHRAVGDRLSCVFVDTGLLRGGEPEQVEETFRGHFGVDLV
                     HVKAEDRFLARLAGVEDPEEKRRRIGEEFIRVFEEVARERTRARFLVQGTLYPDVIES
                     GTRDAARERAQGLLVADLVADAGGGALDHVHRRPVGQEVAGLVQE"
     gene            complement(1151..>1653)
                     /locus_tag="VG709_05930"
     CDS             complement(1151..>1653)
                     /locus_tag="VG709_05930"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWC32651.1"
                     /translation="ACTTRGVIGVGVPQATAIAAAAAARMEHLRETGRYCHVIADGGM
                     RTGGDVAKAIACGADAVMLGSPLARAYEAPGRGYYWGMATFHPTLPRGARVATKQIGT
                     LREILVGPAHENDGTLNLFGALRMSMATCGYANIREFQKAEVVIAPSIKTEGKDLQRA
                     QQVGMG"
BASE COUNT          225 a          616 c          596 g          216 t
ORIGIN      
        1 ttcctgtacg aggcctgcga cctcctgccc gactggacgc cggtgaacgt gatcgagcgc
       61 gccacctccc gcgtccgcga ccaggtcggc gacgaggagg ccctgtgcgc gctctcgggc
      121 ggcgtcacgg gtgcccgact cgatcacgtc cgggtacagc gtgccctgga cgaggaagcg
      181 cgctcgcgtc cgctcccggg cgacctcctc gaatacccgg atgaactcct cgccgatccg
      241 ccggcgcttc tcctcggggt cctcgacgcc ggcgagccgg gcgaggaagc ggtcctcggc
      301 cttgacgtga acgaggtcca cgccgaagtg tccgcggaag gtctcctcga cctgctccgg
      361 ctcgccgccc cgcagcagcc ccgtgtcgac gaagacgcac gagagccggt cgccgaccgc
      421 gcggtggacg agcagcgccg cgaccgagga gtcgacaccg ccggagagcg cgcagagcac
      481 ctgctcgtcc ccgacctgcc gccgcacctg ctccacggcc cgctcgacga ccgtgatcgg
      541 ggtccagtcg ggcaggagac cgcacgcctc gtagaggaag cgcttcatga cctcggtccc
      601 gcgcggcgtg tgcgcgacct ccgggtggaa ctgcacggcg aacaggccgc gctcggggtc
      661 ctccatcgcc gcgaccgggg tcgcctccgt cgcggcggtc gtgcggaacc ccacgggggg
      721 ccggacgacg gcgtcggcgt ggctcatcca gaccgtctgc cggggcggga ggtcgccgaa
      781 cagcgtgccg ggtgtcgtga cgcgcaggtc ggtcttgccg tactcgcgaa cgccggtcgc
      841 ggccacctcg cccccgagcg actgcgccat cagctgctgg ccgtaacaga tgacgaggac
      901 ggggacgccg agacggaaca cctccgcatc gagactcggg gcaccgggct cgtagaccga
      961 ggccgggccc cccgagagga tcaggcccgc cgggcggcga gcgagcagct ccgccaccgg
     1021 ggtggcgtgc ggcacgatct cggagtacac gtggcactcg cggacgcggc gggcgatcag
     1081 ctgggcgtac tgagcgccga agtcgacgac gaggacggga cgggggtcgt cgctcacggc
     1141 gcggaggggc ctatcccatc ccgacctgct gcgcccgctg gaggtccttg ccctcggtct
     1201 tgatcgaggg cgcgatcacg acctccgcct tctggaactc gcggatgttc gcgtagccgc
     1261 aggtcgccat cgacatccgc agcgcgccga agaggttcag tgtgccgtcg ttctcgtgcg
     1321 ccggcccgac gaggatctcc cggagcgtcc cgatctgctt ggtcgcgacg cgcgcgccgc
     1381 gcggcagcgt cgggtggaac gtcgccatgc cccagtagta gccgcggccg ggggcctcgt
     1441 aggcacgcgc cagcggcgag ccgagcatca cggcgtcggc cccgcacgcg atcgccttcg
     1501 cgacgtcgcc gccggtgcgc atcccgccgt cggcgatgac gtggcagtac ctccccgtct
     1561 cgcgcaggtg ctccatccgc gccgccgccg cggcggcgat cgcggtcgcc tgcggcacgc
     1621 cgacgccgat caccccgcgg gtcgtgcacg cgg
//
LOCUS       DASZOB010000371         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_186348, whole genome shotgun sequence.
ACCESSION   DASZOB010000371 DASZOB010000000
VERSION     DASZOB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186348"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VG709_05935"
     CDS             <1..979
                     /locus_tag="VG709_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWC32652.1"
                     /translation="SSGVAAARKVIERARGSMLQPVLFVDEVHRWSKAQQDVLLPAVE
                     EGTVTLIGATTENPYFSLVSPLLSRSLLLRLEPLAKEDVRAVLDRALADADRGVAGQG
                     IEVDDEALEYLAETGAGDARIALAGLDAAVQAAKGEGRKAVDRGFAEDVMQRPAVVYD
                     RDTHYDVISAFIKSLRGSDADAGLYWLARMLEAGEDPRFIARRMIILASEDVGMADPQ
                     ALPVAVAAAHAVEHVGLPEAQLNLAHAAVYLARAPKSNAVYTAIGRARADAAANAPVP
                     LHLRDAHYPGARKLGHGEGYVYPHDDPDGAKDQRYLPERVRGKRYYEPD"
     gene            1030..1386
                     /locus_tag="VG709_05940"
     CDS             1030..1386
                     /locus_tag="VG709_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWC32653.1"
                     /translation="MTAGEIALIVLAAFWALLVVALVFVSTRLFRVLDTTSRLLDEVR
                     EQTVPLLGDVRTTVQSVNKELERTDVILSSAGNITRRAERVTGLVDEFVSQPLIKAIS
                     FAAGVNRGIKRFRGDL"
     gene            1383..1607
                     /locus_tag="VG709_05945"
     CDS             1383..1607
                     /locus_tag="VG709_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32654.1"
                     /translation="MKRVFWFGVGVTAAVLAARWVRKQRARLAPANVGEQAGRAVRDL
                     RTLVSESVAEGRRAAREKEAELRGSYDAPR"
     gene            1617..>2078
                     /locus_tag="VG709_05950"
     CDS             1617..>2078
                     /locus_tag="VG709_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32655.1"
                     /translation="MPASAIAVRGLRKSYADVEAVAGIDLSVSEGEVFALLGPNGAGK
                     TTTVEILEGFRPRDAGEVLVLGSDPGRRDPELKRRIGIVLQETSVEPYMTVAEAIEMY
                     RGYYPAPRSLDEVLRLVGLKEQGDMKVRKLSGGQQRRLDVALGLAGDPELLF"
BASE COUNT          290 a          734 c          761 g          293 t
ORIGIN      
        1 gtcgtcggga gtcgcggccg cccggaaggt gatcgagcgg gcccgcggct cgatgctcca
       61 gccggtgctg ttcgtcgacg aggtccaccg ctggagcaag gcgcagcagg acgttctgct
      121 gccggcggtc gaggagggca ccgtgacgct catcggcgcg acgaccgaga acccgtactt
      181 ctcgctggtc tcgccgctgc tctcccgctc gctgctgctg cggctcgagc cgctcgcgaa
      241 ggaggacgtc cgggcggtcc tcgatcgcgc gctggccgac gccgaccgcg gggtcgctgg
      301 gcaggggatc gaggtcgacg acgaggcgct cgagtacctc gcggagacgg gcgccggcga
      361 cgcgcgcatc gcgctcgcgg gtctcgatgc tgcggtccag gcggcgaagg gcgagggccg
      421 caaggcggtc gaccgcggct tcgccgagga cgtcatgcag cgccctgccg tcgtgtacga
      481 ccgcgacacc cactacgacg tcatctcggc gttcatcaag agcctccgcg ggtccgacgc
      541 ggacgcgggc ctctactggc tcgctcggat gctggaggcc ggcgaggacc cgaggttcat
      601 cgcgcgccgc atgatcatcc tcgcgtcgga ggacgtcggg atggccgatc cgcaagcgct
      661 tccggtcgcg gtcgcggcgg cgcacgccgt cgagcacgtc gggctccccg aggcacagct
      721 caacctcgcc cacgcggccg tctacctggc gcgtgccccg aagagcaacg ccgtctacac
      781 ggcgatcggc cgggcccggg ccgacgccgc cgcgaacgcc cccgtgccgc tccacctgcg
      841 cgacgcgcac tatccgggcg cgaggaagct cggccacggc gagggctacg tctaccccca
      901 cgacgatccc gacggcgcga aggatcagcg ctatctcccc gagcgggtgc gcggcaagcg
      961 gtactacgag cccgactgag acccgcgagg ggctccgtta tgattggccg ccgtgacccg
     1021 gagcgacgca tgaccgcggg cgagatcgcg ctgatcgtcc tcgccgcctt ctgggcgctg
     1081 ctcgtcgtgg cgctcgtgtt cgtctcgacg aggctgttcc gcgttctcga cactacgagc
     1141 cgcctcctgg acgaggtccg cgagcagacc gtcccgcttc tcggcgacgt gcggacgacc
     1201 gtccagtcgg tgaacaagga gctggagcgg accgacgtca tcctgtcctc ggccgggaac
     1261 atcacccgcc gcgccgagcg cgtcaccggc ctcgtcgacg agttcgtctc gcagccgctc
     1321 atcaaggcga tctcgttcgc cgccggcgtc aaccgcggga tcaagcggtt caggggcgac
     1381 ctgtgaagcg ggtgttctgg ttcggcgtgg gcgtcacggc agcggtgctc gccgcacgct
     1441 gggtccggaa gcagcgcgcg cgcctcgcgc cggcgaacgt gggggagcag gcgggcaggg
     1501 cggttcgcga cctccgcacg ctggtctccg aatcggtcgc ggaggggcgt cgcgccgccc
     1561 gcgagaagga agcggagctc cgcggctcct acgacgcccc gcgctgaacc gcggggatgc
     1621 cggccagcgc gatcgcggtt cgcgggctcc gcaagtccta cgccgacgtc gaggcggtag
     1681 cggggatcga tctctcggtg tccgagggcg aggtcttcgc gctcctcggc ccgaacggcg
     1741 ccggcaagac gacgaccgtc gagatcctcg agggcttccg cccgcgcgac gccggcgagg
     1801 tgctcgtgct cggctccgac ccgggacgcc gagatccgga gctgaagcgg cggatcggca
     1861 tcgtcctcca ggagaccagc gtcgagccgt acatgacggt cgccgaggcg atcgagatgt
     1921 accgtggcta ctaccctgct ccgaggtcgc tggacgaggt gcttcgcctc gtcgggctca
     1981 aggagcaggg cgacatgaag gtccgaaagc tgtccggcgg ccagcagcgg cggctcgacg
     2041 tggcgctggg gctcgccggc gaccccgagc tgctgttc
//
LOCUS       DASZOB010000372         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_98728, whole genome shotgun sequence.
ACCESSION   DASZOB010000372 DASZOB010000000
VERSION     DASZOB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98728"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VG709_05955"
     CDS             complement(<1..798)
                     /locus_tag="VG709_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HWC32656.1"
                     /translation="MPARARPRARGEPGIAAKPRIALIGTLDTKGAEIAYVRDRLRDF
                     GADPLVIDSGILGEAAGCIPDYGRADVAREAGLDLDEIRNAGSRGAAVELMEQGVRAL
                     VLRLWLEGRLDGALCLGGAEGALLGAGALQALPVGVPKVIVSPSASGRRAFAPFVGES
                     DVLVMHSVVDILGLNTIARTVFDNAASAVAGMARTAGRPVQDLGQRTVGITMLGHPTP
                     AVMRIRAALEEADHEPVVFHANGVGGPAMEKLAATGALAGVIDYTLSE"
     gene            complement(753..>1536)
                     /locus_tag="VG709_05960"
     CDS             complement(753..>1536)
                     /locus_tag="VG709_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWC32657.1"
                     /translation="VRSLLDEHSLRLWGAVTLMVGGRDLLHEDKYVRIGSVQYVKDTI
                     DMIKALGGRILTVVPSTVGKVTPMGSPEDEWQWAVESLKECQAHAEKQGVRLALEPLN
                     RFETYFLNRCDQALALAEDVGGDCGVCLDIFHMNIEEADWRGAIRAAGDKLVDFHVAD
                     NNRMPPGQGAIDWELVVRTLDEVGYDAYLTVEFVATVDRSRISRRREIGDASEAEASI
                     GLEQFLRDHGTGAVPESLYDEYVHESIECLREHVRALEGSRV"
BASE COUNT          216 a          539 c          538 g          243 t
ORIGIN      
        1 ttccgagagc gtgtagtcga tcacgcccgc gagcgctccg gtggcggcca gcttctccat
       61 cgccggaccg ccgacgccgt tcgcgtgaaa gacgacgggc tcgtggtccg cctcctcgag
      121 cgccgcccga atgcgcatga ccgcgggggt cgggtgtccg agcatcgtga tgccgacggt
      181 ccgctgtccg aggtcctgca cgggtcgtcc cgccgtgcgc gccattccgg ccacagcgga
      241 cgcggcgttg tcgaagacgg tgcgcgcgat cgtgttcagg ccgaggatgt cgacgaccga
      301 gtgcatgacg aggacgtcgc tctcgccgac gaagggagcg aaggcccggc gccccgaggc
      361 gctcggcgag acgatcacct tcgggacgcc gacggggagc gcctgcagtg cgccggcgcc
      421 gagcagcgcg ccctccgcgc cgcccaggca gagcgccccg tcgagccgcc cctcgagcca
      481 gagccgcagg acgagtgcgc gcacaccctg ctccatcagc tcgacggccg ctccgcgact
      541 gcccgcgttg cggatctcgt cgaggtcgag gcccgcctcg cgcgcgacgt cggcgcggcc
      601 gtagtccggg atgcagccgg ccgcctcgcc gaggatgccc gagtcgatga cgagcggatc
      661 ggcgccgaag tcgcgcaggc ggtcgcgaac gtaggcgatc tcggccccct tcgtgtcgag
      721 cgtcccgatg agcgcgatgc ggggctttgc cgctataccc ggctcccctc gagcgcgcgg
      781 acgtgctcgc gcaggcattc gatcgactcg tgcacgtact cgtcgtacag gctttcgggc
      841 actgcgccgg tgccgtggtc gcgcaggaac tgctcgaggc cgatgctcgc ctcggcctcg
      901 gacgcgtcgc cgatctccct gcgtcgcgag atgcggctgc gatcgaccgt ggcgacgaac
      961 tcgaccgtga ggtaggcgtc gtagccgacc tcgtcgaggg tgcgaacgac cagctcccag
     1021 tcgattgccc cttggccggg cggcatgcgg ttgttatcgg ctacgtggaa gtcgacgagc
     1081 ttgtctcccg ccgctcgaat ggcgccccgc cagtccgcct cctcgatgtt catgtggaag
     1141 atgtcgaggc agacgccgca gtcgccgccg acgtcctcgg cgagcgcgag cgcctggtca
     1201 cagcggttca ggaagtaggt ctcgaagcga ttgaggggct cgagcgcgag gcggacgccc
     1261 tgcttctcgg catgcgcctg gcactccttc aggctctcga cggcccactg ccactcgtct
     1321 tccggcgatc ccatcggcgt caccttgccg accgtggagg ggacgacggt gaggatcctc
     1381 ccgccgagcg ccttgatcat gtcgatcgtg tccttgacgt actggacgct gccgatgcgg
     1441 acgtacttgt cctcgtgcag gaggtcgcgg ccgccgacca tcagggtgac cgctccccag
     1501 agcctgaggc tgtgctcgtc gaggagagag cgaacc
//
LOCUS       DASZOB010000373         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128752, whole genome shotgun sequence.
ACCESSION   DASZOB010000373 DASZOB010000000
VERSION     DASZOB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128752"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..852
                     /locus_tag="VG709_05965"
     CDS             43..852
                     /locus_tag="VG709_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC32658.1"
                     /translation="MRLVLRSKTFLAGALVVGFWVFCALFGEAVAPKSPFASDTLTSL
                     RAPSAEHWFGTDRLGRDVFSRVLTGARDILIVAPLATLLGTVLGTTLGLVTGYFRGLV
                     DDILMRFVDAVLAVPLVIMGLLVLVALGPSKVTVVMTIGIVFTPLIARTVRAAVLAEG
                     QLEYVQAARLRNERAPYIMFAEILPNVLAPVLVEFTVRLGYAIFVIATLSFLGLGIPP
                     PAPDWGLQIFEHYALISGGFWWPVLFPSLAIATLVVGVNLLADGLTQAFER"
     gene            855..>1749
                     /locus_tag="VG709_05970"
     CDS             855..>1749
                     /locus_tag="VG709_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32659.1"
                     /translation="MRPETQEPALSLRELEVSYRVRGRWREVLRNVSFEIARGESYGL
                     VGESGCGKSTAAFAVMRYLPRNGRVTNGSIHVGGRDVLAMSEREICRMRASQLSMVYQ
                     DPGAALNPSIRVGDQVAEVFTIAGVAKEEARERSREILAKVQISDPESVMRRYPHQLS
                     GGMQQRAVIAMALASDPTLLILDEPTTGLDATVEAEVLDLVLALREEFGTSVLFISHN
                     LAVIAKMCERVGVVYAGKLVEEGPADEIFADPRHPYTVGLLRCIPRRGVRKDEKRLDT
                     IPGSLPPIGAHLPACVFVDRCG"
BASE COUNT          236 a          647 c          569 g          297 t
ORIGIN      
        1 gctcttgggg gcgacgaagc ccgcaccgct cgacaggaac gcatccgtct cgtcctcaga
       61 tcgaagacgt tccttgccgg cgcgctcgtg gtcggcttct gggtcttctg cgcgctcttc
      121 ggggaggccg tcgcccccaa gagccccttc gcgagcgaca cgctgacgag cctgcgcgcg
      181 ccgtcggccg agcactggtt cggcacggac cgcctcggcc gcgacgtctt ctcgcgcgtc
      241 ctcacgggtg cgcgcgacat cctgatcgtc gcgcccctcg cgacgctgct cggaaccgtg
      301 ctcggaacga cgctcgggct cgtcaccggg tacttccgcg ggctcgtcga cgacatcctc
      361 atgcgcttcg tcgacgcggt tctcgccgtt cccctagtga tcatgggact gctcgtcctc
      421 gtcgcgctcg gcccttcgaa ggtgaccgtc gtcatgacga tcggcatcgt gttcacaccc
      481 ctgatcgcgc gcaccgtgcg cgcagccgtc ctcgccgagg gccaactcga gtacgtacag
      541 gcggcgcgcc tccgcaacga gcgcgcgccg tacatcatgt tcgccgagat ccttccgaac
      601 gtgctcgcac ccgtcctcgt cgagttcacc gtccggctcg gctacgccat cttcgtgatc
      661 gcgacgctct ccttcctcgg gctcggcatc ccaccgcccg cacccgactg ggggctccag
      721 atcttcgagc actacgcgct catctcgggc gggttctggt ggcccgtgct tttcccctcg
      781 ctcgcgatcg ccaccctcgt cgtcggcgtg aacctcctcg ccgacggcct gacgcaggcc
      841 ttcgagcgat agccatgcgg ccggagaccc aagagcctgc cctttcgctc cgcgagctcg
      901 aggtctccta ccgtgtgcgc gggaggtggc gcgaggtgct ccggaacgtc tccttcgaga
      961 tcgcccgcgg cgagtcgtac ggcctcgtcg gggagtccgg ctgcgggaag tcgaccgccg
     1021 ccttcgccgt catgcgctac ctgccgcgaa acggccgcgt gacgaacggc tccattcacg
     1081 tcggcggccg cgacgtcctc gcgatgagcg aacgcgagat ctgccggatg cgcgcgagcc
     1141 agctgtcgat ggtgtaccag gaccccggcg cggcgctcaa cccgtcgatc agggtgggcg
     1201 accaggtcgc ggaggtcttc acgatcgcgg gcgtggccaa ggaggaggcg cgcgagcggt
     1261 cgcgcgagat cctggcgaag gtgcagatct ccgatcccga gagcgtcatg cgccgctacc
     1321 cgcaccagct ctcgggcggg atgcagcagc gcgcggtgat cgcgatggcg ctcgcctccg
     1381 acccgacgct cctcatcctc gacgagccga cgacgggcct cgacgcgacc gtcgaggccg
     1441 aggtcctcga cctcgttctc gcgctccggg aggagttcgg gacgagcgtc ctcttcatca
     1501 gccacaacct cgccgtgatc gcgaagatgt gcgagcgcgt cggcgtcgtc tatgccggca
     1561 agctcgtgga ggaagggccc gcggacgaga tcttcgccga tcccaggcac ccgtacacgg
     1621 tcggcctcct ccgctgcatt ccgcgccgcg gtgtcaggaa ggacgagaag cggctcgaca
     1681 cgatcccggg gtcgctcccg ccgatcggcg cgcacctccc ggcgtgcgtc ttcgtcgacc
     1741 gctgcgggc
//
LOCUS       DASZOB010000374         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_175434, whole genome shotgun sequence.
ACCESSION   DASZOB010000374 DASZOB010000000
VERSION     DASZOB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175434"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VG709_05975"
     CDS             complement(<1..743)
                     /locus_tag="VG709_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32660.1"
                     /translation="MTALASLALAAALVPSAGAAEPRALYRASAVGTAIHGFYDHEGL
                     FPVPILNVSVPYTEATYEPGPTTSALGSFLWNPQAAELGTIVCVLSEGRFCEVPEYPF
                     QARAAYPAAGSSQPPPTVSVDEPGAPLRVRAAHERADADARGAGSEAEVAGFAAVPMT
                     DEQLDAARGLALAAAPFARQAPEMDRWLIAIGDATSRSSTRTANDGAVAESRAVLHDV
                     TLLGGVVRIDVVRGKALAVAPRSARARAES"
     gene            complement(749..1435)
                     /locus_tag="VG709_05980"
     CDS             complement(749..1435)
                     /locus_tag="VG709_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32661.1"
                     /translation="MLAGRDLVASYAGIRALHGASVEIPAGHVTAIVGPNGAGKSTLL
                     RVLSGLHRPDAGRVVLDGIDVTGLPAHGIARLGLAIVPEGRGTFPALTVEENVRIGAS
                     RRARARVLVAFPALRERRRQRAGALSGGEQQMLALGRAVATDAKVVLLDEPSLALAPR
                     LVDEIFRAVASLRAEGRTVVLVEQYVRRALELADLVYVMQRGRSVFVGEPAELSGRGA
                     LERAYLGVSR"
     gene            complement(1419..>2026)
                     /locus_tag="VG709_05985"
     CDS             complement(1419..>2026)
                     /locus_tag="VG709_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC32662.1"
                     /translation="RVVISGRDVTDWSPERRSALGLARTFQRLELFRRMTVFDNLLVA
                     AEAGFGEAEFVRDLFGRTRRTRAEALAREVLERVGLEALADRWADEVPTGIGRLVEIG
                     RALCTGPSALLLDEPAGGLDEAESDRLADVLRDLAAGEGLSLLLVEHDVRLVLDLCDR
                     IYVMDFGRMIAEGTPRQIRRDRAVHEAYLGEVPVGVDARRA"
BASE COUNT          294 a          779 c          712 g          241 t
ORIGIN      
        1 gactccgcgc gggcgcgcgc ggagcgcggg gcgacggcga gggccttccc tcgcacgacg
       61 tcgatccgga ccacgcctcc gagcagtgtc acgtcatgga ggaccgcacg actctcggca
      121 acggccccat cgtttgccgt cctcgtagat gagcgggacg tcgcgtcgcc gatcgcgatc
      181 agccaccgat ccatctcggg ggcttgccgc gcgaacggcg ccgcggcgag cgccaagccc
      241 cgagcggcgt cgagctgctc gtcggtcatc ggcaccgccg cgaaaccggc gacctcggcc
      301 tccgatcctg ctccacgcgc gtccgcgtcg gcgcgctcgt gtgcggctcg cacgcgcagc
      361 ggcgccccgg gctcgtcgac cgagaccgtc ggcggcggct gggagctccc ggctgccggg
      421 tatgccgcgc gggcctggaa gggatactcg ggcacctcgc aaaaccggcc ttcggagagg
      481 acgcacacga tcgtgccgag ctccgccgcc tgcgggttcc acaggaacga tccgagcgcg
      541 ctcgtggtgg gccccggctc gtacgtcgct tcggtgtagg gcaccgacac gttgaggatc
      601 gggacaggga agaggccctc gtggtcgtag aagccgtgga tcgcggtgcc gaccgccgac
      661 gctcggtaca gcgcgcgcgg ctccgcggcg ccggccgagg ggacgagcgc ggcggcgagc
      721 gccagcgagg cgagcgccgt cacccgtttc accgcgatac cccgaggtac gcgcgctcga
      781 gcgcgccgcg gccagaaagc tccgccggtt ccccgacgaa cacggatctc cccctctgca
      841 tcacgtagac gaggtcggcg agctccagcg cacgccgcac gtactgctcc acgagcacga
      901 ccgtacgacc ttcggcccgg agcgacgcga ccgcgcggaa gatctcgtcc acgaggcgcg
      961 gcgccagcgc gagcgacggc tcgtcgagca gcacgacttt cgcatcggtc gcgacggccc
     1021 ggccgagcgc gagcatctgc tgctcgccgc cggagagcgc gcccgcacgc tgacggcgcc
     1081 gctcccgcag cgcggggaac gcgacgagga cgcgcgccct cgcgcgtcgc gacgcgccga
     1141 tccggacgtt ctcctcgacc gtcagggccg ggaacgtccc gcgcccctcg gggacgatcg
     1201 ccaggccgag cctcgcgatg ccgtgtgccg gaagcccggt cacgtcgatc ccgtcgagca
     1261 ccacgcgccc cgcgtcgggg cggtggagcc ccgacagcac gcgaagaagc gtgctcttgc
     1321 cggcgccgtt gggcccgacg atcgcggtga cgtgacctgc ggggatctcc acggacgcgc
     1381 cgtgcagcgc ccggatccct gcgtaggacg cgacgaggtc acgcccggcg agcatcgacg
     1441 ccaaccggca cctcccccag gtaggcctcg tggacggctc gatcgcgcct gatctggcgc
     1501 ggcgttccct cggcgatcat ccgcccgaag tccatcacgt agatgcggtc gcacaggtcg
     1561 aggacgagcc gcacgtcgtg ctcgacgagc agcagcgata gcccctcgcc ggccgcgagg
     1621 tcgcggagca cgtcggcgag ccggtcgctc tccgcctcgt cgaggccgcc cgccggctcg
     1681 tcgaggagca acgcgcttgg gcccgtgcaa agcgcgcgcc cgatctcgac gagccggccg
     1741 atcccggtcg gcacctcgtc cgcccaccgg tcggcgagcg cctcgaggcc gacccgctcg
     1801 agcacctcgc gcgcgagcgc ctcggcgcgc gtacgacggg tccggccgaa gagatcgcgg
     1861 acgaactcgg cctcgccgaa ccccgcctcc gccgcgacga ggaggttgtc gaacaccgtc
     1921 atcctccgga acagctcgag gcgctggaac gttcgcgcca gcccgagcgc cgaccgccgt
     1981 tccggcgacc agtcggtcac gtcacgtccc gagatcacga cccgcc
//
LOCUS       DASZOB010000375         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_128803, whole genome shotgun sequence.
ACCESSION   DASZOB010000375 DASZOB010000000
VERSION     DASZOB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128803"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1506)
                     /locus_tag="VG709_05990"
     CDS             complement(<1..>1506)
                     /locus_tag="VG709_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWC32663.1"
                     /translation="RDPLTGLRNYRDFHAALDREIERARRHGGEFSVVLLEAEGFAEI
                     NRARGHVEGDRVLREVGRAVAGVCRACDLAARVGGDKLALMLPETGEQGAEELTQRVR
                     RAVAGIEEGLSLSHGIASWPAVGDSKELLLRRADVSLHLTKPSRARRGSDADGLAGEV
                     RLRVERVLALARDQLRMDVAFMGELVDGREVFRAVVGDGAAFGVETGAELALSETLCD
                     RMLNGRIPNIVADSATEPEIAGLAAVRDGGIGSYVGVPLHFPDGRLYGALCTLSADPQ
                     PDPSEREVELMRFLAGVVGELLDQAEAKAREHRAELELSGIRALVAALKARDHYTGEH
                     SRTVVRLATAVAERLGLSRAEVLEVEQLAMLHDIGKVGIPDSVLQKRAALSGEEWELM
                     LQHPAVGERIVGTIDSLSRLAPAVRAEHERFDGAGYPDRLERDEIPIASRITFVCDAY
                     HAMTSDRPYRAAMSPTEAQREVEANAGTQFDPVVVRALDAVLDEERRAGAR"
BASE COUNT          211 a          548 c          529 g          218 t
ORIGIN      
        1 ctcgcgcacc tgcgcgccgc tcctcatcga gcacggcgtc cagcgcccgg acgacgaccg
       61 ggtcgaactg ggttcccgcg ttggcctcga cttcgcgctg cgcctccgtc ggggacatgg
      121 cggcccggta cgggcgatcg ctcgtcatcg cgtggtaggc atcgcagacg aacgtgatcc
      181 ggctggcgat cggaatctcg tcgcgctcga ggcggtcggg gtatcccgct ccgtcgaagc
      241 gttcgtgctc ggcgcggacg gccggggcga ggcgggagag gctgtcgatg gtgcccacga
      301 tccgctcgcc caccgcgggg tgctggagca tcagctccca ctcctctccg ctcagcgcag
      361 cgcgtttctg gaggaccgag tcggggatcc cgacctttcc gatgtcgtgg agcatggcga
      421 gctgctcgac ctcgagcacc tccgcgcggg atagcccgag acgctcggcc accgcggtgg
      481 cgaggcggac gacggtgcgc gagtgctccc ccgtgtagtg gtcgcgagcc ttgagcgcgg
      541 ccacgagggc gcggattccc gacagctcga gctcggcgcg atgctcgcgc gccttggcct
      601 ccgcctggtc gagcagctcc ccgacgaccc cggcgaggaa ccgcatcagc tcgacctccc
      661 gctctgaggg gtccggttgc gggtcggcgc tcagcgtgca aagcgcgccg tacaggcggc
      721 cgtcggggaa gtgcagaggg acgcccacgt agctgccgat gcccccgtcg cggacggccg
      781 ccagcccggc gatctcgggc tcggtggccg agtcggcgac gatgttcggg atccggccgt
      841 tcagcatgcg gtcgcacagc gtctccgaga gggcgagctc tgctccggtc tcgacaccga
      901 aggcggcgcc gtctccgacg acggcgcgaa agacctccct tccgtcgacg agctcgccca
      961 tgaacgcgac atccatccgg agttggtcgc gcgccagagc gaggacgcgc tcgacgcgaa
     1021 gccgcacctc gcccgcgagc ccgtccgcgt cggagcctcg gcgagcccga ctcggcttgg
     1081 tcaagtgcag agacacgtcc gcccgtctca agagaagctc cttcgagtcg cccactgcgg
     1141 gccaggaggc gatgccgtgc gagagcgaca gtccttcctc gattccggcg accgcgcgcc
     1201 ggacgcgctg ggtcagctcc tccgcaccct gctcaccggt ctccgggagc atgagcgcga
     1261 gcttgtcgcc gccgacgcga gccgctaggt cgcacgcgcg gcacacgccc gcgacggctc
     1321 gcccgacctc gcgcaggacc cgatctccct ccacatggcc ccgagcgcgg ttgatctcgg
     1381 cgaacccctc ggcttccagg agaacgacgc tgaattcgcc gccgtgacgc cgcgcccgct
     1441 cgatctcgcg gtcgagggcg gcgtggaagt cgcgatagtt gcgaagcccg gtgagcgggt
     1501 cacgga
//
LOCUS       DASZOB010000376         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78526, whole genome shotgun sequence.
ACCESSION   DASZOB010000376 DASZOB010000000
VERSION     DASZOB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78526"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..>1517)
                     /locus_tag="VG709_05995"
     CDS             complement(149..>1517)
                     /locus_tag="VG709_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HWC32664.1"
                     /translation="AEALYYLTPRAKVHVPAPPTMWNHGNYVASVAEVARWLAERAEE
                     LGVTIVPETAATKLLVDAGRVVGVRTGDRGRGRDAQELANFEPGSDLMARVTILAEGT
                     QGHLAGVAMRRFGLATRPQVWALGVKEVWEVERPLGRVMHTMGWPLRGGRRYREFGGS
                     FVYPMGEGMVALGMVVGLDYTDATLSVHDLLQEFKGHPRIRRLLEGGRRIAWGAKTNP
                     EGGFLALPERFHFPGGMIVGDSAGFVNVPALKGIHYAMRSGMLAAEPAVDAVRPGSTA
                     WTPGALEGYDRAVRASYIWKDLERVRNMRPAFRYGFVRGALVAGAALNSLGRLPPRDL
                     SLEPDSEAELFIGERDRSYPKPDGKLFFDKLSSVYLSGNRTRDDAPNHIRIDRKVRRE
                     VAEAWVHMCPAQVYEIAGPASPAGNVVTVDVTPSNCIQCGAITAKGGRLTPPEGGDGP
                     DYKLM"
BASE COUNT          212 a          557 c          536 g          212 t
ORIGIN      
        1 gtgcaggcgg ttcccgatcg ccgcggacgc agcgacgacg tcgagcgcgg caccgtcccg
       61 cgcgagatcc gtcacgcttc cgtatcgggg tctgacccca gcacggaacg cgcgcggaag
      121 gtgcgcgaaa ggcgcacgcc ggcggcgctc acatcagctt gtagtcgggg ccgtcgccgc
      181 cctcgggcgg cgtcaggcgg ccgcccttcg ccgtgatcgc gccgcactgg atgcagttcg
      241 acggcgtcac gtcgacggtg acgacgttgc cggccgggct cgccgggccg gcgatctcgt
      301 acacctgcgc ggggcacatg tgcacccatg cctcggcgac ctcacggcgc accttccggt
      361 cgatgcggat gtggttgggc gcgtcgtcgc gcgtgcggtt gccgctcagg tagacgctcg
      421 agagcttgtc gaagaagagc ttcccgtccg gcttcggata gctccggtcc cgctcgccga
      481 tgaagagctc ggcttcggaa tcgggctcga gcgaaaggtc gcggggcggc aggcggccga
      541 gcgagttcag agcggcgccg gcgacgagcg ccccgcggac gaacccgtag cgaaaggccg
      601 ggcgcatatt ccggacgcgc tcgaggtcct tccagatgta gctcgcgcgc acggcgcggt
      661 cgtagccttc gagcgcgccc ggcgtccagg ccgtgctccc cggtcgcacg gcgtcgacgg
      721 ccggctccgc ggcgagcatg ccggaccgca tcgcgtagtg gatgcccttg agggcgggga
      781 cgttcacgaa ccccgccgag tcgccgacga tcatcccgcc tggaaagtgg aagcgctcgg
      841 gcagcgcgag gaacccgcct tccgggttcg tcttcgctcc ccaagcgatg cgcctcccgc
      901 cctcgaggag gcgccggatc cgcggatgcc ctttgaactc ctgcagaagg tcgtgcacgg
      961 aaagtgtcgc gtccgtgtag tcgagcccga ccaccattcc gagcgcgacc atgccctcgc
     1021 ccatcgggta gacgaagctc ccgccgaact cgcggtagcg ccgccccccg cgaagcggcc
     1081 agcccatcgt gtgcatcacg cgcccgagcg ggcgctcgac ctcccagacc tccttcacgc
     1141 cgagcgccca gacctgcggc cgcgtggcca ggccgaagcg ccgcatcgca acgcccgcga
     1201 ggtggccctg cgtgccctcg gcgaggatcg tcacgcgcgc catcaggtcg gagccgggct
     1261 cgaagttcgc gagctcctgt gcgtcgcgcc cacgcccgcg gtcgccggtc cggacgccga
     1321 cgacgcggcc ggcgtcgacg aggagcttcg tcgcggccgt ctccgggacg atcgtgacgc
     1381 cgagctcctc ggcccgctcg gcaagccagc gtgcgacctc ggcgaccgac gcgacgtagt
     1441 tcccgtggtt ccacatcgtc ggcggggcgg ggacgtgaac cttcgcccgc ggcgtcaggt
     1501 agtacagggc ctccgcc
//
LOCUS       DASZOB010000377         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78540, whole genome shotgun sequence.
ACCESSION   DASZOB010000377 DASZOB010000000
VERSION     DASZOB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78540"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1300)
                     /locus_tag="VG709_06000"
     CDS             complement(113..1300)
                     /locus_tag="VG709_06000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWC32665.1"
                     /translation="MTAMALEGIRVLDFTQMMVGPWGTQFLGDMGAEVVKVERPLTGE
                     WERGLRAMGELLEGESPYFLAMNRNKRSVTLNLKEPRARDIVLRLAERSNLVVENFRP
                     GVMDRLGMGYEDLRGVNPSIVYVAATGYGPDGPYVARPGQDLLIQAMSGLAAYGGRKG
                     DPPTPSGSSIVDASTALLLAFSAMAGLFHQQRTGEGQRIDVSLYGTAIALQCQELAIH
                     MNMARRFERSEAGIGGAWLSAPFGIYRTADGHMAVAMASLAVVAELLDVPELAQYDPP
                     DRAYDDRDEAYRLVQSRLLERTTAEWLETLATRDVWCAPVQTFDDVVEDPQVEHQEWI
                     TTVEHPNGRKLRVVGVPMRFSATPGTIRSGPPAVGQHTDEVLTDAGYSDEEIRSFHED
                     GVV"
     gene            complement(1297..2433)
                     /locus_tag="VG709_06005"
     CDS             complement(1297..2433)
                     /locus_tag="VG709_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWC32666.1"
                     /translation="MTEVVLRGITWNHDRGLGGFVATAGAWSERERDVRVEWTTRSLQ
                     AFADEPVEVLAERFDLLVIDHPSIGHAVATGSFVPLDDHVDSTFLDDQATHSVGASHR
                     SYEWDGHQWALAVDAASQVAAYRPDLLERAGATPPRTWDDVFELAGEGVPIALPSIPV
                     DAACAFFGVSGAPDGQPPSREGFLEALGLLRRILSVAHPESLAWNPPTVFERMATTDD
                     VAYVPLAFCYSNYARPGYRSNVVRFAAPPGRGTLGGAGLAVSARSRHVDEAVRYATFV
                     ADGETQRTTYFEGGGQPGHRSSWSDASVNAASSGFFADTLGALDRAYLRPRHDGFLTY
                     QERAADTIHGWLRGAGDDADTVVSELERAYRDSVPIDAETGASA"
     gene            complement(2430..3317)
                     /locus_tag="VG709_06010"
     CDS             complement(2430..3317)
                     /locus_tag="VG709_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWC32667.1"
                     /translation="MTTTDLGAALLAEEAGRVFFDGTLTDPQLDHPEGVAVHPDGSIW
                     CGGERGQIYRIDPDGTSIEEVASTGGFCLGMAFDADATRLFVCDLKHAAVFRLDVASR
                     RIERFADGAPAGRFRVPNYPAFGPDGALYVSDSHEFRAPGPGIWRVDPDGSARLWYAD
                     SVDFANGLAFSRDGRHVYVAETFGNAVFRLPVLDDGSPGDREEVASLPGAWPDGLAFD
                     TSGNVYVGCYEPSQILRIDPTGDVRLLYREMSAHTLAHPTNIAFRGSTLFTANLGRWH
                     VTAIDVGMEGAPLPIGASA"
     gene            3382..>4145
                     /locus_tag="VG709_06015"
     CDS             3382..>4145
                     /locus_tag="VG709_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWC32668.1"
                     /translation="MAVGIFRLSLPDSSIRLATGEASEGPARLLPEGVSLDDLVATGR
                     LDDVMDADARATEPVPDGARVLAPVGSQEVWAAGVTYLRSRDARVEEAVERTPYDRVY
                     EAERPELFFKSAGWRVRGPGERIAIRADSYWDVPEPELTLVLDGSARVVGYTIGNDVS
                     SRSIEGENPLYLPQAKTYDGSCSVGPCLVPAGSTAPPFSIVLEVVRDGDTAFRGETST
                     ERMRRSFEELAGYLGRALSFPSGAILLTGTGIVPDPP"
BASE COUNT          585 a         1507 c         1440 g          613 t
ORIGIN      
        1 gcctcgagcc ggtcgagcat ggcgctgctg atcgcgttga gcttgtcggg gcggttgaac
       61 gtggcgaccg cgacccggtc gcgtcggtcg acgaggagcg gctccccctt ggtcacacga
      121 cgccgtcctc gtggaacgag cggatctcct cgtcggagta cccggcgtcc gtgagcacct
      181 cgtcggtgtg ctggccgacc gccggaggcc ccgaccggat cgttccgggc gtggcgctga
      241 accgcatcgg gacgccgacc acgcgcagct tgcgcccgtt cggatgctcg acggtggtga
      301 tccactcctg gtgctcgacc tgtgggtcct cgacgacgtc gtcgaaggtc tggaccggcg
      361 cgcaccagac gtcgcgcgtc gcgagggtct ccagccactc ggcggtggtg cgctcgagca
      421 gccgcgactg cacgagccgg tacgcctcgt cgcggtcgtc gtacgcgcgg tctggcgggt
      481 cgtactgcgc gagctccggc acgtcgagga gctcggcgac gaccgcgagc gacgccatcg
      541 cgaccgccat gtgcccgtcc gcggttcggt agatcccgaa cggcgccgac agccacgccc
      601 ccccgatgcc ggcctcgctc cgctcgaagc gcctcgccat gttcatgtgg atcgcgagct
      661 cctggcactg gagcgcgatc gcggtgccgt agaggctcac gtcgatccgc tgcccctcgc
      721 ccgtccgctg ctggtggaac aggccggcca tcgcggagaa cgcgagcagc agcgcggtgc
      781 tggcgtcgac gatcgacgag ccggatggcg tcggaggatc ccccttgcgg ccgccgtagg
      841 ccgcgagccc gctcatcgcc tggatgagca ggtcctgtcc cgggcgcgcg acgtacggcc
      901 cgtcggggcc gtacccggtc gccgcgacgt agacgatgga cgggttcacc ccccgcaggt
      961 cctcgtaccc catgccgagg cggtccatca cgcccggccg gaagttctcg acgacgaggt
     1021 tcgaccgctc cgcgaggcgc agcacgatgt cgcgtgcacg tggttccttg aggttgagcg
     1081 tcacgctcct cttgttgcgg ttcatcgcga ggaagtacgg gctctcgccc tcgagcagct
     1141 cgcccatggc gcgcagcccc cgctcccact cgccggtgag tgggcgctcg accttcacga
     1201 cctcggcgcc catgtcgccg aggaactgcg tcccccacgg ccccaccatc atctgggtga
     1261 agtcgaggac acggatacct tccagcgcca tcgcggtcat gcgctcgctc ccgtctcggc
     1321 gtcgatcggc acgctgtcgc ggtacgcccg ttcgagctcg ctcacgacgg tgtcggcgtc
     1381 gtcgccggcc ccgcgcagcc acccgtggat cgtgtcggcc gcgcgctcct ggtacgtcaa
     1441 gaagccgtcg tgacgcggcc tcaggtacgc gcggtcgagc gcgccgagcg tgtcggcgaa
     1501 gaacccggaa gacgcggcgt tcaccgacgc gtcgctccac gacgaccgat gtcccggctg
     1561 gccgccgcct tcgaagtacg tcgtccgttg ggtctcgccg tcggccacga aggtcgcgta
     1621 ccggacggct tcgtcgacgt ggcgcgatcg ggccgagacc gcgaggcccg cgccgccaag
     1681 cgtcccgcgc cccggtggag cggcgaaccg cacgacgttc gaacggtaac ccggccgcgc
     1741 gtagttcgag tagcagaacg cgaggggcac gtacgccacg tcgtcggtcg tcgccatgcg
     1801 ctcgaacacc gtcggcggat tccacgcgag gctctccggg tgcgccaccg acaggatccg
     1861 tcgcaggaga cccagcgctt cgaggaaccc ctcgcgcgac gggggctggc cgtccggagc
     1921 gcccgagacc ccgaagaacg cgcacgccgc gtcgacgggg atcgacggca gcgcgatcgg
     1981 aacgccctcg ccggccagct cgaagacgtc gtcccacgtg cggggcggcg tcgctccggc
     2041 tcgctcgagg agatccggcc ggtacgcggc cacctgcgac gcggcgtcga ccgccagcgc
     2101 ccactggtgg ccgtcccact cgtagctccg gtggctcgcg ccgacgctgt gcgtcgcctg
     2161 atcgtcgagg aaggttgagt cgacgtggtc atcgaggggg acgaacgatc cggtcgcgac
     2221 ggcatggccg atcgacggat ggtcgatcac gaggaggtcg aagcgctcgg ccagaacctc
     2281 gaccggctcg tcggcgaatg cctggagcga gcgcgtcgtc cactcgaccc ggacatcccg
     2341 ctcgcgctcg ctccacgccc cggcggtcgc gacgaacccg cccaacccac ggtcgtggtt
     2401 ccacgtgatc ccgcgaagga cgacttcggt cacgcgctcg ccccgatcgg aagcggggcg
     2461 ccctccatcc cgacatcgat cgccgtcacg tgccagcgcc cgaggttcgc ggtgaacagc
     2521 gtcgaccctc ggaatgcgat gttcgtcggg tgcgccaggg tgtgggcgga catctctcgg
     2581 tacagcagcc gcacgtcgcc ggtcggatcg atccggagga tctggctcgg ctcgtagcag
     2641 ccgacgtaga cgttgcctga ggtgtcgaag gcgagaccgt ccggccatgc gcccgggagc
     2701 gatgcaacct cctcccgatc acccggagag ccgtcgtcga ggacggggag gcggaacacc
     2761 gcgttcccga acgtctcggc gacgtagacg tggcggccgt cacgcgagaa ggccagcccg
     2821 ttggcgaagt cgacggagtc cgcgtaccag agcctcgccg agccgtccgg atcgacccgc
     2881 cagatccccg ggccgggggc gcggaactcg tggctatcgc tgacgtaaag ggcgccgtcg
     2941 ggaccgaacg cggggtagtt cgggacgcgg aagcgccccg ccggtgcgcc gtcggcgaac
     3001 cgctcgatgc gccgcgacgc cacgtcgagc cggaacaccg ccgcatgctt caggtcgcag
     3061 acgaagagcc tcgtcgcgtc ggcgtcgaac gccatcccga ggcagaaccc gccggtcgac
     3121 gcgacctcct cgatcgaggt gccgtccggg tcgatgcggt agatctgccc gcgctcgccg
     3181 ccacaccaga tgctcccgtc ggggtgcacc gcgacgcctt cggggtggtc gagctgcgga
     3241 tcggtgagcg tcccgtcgaa gaacacgcgc ccggcttcct ctgcgaggag cgccgcgccg
     3301 aggtccgtcg tcgtcaccgg tcccgtgacc gtactcgctg cgcgcgggcg cgtgtcaaat
     3361 cgatttggct atcgttgccg cgtggccgtc ggcatcttcc gcctctcgct tcccgactcg
     3421 agcatccgtc tcgcgacggg cgaggcctcg gaaggacccg cgcggctcct ccccgagggc
     3481 gtctcgctcg acgatctcgt cgcgaccggc cggctcgacg acgtcatgga tgccgacgcg
     3541 agagcgaccg agccggtgcc ggacggcgcc cgggtcctcg ctccggtcgg atcgcaggag
     3601 gtgtgggcgg ccggcgtgac ctacctcagg tcgcgcgacg cgagggtcga agaagccgtc
     3661 gagcggacgc cgtacgaccg cgtgtacgag gccgaacgac ccgagctgtt cttcaagtcg
     3721 gccggctggc gcgtgcgcgg ccccggcgag cgcatcgcaa tccgagccga ctcttactgg
     3781 gacgttcccg agccggagct cacgctggtc ctcgacggct ccgcgcgcgt cgtggggtac
     3841 acgatcggca acgacgtctc ctcgcgcagc atcgaggggg agaacccgct ctacctcccg
     3901 caggccaaga cctacgacgg ctcgtgctcg gtcggtccct gcctcgtccc cgccggttcg
     3961 accgcgccgc cgttctcgat cgttctggag gtcgtccgcg acggcgacac ggccttccgc
     4021 ggcgagactt cgacagaacg catgcgtcga agcttcgagg agctcgccgg ctacctcggg
     4081 cgcgcgctct ctttcccctc cggcgcgatc ctgctcaccg ggaccgggat cgtgcccgat
     4141 ccgcc
//
LOCUS       DASZOB010000378         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_28459, whole genome shotgun sequence.
ACCESSION   DASZOB010000378 DASZOB010000000
VERSION     DASZOB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28459"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VG709_06020"
     CDS             complement(<1..546)
                     /locus_tag="VG709_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWC32669.1"
                     /translation="MAVDQTHERDVVLDIEGMTCASCVNRIERVLQRHEGVAAARVNL
                     ASHTATVRTTLAAPDALIRAVERAGYGARPHERGLDARGEGADYARRLVVSAALTVPI
                     LALTFGVGASWATWAAWALATPVQFYGGWPFIRAAARAARHGTSTMDTLVAVGSLAAY
                     GYSAWAVATDVEGHYFDTAAVI"
     gene            complement(564..782)
                     /locus_tag="VG709_06025"
     CDS             complement(564..782)
                     /locus_tag="VG709_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HWC32670.1"
                     /translation="MHELRLKVEGMDCTGCEASIGKAVSAVPGVADVSAEHATGAVRV
                     TAEGALDEVAVRRAIEDAGYDVVGAESG"
     gene            854..1147
                     /locus_tag="VG709_06030"
     CDS             854..1147
                     /locus_tag="VG709_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWC32671.1"
                     /translation="MKTPTRPGYVEDREDVLRRLRRIEGQVRGLQRMVEEERYCVDVL
                     AQISSATSALEKVGLVLLNDHIRHCVRGALADGGGDDKVEELVTAVDRFLRAT"
     gene            1147..>2357
                     /locus_tag="VG709_06035"
     CDS             1147..>2357
                     /locus_tag="VG709_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWC32672.1"
                     /translation="MDHREHETHAQDAPEHAAHAAGHPGKHGGGHDRHAGHSVEMFRN
                     RFWVSLVLTIPVLAYAEMLWEWVGLRPPDLPAPKVVPFVVATAIYLYGGIVFLRSAAA
                     EIRARRPGMMTLVAVGITAAWAYSTATTFVLEGEGFYWELATLVDVMLLGHWIEMRSI
                     GRASGALAELAKLLPDTAERVTDDGTEEVSVADLETGDVVLVRPGARVPADGEVADGN
                     SHVNEAMITGESKPVRKSPGERVVAGTVNQEGSLRVRVTATGESTALAGIMRLVEEAQ
                     ASKSGAQAVADRVAAALTYVALAAGTITRAVWLAATARDTSFAVERAVTVIVIACPHA
                     LGLAVPLVVAISTTLSARSGLLVRDRLALERARTIDTVVFDKTGTLTLGEMGLVGVAM
                     ITGDSDDVARWV"
BASE COUNT          316 a          870 c          853 g          318 t
ORIGIN      
        1 gatgacggct gcggtgtcga agtagtggcc ctcgacgtcc gtggcgaccg cccacgcgct
       61 gtatccgtac gccgcgagcg accccaccgc gaccagcgtg tccatcgtcg aggtgccgtg
      121 ccgggcggcg cgagcggccg cccggatgaa cggccagccg ccgtagaact ggacgggggt
      181 ggcgagcgcc cacgctgccc acgtcgccca cgacgcgccg acgccgaacg tgagcgcgag
      241 gatcgggacg gtcagcgcgg cggacacgac gaggcggcgt gcgtagtcgg cgccctcgcc
      301 ccgcgcgtcg aggccgcgct cgtgcgggcg cgccccgtag ccggcgcgct ccaccgccct
      361 gatcagggcg tccggcgcgg ccagcgtcgt ccgaaccgtc gcggtgtgcg acgcgagatt
      421 gacgcgcgcg gcggcgacgc cctcgtggcg ctgcagcacc cgctcgatcc ggttcacgca
      481 cgacgcgcac gtcatcccct cgatgtcgag gacgacgtcc cgctcgtgcg tctggtcgac
      541 ggccacgtcg ctcgccccgc cgctcagccg gactccgccc cgacgacgtc gtagcccgcg
      601 tcctcgatcg cgcggcggac ggcgacctcg tcgagcgcgc cctcggcggt cacgcggacg
      661 gccccggtcg cgtgctccgc cgacacgtcc gcgacccccg gcacggcgga gaccgccttg
      721 ccgatgctcg cctcgcaccc cgtgcagtcc atcccctcga ccttcagcct cagctcgtgc
      781 atctgccctc cttcttgccg ttccggttcc gagcggatac cctacggggg tatcctacac
      841 cggaggtcga ccattgaaga cacccacccg tccaggctac gtcgaggatc gcgaagacgt
      901 gctccgccgg ctccgccgga tcgagggaca ggtgcgcgga ttgcagcgga tggtcgagga
      961 ggagcgctac tgcgtcgacg tgctcgctca gatctcgtcc gcgacgagcg cgctggagaa
     1021 ggtcgggctg gtgctgctca acgaccacat ccgccactgc gtccgcggcg ccctcgccga
     1081 cggcggcggt gacgacaagg tggaggagct cgtcacggcc gtcgaccgct tcctacgggc
     1141 gacgtgatgg accaccgcga acacgagaca cacgcgcagg acgcccccga gcacgctgcg
     1201 cacgcggccg gccaccccgg gaagcacgga ggaggccacg accgccacgc cggccactcc
     1261 gtggagatgt tccgcaaccg cttctgggtc tccctcgtcc tgacgatccc cgtcctcgcg
     1321 tacgccgaga tgctgtggga gtgggtcggc ctgcggccgc cggacctccc ggcgccgaag
     1381 gtcgtcccgt tcgtcgtggc aaccgcgatc tacctgtacg gcgggatcgt gttcctccgc
     1441 tccgccgcgg cggagatccg cgcgcgccgg ccgggcatga tgacgctcgt cgcggtcggg
     1501 atcaccgcgg cgtgggcgta cagcacggcg accacgttcg tcctcgaggg cgagggcttc
     1561 tactgggagc tcgccacgct cgtcgacgtg atgctgctcg gccactggat cgagatgcgc
     1621 tcgatcggcc gggcctcggg ggcgctcgcg gagctggcga agctcctgcc cgacacggcg
     1681 gagcgcgtca ccgacgacgg taccgaggag gtctcggtgg cggacctcga gacgggcgac
     1741 gtcgtgctcg tccgcccggg cgcgcgggtc ccggccgacg gcgaggtggc ggacgggaac
     1801 tcgcacgtca acgaggcgat gatcaccggc gagtcgaagc ccgtccggaa gtcgccgggc
     1861 gagcgggtcg tcgccggcac ggtgaaccag gagggctcgc tccgcgtccg cgtgaccgcg
     1921 accggggagt cgaccgcgct cgccgggatc atgcggctcg tcgaggaggc gcaggcgtcg
     1981 aagtccggcg cgcaggcggt cgcggaccgc gtcgccgccg cgctgaccta cgtcgcgctg
     2041 gcggccggaa cgatcacgcg cgccgtctgg ctggccgcga cggcgcggga cacgtcgttc
     2101 gccgtcgaac gggcggtgac ggtcatcgtc atcgcctgcc cgcacgcgct cgggctcgcg
     2161 gtgccgctcg tggtcgcgat ctcgacgacg ctgtccgcgc ggagcgggct cctcgtccgc
     2221 gaccgcctcg cgctcgagcg cgcgaggacg atcgacaccg tcgtgttcga caagaccggc
     2281 acgctgacgc tcggagagat gggcctcgtc ggcgtcgcga tgatcacggg cgatagcgac
     2341 gacgtcgcgc gctgggt
//
LOCUS       DASZOB010000379         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_28516, whole genome shotgun sequence.
ACCESSION   DASZOB010000379 DASZOB010000000
VERSION     DASZOB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28516"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VG709_06040"
     CDS             complement(<1..182)
                     /locus_tag="VG709_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWC32673.1"
                     /translation="MSWDHERVEELLAGYVLGGLDDEDAALASRAVLEHVPECERCRR
                     SLEEYRLVAGDLGLAAP"
     gene            complement(179..724)
                     /locus_tag="VG709_06045"
     CDS             complement(179..724)
                     /locus_tag="VG709_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC32674.1"
                     /translation="MAETDLELLGRVAAREAAAFEQLYRRYAAAAYGLAVRVLRQPAL
                     AQEAVQEAFVTVWNAPEAFDPSRGPFRSFLLSLVHHRAVDAVRREERLRERERRANPE
                     PVTDEDVMEVIAEEADLADRRRQVRDAMRELPPAQREMLELMYFRGWSHARIARETGV
                     PIGTVKSRIWAAMRRLREALA"
     gene            776..1024
                     /locus_tag="VG709_06050"
     CDS             776..1024
                     /locus_tag="VG709_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWC32675.1"
                     /translation="MATVTLYSRRTCGLCDEARAEIEALRSEIPFELDEVLIDGRDDL
                     ERAYGIRVPVVLVDGAEAFEIHVDREELRALVARSSGA"
     gene            1057..1782
                     /locus_tag="VG709_06055"
     CDS             1057..1782
                     /locus_tag="VG709_06055"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWC32676.1"
                     /translation="MASRAAVRTMTTRQIPEATVGRLPLYLRALVEMAERGQTTISSE
                     GLAEAAGVNSAKVRKDLSYLGSYGTRGVGYDVAYLIHQIRRELGLTEDWPVLIAGVGN
                     LGHALANYKGFGERGFRVVALVDAAERKVGEIVAGTRVEHIDAMPEVVRRHDVVIGII
                     STPAGAAQEVADRMVAAGVRSILNFAPAVIAVPPEVSLRKVDLSIELQILGFYEQRKA
                     VLADLEDGRGRELAGVAELGSVG"
     gene            1786..>2432
                     /gene="hemA"
                     /locus_tag="VG709_06060"
     CDS             1786..>2432
                     /gene="hemA"
                     /locus_tag="VG709_06060"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWC32677.1"
                     /translation="MPVLALGVSYRRAPVDLLERLAVTREEEPKAYRRVLELGSVSEA
                     VLLSTCNRVEVYAEVDRYHQGFQDLKRFLAEDREVPVDEFAEPLYSHYEDQAAEHLFS
                     VASGLDSMGTGEPQILSQVRAALRTARAEHAAGQVLESLFTHAVRVGKRARAETAIGA
                     SPSAFVEAGARLAERFLGGLAGRSVVVVGSGEMAALAIDSLRARAVGDVVVVGRRA"
BASE COUNT          328 a          873 c          894 g          337 t
ORIGIN      
        1 ggcgcggcga gcccgaggtc gccggcgacg aggcgatact cctccagcga ccggcggcag
       61 cgctcgcact ccgggacgtg ctcgaggacg gcgcgggagg cgagcgcggc gtcctcgtcg
      121 tcgaggccgc ccagcacgta gccggccagg agctcctcga cgcgctcgtg gtcccagctc
      181 atgcgagcgc ctcgcgcaga cgcctcatcg cggcccagat cctgctcttc acggttccga
      241 tcggcactcc cgtctcccgc gcgatccgcg cgtggctcca ccctcggaaa tacatcagct
      301 cgagcatctc ccgctgggcg ggagggagct cgcgcatcgc gtcgcgcacc tggcggcgac
      361 ggtcggcgag atcagcctcc tcggcgatga cctccatgac gtcttcgtcc gtgaccggct
      421 ccgggttcgc ccggcgctcc cgctcgcgga gccgctcctc gcgcctgacg gcgtcgacgg
      481 cccggtgatg cacgagcgag agcaggaacg agcggaacgg cccgcgcgac gggtcgaacg
      541 cctccggggc gttccagacc gtgacgaacg cctcctgcac ggcctcctgg gcgagcgcgg
      601 gctgacgcag cacgcggacc gcgagcccgt acgcggccgc ggcgtaccgc cggtacagct
      661 gctcgaacgc ggcggcctcc cgcgccgcca cgcgcccgag gagctcgagg tccgtctccg
      721 ccacgccgac gagtatgacc gccgccggcg agtctgggcg ctatcctgcg ccgtcgtggc
      781 gaccgtgacg ctgtactcgc gcaggacgtg cgggctctgc gacgaggcca gggccgagat
      841 cgaggcgctc cgctcggaga tcccgttcga gctcgacgag gtcctgatcg acgggcgcga
      901 cgatctggag cgcgcgtacg ggatccgcgt ccccgtcgtg ctggtggacg gggccgaggc
      961 gttcgagatc cacgtcgacc gggaggagct gcgggcgctc gtcgcccgct cctccggagc
     1021 ttgagaattc gagaaaccgc acgtcggagg cgtagaatgg cgtcccgtgc cgcggttcgg
     1081 accatgacca cgaggcagat cccagaggcc accgtcgggc ggctcccgct ctacctaagg
     1141 gcgctcgtgg agatggccga gcgggggcag acgacgatct cctccgaggg gctcgccgag
     1201 gccgctggcg tgaactccgc caaggtccgc aaggacctct cctacctcgg gtcgtacggg
     1261 acgcgcgggg tcgggtacga cgtcgcgtac ctcatccacc agatcagacg cgagctgggg
     1321 ctgaccgagg actggccggt gctcatcgcc ggggtcggga acctcgggca cgcgctcgcg
     1381 aactacaagg gcttcggcga gcgagggttc cgcgtcgtcg cgctcgtcga cgccgccgag
     1441 aggaaggtcg gcgagatcgt ggcaggcacg cgcgtcgagc acatcgacgc gatgcccgag
     1501 gtcgtgcggc gccacgacgt ggtgatcggg atcatcagca cccccgccgg cgccgcgcag
     1561 gaggtggcgg accgcatggt ggccgcaggc gtgcgatcga tcctcaactt cgccccggcg
     1621 gtgatcgccg tcccgccgga ggtctcgctc cggaaggtcg atctctcgat cgagctccag
     1681 atcctcgggt tctacgagca gcgcaaggcc gtgctcgccg acctcgagga cggccggggg
     1741 cgcgagctcg ccggcgtcgc cgagctcggg tcggtcgggt agcccgtgcc ggtcctcgcc
     1801 ctcggcgtct cgtaccgccg tgctccggtc gacctcctcg agcggctggc ggtgacgcgc
     1861 gaggaggagc cgaaggccta ccgccgcgtg ctcgagctcg gctccgtcag cgaggccgtg
     1921 ctgctgtcca cgtgcaaccg cgtcgaggtg tacgcggagg tcgatcggta ccaccagggg
     1981 ttccaggacc tgaagcgctt cctcgccgag gaccgcgagg tccccgtgga tgagttcgcc
     2041 gagccgctct actcgcacta cgaggaccag gccgccgagc acctgttctc ggtcgcctcg
     2101 gggctcgact cgatggggac gggggagccc cagatcctct cgcaggtgcg cgcggcgctg
     2161 cgcaccgcgc gcgcggagca cgcggcgggg caggtgctcg agtcgttgtt cacgcacgcc
     2221 gtccgcgtcg ggaagcgggc ccgagccgag accgccatcg gcgcgagccc ctcggcgttc
     2281 gtggaggccg gcgcacggct cgccgagcgg ttcctcggag ggctcgccgg acgaagcgtc
     2341 gtcgtcgtcg ggtccggcga gatggccgcg ctcgcgatcg actcgctgcg cgcccgagcc
     2401 gtcggcgacg tcgtcgtcgt cggccggcgc gc
//
LOCUS       DASZOB010000380         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_8489,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000380 DASZOB010000000
VERSION     DASZOB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8489"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VG709_06065"
     CDS             <1..1048
                     /locus_tag="VG709_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWC32678.1"
                     /translation="RPADRAVRATQEAVETPEGNSWLPFTGKADLRRAVADQYETRSG
                     VAVDPDAQVVITCGEGDAMVDALFITTDPGDEVVVTDPTYAGMINRVRIVGAVPRFVP
                     HVPEGGEWRLDVDALQGAVSDRTRAIFLANPDMPTGRHLDDDEWEAIASICRERDVWL
                     IYVAWMERILFDGRPYRHPAALDGMADRVVHVGCVTCEQRMIGWRIGWLVTPPALAED
                     AQRAHIYNGLTPGGIAQAGALAALREGDAEIEDAVAEWQRRRDETLRQLQDLPVVRPA
                     GGWQLLMHAEALGTTPSELSRALVDQKVAATPMTAWGERVSPRFIRFVYSREPVERLA
                     LLGERLRSALDRLA"
     gene            1051..1989
                     /locus_tag="VG709_06070"
     CDS             1051..1989
                     /locus_tag="VG709_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWC32679.1"
                     /translation="MLAAVATQTEPRTPQGGGRAAAAASALTAAALWGGMYVVSKDTF
                     SEIPPITLGAVRLILAAAGLFAFLRARGPLRRPHDASLVVAGVLLAITMVVQFVGTDL
                     ATASAGAVLTTTTPLFLVPLAWVFLHERPGWKTVVAVAVGMAGVLLAVEGGEATARGP
                     WGAVLLLLSAATWAAYTIASAPVARRDGPLVAVAWACVYAVPIVLALSLFETGRWTVA
                     AFTDGSTMLAIAYLGLASGAAAWLLWIRGVAGLPAAVVGAFFFVQPIVGGALARVFLD
                     ERLTLRFAAGAAMILIGVVLALGGSRDAVPQREETR"
     gene            1986..2633
                     /locus_tag="VG709_06075"
     CDS             1986..2633
                     /locus_tag="VG709_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWC32680.1"
                     /translation="MTIQIVNEDVERYMRDLLGGEHAIATEMEAVAREREFPIVGRLV
                     GVTLEVLARSIGARRVLEMGSGYGYSAYWFARAVGDGGEVHMTEGDAQNQEQAMAYLR
                     RAGLAERVTSHVGNALEIVDELDGDFDVIFCDIDKLDYPAAWEKARERIRVGGLYLCD
                     NVLWSGHVTGKTDDDTRPDWTEAVREHNGAVASDERYVATIIPTRDGVMCAVRIS"
     gene            complement(2637..>4851)
                     /gene="leuS"
                     /locus_tag="VG709_06080"
     CDS             complement(2637..>4851)
                     /gene="leuS"
                     /locus_tag="VG709_06080"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWC32681.1"
                     /translation="LLHPIGWDSFGLPAENAAIKRGIHPKEWTYANIDKQRASFKRYG
                     VSFDWSRQFNTSDPEYYRWTQWLFLKLFERGLAYRKLAPANWCPKDQTVLANEQVVAG
                     ACERCGTPVVRKDLVQWFFRITDYAQALLEDMALLEGWSERVLTMQRNWIGRSDGAEV
                     RFPIAETGEDITVFTTRPDTLWGVTFFVFAPEHPLVGRLAEIGGVADEVGAMLDRLQA
                     TPLTSREQAESREGIALRVNAVNPVNGERVPCFVAPYVLMEYGTGAVMGVPGHDQRDF
                     EFAREHGLEVRVVIQPEGEEIRADEMSEAYPHEGVMVSSGPFDGTPSPKSIERVIDWL
                     EQERRGERAVSYRLRDWLISRQRYWGAPIPIIHCPRDGEVPVPEDELPVLLPDDVDFA
                     PTGESPLAAHEGFVSVACPRCGGDARRDTDTMDTFVDSSWYFLRYCSPHAEDAPFDRE
                     EVALWMPVDQYSGGIEHAILHLLYSRFFVKALHDMGLVPFREPFMRLLNHGMVIMSGS
                     KMSKSRGNLAEFGRELDMHGADVIRLTMLFANNPEDEIDWALVSSEGIESWLGRVWRA
                     VHDAVERSDEPDPEALRVFTHRTIKRVTDMFDRFRYNVAVARLMELTNEIRRTLDAGE
                     PAREAATALVLMLSPMAPFIAEELWRETLGGEGPVHRQAWPAFEPDLAREDEVVLVIQ
                     VDGKVRDRITVSADISEDEAVRLARESDRVKRTLDGREIRRVIARPPRLVNLVTS"
BASE COUNT          698 a         1733 c         1684 g          736 t
ORIGIN      
        1 ccgcccggcc gaccgtgccg ttcgagccac gcaggaggcc gtcgagaccc cggaaggcaa
       61 cagctggctt cccttcacgg ggaaggcgga cctgcgccgt gccgtggccg accagtacga
      121 aacgcgctcc ggcgtcgccg tcgaccccga cgcgcaggtc gtgatcacct gcggcgaggg
      181 cgacgcgatg gtcgacgccc tcttcatcac gaccgacccc ggcgacgagg tggtcgtcac
      241 cgatccgacg tacgcgggga tgatcaaccg cgtgcggatc gtcggcgcgg tccccaggtt
      301 cgtcccgcat gtcccggagg gcggcgaatg gcgcctggac gtcgacgcgc tacaaggagc
      361 ggtctccgac cggacgcgcg cgatcttcct ggcgaacccc gacatgccca ccggccgcca
      421 cctcgacgac gacgagtggg aggcgatcgc ctcgatctgt cgggagcgtg acgtgtggct
      481 catctacgtg gcgtggatgg agcggatcct gttcgacggc cgtccgtacc ggcaccccgc
      541 cgccctcgac gggatggcgg atcgcgtcgt ccacgtcggc tgcgtgacgt gcgagcaacg
      601 catgatcggg tggaggatcg ggtggctggt cacgccgccg gcgctcgcgg aggacgcgca
      661 gcgcgcccac atctacaacg ggctcacgcc cggcgggatc gcgcaggccg gagcgctggc
      721 ggcgctccgc gaaggcgacg cggagatcga ggacgccgtg gcggaatggc agcgccgtcg
      781 cgacgagacg ctgcgccagc tccaggacct cccggtcgtc cggcccgccg gcggctggca
      841 gctgctcatg cacgccgagg cgctcgggac cacgccgtcc gagctctcgc gcgcgctcgt
      901 cgaccagaag gtcgccgcga cgccgatgac cgcgtggggt gagcgcgtct cgccgcggtt
      961 catccgcttc gtctacagca gggagccggt cgagcggctg gcgctcctcg gcgagcggct
     1021 ccgctcggcg ctcgatcgcc tcgcgtgagg atgctcgccg cggtcgccac gcagaccgag
     1081 ccgcggacgc cacagggcgg cggccgagcc gcggccgccg cgtcggcgct cacggcggcg
     1141 gcgctctggg gcggcatgta cgtcgtcagt aaggacacgt tctcggagat cccgccgatc
     1201 acgctgggag ctgtccggct catccttgcc gcggccgggc tcttcgcctt cctccgcgcc
     1261 cgcggaccgc tgcggcgtcc gcatgacgcg agcctcgtcg ttgccggcgt gctgctggcg
     1321 atcacgatgg tcgtccagtt cgtcggcacc gatctcgcga cggcctcggc cggggcggtg
     1381 ctgacgacga cgacgccgct gttcctcgtc ccgctcgcct gggtgttcct ccacgagcgc
     1441 cccggctgga agaccgtcgt ggccgtggcg gtcggcatgg ccggcgtcct cctcgccgtc
     1501 gagggcggcg aggcgacggc gcgcggaccg tggggcgcgg tgctcctgct gctctccgcg
     1561 gcgacgtggg ccgcgtacac gatcgcgtcg gcgccggtcg cgcgtcgcga cggtccgctg
     1621 gtggcggtcg cgtgggcgtg cgtctacgca gtaccgatcg tgctcgcgct gtcgctgttc
     1681 gagacgggtc gatggaccgt cgcggcgttc accgacgggt ccacgatgct cgcgatcgcc
     1741 tatctcggcc tggcgtccgg cgccgcggcc tggctgctgt ggatccgggg cgtcgcgggt
     1801 ctcccggccg cggtcgtcgg agcgttcttc ttcgtccagc ccatcgtcgg cggagcgctc
     1861 gcacgcgtct tcctcgacga gcgcctaacc ctccgcttcg ccgccggcgc agcgatgatc
     1921 ctgatcggcg tcgtgctcgc gctcggcggg agccgcgacg cagttcccca acgagaggag
     1981 acgagatgac gatccagatc gtgaacgagg acgtcgagcg ctacatgagg gacctcctcg
     2041 gcggcgagca cgcgatcgcg accgagatgg aggcggtcgc gcgcgagcga gagttcccga
     2101 tcgtcggccg cctcgtcggc gtgacgctcg aggtcctggc ccgttcgatc ggcgcccgac
     2161 gggtcctcga gatgggatcc ggctacggct actcggcgta ctggttcgcg cgcgcggtcg
     2221 gcgacggcgg cgaggtgcac atgaccgagg gcgacgcaca gaaccaggag caggcgatgg
     2281 cgtaccttcg ccgcgccggg ctggccgagc gcgtgacgag ccacgtcggg aacgcgctcg
     2341 agatcgtcga cgagctcgac ggggacttcg acgtgatctt ctgcgacatc gacaagctcg
     2401 actacccggc ggcgtgggag aaggcgcgcg agcggatccg cgtcggcggc ctctacctct
     2461 gcgacaacgt gctctggagc ggccacgtca cggggaagac cgacgacgac acccggccgg
     2521 actggacgga ggccgtacgc gagcacaacg gggccgtcgc ctccgacgag cgctacgtcg
     2581 ccacgatcat cccgacccgc gacggcgtca tgtgcgccgt tcggatctcc tgagcgctac
     2641 gacgtcacca ggttgacgag tcgaggcggg cgcgcgatca cccggcggat ctcccggccg
     2701 tcgagcgtgc gcttcacgcg gtccgactcc ctcgccagcc ggaccgcctc gtcctcggag
     2761 atgtccgccg agaccgtgat gcggtcgcgg accttgccgt cgacctggat cacgagcacg
     2821 acctcgtctt cacgggccag gtccggctcg aacgccggcc atgcctgccg gtgcaccggc
     2881 ccctcgccgc cgagcgtctc acgccagagc tcctccgcga tgaacggtgc catcggcgag
     2941 agcatcagca ccagcgccgt ggcggcctcg cgggcgggtt cgccggcgtc gagcgtcctc
     3001 cggatctcgt tcgtcagctc catcagccgc gcgaccgcga cgttgtaacg gaaccggtcg
     3061 aacatgtcgg tcacgcgctt gatcgtccgg tgcgtgaaca cgcgcagcgc ttcgggatcg
     3121 ggctcgtcgg agcgctcgac ggcgtcgtgg accgcccgcc acacgcgtcc gagccaggac
     3181 tcgatcccct cggacgagac cagcgcccag tcgatctcgt cctcgggatt gttcgcgaac
     3241 agcatggtga gccggatgac gtccgcgccg tgcatgtcga gctctcgccc gaactccgcg
     3301 aggttccccc ggctcttgct catcttggac ccgctcatga tgaccatgcc gtggttgagg
     3361 agacgcatga acggctcgcg gaacggcacg agccccatgt cgtggagcgc cttaacgaag
     3421 aaccgcgagt acaggagatg caggatcgcg tgctcgatcc ctccgctgta ctggtcgacg
     3481 ggcatccaca gcgcgacctc ttcgcggtcg aacggcgcat cctcggcgtg cggcgagcag
     3541 taccgcagga agtaccagga agagtcgacg aacgtatcca tcgtgtcggt gtcgcggcgc
     3601 gcgtcgccac cgcagcgcgg gcacgcgacc gacacgaacc cttcgtgcgc cgccagcggg
     3661 ctctcccccg tcggcgcgaa gtcgacgtca tcggggagca gaaccgggag ctcgtcttcc
     3721 ggaaccggaa cctcgccgtc gcgagggcag tggatgatcg ggatcggggc cccccagtag
     3781 cgctgccgcg agatcagcca gtcccgcagg cggtacgaga ccgcgcgttc gcctctccgc
     3841 tcctgctcga gccagtcgat gacgcgctcg atggatttgg gcgacggcgt gccgtcgaag
     3901 ggaccggagg acaccatcac gccctcgtgc gggtaagcct cactcatctc gtcggcgcgg
     3961 atctcctcgc cttctggctg gatgacgacg cggacctcga gcccatgctc ccgcgcgaac
     4021 tcgaagtctc gctggtcgtg gccgggcacg cccatcacgg cgcctgttcc gtactccatc
     4081 aggacgtacg gcgcgacgaa gcacgggacc cgttcgccgt tcaccgggtt caccgcgttc
     4141 acccggagcg cgatgccctc gcgcgactcc gcctgctcgc ggctcgtgag gggtgtcgcc
     4201 tgcaggcggt cgagcatcgc gcccacctca tcggcgacgc cgccgatctc ggcgaggcga
     4261 ccgaccagtg gatgctccgg cgcgaagacg aagaacgtca cgccccacag cgtgtccggt
     4321 ctggtcgtga acaccgtgat gtcttcgccc gtctcggcga tcgggaagcg cacctccgca
     4381 ccgtccgacc ggccgatcca gttccgctgc atcgtgagga cgcgctcgga ccatccctcc
     4441 agcagcgcca tgtcctcgag caacgcctgc gcgtagtcgg tgatccggaa gaaccactgc
     4501 acgaggtcct tgcgcaccac gggggtcccg caccgctcac acgcgccggc gacgacctgc
     4561 tcgttcgcga ggaccgtctg gtccttcggg caccagttcg ccggcgccag cttccggtag
     4621 gcgaggcctc gctcgaacag cttcaggaac aaccactgcg tccagcggta atactcggga
     4681 tccgacgtgt tgaactgccg cgaccagtcg aacgacacgc cgtaccgctt gaacgacgcg
     4741 cgctgcttgt cgatgttcgc gtacgtccat tccttcgggt ggatcccgcg cttgatcgcg
     4801 gcgttctcgg cggggagccc gaacgagtcc cacccgatcg ggtgcagcaa g
//
LOCUS       DASZOB010000381         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_58681, whole genome shotgun sequence.
ACCESSION   DASZOB010000381 DASZOB010000000
VERSION     DASZOB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58681"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VG709_06085"
     CDS             <1..791
                     /locus_tag="VG709_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWC32682.1"
                     /translation="VEAVRRLPVQQAVFDGEALWMGDEGPAAFQETVSRIDSDAPPVG
                     TVTFLFDLLHVDGSDLLDTPLEERAAKLEAIAPQLRIPAVITSDPEEGERILDEALGA
                     GHEGVVVKDAASLYAAGRRGKAWRKLKPVRTYDLVVLGAEWGHGRRRGWLSNLHLGAR
                     DPVTGEFVMVGKCFKGLTDDLLAWQTKELLERETARRGIAVHVRPELVVEIALDGVQS
                     STRYPGGVALRFARVKRYRPDKASEEADTIDDLRALLPAARGAR"
     gene            802..1689
                     /gene="ligD"
                     /locus_tag="VG709_06090"
     CDS             802..1689
                     /gene="ligD"
                     /locus_tag="VG709_06090"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWC32683.1"
                     /translation="MPARRVRLPNPARLLFPDDGITKSDLFAYYDAVAPALVPHLRDR
                     PFTMKRYREGIGSSGFFQKQAPKGIPEWIRTRQFRTFPREGGSRLVDFPLVDPPEALL
                     WMVQMHCIDMNAWYSRVDKPHRPDFVVFDLDPPDGAFALAVRVAHLVRKALEELALRS
                     YVKTSGADGIHVLVPIARRSTFDETYEFAERVSRSLEERHPGLVTTEWLKRKRSGVLV
                     DHRQNGWGKTIASVYSVRPRPGAPVSTPLRWEELTEEVTPRQFGMQEALARVEREGDV
                     YEPVLRGRQALGPALRKLR"
BASE COUNT          254 a          604 c          612 g          257 t
ORIGIN      
        1 tcgtggaggc ggtgcgccgg cttcctgtcc agcaggccgt gttcgacggc gaggcgctgt
       61 ggatgggcga cgagggcccg gccgccttcc aggagaccgt ctcgcggatc gacagcgacg
      121 cgccgccggt ggggaccgtg acgttcctct tcgacttgct ccacgtggac ggctccgacc
      181 tgctcgacac gccgctcgag gagcgcgccg cgaagctgga ggcgatcgcg ccgcagctga
      241 ggatcccggc agtgatcacc tccgacccgg aagagggaga gcgcatcctg gacgaggccc
      301 tcggtgccgg gcacgagggc gtggtcgtga aggacgccgc gtcgctctac gcggccggcc
      361 gtcgcggcaa ggcttggcgc aagctgaagc cggtgcggac ctacgacctc gtcgtgctcg
      421 gtgccgagtg gggccacggg cgccggcggg gctggctctc gaacctgcac ctcggcgccc
      481 gcgatcccgt cacgggcgaa ttcgtgatgg tggggaagtg cttcaagggg ctgacggacg
      541 atctcctcgc ctggcagacg aaggagctgc tggagcgtga gaccgcccgg cgcgggatcg
      601 ccgtgcacgt ccgtcccgag ctcgtggtcg agatcgcgct ggacggcgtc cagtcgtcga
      661 cgcgctaccc gggcggcgtc gcgctgcgct tcgcccgcgt gaagcggtac cgaccggaca
      721 aggcgtcaga ggaagcggac acgatcgatg acctgcgcgc cctgctcccg gccgcgcgcg
      781 gcgcgcggta gaaccccgcc gatgcccgcg cggcgcgtac gcctcccgaa cccagcccgc
      841 ctcctcttcc ccgatgacgg gatcacgaag agcgatctgt tcgcgtacta cgacgccgtc
      901 gcgcccgcgc tcgtcccgca cctccgcgat cgcccgttca cgatgaagcg ctatcgcgag
      961 ggcatcggca gcagcggctt cttccagaag caggcgccga agggcatacc tgaatggatc
     1021 cggacgcgtc agtttcgtac cttcccgcgc gaaggcggct ctcgcctcgt cgacttcccg
     1081 ctcgtcgacc cgcctgaggc gctgctctgg atggtccaga tgcactgcat cgacatgaac
     1141 gcctggtact cgcgcgtcga caagccgcac cgtccggact tcgtcgtctt cgacctcgac
     1201 ccaccggacg gcgccttcgc cctcgccgtc cgcgtcgcgc acctcgtgcg gaaagcgctc
     1261 gaggagcttg cgctgcgctc gtacgtcaag acgagcggcg cggatggaat ccatgtcctc
     1321 gtcccgatcg cgcgccgctc gacgttcgac gagacgtacg agttcgcaga gcgcgtctca
     1381 cgctctctcg aggagcgcca tccgggtctt gtcacgaccg aatggctgaa gcgcaagcgg
     1441 agcggcgtgc tcgtcgacca ccggcagaac ggctggggca agacgatcgc ctcggtctac
     1501 tcggtgcgtc cgcgtccggg cgcgccggtc tcgacgccgc tgcgctggga ggagctgacc
     1561 gaggaggtga cgccgcggca gttcgggatg caggaagcgc tcgcacgggt cgagcgcgag
     1621 ggtgacgtct acgagccggt gctgcgcggc cggcaggcgc tcggccctgc cctccggaag
     1681 ctgcggtgaa cgcacgtccg cgcggagctg ccaggaaccg cacgatg
//
LOCUS       DASZOB010000382         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_159653, whole genome shotgun sequence.
ACCESSION   DASZOB010000382 DASZOB010000000
VERSION     DASZOB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159653"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VG709_06095"
     CDS             <1..252
                     /locus_tag="VG709_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC32684.1"
                     /translation="QWALSTYGLQRIVPDRIRGRVMSFDYGFVTLTMGASILVAGWAA
                     DRVDPRIVMAVLASVAVSYAVVWSALTGAIRRRETFELV"
     gene            complement(249..1040)
                     /locus_tag="VG709_06100"
     CDS             complement(249..1040)
                     /locus_tag="VG709_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32685.1"
                     /translation="MSPNTVLYALIFGAVGVMVFAVFYGDERGWGAGLTLGVSFGALI
                     VAAIVYAIYESARKRRRREAAPMAAASLGLTAESAGGRPLPAMPPFELMSRGMGRSAE
                     NVMSGDVGDDDVWLFDYTFYTESTTYNSTTGTTSTSRHDHYFSCVLAALPGDAPAMTI
                     GREGFFSKIARGLGFEDVELDDKDFDRRFKVKAADRDAAVAILDPVVRQWLMTSRDDV
                     QYELSGRYLLAYAKQRDLDELRALLDAAVGFREQLRGALTSSAGG"
     gene            1216..>2265
                     /locus_tag="VG709_06105"
     CDS             1216..>2265
                     /locus_tag="VG709_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32686.1"
                     /translation="MEPVNTLVTRRVRRGVSVLAGAALLIAAGAPPASAAPETDLVSK
                     TSGGEPANSWSSVGSISADGRFVAFESDATNLPGNDSWDVYVHDRRTGATELVSKTSG
                     GEPANGWSFSPSISASGRFVAFDSDATNLPGGADDAVLDVYVHDRWTGTTELVSRTSG
                     GDPANGGSGAPSISASGRFVAFASDARNLPGDDLFTEIYLHDRRTGRTRLVSKTSAGD
                     PMFGDSLNSSLSASGRFVAFESAFNFAYVHDWRTGTTKLVSRTSDGDRVNGSFPSISA
                     DGRFVAFASDARKLPGNDGVSDVYVRDRRTRTTSLVSRTSRGEPANADSYLPFISADG
                     RFVAFESDATNLPGND"
BASE COUNT          349 a          794 c          772 g          350 t
ORIGIN      
        1 cagtgggcgc tctcgacgta cgggctccag cggatcgtcc cggaccgcat ccgcggccgc
       61 gtgatgtcgt tcgactacgg gttcgtgacg ctcacgatgg gcgcctcgat cctcgtcgcg
      121 gggtgggcgg ccgaccgcgt cgatccgcgg atcgtgatgg ccgtcctggc ctcggtcgcc
      181 gtctcgtacg cggtggtgtg gtccgcgctg accggcgcga tccgtcgccg ggagacgttc
      241 gagctcgtct agccgccagc ggacgaggtg agcgcgccgc gcagctgctc gcggaacccg
      301 acggccgcat ccagcagcgc gcggagctcg tcgaggtcgc gctgcttcgc gtacgcgagg
      361 aggtaccggc ccgacagctc gtactgcacg tcgtcgcgcg acgtcatcag ccactgacgg
      421 acgacggggt cgaggatcgc gacggctgcg tcgcggtccg cggccttgac cttgaacctg
      481 cggtcgaagt ccttgtcgtc gagctcgacg tcctcgaacc cgagacctcg cgcgatcttc
      541 gagaagaacc cctcgcgccc gatcgtcatc gccggcgcgt cgcccggcag cgccgccagg
      601 acgcacgaga agtagtgatc gtggcgagac gtgctcgtcg tcccggtggt cgagttgtac
      661 gtggtgctct ccgtgtagaa cgtgtagtcg aagagccaca cgtcgtcgtc gccgacgtcc
      721 ccggacatca cgttctcagc ggaccgcccc atgccgcggc tcatcagctc gaagggcggc
      781 atcgcgggaa gcggtctccc gccggcggat tcggccgtga gccccagcga cgcggcggcc
      841 atcggtgccg cctcacgccg ccgtcgcttc cgcgcgctct cgtagatcgc gtagacgatc
      901 gcggcgacga taagggcccc gaacgagacg ccgagcgtca atcccgctcc ccatccccgc
      961 tcgtcgccgt agaagacggc gaagaccatc acgccgacgg cgccgaagat gagcgcgtag
     1021 aggacggtgt tcgggctcaa ggagccaatg ctaagcgacc gacgctatga ccaagagcgg
     1081 agcttgaccc cgctcgagcg gggaagtcag gaaagtagcg gacacgcgcc ccctgggcga
     1141 ggaccgtgac ttctcggcac ctcgggcccg aggagtacga tgctcgccat ggcgggacgg
     1201 tccgccggag cgcgcgtgga gccggtgaac accctggtga cgcggcgcgt ccgccggggg
     1261 gtttcggtgc tcgctggcgc ggcgctcctc atcgcggcag gggctccgcc cgcctccgcg
     1321 gcccccgaga ccgatctcgt gagcaagacc tcgggcggcg agccggcgaa ctcgtggagc
     1381 tccgtcggct cgatctctgc ggacggccgg ttcgtggcct tcgagtccga cgcgacaaac
     1441 ctccctggga acgacagctg ggacgtctat gtccacgacc ggcggaccgg ggccaccgag
     1501 ctcgtgagca agacctcggg cggcgagccg gcgaacgggt ggagcttcag tccctcgatc
     1561 tccgcctcag gccggttcgt cgccttcgac tccgacgcca caaacctccc cggcggcgcg
     1621 gacgacgccg tcctcgacgt ctacgtccac gaccggtgga ccgggaccac cgagctcgtg
     1681 agtaggacct cgggcggcga cccggcgaac ggggggagcg gcgctccctc gatctccgcc
     1741 tccggccggt ttgtggcctt cgcatccgac gccagaaacc tccccgggga cgacctcttc
     1801 accgagatct acctccacga ccggcggacc gggagaaccc ggctcgtgag caagacgtcg
     1861 gccggcgacc cgatgttcgg ggacagcctc aattcctcgc tctctgcctc cgggcggttc
     1921 gtggccttcg agtctgcctt caatttcgcc tatgtccacg actggcgaac cgggacgacc
     1981 aagcttgtga gcaggacctc tgacggggat cgggtgaacg gctcgttccc atcgatctcc
     2041 gcggatggcc ggtttgtggc ctttgcgtct gacgctagga agcttcccgg aaacgacggc
     2101 gtcagcgatg tctacgtccg agaccggcga acccggacga ccagcctcgt gagcaggacg
     2161 tcgcgcgggg aaccggcgaa cgcggacagc tacttgccct tcatctccgc ggacggccgg
     2221 ttcgtggcct tcgagtccga cgcgacaaac ctccctggga acgac
//
LOCUS       DASZOB010000383         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_175569, whole genome shotgun sequence.
ACCESSION   DASZOB010000383 DASZOB010000000
VERSION     DASZOB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175569"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VG709_06110"
     CDS             complement(<1..105)
                     /locus_tag="VG709_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWC32687.1"
                     /translation="MRVLVTGGAGFIGSHFVKRLAASRDDVVVLDKLTY"
     gene            complement(373..1215)
                     /gene="rfbD"
                     /locus_tag="VG709_06115"
     CDS             complement(373..1215)
                     /gene="rfbD"
                     /locus_tag="VG709_06115"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWC32688.1"
                     /translation="MGRSARPAPLADAGPAPLASRRVLLTGAGGQLGAALAEAFPDVD
                     ARTRAEFDVTRPVEVGYQPDLVLHAAAWTDVDAAEREAAAAAAVNVAGTRNVVALGAP
                     VVYFSSDYVFDGVRREPYVESDEPNPLSVYGRSKLLGERQVRRGWVVRTSWLFGWTGH
                     NFVRTMLRLGAERDEVRVVGDQRGCPTYVGDLAAAVRDVVGRQEGVWHLAADGDCTWA
                     DFAEAIFAETGTSCHVVRISTEEYGAAAPRPAYSVLRSERPDAPRLPHWRDGLRACLE
                     RLGA"
     gene            complement(1140..1619)
                     /locus_tag="VG709_06120"
     CDS             complement(1140..1619)
                     /locus_tag="VG709_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HWC32689.1"
                     /translation="MSAPTGGVQGVLRIPLRYHADERGWLLEVRRESALPKRTVQTNV
                     SFSRRGVIRGLHYHDRGQDDLFTCLSGTARVVVLDRGTGDVYCEDIGEENPVAVYVPG
                     RHAHGFEALTDVVFSYHVTAEYDPADPDERGIPWDDPRVRHLWQTQAPLLSPRDASS"
     gene            complement(1616..>1821)
                     /locus_tag="VG709_06125"
     CDS             complement(1616..>1821)
                     /locus_tag="VG709_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWC32690.1"
                     /translation="ADVFRMIPELEPSSRGELEITDVNNWYVAQGAMEYDVLEGFWGD
                     AGESIDAYYAVNDFVREHGANRP"
BASE COUNT          256 a          680 c          648 g          237 t
ORIGIN      
        1 gtaggtgagc ttgtcgagga cgacgacgtc gtcccgcgac gcggccagcc gcttcacgaa
       61 atggctgccg atgaaccccg ccccgccggt gactagcacc cgcacggcgc gatgctagcg
      121 gcgcgtcgta gccgcgcgac cggcttcccc gcgccggtgc cctcacggga ccgcgccgcg
      181 gtggccatgt tgggtgcctg gcacgagaca tggccttgcg cggcgcgccg cggatcgccc
      241 gctggggcgg gcgggcgcac cgccgcccgc ctcgctcgcg cagcgatgtc ccgccgggcc
      301 acgtcgggtg cctggcaccg gacatggaca gcctagccgt gccttgggcg acgagctatc
      361 cgggcgggca agctacgcgc cgagccgctc gaggcaggcg cggagaccgt cgcgccagtg
      421 cggcagacgc ggcgcgtccg ggcgctcgct tcggaggacc gagtacgccg gccgcggcgc
      481 ggcggcgccg tactcctccg tcgagatgcg aacgacgtgg cacgaggtgc ccgtctcggc
      541 gaagatcgcc tcggcgaagt cggcccacgt gcagtctccg tccgcggcga gatgccagac
      601 gccctcctgc cgcccgacga cgtcgcgcac cgccgccgcg agatccccga cgtacgtcgg
      661 gcagccgcgc tggtcgccga ccacgcgaac ctcgtcccgc tcggctccga ggcggagcat
      721 cgtccgcacg aagttgtgtc ccgtccagcc gaagagccac gacgtccgga cgacccagcc
      781 gcgccgaacc tggcgctcgc cgaggagctt cgagcggccg tacacagaca gcgggttcgg
      841 ttcgtccgac tccacgtacg gctcgcgcct gaccccatcg aacacgtagt cgctcgagaa
      901 gtagacgacc ggcgcgccga gcgcgaccac gttccgcgtg ccggcaacgt tcaccgccgc
      961 ggcggccgcg gcctcgcgct cggccgcgtc cacgtcggtc caggccgcgg cgtgcagcac
     1021 gaggtcgggc tggtaaccga cctcgacggg gcgcgtgacg tcgaactccg cgcgcgtccg
     1081 cgcgtcgacg tcgggaaacg cctccgcgag cgcggcgccg agctggccgc cggcgccggt
     1141 caggaggacg cgtcgcgagg cgagaggagc ggggcctgcg tctgccagag gtgccggacg
     1201 cgcggatcgt cccatgggat gccgcgctcg tcgggatcgg ccgggtcgta ctcggcggtc
     1261 acgtggtacg agaagacgac gtccgtcagg gcctcgaacc cgtgcgcgtg gcggccggga
     1321 acgtacaccg cgacggggtt ctcctcgccg atgtcctcgc agtacacgtc acccgtcccg
     1381 cggtcgagga cgacgacgcg cgccgtcccg gagagacagg tgaagaggtc gtcctggccc
     1441 cggtcgtggt agtggaggcc acggatcacg ccccggcggg agaacgaaac gttggtctgc
     1501 accgtccgct tcggaagcgc gctttcccgg cgcacctcga gcagccagcc gcgctcgtcg
     1561 gcgtggtagc gaagcgggat gcgcaggacg ccctgcacgc cgcccgtcgg cgcgctcacg
     1621 gcctgttcgc cccgtgctcg cgcacgaagt cgttgaccgc gtagtaggcg tcgatcgact
     1681 cgcccgcgtc gccccagaac ccctcgagca cgtcgtactc catcgccccc tgcgccacgt
     1741 accagttgtt cacgtccgtg atctcgagct ccccgcgcga cgaaggctcg agctccggga
     1801 tcatcctgaa cacgtcggcg t
//
LOCUS       DASZOB010000384         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78818, whole genome shotgun sequence.
ACCESSION   DASZOB010000384 DASZOB010000000
VERSION     DASZOB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78818"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1141)
                     /gene="rpsA"
                     /locus_tag="VG709_06130"
                     /pseudo
     CDS             complement(92..1141)
                     /gene="rpsA"
                     /locus_tag="VG709_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            1327..2316
                     /locus_tag="VG709_06135"
     CDS             1327..2316
                     /locus_tag="VG709_06135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWC32691.1"
                     /translation="MDEAFTVKRRVTFVVPGGVDADTGGNVYDRAAMSALATRGWDIV
                     VVETAEATDSDVVVVDSLAMPAGMPSTRAPVVALVHQLPSEAAGLEPSAGEVAVLRAA
                     SRVVTVSEWLAGRLRRLGAGSIGVVRPGRDRAWASEGPRADASTVLCVANAYPGKGLA
                     EAVEAFSNTGLPAITLRIAGDLGVDHDEASRVRAALERSAARVELLGVVPPPALSIAY
                     GEALVLIVASRYEGWPIAVAEAMASGVPVVGFDVPGVAELVRDGIDGVLAPVGDIDAL
                     SRATAAVCGDARLRARLGAAARARALGWPTWEESGERFAEEIERLAAQESGAP"
     gene            complement(2295..2681)
                     /locus_tag="VG709_06140"
     CDS             complement(2295..2681)
                     /locus_tag="VG709_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWC32692.1"
                     /translation="MTEGYEIGTAVRLRAFHVMPDMPPPEGERHAHDYRVEAIVRRRE
                     LDERGMVVDLDELRDALEEGAAHVHGRDLDRIRPESTDAVTVEVFARWFHGEVAERVR
                     RAGAEELEIRVWESPDDYGAYGAPLS"
     gene            complement(2678..3670)
                     /locus_tag="VG709_06145"
     CDS             complement(2678..3670)
                     /locus_tag="VG709_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HWC32693.1"
                     /translation="MRARAVVFTAPRSVEVRDVELDPPRDGDVVIRTTASGISGGTEL
                     LAYRGEIEPDLPLDERLGALGGTFTYPFRYGYSSVGIVERGARDVQEGSMAFAFYPHQ
                     DVAVVPADDAIAVDDVEPRIATMFPIVETGLQISLDAGDVSGRTVVVVGQGPIGLVSA
                     LLLARRGAEVIAVEPVPSRRRMASELGIRAHAPGAVGEAVTEATGGAGVPLVIEASGA
                     PTALADALSLAAHEGTVLVASWYGSKRVMLPLGGAFHRRRLTIRSTQVSTIPERLRGE
                     WTIERRRAEARALLSAHPLAPLATHAVPFDDAASAYEIIDRGENDAMHVALTYR"
     gene            complement(3667..>4064)
                     /locus_tag="VG709_06150"
     CDS             complement(3667..>4064)
                     /locus_tag="VG709_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWC32694.1"
                     /translation="VRVVAGTATALPLAARVADQLWCLGAVAHVDDLAALAGEAARVL
                     RTGGSIAITEAFSGGEAVPRFVRTAPRPWRPVTVAELVDALRAAGFVGVRHDPWPADV
                     SESPPSDSLLAADLREGRLRPALVTAQRA"
BASE COUNT          567 a         1428 c         1458 g          611 t
ORIGIN      
        1 ccgcaaaggc cgcgcccatc gcggagctcg gcgcggcgac ttcgtcgccc tgcgtggtcc
       61 cggcgctgaa cgccggctgc tcctcgatct cgggctcgct gccgtagccg gccttgacgg
      121 cctgcttcag cgacagcgag atgcgccggc gctggtcgtc gatgtcgatg atcttgacgg
      181 tgatccggtc gttgaccttc acgacctgct cggggacctc gacgtgcgcc tcggcgagct
      241 cggagatgtg gatcaacccc tccatgccct cctcgacctt cacgaaggcg ccgaacggca
      301 cgagcttggt gacgttgccc tcgatgaact caccgacgta gtgctggcgg gcgaactgac
      361 gccacgggtc ttcctgcgtc gccttcagcg acagcgacac ccgctcccgg tccatgtcga
      421 cgtcgaggac ctcgacgtcg acctcctggc cgacggcaac gacctcgctc gggtgctcga
      481 cgtggcgcca ggagagctcg gatacgtgga cgagaccgtc gacgccgccg agatccacga
      541 acgcaccgaa gttgacgatc gacgagactg accccttgcg cttctcgccc ttctgcagcg
      601 actcgaggaa gcgcttgcgt ccctccgact gcgactcctc gaggaatgca cgacgagaga
      661 gcacgacgtt gttgcggttg cggtcgagct cgatgatctt gcactcgagc tcctgcccga
      721 cgtacgggtg caggtcccgc acgcgccgaa gctcgacgag cgacgccgga aggaacccac
      781 ggagcccgat gtcgaggatg agcccgccct tgacaacctc gatgacgggg ccggtgatcg
      841 tcccgccctc gaccatgatc ttctcgatgc ggccccacgc gcgttcgtac tgcgcgcgct
      901 tcttggagag gatcagccgt ccttccttgt cctccttctg gaggacgagg gcctcgacct
      961 cgtcgccgac cttcacgacc tggtcggggt cgacgtcgtg acggatcgag agctccttgg
     1021 cggggatcac gccttcggac ttgtagccga tgtccaggag gacctcgtcg cggtcgatgc
     1081 gaacgatgcg tccggagacg aggtctccgt cgttgaagtc cttgagcgag gcctccatgg
     1141 cctcgacgag ctgctcgggc gtcaggtcgt cctgcggctc ggttgcggtc gcttccgtct
     1201 cgatgttccc tcagctcctt ccgccgggtg ctccgagcgt gacgtgacgc aggcgcggcc
     1261 tgggaacacg tggcgacgga agcctccggg ctttggaatc ttaggcgacc ataacgacga
     1321 ccgtcaatgg atgaagcgtt cactgtgaag cgtcgcgtca cgttcgtcgt gccgggcggc
     1381 gtcgatgccg acaccggcgg caacgtctac gaccgggccg cgatgagcgc gctcgcgacg
     1441 cgcggctggg acatcgtcgt cgtcgagacc gcggaagcga ccgactccga cgtggtcgtc
     1501 gtcgacagcc tcgcgatgcc ggccggcatg ccgtcgacgc gagcgccggt cgtcgcgctc
     1561 gtgcatcagc tcccgtccga ggcggcgggt ctagagccgt cggctggaga ggtggcagtc
     1621 ctgcgggcgg cctctcgagt cgtcacggtc agcgaatggc tcgcggggcg cctgcgccgg
     1681 ctgggcgcgg ggtcgatcgg cgtcgtgcgg cccgggcgcg accgcgcgtg ggcgagcgag
     1741 ggtccccgag ccgacgcatc gaccgtcctg tgtgtcgcca acgcgtatcc gggcaagggg
     1801 ctggccgagg ccgtggaggc gttctcgaac accgggcttc ctgccatcac gctgcgcatc
     1861 gccggcgatc tgggcgtcga ccacgacgag gcgtcgcggg tccgcgccgc gctcgagcgg
     1921 agcgccgcac gggtcgagct cctcggcgtc gtgccgccgc cggcgctttc gatcgcgtac
     1981 ggagaggcgc tggtgctgat cgtcgcctcg aggtacgagg gatggccgat cgccgtggcc
     2041 gaggcgatgg cgagcggcgt ccccgtcgtc gggttcgacg tccccggcgt cgccgagctc
     2101 gtgcgcgacg gcatcgacgg tgtgctggca cccgtgggcg acatcgatgc gctgtcgcgg
     2161 gcgaccgctg ccgtgtgcgg ggacgcacgg ctccgcgcca ggctcggcgc ggcggcgcga
     2221 gcgcgtgcgc tcggctggcc gacatgggag gagagcggcg agcgcttcgc cgaggagatc
     2281 gagcgtctcg ccgctcagga gagcggcgcg ccgtaggccc cgtagtcgtc cggggactcc
     2341 cacacgcgga tctcgagctc ctcggcgccg gcgcggcgga cgcgctccgc gacctcgcca
     2401 tggaaccacc gcgcgaacac ctcgacggtg acggcgtcgg tggactccgg ccggatgcgg
     2461 tcgaggtcgc gtccatgcac gtgcgccgcc ccctcctcga gcgcgtctcg cagctcgtcg
     2521 agatcgacga ccattccgcg ttcgtcgagt tcgcgccgcc gcacgatcgc ttcgacgcgg
     2581 tagtcgtgcg cgtggcgctc gccttcgggc ggcggcatgt ccggcatgac gtggaaggca
     2641 cgcagccgga cggcggtccc gatctcgtag ccctcggtca tcggtacgtg agcgcgacgt
     2701 gcatcgcgtc gttctcccca cggtcgatga tctcgtacgc ggacgcggcg tcatcgaagg
     2761 gaaccgcgtg cgtcgcgagc ggcgcgagcg ggtgggccga tagcagcgcg cgcgcctcgg
     2821 cgcgccgccg ctcgatcgtc cattcgccgc ggagccgctc cgggatcgtc gagacctgcg
     2881 tgctccgtat cgtcagtctg cgtcggtgga acgcgccgcc gagcggaagc atgacccgct
     2941 tcgatccgta ccacgacgcg acgagcaccg tgccctcgtg cgccgcgagc gacagggcgt
     3001 cggcgagcgc cgtcggcgcg ccgctggcct cgatcacgag cgggacgccc gcgccccccg
     3061 tcgcttcggt cacggcctcg ccgacggcac cgggggcatg agcacggatg ccgagctccg
     3121 atgccatgcg gcggcgtgac ggaacgggct cgacggcgat cacttccgcg ccgcgccgag
     3181 cgagcagcaa ggcggagacg aggccgatcg gtccctgccc gacgacgacc acggtgcgtc
     3241 cgctcacgtc gccggcgtcg agcgagatct ggagcccggt ctcgacgatc gggaacatcg
     3301 tcgcgatgcg cggctcgacg tcgtcgacgg cgatagcgtc gtcggccggg acgacggcca
     3361 cgtcctggtg cgggtagaag gcgaaggcca tgctgccttc ctggacgtcc cgggcgccac
     3421 gctcgacgat cccgacggac gagtacccgt agcggaacgg atacgtgaac gtgcccccga
     3481 gcgcgccgag acgctcgtcg agcgggaggt ccggctcgat ctccccgcgg tatgccagga
     3541 gctcggtgcc gccgctgatt cccgatgccg tcgtgcggat gaccacgtct ccgtcccgcg
     3601 gcgggtcgag ctccacgtcg cgcacctcga ccgaccgagg agcggtgaag acgaccgcgc
     3661 gtgccctcac gcccgctgcg cggtgacgag agcaggacgg agacggcctt cgcggaggtc
     3721 cgccgcgagc agcgagtcgg acggcggcga ctcgctcacg tctgccggcc acggatcgtg
     3781 tcgcacgccg acgaagccgg cggcccgcaa cgcgtcgacg agctcggcga ccgtcaccgg
     3841 gcgccagggc cgcggagccg tgcgcacgaa gcgcggtacc gcttcgcctc ccgagaatgc
     3901 ctcggtgatg gcgatcgaac cgccggtcct gagcacgcgc gccgcctcgc cggcgagcgc
     3961 cgcgaggtcg tcgacgtgag cgacggctcc gaggcaccac agctggtcgg cgacgcgcgc
     4021 agcgagcggg agcgccgtgg cggtcccggc gacgacccgt accc
//
LOCUS       DASZOB010000385         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_58738, whole genome shotgun sequence.
ACCESSION   DASZOB010000385 DASZOB010000000
VERSION     DASZOB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58738"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VG709_06155"
     CDS             <1..270
                     /locus_tag="VG709_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32695.1"
                     /translation="SLHNAGTEPALVSVSLLGRAGRSAPGALADLAIPPGRTVVAGLT
                     AAAEEGPVTAVVEASRGEVVAAQTGVSDRGYAVVTGVPWASVSTG"
     gene            310..1383
                     /locus_tag="VG709_06160"
     CDS             310..1383
                     /locus_tag="VG709_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HWC32696.1"
                     /translation="MARHRRHARRLSGRIGGVRIAVGVGVALALVLGGTAYGAYRYDG
                     AAAGRLLPGVKIAGVDVGEMTRAEAIAAVRRAASGTLDRRITVRADGRRWRVTPAELG
                     TKAVVAPVVDRALSLGDRYSWASRVFYRIANRDVGASYDLTYRQNDDRIERFVGGIAR
                     QVEVDPRNAAVSFEDGELVLSRPREGQELLVNRSRKALARAMLGDRESVRFGLRSLEP
                     EVTDDDLGHTIVVRLSELKLYLYDGAELVKTYPVAAGTSEYPTPQGTWTIWDKRENPT
                     WVNPAPDGWGAEMPAVIPGGPGNPLGTRALYLDAPGIRIHGTSDTGSIGTFASHGCVR
                     MRREHVEELYEIVPIGTQVIVTQ"
     gene            1593..2579
                     /locus_tag="VG709_06165"
     CDS             1593..2579
                     /locus_tag="VG709_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32697.1"
                     /translation="MSSHGVPREGLTGQAAGDEPRPVRVLYLCGSQRSGTHILSWILA
                     GLEGVGVAGEVKRLWVRAARGPHGCTCGKSIGDCEVWSHVVSADGAFDGLDAPSIVRL
                     RDAALPPGRSWRTTRRLLASDVPLDPSTPQGRYGRLLADFYRAYASASGSSVVIDSSK
                     DPGQAALLRRVPGVSMVPVQVVRDPRAVLDSHRRRRSSRNRPSVRDATRVAATWTAIQ
                     LACESIRRARGSHGFLIRYEDWVVRPREALAPLCVALGGKPEDLPVDDRGVASLPPVH
                     PHGRRRRYMQGTMELTPDERWKRELDAADRAVVASMTLPLLLRYGYPVRVRS"
     gene            2617..>2918
                     /gene="manB"
                     /locus_tag="VG709_06170"
     CDS             2617..>2918
                     /gene="manB"
                     /locus_tag="VG709_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840776.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWC32698.1"
                     /translation="MGDLVSIFKAYDIRGIYPEQLDEEAARRIGAAFAAFADADEIVV
                     GRDCRPSSPALVEAFVDGATASGAGALDIGLATTDMLYYASGRLGLPGAMFTASHNP"
BASE COUNT          386 a         1045 c         1065 g          422 t
ORIGIN      
        1 tcgctccaca acgcgggaac cgaaccggct ctggtctcgg tgtcgctgct cggccgcgcc
       61 ggccgcagcg cccccggcgc gctcgccgat ctggcgattc cccccggccg caccgtggtc
      121 gccgggctca ccgccgccgc cgaggagggc cccgtcaccg ccgtcgtcga agcctcgcga
      181 ggcgaggtcg tggccgccca gaccggcgtg tccgaccgcg gttacgccgt cgtgacggga
      241 gtgccctggg cgtccgtatc gaccggttaa cccattgacg gagcaagcca gcggtatatc
      301 atcgcgccga tggccaggca caggcggcac gcgcggcggc tctcgggccg catcgggggt
      361 gtacggatcg cggtcggcgt cggggtcgcg ctcgcgctcg tgctcggcgg caccgcgtat
      421 ggggcgtacc gctacgacgg cgccgccgcc gggcgcctcc ttcccggcgt caagatcgcg
      481 ggcgtcgacg tcggcgagat gacgcgagcc gaggcgatcg cggcggttcg ccgcgccgcc
      541 tcggggacgc tcgaccgccg gatcacggta cgggccgacg gacgtaggtg gcgcgtcacg
      601 ccggcggagc tcggcacgaa ggccgtggtg gcccccgtcg tcgaccgagc gctgtcccta
      661 ggggaccggt actcctgggc gtcccgcgtc ttctaccgga tcgcgaaccg agacgtcggc
      721 gcctcctacg acctcacgta tcgccagaac gacgaccgga tcgaacgctt cgtcgggggg
      781 atcgcgaggc aggtcgaggt cgacccgagg aacgcggcgg tgtcgttcga ggacggcgag
      841 ctggtcctga gccggccgcg cgagggccag gagctgctgg tcaaccgctc gaggaaggcg
      901 ctcgcccggg cgatgctggg cgatcgcgag agcgtgcggt tcgggctcag gtcactcgag
      961 ccggaggtga ccgacgacga cctcggccac acgatcgtcg tgcggctctc ggagctcaag
     1021 ctctatctct acgacggcgc ggagctcgtc aagacgtatc cggtcgccgc cgggacctcc
     1081 gagtatccga cgcctcaggg gacatggacg atctgggaca agcgcgagaa cccgacgtgg
     1141 gtcaaccccg cgcccgacgg gtggggtgcc gagatgccgg ccgtcatccc cggtggcccc
     1201 ggcaacccgc tcgggacgcg ggccctctac ctcgacgccc ccgggatccg gatccacggg
     1261 acgtcggaca ccgggtcgat cgggacgttc gcctcgcacg gctgcgtccg gatgcggagg
     1321 gagcacgtcg aggagctcta cgagatcgtc cccatcggca cccaggtcat cgtcacccag
     1381 tagcgaccgc cctccgcgag gaagttcgcc aagaacgcgc tacggggttg cgtgcgccga
     1441 tcgccgggtg tagcctgatg gagtactccg gagagatcgg aaggggcgac ccggacggtc
     1501 acttcggagt acgcttttct acgggccgca ttcgaggctc ccccgctctc tctcaccgac
     1561 gcggaaccgc cgttcggtac agtcgtgtcg cgatgtcatc tcacggggtc ccgcgagagg
     1621 ggctcacagg acaggccgcc ggcgatgagc ctcgtcccgt ccgtgtcctc tacctctgcg
     1681 gctcccagcg aagtgggacc cacatccttt cgtggatcct ggcggggctc gagggcgtcg
     1741 gggtcgccgg cgaggttaag cgcctctggg tccgcgccgc acgggggccg cacggctgca
     1801 cctgcggcaa gagcatcggc gactgcgagg tctggtcgca cgtcgtctcg gcggacggcg
     1861 cgttcgacgg gctcgacgcg ccctcgatcg tccggctgcg ggacgccgcg cttccgcccg
     1921 gacgctcgtg gcggacgacg cgccgcctgc tcgcctcgga tgtgccgcta gacccctcaa
     1981 ctccccaggg gcggtacggg cgactgctgg cggacttcta tcgggcgtac gcgtccgctt
     2041 ccgggtcgag cgtcgtcatc gactcctcga aggacccggg tcaggccgcg ctcctgcgcc
     2101 gcgtcccggg cgtctccatg gtccccgtcc aggtggtccg cgaccctcgg gccgtcctgg
     2161 actcgcaccg gcggcggcgc tcctcccgga accggccgag cgtccgcgac gcgacgcggg
     2221 tggcggcgac gtggacggcg atccagctcg cctgcgagtc gatccgccgc gctcgcggga
     2281 gccatgggtt cctcatccgg tacgaggact gggtggtgcg gccgcgcgag gcgctcgcgc
     2341 cgctgtgcgt cgcgttaggg gggaagcccg aggacctccc ggttgacgac cgcggcgtgg
     2401 cctcgctgcc gccggtgcat ccgcacggtc ggcgccggcg gtacatgcag gggacgatgg
     2461 agctcacgcc cgacgagcgc tggaagcggg agctggacgc ggccgatcgc gccgtcgtgg
     2521 cgtcgatgac gctgccgctg ctgctgcggt acggctaccc cgtccgcgtc cggagctgac
     2581 ggggggcgac gagggcgccc gggtagaatc cgccgcgtgg gcgacctcgt ctcgatcttc
     2641 aaggcctacg acatccgcgg catctatccc gagcagctgg acgaggaggc cgccagacgg
     2701 atcggcgccg cgttcgccgc attcgccgac gccgacgaga tcgtcgtcgg gcgcgactgc
     2761 cggccgtcct cacccgcgct cgtggaggcc ttcgtcgacg gggcgacggc ctcgggcgcc
     2821 ggcgccctcg acatcgggct cgccacgacc gacatgctct attacgcctc gggacggctc
     2881 gggcttccgg gcgcgatgtt cacggccagt cacaaccc
//
LOCUS       DASZOB010000386         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_44385, whole genome shotgun sequence.
ACCESSION   DASZOB010000386 DASZOB010000000
VERSION     DASZOB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44385"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VG709_06175"
     CDS             <1..309
                     /locus_tag="VG709_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32699.1"
                     /translation="IPPPYVPKNEIEDLPLEVRGFEPVDTLTDHSEHGLGLAERAAVD
                     GREWLKRGGWLLVEVSPDRARGIRSLLSRTGYRDVRRTAGWPNVTRVVVGRSERRRSP
                     "
     gene            750..1487
                     /locus_tag="VG709_06180"
     CDS             750..1487
                     /locus_tag="VG709_06180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWC32700.1"
                     /translation="MHRERVLLVGGQRALVMQLAHPKVAAGVARNSDFPARALERLRR
                     TLDLTLATVFGTPAEAEEAAASIRAVHGRVVGEAGAVGRYDASDPELLVWVNATLVDT
                     TMAVYERFVGPLPDDVWRRYYDESLDAAVQFGIPQGAVPPDLHSFRAYMREMLAGRSL
                     RATDDGRRLVRDVLKPPLPFPWRPPTAAVRLVTLALIPDEVRRLFGLHAGPGSRAALA
                     AASRASRAVLPLLPPAVREFSRARSAA"
BASE COUNT          203 a          561 c          587 g          236 t
ORIGIN      
        1 atccccccgc cgtacgtccc gaagaacgag atcgaggacc tcccgctcga ggtccgaggg
       61 ttcgaaccgg tcgacacgct gaccgatcac tccgagcacg ggctcgggct cgccgaacgc
      121 gccgccgtcg acggccgtga gtggctgaag cggggcgggt ggctcctcgt cgaggtgagc
      181 cccgaccggg cacgtgggat caggtcgctg ttgtcgcgga ccggctaccg tgacgttcgc
      241 agaaccgccg gctggccgaa cgtcacccgc gtcgtcgtcg ggaggtcgga acgacggcgg
      301 tcgccgtagc gtcgaaccag cggtgaggcg atacgaggtc ctcttcagtg gggtctgagg
      361 atcgattctt gcgcctcgca cgcgatccga ggagcagagg ttccgccggc tcgcggcatc
      421 ggcgcgcccg cgggagcggt gccggcggcg ccgctggagg ttgtcgggaa cgcgcgtggc
      481 actctcgtgc ccatgcttgt cgctggcgcc gtcggcgtgt tgctcggcgt gctcctcggc
      541 gcgacggtcg tcgcgagagc ggcacggcgc ggcgagcgca cgaacgggta gagggaaccg
      601 tcagcgggta cgggcaggac cctgatcagg acgcctccgg gctgaaggcc gctcgggcgt
      661 aacgaggggt tctgcgccgg acgacgccgt tcctgccacc ggcgcgcgcg ggtacgctag
      721 cggcgatgca tgctccgacg atggccgtgg tgcatcgaga acgcgtcttg ctggtcggag
      781 ggcagcgggc gctcgtgatg cagctcgccc atccgaaggt cgccgcgggc gtcgcgcgca
      841 acagcgactt cccggcgcgc gcgctggaac ggctccggcg gacgctcgat ctcacgctcg
      901 cgacggtgtt cggcaccccg gccgaggccg aagaggcagc cgcgagcatc cgtgcggtcc
      961 acggccgcgt cgtcggcgag gcgggcgcgg tcggacgcta cgacgcgagc gaccccgagc
     1021 tcctcgtctg ggtgaacgcc acgctcgtcg acacgacgat ggccgtgtat gagcgcttcg
     1081 tcggaccgct gccggacgac gtgtggcggc ggtactacga cgagtcgctc gacgccgcgg
     1141 tgcagtttgg gatccctcag ggcgccgtcc cgccggatct ccactccttc cgcgcgtaca
     1201 tgcgcgagat gctcgccggc cgatcgctgc gcgcgaccga cgacggccgc cgcctcgtcc
     1261 gcgacgtcct caagccgccg ctgccgttcc cgtggcgtcc accgaccgcg gccgtgaggc
     1321 tcgtcacgct tgccttgatc cccgacgagg tccgccggtt gttcgggctg cacgccgggc
     1381 cgggctcgcg cgcggcgctc gccgccgcct cgcgtgcgtc gcgagccgtc ctcccgttgc
     1441 tcccgccagc cgtgcgcgag ttctcgcgcg ccaggagcgc cgcgtgatcg acttcacgat
     1501 cccgccggag ctcgaccacg tgcggtctcg cgtcgccgcg ttcgtgcgcg atgaggtgct
     1561 tccggcggag cgagatgcaa ccgaagc
//
LOCUS       DASZOB010000387         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_44388, whole genome shotgun sequence.
ACCESSION   DASZOB010000387 DASZOB010000000
VERSION     DASZOB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44388"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VG709_06185"
     CDS             <1..447
                     /locus_tag="VG709_06185"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWC32701.1"
                     /translation="VLLFYSFGSEVDTAEIASRLHDRGVRLLLPYLDETGIEAAEVLP
                     DDDLTPTDYGPKEPTRRTAVDPGEVDVVVAPGLAFDRRGGRLGYGGGHYDRYLARLGA
                     RATRIGIAFSMQLLEEIPTDAGDERLDVVVTDEETVRAGPGGTPHL"
     gene            458..748
                     /locus_tag="VG709_06190"
     CDS             458..748
                     /locus_tag="VG709_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWC32702.1"
                     /translation="MPTYEYACNACGAHVEVVQRFDDEPLRTCTRCGGALRKVFHPAG
                     IVFRGSGFYATDSRTAPKGGGDGAKDTAKKEPSKTTDSSPSGTGGEKKEKSA"
     gene            760..1545
                     /locus_tag="VG709_06195"
     CDS             760..1545
                     /locus_tag="VG709_06195"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWC32703.1"
                     /translation="MGVFGGSGLYEFLDDVEEVELDTPYGKPSARLAVGEIEGRHVAF
                     LPRHGRRHQFPPHEVPYRANVWAMKELGVERIVGPCAAGSLQPHVKVGHFVVCDQLVD
                     WTRGRSETFYDGPETTHISFADPYCPTMREVAIKQARDQGLDLHERGTVLVVEGPRFS
                     TRSESAFYQRQGWEVINMTQSPEAVLARELELCYLNISLITDYDVGVEGEDMEPVSHE
                     MVIKVFQENTARLRTLLTRIVPALPRERDCPCATALEGARFEP"
     gene            1664..2284
                     /gene="cpaB"
                     /locus_tag="VG709_06200"
     CDS             1664..2284
                     /gene="cpaB"
                     /locus_tag="VG709_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWC32704.1"
                     /translation="MALSIVLGAVTTLALRDHLERVEARAAAPGPVARIVVAAADLER
                     GATLTAYDVAIRELPEAHAPVAAIASVDEVVGRVLASDVLAGEPLTETRLAPPGGPVA
                     ALVPEALRALPVTVALPRGTLVPGDRVDLLATYASGQPHTETVLAGVEVIRVVEAPGT
                     ERTPAATLLLLVTPEGSERVAFAKAFADLSVAVAPAAAAAAAWASP"
     gene            2325..3158
                     /locus_tag="VG709_06205"
     CDS             2325..3158
                     /locus_tag="VG709_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWC32705.1"
                     /translation="MLRAAVLGAVQGLTEFIPISSSAHLVLVPFLLGWAVPGLAFDVA
                     VHIGTAVAVVVYFWRDIGRILLGATRTVLGRGDAGDRTFARLLLLLALASIPAAIAGF
                     FLENTFESLFTTSGDVRRIGALAVGLFLLVTAALILVAEWAYARRERATSPRDVGTAD
                     AVVVGLLQATAIAPGISRSGATISGGMLRGLTREAAARFSFLLSLPAIIGAAIISLPD
                     LPANADLGPMLVGTGTSAVTGFAAIAFLLRYLRTRTMRPFAIYCLLLAAVAIGYWFQV
                     K"
     gene            complement(3160..>3671)
                     /locus_tag="VG709_06210"
     CDS             complement(3160..>3671)
                     /locus_tag="VG709_06210"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWC32706.1"
                     /translation="LPDGPASSPFDDEGVPTGRTPLIERGTLRAFLHNTQTAVRDGAS
                     STGNASRPGYRGVPGVAPTNLLLEPGGETAEALLGRAGTAVYVQDVTGVHSGANPVSG
                     EFSVGATGLRVDGGALAAPLREMTIASTLPDVLKAIEAVASDVRFFPGGGAIGTGTVL
                     VGEMTVAGA"
BASE COUNT          486 a         1352 c         1287 g          546 t
ORIGIN      
        1 gtcctgctct tctactcgtt cggatccgag gtcgacaccg ccgagatcgc gtcgcggctc
       61 cacgaccgcg gggtcaggct cctgctgccg tacctcgacg agactggcat cgaggccgcc
      121 gaggtactcc cggacgacga cctcacaccg accgactacg ggccgaagga accgacccgg
      181 cggacggcgg tggatcccgg cgaggtggac gtcgtcgtgg cgccgggtct ggcgttcgat
      241 cggcggggcg gccgcctcgg ctacggcggc gggcactacg accggtacct cgcgcggctc
      301 ggagcccgcg cgacgcggat cgggatcgcc ttctcgatgc agctcctcga ggagatcccg
      361 accgacgctg gggacgagcg gctcgacgtc gtcgtgaccg acgaggagac ggtccgcgcc
      421 gggcccgggg gaacgccaca cctataatcg tggcgcgatg cccacctacg agtacgcgtg
      481 caacgcctgt ggcgcgcacg tcgaggtcgt ccagcgcttc gacgacgagc cgcttcggac
      541 atgtacgaga tgcggcggcg cgcttcggaa ggtcttccat cccgccggaa tcgtcttccg
      601 cgggtccggc ttctacgcga ccgactctcg aaccgccccg aagggcggcg gagacggcgc
      661 gaaggacacc gcgaagaagg agccgtcgaa gacgaccgac tcgtcgccgt ccggcaccgg
      721 cggcgagaag aaggagaagt cggcctgacc agcgccgaga tcggcgtctt cgggggctcg
      781 gggctatacg agttcctcga cgacgtcgag gaggtcgagc tcgacacccc gtacgggaag
      841 ccgtcggcgc ggctcgccgt cggcgagatc gagggcaggc acgtcgcgtt ccttccgcgt
      901 cacgggcgac gtcaccagtt cccgccgcac gaggtgccgt accgcgcgaa cgtctgggcg
      961 atgaaggagc tcggcgtcga gcggatcgtc ggcccgtgcg ccgcgggctc gctccagccg
     1021 cacgtgaagg ttggtcactt cgtcgtgtgc gaccagctcg tcgactggac ccgcggacgg
     1081 agtgagacgt tctacgacgg gcccgagacc acgcacatct cgttcgccga cccctactgc
     1141 ccgacgatgc gcgaggtcgc gatcaagcag gcgcgcgacc aggggctcga cctccacgag
     1201 cggggcacgg tgctcgtggt cgaggggccc cggttctcga cgcgctccga gtccgcgttc
     1261 taccagcggc aggggtggga ggtcatcaac atgacgcaga gcccggaggc cgtgctcgcg
     1321 cgggagctcg agctctgcta cctcaacatc tcgctgatca ccgactacga cgtcggcgtc
     1381 gagggcgagg acatggagcc cgtcagccac gagatggtga tcaaggtgtt ccaggagaac
     1441 accgcgcgac tccggacgct gctaacccgc atcgtcccgg cgctcccgcg cgagcgagat
     1501 tgcccgtgcg cgacggcgct cgagggcgcc cgcttcgaac cgtagggatg tcaccccccg
     1561 gcgctaccct gcgccggggc cgaagagcgg cctccgaaga ccacctcctc ccccggaagg
     1621 gatggccatg cgtcgtcttc gcccgcgagg gtcccggctg ctcgtggcgc tctcgatcgt
     1681 tctcggcgcc gtcacgacgc tcgcgctccg cgatcacctc gagcgcgtcg aggcgcgagc
     1741 cgccgcgccg gggcccgtcg cgcggatcgt cgtcgcggcg gccgacctcg agcgcggagc
     1801 caccctgacc gcgtacgacg ttgcgatccg cgagctcccc gaggcgcatg cgcccgtcgc
     1861 ggcgatcgca tcggtcgacg aggtcgtcgg tcgcgtgctc gcgtccgacg tgctcgcggg
     1921 ggagccgctc accgagacgc ggctggctcc tcccggcggg ccggtcgccg cgctcgtccc
     1981 cgaggcgctc cgcgcgctgc ccgtcaccgt cgcgttgccg cggggcacgc tcgtccccgg
     2041 agaccgcgtc gacctcctcg ctacctacgc gtccgggcag ccgcacaccg agacggtgct
     2101 cgcgggcgtc gaggtgatcc gggtcgtcga ggcgccgggc acagagcgca cccccgccgc
     2161 gacgctgctg ctcctcgtga cgcccgaagg atccgaacgc gtcgcgttcg ccaaggcgtt
     2221 cgccgacctc tcggtagccg tcgcccccgc ggcggcggcg gcggcagcct gggcgtcacc
     2281 gtagagcggt cgccgacacc tccgacgccg ctacactcgc cggcatgctc cgcgccgcgg
     2341 ttctcggcgc cgtccagggc ctcaccgagt tcatcccgat ctcgtcctcc gcgcacctcg
     2401 tcctggtgcc tttcctcctc ggctgggcgg tcccgggtct cgcgttcgac gtggcagtgc
     2461 acatcggcac ggccgtcgcc gtcgtcgtgt acttctggcg cgacatcggc cggatcctcc
     2521 ttggggcgac gcggacggtt ctcggacgcg gcgacgctgg tgaccggacg ttcgcgcgcc
     2581 tgctgctcct cctcgcactc gcgtcgatcc ccgcggcgat cgccgggttc ttcctcgaga
     2641 acacgttcga gtcgctgttc acgacgtccg gcgacgtgcg caggatcggc gcgctcgccg
     2701 tcgggctgtt cctcctcgtg acggccgcgc tgatcctcgt cgccgaatgg gcgtacgcac
     2761 gccgcgagcg cgcgacctcg ccgcgcgacg tcgggacggc cgacgcggtc gtcgtggggc
     2821 tgttgcaggc gaccgcgatc gcgccgggga tctcgcggtc gggagcgacg atcagcggcg
     2881 ggatgctccg gggactcacc cgagaggccg ctgcgcgttt ctcgtttctg ctgagcctgc
     2941 ccgcgatcat cggcgccgcg atcatctcgc tgccggacct tcccgccaac gccgacctcg
     3001 gcccgatgct cgtcgggacg ggcacatcgg cggtgaccgg cttcgccgcg atcgcgttcc
     3061 tgctgcgcta cctgcgcacg cgcacgatgc gcccttttgc gatctattgc ctgctgctcg
     3121 ccgcggtggc gatcggctat tggttccagg tgaagtagct caggctccgg cgacggtcat
     3181 ctcgccgacg aggacggtcc ccgtaccgat cgctccccct ccgggaaaga accgcacgtc
     3241 cgacgcgacc gcctcgatcg ccttcagcac gtcggggagc gtgctagcga tcgtcatctc
     3301 ccggagcggc gctgcgagcg cgccgccgtc cactcggagg cccgtcgcgc cgaccgagaa
     3361 ctcgccgctc acggggttcg cgccggagtg cacgccggtg acgtcctgca cgtagacggc
     3421 ggtccccgcc cgacccagca gcgcctcggc cgtctcgccg cccggctcga ggaggaggtt
     3481 cgtgggggcg acgcccggta cgccgcggta gcccgggcga gacgcgttcc cggtcgacga
     3541 tgcgccgtcg cgcaccgcgg tctgcgtgtt gtggaggaac gcccgcagcg tcccgcgctc
     3601 gatgagaggc gttcgcccgg tcggcacgcc ctcgtcgtcg aacggcgagg acgccggccc
     3661 gtcgggaagc c
//
LOCUS       DASZOB010000388         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78875, whole genome shotgun sequence.
ACCESSION   DASZOB010000388 DASZOB010000000
VERSION     DASZOB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78875"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..241)
                     /locus_tag="VG709_06215"
     tRNA            complement(167..241)
                     /locus_tag="VG709_06215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Lys,seq:ttt)
     gene            complement(232..>1677)
                     /locus_tag="VG709_06220"
     CDS             complement(232..>1677)
                     /locus_tag="VG709_06220"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWC32707.1"
                     /translation="LQALEDLARRGADGCAVLVGFVDRTERGLHNAAALLRGGRVETR
                     YRKHHLPNYGVFDEKRYFVAGDGGRAVRVAGSDLGISICEDAWRPGAPWTEYAGGVRV
                     IANINGSPYHRKKTAERIDVCAARARETGAWIVYVNLVGGQDELVFDGGSMVVSPDGD
                     VVWHADAFEEDMLVVDVEDGADGQAPLPDPTREPWPEGAEEVYRALVLGLGDYVRKNG
                     FREVVVGLSGGIDSSLTATLAADAIGPEGVRALAMPSPYSSRDSLDDAADVAERLGIR
                     LDRVPIGPVFEAYLETVAPLFEGRDPDVAEENIQARIRGNLLMAVSNKFGSLVLATGN
                     KSELAVGYSTLYGDLAGGFAPLKDVPKTLVYELARWRNKQTEPAPIPDRVLDKPPSAE
                     LRPDQRDTDSLPPYDALDPILEAYVEEDRSPEEIVAAGADPRLAREVVAMVDRAEYKR
                     RQAPPGVKITPKAFGRDRRLPITNRYHRSGR"
BASE COUNT          238 a          619 c          575 g          245 t
ORIGIN      
        1 gggcgacccg agaagcgtgg tcatcgcgct cggccccgtg ccgccgaacc ccgatcgcgt
       61 cgagctccgc gcgaagatcg acgccccggg gtacggagaa acgcccgacg ctcgtgctcc
      121 agggcgacga tctccggtgg agggtcacgg agactcgcgg cggcggtggg gcgtgaggga
      181 ttcgaacccc cgacacatgg gttaaaagcc cactgctctg accaggctga gctaacgccc
      241 cgaccgatgg taccggttcg tgatcggcag cctccggtcg cggccgaacg ccttcggcgt
      301 gatcttcacc ccgggcggcg cctgtcgccg cttgtactcc gcccggtcga ccatcgcgac
      361 gacctcgcgt gcgaggcgcg gatccgcccc ggcggcgacg atctcctcgg gtgagcgatc
      421 ctcttcgacg tacgcctcga ggatcgggtc gagcgcgtcg tacggcggga gcgagtcggt
      481 gtcgcgctgg tccggacgaa gctcagccga cggaggcttg tcgaggacgc gatcggggat
      541 cggcgcgggc tccgtctgct tgttccgcca ccgcgcgagc tcgtacacga gggtcttcgg
      601 gacgtccttc aacggcgcga acccccctgc aaggtccccg tacagcgtcg agtagcccac
      661 ggcgagctcg ctcttgttgc cggtcgcgag cacgagagag ccgaacttgt tcgagaccgc
      721 catgagcagg ttgccgcgga tccgagcctg gatgttctcc tcggcgacgt cggggtcccg
      781 cccctcgaac agcggcgcga ccgtctccag gtacgcctcg aagaccggcc cgatcggcac
      841 gcggtcgagg cggatcccga gccgctcggc cacgtccgcg gcatcgtcga ggctgtcccg
      901 cgaggagtac ggcgagggca tcgcgagggc ccggacgccc tcgggcccga tcgcgtcggc
      961 ggccagcgtg gcggtgagcg acgaatcgat cccaccggag agtccgacga cgacctcgcg
     1021 gaagccgttc ttgcgaacgt agtcgccgag ccccaggacg agcgcccggt acacctcctc
     1081 ggcgccctcg ggccacggct cgcgcgtcgg gtcggggagc ggcgcctgcc cgtcggcgcc
     1141 gtcctcgacg tccaccacga gcatgtcttc ctcgaaggcg tccgcgtgcc agacgacgtc
     1201 accgtccggc gagacgacca tgctcccgcc gtcgaagacc agctcgtcct gtcctccgac
     1261 gaggttcacg tacacgatcc aggcccccgt ctcgcgggcg cgggccgcgc agacgtcgat
     1321 ccgttccgcg gtcttcttgc ggtggtacgg cgagccgttg atgttcgcga tgacgcggac
     1381 gccgccggcg tactcggtcc acggcgcacc cgggcgccac gcgtcctcgc agatcgagat
     1441 cccgaggtcg gatcccgcga cccgcacggc gcgtcccccg tcgcccgcga cgaagtagcg
     1501 cttctcgtcg aacacgccgt agttcggcag gtggtgcttg cggtaccggg tctcgacccg
     1561 accgcctcgc aggagcgccg ccgcgttgtg gagcccgcgc tccgtcctgt cgacgaaccc
     1621 gaccagcacc gcgcatccgt ccgccccccg acgcgcgagg tcctccagcg cctgcag
//
LOCUS       DASZOB010000389         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_169584, whole genome shotgun sequence.
ACCESSION   DASZOB010000389 DASZOB010000000
VERSION     DASZOB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169584"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VG709_06225"
     CDS             complement(<1..559)
                     /locus_tag="VG709_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWC32708.1"
                     /translation="MSAPQVEIQLIAAVTAAACALPGVFLVIRRMALLSDAISHSILL
                     GIVLAFFVVEDLTSPLLVVGAAVIGLVTVTCVELVGQTGLVREDAAIGLVFPALFSAG
                     VILISRFADSVHLDTDAVLLGELAFAPFDRLVVGGYDVGPRALYVMGAILVLNLVFIS
                     LFYKELELATFDPALALALGFAPGLI"
     gene            complement(600..1733)
                     /locus_tag="VG709_06230"
     CDS             complement(600..1733)
                     /locus_tag="VG709_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWC32709.1"
                     /translation="MPLDLFSDYTLRVVALGAATLGVVAGALGAFAVLRRQSLLGDAV
                     SHAALPGVVLAFALTGTKAPLALLVGAAFAGWAAALVVMAIVRTTRVKYDAALGLVLS
                     VFFGVGLVLLTDVQKRPDAAQAGLDRFLFGQAAGLLRDDVVTMALLGAPVLVALALLW
                     KEFKLLAFDPAFGASLGYPMRLLDVVLTALLVVAIVVGLQTVGVVLMSAMVVAPAAAA
                     RQWTDRLGVMVPLSAFFGAAAGVAGAVISSRAERMPAGPTIVLCVTVVVVVSLLAAPN
                     RGLLWRWAGERRRRRRLRVQTVLADLYALGAQHEGARGHPSTVVDVMEAGRGRSGRTL
                     SELEKRGWAERVGDDDWTLTAAGALEAERRFGPAEALADMERA"
     gene            complement(1738..>2021)
                     /locus_tag="VG709_06235"
     CDS             complement(1738..>2021)
                     /locus_tag="VG709_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HWC32710.1"
                     /translation="TERAVVELLRSLRSARKTVVAVHHDLQTVPEYFDWVTLLNVRRV
                     ASGPVGEVFTEENLRLCYGAPLAFFRPNGGPESRVPGAVTPVGSPGDAD"
BASE COUNT          353 a          699 c          769 g          200 t
ORIGIN      
        1 ggatgaggcc gggggcgaat ccgagcgcga gggcgagcgc cgggtcgaac gtcgcgagct
       61 cgagctcctt gtagaagagc gagatgaaga ccaggttcag gacgagaatc gcccccatga
      121 cgtagagcgc gcgcggaccc acgtcgtacc cgccgacgac caggcggtcg aacggcgcga
      181 acgcgagctc gccgagcagg acggcgtccg tgtcgaggtg gacgctgtcg gcgaagcgcg
      241 agatgaggat cacgccggcg ctaaagaggg cggggaagac gagaccgatc gccgcgtcct
      301 cacgcacgag gccggtctgc ccgacgagct cgacgcacgt cacggtcacg agcccgatga
      361 cggccgcgcc gaccacgagg agcggcgacg tcaggtcctc gacgacgaag aacgcgagga
      421 cgatcccgag caagatcgag tggctgatcg catcggagag aagcgccatg cggcgaatga
      481 cgaggaagac gccgggaagc gcgcacgcgg ccgccgtcac ggcggcgatg agctggatct
      541 cgacctgcgg cgcgctcacg tgccccccgc gcaccacgaa ggggtcgaca cggcgcttct
      601 cacgcgcgct ccatgtccgc gagcgcctcc gccggcccga agcgccgctc cgcctccagc
      661 gccccggctg ccgtcagcgt ccagtcgtcg tccccgacgc gctcggccca tccccgcttc
      721 tcgagctcgc tcagcgtccg ccccgagcga ccgcgtccgg cctccatgac gtcgacgacg
      781 gtcgacgggt ggcctcgggc gccttcgtgc tgggcgccga gcgcgtacag gtcggcgagg
      841 accgtctgca cgcggagacg ccggcgcctg cggcgctcgc cggcccaccg ccagaggagc
      901 ccgcggttcg gcgccgcgag cagggagacg accacgacga cggtgacgca gaggacgatc
      961 gtcgggcccg ccggcattcg ctcggcccgg ctcgagatga cggcgccggc cacgcccgcc
     1021 gcggcgccga agaacgccga gagtggcacc atgacgccaa ggcggtcggt ccactggcgc
     1081 gccgccgccg cgggcgcgac gaccatcgcg ctcatgagga cgacgccgac ggtctggagc
     1141 cccaccacga tcgcgacgac gagaagagcc gtgaggacga cgtcgaggag gcgcatcggg
     1201 tagccgaggc tcgcgccgaa ggcgggatcg aacgcgagca gcttgaactc cttccagagc
     1261 agcgcgagcg cgacgaggac gggcgcaccg aggagcgcca tcgtgacgac gtcgtcgcgc
     1321 aggagaccgg cggcctgccc gaacaggaag cggtcgagcc ccgcctgcgc cgcgtcagga
     1381 cgcttctgga cgtccgtgag gagcaccagg ccgacgccga agaagacgga caggacgaga
     1441 ccgagcgccg cgtcgtactt cacccgcgta gtgcggacga tcgccatgac gacgagcgcg
     1501 gcggcccagc cggcgaacgc ggcgccgacg agcagcgcga gcggggcctt cgtcccggtg
     1561 agcgcgaatg cgaggacgac ccccggcagc gcggcgtgcg agacggcgtc gccgaggagg
     1621 ctctggcggc gaaggacggc gaaggcgccg agcgccccgg cgacgacgcc gagcgtcgcg
     1681 gcaccgaggg cgacgacgcg gagcgtgtag tcggagaaga ggtcgagcgg caccgggcta
     1741 gtcggcgtcg cccggcgagc cgacgggcgt gacggcgccg ggaacgcggc tctcggggcc
     1801 accgttcggg cggaagaacg cgaggggcgc gccgtagcag aggcgcaggt tctcctcggt
     1861 gaacacctcg ccaaccgggc cgctcgcgac gcggcggacg ttcagaagcg tgacccagtc
     1921 gaagtactcc gggacggtct gtaggtcgtg atggaccgcg acgacggtct tccgtgcgga
     1981 gcgtagcgag cgcaggagct cgacgaccgc gcgctcggtc g
//
LOCUS       DASZOB010000390         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_118567, whole genome shotgun sequence.
ACCESSION   DASZOB010000390 DASZOB010000000
VERSION     DASZOB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118567"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1703
                     /locus_tag="VG709_06240"
     CDS             <1..>1703
                     /locus_tag="VG709_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWC32711.1"
                     /translation="TGKRFGLATINPMDDEGRFTDAAGPYARLFVKDADARIIEDLRA
                     SGKLWRSGEYEHTYPLCWRCSTPLLYWARNSWYIRTTARKRELLEANENVGWYPDHIK
                     HGRYGDWLSNNVDWSLSRERYWGTPLPVWRCAAGHTTVVGSRAELSALAGGDVGGGDP
                     HRPDIDEVVLGGPECGEESRRVPEVIDAWFDSGAMPFAQWGYPQHGERRFRERFPADF
                     IAEGIDQTRGWFYSLMAESVLLFGENAYRNVVCLGHIVDRDGRKMSKSLGNVLDPWAV
                     LDAQGADALRWYLLTSGSPWAARRVYPEAIEDVLRRFVLTVRNTHSFFVTYANIDRVD
                     AASIELPPQRRPPIDRWILSQLHDTVATAREGLERYDATGAGRRIDRFVDDLSNWYVR
                     RSRRRFWDPARPDDGAGRDDKDAAYATLFECLTTLSRLLAPFTPFLAEDLYGGLVAEV
                     ATDAMASVHLTDYPEPDPAALDPALDEAMATVRQIVSLGRSARKEAGVRVRQPLRRAV
                     VHVPGAPERLRPLLPLVAEELNVREVAFAISEGDVAGWRAKPAFRVLGPRLGPRVKDV
                     A"
BASE COUNT          235 a          618 c          606 g          244 t
ORIGIN      
        1 acaccggcaa gcggttcggc ctcgcgacca tcaacccgat ggacgacgag ggccgcttca
       61 ccgacgcggc cggcccgtac gcccgactct tcgtgaagga cgccgacgcg cggatcatcg
      121 aggacctccg agcgagcggg aagctctggc gctccggcga gtacgagcac acctatccgc
      181 tctgctggcg gtgctcgacg ccgctcctct actgggctcg gaactcctgg tacatccgca
      241 ccaccgcgcg caagcgcgag ctgctcgagg cgaacgagaa cgtgggctgg tacccggacc
      301 acatcaagca cggccgctac ggcgactggc tctcgaacaa cgtcgactgg tcgctgtcac
      361 gcgagcgcta ctggggtacg ccgctgccgg tgtggcgttg cgccgctggg cacacgaccg
      421 tcgtcgggtc gcgggcggag ctctccgcgc tcgccggagg cgacgtcggc gggggggacc
      481 cgcaccgtcc cgacatcgac gaggtggtcc tcggcggccc cgagtgcggc gaggagtccc
      541 ggcgcgtgcc ggaggtcatc gacgcgtggt tcgactccgg cgcgatgccg ttcgcccagt
      601 ggggctaccc gcagcacggc gagcgacggt tccgcgagcg gttcccggcg gacttcatcg
      661 ccgaggggat cgaccagacg cgggggtggt tctactcgct gatggccgag tcggtgctgc
      721 tgttcggtga gaacgcatac cgcaacgtcg tctgtctcgg ccacatcgtc gaccgcgacg
      781 gccggaagat gtcgaagtca ttgggcaacg tcctcgaccc atgggcggtg ctcgacgcgc
      841 agggcgccga cgcgctccgc tggtacctgc tcacgtccgg atcgccgtgg gcggctcggc
      901 gcgtgtaccc ggaggcgatc gaggacgtcc tccggcgctt cgtcctgacc gtccgcaaca
      961 cccactcgtt cttcgtgacg tacgcgaaca tcgatcgcgt cgacgcggcg agcatcgagc
     1021 tccccccgca gcggcgcccg ccgatcgacc gctggatcct ctcccagctg cacgacacgg
     1081 tcgcgaccgc gcgcgagggg ctggagcgct acgacgccac gggcgccggg cgccggatcg
     1141 accggttcgt cgacgacctg tcgaactggt acgtgcgacg atcgcgacgg cggttctggg
     1201 accccgcgcg ccccgacgac ggggcaggcc gcgacgacaa ggacgccgcc tacgcgaccc
     1261 tgttcgagtg tctgacgacg ctctcgcgcc tgctcgcgcc gttcacgccg ttcctggccg
     1321 aggacctcta cggggggctc gtggcggagg tcgcgacgga cgcgatggcg agcgtccacc
     1381 tcaccgacta ccccgagccc gatccggcgg cgctcgatcc ggcgctggac gaggcgatgg
     1441 ccaccgtccg ccagatcgtc tcgctcggac ggtcggcgcg gaaggaggcg ggcgtccgcg
     1501 tgcgccagcc gctgcggcgc gccgtcgtcc acgtccccgg cgctcccgaa cgcctccggc
     1561 cgctgctgcc gctcgtcgcc gaggagctca acgtccgcga ggtcgcgttc gccatctcgg
     1621 agggtgacgt ggccggctgg cgcgcgaagc cggcgttccg ggtgctcggt ccacggctcg
     1681 gcccgcgggt caaggacgtc gcc
//
LOCUS       DASZOB010000391         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_159779, whole genome shotgun sequence.
ACCESSION   DASZOB010000391 DASZOB010000000
VERSION     DASZOB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159779"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1882
                     /locus_tag="VG709_06245"
     CDS             <1..>1882
                     /locus_tag="VG709_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWC32712.1"
                     /translation="PNAIGGNGGAYAPTAAAPEAAARTDEVLLGAVAAAMALVKSHRM
                     HGHLAARLDPRGDDPPGDPALDPERLQPKLTPELQSRIPARLLRLYCPGETLAEALPN
                     LRKTYCETLAYQIEHISDHAERVWLRQAIESGRYTRPLEPDRKLALLTRLSEVEGFER
                     YLRRAFLGQKQFSIEGLDTMVPMLDEAIELAAATGARDVVLGMAHRGRLNVIAHVVGR
                     PYDVILREFEGERLIEAVTHDPEGGTGDVKYHHGSEGVRRTGSGEVRVRLASNPSHLE
                     AVDPVVVGETRAMQTDHASPEGSHDPTSALAVLIHGDAAFPGQGIVAETMNLQDLEGY
                     STGGTLHLIAHNQVGFTTDPGEGRSTRYSSDIAKGYDTPIVHVNADDPEASIAAIRLA
                     MAYRERFRRDVVVDLVGYRRFGHNEGDEPAYTQPLMYRAIEDHPTAREQYAASLVEEG
                     VVTGEEADELVRSVQERMREAHDALKASLEASGASARVERRPGRVGFEEPATAVPEER
                     LEVLNRELLAVPDGFTIHPKLAKQLERRLETLGPEGGIDWAQAESLALGSLVTEGVPV
                     RLTGHDTERGTFAHRHLVLHDAETGDKWTPIEHLAEAKAPFQVYNSPLSEAGCVGFEY
                     GYA"
BASE COUNT          305 a          698 c          649 g          230 t
ORIGIN      
        1 cgccgaacgc gatcggcggc aacgggggcg cgtacgcgcc caccgccgcg gcgcccgagg
       61 cggctgcccg gaccgacgag gtacttctcg gcgccgtcgc agccgccatg gcgctggtga
      121 agtcacaccg gatgcacggt catctcgccg cccgcctcga cccgcgcggc gacgatccgc
      181 cgggagatcc cgcgctcgac cccgagcggc tccagccgaa gctcacgccg gagctccaga
      241 gccggatccc ggcacggctc ctccgcctct actgccccgg cgagacgctc gccgaggcgc
      301 tgcccaatct gcggaagacg tactgcgaga cgcttgcgta ccagatcgag cacatctctg
      361 accacgccga gcgcgtatgg ctgcgccagg cgatcgagtc gggccggtat acccggccgc
      421 tcgagccgga ccggaagctg gcgctcctca cgcgactctc cgaggtcgag gggttcgagc
      481 gctatctccg ccgcgccttc ctggggcaga agcagttctc gatcgagggc ctcgacacga
      541 tggttccgat gctcgacgag gcgatcgagc tggcggccgc gacgggcgcg cgcgacgtcg
      601 tgctcggcat ggcgcaccgc ggccgcctga acgtgatcgc ccacgtggtc ggacggccgt
      661 acgacgtcat cctccgcgag ttcgagggtg agcggctgat cgaggccgtc acgcacgatc
      721 ccgagggcgg caccggcgac gtcaagtacc accacggctc cgagggcgtc cgccggacgg
      781 ggagcggcga ggtgcgcgtc cgcctcgcct ccaacccgag ccacctcgag gcggtcgatc
      841 ccgtcgtcgt aggcgagacc cgcgcgatgc agaccgacca cgcgagcccg gaggggagcc
      901 acgacccgac gtccgcgctc gccgtcctca tccacgggga cgccgccttc ccgggtcagg
      961 ggatcgtcgc ggagacgatg aacctccagg acctcgaggg ctactcgacc ggagggacgc
     1021 tccacctgat cgcccacaac caggtcgggt tcaccaccga tcccggcgag gggcgctcga
     1081 cgcggtactc gagcgacatc gccaagggct acgacacgcc gatcgtccac gtgaacgccg
     1141 acgaccccga ggcgtcgatc gcggcgatcc gcctcgcgat ggcgtaccgc gagcgcttcc
     1201 gccgcgacgt cgtcgtcgac ctggtcggct accggcgctt cggccacaac gagggtgacg
     1261 agcccgccta cacccaaccg ctcatgtacc gggcgatcga agaccacccg accgcgcgcg
     1321 agcagtacgc ggcctcgctc gtcgaggagg gcgtggtcac gggcgaggag gccgacgagc
     1381 tcgtccgaag cgtgcaggag cggatgcgcg aagcgcacga cgccctcaag gcctcgctcg
     1441 aggcgagcgg cgcctccgcg cgggtcgagc gaagacccgg acgcgtgggc ttcgaggagc
     1501 ccgcgaccgc cgtccccgag gagcgtctcg aagtcctgaa ccgcgagctt ctcgccgtcc
     1561 cggacgggtt cacgatccac cccaagctcg cgaagcagct cgagcgcagg ctcgagacgc
     1621 tcgggccaga gggaggcatc gactgggcgc aggccgagtc cctcgcgctc gggagcctcg
     1681 tcacggaagg cgtcccggtc cgtctgacgg gccacgacac cgagcgcgga acgttcgcgc
     1741 accgccacct cgttctccac gacgccgaga cgggcgacaa gtggacgccg atcgagcacc
     1801 tcgcggaggc gaaggcaccg ttccaggtct acaactcgcc gctctcggag gccggctgcg
     1861 tcggattcga gtacggctac gc
//
LOCUS       DASZOB010000392         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_44431, whole genome shotgun sequence.
ACCESSION   DASZOB010000392 DASZOB010000000
VERSION     DASZOB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44431"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VG709_06250"
     CDS             complement(<1..400)
                     /locus_tag="VG709_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC32713.1"
                     /translation="MRLLELSLRNYRVFERVDLELPSRVIGVFGPNGAGKSSLLESVA
                     YALYGVARTAKQDIRTHGVLTDCEVRLVFQHGARQYEVRRLIRGRNHQTDAELLAGDA
                     SVAVGVTDVDAEIRRLLRMDQQVFRSSVFAE"
     gene            complement(402..1538)
                     /locus_tag="VG709_06255"
     CDS             complement(402..1538)
                     /locus_tag="VG709_06255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HWC32714.1"
                     /translation="MARLAALGDAHLGRTMYAHVRDDHGRNVREEDFLRSFDWAIEET
                     LRLEPDGFLWLGDVFDHARPSYRTFTRVAIGLRRLADAGLRGVAISGNHDTPRLRGTG
                     SPYAALEEFFPNVTFAWRMESLTTELAGVRVHAVPQTVTVEDFKEQLDASARDAGRDA
                     TSVLLAHVALTSLPSRDWRDINELEVSEGDLDRRFDLVLLGHYHLHLKPSRRAWYAGS
                     TDAFSFADRPRGAGPKGLVLLDTDAGKVAHHANPHERPLVTFGIDSGGLGPTELIETC
                     ARQAHGTAEGAVVRVFLNAVDASAYRQLTPGDFMDAVPGALHVQVEPDYADAALSVQA
                     AVELRTLEAEWGDYVERQDLVGLDRGRVRALGARFLEDARGEAV"
BASE COUNT          230 a          601 c          598 g          220 t
ORIGIN      
        1 gctcggcgaa gaccgacgag cggaacacct gctggtccat gcgcagcagc cggcggatct
       61 cggcgtcgac gtcggtgacg ccgaccgcca cggacgcgtc cccggccagc agctcggcgt
      121 cggtctggtg gttcctgccg cggatgagac gccggacctc gtactgccgg gcgccgtgct
      181 ggaacaccag gcgcacctcg cagtcggtca ggacaccgtg cgtgcggatg tcctgcttcg
      241 cggtgcgcgc cacgccgtag agcgcgtacg ccacgctctc gagcaggctg ctcttgccgg
      301 cgccgttcgg gccgaacacc ccgatgaccc gcgaggggag ctcgaggtcg acccgctcga
      361 acacccggta gttccgcagc gacagctcga gcagccgcac ctcagaccgc ctccccgcga
      421 gcgtcctcga ggaagcgcgc gccgagcgcg cggacccggc cgcggtcgag cccgaccaga
      481 tcctgtcgct cgacgtagtc cccccactcg gcctccaggg tgcggagctc cacggcggcc
      541 tgcaccgaca gggcggcatc cgcgtagtcc ggctcgacct gcacgtgcag cgcgcccggc
      601 accgcatcca tgaagtctcc cggcgtgagc tgtcggtacg ccgacgcgtc gacggcgttc
      661 aggaagacgc ggacgacggc tccctcggcg gtcccgtgcg cctgtcgcgc gcacgtctcg
      721 atcagctccg tcggcccgag gccgccggag tcgatgccga acgtcacgag tgggcgctcg
      781 tgcgggttcg cgtggtgggc gaccttcccg gcatcggtgt cgaggaggac cagccccttc
      841 gggcccgcgc cgcgcggccg gtcggcgaag ctgaacgcgt cggtcgaacc cgcgtaccac
      901 gctcgcctcg agggcttgag atgcaggtgg tagtggccga gcagcacgag atcgaaccgg
      961 cggtcgaggt cgccctcgga gacctcgagc tcgttgatgt cgcgccagtc gcgggagggg
     1021 agcgaggtga gcgcgacgtg cgcgagcagc acgctggtcg cgtcccggcc ggcgtcgcgc
     1081 gccgacgcgt cgagctgctc cttgaagtcc tcgaccgtga ccgtttgcgg gacggcgtgc
     1141 acgcgcacac cggcgagctc cgtcgtgagc gactccatcc gccaggcgaa cgtcacgttc
     1201 gggaagaact cctcgagcgc cgcgtacggt gagccggtgc cccgcagccg cggcgtgtcg
     1261 tggttgccgc tgatcgcgac gccgcgcagc ccggcgtcgg cgaggcgccg gagcccgatc
     1321 gcgacgcggg tgaacgtgcg gtagctcggc cgcgcgtggt cgaagacgtc gcccagccag
     1381 aggaacccgt cgggctccag ccggagcgtc tcctcgatcg cccagtcgaa cgagcggagg
     1441 aagtcctcct cccgcacgtt gcgcccgtgg tcgtcgcgga cgtgcgcgta catcgtccgc
     1501 ccgaggtgcg cgtcgccgag cgcggccagc cgcgccatca gcgcacctcc gggagacagc
     1561 gtcgggcgat gcgcactact ccgcgaaccc ccgggaggcg ccctcgggcc tcggggtaag
     1621 cggagcgggg cctgggaccg acttcaggt
//
LOCUS       DASZOB010000393         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_78986, whole genome shotgun sequence.
ACCESSION   DASZOB010000393 DASZOB010000000
VERSION     DASZOB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78986"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VG709_06260"
     CDS             <1..460
                     /locus_tag="VG709_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC32715.1"
                     /translation="ALTYPFLGPGLLLLPLALVVLLLFTAAVITWTSAWNVRYRDITY
                     IVELAMLAWFWGTPIVYPTNILPDRIRDTAFFDVYLLNPLANIVLGFQRALYGGTDVP
                     RLPTGELVLPAADISWYALRLGVVGLVSLVLLYLAWRTFFRLSGDFAEEL"
     gene            464..>1630
                     /locus_tag="VG709_06265"
     CDS             464..>1630
                     /locus_tag="VG709_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32716.1"
                     /translation="MAGPAISIGRVSKRFRVFHERPSSLKERVLKFRRVRAEDFWALR
                     DVTAEVPDGRTVGLVGPNGSGKTTLLKIIGGIIRPTAGRVVTRGRIAALLELGAGFHP
                     ELTGRENVYLNASILGLSKRETDRHFDDIVAFAELEEFIDNQVKHYSSGMFVRLGFAV
                     AVHVDPDILLVDEVLAVGDEAFQRKCIARVRQFQREGRTIVFVTHAVQLIHQICDEAL
                     LLERGHLRASGDVEGVIRELRRVMLRRRTESREDLTYDMEIVGVELLDATGRRSSVLR
                     PGDGLGVQVELRANRAVPGPVVGIAINDANDTHVFGTSTQKERADVERLEAGEKVRVT
                     FQLPHPPLVEGRYHVTVRARNLDQTIWYDWHDKEYTFDVARGLDEDAAIEIPTEV"
BASE COUNT          245 a          548 c          566 g          271 t
ORIGIN      
        1 ggccctcacg tacccgttcc tcggaccggg gctgctgctg ctcccgctcg cgctggtcgt
       61 ccttttgctg ttcaccgcag ccgtcatcac gtggacgtcc gcgtggaacg tgcgataccg
      121 cgacatcacg tacatcgtcg agctcgcgat gctcgcgtgg ttctggggca cgccgatcgt
      181 gtacccgacg aacatcctcc ccgacaggat cagggacacg gcgttcttcg acgtctacct
      241 gctgaacccg ctcgcgaaca tcgtgttggg gttccagcgc gcgctttacg gagggacgga
      301 cgtcccgcgg cttccgaccg gcgagctcgt cctgcccgcg gcggacatct cgtggtacgc
      361 gctccgcctc ggcgtcgtgg ggctcgtgtc gctcgtgctc ctgtacctcg catggcggac
      421 gttcttccgc ctgtcgggag atttcgccga ggagctctga gccgtggccg ggccggcgat
      481 ctcgatcggg cgggtctcga agcgcttccg cgtcttccac gagcgtccgt cgagcctgaa
      541 ggagcgtgtc ctgaagttcc ggcgcgtgcg ggcggaggac ttctgggcgc tacgcgacgt
      601 cacggccgaa gttcccgacg gccgaacggt cgggctcgtc ggcccgaacg gctccgggaa
      661 gacgacgctg ctcaagatca tcggcgggat catcaggccg acggcgggac gcgtcgtgac
      721 gcgaggccgg atcgccgcgc tcctcgagct cggcgccggg ttccaccccg agctcaccgg
      781 gcgcgagaac gtctacctga acgcgtcgat ccttgggctc tcgaagcgag agaccgaccg
      841 ccatttcgac gacatcgtcg cgttcgccga gctcgaggag ttcatcgaca accaggtgaa
      901 gcactactcc tcggggatgt tcgtccggct cgggttcgcc gtcgctgtcc atgtcgatcc
      961 ggacatcctc ctcgtcgacg aggtgctcgc ggtcggcgac gaggcgttcc agcgcaagtg
     1021 catcgcacga gttcggcagt tccagcgcga gggacgcacg atcgtcttcg tcacgcacgc
     1081 ggtccagctc atccatcaga tctgcgacga ggcgctgctg ctcgagcgcg ggcacctccg
     1141 agcgtccggc gacgtggagg gcgtgatcag ggagctgcgg cgggtgatgc tgcgccggcg
     1201 aaccgagtcg cgcgaggatc tcacgtacga catggagatc gtcggcgtgg agctcctcga
     1261 cgcgacgggc cgccggagct cggtcctgcg tccgggcgac ggcctcggcg tgcaggtgga
     1321 gctgcgcgcg aaccgagcgg tcccgggccc ggtcgtcggg atcgccatca acgacgccaa
     1381 cgacacgcac gtgttcggga cgagcacgca gaaggagcgt gcggacgtcg aacggctcga
     1441 ggccggggag aaggtccgcg tgacgttcca gctgccgcac ccgccgctgg tcgagggccg
     1501 ctatcacgtg acggtgcgcg cgcggaacct cgaccagacc atctggtacg actggcacga
     1561 caaggagtac acgttcgacg tcgcgcgcgg cctcgacgag gacgccgcga tcgagatccc
     1621 gaccgaggtg
//
LOCUS       DASZOB010000394         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_135246, whole genome shotgun sequence.
ACCESSION   DASZOB010000394 DASZOB010000000
VERSION     DASZOB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135246"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VG709_06270"
     CDS             complement(<1..328)
                     /locus_tag="VG709_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWC32717.1"
                     /translation="MEGEGVIRFPTERVERAGSFDDFFEEEHERLFKALYFVTGNRHD
                     AEELMQEAFLKMWERWDERHRITDPTGYLFRVALNGFRMRRRRAATAIRKAVPVAERR
                     DDFLDAE"
     gene            complement(366..1559)
                     /locus_tag="VG709_06275"
     CDS             complement(366..1559)
                     /locus_tag="VG709_06275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWC32718.1"
                     /translation="MRDPYDVIVVGARCAGSPLAMLLARRGYRVLLLDRGHFPSDTVS
                     SHYVQQAATARLERWGLLDAVRASGCPPIYEMDFDIDGIRFRGSAPTDGPREAFSVRR
                     HVLDAILVEAAVEAGAELRDRFTVKDVVWEDGRVVGSRGRRSGGRDVSERARLVVGAD
                     GLRSTVAKAVSARVYGERPPLTHQYYTYWSGLPQDALGLYTAPWIGAGVAPTNDGLTI
                     VNIAWSVREFPDVRDDIEGRYLDAMRALPPLADRLDGARREERFSGMPNVPMFFREPA
                     SPGWALAGDAGYHKDPVPAQGITDAFRDADAIAETVRRDLEDGDANAWADFARRRDEV
                     ALPFYRWTRSVASLEPLAEPARRLLEAVASDRTMSDRYAGLFAETVGPDDFFEFVPPE
                     RSGIA"
     gene            complement(1559..3190)
                     /locus_tag="VG709_06280"
     CDS             complement(1559..3190)
                     /locus_tag="VG709_06280"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWC32719.1"
                     /translation="MADAADPTAPTGATPREMPTLTYAGPDMRGHVEELREKKARFRE
                     MGGRDRVERQHREGKLTVRERLDRLFDPGSFSELSLLAHHQSSSPAMQGKATPADGCI
                     TGVGLVEGRRVAVIAYDFTVMAGSIGMVGEFKATRMRELALRERIPLVWLIDSAGARI
                     QETSGSMFARTGELFREQVHMSGVVPQVAAMMGPGAAGTAYIPGLADFVPMVKGTSHM
                     ALGGPPLVKAAVGEDVTAEEMGGSQGHTKVSGVADLEVADDEECLRAVRSYLSYFPSS
                     NLERPPVVETDDPDDRRTDDLLDVVPANPRQAYDVRKVIRSIVDDGEFFAMKPEWARN
                     LVTGFARFAGRPVGIVANQPMFLGGALDVNAADKAARFVWLCDAFHVPLVFLMDCPGF
                     LVGSAVEKQGIIRHGAKMLFAVAEATVPKITVVLRKGYGAGYYVMNGRAYEPDLIVAW
                     PTAEISVMGPEGAVNIIFRRQIEAAPEEQRDAIRDQMIETVREQINAYIAAGWSFVDD
                     LIEPGETRGAICKGLALAQNKKVERPWRKHGVLPV"
BASE COUNT          504 a         1253 c         1126 g          514 t
ORIGIN      
        1 tctcggcgtc gaggaagtcg tcgcggcgct ccgcgaccgg caccgccttc cggatcgccg
       61 tcgccgcgcg gcgccgacgc atccggaacc cgttcagcgc gacgcggaac agatagccgg
      121 tggggtctgt gatgcggtgc cgctcatccc accgttccca catcttcagg aacgcttcct
      181 gcatcagctc ctccgcgtcg tgccggttgc cggtcacgaa gtacagggcc ttgaagaggc
      241 gctcgtgctc ctcctcgaag aagtcgtcga agctgccagc tcgttcgacc ctctccgtgg
      301 ggaaccgtat gaccccctct ccctccactc gcatgagcca tggatgcacg acgagccccg
      361 aacgcttacg cgatcccgga cctctcaggc ggtacgaact cgaagaagtc gtccgggccg
      421 acggtctcgg cgaagaggcc cgcgtagcgg tcggacatcg tgcggtccga cgcgacggcc
      481 tcgagcaacc gccgagccgg ctccgcgagc ggctcgagcg acgcgaccga ccgggtccag
      541 cggtaaaagg gcagcgcgac ctcatcgcgt cgccgcgcga aatccgccca cgcgttcgcg
      601 tctccgtcct cgaggtcgcg gcggacggtc tcggcgatgg cgtcggcgtc gcggaaggcg
      661 tcggtgatcc cctgggccgg aacgggatcc ttgtggtagc cggcgtcgcc ggcgagcgcc
      721 caaccggggc tggccggctc gcggaagaac atcgggacgt tcggcatccc ggagaaccgc
      781 tcctctcgcc tcgccccgtc gaggcggtcc gcgagcggag ggagcgcgcg catcgcgtcg
      841 aggtaacgac cctcgatgtc gtcgcgcacg tcggggaact ctcgcacgga ccacgcgatg
      901 ttgacgatcg tgagaccgtc gttcgtcggc gcgacgccgg caccgatcca cggcgccgta
      961 tacaggccga gcgcgtcctg cggaaggccg ctccagtacg tgtagtactg atgggtgagc
     1021 ggcggccgct caccgtacac tcgcgcgctc acggccttcg ccaccgtcga gcggaggccg
     1081 tccgcgccga cgacgagacg cgcgcgttcg ctgacgtccc gtcctccgct ccggcggccg
     1141 cggctcccga cgacacggcc gtcctcccag acgacgtcct tcacggtgaa gcggtcccgg
     1201 agctcggccc cggcctccac cgccgcttcc acgaggatcg cgtccagcac atggcgccgc
     1261 accgagaacg cctccctggg cccatcggtc ggggcgcttc cacggaaccg gatgccgtcg
     1321 atgtcgaaat ccatctcgta gatcggcgga catccagacg cccggaccgc atcgaggagg
     1381 ccccaccgct cgagacgcgc ggtcgccgcc tgctggacgt agtgactcga cacggtgtcg
     1441 ctcgggaaat gcccccggtc gaggagcagc acgcggtacc cacgccgcgc gagcagcatg
     1501 gcgagtggcg agcccgcaca ccgcgccccg accacgatca cgtcgtacgg gtcgcgcatc
     1561 acaccggaag gacgccgtgc ttgcgccagg ggcgctccac cttcttgttc tgcgccagcg
     1621 cgaggccctt gcagatcgcg ccgcgggtct cccccggctc gatgaggtcg tcgacgaacg
     1681 accatcccgc ggcgatgtac gcgttgatct gctcgcggac cgtctcgatc atctggtcac
     1741 ggatcgcgtc gcgctgctcc tccggcgccg cctcgatctg cctgcggaag atgatgttca
     1801 cggcgccctc aggacccatc acagagatct ccgcggtcgg ccacgccacg atgaggtccg
     1861 gctcgtacgc gcggccgttc ataacgtagt acccggcgcc gtagcccttg cgcagcacga
     1921 ccgtgatctt cgggaccgtc gcctccgcga ccgcgaacag catcttggcg ccgtggcgga
     1981 tgatcccctg cttctcgacg gccgagccga cgaggaaccc cgggcagtcc atcaggaaga
     2041 cgagcggcac gtggaacgcg tcgcacagcc acacgaaccg cgcggccttg tcggcggcgt
     2101 tcacgtcgag cgcgccgccg aggaacatcg gctggttcgc gacgatcccc acgggccgtc
     2161 ccgcgaaccg cgcgaacccg gtcacgaggt tccgcgccca ttcgggcttc atcgcgaaga
     2221 actcgccgtc gtcgacgatc gagcggatca ctttccgaac gtcgtacgcc tgtcgtgggt
     2281 tcgccggcac gacgtccagg aggtcgtcgg tccgccggtc gtccggatcg tcggtctcga
     2341 cgaccggcgg gcgctcgagg ttcgaggatg ggaaatacga caggtagctc cggacggcgc
     2401 gaaggcactc ctcgtcgtcg gcgacctcga ggtccgcgac gcccgagact ttcgtgtggc
     2461 cctgcgagcc ccccatttcc tccgccgtga cgtcctcgcc gaccgcggcc ttgacgagcg
     2521 gcgggccgcc gagcgccatg tgcgacgtcc ccttcaccat cggaacgaag tcggcgagcc
     2581 ccgggatgta cgcggtcccc gccgcgccgg gacccatcat ggcggcgacc tgcgggacga
     2641 cgccgctcat gtgcacctgc tcgcggaaga gttcgccggt gcgcgcgaac atcgatccgg
     2701 atgtctcctg gatccgcgcc ccggccgagt cgatcagcca gacgagcggg atgcgctctc
     2761 gcagggcgag ttcccgcatc cgtgtcgcct tgaactctcc gaccatcccg atggagccag
     2821 ccatcaccgt gaagtcgtac gcgatcaccg cgacgcggcg cccctcgacg aggcccactc
     2881 cggtgatgca gccgtcggca ggcgtcgcct tcccctgcat cgccggtgag gacgattggt
     2941 gatgcgccag cagcgacagc tcggaaaagg aaccggggtc gaagagccgg tcgaggcgct
     3001 cgcgaacggt gagcttcccc tcccggtgct ggcgctcgac gcggtcgcgg ccgcccatct
     3061 cgcggaagcg tgccttcttc tcgcggagct cctcgacgtg cccgcgcatg tccgggcccg
     3121 catacgtgag cgtcggcatc tcgcggggcg tggcgccggt cggcgccgtg gggtcggccg
     3181 cgtcggccat ggggcacctc ctcggggccc ttcgccggga gcggtcctcc cgatgcttgc
     3241 ccgggcccgc cgccgcgagt ctagcccgct aggtcgactt ccagcggggc tgacgcttct
     3301 cgaggaacgc cgagactcct tcgacgaggt cgtcctcctc gaggttctcc gccagcatct
     3361 ccttcaggtg ctggaccgcc ggcccgaacc ccaggcc
//
LOCUS       DASZOB010000395         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_159819, whole genome shotgun sequence.
ACCESSION   DASZOB010000395 DASZOB010000000
VERSION     DASZOB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159819"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VG709_06285"
     CDS             <1..376
                     /locus_tag="VG709_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWC32720.1"
                     /translation="RDEAGHPTFVDLSDAYDGPLADRAVSAAREVGIAVSRGVYAAVA
                     GPSFETRAETEVYREAGATVVGMSVVPEVAAARALGMRVLGIWCVTNRVGAGVTHEEV
                     LRVAGAAAEPLGRVLERVVAEA"
     gene            390..1643
                     /gene="ahcY"
                     /locus_tag="VG709_06290"
     CDS             390..1643
                     /gene="ahcY"
                     /locus_tag="VG709_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWC32721.1"
                     /translation="MDCDVKDLGLAEKGRERIDWAAREMKVLELIRQRFEKERPLEGI
                     RVGACLHVTSETANLLETLRAGGAEVAVCASNPLSTQDDVAAALCEQDGIAAVAIKGE
                     DDETYYRHIREVLDRRPHITMDDGADLVSTLHRERAEQLSELRGGTEETTTGVIRLRS
                     MAASGVLGYAIVAVNDAQTKHLFDNRFGTGQSTIDGILRATNLLLAGRVVVVCGYGMV
                     GRGVASRARGFGAQVVVTEVEPVPALEAAMEGYRVMPLREAARVGDLFITVTGDTSVI
                     RDEHIDVMRDGAILANSGHFDVEIDKGALDERSVSRRRIREFVDEYRLADGRRIFLLG
                     EGRLVNLAAAEGHPAAVMDMSFANQALCAEWIVKQAGLAPAVYEVPAEIDAEVARLKL
                     QGMGIEIDELTDEQREYLSSWELGT"
     gene            1660..2292
                     /locus_tag="VG709_06295"
     CDS             1660..2292
                     /locus_tag="VG709_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32722.1"
                     /translation="MTAAPAALDVVFERFPASVRGAVIVRGLDADPHQVAISEIAAVE
                     AADPSRLRTALDLEPVSVDVIPRGEVVVPFDVPFASLEPGWYRIEVEGLVDGQWRVRG
                     PVDHKTFVVPWPAEDVRRGHVTVGRSVGGAEIERVESRSDRSIVRWRAEGDADLRIVV
                     GRHRLPLIERPDPSTGERTTIAYPLLKRYTSLTLEVDRGRERSATIIELP"
     gene            2341..>2792
                     /locus_tag="VG709_06300"
     CDS             2341..>2792
                     /locus_tag="VG709_06300"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HWC32723.1"
                     /translation="MGLLDVVFPPKCAACGRPGWPLCERCADGVAVVVPPLCSRCGSP
                     TEEPLRSCVECPPREIDTVRSPFVYEGPIAGAIRGMKFSGWRALGAHLAAAMAEVAGE
                     ARADVVTWVPLSRRRRARRGFDQAEVLARGVASAVGLPARRLLTRRRNT"
BASE COUNT          375 a          940 c         1079 g          398 t
ORIGIN      
        1 gcgggatgag gccggccacc cgacgttcgt cgacctctcg gacgcgtacg atgggccgct
       61 ggcggaccgc gcggtctccg ccgcgcgcga ggtcgggatc gcggtgtcgc gcggcgtgta
      121 cgccgcggtg gcgggtccga gcttcgagac gcgcgcggag accgaggtgt accgcgaggc
      181 cggcgcgacc gtcgtcggta tgtcggtcgt cccggaggtc gcggccgcga gggcgctcgg
      241 gatgcgggtg ctcgggatct ggtgcgtgac gaaccgcgtg ggcgccgggg tgacgcacga
      301 agaggtgctg cgcgtcgccg gcgcggccgc cgagccgctc ggccgcgtgc tcgagcgtgt
      361 cgtggcggag gcctgagagg agcgagcgga tggactgcga cgtgaaggat ctggggctgg
      421 cggagaaggg gcgcgagcgc atcgactggg ccgcgcgcga gatgaaggtg ctcgagctga
      481 tccggcagcg gttcgagaag gagcgaccgc tcgaagggat ccgcgtcggg gcgtgcctcc
      541 acgtcacgtc ggagaccgcg aaccttctcg agacgctccg ggcgggaggc gccgaggtcg
      601 cggtgtgcgc atcgaacccg ctgtcgacgc aggacgacgt cgccgccgcg ctctgcgagc
      661 aggacggcat cgccgcagtc gctatcaagg gtgaggacga cgagacctac taccgccaca
      721 tccgcgaggt gctcgacagg cggccgcaca tcacgatgga cgacggcgcg gacctggtct
      781 cgacgcttca ccgtgaacgc gcggaacagc tctcggagct ccggggcggg accgaggaga
      841 ccacaacggg cgtgatccgg ctccgctcga tggcggcaag cggcgtcctc gggtacgcga
      901 tcgtcgcggt caacgacgcg cagacgaagc acctgttcga caaccgcttc gggacggggc
      961 agtcgacgat cgacgggatc ctgcgcgcga cgaacctgct gctcgccggg cgcgtcgtcg
     1021 tcgtgtgcgg atacggcatg gtcgggcgcg gcgtcgcctc acgcgcccga ggcttcggcg
     1081 cgcaggtcgt cgtgacggag gtcgagccgg tcccggcgct cgaggcggcg atggagggat
     1141 accgggtcat gccgctgcgc gaggccgctc gcgtcggaga tctgttcatc acggtcaccg
     1201 gcgacacgag cgtgatccga gacgagcaca tcgacgtcat gagggacggc gcgatcctcg
     1261 cgaactccgg gcacttcgac gtcgagatcg acaagggcgc gctcgacgag cgctcggtgt
     1321 cgcgacgacg gatccgcgag ttcgtcgacg agtaccggct cgccgacggc cgccggatct
     1381 tcctgctcgg tgaggggcgg ctcgtgaacc tcgccgcggc cgagggtcac cccgccgccg
     1441 tgatggacat gtcgttcgcg aaccaggcgc tgtgcgccga gtggatcgtg aagcaggccg
     1501 gtcttgcccc ggccgtgtac gaggtgccgg ccgagatcga cgccgaggtc gcgcggctga
     1561 agctccaggg gatgggcatc gagatcgacg agctcaccga cgagcagcgc gagtacctgt
     1621 cgtcgtggga gctggggacg tagctgcgga cgcggtccga tgaccgccgc ccccgcggcc
     1681 ctcgacgtcg tcttcgagcg ctttcccgcg tcggtgcgcg gagccgtgat cgtccgcggc
     1741 ctcgacgccg acccgcatca ggtcgcgatc agcgagatcg ccgccgtgga ggcggccgac
     1801 ccgtcgcgtc tccgaaccgc gctcgacctc gagccggtct cggtcgacgt gataccgcgc
     1861 ggcgaggtcg tcgtgccgtt cgacgtcccg ttcgcgtcgc tcgaacccgg gtggtaccgg
     1921 atcgaggtgg agggcctcgt cgacgggcag tggcgcgtgc gaggccccgt cgaccacaag
     1981 accttcgtcg tcccgtggcc ggccgaggac gtccgccgag gccacgtcac cgtcggtcgg
     2041 tccgtcggcg gcgccgagat agagcgcgtc gagtcgaggt ccgaccgctc gatcgtacgg
     2101 tggcgtgcgg agggcgatgc cgacctccgc atcgtcgtgg ggcgtcaccg gctgccgttg
     2161 atcgagcgcc cggacccgtc gacgggggag cggacgacga tcgcgtaccc gctcctcaag
     2221 cgctacacat cgctcacgct cgaggtggat cggggccgcg agcgatccgc gaccatcatc
     2281 gagcttccgt agagccgtcg cggcgcgcgg cgtgtcggac cgtcccgcta cggtgcggcc
     2341 gtggggctcc tcgacgtcgt cttccccccg aagtgcgcgg cgtgcggccg ccccggctgg
     2401 ccgctctgcg agcggtgcgc cgacggcgtt gccgtcgtcg tgccgccgct ctgctcgcgg
     2461 tgcgggagcc cgaccgagga gccactgcgt tcctgcgtcg agtgtccgcc gcgcgagatc
     2521 gacacggtgc gttcgccgtt cgtctacgag gggccgatcg ccggagcgat ccgcgggatg
     2581 aagttctccg gctggcgggc gctcggagcg catctcgcgg cggcgatggc ggaggtcgca
     2641 ggcgaggctc gcgcggacgt cgtcacgtgg gttccgctct cgcggcgccg ccgtgcgcga
     2701 cggggattcg atcaggccga ggtgctggcg cgcggcgtcg cgtccgccgt cggcctcccc
     2761 gccaggcgcc tgctcacgcg gcgccgcaac ac
//
LOCUS       DASZOB010000396         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_58925, whole genome shotgun sequence.
ACCESSION   DASZOB010000396 DASZOB010000000
VERSION     DASZOB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58925"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VG709_06305"
     CDS             complement(<1..628)
                     /locus_tag="VG709_06305"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWC32724.1"
                     /translation="MIEAKRVFDAPTPEDLRRFTEEMPNCRLTEFGNVNVQTRVTSRS
                     AGSTYVVSDDPSVSSGKTMTREEYERIARMQDEYIRGQEMVVVDGYIGNVEDFRTRAR
                     LSIERANANIAGMQQKLYYPRENGEPEVQVIYTPNLPAPGYPDDRVIAVDLEKGVTRV
                     LNSDYFGESKKGGLRMWNNIVFERGGLALHAGCKVIPVDGRARTMLIVG"
     gene            complement(723..929)
                     /locus_tag="VG709_06310"
     CDS             complement(723..929)
                     /locus_tag="VG709_06310"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HWC32725.1"
                     /translation="MPTINTLELEGISESSWGDAAREALREAAKTIRHINRLEVLGTG
                     AEVEGDQIVRYRTQVRLYFQVESR"
     gene            972..1973
                     /gene="thiL"
                     /locus_tag="VG709_06315"
     CDS             972..1973
                     /gene="thiL"
                     /locus_tag="VG709_06315"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWC32726.1"
                     /translation="MDLTEDELVRAIRKVLSGDAPGVVVAVGDDAAVVEPGRHDGVLT
                     ADMLIENVHFRRGRGSPTDLGYKAVVVNVSDVAAMGGSPRYAVTCVALPPSVEAPWVV
                     ELYGGMRQAAEDHGMSVIGGDTNRAGGVVIAVTVYGEVARGSAVTRAGARVGDRVVVT
                     GRLGAAAGGLRLLEADPQDVKDALGTPWERDLAEAHQRPTARVGEGQMLAQAGATAMI
                     DISDGLAIDLSRLCAESGVGARIRVDDVPVAAALGELAAVTGDDPLQMALYGGEDYEL
                     LATLAPEAVAALGERMPLQYGTSLTDIGEVTEEGLFAVDADGRESALEPRGWDHFGG"
     gene            1966..2778
                     /gene="thiD"
                     /locus_tag="VG709_06320"
     CDS             1966..2778
                     /gene="thiD"
                     /locus_tag="VG709_06320"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWC32727.1"
                     /translation="MAELPRALTVAGSDSGGGAGIQADLKTFQALGVWGMSAITAVTV
                     QNTKGVSGYEELSPDTVADQIRAVVTDIGVDAAKTGMLASAAIIEAAADALAETRVPN
                     LVVDPVFLSKHGHPLLREDAVEALRTRILPLATLVTPNLPEAGGLAGFPVATEQEMER
                     AGRAILQLGPRAVLVKGGHLDGRDATDLLVTEEGIQPFSSERVDTPHTHGTGCVLSSA
                     IAAYLARGNALADAVAHGKRFVTEAIRAALEIGEGIGPVNPAWPLRLDDQPA"
     gene            complement(2766..2915)
                     /locus_tag="VG709_06325"
     CDS             complement(2766..2915)
                     /locus_tag="VG709_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32728.1"
                     /translation="METAAWAAIGVVAAISIGTLFYVGTIDAVAARMDARFDAVDGRL
                     DRHAG"
     gene            complement(2968..3159)
                     /gene="rpmB"
                     /locus_tag="VG709_06330"
     CDS             complement(2968..3159)
                     /gene="rpmB"
                     /locus_tag="VG709_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWC32729.1"
                     /translation="MAAVCEICGRQPGFGKQVSHSHRRSNRRWVPNVQKIRAVVNGTP
                     RRVHVCAKCLKAGKVVRAV"
     gene            complement(3200..>3490)
                     /locus_tag="VG709_06335"
     CDS             complement(3200..>3490)
                     /locus_tag="VG709_06335"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWC32730.1"
                     /translation="WAREIVDVSIAYSEEVARARRVLDELFGELRIDDGLRDALIDGP
                     KVLGIEQVGEKDVVLRVVAETRPSRRPDLERELRARIKERLDDRGIKVPATS"
BASE COUNT          494 a         1226 c         1249 g          521 t
ORIGIN      
        1 ggcccacgat cagcatggtc cgcgcccggc cgtcgaccgg gatcaccttg cacccggcgt
       61 gcagcgcgag gccgccgcgc tcgaagacga tgttgttcca catccgcagg ccgcccttct
      121 tcgactcgcc gaagtagtcg gagttcagca cccgtgtgac ccccttctcg agatccaccg
      181 cgatgacgcg gtcgtcgggg taccccggtg cggggagatt cggtgtgtag atgacctgga
      241 cctcgggctc gccgttctcg cgcgggtagt agagcttctg ctgcatgccc gcgatgttcg
      301 cgttcgcccg ctcgatcgag agccgtgccc gggtgcggaa gtcctcgacg ttgccgatgt
      361 agccgtcgac gacgaccatc tcctgcccac ggatgtactc gtcctgcatg cgcgcgatgc
      421 gctcgtactc ctcgcgggtc atggtcttgc cggaggagac gctcgggtcg tcgctcacga
      481 cgtaggtgct gcccgcgctc cgggaggtga cccgcgtctg cacgttcacg ttgccgaact
      541 cggtgaggcg gcagttcggc atctcctcgg tgaagcggcg caggtcctcc ggcgtcggag
      601 cgtcgaacac acgcttcgct tcgatcacgg atcgatcctt tcgagggaag gggaaacgga
      661 ttgtaccgga ccgaagcggc ccagccgcgt agcggtgtcc gttccgtccg ctgcgcgggg
      721 agctagcgcg actcgacctg gaagtacagg cgcacctgcg tgcggtagcg gacgatctgg
      781 tcgccctcga cctcggcgcc ggtccctagg acctcgaggc ggttgatgtg gcggatggtc
      841 ttcgccgcct cacgcagcgc ctcgcgggcg gcgtcacccc acgacgactc cgagatccct
      901 tcgagctcga gcgtgttgat ggtcggcacg acccctgcat tatcgtgaac gtccgacccg
      961 aaaggaaagc cgtggatctg accgaagacg agctcgttcg cgcgatcagg aaggtgctct
     1021 ccggcgacgc gcccggcgtg gtcgtggcgg tcggcgacga cgccgcggtc gtggagcccg
     1081 ggcgtcacga cggcgtcctg accgccgaca tgctgatcga gaacgtccac ttccgccgcg
     1141 gccgcggctc gccgacggac ctcggttaca aggccgtcgt cgtcaacgtg tccgacgtcg
     1201 ccgcgatggg cgggagcccg cgctacgcgg tcacgtgcgt cgcgctgccg ccgtcggtcg
     1261 aggcgccgtg ggtggtcgag ctgtacggcg ggatgcgcca ggccgccgag gaccacggca
     1321 tgtcggtgat cggcggcgac acgaatcgtg cgggcggcgt cgtcatcgcc gtgacggtgt
     1381 acggggaggt cgcccgcggc agtgccgtca cgcgcgccgg cgcgcgggtc ggcgaccgcg
     1441 tcgtcgtgac cgggcggctt ggagccgcgg cgggtgggct ccgtctgctc gaggcggatc
     1501 cgcaggacgt caaggacgcg ctcggcacgc cgtgggagcg cgacctcgcc gaggcgcacc
     1561 agcggccgac ggcacgggtc ggtgagggac agatgctcgc gcaggccggc gcgaccgcga
     1621 tgatcgacat ctcggatggg ctcgcgatcg atctctcgcg cctctgcgcg gagagcggcg
     1681 tcggcgcccg catccgggtg gacgatgtgc cggtcgccgc ggccctcggc gagctcgcgg
     1741 ccgtgaccgg ggacgacccg ctccagatgg cgctgtacgg cggcgaggac tacgagctgc
     1801 tcgcgacgct cgcaccggag gccgtggcgg ccctcggcga gcggatgccg ctccagtacg
     1861 gcacctcgct gaccgacatc ggggaggtca ccgaggaggg gctcttcgcg gtcgacgccg
     1921 acggacggga gtccgcgctc gagccgagag ggtgggacca cttcggtggc tgagctgccg
     1981 cgggcgctca cggtcgccgg ctccgactcg ggcggcggcg ccgggatcca agccgacctc
     2041 aagaccttcc aggcgctcgg cgtctggggc atgtcggcga tcacggccgt gaccgtgcag
     2101 aacacgaagg gcgtcagcgg gtacgaggag ctctcgcccg acacggtcgc cgaccagatc
     2161 cgcgccgtcg tcaccgatat cggcgtcgac gcggcgaaga ccgggatgct cgcgagcgcg
     2221 gccatcatcg aggccgccgc cgacgcgctc gccgagacgc gcgtaccgaa cctcgtcgtc
     2281 gaccccgtct tcctctcgaa gcacggccat ccgctgctgc gcgaggacgc cgtggaggcg
     2341 ctccgcacgc gcatcctccc gctcgcgacg ctcgtgacgc cgaacctccc ggaggccggc
     2401 gggctcgccg ggttccccgt agcgaccgag caggagatgg agcgggccgg ccgcgcgatc
     2461 ctccagctcg ggccgcgtgc ggtgctcgtg aagggcggtc acctcgacgg ccgcgacgcg
     2521 acggacctcc tcgtgaccga ggaggggatc cagccgttct cttcggagcg ggtcgacacg
     2581 ccgcacacgc acgggaccgg atgcgtgctc tcgtcggcga tcgccgcata cctcgcgcgg
     2641 ggcaacgcgc tcgccgatgc ggtggctcac gggaagcggt tcgtcaccga ggcgatccgt
     2701 gcggcgctcg agatcggcga ggggatcggg ccggtcaacc ccgcgtggcc gctacggctc
     2761 gacgatcagc cggcgtgacg gtcgaggcgc ccgtctacgg cgtcgaaacg agcgtccata
     2821 cgggcggcaa cggcgtcgat cgtaccgacg tagaacagcg tcccgatcga gatcgcggct
     2881 accacaccga tcgccgccca ggccgcggtt tccatctcac gggagcgaag caccggactg
     2941 ccacacgaga gccggcggag cggagggtta gaccgcgcgg acgaccttgc cggccttgag
     3001 gcacttggcg cagacgtgca cgcggcgggg tgtcccgttc acgacggcac ggatcttctg
     3061 cacgttgggc acccaacgcc ggttcgagcg ccggtgcgag tggctcacct gcttgccgaa
     3121 tcccggctgg cgcccacaga tctcacagac cgctgccatg accggcgaat cgtagcagac
     3181 gccccggcgt cctccctggt caggacgttg ccggcacctt gatcccccgg tcgtcgagtc
     3241 gctccttgat gcgcgcgcgg agctcgcgct cgaggtccgg ccgcctcgac gggcgcgtct
     3301 cggcgacgac gcggaggacg acgtccttct cgccgacctg ttcgatgccc agcaccttcg
     3361 gtccgtcgat cagcgcgtcg cgcagcccgt cgtcgatccg cagctcgccg aacagctcgt
     3421 cgagcacgcg tcgcgcccgc gccacctcct cgctgtacgc gatcgagacg tcgacgatct
     3481 cgcgcgccca
//
LOCUS       DASZOB010000397         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_129299, whole genome shotgun sequence.
ACCESSION   DASZOB010000397 DASZOB010000000
VERSION     DASZOB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129299"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..572
                     /locus_tag="VG709_06340"
                     /pseudo
     CDS             162..572
                     /locus_tag="VG709_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(738..>1804)
                     /locus_tag="VG709_06345"
     CDS             complement(738..>1804)
                     /locus_tag="VG709_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC32731.1"
                     /translation="SERSDERLDPEHAREVLDADHAGLEDVKERIVEYIAVRKMRKER
                     GIAEDKRSGAILTLIGPPGTGKTSIGESIARATGREFVRMSLGGVRDEAEIRGHRRTY
                     IGALPGRLVRALRDAGTMNPVVMLDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHY
                     LDVELDLSQVVFIATANVAETIPGPLLDRLEVVRFDGYTVDEKTAIARGYLWPRQRER
                     NGLRDDEVIVADDVLRLVIAEYTREAGVRQLERELGTLLRKTATRIASGAVETPVEVT
                     VGVVRDALGRQKFWQESAERTAVPGVANGRLVDRDRSAGMTSGDPRGGLAQHLAELAL
                     ELAHAGLARVLGYDHPEQVV"
BASE COUNT          255 a          663 c          628 g          258 t
ORIGIN      
        1 aggtagcccc ggacgtcgtc cgcgtccacc gccaccggtg gagtcacctc accggcggcg
       61 atcttcgtcg cgaccttgcg gaggatcttg cccagctccc gctcgaggtt ccggacgccg
      121 gcctcgagga cgtcaaggac cgcatcaccg agtacatcgc cgtgcgcaag ctgcggcgcg
      181 agcgcgagat cgccgaggac aggcgctccg gggcgatcct gaccctcatc gggccgcccg
      241 gcacggggaa gacctcgatc ggcgagtcga tcgcccgtgc cacggggcgt gagttcgtgc
      301 gcatgtcgct cggcggcgtg cgcgacgagg ccgagatccg tggccatcgc cgcacctaca
      361 tcggcgcgct gccgggacgg ctcgtccgcg ccctccgcga cgccgggacg atgaacccgg
      421 tgatcatgct ggacgaggtc gacaaggtcg gcgccgactg gcgcggcgac ccctcggcgg
      481 cgctgctcga ggtgctcgac cccgcgcaga accactcctt ccgcgaccac tacctcgacg
      541 tcgagctcga cctctcgcag gtgctgttca tcgccaccgc gaacgtggcc gacacgatcc
      601 ccgggccgct gctcgatcgc atggagacga tcagcttcga cggctatacg ccggacgaga
      661 aggtggcgat cgccagcggg tacctgctgc cgcgacagct cgagcgaaac ggcctccgcg
      721 atggcgaggt cgagatctca gacgacctgc tccggatggt cgtaaccgag tacacgcgcg
      781 aggccggcgt gcgccagctc gagcgcgagc tcggcaagat gctgcgcaag accgccacgc
      841 ggatcgccgc tggtcatgcc agcggaccgg tcacgatcga cgaggcggcc gttcgcgacg
      901 cccgggaccg cggtccgctc ggcgctctcc tgccagaact tctggcgccc gagcgcgtcg
      961 cgcacgacgc cgacggtgac ctcgaccggc gtctcgacgg cgccgctcgc gatccgggtc
     1021 gcggtcttgc gcagcagcgt gcccagctcg cgctcgagct ggcgcacccc ggcctcgcgg
     1081 gtgtactcgg cgatgacgag ccgcaggacg tcgtcggcca cgatgacctc gtcgtcgcgc
     1141 aggccgttgc gctcgcgctg gcgcggccac aggtagccgc gggcgatcgc cgtcttctcg
     1201 tcgacggtgt agccgtcgaa gcgcacgacc tccaggcggt cgagcagagg gccggggatg
     1261 gtctcggcca cgttcgcggt ggcgatgaac acgacctgcg acaggtcgag ctccacgtcc
     1321 aggtagtggt cgcggaagga gtggttctgc gccgggtcga gcacctcgag cagcgcggcc
     1381 gagggatcgc cgcgccagtc ggcgccgacc ttgtcgacct cgtcgagcat gaccacgggg
     1441 ttcatcgtcc cggcgtcgcg cagcgcccgc acgagccggc ccggcagcgc gccgatgtag
     1501 gtccgccggt gaccgcggat ctcggcctcg tcgcgcaccc cgccgagcga catgcgcacg
     1561 aactcgcgtc ccgtggcgcg ggcgatcgac tcgccgatcg acgtcttgcc ggtgccgggc
     1621 gggccgatca gggtgaggat cgcgccggag cgcttgtcct ccgcgatgcc gcgctccttc
     1681 cgcatcttgc gcaccgcgat gtactccacg atccgctcct tcacgtcctc gagcccggcg
     1741 tggtcagcgt ccagcacctc gcgcgcatgc tccgggtcga gccgctcgtc ggagcgctcc
     1801 gacc
//
LOCUS       DASZOB010000398         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_159872, whole genome shotgun sequence.
ACCESSION   DASZOB010000398 DASZOB010000000
VERSION     DASZOB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159872"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VG709_06350"
     CDS             <1..1281
                     /locus_tag="VG709_06350"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWC32732.1"
                     /translation="HITDEIKSRIRGIGHETDADVVIVEVGGTVGDIESLPFLEAIRQ
                     LRNEVGRDRCAFVHLSLLPRIAPTGELKTKPTQHSVKELRGLGISPDAIVCRADEQIS
                     PALKQKISLLCDVDLDAVISEVDAERTIYDVPTMLHEEGLDDYIARILQLDHGFDFGE
                     WQALLDRIDAATEPVTIAVVGKYVDLPDAYLSVIEALNHGGYHHGAKIDVRWVASDQL
                     RDGDVDALLGDVDGILVPGGFGVRGVEGKVDAIRYARERRVPFLGICLGLQCAVIEFA
                     RNGVGLRDANSSEFEPTCEHPVIDLLPEQKDVTDLGGSMRLGAQPCHLVAGTQSLEAY
                     GERIVFERHRHRYEVNPAYRLSLEEHGLIVAGTSPDGRLVEVIELADHPFFVATQFHP
                     ELKSRPTRPHPLYRDFVGAAFARRRAPEAAAAFR"
     gene            1377..1790
                     /locus_tag="VG709_06355"
     CDS             1377..1790
                     /locus_tag="VG709_06355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWC32733.1"
                     /translation="MVRHPGAVAVVALTRDGAVVLVRQRREAVRETLLEVPAGILDVA
                     GEAPQDAARRELREETGYEADVWEPLGAILSSPGFSDERVELFAARGAERVTEPEEGI
                     EVVVMAHGEALRAVRDGTIADAKSAGALLLSAASA"
BASE COUNT          275 a          638 c          653 g          277 t
ORIGIN      
        1 cacatcaccg acgagatcaa gtcccggatc cgcggcatcg ggcacgagac cgatgccgac
       61 gtcgtcatcg tcgaggtcgg cggcaccgtc ggcgacatcg agagcctccc gttcctagag
      121 gcgatccgcc agctccggaa cgaggtcggc cgcgaccgtt gcgcgttcgt gcacctgtcg
      181 ctgctgccgc ggatcgcccc gaccggcgag ctcaagacca agccgacgca gcactcggtc
      241 aaggagctcc gcgggctcgg gatcagcccc gacgcgatcg tgtgccgcgc cgacgagcag
      301 atctctccgg cgctcaagca gaagatctcg ctgctgtgcg acgtcgatct cgacgccgtg
      361 atctctgagg tggacgccga gcgcacgatc tacgacgtgc ccacgatgct ccacgaagag
      421 gggctcgatg actacatcgc gcgcatcctc cagctcgacc acggcttcga cttcggagag
      481 tggcaggcgc tcctcgacag gatcgacgcg gcgaccgagc cggtgacgat cgcggtcgtc
      541 gggaagtacg tcgacctgcc cgacgcctac ctctccgtga tagaggcgct caaccacggc
      601 ggctaccacc acggcgcgaa gatcgacgtc cgctgggtgg cgtcggacca gctgcgcgac
      661 ggcgacgtcg acgcccttct cggggacgtg gacggaatcc tcgtccccgg cgggttcggt
      721 gtgcgtggcg tcgaggggaa ggtcgacgcg atccgctacg cgcgcgagcg tcgggtcccg
      781 ttcctcggca tctgcctcgg cctgcaatgc gcggtgatcg agttcgctcg gaacggcgtc
      841 ggtcttcggg atgcgaattc gagcgagttc gaaccgacgt gcgagcaccc cgtgatcgac
      901 ctcctgccgg agcagaagga cgtcaccgac ctcggtggct cgatgcgcct cggtgcccag
      961 ccgtgccacc tggttgccgg aacgcagtcc ctggaggcgt acggggagcg catcgtcttc
     1021 gagcggcacc gccaccgcta cgaggtcaac cccgcgtatc gcctctcgct cgaggagcac
     1081 gggctcatcg tcgcgggaac gtccccggac gggaggctgg tagaggtgat cgagctggcc
     1141 gaccacccgt tcttcgtcgc gacgcagttc caccccgagc tgaagtcgcg cccgacacgg
     1201 ccgcacccgt tgtaccgcga cttcgtcgga gccgcgttcg cgcgccggcg agctcccgag
     1261 gcggcggccg ccttccgctg acacctccca cgccggcccc cgacgagtcc agcgaggtct
     1321 tccgtgggaa gaggctcggc gtcgtcgtag agcgctgggg cgatcgcgag cgcgagatcg
     1381 tccgccatcc cggcgccgtc gccgtcgtgg ccctgacgcg ggatggtgcc gtcgtgctgg
     1441 tccgtcagcg gcgcgaggcg gtgcgcgaga cacttctcga ggtccccgcc gggatcctcg
     1501 acgtcgccgg ggaagcgccg caggacgcgg cccgacgcga gctccgggag gagaccggat
     1561 acgaggccga cgtatgggag ccgctcgggg cgatcttgtc gtcgccgggc ttctcggacg
     1621 agcgagttga gctcttcgcg gctcgtggcg ccgagcgggt cacggagccg gaggagggga
     1681 tcgaggtcgt cgtgatggcg cacggcgagg cgctccgcgc ggttcgcgat ggaacgatcg
     1741 ccgacgcgaa gagcgccggt gcgctcctgc tctctgcggc ctccgcctag cggcgcttcg
     1801 gccggaagct cgcctggagg tcctgctcgg cgaaggcgcc gga
//
LOCUS       DASZOB010000399         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_129325, whole genome shotgun sequence.
ACCESSION   DASZOB010000399 DASZOB010000000
VERSION     DASZOB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129325"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VG709_06360"
     CDS             <1..848
                     /locus_tag="VG709_06360"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWC32734.1"
                     /translation="MGAVASKAHVPSWHERDPWVPASYSRETLREALLDAGIAGVNSH
                     DRPNVLWKIKRLAEDDPTARFGLSRVDEPSPEEVLRLVAEASGISPDPNERHGLVIVD
                     PDKVIDAATVAGDRLASAARAGVRILIATGHPSGLLQLYQEIGRLLEGGGAKLLRPLE
                     GFTWEDDDGGRRRRRQIRYFRGVAVLTDRASSLHTHSPDPMERMLEEADPDLVLADHG
                     FAGAAIEAGIDTVSVADVNDPALVVARAQGRAGPVIVMDDNVQPETYWPVLQVIVSRF
                     DANAG"
     gene            complement(845..1558)
                     /locus_tag="VG709_06365"
     CDS             complement(845..1558)
                     /locus_tag="VG709_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWC32735.1"
                     /translation="MGGSLYLGTSGFAYKEWKGIFYPDELKDGEMLPYYASRFASVEI
                     NYTFRRSPAETTVRTWSERTPDGFRFALKAHQRITHTRRLDTGDEGVAWFLDRVRPLG
                     QRLGPILFQCPPTLRFDAAVLDAFLSALPEGRRYAMEFRHDSWAQARETLRERGVAWC
                     VAESDESPGAPAWLAEQPFGYLRLRKETYADADLRVWADRISEMLRSGRDVYCYLKHE
                     EEGTGPRYAERLGELLDAV"
     gene            1625..1822
                     /locus_tag="VG709_06370"
     CDS             1625..1822
                     /locus_tag="VG709_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWC32736.1"
                     /translation="MAQKSFSEARFVTVSEVARLMRVSKMTVYRLIKQGDLPAVRGGR
                     GYRMREEDVHRYLDERYTEAG"
BASE COUNT          298 a          666 c          653 g          271 t
ORIGIN      
        1 cgatgggtgc ggtggcaagc aaagcacacg tgccttcgtg gcacgaaagg gacccctggg
       61 ttcccgcctc gtattcccga gagacgctcc gcgaggcgct gctcgacgcc gggatcgccg
      121 gcgtcaactc ccacgaccga ccgaacgtcc tttggaagat caagcggctg gctgaggacg
      181 acccgaccgc gcggttcggg ctgagccgcg tcgacgagcc ctctcctgag gaggtgctgc
      241 ggctcgtcgc cgaggcgagc ggcatctcgc ccgacccgaa cgagcggcac ggtctcgtga
      301 tcgtcgaccc ggacaaggtg atcgacgccg cgaccgtcgc cggcgaccgt ctcgcgagcg
      361 ccgcgcgcgc gggcgtccgc atcctgatcg cgaccggcca cccctccggg ctgctccagc
      421 tctaccagga gatcggaaga ctcctcgagg gcggcggcgc gaagctcctg cgcccgttgg
      481 aggggttcac atgggaggac gacgacggcg gtcgccggcg ccgccggcag atccgctact
      541 tccgcggtgt cgcggtcctc acggaccgcg cgtcgagcct ccacacgcac agccccgatc
      601 cgatggagcg catgctcgag gaggccgacc ccgatctcgt cctggccgat cacgggttcg
      661 ccggagccgc gatcgaggcc ggcatcgaca ccgtctcggt cgccgacgtg aacgatcccg
      721 cgctcgtcgt ggcgagggcg cagggccgtg ccggaccggt gatcgtcatg gacgacaacg
      781 tccagcccga gacgtactgg ccggtcttgc aggtgatcgt gtcgcggttc gacgcgaacg
      841 cgggctaaac ggcgtccagc agctccccga ggcgctccgc atagcgcggc ccggtgccct
      901 cctcttcgtg cttcaggtag cagtagacgt cgcgccccga ccggagcatc tcgctaatcc
      961 ggtcggccca gacgcgcagg tcggcgtccg cgtaggtctc tttgcgcagt cgtagatagc
     1021 cgaacggctg ctcggcaagc caagcgggcg cgccgggcga ctcgtcggac tccgcgacgc
     1081 accacgcgac gccgcgctcg cgcagcgtct cacgcgcttg tgcccacgag tcgtgccgga
     1141 actccatcgc gtatcggcgc ccctcgggca gcgccgacag gaacgcgtcg agcaccgcgg
     1201 catcgaaccg cagtgtcggg gggcactgga acaggatcgg cccgaggcgc tgtcccagtg
     1261 gccgtacgcg gtcgaggaac cacgcgacgc cctcatcccc ggtatccagt cgccgcgtat
     1321 gcgtgatccg ctggtgcgcc ttgagcgcga accggaatcc gtcgggcgtc cgctcgctcc
     1381 acgtgcggac cgtcgtctcg gccggcgacc ggcggaacgt gtagttgatc tcgacagagg
     1441 cgaagcgcga cgcgtagtag gggagcatct cgccgtcttt cagctcgtcg ggatagaaga
     1501 tgcccttcca ttccttgtac gcgaagcccg acgtaccgag atagagcgat ccgcccacgt
     1561 ccgcgatcct cgcacgccgc gcccgtggcc ccggaaatgc ccggtcggct agaatccgcg
     1621 cagcgtggcc cagaaatcgt tcagtgaagc tcgtttcgtg accgtgtcgg aggtcgcgcg
     1681 gctgatgcgc gtctccaaga tgaccgtcta ccgcctgatc aaacaggggg acctgccggc
     1741 cgtcaggggg ggccgggggt accgcatgcg ggaagaggac gtccaccgct acctggacga
     1801 gcggtacaca gaagccggat agcggaccgg cgcacggccg ggcgcacccg cggccgcgga
     1861 gccgagcttc tccggacggg cggggccc
//
LOCUS       DASZOB010000400         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_59040, whole genome shotgun sequence.
ACCESSION   DASZOB010000400 DASZOB010000000
VERSION     DASZOB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59040"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VG709_06375"
     CDS             complement(<1..966)
                     /locus_tag="VG709_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWC32737.1"
                     /translation="MATLTPVSGRGGLDDHGINPRGRIFWNPSTPTLYTHALAVGNAR
                     LAEGGPLVVDTGRHTGRSPDDRFIVREPESEDRIGWGAANRPIDEERFETLREKVVAH
                     LEQGDVYVVDAFAGADPAHRLGVRVVTDSAWHALFAKTLFIEPGDDELEEHRADALVL
                     HAPAVTADPAENGTRSETFVVLHPTRGEVVIGGTEYAGEIKKSIFTLMNDRLPLEGVL
                     PMHCSANVGDDDRVAIFFGLSGTGKTTLSADPERHLIGDDEHGWGDEGIFNIEGGCYA
                     KVIRLSKEAEPEIYRATHAFGTVLENVITDECGCLDLDDDSKTENT"
     gene            complement(1264..>1525)
                     /locus_tag="VG709_06380"
     CDS             complement(1264..>1525)
                     /locus_tag="VG709_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32738.1"
                     /translation="AHELALYEAAGLRSFRVLRGPAEWRRGEAAPDARADVLLSHGFE
                     VSGDPRVFYGRGGSFELDVPARKVSVPRKRGDSVDRHERVLP"
BASE COUNT          225 a          535 c          535 g          230 t
ORIGIN      
        1 cgtgttctcc gtcttcgagt cgtcgtcgag atcgaggcac ccgcactcgt cggtgatcac
       61 gttttcgagg accgtcccga acgcgtgcgt cgcgcggtag atctcaggct ccgcctcctt
      121 ggagaggcgt atgacctttg cgtagcagcc cccttcgatg ttgaagatcc cctcgtcgcc
      181 ccagccgtgc tcgtcgtcgc cgatcaggtg gcgctctggg tcggccgaga gcgtcgtctt
      241 tccggtgccc gagaggccga agaagatcgc gacgcggtcg tcgtcgccga cgttcgccga
      301 gcagtgcatg ggcaggactc cttcgagcgg gagccggtcg ttcatcagcg tgaagatcga
      361 cttcttgatc tcgcctgcgt actccgtgcc gccgatgacg acctcgcccc gcgtcgggtg
      421 gagcaccacg aaggtctcgc tccgcgtgcc gttctcggcc ggatcggccg tgacggcggg
      481 cgcatgcagg acgagcgcgt cggcccggtg ctcctcgagc tcgtcgtctc ccggctcgat
      541 gaagagcgtc ttcgcgaaga gcgcatgcca cgcggagtcg gtcacgaccc ggacgccgag
      601 ccggtgggcg gggtccgcgc cggcaaacgc gtcgaccacg tagacgtcgc cctgctcgag
      661 atgcgcgacg accttctcgc gaagcgtctc gaacctctcc tcgtcgatcg gccggttggc
      721 ggcgccccaa ccgatccggt cctccgactc cggctcgcgg acgatgaacc ggtcgtcggg
      781 cgagcgaccc gtgtggcgac cggtgtcgac gacgagcggc ccgccttcgg cgagacgggc
      841 gttcccgacg gcgagcgcgt gcgtgtacag cgtcggagtc gacgggttcc agaagatgcg
      901 cccgcgcggg ttgatcccgt ggtcgtcgag gccgccgcgg ccgctaacgg gcgtcaaggt
      961 cgccatacag gcgccctcct acccggtcgg cgcaaagcga caaactgccg cctatcgtat
     1021 gccgccggga gccggccggc cggcgtcagt cgagcgctgc gagcaaggcg acgagcgcga
     1081 ggaagcacga gcccgtgccg aacgagacgc tccagagcgc gactccgacc caatcgcttc
     1141 gaagcagggg cgctgacgtg agcccggcgg cccgtggatc ccgtgcgatc gccacgtgcg
     1201 aaggatcggt cgcggggcgg acgaaggcac cccccattcg gggagttggg cacgccggct
     1261 cgctcagggc aggactcgct cgtggcggtc cacggagtcg ccgcgctttc ggggtaccga
     1321 gaccttacga gccggaacgt cgagctcgaa cgaccctccg cgcccgtaga agacgcgcgg
     1381 atcgccgctg acctcgaacc cgtgcgacag gaggacgtcc gcccgcgcgt ccggcgccgc
     1441 ctcgccgcga cgccattcgg ccggcccgcg gaggaccctg aaggagcgca gcccggcggc
     1501 ctcgtacaac gcgagctcgt gggcg
//
LOCUS       DASZOB010000401         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_159950, whole genome shotgun sequence.
ACCESSION   DASZOB010000401 DASZOB010000000
VERSION     DASZOB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159950"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..572)
                     /locus_tag="VG709_06385"
     CDS             complement(39..572)
                     /locus_tag="VG709_06385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32739.1"
                     /translation="MVDVRTAHTADVDEPTLAAARRLLDDVFEGEMSDEDWEHALGGV
                     HALVWEGPDLVGHASVVQRRLLHRDRALRTGYVEGVGVRADRRRRGHGGAMMEAIERV
                     IRGAYELGALGATEDAVQFYAGRGWKRWRGSTSTLTPNGIVRNEDDDGCVYVLPVAAP
                     LDLDGELVCDWRDGDVW"
     gene            complement(679..>1753)
                     /locus_tag="VG709_06390"
     CDS             complement(679..>1753)
                     /locus_tag="VG709_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWC32740.1"
                     /translation="LYVTEALDRVDAEEAGPGELIAVAGMPQVTIGETLADPDDPRPL
                     PVIGVDEPSLSMTIGINTSPLSGRDGDRLTASMIRARLEQELVGNVSLRVLPTERPDA
                     WDVQGRGELQLAVLVELMRREGFELTVGKPQVVTREIDREVHEPVERLAINVPEEYVG
                     VITQLLALRKGRLEHMVNHGTGWVRMEYLAPARGLIGFRTEFLTETRGTGILHHVFDG
                     YEPWHGGLRTRPTGSLVADRRGFATSFALLNLQERGQLFVAPGIEVYEGMIVGVNARA
                     EDLDVNATKEKKLTNMRSSTAEELVRLIPPRPLSLEQALELIRDDECVEVTPSHVRLR
                     KVELSAQKRQTASSRKARGLARV"
BASE COUNT          243 a          656 c          593 g          261 t
ORIGIN      
        1 ctcctcgacc gatcgaggcg taccgacgcc gcacggccct accagacatc gccgtcgcgc
       61 cagtcgcaga cgagttcccc gtcgaggtcg agcggcgcgg cgaccggtag cacgtagacg
      121 cagccgtcgt cgtcctcgtt ccgcacgatg ccgttgggcg tgagcgtcga cgtcgagccc
      181 ctccagcgct tccacccgcg ccccgcgtag aactgcaccg cgtcctcggt ggcgccgagc
      241 gcaccaagct cgtaggcgcc gcgaatcacg cgttcgatcg cttccatcat cgcgccgccg
      301 tgcccgcgtc ggcgccggtc cgctcgcacc ccgactccct ccacgtagcc ggtgcggagg
      361 gcgcggtcac ggtggagcag ccgcctctgg acgaccgagg catgtcctac caggtcaggg
      421 ccctcccaga cgagggcgtg cactccgccc agggcatgct cccaatcctc gtcggacatc
      481 tctccctcga acacgtcgtc gaggagcctt cgcgccgccg cgagcgtcgg ctcgtcgacg
      541 tcggccgtat gggcggtacg aacgtcgacc acaggggatc ctctcgcgcc gctacgccgg
      601 aggcgaggcg agatccgcag acgaagtccg cggcggtcga gagtccgcct tcctcggcgt
      661 catcggaagc ggcgctactc agacgcgcgc caggccgcgc gccttgcgcg acgacgccgt
      721 ctggcgcttc tgcgcggaga gctcgacctt gcgcaggcgt acgtggctcg gcgtcacctc
      781 gacgcactcg tcgtcgcgga tcagctcgag cgcctgctcc agcgacagcg gtcgcggcgg
      841 gatcaagcgg acgagctcct cggcggtcga cgagcgcatg ttcgtcagct tcttctcctt
      901 cgtcgcgttg acgtccagat cctcggcgcg ggcgttcacg ccgacgatca tcccctcgta
      961 gacctcgatg ccgggggcga cgaacagctg gccgcgctcc tgcaggttga gcagcgcgaa
     1021 cgacgtcgcg aagccgcgtc ggtccgcgac cagcgagccg gtcgggcgcg tacggaggcc
     1081 gccgtgccag ggctcgtagc cgtcgaagac gtggtggagg atcccggtgc cgcgggtctc
     1141 cgtcaggaac tccgtgcgga agccgatcag cccccgcgcg ggcgccagat actccatccg
     1201 aacccagccg gtgccgtggt tgaccatgtg ctcgaggcgg cccttgcgca gcgcgagaag
     1261 ctgcgtgatc acgccgacgt actcctcggg cacgttgatc gcgaggcgct cgaccggctc
     1321 gtgcacctcc cggtcgatct cgcgcgtgac gacctgcggc ttgccgacgg tgagctcgaa
     1381 gccctcgcgg cgcatcagct cgacgagcac cgcgagctgg agctcgccgc gcccctgcac
     1441 atcccacgcg tccgggcgct cggtcggcag cacgcgcagc gagacgttcc cgacgagctc
     1501 ctgctcgagc cgcgccctga tcatgctcgc ggtcagccgg tcgccgtccc gtccggagag
     1561 cggcgaggtg ttgatcccga tcgtcatcga gaggctcggc tcgtcgacgc cgatcaccgg
     1621 cagcgggcgc gggtcgtcgg ggtcggcgag cgtctcgccg atcgtcacct gcggcatccc
     1681 ggcgacggcg atcagctccc ccgggccggc ctcctccgcg tcgacgcggt cgagcgcctc
     1741 ggtcacgtag agc
//
LOCUS       DASZOB010000402         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_169874, whole genome shotgun sequence.
ACCESSION   DASZOB010000402 DASZOB010000000
VERSION     DASZOB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169874"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..>1925)
                     /locus_tag="VG709_06395"
     CDS             complement(200..>1925)
                     /locus_tag="VG709_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWC32741.1"
                     /translation="NCLDRHVREGRADRRALVGVNERGERRDLTYGELLAEVEKVAAA
                     LRGMGVERGDRITIYMPTCVEAIALMLATVRIGAIHSVVFAGFGASALGERIAASGSR
                     LVFTADATYRKGKDVELLPIVDEAVEMAGEVVERVVVLSRGAGAATGATARHLSWQEF
                     VEAGEGHDPGFVPMEANEPAYILATSGTTAKPKLAVHTHGGYQGGIHAMGRICFGLRE
                     DEVWWSTSDIGWVVGHSYIVYAPLLAGCTTIAFEGALDHPGPEAFWKLVGELGVTGIF
                     TSPTAVRLLMRYGEEAARGADLSSLDRVFCAGEVLNAPAWEWLQKTVLEDRIPVVDHW
                     WQTETGGPVIGNPYGVELCPIKPGSAGFPLPGMDVAVMTPEGEPCGPNEKGILVFTRP
                     FPSLTPTLWGERERYGTDYWERIEGVYFTGDAVHVDEDGYVWFAGRADEVIKIAAHRL
                     GTIEVETAFLKHPAVAEAGVTGRPDEVRGEVISAFVVLKQGFEPSEELHGELLATVRR
                     ELGPVAVIGEIQFVDVLPKTRSGKIMRRVLKAVVLERDPGDITTIEDEGSVDEARQAV
                     EALRAGVR"
BASE COUNT          279 a          707 c          654 g          285 t
ORIGIN      
        1 tccggaatgc ggctcgcggc tggagaccgg cgccgcggta cgggagcacc aactcgacct
       61 cggcgtcgaa gccggctgat ttgtcgcctc cacgccccac gcacgaggac gcgtaggggc
      121 gaggcatgcc tggcccgcgc ccttctcgcg cagcggcgag agaagggtgc ggcgggtatg
      181 ccgcggccgc gggaccgcgt tagcgtacgc cggctctgag cgcctcgacc gcctgccggg
      241 cctcgtcgac cgagccctcg tcttcgatcg tcgtgatgtc gccgggatcg cgctcgagga
      301 ccacggcctt gaggacgcgc cgcatgatct tgccgctccg cgtcttcggc aggacgtcga
      361 cgaactggat ctcgccgatc actgcgacag ggccgagctc gcgtcgaacg gttgcgagca
      421 gctcgccgtg aagctcctcc gacggttcga atccctgctt gagcacgacg aaagccgaga
      481 tgacctcgcc gcgcacctcg tcgggacgcc cggtgacgcc ggcctccgcg accgcgggat
      541 gcttcaggaa cgcggtctcg acctcgatcg tcccgagtcg gtgcgcggca atcttgatca
      601 cctcgtccgc tcgcccggcg aaccagacgt agccgtcctc atcgacgtgg acggcgtcgc
      661 cggtgaagta gacgccctcg atccgctccc aatagtccgt gccgtagcgc tcgcgctcgc
      721 cccagagcgt cggcgtcagg ctcgggaacg gccgcgtgaa gaccaggatc cccttctcgt
      781 tcggcccgca cggctcgccc tcgggcgtca tgacggcgac gtccatgccc ggcagcggga
      841 agccggccga cccgggcttg atcgggcaaa gctcgacgcc gtacgggttg ccgatcacgg
      901 gcccgccggt ctcggtttgc caccagtggt ccacgaccgg gatgcgatct tcgagcaccg
      961 tcttctgcag ccactcccac gccggcgcgt tcagcacctc gccggcgcag aagacccggt
     1021 cgagtgagga gaggtcggcg ccgcgggcgg cctcctcgcc gtagcgcatg aggagccgaa
     1081 cggccgtggg cgacgtgaag atccccgtga cgccgagctc gccgacgagc ttccagaacg
     1141 cctcggggcc cggatggtcg agcgcgccct cgaacgcgat ggtcgtgcat ccggcgagga
     1201 ggggcgcgta cacgatgtag ctgtgcccca cgacccagcc gatgtccgag gtcgaccacc
     1261 agacctcgtc ctcgcggagg ccgaagcaga ttcggcccat ggcgtggatc ccgccctggt
     1321 agcccccgtg cgtgtgcacg gcgagcttcg gcttcgccgt cgtgccggac gtcgcgagga
     1381 tgtacgcggg ctcgttcgcc tccatcggca cgaacccggg atcgtggccc tcgccggcct
     1441 ccacgaactc ctgccaggac aggtgcctcg cggtggcccc cgtggccgcg ccggcgccac
     1501 gcgagaggac gacgacgcgc tcgaccacct cgcccgccat ctcgaccgcc tcgtccacga
     1561 tcggcagcag ctcgacgtcc tttccctttc ggtacgtcgc gtcggcggtg aagacgaggc
     1621 gcgacccgct cgccgcgatc cgttcgccaa gggcgctcgc gccgaacccc gcgaagacga
     1681 cggagtggat ggcgccgatg cgtacggtcg cgagcatgag cgcgatcgcc tcgacgcagg
     1741 tcggcatgta gatggtgata cggtcgccac gctcgacgcc catgcctcgc agcgcggccg
     1801 cgaccttctc gacctcggcg aggagctcgc cgtacgtgag gtcgcgccgc tcgccgcgct
     1861 cgttgacgcc caccagcgcg cgccgatccg cgcgtccttc ccggacatgg cggtcgaggc
     1921 agttg
//
LOCUS       DASZOB010000403         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_118828, whole genome shotgun sequence.
ACCESSION   DASZOB010000403 DASZOB010000000
VERSION     DASZOB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118828"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..307
                     /locus_tag="VG709_06400"
     CDS             89..307
                     /locus_tag="VG709_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWC32742.1"
                     /translation="MCRSIKTLRGTESASNDDDIRAAALQYVRKISGYRQPSRRNSEA
                     FDRAVDEISRASRELLEAVEQPRETARA"
     gene            315..821
                     /locus_tag="VG709_06405"
     CDS             315..821
                     /locus_tag="VG709_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HWC32743.1"
                     /translation="MRILPRSAWGAEPPRGPLRRHRIERVIVHESGHVVRDERDTLAN
                     LPAYQADHLARGWSDLAYHFVIDARGNVYRGRRPSTVGDTATDYDPRGSLLVMCDGAF
                     RWQRPGAEQVRALIHLLAWAQDTYGVAPQDIATHRDVAPIACPGARLHGLFADGSIGR
                     RVVRTRLV"
     gene            complement(832..1251)
                     /locus_tag="VG709_06410"
     CDS             complement(832..1251)
                     /locus_tag="VG709_06410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWC32744.1"
                     /translation="MDPTVPDGSDTTGGLASAVPFAAEIGIDLVSAAREEVRARLPWA
                     PERCTAGGVLHGGALMTLADTAGAVCAFLTLPPGATTSTIESKTNFFRTVRSGHVEAV
                     TRPLHVGRTTIVVQSDLFDADGRRVAQVTQTQSVREP"
     gene            1250..>1739
                     /locus_tag="VG709_06415"
     CDS             1250..>1739
                     /locus_tag="VG709_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWC32745.1"
                     /translation="MAKTRTMLSCAACGQPASQWVGRCPSCGAWGTIEEAARATAGAV
                     AVAQLSASPEDEPRIASGFGGVDRVLGGGVVRGSVVLVAGEPGIGKSTLLLQIAAKVG
                     TAERPALYASGEESQGQVASRARRLGVPADRISFVAGRDVHEVLDAGSSVRPPLLVVD
                     SIQ"
BASE COUNT          241 a          608 c          646 g          244 t
ORIGIN      
        1 ccgcggggac gcccgcgggc ggtaaggccg aggagcccgg cagccgcaag gttgccgggc
       61 tctcggcatc tcggcgtacc ctgggtgcat gtgccggagc atcaagacac tccgcgggac
      121 cgaatccgcc tcgaacgacg acgacatccg cgccgcggcg ctgcagtacg tccggaagat
      181 cagcgggtac cgacagccgt cgcgacggaa ctccgaggcg ttcgaccggg ccgtcgacga
      241 gatctcgcgc gcgtcgcggg agctcctcga ggcggtcgag cagcctcgcg agacggcgcg
      301 cgcctgacac ccgcttgagg atccttccgc ggagcgcctg gggcgctgag ccgccgcgag
      361 ggccgctaag gcgccaccgg atcgagcgcg tcatcgtcca tgagagcggc cacgtcgtcc
      421 gcgacgagcg agacacgctc gcgaaccttc ccgcgtacca ggcggaccat ctggcgcggg
      481 gctggtcgga ccttgcctat cacttcgtga tcgacgcgcg cgggaacgtc taccggggac
      541 gacgcccgtc gacggtcggc gacacggcaa cggattacga ccctcgcggg agtctgctcg
      601 tgatgtgcga tggcgccttc cggtggcagc gtcccggcgc cgagcaggtc cgcgcgctca
      661 tccacctcct cgcgtgggcc caggacacgt acggcgtcgc gccccaggac atcgcgacgc
      721 accgagatgt cgccccgatc gcgtgcccgg gggcgcgcct ccacgggctt ttcgcggacg
      781 gatcgatcgg acgccgagtc gtacgtactc gcttggtctg agaagagccg gtcagggctc
      841 gcgcaccgat tgagtctgcg tgacttgagc gacccgcctg ccgtcggcgt cgaagaggtc
      901 gctctgaacg acgatcgtcg ttcgaccgac gtgcagtggg cgcgtcaccg cctctacgtg
      961 tccggaccgg acggtcctga agaagttggt cttcgactcg atcgtgctgg tcgtcgctcc
     1021 gggcggtagg gtgaggaacg cgcacacggc gccggccgtg tcggcgagcg tcatcagcgc
     1081 cccgccgtgg agcacgccgc cggccgtgca ccgctcgggg gcccacggga gccgcgcgcg
     1141 gacctcttcg cgggcggcgg agacgagatc gatgccgatc tcggcagcga acgggaccgc
     1201 cgaggcgagc ccgccggtcg tgtcagaccc gtcgggtacg gtcggatcca tggcgaagac
     1261 gcggaccatg ctctcatgcg cggcctgcgg gcagccggcc tcgcagtggg tcggcagatg
     1321 tccttcgtgc ggcgcctggg ggacgatcga ggaggcggcc cgcgcgaccg cgggcgcggt
     1381 cgccgtcgca cagctgtccg cctcgccgga ggacgagccg cggatcgcga gcggcttcgg
     1441 cggcgtcgac cgcgtgctcg gcggcggcgt cgttcgaggg tcggtcgtgc tggtggccgg
     1501 cgaaccgggc atcgggaagt cgacgctgct cctccagatc gccgcgaaag tcggcacggc
     1561 ggagcgaccc gcgctctacg cgtccggaga ggagtcgcag ggacaggtcg cctcgcgcgc
     1621 tcggcggctc ggcgtcccgg ccgaccgcat ctcgttcgtc gcgggacggg acgtccacga
     1681 ggtcctcgac gcgggctcgt cggtccgccc ccccttgctc gtcgtggact cgatccagg
//
LOCUS       DASZOB010000404         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_89341, whole genome shotgun sequence.
ACCESSION   DASZOB010000404 DASZOB010000000
VERSION     DASZOB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89341"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VG709_06420"
     CDS             complement(<1..458)
                     /locus_tag="VG709_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32746.1"
                     /translation="MAEPPREDRDRRTDEDLFEDLDQFFAPLDDDWPEDEGAEPPGRA
                     VDARSEPPPAAAEPEPEPAAEPPSADAAPADDLVDATAEMGPEWDLSDELPEAEPAAP
                     SSQPPRPPAATTDEEDLNAADLRAPPPAYARLPGPRAEPRPADEPAARPE"
     gene            complement(458..1018)
                     /gene="frr"
                     /locus_tag="VG709_06425"
     CDS             complement(458..1018)
                     /gene="frr"
                     /locus_tag="VG709_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWC32747.1"
                     /translation="MTAEETLRDATHKMARAVEVTREELSTVRTGRATPAILNRVNVD
                     YYGSHVPLNQLASFSVPEPRLLVIQPFDRNAISAIERAIMQSDLGLTPSNDGNLIRLA
                     FPSLTEERRKELIRMVRERGEEGRVAIRNVRRHAKEELERLERSGSISEDDLARSERE
                     LQKQTDKYVAEVDGLLGHKEKELMEV"
     gene            complement(1015..1764)
                     /gene="pyrH"
                     /locus_tag="VG709_06430"
     CDS             complement(1015..1764)
                     /gene="pyrH"
                     /locus_tag="VG709_06430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWC32748.1"
                     /translation="MSDGGEPLAYRRVLLKLSGDAFAPPGGEFGIYPESVELLASQLA
                     DVLSMGVQPAVVVGGGNIWRGRDAGPMDRARADYVGMLATVMNALVLQDALERIGVVT
                     RVQTAIAMAQIAEPYIPRKAMRHLEKGRVAIFAAGLGRPFFSTDTTAAQIALEIHAEA
                     ILKGTRVDGVYDSDPNLNPDAVKFDDLEYIEVLKRGLQVMDATAISLAMDNDLPIIVF
                     DYREPGNIRRATQGERIGTLVHATSPERRVS"
     gene            complement(1761..2369)
                     /gene="tsf"
                     /locus_tag="VG709_06435"
     CDS             complement(1761..2369)
                     /gene="tsf"
                     /locus_tag="VG709_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWC32749.1"
                     /translation="MAITSGDVKRLRDETGAGMMDCKRALEEAGGDFEQAREILRKKG
                     LADVAKRSGKIAAEGVVEAYIQGSGKRGALVEVNCETDFVANTPEFRTLAREIAMQII
                     AAEPEWVAREDVPEDVVERERKLFEEQFREQGKPENILPKIVEGKLGDFYEQRCLLDQ
                     PFFKDSDGKRTVGDRVAEVSAKVGEKIEVRRFAHFVRGEAGS"
BASE COUNT          311 a          913 c          834 g          422 t
ORIGIN      
        1 tcctccggcc gcgccgcggg ctcgtcggcg gggcgtggtt cggcgcgcgg ccccggaagc
       61 ctcgcgtacg cgggcggcgg cgcccggaga tccgcggcgt tcagatcttc ctcgtccgtc
      121 gtggccgcgg gcggtcgtgg cggctgcgac gatggcgcag cgggttccgc ctccggcagc
      181 tcgtccgaga ggtcccactc cggacccatc tctgcggtcg catcgaccag gtcgtcggcg
      241 ggggcggcgt cagcggacgg cggttcggcc gccggttcgg gctccggctc ggcagcggcc
      301 ggcggcggct cggaacgcgc gtcgaccgca cgaccgggtg gctcggcgcc ttcgtcctcg
      361 ggccagtcgt catcgagcgg agcgaagaac tgatcgagat cctcgaagag gtcctcgtcg
      421 gtcctccggt cccggtcctc ccgcggaggc tcggccatca gacctccatc agctccttct
      481 ccttgtggcc gagcagcccg tcgacctcgg ccacgtactt gtcggtctgc ttctgcagct
      541 ctcgctcgga ccgggcgagg tcatcttcgg agatggaacc agagcgttca agcctttcga
      601 gctcctcctt cgcgtggcgg cgcacgttgc ggatcgcgac gcgcccctcc tcgccgcgct
      661 cgcgcaccat ccggatcagc tccttgcggc gctcctcggt caacgacggg aaggcgaggc
      721 ggatcaggtt gccgtcgttg ctcggcgtga ggcctagatc ggactgcatg atcgcgcgct
      781 cgatcgccga gatcgcgttg cggtcgaacg gctggatcac gagcagccgc ggctccggca
      841 ccgagaacga cgcgagctgg ttcagcggga cgtggctgcc gtagtagtcg acgttcacgc
      901 ggttcaggat cgccggggtc gctcggcccg tccggaccgt cgagagctcc tcgcgcgtca
      961 cctcgaccgc gcgcgccatc ttgtgcgtcg cgtcgcggag cgtctcctcg gccgtcacga
     1021 caccctcctc tccggcgacg tcgcgtggac gagcgtcccg atcctctcgc cctgggtcgc
     1081 gcgccggatg ttcccgggct cgcggtagtc gaacacgatg atcggcaggt cgttgtccat
     1141 cgcgagcgag atcgccgttg cgtccatgac ctgcagcccg cgcttgagca cctcgatgta
     1201 ctcgaggtcg tcgaacttca cggcgtcggg gttgaggttc gggtccgagt cgtacacgcc
     1261 gtcgacgcgc gtgcccttga ggatcgcctc ggcgtggatc tcgagcgcga tctgagcggc
     1321 cgtcgtgtcg gtagagaaga acgggcggcc gaggcccgcc gcgaagatcg cgacgcggcc
     1381 cttctcgagg tgacgcatcg ccttccgcgg gatgtacggc tccgcgatct gcgccatcgc
     1441 gatcgcggtc tggacgcgcg tcacgacccc tatacgctcg agggcgtcct gtagcaccag
     1501 cgcgttcatc acggtcgcga gcatcccgac gtagtcggcg cgcgcccggt ccatcggccc
     1561 ggcgtcgcgc ccgcgccaga tgttgccgcc gccgacgacg acggccggct ggacgcccat
     1621 cgacaagacg tcggcgagct ggctcgcgag cagctcgacc gactccggat agatcccgaa
     1681 ctctccgccg ggcggcgcga acgcatcgcc cgagagcttc aacaggacgc ggcggtacgc
     1741 gagcggctcg ccgccgtcgc tcacgagcca gcctcgcccc tgacgaagtg cgcgaaccgg
     1801 cggacctcaa tcttctcgcc gaccttggcg ctcacctcag cgacgcggtc gccgaccgtg
     1861 cgcttgccgt ccgagtcctt gaagaacggc tggtcgagga ggcaccgctg ctcgtagaag
     1921 tcgcccagct tgccctcgac gatcttcgga aggatgttct ctggcttccc ctgctcgcgg
     1981 aactgctcct cgaacagctt cctctcgcgc tcgacgacgt cctcggggac gtcctcgcgc
     2041 gcgacccact cgggctccgc ggcgatgatc tgcatcgcga tctcgcgcgc gagcgtccga
     2101 aactcggggg tgttggcgac gaagtccgtc tcgcagttga cctcgacgag tgcgccgcgc
     2161 ttcccactac cctggatgta ggcctcgacg acgccctccg cggcgatctt ccccgagcgc
     2221 ttcgcgacgt cggcgagccc cttcttccgc aggatctccc tcgcctgctc gaagtcgccg
     2281 ccggcctcct cgagcgcacg cttgcagtcc atcatccccg cgcccgtctc gtcgcggagc
     2341 cgcttgacgt cgccggaggt gatcgccatg ggtccaggcg attctgccat ctaggccgtc
     2401 ccccctgtgg cgtcggcctg ctccgcgggt cgtgcggccg gcgcctccgc cgccgctccc
     2461 ggatccgcgg cggccccggc
//
LOCUS       DASZOB010000405         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29115, whole genome shotgun sequence.
ACCESSION   DASZOB010000405 DASZOB010000000
VERSION     DASZOB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29115"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="hflX"
                     /locus_tag="VG709_06440"
     CDS             <1..902
                     /gene="hflX"
                     /locus_tag="VG709_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWC32750.1"
                     /translation="YNLPRMRGMWQHLERLGGGIGTRGPGETQLETDRRLARRRIALL
                     KRRLRELERRRETQRKARRRGETPVVALAGYTNAGKSTLLNALTDADASVDDRLFETL
                     DPTTRAFVHEGRRYLVTDTVGLIRRLPHQLVEGFAATLEETLSADLVLHVADASQPDG
                     LLDDTIAAVEGVLSELGGGGLPVELVLNKIDGADPLRRRRLANRFPGAHQISALTGDG
                     LEGVKRGVARRFADRFHPVRLLVPYDDGNVLARLYELGAPIDDRDDTEDGVLLVARLP
                     RDEVARFGRYVVAESAPARARGR"
     gene            914..1336
                     /gene="dut"
                     /locus_tag="VG709_06445"
     CDS             914..1336
                     /gene="dut"
                     /locus_tag="VG709_06445"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWC32751.1"
                     /translation="MRRVRADARMPERAYAGDAGLDLAACDRLELAPGERAVVRTGIA
                     VAIPAGYAGFVQPRSGLAARHGIAVVNSPGLIDSGYRGEINVVLLNTDRSRPFVAEAG
                     ERVAQLVVLAVPDVAVREVEDLPESERGARGFGSSHVT"
     gene            1333..>1535
                     /locus_tag="VG709_06450"
     CDS             1333..>1535
                     /locus_tag="VG709_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWC32752.1"
                     /translation="MINEPRIRVAAMLRREGRILLCRQEKNGREYWLLPGGGVNAGET
                     LVDAVQRELGEECAIDEDLPLEGP"
BASE COUNT          210 a          546 c          567 g          212 t
ORIGIN      
        1 agtacaacct cccgcgcatg cgcgggatgt ggcagcacct cgagcggctg ggcggcggaa
       61 tcgggacgcg cggccccggc gagacgcagc tcgagacgga ccgccggctc gcccgccgga
      121 ggatcgcgct gctcaagcga cggctgcgcg agctcgagcg gcggcgcgag acgcagcgga
      181 aggcgcgccg gcggggcgag acccccgtcg tcgcgctcgc gggctacacg aacgcgggca
      241 agtcgacgct cttgaacgcg ctgaccgatg ccgacgcctc ggtcgacgat cgcctgttcg
      301 agacgctcga cccgaccacg cgcgccttcg tgcacgaggg tcgccgctac ctcgtcacgg
      361 ataccgtcgg gctcatccgg cggcttccgc accagctcgt cgaggggttc gcggcgacgc
      421 tcgaggagac gctctccgcc gacctcgtcc tccacgtcgc ggacgcgtcg cagcccgatg
      481 ggctccttga cgacacgatt gcggcagtcg aaggcgtcct gagcgagctg ggcggcggcg
      541 gactgcccgt cgagctcgtg ctcaacaaga tcgacggcgc cgatccgctc cgtcggcgcc
      601 ggctcgcaaa ccggtttccc ggagcgcacc agatctcggc gttgaccggc gacggcctcg
      661 agggagtgaa gcgcggcgtt gcgcgacgct tcgccgaccg cttccacccg gtccggctgc
      721 tcgtgccgta cgacgacggc aacgtgctcg cgcggctgta cgagctcggc gctccgatcg
      781 acgaccgcga cgacaccgag gatggcgtcc tgctcgtcgc ccggcttccg cgtgacgagg
      841 tcgcgcgctt cggtcggtac gtggtcgccg agagcgcacc cgcccgcgcc cgcggccggt
      901 gatcgaggtt gagatgcggc gtgtgcgcgc cgacgcacgc atgccggagc gtgcctacgc
      961 cggcgacgcc gggctcgatc tggccgcctg cgatcggctg gagctcgctc ccggagaacg
     1021 ggccgtcgtc cggacgggca tcgcggtggc gatcccggcg ggctacgccg gcttcgtcca
     1081 gccgcgctcg gggctcgccg cccggcacgg catcgcggtc gtcaactcac ccgggctgat
     1141 cgactcgggt taccgcggag agatcaacgt cgtgctcctg aacacggacc gctcgcggcc
     1201 gttcgtcgcc gaggcgggcg agcgcgtcgc tcagctcgtg gtgctcgccg tcccggacgt
     1261 cgcagtccgc gaggtcgaag acctgcccga gagcgagcgt ggcgcgcgcg ggttcggttc
     1321 ctcccacgtc acgtgatcaa cgagccgcgg atccgggtgg cggcgatgct ccgacgcgag
     1381 ggccggatcc tgctctgccg gcaggagaag aacggacgcg agtactggct cttgcctggg
     1441 ggcggcgtga acgctggcga gacgctcgtc gacgccgtcc agcgtgagtt gggcgaagag
     1501 tgcgcgatcg acgaggacct gccgctcgag gggcc
//
LOCUS       DASZOB010000406         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_175789, whole genome shotgun sequence.
ACCESSION   DASZOB010000406 DASZOB010000000
VERSION     DASZOB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175789"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1130
                     /locus_tag="VG709_06455"
     CDS             48..1130
                     /locus_tag="VG709_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HWC32753.1"
                     /translation="MTGSFPNISPRRRLVAVALVVLASTAAVVGIARPAEALEGTFFP
                     TVSRGNRGEDVVTAQLLLRRRGYDTPTRGLMNADMVAAVRKFQADRGLPVTGVIGTRT
                     WVKLVVRAVRGQQGPHVLALQRQLARKHGYDVARDGVFGRKTEEAVIEFKRHMGLEPN
                     GIVARGAWRNLLWHYQPVRDDRKTCVLDRSAAWGMATTVGALRRAAIRFFRTGNGGVA
                     TGHLSYTHGGPISPHVSHQEGMDADLRPIRDDNAQCSAPVRWDDEKYDRKATRTLIEE
                     LYRASGNKIVVIWFNDPRLVREGLTESLSGHDGHMHVRWCLETPPDDHYNCDNRSWYR
                     GPSAATHDGLEEDGTEAGLYGPYEDG"
     gene            complement(1115..>1828)
                     /locus_tag="VG709_06460"
     CDS             complement(1115..>1828)
                     /locus_tag="VG709_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWC32754.1"
                     /translation="DKQRRREEAWALLERRGVARFPGARGRIPNFVGADAAAERLAAL
                     PEWGPARVVKANPDAPQLPVRARALADGKRLYMAVPRLRSERPFVLLDAARLAVPPRR
                     AASIRGAAAAGRTVPIEQMERIDLVVCGSVAVDRRRARLGKGGGYSDIELALLVEAGF
                     VDGETTLVATVHPLQVVDGPLPETDHDFRVDAVVTPERTLHCRRRRRPPGILWDHLDP
                     AKIEAVPALRRAAGAQPSS"
BASE COUNT          264 a          672 c          655 g          237 t
ORIGIN      
        1 cccgcgggtc ccgccctcac cgtttcccat agcgatccga ggcgcatgtg accggaagct
       61 tccccaacat ctccccgcgc cgccggctcg tcgccgtcgc gctcgtcgtg ctggcgtcga
      121 ccgccgcggt ggtcgggatc gcgcgtcccg ccgaggcgct cgagggcacg ttcttcccga
      181 cggtgtctcg cgggaaccgc ggtgaggacg tcgtcaccgc gcagctgctc cttcgacgcc
      241 gcggctacga cacgccgacg agggggctga tgaacgccga catggtcgcg gccgtccgga
      301 agttccaggc ggatcggggc cttccggtga ccggcgtgat cggaacccgg acgtgggtga
      361 agctcgtcgt gcgcgccgtc cgcgggcaac agggcccgca cgtcctcgcc ctccagcgac
      421 agctcgcgcg caagcacggg tacgacgtgg cgcgcgacgg cgtcttcgga cgcaagacgg
      481 aggaggccgt gatcgagttc aagcgccaca tgggcctcga gccgaacggg atcgtcgcgc
      541 gcggcgcgtg gcgcaacctg ctctggcact accagcccgt ccgcgacgat cggaagacgt
      601 gcgtcctcga tcgctcggcg gcgtggggga tggcgacgac cgtcggagcg ctgcggcgcg
      661 ccgcgatccg gttcttccgc accgggaacg ggggcgtcgc gaccggccac ctcagctaca
      721 cgcacggcgg tccgatctct ccgcacgtca gccaccagga gggcatggac gccgacctcc
      781 gtccgatccg cgacgacaac gcccagtgct cggcgccggt tcgctgggac gacgagaagt
      841 acgaccggaa ggcgacgcgg acgctcatcg aggagctgta ccgggcctcc gggaacaaga
      901 tcgtggtgat ctggttcaac gatccccgcc tggtccgtga gggcctgacc gagagcctgt
      961 cgggccacga cggccacatg cacgtccggt ggtgcctcga gacgcccccg gacgaccact
     1021 acaactgcga caaccggagc tggtaccggg gtccgagcgc ggccacgcac gacgggctcg
     1081 aggaggacgg gaccgaggcg ggcctctacg gcccctacga ggacggctga gcgcccgcgg
     1141 ctcgtcgcag ggccggcacc gcctcgatct tcgcgggatc gaggtgatcc cagaggatcc
     1201 ccggtggacg acggcgccgg cgacagtgca acgtgcgctc cggtgtgacg accgcatcga
     1261 cgcggaagtc gtgatcggtc tccggcagcg gcccgtccac gacctggagc gggtgaacgg
     1321 tcgccacgag cgtcgtctcg ccgtcgacga acccggcctc gacgaggagc gcgagttcga
     1381 tgtccgagta gccgccgccc ttcccgagcc gagctctccg gcgatccacc gcgacgctcc
     1441 cgcagacgac gagatcgatc cgttccatct gctcgatcgg gaccgtcctg cccgccgcgg
     1501 ccgctcctcg gatcgacgcc gcgcgccggg gcggcaccgc gagccgcgcc gcgtcgagga
     1561 gcacgaacgg gcgctcggag cggaggcgcg gcaccgccat gtacaggcgc ttgccgtcgg
     1621 cgagcgcgcg tgcccggacc ggaagctgcg gcgcgtccgg gttcgccttt acgacgcggg
     1681 ccggccccca ttcgggcagc gcggcgagcc gctcggccgc ggcgtcggct ccgacgaagt
     1741 tcgggatccg tccccgggcg ccggggaacc gcgcgacgcc gcgacgctcg aggagcgccc
     1801 acgcctcctc gcgcctccgc tgcttgtc
//
LOCUS       DASZOB010000407         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_129524, whole genome shotgun sequence.
ACCESSION   DASZOB010000407 DASZOB010000000
VERSION     DASZOB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129524"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1486)
                     /locus_tag="VG709_06465"
     CDS             complement(74..1486)
                     /locus_tag="VG709_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWC32755.1"
                     /translation="MDGLAALRSRLPEGVVSTHPGETQSRSRDWWPLAMLREARGEIP
                     TRPAAVVFPRTTEAVAEVLAWADETGTAVVPRGAGSGVCGGAQALRGAVVVDVSRMDR
                     IISVDEESACVEVQAGIRGTRLEEGLEPYGLTTGHYPQSLAISSVGGWIAARSAGQAS
                     AGYGVIEDLVLGLVVVLAGGEVVTLRSLPRSAAGPDLRELFLGSEGTLGIVTEATLVA
                     SRRPVGLRWLAFRPRGFDEAIVLAREVERSGARLLVLRAYDDADAAVTFGGLDHPGGP
                     VMIVAAARDRWLDATLEAVRDVARERGADPLPDEYGRHWWEHRFDAVRLYRRVMGEER
                     LLGHGVVVDTMEVAGLWSRLGRLYAEVSGALSRHAEAVGCHLSHPYRSGASLYFTFLL
                     RAPDDARAEEPYRACWTDAAAACHAAGGTVTHHHGVGILKAPFLEEELGTPGIGLLRR
                     VKDALDPRGVLNPGKLLPPR"
BASE COUNT          224 a          604 c          564 g          192 t
ORIGIN      
        1 agctcgcgcc cgacgcggcc gctccccgat ctcgggttga cgatcagcac gacgggtccg
       61 aacgggctcg ccatcagcgc ggcgggagca gcttgccggg gttcaggacg ccgcgtggat
      121 cgagcgcgtc cttcacgcgg cgaagcagcc cgatccccgg tgttccgagc tcctcttcga
      181 ggaacggcgc cttcaggatc ccgacgccgt ggtggtgggt gacggtcccg ccggcggcgt
      241 ggcaggcggc cgcggcgtcg gtccagcagg cgcggtaggg ctcctccgcc cttgcgtcgt
      301 ccggcgcgcg gagcaggaac gtgaagtaga gggacgcgcc cgagcggtac gggtgcgaga
      361 ggtgacatcc aacagcctcg gcatggcggc tcagcgcccc gctcacctcg gcgtagaggc
      421 gcccgagccg cgaccacagt ccggccacct ccatcgtgtc gaccacgacg ccgtgcccga
      481 ggaggcgttc ctctcccatc actcgccggt agagccgaac cgcgtcgaag cggtgctccc
      541 accagtggcg cccgtactcg tccggcagcg ggtcggctcc ccgctcacgc gcgacgtcgc
      601 gcacggcctc gagcgtcgcg tcgagccagc ggtcgcgcgc ggccgcgacg atcatcacgg
      661 gcccgccggg atggtcgagg ccgccgaacg tcacggcggc gtccgcgtcg tcgtacgcgc
      721 ggagcacgag caggcgcgca cccgaccgct cgacctcgcg cgcgagcacg atcgcctcgt
      781 cgaagccgcg cggacggaac gccagccacc ggaggcccac cgggcgccgt gacgccacga
      841 gcgtcgcctc ggtcacgata ccgagcgtcc cttcggagcc gaggaagagc tctcggaggt
      901 ccggccccgc cgcggagcgc ggcagcgatc gcagcgtgac cacctcgcct cccgccagca
      961 cgacgacgag cccgagcacc aggtcctcga tcacgccgta gcccgcggac gcctgaccgg
     1021 ccgaccgcgc tgcgatccac cctccaaccg acgagatcgc gagtgactgc gggtagtgcc
     1081 ccgtcgtgag gccgtagggt tccaggccct cctcgagccg cgtgccccgg atgccggcct
     1141 gcacctcgac gcacgcggac tcctcgtcga cggagatgat gcggtccatc cgcgagacgt
     1201 cgacgacgac ggcgcctcgg agggcctggg caccgccaca gacgcccgac ccggctcccc
     1261 gggggacgac cgcggttccc gtctcgtccg cccacgcgag gacctcggcg acggcctcgg
     1321 ttgtgcgggg aaagacgacg gccgccggcc tcgtcgggat ctcgccgcgc gcctcgcgaa
     1381 gcatcgcgag cggccaccag tcgcgggagc gggactgggt ctctcccggg tgggtgctca
     1441 cgacgccttc ggggaggcgt gacctcagcg ccgcgagccc gtccacgcgc cgatgatagt
     1501 gacccgggcg ggctcgagac tcagacgccc gtgtaggcga cgacccctcg gttcacgctg
     1561 gtccgtgccg cggccgcatc gggg
//
LOCUS       DASZOB010000408         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_149801, whole genome shotgun sequence.
ACCESSION   DASZOB010000408 DASZOB010000000
VERSION     DASZOB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149801"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VG709_06470"
     CDS             complement(<1..1194)
                     /locus_tag="VG709_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HWC32756.1"
                     /translation="MDETSWPRPSRLSVAAAGHGIPRKGAIHWPRCAPSRRDRARPLG
                     AALITATNGKTTTAAMVAEILGPRTTLAHNRAGANLVSGVASALLEADDAELGLFEVD
                     EAAFPEVARLTRPRVVCLANLFRDQLDRYGELELIAERWRTAGAELDPAAAAVVNADD
                     PLIANLVRGRQHAVRFGLDAPGVARERLPHAADSKYCVECGTPYEYDAAYVGHLGAYR
                     CPRCGNTRPPLDVVARSIQLEGLERSSFDLVSPAGVRRIEMGLPGLYNVYNALAAASL
                     SLVLGASLDEIADGLGRFRAAFGRFERIQVGERTVLVLLVKNPAGANEAVRTLVDGSA
                     PSRAVVALNDEIADGRDVSWIWDVDFEPLLDCLERVVVTGARAHELALRFKYGGLDEE
                     RIEIVP"
     gene            1318..>2463
                     /locus_tag="VG709_06475"
     CDS             1318..>2463
                     /locus_tag="VG709_06475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWC32757.1"
                     /translation="MSPPARGRRRDDGHLPARAALFLVGVAVATAAVAAEPLSELRSG
                     TGGWVTFFVLAAAAAVAQLFAVQAPRNAARHYTTVVFLIAGVLLLPAGLVALLGVVQH
                     VPEWVKLRYAWYIQTFNILNYTLSCLAAWWAVHLVLDPVDGLSAGGRWAAAAAVACLA
                     FVSLNHGLLAPMMHLAGRRRIWKSELFSIESLSTDLILAALGAGFATLWLTNRWLVLA
                     VVAPLVLIQRSLNVAGLEEEARVDAKTGLYNARHFAAEVKDELERSQRLGRPMSVIMA
                     DLDLLRDINNSYGHLAGDAVLRGVADVFRRQLRDSDVAARFGGEEFAVLLPETDGDEA
                     MDIAERIRLTTAETPIWAESAGQAINVTMSVGVASFPTHGTDPDDLLH"
BASE COUNT          347 a          888 c          875 g          353 t
ORIGIN      
        1 gggcacgatc tcgattcgct cctcgtccag gccgccgtac ttgaagcgaa gcgcgagctc
       61 gtgcgcgcgc gccccggtga cgacgacacg ctcgagacag tcgaggagcg gctcgaagtc
      121 gacgtcccag atccacgaga cgtcgcggcc gtcggcgatc tcgtcgttca acgcgaccac
      181 cgcccgcgaa ggagccgatc cgtccacgag ggtccgtact gcctcgttcg ccccggccgg
      241 gttcttgacg agcaggacga gcacggtgcg ctcgcccacc tggatccgct cgaagcggcc
      301 gaacgcggcg cgaaaacggc cgagcccgtc cgcgatctcg tcaaggctcg ccccgaggac
      361 gagcgagagc gacgccgccg cgagcgcgtt gtagacgttg tagagcccag gcaggcccat
      421 ctcgatccgc cggacgccgg cgggagagac gaggtcgaag ctcgagcgct cgagcccctc
      481 gagctggatg gaccgcgcga cgacgtcgag cggcggcctc gtgttgccgc agcggggaca
      541 gcggtaggcg ccgaggtgcc cgacgtaggc ggcgtcgtac tcgtagggcg ttccgcactc
      601 gacgcagtac ttcgagtccg cggcgtgggg gaggcgctcg cgcgcgacgc ccggggcgtc
      661 gagcccgaac cgaacggcgt gctgccgccc gcgcacgagg ttcgcgatga gcgggtcgtc
      721 ggcgttgacg acggccgcgg ccgcggggtc gagctccgcc ccggcggtac gccaccgctc
      781 ggcgatgagc tccagctcgc cgtagcggtc gagctggtcg cggaagaggt tggcgaggca
      841 gacgacccga ggacgcgtga gccgcgcgac ctcagggaag gcggcctcgt cgacctcgaa
      901 cagccccagc tcggcgtcgt cggcctcgag cagggcggaa gcgacgcccg atacgaggtt
      961 cgcgccggcg cggttgtgcg cgagggtcgt acgcggtccg aggatctccg cgaccatggc
     1021 cgccgtcgtc gtcttcccgt tggtggcggt gatgagcgcc gcgccgagcg ggcgagcgcg
     1081 atctcggcgc gaaggcgcac aacggggcca gtgtattgct ccctttcgag ggattccgtg
     1141 gccggcggcc gcgaccgaca accgggatgg gcgtggccac gaggtctcgt ccaccgctcg
     1201 ttcaccaagc cgcccgcgag gacgcgcgga ggaggaatga agaccccgaa ctggatcgct
     1261 gcggcagccg gacgcgcctc acgaagtgga gcgacttccg ccttgtcgcg cggagcgatg
     1321 tcccccccgg cgcgcggccg gcggcgcgac gacggacatc tcccggcgcg ggccgcgctc
     1381 ttcctcgtcg gcgtagccgt cgcgacggca gcggtcgcgg ccgagccgct ctcggagctc
     1441 aggtcgggca ccggcggatg ggtgacgttc ttcgtcctcg ccgccgccgc cgccgtcgct
     1501 cagctcttcg cggtgcaggc gccgcggaac gccgcccgcc actacacgac ggtggtcttc
     1561 ctgatcgccg gcgtcctcct gcttcccgcc ggcctcgtcg ctctcctcgg cgtcgtccag
     1621 cacgtccccg agtgggtcaa gctccgctac gcctggtaca tccagacgtt caacatcctc
     1681 aactacacgt tgagctgtct ggccgcgtgg tgggcggtgc acctcgtcct cgaccccgtg
     1741 gacggactga gtgccggcgg ccggtgggcg gctgcggccg ccgtcgcctg cctcgccttc
     1801 gtctcgctga accacggcct cctggcgccg atgatgcacc tcgccggtcg ccggcggatc
     1861 tggaagagcg agctcttctc gatcgagagc ctctcgaccg acctgatcct ggcagcgctc
     1921 ggcgccggct tcgcgacgct gtggctcacc aaccgctggc tggtgctcgc ggtcgtggcg
     1981 cccctcgtgc tgatccagcg ctcgctgaac gtcgcaggtc tcgaggagga ggcgcgcgtc
     2041 gacgcgaaga cgggtctcta caacgcgcgc cacttcgccg ccgaggtgaa ggacgagctg
     2101 gagcgctcac agcgcctcgg ccgccccatg tcggtgatca tggccgacct cgacctcctc
     2161 agggacatca acaactcgta cggccacctc gcgggcgacg ccgtcctgcg cggtgtcgcg
     2221 gacgtcttcc ggcgccagct ccgcgactcc gacgtcgcgg cccgcttcgg cggggaggag
     2281 ttcgcggtcc tgctccccga aaccgacggc gacgaggcga tggacatcgc cgagcgcatc
     2341 cgcttgacga cggcggagac tccgatctgg gcggagagcg cgggacaggc gatcaacgtc
     2401 acgatgtccg tcggcgtcgc gtcgttcccg acgcacggca ccgacccgga cgacctcctc
     2461 cac
//
LOCUS       DASZOB010000409         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_160141, whole genome shotgun sequence.
ACCESSION   DASZOB010000409 DASZOB010000000
VERSION     DASZOB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160141"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..667)
                     /locus_tag="VG709_06480"
     CDS             complement(131..667)
                     /locus_tag="VG709_06480"
                     /inference="COORDINATES: protein motif:HMM:NF022798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3156 family protein"
                     /protein_id="HWC32758.1"
                     /translation="MWGRARAAAAQALTRNVEVFERDGYRLTAREGFLSARLEADDGR
                     ALRLRMAQAGRVFGGSYALEIAPVDPVLPATGGLTARGRGVVRLSRVAFRSRRGDAAG
                     RRLAERLDADPALQRALADVHFERVRVDSEGRPVVRHMGGSVVWALFPPVIRPVPLVP
                     EQARATVRALEAFTSVRM"
     gene            complement(670..>1824)
                     /locus_tag="VG709_06485"
     CDS             complement(670..>1824)
                     /locus_tag="VG709_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWC32759.1"
                     /translation="APVWWYEWPFGRGFWCVTKHADVVAVSRDTKTYSSAQGANLEDL
                     DEDARDARQSMLETDPPRHTRLRGLVGPPFTPRAVRAYENALRELTREILDRALLRRE
                     FDFVEEIAKELPIRVLARLLGVPDEDNARLIDWGDRMIANTDPDFADVLHDSPASDRY
                     RLLPFRSPAALELFEYGHRIAAERRARPRDDLVSKLVNAEIDGERLSEREFDTMFLLL
                     VVAGNETTRQAIAHGMLALMEHRDEWGRMRDDPALAWETGADEILRWSTPVLHFRRTA
                     TADVELRGQQIRTGDKVVVWYASANFDEEVFDEPLRFDVGRRPNRHLTFGGGGPHYCL
                     GAYLAKLEVQVMFDELLPRLADMELAGSVRRVRTNFTNALKAMPVRITSA"
BASE COUNT          263 a          657 c          652 g          252 t
ORIGIN      
        1 cctcgcgact cagcgtatcg acgcgactct caccggtggg tcacggttcc ccacgaaacc
       61 atccgacgtg tgcgccggaa ccgtcgcacg tactcgcagc ggcctcgcct gcgccgtcgt
      121 ccgacgctcg ctacatacga accgacgtga acgcctcgag cgcccggacg gtcgcgcgcg
      181 cctgctcggg gacgagcggc accggcctga tcacgggcgg gaagagggcc cacacgaccg
      241 agccgcccat gtggcggacg accggccttc cctcggaatc gacgcgcacg cgctcgaagt
      301 ggacatcggc gagcgcccgc tggagcgccg gatcggcgtc caggcgctcc gcgaggcggc
      361 gaccggccgc gtcgccgcgc cgtgaacgga acgcgacgcg gctgaggcgg acgacgcctc
      421 gtccgcgcgc cgtcaggccg ccggtcgccg gcagcaccgg gtcgacgggt gcgatctcga
      481 gcgcgtagct tcctccgaag acgcggccgg cctgagccat gcgaaggcgg agcgctcggc
      541 cgtcgtcggc ttccaggcgc gcagaaagga agccttctcg tgccgtcagc cggtacccgt
      601 cccgctcgaa gacctcgacg ttgcgagtga gcgcctgcgc cgccgccgcc cgtgctcgac
      661 cccacatcgc tacgccgacg tgatccgcac gggcatcgcc ttgagcgcgt tcgtgaagtt
      721 cgttcgcacg cgccggaccg acccggcaag ctccatgtcg gcgaggcgcg gaagcagctc
      781 gtcgaacatc acctgcacct cgagcttcgc gaggtaggcg ccgaggcagt agtgcggccc
      841 gccgccgccg aaggtgaggt ggcggttcgg ccgccgcccg acgtcgaacc ggagcggctc
      901 gtcgaagacc tcctcgtcga agttcgccga cgcgtaccag acgacgacct tgtcccccgt
      961 gcggatctgc tgcccgcgaa gctccacgtc cgccgtcgcc gtgcgacgga agtgaaggac
     1021 gggcgtcgac cagcggagaa tctcgtccgc gccggtctcc caggcgaggg ccgggtcgtc
     1081 ccgcatccgg ccccactcgt cccggtgctc catgagcgcg agcatcccgt gcgcgatcgc
     1141 ctggcgcgtc gtctcgttcc ccgccacgac gaggaggagg aacatggtgt cgaactcccg
     1201 ctcgctgagc cgttcgccgt cgatctcggc gttgacgagc ttgctgacga ggtcgtcccg
     1261 cgggcgggcg cgccgctcgg cggcgatccg gtggccgtac tcgaagagct cgagcgccgc
     1321 ggggctccgg aagggcagga gccggtagcg gtcgctcgcc gggctgtcgt ggaggacgtc
     1381 ggcgaagtcg gggtccgtgt tcgcgatcat ccggtcgccc cagtcgatca ggcgggcgtt
     1441 gtcctcgtcc ggaacgccga gcaggcgggc gagaacgcgg atcggaagct ccttggcgat
     1501 ctcctcgacg aagtcgaact cgcggcgcag gagcgcgcgg tcgaggatct ccctcgtgag
     1561 ctcgcggagc gcgttctcgt acgcccgcac ggcgcggggg gtgaacggcg ggccgacgag
     1621 gccgcggagg cgcgtgtgcc gaggcgggtc ggtctcgagc atcgactggc gcgcgtcgcg
     1681 cgcatcctcg tccagatcct ccagattcgc gccctgcgcg gaggagtagg tcttggtgtc
     1741 ccgcgagacg gcgacgacgt cggcgtgctt cgtcacgcac cagaagccgc gtccgaacgg
     1801 ccactcgtac caccagaccg gcgc
//
LOCUS       DASZOB010000410         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_149833, whole genome shotgun sequence.
ACCESSION   DASZOB010000410 DASZOB010000000
VERSION     DASZOB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149833"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VG709_06490"
     CDS             <1..882
                     /locus_tag="VG709_06490"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWC32760.1"
                     /translation="RRYDWRPMASPEQTQANVCYRHPKVETAVACSDCGRPICTECMV
                     FGAVGIKCPECAGQPTGMKAATTRARGAAREGAGGIATRALIALTVAGFLLQISDGGI
                     RGVDSSAFREGALFGPLVAEGEWWRLVTAAYLHAGPVHLLLNMLMLWWFGQPLEALLG
                     RARFLGIYAVSILAGSAGALLINPEVPTVGASGAVFGILGAGLILERGGTMVFGGAAL
                     MVVAFNVVLSFAIPNISIGGHFGGLAGGALAILALTRFGRAHAAYGRLGLVGVAGLLG
                     IGVLSIAIAYARVRGYA"
     gene            complement(920..>2396)
                     /locus_tag="VG709_06495"
     CDS             complement(920..>2396)
                     /locus_tag="VG709_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWC32761.1"
                     /translation="GTIVLDADTADERGRAGEDVILVRRETTPDDIHGLMQARGVLTA
                     HGGMTSHAAVVARGMGKPCVAGCEALSIDLHARTVRIGEHERREGDVITIDGGTGAVI
                     LAEVDLVPPQINEDFQTILAWADDVPLAEPEWTEEFRTILDWSDELRRLGVRTNADTP
                     DDARTAREYGAEGIGLCRTEHMFMAEDRLPVVRGMIMARNEEQRRAALAQLLPLQQGD
                     FEGIFEAMAGLPVTIRLLDPPLHEFLPPLEEAESTEMARRIRALHEANPMLGTRGCRL
                     GLMYPEIYAMQVQAIIRAALAVEERTGDAPLVEIKHPLVGFAEELRRLRELTLDTASA
                     ELEQAEREIDYLVGTMIELPRACVRADEIAGAADFFSFGTNDLTQTALGFSRDDAEAG
                     FLTHYLEGDVLADTPCETLDREGVGDLMALAIERARTVKREIKLGICGEHGGDPKSVA
                     FCHELGLDYVSCSPYRVPLARLSAAQAVLAEAGVKTVVVGG"
BASE COUNT          327 a          861 c          837 g          371 t
ORIGIN      
        1 cgccgatacg attggcgccc gatggcctcc ccggagcaga cgcaggcgaa cgtctgctac
       61 aggcacccaa aggtcgagac cgcggtcgca tgctcggact gcgggcggcc gatctgcacc
      121 gagtgcatgg tcttcggcgc ggtcggcatc aagtgcccgg agtgcgcggg ccagccgacg
      181 ggaatgaaag ccgcgacgac ccgcgcacgc ggggctgcgc gcgagggagc gggcggaatc
      241 gcgacgcgcg cgctgatcgc gctcaccgtc gccggattcc tgctccagat ttccgacggc
      301 ggaatccgcg gcgtcgacag ctctgccttt cgcgagggcg cgctcttcgg cccactcgtc
      361 gcggagggcg aatggtggcg cctcgtcacc gccgcttacc tccacgcggg gccggttcac
      421 ctcctcttaa acatgctcat gctctggtgg ttcgggcagc cgctcgaagc gctgctcgga
      481 cgcgcccgct tcctcgggat ctacgccgtc tcgatcctcg ccggatcagc cggcgcgctc
      541 ctgatcaacc cggaagtgcc gacggtcggc gcgtccggcg ccgtcttcgg gatcctcggc
      601 gccgggctca tcctcgagcg gggcggcacg atggtcttcg gaggtgcggc gctgatggtc
      661 gtcgccttca acgtcgtcct gagcttcgcc atcccgaaca tctcgatcgg aggccatttc
      721 ggcggcctcg cgggcggcgc gctcgcgatt ctcgccctga cgcgcttcgg ccgggcgcac
      781 gcggcgtacg gacgcctcgg cctggtcggc gtcgccgggc tcctcgggat cggcgtgctc
      841 agcatcgcga tcgcatacgc ccgcgtccgc ggctacgcgt aaccatcgcg cggcgaccgc
      901 gtcaccgcgc ggcgggacgt cacccgccga cgacgaccgt cttgacgccc gcctcggcga
      961 ggacggcctg ggcggccgag agccgcgcga gcgggacgcg atacggcgag cagctcacgt
     1021 agtcgagccc gagctcgtgg cagaacgcga cggacttcgg gtcgccgccg tgctccccgc
     1081 agatcccgag cttgatctcc cgcttcacgg ttcgcgcgcg ctcgatcgcg agcgccatca
     1141 agtcgccgac gccctcgcgg tcgagcgtct cgcacggggt gtcggcgagg acgtccccct
     1201 cgaggtagtg cgtcaggaag ccggcctcgg cgtcgtcgcg cgaaaagccg agcgccgtct
     1261 gcgtcagatc gttcgtcccg aaggagaaga agtcggccgc gccggcgatc tcgtcggcgc
     1321 gaacacaggc gcggggaagc tcaatcatcg tcccgaccag gtagtcgatc tcgcgctcgg
     1381 cctgctcgag ctcggcggaa gccgtgtcca gggtcagctc gcgcaggcgc cgaagctcct
     1441 ccgcgaaccc gacgagcggg tgcttgatct cgacgagcgg cgcgtcaccg gtgcgctcct
     1501 ccaccgcgag cgccgcgcgg atgatcgcct gcacctgcat cgcgtagatc tccgggtaca
     1561 tcaggccgag acggcaccca cgcgtgccga gcatcgggtt cgcctcgtga agtgcgcgga
     1621 tgcgtctcgc catctcggtc gactcggcct cctcgagcgg cgggaggaac tcgtggagag
     1681 gcgggtcgag cagccggatc gtgaccggaa ggccggccat cgcctcgaag atcccctcga
     1741 agtcgccttg ctggagcggg aggagctgcg caagcgccgc gcggcgctgc tcttcgttcc
     1801 gcgccatgat catgccgcgg acgacgggca gccggtcctc ggccatgaac atgtgctcgg
     1861 tgcggcagag gccgatcccc tcggccccgt attcgcgtgc cgtgcgagcg tcgtccggcg
     1921 tgtcggcgtt cgtgcgcacg cccaggcgcc gcagctcgtc cgaccagtcg aggatcgtcc
     1981 ggaactcctc ggtccactcg ggctccgcta gcggcacgtc gtccgcccag gcaaggatgg
     2041 tctggaaatc ctcgttgatc tgcggcggga cgaggtcgac ctcggcgagg atcacggcgc
     2101 cggtcccgcc gtcgatcgtg atgacgtcgc cctcgcgccg ctcgtgctcg ccgatcctga
     2161 ccgtgcgcgc atgaagatcg atcgagagcg cctcgcagcc cgcgacgcag ggcttcccca
     2221 tcccgcgcgc gaccacagcg gcgtgcgacg tcatgccgcc gtgcgccgtg aggacgccgc
     2281 gggcctgcat gaggccgtgg atgtcgtctg gagtcgtctc ccggcgaacg aggatgacgt
     2341 cttcgccggc gcgccctcgc tcgtcagccg tgtcggcgtc gagcacgatc gtgccg
//
LOCUS       DASZOB010000411         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_119042, whole genome shotgun sequence.
ACCESSION   DASZOB010000411 DASZOB010000000
VERSION     DASZOB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119042"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VG709_06500"
     CDS             complement(<1..495)
                     /locus_tag="VG709_06500"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWC32762.1"
                     /translation="MRRLVVSLVVIVVVLLVADFGLRFWAESWFASQVQQGAELREEP
                     DLDLRGFPFMLVMARGRLPSASVEAEAFSSGGLKVQRLVLELHDVRFSPGRLVSRGSG
                     TIRSDGGTGRATVTQEDLTAYANLRGYPGRIAFHDGRATVTTMQQPLGVEVEVRATGD
                     LSINE"
     gene            complement(540..>1889)
                     /locus_tag="VG709_06505"
     CDS             complement(540..>1889)
                     /locus_tag="VG709_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC32763.1"
                     /translation="RDGVRRIRAGLGEARAGLDRLAAEVVEPARRNLEEAWGDLKRAT
                     VAKADPVYRDVVQSVGTALALVSGRCPDASGMGLPLLVVEEQACEPGSRVAPGYDGLA
                     AALREVSSGLGRARDGLARIDDGLAELAGGLRSAGPGIDRLADGVERMIGGLDRIIPG
                     LSQLYRGLVVGFAQAERAGLIPAGSGDVALTASLVRAFPKLREQLSFFVGERGRSTRL
                     FVTLDSPPYEASALDTSMEVRDLAELSLAKTPLDAASVSTTGGSAFFADVRDIQSRDF
                     RKIVWVVVLGIFLVLALLLRSVVAPIYLVLTVMLSFLSTLGIATMVFQGLLGHDGITW
                     WIPPFLFVMLVALGADYNIFLTSRIREEAERTDTREAVARGLTLTGHVITSAGLILAG
                     TFAALLFTPMQGLIQMGFATSIGILIDTFIVRSLVVPAFAVLLGRHNWWPSRRARTA"
BASE COUNT          305 a          694 c          660 g          230 t
ORIGIN      
        1 ctcgttgatc gagaggtcgc ccgtcgcgcg cacctcgacc tcgacgccga ggggctgctg
       61 catcgtcgtg accgtcgcgc ggccgtcgtg gaacgcgatc cggccggggt agccgcgcag
      121 gttcgcgtac gcggtgaggt cctcctgcgt caccgtcgcc cgccccgtcc caccgtcgga
      181 gcggatcgtc ccggaccccc tcgagacgag ccgtccgggg ctgaatcgaa cgtcgtgcag
      241 ctcgagcacc aaccgctgga ccttcagccc gccggagctg aacgcctccg cctcgacgct
      301 cgcggacggc aggcgccctc gcgccatgac gagcatgaac gggaacccac ggaggtccag
      361 gtccggttcc tcacgcagct ccgcgccctg ctgcacctgc gacgcgaacc agctctccgc
      421 ccagaaccgc aggccgaagt cggccacgag caggacgacg acgatgacca cgagcgagac
      481 gacgagtcgg cgcacgcgct cttgtctacc ctgcggaacg cggaccgacc cgctcggcgt
      541 caggccgtgc gcgcgcggcg gctgggccac cagttgtggc ggccgaggag gaccgcgaac
      601 gccggcacca cgaggctccg gacgatgaac gtgtcgatga ggattccgat gctcgtcgcg
      661 aaccccatct ggatcaggcc ctgcatcggc gtgaacagca gcgccgcgaa cgtcccggcg
      721 aggatcagcc cggctgacgt gatcacgtgt cccgtcagcg tgaggccccg cgcgacggcc
      781 tcgcgcgtgt cggtgcgctc ggcctcctcg cggatccgcg acgtgaggaa gatgttgtag
      841 tcggcaccga gcgcgaccag catcacgaag aggaacggag ggatccacca cgtgatcccg
      901 tcgtgcccga gcaggccctg gaacaccatc gtcgcgatcc cgagcgtgga caggaacgac
      961 agcatcaccg tcagcacgag gtagatcggg gcgacgacgc tgcggagcag cagcgcgagc
     1021 acgaggaaga tcccgaggac caccacccag acgatcttgc ggaagtcacg gctctggata
     1081 tcacggacgt cggcgaagaa cgcgctcccg ccggtcgtgc tgacggaggc cgcgtcgagc
     1141 ggcgtcttcg cgagcgagag ctcggcgaga tcacggacct ccatggaggt atccagcgcg
     1201 ctcgcctcgt aggggggact gtcgagggtg acgaagaggc gcgtcgagcg tccgcgctca
     1261 ccgacgaaga aggacagctg ctcgcggagc ttcgggaacg cgcggacgag cgacgcggtc
     1321 aacgcgacgt cgccgctgcc ggcggggatc agcccggccc gctcggcctg cgcgaacccg
     1381 acgacgagcc cccggtagag ctgggagaga ccggggatga tccggtcgag tccgccgatc
     1441 atgcgctcga cgccgtccgc gagccggtcg atgcccggcc ctgcggatcg cagcccgccg
     1501 gcgagctcgg ccagcccgtc gtcgatgcga gcgaggccgt cccgcgcccg gccgagcccg
     1561 gacgagacct ctcgcagcgc ggccgccaga ccgtcgtacc ccggggcgac cctggagccg
     1621 ggctcacacg cctgctcctc gaccacgagg agcggcaatc ccatgccgct cgcgtcgggg
     1681 cagcggcccg agaccagggc gagcgcggtg ccgacgctct gcacgacgtc gcggtagacg
     1741 gggtcggcct tggcgaccgt ggctcgcttc aggtcccccc acgcctcctc gaggttcctg
     1801 cgcgccggct ccacgacctc cgccgccagc cggtcgagcc cggcccgcgc ttcgccgaga
     1861 cccgcgcgga tccgccggac gccgtcgcg
//
LOCUS       DASZOB010000412         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_44616, whole genome shotgun sequence.
ACCESSION   DASZOB010000412 DASZOB010000000
VERSION     DASZOB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44616"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VG709_06510"
     CDS             complement(<1..730)
                     /locus_tag="VG709_06510"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HWC32764.1"
                     /translation="MIDLRSDTVTRPTPAMRRAMAEAEVGDDVYGDDPTVNGLQERVA
                     EITGKDAALYTVTGMMANQIALRFFVRTGHEAVCESTAHVTTVEKHASAAVSGITFRP
                     VVAARGLLTPELVREALAPDPLGLRVVDLVTVENTHQIGGGTVLPLESLRGIRDVTSE
                     AGVPLYMDGARIWNASVATGVPVRDYAEHVDALMFCFSKGLGAPIGSILCGPGDLIEE
                     ARRVRVHLAGAWRQAGDIAAAALVA"
     gene            complement(727..891)
                     /locus_tag="VG709_06515"
     CDS             complement(727..891)
                     /locus_tag="VG709_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32765.1"
                     /translation="MATVRCPSCGDDIVPLPVEREEGATQYVCPACRRRLTIDVVDLY
                     LALEEDEAAG"
     gene            complement(902..1933)
                     /locus_tag="VG709_06520"
     CDS             complement(902..1933)
                     /locus_tag="VG709_06520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HWC32766.1"
                     /translation="MARFGVLTGGGDCPGLNAVIRAVTRRGHEDHGHRIVGFRNGYRG
                     VLEGSVMELTPRSARGILHRGGTILGTSRADPFRLEGGLDLLRENLETYRIDGLIVIG
                     GEGTLGSAAKLSAEGINVVGVPKTIDNDIAATDFTFGFDTAVQVATDAIDRLHTTAES
                     HNRVMVLEVMGRHTGWIGLYAGLAGGADVILIPEHPFDINDVCSHLLHRSHEGHTFSI
                     VVVSEGATPREGTLERPSYETDEFGRPKLGGISSVIAAEIERRTGFETRVTILGHVQR
                     GGSPTAADRVLATRFGVAAIDLASDGRWGRMVALRGGDIVDCQLDEAVGELKRVPDEL
                     YGVAEVFFG"
     gene            2014..>2619
                     /locus_tag="VG709_06525"
     CDS             2014..>2619
                     /locus_tag="VG709_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWC32767.1"
                     /translation="MAILAIDAGTTGVKVLVVAEDGSVTGSGHREFPQHFPRPGWVEH
                     DPDDWWTATVEAATDALGVARIDAASLVAVGITNQRETTVLWERETLRHLHRAIVWQD
                     RRTAPLCEELRRQGWEPKIRQRTGLVIDPYFSGTKLAWLLDHVEGASDAARDGRLAFG
                     TVDAYLVARLTGGRSHTTDHTNASRTMLFDIGKLRWDDELLE"
BASE COUNT          379 a          946 c          909 g          385 t
ORIGIN      
        1 gggccaccag ggccgcggcg gcgatgtccc ccgcctgccg ccaggcgccg gcgaggtgga
       61 cccgcacccg ccgggcctcc tcgatcaggt cgccggggcc gcacaggatg gacccgatcg
      121 gagcccccag gcccttcgag aagcagaaca tcagcgcgtc gacgtgctcc gcgtagtcgc
      181 gaaccggcac gcccgtcgcc acggacgcgt tccagatccg ggcgccgtcc atgtacagcg
      241 gcaccccagc ctcgctcgtg acgtcgcgga tgccgcggag ggactcgagc gggagcacgg
      301 tgccgccgcc gatctggtgg gtgttctcga ccgtgacgag gtcgacgaca cggagcccga
      361 gcggatccgg tgcgagcgcc tcgcggacga gctcgggcgt gaggagccct cgcgctgcga
      421 cgaccggacg gaacgtgatc cccgagaccg cggcggacgc gtgcttctcc acggtcgtga
      481 cgtgcgccgt ggactcgcat acggcctcgt ggcccgtacg gacgaagaaa cgcagcgcga
      541 tctggttggc catcatgccg gtcacggtgt agagcgccgc gtccttgccg gtgatctcgg
      601 cgacgcgctc ctgcaagcca ttcacggtcg ggtcgtcgcc gtagacgtcg tccccgacct
      661 cggcctcggc catcgcgcgg cgcatcgcgg gagtcgggcg cgtgacggtg tccgagcgga
      721 gatcgatcat ccggccgcct cgtcctcctc gagcgcgagg tagaggtcaa cgacgtcgat
      781 cgtgaggcgt cgccggcatg ccgggcacac gtactgggtc gctccctctt cgcgctcgac
      841 agggagcggc acgatgtcgt cgccgcacga gggacaccga acggtcgcca cggccgagac
      901 cctacccgaa gaacacctcg gcgacgccgt agagctcgtc cgggacgcgc ttcagctctc
      961 cgaccgcctc gtcgagctgg cagtcgacga tgtcgcctcc gcggagcgcg accatccggc
     1021 cccatcgccc gtccgacgcc agatcgatgg ccgcgacccc gaacctcgtg gcgagcacgc
     1081 ggtcggccgc cgtcgggctt cctccgcgct ggacgtgtcc caggatcgtg acccgcgtct
     1141 cgaagcctgt ccgacgctcg atctcggccg cgatcacgct cgagatgccg cccagcttgg
     1201 ggcgcccgaa ctcgtcggtc tcgtagctcg gccgctcgag cgtcccctcc ctcggggtcg
     1261 ccccttccga tacgacgacg atcgagaacg tgtgcccctc gtggctacgg tgcagcaggt
     1321 ggctgcagac gtcgttgatg tcgaacgggt gctcggggat gaggatcacg tcggcgccgc
     1381 cggcgagccc cgcgtagagc ccgatccagc ccgtgtgccg ccccatcacc tcgagcacca
     1441 tgacgcggtt gtggctctcg gccgtggtgt ggagccggtc gatcgcgtcg gtggcgacct
     1501 ggaccgccgt gtcgaacccg aacgtgaaat cggttgcggc gatgtcgttg tcgatcgtct
     1561 tcgggacgcc gacgacgttg atcccctcgg ccgacagctt cgctgcgctg ccgagggttc
     1621 cctcgccgcc gatcacgatg aggccgtcga tccggtaggt ctcgaggttc tcgcggagca
     1681 ggtcgaggcc gccctcgagc cggaacgggt ccgcgcgaga cgttccaagg atcgtgccgc
     1741 cacggtggag gatcccccgc gcgctccggg gcgttagctc catgacgctt ccttcgagga
     1801 ccccgcggta gccgttacgg aagccgacga tccggtgccc gtggtcctcg tgccccctgc
     1861 gcgtgacggc ccggatgacg gcgttgagcc cgggacagtc gccgccgccg gtcagcacgc
     1921 cgaacctcgc cacgcgatct ccctcgtcgg gctcgcgcca ttctagggag cggcatggcg
     1981 gcgcatccgc cgtgccgggt agcctcaggc gccgtggcga tcctcgcgat cgatgccggt
     2041 acgaccggcg tgaaggtgct cgtcgtcgcg gaggacggct ccgtcaccgg gagcggtcac
     2101 cgcgagttcc cgcagcactt cccccgccca gggtgggtgg agcacgatcc cgatgactgg
     2161 tggacggcga cggtggaggc cgccaccgac gcgctgggcg tcgcgcggat cgatgccgcg
     2221 tcgctcgtcg cggtcggcat cacgaaccag cgcgagacca ccgtgctgtg ggagcgcgag
     2281 accctccgcc acctccatcg cgcgatcgta tggcaggacc gccggaccgc accgctctgc
     2341 gaagagctcc gtcggcaggg atgggagccg aagatccggc agcggaccgg cctcgtcatc
     2401 gacccctact tctccggcac gaagctcgcc tggctcctcg accacgtcga gggcgcaagc
     2461 gacgcggctc gcgacgggcg actcgcgttc ggcacagtcg acgcgtacct cgtcgcacgg
     2521 ctcaccggcg ggcggagcca cacgaccgac cacacgaacg cgtcgcgaac gatgctcttc
     2581 gatatcggga agctgcggtg ggacgacgag ctcctcgag
//
LOCUS       DASZOB010000413         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_129728, whole genome shotgun sequence.
ACCESSION   DASZOB010000413 DASZOB010000000
VERSION     DASZOB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129728"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VG709_06530"
     CDS             <1..599
                     /locus_tag="VG709_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC32768.1"
                     /translation="VLTLVDTGTWMSERRIGAAFERMGRRPTAVRQIVISHSHGDHAG
                     GAASLRETCQAPVIVGAADAPVIEGREPYADAPATWARASYSWLARFPRFRPDRTVAE
                     EEELEGGLLVILTPGHTPGHLAFHAPDNPLLFAGDSVWNLGTVRLDWRSFSPEWERNR
                     ESVRRLAALDVDGVFVGHGPPIRRDARRRLRALVRDEV"
     gene            687..>1547
                     /locus_tag="VG709_06535"
     CDS             687..>1547
                     /locus_tag="VG709_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWC32769.1"
                     /translation="MDTGDTAWLLTSAALVMFMTPGLALFYGGLVRAKNVVGTIMQSF
                     FALALVTVLWVLVGYTLAFGSDHGGLIGGLEFWGLRGVGQAPSDVYATTTPHIAFSTY
                     QLMFAIITPALITGAVAERMKFAGYVLFLALWSLVVYAPLAHWVWGGGWLATLGALDF
                     AGGTVVHINAGAAALAAILYLGKRRGFGRDAFVPHNVPMVITGAAILWFGWFGFNAGS
                     ALGANGLAATAFATTHIATAAAIIGWLIPETMRHGRPTTIGAATGAVAGLVAITPAAG
                     FVEPWAAIVIG"
BASE COUNT          198 a          554 c          554 g          241 t
ORIGIN      
        1 gcgtcctgac gctagtggac accggcacgt ggatgagcga gcggcggatc ggcgcggcct
       61 tcgagcggat gggacgtcgc ccgaccgccg tccgccagat cgtgatctcc cacagccacg
      121 gcgaccacgc cggcggcgcg gcgagcttgc gagagacctg ccaggcgccc gtgatcgtgg
      181 gagctgcgga tgctccagtc atcgaggggc gcgagccgta cgcggatgcg cccgcgacgt
      241 gggcgcgggc gtcgtacagc tggctcgccc ggttcccgcg gttccgtccc gaccgcacgg
      301 tcgccgagga ggaggagctc gagggcgggc tcctcgtcat cctgacgccg ggccacacgc
      361 cggggcatct cgcgttccac gcccccgaca acccgctgct gttcgcgggc gatagcgtgt
      421 ggaacctcgg gaccgtccgc ctcgactggc ggtcgttctc gccggagtgg gagcggaacc
      481 gcgagagcgt ccgtcggctc gccgctctcg acgtcgacgg ggtcttcgtg gggcacggcc
      541 cacccatccg ccgcgacgcg cgacgccgcc tccgcgctct cgtccgcgac gaggtttaac
      601 ggcgccgtaa cagccgggaa accgccgcga aacgtgcgcg gcttacaaac gccctcgtcc
      661 acgaccgagg gggaaccatg tccgggatcg acaccggaga caccgcctgg ctcctgacgt
      721 ctgcggcgct cgtgatgttc atgacgcccg ggctcgcgct gttctacgga gggctggtcc
      781 gcgccaagaa cgtcgtcggc acgatcatgc agtcgttctt cgcgctggcg ctcgtgaccg
      841 tcctgtgggt cctcgtcggg tacacgcttg cgttcggctc cgaccacggc ggactcatcg
      901 gcgggctcga gttctggggc ctccgcggcg tcgggcaggc gccgagcgac gtgtacgcga
      961 cgaccacgcc gcacatcgcg ttcagcacgt accagctgat gttcgcgatc atcacgccgg
     1021 cgctcatcac cggcgccgtc gcggagcgca tgaagttcgc cggctacgtg ctcttcctcg
     1081 cgctctggtc gctcgtcgtc tacgcgccgc tcgcccactg ggtgtggggc ggcggctggc
     1141 tcgcgacgct cggcgcgctc gacttcgcgg gcgggaccgt cgtgcacatc aacgccggag
     1201 ccgcggccct ggcggcgatc ctgtacctcg ggaagcggcg cggattcggc cgcgacgcgt
     1261 tcgtgcccca caacgtcccg atggtcatca ccggcgccgc gatcctgtgg ttcggctggt
     1321 tcgggttcaa cgccggctcc gcgctcggcg cgaacgggct tgccgcgacg gcgttcgcga
     1381 ccacgcatat cgcgaccgcg gcggcgatca tcgggtggct gatccccgag acgatgcgtc
     1441 acggccgccc aactaccatc ggggcggcga cgggcgccgt cgccgggctc gtcgcgatca
     1501 cgccggcggc ggggttcgtt gagccgtggg cggcgatcgt gatcggt
//
LOCUS       DASZOB010000414         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_99855, whole genome shotgun sequence.
ACCESSION   DASZOB010000414 DASZOB010000000
VERSION     DASZOB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99855"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VG709_06540"
     CDS             <1..211
                     /locus_tag="VG709_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32770.1"
                     /translation="AWAILGQALGWQVALGGAAVLAGGALVVVYEGTEARSVEAPVLV
                     PEERPASTALVSNEASPSGRSVILK"
     gene            228..1229
                     /gene="glpX"
                     /locus_tag="VG709_06545"
     CDS             228..1229
                     /gene="glpX"
                     /locus_tag="VG709_06545"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWC32771.1"
                     /translation="MIAGGDGAVPDRNLALELARVTEAAALGAARWVGRGDKNAADQA
                     AVDGMRAMLDTGAMSGVVVIGEGEKDEAPMLYNGEEVGNGQPPDVDVAVDPLEGTRLT
                     SVGQPNAIAVIAVAERGTMFFPGAAVYMDKIAGGSEIADAISIDATPEENVRRAAEAK
                     DVKPSDISVVVLDRPRNENLIAALRECGAKVVLITDGDVAPAIAAANPSRPVDLMMGI
                     GGTPEGVIAAAAVKCLGGTVQGRLWPRDDAERQALADGGFDVDRVLTTDELVAGDNVF
                     VAATGVTDGALLDGVRYTAEGAVTDSIVMRSRSGTVRRVQAWHAFEKLSRLSGRQYR"
     gene            1303..>1654
                     /locus_tag="VG709_06550"
     CDS             1303..>1654
                     /locus_tag="VG709_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWC32772.1"
                     /translation="MEPRAQAALVGAIVAWASAFAAVKVLLDAGFAGEDGALARYGVA
                     LPGFAYLLLRAGGLPGLGLADAARIAAAGVLIVATYHISLNLGTAHTTAGTAALVVAL
                     APALTVLLAAALGYE"
BASE COUNT          233 a          567 c          614 g          240 t
ORIGIN      
        1 cgcgtgggcc atcctcgggc aggcgttggg ttggcaggtg gcgctcggcg gagccgcagt
       61 cctcgccggc ggcgcgctcg tcgtcgtcta cgaaggcacg gaggcgcgct cggtcgaggc
      121 gcccgtcctc gtgcccgagg aacgacccgc gtctacggca ctcgtttcaa atgaagcttc
      181 gccgtctggc cgaagcgtca tcttgaaatg agttttagga tgggcgagtg atcgcaggcg
      241 gagacggggc cgtccccgac cgcaacctcg cgctcgagct cgctcgtgtg accgaggccg
      301 ccgcgctcgg cgccgctcgc tgggtcgggc ggggagacaa gaacgccgcc gaccaggccg
      361 cggtcgacgg gatgcgcgcg atgctcgaca cgggcgccat gagcggcgtc gtcgtgatcg
      421 gagagggcga gaaggacgag gcgccgatgc tctacaacgg ggaggaggtc ggtaacggcc
      481 agccgcccga cgtcgacgtc gccgtcgacc ccctggaggg cacgcggctc acctcggtcg
      541 ggcagcccaa cgcgatcgcg gtgatcgcgg tggcggagcg cggcacgatg ttcttccccg
      601 gggccgcggt gtacatggac aagatcgcgg gcggcagcga gatcgcggat gcgatctcca
      661 tcgacgcaac gcccgaggag aacgtgcgcc gcgcggcgga ggcgaaggac gtgaagccga
      721 gcgacatctc ggtcgtcgtc ctcgaccgtc cgcgcaacga gaatctgatc gccgcgctgc
      781 gagagtgcgg cgccaaggtc gtcctgatca ccgacggaga cgtcgcgccc gcgatcgccg
      841 ccgcgaaccc gtcgcgaccc gtcgacctca tgatgggaat cgggggaacc cccgagggcg
      901 tcatcgcggc ggcggccgtc aagtgcctcg gcgggacggt gcagggccgg ctctggccgc
      961 gcgacgacgc cgagcggcag gcgcttgcgg acggcggatt cgacgtcgac cgcgtgctca
     1021 ccacggatga gctcgtcgct ggcgacaacg tgttcgtcgc cgcgacgggc gtgaccgacg
     1081 gcgcgctcct cgacggcgtg cgttacacgg ccgagggggc ggtcacggac tcgatcgtca
     1141 tgcgctcccg ctccgggacg gtaaggcgcg tccaggcgtg gcatgccttc gagaagctgt
     1201 cgcgcctctc cggccgccag tatcgctgag gtagagccag ccgtaccgtg agtctcgtga
     1261 cagccggtcc acgccggttc ccgggtggac gttcgaaccg gtgtggaacc acgtgcccag
     1321 gcggctctgg tcggcgcgat cgtcgcgtgg gcctcggcgt tcgccgccgt caaggtgctc
     1381 ctcgacgccg gattcgcggg cgaggacggc gcgctcgcac gctacggagt cgccctcccg
     1441 ggcttcgcgt acctcctgct tcgagcgggg ggtctgccgg ggctcggtct tgcggacgcg
     1501 gctcgcatcg cggcggccgg cgtgctcatc gtcgcgacct accacatctc gctgaacctc
     1561 ggaaccgcgc atacgaccgc cggaacggcg gcgctcgtcg tcgccctcgc gccggcgctc
     1621 acggttctgc tcgccgccgc gctcggctac gaat
//
LOCUS       DASZOB010000415         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_99856, whole genome shotgun sequence.
ACCESSION   DASZOB010000415 DASZOB010000000
VERSION     DASZOB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99856"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1699
                     /gene="carB"
                     /locus_tag="VG709_06555"
     CDS             <1..>1699
                     /gene="carB"
                     /locus_tag="VG709_06555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWC32773.1"
                     /translation="VRPVFKTVDTCAGEFRATTPYYYSTYERSSEVRQAERPRVVILG
                     SGPNRIGQGIEFDYACVHAAFALREAGFETVMVNCNPETVSTDYDTSDRLYFEPLTVE
                     DVVAVCEAEEPVGVVVQLGGQTPLGIAAELETEGFPVLGTSPASIDLAEDRPKFAALL
                     REIGIAHPPHGQASSREEAVAVAQRIGFPVVVRPSYVLGGRAMEIVYTEEELAGYLAS
                     AAAADPHHPVLVDRFLEGALEVDVDAVSDGEETFVAAVMEHIEEAGVHSGDSSAVVPP
                     VSLSDEELDEIERITRQIAAALEVRGLLNIQLAIKDERVWVLEANPRASRTVPFVSKA
                     TGVPLAKVAARVLVGSRLAELRAEGLLPPRPSHYRRLRHVAVKAVVLPFGRFPGQDTV
                     LGPEMRSTGEVMGIATDLGTALGRAQSATGTSLPQKGTVFVSVSNRDKRAIVFPVKRL
                     ADLGCRVGATRGTAGLLARAGIEVSCVAKVSEGHPNAVDLIKAGEVDLVINTPFGRRP
                     RSDGSYIRMAAAARGGPCVTTLHGTFAVVQGVEALRRGGEEARCLQEYHAGAWEGAHL
                     "
BASE COUNT          233 a          575 c          633 g          258 t
ORIGIN      
        1 cgttcgcccc gtgttcaaga ccgtcgacac ctgtgccggc gagttccgcg cgacgactcc
       61 ttactactac tcgacgtacg agcgctcctc ggaggtgcgt caggcggaga ggccgcgcgt
      121 cgtgatcctc gggtcgggac ccaaccggat cggacagggg atcgagttcg actacgcgtg
      181 cgtgcacgcg gcgttcgcgc tccgcgaagc cgggttcgag accgtcatgg tgaactgcaa
      241 ccccgagacg gtctcgacgg actacgacac gagcgatcgc ctgtacttcg agccgctcac
      301 ggtcgaggac gtcgtcgcgg tgtgcgaggc cgaggagccg gtcggcgtcg tcgttcagct
      361 cggcggccag acgccgctgg gcatcgcggc ggagctcgag accgaggggt tcccggtgct
      421 ggggacctcg ccggcgtcga tcgacctcgc tgaagaccgg ccgaagttcg cggcgctgct
      481 ccgcgagatc ggcatcgcgc atccgccgca cgggcaggcg tcgtccaggg aagaggcggt
      541 cgccgtcgcg cagcgcatcg ggttcccggt tgtcgtgcgt ccgtcgtacg tcctcggcgg
      601 ccgcgccatg gagatcgtgt acaccgagga agagctcgcg gggtacctgg cgagcgctgc
      661 ggccgccgat cctcaccacc cggtcctcgt cgaccgcttc ctcgaagggg cgctcgaggt
      721 cgacgtcgac gcggtctccg acggcgagga gacgttcgtc gcggccgtga tggagcacat
      781 cgaagaggcg ggggtccact ccggcgactc atctgcggtg gttccgccgg tctcgctgtc
      841 ggacgaggag ctcgatgaga tcgagcgcat caccaggcag atcgcggcag cgctcgaggt
      901 gcgcgggctg ctgaacatcc agctcgcgat caaggacgag cgggtatggg tcctggaggc
      961 gaacccgcgg gcgtcgcgca ccgttccatt cgtgtcgaag gccaccggcg tcccgctcgc
     1021 gaaggtcgcg gcgcgcgtgc tcgtcgggtc gcggctcgcg gagcttcgtg ccgagggcct
     1081 gctccctccc cgcccctctc actaccggcg cctgcgccac gtcgccgtga aggccgtcgt
     1141 gctccccttc gggcgcttcc cgggacagga cacggtgctt gggcccgaga tgcgctcgac
     1201 cggcgaggtg atggggatcg cgaccgacct cgggacggcg ctcggccgcg cgcagtcggc
     1261 gaccggcacg tcgctcccgc agaaggggac cgtgttcgtg agcgtctcga accgcgacaa
     1321 gcgtgcgatc gtcttcccgg tgaagcggct cgcggatctc gggtgccgcg tcggcgccac
     1381 gcgcggcacc gccgggctcc tcgcgcgcgc cggcatcgag gtcagctgcg tcgcgaaggt
     1441 gtcggagggg catccgaacg ccgtcgacct catcaaggcg ggtgaggtcg atctcgtgat
     1501 caacacgcct ttcggcaggc gcccgcggag cgacggctcg tacatccgca tggcggcggc
     1561 cgcacgcggg ggcccgtgcg tcacgacgct gcacggcacg ttcgcggtcg tgcagggcgt
     1621 tgaggcgctg cgccgtggcg gcgaggaggc gcggtgcctc caggagtacc atgccggggc
     1681 gtgggaggga gcgcacctg
//
LOCUS       DASZOB010000416         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29408, whole genome shotgun sequence.
ACCESSION   DASZOB010000416 DASZOB010000000
VERSION     DASZOB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29408"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VG709_06560"
     CDS             complement(<1..956)
                     /locus_tag="VG709_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWC32774.1"
                     /translation="MKAVTVVPKRTGTAALSDVPEPPAEEGSVLVEAVAVGVCGTDEE
                     IVGGGYGWPPPGGERLVLGHESLGRVVEADAAKGLAPGDLVVGIVRHPDPVPCANCAI
                     GEWDMCRNGRYTEHGIKERDGFMRERYRLEPARTIKVDPSLGELGVLLEPTTIVAKAW
                     EHLDRIGARARWEPRTCLVTGAGPVGLLAAMLGVERGLDVHVLDRVETGQKPKIVDAL
                     GAHYHSGDIDDSHVTDYDVVVEATGVGELAFTALRHTAPNGVVALTGISSSDRMFPVD
                     SDLIGEDMVLNNIALFGSVNANRRHYEHAAQALAAADPGWLSS"
     gene            1010..1654
                     /locus_tag="VG709_06565"
     CDS             1010..1654
                     /locus_tag="VG709_06565"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWC32775.1"
                     /translation="MGRVIDVSLGIHPEMLTWPGDPGVGVRPAKRLDRGDSSNVSELQ
                     LGTPTGTHVDAPWHFVEGGATVDDLDAEILVGPAVVGDMRHVEDRIGADDLERLDLAP
                     GTSRLLFRTRNSDLWASGRRDFPDDYVALGEDGAEWLVAHEVKLVGTDFLSIERKRED
                     GGHPVHVALLEAGVVIVEGLDLSEVTPELYLLYCLPLKIVGVDGAPARVFLAEP"
BASE COUNT          266 a          625 c          644 g          285 t
ORIGIN      
        1 gacgacaacc atccggggtc ggcggcggcg agcgcctgcg cggcgtgctc gtagtggcgg
       61 cggttcgcgt tcaccgagcc gaacagcgcg atgttgttga ggaccatgtc ctcgccgatg
      121 aggtcggagt cgaccgggaa catccggtcg ctcgacgaga tccccgtaag cgccacgacg
      181 ccgttcgggg cggtgtgccg aagcgccgtg aacgcgagct cgccgacgcc ggtcgcctca
      241 accacaacgt cgtagtcggt gacatgggag tcgtcgatgt cgccggagtg gtagtgcgcg
      301 ccgagcgcgt cgacgatctt aggcttctgc cccgtctcga cccggtcgag cacgtggacg
      361 tcgaggccgc gctcgacgcc gagcatcgcc gcgaggagtc ccacggggcc ggcgcccgtg
      421 acgaggcagg tcctcggctc ccatctcgca cgcgcgccga tgcggtcgag gtgctcccac
      481 gccttggcga cgatcgtcgt cggctcgagg agtacgccga gctcgccgag cgacgggtcg
      541 accttgatcg tgcgcgcggg ctcgaggcgg tagcgctcgc gcatgaaccc gtcgcgctcc
      601 ttgatgccgt gctcggtgta gcggccgttg cggcacatgt cccactcgcc gatcgcgcag
      661 ttcgcgcacg gcaccggatc gggatggcgg acgatgccga cgacgaggtc gccgggcgcg
      721 aggcccttcg cggcgtccgc ctcgacgacc cgccccagcg attcatggcc gagcaccagg
      781 cgctcacctc ccggaggcgg ccacccgtag ccaccgccga cgatctcctc gtccgtcccg
      841 cagacgccga cggcgacggc ctcgacgagg acggatcctt cctcggccgg cggctccggc
      901 acgtccgaga gcgccgcggt tccggttcgc ttcgggacga ccgtgacggc cttcatcgtc
      961 gactaccctg ccgttcgagg gacgaccgcg ccagccggaa ggagcgtccg tgggccgggt
     1021 gatcgacgtc tcgctcggga tccatcccga gatgctgacg tggccgggcg acccgggcgt
     1081 cggcgtccgc ccggcgaagc gccttgatcg cggggactcc tcgaacgttt cagaactgca
     1141 gctcgggacg cccaccggaa cgcacgtcga tgcgccatgg catttcgtcg agggtggggc
     1201 aacggtcgat gacctggatg ctgagatcct cgtcgggccg gccgtcgtcg gcgatatgcg
     1261 acacgtcgag gaccgcatcg gcgccgacga cctggagcgg ctcgatctgg cgcccggcac
     1321 gagccggctg ctgttccgga ctcggaactc ggacctttgg gcgagcggca gacgcgactt
     1381 ccctgacgac tacgtcgcgc tgggtgagga cggagcggag tggctcgtcg cgcacgaggt
     1441 gaagctcgtc gggacggact tcttgtcgat cgagaggaag cgcgaggacg gcgggcatcc
     1501 ggtccacgtc gcgctcctcg aggccggcgt cgtcatcgtc gaggggctgg acctgtcgga
     1561 ggtaacgccc gagctctacc tgctctactg cctgccgctc aagatcgtcg gcgtcgacgg
     1621 ggcgccggca cgcgtcttcc tcgccgagcc ctagctactc gttccgctcg gcgtaccaga
     1681 ccatgtggtc gtagggccat cccgccgaag cgagatgttc gatgagcagc gtctcgtgcc
     1741 gcgtcaccgg cgacccgcga gtatggatcc gtacgacgac gcggtacatc tgagaagcgg
     1801 tcgaacggtc atccgtctcc
//
LOCUS       DASZOB010000417         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_186859, whole genome shotgun sequence.
ACCESSION   DASZOB010000417 DASZOB010000000
VERSION     DASZOB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186859"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VG709_06570"
     CDS             <1..1408
                     /locus_tag="VG709_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32776.1"
                     /translation="VLPDRAMGVEGGLVDVASRMSSIFGVDAADTLRSFPNAAPELVE
                     TEHAAGGRAVFLRARRLDGSAGGETTRALRDALARLVEPDIEIDNEEVFALHRVNGGR
                     DVYFLVNTTFEPQAMTVRMPGELDAAVWDPSSGEERVPESRVADGRTELALELGPVGS
                     VVITPEVRGTRAATPALGRPAATVAVDGEWRFRAEDPNALVLDRWRAAPEAPGASAEG
                     YAAPDVDDDGWQPVGPGAWAFQLPSEPDRPWPIPVWYRFSFATDRVPSSLHLVVDGFA
                     GSDRRVFLNGEEVTTEPVRSPFDSQMKTIDLSGHVGEGRNVLAVRLTLESSTDGVTDL
                     LKLIGGFGVSRGDDGVYRIVPPVERVKPASWTDQGYPFYSGVGVYSTAVEVPPGFAAG
                     RVFLEAPAGDDALEVVVNGRSAGVRLWPPYAAEVTGLLRDGDNEIELRVANTLANLLN
                     GDERASGLAEEPRLVAYG"
     gene            1401..1910
                     /locus_tag="VG709_06575"
     CDS             1401..1910
                     /locus_tag="VG709_06575"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HWC32777.1"
                     /translation="MDERGEAALKVRELSAKAFAPYGRVIEAPSRPGDASGPGWTWWA
                     ETVLLSGDGRPWGVGYLSLEPTDPRFDWAERHMRTLEAIVPMAGDALVYVGPAEHPEE
                     PARLPPLERFEVFRVPAGTGVVMDRGVWHGAPFAADGPGAAMVLILEGTGRSDVTIVR
                     FPPVAFERE"
     gene            1918..2586
                     /locus_tag="VG709_06580"
                     /pseudo
     CDS             1918..2586
                     /locus_tag="VG709_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2589..2816)
                     /locus_tag="VG709_06585"
                     /pseudo
     CDS             complement(2589..2816)
                     /locus_tag="VG709_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019683745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          394 a          918 c         1071 g          433 t
ORIGIN      
        1 ggtccttccc gaccgggcga tgggagtgga aggtgggctc gtcgacgtcg cgtcgcggat
       61 gtcgtcgatt ttcggtgtgg atgccgcgga cacgctgcgg tcgttcccga acgccgcccc
      121 cgagctcgtc gagaccgagc atgcggcggg cgggcgcgcg gtcttcctgc gggcgcggcg
      181 tctcgacgga tccgccgggg gggagacgac ccgagcgctc cgggacgcgc tcgcgcgcct
      241 cgtcgagccc gacatcgaga tcgacaacga ggaggtgttc gccctccatc gcgtgaacgg
      301 cgggcgggac gtctacttcc tcgtcaacac gaccttcgag ccgcaggcga tgaccgtccg
      361 catgccgggt gagctcgatg cggcggtatg ggacccgtcc tcgggcgagg agcgggtgcc
      421 ggagtcccgc gtggcggacg gccgaaccga gctcgcactc gaactcggtc ccgtcggctc
      481 ggtcgtcatc acgccggagg tgcgtgggac gcgcgccgcg acccccgcgc tcggacggcc
      541 cgccgccacg gtcgccgtgg acggggagtg gcgtttccgt gcggaggatc cgaacgcgct
      601 cgtgctcgac cggtggcgcg ccgcgccgga agcgccgggg gcgtcggccg agggctacgc
      661 ggcgccggac gtcgacgacg acggctggca gccggtcgga cccggcgcgt gggcgttcca
      721 gctgccgagc gagccggacc ggccgtggcc gatcccggtc tggtaccggt tctcgttcgc
      781 gaccgaccgc gtcccgtcgt cactgcatct cgtcgtggac ggcttcgccg gatccgatcg
      841 gcgcgtcttt ctgaatggcg aggaggtcac gaccgagccc gtgcggagcc cgttcgacag
      901 ccagatgaag acgatcgacc tctcgggtca cgtcggcgag ggacggaacg tgctcgcggt
      961 gcggctgacg ctggagtcgt cgaccgacgg ggtcacggat ctgctgaagc tgatcggtgg
     1021 gttcggcgtg tcgcgcggcg acgacggcgt ctatcgcatc gttccgccgg tcgagcgcgt
     1081 gaagcccgcc tcgtggacgg atcagggcta cccgttctac tcgggcgtcg gcgtctactc
     1141 gaccgcggtc gaggttccgc cggggttcgc ggccgggcgc gtgttcctcg aggcgcccgc
     1201 cggagacgac gcgctcgagg tcgtcgtcaa cggccgaagc gcgggcgtgc ggctgtggcc
     1261 gccgtacgcg gcagaagtga ccggcctcct gcgcgacggc gacaacgaga tcgagctccg
     1321 cgtcgcgaac acgctcgcga acctcctgaa cggcgacgag cgggcgtcgg ggctggcgga
     1381 agagccgcgg ctggtggcgt atggatgagc gaggcgaggc ggcgctgaag gttcgcgagc
     1441 tctccgccaa ggcgttcgcg ccgtacggac gcgtgatcga ggcgccgtcg cggccgggag
     1501 acgcctcggg gccggggtgg acgtggtggg ccgagaccgt gctgctttcc ggcgacggcc
     1561 gtccgtgggg cgtggggtac ctgagcctcg agccgaccga tccgcggttc gactgggccg
     1621 agcgccacat gcggacgctt gaggcgatcg tcccgatggc cggcgacgcg ctcgtctacg
     1681 tcggccccgc cgagcatccg gaggaaccgg cgcggctgcc cccgctcgag cggttcgagg
     1741 tgtttcgcgt gccggcgggc acgggcgtcg ttatggatcg tggcgtctgg cacggcgcac
     1801 cgttcgccgc ggacggtccg ggcgctgcga tggtgctgat cctcgaggga acggggcgga
     1861 gcgacgtcac gatcgtgcgc ttcccccccg tggcgttcga gagggagtga ggcagaggtg
     1921 gcggagatcg ttctgaacga cgtgaccaag gtgttcgacg acgggaccgt tgcggtcgcg
     1981 aacctgaacc tgaaggtcga ggacggtctg ttgatggtac tggtgggccc ctcgggctgc
     2041 ggcaagacca cgtcgctacg gatggtcgcg ggtctcgagg agctgaccga cggcgacatc
     2101 cgcatcggcg accgcgtcgt gaacgaggtc gccccgaagg accgcgacat cgcgatggtc
     2161 tttcagaact acgcgctcta cccgcacatg acggtgttcg agaacatggc gttcggcctc
     2221 cgtctccgca agatgcggcg gcagctcgtc cgagagcgcg tcgacgatac ggcgaagacg
     2281 ctcgagctgt tcgagctcct cacgcgcaag cccggtcagc tctcgggcgg tcagcgtcag
     2341 cgggtcgcca tgggccgcgc gatcgtgcgc gagccgaaag cattcctgat ggacgagccg
     2401 ctgtcgaacc tcgacgcgaa gctgcgcgtc cagatgcgct cggagatctc gcggctgcag
     2461 cgcgacgtcg gggtgaccac gctctacgtg acgcacgacc agatcgaggc gatgacgatg
     2521 ggcgacagcg tcgcggttct gaagaagggg ctgctgcagc aggtcgacgc gccgcagaac
     2581 ctgtaccgga agcccgcgac gaacaggttg cgcgggttct cgtagagcac cttgggcgtg
     2641 tcgacctgct gcaggacccc gccgcgcatg accgccaccc ggtcgccgag cgtcatcgcc
     2701 tcggtctggt cgtgggtgac gtagaccgtg gtggtctgca gccgctgctg cagcttcgcc
     2761 accatcgtgc gcatctgcac gcgcagcttg gcgtccaggt tcgacagcgg cgcgtc
//
LOCUS       DASZOB010000418         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29465, whole genome shotgun sequence.
ACCESSION   DASZOB010000418 DASZOB010000000
VERSION     DASZOB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29465"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VG709_06590"
     CDS             complement(<1..195)
                     /locus_tag="VG709_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase"
                     /protein_id="HWC32778.1"
                     /translation="MRATGWRKIATSVWGWPNDPQIYGKIEVDAQPVLDAIAELRGRS
                     GIRVTPTHLVARAVALAARDN"
     gene            complement(192..1382)
                     /locus_tag="VG709_06595"
     CDS             complement(192..1382)
                     /locus_tag="VG709_06595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWC32779.1"
                     /translation="MTTTLDELAARTPATRDRSMDFLRALSILVVVVGHWISGVVFWE
                     GGVIFTRSAIGMTPWLWLLTWFLQVMPIFFFVGGFSNLVTYRSFRRRGDGAGAFLRTR
                     LERLLRPSLAFLGVWAVVQLALHLADVGTPTTPYLRGMKPPGATVPFGPLWFLGMYTI
                     VIVLSPLMIRLHERFGLAVPVAMVAGAIGADALGFATSFEEARWANVAFVWLLPHQLG
                     FFYADGRIQRLSKRAVALIAAGGLAALLLLTNPIFGERGQRLFPGIGHYPKSLLGTDV
                     EPITNTYPPTIALIAMAFWSIGLAMLAREPLQRWLRRTRPWKAVIFVNSVIMTLFLWH
                     MTAFLLAVLTLWPLGLGQQGDTTLAWWAERPLWIAVPGIFLAGLVAVFGRFERARGML
                     PRVG"
     gene            1443..>2113
                     /locus_tag="VG709_06600"
     CDS             1443..>2113
                     /locus_tag="VG709_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWC32780.1"
                     /translation="MSGIDLHTHTVYSDGTFTPAELVRLALERDLDVLAVTDHDSTEG
                     LDEAYAAADGTGLEIVAGVEFSTVSDGHGVHVLAYWVDVANAEFQGELGRLRDDRFRR
                     GERMVERLQELGYPVSFERVREIAKGKNIVRPHVAQALVDAGVVPTVEDAFTRDLIAD
                     GGRAYVEKHALDPIDAIALIKRAGGLAVVAHPGLHEEGLGVPDDLIERMAAAGLDGIE
                     AAHSD"
BASE COUNT          353 a          738 c          754 g          268 t
ORIGIN      
        1 gttgtcgcgt gcggcgagcg cgacggcccg ggcgaccagg tgcgtcggcg tgacgcggat
       61 gccgctccgt ccccggagct ccgcgatcgc gtcgagcacg ggctgggcgt cgacctcgat
      121 cttgccgtag atctgcgggt cgttcggcca gccccagacg ctcgtcgcga tcttccgcca
      181 acccgtcgct ctcatccgac gcgcggaagc atgccacgcg cgcgctcgaa ccgcccgaag
      241 acggcgacga gacccgccag gaagatcccg gggacggcga tccacagcgg acgctccgcc
      301 caccacgcga gcgtggtgtc gccctgctgg ccgagcccga gcggccacag cgtgagcacc
      361 gcgagcagga acgcggtcat gtgccaaagg aacagcgtca tgatcacgct gttcacgaag
      421 atcacggcct tccacggccg cgtgcggcgg agccaccgct ggagcggctc gcgcgcgagc
      481 atcgcgagac cgatcgacca gaacgccatc gcgatgagcg cgatcgtcgg cggataggtg
      541 ttcgtgatcg gctcgacatc ggtgccgagc aggctcttcg ggtagtggcc gatgccgggg
      601 aacagccgct gcccacgctc gccgaagatc gggttcgtga gcagcaagag ggccgcgaga
      661 cccccggccg cgatgagcgc gaccgcgcgc ttcgagaggc gctggattcg cccgtccgcg
      721 tagaagaacc cgagctggtg cggcaggagc cacacgaacg cgacgttcgc ccagcgcgcc
      781 tcctcgaacg acgtcgcgaa cccgagagcg tcggccccga tcgcaccggc gaccatcgcg
      841 acgggcacgg cgagcccgaa gcgctcgtgc agccggatca tcaacgggct cagcacgatc
      901 acgatcgtgt acatcccgag gaaccacagc gggccgaacg ggaccgtcgc gccgggcggc
      961 ttcatgccgc gcaggtaggg cgtcgtcggc gtgccgacgt cggcgaggtg gagcgcgagc
     1021 tgcacgacgg cccagacgcc gaggaacgcg aggctcggcc gcagcagccg ctcgagccgc
     1081 gtccggagga acgcgcccgc gccgtcgccg cggcgccgga acgaccggta cgtgacgagg
     1141 ttcgagaacc cgcctacgaa gaagaagata ggcatgacct ggaggaacca cgtgaggagc
     1201 cacagccacg gcgtcatgcc gatcgcgctc cgcgtgaaga tcacgccgcc ttcccagaaa
     1261 acgacgccgc tgatccagtg cccgacgacg acgacgagga tcgacagcgc gcggaggaag
     1321 tccatcgagc ggtctcgcgt cgccggcgtc cgcgcggcga gctcgtcgag cgtcgtggtc
     1381 acgtggcgtt agaccctccc ggtgagcggt cggtatcgcc cgacctgcta gcgtttggcg
     1441 ccgtgtccgg catcgacctc cacacgcaca ccgtgtactc cgacgggacg ttcacgcccg
     1501 ctgagctcgt gcgcctcgcg ctcgagcgcg acctcgacgt gctcgcggtc acggaccacg
     1561 actcgaccga ggggctcgac gaggcctacg cggccgcgga cggcaccggg ctcgagatcg
     1621 tcgccggagt cgagttctcg acggtgagcg acggccacgg cgtccacgtg ctcgcgtact
     1681 gggtggacgt cgcgaacgcg gagttccagg gcgagctggg gcgcctccgc gacgaccgct
     1741 tccggcgcgg cgagcggatg gtcgagcggc tccaggagct cgggtatccg gtgtcgttcg
     1801 agcgcgtccg cgagatcgcc aaggggaaga acatcgtccg tccgcacgtc gcgcaggcgc
     1861 tggtcgacgc cggcgtcgtt ccgacggtcg aagatgcgtt cacgcgcgac ctcatcgccg
     1921 atggcggacg cgcctacgtc gagaagcacg cgctggatcc gatcgacgcg atcgcgctca
     1981 tcaagcgagc gggcgggttg gccgtcgtcg cgcacccggg cctgcacgag gaggggctgg
     2041 gtgtccccga cgatctcatc gagcgcatgg ccgcggccgg gctcgacggc atcgaggccg
     2101 cacactcgga cca
//
LOCUS       DASZOB010000419         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_150181, whole genome shotgun sequence.
ACCESSION   DASZOB010000419 DASZOB010000000
VERSION     DASZOB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150181"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VG709_06605"
     CDS             <1..1194
                     /locus_tag="VG709_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWC32781.1"
                     /translation="EGAFGYDLSESVAVESPEFLRAAEALTGAPISHGNEAELLINGD
                     QIFPAFLETIASAKRTLCVQTYVYWRGDIARDVAGAIADKAREGVECKVILDALGAAK
                     MDRSLVGQMEDAGVEVVLLRPPKPYAIRRVANRTHRRLLIADGEVGMTGGVGIAAEWT
                     GNAEDPDHWRDTHVRVHGPVVRGMQGAFAENWLEGTGQVLAGDHYLPDLDPIADGGPM
                     QLVRSSATVGDTNVEALYYLAIASARESMDLTAAYFGPRPAFRDALCEAARRGVRVRI
                     VVPGPHIDKGFVRVAGRAAYEELLEAGVRIFEYQPTMLHAKTLVVDGAWSSVGTINFD
                     NRSFQLHDEVTLCVWDERFAGRLGDAFEHDLERSDEIEPGRWNGRAQHQRVTETATKV
                     LRREL"
     gene            1282..>1609
                     /locus_tag="VG709_06610"
     CDS             1282..>1609
                     /locus_tag="VG709_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32782.1"
                     /translation="MATRTVTAPGLDLVRPPEVVLERLAEPAREALVPDAECHLVVQL
                     EGAVVEVDRPHGRPGVVDHERLCVQHRRLVLEDPNPRLEQLLVGGATGDSHEALVDVR
                     AGNEDPH"
BASE COUNT          248 a          563 c          567 g          231 t
ORIGIN      
        1 gagggagcct tcggctacga cctgtcggag tccgtcgcgg tcgagtcgcc cgagttcctg
       61 cgtgccgccg aggcgctgac cggcgcgccg atctcgcacg gcaacgaggc ggagctgctg
      121 atcaacggcg accagatctt ccccgccttc ctcgagacga tcgcctcggc gaagcggacg
      181 ctctgcgtcc agacctacgt ctactggcgc ggcgacatcg cccgcgacgt ggccggggcg
      241 atcgccgaca aggcgaggga gggcgtcgag tgcaaggtca tcctcgacgc gctcggcgcc
      301 gcgaagatgg acaggtcgct ggtcgggcag atggaggacg ccggcgttga ggtcgtgctg
      361 ctgcgccccc caaagcccta tgcgatccgc agggtcgcca accgcacgca ccgccggctg
      421 ctgatcgcag acggcgaggt tgggatgacg ggcggcgtcg gcatcgccgc cgagtggacg
      481 ggcaacgccg aggaccccga tcactggcgc gacacccacg tgcgcgtcca cggtcccgtt
      541 gtgcgcggga tgcagggagc ctttgccgag aactggctcg aggggacggg ccaggtgctc
      601 gcgggcgacc actacctgcc cgacctcgat cccatcgccg acggcggccc gatgcagctc
      661 gtgcgctcct cggcgacggt cggcgacacg aacgtcgagg ccctctacta cctcgcgatc
      721 gcctcggcgc gcgagtcgat ggacctgacc gccgcctact tcgggccgcg gccggcgttc
      781 agggacgcgc tctgcgaggc ggcccgccgc ggagtccggg tgaggatcgt cgtccccggc
      841 ccgcacatcg acaagggctt cgtgcgcgtc gccggtcgcg ccgcctacga ggaactgctc
      901 gaggcagggg ttcgtatctt cgagtaccag ccgacgatgc tgcacgccaa gacgctcgtg
      961 gtcgacggcg cgtggtcgtc ggtcgggacg atcaacttcg acaaccgctc gttccagctg
     1021 cacgacgagg tgacgctctg cgtctgggac gagcgctttg cgggccggct cggcgatgcg
     1081 ttcgagcacg acctcgagcg atccgacgag atcgagcccg ggcgctggaa cggccgggcc
     1141 cagcaccagc gggtgaccga gacggcgacc aaggtcctgc ggcgagagct ctagcccggt
     1201 cgctttgaca cacgccgcgc gcgcggcgat catgaggcgc ggaggcgacc gattccgacc
     1261 gtgccgtgac ctgccccagc tgtggccacc agaaccgtga cggcgcccgg gctcgatctc
     1321 gtccgaccgc ccgaggtcgt gctcgaacgc ctcgccgagc cggcccgcga agcgctcgtc
     1381 ccagacgcag agtgtcacct cgtcgtgcag ctggaaggag cggttgtcga agttgatcgt
     1441 ccccacggac gaccaggcgt cgtcgaccac gagcgtcttt gcgtgcagca tcgtcggctg
     1501 gtactcgaag atccgaaccc ccgcctcgag cagctcctcg taggcggcgc gaccggcgac
     1561 tcgcacgaag cccttgtcga tgtgcgggcc gggaacgagg atcctcacc
//
LOCUS       DASZOB010000420         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_160545, whole genome shotgun sequence.
ACCESSION   DASZOB010000420 DASZOB010000000
VERSION     DASZOB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160545"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VG709_06615"
     CDS             complement(<1..814)
                     /locus_tag="VG709_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWC32783.1"
                     /translation="MPTKLSYSSMNTYERCPLAYRLQYEERVPTKRSSFLAFGDSLHQ
                     ALYRFHDRPVPVAPPVEHLLEILDDVWVSEGYGGEAEEHAYRQHAREVLRRYHEDNAA
                     SYRIPAALEFRFRIEVEGVPVNGVIDRLDRVPGGGYEIVDYKTNRRLPPKASIDRDLQ
                     LSLYALAAREVWGIEPERLTLYYLLPGQRMTTSRSADDLDDLRGRIANVAERIDAGKF
                     EPRENALCGHCEFQHLCPLFRHKFERELSPPRVGEIVEEWIRLKREHQERWRR"
     gene            942..>1983
                     /locus_tag="VG709_06620"
     CDS             942..>1983
                     /locus_tag="VG709_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWC32784.1"
                     /translation="MTERSFVHLHTHTEFSLLDGASRVSDLFEAAQRFGMPAIGMTDH
                     GVLFGALDFFAQAKARGVKPVVGVEAYVAPRSRFDRTPGENDENYRHLTLLARNETGY
                     RNLLRLVTDAHLEGFYHRPRIDKDLLAERAEGLIGFTGCLASEVCRLLLAGQQKKAYE
                     AAAQYRDIFGPGSFYVELQDHGLAEQHQVLPMLVKLAKELGLPLVATNDTHYTEKGHA
                     RAHDVLLCIQQPGKVLADTDRLKFDTEEFYVKSAAEMRELFRDYPEACDATLEIAESC
                     DVDVALGGDLHVPQFEPPDGKGLEGYLHDLVVAGARERYGEPSEEVRQRIEHELRTIN
                     DMGFAGYFVIVWD"
BASE COUNT          312 a          684 c          682 g          305 t
ORIGIN      
        1 gccggcgcca ccgctcctgg tgctcgcgct tcagccggat ccactcctcg acgatctcgc
       61 cgacgcgcgg cggtgacagc tctcgttcga acttgtgccg gaacagcggg cacaggtgct
      121 ggaactcgca gtggccgcac agcgcgttct cgcgcggctc gaacttcccg gcgtctatgc
      181 gctcggcgac gttcgcgatc cgcccccgga ggtcgtcgag gtcgtcggcc gacctcgacg
      241 tcgtcatgcg ctggcccggg aggaggtagt agagcgtgag gcgctccggc tcgatgcccc
      301 agacctcgcg cgccgcgagc gcgtacagcg agagctggag gtcgcggtcg atcgacgcct
      361 tcggcggcag ccggcggttc gtcttgtagt cgacgatctc gtagccgcca cccggcacgc
      421 ggtcgagcct gtcgatgacg ccgttcaccg ggacgccctc gacctcgatc cggaagcgga
      481 actcgagcgc cgcggggatc cggtacgacg cggcgttgtc ctcgtggtac cgccgcagca
      541 cctcgcgcgc gtgctggcgg taggcgtgct cctcggcctc gccgccgtag ccctcggaca
      601 cccacacgtc gtcgaggatc tcgagcagat gctccaccgg cggcgccacg ggaacaggcc
      661 ggtcgtggaa ccggtagagc gcctggtgga gcgagtcccc gaacgccagg aacgaggacc
      721 gcttcgtcgg cacccgctcc tcgtactgga gccggtaggc gagcgggcac cgctcgtagg
      781 tgttcatcga ggagtacgag agcttcgtcg gcaccgtgac ctgcgctcct tgacgccttc
      841 cctcgccggg agtatgaatc tctctcggcg gaatttcaag attgtagttc tccacgggtt
      901 ttccacaggg cgggtcggca gcgggaggta cggtcccggg catgacggaa cggagcttcg
      961 tccacctcca cacccacacg gagttctcgc tgctcgacgg cgcctcgagg gtctcggacc
     1021 tcttcgaggc ggcccagcgc ttcgggatgc cggccatcgg gatgaccgac cacggggtgc
     1081 tgttcggggc gctcgacttc ttcgcgcagg cgaaggcgcg cggcgtgaag cccgtcgtgg
     1141 gcgtcgaggc gtacgtcgcg ccgaggtcac gcttcgaccg gacgcccggc gagaacgacg
     1201 agaactaccg ccacctgacg ctcctcgccc gcaacgagac ggggtaccgg aacctcctgc
     1261 ggctcgtcac cgacgcgcac ctcgagggct tctaccaccg cccccggatc gacaaggacc
     1321 tcttggccga gcgcgccgag gggctgatcg ggttcacggg atgcctcgcg agcgaggtct
     1381 gccggctgct cctcgccggc cagcagaaga aggcgtacga ggcggcggcg cagtaccgcg
     1441 acatcttcgg cccggggtcc ttctacgtgg agctccagga ccacgggctg gcggagcagc
     1501 atcaggtcct cccgatgctc gtgaagctcg cgaaagagct cgggctgccg ctcgtcgcga
     1561 ccaacgacac ccattacacc gagaaggggc acgcccgcgc gcacgacgtc ctgctgtgca
     1621 tccagcagcc cgggaaggtg ctcgccgaca ccgaccggct caagttcgac accgaggagt
     1681 tctacgtgaa gtcggcggcc gagatgcgag agctcttccg cgactacccg gaggcgtgcg
     1741 acgcgacgct cgagatcgcc gagtcgtgcg acgtcgacgt cgctctgggc ggcgacctac
     1801 acgtgccgca gttcgagcca ccggatggca aggggctcga ggggtacctc cacgacctcg
     1861 tcgtcgccgg cgcacgcgag cggtacggcg agccgtcgga ggaggtcagg cagcgcatcg
     1921 aacacgagct ccgaacgatc aacgacatgg ggttcgccgg ctacttcgtg atcgtctggg
     1981 acc
//
LOCUS       DASZOB010000421         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29619, whole genome shotgun sequence.
ACCESSION   DASZOB010000421 DASZOB010000000
VERSION     DASZOB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29619"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..254)
                     /locus_tag="VG709_06625"
     CDS             complement(39..254)
                     /locus_tag="VG709_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32785.1"
                     /translation="MRGNERTPMRIACDECAWEGTDRCGDCVVSFIVDREDEDPVVGD
                     ADEARAMRRRGEAGLVPLLRLERREAG"
     gene            complement(293..1468)
                     /locus_tag="VG709_06630"
     CDS             complement(293..1468)
                     /locus_tag="VG709_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWC32786.1"
                     /translation="MASSAPLPLEGTVVLDLTRLLPGNYATLLLADLGADVVKVEEPG
                     RGDYIRWMPPMVDGDGAIHRALNRNKRSLTLNLKDPEGVRLLRRLAEGADALVESFRP
                     GVLDRLGAGYEALAEANERLVYCAITGFGQDGPYRDRVGHDINYIGYAGLLDATGTPD
                     GPPVLPSVQVGDFGGGMAAALGLAAAVADARRSGRGRFVDVSMLDVVTSWLGVLSSWY
                     LAMGTPPPRGGMPLTGGMAWYRVYRTRDGRHLAVGAIEERFWRALCDELGVPDLADEQ
                     YAPRERQEEIAARLGAIFASMDRDEWVRRLAPLEACVGPVNDVGEAMEDPQIVHRGTV
                     ARVNGVPVGPGPAIKVSATDPKEPAAAPALGEHTGEILRSLGLDEEQISDLRAHRIV"
     gene            complement(1458..1682)
                     /locus_tag="VG709_06635"
     CDS             complement(1458..1682)
                     /locus_tag="VG709_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32787.1"
                     /translation="MRAGRRTRFRCGQCGNLTRFDVVETRRTRAYYHFTLGGDLTVED
                     EEVLATERERVTCRWCGSSERIEELPVDGE"
     gene            complement(1679..2170)
                     /locus_tag="VG709_06640"
     CDS             complement(1679..2170)
                     /locus_tag="VG709_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32788.1"
                     /translation="MPTGSTDALAPNEAMPWIGSGPIYACNERSAGTLVEALRRLGFD
                     VVELEGERIVNHESFTDLFTYAFGFPAASANGWQSLAEVPTERLTRPPGERVAIVWRR
                     ADHSATFCLKCVVEACAFLLGWGRALERSDAVPTRRLDVFLLGQTRDFAQPPPGWTLE
                     GIA"
     gene            2222..3841
                     /locus_tag="VG709_06645"
     CDS             2222..3841
                     /locus_tag="VG709_06645"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HWC32789.1"
                     /translation="MQRSFDDVGVPLSEVTFACVDLETTGGAPPDARITEVGAVKYRG
                     GERLGTFHTLVDPGVPIPRFVTYLTGIDERAVAGAPPIEAVIPSLREFLRGTVLVAHN
                     AAFDARFLAFELDRLGYEPLEGAPVCTAKLARRVVADDVANVRLETLAQFFRTAARPC
                     HRALEDAEACADVLHSLLDLGGRLGILTLGDLREAVRARGHSHYRKIRLADALPSAAG
                     VYVFRREPTASGAGDVLYVGKSKDIRARVRSYFYGDDRKKIEALLAETGAVEARPCET
                     ELEALVLEARLIRRHQPKYNRRGKAWRRYAYLKLDTAEAWPRLKVVRRANGDGAFLGP
                     FASAARARLAKEALEDVFAIRRCTASMGRRTRFAPCALGDLGRCLAPCDGRVTPERYE
                     ELVRSLRHALSSPGGLLEALAPRMASLAEAERVEEAASVRDRTEALVAALARARSERW
                     LIDAGRLEVEVAGRSVSFTGGALVGRAGGDGFSWPPPLEAADEVRAATSWLASNRPRV
                     VAADRPPSEPVAGGLSLATLSSRFRARERTR"
     gene            3860..>4499
                     /locus_tag="VG709_06650"
     CDS             3860..>4499
                     /locus_tag="VG709_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWC32790.1"
                     /translation="MERAVIVEGARTPVGKFLGSFADVPAVDLGIIATREALRRAGVE
                     ASEVDEVVFGHARQAGNGPNTARQVGYRSGIPQEVPAYTVNMACGSGTKAVEHAAGSI
                     ALGNAEVVVAGGMENMTRTPFILDRMRFGYRMGNAPAQDGMYRDGFLDPLCGLVMGET
                     AENLARKYDIPREEQDDFALATQRKAEAGKARRAKEIVAVEVPAKKGETRTVV"
BASE COUNT          601 a         1676 c         1605 g          617 t
ORIGIN      
        1 attgtcgcgc tcgcggaggg aggtggcaat gaacgacctc atcccgcctc cctgcgctcg
       61 agccggagga gggggaccag accggcctcg ccccgccgcc gcatcgccct cgcctcgtcg
      121 gcgtcgccca ccacggggtc ctcgtcctcg cggtcgacga tgaacgagac gacacagtcg
      181 ccgcaccggt cggtgccctc ccacgcgcac tcgtcgcacg cgatccgcat cggggttcgt
      241 tcgttccctc gcatggcggc accgtagcga ccccctctga cacgcccgcc gcttacacga
      301 tgcggtgcgc ccgcaggtcc gagatctgct cctcgtcgag ccccaacgac cggaggatct
      361 cgcccgtgtg ctccccgagc gccggcgcgg ccgcgggctc cttggggtcg gtcgcggaca
      421 ccttgatcgc cggccccggt cccaccggga cgccgttcac gcgcgcgacg gtcccacggt
      481 gaacgatctg gggatcctcc atcgcctcgc ccacgtcgtt caccgggccg acgcacgcct
      541 cgagcggcgc caggcggcgc acccactcgt cacggtccat ggaggcgaag atcgcgccga
      601 gccgcgccgc gatctcctcc tggcgctcgc gaggcgcgta ctgctcgtcg gccaggtcgg
      661 ggacgccgag ctcgtcgcag agcgcgcgcc agaagcgctc ctcgatcgcg ccgaccgcga
      721 ggtgcctccc gtcgcgggtg cggtacacgc ggtaccaggc catccctccg gtgagcggca
      781 tcccgccccg cggcggcggc gttcccatcg cgaggtacca cgaggagagc acgccgagcc
      841 acgaggtcac gacgtcgagc atcgagacgt cgacgaaccg gcctcgcccg ctccgccgcg
      901 cgtcggccac ggcggccgcg agcccgagcg ccgccgccat cccgccgccg aagtccccga
      961 cctgcaccga aggcaggacc ggcgggccgt ccggcgtgcc cgtcgcgtcg agcagccccg
     1021 cgtacccgat gtagttgatg tcgtggccga cgcggtcgcg atacgggccg tcctggccga
     1081 agcctgtgat cgcgcagtac acgagccgct cgttcgcctc cgcgagcgcc tcatagccgg
     1141 cgccgagccg gtcgaggacg cccggacgga acgattcgac cagcgcgtca gcgccttcgg
     1201 cgagccggcg tagcagacgc acgccctcgg ggtccttcag gttcagcgtg agcgagcgct
     1261 tgttgcggtt gagcgcccgg tggatcgccc cgtctccgtc caccatcggc ggcatccagc
     1321 ggatgtagtc gccgcgcccc ggctcctcga ccttgaccac gtcggccccg agatccgcga
     1381 ggagcagcgt cgcgtagttg cccgggagga gcctcgtcag atcgaggacg acggttccct
     1441 cgagcggaag cggggcgcta ctcgccatcg accggcagct cctctatccg ctccgaggag
     1501 ccgcaccacc ggcaggtgac tcgctcccgc tcggtcgcga gcacctcctc gtcttcgacc
     1561 gtgaggtcgc cgccgagcgt gaagtggtag taggcgcggg tccgtctggt ctcgacgacg
     1621 tcgaaccgcg tgaggttccc gcattgcccg cagcggaacc gcgtccgtcg gccggccctc
     1681 atgcgatgcc ctcgagcgtc cacccgggcg ggggctgcgc gaagtcgcgg gtctgaccga
     1741 gcaggaacac gtcgagacgg cgcgtcggga cggcgtccga gcgctcgagc gcgcgccccc
     1801 agccgaggag gaaggcgcat gcctcgacga cgcacttcag gcagaacgtg gccgagtggt
     1861 cggcgcgacg ccacacgatc gcgacgcgct ctccgggcgg cctcgtgagc cgctcggtgg
     1921 ggacctcggc gagcgactgc cagccgttcg cggaggcggc cgggaacccg aacgcgtacg
     1981 tgaagaggtc cgtgaacgac tcgtggttga cgatccgctc gccctcgagc tcgacgacgt
     2041 cgaacccgag ccggcggagc gcctcgacga gcgtaccggc ggagcgctcg ttgcacgcgt
     2101 agatcgggcc actcccgatc cacggcatgg cctcgttcgg cgcgagggcg tcggtcgacc
     2161 cggtcggcac ccggtgattc tcccccaagc cgtgtcagac ccggtggcta gcgtctgacc
     2221 cgtgcagcgc tcgttcgacg acgtgggcgt gccgctctcc gaggtgacgt tcgcctgcgt
     2281 cgacctcgag acgaccggcg gcgcgccgcc cgacgcgcgg atcaccgagg tcggcgccgt
     2341 gaaataccgg ggcggcgagc gcctcggcac cttccatacg ctcgtcgatc ccggcgtccc
     2401 gatcccgcgg ttcgtgacgt acctcaccgg catcgacgaa cgggccgtcg cgggtgcacc
     2461 gccgatcgag gccgtgattc cctcgctccg cgagttcctc agggggacgg tcctcgtcgc
     2521 gcacaacgcc gcgttcgacg cgcgcttcct cgcgttcgag ctcgaccgcc tcggctacga
     2581 gccgctcgag ggagcgccgg tgtgcacggc gaagctcgcc cggcgggtcg tcgcggacga
     2641 cgtcgcgaac gtgcgcctcg agacgctggc gcagttcttc cgcacggcgg cgcgcccgtg
     2701 tcaccgcgcc ctcgaggacg ccgaggcgtg cgccgacgtc ctccactcgc tgctcgatct
     2761 cggaggacgc ctcggcatcc tcacgctcgg ggacctccgt gaggcggtcc gggcgcgcgg
     2821 ccactcccat taccggaaga tccgcctcgc cgacgcgctg ccctctgccg cgggggtgta
     2881 cgtgttccgg cgcgagccga cggcgtcggg cgccggcgac gtgctgtacg tcggcaagtc
     2941 gaaggacatc cgggcgcggg tccgctcgta cttctacggc gacgatcgga agaagatcga
     3001 ggcgctgctc gccgagacgg gtgccgtcga ggcgcgtccg tgcgagacgg agctcgaggc
     3061 cctcgtgctc gaggcgaggc tcatccgccg ccaccagccg aagtacaacc gccgcggcaa
     3121 ggcctggcgg cggtacgcct acctcaagct cgacaccgcc gaggcgtggc cgcgcctcaa
     3181 ggtcgtacgg cgtgcgaacg gcgacggcgc cttcctcggc ccgttcgcca gcgcggcccg
     3241 cgcccggctc gcgaaggagg cgctcgagga cgtgttcgcg atccgccgct gtacggcctc
     3301 gatgggtcgg cgcacacggt tcgcgccgtg cgcgctcggc gatctcgggc gctgcctggc
     3361 gccctgcgac ggccgcgtca cgcccgagcg ctacgaggag ctcgtccggt ccctccgcca
     3421 cgccctctcc tcgccgggcg ggctcctcga ggcgctcgcg ccgcgcatgg cctcgctcgc
     3481 ggaggcggag cgggtcgagg aggcggcctc ggtacgggac cgcaccgagg cgctcgtcgc
     3541 cgcgctcgcg cgtgcccgct ccgagcggtg gctgatcgac gccggccgtc tcgaggtcga
     3601 ggtcgccggg cgaagcgtct cgttcaccgg cggtgccctc gtgggccgtg ccggcggtga
     3661 cgggttctcg tggcctcctc cgctcgaggc cgccgacgag gtccgcgccg caacgtcgtg
     3721 gctcgcgtcg aatcgcccgc gcgtcgtcgc ggccgaccgg cctccctccg agccggtcgc
     3781 cggaggcctg agcctggcga cgctctcgag caggttccgc gcgcgagagc ggacacgctg
     3841 acctcgctac actggtcgtg tggagcgggc cgtcatcgtc gagggcgcgc ggacgccggt
     3901 cgggaagttc ctcggctcgt tcgccgacgt ccccgccgtc gatctcggca tcatcgcgac
     3961 gcgggaggcg ctccggcgcg ccggcgtcga ggcttcggag gtcgacgagg tcgtcttcgg
     4021 ccacgcgagg caagcgggga acggaccgaa caccgcgcgc caggtcggct atcgctccgg
     4081 catcccgcag gaggtgccgg cgtacaccgt caacatggcg tgcggctccg gcacgaaggc
     4141 cgtggagcac gccgccggct cgatcgcgct cgggaacgcg gaggtcgtcg tcgcgggcgg
     4201 catggagaac atgacgcgga cgccgttcat cctcgaccgg atgcggttcg gttaccgcat
     4261 ggggaacgcg ccggcccagg acgggatgta ccgagacggc ttcctcgacc cgctctgcgg
     4321 cctcgtgatg ggtgagaccg ccgagaacct cgcgcggaag tacgacatcc cgcgggagga
     4381 gcaggacgat ttcgcgctcg cgacgcagcg gaaggccgag gccggcaagg cccgccgcgc
     4441 gaaggagatc gtggcggtcg aggtccccgc gaagaagggc gagacgcgca ccgtcgtgg
//
LOCUS       DASZOB010000422         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_79752, whole genome shotgun sequence.
ACCESSION   DASZOB010000422 DASZOB010000000
VERSION     DASZOB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79752"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..560
                     /locus_tag="VG709_06655"
     CDS             81..560
                     /locus_tag="VG709_06655"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HWC32791.1"
                     /translation="MSDATVAVVAIAAAGAAALALVLALAFYVKLRRVRRAQAVLLGS
                     GKDDLVDFAVSLQARIDDVNRAVDEVAGALSRLDRRIDACLSHTSIVRYDAYQDAGGH
                     QSASLAFLDAVRSGIVVSAIQGRDYARIYVKELDRGRPSVALSPEEQEAVERAMAVG"
     gene            567..974
                     /locus_tag="VG709_06660"
     CDS             567..974
                     /locus_tag="VG709_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32792.1"
                     /translation="MPYRKAEIRPLRPDDEPLLFGLASIDRGADERTLEVLERETVFV
                     AEVAGSPAGYVALVPEEERVRVDQLFVSPEHEREGVGRQLLEYAEGWAISRRARALRA
                     VVAEHDRRATAFYRDRGFVPVEPDVVELVLPEP"
     gene            1006..1515
                     /locus_tag="VG709_06665"
     CDS             1006..1515
                     /locus_tag="VG709_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWC32793.1"
                     /translation="MGQVLVLHASYEPLNVCSVRRAHVLVFKGKAEVVEHLGRPLRSA
                     AERFLWPHVIRLLQYVRVPKSVQRKISRRALFARDGWRCQYCGNEGGKLTLDHVVPRS
                     RGGESVWENVVTSCAPCNLRKGNRTAEEAGMRLHAAPREPAPVLFVTLSAPRIPDGWQ
                     PYLSAYAAA"
BASE COUNT          228 a          576 c          571 g          225 t
ORIGIN      
        1 ccgtggaacc ggtgcccgga gaggagaaca cgaacaacaa caccgccgag tacgtcgtgt
       61 tcttcacggt cggctaggcc gtgtccgacg ccaccgttgc ggtcgtcgcg atcgccgccg
      121 cgggcgccgc ggcgcttgcg ctcgtactcg cgctcgcctt ctacgtcaag ctccgccgcg
      181 tccggcgcgc ccaggccgtc ctcctcggga gcggaaagga cgacctcgtc gacttcgccg
      241 tctcgctcca ggcgcgaatc gacgacgtga accgcgccgt cgacgaggtc gcaggcgcgc
      301 tctcgcggct cgaccggcga atcgacgcct gcctgtcgca cacctcgatc gtccgctacg
      361 acgcctacca ggacgcgggc ggccaccagt ccgcgtcgct cgcgttcctc gacgccgtcc
      421 ggtccggcat cgtcgtgagc gcaatccaag gccgggacta cgcaaggatc tacgtgaagg
      481 agctcgaccg cggccgtccg tccgtcgccc tgtccccgga ggagcaggag gcggtcgagc
      541 gggcgatggc ggtcggctga gacgggatgc cgtaccggaa ggcggagata cggcctctgc
      601 gtccggacga cgagccgctg ctcttcggcc tggcgagcat cgaccgcggc gccgacgagc
      661 gcacgctcga ggtcctcgag cgcgagaccg tcttcgtcgc cgaggtggcg ggctcgccgg
      721 cggggtacgt cgcgctcgtg cccgaggaag agcgcgtccg cgtcgaccag ctcttcgtga
      781 gccccgagca cgagagggaa ggcgtcggcc ggcagctcct cgagtacgcc gagggctggg
      841 ccatctcgcg gcgggcccgg gcgctgcgcg cagtcgtcgc cgagcacgac cggcgcgcga
      901 cggcgttcta ccgcgaccgg ggattcgtgc cggtcgagcc cgacgtcgtc gagctcgtgc
      961 ttccggagcc gtagccgcga cgcgccgcgg tacgattttc acgccatggg acaggtcctc
     1021 gtcctgcacg cgagctacga gccgctcaac gtctgcagcg tccggcgtgc gcacgtgctc
     1081 gtcttcaagg gcaaggccga ggtcgtcgag catctggggc ggccgctccg ctcggccgcc
     1141 gagaggttcc tgtggccgca cgtcatccgg ctcctccagt acgtccgcgt gccgaagtcg
     1201 gtgcagcgga agatctcgcg tcgcgcgctc ttcgcccggg acggctggcg ctgccagtac
     1261 tgcgggaacg agggcgggaa gctcacgctg gaccacgtgg tgccacgctc gcgcggaggc
     1321 gagtccgtct gggagaacgt ggtgacgtcg tgcgcgccgt gcaacctccg gaagggaaac
     1381 cgcacggcag aggaggccgg gatgcgcctg cacgccgccc cgcgcgagcc cgcgcccgtc
     1441 cttttcgtca cgctctccgc gccacggatt cccgacggct ggcagccgta cctctcggcg
     1501 tacgcagcgg cctgacgccg cggagaaacc gcgccgaggt cgctcggaac atcgcgactc
     1561 gccgcgcggt ccaagcggaa agcccccgag gaggtggagc
//
LOCUS       DASZOB010000423         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_89803, whole genome shotgun sequence.
ACCESSION   DASZOB010000423 DASZOB010000000
VERSION     DASZOB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89803"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /gene="rplO"
                     /locus_tag="VG709_06670"
     CDS             complement(<1..367)
                     /gene="rplO"
                     /locus_tag="VG709_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWC32794.1"
                     /translation="MKLHHLRPGPGAKRDRRRVGRGRAAGQGKTAGRGTKGYLARNPK
                     RTGYEGGQMPLQRRVPKRKGFTPRNREEWAVVNVERLAKRFDAATTISPDAMADAGLV
                     RRRLPIKVLGRGEIDRAIVV"
     gene            complement(364..549)
                     /gene="rpmD"
                     /locus_tag="VG709_06675"
     CDS             complement(364..549)
                     /gene="rpmD"
                     /locus_tag="VG709_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWC32795.1"
                     /translation="MARLRVTQVRSVVGTPKRQRDTVRALGLKRIRHTVEQEDRPEIR
                     GMLRRVSHLVRVEEVAS"
     gene            complement(536..1129)
                     /gene="rpsE"
                     /locus_tag="VG709_06680"
     CDS             complement(536..1129)
                     /gene="rpsE"
                     /locus_tag="VG709_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWC32796.1"
                     /translation="MRRYDSSDRGPYEERVISINRVAKVVKGGRRFSFAALVVIGDGA
                     GRVGMGYGKAKEVPAAIQKGVEEARKAMFDIPLMGTTIPHEVLGQDGAGRVLLKPAAP
                     GTGVIAGGPVRAVVECAGIKDILSKSLGSGNPINIVHATIAGLRQLRRPEDVAKLRGK
                     ELQEIAPRPMLDAMADARDRANQTRVGLEGAEAGGEA"
     gene            complement(1126..1476)
                     /gene="rplR"
                     /locus_tag="VG709_06685"
     CDS             complement(1126..1476)
                     /gene="rplR"
                     /locus_tag="VG709_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWC32797.1"
                     /translation="MNAATKRAALVRRHRRVRRKVSGDGVRPRLSVYRSNRQIYAQLI
                     DDLSGRTIVAASSLDTGASGKKTDAARAVGELIARRAREAGIERAVLDRGGRLYHGRV
                     RALAEGARDGGLQI"
     gene            complement(1473..>1559)
                     /locus_tag="VG709_06690"
     CDS             complement(1473..>1559)
                     /locus_tag="VG709_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWC32798.1"
                     /translation="EPYKGKGIRYEGEYVRKKAGKAAKGSLG"
BASE COUNT          188 a          613 c          512 g          246 t
ORIGIN      
        1 gcaccacgat cgcgcggtcg atctctccgc gcccgaggac cttgatcggg agccgtcggc
       61 ggacgagccc ggcgtcggcc atcgcgtcgg gcgagatcgt cgtcgcggcg tcgaagcgct
      121 tcgccagacg ctcgacgttc acgaccgccc actcttcgcg gttccgcggc gtgaacccct
      181 tgcgcttcgg gacccggcgc tggagcggca tctggccgcc ctcgtagccg gtccgcttcg
      241 ggttccgcgc gaggtagccc ttcgtcccgc gcccggcggt ctttccctgg cccgccgcgc
      301 ggccgcgccc cacacgccgg cgatcgcgct tcgcgccggg gcccggacgg agatggtgaa
      361 gcttcacgac gccacctcct ccacgcgcac gaggtgggac accctgcgga gcatcccccg
      421 gatctccgga cggtcctcct gctcgaccgt gtgccggatg cgcttgagcc ccagcgccct
      481 gacggtgtcg cgctggcgct tcggcgtccc gacgacgctc cggacctgcg tgaccctaag
      541 cctcgccacc ggcctctgcc ccttccaggc ccacccgcgt ctggttcgcg cggtcccgcg
      601 cgtcggccat cgcgtcgagc atcgggcgcg gcgcaatctc ctggagttcc ttcccgcgca
      661 gcttcgcgac gtcctcggga cgccggagct gccgcaggcc ggcgatcgtc gcgtggacga
      721 tgttgatcgg attgccggat cccagcgact tcgacaggat gtccttgatc ccggcgcact
      781 cgacgaccgc gcggaccgga ccgccggcga tgacaccggt ccccggcgcc gcgggcttga
      841 gcagcacacg cccggcgccg tcctgcccga gcacctcgtg agggatcgtc gtccccatga
      901 gcgggatgtc gaacatcgcc ttccgcgcct cctcgacgcc cttctggatc gcggcgggga
      961 cctccttcgc cttgccgtac cccatgccga cgcgtccggc gccgtcgccg atcacgacga
     1021 gcgcggcgaa cgagaaccgc cgcccgccct tgacgacctt ggcgacgcgg ttgatcgaga
     1081 tgacgcgctc ctcgtaggga ccgcggtcgg agctgtcgta gcgcctcaaa tctggagtcc
     1141 tccgtcgcgc gcgccctcgg cgagggcacg gacccgccca tggtacaggc gccctccccg
     1201 atcgagcacc gcgcgctcga tgccggcctc gcgcgccctc cgcgcgatca gctcgccgac
     1261 ggcgcgtgcg gcgtcggtct tcttgcccga ggcaccggtg tcgagcgacg acgccgccac
     1321 gatcgttcga cccgacaggt cgtcgatgag ctgcgcatag atctgccggt tcgagcggta
     1381 caccgagagc cgcggtcgca ccccgtcgcc ggagaccttc cggcgcacgc ggcgatgacg
     1441 gcgaacgagc gcggcgcgct tcgtggcggc gttcatccga gcgacccctt cgcggccttg
     1501 ccggccttct ttcggacgta ctcgccctcg tagcggatcc ccttgccctt gtacggctc
//
LOCUS       DASZOB010000424         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_176012, whole genome shotgun sequence.
ACCESSION   DASZOB010000424 DASZOB010000000
VERSION     DASZOB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176012"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VG709_06695"
     CDS             complement(<1..1412)
                     /locus_tag="VG709_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HWC32799.1"
                     /translation="MAPVEERKEVRPPERRGGILARLPFRPSPTGDGAATGIAGLLRK
                     LKADKADAKLVQRVYEFAEDSHRGQKRKSGEDFIAHPLEVAHLLADLGMDTTTIAAGL
                     LHDVVEDTELSLEEVERRFGSEIAALVDGVTKLDKITYRSREAEQAENTRKMIIAMAR
                     DIRVLIIKLADRLHNMRTLDSLAPEKQKAKATETLEIYGPLAHRLGIHQLKWELEDLA
                     FQTLHPKRFDEIQNLVGKRGEERAAYLETVRDQVQAKLRESKLKADVAGRPKHLYSVY
                     EKMVLRGKEFSEIYDLVGIRVLVDSVRDCYAALGAIHSLWKPVPGRFKDYIAMPKFNM
                     YQSLHTTVIGPEGRPLEIQIRTHSMHRTAEFGIAAHWRYKDDDTKSEESDLAWLAQML
                     EWQRELQDPEEFMESLKIDLYRGQVFVYTPKGDVVSLAAGATPIDFAYTIHTEVGHRC
                     IGARVSGKLVPLDYKLQTGDT"
BASE COUNT          211 a          528 c          549 g          281 t
ORIGIN      
        1 gtgtcgccgg tctgcagctt gtagtcgagc ggcacgagct tcccggacac gcgcgcgccg
       61 atgcagcggt gcccgacctc ggtgtggatg gtgtaggcga agtcgatcgg cgtcgcgccg
      121 gccgccagcg acacgacatc gcccttcggc gtgtacacga agacctgacc ccggtagagg
      181 tcgatcttca ggctctccat gaactcctcg gggtcctgga gctcgcgttg ccactcgagc
      241 atctgcgcga gccacgcgag gtcgctctcc tcggacttgg tgtcgtcgtc cttgtagcgc
      301 cagtgcgcgg cgatcccgaa ctcggccgtc cggtgcatcg agtgcgtccg gatctggatc
      361 tcgagcgggc ggccctcggg cccgatcacc gtcgtatgga gcgactggta catgttgaac
      421 ttgggcatcg cgatgtagtc cttgaagcgg cccggaaccg gcttccagag cgagtggatc
      481 gcgccgagcg ccgcgtagca gtcgcggacg gagtcgacca gcacgcggat cccgaccagg
      541 tcgtagatct cactgaactc cttgccgcgc agcaccatct tctcgtagac gctgtagagg
      601 tgcttcgggc ggccggcgac gtcggccttg agcttcgact cgcgcagctt ggcctgcacc
      661 tggtcgcgga cggtctcgag gtacgcggcg cgctcctctc cccgcttgcc gacgaggttc
      721 tggatctcgt cgaagcgctt ggggtgcagc gtctggaacg cgaggtcctc gagctcccac
      781 ttcagctggt ggatgccgag gcggtgcgcg agcggcccgt agatctcgag ggtctcggtc
      841 gccttggcct tctgcttctc cggggcgagg ctgtcgagcg tccgcatgtt gtggagccgg
      901 tcggccagct tgatgatcag cacgcggata tcgcgcgcca tcgcgatgat catctttcgc
      961 gtgttctcgg cctgctcggc ctcgcgcgag cggtaggtga tcttgtcgag cttcgtgacg
     1021 ccgtcgacga gcgcggcgat ctccgagccg aaccgacgct cgacctcctc gagcgagagc
     1081 tccgtgtcct cgacgacgtc gtggaggagc ccggcggcga tcgtggtggt gtccatgccg
     1141 aggtccgcga gcaggtgcgc gacctcgagc gggtgggcga tgaagtcctc gccgctcttg
     1201 cgcttctgac cgcggtggga gtcctcggcg aactcgtaga cccgctggac cagcttcgcg
     1261 tcggccttgt ccgccttcag cttccggagg agcccggcga tcccggtcgc ggcgccgtcg
     1321 cccgtgggcg aggggcggaa cggaaggcgc gcgaggatgc cgccgcgccg ttcgggcggc
     1381 cggacctcct tgcgctcctc gacgggggcc acctcacccc cattatgcag gcgcgacggc
     1441 gtctcccaag cggatcggcc cgcctcggac gatgtcggcg cggagcccgc cgcgatgcac
     1501 gagcgccttc ctgacgccgg gagcgccgga cagacgctcc atgtgcccgc acggctcgca
     1561 gagccggac
//
LOCUS       DASZOB010000425         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29674, whole genome shotgun sequence.
ACCESSION   DASZOB010000425 DASZOB010000000
VERSION     DASZOB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29674"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1648)
                     /gene="dnaE"
                     /locus_tag="VG709_06700"
     CDS             complement(<1..>1648)
                     /gene="dnaE"
                     /locus_tag="VG709_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWC32800.1"
                     /translation="PGRGSAAGSVVSSCLRITDLDPLRYGLIFERFLNPERKQMPDID
                     MDFDERRRDEVIRYVADRYGHDHVAQIITFQTIKGKQGIRDAARVLGYPASIGDRLCK
                     MYPPAVLGREHPIEEALKESRELREAYEREPEAKEIVDAARQLEGLRREDSVHAAGVV
                     IGDAPLVKYMPLKLSKDSRDDSKRVVTQFDMHGVEDLGLLKMDFLGLRNLSVIEDTLG
                     MLREQGVSLDIDHVPLDDQKTYEMLRSGDTTGVFQLESPGMRSLIRDLAPDRFEDLMA
                     LVSLYRPGPLNAGMHIEYAERKHGRKPVEYPHADLEPILERTYGIIVYQEQVMEIAVR
                     MAGYSMGEADLLRKAMGKKIREQLIPHREKFVNGATSYRYDRRLAEHIFDLIVPFADY
                     GFNASHACGYAFIAYQTAYLKAHHPVEYMAALLTSVKDDKDKKPFYLNACRLMGIRVL
                     PPDVNESHIEFTPAGEEIRYGLSAVRNVGAGAVAQIIEARQHKGAFTSFSDFCRKVDP
                     AILHKKVLESLVLSGAFDSLGYKRRGLFDGFSKVCEPIVSQ"
BASE COUNT          252 a          537 c          565 g          294 t
ORIGIN      
        1 ctgcgagacg atcggctcgc acaccttcga gaacccatcg aagagcccgc gccgcttgta
       61 tccgagcgag tcgaacgcgc cggacagcac gaggctctcg agcaccttct tgtgcaggat
      121 cgcggggtcg accttccggc agaagtcgga gaacgaggtg aacgcgccct tgtgctggcg
      181 cgcctcgatg atctgcgcga cggcgccggc gccgacgttg cgcaccgccg agaggccgta
      241 acggatctcc tcgccggccg gcgtgaactc gatgtgggac tcgttgacgt cgggcggcag
      301 cacccggatg cccatcagcc ggcatgcgtt caggtagaag ggcttcttgt ccttgtcgtc
      361 cttgacgctc gtcagcaacg ccgccatgta ctcgaccggg tgatgcgcct tgaggtacgc
      421 ggtctggtac gcgatgaacg cgtagccgca ggcgtgcgac gcgttgaagc cgtagtcggc
      481 gaacggcacg atgaggtcga agatgtgctc ggctagccgc cggtcgtacc gatacgacgt
      541 cgcgccgttc acgaacttct cgcggtgcgg gatcagctgc tcgcggatct tcttgcccat
      601 ggccttacgg aggaggtcgg cctcgcccat cgagtagccg gccatccgca cggcgatctc
      661 catcacctgt tcctggtaga cgatgatccc gtacgtccgt tcgaggatcg gctcgaggtc
      721 cgcgtgcggg tactcgacgg gcttgcgccc gtgcttgcgc tcggcgtact cgatgtgcat
      781 gccggcgttg agcggccccg gccggtagag cgagacgagc gccatgaggt cctcgaagcg
      841 gtccggcgcg aggtcgcgga tcagcgaccg catcccgggg ctctcgagct ggaatacgcc
      901 ggtcgtgtcg ccgctgcgca gcatctcgta cgtcttctgg tcgtcgagcg ggacgtggtc
      961 gatgtcgagt gagacgccct gctcgcggag catcccgagc gtgtcctcga tcaccgagag
     1021 gttccgaagc ccgaggaagt ccatcttgag gaggccgagg tcctcgacgc cgtgcatgtc
     1081 gaactgcgtg accacgcgct tcgagtcgtc gcgcgaatct ttcgagagct tgagcggcat
     1141 gtacttgacg agcggcgcgt cgccgatcac gacgccggcg gcgtggacgg agtcctcgcg
     1201 gcggagcccc tcgagctgac gggccgcgtc gacgatctcc ttcgcctcgg gctcacgctc
     1261 gtacgcctcg cggagctcgc gcgactcctt cagtgcttcc tcgatcgggt gctcgcggcc
     1321 gaggacggcg ggcgggtaca tcttgcagag gcggtccccg atcgacgccg ggtacccgag
     1381 cacccgcgcg gcgtcgcgga tgccctgctt ccccttgatc gtctggaacg tgatgatctg
     1441 cgcgacgtgg tcgtgcccgt agcggtcggc cacgtagcgg atcacctcgt cgcgacgacg
     1501 ctcgtcgaag tccatgtcga tgtcgggcat ctgcttgcgc tcggggttga ggaaacgctc
     1561 gaagatcagc ccgtagcgca gtgggtcgag gtcggtgatg cgcaggcaag acgagaccac
     1621 ggaccccgcc gccgaacctc tccccggc
//
LOCUS       DASZOB010000426         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_119479, whole genome shotgun sequence.
ACCESSION   DASZOB010000426 DASZOB010000000
VERSION     DASZOB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119479"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VG709_06705"
     CDS             complement(<1..959)
                     /locus_tag="VG709_06705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWC32801.1"
                     /translation="MLPFVVIFLVAAVVAYAATPAVRWLATRFGAIDRPSDRKVHPKP
                     TPTLGGIAIWFAVAVSLGVAYLMPPFRDLFEISSEPLGALLAGTIVVVLGAYDDVRGL
                     SVPAKVAGQVLAAGVLILMGVQLLYFWFPGLGVLSLSADLAVPLTLIWVVAMINAVNL
                     VDGLDGLAVGVVAIAAAAFFWYAFETGEGTRTLYAGPSPATLLSAIVAGAAVGFLPHN
                     FHPARIFMGDSGSMLLGLLLAAATISGVGRTDAPTNSDLAALAIPVAIPLLVLAVPLT
                     DVMWAIIRRLRRKRPVFRPDKEHIHHRLLEIGHTHRQAVLLMYL"
     gene            complement(960..2216)
                     /gene="glyA"
                     /locus_tag="VG709_06710"
     CDS             complement(960..2216)
                     /gene="glyA"
                     /locus_tag="VG709_06710"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWC32802.1"
                     /translation="MKEWVADWDALKATDPEVAQAMVDELERERSTIRLIASENYTSP
                     AVLAALASTMNNKYAEGYPGRRYYGGCRFVDVSETLAITRAKQLFGAEHANVQPHAGA
                     QANMAAYFAFMEPGDTMLGMSLPHGGHLTHGSKVNFSGKWFDVVGYEVDRETELIDMD
                     RVRDLAREHRPKVIVAGATAYPRTIDFPAFREVADEVGATFLVDAAHFIGLVAGGAYP
                     NPVPHADVVTFTAHKTLRGPRGAMILCREEHAKAIDKAVFPMMQGGPLENSIAAKAVA
                     LKEAMSPEFSDYAHRVVRTARALASGLQEEGMRLVSGGTDSHLALVDLRPAGVTGQDA
                     EAACDDVGITLNKNAIPFDPLPPATASGIRVGTPGPATLGMDEPEMKEIAGVIGEVIR
                     GGGDEDVRRRARERVTELAQRFPAYG"
     gene            complement(2213..2665)
                     /locus_tag="VG709_06715"
     CDS             complement(2213..2665)
                     /locus_tag="VG709_06715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HWC32803.1"
                     /translation="MRIAIGSDHAGYRLKEDLKQLLKEDGHDVTDVGTDSEEPTDYPR
                     FCAAAARLVRDGEADRGVVLGGSGQGEQMSANKVRGVRAALCNDLYTARLSREHNDAN
                     VLSIGARVVAPALAREIVRTWLSSDFEGGRHVRRLEQIAEIEREEASG"
     gene            complement(2667..3263)
                     /locus_tag="VG709_06720"
     CDS             complement(2667..3263)
                     /locus_tag="VG709_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HWC32804.1"
                     /translation="MLSVLIVCTGNICRSPMAEGFLVERSERLFGGSVRVRSAGTWAR
                     RAGPPMSESIQAAAERGVDISALSSSPVEPEMIRDSDLVLTMTAEQRDEVLALAPEAR
                     DRTFTLKEAVALFAAMPTAAGGERDALLARIEEAARLRETGSVVVSDEDVSDPLGMSI
                     DTYRAVAWEIENLVDALVAGLSGSERREETAEAKKVWG"
     gene            complement(3297..4169)
                     /locus_tag="VG709_06725"
     CDS             complement(3297..4169)
                     /locus_tag="VG709_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWC32805.1"
                     /translation="MTARRPTVVVLLILAVACPSAPPEPRGPVTARERVRGPLPFAVI
                     GDFGTGEEAQHVVADALRRVARERRIPVLVTTGDNVYEDGNPDDFDDAWHEPYGWVER
                     RGIDVVASLGNHDLGTDDGVPVMELLGMPNPWYARRYGDVEFFVLDSGALGAPAQLRF
                     VADAMRRSKAPWKIAVVHHAPYGCSKHGGNRVVRELAVPVLREAGADVVLAGHEHNYQ
                     RFPPFGGMTVVVSGGGGAELHDVGECPDGTPDPVAVIDDAHHFLIGVANERRLRIEAV
                     TADGSVADRVVIRA"
     gene            complement(4166..>4411)
                     /locus_tag="VG709_06730"
     CDS             complement(4166..>4411)
                     /locus_tag="VG709_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HWC32806.1"
                     /translation="ARGLRERTGPRAVTSANRSGEPTPLDCGGVVDALGDGVSVYLCA
                     GRLPGGVASTVVDLTSEEPQILREGAIASDEVLGALT"
BASE COUNT          633 a         1613 c         1559 g          606 t
ORIGIN      
        1 agatacatca ggaggacggc ctggcggtgc gtgtgcccga tctcgagcag gcgatggtgg
       61 atgtgctcct tatcgggccg gaacaccggg cgcttccggc gaagccgcct gatgatcgcc
      121 cacatgacgt cggtgagcgg gaccgccagc acgagcagcg ggatcgcgac cgggatcgcg
      181 agtgcggcga ggtcgctgtt cgtcggcgcg tccgtgcgcc ccacgcccga gatcgtcgcg
      241 gccgccaaca ggagcccgag cagcatgctc ccggagtcgc ccatgaagat ccgcgccggg
      301 tggaagttgt gtgggaggaa gccgacggcc gcgcccgcga cgatcgcgga taggagcgtc
      361 gccggcgacg gccctgcgta caacgtcctc gttccctcgc cggtctcgaa ggcgtaccag
      421 aagaacgccg ctgccgcgat cgccacgacc ccgacggcga gcccgtcgag cccgtcgacg
      481 aggttcaccg cgttgatcat cgcgacgacc cagatgagcg tgagcggtac cgcgaggtcc
      541 gcggagagcg acaggacgcc cagtccgggg aaccagaagt agaggagctg cacccccatg
      601 aggatcagca cgcccgcggc gaggacctga cccgcgacct tcgccggcac cgagaggccg
      661 cgcacgtcgt cgtaggcgcc gagcaccacc acgatcgtcc ccgcgagcag cgcgccgagc
      721 ggctcggacg agatctcgaa gaggtcgcgg aacggcggca tgagatacgc gaccccgagc
      781 gagacggcga cagcgaacca gatcgcgatg ccgccgagcg tcggcgtggg cttcggatgg
      841 accttccggt ccgatggccg atcgatcgcg ccgaaccgtg tcgcgagcca ccggaccgcc
      901 ggcgtcgcgg catacgccac gacggcggcg accaggaaga tcacgacgaa ggggagcacc
      961 tagccgtacg cggggaagcg ctgggccagc tcggtcacgc gctcgcgcgc gcggcgcctc
     1021 acgtcctcgt cgccgccgcc ccggatgacc tcgccgatca cgccggcgat ctccttcatc
     1081 tcgggctcgt ccatcccgag cgtcgccggg cccggcgtgc cgacgcggat ccccgatgcg
     1141 gtcgccggcg gcaaggggtc gaacgggatc gcgttcttgt tgagggtgat cccgacgtcg
     1201 tcgcacgcgg cctccgcgtc ctgccccgtg acgccggccg gccggaggtc gaccagcgcg
     1261 aggtgggagt cggtcccgcc ggagacgagc cgcatcccct cctcctggag cccggacgcg
     1321 agcgcccgcg cggtgcgaac gacgcggtgc gcgtagtcgg agaactccgg cgacatcgcc
     1381 tccttgagcg ccacggcctt cgccgcgatg gagttctcga gggggccgcc ctgcatcatc
     1441 gggaagacgg ccttgtcgat ggccttcgcg tgttcctcgc ggcagaggat catcgcgccg
     1501 cgcggcccgc gcagcgtctt gtgggccgtg aacgtcacga cgtcggcgtg cggcaccgga
     1561 ttcggatacg cgccgccggc cacgagcccg atgaagtgcg cggcgtcgac gaggaaggtc
     1621 gcgccgacct cgtcggcgac ctcccggaac gccgggaagt cgatcgtgcg cgggtacgcg
     1681 gtcgcgccgg cgacgatgac cttcggccgg tgctcgcgcg cgaggtccct gacgcggtcc
     1741 atgtcgatga gctcggtctc gcggtcgacc tcgtacccga cgacgtcgaa ccacttcccg
     1801 gagaagttga ccttcgagcc gtgagtgagg tgcccgccgt gcgggaggct catcccgagc
     1861 atggtgtcgc cgggctccat gaacgcgaag taggcggcca tgttcgcctg cgcgccggcg
     1921 tgcggctgga cgttcgcgtg ctcggcgccg aagagctgct tcgcgcgggt gatcgcgagc
     1981 gtctcgctga cgtcgacgaa gcggcacccg ccgtagtagc ggcggcccgg gtacccctcg
     2041 gcgtacttgt tgttcatcgt cgaggcgagc gccgcgagca ccgcggggct cgtgtagttc
     2101 tccgaggcga tcagccggat cgtgctccgc tcccgctcga gctcgtcgac catcgcctgc
     2161 gccacctcgg gatccgtcgc cttcagcgca tcccagtcgg cgacccactc cttcatccgc
     2221 tcgcctcctc gcgctcgatc tccgcgatct gctccagcct gcgaacgtga cggccgccct
     2281 cgaagtcgct cgagagccac gtccgcacga tctcgcgcgc gagcgcgggc gcgaccacgc
     2341 gcgcgccgat cgagagcacg ttcgcgtcgt tgtgctcgcg cgacagcctc gccgtgtaga
     2401 ggtcgttgca gagcgccgcg cggacgccgc gcaccttgtt ggccgacatc tgctccccct
     2461 gtcccgaccc gccgagcacg acgcctctgt cggcctcccc gtcgcggacg agccgcgcgg
     2521 ccgcggcgca gaacctcggg tagtcggtgg gctcctcgga gtccgtcccc acgtcggtca
     2581 cgtcgtgacc gtcctccttg aggagctgct tcaggtcctc cttgagccgg taccccgcat
     2641 ggtccgaccc gatcgcgatc cgcacgctat ccccacacct tctttgcctc tgcggtctcc
     2701 tcgcgccgct ccgaccccga gagcccggcg acgagggcgt ccacgaggtt ctcgatctcc
     2761 cacgcgacgg cgcggtaggt gtcgatcgac atgccgagcg ggtcggagac gtcctcgtcg
     2821 gagacgacga ccgaccccgt ctcgcggagg cgcgcggcct cctcgatgcg cgccaggagg
     2881 gcgtcgcgct cgcccccggc ggccgtcggc atcgccgcga agagcgccac ggcctccttc
     2941 agcgtgaagg tccggtcgcg cgcctctggc gcgagcgcga gaacctcgtc gcgctgctcg
     3001 gccgtcatcg tgaggacgag gtctgagtcg cggatcatct cgggctcgac cggactcgac
     3061 gacaacgccg agatgtcgac gccgcgctcc gcggcggcct ggatcgactc cgacatcggc
     3121 gggccggcgc ggcgggccca cgtgccggcc gaccggacac gaacactccc cccgaagaga
     3181 cgctccgagc gttccacgag gaagccctcg gccatcgggg atcgacagat gttgcccgtg
     3241 cacacgatga ggacggacag cacggcgcga gtctagcatc ggcccttccg gcggcttcag
     3301 gcgcggatca ccacgcggtc ggctacggag ccgtcggccg tcacagcctc gatccggagc
     3361 cggcgctcgt tcgccacccc gatcaggaag tggtgcgcgt cgtcgatcac cgcgacggga
     3421 tccggcgtgc cgtcgggaca ctcgccgacg tcgtggagct cggcgccgcc gcctcccgag
     3481 acgacgaccg tcatgccgcc gaacggcggg aaccgctggt agttgtgctc gtgacccgcg
     3541 aggacgacgt cggcgccggc ctcgcggagc accggcaccg cgagctcgcg gacgacgcgg
     3601 ttgcccccgt gcttcgagca gccgtacggc gcgtggtgga cgaccgcgat cttccacgga
     3661 gccttcgagc gccgcatcgc gtctgcgacg aaccgcagct gcgcgggggc cccgagcgcg
     3721 ccgctgtcga ggacgaagaa ctcgacgtcg ccgtagcgcc gggcgtacca ggggttcggc
     3781 atcccgagca gctccatcac cggcacgccg tcgtcggtcc cgagatcgtg gttcccgagc
     3841 gacgcgacga cgtcgatgcc acggcgctcg acccagccgt acggctcgtg ccaggcgtcg
     3901 tcgaagtcgt ctgggttgcc gtcctcgtag acgttgtcgc cggtcgtcac gaggaccggg
     3961 atccggcgct cgcgggcgac gcggcggagc gcgtcggcta ccacgtgctg agcctcctcg
     4021 ccggtgccga agtcgcctat gacggcgaac gggagcggcc cgcggacccg ctcgcgcgcc
     4081 gtgaccgggc cgcgcggctc gggcggcgcc gatggacagg cgaccgcgag gatgaggagc
     4141 acgacgaccg tggggcggcg cgcggtcacg tcagcgcgcc gaggacctcg tccgacgcga
     4201 tcgcgccctc gcggaggatc tgcggctcct ccgacgtgag gtcgacgacg gtcgacgcca
     4261 cgccgcccgg cagccgcccg gcgcagaggt agacggacac cccgtcgccg agcgcatcga
     4321 cgacgccgcc gcagtcgagc ggcgtcggct ctccagagcg gttcgcgctc gtgaccgcgc
     4381 gcgggccggt ccgctcgcgc agtccccgcg c
//
LOCUS       DASZOB010000427         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29701, whole genome shotgun sequence.
ACCESSION   DASZOB010000427 DASZOB010000000
VERSION     DASZOB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29701"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VG709_06735"
     CDS             complement(<1..1012)
                     /locus_tag="VG709_06735"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWC32807.1"
                     /translation="MTAHSDLSSRSAELPLYEPHGPREECGVIGIYAPGESVARLAYF
                     GLHALQHRGQESAGIASSDRGELRLHKRLGLVGQIFDEESLSGLDGQIAIGHTRYSTT
                     GSNTLPNVQPVVARGDMGEVMLGHNGNLVNAVELRQELQRTGIQFATGSDTEVLARLI
                     AGGEGTTWADRVAGAFRRARGAFSFTMLTADSLVAARDPIGFRPLVLGRFPGVRGGWA
                     VASESTALDAMGAEFVRDVEAGEILTIGPQGVESVRVASPAGTEKLCVFEYVYLARPD
                     SVMRGRLLYEARLRMGRRRAVEHPADADLVISVPDSAVPAAIGYAEESGLPYREGLIK
                     SRY"
     gene            1184..>1652
                     /locus_tag="VG709_06740"
     CDS             1184..>1652
                     /locus_tag="VG709_06740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWC32808.1"
                     /translation="MLSFYRTIGLLTPIFAIACAVALLVTLWSADGEEQLTLIVLTAL
                     GALAGGTLVAFFGWVLITWRLDRFSRALERTITSRDPTKLRVRGVPAERRLARAFNAA
                     ARALAQVEAKSKIDALTGVANREVVLTRRAQEVERANRYLEPLSAAFIDIDRFK"
BASE COUNT          241 a          579 c          588 g          244 t
ORIGIN      
        1 cgtatcggct cttgatcagg ccctcgcgat acgggagacc gctctcctcg gcgtagccga
       61 tcgcggcggg cacggccgag tccggcacgg agatcacgag gtcggcgtcc gccggatgct
      121 cgaccgcccg ccgccggccc atgcggagcc gcgcctcgta gaggaggcgg ccgcgcatga
      181 cgctgtcggg acgcgccagg tacacgtact cgaacacgca gagcttctcc gtccccgccg
      241 gcgaagcgac gcggaccgac tccacgccct ggggtccgat ggtgaggatc tcccccgcct
      301 cgacgtcacg gacgaactcc gcgcccatgg cgtcgagtgc cgtgctctcg ctcgccacgg
      361 cccagccacc gcggacgccg gggaagcggc ccaggaccag cggtcggaag ccgatggggt
      421 cgcgcgccgc gacgagcgaa tcggcggtga gcatcgtgaa cgagaaggcg ccgcgcgccc
      481 gacggaaggc accggcgacc cgatccgccc acgtcgtccc ctcgcccccg gcgatgagcc
      541 tcgcgagcac ctccgtgtcg ctgcccgtgg cgaactggat ccccgtgcgc tggagctcct
      601 ggcggagctc gacggcgttc acgagattcc cgttgtgccc gagcatgacc tcgcccatgt
      661 cgccgcgcgc gaccaccggc tggacgttcg gcaaggtgtt cgagccggtg gtgctgtagc
      721 gcgtgtggcc gatggcgatc tgaccatcga gcccggacag tgactcctcg tcgaagatct
      781 gcccgacgag gccgaggcgc ttgtggaggc gcagctcgcc gcggtccgag gaggcgatcc
      841 ccgccgactc ctgcccgcga tgctggagcg cgtgaagtcc gaagtaggcg aggcgcgcga
      901 ccgattcgcc gggtgcgtag atgccgatca cgccgcactc ttcccgaggg ccgtgcggtt
      961 cgtagagggg cagctcggcg ctccgcgacg acaggtcgga gtgggccgtc acgcggctgg
     1021 gagggtcgat ccagtcggca cgtcgcgatg ataccggagc gggaccgccg cccgcgagca
     1081 tgtgacgatc gggccatacc ctcagggacg agcgacgtcc atcctcgcgt cccgagtgag
     1141 tctcgccatg gagcgggcgg gtccggggtc gtgagagagg gttgtgctca gcttttaccg
     1201 gacgatcggt cttctgacgc cgatcttcgc gattgcctgc gcggtcgcgc tcctcgtgac
     1261 gctgtggtcc gcagacggcg aggagcagct caccctgatc gtgctcaccg cgctcggggc
     1321 gctcgccggc ggaacgctcg tcgcgttctt cgggtgggtg ctgatcacct ggcgtctcga
     1381 ccgcttcagc cgcgccctcg aacgcaccat cacgtcgagg gatccgacga agctccgcgt
     1441 caggggcgtg cccgccgaac gacggcttgc gcgcgccttc aatgccgcgg cgcgggcgct
     1501 cgcccaggtc gaggccaagt cgaagatcga cgcgctcacc ggggtcgcga atcgagaggt
     1561 ggtgctcacc cggcgggcgc aggaggtcga gcgtgcgaat cgctacctcg agccgctgtc
     1621 ggcggccttc atcgacatcg accgcttcaa gc
//
LOCUS       DASZOB010000428         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_150381, whole genome shotgun sequence.
ACCESSION   DASZOB010000428 DASZOB010000000
VERSION     DASZOB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150381"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1550
                     /locus_tag="VG709_06745"
     CDS             222..1550
                     /locus_tag="VG709_06745"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWC32809.1"
                     /translation="MTLSNEAEFVLRTVEERGIRFIRLWFTDVLGFLKSFAITPAELE
                     TAFTEGMGFDGSSIEGFSRIQESDMVAWPDPSTFQIIPWQSEHQVARMFCDIRNPDGT
                     AFEGDPRYVLKHQLGRAADLGFRFYVGPELEYFYFKDERGTEFLDRGGYFDLTPLDVA
                     TGYRKRTVQYLEAMGIPVEYVHHEVAPSQHEIDLRYADALSMADNVMTYRLTVKEVAH
                     EAGVYATFMPKPVHGENGSGMHTHQSLFEGERNAFFDATDEHHLSKTAKGYIAGILRH
                     AREITLVLNQWVNSYKRLVPGYEAPVYVCWARRNRSALVRVPMYKPGNEGAARIELRS
                     PDPSCNPYLAFAAMLAAGLRGIEEEYELAPEASNNIYEMAEDERRAAGIESLPEDLYE
                     AIRIAEGSELLHEVLGAHVFDYLVRNKREEWDAYKAYVTPFELDRYLPLL"
     gene            complement(1561..2019)
                     /locus_tag="VG709_06750"
     CDS             complement(1561..2019)
                     /locus_tag="VG709_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWC32810.1"
                     /translation="MEPQPGPATTPTMMPVPEGSAALDEALRRVGDRWSLLVVQALLE
                     GPLRWGELIDAVPGIASNVLTERLRRLERERVVSAAPYSERPRRYSYELTPAGRELAG
                     ALRLLADWGASGGGPEAPRHRPCGTPMEARWFCPTCAMPVEDDDDDLEIV"
BASE COUNT          360 a          758 c          762 g          322 t
ORIGIN      
        1 accacagccg gacgaggcgc acgcggcgct cctggacctc gcggacgacc gctgccgcgt
       61 ccatcgagcg gatggtagcc cgtgcggagc gtggtccagc ggagcgtcgc gggtcggagc
      121 gaagccccga gacgaaccgc cgacgggcgc cctccggggc gcccctcgac tttttacgcg
      181 cccgtaaccg tgacgctgcg gggcttcggt accctcgccg catgaccctc tccaacgaag
      241 cggagttcgt gctgcgcacc gtcgaggaac gcggcatccg cttcatccga ctgtggttca
      301 ccgacgtgct cggcttcctc aagtcgttcg cgatcacgcc ggcggagctc gagaccgcgt
      361 tcacggaagg catggggttc gacggctcct cgatcgaggg gttctcgcgc atccaggaat
      421 cggacatggt ggcctggccg gacccctcga cgttccagat catcccgtgg cagtcggagc
      481 accaggtggc gcgcatgttc tgcgacatcc gtaacccgga cggcaccgcg ttcgagggcg
      541 acccgcggta cgtgctgaag catcagctgg gtcgcgcggc cgacctcggc ttccgcttct
      601 acgtcggacc cgagctcgag tacttctact tcaaggacga acgcgggacc gagttcctcg
      661 accgcggggg gtacttcgac ctcacgccgc tcgacgtcgc gaccggctac cgcaagcgca
      721 ccgtgcaata cctagaggcg atggggatcc ccgtcgagta cgtgcaccac gaggtcgcgc
      781 cgtcgcagca cgagatcgac ctccgctacg ccgacgcgct ctcgatggcg gacaacgtga
      841 tgacgtaccg cctcacggtc aaggaggtag cgcacgaggc cggcgtctac gccacgttca
      901 tgccgaagcc ggtccacggc gagaacggca gcggcatgca cacgcaccag tcgctgttcg
      961 agggcgagcg caacgcgttc ttcgacgcga ccgacgagca tcacctgtcg aagaccgcga
     1021 aggggtacat cgccgggatc cttcgccacg cgcgcgagat cacgctggtg ctgaaccagt
     1081 gggtgaactc ctacaaacgg ctcgtgcccg gctacgaggc gccggtctac gtgtgctggg
     1141 cccggcggaa ccgctcggcg ctggtccgcg tgccgatgta caagcccggg aacgagggcg
     1201 cagcacggat cgagctccgc tcccccgacc cctcgtgcaa cccctacctc gcgttcgccg
     1261 cgatgctcgc ggccggtctc cgcggcatcg aggaggaata cgagctcgcc ccggaggcct
     1321 ccaacaacat ctacgagatg gcggaggacg agcgacgagc ggccgggatc gagtcgctcc
     1381 cggaggacct ctacgaggcg atccgcatcg ccgagggctc ggagctcctg cacgaggtcc
     1441 tcggggcgca cgtcttcgac tacctcgtgc ggaacaagcg cgaggagtgg gacgcctaca
     1501 aggcgtacgt cacgccgttc gagctggacc ggtatctgcc gctgctgtaa ggaccagcgt
     1561 ctagacgatc tcgaggtcgt cgtcgtcgtc ctcgacgggc atcgcgcagg tcgggcagaa
     1621 ccacctcgcc tccatcggcg ttccgcacgg gcggtggcga ggcgcctccg gcccgccgcc
     1681 cgacgccccc cagtcggcga gaagccgcag ggcgccggcg agctcccggc ccgccggtgt
     1741 cagctcgtag gagtaccgac gcgggcgctc ggagtacggc gccgccgaga ccactcgctc
     1801 gcgctcgagc ctgcgcagtc gctccgtgag gacgttcgag gcgatgcctg ggacagcgtc
     1861 gatcagctcg ccccaccgga gcggcccctc gaggagggcc tggacgacca gcaggctcca
     1921 ccggtcgcca acccggcgga gcgcctcgtc cagcgccgcg gatccttcgg gcacgggcat
     1981 catcgtaggt gtcgtggcgg gtccgggttg cggttccacg tcgcggattg taacttgcaa
     2041 agagcaagtt gctctcggaa aggagctgag atgggagtga tcgaggagat cgcagaggcg
     2101 gcgcagaccg ccctggcgag ggcgggacgg tcggtggtcg gggtcgggcg cacggggccc
     2161 cgtgggtcag gggtcgtcat cggcgacggc gaggtcctca cc
//
LOCUS       DASZOB010000429         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_79918, whole genome shotgun sequence.
ACCESSION   DASZOB010000429 DASZOB010000000
VERSION     DASZOB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79918"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VG709_06755"
     CDS             <1..779
                     /locus_tag="VG709_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32811.1"
                     /translation="RTLVAQALFAAIAERGSDAVGYAYRRGDGPIVVHKQRTGASDLL
                     EGIAIPEDARQALLHVRDYTKGHPSLAANNHPIRHGAVVGIHNGIVANDEELFERHGF
                     RRAEPGMTVDSEAIFALVEEAEDATEALAEIHGSMATAWVDERRPERVFTGRGMGRPL
                     WLGEGEEEVLFASTRDALELTERYADVTLRKREVPEGRLLTLAHGRVAGTASFAPDRS
                     FTEEALPAVRAPGERRSCLERLAALAQRRHAPVGVRPAAR"
     gene            complement(826..>1971)
                     /locus_tag="VG709_06760"
     CDS             complement(826..>1971)
                     /locus_tag="VG709_06760"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HWC32812.1"
                     /translation="AMFGPAAALPLALTVAAVHWSLVRHPVQQAFFNAGVFTLASLAA
                     ATVFSLGWEHDDLGRLLSALAGLAAGGAYFLVKTGLFSGVMALEGHDRWWRVWNERFS
                     WLLPRYAAFGLIGGAIALAYEALGLYGLAVFALPLLLMLKTMAAYIGHMRLSSEKLGK
                     AADTIRVQNASLEQANRLLRERSTAAMESLSATVDARDAYTAGHSRRVLDLALALGRE
                     LGLSRQELDLLGRAALFHDIGKLAVPDAILLKPGPLDADEWAVMQRHAEEGARIIDRL
                     GFLDDAVPAIRHHHERFDGSGCPDGVRGTEIPLGARIIHLADAVDSMMTTRIYRAARS
                     VPAVVDEVRAGTGTQFCPSCVAALDRLIDKDGLVHLGIRIPALATAS"
BASE COUNT          295 a          693 c          731 g          252 t
ORIGIN      
        1 accggaccct cgtcgcgcag gcgctcttcg cggcgatcgc ggagcggggc tcggatgccg
       61 tcgggtacgc gtaccggcgt ggcgacgggc ccatcgtcgt ccacaagcag cggacgggcg
      121 cgagcgactt gctggaaggg atcgccatcc ccgaggacgc gcgccaggcc ctccttcacg
      181 tccgcgacta cacgaagggc catccttcgc tcgccgcgaa caaccatcct atccgtcacg
      241 gcgccgtcgt cgggatccac aacggcatcg tggccaacga cgaggagctc ttcgagcgcc
      301 acggcttccg gcgcgccgag ccgggcatga cggtcgattc cgaggcgatc ttcgccctcg
      361 tcgaggaggc ggaggacgcc accgaggcgc tcgccgagat ccacggctcg atggcgaccg
      421 cctgggtcga cgagcgccgt cccgagcgcg tcttcaccgg gcgtggcatg ggccgcccgc
      481 tctggctcgg cgagggcgag gaagaggtcc tgttcgcctc cacgcgcgac gcgctcgagc
      541 ttaccgagcg ctacgccgac gtcacgctca ggaagcgcga agtgccggag ggccgcttgc
      601 ttacgctcgc ccacggccgc gtcgccggca cggcgtcgtt cgcgcccgac cgttcgttca
      661 ccgaagaggc gctcccggcc gtacgtgccc cgggagagcg gcgctcgtgc ctcgagcggc
      721 tggccgctct cgcccaacgc cgccacgcgc ccgtcggcgt ccgcccagca gctcggtagt
      781 aggagggctt gccccgcacg ctccccgctt cgcgcgcatc gaccgttacg acgcggtggc
      841 gagcgccgga atgcggatgc cgaggtgcac gaggccgtcc ttgtcgatca ggcggtcgag
      901 cgccgccacg cagctcgggc agaactgcgt gccggtgccg gcacgaacct cgtcgacgac
      961 cgcgggcacc gaccgggcgg cgcggtagat ccgcgtcgtc atcatcgagt cgacggcgtc
     1021 ggccaggtgg atgatccgcg cgccgagcgg gatctcggtg ccgcgcacgc cgtccgggca
     1081 gccggagccg tcgaaccgct cgtggtggtg acggatggcg ggaacggcgt cgtcgaggaa
     1141 cccgaggcgg tcgatgatcc gcgcgccctc ctccgcgtgc cgttgcatca cggcccactc
     1201 gtcggcgtcg agcggtccag gcttcaacag gatggcgtcc gggacggcga gcttgccgat
     1261 gtcgtggaag agcgccgccc gcccgagcag gtcgagctct tggcgggaga ggccgagctc
     1321 gcggccgagc gcgagggcga ggtcgagcac gcgccgggaa tggccggcgg tgtaggcgtc
     1381 gcgcgcgtcg acggtcgccg agagactctc catagccgcg gtcgaccgct ccctgaggag
     1441 ccggttcgcc tgctcgagcg acgcgttctg gacgcggatc gtgtcggccg ccttgccgag
     1501 cttctcggac gagagccgca tgtggccgat gtacgcggcc atcgtcttca gcatgagcag
     1561 gagcgggagc gcgaagaccg cgaggccgta gaggccgagg gcctcgtacg cgagcgcgat
     1621 cgcacctccg atcaggccga aggcggcgta gcggggtagg agccacgaga accgctcgtt
     1681 ccagaccctc caccagcgat cgtgcccctc gagcgccatc acgcccgaga agagccccgt
     1741 cttgaccagg aagtaggcgc cgccggcggc gaggccggcc agcgcggaca ggagccggcc
     1801 gaggtcgtcg tgctcccacc cgagggagaa gacggtcgcg gcggcgagcg aggcgagcgt
     1861 gaagaccccg gcgttgaaga acgcctgctg aaccgggtgg cgcacgaggc tccagtgcac
     1921 cgcggcgacg gtgagcgcga gcggaagcgc ggcggcgggg ccgaacatcg c
//
LOCUS       DASZOB010000430         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_29789, whole genome shotgun sequence.
ACCESSION   DASZOB010000430 DASZOB010000000
VERSION     DASZOB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29789"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VG709_06765"
     CDS             complement(<1..183)
                     /locus_tag="VG709_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32813.1"
                     /translation="MGDPRDAARRALRDAAGDARSGAAEIARRAAEALAALPRDDVPG
                     AVATLVRAHPSMAPLWR"
     gene            202..903
                     /locus_tag="VG709_06770"
     CDS             202..903
                     /locus_tag="VG709_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32814.1"
                     /translation="MDEDAIREALERVGAAAEPTLEVVHPGRAPAASRLGMLPGSFDP
                     MTVAHAALAGALNRDGCDLVLLVYSARTLPKQDHGGRSAAPPLLAPEARVASLVAFAR
                     RRPWAAGAVSSHGLLVDQVEAAARAFGRPEVVLGIGSDKLLQLLDRAWYEDRDTALKR
                     LFGVARVAYALREGDAAAVERAFGAEPRWRERVTRLALRPDVADISSRELRRRSRRDV
                     EDMVPPEVRPFLGGA"
     gene            complement(913..1119)
                     /locus_tag="VG709_06775"
     CDS             complement(913..1119)
                     /locus_tag="VG709_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32815.1"
                     /translation="MPEDARSELADLFGQAEAEVRGRLKRQPRVHPQFRVGVQVAMRW
                     MREAMAARLGADVLESRSEDDPRP"
     gene            complement(1161..1556)
                     /locus_tag="VG709_06780"
     CDS             complement(1161..1556)
                     /locus_tag="VG709_06780"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWC32816.1"
                     /translation="MDKPTVLFVCVHNAGRSQMAAALLEELCDGRIDVRSAGTQPADE
                     VHEGVVAAMRERGIDLASRRPKRLDERAVREADVVVTMGCGDACPVYPGKRYEDWALP
                     DPAGKPLGDVRAIRDGIEERVRKLIESLG"
     gene            1640..>1938
                     /locus_tag="VG709_06785"
     CDS             1640..>1938
                     /locus_tag="VG709_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWC32817.1"
                     /translation="MAKRRFRLIPREESFYDLFVKQAEVLVEGAKQLQDIVSNFDDVA
                     TKARRLHDIEHEADEVTHEVMRRLNTTFVTPLDREAIHELASSMDDVLDHVDAAA"
BASE COUNT          268 a          713 c          675 g          282 t
ORIGIN      
        1 ccgccagagg ggcgccatcg aggggtgcgc gcggacgagc gtcgcaaccg cgccgggcac
       61 gtcgtcccgc gggagtgcgg cgagagcttc cgcggctcgt ctcgcgatct ccgcggcgcc
      121 cgaccgagcg tcgccggcgg catcccgcag cgcccggcgc gctgcgtcgc gcgggtcgcc
      181 cacgcaaggc atcgtatcgg cgtggacgaa gatgcgatcc gggaggcgct cgagcgggtc
      241 ggcgcggcgg ccgagccgac gctcgaggtc gtgcacccgg ggcgcgcgcc ggcagcgtcg
      301 cgtcttggga tgctcccggg ctcgttcgac ccgatgacgg tcgcccacgc cgcgctcgcc
      361 ggcgcgctca accgggatgg gtgcgacctc gtgctgctcg tctactcggc gcgcactctc
      421 ccgaagcagg accacggcgg ccgctcagcg gcaccgccgc tgctcgcgcc ggaggcccgc
      481 gtcgcgtccc tcgtcgcgtt cgcgcggcgc cgcccgtggg cggcgggcgc ggtctcctcg
      541 cacgggcttc tggtcgacca ggtagaggcg gccgcgcgcg cgttcgggcg gccggaggtc
      601 gtcctcggca tcggatcgga caagctgctt cagctcctcg accgtgcctg gtacgaggat
      661 cgggacacgg cgctcaagcg gcttttcggc gtcgcgcgcg tcgcgtacgc gctccgggag
      721 ggcgatgccg cggccgtcga gcgcgcgttc ggggccgagc cacggtggcg cgagcgcgtc
      781 acgcggcttg ccctgcgccc ggacgtcgcc gatatctctt cccgcgagct gcgtcgacgc
      841 tcccggcgtg acgtcgagga catggtcccg cccgaggtgc ggccgttcct cggcggcgcc
      901 tagccgagat gatcaggggc gcgggtcgtc ctccgatctc gactcgagga cgtccgcccc
      961 gagccgtgcc gccatcgcct cgcgcatcca ccgcatcgcg acctggacgc ccacgcggaa
     1021 ctgcggatga acgcgaggtt gacgcttgag gcggccgcgg acctccgcct ccgcctgtcc
     1081 gaagagatcc gcaagctccg accgcgcgtc ttccggcacg ggcccaaaga tatgcttagc
     1141 cgaagatcgc cgccgcgcaa ctatccgagc gactcgatca gcttgcgcac ccgttcctcg
     1201 atgccgtcgc ggatcgcccg gacgtcgccg agcggcttgc cggcgggatc cggcagcgcc
     1261 cagtcctcgt agcgcttccc cggatagacg ggacaggcat cgccgcagcc catcgtcacg
     1321 acgacgtcgg cttcccggac cgctcgttcg tcgaggcgct tgggtcggcg tgatgcgaga
     1381 tcgatcccgc gctctcgcat cgctgcaacg accccctcgt ggacctcatc ggccggttgt
     1441 gtaccggcgc tccgcacgtc gatgcggccg tcgcacagct cctcgagcag cgcggcggcc
     1501 atctgcgacc gaccggcgtt gtgcacgcac acgaaaagga ccgtcggttt gtccatcgcg
     1561 cacagggtac cgccgggatt ggacactccg ccctgcgtgg tgtatcagtc gccgcgtttc
     1621 ccacccgtgg aggaaaggga tggcaaagcg gcgattccgg ctgatccccc gtgaggaatc
     1681 cttctacgac ctcttcgtca agcaggccga ggtgctcgtc gagggcgcca agcagctcca
     1741 ggacatcgtg tccaacttcg acgacgtggc gacgaaggcc cgcaggctcc atgacatcga
     1801 gcacgaggcc gacgaggtca cgcacgaggt catgcgcagg ctcaacacca cgttcgtgac
     1861 gccgctcgat cgcgaggcca tccacgagct cgccagcagc atggacgacg tactcgacca
     1921 cgtggacgcc gccgccga
//
LOCUS       DASZOB010000431         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_9724,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000431 DASZOB010000000
VERSION     DASZOB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9724"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VG709_06790"
     CDS             complement(<1..633)
                     /locus_tag="VG709_06790"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32818.1"
                     /translation="MNGVAVDVCVDVPRLELDRPFTYLLPDGVEEATGHLVSVPFHGR
                     TVKGWVLGPARELPRRVVRIRGVLSAVPLFGERDLELYRWIAARYVVPLATVISRASP
                     PRIASEEKRDHDSVATDVEGAARVYTILPSYDEARPLLEGGYSSARTIAMNPSPDDEL
                     DVCLEAVDACLATRRDALVVVPEAEPLPATARAVLERFGDVAVSFVGGDER"
     gene            complement(965..2152)
                     /gene="metK"
                     /locus_tag="VG709_06795"
     CDS             complement(965..2152)
                     /gene="metK"
                     /locus_tag="VG709_06795"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWC32819.1"
                     /translation="MPRRYLFTSESVTEGHPDKLADQISDAVLDEILKDDPNGRVACE
                     TLVTTGLVTVAGEISTETYVDIPAVVRRTVTDIGYTDAKFGIDGDTCGVITSIQEQSP
                     DIARGVDHAAEEREEGSADDLDTIGAGDQGMMFGYACRETDELMPMPIAMAHAIAKRL
                     ADVRKAGVLPYLRPDGKSQVTIEYEGDKPVRVDTVLISAQHRDEVDRDSLLRPDVKEH
                     VIDPVLVEFGLEHEDKRVLVNPTGRFEIGGPRADTGLTGRKIMVDSYGGMAPHGGGCF
                     SGKDPTKVDRSAAYMARYVAKNIVASGAAERVQLQVAYAIGTAQPVSFVVETFGTSEV
                     DPVRLEEVALELFDFRPAAIIRDLRLARPLYRETACYGHFGRKGFAWEDVDRVDDLRA
                     ALA"
     gene            complement(2194..>2787)
                     /locus_tag="VG709_06800"
     CDS             complement(2194..>2787)
                     /locus_tag="VG709_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HWC32820.1"
                     /translation="ERFPRNRSSGKMGFAVAAEAAGRGADVTLVVGATQLPPPAGVDA
                     VRVETAEEMRREVLARFENADALVMAAAVSDFRPERATPGKLKKESGPPEVRLTPTAD
                     ILGELGPSKGARVVVGFAAETDDLEGEGLRKLKSKNLDLVVVNRVGAEGTGFEAETNE
                     AMIVSSSDEPPPEPSTRTKAALAAEICDRLAALLADR"
BASE COUNT          393 a          987 c          975 g          432 t
ORIGIN      
        1 ccgttcgtcg ccgccgacga agctcacggc gacgtccccg aaccgctcga ggaccgcgcg
       61 cgccgtcgcg gggagcggct ccgcctccgg gaccacgacc agggcgtcgc gccgcgtcgc
      121 gaggcacgcg tcgacggcct ccaggcagac gtcgagttcg tcgtccgggc tcgggttcat
      181 cgcgatcgta cgcgcgctcg agtacccgcc ttcgaggagc ggacgcgcct cgtcgtacga
      241 ggggaggatc gtgtagaccc gcgcggcgcc ctccacgtcc gtcgcgacgc tgtcgtgatc
      301 acgcttctcc tcggaagcga tgcgcggcgg tgaagcacgc gagatgacgg tcgcgagcgg
      361 gacgacgtac cgggcggcga tccagcggta cagctcgagg tcgcgctcgc cgaatagcgg
      421 gaccgccgag agcacgccgc ggatacgcac gactcgcctc ggaagctcgc gcgccggtcc
      481 gagcacccat cccttcactg tccggccgtg gaacggaacc gacacgaggt gtccggtggc
      541 ctcctccacc ccgtccggga gcaggtacgt gaacggccgg tcgagctcga gccgcggcac
      601 gtcgacacaa acgtcgaccg cgacaccgtt catggcagga gagctgcgcg ggtccggcag
      661 acgtggggcg tggctgtccc gattgtgggc gcgcctgtta cgcacgcagg tccctcccgc
      721 tcccgacacg cgatgagtcg tcgcgcacct gaacgtatgc cggatgtcgc gtgtctccgc
      781 gggtccctcc cacgtgcgcg cggcgcgcca cgtccggctg cccggcgcgg cgctcgactc
      841 cggcggggac ccgcggagca acgtgcgagg cgctggacct tctcggaacg acgcttctgc
      901 ttcacgttgc gtgagcgggg ggatccggac gtcgagacag ccgcgcctcc aaggagatgg
      961 atgtctaggc gagggcggcg cggaggtcgt cgacgcgatc gacgtcctcc caggcgaatc
     1021 ccttgcggcc gaagtgcccg tagcacgccg tctcccggta gagcggccgc gccaggcgga
     1081 ggtcgcggat gatcgccgcc ggccggaagt cgaagagctc gagcgcgacc tcctcgagac
     1141 gaacgggatc gacctcggac gtgccgaacg tctccacgac gaacgaaacc ggctgcgcgg
     1201 tgccgatggc gtaggcgact tggagctgga cgcgctccgc tgcgcccgac gcgacgatgt
     1261 tcttcgcgac gtagcgagcc atgtacgcgg cggaccggtc gaccttcgtg gggtccttcc
     1321 cggagaagca cccgccgccg tgcggcgcca tgccgccgta ggagtcgacc atgatcttgc
     1381 ggccggtgag ccccgtgtcg gcgcgcggac cgccgatctc gaacctgccg gtcggattca
     1441 ccaggacgcg cttgtcctcg tgctcgagac cgaactcgac gagcaccggg tcgatgacat
     1501 gctccttgac gtcgggacga agcaatgagt cgcggtcgac ctcgtcgcgg tgctgcgcgg
     1561 agatcaggac agtgtcgact cggaccggct tgtccccctc gtactcgatc gtgacctggc
     1621 tcttcccgtc ggggcggagg tacgggagga cgccggcctt ccggacgtcc gcgagcctct
     1681 tcgcgatcgc gtgtgccatc gcgatcggca tcggcatgag ctcgtccgtc tcccggcacg
     1741 cgtagccgaa catcatcccc tggtcccccg cgccgatcgt gtcgaggtcg tcggcggaac
     1801 cctcctcgcg ctcttccgcg gcgtggtcga ccccgcgggc gatgtccggg gactgttcct
     1861 ggatcgacgt gatcacaccg cacgtatcgc cgtcgatccc gaacttcgcg tcggtgtagc
     1921 cgatgtcggt gaccgtgcga cggacgaccg cggggatgtc gacgtacgtc tcggtcgaga
     1981 tctctccggc gaccgtgacg aggccggtcg tgaccagcgt ctcgcatgcg acgcggccgt
     2041 tcgggtcgtc cttgaggatc tcgtccagga ccgcgtcgga gatctgatcc gcgagcttat
     2101 ccgggtgtcc ctcggtcacg gattccgatg tgaacaggta gcggcgcggc atgtcccggg
     2161 gattatacgg gcggtcccgg agccggccgc ggctcagcgg tcggcgagga gggcggcgag
     2221 gcggtcgcag atctcggccg cgagcgcggc cttcgtgcgc gtcgagggct cgggcggcgg
     2281 ctcgtcgctc gacgagacga tcatcgcctc gttcgtctct gcctcgaagc ccgtcccctc
     2341 ggcgcccacg cggttcacga cgacgaggtc gaggttcttc gacttgagct tccggagccc
     2401 ctcgccctcg aggtcgtcgg tctccgcggc gaaaccgacc acgacgcggg cgcctttgct
     2461 cggtccgagc tcccccagga tgtccgcggt cggcgtaagc cggacctcgg gcggaccaga
     2521 ctccttcttg agcttcccgg gggtcgctcg ttcgggacgg aagtccgaga cggccgccgc
     2581 catcaccagc gcgtcggcgt tctcgaagcg cgcgaggacc tcacgccgca tctcctccgc
     2641 ggtctcgacc cgtaccgcat cgacaccggc gggcggcggg agctgcgtcg caccaacgac
     2701 cagcgtgacg tcggcgccgc gcccggcggc ctccgccgcg accgcgaagc ccatcttgcc
     2761 gctcgagcgg ttcctcggga accgctc
//
LOCUS       DASZOB010000432         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_150425, whole genome shotgun sequence.
ACCESSION   DASZOB010000432 DASZOB010000000
VERSION     DASZOB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150425"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VG709_06805"
     CDS             <1..933
                     /locus_tag="VG709_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWC32821.1"
                     /translation="RYSALTVFGLLPAALIGAELDGLLKQAAEAAAACRQPDPAENPG
                     AFLGAVLGEAAIAGRDKATFVGTTQTPFGAWAEQLLAESTGKEGKGVVPVDGEAAGPA
                     EVYGDDRLFLTIGDENRLRPFVSLPDVRRYALGDAFFTFEFATAVAGHVLTIQPFDQP
                     NVQEAKDRTAEALESDEAGVQTLTPLDELLDGIRPGDYVAIQAFVPSRSDLLARLQQV
                     RLRIRDRFRVATTLGLGPRYLHSTGQLHKGGPDTGVFMQVFGDPVEDRAVPGQPYTFG
                     SLIAAQAAGDLAALRDRGRRAGRVRLDELVAWGG"
     gene            949..>1682
                     /locus_tag="VG709_06810"
     CDS             949..>1682
                     /locus_tag="VG709_06810"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWC32822.1"
                     /translation="MRIGMVGLGRMGGNMSVRLLRHEHSPVGYARGVASREAVAQQGV
                     EVVETLDALVAALEPPRAIWLMVPSGAPTESVLGSLRESLADGDLVVDGGNSYFRDSV
                     RHARALGERGIRFVDAGVSGGVWGLENGYCMMVGGEGRDVDTLRPVLDALAPPRGWSH
                     VGPHGAGHFVKMVHNGIEYGLMQAYAEGFEIMRASEFGIDLRDVAGIWRYGSVVRSWL
                     RDLLYAAFEQEGNELERIAGYVQDSG"
BASE COUNT          222 a          571 c          633 g          256 t
ORIGIN      
        1 cgctactccg cgctcacggt cttcgggctg ctgccggcgg ccctcatcgg cgcggagctg
       61 gacggcctgc tgaagcaggc ggcggaagcc gcggcggcgt gccggcagcc cgatcccgcg
      121 gagaatcccg gcgcgttcct cggtgcggtg cttggcgagg cggcgatcgc cgggcgcgac
      181 aaggcgacgt tcgtcgggac gacccagacg cccttcggcg cgtgggcgga gcaactcctc
      241 gccgagtcga ccgggaagga ggggaagggt gtcgtgcccg tcgacggcga ggccgccggt
      301 ccggccgagg tctacggcga cgatcgtctc ttcctcacga tcggagacga gaaccgactc
      361 cggcccttcg tctccctccc ggacgtccgg cgctacgcgc tcggcgacgc gttcttcacg
      421 ttcgagttcg cgacggcggt ggccggtcac gtcctcacca tccagccctt cgatcagccg
      481 aacgtccagg aggcgaagga ccggacggcc gaggccctcg agagcgacga ggcgggggtg
      541 cagacgctca cgccgctgga cgagctcctc gacgggatcc gcccgggaga ctacgtcgcg
      601 atccaggcgt tcgtcccgtc gcggagcgat ctgctcgcac ggctgcaaca ggtccggcta
      661 cggatacgcg accggttccg cgtcgcgacg acgctggggc tgggaccccg ctatctccac
      721 tcgaccggac agctccacaa gggcggtcca gacacgggcg tcttcatgca ggtcttcggg
      781 gaccccgtcg aggatcgggc ggtcccgggc cagccgtaca cgttcggttc gctcatcgcc
      841 gcgcaggccg cgggcgacct cgcggcgctc cgtgatcgag gccgccgagc cggacgggtc
      901 cgtctcgatg aactcgtcgc ctggggcggc tgatctaggg gggctgggat gcggatcggg
      961 atggtcgggc tggggcggat gggtggcaac atgtcggtcc ggctcctccg ccacgagcat
     1021 tcgccggtcg ggtacgcgcg cggcgtcgcg tcgcgcgagg ccgtcgcgca gcagggagtc
     1081 gaggtcgtcg agacgctcga cgcgctcgtc gcggcgctcg agccgccgcg cgcgatctgg
     1141 ttgatggtcc cgtcgggcgc gccgacggag tcggtcctcg gcagcctgcg cgaatcgctc
     1201 gcggacggcg acctcgtcgt cgacggcggc aactcgtact tcagggactc cgtgcgtcac
     1261 gcgcgcgcgc tcggcgagcg tgggatccgg ttcgtggatg cgggcgtctc cggcggcgtc
     1321 tgggggctcg agaacggcta ctgcatgatg gtcggcggcg aggggcgtga tgtcgacacg
     1381 ctccggcccg tcctcgacgc gctcgcgccc ccccgcggat ggtcccacgt cggtccccac
     1441 ggcgccgggc acttcgtgaa gatggtgcac aacggcatcg agtacggcct gatgcaggct
     1501 tacgcagagg ggttcgagat catgcgcgcg tcggagtttg gcatcgacct gcgtgacgtc
     1561 gccggcatct ggcgctacgg atccgtcgtc cggtcgtggc tgcgcgacct gctgtacgcc
     1621 gcgttcgagc aggaggggaa cgagctcgag cggatcgccg ggtacgtcca ggactccggc
     1681 ga
//
LOCUS       DASZOB010000433         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_79951, whole genome shotgun sequence.
ACCESSION   DASZOB010000433 DASZOB010000000
VERSION     DASZOB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79951"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..78
                     /locus_tag="VG709_06815"
     CDS             1..78
                     /locus_tag="VG709_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HWC32823.1"
                     /translation="MQAHAFSATAREKIERAGGRAEVLG"
     gene            104..1399
                     /gene="secY"
                     /locus_tag="VG709_06820"
     CDS             104..1399
                     /gene="secY"
                     /locus_tag="VG709_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWC32824.1"
                     /translation="MLRAFVNAFKIPDLRNKILFTLFVILLYRLGSYIPVPNVDVPLL
                     ANLTGAGQQGFLSVINLFSGGALTRMAVFSLGIMPYITSSIIMQLLTVVIPKLSQWQK
                     QGEVGTKKITQWTRYMTVTLALLQSTGLIFLFNNQFTDPQTGQSVVKAFTPMRVTFMV
                     MTLTAGTALIMWLGELITQRGIGNGMSILIFTSVISTLPAEGASILSGAGGIAFGIVL
                     AIGFAIIMAVVFMEQGQRRIPVQYAKRVVGRRMTSGGSTYIPLKVNQAGVIPIIFASS
                     VLYFPSLLGPVITWEPFQRFVNTTLANPGNPIYMAIYGLLIVFFAYFYTAISFNPTDT
                     ADNIRKYGGFIPGIRPGRPTADHLDHILTRITLPGSLFLATIALIPGIAFTILGVQQQ
                     QGEFLFGGTSILITVGVALETMKQLESQLTMRHYEGFLR"
     gene            1402..2049
                     /locus_tag="VG709_06825"
     CDS             1402..2049
                     /locus_tag="VG709_06825"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWC32825.1"
                     /translation="MRIVLFGPPGAGKGTQAHRLADYYGSRLIATGDIFRMNVRGETP
                     LGLKAKSYMDAGELVPDDVVIQMLTEELAKANNGFILDGFPRTIPQAQALEDALADRD
                     RPLQAVLKFKIPDEVTVARLAGRRTCSRCERTYNIEFKPPRVEGVCDACGGVLIQRED
                     DREETVRHRLEVYHRDTEPLQKFFWDRGLLREVDAVGAVEDITRRAIAVLDDIVE"
     gene            2046..2798
                     /gene="map"
                     /locus_tag="VG709_06830"
     CDS             2046..2798
                     /gene="map"
                     /locus_tag="VG709_06830"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWC32826.1"
                     /translation="MIIRKSPGEVDRLRRAGRITASAIERVLEAVRPGITTAELDAIA
                     ETSIRDAGATPSFKGYRGFPATICASVNEEIVHGIPGRRRLAEGDLLSIDVGAIWDGY
                     HGDSAVSLFVGEPPSGEADKLVRVTEEALEAGIAAIRPGARLSDISHAVEQVAAGAGF
                     SVVREYVGHGIGRSLHEDPPIPNFGPPGRGPELRPGLVVAVEPMVNMGGWETRLLKDG
                     WTVVTADGSLSAHFEHTIAVTGDGHEVLTQRG"
     gene            2791..>3611
                     /locus_tag="VG709_06835"
     CDS             2791..>3611
                     /locus_tag="VG709_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32827.1"
                     /translation="MARRGRARRVLVVAIAASVATSLFAPLPTRAQMKGDPATVFGTG
                     FDRGPVEATIPGLVISDDSAATVRWQTGVARTGTGAYHFVGGNGGADASIHNNRALPL
                     GDLETWSGGAYRFVSFPREEASWLLVTAPTDGRAGANTKPVVSIDGRGRLLLSGGGRT
                     GPVARSAPLDTNRWYYLMLHGRNGAGQRQELFVHDGWDGSLLERVEVTLDVTGDYRNP
                     ITKWGFGTVGDFTGLEYYLDDIFFAPGPANPGTVRVVTTVPRGTVAGGGFGAVGAP"
BASE COUNT          553 a         1269 c         1229 g          560 t
ORIGIN      
        1 gtgcaggccc acgcgttctc cgccacggcc cgcgagaaga tcgagcgggc cggaggccgg
       61 gccgaggtgc tagggtgatc gcccccgcag cggaggccga cgcgtgctga gagcgttcgt
      121 caacgcgttc aagatcccgg atctccggaa caagatcctc ttcacgctct tcgtcatcct
      181 gctctaccgg ctcggctcgt acatcccggt cccgaacgtc gacgtgccgc tgctcgcgaa
      241 tctcaccggc gcggggcagc aggggttcct gtcggtgatc aacctgttct cgggcggggc
      301 gctcacgagg atggcggtgt tctcgctcgg gatcatgccg tacatcacga gctcgatcat
      361 catgcagctc ctcacggtcg tgatcccgaa gctctcgcag tggcagaagc agggcgaggt
      421 cggcaccaag aagatcacgc agtggacccg ttacatgacg gtcacgctcg cgctgctgca
      481 gtcgaccggc ttgatcttcc tgttcaacaa ccagttcacc gacccgcaga ccggccagtc
      541 cgtcgtgaag gcgttcacgc cgatgcgcgt gacgttcatg gtgatgacgc tgacggccgg
      601 gaccgcgctg atcatgtggc tcggcgagct gatcacgcag cgtgggatcg gcaacgggat
      661 gtcgatcctg atcttcacga gcgtgatctc cacgctcccc gccgagggcg cttcgatcct
      721 ctcgggtgcc ggggggatcg cgttcggcat cgtgctcgcg atcggcttcg cgatcatcat
      781 ggccgtcgtc ttcatggagc agggccagcg ccggatcccg gtccagtacg cgaagcgcgt
      841 cgtggggcgc cgcatgacgt ccggcggcag cacctacatc ccgctgaagg tcaaccaggc
      901 cggcgtgatc ccgatcatct tcgcgtcgtc ggtcctctat ttcccgagcc tcctcggtcc
      961 cgtcatcacg tgggagccgt tccagcgctt cgtgaacacg acgctcgcga acccgggcaa
     1021 cccgatctac atggcgatct acgggctcct gatcgtgttc ttcgcgtact tctacacggc
     1081 gatctcgttc aacccgaccg acaccgccga caacatcagg aagtacggtg ggttcatccc
     1141 cggcattcgg ccggggcggc cgaccgccga ccacctcgac cacatcctca cgcgcatcac
     1201 gctgccggga tcgttgttcc tcgcgacgat cgcgctgatc cccggcatcg cgttcacgat
     1261 cctcggcgtt cagcagcagc agggtgagtt cctcttcgga gggacgtcga tcctcatcac
     1321 ggtcggcgtg gcgctcgaga ccatgaagca gctcgagtcg cagctcacga tgcgccacta
     1381 cgaggggttc ctccgctagc catgcgcatc gtcctcttcg gcccgccggg ggccggcaag
     1441 ggaacgcagg cccaccggct cgccgactac tacggctcgc ggctgatcgc caccggcgac
     1501 atcttccgca tgaacgtgcg aggcgagacg ccgctcggcc tgaaggcgaa gtcgtacatg
     1561 gacgccggcg agctcgtccc cgacgacgtc gtgatccaga tgctgaccga ggagctcgcg
     1621 aaggcgaaca acgggttcat cctcgacggc ttcccgagga cgatcccgca ggcgcaggcg
     1681 ctcgaggacg ccctcgccga ccgcgaccgg ccgctccagg cggtgctcaa gttcaagatc
     1741 cccgacgagg tgaccgtcgc ccggctcgcc ggccggcgga cgtgctcgcg ctgcgagcgc
     1801 acctacaaca tcgagttcaa gccgccgcgc gtcgagggcg tctgcgacgc gtgcggcggc
     1861 gtgctgatcc agcgcgagga cgaccgggaa gagacggtcc ggcaccgcct cgaggtctat
     1921 caccgcgaca cggagccgct ccagaagttc ttctgggacc gggggctcct gcgggaggtc
     1981 gacgcggtgg gggcagtcga ggacatcacc cgacgcgcga tcgccgtcct cgacgacatc
     2041 gtcgagtgat catccgaaag tcgcccggcg aggtcgaccg cttgcgccgc gcggggcgga
     2101 tcaccgcctc ggcgatcgag cgcgtcctcg aagccgtccg tccggggatc accacggccg
     2161 agctcgacgc gatcgccgag acatcgatcc gggatgccgg ggcgacgccg tctttcaagg
     2221 gctaccgcgg cttccccgcg acgatctgcg cgtcggtgaa cgaggagatc gtgcacggga
     2281 tcccgggccg gcgtcgcctc gccgagggag atctgctgtc gattgacgtc ggcgcgatct
     2341 gggacgggta ccacggcgac tccgcggtat cgctgttcgt cggcgagccg ccgtccggcg
     2401 aggccgacaa gctcgtccgc gtcacggagg aggccctcga ggccggcatc gccgcgatcc
     2461 ggccgggcgc ccgcctatcg gacatctccc acgccgtcga gcaggtcgcc gccggcgcgg
     2521 ggttcagcgt cgtgcgcgag tacgtcggcc acgggatcgg acgcagcctc cacgaggacc
     2581 cgccgatccc gaacttcggc cctcccggac gaggcccgga gctccgcccg gggctggtcg
     2641 tcgcggtcga gccgatggtc aacatggggg gctgggagac acgactcttg aaggacggct
     2701 ggaccgtggt gaccgccgac ggctcgctct cggcgcactt cgaacacacg atcgcggtca
     2761 ccggtgacgg acacgaggtc ctgacgcagc gtggctaggc gcggccgagc ccgccgcgtg
     2821 ctcgttgtcg cgatcgcggc gagcgtcgcg acgagcctgt tcgctccgct cccgacccgc
     2881 gcgcagatga agggcgaccc ggccacggtg ttcggcaccg gcttcgaccg cggcccggtc
     2941 gaggcgacga tcccggggct cgtgatctcc gacgactccg ccgccaccgt ccgctggcag
     3001 accggcgtcg cgcggaccgg aacgggcgcc taccacttcg tcggcggcaa cggcggcgcc
     3061 gacgcctcga tccacaacaa cagggcgctt cccctcggcg acctcgagac gtggtcgggc
     3121 ggcgcgtacc gcttcgtctc gttcccgcgc gaggaggcgt cgtggctgct cgtcaccgcg
     3181 ccgaccgacg gccgggcggg cgccaacacg aagccggtcg tctcgatcga cgggcgcggg
     3241 cggctgctgc tctcgggggg cggccgcacc ggcccggtcg ctcgatccgc gccgctcgac
     3301 acgaaccgct ggtactacct gatgctccac ggacgcaacg gcgcggggca gaggcaggag
     3361 ctgttcgtcc acgacgggtg ggacggctcg ctcctcgagc gcgtcgaggt cacactcgac
     3421 gtcaccgggg actaccgcaa cccgatcacg aagtgggggt tcggaaccgt cggcgacttc
     3481 acgggcctcg agtactacct cgacgacatc ttcttcgcac cggggcccgc gaacccgggg
     3541 acggtgcggg tcgtgacgac ggtgccgagg ggcacggtcg ccggcggtgg gttcggggcc
     3601 gtcggtgcgc c
//
LOCUS       DASZOB010000434         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_187028, whole genome shotgun sequence.
ACCESSION   DASZOB010000434 DASZOB010000000
VERSION     DASZOB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187028"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VG709_06840"
     CDS             <1..256
                     /locus_tag="VG709_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWC32828.1"
                     /translation="VLVGDREVARLGAGESFGEMALLDQGARSASVVAQESMTLYVID
                     AQDFGRLLEDVPFLTRKILRGVSNRLRSAEQAPAYVWNRF"
     gene            297..887
                     /locus_tag="VG709_06845"
     CDS             297..887
                     /locus_tag="VG709_06845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWC32829.1"
                     /translation="MPDERGARRIVTVPNALSLFRILLIPLFVVLLVGESTRLAGFVV
                     LAAVVSTDWVDGVIARRTGQVTEIGKLLDPIADRLALAAALVTFLVIDVFPLWAALAV
                     LVRDAAVLLAGLWALRRGIRIDVRTIGKYATFTLMWGIPMIAWGNAGMPFDDLMLVLG
                     WVWLPVGLIEYYAASVAYAFDLRKALRARPASRTAS"
     gene            910..>2344
                     /locus_tag="VG709_06850"
     CDS             910..>2344
                     /locus_tag="VG709_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWC32830.1"
                     /translation="MKAVVMAGGQGTRLRPLTSNQPKPMLPIVGRPMMEHILRLLKTH
                     GFDEVVATVQFLASVVRTYFGDGSDLGISLAYATENEPLGTAGSVKNAEHFLDDTFLV
                     ISGDAVTDIDLRAATEFHREREAAVTVVLKRSPNPLDFGIVITDEAGRVDRFLEKPGW
                     GEVFSDTINTGIYVLDPEVLAHIPAGEDFDFAADLFPLLLEKGMPLYGYIAEGFWTDV
                     GNLDSYLEVHRDILDGKVDLEPSGFRLDGRVWLGDGAALDPEAEVEGPAYIGENSRVE
                     AGARIREHTVLGKGVAVKSGAFLHRAVVHDNAYIGTSASLRGCVLGKNSDLKHGARLE
                     DGVVVSDECYVGEGAVISPGVKVYPFKTVEPGALVTSSIVWETRGARGLFGERGVSGL
                     VNIDVTPEMALRLGLAFGGSLPKTSIVVAGRDPTRTARVIKRAMVAGFNASAIDCHDL
                     ELIPVPVAKFYARTTRAHGGVAVRATPF"
BASE COUNT          343 a          770 c          835 g          396 t
ORIGIN      
        1 ggtcctggtc ggggaccgcg aggtcgcgcg actcggcgcc ggggagagct tcggcgagat
       61 ggcgctcctc gaccagggcg cacggtccgc ctcggtcgtc gcccaggagt cgatgacgct
      121 gtacgtcatc gatgcacagg acttcggacg gctcctcgag gatgtgccgt tcctcacgcg
      181 gaagatcctc cgtggagtct ccaaccggct gcgcagcgcc gagcaggcgc ccgcgtacgt
      241 ctggaaccgc ttctgaggcg gccggcgtga gccggccaga cacgacgagc gcgcccgtgc
      301 cggacgagcg aggcgctcgc cgcatcgtca ctgtcccgaa cgcgctctcg ctgttccgaa
      361 tcctcctcat ccccctgttc gtggtgctgc tggtcgggga gtcgacgcgc ctggccgggt
      421 tcgtcgtcct cgcggcggtc gtctcgaccg actgggtgga cggcgtcatc gcgcgtcgca
      481 cgggacaggt gaccgagatc gggaagctcc tcgacccgat cgcggatcgt ctcgcgctcg
      541 ccgccgcgct cgtcacgttc ctcgtgatcg acgtcttccc gctctgggcg gcgctcgccg
      601 tgctggtgcg cgacgcggcc gttctcttgg cggggctctg ggcgcttcgg agaggcatcc
      661 ggatcgacgt ccgaacgatc gggaagtacg cgacgttcac gctgatgtgg gggatcccga
      721 tgatcgcgtg ggggaacgcg ggaatgccgt tcgacgacct gatgctcgtc ctcggatggg
      781 tgtggttgcc cgtagggctc atcgagtact acgcggcctc ggtcgcatac gcgttcgacc
      841 tgcggaaggc gctccgcgcg cggcccgcct ctcggacggc ttcgtaggac ccgcggttat
      901 gattcgcacg tgaaggccgt cgtcatggcg ggcggccagg gaacccggct gcgcccgctc
      961 accagcaacc agccgaagcc gatgctgccg atcgtcgggc ggccgatgat ggagcacatc
     1021 ctgcggctgc tgaagacgca cggcttcgac gaggtcgtcg ccacagtgca gttcctcgcg
     1081 agcgtggtac ggacgtactt cggcgacggc tccgacctcg gcatctcgct ggcctacgcc
     1141 accgagaacg agccgctcgg caccgcgggc agcgtaaaga acgccgagca tttcctcgac
     1201 gacacgttcc tggtgatctc gggagacgcc gtcacggaca tcgatctccg cgcggcgacc
     1261 gagttccatc gtgagcgcga ggccgcggtg accgtggtgc tcaagcgctc gccgaacccc
     1321 ctcgacttcg ggatcgtgat caccgacgag gccggccggg tcgaccggtt cctggagaag
     1381 cccggctggg gtgaggtctt cagcgacacg atcaacacgg ggatctacgt gctcgacccc
     1441 gaggtgcttg cacacatccc cgcgggagag gatttcgact tcgccgccga cctgttcccg
     1501 ctcctactcg agaagggcat gccgctctac ggctacatcg cggaagggtt ctggaccgat
     1561 gtggggaatc tcgactccta tctggaggtc caccgcgaca tccttgacgg caaggtggat
     1621 ctcgagccgt ccgggttcag gctcgacggg cgcgtgtggc tcggcgacgg cgcggccctt
     1681 gaccctgagg ctgaggtcga gggtccggcg tacatcgggg agaactcgcg ggttgaggcc
     1741 ggcgcccgga tccgtgagca cacggtgctc gggaagggcg tcgcggtgaa atccggcgcc
     1801 ttcctgcatc gggctgtggt gcacgacaac gcgtacatcg ggacctccgc ctcgctgcgc
     1861 gggtgcgtgc tcgggaagaa ttccgacctc aagcacgggg cgcggctcga ggacggcgtc
     1921 gtcgtctcag acgagtgcta cgtcggcgag ggggcggtga tcagtcccgg cgtgaaggtg
     1981 tacccgttca agacggtcga gccgggtgcg ctcgtgacca gcagcatcgt ctgggagacc
     2041 cgcggcgctc gcggtctgtt cggtgaacgc ggcgtgtcgg ggctcgtcaa catcgacgtg
     2101 acgccggaga tggcgctccg tctcgggctc gcgttcggcg gcagcctccc gaagacctcg
     2161 atcgtcgtcg ccgggaggga cccgaccaga acggctcgag tgatcaagcg ggcgatggtc
     2221 gcggggttca atgcgagcgc gatcgactgc cacgatctcg agctcatccc ggtgcccgtc
     2281 gcgaagttct atgcgcgcac gacgcgggcg cacggaggcg tggccgtccg cgcgacgccg
     2341 ttcg
//
LOCUS       DASZOB010000435         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_130213, whole genome shotgun sequence.
ACCESSION   DASZOB010000435 DASZOB010000000
VERSION     DASZOB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130213"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VG709_06855"
     CDS             complement(<1..727)
                     /locus_tag="VG709_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HWC32831.1"
                     /translation="MAARQFLFTSESVTEGHPDKMADQISDSVLDAVLTDDPGGRVAC
                     ETLVTTGLVVVAGEITTKTYVDIPRLVRRRIREIGYDRAKYGFDADTCGVIVASDEQS
                     RDIAQGVEASYEVQHDPGGEDPLDRVGAGDQGMMFGFACDETEELMPLPIMLAHLITK
                     RLAEVRKAQVLPYLRPDGKAQVTIRYEVDEHGRQRPVEIERVLVSCQHRDGLDIDALV
                     KPDVVEHVLHPILPPDLYDEHRFQ"
     gene            complement(771..2285)
                     /locus_tag="VG709_06860"
     CDS             complement(771..2285)
                     /locus_tag="VG709_06860"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HWC32832.1"
                     /translation="MRPVHALAALVAASLVAAAGCGGDGAGDLASGAEVAPASAAFFL
                     SFDSDFDSDQWQAAEDLAAKFPGGRDALRSLPNKLEGNEDVDFENDVKPALGPEVDVV
                     LLDFEDEEAAVALTQPADEAKWRELVGKGDEPSVTEEIDGGWWAAAESQEALDRFKDA
                     RSDDSLAESDAFEQAMGELGDEALATAFVNGAALTAQLRQDAETTPDERQALECVLGG
                     GDVPSLAFGLAAEDDGFRLSGAFLTEGREAPESGPSDLAEELPAGALVFGSMHDLGRQ
                     VRDALRCVTDANDEARTQLMQAQLALGLSIEEDILPLFDGETAYAVYRQSESESQQGA
                     TPFGLTTITVVTDVEDEERAREVVDQIAVRASTFVDGVRVTDVDVGGIAAKRVTLPNE
                     GGGTVLYAVFDGKLVLTSAEEGLLGFTEDGPRLSEDEAYEAARDAAGAPDEAAGVLYA
                     NVGEFADLFLEAFMGLPGGPPSDTREALDPVRSLFLWGEADDDVTTIEGFVHVE"
     gene            complement(2323..>2914)
                     /locus_tag="VG709_06865"
     CDS             complement(2323..>2914)
                     /locus_tag="VG709_06865"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWC32833.1"
                     /translation="SGPNHAEEIAAGLPAAAVIACDDEELGVRLQHAVISPRFRVYMN
                     TDLVGVELCAAAKNVIALGAGAADGLGMGDNAKAALLTRGLAEMARLGDAAGARSETF
                     WGLAGMGDLTVTCWSRHSRNRRAGELIARGASAEEAAATIAMAVEGLTTAPVLRDLAQ
                     RLGIELPITDAVCRVLAGASAAEAVPALMERRPTGE"
BASE COUNT          382 a         1083 c         1026 g          423 t
ORIGIN      
        1 cctggaagcg gtgctcgtcg tacaggtcgg gagggaggat cgggtggagc acgtgctcga
       61 ccacgtccgg cttcaccagc gcgtcgatgt cgaggccgtc gcggtgctgg caggagacga
      121 gcacgcgctc gatctcgacc ggccgctggc gtccgtgctc gtcgacctcg taccggatcg
      181 tcacctgcgc cttgccgtcc ggccgcaggt acgggagcac ctgcgccttg cggacctccg
      241 cgaggcgctt cgtgatgagg tgcgcgagca tgatcgggag cggcatcagc tcctccgtct
      301 cgtcgcacgc gaacccgaac atcatcccct ggtcgccggc gccgacccgg tcgagcgggt
      361 cctcgccgcc gggatcgtgc tggacctcgt acgacgcttc cacgccctgc gcgatgtcgc
      421 gcgactgctc gtcgctcgcg acgatgacgc cgcacgtgtc ggcgtcgaac ccgtacttcg
      481 cgcggtcgta gccgatctcg cggatccggc ggcggacgag gcgcgggatg tcgacgtagg
      541 tcttcgtcgt gatctccccg gcgacgacga cgagtccggt cgtcacgagc gtctcgcacg
      601 cgacgcggcc gccggggtcg tccgtgagga cagcgtcgag gacggagtcc gagatctggt
      661 cggccatctt gtcgggatgg ccctcggtga cggactcgga ggtgaacagg aactggcggg
      721 cggccacgag cgctagagac tagacgaacc ccggatagcg gcgacgtcgc ctactcgacg
      781 tggacgaacc cctcgatcgt cgtcacgtcg tcgtcggctt cgccccagag gaacaacgac
      841 ctgaccgggt cgagcgcctc gcgggtgtcc gagggtgggc cgccgggcag gcccatgaag
      901 gcctcgagga acaggtccgc gaactcgccg acgttcgcgt agaggacgcc cgccgcctcg
      961 tcgggagcgc cggccgcgtc gcgcgccgcc tcgtacgcct cgtcctcgga aaggcgcggg
     1021 ccgtcctcgg tgaagcccag gaggccttcc tccgcggacg tgaggacgag cttgccgtcg
     1081 aagactgcgt agaggacggt ccctccccct tcgttcggca gggtcactcg cttcgcggcg
     1141 atgccgccga cgtccacgtc cgtcacgcgc acgccgtcca cgaacgtgct cgcccgcacg
     1201 gcgatctggt cgaccacctc gcgcgcgcgc tcctcgtcct cgacgtcggt cacgacggtg
     1261 atcgtcgtga ggccgaaggg cgtcgccccc tgctgcgact cgctctccga ctgccggtag
     1321 accgcatagg cggtctcccc gtcgaagagc ggaaggatgt cctcctcgat ggagagcccg
     1381 agcgcgagct gcgcctgcat gagctgcgtc cgcgcctcgt cgttcgcgtc cgtcacgcag
     1441 cgcagcgcgt cgcgcacctg gcggccgaga tcgtgcatcg acccgaagac gagcgcgccg
     1501 gcgggcagct cctccgcgag gtccgaaggc ccgctctcgg gcgcctcgcg gccctcggtc
     1561 aggaacgcgc cgctcaggcg gaacccgtcg tcctccgcgg cgaggccgaa ggcgagcgac
     1621 gggacgtcgc cgccgccgag cacgcactcg agcgcctgtc gctcgtccgg cgtcgtctcg
     1681 gcgtcctgcc gcagctgcgc ggtgagcgcg gcgccgttga cgaaggcggt cgcgagcgct
     1741 tcgtcgccaa gctcgcccat cgcctgctcg aaggcgtcgc tctccgcgag cgagtcgtcg
     1801 ctgcgcgcgt ctttgaaccg gtcgagcgcc tcctggctct cggcggcggc ccaccagccg
     1861 ccgtcgatct cctccgtcac ggagggctcg tcgcccttac ccacgagctc gcgccacttg
     1921 gcctcgtcgg cgggctgggt gagggcgacc gcggcctcct cgtcctcgaa gtcgagcagg
     1981 acgacgtcga cctcggggcc gagcgcgggc ttcacgtcgt tctcgaagtc cacgtcctcg
     2041 ttcccttcga gcttgttcgg aaggcttcga agcgcgtcgc ggccgccggg gaacttcgcg
     2101 gccaggtcct cggcagcctg ccactggtcg ctgtcgaagt cggagtcgaa cgagagaaag
     2161 aacgcggcag acgccggggc gacctctgcc ccggaggcga ggtcgcccgc gccgtccccc
     2221 ccgcaccctg ccgcggcgac gagcgacgcg gcgacgaggg cggcgagcgc gtgaacgggg
     2281 cgcacgctca cactgtagtc accgcacgcc tcggcgggcg cgtcactccc cggtcggacg
     2341 gcgctccatc agggccggga cggcttccgc ggcgctcgcc cccgccagca cccggcacac
     2401 cgcgtcggtg atcggaagct cgatgccaag gcgctgcgcg aggtcgcgga ggaccggtgc
     2461 cgtcgtgagg ccctcgaccg ccatcgcgat cgtcgccgcc gcctcctccg cggacgcgcc
     2521 gcgcgcgatc agctcgccgg cccggcggtt ccggctgtgc cggctccagc acgtcacggt
     2581 gaggtcgccc atcccggcga ggccccagaa cgtctccgag cgcgcgccgg ccgcgtcgcc
     2641 gagccgcgcc atctcggcga gcccgcgcgt gaggagggct gccttcgcgt tgtctcccat
     2701 gccgaggccg tccgccgcgc ccgcgccgag cgcgatcacg ttcttcgccg ccgcgcagag
     2761 ctccacgccg acgagatcgg tgttcatgta cacgcggaag cggggcgaga tgaccgcgtg
     2821 ctgcagccgc acgccgagct cctcgtcgtc gcaggcgatc accgccgcgg ccggcagccc
     2881 ggccgcgatc tcctcggcgt ggttaggccc ggag
//
LOCUS       DASZOB010000436         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933 k141_9853,
            whole genome shotgun sequence.
ACCESSION   DASZOB010000436 DASZOB010000000
VERSION     DASZOB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9853"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VG709_06870"
     CDS             complement(<1..292)
                     /locus_tag="VG709_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWC32834.1"
                     /translation="MTDTSMLATDPAAPFRDCPAEVVEAMGGVTPTVQQWDAISHPLR
                     SFAIVAGAGSGKTSLMAARIAYLTLARLGRVYAGHDGATPSETLALTFTNKAA"
     gene            complement(289..>1754)
                     /locus_tag="VG709_06875"
     CDS             complement(289..>1754)
                     /locus_tag="VG709_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWC32835.1"
                     /translation="DRRESARANRDLDALHAFGTSLARFRERRPWTHLAEYLDVVEQV
                     EFGPDPFVPPEERMPDAVRILSAHRAHGEEYDVVFVCGALEGELPSLSRARPMVSIED
                     LDAETSARGRFDERFREERALFRLAVSRGRRTVLTAYESRDSRRPRTPTRFLPPGAWT
                     IPAAGAPPTLAHAEAEHRRTRADRRAQPAARLASLAALAAIPARPETWWGALDWTHGP
                     PLHEGEFRTSYSRLSTLENCPLQYLYDTELGLDFSASHHMWLGSRVHQIIEEYWRGTI
                     ERSLETMERRLDELWRPEVFPSRAVEHRRRLDASTMFRKFLDSEAEVEPALVEHGFRF
                     PLDGAILRGRIDAVIAMPAGLRIVDYKTGRTPIPLKGADDDLQLGAYLLATRRDPELR
                     KLGNPHRMELVYLGTSDRGLVRRGTTPRPEDVERFEGRITGYLERIRAEEFAPNPEAA
                     CRNCAYRTLCPVWPEGADVPLHERTGAVPASDGEATR"
BASE COUNT          227 a          635 c          650 g          242 t
ORIGIN      
        1 ccgcggcctt gttcgtgaac gtcaacgcga gggtttccga cggcgtcgcg ccgtcgtgcc
       61 ccgcgtagac gcgcccgagc cgcgccagcg tgaggtacgc gatccgggcc gccatcagcg
      121 acgtcttgcc ggagccggcg ccggcgacga tcgcgaacga ccggagcggg tggctgatcg
      181 cgtcccactg ctggaccgtc ggcgtgacgc cgcccatcgc ctcgacgacc tcggccgggc
      241 agtcgcggaa cggagcggcc gggtcggtgg ccagcatcga cgtgtcggtc atcgcgtcgc
      301 ctcgccgtcg gacgccggaa ccgcgccggt gcgctcgtgc agcgggacgt ccgcgccctc
      361 gggccagacg gggcacagcg tccggtacgc gcagttgcgg cacgccgcct cggggttcgg
      421 tgcgaactcc tcggcgcgga tgcgctcgag gtagccggtg atgcgtccct cgaaacgctc
      481 gacgtcctcc ggccgcggcg tcgtgccgcg acgaaccagg ccgcgatcgg acgtcccgag
      541 gtagacgagc tccatgcggt gcgggttgcc gagcttccgc agctcggggt cgcggcgcgt
      601 cgccagcagg tacgcgccga gctgcaggtc gtcgtccgcc cccttgagcg ggatcggcgt
      661 tcggccggtc ttgtagtcga cgatccgcag gcccgcgggc atcgcgatga cggcgtcgat
      721 gcgccccctc aggatcgcgc cgtcgagcgg gaaccggaac ccgtgctcga cgagcgccgg
      781 ctccacctcc gcctcggagt cgaggaactt ccggaacatc gtcgaggcgt ccagccgccg
      841 tcgatgctcg accgcgcggc tcgggaacac ctcggggcgc cagagctcgt cgaggcgccg
      901 ctccatcgtc tcgaggctcc gctcgatcgt gccccgccag tactcctcga tgatctggtg
      961 gacgcgagat cccagccaca tgtggtggct ggcgctgaag tcgagcccga gctcggtgtc
     1021 gtagaggtac tggagcgggc agttctcgag cgtcgacagc cggctgtacg aggtcctgaa
     1081 ctcgccctcg tggagcggcg gtccgtgcgt ccaatcgagc gcgccccacc aggtctccgg
     1141 ccgcgcgggg atcgcggcga gcgccgcgag cgaggcgagc cgggcggccg gctgcgcgcg
     1201 gcggtccgcg cgcgtgcggc gatgctcggc ctcggcgtgc gcgagggtcg gtggcgcacc
     1261 ggccgcgggg atggtccatg cgccgggcgg taggaaccgc gtcggcgtcc gaggccggcg
     1321 ggagtcgcgc gactcgtagg ccgtgagcac ggtcctgcgc ccgcgcgaga ccgccaggcg
     1381 gaacagcgcc cgctcctcgc ggaaacgctc gtcgaaccgc cctcgcgcgc tggtctcggc
     1441 gtcgaggtcc tcgatgctca ccatcggccg tgcccgcgac agcgacggta actccccctc
     1501 gagcgctccg cagacgaaca caacgtcgta ctcctcgccg tgcgcgcggt gagccgagag
     1561 gatccgcacc gcgtccggca tccgttcctc cggcggaacg aacggatccg ggccgaactc
     1621 cacctgctcg accacgtcga ggtactcggc gaggtgcgtc cagggccgcc gttcgcggaa
     1681 cctcgcgagc gaggtcccga acgcgtggag cgcgtcgagg tcgcggttcg cgcgggcgga
     1741 ctcgcgccgg tccg
//
LOCUS       DASZOB010000437         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_150657, whole genome shotgun sequence.
ACCESSION   DASZOB010000437 DASZOB010000000
VERSION     DASZOB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150657"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VG709_06880"
     CDS             <1..607
                     /locus_tag="VG709_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWC32836.1"
                     /translation="TEGLERLGVRVEAAVRDGPTRRAFTFIDANGERTITTIGERLAP
                     SADDPLRWSDLAATDAVYLTAGDRGAMAAARRARVVVATARILRDLAAAHLELDALVG
                     SARDPSERYEEGALMPPPRLVVLTEGRDGGTYRTAEGRSGRFPATPPPGPLVDTYGAG
                     DSFAAGLAYGLAAERDDLSAALDVASRCGAEATTRRGAHGA"
     gene            complement(627..>1786)
                     /locus_tag="VG709_06885"
     CDS             complement(627..>1786)
                     /locus_tag="VG709_06885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWC32837.1"
                     /translation="RGVAFHHAEIGGLEPGTTYFYQVFSDGVPGRYDRYHPGSFTSLR
                     PPAAKPLFEFAILADIHIGEDVSGSPAVPTYRASPEDDYSTAMARAAVAEINGRGVDF
                     TMLPADNSNHGSRHDLEDAKAILDGLERPYVIARGAHDRPKQYEEAVEECGADGDCFR
                     AVFFPERPAGAQPQHIYYAYDTGRKSRYVVIALDSANLDTGTGELSDEQLAWLDRKLT
                     ETDRGNVPVFIFFHHPVSEWSTTLAVPPLVFGVNQQDAQEFLQIVGRHDSVRTIVNSH
                     THRNWISYSPWTGRVPILEVGPSKEYPGGYSIIRAYRDGFTRTFHRLRCEFCRRWIET
                     TRWEYAGNYPHYTTGSLRDRNFVHRWDRPDVPAGPPSLPFNPWPPFVPMEA"
BASE COUNT          252 a          609 c          665 g          260 t
ORIGIN      
        1 gaccgagggg ctcgagcggc tcggcgtccg cgtcgaggcg gccgtccgcg atggcccgac
       61 gcgccgcgcg ttcacgttca tcgacgcgaa cggcgagcgt acgatcacca cgatcggcga
      121 gcggctcgcg ccgtcggccg acgacccgct ccggtggagc gacctcgcgg cgaccgacgc
      181 cgtctacctc accgccggcg accggggggc gatggccgcc gcgcggaggg cccgcgtcgt
      241 cgtcgcgacc gcccggatcc tgcgcgacct cgcggccgcg cacctggagc tcgacgcgct
      301 ggtgggcagc gcccgcgacc cgagcgagcg atacgaggag ggggcgctga tgccgccacc
      361 gcgcctcgtg gtgctgaccg agggccgcga cggcggaacg taccgcacgg ccgaggggcg
      421 ctccggccgc ttccccgcca cgccgccgcc cgggccgctc gtcgacacgt acggtgcggg
      481 cgactcgttc gcggcaggac tcgcgtacgg gctcgccgcg gaacgggacg acctctccgc
      541 ggcactcgac gtcgcgtcgc gatgcggggc ggaggcgacg actcgtcgcg gcgcgcacgg
      601 cgcgtaggcg cgtgtgggat cggcggctac gcctccatgg ggacgaacgg cggccaggga
      661 ttgaacggta ggctcggggg acctgctggg acgtccgggc ggtcccaccg gtgcacgaag
      721 ttgcggtcgc gcaggctgcc cgtcgtgtag tgcggatagt tccccgcgta ctcccagcgc
      781 gtcgtctcga tccatcggcg gcagaactcg caccggagcc ggtggaacgt ccgcgtgaag
      841 ccatcgcggt acgcgcggat gatcgagtag ccgcccgggt actccttcga cgggccgacc
      901 tcgaggatcg ggacgcgtcc ggtccagggc gagtagctga tccagttccg gtgcgtgtgc
      961 gagttgacga tcgttcgcac gctgtcgtgg cggccgacga tctggaggaa ctcctgcgcg
     1021 tcctgctgat tgacgccgaa cacgagcgga ggcacggcga gcgtcgtcga ccactccgag
     1081 accgggtggt ggaagaagat gaagaccggg acgttgccac ggtccgtctc cgtgagcttc
     1141 cgatcgagcc acgcgagctg ctcgtccgag agctcgccgg ttcctgtgtc cagattcgcg
     1201 gagtccagcg cgatcacgac gtatctcgac ttcctcccgg tgtcgtacgc gtagtagatg
     1261 tgctggggct gcgcgccggc cggacgctcc gggaagaaca cggcgcggaa gcagtcgccg
     1321 tcggcgccgc attcctccac ggcttcctcg tattgcttgg ggcggtcgtg cgcgccacga
     1381 gcgatcacgt acgggcgctc gaggccgtcg aggatcgcct tcgcgtcctc gaggtcgtgc
     1441 cgcgacccgt ggttggagtt gtcggcggga agcatcgtga agtcgacgcc acggccattg
     1501 atctcggcga ccgccgctcg agccatcgcc gtcgagtagt cgtcctccgg cgacgcgcgg
     1561 tacgtcggca cggcggggct tccggagacg tcctcgccga tgtggatgtc cgcgaggatc
     1621 gcgaactcga acaggggctt ggccgccggc ggacgaaggc tcgtgaacga accggggtgg
     1681 tagcggtcgt accgacccgg gacaccgtcc gagaacacct ggtagaagta cgtcgtcccg
     1741 ggctcgagcc cgccgatctc ggcgtggtgg aacgcgacgc cccgct
//
LOCUS       DASZOB010000438         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_90202, whole genome shotgun sequence.
ACCESSION   DASZOB010000438 DASZOB010000000
VERSION     DASZOB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90202"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VG709_06890"
     CDS             complement(<1..730)
                     /locus_tag="VG709_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWC32838.1"
                     /translation="MATVADRVKRVLVGRRIASAKAEHQLLPKTLALPVFSSDPLSSN
                     AYATEEMMLVLVAAGAVALNVMLPIAAVIAVVLVIVVTSYRQTVRAYPRGGGSYIVAR
                     ENLGTVPGLVAASAILIGYVVTAAVSATAGAIAVTSAAPGLAEHRITLALGFILLIAL
                     ANLRGVRESGLLFAVPTYGFVLSIYVLLIAGFIRCLGGCPVAETADLPLHAEQAALGA
                     FLILRAFTQGSSALTGVEAIADGVQ"
     gene            806..1462
                     /locus_tag="VG709_06895"
     CDS             806..1462
                     /locus_tag="VG709_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWC32839.1"
                     /translation="MHIVIMGCGRVGAELTIQLSKGGHTVCIIDKRQQAFERLPPDFN
                     AKTIVGIGFDREVLEEAGIKEADAFIAVSNGDNSNIVSARIAREHYKVPNVIARIYDP
                     RRAEIYERLDIPTIASVRWAARQIEYLLFHGKEQMRGVLGGGSLLHIQVQAPEHMVGK
                     RVETVTADGEVEVIGVDRGGTGFIPKADSTFQQGDIVHFIVHESSMEKLDLLLEPIAE
                     "
     gene            1473..2144
                     /locus_tag="VG709_06900"
     CDS             1473..2144
                     /locus_tag="VG709_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWC32840.1"
                     /translation="MRVVVAGAGNIGRHLANDLHKRGHDVTVIEQDPAVLARARGDAP
                     SGVDIVHGDPCEPWVLDKGDVRSCDVIVAATGDDEDNLVISLLSKQEYAVPAVVARVN
                     HPKNEWLFTEQWGVDVAMSPPHVLTALVEEAVTVGELVRLFKLERGNVTLVELTIPEA
                     STIAGRPLYELRLPQDSAIVAILRQGHVVIPQPETQLTAGDEIFAIATPETEEPLRAA
                     VLGGD"
     gene            complement(2149..2505)
                     /locus_tag="VG709_06905"
     CDS             complement(2149..2505)
                     /locus_tag="VG709_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HWC32841.1"
                     /translation="MGWFRRWLERFRQSDEERLTDEVREWAESIPQTSRIDQCPSREH
                     VRVAGAVRRLTIRPVEGFYSLEALVSDGTGEILVRWMGRHRIPGLGLGTRIVLEGVLG
                     RDRDGLRMVNPQFEFG"
     gene            complement(2510..>3105)
                     /locus_tag="VG709_06910"
     CDS             complement(2510..>3105)
                     /locus_tag="VG709_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWC32842.1"
                     /translation="NQFREHPGIMAGTEKPDYGRVVDICTRTSLRELLTPGLLAVLAP
                     IFVGFALEAEALGAYLAGAILTGQLLAVMLSNAGGAWDNAKKLVEEGLYGGKGSDSHK
                     ATVIGDTVGDPFKDTAGPALNPLIKVMNLVALLIAPIVVASADDTGLRIAVSVGIFLV
                     LGVAIWISKRVKASDLLTVAEPASPEAAAVTTEAPGA"
BASE COUNT          514 a         1066 c         1102 g          423 t
ORIGIN      
        1 cctgtactcc gtcggcgatc gcctcgacgc cggtgagcgc cgacgatccc tgcgtgaacg
       61 cccggaggat gaggaaggca cccagcgcgg cttgctccgc gtggagcggg aggtcggcgg
      121 tctcggcgac cggacacccg ccgaggcagc ggatgaaccc tgcgatgagg agcacgtaga
      181 tcgacagcac gaacccgtac gtcgggacgg cgaacaggag tcccgactcc cgcacccctc
      241 ggaggttcgc aagcgcgatg aggaggatga acccgagcgc gagcgtgatg cggtgctcgg
      301 cgagccccgg cgcggcggac gtcacggcga tcgcgcccgc ggtcgcgctc acggcggcgg
      361 ttacgacgta cccgatgagg atcgccgagg ccgcaacgag accgggaacg gtcccgaggt
      421 tctcgcgagc gacgatgtac gagccgccgc cgcgcgggta cgcgcggacg gtctggcggt
      481 acgacgtgac gacgatcacg agcacgaccg cgataacggc cgcgatcgga agcatcacgt
      541 tgagcgcgac ggcgcccgcg gcgacgagga ccagcatcat ctcctccgtc gcgtaggcgt
      601 tcgacgagag cgggtccgac gagaagacgg ggagcgcgag ggtcttcggg agcagctggt
      661 gctcggcctt cgccgacgcg atgcgccgcc cgacgaggac gcgcttcacc cgatcggcga
      721 cggtcgccac gtctcgattc tagggagccg tacggcgacc gccacacgag gttgtgctcc
      781 gcccgcacgg gggtacgctt gcgtcgtgca catcgtgatc atggggtgcg gccgcgtcgg
      841 agccgagctc acgatccagc tctcgaaggg cggtcacacg gtctgcatca tcgacaagcg
      901 gcagcaggcc ttcgagcggc tgccgcccga cttcaacgcg aagacgatcg tcgggatcgg
      961 cttcgatcgc gaggtgctcg aggaggccgg catcaaggag gccgacgcgt tcatcgcggt
     1021 ctcgaacggc gacaactcca acatcgtctc ggctcgcatc gcccgcgagc actacaaggt
     1081 cccgaacgtc atcgcgcgga tctacgaccc tcgccgagcc gagatctacg agcggctcga
     1141 catccccacg atcgcgagcg tccgatgggc ggcgaggcag atcgagtacc tgctgttcca
     1201 cggcaaggag cagatgcgcg gcgtgctcgg cgggggctcg ctgctccaca tccaggtcca
     1261 ggcgccggag cacatggtcg ggaagcgcgt cgagaccgtg acggccgacg gggaggtcga
     1321 ggtgatcggc gtcgaccgcg gcggaacggg gttcatcccg aaggccgact cgacgttcca
     1381 gcagggggac atcgtccact tcatcgtcca cgagagctcg atggagaagc tcgacctgct
     1441 cctcgagccg atcgccgagt gaggacggac gcgtgagggt cgtcgtcgcc ggcgcgggga
     1501 acatcgggcg ccacctcgcg aacgacctcc acaagcgtgg gcacgacgtc acggtgatcg
     1561 agcaggatcc ggccgtgctc gccagggcgc gcggcgacgc gccgagcggc gtcgacatcg
     1621 tccacggcga cccgtgtgag ccctgggtcc tcgacaaggg cgacgtccgc tcgtgcgacg
     1681 tgatcgtcgc cgccacgggg gacgacgaag acaacctcgt gatctcgctg ctctcgaagc
     1741 aggagtacgc ggtcccggcc gtcgtggcgc gcgtcaacca cccgaagaac gagtggctgt
     1801 tcacggagca gtggggcgtc gacgtcgcga tgagcccgcc gcacgtcctg acggccctcg
     1861 tcgaggaggc cgtcacggtc ggcgagctcg tccggctctt caagctcgag cgaggcaacg
     1921 tgacgctcgt cgagctcacg atcccggagg cctcgacgat cgcggggcga ccgctctacg
     1981 agcttcggct tccgcaggac tcggcgatcg tcgcgatcct gcggcagggg cacgtcgtga
     2041 tcccgcagcc ggagacgcag ctcactgccg gcgacgagat cttcgcgatc gcgacgcccg
     2101 agaccgagga gccgctccgg gccgcggtgc tcggcgggga ctagcggcct agccgaactc
     2161 gaactgcgga ttgaccatcc gaagcccgtc ccggtcgcgg ccgaggacgc cctcgagcac
     2221 gatgcgtgtc ccgaggccga gcccggggat gcggtggcgt cccatccagc gcaccaggat
     2281 ctcgccggta ccgtcggaga cgagcgcctc gagcgagtag aagccctcga ccggacggat
     2341 cgtcagcctg cgcacggcgc ccgcgacgcg gacgtgctca cggctcgggc actgatcgat
     2401 ccgggaggtc tgcgggatgc tctcggccca ctcgcgaacc tcgtcggtca ggcgctcctc
     2461 gtcgctctgc cggaagcgct cgagccaccg gcggaaccag cccacgcggc taggcgcccg
     2521 gcgcctctgt ggtgacggcg gccgcctccg ggctcgctgg ctccgcgacc gtgaggaggt
     2581 ccgacgcctt cacgcgcttc gagatccaga tcgcgacgcc gaggacgagg aagatcccga
     2641 cggagaccgc gatccgcagg ccggtgtcgt cggccgacgc gacgacgatc ggtgcgatca
     2701 gcagcgccac gaggttcatg accttgatca gcgggttcag cgccggccct gccgtgtcct
     2761 tgaagggatc acccacggtg tcgccgatga ccgtcgcctt gtgcgagtcg gagcccttcc
     2821 cgccgtagag cccctcctcg acgagcttct tcgcgttgtc ccaggcgccg cccgcgttcg
     2881 acagcatcac ggcgaggagc tgcccggtga ggatcgcgcc ggcgaggtac gcgccgagcg
     2941 cctcggcctc cagcgcgaac ccgacgaaga tcggcgcgag caccgcgagc agccccggcg
     3001 tgagtagctc ccgcagcgac gtccgagtgc agatgtcgac gacgcgtccg tagtccggct
     3061 tctcggtccc ggccatgatg ccagggtgct cgcggaactg gttcc
//
LOCUS       DASZOB010000439         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_130429, whole genome shotgun sequence.
ACCESSION   DASZOB010000439 DASZOB010000000
VERSION     DASZOB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130429"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VG709_06915"
     CDS             complement(<1..2053)
                     /locus_tag="VG709_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWC32843.1"
                     /translation="MRLRAQPGEQLDRYEETTFFLDGKPITAFGGDTIGSALFANGQR
                     TFSRSFKYHRPRGLLCCSGHCAHCMMTVDGVPNVRVCVEPVREGARVSAQNVRGSLDH
                     DLMAVTDKVGGPFTPVGFYYRTMIRPRRAWPLYERFLRSAAGLGAVDKHARRSRRYDT
                     ENRHVDVLVIGGGSAGIEAAREAAARGEDVVVVDEGPDPAPGDLAGEPFETLAPARAL
                     GIYEGNLVPVDAGSILYRFRAGRIVVATGAVEQPLLFPGNDLVGVLLPNAVRRLVRAW
                     SLKPGDRAVVIAADERALDVLHDLEAAGTEVARVVDLRREKIREIVARGRKGRVRSVA
                     LDGDAVACDVVVMSGGRQPAYSLLAQAGARVELDGAHGVFVPTELPQGIEAVGSVAGA
                     GLDGAAVPPATFNGAAKADKCFTCICEDVTDKDVKRAIAEGFDSIELAKRYTTVTMGP
                     CQGKLCQLPSIRLYARENETTAAEIGATTARPPWAPVSLGLLAGRGHEPAKRTSIHHR
                     HKELGARMMWTGAWRRPHSYGDPKAEAKAVHESLGLIDVSTLGKLILRGSDAGAFLDR
                     LYPNRFSDLKVDRVRYGVLGTDGGRIMDDGTIARLDEETFYVTTTSTGADTVIEWFEW
                     WNAVWHLDVDVTNVTGALAAVNVAGPCARDVMAQLTDLDVSNEAFKYLDARRAHVAGV
                     PS"
     gene            complement(2050..>2383)
                     /locus_tag="VG709_06920"
     CDS             complement(2050..>2383)
                     /locus_tag="VG709_06920"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HWC32844.1"
                     /translation="LTTEHMSFLLPCPNCGPRDVNEFAYQGEITTRPQESPSFRELTS
                     YLYFRRNVAGVQREWWFHRLGCQAWFHAERDTRTNEVLLTELAKPAADEAAPGPQATP
                     ALGDTPTL"
BASE COUNT          333 a          843 c          861 g          346 t
ORIGIN      
        1 gcgacgggac gcctgcgacg tgggcgcgcc gcgcgtccag atacttgaac gcctcgttcg
       61 agacgtcgag gtccgtgagc tgcgccatga cgtcgcgcgc gcaggggccg gccacgttga
      121 cggccgcgag cgcgccggtc acgttcgtga cgtcgacgtc taggtgccag acggcgttcc
      181 accactcgaa ccactcgatg acggtgtccg cgccggtcga ggtcgtggtg acgtagaacg
      241 tctcctcgtc gagccgcgcg atcgtgccgt cgtccatgat cctgccgccg tcggtgccga
      301 ggacgccgta gcggacgcgg tcgaccttca ggtcggagaa gcgattcggg tagaggcggt
      361 cgaggaacgc gcccgcgtcg gagccgcgga ggatcagctt gccgagcgtc gagacgtcga
      421 tcaggccgag agactcgtgc accgccttcg cctcggcctt cgggtcgccg tacgaatgcg
      481 gcctccgcca cgcgcccgtc cacatcatgc gggcgccgag ctccttgtgg cggtggtgga
      541 tggacgtccg cttggcgggc tcgtgtccgc gtccggcgag gagcccgagc gacaccggcg
      601 cccacggtgg ccgcgccgtc gtcgccccga tctccgcggc cgtcgtctcg ttctctctcg
      661 cgtagagccg gatcgaggga agctggcaga gcttcccctg gcacggcccc atcgtcacgg
      721 tcgtgtagcg cttcgccagc tcgatcgagt cgaagccctc ggcgatcgca cgcttcacgt
      781 ccttgtcggt cacgtcctcg cagatgcacg tgaagcactt gtccgccttc gcggcgccgt
      841 tgaatgtcgc gggcgggacg gccgcgccgt cgagccccgc gccggcgacg gatccgactg
      901 cctcgattcc ctgcggaagc tcagtgggga cgaagacgcc gtgggctccg tcgagctcga
      961 cgcgcgcgcc ggcctgggcg aggagcgagt aggcaggctg gcgccctccg gacatgacga
     1021 cgacgtcgca agcgacggcg tcgccgtcga gcgcaacaga gcgaacgcgg cccttgcggc
     1081 cgcgcgcgac gatctcgcga atcttctccc tccggaggtc gacgacgcgc gcgacctccg
     1141 tgcccgcagc ctccaggtcg tggagcacgt cgagcgcccg ctcgtccgcc gcgatgacca
     1201 cggcacgatc ccccggcttg agcgaccacg ccctgaccag ccgccgcacc gcgttcggca
     1261 ggaggacacc gacgaggtcg ttgccgggga agaggagcgg ctgctcgacc gcgccggtcg
     1321 cgacgacgat ccgccccgcc cggaagcggt agaggatgga gccggcgtcg acggggacga
     1381 ggttcccctc gtagatccca agcgcgcgcg cgggagcgag cgtctcgaac ggctcgccgg
     1441 cgaggtcgcc gggcgccggg tctgggccct cgtcgacgac gacgacgtcc tcgccccgcg
     1501 ccgccgcctc ccgtgcggcc tcgatcccgg cgctgccgcc gccgatcacg agcacgtcca
     1561 cgtgccggtt ctcggtgtcg tagcggcgcg agcgcctcgc atgcttgtcg acggcgccga
     1621 ggcccgccgc gctgcggagg aaccgctcgt agagcggcca cgcacgccgc ggccggatca
     1681 tcgtgcggta gtagaagccg accggcgtga acgggccgcc gaccttgtcc gtcaccgcca
     1741 tcaggtcgtg gtcgagcgag ccgcgcacgt tctgcgcgga gacgcgcgct ccctcgcgca
     1801 ccggctcgac gcacacgcgg acgttcggga cgccgtccac cgtcatcatg cagtgcgcgc
     1861 agtggcccga gcagcagaga agcccgcgcg gccggtggta cttgaagctg cgcgagaagg
     1921 tgcgctgccc gttcgcgaag agcgccgacc cgatcgtgtc gccgccgaag gccgtgatcg
     1981 gctttccgtc gaggaagaag gtcgtctcct cgtagcgatc gagttgctcg ccgggctgcg
     2041 cgcggagcct cacagcgtcg gcgtgtctcc caaggcggga gtcgcctgcg gacccggggc
     2101 ggcctcgtcc gcggccggct tcgcgagctc cgtgaggagg acctcgttcg tcctagtgtc
     2161 gcgctccgcg tggaaccacg cctgacaccc gaggcggtgg aaccaccact cgcgctggac
     2221 gcccgcgacg ttgcggcgga agtagaggta cgaggtgagc tcgcggaagc tcggcgactc
     2281 ctgcgggcgg gtcgtgatct cgccctggta ggcgaactcg ttcacgtcgc gcgggccgca
     2341 gttggggcag ggaaggagga aggacatgtg ctcggtcgtc agc
//
LOCUS       DASZOB010000440         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_119833, whole genome shotgun sequence.
ACCESSION   DASZOB010000440 DASZOB010000000
VERSION     DASZOB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119833"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VG709_06925"
     CDS             complement(<1..1710)
                     /locus_tag="VG709_06925"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWC32845.1"
                     /translation="MSDANIERRDPLDALRDPSRGSAILPFWFLNGDLDPQEMRWQLR
                     ELRDKGMRGVILHGRYGLETPYLGPEYLDRIRLATEEATGLGLETWVYDEMNWPSGTA
                     DKRVLKERPDLAQRYIECVSFTIEGPWFMCLTGEDSRYLDFERSDPVAAFAIGPGGEV
                     VDLTRNLSFEKVVPWEVPPGTWRLSYVVEKRADYYIDAMDPESTREFLRLGYAPYADA
                     LGPLAARLVGFYSDEPAMHYYLTAGDNPVVPWTKGMFRRFHERNGYDLRSRLADLFFD
                     VSPDSARVRFDYYSTTTELYTNAYYRQIHDWCRERGFRFVAHLLYEEWLRRMIRVEGN
                     LFKHYRHMDVVGVDHLYPVIGTREQPDQHVAMKVASSAAHHYGTERVLCESFGGIFMD
                     ATMQRMKWIADWEYVLGVNVLNPHGFHYTLEGARKRDWPPSMFYQYPWWRYYEGFNTY
                     IARLSEVLSGGRHVAKVAVLWPINAMFATYTPQTRSPLGDRTESDTNVLTDLLLRLHH
                     DFDYLDEDVLADAEVSDGRIRIADEEYEVLLLPPMAHLKLVTVEALERLVASGGKALG
                     MVFL"
     gene            complement(1707..>1955)
                     /locus_tag="VG709_06930"
     CDS             complement(1707..>1955)
                     /locus_tag="VG709_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HWC32846.1"
                     /translation="GPPRTLRLGDWNWMAYRIVNAHFREVATIMRGMRIGMRLLASGR
                     LSMDGLVTHRFSLEAIDEAFRTAIDKPPGFVKATVEPR"
BASE COUNT          321 a          660 c          658 g          316 t
ORIGIN      
        1 aaggaacacc atcccgagcg ccttgccgcc cgacgccacg agacgctcca gcgcctcgac
       61 ggtcacgagc ttcaaatggg ccatcggcgg cagcaggagg acctcgtact cctcgtccgc
      121 gatccggatc cgcccatcgg acacctcggc gtccgcgagc acgtcctcat cgaggtagtc
      181 gaagtcgtgg tgcaggcgga ggagcaggtc cgtgagcacg ttcgtgtccg actcggtccg
      241 gtcgccgagc ggcgagcggg tctgcggcgt gtacgtcgcg aacatcgcat tgatcggcca
      301 gagcaccgcc accttcgcga cgtgacggcc gccgctgagc acctcggaga gccgggcgat
      361 gtaggtgttg aaaccctcgt agtaccgcca ccacgggtac tggtagaaca tcgacggcgg
      421 ccagtctcgc ttgcgcgcgc cctcgagcgt gtagtggaac ccgtgcgggt tgagcacgtt
      481 gacgcccagc acgtactccc agtcggcgat ccacttcatg cgctgcatcg tcgcgtccat
      541 gaagatgccg ccgaacgact cgcacagcac ccgctcggtg ccgtaatggt gcgcggcgga
      601 gctcgccacc ttcatcgcga cgtgttggtc gggctgctcg cgcgtcccga tgaccgggta
      661 cagatggtcg acgccgacga cgtccatgtg ccggtagtgc ttgaagaggt tcccctccac
      721 gcggatcatc cgccgcagcc actcctcgta gaggaggtgg gcgacgaacc ggaatccgcg
      781 ctcgcggcac cagtcgtgga tctgccggta gtacgcgttc gtgtacagct cggtcgtggt
      841 cgagtagtaa tcgaagcgca cgcgcgccga gtcgggcgag acgtcgaaga acaggtccgc
      901 gaggcgtgac cggaggtcgt agccgttgcg ctcgtggaaa cgccggaaca tgcccttggt
      961 ccacgggacg acggggttgt cgccggcagt gaggtagtag tgcatcgcgg gctcgtcgga
     1021 gtagaagccg acgagccggg cggcgagcgg tccgagcgcg tcggcgtacg gcgcataccc
     1081 gaggcgcagg aactcgcgcg tcgactcggg gtccatcgcg tcgatgtagt agtcggcgcg
     1141 cttctcgacg acgtacgaga ggcgccacgt ccccggcggg acctcccagg gcacgacctt
     1201 ctcgaacgag aggttgcggg tgagatcgac gacctcgccg ccgggaccga tcgcgaacgc
     1261 cgcgacgggg tccgagcgtt cgaaatcgag gtaccggctg tcctcgccgg tcaggcacat
     1321 gaaccaggga ccctcgatcg tgaagctcac gcactcgatg tatcgctggg caaggtcggg
     1381 ccgctccttc aggacccgct tgtcggccgt tcccgacggc cagttcatct cgtcgtacac
     1441 ccaggtctcg agcccgagcc ccgtcgcctc ctccgtcgcg agccgtatgc ggtcgaggta
     1501 ctccggcccc aggtacggcg tctcgagccc gtaccggccg tggaggatca cgccccgcat
     1561 ccccttgtcg cggagctcgc ggagctgcca gcgcatctcc tgagggtcga ggtcgccgtt
     1621 caggaaccag aacggcagga tcgccgaccc ccgtgaggga tcgcgcagcg cgtccagcgg
     1681 gtctcgtcgt tcgatgttcg cgtcgctcat cggggctcca ccgtcgcttt cacgaagccg
     1741 ggcggcttgt cgatcgccgt ccgaaacgcc tcatcgatcg cttccaggga gaagcggtgc
     1801 gtcaccaggc cgtccatgga gagacgcccc gaggcgagca gccgcatgcc gatccgcatg
     1861 ccgcgcatga tggtcgcgac ctcgcggaag tgcgcgttca cgatccggta cgccatccag
     1921 ttccagtctc cgagtcgcag cgtccgcggc ggccc
//
LOCUS       DASZOB010000441         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_100638, whole genome shotgun sequence.
ACCESSION   DASZOB010000441 DASZOB010000000
VERSION     DASZOB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100638"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="VG709_06935"
     CDS             complement(<1..>1725)
                     /locus_tag="VG709_06935"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HWC32847.1"
                     /translation="GEYERLLKDLAAKGFARARIDGEVRELSEPIRLDRYYKHTIEVV
                     VDRLVAKPGIRRRLADSVETALQLAEGMAAVAIQTDGKEDIIPFSENLACVHCGLSFD
                     ELAPRNFSFNSPYGACETCDGLGTRLEVDPELVVPDKDLAITEGAIAPWASTRLEYWN
                     RVIAAVADAHGFSLKTPWRKLPDEAREVVLYGSDKDIYVRYRNRYGRQRAYWTVFEGV
                     IPNIERRHEETDSDHARWRWEQFMREVPCRTCKGARLRPESLAVTIDGVNISQLTDMS
                     IVATLRFLEQLEPKLSERERMIGERLLKEIRGRLGFLVDVGLDYLTLGRSAGTLAGGE
                     AQRIRLATQIGSGLVGVLYILDEPSIGLHQRDNKRLIDTLVRLRDLGNTLIVVEHDES
                     TIRTADWIVAIGPGAGEAGGEVVYAGPVEGIETTEGSITGAYLAGRRTIPMPEVRRRP
                     GGRWLEVRGAREHNLKGIDVDIPRESLTVITGLSGSGKSSLAFDTIYAEGQRRYVESL
                     SAYARQFLEMMQKPDVDHIEGLSPAISIEQKTTSRNPRSTVATVTEMYDYMRLLWARV
                     GVPYSPAPG"
BASE COUNT          248 a          603 c          594 g          280 t
ORIGIN      
        1 gccgggggcc gggctgtatg gcactccgac gcgcgcccag agcaggcgca tatagtcgta
       61 catctcggtg acggtggcga cggtcgagcg cgggttgcgg ctggtagtct tctgctcgat
      121 cgagattgcg ggggagaggc cctcgatatg atcgacgtcg ggcttctgca tcatctcgag
      181 gaactggcgc gcataggcgc tcagggattc gacgtagcgg cgctgcccct cggcatagat
      241 ggtgtcgaag gcgagcgagg atttgcccga ccccgacagg ccggtgatca ccgtcaggct
      301 ctcgcgcggg atgtcgacgt cgatgccctt gagattgtgc tcgcgcgcgc cgcggacctc
      361 gagccagcgg ccgccgggcc gacgccggac ctccggcatg gggatcgtcc gccgtccggc
      421 gaggtacgcg cccgtgatcg agccctcggt cgtctcgatc ccctccaccg ggcccgcgta
      481 cacgacctcg ccccccgcct cgccggcgcc gggaccgatg gccacgatcc agtccgcggt
      541 acggatcgtg gactcgtcgt gttcgaccac gatcagcgtg ttgccgaggt cgcgcagacg
      601 cacgagcgta tcgatgagcc gcttgttgtc tcgctggtgg agcccgatcg atggctcgtc
      661 gaggatgtac agcacgccga cgagcccgga gccgatctgc gtcgcgagcc ggatccgctg
      721 cgcctcgccg ccggccagcg tcccggcgga gcggcccagc gtcaggtagt ccaggccgac
      781 gtcgacgagg aagccgagcc ggccccggat ctccttgagg agccgctcgc cgatcatccg
      841 ctcgcgttcc gacagcttgg gctcgagctg ctcgaggaac cgcagcgtcg cgacgatcga
      901 catgtcggtg agctgcgaga tgttgacccc gtcgatcgtg acggcgagcg actcggggcg
      961 cagccgcgcg cccttgcacg tgcggcacgg cacctcgcgc atgaactgct cccaccgcca
     1021 ccgcgcgtgg tcggagtcgg tctcctcgtg gcgacgctcg atgttcggga tcacgccctc
     1081 gaacaccgtc cagtacgcgc gctgccgccc gtagcggttc cggtagcgga cgtagatgtc
     1141 cttgtcggag ccgtagagga cgacctctct cgcctcgtcc gggagcttcc gccacggcgt
     1201 cttgagcgag aacccgtgcg cgtcggcgac ggcggcgatc acccggttcc agtactcgag
     1261 ccgcgtcgac gcccacggcg cgatcgcccc ctcggtgatc gccaggtcct tgtccgggac
     1321 gacgagctcg gggtcgacct cgagcctcgt tccgagcccg tcgcacgtct cgcacgcgcc
     1381 atacggcgag ttgaacgaga agttcctggg cgcgagctcg tcgaacgaca gcccgcagtg
     1441 gacgcaggcc aggttctccg agaacgggat gatgtcctcc ttgccgtcgg tctggatcgc
     1501 gacggccgcc atcccctcag ccagctggag cgcggtctcg acggagtccg cgaggcgacg
     1561 ccggatcccc ggcttggcaa cgagccggtc gacgacgacc tcgatcgtgt gcttgtagta
     1621 gcggtcgagg cggatcggct ccgagagctc ccgcacctcg ccgtcgatcc gcgcgcgggc
     1681 gaaccccttc gccgcgaggt ccttgaggag ccgctcgtac tcgcc
//
LOCUS       DASZOB010000442         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_135807, whole genome shotgun sequence.
ACCESSION   DASZOB010000442 DASZOB010000000
VERSION     DASZOB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135807"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VG709_06940"
     CDS             <1..1504
                     /locus_tag="VG709_06940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWC32848.1"
                     /translation="LLSLLARASRRDPFRESERASPADAVARWAAFAVALATFGVSLI
                     VLARFDGADAGFQLVEDATWVEPLNFRYVLGVDGISVFLVLLTTFLMPLAILASWRIE
                     RQVPYFMTALLALETAVLGVFVSLDLLLFFVFFEALLFPMYLVIGMWGYERRVYAAVK
                     FFLFTMAGSAFLLAAILFLYFRAGSVTGTPTFELQQLTDVSASLPAATARWLFLAFFV
                     AFAVKVPLFPLHTWLPDAHTEAPTAGSVILAGVLLKIGAYGLIRFNLTLFPEASRYFR
                     TFVLVLAVIGIVYGAVVALIQSDVKRLVAYSSVSHLGFVVLGIFAGTLEGMSGGVIQM
                     VNHGLSTGALFLLVGMLYERTHTREIARMGGLASQTPALAGFFLVVALSSLALPGLNG
                     FVGEFLVLVGTFVASEPFAVIGAVGVVLSAIYLLWAYQRMMHGPRIDAEPYPDLTVRE
                     YAILVPVVALIVAIGVFPKPFLDRIEPAAERHARVVRVTDDGRPALAEGP"
     gene            1505..2983
                     /locus_tag="VG709_06945"
     CDS             1505..2983
                     /locus_tag="VG709_06945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWC32849.1"
                     /translation="MPQTPEVVFSPILPELIVAGAAIAILLADAIRPIRDQRVLAFLA
                     LVALGAGAWATLRLWGPFAFDPAQPERLTVLGGMVAADRYALFFRLVILGSSAVAVLL
                     ASTYLRRTGEGRGEFFSLLLFATAGMTLLAASADLIMVFLSLEVLSLSLYVMAGFSLR
                     RLASQEASLKYFLLGSFSSAFFLYGVALAYGAAGPPSLAGMAGGVSGGAATGLAIAAA
                     ALLLIGFAFKVAAVPFHMWTPDVYQGAPTPVTAFMAAGTKVAGFAALMRVTQVALGGL
                     GWDLQPVLYAVAIVTMVVGSLVAIAQSDVKRMLAYSSIAHAGFILVGVTAGNQEGISG
                     GMFYLVAYAAMIMGAFGVVMLVSRRGEGRTSLDSYRGLGRTNPGLAGLLALFLLSLAG
                     IPPLAGFWGKVFVFEAAIAAGLWPLVVIGVLASVVAAFFYIQVLVLAYMQDPVEAEAV
                     AEPAAAPAVALIATAAVTVVFGFFPGLILGPLGEAAVLHWVP"
     gene            2980..>3894
                     /locus_tag="VG709_06950"
     CDS             2980..>3894
                     /locus_tag="VG709_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWC32850.1"
                     /translation="MTRAAWTLDAPDPALEADVRRRLDEVEDALGRSARSDVPLVSST
                     STYMLGAGWKRFRPLLVILGGHFGDPHDERLVPGAVAIELTHLATLHHDDVIDEADSR
                     RGIPSVNARWSNTVAILTGDFLFARASEISSDLGTDVTRLLAITIARVCEGQIREADA
                     LGRLEVDESSYMEVIRRKTASLIATSCRLGGMLGDADPRDVDVLERFGEALGLAFQLS
                     DDIMDFTADEETLGKEPAVDLREGVYTLPVIYALERSRRRDELRTLLEQGPPTGERLA
                     GAVRIIRSDGALDLARDAVTREVRRAITE"
BASE COUNT          429 a         1377 c         1360 g          728 t
ORIGIN      
        1 gctgctctcg ctcctcgcgc gcgcgtcgcg ccgagacccc ttccgcgagt ccgagcgggc
       61 gtctccggcc gatgccgtcg cccggtgggc ggcgttcgcc gtcgcgctcg cgacgttcgg
      121 cgtctcgctc atcgtcctcg cccgcttcga tggcgcggat gccggcttcc agctggtcga
      181 agacgcgacg tgggtggagc ccctcaactt ccggtacgtc ctcggggtcg acggcatcag
      241 cgtcttcctc gtgctcctga ccacgttcct gatgccgctc gcgatcctcg cgtcgtggcg
      301 gatcgagcgg caggtgccgt acttcatgac ggcgctgctc gcgctcgaga ccgcggtgct
      361 cggcgtgttc gtctcgctcg atctgctgct gttcttcgtg ttcttcgagg cgctgctgtt
      421 cccgatgtac ctcgtcatcg ggatgtgggg ctacgagagg cgcgtctatg cggccgtgaa
      481 gttcttcctg ttcacgatgg cgggttccgc gttcctgctc gcggcgatcc tgttcctgta
      541 cttccgcgcg gggtccgtga ccgggacgcc gacgttcgag ctccagcagc tcaccgacgt
      601 ctcggcgtcg ctcccggccg cgacggcgcg ctggctcttc ctcgcgttct tcgtcgcgtt
      661 cgccgtgaag gtgccgctgt tccccctgca cacgtggctg ccggacgcgc acaccgaggc
      721 tccgacggct gggtccgtga tcctcgccgg agtcctcttg aagatcgggg cgtacgggct
      781 gatccggttc aacctcacgc tgtttccgga agcgtcgcgg tacttccgga cgttcgtgct
      841 ggtcctcgcc gtgatcggga tcgtctacgg cgccgtggtc gcgctgatcc agagcgacgt
      901 caagcggctc gtcgcgtact cgagcgtctc gcacctcggg ttcgtcgtgc tcgggatctt
      961 cgccgggacg ctcgagggga tgtcgggcgg cgtgatccag atggtgaacc acggcctttc
     1021 caccggcgcg ctgttcctgc tggtcggcat gctctacgag cggacgcaca cgcgcgagat
     1081 cgcacggatg ggagggctcg cctcccagac ccccgcgctc gccgggttct tcctcgtcgt
     1141 cgcgctctcg tccctcgcgc tgccggggct gaacgggttc gtcggtgagt tcctcgtgct
     1201 cgtcggcacg ttcgtggcga gcgagccgtt cgcggtgatc ggcgccgtcg gggtcgtcct
     1261 ctccgcgatc tacctgctgt gggcgtacca gcggatgatg cacggcccca ggatcgacgc
     1321 cgagccctac ccagacctga cggtgcgcga gtacgcgatc ctcgtaccgg tcgtggcgct
     1381 gatcgtcgcg atcggcgtct tcccgaagcc gttcctcgac cgcatcgagc cggcggccga
     1441 acgccacgcg agggtcgtcc gggtcacgga cgacggtcgc ccggctctcg cggagggccc
     1501 gtagatgccc cagacgcccg aggtcgtctt ctcgccgatc ctgccggagc tcatcgtcgc
     1561 gggcgccgcg atcgccatcc tcctcgccga cgcgatccgc cctatccgag accagcgggt
     1621 gctggcgttc ctcgcgctcg tcgcgctcgg ggcgggcgcg tgggcgacgc tccgcctctg
     1681 ggggccgttc gcgttcgatc cggcacagcc cgagcggctg acggtcctcg gcgggatggt
     1741 cgcggcggac cgctacgcgc tgttcttccg cctcgtgatc ctcggctcgt cggcggtcgc
     1801 ggtcctgctt gcgtcgacgt acctccgtcg aaccggcgaa gggcgcggcg agttcttctc
     1861 tctgctcctg ttcgcgaccg cagggatgac gctgctcgct gcgtcggcgg atctcatcat
     1921 ggtgttcctg tctctcgagg tgctctcgct ctcgctgtac gtgatggccg gcttctcgct
     1981 ccggcggctc gcctcccagg aggccagcct caagtacttc ctgctggggt cgttctcgtc
     2041 ggcgttcttc ctctacgggg tcgcgctcgc gtacggtgcg gcgggaccgc cgagcctcgc
     2101 ggggatggca ggaggggtat cgggcggcgc ggcgacgggc ctcgcgatcg ctgccgccgc
     2161 gctgctgctg atcgggttcg cgttcaaggt cgccgcggtc ccgttccaca tgtggacgcc
     2221 cgacgtctac cagggcgcgc cgacgcccgt gacggcgttc atggcggccg ggacgaaggt
     2281 cgccggattc gcggcgctca tgcgggtcac gcaggtcgcg cttggcggcc tcgggtggga
     2341 cctccagccg gtgctctacg ccgtcgcgat cgtgacgatg gtcgtcggct cgctggtcgc
     2401 gatcgcgcag tccgacgtca agcgcatgct cgcgtattcc tcgatcgcgc acgccgggtt
     2461 catcctcgtc ggggtgaccg ccgggaacca ggaggggatc tccggcggga tgttctacct
     2521 cgtcgcgtac gccgcgatga tcatgggggc gttcggtgtc gtgatgctgg tctcccggcg
     2581 cggtgagggc cggacgtccc tcgactcgta ccgcggcctc gggcggacga atccggggct
     2641 cgccgggctc ctcgcgttgt tcctcctctc gctcgccggc atcccgccgc tcgcgggctt
     2701 ctgggggaag gtgttcgtgt tcgaggccgc gatcgcggcc ggactgtggc cgctcgtcgt
     2761 gatcggcgtg ctcgcgagcg tcgtggcggc gttcttctac atccaggtgt tggtgctcgc
     2821 gtacatgcag gatccggtcg aggccgaagc cgtcgcggaa cccgccgccg cgccggccgt
     2881 cgcgctcatt gcgaccgccg ccgtcaccgt cgtcttcggg ttcttccccg ggctcatcct
     2941 cgggccgctc ggagaggcgg cggtcctgca ctgggtgccg tgacgcgggc ggcctggacg
     3001 ctcgacgctc ccgacccggc gctcgaggcc gacgtccgtc gccgcctcga cgaggtggag
     3061 gacgcgctgg gccgctccgc tcgctccgac gtcccgctcg tgagctccac gtcgacgtac
     3121 atgctcggcg ccggctggaa gcggttccgc ccgctgctcg tcatcctcgg cgggcacttc
     3181 ggcgatccgc acgacgagcg actggtgccc ggcgccgtcg cgatcgagct cacgcacctc
     3241 gcgacgctcc accacgacga cgtgatcgac gaggcggact cgcgacgcgg catcccgtcg
     3301 gtcaacgcgc gctggagcaa caccgtggcg atcctgaccg gcgacttcct gttcgcgcgc
     3361 gcctccgaga tctcgtcgga tctcgggacc gacgtcacgc ggctcctcgc gatcacgatc
     3421 gcacgcgtct gcgagggaca gatccgcgag gccgacgcgc tcggccggct cgaggtcgac
     3481 gagtcgtcgt acatggaagt cataagaagg aagaccgcct cgctcatcgc gacgtcgtgc
     3541 cgcctcgggg ggatgctcgg cgatgccgac ccacgggacg tcgacgtcct cgagcggttc
     3601 ggcgaggcgc tcggtctcgc gttccagctc tccgacgaca tcatggactt cacggccgac
     3661 gaggagacgc tcgggaagga gccggcggtc gacctgaggg agggcgtcta cacgctgccg
     3721 gtgatctacg cgctcgagcg ctcgaggcgc cgcgacgagc tgcggacgct tctcgagcag
     3781 ggcccgccga ccggcgagcg gctggctggc gcggtccgga tcattcgctc cgacggcgcg
     3841 ctcgacctcg cccgcgacgc cgtcacgcgg gaggttcggc gcgcgatcac ggag
//
LOCUS       DASZOB010000443         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_176238, whole genome shotgun sequence.
ACCESSION   DASZOB010000443 DASZOB010000000
VERSION     DASZOB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176238"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1003
                     /locus_tag="VG709_06955"
     CDS             101..1003
                     /locus_tag="VG709_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWC32851.1"
                     /translation="MDGFDLDRYLRASKKVDLEGVAWDRVAEHPLPVDEARCLAYMMD
                     IESHTVVFLRDLLATRAALEPDVTAFLACWVYEELWHGEAFSRFLGEAGYQLGPDREE
                     VRGDAPYPSRIGRNAWIRRKLGGKGYAAHVGTMVGSLLVKDFVALHMTWGAGNELSTL
                     SGYQTLTRRTSHPVLIELLRRIIKDERRHFAFYRAQARMRLDRSPRARRVTRWVMERL
                     WAPVGTGVRPQAETDFVVATLFGDADGLAAAREMDQTIAELPGFDGARLIERARGEAI
                     GRSRPRRAGRTERVAAPLAAFRAS"
     gene            complement(1000..2139)
                     /locus_tag="VG709_06960"
     CDS             complement(1000..2139)
                     /locus_tag="VG709_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWC32852.1"
                     /translation="MAPSADKTQWRDLYHEVVATGLCTGCSACVVVCPFHVLGYEDDK
                     PVQLMPDGVDVCTHGDRGCDICTRACPRFREWEGEIDQLLFGRTRLPEEAIGVHRDVV
                     LARATDPVSLAHGQDGGVVSALLVWGLRTGEIDGALTSKLSDERRWDAEPAVVTTPEE
                     VYATAGSRYTYSANPIALTKAAEMGLSKVALVGMSCQASVTGALEARRLNKYRRRIAW
                     TLGLLCSKTFTYDGVMGEIAQERLGLDLDDVVRVNVKGKLLFYTKDGEEHTHSLKDAR
                     EFTRPGCMRCPDFAAEHADISFGGLGQTEGWTLTVIRTDRGEDIFTRALKDGVVEARP
                     GTDDPGAVALMEKLAVKSRKRWPGADVPTADTGPGVLPNGDAAPA"
BASE COUNT          301 a          805 c          819 g          326 t
ORIGIN      
        1 ccggggcctt ccgctgtgtc ggcgcgagct cctgggcggg ccgactcatc caccggatgt
       61 cgttccgcga atcgatggag acatcgaccc cggaggaccc atggacggct tcgacctcga
      121 ccgctacctg cgagcttcca agaaggtgga cctcgagggc gtcgcgtggg accgggtggc
      181 cgagcaccca ctcccggtcg acgaggcgcg ctgcctcgcc tacatgatgg acatcgagtc
      241 gcacacggtc gtgttcctgc gggacctgct cgcgacgcgc gcggcgctgg agcccgacgt
      301 gaccgcgttc ctcgcctgct gggtctacga ggagctctgg cacggcgagg ccttcagccg
      361 cttcctcggc gaggccggct accagctcgg gccggaccgg gaggaggtcc ggggcgacgc
      421 gccgtacccg tcgcggatcg gtcggaacgc gtggatccgg cggaagctcg gcgggaaggg
      481 ctacgccgcg cacgtcggca cgatggtcgg ctcgctcctc gtgaaggact tcgtcgcgct
      541 ccacatgacg tggggtgccg gcaacgagct ctcgacgctc tccggctacc agacgctgac
      601 ccgccggacc tcgcacccgg tcctcatcga gctgctgcga cggatcatca aggacgagcg
      661 ccggcacttc gcgttctacc gggcgcaggc gcgcatgcgt ctcgaccgca gcccgcgcgc
      721 gcggcgcgtc acccgatggg tcatggagcg cctgtgggcg cccgtgggaa caggcgtgcg
      781 tccgcaggcg gagaccgact tcgtcgtcgc gacgctgttc ggcgacgccg acggcctcgc
      841 cgccgcgcgc gagatggacc agacgatcgc tgagctgccg gggttcgatg gtgctcgtct
      901 gatcgaacga gcgcgcgggg aggccatcgg ccgctcgcgg ccccgtcgcg cggggcggac
      961 ggagcgggtc gcggcgccgc tcgccgcgtt ccgcgcctcc taggccggcg cggcgtctcc
     1021 gttcgggagg acgccgggac cggtgtccgc cgtcggcacg tccgcgcccg gccagcgctt
     1081 gcgcgatttc acggcgagct tctccatcag cgcgaccgcc ccggggtcgt cggtgccggg
     1141 ccgcgcctcg acgacgccgt ccttgagcgc gcgcgtgaag atgtcctcgc cgcggtcggt
     1201 gcggatgacc gtgagcgtcc agccctcggt ctgccccaac ccgccgaagg agatgtccgc
     1261 gtgctccgcg gcgaagtcgg ggcagcgcat gcatcccggc cgcgtgaact cgcgcgcgtc
     1321 cttcagcgag tgcgtgtgct cctcgccgtc cttcgtgtag aagagcagct tgcccttgac
     1381 gttgacccgc acgacgtcgt cgaggtcgag ccccagccgc tcctgcgcga tctcgcccat
     1441 cacgccgtcg taggtgaacg tcttggagca cagcagcccg agcgtccagg cgatgcgccg
     1501 gcggtacttg ttcagccgcc tcgcctcgag cgctccggtc acactcgcct gacagctcat
     1561 cccgacgagg gcgaccttcg agaggcccat ctccgccgcc ttcgtcagcg cgatcgggtt
     1621 cgcggagtag gtgtagcgcg aaccggccgt cgcgtagacc tcctccggcg tcgtgacgac
     1681 ggccggctcg gcgtcccacc ggcgctcgtc ggagagcttc gaggtcagcg cgccgtcgat
     1741 ctcgccggtg cgcagccccc agacgaggag ggcggagacg accccgccgt cctggccgtg
     1801 ggccagcgag accgggtcgg tcgcccgggc gaggacgacg tcgcggtgca cgccgatcgc
     1861 ctcctcgggc aggcgcgtgc ggccgaagag cagctggtcg atctcgccct cccactcgcg
     1921 gaagcgcggg caggctcgcg tgcagatgtc gcagccgcgg tcgccgtgcg tgcagacgtc
     1981 gacgccgtcc ggcatcagct ggaccggctt gtcgtcctcg tagccgagga cgtggaaggg
     2041 gcagaccacg acgcaggcgg agcatcccgt gcagaggccg gtcgcgacga cctcgtggta
     2101 gaggtcacgc cactgcgtct tgtccgcgga gggggccacc gccaggcagt atagggtccg
     2161 gcccggcggc gaccggaacg gacgcgggag gtctcggcga tgctagacgg agaacgactc
     2221 gccgcaggcg cactcgtgga cggcccgcgg g
//
LOCUS       DASZOB010000444         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_80165, whole genome shotgun sequence.
ACCESSION   DASZOB010000444 DASZOB010000000
VERSION     DASZOB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80165"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VG709_06965"
     CDS             complement(<1..659)
                     /locus_tag="VG709_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWC32853.1"
                     /translation="MAERARSEMPTAYEPAGVEKRWYGEWIERRYFGADPNPERRPFT
                     IVVPPPNVTGALHMGHALTFTLQDVVIRRARMQGFETLFLPGTDHAGIATQVVVERHL
                     REQGIEDPRSLGRDAFVERVWEWKEQYGNTIVEQMKALGTSCDWERLRFTMDEGLSRA
                     VRVAFVRLYEDGVIYRGERIINWCPTDHTALSDSELEHEEVDGELVTFRYELADGEGH
                     I"
     gene            712..975
                     /locus_tag="VG709_06970"
     CDS             712..975
                     /locus_tag="VG709_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32854.1"
                     /translation="MTRYTDLPLDETDLVTTGGPGEWFEGDVRDPPDVRELRRDAAGA
                     PTNTMVVHTSFEVVKRTASETDLALRDRATPPPRPADGPALRL"
     gene            1016..1324
                     /locus_tag="VG709_06975"
     CDS             1016..1324
                     /locus_tag="VG709_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWC32855.1"
                     /translation="MGIGRKSPRVPGRLESVVRHPGGAACRFAGCERRISLLSHHLRH
                     WADGGATDVDNLVTLCAPHHRYVDEDGWRISGDPHADLRFHDPTPRAPARASLTTSAA
                     "
     gene            complement(1355..>2005)
                     /locus_tag="VG709_06980"
     CDS             complement(1355..>2005)
                     /locus_tag="VG709_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC32856.1"
                     /translation="KILEGTTASVPPQGGRTHPHQEFIQIDTTNVLFICGGSFSGLEQ
                     IIESRVGNRGIGFGADVRKTEDKDLGAILGQVLPEDLLKYGLIPEFVGRLPVITHVHN
                     LDKQALIDILTKPKNALVKQYRKFFDFDGVELAFTDDAMDAIADQAMLRGTGARGLRA
                     IMEEVLLGVMYDLPSRKDVRKCVVDANVVKEGVNPTLVPKAAEAARQSRTRRERSA"
BASE COUNT          302 a          705 c          675 g          323 t
ORIGIN      
        1 tcgatgtgac cctcgccgtc cgcgagctcg tagcggaacg tcacgagctc gccgtcgacc
       61 tcctcatgct cgagctcaga gtcggacagc gccgtgtggt ccgtcgggca ccagttgatg
      121 atgcgttcgc cgcggtagat cactccgtcc tcgtacaagc ggacgaacgc gacgcggacc
      181 gccctcgaca ggccctcgtc catcgtgaac cggagacgtt cccagtcgca ggaggtcccg
      241 agcgccttca tctgctcgac gatcgtgttg ccgtactgct ccttccactc ccacacgcgc
      301 tcgacgaacg cgtcgcgtcc gagcgaccgc gggtcctcga tcccctgctc gcgcaggtgg
      361 cgctccacga cgacctgcgt cgcgatcccc gcgtggtccg tgccggggag gaacagcgtc
      421 tcgaatccct gcatccgcgc acggcggatc acgacgtcct ggagcgtgaa ggtgagggcg
      481 tgccccatgt ggagggcacc cgtgacgttc ggaggcggta cgacgatcgt gaacgggcgg
      541 cgctctgggt tcgggtcggc gccgaagtac cggcgctcga tccactcgcc gtaccagcgc
      601 ttctcgacgc cggcgggctc gtacgccgtc ggcatctcgg aacgggcgcg ctccgccatg
      661 tcgagcagtg taccggcggg cggcgttcag actgtcatgg agaacggtgc tgtgacccga
      721 tacaccgacc tccccctcga cgagaccgac ctcgtcacca caggcggtcc tggagaatgg
      781 ttcgagggcg acgtccgcga cccccccgac gtgcgcgagc tccgtagaga tgccgcgggc
      841 gcgcccacga acacgatggt cgtccacacc tcgttcgagg tggtcaagcg caccgcctcg
      901 gagaccgatc tggctctgcg agaccgagca acgccgccgc ctcgacccgc agacggcccg
      961 gcgcttcggc tgtgacgccg acctcgagtg gatcctcgaa gacgagcggc gccccgtggg
     1021 gatcggcagg aagagcccgc gggtacccgg ccggctcgag tccgtggtgc gccaccccgg
     1081 cggcgcggcc tgccggttcg ccggatgcga gcggcggatc tccttgctct cgcaccacct
     1141 ccggcactgg gccgacggtg gagccaccga cgtcgacaat ctcgtgacgc tctgcgcccc
     1201 gcaccaccgg tacgtcgacg aggacgggtg gcggatctcc ggcgatccgc acgcggacct
     1261 ccggttccac gaccccacac cacgggcgcc agcgcgggcg tcgctgacga catcggcagc
     1321 ctgacggttc tacggctgga gcgagaagcg cgaactacgc cgagcgctcg cggcgggtac
     1381 ggctctgccg cgcggcctcg gcggccttcg ggacgagcgt cgggttgacg ccctccttga
     1441 cgacgttggc gtcgacgacg cacttgcgga cgtccttgcg cgacgggagg tcgtacatca
     1501 cgccgaggag aacctcctcc atgatcgcgc ggagcccgcg cgcgccggtc ccgcggagca
     1561 tcgcctggtc ggcgatcgcg tccatcgcgt cgtcggtgaa cgccagctcg acgccgtcga
     1621 agtcgaagaa cttccggtac tgcttgacca gcgcgttctt cggcttggtg aggatgtcga
     1681 tgagcgcctg cttgtcgagg ttgtgcacgt gggtgatcac gggaagccgg ccgacgaact
     1741 ccgggatcag cccgtacttc aggaggtcct cggggagaac ctgtccgagg atcgcgccga
     1801 ggtccttgtc ctcggtcttt cgcacgtcgg cgccgaaccc gatcccgcgg ttcccgaccc
     1861 tggactcgat gatctgctcg agccctgaga acgatccgcc gcagatgaac aggacattcg
     1921 tggtgtcgat ctggatgaac tcctggtgcg ggtgcgtgcg cccgccctgg ggcggaacgc
     1981 tggccgtcgt cccctcgagg atctt
//
LOCUS       DASZOB010000445         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_100699, whole genome shotgun sequence.
ACCESSION   DASZOB010000445 DASZOB010000000
VERSION     DASZOB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100699"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VG709_06985"
     CDS             <1..342
                     /locus_tag="VG709_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HWC32857.1"
                     /translation="GEYRVFNQFTESFTLLELAELVRKAGAEYGIEVRIDHLENPRTE
                     KEEHYYNAKHTKLLDLGLQPHYLSETLIESMFATIERYRDRVIRDHILPSITWRAGAR
                     SRTPEPVAAER"
     gene            362..1408
                     /locus_tag="VG709_06990"
     CDS             362..1408
                     /locus_tag="VG709_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HWC32858.1"
                     /translation="MLALRIVGAIVTFALFAVVVARYRGRKVSRLNLILAGTFTLVVI
                     ALAARPTMFNPLFDLFRIQAGGGRRMMAALLVGMFFLLVAVIRALGQADVNERTVRML
                     IESLAVDSFDWSQARELPDGERIVVAMPAHNEADNIAPVIHSMPDQVEGLPVVTVVVD
                     DQSDDDTAGVAARAGALVARLPIRRGQGMAQRVGYEIGLKLGAVVVVTMDSDGQHVPE
                     EMGLLVKPILEDQADMVQGSRILGEFAKESHLRHAGVMFFSRLVSLMTGTRITDFSSG
                     YRAIRADILKHLVLEQDQFSSSEVLVEGLRQHARIREVPITIRARSSGLSKKPKNLRY
                     GWSFTKVIVKTWLR"
BASE COUNT          233 a          534 c          525 g          238 t
ORIGIN      
        1 ggcgagtacc gcgtgttcaa ccagttcacg gagtccttca cgctgctgga gctcgccgaa
       61 ctcgtccgga aagcgggcgc ggagtacggc atcgaggtgc ggatcgacca cctcgagaac
      121 ccccgcacgg agaaggaaga gcactactac aacgccaagc acacgaagct gctggatctc
      181 gggctccagc cccactacct ctcggagacg ctgatcgaat cgatgttcgc gaccatcgag
      241 cgctaccggg accgcgtcat ccgcgaccac atcctgccca gcatcacgtg gcgggcgggc
      301 gcgcggtcga ggacgccgga gcccgtggcc gcggaacgct aggagctcgg gaggcccggc
      361 gatgctcgcg ctgcggatcg tcggagccat cgtcaccttc gccctgttcg cggtcgtcgt
      421 cgcgcgctac cgcggccgga aggtctctcg cctgaacctg atcctcgcgg ggacgttcac
      481 gctcgtcgtg atcgcgctcg cggcgcgacc gacgatgttc aacccgctct tcgacctctt
      541 ccggatccag gccggtggcg ggcgtcggat gatggcggcg ctcctcgtcg ggatgttctt
      601 cctcctggtc gccgtgatcc gcgcgctcgg gcaggccgac gtgaacgagc gcacggtgcg
      661 gatgctgatc gagtcgctcg cggtcgactc cttcgactgg tcgcaggcga gggagctgcc
      721 cgacggcgaa cgcatcgtcg tcgcgatgcc ggcgcacaac gaggcggaca acatcgcgcc
      781 ggtgatccac tcgatgcccg atcaggtgga ggggctgccg gtggtcaccg tcgtcgtgga
      841 cgaccagtcg gacgacgaca ccgcgggcgt cgcggcgcgg gccggcgcgc tcgtcgcccg
      901 cctgccgatc cggcgtggac agggcatggc ccaacgggtc ggctacgaga tcgggctgaa
      961 gctcggcgcc gtggtcgtcg tgacgatgga ctccgacggc cagcacgtcc ccgaggagat
     1021 gggtctcctc gtcaagccga tcctcgagga ccaggccgac atggtccagg gctcgcggat
     1081 cctcggcgag ttcgcgaagg agagccacct gcgccacgcc ggcgtgatgt tcttctcgcg
     1141 cctcgtctcg ctcatgacgg gcacccggat caccgacttc tcgagcgggt accgcgcgat
     1201 ccgcgccgac atcctcaagc acctcgtcct cgagcaggac cagttctcct cctcggaggt
     1261 cctggtcgag gggctgcggc agcacgcccg gatcagggag gtcccgatca cgatccgcgc
     1321 gcggtcgagc ggactgtcga agaagcccaa gaacctccgg tacggctgga gcttcacgaa
     1381 ggtcatcgtc aagacctggc tccggtaggc gccgtgtgag ccacgggtga acccggccgg
     1441 cctcgcgctc tcgctcgtcg ccgtggtgct accgggcgcg ggcgcggcgc tcgcgctcca
     1501 tccgcccggc gccgtcgatc tcgcgacgcg
//
LOCUS       DASZOB010000446         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_80196, whole genome shotgun sequence.
ACCESSION   DASZOB010000446 DASZOB010000000
VERSION     DASZOB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80196"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VG709_06995"
     CDS             <1..454
                     /locus_tag="VG709_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC32859.1"
                     /translation="IEDADAVRTALRRADVADLESSVPQGLETPLGRAFLEGIELSGG
                     QWQKVALARAMMRTAPLMLVLDEPTYSLDVESERRVFDWFSRIADAENPAGTITVIVS
                     HRFSTVRSADLVAVLHEGRLVELGTHDELMAANGHYAAMYRTQAEGYA"
     gene            complement(461..1645)
                     /locus_tag="VG709_07000"
     CDS             complement(461..1645)
                     /locus_tag="VG709_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32860.1"
                     /translation="MAEVGFRDVTKVFRDGTVAVSSFDLDVPDRTFCVLVGPSGSGKT
                     TLLRMVAGLEEPTEGSIWIGGRDVTDDAPKHRDVAMVFQNFAIYPHMSVLENIAFGLR
                     MRGIKRREIGERVVEAARLLGLEDVLKKRPRHLSTGQLQRVAMGRAIVRRPALFLMDE
                     PLSNLDARLREQMRAEIVRIHRTVAATTLYVTHDQAEAMTLGDLVAVIKDGELQQADT
                     PEEIYERPTNVFVAAFVGSPAMNLVEATLERGDGLWGRFGRHRIRLDDGGDVSALRRY
                     EDRQVILGIRPEHLDDASLVSRDVPPGTTIRTVVARHELIGPEVLLRFNVDAPLLMLR
                     DPRASDEDPDAGMWAVERPNMFVARVDAGTRARDGADVELAVRTDRIHLFDPATGLAI
                     GD"
     gene            1704..>2372
                     /locus_tag="VG709_07005"
     CDS             1704..>2372
                     /locus_tag="VG709_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HWC32861.1"
                     /translation="MRGHDHRDHDTGAPLMPFDEARSLALARIEALAPHDVELREALG
                     FVAAEAIRAPHDLPPFTSSAMDGFAVRARDVDVASRDRPAALRLAGRVEMGAAAEVSV
                     GAGEAVAIPTGAVVPEGADAVVPIERCIVKGDRVLMLEASAPGRFGRPAGQDVRAGEV
                     LVEPGRRLGPPEVGLLASAGIPTVRVHPKARVAIVSTGDELVEVGRTLGPGQVHDANA
                     ATLHA"
BASE COUNT          334 a          866 c          823 g          349 t
ORIGIN      
        1 catcgaggac gccgacgccg tccggaccgc gctccgacgc gccgatgtcg cggatctcga
       61 gtcgtcggtc ccgcaaggcc tggagacgcc gctggggcgc gccttcctcg agggcatcga
      121 gctctcgggc ggccagtggc agaaggtcgc gctggcgcga gcgatgatgc ggaccgcgcc
      181 gctgatgctc gtgctcgacg agccgaccta ctcgctcgac gtcgagtccg agcggcgggt
      241 cttcgactgg ttctcgcgga tcgcggacgc ggagaacccg gcggggacca tcacggtgat
      301 cgtgtcgcac cggttctcga ccgtgcgttc cgccgacctc gtcgccgtcc tgcacgaggg
      361 ccgtctggtg gagctcggga cgcacgacga gctgatggcc gcgaacggtc actacgcggc
      421 catgtatcgc acgcaggcgg agggctacgc ctaacgcgac ctagtcgccg atcgcgagcc
      481 ccgtcgcggg gtcgaagaga tgaatgcgat ccgtccggac cgcgagctcg acgtcggccc
      541 cgtcgcgagc tcgggtcccc gcgtcgaccc gagcgacgaa catgttcggg cgctccaccg
      601 cccacatccc cgcgtcggga tcctcgtcgg acgcccgggg gtcgcgaagc atcaggagcg
      661 gcgcgtcgac gttgaagcgc aggagcacct ccggcccgat cagctcgtgg cgcgcgacga
      721 cggtgcggat cgtcgtcccc ggcggcacgt cgcgcgagac gagcgacgcg tcgtcgaggt
      781 gttccgggcg gatccctaga atcacctggc ggtcctcgta ccgccggagc gccgagacgt
      841 cgccgccgtc gtcgagccgt atccggtggc ggccgaagcg cccccagagc ccgtcgccgc
      901 gctcgagcgt cgcttccacg agattcatcg ccggcgaacc gacgaacgcc gccacgaaca
      961 cgttcgtcgg ccgttcgtag atctcctcgg gcgtatcggc ctgctggagc tcgccgtcct
     1021 tgatcaccgc cacgaggtcg ccgagggtca tcgcctcggc ctggtcgtgc gtcacgtaca
     1081 acgtcgtcgc cgcgacggtt cggtggatcc tgacgatctc ggcgcgcatc tgctcccgga
     1141 gtcgcgcgtc gaggttcgag agtggctcgt ccatcaggaa gagggctggg cggcgaacga
     1201 tcgccctccc catcgccacg cgttgcagct gtcccgtcga caggtggcgc ggccgcttct
     1261 tcagcacgtc ctcgagaccg agcaggcgcg ccgcctcgac gacgcgctcg ccgatctcgc
     1321 gccgcttgat gccgcgcatc cggagcccga acgcgatgtt ctccagcacg ctcatgtgcg
     1381 gatagatcgc gaagttctgg aacaccatcg ccacgtcgcg atgcttgggc gcgtcgtcgg
     1441 tgacgtcgcg gccgccgatc cagatgcttc cctccgtggg ctcctccagg cccgcgacca
     1501 tccggagcag cgtcgtcttg ccggacccgg acggaccgac gagcacgcag aaggtgcggt
     1561 cggggacgtc caggtcgaac gacgagaccg ccacggtccc gtcgcggaag acctttgtga
     1621 cgtctcggaa gccgacctcg gccacgtttc ctccgtgttc gctcgacccg agcagccgac
     1681 gctcgctata cgaataatga cgcgtgcgcg ggcacgacca ccgcgaccac gacactgggg
     1741 cgccgctgat gccgttcgac gaggcccgca gtctcgcgct cgcgcggatc gaggcgctag
     1801 cgccgcacga cgtcgagctc cgagaagcgc tcggcttcgt ggccgcggaa gcgatccgcg
     1861 cgccgcacga ccttccaccg ttcacatcgt cggcgatgga tggcttcgcg gtgcgcgccc
     1921 gcgacgtcga tgtggccagc cgcgaccgcc cggcggcgct gcgcctcgcg ggccgcgtcg
     1981 agatgggcgc cgccgcggag gtctccgtag gcgcgggcga ggccgtcgcg atacccaccg
     2041 gcgccgtcgt tcccgaaggc gccgacgccg tcgtgccgat cgagcggtgc atcgtgaaag
     2101 gcgaccgcgt cctcatgctc gaggcctcgg cgcccggacg gttcggccgt cccgcgggac
     2161 aggacgtccg cgccggcgag gtgctcgtcg agcccggacg ccgcctcgga cctcccgagg
     2221 tcgggctcct cgcgtcggcc ggcatcccga ccgtccgggt gcatccgaag gcccgcgtcg
     2281 cgatcgtctc gaccggcgac gagctcgtcg aggtcggtcg gacgctcgga cccggccagg
     2341 tccacgacgc taacgctgcc accctgcacg cg
//
LOCUS       DASZOB010000447         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_100744, whole genome shotgun sequence.
ACCESSION   DASZOB010000447 DASZOB010000000
VERSION     DASZOB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100744"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VG709_07010"
     CDS             <1..515
                     /locus_tag="VG709_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWC32862.1"
                     /translation="KTTSKKPGSKKATTRTAGPAARAATTPPRARIQAPPPRPERRVR
                     PAKFDPKQLAAIREQLAKERVELEQQLTEVEEESFTGTQSELTGDAGLNDDFADAGSA
                     TFDRENALSIRNNIRDLIDQITRAMGRIDEGTYGTCERCGKPIDAARLKALPHALLCM
                     DCKRREERAR"
     gene            612..1142
                     /gene="lspA"
                     /locus_tag="VG709_07015"
     CDS             612..1142
                     /gene="lspA"
                     /locus_tag="VG709_07015"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWC32863.1"
                     /translation="MSDRPLPTGVYPRLLGAAAVVLALDQITKTLALEALSDEPIDII
                     EGAVTLELSYNSGGVFGVGRGFPGVFLAATIIVTLGILLWARRIEERTWLVPLGMVLG
                     GGLGNLADRVFRGHDGQVVDFIDLHVWPVFTLADTSIVLGVGLVLLGALRSDRGEQGE
                     RDVAQAGAAERDDAAP"
     gene            1139..2065
                     /locus_tag="VG709_07020"
     CDS             1139..2065
                     /locus_tag="VG709_07020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWC32864.1"
                     /translation="MTSLFDVSFVPEEAEVGERIDLVLARRTGVTRALARRALESGSV
                     LVARERVKPSHRLVAGEKVEGRIEVEPEALPEAEDIPVDIRYEDEHVLVVSKPAGLVV
                     HPVRSQTGGTLVNALMGLGKPLGMLDPKRPGIVHRLDKDTSGLVLVAKDDATQLRLRE
                     DLKARRIERRYYALVRGVPPGSGTVEAPIGRHATRRTLMAVVSGGRPAITHYEVLQST
                     DQLALLDVKLETGRTHQIRVHLSHIGHPVLGDRVYGGVGELSRSLGLDRPFLHAYRLV
                     FPRGESKRIDVSDPLPDPLRELLERVPLPTAT"
BASE COUNT          355 a          769 c          779 g          344 t
ORIGIN      
        1 ccaagacgac gtccaagaag ccggggagca agaaggcgac cacccgcacc gccggtccgg
       61 ccgcgcgcgc cgccacgaca ccgccgcggg cccggatcca ggcgcctccg cctcgacccg
      121 agcggcgggt gcgcccggcg aagttcgacc ccaagcagct cgccgcgatc cgcgagcagc
      181 tggcgaagga gcgggtcgag ctcgagcaac aactaaccga ggtcgaggag gagtccttca
      241 ccggcaccca gagtgagctg acgggcgatg cggggctcaa cgacgatttt gcggacgcgg
      301 gctcggcgac gttcgatcgc gagaacgcgc tgtcgatccg caacaacatc agggacctga
      361 tcgaccagat cacccgcgcg atggggcgga tcgatgaggg cacctatggg acctgtgagc
      421 gctgcggcaa acccattgac gcggcgcgcc tgaaggcctt gccccacgcg ctcttgtgca
      481 tggactgcaa gcggcgggag gagcgggcgc gctgagcctc agatccgatg tctcttcctc
      541 cggaggaagc cgcctctagt cagtcgcgcg agcacacggc cgagcacgca gccgcctctt
      601 ccgggacaac gttgtccgat cgcccactgc caaccggcgt ctacccgcgc ctcctcggcg
      661 cggccgcggt cgtcctcgcg ctcgaccaga tcaccaagac gctcgcgctg gaagcgctgt
      721 ccgacgagcc gatcgacatc atcgagggag ccgtgacgct cgagctgtcc tacaactcgg
      781 gcggcgtctt cggcgtcggc cgcggtttcc cgggggtgtt tttggcggcg acgatcatcg
      841 tgacgctcgg catcctgctg tgggcccggc gcatcgagga gcggacctgg ctcgtgccgc
      901 tcgggatggt gctcggcggc gggctcggca acctcgccga tcgcgtcttc cgggggcacg
      961 acgggcaggt cgtggacttc atcgacctgc acgtgtggcc ggtgttcacc ctcgccgaca
     1021 cgtcgatcgt gctcggcgtc ggactcgtcc tgctcggcgc gctgcgttcg gatcgaggcg
     1081 agcaaggcga gcgcgacgtg gcgcaagccg gcgcggcgga acgcgacgac gccgcgccgt
     1141 gacctctttg ttcgacgtct ccttcgttcc cgaggaggcc gaggtcgggg agcggatcga
     1201 cctcgtgctc gcgcgccgga ccggcgtcac gcgcgcgctc gcgcgccggg ccctcgaatc
     1261 gggatccgtg ctggtggcgc gagaacgtgt gaaaccaagc caccgcctcg tcgcgggaga
     1321 gaaggtcgag gggcggatcg aggtcgagcc ggaggcgctt ccggaggccg aggacatccc
     1381 tgtcgacatc cgctacgagg acgagcacgt tctcgtcgtg tccaagcccg ccggcctcgt
     1441 cgttcacccc gtacggagcc agacgggcgg cacgctcgtc aacgcgctga tgggcttggg
     1501 caagcctctc gggatgctcg atccgaagcg gccgggcatc gtgcaccgcc tggacaagga
     1561 cacctcgggc ctggtgctcg tggcgaagga cgacgctacg caactgcggc tgcgcgagga
     1621 cttaaaggcg cgccggatcg agcgtcgcta ctacgctctc gtgcgcgggg taccgcccgg
     1681 ctccgggacc gtcgaggcgc ccatcggtcg ccacgcgaca cggcggacgt tgatggcggt
     1741 ggtgtccggg ggccgacccg cgatcaccca ttacgaggtg ctgcagtcga ccgaccagct
     1801 cgcgctactc gacgtcaagc tcgagaccgg acgcacgcac cagatccggg tgcacctgtc
     1861 gcacatcggc cacccggtgc tgggagaccg cgtctacgga ggagtgggcg agctctcgcg
     1921 ctcgctcggt ctcgaccgcc ccttcctgca cgcctacagg ctggtcttcc cgcgcggaga
     1981 aagcaagcgc atagacgtct cggacccgct tcccgaccct cttcgggaac tcctcgagcg
     2041 cgtcccgctt ccgactgcca cgtaggaccc gtggggaggg attcgcagtt agacgcgggc
     2101 cttgatcagc gaggtcgtcg ggcgctcctc ctttatcggg attgtttctt tgcgcgatag
     2161 ggacctctat acgtgggctg cggcgcaaag acggaatggg tctcgttgtc gaagcgctcg
     2221 aggccctgag gccggcactt caatcac
//
LOCUS       DASZOB010000448         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_60278, whole genome shotgun sequence.
ACCESSION   DASZOB010000448 DASZOB010000000
VERSION     DASZOB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60278"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1639
                     /gene="glmU"
                     /locus_tag="VG709_07025"
     CDS             263..1639
                     /gene="glmU"
                     /locus_tag="VG709_07025"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWC32865.1"
                     /translation="MSERFTVLVMAAGRGTRMRSSLPKVLHPVCGKSMVQWVIDAARG
                     AGASDVVCITRPGDGVAEGLPDDVAVAEQREGEGTGAAVRAAREVVREEGTVVVLSGD
                     HPLVSSELIEALVATHRREQAAATILTTADLDPTGYGRVVRDAGGSVDRIVETKHTEG
                     LSPDLLAIREINLGTYAFEAGELWAALGEVDEDRGERYLTGVFPLIAARGGRVVSHLT
                     RDVTSAVGVNDRVGLMDADRLAQRRILEHHARAGVSFLAPDSIRVEAGVEIGEDTTVG
                     PGVTLRGPTRVGAGCAIGPESTITACTLGADVTVRHSFLVEAIVENGASIGPFAYLRP
                     GAHIGERARIGTFVEVKNSDVGANAKVPHLSYLGDADVGEGANVAAGNITANYDGFAK
                     HRTTIGKGAKTGVNASYVAPVSVGQGAYIAAGSVITEDVPDGALGISRPDQKNVEGYA
                     ERVEKERG"
     gene            1729..2688
                     /locus_tag="VG709_07030"
     CDS             1729..2688
                     /locus_tag="VG709_07030"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWC32866.1"
                     /translation="MVAAGRSSVDLGAKIAERLGVGLTDAGLKTFSDGEVYCRYADSI
                     RGADLFIVQSTCGAEEVGLTVNDALMELMLMVHAAKHASAHRIIAVTPWYGYSRQDKK
                     SAPREPISARLIAELLETAGIARLVAMDLHAGQMQGFFSRPVDHMTAMPILTQFVKDQ
                     LGTDELVIIAPDAGRVKLVRKFAQKVDRPYALLEKERPEHQVAETGYVIGDVEGQIAV
                     IVDDIIDTGGTLRAAAETVMAEGAREVYAVATHGVFSGRAFETLADSPLSGIVVTDTV
                     PLRDGAPEMIRVLTTADILTDTVRKIFTDDSVSEIFQGENQLF"
     gene            complement(2710..>3262)
                     /locus_tag="VG709_07035"
     CDS             complement(2710..>3262)
                     /locus_tag="VG709_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWC32867.1"
                     /translation="AHPGNYILMDDGKLAFLDFGMTKQLDKEQIELEIAALEAVFAGD
                     PERLRVAMHDLGFLKNPRKVDAEKLMAHVMTVGGWYMEDREITIDSRLVMEAISAVSD
                     PRSEFFDLMRRENVPANELMGRRMEVGVMAVLGQLQATRNWYAIGREWWFCDEPGTEL
                     GRQERAYFESRGERTVRRFSARA"
BASE COUNT          516 a         1147 c         1113 g          486 t
ORIGIN      
        1 caaatgggtc cggtggtatc gatacgggcg caagtcgcga tccgaagacg ctgactaacc
       61 cgccagcttt aaggacccca tgttcgacca catctccctc gcggtcgccg cctccccgcg
      121 agcccgtgtt ccccggagct cgctgccgac actacgacgc gctgctcgat cccgacggca
      181 acgatgtcga gggcggtctg cgggccggcc gggctgagct ccccgcacac atcgctgcgc
      241 tcgcaggtac cctgcccgtc ccatgtccga gcggttcacc gtgctcgtga tggccgccgg
      301 gcgcggcacc cgcatgcggt cctcgcttcc gaaggtgctg caccccgtct gcggcaagtc
      361 gatggtccag tgggtgatcg acgcggctcg gggcgccggt gcatccgacg tcgtctgcat
      421 cacccgcccg ggcgacgggg tggccgaggg gctgccggac gacgtcgcgg tggccgagca
      481 gcgcgagggc gaaggcaccg gcgcggccgt tcgggcggcg cgcgaggtag tccgcgagga
      541 gggcacggtg gtcgtcctct ccggtgacca cccgctcgtc tcatccgagc tgatcgaggc
      601 gctcgtggcg acccatcgcc gcgagcaggc ggcggccacc atcctcacga ccgccgacct
      661 cgaccccacg gggtacggcc gcgtcgtgcg ggacgcgggc ggctcggtgg atcgcatcgt
      721 cgagacgaag cacaccgagg gtctcagccc cgacctgctg gcgatccggg agatcaacct
      781 cgggacctac gccttcgagg ccggcgagct ctgggccgcg ctcggcgagg tcgacgagga
      841 ccgcggcgag cgctacctca ccggcgtctt cccgctgatc gctgcacgcg gagggcgagt
      901 cgtctcccat ctcacgcgcg acgtcacgag cgcggtcggc gtgaacgatc gcgtcggcct
      961 gatggacgcc gaccgcctcg cgcaaagacg gatcctcgag caccacgcgc gggccggcgt
     1021 gagcttcctc gcccccgact cgatccgcgt cgaggccggc gtcgagatcg gcgaggacac
     1081 cacggtcggg cccggcgtga cgctccgcgg cccgacacgg gtcggagcgg gctgtgcgat
     1141 cggcccggag tcgaccatca ccgcctgcac gctgggcgcg gatgtcaccg ttcgtcactc
     1201 gttcctcgtc gaggcgatcg tcgagaacgg ggcgtcgatc gggccctttg cctacctgcg
     1261 cccgggcgct cacatcggcg agcgagccag gatcggaacc ttcgtcgagg tcaagaactc
     1321 cgatgtcggc gcgaacgcca aggtgcctca cctgtcctac ctcggtgacg ccgacgtggg
     1381 cgaaggcgcc aacgtggccg cgggcaacat caccgccaac tacgacggct tcgccaagca
     1441 ccgcacgacg atcggcaagg gcgcgaagac cggcgtcaac gcgtcgtatg tggcgcccgt
     1501 gagcgtcggg cagggcgcgt atattgcagc cggatccgtg atcaccgagg acgtcccgga
     1561 cggagcgctc ggaatctcgc gacccgatca gaagaacgtc gagggatacg cagagcgcgt
     1621 cgagaaggaa cgcggttgac cagcatggac tcgcagaacg gagatctggg gcgcaccgag
     1681 tcgccggtgc tcgagacgtt catcccggcc gggtacgaca agcgcctgat ggtcgcggcg
     1741 gggcgctcga gcgtcgacct gggcgccaag atcgccgagc gcctgggcgt cgggctcacc
     1801 gacgccggac tcaagacctt ctcggacggc gaggtctact gccgctacgc cgactccatc
     1861 cgcggtgccg acctcttcat cgttcagtcc acctgcggtg ccgaggaggt gggacttacc
     1921 gtgaacgacg cgctgatgga gctgatgctc atggtgcacg cggccaagca cgcctcggcg
     1981 caccgcatca tcgcggtcac cccgtggtac ggctactcgc gccaggacaa gaagtcggct
     2041 ccgcgcgagc cgatctcggc gcggctgatc gcagagctgc tcgagaccgc gggcatcgcc
     2101 cgtctcgtgg cgatggatct ccatgccggt cagatgcagg gcttcttctc gaggccggtc
     2161 gaccacatga cggcgatgcc gattctcacc cagttcgtca aggaccagct gggcaccgac
     2221 gagctcgtca tcatcgcccc cgacgcgggg cgcgtgaagc tcgtgcgcaa gttcgcgcag
     2281 aaggtcgatc gtccgtacgc cctgctcgag aaggagcggc ccgagcacca ggtcgcggag
     2341 accggctacg tgatcggcga cgtcgagggg cagatcgcgg tgatcgtgga cgacatcatc
     2401 gacaccggcg gaacgctcag ggcggcggcg gagacagtga tggccgaggg cgccagggag
     2461 gtctacgcgg tggccaccca cggcgtcttc tcggggcggg cgttcgagac gctcgccgac
     2521 tccccgctct cgggcatcgt cgtgacggac accgtcccgc tgcgcgacgg cgcccccgag
     2581 atgatccgcg tgctgacgac ggccgacatc ctcacggaca ccgtccgcaa gatcttcacc
     2641 gacgactcgg tctcggagat cttccagggc gagaaccagc tgttctagcc gccgggcggg
     2701 cgggtacggt caggcgcgag cggagaacct ccgcacggtg cgctcgccgc gcgactcgaa
     2761 gtacgctcgc tcctgccgtc ccagctcggt gcccggctcg tcgcagaacc accactcgcg
     2821 gccgatcgcg taccagttgc gggtcgcctg cagctgcccg aggacggcca tgacgccgac
     2881 ctccatgcgc cgacccatca gctcattggc gggcacgttc tctcgccgca tcaggtcgaa
     2941 gaactcggag cgcgggtccg agaccgccga gatcgcctcc atcacgagcc gcgagtcgat
     3001 cgtgatctcg cgatcctcca tgtaccagcc gccgaccgtc atcacgtgcg ccatgagctt
     3061 ctcggcgtcg accttccgcg ggttcttgag aaagccgagg tcgtgcatcg ccacgcgcag
     3121 gcgctcgggg tccccggcga agaccgcctc cagcgccgcg atctccagct cgatctgctc
     3181 cttgtcgagc tgcttcgtca tcccgaagtc gaggaatgcg agcttcccgt cgtccatcag
     3241 gatgtagttg ccggggtggg cg
//
LOCUS       DASZOB010000449         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_120051, whole genome shotgun sequence.
ACCESSION   DASZOB010000449 DASZOB010000000
VERSION     DASZOB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120051"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..2606
                     /gene="alaS"
                     /locus_tag="VG709_07040"
                     /pseudo
     CDS             70..2606
                     /gene="alaS"
                     /locus_tag="VG709_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
BASE COUNT          393 a          894 c          959 g          360 t
ORIGIN      
        1 gcagcagtgc atgcgtctcc tcggcgttcg cgcgacctga gccgacagcg ccccgataca
       61 ctggccgcga tgtacggcag cgaaattcgc cggcgcttca cgcgcttctt cgaggagcgc
      121 gatcacgtcg tcgttccggc gtcgtcgctg attccggacg acccgagcct gctgctcacg
      181 acggccggga tggtccagtt caagccctac ttcctcggcc agcaggcgcc cccgtaccgg
      241 cgggccacat ccgtgcagaa gtcgttccgg acgaccgaca tcgacctcgt cgggcacacg
      301 gaccgtcacc tcacgttctt cgagatgctc ggcaacttct cgttcggcga gtacttcaag
      361 gcgggcgcga tccggttcgc ctgggacctc gtcacccagg ggtacgggat cgacgccggc
      421 cggctctggg cgaccgtgca cgtcgaggac gacgaggcgg ccgaggcgtg gacgacgttc
      481 ctccctccgg agcgcatcgt ccgtcgggac aagccgttcg cggaggcgac gccggaggag
      541 cgcgggaact tcgacaactt ctggtcgatg ggcgtcgccg gaccgtgcgg cccgtgctcg
      601 gagatctaca tcgatcgggg cccccgctac ggacgcgaag gcggcccgga ggtcgacgag
      661 gagcggttcc tctagatctg gaacctcgtg ttcatgcagt acgagcgcga cgacgacttc
      721 aacatcgtcg gagacctgcc caaccagaac atcgacaccg ggagcggcct cgagcgcgtc
      781 gcggtcgttc tccaggatgc ggcgtccgcg ttcgacacag acctgatgcg cccgatcgtc
      841 gaggtcgccg agtcgctgtc cggccggacc tacgggaagg acgagcgcga cgacgtctcg
      901 ctccggatcc tgggcgagca cggtcgcgcc acgacgttcc tcatcgcgga cggcgtgctg
      961 ccgtcgaacg agggccgcgg ctacgtcctt cggcggatgc tgagacgcct cgtctcgcac
     1021 gcccggcgcc tcggcgtcga ggcggaggtc acggggccgc tcgcggaacg cacgatcgag
     1081 ctgatgggcg acgcgtaccc ggacctcttc gagcggaggg cgttcatcca ccaggtcgcc
     1141 gcgtcggagg aggtgcattt cggcgcgacg ctccggcagg ggatgacgct gttcggcgac
     1201 gaggtcgagc gcctgaaggc cggcggggag cgccgtctgc cgggcggggt cgccttccgg
     1261 ctccacgaca cctacggctt caacgtggaa ctcgtcacgg agctcgcgca ggagcagggc
     1321 ctcgaggtcg acgccgagac gttcgagaag ctgatgagcg agcagcggga acgagcgagg
     1381 cgagcggctc acggcggccg cgacgagcgc ggcctcgggg aggccgcgcg gcgcgccggc
     1441 gagaccgact tcctcggata ccagctgctc gagtccgatg cccgcgtggc cgccctcgtc
     1501 gcgggaggcg atctcgtcga gacggcgggc gaaggcgccg aggtcgacgt catcctggac
     1561 cgcaccccct tctacgccga gggcggcgga cagatcggcg acgcgggaac gatcgagacc
     1621 gccgacggac ggctcgatgt gctcgacacc gtcaagggac ccgcgggaac gacggtccat
     1681 cgcgcgcggg tgacgagcgg cgaggcgcac accggcgccg acgtccgggc cgccgtggat
     1741 cccgcgcgtc gcgccgcgac ggcgcgctcg cataccggca cgcacgtcgt ccattggacg
     1801 ctccggaacc tcgtcggcga gcacgcgcgc caggcggggt cgctcgtcgc gcccgggcgg
     1861 ctgcgcttcg acttcacgca cttcgaggcg ctctcgcgcg acctcctcga cgaggtcgag
     1921 gggacggcaa acgcgcggct cgcgcaggac tcgccggtcc gcgcgtacga gacgacgtgg
     1981 gagttcgcgc ggagccaggg ggcgatcgcg ctgttcgagg agacgtacgg cgacatcgtg
     2041 cgcgttgtcg agatcggcga ctactcggtc gagctctgcg gcggtacgca cgtcgcgcgc
     2101 accggcgagg tccagctcgc gctcgtcgta tcggaggggt cgatcggggc aggactccgt
     2161 cgcgtcgagg cgctcgtggg cccggacgcg ctcgcgtgga tcggtaccga gcgccgcctc
     2221 ctccacgaga tcaccgaggc gctcggggcg cgggatccat cccaggcggc ggagcgtgcg
     2281 cgtcgcgcgg tcgcacgggt gaaggagctc gagaaccagc tggggatgct gaaggcccag
     2341 gaccgctcga agcgcgtgac ggacctggtc gcgggcgccc gtgacgtcgc tggcgtgtcg
     2401 ctcgtcgtcg cagagatccg cgacgaggac gccggcgggc tccgcgagat cgctcagcaa
     2461 gtccgaggcc tcctcgagcg cgatggagac ggcgccgcgg tcctcggcac ggcgaacggc
     2521 agcaaggcga cgctcgtggc ggcctgcacg aagcagctcg tcgatcgcgg cgtgacggcg
     2581 tcgagcctcc tcgagccgac cgccct
//
LOCUS       DASZOB010000450         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_151001, whole genome shotgun sequence.
ACCESSION   DASZOB010000450 DASZOB010000000
VERSION     DASZOB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151001"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VG709_07045"
     CDS             <1..292
                     /locus_tag="VG709_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWC32868.1"
                     /translation="AVLGDMAERGPGAPAYHREVGAAAARAGVEALVAVGPLARGYLE
                     GAGDIPEKRWAPTDRALPAVRAVLRPGDCVLVKASRSMGLEAVADALAVVPA"
     gene            314..1324
                     /gene="mraY"
                     /locus_tag="VG709_07050"
     CDS             314..1324
                     /gene="mraY"
                     /locus_tag="VG709_07050"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWC32869.1"
                     /translation="MEKVLLSGIIGMVIAIAAGPKFIEFLRRNELGQHIREEGPERHV
                     TKQGTPTMGGLLIMMAMAVPFLLFTERTLPALTAFFGTLGCAAIGFADDWTKLTHRRS
                     LGLAGRWKLLLLALITGVVALVVHDAGFKTSLYIPLVDLDVPLSWAWYGFLFVIIAGA
                     ATGVNLTDGLDGLAAGTATIALLTYTAMGVVAYLVSVRARSPDPEDLDLAIFGASLIG
                     ASIGFLWYNAFPAQVFMGDTGSMALGGALAAFAIMTKTELLLVLIGGIFVIEALSVIV
                     QVFTFKWLGRRVLLMAPLHHHFEMKAWSETRIMVRFWIAAAILCACAFVLYYRYFSEF
                     AA"
     gene            complement(1300..>2031)
                     /locus_tag="VG709_07055"
     CDS             complement(1300..>2031)
                     /locus_tag="VG709_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWC32870.1"
                     /translation="VLAEVAAARVDLVVCGGDVVLGPMPKESLQAVRSLGERVAFVRG
                     NADREVADAAPREAGGDVWARRAHWCAERLEREEIAFLAGLPPTVTVTVEPLGDVVFC
                     HGTPRSDEEIVTVVTPEARLCSLLADVRADVVVAGHTHTQLDRAAGAVRFVNAGSVGM
                     PYEREPGAYWALLGPPVDLRRTPYDTEAAAERIGATGYPEADELARENVLSVPSPDEA
                     APFFERMADERAGAAESGQAANSEK"
BASE COUNT          247 a          749 c          702 g          333 t
ORIGIN      
        1 ggcggtgctc ggcgacatgg ccgagcgcgg ccccggcgcg cctgcttacc accgggaggt
       61 gggtgcggcc gccgcgcgcg ccggagtcga ggcgctcgtc gcggtcggcc cgctcgcgcg
      121 cgggtacctc gagggcgccg gcgacatccc cgagaagcgg tgggcgccga ccgaccgggc
      181 gctgccggcc gtccgcgccg ttctgcggcc gggcgactgc gtcctcgtga aggcgtcccg
      241 gtcgatgggc ctcgaagcgg tcgccgacgc gctcgccgtc gtaccggcct agccctctgc
      301 ttgagtaacc gccgtggaga aggtcctcct ctccgggatc atcggcatgg tcatcgcgat
      361 cgcggcgggc ccgaagttca tcgagttcct gcgccggaac gagctcgggc agcacatccg
      421 cgaggagggg cccgagcggc acgtgaccaa gcagggcacg ccgacgatgg gcggtctcct
      481 gatcatgatg gccatggccg tcccctttct cctcttcacg gagcgcacgc ttccggcgct
      541 cacggctttc ttcggcaccc tcgggtgcgc agcgatcggt tttgccgacg actggacgaa
      601 gctgactcac cgccgctcgc tcggcctcgc cggccgctgg aagctcctct tgctcgcgct
      661 catcacgggc gtcgtcgccc tcgtcgtcca cgacgcgggc ttcaagacga gcctctacat
      721 cccgctcgtc gacctcgacg tgccgctctc gtgggcctgg tacggcttcc tcttcgtcat
      781 catcgcgggg gcggcgaccg gcgtgaacct gacggacggg ctcgacggcc ttgcagcggg
      841 cacggccacg atcgcgctcc tcacctacac ggcgatgggc gtcgtcgcct acctcgtcag
      901 cgtccgcgcg cgctcccccg acccggagga tctcgacctc gccatcttcg gcgcctcgct
      961 catcggggcc tcgatcgggt tcctctggta caacgcgttt cccgcgcagg tcttcatggg
     1021 cgacacgggc tccatggccc tcggcggggc gctcgccgcc ttcgcgatca tgacgaagac
     1081 ggaattgctg ctcgtcctca tcggcggaat cttcgtcatc gaggccctct cggtcatcgt
     1141 ccaggtgttc acgttcaagt ggctcgggcg gcgtgtcctg ctcatggcgc cgctccatca
     1201 ccatttcgag atgaaggcgt ggtcggagac gcggatcatg gtccgcttct ggatcgcggc
     1261 cgcgatcctg tgcgcgtgcg cgttcgtcct ctactaccgc tacttctcgg agttcgcggc
     1321 ctgaccgctc tcggcggccc cggcccgctc gtcggccatc cgctcgaaga acggagcggc
     1381 ctcgtcaggg ctcgggacgc tgaggacgtt ctcgcgtgcg agctcgtccg cttccgggta
     1441 tccggtggcg ccgattcgct ccgcggccgc ctcggtgtcg tacggcgtcc ggcgcaggtc
     1501 gacgggcggt ccgaggagcg cccagtacgc gcccggctct cgctcgtacg gcatgccgac
     1561 gcttccggcg ttcacgaagc ggacggctcc ggcggcgcgg tcgagctgcg tgtgcgtgtg
     1621 gccggcgacg acgacgtccg cgcgaacgtc cgcgaggagg gagcacaggc gcgcctcggg
     1681 cgtcacgacg gtgacgatct cctcgtcgct cctcggggtg ccgtggcaga agacgacgtc
     1741 cccgaggggc tcgacggtca ccgtgacggt cggtggcagt ccggcgagga aggcaatctc
     1801 ctcgcgttcg agcctctcgg cgcaccagtg ggcgcgccgg gcccacacgt cgccgcccgc
     1861 ctcgcgcggc gcggcgtcgg cgacctcgcg atcggcgttg ccccgcacga acgcgacgcg
     1921 ctccccgagc gagcgaacgg cctgcagcga ctccttcggc atcggcccca gcacgacgtc
     1981 gccgccgcag acgacgaggt cgacgcgcgc cgccgcgacc tccgcgagga c
//
LOCUS       DASZOB010000451         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_151027, whole genome shotgun sequence.
ACCESSION   DASZOB010000451 DASZOB010000000
VERSION     DASZOB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151027"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..812)
                     /locus_tag="VG709_07060"
     CDS             complement(69..812)
                     /locus_tag="VG709_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32871.1"
                     /translation="MRQSRLRIMTAGATARGWSTAIVAVVVLASALTVLVGRAVREES
                     NRPSIAVPPRNARPIAGRAVRWAVAEREAAMELPRLPEMELADPCTGGLASISLRRVW
                     SSGAGRDWHIGVNYTHGLWMTIAAAHRFNHARERELRPLRAAFPPFDFPEGLRDGEVR
                     GHVAWIGDLNPSFRCSRSATNPGRDWFIFDPARTGSITWKERAAVLELSGPYRAETLA
                     SLAGEAHWSRPHQPARRHVGRADRSRARS"
     gene            complement(822..>1509)
                     /locus_tag="VG709_07065"
     CDS             complement(822..>1509)
                     /locus_tag="VG709_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWC32872.1"
                     /translation="RDNVREALERLRAADAGLRIIVVDDASPDGTADVVLEAMARDGG
                     VALVERPPKGGLASAYLDGFRRALDQAADVVVEMDADLSHRPEDLGGLLDGIRRHHVV
                     IGSRYIPGGDVRNWSAFRRALSRGGNWYARLLLGFPVRDATSGYRVFRAEALRELISD
                     PIRSDGYGFQIELAYRAWRRGFSVGEVPITFVERRAGQSKLSRRIVVEALWQVMVWGV
                     RDRVLRRPYR"
BASE COUNT          232 a          531 c          539 g          207 t
ORIGIN      
        1 ggacgccgcc cggccgagct actttacgta atgttcgtta tagggaatat cctcgacggt
       61 cggagtgcct acgagcgtgc gcgactccga tccgccctac ccacgtgtcg gcgcgccggc
      121 tggtgaggcc gtgaccagtg cgcctcgccg gcgagagacg cgagggtctc ggctcgatac
      181 gggccggaaa gctcgaggac ggctgccctt tccttccacg tgatcgaccc cgtgcgagcg
      241 gggtcgaaga tgaaccagtc tcggcccggg ttggtcgccg agcgcgagca ccggaagctt
      301 gggttcagat cgccgatcca cgcaacgtgg ccccggacct cgccgtcgcg cagcccttcc
      361 gggaagtcga agggcggaaa ggcggcgcgg agcggccgaa gctccctctc gcgcgcgtgg
      421 ttgaacctgt gcgcagcggc gatcgtcatc cacagtccgt gggtgtagtt gacgccgatg
      481 tgccaatccc gccccgcgcc agacgaccag acgcgccgga gggagatgct ggctaggccg
      541 ccggtgcagg gatcggccag ttccatctcc gggaggcgcg gaagctccat ggctgcttcg
      601 cgctccgcga ccgcccacct cacggctcgc ccggcgatcg ggcgtgcgtt ccgtggcggg
      661 accgcgatcg agggacggtt cgactcctca cggaccgctc tgcccaccaa taccgtcaga
      721 gcagaggcga ggacgaccac cgcgacgatg gcggtcgacc acccacgcgc cgtggcgcct
      781 gcggtcatga ttcggagacg cgattgtcgc atcggcggtc ctcaccggta aggccggcgc
      841 agcacgcggt cgcggacgcc ccaaaccatc acctgccaca gcgcctcgac cacgatgcgg
      901 cgtgagagct tcgactgacc ggcgcggcgc tcgacgaacg tgatcggcac ctcgccgacg
      961 gagaagccgc gccgccatgc gcggtacgcg agctctatct ggaagccgta gccgtcgctg
     1021 cggatcgggt cggagatgag ctcgcgaagc gcctccgccc ggaagacccg gtatccgctg
     1081 gtggcgtcgc ggaccgggaa gcccaggagc agccgggcgt accagttccc gccgcgcgag
     1141 agagcccgcc ggaaggcgct ccagttgcgg acgtcgccgc cggggatgta gcggctcccg
     1201 atgacgacgt ggtgccggcg gatgccgtcg agaagcccgc ccaggtcctc gggacggtga
     1261 gacaggtcgg cgtccatctc gaccacgacg tcggcggcct ggtcgagcgc gcggcggaag
     1321 ccgtcgaggt acgccgaggc cagcccgccc ttgggggggc gctcgacgag cgcgacgccc
     1381 ccgtcgcggg ccatggcctc gaggacgacg tccgccgtcc cgtctggaga cgcgtcgtcg
     1441 acgacgatga tccgcagccc cgcgtcggcg gcccgcagcc gctcgagcgc ctctcggacg
     1501 ttgtcgcgc
//
LOCUS       DASZOB010000452         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_80383, whole genome shotgun sequence.
ACCESSION   DASZOB010000452 DASZOB010000000
VERSION     DASZOB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80383"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VG709_07070"
     CDS             complement(<1..1207)
                     /locus_tag="VG709_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32873.1"
                     /translation="MRKLLIGTAVLATLAAGAFPASGQVSTPVAPSENVEYESGDNTS
                     TGGHSVVEGNRLYVGAYGLGFRIFDISTPEDPQLIGGYTPGARADAVPDAAMYGDRHF
                     AVLNGTRRAQTRTTPVGEVRGTDISEFLDVTNPAAPRLLHVFAGQEHGEAHNGDIVDA
                     RRLWLPSGGAGPDGVWGLRIYSIARMTKTPAEQCRPGVPTNPCAPVRLFNGNPVELWR
                     NSPYRRGRPIGRPFTHTHDIEAYLKYPVHQPDGSFAARDIILLAEGGNYADDSGDTGS
                     AFVIDITDPRNPVVLLRWLHERGPGHHPIRYHHEAQFIDGETRVMLVTDEDLHNGCGN
                     AGGAVAVQLSADLTRAKELSEWFIPFGTPAPVCSVHVFSSNDSIVYFGSYNAGLQIVD
                     FSNPRAPRRV"
     gene            complement(1352..>3102)
                     /locus_tag="VG709_07075"
     CDS             complement(1352..>3102)
                     /locus_tag="VG709_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC32874.1"
                     /translation="LLLVLDNFERVMDAAPDVDRLLRAGPELRVLVTSRSPLQLYGEH
                     EFPVPPLSLPDLSHPTDAAQLSQFEAVALFIDRATAVKPGFAVTNDNAPAVAAICAGL
                     DGLPLAIELAASRVKVLPPSAILSRLQDRLSLLRSESRSLPERQRTLRGAIDWSYELL
                     DEAERRLLARLAVFAGGGTFDAIDVVANPDREIVPDTLDALGSLVDKSLLRQAEDPDG
                     EARFSMLETIREYAAERLRTSGEEREIRRRHAEHVASFAERAEPHLEGTEQRTWLDRC
                     EREHDNVRAALAWSLAAGEVEVGLRIAAALWRFWQQRGHLREGRRLLEDLLEQASGDR
                     TEIRAIAEIAAGSVVYWQGDYTAADRHYTNALEISHERGDRRGTMEALFNRAFIPLVT
                     GRFDDARPIFDEALAIARELGDRRVIALASGSIGYLLLRSEPDAAIPMLEEQVTAARE
                     LGNQFWEADGIVSIGQSHRFLGDPDSAERRYVEALHLYLEHGNLPMIGATLMFIAFVE
                     SMRGRHDRAMRLFAAGDRIMSDAGSTAPPEGMFFVDPEPPARDALGDDAVERALDEGR
                     AMDLDKAVAYALEDGG"
BASE COUNT          455 a         1091 c         1079 g          477 t
ORIGIN      
        1 agacgcgcct cggcgcgcgc gggttcgaga agtcgacgat ctgcagtccc gcgttgtacg
       61 agccgaagta cacgatgctg tcgttcgacg aaaacacgtg cacggagcac accggagccg
      121 gagttccgaa cgggatgaac cactccgaga gctccttcgc cctcgtgagg tccgcggaga
      181 gctgcacggc gacagcgccg cccgcgttcc cgcagccgtt gtgcaggtcc tcgtcggtga
      241 cgagcatcac gcgagtctcg ccgtcgatga actgcgcttc gtggtggtac cggatcgggt
      301 ggtgccccgg gcctcgttcg tggagccacc ggagcagcac gacggggtta cgggggtcgg
      361 tgatgtcgat gacgaacgcg ctccccgtgt cgcccgagtc gtcggcgtag ttcccgccct
      421 cggccagcag gatgatgtcg cgcgccgcga acgacccgtc gggctggtgg accgggtact
      481 tgaggtaggc ctcgatgtcg tgcgtgtggg tgaagggcct tccgatcgga cggcccctgc
      541 ggtacggtga gttcctccac agctcgaccg ggttgccgtt gaacaggcgg accggcgcgc
      601 acgggttcgt cggcacgccg ggccggcact gctccgccgg cgtcttcgtc atccgggcga
      661 tcgaatagat ccgcaggccc cagaccccgt ccgggccggc accgccggag ggaagccaca
      721 gtcgccgagc gtcgacgatg tccccgttgt gcgcctcgcc gtgctcctgg ccggcgaaca
      781 cgtgaaggag ccgcggcgcc gccgggttcg tcacgtcgag gaactcggag atgtcggtgc
      841 ccctcacctc gccgaccggc gtcgtgcgcg tctgcgctcg ccgggtgccg ttcagcaccg
      901 cgaagtgccg gtcgccgtac atggcggcgt cgggaacggc gtcggcccga gcgcccggtg
      961 tgtacccgcc gatgagctgc gggtcctcgg gcgtcgagat gtcgaagatc cggaacccga
     1021 ggccgtacgc gcccacgtac agccggttcc cctcgaccac cgaatgaccg ccggtcgacg
     1081 tgttgtcgcc gctctcgtac tcgacgttct cgctcggcgc cacaggcgtc gagacctgcc
     1141 ccgaggccgg gaacgcaccc gcggccaggg tggcgaggac ggcggtaccg atcagaagct
     1201 tccgcatcgc atctcccttc gcgaccgtac gcggaaccac tccatacgcg tattcggcgc
     1261 gaaccgcgcg cgttccttcc ccgcagaacg cccggccgcc cgtgtccccg gacgtcggag
     1321 cgcaacgcga cggtccatcg agagccccta ctcagccacc atcctcgagg gcgtacgcga
     1381 cggccttgtc gaggtccatc gcgcgcccct cgtcgagggc ccgctccacc gcgtcgtcgc
     1441 cgagcgcgtc ccgcgccggc ggctccggat ccacgaagaa catcccttcc gggggagccg
     1501 tgctccccgc gtcgctcatg atgcggtcgc cggcggcgaa gagccgcatc gcgcggtcgt
     1561 gccggccgcg catcgactcg acgaacgcga tgaacatgag cgtcgctccg atcatcggaa
     1621 ggttcccgtg ctccaggtac aggtggagcg cttcgacgta ccgccgctcc gcggaatccg
     1681 gatcaccgag gaaccggtgt gactgtccga tggagacgat gccgtccgct tcccagaact
     1741 ggtttcctag ctcgcgcgcc gccgtgacct gctcctcgag catcgggatc gccgcgtcgg
     1801 gctcgctccg gagcagtaag tacccgatcg aacccgacgc cagcgcgatg acgcgccggt
     1861 caccgagctc gcgtgcgatc gcgagcgcct cgtcgaagat cggtcgagcg tcgtcgaagc
     1921 gtccggtcac gagcgggatg aaggcgcggt tgaacagcgc ctccatcgtg ccgcgtcgat
     1981 ctcctcgctc gtgggagatc tcgagcgcgt tcgtgtagtg acggtcggcg gcggtgtagt
     2041 cgccctgcca gtacacgacg ctcccggccg cgatctcggc gatcgctcgg atctcggtcc
     2101 gatcgccgga tgcctgctcg agcagatcct cgaggagccg gcgaccctcg cggaggtgcc
     2161 ctcgttgctg ccagaaccgc cacagcgccg ctgcgatccg gagcccaacc tcgacctcgc
     2221 cggccgccag gctccatgcc agggcggcgc ggacgttgtc gtgctcgcgc tcgcaacgat
     2281 ccagccacgt ccgctgctcg gtcccctcga ggtgcggctc cgcgcgctcg gcgaacgagg
     2341 cgacgtgttc cgcgtgccgc cttcggatct ccctctcctc gcccgaggtc cggagacgct
     2401 cggccgcgta ttcgcggatc gtctccagca tcgagaaccg cgcctcgccg tcgggatcct
     2461 ccgcctgacg gagaaggctc ttgtcgacga gcgagccgag cgcgtcgagg gtgtcgggca
     2521 cgatctcccg atccgggttc gcgacgacgt cgatggcgtc gaacgtcccc cctcccgcga
     2581 agacggcgag acgggcgagg aggcgtcgct ccgcctcgtc gagcagctcg tagctccagt
     2641 cgatggcacc tcggagcgtt cgctggcgtt ccgggaggct tcgcgactcg ctcctcagga
     2701 gcgacaggcg gtcctgaagc cgggagagga tcgcagaggg cggcaagacc ttcactcggc
     2761 tcgctgcgag ctcgatcgcg agcgggagcc cgtcgagacc ggcgcagatc gcggcgaccg
     2821 ccggcgcgtt gtcgttcgtg accgcgaagc cgggcttcac cgcggtcgcg cggtcgatga
     2881 agagcgcgac ggcctcgaac tgcgagagct gcgccgcgtc ggtggggtgg ctgagatccg
     2941 gaagggaaag cggcggcacg gggaactcgt gctcgccgta gagctggagc ggcgatcggc
     3001 tcgtgacgag cacgcgcagc tccggtcccg cgcggagcag ccgatcgacg tccggggcgg
     3061 cgtccatgac gcgctcgaag ttgtcgagga ccagcagcag cg
//
LOCUS       DASZOB010000453         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_90574, whole genome shotgun sequence.
ACCESSION   DASZOB010000453 DASZOB010000000
VERSION     DASZOB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90574"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VG709_07080"
     CDS             <1..235
                     /locus_tag="VG709_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWC32875.1"
                     /translation="IPVEPQLARLDTVPARFADRGVWIAAVDDVRGSIEPLLDLADRD
                     EAGGLGDAPWPPHFPKGQREPTRVAPSRARKSR"
     gene            complement(420..1460)
                     /locus_tag="VG709_07085"
     CDS             complement(420..1460)
                     /locus_tag="VG709_07085"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWC32876.1"
                     /translation="MSVLPVKPPLEPMLAKLQPSLPRGDGWRYEPKWDGFRGIVFRDG
                     DDVHIGSRGRRPLHRYFPELLDPLRDALPDRCVVDGEIVVARPDGVLDFEWLQMRLHP
                     AESRVNMLAKKTPAAFVAFDLVAEGSSDLRKRPLSERWDRLSAVLKPMPTSRAMKAIL
                     SGKPRVVLTPLTEDTDEAERWFDELGEFGLDGIIAKHDDLRYEPRKRVMVKVKHERTI
                     DCVVGGYRVHKSGEGVGSLLLGLYDANGVLHHVGAAGAFPAKQRLAMLERLKPLEGGQ
                     SFAPETVLGGPSRWRAEEKEWIPVEPKLVCEVAYDHFQGKRFRHLAQFRRWRDDRDPR
                     TCTFDQQRPDHG"
     gene            complement(1457..>1751)
                     /locus_tag="VG709_07090"
     CDS             complement(1457..>1751)
                     /locus_tag="VG709_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32877.1"
                     /translation="AHRGQAAANADDARLVVGVVRELGRSYRFLRFGFAIATGAAFVA
                     TVWWATVGTTRWLLVSVGVTAVGAAGLLLDIRRRKRMTLAERLNLELLDRGIR"
BASE COUNT          263 a          630 c          612 g          246 t
ORIGIN      
        1 catcccagtc gagccgcagc tcgcgcggct cgacacggtg ccggcgcggt tcgccgatcg
       61 cggcgtgtgg atcgccgcgg tcgacgacgt ccgcggctcg atcgagccgt tactcgacct
      121 tgccgatcgc gacgaggccg gcggcctcgg cgacgcgccg tggccaccgc atttcccgaa
      181 ggggcaacgc gagccgacgc gcgtcgcgcc gtcgcgcgcc cgcaagagtc gctaggactc
      241 gtcgctcgag cggagatcga ggccttcgag gcccacgagc acgccgccga tcgcgcttga
      301 ggcgacaagg ggttcaggcg gctgcagcag ttgcacaggg aaccatgcga tgccgcccac
      361 gattccgacc cagatccctg cgggcgacca cgggagccgg cctgttcggg acgttcgcat
      421 cagccgtggt cggggcgctg ctggtcgaac gtgcaggtcc gcgggtcgcg gtcgtcgcgc
      481 cagcggcgga actgcgcgag gtgccggaag cgcttcccct ggaagtggtc gtacgcgacc
      541 tcgcagacga gcttcggctc gacagggatc cactccttct cctccgcgcg ccaccggctc
      601 gggccgccga gcaccgtctc gggcgcgaag ctctgaccgc cctcgagcgg cttcaggcgc
      661 tcgagcatcg cgaggcgctg cttcgcgggg aacgcgccgg ccgcgccgac gtggtgaagc
      721 acgccgttcg cgtcgtacag cccgaggagg agcgagccga cgccctctcc ggacttgtgg
      781 acgcggtaac ctccgacgac gcagtcgatc gtccgctcgt gcttcacctt caccatcacg
      841 cgcttccgcg gttcgtaccg gaggtcgtcg tgcttcgcga tgatcccgtc gagaccgaac
      901 tccccgagct cgtcgaacca gcgctcggcc tcgtcggtgt cctccgtgag cggcgtcagc
      961 acgacgcgcg gcttccccga gaggatcgcc ttcatcgcgc gcgaggtcgg catcggcttc
     1021 agcacggccg acagccggtc ccatcgctcc gacaggggac gcttgcggag atcgctcgat
     1081 ccctctgcga cgagatcgaa ggcgacgaag gccgccggcg tcttcttcgc gagcatgttc
     1141 acgcgcgact ccgcgggatg caggcgcatc tggagccact cgaagtcgag gacgccgtcg
     1201 ggccgagcca cgacgatctc cccgtcgacg acgcaccggt ccgggagggc gtcgcggagc
     1261 ggatcgagca gctctgggaa gtagcggtgg agagggcggc gaccgcggct gccgatgtgg
     1321 acgtcgtcgc cgtcgcggaa cacgatgccg cggaagccgt cccacttcgg ctcgtaccgc
     1381 cagccgtcgc cgcgcggcag cgacggctgc agcttcgcga gcatcggctc gagcggtggc
     1441 ttgacgggaa gcacgctcac cggatccctc ggtcgaggag ctccaggttg agtcgttccg
     1501 ccagcgtcat cctcttccga cggcggatgt cgagcagcag gcccgccgca cccaccgccg
     1561 tcacgccgac cgatacgaga agccagcggg tcgtccccac cgtcgcccac cagaccgtcg
     1621 ccacgaacgc ggcaccggta gcgatcgcga aaccgaagcg gaggaaccgg taggagcgac
     1681 cgagctcgcg gacgacgccg acgacgaggc gcgcgtcgtc ggcgttcgcc gccgcttgtc
     1741 cgcggtgcgc g
//
LOCUS       DASZOB010000454         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_80421, whole genome shotgun sequence.
ACCESSION   DASZOB010000454 DASZOB010000000
VERSION     DASZOB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80421"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VG709_07095"
     CDS             <1..1184
                     /locus_tag="VG709_07095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWC32878.1"
                     /translation="ARGETRPDGTPERILGTVQDVTEARRAEREHRIAATLQRSLLPE
                     RLPEIPGVELSARYVPATEESDVGGDWYDVVELPSGRIALAVGDVAGHGLRAASVMAQ
                     LRMALRAYAVEESGPADVIERVHRLFQRVSLSDMATVVYLVFDVESGEVRFANAGHPP
                     PLVIRRDGSTEYVGGALAPPLGAMPHAGRFFEGRASLSDGDTLLLFTDGLVERRGTAL
                     DEGLDRLAREAASARGGLETLCETLLTTLVGEGVADDVAMLAVRPVTLAERPFEIRIA
                     AEPHVLATIRRAVRRWLRAVDASEEDAYEVLAALGEACTNVIQHAYGAARGRLELRFD
                     RADGDIEVMVRDFGTWRGESRGDGGRGLVLMRGLMDAVSIERDAAGTTVTMRRRLRRR
                     S"
     gene            1255..1530
                     /locus_tag="VG709_07100"
                     /pseudo
     CDS             1255..1530
                     /locus_tag="VG709_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            complement(1516..>2664)
                     /locus_tag="VG709_07105"
     CDS             complement(1516..>2664)
                     /locus_tag="VG709_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32879.1"
                     /translation="VPASSLTGVVLFASATGVTAMDAATGSTLFARAGVPALGDWSTL
                     FATSAEGPRTSRETYDSGSGERLSTGRVPDGFGVRIAANDGSQVALVRGAPAPDDWTP
                     YPRATTDILVTDPSGVSPPERYLLEGNFDPEAFSSDGRRLFLLEYVPATAPTSYRVTT
                     LDLERGQVSPVFLSHEKRIAETMSGTRLEQLAPPDGRYLFTLYTSEPSAYLGDGSTVR
                     EPVAFVHVLDLGNGTAHCIALPEELWGGDPGVQAMAVSPSGDLLYVADTERGIVAEIA
                     TQRGKVRRVASVDFPRSDETHAVVTDRSRLVVASGPELVTVDAGALRPSARHDVGADV
                     RGLGTGDRGLLVSTKEGVEVLDPTTGETVDRLPARASSETAYVGSVDL"
BASE COUNT          380 a          911 c          990 g          383 t
ORIGIN      
        1 gggcgcgcgg cgagacgcgg cccgacggga cgcccgaacg catccttggg acggtgcagg
       61 acgtgaccga ggcgcgccgg gcggaacgtg agcaccggat cgccgcgacg ctgcagcgca
      121 gcctgctgcc cgagaggctc ccggagatcc ccggggtcga gctctcggcg cggtacgtgc
      181 cagccacgga ggagagcgac gtcggcggcg actggtacga cgtcgtcgag cttccgagcg
      241 gacggatcgc gcttgcggtc ggcgacgtcg ccggacacgg cctccgcgcc gcctccgtca
      301 tggcgcagct caggatggcg ctccgcgcgt acgccgtcga ggagtcggga ccggccgacg
      361 tcatagaacg cgtgcaccga ttgttccagc gggtgtccct gtcggacatg gccacggtcg
      421 tctatctcgt gttcgacgtg gagtccggcg aggtccggtt cgcgaatgcg ggacaccctc
      481 cgcctctcgt catccgtcgc gacggcagca ccgagtacgt cggcggggcg ctcgctccgc
      541 cgctcggcgc gatgccgcat gccggtcgct tcttcgaggg gcgagcgtcg ctctccgacg
      601 gagacacgct gctcctcttc accgatgggc tcgtcgagcg ccgagggacc gcactcgacg
      661 aggggctcga ccggctcgcc cgtgaagcgg catccgctcg cggcggtctc gagacgctct
      721 gcgagacgct gctcacgacg ctggtgggcg agggggtagc cgatgacgtc gcgatgctcg
      781 cggtgaggcc cgtcaccctc gccgagcgcc cattcgaaat acggatcgct gcggagccgc
      841 acgtgctcgc gacgatccgg cgtgccgtcc ggaggtggct ccgtgccgtc gacgcgtcgg
      901 aggaggacgc gtacgaggtc cttgcggcgc tcggcgaggc gtgcacgaac gtcatccagc
      961 acgcgtacgg cgcggcgcga ggacggctcg agctgcggtt cgatcgcgcc gacggcgaca
     1021 tcgaggtgat ggtgcgtgac ttcgggacgt ggcgtgggga gtcccgcggc gacggcggca
     1081 gagggctcgt cctcatgcgg gggctcatgg acgccgtctc gatcgaacgc gacgccgcgg
     1141 gaaccacggt cacgatgcgc cgccgcctgc gacggcgttc atgactgagc cgacgcgggt
     1201 cgaagcggaa cgcgtcgggc gcgtgtcggt ggtgcgggtc cggggagagg tagacaggcg
     1261 aatgcggcag atctcctgtc ggcgctcgag gcgagcgccc acgatgacgt ctccgccctc
     1321 gtcgtcgaca tctccgacgt cacctacgtc gacggtgctg gcgtgagcgt gctgttctcg
     1381 cttgcgcagc ggctgaggtc cacgcgccgc gcgctgcggg cgctgctgcc ggacggggct
     1441 ccgatccgcg cggcgctcga cgtcatggat ctcgcgaggg ccgtgcctat cctgccggat
     1501 ctcgagtccg cgctctcaga ggtcgactga cccgacatac gcggtctcgg agctcgcgcg
     1561 agcagggagc cgatcgacgg tctcgccggt ggtcgggtcg aggacctcga ccccctcttt
     1621 cgtcgagacg aggaggccgc ggtcaccggt tccgaggccg cgcacgtcgg cgccgacgtc
     1681 gtggcgcgcg gacggtcgaa gcgccccggc gtcgaccgtg acgagttcgg gccctgaggc
     1741 gaccacgagc cgcgagcgat ccgtcacgac ggcgtgcgtt tcatcggacc gggggaagtc
     1801 cacggacgcg acccgcctca ccttcccgcg ctgcgtcgcg atctccgcca cgatccctcg
     1861 ctccgtgtcc gcgacgtaga ggagatcgcc gctcggggag accgccatgg cctggacgcc
     1921 cggatcaccg ccccacagct cctcgggcag cgcgatgcag tgtgccgtcc cgttgcctag
     1981 atcgaggacg tggacgaacg cgacgggttc acggacggtc gacccgtcgc ccaagtacgc
     2041 ggaaggctcg ctcgtgtaca gcgtgaacag gtagcggcca tctggcggag cgagctgctc
     2101 gaggcgcgtc ccgctcatgg tctcggcgat gcgcttctcg tggctcagaa acaccggcga
     2161 gacttgccct cgctcgaggt ccagcgtcgt cacgcggtag gacgtcggtg ccgtcgcggg
     2221 gacgtactcg aggagaaaca gacgcctgcc gtccgaggag aacgcctccg ggtcgaagtt
     2281 cccctcgagg agatatcgct ccggcggcga cacgccggac gggtccgtga cgaggatgtc
     2341 ggtcgtcgcg cgcgggtacg gggtccagtc gtcgggcgcg ggggcgcctc gcacgagcgc
     2401 gacctgcgac ccgtcgttcg ccgcgatgcg aacgccgaag ccgtccggaa cgcggccggt
     2461 cgaaaggcgc tcgccgctgc ccgagtcgta cgtctcgcgg gaggtgcgcg gaccttcggc
     2521 cgacgtcgcg aacagcgtcg accagtcgcc gagcgccggc acccccgccc tcgcgaagag
     2581 cgtcgagccg gtcgccgcgt ccatcgccgt gacgccggtg gcagaggcga aaaggactac
     2641 gcccgtgagc gatgaggcgg gaac
//
LOCUS       DASZOB010000455         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_151154, whole genome shotgun sequence.
ACCESSION   DASZOB010000455 DASZOB010000000
VERSION     DASZOB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151154"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VG709_07110"
     CDS             complement(<1..581)
                     /locus_tag="VG709_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWC32880.1"
                     /translation="MARTSAASRAAALVPDGFRRAWRHRKEDVLLYAAGLGFYALISI
                     VPLAIVSMWIVSVFAGDSRVEQLAGGLSQVLPQGLGVDRAVRRVAQIGTSLGVTAVIA
                     ALWPATAYGAGLRRAFVRLRGANEKAEGFRGRGLVLLVLLPLLVLGTLLGAFAMTGLF
                     GEGLVRVLGWVLALALAFVGAGAALVVIYRVFPV"
     gene            complement(613..2217)
                     /locus_tag="VG709_07115"
     CDS             complement(613..2217)
                     /locus_tag="VG709_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC32881.1"
                     /translation="MARAVTDRPQAASRPDVLESFDPATGEVVGSVPITSPEAVEGIA
                     REVARIQKGWALVPLPERLRTMRNAVRVLVRRRPEIAMVITRENGKTISESAAAEVTD
                     AISNTDWVARYGAKYLAPERIPDPMPLLLPTKRHWVKFEPLGVVGIISPWNWPFMIPL
                     AEVAHAIAAGNGVILKPSEFTPLVGEEIAKVFAEAGVPDGLLRVIHGYGDTGAALCEA
                     GPVRKIFFTGSTTTGRKVMETAAKHMKPVVLELGGKDPAVVLADADIERAAEGILWNA
                     LGNCGQTCAGVERVYVDRRVYDRFVERLVAEARRVRPGDPKDPDTQIGPLNNDMQYRK
                     VLEQLEDAVEKGARVEVGGPEACAGLPDRYVAPTVLTGVDHSMRVMREETFGPLLPVM
                     AFDEEREAVELANDSDYGLGASVWTRDLRRGRMLAERIEAGMVWVNDHAYSHGFPQTP
                     WGGVKGSGSGGVTHSRFGLYEMVDKKLVADDRGLVPDPWWYPYSGLRSEGFIRIVDSL
                     YSEGRRVGTLWRNRRDLVSFVKDLRA"
     gene            complement(2240..>2443)
                     /locus_tag="VG709_07120"
     CDS             complement(2240..>2443)
                     /locus_tag="VG709_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWC32882.1"
                     /translation="AGLDGIEAAHSDHTPKMEARYREMARRLGLVATGSSDCHGTRYD
                     PVRLGSVTTDPEEFRRLEARRAA"
BASE COUNT          369 a          914 c          812 g          348 t
ORIGIN      
        1 accgggaaca cgcggtagat cacgacgagc gccgccccgg caccgacgaa cgccagagcg
       61 agcgccagca cccatccgag cacccgcact aggccttcgc cgaagagccc ggtcatcgcg
      121 aatgcgccga gcagcgtccc gaggactaag agcggaagga gcaccaggag gacgaggccg
      181 cgcccccgga agccctcagc cttctcgttc gcgccacgga gacggacgaa cgcgcgacgc
      241 agcccggccc cgtacgccgt cgccggccac agcgcggcga tgaccgcggt cacgccgagg
      301 ctcgtcccga tctgtgcgac gcgcctcacc gctctgtcga cgccgagacc ctgcggcaac
      361 acctgtgaga gccccccagc cagctgctcc accctggaat cgccggcgaa tacgctcacg
      421 atccacatcg acacgatcgc cagcgggacg atcgagatga gagcgtagaa accgagcccc
      481 gcggcgtaga gcaggacgtc ttccttgcgg tggcgccacg cgcgccgaaa cccgtcgggc
      541 acgagggcgg ccgctcgcga cgcggcactc gtccgcgcca tcagcgagca cttgtaccca
      601 gcgaagctgg cgctacgccc ggagatcttt cacgaacgag acgaggtcgc ggcggttgcg
      661 ccagagggtc ccgacgcggc ggccctcgga gtacagcgaa tctacgatcc tgatgaagcc
      721 ctcgctgcgg aggccgctgt acgggtacca ccatgggtcg gggacgagcc cgcggtcgtc
      781 ggcgaccagc ttcttgtcga ccatctcgta gagcccgaac ctcgagtggg tcacgccgcc
      841 ggagccggag cccttcacgc cgccccacgg cgtctgcggg aacccgtgcg agtacgcgtg
      901 atcgttgacc cacaccatcc cggcctcgat ccgctctgcg agcatccgcc cgcgtcgaag
      961 gtcgcgcgtc cagacgctcg cacccagccc gtagtcggag tcgttcgcga gctcgaccgc
     1021 ctctcgctcc tcgtcgaacg ccatcaccgg gagcagcggc ccgaacgtct cctcgcgcat
     1081 gactcgcatc gagtggtcga cgcccgtgag gaccgtcggc gcgacgtacc ggtccggcaa
     1141 gcccgcgcac gcctccgggc cgccgacctc gaccctcgcg cccttctcga cggcgtcctc
     1201 gagctgctcg agcaccttcc ggtactgcat gtcgttgttg agcggcccga tctgcgtgtc
     1261 ggggtccttg gggtcgcccg ggcgaacccg gcgcgcctcg gcgacgaggc gttccacgaa
     1321 ccggtcgtag acccgccggt cgacgtagac gcgctcgacg cccgcgcacg tctggccgca
     1381 gttgccgagc gcgttccaga ggatcccttc ggccgcgcgc tcgatgtcgg cgtcggcaag
     1441 cacgaccgcg gggtccttcc cgcccagctc gagcacgacc ggcttcatgt gcttggccgc
     1501 cgtctccatc accttgcggc ccgtcgtcgt gctccccgtg aagaagatct tgcgcacggg
     1561 ccccgcctcg cagagcgcgg cgcccgtgtc cccgtagccg tggatcaccc gcagcagccc
     1621 atctgggacg ccggcttccg cgaagacctt cgcgatctcc tcgccgacga gcggcgtgaa
     1681 ctccgacggc ttgaggatga cgccgttgcc ggccgcgatg gcgtgcgcga cctcggcgag
     1741 cgggatcatg aacggccagt tccacggcga gatgattccg acgacgccga gcggctcgaa
     1801 cttcacccag tgccgcttcg tcggaaggag gagcggcatg gggtcgggga tccgctcggg
     1861 cgccaggtac ttcgcgccgt agcgcgcgac ccagtcggtg ttcgagatcg cgtcggtcac
     1921 ctcggccgcg gccgactccg agatcgtctt gccgttctcc cgcgtgatca ccatcgcgat
     1981 ctcagggcgg cggcggacga ggacccggac ggcgttccgc atggtccgca gccgctcggg
     2041 aagcggtacc agcgcccatc ccttctggat gcgcgcgacc tctcgcgcga tgccctcgac
     2101 ggcctcgggg ctcgtgatcg ggaccgagcc gacgacctcg ccggtcgcgg ggtcgaagct
     2161 ctcgaggacg tcgggacggg acgctgcctg cgggcgatct gtcacggcac gggccatgtg
     2221 gggcgctcct tctctcgcgc tacgccgcgc gcctcgcctc gaggcgccgg aactcctccg
     2281 ggtccgtcgt caccgaacca agccgaacgg gatcgtagcg cgtcccgtgg cagtcggacg
     2341 agccggtcgc gacgaggccg agccgccgcg ccatctcgcg gtaccgtgcc tccatcttcg
     2401 gcgtgtggtc cgagtgtgcg gcctcgatgc cgtcgagccc ggc
//
LOCUS       DASZOB010000456         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_90733, whole genome shotgun sequence.
ACCESSION   DASZOB010000456 DASZOB010000000
VERSION     DASZOB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90733"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VG709_07125"
     CDS             complement(<1..330)
                     /locus_tag="VG709_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32883.1"
                     /translation="MTKLLVAGLAVLAAVAVADTIRRESAASTAPSSASAPPQRVVHP
                     VTSGFVAVGPGTRTRVLLNGREYVSARQVDAAFPAPLEGVPFDIAHVASASDGTVALA
                     VYKIPVTG"
     gene            complement(327..>1690)
                     /locus_tag="VG709_07130"
     CDS             complement(327..>1690)
                     /locus_tag="VG709_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWC32884.1"
                     /translation="SMSFMSVTLTGRSPYERVLTYEKLRDEHGREMHRSWGNAIDAAE
                     ALDNMGADVMRWMFCEQVPSQNLKFGYGPAADIRRRLLTLWNSAAFLDTYATIEGFRP
                     RYDDLVEGPLTGTLRPLDRWLLARTQQLVGDIAAAYEAYWTPAITRLFEEFTDDLSNW
                     YIRRSRRRFYAHDEAAFRTLWYALTQAVRVIAPVMPFLAEHLWQNLVAASDRGAPESV
                     FLAGWPEPLEALGDEALIEEIAAARRVAELARSARQQAGLKLRQPLRRLVVATPDAGR
                     RALVSRQVDDLAGELRVKEVAIAGDPQEVADRRALARLDVVGPRLGPNLAELRRLLAE
                     GKFEVVEGSLRADGFVLAPGEYSLDYTAKEGWAVEHDDGYVVAVDTRLDPELELEGRV
                     LDVIHAVQRARRDAGLEITDRIVLTIAERDREVLAHEEWIKGETLATRVELGGELAVR
                     KDR"
BASE COUNT          239 a          594 c          619 g          238 t
ORIGIN      
        1 gcccgtcacc gggatcttgt agacggcgag cgcgaccgtc ccgtcggagg cggacgcgac
       61 gtgggcgatg tcgaacggga cgccttcgag cggcgcgggg aaggcggcgt cgacctgcct
      121 cgcgctcacg tactcgcgcc cgttcagcag gacgcgcgtg cgcgttcccg gcccgaccgc
      181 gacgaacccc gacgtgacgg gatggacgac gcgctgcggc ggggcggacg ccgagcttgg
      241 cgcggtgctc gccgccgact cgcgccggat cgtgtccgcc acggcgactg ctgcgagaac
      301 ggcgaggccg gcgacgagga gcttcgtcac cggtccttgc ggacggcgag ctcgcccccg
      361 agctcgacgc gcgtcgcgag cgtctcgccc ttgatccact cttcgtgcgc gaggacttcg
      421 cggtcgcgct cggcgatcgt caggacgatg cggtcggtga tctcgagccc ggcgtcccta
      481 cgcgcccgct ggacggcgtg gatcacgtcg aggacgcgcc cctcgagctc aagctcggga
      541 tcgaggcgcg tgtcgacggc gacgacgtac ccgtcgtcgt gctcgaccgc ccacccctcc
      601 ttcgccgtgt agtcgagcga gtactcgccg ggcgcgagga cgaacccgtc cgcgcggagc
      661 gagccctcga ccacctcgaa cttgccctcg gcgagcagcc gacgcagctc ggcgagattg
      721 ggcccgagcc gtgggccgac gacgtcgagg cgggcaagcg cgcgccggtc ggcgacctcc
      781 tgcgggtcgc cggcgatcgc cacttccttg acgcggagct ccccggccag gtcgtccact
      841 tggcgcgaga cgagcgcgcg tctccccgcg tccggcgtcg ccacgacgag gcgccggagc
      901 ggctggcgca gtttcaggcc cgcctgctga cgcgcggagc gggcgagctc ggcgacccga
      961 cgcgcggcgg cgatctcctc gatcagcgcc tcgtcgccga gcgcctcgag gggctcgggc
     1021 cagccggcga ggaagacgct ctccggcgca ccgcgatcgc ttgcggcgac gaggttctgc
     1081 cacaggtgct ccgcgaggaa cggcatgacg ggcgctatta cccgaacggc ctgcgtgagt
     1141 gcgtaccaga gcgtccggaa cgccgcctcg tcgtgggcgt agaaccgcct gcgcgagcgg
     1201 cggatgtacc agttcgagag gtcgtcggtg aactcctcga agaggcgggt gatcgccggc
     1261 gtccagtacg cctcgtacgc cgccgcgatg tcgccgacga gttgctgcgt ccgcgcgagc
     1321 agccagcggt cgagcggacg gagagtaccg gtaagcgggc cctcgaccag gtcgtcgtac
     1381 cgcggacgaa agccctcgat ggtcgcgtac gtgtcgagga aggcggcgga gttccagagc
     1441 gtcagcaggc gccgccggat atcggcggcg ggcccgtagc cgaacttgag gttctggctc
     1501 ggcacctgct cgcagaacat ccagcgcatg acgtctgcgc ccatgttgtc gagcgcctcc
     1561 gcggcgtcga tcgcgttccc ccacgatcgg tgcatctcgc gcccgtgctc gtcgcggagc
     1621 ttctcgtagg tgaggacgcg ctcatacggc gagcggccgg tgagcgtcac ggacatgaac
     1681 gacatcgagt
//
LOCUS       DASZOB010000457         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_45274, whole genome shotgun sequence.
ACCESSION   DASZOB010000457 DASZOB010000000
VERSION     DASZOB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45274"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VG709_07135"
     CDS             complement(<1..669)
                     /locus_tag="VG709_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HWC32885.1"
                     /translation="MDGRLMLKISGELGTKSARTRRRFLRILADNVRAALDRDGVGGV
                     VRPQWARLFVETGDAPGAREALARVFGLHSVSEVATLRFSTLDELVDEAASLYRDRVA
                     GRTFAVRVKRSGDHGFKSQEVARRLGSALLDSSAGVDLDVPEVEVPLEIVDGRAFAVL
                     RSEDGPGGLPLGASGRVVALFSGGFDSPVATWMAMRRGTEVDLVVCDLGGCGQVDVAL
                     HVARA"
     gene            complement(706..1173)
                     /locus_tag="VG709_07140"
     CDS             complement(706..1173)
                     /locus_tag="VG709_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWC32886.1"
                     /translation="MGEPQAPREKRPRPRDELGRPLPWDAENLLELEDFDALPLEENH
                     RLGIAHFDAGRFFQAHEAWETAWKQAKGEDDEEFFKGLSQLGAGYVHYRRGNAHGAHT
                     LIRRGLSRIRPYGPSHRGVAVEAVADAAEAHAERFEAAARSDGGLPEVTPPTI"
     gene            1215..>1701
                     /locus_tag="VG709_07145"
     CDS             1215..>1701
                     /locus_tag="VG709_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWC32887.1"
                     /translation="MGEALLTDLYEINMAASYLRRGMMEPATFSLFVRALPRGWGFFV
                     SCGLERCLDYLERLRFTDEDLEYLRREQGYPDDVLESLAGLRFTGDVWAIPEGRVFFA
                     EEPLLEVTAPLPEAQIVETFLLNQLTYQTAIASKAARCRIAAPEKSLVDFAFRRVHGT
                     EA"
BASE COUNT          237 a          635 c          599 g          230 t
ORIGIN      
        1 cgcgcgcgcg acgtgcagcg cgacgtcgac ctggccacag ccgccgagat cgcagacgac
       61 gaggtccacc tcggtcccgc ggcgcatcgc catccacgtc gcgaccggcg agtcgaaccc
      121 gcccgagaac agcgcgacga cgcggccgct cgcgccgagc ggcagcccgc ccggcccgtc
      181 ttcggagcgg agcaccgcga acgcgcgtcc gtcgacgatc tcgagcggca cctcgacctc
      241 cggcacgtcg aggtcgacgc cggcggagga gtcgagcagg gcggaaccga gccggcgcgc
      301 gacctcctgt gacttgaagc cgtggtcgcc cgagcgcttc acgcgcacgg cgaacgtccg
      361 gccggcgacg cggtcgcggt agagcgacgc ggcctcgtcg acgagctcgt cgagcgtcga
      421 gaaccgcagc gtcgcgacct cggagaccga atggaggccg aacacccgcg ccagcgcctc
      481 gcgcgccccc ggcgcgtctc cggtctcgac gaacagccgc gcccactgcg gacgcacgac
      541 gccgccgacg ccgtcacggt cgagcgcggc gcggacgttg tcggcgagga tccggaggaa
      601 gcgacgacgg gtgcgcgcgg acttcgtccc gagctcgccg gagatcttga gcatgaggcg
      661 gccgtccatc gcctcctcat ggtagccgag cgcgcggagc tggcctcaga tcgtcggcgg
      721 cgtgacttcg gggaggccgc cgtccgagcg cgccgcggcc tcgaaccgct ccgcgtgcgc
      781 ctcggcggcg tcggcgaccg cctcgacagc gacgccgcgg tgcgacggcc cgtacgggcg
      841 gatgcgcgac agcccgcgcc ggatcagcgt gtgcgcgccg tgcgcgttgc cccggcggta
      901 gtggacgtac cccgcgccga gctgcgacag gcccttgaag aactcctcgt cgtcctcgcc
      961 cttcgcctgc ttccacgcgg tctcccacgc ctcgtgcgcc tggaagaagc ggccggcgtc
     1021 gaaatgcgcg atgccgaggc ggtggttctc ctcgagcggc agcgcgtcga agtcctcgag
     1081 ctccaggagg ttctccgcat cccacgggag cggacgcccg agctcgtccc tcgggcgcgg
     1141 acgtttctcg cgtggcgcct ggggctcccc cacacgccca tgctagccgc ggcgccccga
     1201 ctatcctcgc cccgatgggc gaggcgctgc tcaccgacct ctacgagatc aacatggccg
     1261 cgagctacct gcgccgcggg atgatggagc cggcgacgtt cagcctgttc gtgcgcgcgc
     1321 tcccacgcgg ctggggcttc ttcgtgtcgt gcggcctcga gcgatgcctc gactacctcg
     1381 aacggcttcg gttcaccgac gaagacctcg agtacctgcg acgcgagcag ggctatcccg
     1441 acgacgtgct ggagtcgctc gccggcctcc ggttcaccgg cgacgtgtgg gcgatcccgg
     1501 aaggccgcgt tttcttcgcc gaggagccgc tccttgaggt gacggcgccg ctccccgagg
     1561 cgcagatcgt cgagacgttc ctcttgaatc agctcacgta ccagacggcg atcgcttcga
     1621 aggcggcccg gtgccggatc gcggcgccgg agaagtcgct cgtcgacttc gcgttccgcc
     1681 gcgtgcacgg gaccgaggcg g
//
LOCUS       DASZOB010000458         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_171437, whole genome shotgun sequence.
ACCESSION   DASZOB010000458 DASZOB010000000
VERSION     DASZOB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171437"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VG709_07150"
     CDS             <1..765
                     /locus_tag="VG709_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWC32888.1"
                     /translation="GDEWVIDGEKWFITGAHGAAFAIVVVLTDPDAENRYARHSLFLI
                     DAENPGWNVVREIPTMGSHLPGGDCEVRLEECRVPADAMLGARGAGLALAQARLGPAR
                     LAHAMRWVGVAQRALDLAAERALGREAFGKPLARHEAVQWMLADSAMQLYAARLMVLH
                     AAWKIEHGMDFRQEVAFVKVFVSEALGDIVDRALQIHGSLGYSGDLPLERFYRDARAA
                     RIYDGPSEVHRMVIARNLLKAAAADGTTRRATGGLA"
     gene            782..1102
                     /locus_tag="VG709_07155"
     CDS             782..1102
                     /locus_tag="VG709_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32889.1"
                     /translation="MGRTYEEERTFAHPKTTVFDAAQRAIPTIKRMRVKQADAASGRL
                     VVTKGMTLKSWGEDVSIVVEDDGPDGSRVRVASRVKAQLMDWGTNRQNVESVLDAIAL
                     QLGG"
     gene            1099..2163
                     /locus_tag="VG709_07160"
     CDS             1099..2163
                     /locus_tag="VG709_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWC32890.1"
                     /translation="MSEAVHGPPRDDAARPLLDPEPLTRYLDARLPGDGSFEVERHQA
                     GHSNETFFLRRSGMEWVLRRPPRGAFLPTAHDVLREHRVLSALAATPVRAPRPLVACD
                     DPSVIGAPFYVMEKVDGVVVRDVLPQVLADEAERRRVGEELIDRLAELHAVDWRDVDL
                     EGWGKPSGYLERQIKRWSGQLELATRFTRPLPDLERVRDWLGDHRPESPPPVIVHGDY
                     KLDNVVLSREVPVRVLAVLDWEMSTLGDPFADLGYLFSFWREPGDPPDAVLTEQVELT
                     RLPGFSSRAELVERYAERTGRDPGDTTWYVVLAIWKLAILLEGSYARHLAGVTDDPFF
                     AELEHGVPALARRAAEAADA"
     gene            2156..2827
                     /locus_tag="VG709_07165"
     CDS             2156..2827
                     /locus_tag="VG709_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWC32891.1"
                     /translation="MRERYAALVIDFGGVLTTSLRESFRKFCEHEGIEFERLRSLVRT
                     SYQVNDPEDLVAKIETGRISVEEFERQLAEIFSEGMREPIVAEGLLARMSENLELEPA
                     IIGVVRAAHDAGVKTALLSNSWGMDLYPNELLEELFDEIVISGQVGKRKPDAEVFHLA
                     ADGLEVEPTECVFVDDVRTNIEAAEAVGMRGVLHDDVDETVAQLEGLLGMRLREAGSD
                     AVGRL"
     gene            2831..>3053
                     /locus_tag="VG709_07170"
     CDS             2831..>3053
                     /locus_tag="VG709_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32892.1"
                     /translation="MDELTTPKGLAEHLDDVQVVDVREPLRQYATTGFVGSRPSTRPA
                     IRSCSGMWTSAQRILPASRSFGSRTSTTCT"
BASE COUNT          449 a         1019 c         1131 g          454 t
ORIGIN      
        1 ggggacgagt gggtgatcga cggcgagaag tggttcatca cgggcgcgca cggcgccgcc
       61 ttcgcgatcg tcgtcgtgct caccgatccg gacgccgaga accgctacgc gcgccactcg
      121 ctgttcctga tcgacgccga gaaccccggg tggaacgtcg tgcgcgagat cccgacgatg
      181 ggctcgcatc tcccgggcgg cgactgcgag gtccggctcg aagagtgccg cgtccccgcc
      241 gacgcgatgc tcggggcgag gggcgcgggt ctggcgctcg cgcaggcgcg gctcgggcca
      301 gcgcggctcg cacacgcgat gcggtgggtc ggggtcgcac agcgggcgct cgacctcgcc
      361 gccgagcgcg cgctcggtcg cgaggcgttc ggaaagccgc tcgcgcggca cgaggccgtg
      421 cagtggatgc tcgcagactc cgcgatgcag ctctacgccg cgcgactcat ggtgctgcac
      481 gccgcgtgga agatcgagca cggcatggac ttccgccagg aggtcgcctt cgtgaaggtg
      541 ttcgtctcgg aggcgctcgg cgacatcgtc gaccgcgcgc tgcagatcca cggctcgctc
      601 gggtactcgg gcgacctgcc gctcgagcgg ttctaccggg acgctcgggc ggcgcggatc
      661 tacgacggcc cgtcggaagt ccaccggatg gtgatcgcgc ggaacctgct caaggccgcg
      721 gcggccgacg ggacgacgcg tcgcgcgacc ggcggcctcg cgtgaacaag gaggacgtgt
      781 gatggggagg acgtacgagg aggagcgcac gttcgcgcac ccgaagacga cggtattcga
      841 cgcggctcag cgcgcgatcc cgacgatcaa gcggatgcgc gtgaagcagg ccgacgcagc
      901 gagcggacgc ctcgtcgtga cgaaggggat gacgctgaaa tcgtggggtg aggacgtgtc
      961 gatcgtggtg gaggacgacg gccccgacgg gtcgcgcgtg cgggtggcct cgcgcgtgaa
     1021 ggcgcagctc atggactggg gcacgaaccg gcagaacgtc gagtcggtgc tggacgcgat
     1081 cgcgctccag ctcggcggtt gagcgaggcc gtccacggcc cgccgcgcga cgacgccgcc
     1141 cggccgctcc tagatcccga gccgctcacg cgatacctcg acgcgcggct tcccggcgac
     1201 ggctcgttcg aggtcgagcg ccatcaggcc gggcactcga acgagacctt cttcctccgg
     1261 cgcagtggca tggagtgggt cctgcggcgc ccgccccgcg gcgcgttcct ccccaccgcg
     1321 cacgacgtgc tgcgagagca ccgcgtcctt tccgcgctcg ccgccacgcc ggtgcgcgcg
     1381 ccgcgtccgc tggtcgcgtg cgacgacccg tccgtgatcg gcgcgccgtt ctacgtgatg
     1441 gaaaaggtcg acggcgtcgt ggtccgcgac gtgcttcccc aggtgctcgc cgacgaggcg
     1501 gaacggcgac gcgtcggcga ggagctcatc gaccggctcg cggagctcca cgccgtcgac
     1561 tggcgcgacg tcgacctcga gggatgggga aagcccagcg gctacctcga gcgccagatc
     1621 aagcgctgga gcgggcagct ggagctcgcg acgcggttca cgcgcccgct tccggacctc
     1681 gagcgcgtcc gagactggct cggcgatcac cgccccgagt cgccgccacc cgtgatcgtc
     1741 cacggggact acaagctcga caacgtcgtg ctgtcgcgcg aggtgcccgt gcgggttctc
     1801 gccgtcctcg attgggagat gtcgacgctc ggcgacccgt tcgcggacct cgggtacctc
     1861 ttctcgtttt ggcgggagcc cggcgacccg cctgacgcgg tgctcaccga gcaggtcgag
     1921 ctcacccgtc tgccggggtt ctcgtcgcgg gcggagctcg tcgagcgtta cgcggagcgg
     1981 accggccggg atcccggcga cacgacctgg tacgtcgtgc tcgcgatctg gaagctggcg
     2041 atcctcctcg aggggtcgta cgcgcgtcac ctcgcgggag tgaccgacga cccgttcttc
     2101 gcggagctcg agcacggcgt gccggcgctc gcgcgtcgcg ccgcggaggc cgccgatgcg
     2161 tgagcgctac gccgcgctcg tgatcgattt cggcggcgtg ctcacgacgt cgctgcgcga
     2221 gtcgttccgg aagttctgcg agcacgaggg gatcgagttc gagcggctgc gctcgctcgt
     2281 acggacgtcg taccaggtca acgacccgga ggacctcgtg gcgaagattg agacgggccg
     2341 gatctccgtc gaggagttcg agcggcagct cgccgagatc ttctcggagg ggatgcgcga
     2401 gccgatcgtc gccgaagggc tcctcgcgag gatgagtgag aacctcgagc tcgagcccgc
     2461 gatcatcggc gtggtgcgcg cggcgcacga cgccggcgtg aagacggcgc tcctctcgaa
     2521 ctcctggggg atggatctct acccgaacga actgctcgaa gagctcttcg acgagatcgt
     2581 gatctccggg caggtcggca agcggaagcc cgatgccgag gtgttccacc tcgccgccga
     2641 cgggctcgag gtcgagccga ccgagtgcgt gttcgtcgac gacgtgcgga cgaacatcga
     2701 ggcggccgag gccgtcggga tgcgtggcgt cctgcacgac gacgtcgacg agaccgtggc
     2761 gcaactcgag ggtctgctcg gtatgaggct ccgcgaagcc ggatccgacg ccgttggtag
     2821 gctgtagcgg atggatgagc tgacgacgcc gaaaggtctc gcggaacacc tcgacgatgt
     2881 gcaggtcgtc gatgtgcgcg agccgctgcg gcagtacgcg acgacgggct tcgtcgggtc
     2941 gaggccttcg acgcgcccgg ccatcaggtc ttgcagcggg atgtggacgt cggcgcagcg
     3001 gatccttccg gcctcgcgct cgttcggctc gcgcacatcg acgacctgca cat
//
LOCUS       DASZOB010000459         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_171440, whole genome shotgun sequence.
ACCESSION   DASZOB010000459 DASZOB010000000
VERSION     DASZOB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171440"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VG709_07175"
     CDS             complement(<1..196)
                     /locus_tag="VG709_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32893.1"
                     /translation="MPPVSSGPAVRVDDASRIVERVGFFGEGADRLFGVLHAPSDGAR
                     AGVVVCPPVYAEFEKNYRSEV"
     gene            complement(208..1158)
                     /locus_tag="VG709_07180"
     CDS             complement(208..1158)
                     /locus_tag="VG709_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWC32894.1"
                     /translation="MTRAVVTGGAGFLGSHLCRRLLDEGWDVVCLDSLLTGRVDNVAE
                     LLGHPAFRFEQYDVTNYVFVEGDVDWVLHFASPASPLDYLEHPIHTLKVGALGTLRAL
                     GLAKDRGAGFLLASTSEVYGDPKVHPQPESYLGNVDPVSPRGVYDEAKRYGEALAAAY
                     RRVHSVPIRIARIFNTYGPRMRRRDGRAVPTFIRQALAGEPLTVHGDGSQTRSLCYVD
                     DLIEGFWRLLGSRIEDPVNLGTPEEVSVLALAETIRDLVGSSSDIGFTGRPVGDPEVR
                     CPDISLARERLGWEPGVPLREGLERTIAWARTAWAPEIPV"
     gene            complement(1155..2411)
                     /locus_tag="VG709_07185"
     CDS             complement(1155..2411)
                     /locus_tag="VG709_07185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWC32895.1"
                     /translation="MKVGIVGAGHVGLPTAGAFARVGHEVVVTDVDHEKIALLERGAM
                     PFFEPGLEELLREGILAKRISFTSDTGEAVAGAPVVFICVGTPPRRDGEANLAAVERS
                     AEDVARYASEPLVVVEKSTVPFGTGDRVRETMRRVRPDLEFQVVCNPEFLREGSAVED
                     SLRPGRILVGTSSAEARDAMRQLYEPFVEAGATWIETDIATAEIAKHASNAFLALKVS
                     YANALARICELAGADVLKVADIMGADPRIGRAHLTAGLGYGGSCFPKDLAAFDRLSRR
                     LGYEFPILAEVARINEEIIDETFRKIEAALWNLEEKRVALLGLAFKPGTDDVRFAPAL
                     ALAQRLLDAGVSVVGYDPQAAAGAKAEVPAMELAGDPYSALDGAHCAVLCTEWPELRA
                     LDPDTFVAAGFTYVPLGRPTLAPSRT"
     gene            complement(2408..>2687)
                     /locus_tag="VG709_07190"
     CDS             complement(2408..>2687)
                     /locus_tag="VG709_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS biosynthesis protein WbpP"
                     /protein_id="HWC32896.1"
                     /translation="DERVDGAVINIAGGDPKTVNEVLQAVSDVVGTWIEPERQPQRAG
                     DVRKTHADISRARELLGFEPQARWEESVRATVRWFTEGARPGAAETGR"
BASE COUNT          389 a          990 c          952 g          356 t
ORIGIN      
        1 gcacctcgct ccggtagttc ttctcgaact cggcgtagac cggcgggcag acgacgacgc
       61 cggcgcgggc gccgtccgac ggggcgtgga ggacgccgaa gagccggtcg gcaccttcgc
      121 cgaagaaccc gacgcgctcg acgatccgcg aggcgtcgtc gacgcgaacg gcgggacccg
      181 aggagacagg gggcacgacc ggcgattcta tacggggatc tccggtgccc aagccgtcct
      241 cgcccaggcg atcgtgcgct cgagcccctc gcgcagcggg acgcccggct cccacccgag
      301 gcgctcgcgc gccagcgaga tgtcggggca gcggacctcg ggatcgccga ccggcctgcc
      361 ggtgaacccg atgtcggaac tcgacccgac gaggtcccgg atcgtctcgg cgagcgcgag
      421 gacgctgacc tcctcgggag tgccgaggtt gacgggatcc tcgatccgcg agccgaggag
      481 cctccagaac ccctcgatga ggtcgtcgac gtagcagagc gagcgcgtct gcgacccgtc
      541 gccgtgcacg gtgagcggct cccccgccag cgcctgccgg atgaacgtag gaacggcacg
      601 gccgtcgcgg cggcgcatcc ggggcccgta ggtgttgaag atccgcgcga tccggatcgg
      661 gaccgagtgc acgcgccggt acgcggcagc gagcgcctcc ccgtaccgct tcgcctcgtc
      721 gtagacgcct cgcgggctga cggggtcgac gttgccgagg tacgactccg gctgcgggtg
      781 caccttcggg tcgccgtaga cctcggaggt cgaggcgagc aggaagccgg cgccgcggtc
      841 cttcgcgagc cccagggcac gcagcgtccc gagcgccccg accttcagcg tgtggatcgg
      901 gtgctcgagg tagtcgagcg gcgacgcggg cgaggcgaag tgcagcaccc agtcgacgtc
      961 gccctccacg aacacgtagt tcgtgacgtc gtactgctcg aagcggaacg ccgggtggcc
     1021 gagcagctcg gcgacgttgt ccacacgccc cgtgaggagc gagtcgaggc agacgacgtc
     1081 ccacccctcg tcgagcaagc ggcggcagag gtgggaaccg aggaacccgg cgccgcccgt
     1141 gacgacggcg cgggtcacgt ccgcgacggg gcgagggtcg ggcggccgag cgggacgtac
     1201 gtgaacccgg ccgcgacgaa cgtgtccggg tcgagcgcgc ggagctccgg ccactccgtg
     1261 cagagcaccg cgcagtgcgc tccgtcgagc gccgagtacg ggtccccggc gagctccatc
     1321 gccggcacct ccgccttcgc gccggcggcc gcctgcgggt cgtagccgac gaccgacacg
     1381 ccggcgtcga ggaggcgctg cgccagggcc agcgccggcg cgaaccgcac gtcgtcggtc
     1441 ccgggcttga acgcgagccc cagcagcgcc acgcgcttct cctcgaggtt ccacagcgcg
     1501 gcctcgatct tgcggaacgt ctcgtcgatg atctcttcgt tgatccgtgc gacctccgcg
     1561 aggatcggga actcgtaccc gagcctccgc gagagccggt cgaacgccgc gaggtccttc
     1621 gggaaacacg agccgccgta gccgagcccg gccgtgaggt gcgcgcggcc gatccgaggg
     1681 tcggcgccca tgatgtcggc gaccttcagc acgtctgcgc cggccagctc gcagatccgc
     1741 gcgagcgcgt tcgcatagga gaccttcagc gccaggaacg cgttggacgc gtgcttcgcg
     1801 atctctgcgg tcgcgatgtc ggtctcgatc cacgtcgcgc cggcctcgac gaacggctcg
     1861 tagagctgcc gcatcgcgtc gcgggcctcc gccgagctcg tcccgaccag gatcctcccc
     1921 ggccgcagcg agtcctcgac cgcgctgccc tcgcgcagga actcggggtt gcacacgacc
     1981 tggaactcga gatccggtcg aacccggcgc atcgtctcgc gcacccggtc ccccgtcccg
     2041 aacgggaccg tgctcttctc gaccacgacg agcggctccg acgcgtagcg cgcgacgtcc
     2101 tcggccgaac gctcgacggc ggccaggttc gcctcaccgt cgcgccgtgg gggcgtgccg
     2161 acgcagatga acacgaccgg tgcgcccgcg acggcctcgc cggtgtcgga cgtgaacgag
     2221 atgcgcttcg caaggatccc ttcgcgcagc agctcctcga ggcccggctc gaagaacggc
     2281 atcgcgccgc gctcgagcag cgctatcttc tcgtggtcga cgtcggtgac gacgacctcg
     2341 tgccccacgc gggcgaaggc gccggcggtc gggagcccca cgtgaccggc gccgacgatc
     2401 ccgaccttca ccggcccgtc tccgcggcgc ccggtctggc tccctcggtg aaccagcgga
     2461 ccgtcgcccg caccgactcc tcccatcgcg cctgcggctc gaacccgagc agctcccgcg
     2521 cgcgcgagat gtcggcgtgg gtcttccgaa cgtcgccggc gcgctgcggc tggcgctccg
     2581 gctcgatcca cgtcccgacg acgtcggaga cggcctggag cacctcgttc acggtcttgg
     2641 gatcgccgcc ggcgatgttg atgacggccc cgtcgacgcg ctcgtcg
//
LOCUS       DASZOB010000460         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_171495, whole genome shotgun sequence.
ACCESSION   DASZOB010000460 DASZOB010000000
VERSION     DASZOB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171495"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VG709_07195"
     CDS             <1..232
                     /locus_tag="VG709_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32897.1"
                     /translation="SYIDEVAGMRSNGGNGGGRGGAAGGGTGAPGETGPTAAGRPGAP
                     QRDEPGFGMGYPPRPGEAEGGTSGQRPEEPGG"
     gene            225..764
                     /gene="atpH"
                     /locus_tag="VG709_07200"
     CDS             225..764
                     /gene="atpH"
                     /locus_tag="VG709_07200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HWC32898.1"
                     /translation="MARDAATQGYAEALFQIAQAEGALDAVENELFDFARTIEREPQL
                     RQALTDPALPNERKQALLNELLGERANPHTTRLLGFVVEQGRARDLASIVDELVRLAA
                     ERRNSVVAEVRTAVPLDDERRRRLEGALGRATGRRVELKTVVDPSVVGGVVARVGDQV
                     IDGSVRRRLELARERLSEV"
     gene            766..>1968
                     /gene="atpA"
                     /locus_tag="VG709_07205"
     CDS             766..>1968
                     /gene="atpA"
                     /locus_tag="VG709_07205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWC32899.1"
                     /translation="MAEQLTIRPDEIAEALRRHVESFRPETEREEVGRVAETGDGIAR
                     VVGLPGTMANELLEFPEEIYGLAFNLEEDEIGCVLFGDAERIEEGDAVKRTGRILSVP
                     VGDALLGRVVDPLGRPLDGKGPIATTEQRALEVQAPSVVERQPVKEPLQTGIKAVDAM
                     TPIGRGQRELIIGARQTGKTAIAIDAIINQRDNWASGDPKRQVKCVYVAIGQKDSTVR
                     EVEEALRANGALDYTVIVSAPASLPASFKFIAPYGGSAIGQHWMYNGQHALIVFDDLS
                     KQAEAYRQVSLLLRRPAGREAYPGDVFYLHSRLLERCAKLSDDLGAGSLTGLPFVETK
                     GGDVSAYIPTNVISITDGQIYLETDLFYSGVRPAINVGISVSRVGGNAQIKAMKQVAG
                     RLRLDLAQY"
BASE COUNT          312 a          656 c          741 g          259 t
ORIGIN      
        1 ctcctatatc gacgaggtcg ctgggatgcg ctcgaacggc ggcaacggcg gcggacgagg
       61 cggagctgca ggtggcggta cgggagcgcc gggggaaacg ggtccgacgg ccgccggacg
      121 tccgggagca ccccagcgcg acgagccggg cttcggaatg ggatacccgc cgcgcccggg
      181 agaggccgag ggcgggacgt cggggcagcg gccggaggag ccgggtggct agggacgccg
      241 cgacgcaggg ctacgccgag gcgctcttcc agatcgcaca ggccgagggc gcgctcgacg
      301 ccgtcgagaa cgagctgttc gacttcgcgc ggacgatcga gcgggagccg cagctgcggc
      361 aggcgctcac cgatccggcg cttccgaacg agcggaagca ggcgctgctg aacgagctcc
      421 tcggcgagcg cgcgaacccg cacacgacgc ggctgctcgg cttcgtcgtc gagcagggcc
      481 gcgcccggga cctcgcctcg atcgtggacg agctcgtgcg gctcgccgcg gagcgtcgaa
      541 acagcgtcgt ggccgaggtg cggacggccg taccgttgga cgacgagcgc cggcgccggc
      601 tcgagggcgc gctgggtcgg gcgaccggaa ggcgggtaga gctgaagacg gtggtggatc
      661 catcggtcgt gggcggcgtc gtcgcgcgcg tcggcgatca ggtgatcgac ggatcggtcc
      721 gccgccggtt ggagctggcg agagagcgac tgagcgaggt gtgacgtggc ggaacagctg
      781 acgatccggc ccgacgagat cgcggaggcc ctgaggcggc acgttgagtc gtttcgcccc
      841 gagacggaac gagaggaggt cgggcgcgtc gcggagaccg gcgacggcat cgcgcgcgtg
      901 gtcggtctcc ccggcaccat ggccaacgag ctcctcgagt tcccggagga gatctacggg
      961 ctcgcgttca acctcgagga ggacgagatc ggatgcgtcc tcttcggcga cgccgagcgc
     1021 atcgaggagg gcgacgccgt caagcggacc ggccggatcc tgtccgtgcc ggtcggcgac
     1081 gcgctgctcg gacgggtcgt cgacccgctc ggccgtccgc tcgacggcaa gggtcccatc
     1141 gcgaccacgg agcagcgggc gctcgaggtg caggcgccgt cggtcgtcga gcgccagccc
     1201 gtgaaggagc cgctccagac ggggatcaag gccgtcgacg cgatgacccc gatcggccgc
     1261 ggtcagcgcg agctgatcat cggcgcccgc cagaccggca agaccgcgat cgcgatcgac
     1321 gcgatcatca accagcgcga caactgggcg agcggcgacc cgaagcggca ggtgaagtgc
     1381 gtgtacgtcg cgatcggcca gaaggactcg acggtgcgcg aggtcgagga ggcgctgcgc
     1441 gcgaacggcg cgctcgacta cacggtgatc gtgagcgccc ccgcctcgct gccggcgtcg
     1501 ttcaagttca tcgctcccta cggtggatcg gcgatcgggc agcactggat gtacaacggc
     1561 cagcacgcgc tgatcgtctt cgacgacctc tcgaagcagg ccgaggcgta ccgtcaggtc
     1621 tcgctgctgc tccgccgtcc cgccgggcgc gaggcgtacc cgggcgacgt tttctacctc
     1681 cactcccggc tgctcgagcg ctgcgccaag ctctccgacg acctcggtgc cgggtcgttg
     1741 accggtctgc cgttcgtcga gaccaagggc ggcgacgtct ccgcgtacat cccgaccaac
     1801 gtgatctcga tcaccgacgg gcagatctac ctcgagaccg acctcttcta ctcgggcgtg
     1861 cgtccggcca tcaacgtcgg catctcggtg tcgcgcgtcg gcggcaacgc gcagatcaag
     1921 gcgatgaagc aggtcgccgg acgcctccgc ctcgacctcg cgcagtac
//
LOCUS       DASZOB010000461         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_136284, whole genome shotgun sequence.
ACCESSION   DASZOB010000461 DASZOB010000000
VERSION     DASZOB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136284"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VG709_07210"
     CDS             complement(<1..394)
                     /locus_tag="VG709_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWC32900.1"
                     /translation="MRQSPLRHVLLVVANIVGLAAFLWPFVLPAAVGARGAHEADAPV
                     VFVLLLACLGALLFVELGRGGLGPKAVALIGVLGAAMVALRLPGFIAGFSAMFIVVLV
                     AGNAFGPGFGFVLGAVGTFASGLFIGGLG"
     gene            complement(391..1977)
                     /locus_tag="VG709_07215"
     CDS             complement(391..1977)
                     /locus_tag="VG709_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWC32901.1"
                     /translation="MTDAVVFDNVTFQYMERAEYALREVTLSIPEGAFTLVCGATGAG
                     KSTLLRAINGLVPHFPGGGFSGHVRVEGRDTLEFPPRKLADVVAFVPQDPGASFVVDR
                     VEDELAYAMENLAIDPARMRRRVEETLDLLDIERLRTRSVRTLSGGERQRVAIASALT
                     PGAKVLLLDEPTSQLDPQAAEDVLAALQRLVHDLGLTVVIAEHRLDRVAGFVDLAIGC
                     HGKGRVEIGDPAAVLERLQTGPPVSRLGRMLGWRPVPLTVRDARRMAAGRRAAPARSP
                     RPRVQLGEVLLEARDLTASYDGTTVLRDVTLSLRAGEIVALMGRNGAGKTTLLRCLAG
                     VQHSDAGAVVVEGGSPRTGIDVALCPQTPEDVLFSDTVADEVRATLKAHGRLDDGSVE
                     SVLDGLGIGHLAERHPRDLSAGERLLAAVAAVVTTDARVLLLDEPTRGLDAETKERLS
                     KFLRAHAAGGRAVVFASHDVELVAELANRVVMLAGGEQIADGTPRAVLAESPVFAPQT
                     AKVLGGDFMTPDDVGTSVPG"
     gene            complement(1974..2789)
                     /locus_tag="VG709_07220"
     CDS             complement(1974..2789)
                     /locus_tag="VG709_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWC32902.1"
                     /translation="MDRQAETVAALIHPAGWIAWATCAALVTMSTTNPFYLLPVVAGS
                     WAIHAAHRRSGPFARSFRVFLIIGLVTIMTRTLLVFLVPSSVSAATVATAFLEGLRLA
                     VLLIVFGAFNSVADPFGVLRLAPRRFHEPALAAALALSIAPRTIAAVAQVRDAQRLRG
                     IRIKTWRSLPALAVPVLETGMEEAVTLAESMDARGHGGGPRSRYRPDRWTHPALAIVA
                     SALVAAFMFLYQAVAAAASLTVQTSPVQWPEASPVLVGAVLLLAVPAFVRPRP"
     gene            complement(3081..>3370)
                     /locus_tag="VG709_07225"
     CDS             complement(3081..>3370)
                     /locus_tag="VG709_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32903.1"
                     /translation="RRRLLRGIPRVLGLLARERFRRMVVVVYRSRLAPRVRWGRRGMV
                     VGERQRRQLPSGAPANLVLVGVPGGRGAVSGQPPTQAARRSASGCRPRAGT"
BASE COUNT          603 a         1237 c         1123 g          407 t
ORIGIN      
        1 gcccgagccc accgatgaac agacccgacg cgaacgtgcc gaccgcgccc aggacgaagc
       61 cgaagccggg gccgaacgcg ttccccgcaa ccagcaccac gatgaacatg gccgagaacc
      121 ccgcgatgaa ccccggcaac cgcaacgcca ccatcgccgc ccccagcacg ccgatcaatg
      181 cgaccgcctt cggtccgagt ccgccccggc cgagctcgac gaacaggagc gcgcccagac
      241 acgcgaggag aaggacgaag acgaccggag cgtcggcctc gtgcgcgccg cgcgcgccta
      301 ccgccgcggg caggacgaac ggccacaaga aagcagccag gcccacgatg ttggcgacaa
      361 caagcaagac gtggcgcaga ggtgactgcc tcatccgggc accgaagtac ccacgtcatc
      421 gggcgtcatg aagtcgcctc ccaacacctt cgcggtttgg ggcgcgaaca ccggcgactc
      481 cgccaacact gcacgcggcg tcccgtccgc gatctgttca ccgcccgcga gcatgacgac
      541 gcgattcgcg agctcggcca cgagctccac gtcgtgcgac gcgaacacga ccgcgcggcc
      601 gccggcggcg tgggcgcgca ggaacttcga caaacgttcc ttcgtctctg catcgagacc
      661 tcgggtcggt tcgtccagga ggagcacgcg cgcgtcggtc gtaaccaccg ctgcaaccgc
      721 ggcaagcagc cgctcgcccg ccgacagatc ccgcggatga cgctcggcga gatggccgat
      781 gccgagtccg tcgaggaccg actcgacgga gccatcgtcg aggcggccgt gagccttcaa
      841 cgtcgcccga acctcgtccg cgaccgtgtc cgagaacagg acgtcctccg gcgtctgggg
      901 acacaacgct acgtcgatgc ccgttcgcgg cgatcctcct tcaaccacga ccgctccggc
      961 gtccgagtgc tgcacgcccg cgaggcaacg gagcaacgtg gtcttgcccg ctccgttgcg
     1021 acccatcaac gcgacgatct ctccagcgcg cagcgacagc gtcacgtcgc gcaggacggt
     1081 cgtgccgtca tacgacgcag tcaggtcgcg cgcctccagg agcacctcgc ccaactgaac
     1141 gcgcgggcgc ggtgaacggg caggagcggc gcgtcgacct gccgccatcc gccgggcgtc
     1201 gcggaccgtc agcgggacgg ggcgccagcc gagcatccgc cccaaccggg agacgggggg
     1261 gccggtctgc agccgctcca agaccgccgc cggatcaccg atctcgacgc ggcctttccc
     1321 atggcatccg atcgcgaggt cgacgaatcc cgcgacccgg tcgagtcggt gctcggctat
     1381 cacgacagtc agaccgaggt cgtggaccaa acgctgcaga gccgcgagaa cgtcctccgc
     1441 ggcctgcggg tctagctgac tcgtcggctc gtccaggaga aggaccttcg cccccggtgt
     1501 gagagcagaa gcgatggcaa cgcgctggcg ctcgcctccg gacaaggtcc tgacacttcg
     1561 cgtccgcaac cgctctatgt ccagcaagtc cagcgtctct tcgacccgtc gtcgcatacg
     1621 cgcagggtcg atcgccaggt tctccatcgc gtacgcgagc tcgtcctcca cgcgatcgac
     1681 gacgaacgac gcgccggggt cctgcggcac gaacgcaacc acgtcggcga gcttccgggg
     1741 ggggaactcg agcgtgtcgc ggccttcgac ccggacgtgt ccggaaaacc ccccgccggg
     1801 gaaatgcgga acgagcccgt tgatggcgcg aagcagcgtc gacttgccgg cgccggtcgc
     1861 gccgcacacc aatgtgaacg caccttcggg gatcgacagc gtcacctctc tgagcgcgta
     1921 ctcggcgcgc tccatgtatt ggaacgtgac gttgtcgaaa acaacggcgt ccgtcacggg
     1981 cgcggcctca cgaaggcggg caccgcgagc agtaacaccg cacccacaag gaccggcgat
     2041 gcctcgggcc actgcaccgg agaggtctga acggtcagag acgccgcggc ggcgacagcc
     2101 tgatacagga acatgaacgc ggcgacgagc gcgctcgcga cgatggccag agcgggatgg
     2161 gtccagcggt cggggcgata gcgcgaccga ggaccacccc cgtgcccgcg cgcgtccatg
     2221 ctctcggcga gcgtgacggc ctcttccata ccggtctcca gaaccggcac cgcaagagcc
     2281 ggtagtgaac gccacgtctt gatgcggatc ccgcgcagcc gttgcgcgtc gcgaacctgc
     2341 gccactgcgg cgatcgtgcg cggggcgatc gagagcgcga gcgccgcggc gagcgccggc
     2401 tcgtggaacc gcctgggagc caaacgcaac accccgaacg ggtcggcgac cgagttgaag
     2461 gcgccgaaca cgatgaggag gaccgccaac cgaagcccct cgaggaacgc cgtcgccacc
     2521 gtcgcggcgc tcacggacga cggcaccagg aacaccaaca gggtccgggt catgatcgtg
     2581 acgagcccga tgatcaagaa gactcggaaa gaccgcgcaa agggaccgct gcgtcgatgc
     2641 gcggcgtgta tggcccacga acccgcgaca acaggcagga ggtagaacgg gttcgtggtc
     2701 gacatcgtca cgagcgccgc acatgtcgcc cacgcgatcc agcccgccgg atggatcaag
     2761 gccgcaaccg tctccgcttg gcgatccata ctccggcccc tccaaggagt cctgccgccg
     2821 cgagcgcggc taccccggcc ggcggaggcc caccgcggcc gcctggagga ggagcgccgg
     2881 agctcggctc gctcccgatc gccgcgtcga ccggctcctc cggaggacgc gccatgcggc
     2941 gactgcgccg cggcgtcccc ctagctcgcc tggcacgccg ccgctgcgcg tccgcctcct
     3001 gttgccggcg tccggagcgc tcccgtgcct tggacggaga tacgggcgcg ctcgcagcgt
     3061 cccgcgccga cgaagagggt tcaggttccc gctcggggtc ggcagccgga cgcggagcgg
     3121 cgcgcggctt gggtcggcgg ctggccggag acggcgccgc ggccgccggg cacaccgacg
     3181 agaacgaggt tggcgggggc gccggatggg agctgccgtc gttgccgctc ccccacgacc
     3241 atccctcgac gtccccatcg gacacgcggg gcgagccggc tccggtagac gaccacgacc
     3301 atccgccgga accgttcccg cgccagtagc cccagaactc ggggtattcc tcgaagcagt
     3361 cgtcgccgct
//
LOCUS       DASZOB010000462         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_136354, whole genome shotgun sequence.
ACCESSION   DASZOB010000462 DASZOB010000000
VERSION     DASZOB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136354"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VG709_07230"
     CDS             complement(<1..358)
                     /locus_tag="VG709_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWC32904.1"
                     /translation="MLKRGMVVAVVGASLLLSACGGGDAGGDAGGETGGDTAGGGATL
                     TMVDNAFEPADATFTSGEEIELVNDGEALHNLTVEGSGIDEDVEAGQSSTVAVDLDPG
                     EYAMFCEYHRAAGMEGT"
     gene            complement(450..>1930)
                     /locus_tag="VG709_07235"
     CDS             complement(450..>1930)
                     /locus_tag="VG709_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWC32905.1"
                     /translation="RVVRVVLENQFSPWVSAKDVILEMLRRLTVKGGLGKAFVFDGPG
                     VATLSVAERATICNMIQELGATAGIFPADDRTREWLSAQQRAEDFRELANDDGAPFDE
                     EMVIDLAELEPLIAKPRNPDNVVPVREIAGTKVAQVCVGSSVNSGYADLAIPAYVVKE
                     GGGVDEWLDMTVSPGSRQILDAITRTGVLADYVQSGARLLEPACGPCVGMGQAPPSGR
                     PSVRTFNRNFQGRSGTVDDYVYLTSPATAGATALKGVITDPRELDMEPPRVEEPPVHV
                     NDSMLIFPPQPEDARKTEIWRGPNIKPPPMPPELPDALEGRVLIVLPDNISTGSMSPD
                     GVIVMAERSNIPAIAEYCFRKEDPQFVSRAKEWGGGYIVAGDNYGQGSSREHAALAPL
                     QLGVRAVFAKTIHRIHRRNLINNGIVPVLIDDEVYAKAVLGDRWRLPRIREELRSGSP
                     TFTLDLDGSTTQVSNDLGRHEREQILAGGLLRYLKERGGDAA"
BASE COUNT          269 a          691 c          657 g          313 t
ORIGIN      
        1 gcgtcccttc catccccgcg gcgcggtggt actcgcagaa catcgcgtac tcgccggggt
       61 cgaggtcgac cgcgacggtc gagctctggc cggcctcgac gtcctcgtcg atccccgatc
      121 cctcgaccgt caggttgtgg agcgcctcgc cgtcgttcac gagctcgatc tcctcgcccg
      181 acgtgaacgt cgcatcggcc ggttcgaacg cgttgtcgac catcgtgagc gtggcgccgc
      241 ctccggccgt gtcgccgccc gtctcacccc cggcgtcccc gccagcgtct ccacctccgc
      301 acgcggaaag gagcagcgac gctccgacca cggcgaccac catccctcgc ttgagcacga
      361 tcaccctcct tcgttcggtg tgagggaagt gtccgacacg aggcggtaac caccgcccaa
      421 gacgcggtta agggagcgtg cgggtgaccc taggcggcgt cgccgccgcg ttccttgagg
      481 taccggagaa ggccgccggc gaggatctgc tcgcgctcgt ggcgcccgag atcgttgctg
      541 acctgggtcg tgctgccgtc gaggtcgagc gtgaacgtcg ggctgccgga ccggagctcc
      601 tctcggatac gcgggagccg ccagcggtcc ccgagcaccg cctttgcgta cacctcgtcg
      661 tcgatgagca ccgggacgat gccgttgttg atgaggttgc ggcggtggat ccggtggatc
      721 gtcttggcga acaccgcgcg aacgccgagc tggagcggcg cgagcgcggc gtgctcgcgc
      781 gacgatccct ggccgtagtt gtcgccggcg acgatgtagc cgccgcccca ctccttcgcg
      841 cggctcacga actgcgggtc ctctttccgg aagcagtact cggcgatcgc cgggatgttc
      901 gagcgctcgg ccatcacgat cacgccgtcc ggcgacatcg accccgtcga gatgttgtcg
      961 ggaaggacga tgaggacccg cccctcgagc gcgtcgggga gctccggcgg catcggcggc
     1021 ggcttgatgt tcgggccgcg ccagatctcg gtcttccgcg catcctccgg ctgcgggggg
     1081 aagatcagca tcgagtcgtt gacgtgcacg ggtggctcct cgacgcgtgg cggctccatg
     1141 tcgagctcgc gaggatccgt gatcacgccc ttcagcgcgg tcgcgccggc cgtggcgggg
     1201 gacgtgaggt agacgtagtc gtcgacggtc ccggaccgcc cctggaagtt gcggttgaac
     1261 gtccggaccg acggccggcc cgatggcggc gcctgcccca tcccgacgca cggcccgcac
     1321 gccggctcga ggagccttgc cccggattgg acgtagtcgg cgaggacgcc ggtcctcgtg
     1381 atcgcgtcga ggatctggcg cgaccccggc gacaccgtca tgtcgagcca ctcgtcgacg
     1441 ccgcccccct ccttgacgac gtaggccggg atcgcgaggt cggcgtagcc ggagttcacc
     1501 gacgagccga cgcagacctg tgcgaccttc gtccctgcga tctcgcgcac cggcacgacg
     1561 ttgtcggggt tccgcggctt cgcgatcagc ggctcgagct cggcgaggtc gatcaccatc
     1621 tcctcgtcga agggcgcgcc gtcgtcgttc gcgagctcgc ggaaatcctc cgcgcgttgc
     1681 tgcgccgaca gccactcacg cgtccgatcg tcggccggga agatcccggc ggtcgcgccg
     1741 agctcctgga tcatgttgca gatcgtcgcg cgctcggcga ccgacagcgt agcgacgccg
     1801 gggccgtcga agacgaacgc cttgccgagc ccgcccttca ccgtgagccg ccgcagcatc
     1861 tcgaggatca cgtccttcgc cgacacccac ggcgagaact ggttctcgag tacgacgcgc
     1921 acgacccgcg
//
LOCUS       DASZOB010000463         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_136461, whole genome shotgun sequence.
ACCESSION   DASZOB010000463 DASZOB010000000
VERSION     DASZOB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136461"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VG709_07240"
     CDS             complement(<1..1157)
                     /locus_tag="VG709_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWC32906.1"
                     /translation="MSGSGHITAEPPPRPERVAAAEPGRWRKYALAAGFLAPAAFFLG
                     IWIVYPTVYTIARSFFGREGFGDPVWFDNYRTLFTSDVLLTAIKNNAIWVAVVPALVT
                     AIGLVFAVLTERVGWAVAFKTAVFMPMAISAFAAGITWRLVYIQNPEQGALNAAIGSV
                     RDVVKPAGVLSTARPSSEDLGGTPAGGIELSDAVSPGEVAQLGLTAIPPDDVPEDAEE
                     AAVPEPLQGGITGVVWRDFKPGGGTPGEVESEELGLPGVTVELRDARGEAIETATTAE
                     NGTFEFPDVGEGEYRVAIGPDTFAQRFGGVTWLGEKLVTPSIIIAYLWIWAGFAMVVI
                     AAGLSAIPRDVLEAARTDGASEWQVFRRVTAPLLAPVLTVVFITMLINVLKV"
     gene            complement(1167..2474)
                     /locus_tag="VG709_07245"
     CDS             complement(1167..2474)
                     /locus_tag="VG709_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWC32907.1"
                     /translation="MRRWFVLLAVFAGLVPAFAAGCGGDDGDEAAGTDTATEGGEAVS
                     GSVSVMSTWSGPEQETFQAVIDGFTEQNPDVDVRYDSAGDALPTVLSTAVEGGNPPTI
                     AVLAQPGLIQGYADRGAIKPLDFAEDVISENFGESVVETGRFNDQLYSFLFKADNKST
                     VWYNVQAFESAGVEPPETWEDLQGAADTLSASGIPAWSFGAADGWVLTDFFENIYLRQ
                     AGADMYDQLAEHQIPWTDESVKEALRTMGEIFENKDNIAGNALQIDFPTSVSAVFSEN
                     PEAAMIAEGDFVPGVVESPLEPETGYNVFSFPSIDDSPPVVVGGGDTVSMFTDSEAAQ
                     ALVEFLATPEAAEVWAQQGGFATLAKDVDPNIYPDEITRTTAGALSEAETFRFDLSDL
                     QPAEFGGTVGQGLFKLFQDFYANPSDVDGIAQQMERAAARAYG"
BASE COUNT          404 a          895 c          890 g          394 t
ORIGIN      
        1 accttgagga cgttgatcag catcgtgatg aagacgaccg tgaggacggg tgccaggagc
       61 ggcgccgtga cgcgcctgaa gacctgccac tcggacgcgc cgtcggtgcg ggcagcctcg
      121 aggacgtcgc gcgggatcgc cgagaggccg gccgcgatca cgaccatcgc gaagcccgcc
      181 cagatccaga ggtaggcgat gatgatcgac ggggtgacga gcttctcacc gagccacgtc
      241 acgccgccga agcgctgcgc gaacgtgtcg gggccgatgg cgacgcggta ctcaccctcc
      301 ccgacgtcgg ggaactcgaa ggtgccgttc tcggcggtcg tcgccgtctc gatcgcctcg
      361 ccccgggcgt cgcggagctc gacggtcacg ccgggaagcc cgagctcttc gctttcgacc
      421 tcgccgggcg taccgccgcc cggcttgaag tcgcgccaga cgacaccggt gatcccgccc
      481 tggagcggct ccggaaccgc cgcctcctcc gcgtcctcgg ggacgtcgtc gggagggatc
      541 gcggtcaggc cgagctgcgc cacctcgccg ggcgacaccg cgtcggaaag ctcgatcccg
      601 ccggccggag tgccgccgag atcctcgctc gacgggcgtg cggtcgagag cacgccggcc
      661 ggcttgacga cgtcgcgaac cgacccgatc gccgcgttca gcgcaccttg ctcggggttc
      721 tggatgtaga cgaggcgcca cgtgatcccc gccgcgaacg cggagatcgc catcggcatg
      781 aagacggccg tcttgaacgc aaccgcccat cccacccgct ccgtgagaac ggcgaacacg
      841 agcccgatcg cggtcacgag cgccgggacg acggcgaccc agatcgcgtt gttctttatc
      901 gcggtgagga gaacgtcgct cgtgaacagc gtccggtagt tgtcgaacca gacggggtcg
      961 ccgaacccct cgcgcccgaa gaagctccgg gcgatcgtgt acaccgtcgg gtagacgatc
     1021 cagatgccga ggaagaaggc ggcgggcgcg aggaacccgg ccgccagcgc gtacttgcgc
     1081 cagcgaccgg gctccgccgc ggctacccgt tcgggccgtg gcggcggctc agcggtgatg
     1141 tgcccggagc cgctcacgct cgtcggctag ccgtacgccc tcgcggccgc ccgctccatc
     1201 tgctgcgcga tcccgtcgac gtccgagggg ttcgcgtaga agtcctggaa cagcttgaag
     1261 aggccctggc cgaccgtgcc gccgaactcg gcaggctgga ggtcggagag gtcgaagcgg
     1321 aaggtctccg cctccgagag cgcgccggcg gtcgtccgcg tgatctcgtc cgggtagatg
     1381 ttcgggtcga cgtccttcgc gagcgtcgcg aacccgccct gctgggccca gacctcggcc
     1441 gcctccggcg tcgcgaggaa ctcgacgagc gcctgcgcgg cctccgaatc cgtgaacatc
     1501 gagaccgtgt cgccgccgcc gacgacgacc gggggcgagt cgtcgatgga cgggaacgag
     1561 aagacgttgt accccgtctc gggctcgagc ggcgactcga cgacgcccgg cacgaagtcg
     1621 ccctcggcga tcattgccgc ctccgggttc tcggagaaga cggccgagac ggaggtcggg
     1681 aagtcgatct ggagggcgtt acccgcgatg ttgtccttgt tctcgaagat ctcgcccatc
     1741 gtccggagcg cttccttcac cgactcgtcc gtccagggga tctggtgctc ggcgagctgg
     1801 tcgtacatgt ccgcgccggc ctgccggagg tagatgttct cgaagaagtc ggtcaggacc
     1861 cagccgtccg ccgccccgaa cgaccacgcg gggatccccg aagcgctgag cgtgtcggcc
     1921 gccccctgga ggtcctccca cgtctccggc ggctcgacgc cggcgctctc gaacgcctgg
     1981 acgttgtacc agacggtcga cttgttgtcc gccttgaaga ggaacgaata gagctggtcg
     2041 ttgaaccggc cggtctcgac cacggattcg ccgaagttct cgctgataac gtcctcggcg
     2101 aagtcgagtg gcttgatcgc cccgcggtcc gcgtaaccct ggatcaggcc gggctgggcc
     2161 aggaccgcga tcgtcggcgg attcccgccc tccaccgccg tcgagagcac ggttgggagc
     2221 gcatcgccgg cggagtcgta ccggacgtcg acgtccgggt tttgttcggt gaagccgtcg
     2281 atgacggctt ggaacgtttc ctgctcgggg ccggaccagg tgctcatgac cgagactgag
     2341 ccggatacgg cctcgccacc ctccgtcgca gtatcggtgc cggcggcctc gtctccgtcg
     2401 tcgcctccgc agccggcggc gaacgccggc acgagcccgg cgaagaccgc gagtagaacg
     2461 aaccaacgcc tcatcaagct ccctccctgg aagtcgtgtc gtagattcct tccccgctct
     2521 cggggtcgaa gaagtgcagc gagcgtgtgt cgacggccac ctcggcggct cggacgtcag
     2581 gac
//
LOCUS       DASZOB010000464         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_187431, whole genome shotgun sequence.
ACCESSION   DASZOB010000464 DASZOB010000000
VERSION     DASZOB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187431"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..473)
                     /locus_tag="VG709_07250"
     CDS             complement(174..473)
                     /locus_tag="VG709_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32908.1"
                     /translation="MSDPRGTIVLEMGEIASVVTLDAWRRHRDASGPGEERLDRAVDR
                     LERVLVTRGWDRLAPDWVLTELLAIQGCLSLGLLQAAVLRTERLADRAEPRRARR"
     gene            506..1144
                     /locus_tag="VG709_07255"
     CDS             506..1144
                     /locus_tag="VG709_07255"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HWC32909.1"
                     /translation="MSSTAVAVAEILAALSYGERLAQRRADDTVVLAPDESARKQQQH
                     VARRELENATLIGARVRELGSVESEERFRPFFDAFFARSVPSDWLEAQTFHYLGDALV
                     RDFADVLVPLLDPVSAEVVRRALADRDEQDAFALDEITRGTSDDPEAAERIAAYARRI
                     VGEALTQARRALDETEALRSLLGDEEGEKRLLLDLLQRHRQRLERMGIEIVE"
     gene            1155..>2592
                     /locus_tag="VG709_07260"
     CDS             1155..>2592
                     /locus_tag="VG709_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWC32910.1"
                     /translation="MERPEPVRDLDWDPKRARELGERAVDLWEELLTRLRDLPVGRRA
                     AAPEVRDAIALEIPPEPMPLEGLIAYLHDVMFERSMYPGHPGFMAYITGAGTVPGAAA
                     DLLAAALNQNLGGWRLSPAATEIEMHLTKWFADRFGLPDTAGGILVSGGAMAGYISLK
                     VARDRKAGWDVRTGGVSVGPPLAIYASAEVHSINNRAADMLGLGRDAVRLVPVDARYR
                     MDVAALRSAIEEDRRAGVRPIAVVASAGTVATGAIDPLREIVDVCRDEDLWFPVDAAY
                     GGPAVLAPELRPLFDGIERADSIAFDPHKWMYTPHSGGCVLMREFQHLPDSFSISAHA
                     AYVHEDKERTGTGVDGIELGPQFSRGFQALKIWMSLLAHGEDAYARRIAHDAELARYL
                     AAEADRRPEFESVAPVTLSIACFRYVPPGLPNGEGREQYLDALNERLMTELQLDGRVY
                     PSNAVLGGRFVLRACIVNFRTEADEIDLL"
BASE COUNT          375 a          909 c          939 g          369 t
ORIGIN      
        1 atccgggcga ggcgccggaa ggtgcctcag aagggggcag aacccacgct cggtctgccg
       61 cgggaggcat actcgggtgc ccgtgccgca aggggcgcgg ccgcctggga ccaggaccgt
      121 gatggagagc gcatggtagc tgcccccttc gccgtgcgag ggccgcggta gcgctagcgg
      181 cgggcccggc ggggctcggc gcggtccgcg agccgctcgg tccggagcac cgccgcctgg
      241 agcagcccga gcgacaggca cccctggatc gcgagcagct cggtcagcac ccagtcgggc
      301 gcgagccggt cccagccgcg ggtgaccagc acgcgctcga gccggtcgac ggcccgatcg
      361 agccgttcct cgccgggtcc ggacgcgtcc cgatgcctgc gccaggcgtc gagcgtgacg
      421 acgctcgcga tctcgcccat ctcgaggacg atcgtgccac gggggtctga catttcctcg
      481 taggcgaccc catacaattt cggacgtgtc gagcaccgcc gtggcggtgg cggagatcct
      541 cgcggcgctc tcctacggag aacggctcgc tcagcgtcgc gccgacgaca ccgtcgtcct
      601 cgcgcccgac gagagcgccc gcaagcagca gcagcacgtc gcgcgccgcg agctcgagaa
      661 cgcgacgctg atcggcgccc gcgtccgcga gctcggatcc gtggagtccg aggagcgctt
      721 ccgcccgttc ttcgacgcgt tcttcgcgcg gagcgtgccg tcggactggc tcgaggccca
      781 gaccttccac tacctggggg acgcgctcgt ccgggacttc gccgacgtcc tggtgccgct
      841 cctcgacccg gtctccgccg aggtcgtccg gcgggcgctg gccgaccgcg acgagcagga
      901 cgcgttcgcg ctcgacgaga tcacgcgcgg cacgagcgac gatcccgagg cggcggagcg
      961 gatcgcggcg tacgcgcggc gcatcgtcgg cgaggcgctg acgcaggcgc ggcgcgcgct
     1021 cgacgagacg gaggcgctgc ggagtctcct cggcgacgag gagggagaaa agcggctgct
     1081 gctcgacctc ctccagcgcc accgacagcg tctcgaacgc atggggatcg agatcgtcga
     1141 atagggtgac gccgatggag cggccggagc cggtacgcga cctcgattgg gacccgaagc
     1201 gcgcgcgtga gctcggcgag cgcgcggtgg acctgtggga ggagctccta acgaggctgc
     1261 gtgatcttcc ggtcgggcga cgtgccgccg cacccgaggt cagagacgcg atcgcgctcg
     1321 agatccctcc ggagccgatg ccgctcgaag gcctcatcgc gtacctgcac gacgtgatgt
     1381 tcgagcgctc gatgtacccg gggcatcccg ggttcatggc gtacatcacc ggcgcgggga
     1441 ccgttccggg agcggcggcc gacctgctcg ccgcggcgct aaaccagaac ctcggtggct
     1501 ggcggctgtc gccggccgcg accgagatcg agatgcacct cacgaagtgg ttcgcggatc
     1561 ggttcgggct cccggacacg gccggcggga tcctcgtctc cggcggagcg atggccgggt
     1621 acatctcgct gaaggtcgcc cgcgaccgca aggccgggtg ggacgttcgg acaggcggcg
     1681 tgtcggtggg tccgccgctc gcgatctacg cctccgcaga ggtgcacagc atcaacaatc
     1741 gcgccgccga catgctcggg ctcggacgtg acgccgtccg actcgtccct gtggacgccc
     1801 ggtaccgaat ggacgtcgcg gcgctccggt cggcgatcga ggaggaccgc cgagccggcg
     1861 tccgtccgat cgcggtcgtc gcgtcggcgg ggaccgtggc gacgggtgcg atcgacccgc
     1921 ttcgtgagat cgtcgacgtc tgccgcgacg aggatctctg gttccccgtc gacgccgcgt
     1981 acggcggacc cgccgtcctc gcgccggagc ttcgcccgct gttcgacggc atcgagcgcg
     2041 cggactcgat cgcgttcgac ccacacaagt ggatgtacac gccgcattcg ggcggatgcg
     2101 tgctgatgcg cgagttccag catctccccg acagcttctc gatcagcgcg cacgccgcgt
     2161 acgtgcatga ggacaaggag cggacgggga cgggcgtcga cggcatcgag ctcggcccgc
     2221 agttctctcg cggcttccag gcgctgaaga tatggatgtc gctgctcgcg cacggcgagg
     2281 acgcctacgc gcgccgcatc gctcacgatg ccgagctcgc ccgctacctc gcggctgaag
     2341 cggaccgccg cccggagttc gaatcggtgg cgccggtcac gctctcgatc gcgtgcttcc
     2401 gctacgtgcc gcccggcctg ccgaacggcg agggacgcga gcagtacctc gacgcgctca
     2461 acgagcggct catgacggag ctgcagctcg acggccgcgt ttacccgtcg aacgccgtgc
     2521 tgggcgggcg gttcgtactc cgggcctgca tcgtgaactt ccgcaccgag gccgacgaga
     2581 tcgatctctt gc
//
LOCUS       DASZOB010000465         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_136491, whole genome shotgun sequence.
ACCESSION   DASZOB010000465 DASZOB010000000
VERSION     DASZOB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136491"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VG709_07265"
     CDS             complement(<1..1259)
                     /locus_tag="VG709_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC32911.1"
                     /translation="MPGSAAAPRVVVVIVDGLPVELLERELPRLPFLRERLPYGGEAI
                     SCFPSTTGPAYFPFLAGATPGRANVPGIRWFDRTAKTRSRFPHRGLRSYVGPDVGKMR
                     TDTGVSTVFARHAWPVSSPVDKDSPKRRERSRDLVWALAHFLPTWAHADRRTAWKLGR
                     GLGTGRPIVFSVFPSVDEFGHVYGLADARGAEALRTIDRLLAEKLEAFPGEIVLSADH
                     GLTEPTTHLDLRALVEERVGPTLAFPLIGMPDPAAVVCESGNAMANVYLRGDGGWRER
                     PSVDRGRALARELAVLDGIDSVAIRGPARYSAELHTRDGVGELGFSDGTLWQRGPVFA
                     TEFEGASPREALAQSFSGRWPDAAFALASLFASERTGDLLVSAAVGYDLRAAREWPEH
                     RASHGALHRAHTLVPVRSSAPLPDPPLR"
     gene            1279..1998
                     /locus_tag="VG709_07270"
     CDS             1279..1998
                     /locus_tag="VG709_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWC32912.1"
                     /translation="MRPNGERRIVVVPTYQEREALPRFLSELEPTGFDALVVDDNSPD
                     GTGEWAEAFSREHPWLHVLRRAGKEGLGSAYRAGFAWCLARGYDVVGQMDCDLSHPPE
                     ALAGMRTALERADVVVGSRFTRGGRTIGWPWWRKAQSYAATVPARLLLRLPVADLTGG
                     FKLWQSEALRRIEVESTVSQGYVFQIETTYRAFRAGLRVVEVPIAFAERAAGESKMST
                     AIKLEGVGVVLRLARERWRPS"
     gene            1983..>2286
                     /locus_tag="VG709_07275"
     CDS             1983..>2286
                     /locus_tag="VG709_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32913.1"
                     /translation="MAPVLIPVRYARRAAVSAALVAATGTLALSANRHLSGDVYWLLA
                     AGREIATNGLTDTDPFLTLNHGREWQNQQWLTELVLYGLERLGGLRLVSLAYALVLG"
BASE COUNT          322 a          815 c          853 g          296 t
ORIGIN      
        1 cgaaggggtg ggtccgggag cggagctgac gagcgaacgg ggacgagcgt gtgcgcccgg
       61 tgcagcgccc cgtgggacgc ccggtgctcc ggccactcgc gggcagcgcg caggtcgtag
      121 ccgaccgcgg cggagacgag caggtcgccc gtccgctcgg acgcgaacag ggacgcgagc
      181 gcgaacgcgg cgtccggcca gcgccccgag aaggactgtg cgagcgcctc gcgcggcgac
      241 gctccctcga actcggtcgc gaacaccggc ccgcgctgcc acagcgtccc gtccgagaag
      301 ccgagctcgc cgacgccgtc gcgtgtatgg agctcggccg agtagcgcgc cgggccccgg
      361 atcgcgacgg agtcgatccc gtcgagcacg gccagctcac gcgcgagcgc gcggccccgg
      421 tcgacgctcg gacgctcgcg ccagcctccg tcgccgcgca ggtacacgtt ggccatcgcg
      481 ttccccgact cgcagacgac ggcggccggg tcgggcatgc cgatgagggg gaacgcgagg
      541 gtcggcccca cccgctcctc gacgagcgcg cgcaggtcga ggtgcgtcgt cggctcggtg
      601 agcccgtgat ccgcggagag gacgatctcg ccgggaaacg cctcgagctt ctcggcgagg
      661 aggcggtcga tcgtccgcaa cgcttcggcg ccccgcgcgt ccgcgagccc gtagacgtgc
      721 ccgaactcgt ccacggacgg gaacacggag aagacgatcg ggcgccccgt cccgaggccg
      781 cgcccgagct tccatgccgt ccggcggtcc gcgtgcgccc acgtaggcag gaagtgagcg
      841 agcgcccaga cgaggtctcg gcttcgctcg cgccgcttcg gggagtcctt gtccaccggc
      901 gacgagaccg gccacgcgtg gcgggcgaag acggtgctga cgccggtgtc cgtccgcatc
      961 ttcccgacgt ccgggcccac gtagcttcgc aggccgcggt gcggaaagcg gctgcgcgtc
     1021 ttcgcggtgc ggtcgaacca gcggataccc gggacgttgg cgcggccggg cgtcgcacca
     1081 gcgaggaacg ggaagtacgc cggccccgtc gtcgacggga agcacgagat cgcttctccg
     1141 ccgtacggga gtcgctcgcg caggaacgga agacgaggaa gctcgcgctc gagcagctcg
     1201 acgggaaggc cgtccacgat tacgacgacg acacgcgggg cggccgcgga tcccggcacg
     1261 gtggaagtat gagaggcgat gaggccgaac ggcgagcggc gtatcgtggt cgtcccgacc
     1321 taccaggagc gggaggcgct cccgcggttc ctctccgagc tcgagcccac ggggttcgac
     1381 gcgctcgtcg tggacgacaa ttcgcccgac ggcacgggcg agtgggcgga ggccttctcg
     1441 cgcgagcatc cgtggctcca cgtcctccgc cgcgccggca aggagggtct cgggtcggcc
     1501 taccgcgccg gcttcgcgtg gtgcctcgcg cgcggctacg acgtcgtcgg ccagatggac
     1561 tgcgacctct cgcacccgcc ggaggcgctc gcaggaatgc ggaccgcgct cgagcgggcc
     1621 gacgtcgtcg tcggctcgcg cttcacccgc ggcggcagga cgatcggatg gccgtggtgg
     1681 cggaaggcgc agagctacgc cgccaccgtt cccgcccgcc tcctcctgcg actgcccgtc
     1741 gcggacctga ccggcggctt caagctgtgg cagtcggagg cgctgcggcg aatcgaggtc
     1801 gaatcgacgg tctcgcaggg gtacgtcttc cagatcgaga cgacgtaccg tgcgttccgc
     1861 gcgggactcc gcgtcgtcga ggttccgatc gccttcgccg agcgtgcggc gggcgagtcg
     1921 aagatgtcca ccgcgatcaa gctcgagggc gtgggcgtcg tgctgcgcct cgcgcgcgag
     1981 cggtggcgcc cgtcctgatc ccggtccgct acgcgcggcg ggcagccgtc agcgcggcgc
     2041 tcgtcgccgc caccgggacg ctcgcgctct ccgcgaaccg gcacctgagc ggggacgtct
     2101 actggctgct ggccgccgga cgcgagattg cgacgaacgg gctcaccgac acggacccgt
     2161 tcctcacgct caaccacggc cgtgagtggc agaaccagca atggctgacg gagctcgtcc
     2221 tctacggcct cgagcggctc ggcgggcttc ggctcgtctc gctcgcgtac gcgctcgtgc
     2281 tcgggc
//
LOCUS       DASZOB010000466         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_45465, whole genome shotgun sequence.
ACCESSION   DASZOB010000466 DASZOB010000000
VERSION     DASZOB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45465"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..642
                     /locus_tag="VG709_07280"
     CDS             100..642
                     /locus_tag="VG709_07280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWC32914.1"
                     /translation="MGRPPSGGPIPEAVAPAGVEIRPARRRDAAGILEVRRELAAEER
                     FIRTEVVSESVRDTRARLARTWTREAASIVAVADRRVVGHLGVARELQPVTAHVASLG
                     MGVTKALRRRGVGAALLAEAFRWARWAGVEKLALSVYPENEPALRLYERFGFVEEGLL
                     TGHSKKATGYRDELVMARWL"
     gene            658..>1793
                     /locus_tag="VG709_07285"
     CDS             658..>1793
                     /locus_tag="VG709_07285"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWC32915.1"
                     /translation="MAADPREPARVRAGRRATPVPSVPVVTDLASKVLAIKEGDTFLY
                     SDLEGNLDADKELGLGLYHRDTRFLSHFRLEISGRDPVLLSSSAERAYLSHIDLTNPD
                     LYEGEAVAVAQQTLNIRRIRVIDGRLYERIRIKNYNPFGVRVHVGLTFDADFVDVFEI
                     RGVTRDRRGIYGEPLYEGGTLEFTYRGEDDVARITRVSFGIAPDEVEIREDAVEAVFD
                     VNLSPHETKTVPLVVEPIVGETRGDDGADFDAAVHSLRRSYERWESDCTQIRTDNELF
                     NELLTRGLRDLRALTTDVEDSSVIAAGIPWYVAAFGRDTLVTSHQLLMVNPEPARDSQ
                     RVLAAKQGTKVDDWRDEEPGKILHEIRSGELAGAGYIPHTPYYGT"
BASE COUNT          282 a          609 c          649 g          253 t
ORIGIN      
        1 acaggtgctg gcgaacgtcc ttgagctcct cggcgtgtcg gcgccggact cgatggaacg
       61 gctggaggag cagcaagtgc ggctcgaggg gccgaagtag tggggcggcc gccgagcggc
      121 ggcccgatcc ccgaggccgt cgcaccggcc ggcgtcgaga tccgcccggc ccggcggcgc
      181 gacgccgccg ggatcctgga ggtccgcagg gagctcgccg ccgaggagcg gttcatcagg
      241 accgaggtcg tctcggagtc ggtccgagac acccgcgcga ggctcgcccg aacgtggacg
      301 cgcgaggcgg cttcgatcgt cgccgtcgcc gaccggcggg tcgtcgggca cctcggcgtc
      361 gcgcgcgagc tccagcccgt cacggcgcac gtcgcatcgc tcggcatggg cgtcacgaag
      421 gcgttgaggc gccgcggggt cggtgcggcg ctgctcgcgg aggcgttccg gtgggcacgt
      481 tgggccggcg tcgagaagct ggcgctgagc gtctacccag agaacgagcc ggcgctgcgt
      541 ctgtacgagc ggttcgggtt cgtggaggaa ggcctgctca ccggccactc gaagaaggcg
      601 acgggctacc gcgacgagct cgtgatggcg cggtggctct gacttagact ggccgggatg
      661 gctgccgacc cgcgggagcc cgctcgcgtc cgcgccggac ggcgagcgac accggttccg
      721 agcgtccccg tcgtaaccga cctcgccagc aaggtgctgg cgatcaagga aggcgacacg
      781 ttcctgtact cggacctcga ggggaacctc gacgccgaca aggagctcgg gctcggcctg
      841 taccaccgcg acacgcggtt cctctcgcat ttccggctcg agatctccgg acgcgacccc
      901 gtgctgctct cgtcgtccgc ggagcgcgcg tacctctcgc acatcgacct gacgaacccg
      961 gacctctacg agggcgaggc cgtcgcggtc gcgcagcaga cgctgaacat ccggcgcatc
     1021 cgcgtgatcg acggccggct ctacgagcgg atccgcatca agaactacaa cccgttcggg
     1081 gttcgggtgc acgtcgggct cacgttcgac gccgacttcg tcgacgtgtt cgagatccgc
     1141 ggcgtcacgc gggaccggcg agggatctac ggcgaaccgc tgtatgaagg cggcacgctc
     1201 gagttcacct accgcggcga ggacgacgtc gcgcggatca cgcgcgtctc attcgggatc
     1261 gcgcccgacg aggtggagat ccgcgaggac gcggtcgagg ccgtcttcga cgtcaacctc
     1321 tccccccacg agaccaagac cgtgccgctc gtcgtcgagc cgatcgtggg cgagacgaga
     1381 ggcgacgacg gcgccgactt cgatgccgcg gttcacagcc tccgtcgctc gtacgagcgg
     1441 tgggagtcgg actgcacgca gatccgaacc gacaacgagc tgttcaacga gcttctcacg
     1501 cgcgggctgc gggacctccg ggccctcacg accgacgtcg aggattcgag cgtcatcgcg
     1561 gccggcatcc cgtggtacgt ggcagcgttc gggcgagaca cgctcgtgac ctcgcatcag
     1621 ctcctgatgg tcaacccgga gccggcgcgc gactcgcagc gcgtgctggc cgcgaagcag
     1681 gggacgaagg tcgatgactg gcgcgacgag gagcccggca agatcctcca cgagatccgg
     1741 agcggcgagc tggcgggtgc cggctacatc ccgcacacgc cttattacgg gac
//
LOCUS       DASZOB010000467         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_120677, whole genome shotgun sequence.
ACCESSION   DASZOB010000467 DASZOB010000000
VERSION     DASZOB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120677"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VG709_07290"
     CDS             <1..471
                     /locus_tag="VG709_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWC32916.1"
                     /translation="AGARVVGGDPRADRRALAVELGCEDAFDPTAEDPFWKLFAHDEL
                     GARVVMECSGVASALQLALNVARHGGVVGILGIPFEPAPILPAVAAVKELRTFSISGP
                     EKRHIAHALDLLRRRPETGRVVTSVVGLGEADDAMRRLADGEGHDVKVLVDPAR"
     gene            complement(476..1468)
                     /gene="speB"
                     /locus_tag="VG709_07295"
     CDS             complement(476..1468)
                     /gene="speB"
                     /locus_tag="VG709_07295"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWC32917.1"
                     /translation="MKVNRPDEPSFEGIGTFTKLPLVLDLEELAGADVAIVGAPFDET
                     TTGRSGARFGPRAIRLSDMGGGGASPHLDAGVDPFDALNVVDHGDVVVHPGDPARSHE
                     AIRRSVRDVMSAGAVPVVLGGDHSILHPNASAAAEHVAPSSLGIVHFDAHADDAQELY
                     GVRRSHGTPVRLLVEERSVRGEHIVQVGLRGYWPGPEEFAWAREHGLVWFTIGDIEER
                     GWGDVVGDVLSLAARADHVWLSVDIDVVDPGFAPGTGVPEPGGLTPRELLGAVRRIAT
                     EVGFAGMEIVEVSPPYDHADVTALLAHRVVLEALTGLALHRLGRQPRRERPAAR"
     gene            complement(1509..1865)
                     /locus_tag="VG709_07300"
     CDS             complement(1509..1865)
                     /locus_tag="VG709_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWC32918.1"
                     /translation="MARQPFRSVAPGEGALKAVRMMTDRVLVRRPDEDSERRSKGGLV
                     IPATAATVSKRGMWAEVVAVGPNVRNVEPGDEVLLLPDSGIEVEIRGDDYLLVREREL
                     HAVASDHGDPRTGLYL"
     gene            1916..2809
                     /locus_tag="VG709_07305"
     CDS             1916..2809
                     /locus_tag="VG709_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HWC32919.1"
                     /translation="MAEPETVRDLVAEGEARLRRSPAIDHWPSQRERWESDQLMAHAL
                     GIDDDAADPDLAVGTAERRRFRDLIRRRAGGEPMAHIVGWTRFRGIRLEVRPGAFVPR
                     QSSEWLAEQAVRRLRRRSEPVAVDLATGIGPVALAVAWNVRRARVHGTDLSADAIRQA
                     RRNARLLRLSNVDFHRGDMFASLPRAVHGDVDVITIHPPYVPKNEIEDLPLEVRGFEP
                     VDTLTDHSEHGLGLAERAAVDGREWLKRGGWLLVEVSPDRARGIRSLLSRTGYRDVRS
                     TDGWPNVPRVVVGRSERRRSP"
     gene            2823..>3219
                     /locus_tag="VG709_07310"
     CDS             2823..>3219
                     /locus_tag="VG709_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32920.1"
                     /translation="MRRYEVLFVALSVVLVAPNGAAASCAPVTPAAAFKRADLVFLGS
                     ASDSVETGFTDFDVETYFKGVGPERVHVLTGQPQENVAGSGDVHPGTGSRWVVYAFTD
                     PEWGLRIDSCASHAIRGAEVPPARGIGAPA"
BASE COUNT          436 a         1128 c         1206 g          449 t
ORIGIN      
        1 gccggagccc gcgtcgtcgg cggcgatccg cgcgccgacc gcagggcgct cgcggtcgag
       61 ctcgggtgcg aggacgcgtt cgatccgacg gcggaggacc cgttctggaa gctcttcgcg
      121 cacgacgagc tcggggcgcg cgtcgtgatg gagtgctccg gcgtcgcgtc ggcgctccag
      181 ctcgcgctga acgtcgcgcg gcacggaggg gtcgtcggca tcctcgggat cccgttcgag
      241 ccggcgccga tccttcccgc ggtcgctgcg gtgaaggagc tgcggacgtt ctcgatctcc
      301 ggcccggaga agcggcacat cgcgcacgcg ctcgaccttc ttcggcggcg ccccgagacg
      361 ggccgggtgg tgacgtcggt cgtcgggctc ggcgaggccg acgacgcgat gcgccggctc
      421 gcggacggcg aaggccacga cgtaaaggtg cttgtcgacc cggcgcgttg agccgctacc
      481 tcgccgccgg gcgctcccgg cgcggctggc ggccgaggcg atggagcgcg agacccgtca
      541 gcgcctcgag gacgacccgg tgcgcgagca gcgccgttac gtcggcgtgg tcgtacggcg
      601 gtgagacctc gacgatttcc atgccggcga acccaacctc ggtcgcgatc cgacgcaccg
      661 cgccgagcag ctcgcgcggc gtcagtccgc cgggctcggg gacgccggtg cccggcgcga
      721 agccggggtc cacgacgtcg atgtcgaccg agagccagac gtggtcggcg cgcgccgcga
      781 gcgacaggac gtcgcccacg acgtcgcccc accctcgttc ctcgatgtcg ccgatcgtga
      841 accacacgag cccgtgctcg cgcgcccacg cgaactcctc cgggcccggc cagtacccgc
      901 gcagcccgac ctggacgatg tgctcgccgc gcaccgaccg ctcctcgacg aggagccgga
      961 cgggcgtgcc gtgggacctg cggacgccgt agagctcctg cgcgtcgtcg gcgtgggcat
     1021 cgaagtgcac gatcccgagc gagctgggcg cgacgtgctc cgcggcggcc gacgcgttcg
     1081 gatggaggat cgagtggtcg ccgccgagca cgacggggac ggctcccgca gacatcacgt
     1141 ccctgacgct gcgccggatc gcctcgtggc tccgcgcggg atcgccggga tgcacgacga
     1201 cgtcgccgtg gtcgacgacg ttcagcgcat cgaacgggtc gacgccggca tcgaggtgcg
     1261 gcgaagcgcc gccaccgccc atgtccgaca gccggatcgc ccgaggcccg aatcgggcac
     1321 cggagcgacc ggtcgtcgtc tcgtcgaaag gcgccccgac gatcgcgacg tcggcgcctg
     1381 cgagctcctc gaggtcgagg acgagcggca gcttcgtgaa cgtgccgatg ccctcgaacg
     1441 acggctcgtc gggacggttg accttcacgg cgcgccagta tgcgacggcg cggtcgggac
     1501 cgtgagggct agaggtagag gccggtcctc gggtcgccgt ggtcggacgc gaccgcgtgg
     1561 agctcgcgct cgcgaacgag gaggtagtcg tcgccgcgga tctcgacctc gatgccgctg
     1621 tccggaagca gcaggacctc gtcgccgggc tcgacgttgc gaacgttcgg cccgacggcg
     1681 actacctcgg cccacatccc gcgcttgctg acggtcgcgg ccgtcgccgg gatcacaagg
     1741 ccgcccttcg agcggcgctc ggagtcctcg tcggggcgcc gcaccaggac gcggtcggtc
     1801 atcatccgca ccgccttgag cgccccctcg cccggtgcga cggatcggaa cggctggcgt
     1861 gccatcgcgt gaaggatacg cggtcgtcgc gacggctccg ggtaaggttc ccgccgtggc
     1921 ggagcccgag accgttcgcg acctcgtcgc cgaaggcgag gcgcgcttgc gccgctcgcc
     1981 ggcgatcgat cactggccgt cgcagcgcga gcgttgggag tcggaccagc tgatggcgca
     2041 cgcgctcggc atcgacgacg acgccgcgga cccggatctc gccgtcggga cggcggagcg
     2101 gcggcggttc cgcgacctca tccggcgccg tgccggcggc gagccgatgg cgcacatcgt
     2161 cggatggacg aggttcaggg ggatccgtct cgaggttcgc ccgggggcgt tcgtgccgcg
     2221 ccagtcgagc gagtggctgg ccgagcaggc tgtccggcgg ctgcggcggc gctccgagcc
     2281 cgtcgcggtg gacctcgcca ccgggatcgg accggtcgcg ctcgcggtcg cgtggaacgt
     2341 gcggcgggcc cgtgttcacg ggacggatct gtcggccgac gcgatccgtc aggcgcgccg
     2401 gaacgcgagg ctgctgcggc tctccaacgt cgacttccac cggggagaca tgttcgcctc
     2461 gcttcctcga gcggtccacg gagacgtgga cgtcatcacg atccacccgc cgtacgtccc
     2521 gaagaacgag atcgaggacc tcccgctcga ggtccgaggg ttcgaaccgg tcgacacgct
     2581 gaccgatcac tcggagcacg ggctcgggct cgccgaacgc gccgccgtcg acggccgtga
     2641 gtggctgaag cggggcgggt ggctcctcgt cgaggtgagc cccgaccggg cacgcgggat
     2701 caggtcgctg ttgtcgcgga ccggctaccg cgacgttcgc agcaccgacg gctggccgaa
     2761 cgtcccccgc gtcgtcgtcg ggaggtcgga acgacggcgg tcgccgtagc gtcgaaccag
     2821 cggtgaggcg atacgaggtc ctcttcgtcg cgctgagcgt ggtcctggtc gcgccgaacg
     2881 gtgctgccgc ctcgtgcgcg ccggtgacgc cggcggccgc cttcaagagg gccgatctcg
     2941 tcttcctcgg cagtgcctcg gacagtgtcg agaccggttt cacggacttc gacgtcgaga
     3001 cgtacttcaa gggcgtcggg ccggagcggg tgcacgtgct caccggccag ccgcaggaga
     3061 acgtcgccgg ctcaggagac gtgcacccgg ggaccggcag caggtgggtc gtttatgcct
     3121 tcacggatcc agagtggggt ctgaggatcg attcttgcgc ctcgcacgcg atccgaggag
     3181 cagaggttcc gccggctcgc ggcatcggcg cgcccgcgg
//
LOCUS       DASZOB010000468         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_136620, whole genome shotgun sequence.
ACCESSION   DASZOB010000468 DASZOB010000000
VERSION     DASZOB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136620"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VG709_07315"
     CDS             complement(<1..754)
                     /locus_tag="VG709_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWC32921.1"
                     /translation="MDYVRIERPEELRRTAVVCAFRGWNHGGEAATMAASYLRERWGA
                     RRIGDIDPEEFFDFQVTRPVVRLEGGVSRRIDWPANEFFHASLPSRDVILFTGIEPNV
                     RWRTFTGDILDAARTLDAEVVVTLGAFLADVPHSLATPVAGSAPDPADAEALGLSASR
                     YEGPTGIVGVLHDTANRTGMASVSLWAAVPHYLPTGPNPKAALALVEKASALLGVDIE
                     IDTLARAGTAWENQVSATIEENEELKAYVSRLE"
     gene            831..1220
                     /locus_tag="VG709_07320"
     CDS             831..1220
                     /locus_tag="VG709_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32922.1"
                     /translation="MDDEPRRPAQRPPDVTSSALILIVLGIFGLFSNAVVLVNADRVR
                     EASPALVSAAAAAAIAVSGAEVAAGVMVLRMSNAWRVVGIGIAALGAAFLLVTSLRAG
                     PAIGPLLVTAAYVYVVVALVRTRGAFV"
     gene            complement(1224..>2033)
                     /locus_tag="VG709_07325"
     CDS             complement(1224..>2033)
                     /locus_tag="VG709_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWC32923.1"
                     /translation="FASTSNVEAARAVGLRATGTMAHSYVEAFPSELEAFRAFSEDFA
                     DRVVLLVDTYDTERGVEKAIDVARALRDHGVELRGIRLDSGDLGALAKEARATLDAAG
                     FDAGQIFASGALDEHVIAALEREGAPIDAYGVGTKVGVSADAPYLDSVYKLVEYAGRR
                     VAKLSPEKATLPGPKQVWRGRAFDEDVITLRDEDGPEGARPLLERVVTDGRRVGDTDL
                     GAARGRFEEDLGALPKELRTLEPGPYPIRRSDELLRRSDEVRDEIRRRELD"
BASE COUNT          253 a          750 c          718 g          312 t
ORIGIN      
        1 cttccagcct gctcacgtag gccttcagct cctcattctc ctcgatcgtc gccgagacct
       61 ggttctccca cgccgtcccg gcgcgcgcga gcgtatcgat ctcgatgtcg acgccgagca
      121 gcgccgacgc cttctcgacg agcgcgagcg cggccttcgg gttcgggccc gtcgggaggt
      181 agtgcgggac cgccgcccac agcgacaccg aggccatccc cgtccggttc gcggtgtcgt
      241 gcaggacacc gacgatgccg gtcggaccct cgtaccgcga cgcggagagt ccgagggcct
      301 ccgcgtcggc cgggtcgggc gccgagccgg ccaccggcgt cgcgagcgaa tgggggacgt
      361 cggcgaggaa ggcgcccagc gtcacgacga cctccgcgtc gagcgtcctc gcggcgtcga
      421 ggatgtcgcc ggtgaacgtg cgccaccgga cgttgggctc gatgccggtg aacaggatca
      481 cgtcgcgcga cgggagcgac gcgtggaaga actcgttcgc gggccagtcg atccggcggc
      541 tgacgccgcc ctcgaggcgc acgaccggcc gcgtgacctg gaagtcgaag aactcctcgg
      601 ggtcgatgtc gccgatgcgg cgggcgcccc accgctcgcg gaggtaggac gccgccatcg
      661 tcgcggcctc acctccgtgg ttccacccgc ggaacgcgca gacgaccgcc gtgcggcgca
      721 gctcctccgg tcgctcgatc cgtacgtagt ccacggagac agtctctacc gccctcggcg
      781 cgctgtcatt cccgcttgcc tcggcccggt ctcgctggaa cgatcgcgtc gtggacgacg
      841 agccgcggcg gccggcgcag cgccctcccg acgtcacgtc gtcggccctc atcctgatcg
      901 tgctcgggat cttcggcctg ttctcgaacg ccgtcgtgct cgtgaacgcg gaccgggtca
      961 gggaggcctc ccccgctctc gtatccgcgg cggccgccgc cgcgatcgcg gtgtcgggcg
     1021 ccgaggttgc cgcgggcgtg atggtcctgc ggatgtcgaa cgcgtggcgt gtcgtcggaa
     1081 tcgggatcgc ggcgctcggc gccgcgttcc tcctcgtcac ctcgttgcgc gcggggccgg
     1141 cgatcggccc gctcctcgtg accgcggcgt acgtgtacgt cgtcgtggcg ctcgttcgga
     1201 cgcgcggcgc gttcgtgtga cgctcagtcg agctcgcgcc gccggatctc gtcgcggact
     1261 tcgtccgacc gccgaaggag ctcgtccgag cgacggatgg gatacggccc cggctcgaga
     1321 gtccggagct ccttcggcag cgcgccgagg tcctcttcga acctaccgcg ggccgcaccg
     1381 aggtccgtgt cgccgacgcg acgaccgtcc gtgacgacgc gctcgaggag cggccgcgcg
     1441 ccctcgggcc cgtcctcgtc gcgcagcgtg atcacgtcct catcgaaggc tctgccccgc
     1501 cagacctgct tcggcccggg gagcgtggcc ttctcgggag agagcttcgc cacgcgccgg
     1561 ccggcgtact cgacgagctt gtagacgctg tcgaggtacg gcgcgtcggc cgagacgccg
     1621 accttcgttc cgacgccgta cgcgtcgatc ggcgcgccct cccgctcgag tgccgctatg
     1681 acgtgctcgt cgagcgcccc gctcgcgaag atctggccgg cgtcgaaacc cgcggcgtcg
     1741 agggtcgcgc gggcctcctt cgcgagcgcg ccgagatcgc cgctgtcgag tcggatcccg
     1801 cgaagctcga ccccgtgatc ccggagcgcg cgcgcgacgt cgatcgcctt ctcgacgccg
     1861 cgctccgtgt cgtaggtgtc gacgaggagc accacgcggt cggcgaagtc ctcggagaac
     1921 gcgcggaacg cctcgagctc cgaggggaac gcctcgacgt acgagtgcgc catcgttccc
     1981 gtcgcgcgga gcccgacggc gcgcgctgcc tcgacgttgc tcgtcgaggc gaa
//
LOCUS       DASZOB010000469         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_187632, whole genome shotgun sequence.
ACCESSION   DASZOB010000469 DASZOB010000000
VERSION     DASZOB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187632"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VG709_07330"
     CDS             complement(<1..253)
                     /locus_tag="VG709_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32924.1"
                     /translation="MRLGELVAELDAYFRVPDVANDDWSDTFEHLYADPYWRRFAERD
                     YEGRWNGLMVRGGDEIDRAVTCVFPSDRIVSELAPRTLLF"
     gene            complement(250..>1508)
                     /gene="ligD"
                     /locus_tag="VG709_07335"
     CDS             complement(250..>1508)
                     /gene="ligD"
                     /locus_tag="VG709_07335"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWC32925.1"
                     /translation="TRGKGRRERSFGALVLAVRRGDDLVWVGNCGTGVGEGEIERLLG
                     RLRPLERSNPPFRVPPKMPRVRKGDVVCVEPRLVCEVSFVEWTHDEHLRAPSYEGLRD
                     DKLAEDVRRERPIGSEIRRGRRVVRLSNLDKVFWPAEGITKGDLLSYYRAVAPVLLPH
                     VRDRPFTMKRYPEGIEGAHFFQKNAPGHMPDWIPTQSYLSTSRETRRKRMIAYPLVND
                     ELALLWMVNMGCIDLNVWYSRVDKPDRPDFVLFDLDPSPDVGFRETVEVALLVKDTLD
                     ALGLASFPKTSGAEGMHVLVPVERRPTYAATREFAEIVAGALARTHRGLVTTEWSKAK
                     RHGVLVDANQNGEGKTIASVYSVRPKPGAPVSTRLRWDEVDDDLEPGRFTMDVVLARI
                     EREGDVYAPVLGTRQRLDRALAALTG"
BASE COUNT          221 a          547 c          516 g          224 t
ORIGIN      
        1 agaagaggag cgtgcgcggc gccagctcgg agacgatgcg gtcgctcgga aagacgcacg
       61 tgacggcgcg gtcgatctcg tcgccgcctc gcaccatgag cccgttccac cgcccctcgt
      121 agtcgcgctc ggcgaaccgg cgccagtacg ggtcggcgta gaggtgctcg aacgtatccg
      181 accagtcgtc gttcgcgacg tcggggacgc ggaagtacgc gtcgagctcg gcgacgagct
      241 cgcccagcct cacccggtca gcgcggcgag cgcgcggtcg agccgctgcc gcgtgccgag
      301 gacgggcgca tacacgtcgc cctcccgctc gatccgcgcg agcacgacgt ccatcgtgaa
      361 ccgacccggc tcgaggtcgt cgtcgacctc gtcccaccga agccgcgtcg agaccggcgc
      421 gcccggcttc ggccgcaccg agtacacgga cgcgatcgtc ttgccctccc cgttctggtt
      481 cgcgtcgacg agcaccccgt ggcgctttgc cttcgaccac tcggtggtca cgagcccgcg
      541 atgcgtccgc gcgagcgcgc ccgccacgat ctcggcgaac tcacgcgtgg ccgcgtacgt
      601 gggccgccgc tccaccggga cgagcacgtg catcccctcg gcgccgctcg tcttcgggaa
      661 gctcgcgagg ccgagcgcgt cgagcgtgtc cttgacgagg agagcgacct cgaccgtctc
      721 ccggaagccg acgtccggcg acgggtcgag atcgaagagg acgaagtcgg ggcggtccgg
      781 cttgtcgacc cgtgagtacc agacgttcag gtcgatgcag cccatgttca ccatccacag
      841 gagcgccagc tcgtcgttga cgagcgggta ggcgatcatc cgcttgcgcc gggtctcgcg
      901 cgacgtcgac aggtaggact gcgtcgggat ccagtccggc atgtggcccg gcgcgttctt
      961 ctggaagaag tgggcgccct cgatgccctc cgggtaccgc ttcatcgtga acgggcggtc
     1021 cctcacgtgg gggaggagga ccggggcgac ggcgcggtag taggagagca ggtcgccctt
     1081 cgtgatcccc tccgccggcc agaagacctt gtcgaggttc gagagacgca cgacccggcg
     1141 gccgcgccgg atctcgctcc cgatcggccg ctcgcgccgg acgtcctcag cgagcttgtc
     1201 gtcccgcagg ccttcgtacg acggcgcacg caggtgctcg tcgtgcgtcc actcgacgaa
     1261 gctgacctcg cagacgaggc gcggctccac gcagacgaca tcgcccttgc gcacgcgagg
     1321 catcttgggc ggtacgcgga agggcgggtt gctgcgctcg agcggtcgca accgtccgag
     1381 cagccgctcg atctcgccct ccccgacccc ggttccgcag ttcccgaccc agacgaggtc
     1441 atcgccacgc cggaccgcga ggacgagcgc gccgaagctt cgctcgcgcc gccccttgcc
     1501 tctcgtgt
//
LOCUS       DASZOB010000470         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_176929, whole genome shotgun sequence.
ACCESSION   DASZOB010000470 DASZOB010000000
VERSION     DASZOB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176929"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VG709_07340"
     CDS             <1..980
                     /locus_tag="VG709_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC32926.1"
                     /translation="TEWLMREAHAGPAWPAIVAYTTIATIAIVWLTTLAYGAFRISRR
                     HGRGRLAGRRVLITGSAGGIGRAATAALRREGAAVVGLDLVASDGTLATDVRDAEAVR
                     RAVEEAARRMGGIDTVVNNAGIGVAHDAGATPDGVARAVVDVNLFGAWTVTAAAMPYL
                     VRGRGHVVHVSSGLSIATVPWSAAYAASKRALDAYADVLRLEYRGRVSVTTVLPGYIK
                     TGIHDAAAADGVTLDGAVWEDRVGQAGDAILRACADRPRRVSTSIRTAVGLAVARHFP
                     RVADVFVTLGVRRARRGRPAPRLSRPYEPDDPEERSPSAAGIGAGARPS"
     gene            1076..3217
                     /locus_tag="VG709_07345"
     CDS             1076..3217
                     /locus_tag="VG709_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWC32927.1"
                     /translation="MNVARRIVPLVAAAAAVLIVASAGASAAPKGGGRPPTDYLEVAG
                     LSQPDYPKTVQQELHVPVGDGIELYVEVTMPDAAEYPDAGPWPVIMEASPYHGTIADR
                     EGIRIFPDPVDENGNPIGLTGYFAPRGYAVAIVDLRGTGRSSGCLDHLGPNDARDLKA
                     IVEWAASQPWSNGRVGMVGHSYVGSTPSLAAAQNPRGLVTIVPSAGLASMYDHQFQAG
                     VPYFLQWAGPQWSYASLSTLRHLPPQASPATNLALGASGDDFGNHVTETGCGWRTSAL
                     TSGDGQVTGQYKQWHADRDWRAEAAAARMPVFMVHGVNDNAARIPAAEWFFADRSNKR
                     DKVWLGQWDHGSGAHVNRRYTQWQMALHAWLDKHLQQRDVDTGPPVEVFLNDGRVYTS
                     SRWAPLAGDVNLYPDARDGSLSFSPPSGDGQASFLAAANWTRDNRVRFVSDPVTRERL
                     ILGLPELDLDASTTTSQISHLVTTLYREDQFGFRHPMTYCTIQPMLRNGVDTVTPVVP
                     GERMELEPQCFTMAHLVEPGDRLVLEVGTRRPHHVPTPFAVDGRVTVYTGDDGTAFTG
                     SSTQQPRLHRDVPLFDDASLREGPAQQTLQRTINVTAPGGGALGFVTPPFAIEGGHRK
                     VEVVAQQRESANIIVLLQRLVNGTWTTFASSNTIGFDPVRFEGVRPPAGDYRLFVSNR
                     AGQPMLPVDATVTFLNRYGEPGPDQELPAQG"
BASE COUNT          457 a         1199 c         1156 g          446 t
ORIGIN      
        1 acaccgagtg gctcatgcgc gaggcgcatg ccggcccggc gtggccggcg atcgtggcgt
       61 acacgaccat cgcgacgatc gcgatcgtgt ggctcaccac gctcgcgtac ggcgcgttcc
      121 ggatctcccg tcgccacggg cgcgggcggc tcgcggggcg gcgcgtgctg atcacgggat
      181 ccgccggcgg catcggccgc gccgcgacgg ccgcactccg gcgagagggg gccgccgtcg
      241 tcggcctcga tctggtcgct tccgacggaa cgctcgcgac cgacgtccgt gacgccgagg
      301 cggtgcggcg agcggtcgag gaggcggccc ggcggatggg cggcatcgac accgtcgtga
      361 acaacgcggg gatcggcgtc gcgcacgacg ccggcgcgac gccggacggc gtcgcccgcg
      421 ccgtggtcga cgtgaacctc ttcggggcgt ggaccgtgac cgccgccgcg atgccgtacc
      481 tcgtccgagg ccgcggccac gtcgtgcacg tctcgtcggg cctctcgatc gcgacggtgc
      541 cgtggtcggc cgcgtacgcg gccagcaagc gggcgctcga cgcgtacgcc gacgttctcc
      601 ggctcgagta ccgcggccgc gtctcggtca cgaccgtcct tcccggctac atcaagaccg
      661 ggatccacga cgcggcggcc gccgacggcg tcacgctcga cggggcggtg tgggaggacc
      721 gggtcgggca ggccggcgac gcgatcctgc gcgcgtgcgc ggaccgcccg cgccgggtct
      781 cgacgagcat ccggacggcg gtcgggctcg cggtcgcccg tcacttcccc cgggttgcgg
      841 acgtcttcgt cacgctcggc gtccgccgcg cacggagggg tcgtccggcg ccccggctca
      901 gccggccgta cgagccggat gatccggagg agcggtctcc ttccgccgcc ggcatcgggg
      961 ccggcgcgcg cccctcctga gccggacaac tcggacaaca ccgccccgct ctgatatggt
     1021 tcatgtcgtc gtcccgcgag cagcgcggga cctcctacgc ccagggagga aatacatgaa
     1081 cgttgcccgc aggatcgttc cgctcgtcgc ggcggcggcc gcagtgttga tcgtcgcttc
     1141 cgctggcgcc tccgcagcac cgaagggcgg cgggcgtccg ccgaccgact atctcgaggt
     1201 ggctgggctc tcccagccgg attacccgaa gaccgttcaa caggagctcc acgttcccgt
     1261 gggcgacggc atcgagctct acgtcgaggt cacgatgccg gacgccgccg agtacccgga
     1321 cgccggcccc tggccggtga tcatggaggc cagcccgtac cacggcacga tcgccgaccg
     1381 tgaggggatc cggatcttcc ccgacccggt cgacgagaac gggaacccga tcgggctcac
     1441 cggctacttc gcgccgcgcg gctatgccgt cgcgatcgtc gacctgcgag gcaccggccg
     1501 ctcgagcggc tgcctcgacc acctcggccc gaacgacgcc cgcgacctga aggcgatcgt
     1561 cgagtgggcc gccagccagc catggtcgaa cggccgcgtc ggcatggtcg gccactcgta
     1621 cgtcggctcg acgccgagcc tcgccgccgc gcagaacccg cgcggcctcg tgacgatcgt
     1681 tccgagcgcc gggctcgcgt cgatgtacga ccaccagttc caggccgggg tcccgtactt
     1741 cctgcagtgg gcgggcccgc agtggtccta cgcgtcgctc tcgacgctcc gacacctgcc
     1801 tccgcaggcg agccccgcga cgaacctcgc gctcggcgcc tcgggcgacg acttcggcaa
     1861 ccacgtgacc gagaccgggt gcggatggag gacatcggcg ctcacgtccg gcgacgggca
     1921 ggtcaccggc cagtacaagc agtggcacgc ggaccgtgac tggcgcgcgg aagccgcggc
     1981 cgcacgaatg cccgtgttca tggtccacgg cgtcaacgac aacgctgccc ggatccccgc
     2041 cgcggagtgg ttcttcgccg accgctcgaa caagcgcgac aaggtctggc tcggccagtg
     2101 ggaccacggc tcgggcgcgc acgtgaaccg tcgctacacg cagtggcaga tggcgctgca
     2161 cgcgtggctc gacaagcacc tgcagcagcg cgacgtagac accggcccgc cggtcgaggt
     2221 gttcctgaac gacggccggg tgtacaccag cagccgttgg gctcccctcg cgggcgacgt
     2281 caacctctat cccgacgcgc gtgacgggtc gctctcgttc tcgccgccga gcggcgacgg
     2341 gcaggcctcg ttcctcgcgg ccgcgaactg gacgcgcgac aaccgcgtgc ggttcgtctc
     2401 agacccggtg acgcgagagc ggctgatcct tgggctcccg gagctcgacc tcgatgcgtc
     2461 cacgacgacg tcacagatct cgcacctcgt gacgacgctg taccgcgagg accagttcgg
     2521 gttccgccac ccgatgacct actgcacgat ccagccgatg ctgcggaacg gcgtcgacac
     2581 ggtgactccg gtcgtgccgg gcgagcggat ggagctcgag ccgcagtgct tcacgatggc
     2641 gcacctcgtc gagccgggtg accggctggt gctcgaggtc ggcacgcggc gcccgcatca
     2701 cgtcccgacg ccgttcgcgg tcgacggccg cgtgacggtg tacacgggcg acgacggcac
     2761 ggcgttcacg gggtcgagca cgcagcagcc gcggctccac cgcgacgtgc cgctgttcga
     2821 cgacgcgtcg ctccgcgagg gtccggcgca acagacgctg cagcggacga tcaacgtcac
     2881 ggcccccggc ggcggcgccc tcgggttcgt cacgccgccc ttcgcgatcg agggcggcca
     2941 ccgcaaggtc gaggtcgtcg cgcagcagcg cgagagcgcg aacatcatcg tgctgctcca
     3001 gcggctcgtg aacgggacgt ggacgacgtt cgcctcttcg aacacgatcg ggttcgaccc
     3061 ggtccggttc gagggcgtgc ggccccccgc gggcgactac cggctgttcg tctcgaaccg
     3121 tgccggccag ccgatgctgc cggtcgacgc gaccgtgacg ttcctcaacc gctacggcga
     3181 gccgggaccg gaccaggagc tgccggcgca gggctaactc ttcgagttca ccgcgaaggg
     3241 gcgggcttcg gccccgcc
//
LOCUS       DASZOB010000471         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_35160, whole genome shotgun sequence.
ACCESSION   DASZOB010000471 DASZOB010000000
VERSION     DASZOB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35160"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VG709_07350"
     CDS             complement(<1..519)
                     /locus_tag="VG709_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC32928.1"
                     /translation="MDFDVVFLGTAGSVPTARRAPSATLVRRGGERILFDCGEGTQRQ
                     LLRSGIGLVELGEVFLTHYHADHYLGLPGMMKTFALRGRELPLTVYGPPGLADLFVAL
                     QRIFGKLTYRYETVELRPGDVLDRGDYRLEVFRTSHGIAANGYALVEAPRPGRFDVAA
                     ATALRVPAGPLFG"
     gene            complement(644..898)
                     /locus_tag="VG709_07355"
     CDS             complement(644..898)
                     /locus_tag="VG709_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWC32929.1"
                     /translation="MFASRLLVVLAAVAVIAAFALSAPRPTSGAAGTTPYVVRAGDTL
                     WAVASAHYDGDPREAIWRIRERNGLESTLLRPGQVLDMPP"
     gene            complement(1005..1556)
                     /locus_tag="VG709_07360"
     CDS             complement(1005..1556)
                     /locus_tag="VG709_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWC32930.1"
                     /translation="MRPSERERGVRGRRARVPRRPLPARARLIALLGDTHLPRGARRL
                     PADCVRILEACDLILHLGDFTTASVLDDLRARAPVAAVHGNVDDASLRRSLPRRLMVE
                     AEGLRIGLVHDPGSRPGRHERLRGWFPGCDVVAYGHTHLPELEENEGVWIVNPGSPTE
                     RRRAAAHTMVVVDGATPELVELR"
     gene            complement(1472..>1807)
                     /locus_tag="VG709_07365"
     CDS             complement(1472..>1807)
                     /locus_tag="VG709_07365"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWC32931.1"
                     /translation="RFERGEVVSATAGRGAAFLEQMVALDDGARRVGEFAFGMNDAIT
                     ESTLNTLFDEKIGGTMHLALGKSYPETGGKNESALHWDLVCDLRSGSEVYADGELVYR
                     DGRFLRELD"
BASE COUNT          273 a          636 c          654 g          244 t
ORIGIN      
        1 gccgaagagc gggccggccg gaacccgcag cgccgtggcg gcggcgacgt cgaaccgtcc
       61 gggccggggt gcctcgacga gggcgtatcc gttcgcggcg attccgtgac tcgtccggaa
      121 gacttcgagg cgatagtcgc cccgatcgag cacgtcgccc ggcctgagct cgaccgtctc
      181 gtagcggtac gtcagcttcc cgaagatccg ctggagcgcg acgaagagat cggcgagacc
      241 gggcggcccg tacaccgtca gcgggagctc acggccgcga agtgcgaacg tcttcatcat
      301 tcccggcagg ccgagatagt ggtcggcgtg gtagtgcgtg aggaagacct cgccgagctc
      361 cacgaggccg atgcccgagc ggaggagctg gcgctgcgtc ccctcgccgc agtcgaagag
      421 gatgcgctct cccccacgcc gaacgagggt cgcgctcggc gcccggcgcg cggtcggaac
      481 cgagcccgcc gtgccgagga agacgacgtc gaagtccacc gggtgagtct acgagcccgc
      541 gccgcgcgcg ttgcggccgc ctcccgcgtc gtcgcggcgt ccgctgcgaa ttcgctggag
      601 cggtacaagg gcatcgcgcg cgggtgcggg acggaagtcg acgctacgga ggcatgtcga
      661 gcacttggcc gggccgcaga agcgtcgatt cgagcccgtt ccgctctctg atacgccaga
      721 tcgcctcccg cgggtcgccg tcgtagtgcg cgctcgcgac cgcccagagc gtgtcgcccg
      781 cccgcacgac gtatggagtg gtgcctgcag cgccgctcgt gggacgcggg gcggagaggg
      841 cgaacgccgc gatgacggcg acggcggcga gcaccacgag aagcctggag gcgaacatgc
      901 gttcgcatgc tagagcgtcc gtcggacggc acgcggtgga tcgtcggaag cgaggcgccc
      961 atggtgggag aagccagggc cggcgagacc ccatgtcgtc gggctcagcg aagctcgacg
     1021 agctcgggcg tggcgccgtc gacgacgacc atcgtgtgcg cggccgcccg ccggcgttcc
     1081 gtcgggctgc cggggttcac gatccatacg ccctcgttct cctcgagctc cgggagatgc
     1141 gtgtgcccgt aggcgacgac gtcgcacccg gggaaccacc cgcgcaggcg ctcgtggcgg
     1201 cccggccgcg accccgggtc gtggacgagc ccgatccgga ggccctccgc ctcgaccatg
     1261 agccgccggg gcaacgaccg gcgaagcgac gcgtcgtcga cgttcccgtg caccgccgcg
     1321 accggcgcgc gcgctcgcag gtcgtccagg acggacgctg tcgtgaagtc gcccaggtgg
     1381 aggatcaagt cgcacgcttc gaggatccgg acgcagtcgg cgggaaggcg gcgcgccccg
     1441 cgcggaagat gcgtatcgcc gaggagcgcg atcagtcgag ctctcgcagg aagcggccgt
     1501 cgcggtacac gagctcgccg tccgcgtaca cctcgctccc gctccgaagg tcgcacacca
     1561 ggtcccagtg gagcgccgac tcgttcttcc cgccggtctc cgggtacgac ttccccagcg
     1621 ccaggtgcat cgtcccgccg atcttctcgt cgaagagcgt gttgagcgtg gactcggtga
     1681 tcgcgtcgtt catgccgaat gcaaactcgc cgacacgcct tgcgccgtcg tccagcgcga
     1741 ccatctgctc gaggaacgcg gcgccgcggc ccgcggtcgc gctcaccacc tcaccgcgct
     1801 cgaagcg
//
LOCUS       DASZOB010000472         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_45560, whole genome shotgun sequence.
ACCESSION   DASZOB010000472 DASZOB010000000
VERSION     DASZOB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45560"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VG709_07370"
     CDS             complement(<1..645)
                     /locus_tag="VG709_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWC32932.1"
                     /translation="MRLVTYDRAGARRLGALVGEDVVDLPDAVGDPGFPTTMEALVEA
                     ERAPLDAAAEALASVDVERHVVREPRLLVPILPGSLRDFLAFEDHVRAGARRRGEDVP
                     EPWFEMPIYYKGNHRSVYGPDDEIPWPPYTEELDFELEVACVLGGRGRDLSERDAEGL
                     IFGYTLMNDWSARDIQRKEMAVRLGPAKGKDFATSLGPCIATADELDPADVRLVA"
     gene            714..>1631
                     /locus_tag="VG709_07375"
     CDS             714..>1631
                     /locus_tag="VG709_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC32933.1"
                     /translation="MADEYRNYVGGQWVSSRSGQTFESRNPANRDELLGSFPKSGAED
                     VDAAVQAAKDAYHDWMLTPVPKRADYLLRVALILEQRKEELSELMTREMGKTLKESRG
                     DVQEGIDFAHYMAGEGRRFFGKTMPSELAGKFSMTGRHPIGVVGLITPWNFPIAIPLW
                     KIAPAVVSGCASVFKPAEDTPLLATLLVQIFEEVGLPPGVLNLVHGYGEEAGEALVQH
                     PDVRGVSFTGSLEVGKHVAEVCGRQMKRVSLELGSKNVLIVMPDAQMDLAVEAAAWGA
                     FATSGQRCTATSRLVVHEDARSEFTERLLE"
BASE COUNT          250 a          551 c          595 g          235 t
ORIGIN      
        1 ggcgactagc cgcacgtcgg cgggatcgag ctcgtcggcg gtggcgatgc agggcccgag
       61 cgacgtcgcg aagtccttcc ctttcgccgg cccgagccgg accgccatct ccttgcgctg
      121 gatgtcgcgc gccgaccagt cgttcatcag cgtgtacccg aagatgaggc cctcggcgtc
      181 gcgctccgag aggtcgcgcc cgcgaccgcc gagcacgcac gcgacctcga gctcgaagtc
      241 gagctcctcg gtgtacggcg gccacgggat ctcgtcgtcg ggcccgtaca ccgatcggtg
      301 gttgcccttg tagtagatcg gcatctcgaa ccacggttcg gggacgtcct cgccgcgccg
      361 ccgtgcgccg gcgcggacat ggtcctcgaa cgcgaggaag tcacggagcg acccggggag
      421 gatcgggacg agcagccgcg gttcccggac gacgtggcgt tcgacgtcga cgctcgcgag
      481 cgcctcggcg gccgcgtcga ggggcgcgcg ttccgcctcg acgagcgcct ccatcgtcgt
      541 ggggaacccg gggtcgccga ccgcgtcggg gagatcgacg acgtcctcgc ccacgagcgc
      601 cccgagccgt cgcgccccgg cgcgatcgta ggtgacgaga cgcacgcggc gatgatagcc
      661 gcgagttgcc gggcttagga ttggctggcg agcgagccaa aaggaggccg aggatggcgg
      721 acgagtaccg aaactacgtc gggggccagt gggtgtcgtc gagatcgggg cagaccttcg
      781 agtcccgcaa ccccgcgaac cgcgacgagc tcctcggcag cttcccgaag agcggcgcgg
      841 aggacgtcga cgccgcggtg caggccgcga aggacgccta ccacgactgg atgctgacgc
      901 cggttcccaa gcgcgccgac tatctcctgc gcgtcgccct gatcctcgag cagcgcaagg
      961 aggagctctc ggagctcatg acgcgcgaga tgggcaagac gctcaaggag agccgcggcg
     1021 acgtccagga agggatcgac ttcgcccact acatggccgg tgagggccgt cggttcttcg
     1081 gcaagacgat gccgtccgag ctcgcaggca agttctcgat gacgggccgc cacccgatcg
     1141 gcgtcgtggg actcatcacg ccgtggaact tcccgatcgc gatcccgctg tggaagatcg
     1201 cgccggcggt cgtctccggg tgcgcgagcg tcttcaagcc cgccgaggac acgccgctgc
     1261 tcgcgacgct gctcgtccag atcttcgagg aggtcgggct gccgcctggc gtcctcaatc
     1321 tcgtccacgg atacggcgag gaggccggcg aggcgctcgt ccagcacccc gacgtccgcg
     1381 gtgtctcgtt caccggctcg ctcgaggtcg gcaagcacgt cgccgaggtg tgcggacggc
     1441 agatgaagcg cgtgtcgctc gagctcggct cgaagaacgt gctgatcgtg atgccggacg
     1501 cgcagatgga cctcgcggtc gaggcggccg cgtggggcgc gttcgcgacg tccgggcagc
     1561 ggtgcacggc gacgtcgcgg ctcgtcgtgc acgaggacgc gcggtcggag ttcaccgagc
     1621 ggctcctcga g
//
LOCUS       DASZOB010000473         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_45673, whole genome shotgun sequence.
ACCESSION   DASZOB010000473 DASZOB010000000
VERSION     DASZOB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45673"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VG709_07380"
     CDS             <1..981
                     /locus_tag="VG709_07380"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWC32934.1"
                     /translation="QCHDAEDPDGNVELGTTPSGHVVDVSRLVTESDLTVYLNCSTTR
                     GFSGGWKSVCVGLSTYRSIRQHHDPDTMSMSLQRNRMHEILDEMGDIVETALGLRRIF
                     KLETVLTNPLQVHRAFGGTVGATRRAVLDLLRANQAPRRAATDDPLDVVVYGVPDWSP
                     YAAYSFTNPLLSLISTGLGYLGGMIEAAGKPGCTVVIATPCPDRWDDDHHPSYRHVWE
                     GVLPETRDPYEARRRFEPGLAADRGYIDRYRRGNAFHPTHAVMALYPLKRLRHAARVI
                     VAGAEDPSVPRHAGFETAPDVEAALDLAREAHGDTMRVGFVPYPPAVNRS"
     gene            complement(982..>2171)
                     /locus_tag="VG709_07385"
     CDS             complement(982..>2171)
                     /locus_tag="VG709_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWC32935.1"
                     /translation="AGLTAAAAVQTLRDEGFDGRLLVVGEEPHVPYERPPLSKEFLRG
                     EQERASLEIHPEGWFEERAVEMLLGVRATAIDPGATRLTLSSGEDVRYDALLIATGGR
                     PRTLVPPSERVVYLRTIEDADRLRWRLGADRHVLVVGAGFIGSEVAASARDLGSDVTL
                     LDVFDVPLQRALGSQVGSVMARVHEERGVTLRMGDAVASIEERDDAVAVRTDSGRVVE
                     GDVAVVGVSIVPNTELAEAAGIRVANGVVVDERCRTSEPRVFAAGDVANHFHPLFGRQ
                     VRVEHFDNALKHGGAAARNMLGVGEAFADPHWFWSDQYDVNLQFAGDAEGADDLVLRG
                     ALEDRDGIGFYLRDGVVVACVALNRGRDVRRAMKLIASRARPDVAALGDEDVDLRSLV
                     SSR"
BASE COUNT          337 a          803 c          729 g          302 t
ORIGIN      
        1 cagtgtcacg acgccgaaga tccggacggc aacgtcgagc tcgggacgac gccgtcgggc
       61 cacgtggtcg acgtgagccg actcgtcacc gagtccgacc tcaccgtgta cctgaactgc
      121 tcgacgacac gggggttttc gggcggatgg aagtcggtgt gcgtcgggct ctcgacctac
      181 cgctcgatcc gccagcacca cgaccccgac acgatgtcga tgtctctgca gcgcaaccgg
      241 atgcacgaga tcctcgacga gatgggcgac atcgtggaga cggcgctcgg cctccgtcgg
      301 atcttcaagc tcgagaccgt tctcacgaac ccgctgcagg tgcaccgggc gttcggcggc
      361 accgtcggtg caacgcggcg ggccgtactc gatctgctcc gcgcgaacca ggcgccgcgg
      421 cgcgcggcga ccgacgatcc gctcgacgtc gtcgtctacg gcgtccccga ctggagcccg
      481 tacgccgcgt actcgttcac gaacccactg ctgtcgctga tctcgaccgg tctcggctac
      541 ctgggtggca tgatcgaggc ggcggggaag ccaggatgca ccgtcgtgat cgcgacgccg
      601 tgtccggatc ggtgggacga cgaccaccac ccgtcgtacc ggcacgtctg ggaaggcgtc
      661 ctaccggaga cgcgcgatcc ctacgaggcg cggcggcggt tcgagccggg gctcgccgcg
      721 gaccgcggct acatcgaccg ctaccggcgc ggcaacgcct tccacccgac gcacgcggtt
      781 atggcgctct acccgctgaa gcgactgcgt cacgccgccc gcgtgatcgt cgccggcgcc
      841 gaggatccct cggttccccg gcacgccggc ttcgagacgg cgccggacgt cgaggccgcc
      901 ctcgacctcg cgcgcgaggc acacggcgac accatgcgcg tcggcttcgt cccgtaccct
      961 ccagcggtca atcgatcgta gctaccggct cgagacgagc gagcggagat cgacgtcctc
     1021 gtcgccgagc gccgcgacat cgggacgcgc tcgcgacgcg atcagcttca tcgcgcggcg
     1081 gacgtcgcga cctcggttca gcgcgacgca tgcgacgacg acgccgtccc gcaggtagaa
     1141 cccgatgccg tcgcggtcct ccagcgcacc gcggaggacg aggtcgtcgg cgccctccgc
     1201 gtcgccggcg aactgaaggt tgacgtcgta ctgatccgac cagaaccagt gcggatccgc
     1261 gaacgcttcg ccgacaccca gcatgttccg cgcggcggcg cccccgtgct tgagcgcgtt
     1321 gtcgaagtgc tcgacgcgga cctggcgtcc gaagagcgga tggaagtggt ttgcgacgtc
     1381 gccggccgcg aacacgcggg gctcggacgt acggcaccgc tcgtcgacga cgaccccgtt
     1441 cgcgacacgg atgccggcgg cctcggcgag ctcggtgttc gggacgatcg acacgccgac
     1501 gacggcgacg tccccctcca cgacacgacc cgagtccgtc cgtaccgcga cggcgtcgtc
     1561 gcgctcttcg atcgacgcga cggcatctcc catccgaagc gtcacgccgc gctcctcatg
     1621 cacacgcgcc atgaccgacc cgacctggct cccgagtgcg cgctggagcg gcacgtcgaa
     1681 gacgtccagc agcgtgacgt cactcccgag gtcgcgcgcg gaagcggcga cctccgagcc
     1741 gatgaacccg gcgccgacga cgaggacgtg ccggtccgcg ccgagccgcc accggagccg
     1801 gtcggcgtcc tcgatcgtcc gcaggtagac gacgcgctcg gacggcggga cgagcgtgcg
     1861 aggccgccca cccgtcgcga tcagcagcgc gtcgtagcga acgtcctcgc cggaggacaa
     1921 cgtcagccgc gtcgcgccgg ggtcgatcgc cgtcgctcgg acgccgagga gcatctcgac
     1981 cgcgcgctcc tcgaaccatc cctcgggatg gatctcgagc gatgctcttt cctgctctcc
     2041 gcgcaggaac tccttcgaga gcggcggccg ctcgtacggg acgtgcggct cctcgccgac
     2101 gaccaagagc cggccgtcga acccttcgtc ccggagcgtt tgcacggcag ccgccgccgt
     2161 gaggccggca c
//
LOCUS       DASZOB010000474         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_35258, whole genome shotgun sequence.
ACCESSION   DASZOB010000474 DASZOB010000000
VERSION     DASZOB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35258"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VG709_07390"
     CDS             complement(<1..195)
                     /locus_tag="VG709_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWC32936.1"
                     /translation="MTTDRELLGRARRVIPGGVSSPVRAFGAVGGTPVFVERGEGAEI
                     VGVDGRRLVDYVQSWGALLFG"
     gene            complement(206..1183)
                     /gene="hemB"
                     /locus_tag="VG709_07395"
     CDS             complement(206..1183)
                     /gene="hemB"
                     /locus_tag="VG709_07395"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWC32937.1"
                     /translation="MPFPASRPRRLRRSRALRDLVGETDLAARHLVAPMFVKEGIREP
                     APIASMPGHAQHTLESLRKEARETFEAGVRAFVLFGIPERKDAEGSEAWNDDGVAQRG
                     LRALREELGDGAVLIADLCLDEYTDHGHCGVLDQRGDVDNDRTLDLYRRIAVTQTRAG
                     ADLVAPSGMMDGQVAAIRSALDGSGFGGTGIMAYAAKYASAFYGPFRDAAECAPRFGD
                     RSSYQMDPRNADEALREIREDVAEGADIVMVKPALAYLDVIHRAKQEKALPVAAYAVS
                     GEYAMVKAAAERGWIDGEAAMLEILTAIRRAGADLVLTYFAREAAERLG"
     gene            1234..2379
                     /locus_tag="VG709_07400"
     CDS             1234..2379
                     /locus_tag="VG709_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC32938.1"
                     /translation="MWKPARRFVAAAAVVVAAAGAAWLLAGGRGEPVPRLARPVVFVH
                     GMSASAATMGTRGGQFHPLLPRISASFPRPGVCQLDAEPRPWNGSPCVFRYVDDIAAT
                     SEPGARCPCDSQSGILENARKLAADVSEVSANAGGDRVILVGYSMGGAIVRAYLGLFH
                     ERADREVAAAVLVDPATTGSWAFVLDARRHIDDPLFRLLAGAASRFVASRSGVALDSP
                     AFADLQPRSPAYRAVAALPLPRTVSTYTFWGDIRLEIAPAAFGIERTVEIGDVALLPG
                     DPDPTALPALGGQRFRPTGLIPPAEALEIRHTRHLRLTPGDVADLSAACLERTAGCVD
                     TAIELLDIPDSHSNVPTALARIVVEPSALGGRRTLEDAILVAIARNV"
     gene            complement(2376..2756)
                     /locus_tag="VG709_07405"
     CDS             complement(2376..2756)
                     /locus_tag="VG709_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32939.1"
                     /translation="MRKSPLMLVVLLLPACFDAFNPLEDDPEEAGVSVEVVACDVDET
                     SGVATATAEVTSTEPHDSVLLRGELIGEDGTVLHQSATSVQNIEPGRTYRARITFGAL
                     SRQDRRQDLECDVQLDFAADIPGS"
     gene            complement(2777..3772)
                     /gene="trpS"
                     /locus_tag="VG709_07410"
     CDS             complement(2777..3772)
                     /gene="trpS"
                     /locus_tag="VG709_07410"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWC32940.1"
                     /translation="MARVLSGIQPSGEVHLGNYIGAIRHWVRDQDVDDSFILIVDLHA
                     ITVPQDPGTLRRATLDLAATLLAAGIDPGRSTLWVQSQVHEHAELAWVLGCFTGFGEL
                     RRMTQFKDRTAKGGESLASAGLFTYPVLQAADILLYRAERVPVGEDQRQHLELTRDVA
                     QRFNGRFGEAFVVPEAAVPQVGARIMDLQAPTSKMSKSAESPAGTIRVTDTPDEITRK
                     MRTAVTDSGREIVASPEKPAITNLLTIYSVSTGRSVEELERDHEGKGYADLKSGLADA
                     LVEFLRPLRERYRALSADPGEIERLLAVGADKASAVAAKTLDDVYEKLGFLRPAT"
     gene            complement(3765..>4223)
                     /locus_tag="VG709_07415"
     CDS             complement(3765..>4223)
                     /locus_tag="VG709_07415"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWC32941.1"
                     /translation="LRTVGAAPDLVPPTYTTEALGEAFPQGSGEVLMPRADIATNELE
                     DAIRSKGWTPVRVDAYRARAAESIPAEVRPALDGGTVDAVTFTSASTVDGFVRAAGVV
                     RGPAVVCIGPVTADAARRAGFAVDAVAEPHTIEGLVDAVERALRDRRSDG"
BASE COUNT          539 a         1588 c         1493 g          603 t
ORIGIN      
        1 gccgaagagc aaggcgcccc acgactgcac gtagtcgacc aaccggcgtc cgtcgacgcc
       61 gacgatctcg gcgccctccc cgcgctcgac gaacaccggc gtgccgccga ccgcgccgaa
      121 cgctcgcacg ggtgagctca cgccgccggg gatcacgcgg cgcgcgcgac ccaggagctc
      181 tcggtccgtc gtcacgcgcg cccgctcagc cgaggcgctc ggcggcctcg cgcgcgaagt
      241 acgtgaggac gaggtcggcg ccggcgcgcc ggatcgcggt gaggatctcg agcatcgcgg
      301 cttccccgtc gatccagcct cgttctgccg ccgccttcac catcgcgtac tcgcccgaga
      361 cggcgtacgc ggcgaccggg agcgccttct cctgcttcgc gcggtggatg acgtcgaggt
      421 acgcgagcgc gggcttcacc atcacgatgt cggcgccctc ggcgacgtcc tcgcggatct
      481 cccggagcgc ctcgtcggcg ttccgcgggt ccatctggta cgacgaccgg tcgccgaagc
      541 gcggggcgca ctcggccgcg tcgcggaacg ggccgtagaa cgccgaggcg tacttcgcgg
      601 catacgccat gatgccggtc ccgccgaagc cggatccgtc gagcgccgag cggatcgccg
      661 cgacctggcc gtccatcatc cccgacggcg cgacgaggtc ggcgccggcg cgcgtctggg
      721 tcacagcgat gcgtcgatag agatccagcg tccggtcgtt gtcgacgtcc ccgcgctggt
      781 cgagcacgcc gcagtgcccg tggtcggtgt actcgtcgag gcagaggtcg gcgatcagca
      841 cggcgccgtc gccgagctcc tcccgaagcg cgcggagccc gcgctgggcg accccgtcgt
      901 cgttccacgc ttcgctcccc tccgcgtcct tgcgctcggg gatgccgaac agcacgaacg
      961 cgcggacgcc cgcctcgaac gtctcacgcg cctccttccg cagcgactcg agggtgtgct
     1021 gcgcgtgccc cggcatcgac gcgatcggcg cgggctcgcg gataccttcc ttgacgaaca
     1081 tcggggcgac gaggtgacgc gccgcgaggt ccgtctcgcc gacgaggtcg cgcagcgcac
     1141 ggctccgccg gagcctgcgg ggacggctgg ctgggaacgg cacggcccga tggtaagagg
     1201 tcccgtggcc cggcgtcagg cgatgatggg cggatgtgga agcccgcgcg ccggttcgtc
     1261 gccgccgcgg cggtcgtggt ggcggccgcg ggagcggcct ggctgctggc cggcggccgc
     1321 ggcgagccgg tgccgcgcct cgcccgcccg gtcgtcttcg tccacggcat gagcgccagc
     1381 gccgccacga tggggacgcg ggggggccag ttccatccgc tgctgccccg gatctccgcc
     1441 tcgttcccac ggccgggcgt gtgccagctc gatgccgagc cccggccctg gaacggaagc
     1501 ccgtgcgtgt tccgctacgt cgacgacatc gcagcgacga gcgagcccgg cgcccggtgc
     1561 ccctgcgact cccagagcgg catcctcgag aacgcgcgga agctcgcggc cgacgtctcg
     1621 gaggtctcgg cgaacgccgg cggcgatcgc gtgatcctcg tcggctacag catgggcggc
     1681 gcgatcgtcc gcgcgtacct gggccttttc cacgagcgtg ccgaccgcga ggttgccgcc
     1741 gcggtcctcg tcgatcccgc gacgaccggc tcgtgggcgt tcgtgctcga cgctcggcgc
     1801 cacatcgacg atccgctgtt ccgcctcctc gccggggcgg cgtcccgatt cgtggcgtcg
     1861 cggtcgggcg tcgcgctcga cagcccggcg ttcgccgacc tccagccgag gagccccgcg
     1921 taccgcgccg tggccgcgct cccgctcccg cggaccgtga gcacgtacac gttctgggga
     1981 gacatccggc tcgagatcgc tccggcggcc ttcgggatcg agcgcacggt ggagatcggc
     2041 gacgtcgcgc tcctccccgg cgacccggat cccacggcgc ttccggccct cggcggccag
     2101 cggttccgcc cgaccggact catcccgccg gcggaggcgc tcgagatccg ccacacgcgg
     2161 cacctccgcc tcacgccggg cgacgtcgcg gatctctccg ccgcgtgcct cgaacgcacc
     2221 gccggctgcg tcgacaccgc gatcgagctc ctcgacatcc ccgactcgca ctccaacgtg
     2281 ccgaccgcgc tcgcgcggat cgtcgtcgag ccctcggcgc tcgggggccg gcggacgctc
     2341 gaggacgcga tcctcgtggc gatcgcccgg aacgtctagc ttccggggat gtcggcggcg
     2401 aagtcgagct gcacgtcgca ctcgaggtcc tggcgccggt cctgccgcga caacgccccg
     2461 aacgttatcc gcgcgcggta ggtcctgccg ggctcgatgt tctggacgct cgtagcgctc
     2521 tggtggagca cggtgccgtc ctccccgatc agctcgccgc gcaggaggac gctgtcgtgc
     2581 ggctcggtgc tcgtgacctc ggcggtcgcg gtcgcgacgc cgctcgtctc gtccacgtcg
     2641 caggcgacga cctcgacgct gaccccggcc tcctccggat cgtcctcgag cgggttgaac
     2701 gcgtcgaagc aggccggaag cagcaggacg acgagcatga gcggcgactt ccgcatggga
     2761 cctgcctccc tcgcgactag gtggccgggc ggaggaagcc tagcttctcg tagacgtcgt
     2821 cgagcgtctt cgccgcgacg gcgctggcct tgtcggcgcc gaccgcgagg agccgttcga
     2881 tctcacccgg gtcggccgac agcgcccggt agcgttcccg cagcggccgg aggaactcga
     2941 cgagggcgtc ggcgagcccc gacttcaggt ccgcgtaccc cttcccctcg tgatcgcgct
     3001 cgagctcctc gacgctccga ccggtcgaga ccgagtagat cgtgaggagg ttcgtgatcg
     3061 cgggcttctc cggcgaggcg acgatctcgc gcccggagtc cgttacggcg gtgcgcatct
     3121 tgcgcgtgat ctcgtcgggc gtgtcggtca cgcggatcgt gccggccggc gactcagcgg
     3181 acttcgacat cttcgacgtc ggggcctgca ggtccatgat ccgtgccccc acctgcggaa
     3241 cggccgcctc gggcaccacg aaggcctccc cgaaccggcc gttgaaccgc tgggcgacgt
     3301 cgcgtgtgag ctcgaggtgc tggcgctggt cctcgccgac cggcacgcgc tccgcgcggt
     3361 agaggaggat gtcggcggcc tggagcacgg gatacgtgaa caggccggcg ctcgcgagcg
     3421 actccccgcc cttcgcggtc cggtccttga actgggtcat ccggcggagc tccccgaacc
     3481 ccgtgaagca cccgagcacc cacgcgagct cggcgtgctc gtggacctgc gactgcaccc
     3541 atagcgtgct ccggcccggg tcgatcccgg ccgcgaggag cgtcgccgcc aggtcgagcg
     3601 tcgcgcgacg gagcgtcccc ggatcctgcg ggaccgtgat cgcgtggagg tccacgatga
     3661 ggatgaacga gtcgtcgacg tcctggtcgc ggacccagtg gcggatcgcg ccgatgtagt
     3721 tgcccaggtg gacctcgccg ctgggctgga tccccgacag caccctagcc atccgacctc
     3781 ctgtcacgga gcgcccgctc gaccgcgtcg acgagacctt cgatcgtatg cggctccgcg
     3841 accgcgtcga cggcgaaccc cgcccggcgc gccgcatcgg cggtgaccgg gccgatgcag
     3901 acgacggccg gaccccgcac gactccggcc gcgcggacga agccgtcgac ggtcgaggca
     3961 gaggtgaacg tgaccgcgtc caccgtgccg ccgtcgagcg ccgggcggac ctcggccggg
     4021 atcgactcgg ccgccctcgc ccggtaggcg tcgacgcgga cgggcgtcca tcccttcgaa
     4081 cggatcgcgt cctcgagctc gttcgtcgcg atgtcggccc gcggcatcag cacctcaccc
     4141 gagccctgcg ggaaagcctc gccgagcgcc tcggtcgtgt acgtcggcgg cacgaggtcc
     4201 ggcgcggcgc cgacggtcct gag
//
LOCUS       DASZOB010000475         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_137145, whole genome shotgun sequence.
ACCESSION   DASZOB010000475 DASZOB010000000
VERSION     DASZOB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137145"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="ftsX"
                     /locus_tag="VG709_07420"
     CDS             <1..837
                     /gene="ftsX"
                     /locus_tag="VG709_07420"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWC32942.1"
                     /translation="ALRRNALVTFAAVSTAFISLFLLGGSMLVRKQVDVMAGAWGNKV
                     EVAVYLRDDSSRQEIDALNRKISEMPEVNAVYFESKREAFQNFRQQFADSEDLVQNVS
                     PDAIPASYRVKLVNPEEFEVISARLQGDPAVLDVRDEREVLERVFAVTEVLRVGVMAV
                     AIVMLLSAAGLIGNTVRMAVFARRKEIAIMKLVGATNWFIRVPFLIEGMAQGLFGGLL
                     AILALFAMKFLFLDKLREDILFIPWVGTNAIMSTVPILLGAGVVIAAVASLFAMRRFL
                     EV"
     gene            884..2161
                     /locus_tag="VG709_07425"
     CDS             884..2161
                     /locus_tag="VG709_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWC32943.1"
                     /translation="MTSKLSIRRRAAVGLVSTLFLFAAAGPEVAAHPPRHHRERLEEI
                     EREVDRKQNRIEELESEREDVVGLLEESDAEREDLGERIAELGADLSESRDRLATVEA
                     ELDLARQAVVQWGNRLERARQRLREQQRTFQNRAATAYMLGPAGYLNLILGAEDLGSL
                     SDRIVYLDSVLTIDSNMVHGLETTRSLVGNHANLVSSYESEVARHEERVQAQVNRIEQ
                     LRAEQQAARAAVLDEIADQQSLLDEIETTKAKYEEAVADLHAQSSQIQSLLQSTGSSG
                     SGNVPANDDGGGGGDGGGGGGGGQLFWPTTGSIGSGFGWRTHPIFGTQRFHAGVDIGG
                     ACGQPIWAGEDGTVVSAGYSGGYGNLTVIDHGDGLATAYAHQSSFGVSSGQSVSRGQT
                     IGYVGTTGWSTGCHLHFEVRVNGSPVDPVPYLT"
     gene            2172..2639
                     /gene="smpB"
                     /locus_tag="VG709_07430"
     CDS             2172..2639
                     /gene="smpB"
                     /locus_tag="VG709_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWC32944.1"
                     /translation="MTSGDRTVATNRRARHDYAIEESFEAGIVLTGPEVKSLRAGRAS
                     LADAYGRVTGGEVWVDNLHIPPYEQADPRTQEPKRPRKLLLRRDEIARLIGKTQERGL
                     SLIPLKIYFTRGLAKVEMGLGRGKRRFEKRQDIAEREHRREIERGFARRRRGE"
BASE COUNT          437 a          928 c          976 g          410 t
ORIGIN      
        1 gcactgcggc gcaacgcgct cgtcacgttc gccgccgtct cgaccgcctt catctcgctg
       61 ttcctgctcg ggggctccat gctcgtgcgc aagcaggtcg acgtgatggc cggcgcgtgg
      121 gggaacaagg tcgaggtcgc cgtctatctc cgcgacgact cgtcgcggca ggagatcgac
      181 gcgctgaacc gcaagatcag cgagatgccg gaggtcaacg ccgtctactt cgaatcgaag
      241 cgggaggcgt tccagaactt ccgccagcag ttcgccgact ccgaggatct cgtccagaac
      301 gtcagccccg acgcgatccc cgcgtcgtac cgcgtgaagc tcgtgaaccc cgaggagttc
      361 gaggtgatca gcgcgcggct ccagggcgat cccgccgtcc tcgacgtgcg cgacgaacgc
      421 gaggtcctcg agcgcgtctt cgccgtgacc gaggtgctcc gcgtgggcgt catggccgtc
      481 gcgatcgtga tgcttctctc cgccgcgggc ctcatcggga acacggttcg catggcggtc
      541 ttcgcgcgtc gcaaggagat cgcgatcatg aagctcgtcg gtgcgacgaa ctggttcatc
      601 cgcgtgccgt tcctcatcga ggggatggcc caggggctgt tcggagggct gctcgcgatc
      661 ctcgcactgt tcgcgatgaa gttcctcttc ctcgacaagc tccgtgagga catcctgttc
      721 atcccatggg tcgggaccaa cgcgatcatg tccacggtgc cgatcctcct cggcgccgga
      781 gtggtgatcg cggccgtggc gagcctgttc gccatgcgtc ggttcctcga ggtctagggt
      841 cgttgctcgg gccatatgcg ctgcctacga tgacctcgcg ccgatgacgt cgaagctgtc
      901 gatccgccgc cgcgccgccg tggggctcgt ctccacgctg ttcctgttcg ccgccgccgg
      961 gcccgaggtc gcagcgcacc caccccgcca ccaccgcgag cgtctggagg agatcgaacg
     1021 ggaggtcgac cggaagcaga accggatcga ggagctcgag agcgagcgcg aggacgtggt
     1081 cgggctgctc gaggagtcgg atgccgagcg cgaggacctc ggcgagcgga tcgccgagct
     1141 cggtgccgat ctgtcggagt cgcgcgacag gttggccacc gtcgaagcgg agctggatct
     1201 cgcccggcag gccgtcgtcc agtggggcaa ccgcctcgag cgcgcgcgac agcggctgcg
     1261 tgagcagcag cgcacgttcc agaaccgcgc cgccactgcc tacatgctgg ggccggccgg
     1321 gtacctgaac ctgatcctcg gcgccgagga cctcgggtcg ctctccgacc gcatcgtcta
     1381 cctcgacagc gtcctgacga tcgactcgaa catggtccac gggctcgaga cgacgcggtc
     1441 gctggtcggc aaccacgcga acctcgtctc gtcgtacgag tccgaggtcg cgcggcacga
     1501 ggagcgcgtc caggcgcagg tcaaccggat cgagcagctc cgcgcggagc agcaggcggc
     1561 cagggccgcg gtgctcgacg agatcgcgga ccagcagtcg ctgctcgacg agatcgagac
     1621 gaccaaggcg aagtacgagg aggcggtcgc agacctccac gcgcagtcct cgcagatcca
     1681 gtcgctgctc cagtccaccg ggtcgagcgg atcgggcaac gttcccgcga acgacgacgg
     1741 aggtggcggc ggtgatggtg gcggcggcgg aggaggaggg cagctgttct ggccgacgac
     1801 cgggtcgatc ggatccggct tcggctggcg cacgcacccg atcttcggga cgcagcgctt
     1861 ccacgccggc gtcgacatcg gcggcgcgtg cgggcagccg atctgggcgg gcgaggacgg
     1921 gaccgtcgtg agcgccggct acagcggcgg gtacgggaac ctcaccgtga tcgaccacgg
     1981 cgacgggctc gcgacggcgt acgcgcacca gtcgagcttc ggcgtctcga gcgggcaatc
     2041 ggtgagccgc ggccagacga tcgggtacgt cggcacgacc ggttggtcga cgggctgcca
     2101 cctccacttc gaggtgcgcg tgaacggctc gccggtcgac ccggtgccct acctcacgta
     2161 gcgtccgagg cgtgacctcg ggcgatcgca ccgttgcgac gaaccgtcgc gcccgccacg
     2221 actacgcgat cgaggagtcg ttcgaggcgg ggatcgtgct cacgggcccg gaggtgaagt
     2281 cgctccgggc cggccgagcg tcgctcgccg acgcctacgg ccgcgtgacc ggcggcgagg
     2341 tgtgggtgga caacctccac atcccgccgt acgaacaggc cgacccacgg acgcaggagc
     2401 cgaagcgccc gcggaagctg ctgcttcgcc gcgacgagat cgcgcggctc atcggcaaga
     2461 cgcaggagcg cgggctgtcg ctgatcccgc tcaagatcta cttcactcga gggctcgcca
     2521 aggtcgagat ggggctcggg cgcgggaagc ggcggttcga gaagcgccag gacatcgccg
     2581 agcgcgagca ccggcgcgag atcgagcgcg gcttcgcccg ccgccgccgc ggcgaatagg
     2641 attccgcgca tgccgacgtt cgaggaactc gacaagctct cgacagagga gctgcgccag
     2701 cgcgcgttcc ggaccgcacg cgacaggctc gacgtgcgct tcttctggaa c
//
LOCUS       DASZOB010000476         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_15269, whole genome shotgun sequence.
ACCESSION   DASZOB010000476 DASZOB010000000
VERSION     DASZOB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15269"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..470)
                     /locus_tag="VG709_07435"
     CDS             complement(48..470)
                     /locus_tag="VG709_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32945.1"
                     /translation="MSAAGAGEPERPYRRAHFSEVPTFASDLVEAEWKPVRHHFGVSA
                     FGTNAYVARGEGELLIEEHAEGDGGHEELYVVLDGEARFTVDGESFAAPAGTLVFVPP
                     PLGRAAVAAKAGTAVLAVGAIPGAAFEVSRWEAARTVG"
     gene            complement(467..1540)
                     /locus_tag="VG709_07440"
     CDS             complement(467..1540)
                     /locus_tag="VG709_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWC32946.1"
                     /translation="MTYALWGRATSQAFEAFAKELGEALRARGFELHDGDPRDADLVL
                     NLIDPARPRPFRRRSRGTFVAAIHEQPAVPDDILKTNYPLLVHALANIVLCYVPNQGV
                     CFTTMERGHYGVHADDGERGLADGVIERLAPLALSKLVIDNDFRTDLEPELWDGDEVT
                     EEISEAGKRMGDLDLLPAPFPIEELLSERELRHVKRLYGIGGLSYGNLSQRKDERRFW
                     MSASGVDKTRLDVPGRDILLVSDYDAERNRMVLSVPPTVEPRRVSVDAIEHWMIYQAH
                     PDVGAILHVHAWMQDIPATDINYPCGTAELATSVAELIDREPDAAHAVIGLRNHGITV
                     TGESLTEILDRIEPRVLRQVPMS"
     gene            complement(1551..>2065)
                     /gene="acnA"
                     /locus_tag="VG709_07445"
     CDS             complement(1551..>2065)
                     /gene="acnA"
                     /locus_tag="VG709_07445"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935475.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWC32947.1"
                     /translation="IRLRNLLGPAGSDLPEGGVTLKFSDEREPREMSIYEASLAYMEE
                     GTPLVVLAGKEYGSGSSRDWAAKGPRLLGVKAVIAESYERIHRSNLVGMGILPLQFSP
                     GESAESRGLTGRETVAIRGVENGEAREVTVRADETEFAVRVRLDTPRERDYLRAGGIL
                     PYVLDRLLSE"
BASE COUNT          284 a          751 c          718 g          312 t
ORIGIN      
        1 cagtacctgc gctagcgagg ggacgcgttg cgcgatgtgc gcgcccgcta tccgaccgtg
       61 cgcgcggcct cccagcggga gacctcgaac gcggcgcccg ggatcgcgcc gacggccagg
      121 acggccgtgc ccgctttcgc cgcgaccgcg gcgcgcccga gcggcggcgg gacgaagacg
      181 agcgtcccgg ccggcgcggc gaacgactcg ccgtccaccg tgaagcgggc ctcgccgtcg
      241 agcacgacgt agagctcctc gtggccgccg tcgccctcgg cgtgctcctc gatcaggagc
      301 tcgccctcgc cccgcgcgac gtaggcgttc gtcccgaacg cggagacgcc gaagtggtgt
      361 cgcaccggct tccactcggc ctcgacgagg tcgctcgcga acgtgggaac ctcggagaag
      421 tgagcccggc ggtacggccg ctcgggttcg cccgccccgg ccgcgctcac gacatcggga
      481 cctggcggag cacccgcggc tcgatccggt cgaggatctc cgtcaggctc tcgccggtca
      541 ccgtgatgcc gtgatttcga agcccgatca cggcgtgagc cgcgtccggc tcgcggtcga
      601 tgagctcggc gacgctcgtc gcgagctccg cggtcccgca cgggtagttg atgtccgtcg
      661 cgggaatgtc ctgcatccac gcgtggacgt ggaggatcgc gcccacgtcc gggtgggcct
      721 ggtagatcat ccagtgctcg atcgcgtcca cggacacgcg gcgaggctcc acggtcggcg
      781 gcacgctcaa gaccatccgg ttccgctcgg cgtcgtagtc ggagacgagg aggatgtcgc
      841 gcccgggcac gtcgagccgc gtcttgtcga cgccgctcgc actcatccag aagcgccgct
      901 cgtccttccg ctgcgagagg ttcccgtacg acaggccgcc gatcccgtag agccgcttca
      961 cgtggcggag ctcccgctcg ctgaggagct cctcgatcgg gaacggcgcc ggaaggaggt
     1021 cgaggtcgcc catccgcttg cccgcctcgc tgatctcctc cgtcacctcg tcgccgtccc
     1081 agagctccgg ctcgaggtcg gttcggaagt cgttgtcgat cacgagcttc gaaagcgcga
     1141 gcggggctag ccgctcgatc accccgtcgg cgaggccccg ctcgccgtcg tctgcgtgca
     1201 cgccgtagtg gccgcgctcc atggtggtga agcagacgcc ctggttcggc acgtaacaga
     1261 ggacgatgtt cgcgagcgcg tggacgagga gggggtagtt cgtcttcagg atgtcgtcgg
     1321 gcacggccgg ctgctcgtgg atcgcggcga cgaacgtccc gcgcgagcgg cggcggaacg
     1381 gcctcgggcg cgcaggatcg atcaggttga ggacgaggtc ggcgtcgcga gggtcgccgt
     1441 cgtgcagctc gaaaccgcgc gcccggagcg cctcgccgag ctccttggcg aacgcctcga
     1501 acgcctgcga ggtggcgcgt ccccagagcg cgtacgtcat cgcgccaatc ctattcggac
     1561 aggagccggt cgaggacgta agggaggatg ccgccggcgc gtaggtagtc ccgctcgcgc
     1621 ggcgtgtcca gccgcacgcg cacggcgaat tccgtctcgt ccgcgcgcac cgtcacctcg
     1681 cgcgcctcgc cgttctcgac gcctcgaatc gcgaccgtct cccgcccggt cagcccgcgc
     1741 gactcggcgc tctcgcccgg cgagaactgg agcgggagaa tccccatccc gacgaggttc
     1801 gagcggtgga tccgctcgta gctctccgcg atcacggcct tgacgccgag gagccgcggc
     1861 cccttcgccg cccagtcgcg tgacgagccc gagccgtatt ccttgcccgc cagcacgacc
     1921 agcggcgttc cctcctccat gtatgcgagc gaggcctcgt agatcgacat ctcgcgcggc
     1981 tcgcgctcgt cgctgaactt cagcgtcacg ccgccctccg gcagatcgga tcccgccggc
     2041 cccagcaggt tgcgcaggcg gatgt
//
LOCUS       DASZOB010000477         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_137246, whole genome shotgun sequence.
ACCESSION   DASZOB010000477 DASZOB010000000
VERSION     DASZOB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137246"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VG709_07450"
     CDS             <1..106
                     /locus_tag="VG709_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HWC32948.1"
                     /translation="GFTPVLWPDFNRQHLFEAIRDYQKRERRFGAVGE"
     gene            110..1225
                     /locus_tag="VG709_07455"
     CDS             110..1225
                     /locus_tag="VG709_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HWC32949.1"
                     /translation="MEERWLTIGIEEEFQIVDRDGELRAAIDEILAETQSTDGLTEAI
                     KADVMQSVVEIGTNICNDIGEARDELTRLRGALAGILSKHDLRLVSAGTHPFSKWYEQ
                     LVTDKERYKVLEEEMQDLTRELLIFGLHVHVGIPDDELRIEIMNEARYFLPHLLALST
                     SSPFWMGRRTGIKSYRSVLWSRFPRTGITPHFGSFSEYREFVDLLVKTKCIEDGSKIW
                     WDMRAHHLYPTIEFRVCDAATRVEETLCLAALIQSVCGLLLRFRKRNLGWRQYMPRLI
                     RENKWRAMRFGLDGSLIDFGKQEEVPERELALEFLDVLEDVPDELGCRKEVEHVRTIV
                     EEGTSADRQLRVFEETRDLKAVVDHLAAETMDGVPAP"
     gene            1246..1707
                     /locus_tag="VG709_07460"
     CDS             1246..1707
                     /locus_tag="VG709_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWC32950.1"
                     /translation="MARYFAYGSNMDERQMRRNAPAAKVLGHARLDGYRMEFTVYSDR
                     WEGGAANIEPDPDGHVWGIVWDVADDDLDALDTYEGHPTFYRREQVTVETSDGPLECT
                     TYRIAHQRGYVRPTDDYLYRMRSAIRVHGLPPEALDILEAAARPPGPHIST"
     gene            1715..>2585
                     /locus_tag="VG709_07465"
     CDS             1715..>2585
                     /locus_tag="VG709_07465"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWC32951.1"
                     /translation="MPDLSWDQVRAWRVARHGLDERSTRRRLLSVASEICGVHAQVGS
                     AAELSLWARVEGVTPRDVRDALWKTKRLVRSWMMRGTIHLLPARDFPLYVAALATHDR
                     WWKGAWLRAVGMSADELGALLGAIREALSDEPITREQLASRVGHLVGDHAREHLLSGW
                     GSLLKPAAFNGSLCSGPPRGQNVTFVRPDLWLGRWEEHDGDQAVREVFRRYLRAYGQS
                     HHEEFAAWWGGAPAFARRARLAIEEELREIDVDDMKRWVLRRDLAAMQRTAPLRGSVR
                     LLPHFDAYVRGFRP"
BASE COUNT          409 a          867 c          930 g          379 t
ORIGIN      
        1 ggggttcacg cccgtgctat ggcccgactt caaccggcaa catctcttcg aggcgatccg
       61 cgactaccag aagcgagagc gccgcttcgg cgcggtgggg gagtaggcca tggaggaacg
      121 ctggctcacg atcgggatcg aggaggagtt ccagatcgtc gaccgggacg gcgagctgcg
      181 cgcggcgatc gacgagatcc tcgcggagac gcagtcgacg gacggcctga ccgaggcgat
      241 caaagccgat gtcatgcagt cggtggtcga gatcgggacg aacatctgca acgacatcgg
      301 tgaggcgcgg gacgagctca cgcggctccg cggcgcgctc gccggcatcc tctcgaagca
      361 cgacctgcgc ctcgtctcgg cggggacgca cccgttctcg aagtggtacg agcagctcgt
      421 caccgacaag gagcggtaca aggtcctcga ggaggagatg caggacctca cgcgggagct
      481 cctgatcttc gggctgcacg tgcacgtcgg catccccgac gacgagctgc ggatcgagat
      541 catgaacgag gcgcgctact tcctcccgca tctcctcgcg ctgtcgacgt cgagcccgtt
      601 ctggatgggg cgccgcaccg gcatcaagtc gtacaggagc gtgctgtggt cgcggttccc
      661 ccggacgggg atcacgccgc acttcggctc gttcagcgag taccgggagt tcgtcgacct
      721 gctcgtcaag accaagtgca tcgaggacgg ctcgaagatc tggtgggaca tgcgggcgca
      781 ccacctgtac ccgacgatcg agttccgcgt ctgcgacgcc gccacacgcg tggaggagac
      841 gctgtgcctc gcggcgctca tccagtcggt gtgcggcctc cttctccggt tccgcaagcg
      901 caacctcggg tggcggcagt acatgccacg gctgatccgc gagaacaagt ggcgcgcgat
      961 gcggttcggg ctcgacggga gcctcatcga tttcggcaag caggaggagg tgccggagcg
     1021 ggagctcgcg ctcgagttcc tcgacgtgct cgaggacgtg cccgacgagc tcggatgccg
     1081 caaggaggtc gagcacgtcc gcacgatcgt cgaggagggg acgagcgccg accggcagct
     1141 gcgggtcttc gaggagaccc gggacctcaa ggccgtggtg gaccacctcg ccgccgagac
     1201 gatggacggc gtcccggccc cgtagcgcca ctagaatcgc ggcagatggc gcggtacttc
     1261 gcctacggca gcaacatgga tgagcgacag atgcgccgga acgcgcccgc ggcgaaggtg
     1321 ctcggacacg cgcggctcga cggctaccgg atggagttca cggtgtattc cgaccggtgg
     1381 gagggaggcg ccgcgaacat cgagccggat cccgacggcc acgtctgggg gatcgtgtgg
     1441 gacgtcgccg acgacgacct cgatgcgctc gatacctacg agggacaccc gacgttctac
     1501 cgccgcgagc aggtgaccgt ggagacgtcg gacggtcccc tcgagtgcac gacgtaccgc
     1561 atcgcgcacc agcgcggcta cgtgcgcccg accgacgact acctctaccg catgcggtcg
     1621 gcgatccggg tccacgggct gccgcccgag gcactcgaca tcctcgaagc cgcggcgcgc
     1681 ccgcccggcc cgcacatctc gacctgacgc cggcgtgccg gacctgagct gggaccaggt
     1741 ccgtgcgtgg cgcgtcgcgc gtcacggcct cgacgaacga tcgacgagac ggcggctcct
     1801 gtccgtggcc tcggagatct gcggcgtcca cgctcaggtc ggctcggccg ccgagctgtc
     1861 gctgtgggcg cgcgtcgagg gcgtgacccc gagggacgtt cgcgacgcgc tgtggaagac
     1921 gaagcggctc gtgcgctcat ggatgatgcg cgggacgatc cacctgcttc ccgcgcgcga
     1981 cttcccgctc tacgtcgcgg cgctcgcgac gcacgaccgc tggtggaagg gcgcatggct
     2041 ccgcgccgtc gggatgtcgg cggacgagct cggggcgctc ctaggcgcga tccgcgaggc
     2101 gctgtccgac gagccgatca cgcgcgagca gctcgcgtcg cgcgtcggcc acctcgtcgg
     2161 cgaccacgcg cgcgagcatc tgctgtcggg gtgggggagc ctcctcaagc ccgccgcgtt
     2221 caacgggtcc ttgtgctccg gccccccgcg cggacagaac gtcacgttcg tccgccctga
     2281 tctctggctc ggccgctggg aggagcacga cggcgaccag gcggtgcgcg aggtgttccg
     2341 gcggtacctc cgcgcgtacg gtcagtcgca ccacgaggag ttcgcggcat ggtggggcgg
     2401 cgcgccggcg ttcgcacgaa gggctcgtct ggcgatcgag gaagagctcc gggagatcga
     2461 cgtcgacgac atgaagcggt gggtgctgcg gcgtgacctc gccgcgatgc agcgcaccgc
     2521 tcccctgcgc ggatccgtgc ggctcctccc gcacttcgac gcgtacgtga gggggttccg
     2581 cccgc
//
LOCUS       DASZOB010000478         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_15515, whole genome shotgun sequence.
ACCESSION   DASZOB010000478 DASZOB010000000
VERSION     DASZOB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15515"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VG709_07470"
     CDS             complement(<1..494)
                     /locus_tag="VG709_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWC32952.1"
                     /translation="MAYDRIMVGTDGSETAQRAEEAAARLAKACEAQLLVVTAYEKPG
                     EAEGIVQRAESRAREQGLTEVRTQMQRGKATDVLVELSDQQDVDLLVLVDKGMSGGKR
                     FLVGSVAGSVAQHGRTDILIVRTTAIRPADRPWGEYRSVLIGTDGSSTADRATRKGLE
                     LARRA"
     gene            575..1180
                     /gene="coaE"
                     /locus_tag="VG709_07475"
     CDS             575..1180
                     /gene="coaE"
                     /locus_tag="VG709_07475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWC32953.1"
                     /translation="MLLVGLTGGLGAGKSTVARMLAERGAVVVDADDLARRALDPGHP
                     GYDRVVETFGDDILDASGLVDRTKLAEAVFADEGKRRALESIVHPEVFRLLAEEIERR
                     RDRDDIVVFDAPLIIETRFDEACDVVVVVAAPLEHQIERVLRDRGMSEAEARARIAAQ
                     ISPEQRAARADVVLANDGDLASLERQVGTLWERLGREAAGR"
     gene            1213..1926
                     /locus_tag="VG709_07480"
     CDS             1213..1926
                     /locus_tag="VG709_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWC32954.1"
                     /translation="MDVRAVFFDAGETLLYPHPSFAEVFTEVLRDEGYTVDPARVQEI
                     VTAYSQRFTDTVVSGEGKLWSTSSERSRAMWRGIYDGFLTAVGIDEDHDRLFEALYTR
                     FTDLATYRLHPDALPVLERLRKAELTLGLISNFEEWLERLLVALEVGDFFDVTVISGV
                     EGVEKPDPEIFRLALDRAGVRAEESVYVGDHPFFDVDAAREAGLVPVLIDRRGRHEDV
                     DAIRITSLEDLPAAIDVDA"
     gene            1970..2488
                     /locus_tag="VG709_07485"
     CDS             1970..2488
                     /locus_tag="VG709_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWC32955.1"
                     /translation="MGKRGIGHLDEVLDNLGVFRAGAEELVLLCPSGFRRVPISQGDG
                     YYFSVQALAPEQVAFAHTHPDSEEWSVVLQGRGEALIRGTVPLDTGSVLGRAAAYPHG
                     FRTAEDPLYLLSIQMPRPGEGMTTWDEPGTTTDPIRCRQGGWCRRCPRCGGHSALVRR
                     GSFECENCELEF"
     gene            complement(2517..>3062)
                     /locus_tag="VG709_07490"
     CDS             complement(2517..>3062)
                     /locus_tag="VG709_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32956.1"
                     /translation="SATPRVSAVAGAGQLGEAFWFVRRGPRRADLFLAGAVRSGGAAI
                     FGRPAAGAGAFGFVARATFRRERRREFHCRGRGIGREIDPTQFEVDPLLRSAKLTMQA
                     RGTTHVVRWTGRGEAPDPDLHGEFGNVYFFSAADLDRSADARGRLFDRFVRARTNSYA
                     YLDEGAGGFFFVRPGVGPFRF"
BASE COUNT          427 a         1088 c         1100 g          447 t
ORIGIN      
        1 gcgcggcgcg cgagctcgag ccctttgcgg gtggctcggt cggcggtcga agagccgtcg
       61 gttccgatca gcacgctccg gtactcgccc cacggccggt cggcgggacg gatcgccgtc
      121 gtccgtacga tcaggatgtc ggtccggccg tgctgggcga cgcttccggc gacgctgccg
      181 acgagaaacc gcttgccgcc cgacatcccc ttgtcgacga gcacgaggag atcgacgtcc
      241 tgctggtccg acagctccac gagcacgtcg gtcgccttcc cgcgctgcat ctgcgtccgg
      301 acctcggtca ggccctgctc gcgcgcccgg gactccgccc gctgcacgat accctcggcc
      361 tcgccgggct tctcgtacgc ggtcacgacg aggagctgag cctcacacgc cttcgcgagc
      421 cgtgctgcgg cctcctcggc tcgctgcgcg gtctccgagc cgtcagtgcc caccatgatg
      481 cggtcgtacg ccatcaggtg ccgcatcata ccggcggcgg tggaatgcgc cgtcgcgcgt
      541 cactacgatc cacgcgttcg acagagggag gaccgtgctg ctggtcgggc tcaccggcgg
      601 gctcggcgcc gggaaatcga cggtcgcgcg catgctcgcg gagcgtggcg ccgtcgtcgt
      661 cgacgccgac gacctggcgc gtcgagcgct cgacccggga catcccggat acgaccgcgt
      721 cgtcgagacc ttcggcgacg acatcctcga cgcttccggg ctcgtcgacc ggacgaagct
      781 cgccgaggcg gtgttcgcgg acgagggaaa gcgccgagcg ctggagtcga tcgtccaccc
      841 cgaggtgttc cgcctgctcg ccgaggagat cgagcgccgg cgcgaccgcg atgacatcgt
      901 cgtgttcgac gcgccgctca tcatcgagac gcggttcgac gaggcgtgcg atgtcgtcgt
      961 cgtcgtggcg gcgccgctcg agcatcagat cgagcgcgtc ctccgagacc gcgggatgag
     1021 cgaggccgag gcgcgcgcac gcatcgccgc ccagatctcg cccgagcagc gcgcggcgcg
     1081 cgccgacgtc gtcctcgcga acgacggcga ccttgcgtcg ctcgagcggc aggtcgggac
     1141 gctctgggag cggctcggcc gcgaggcggc cggccggtag agcgatccac cggcgtgggg
     1201 aacatcgcgg ccgtggacgt ccgcgccgtg ttcttcgacg ccggcgagac gcttctgtac
     1261 ccacatccct cgttcgcgga ggtgttcacc gaggtgctcc gcgacgaggg ctacacggta
     1321 gatcccgccc gcgtccagga gatcgtcacg gcttactcgc agcggttcac cgacacggtg
     1381 gtctcgggcg agggcaagct ctggtcgacc tccagcgagc gatcgcgcgc gatgtggcga
     1441 gggatctacg acggcttcct gaccgccgtg gggatcgacg aggaccacga ccggctcttc
     1501 gaggcgctgt acacgcggtt caccgacctc gccacgtacc gactccaccc cgacgcgctt
     1561 ccggtgctcg agcgcctgcg gaaggctgag ctcacgctcg ggctgatctc gaacttcgag
     1621 gagtggctcg agcggctgct tgtggcgctc gaggtcgggg acttcttcga cgtcacggtc
     1681 atctccggcg tggagggcgt cgagaagccc gacccggaga tcttccggct ggccctcgac
     1741 cgcgccggcg tccgggcgga ggagtcggtg tacgtcggcg accacccctt cttcgacgtc
     1801 gacgccgcga gggaggcggg actcgttccc gtgctgatcg accggcgggg acggcacgag
     1861 gacgtcgacg cgatccgcat cacgtcgctc gaggacctcc cggccgcgat cgacgtcgac
     1921 gcctgagggc ctcttgcgcg ctgcggtcgg cgcggctttg attgtgcgga tggggaagcg
     1981 ggggatcggc cacctcgatg aggtcctcga caacctcggg gtgttccgtg ccggggccga
     2041 ggagctcgtg ctgctctgcc cttcggggtt ccgtcgcgtc ccgatctcgc aaggcgacgg
     2101 ctactacttc tcggtccagg cgctcgcgcc cgagcaggtc gcgttcgccc acacccatcc
     2161 cgattcggag gagtggagcg tcgtgctgca ggggcgcggc gaggcgctga tccgcggcac
     2221 ggtcccgctc gacaccgggt ccgtgctcgg gcgggcggcc gcgtacccgc acggcttccg
     2281 gacggcggag gacccgctct acctgctctc gatccagatg ccgcgcccgg gcgaagggat
     2341 gacgacctgg gacgagcccg gcacgacgac cgacccgatc cggtgccggc agggcggctg
     2401 gtgccgccgc tgcccgcggt gcggcgggca cagcgcgctc gtgcgtcgag ggtcgttcga
     2461 gtgcgagaac tgcgagctgg agttctgagc cgctagcggg agagccggac gtcgcgctag
     2521 aacctgaacg gcccgacgcc cggacgcacg aagaagaacc ccccggctcc ctcgtcgagg
     2581 tacgcgtagc tgttcgtcct cgcacggacg aagcggtcga acagccgccc ccgcgcgtcc
     2641 gcgcttcgat cgagatcggc cgcagagaag aagtagacgt tgccgaactc gccgtggagg
     2701 tcggggtcgg gggcctcgcc ccggccggtc cacctcacga cgtgggtggt cccgcgagct
     2761 tgcatcgtga gcttcgccga gcgcaggagc gggtcgactt cgaactgcgt gggatcgatc
     2821 tcgcgtccga tcccccgccc ccggcagtgg aactcccgcc ggcgttcgcg ccggaaggtc
     2881 gcgcgagcga cgaacccgaa agcgccggca ccggcggcgg gccgaccgaa gatcgcggcg
     2941 ccgccactcc ggacggcgcc ggccaggaac agatccgctc gccgcggacc ccgccggacg
     3001 aaccagaacg cctcgccgag ctggccggcg ccggcgaccg cggagacccg cggcgtcgcc
     3061 ga
//
LOCUS       DASZOB010000479         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_35613, whole genome shotgun sequence.
ACCESSION   DASZOB010000479 DASZOB010000000
VERSION     DASZOB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35613"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1530
                     /gene="cysS"
                     /locus_tag="VG709_07495"
     CDS             136..1530
                     /gene="cysS"
                     /locus_tag="VG709_07495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWC32957.1"
                     /translation="MRLFNTLTNQVEELVPVEQGLVKIYACGPTVYRYVHIGNLRTFL
                     LSDLLRRALEFEGHDVLLIMNITDVGHMTDDVLDAGEDKMDVSVARERLSPEQIAEKY
                     TAAFLEDCDAIGILRADQYPKATDHIAEMIALIERLIERGHAYVVGGSVYFDVHSFPG
                     YGRLSGNTLDNLRAGHRDIERDPNKRQPEDFALWKEAGPRRLMTWQSPWGVGFPGWHI
                     ECSAMSMRYLGDRFDVHTGGNDLKFPHHEDEIAQSDGATGHQVISTWVHGGHLRSSGQ
                     KMAKSTGNVTRIGDVVESLGVDPLAFRYLCFQTRYRSEMDFSLEAMLVADRSVKRMRQ
                     RMADWATEARNGLATEAKELDARFRDALADDLDLPEAVVVLNETLSAPIPGGEKYVLL
                     SAWDAVLGLDLERDARGGELVLPAEVRRLLEDRDAARAARDFARSDAIRDELTQLGYE
                     VMDTPEGTRVRARA"
BASE COUNT          251 a          571 c          561 g          220 t
ORIGIN      
        1 gcgcgctcgc gcgcgaaccg ccggaccgag gagacgatct cgcgctgctg atcgtcgagg
       61 ccgaagtccc ggtccacgag cgcggagcgt agcaccggct ccggagcgct ccgcccccgc
      121 gggtatcctt gcccgatgcg cctgttcaac acgctgacga accaggtcga ggagctcgtc
      181 ccggtcgagc agggcctcgt gaagatctac gcctgcggcc ccacggtgta tcgctacgtg
      241 cacatcggca acctgcgcac gttcctgctg agcgacctgc tccgccgggc gctcgagttc
      301 gagggtcacg acgtcctgct gatcatgaac atcaccgacg tcgggcacat gacggacgac
      361 gtgctggacg ccggcgagga caagatggac gtgtccgtcg ctcgggaacg gctctccccg
      421 gagcagatcg ccgagaagta cacggccgcc ttcctcgagg actgcgacgc gatcggcatc
      481 ctgcgggccg accagtaccc gaaggcgacc gaccacatcg ccgagatgat cgcgctgatc
      541 gaacggctca tcgagcgcgg ccacgcctac gtcgtcgggg gcagcgtcta cttcgacgtg
      601 cactcgttcc cgggctacgg gcgcctctcg ggcaacaccc tcgacaacct tcgggcgggg
      661 caccgcgaca tcgagcgcga cccgaacaag cggcagcccg aggacttcgc gctctggaag
      721 gaggccggtc cccgccgcct gatgacgtgg cagagcccgt ggggcgtcgg cttccccggg
      781 tggcacatcg agtgctccgc gatgtcgatg cgctacctcg gcgaccgctt cgacgtgcac
      841 accgggggca acgacctcaa gttcccacac cacgaggacg agatcgcgca gtcggacggg
      901 gcgacgggcc accaggtgat ctcgacctgg gtgcacggcg ggcacctgcg ctcgtccggc
      961 cagaagatgg cgaagtcgac cggcaacgtc acgcgcatcg gtgacgtcgt cgagtcgctc
     1021 ggcgtggacc cgctcgcgtt ccggtacctg tgcttccaga cgcgctaccg cagcgagatg
     1081 gacttctccc tcgaggcgat gctcgtcgcc gaccgatcgg tcaagcggat gcgccaacgc
     1141 atggccgact gggctaccga ggcgcggaac ggcctggcga cggaggccaa ggagctcgac
     1201 gcccggttcc gcgacgcgct cgccgacgac ctcgacctgc cggaggccgt cgtggtgttg
     1261 aacgagacgc tgtccgcccc gatccccggg ggcgagaagt acgtgttgct ctcggcctgg
     1321 gacgcggtgc tcgggctgga cctcgagcgc gacgcgcgcg ggggcgagct ggtgctcccg
     1381 gcggaggtcc gccgcctcct ggaggatcgt gacgccgccc gcgcggcgag ggacttcgcg
     1441 cgctcggacg ccatccgcga cgagctcacc cagctcgggt acgaggtcat ggacaccccc
     1501 gaggggacgc gggtccgcgc ccgcgcctag cgagcctcag ccgatcgcgg cgcgcaacgt
     1561 cgcctcggcg ccggccgcgc cgggatggcc gccgatcggg gcg
//
LOCUS       DASZOB010000480         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_177925, whole genome shotgun sequence.
ACCESSION   DASZOB010000480 DASZOB010000000
VERSION     DASZOB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177925"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..781
                     /locus_tag="VG709_07500"
     CDS             50..781
                     /locus_tag="VG709_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HWC32958.1"
                     /translation="MTDAPARAAEAEALEVLATGDLDVIGLLPHASNYTFLAEVSLGS
                     RSVRVVYKPRDGEMPLWDFRVGTLCRREVAAYVLAKALGWPNVPQTVLRDGPMGAGSV
                     QLFVGHDPREHYFTLRDRWTDAFREVAAFDVVVGNGDRKSGHCLAGDDGRIWAVDHGL
                     CFNVEPVLRTVIWDFAGHAVPRALLDDVARIAGELDDGRMRMALLELLDPEEVDATSR
                     RARALVEAGTFPVPGLGRSRPWPAI"
     gene            complement(1217..>2212)
                     /locus_tag="VG709_07505"
     CDS             complement(1217..>2212)
                     /locus_tag="VG709_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32959.1"
                     /translation="RRGDYPGASIDDVDEDALRRTLGKDAVRDLRRLKEVERALERAG
                     LVTRSGGRLEVTPRGARKLGERALVRVFERLRRDREGTHEAREAGGLAEPTGATRPWR
                     FGDAGQVAVQRSVFNAVVREGPGRAIRLAPDDLELVEAEARTETATALLLDLSFSMPL
                     RGHFVPAKRMALALHALIEGRYPTDRLYLIGFSDFARRMEPRDLTASAWERVYGTNMH
                     HAFNLAGRLLAQHPRAARQVIMVTDGEPTAHLDGEQSFFSWPPVPETIRRTLAEAIRL
                     ARAGVTINVFMLEDSPGLARFMERLARLTSGRVFLMSGESLGDFVLRDYVSRRAG"
BASE COUNT          298 a          803 c          790 g          321 t
ORIGIN      
        1 atccagacgg ccacatctgt cccgcgctga acgggcaggg ggagctcggg tgaccgacgc
       61 gccggcgcgt gccgcggaag ccgaggcgct tgaggtcctc gcaaccggcg atctcgacgt
      121 gatcggtctc ctgccgcacg cgtcgaacta cacgttcctc gccgaagtat cgctcggctc
      181 gcgcagcgtc cgcgtcgtct acaagccccg cgatggcgag atgccgctgt gggacttccg
      241 ggtcgggacg ctctgccggc gcgaggtcgc ggcctacgtg ctcgcgaagg cgctcggctg
      301 gccgaacgtc ccgcagaccg tgctgcgcga cggcccgatg ggcgcggggt cggtccagct
      361 gttcgtgggc cacgatccgc gcgagcacta cttcacgctc cgcgaccggt ggaccgacgc
      421 gttccgcgag gtcgcggcgt tcgacgtcgt cgtcgggaac ggcgatcgca agtccggaca
      481 ctgcctcgcg ggcgacgacg gcaggatctg ggccgtcgac cacgggctgt gcttcaacgt
      541 cgagcccgtc ctgcggaccg tgatctggga cttcgcgggc cacgccgtgc cgcgggcgct
      601 ccttgacgac gttgcgcgca tcgccggcga gctcgacgac ggccggatgc gcatggctct
      661 ccttgagctg ctcgaccccg aggaggtcga cgcgacgtcg cggcgcgcgc gcgctctcgt
      721 ggaggccggc acgttcccgg ttccggggct cggtcgctcc aggccctggc cggcgatctg
      781 atacgccgtc gagggcagcg gcggccgcgg tccgttcgcc tgacgacgaa gcggagcgcc
      841 tgccaggtct cgtcgatcga ggcggatttg ttgtcgacga gtccctcgtc gagcccagcc
      901 ctctgcacga tgtcgaacgc gaggtcggtc tcgaccctcg acgccttctt cggccaagcg
      961 acccacacgc cgccggtgtc ccggagctgg ttcgccaggc gcgggaaacg ccggcggacg
     1021 tcggcctcgc gcgtcacgaa cagcaccgcg acgtcgagat cctttctggg ccgtgcgtgg
     1081 atcgtcgcgc cgtcggggag cggggagagg cgctcctcga acccctcggg cgctccgacg
     1141 agcgcgacgt cgctccccgc tttgatcccg agcttctgga cgagcggcgt cgtggagtag
     1201 tcggttcgcc gccgcgtcac cctgcgcgcc tggagacgta gtcgcgcagc acgaagtcgc
     1261 cgagcgactc gccggacatg aggaagactc gtcccgaggt cagacgcgcg aggcgctcca
     1321 tgaaccgcgc gaggccgggc gagtcctcga gcatgaacac gttgatcgtg actccggcgc
     1381 gcgcgagccg gatcgcctcg gcgagcgtcc gccggatcgt ctcgggcacg ggaggccacg
     1441 agaagaacga ctgctcgccg tcgaggtgcg cggtcggctc gccgtcggtc accatgatca
     1501 cctgccgcgc ggcacgcggg tgctgggcga gcagccgtcc cgctaggttg aacgcgtggt
     1561 gcatgttggt gccgtacacg cgctcccacg ccgacgccgt taggtcgcgc ggctccatcc
     1621 gacgcgcgaa gtccgagaac ccgatgaggt acaggcggtc ggtcggataa cgcccctcga
     1681 tcagcgcgtg cagcgcgagc gccatccgct tcgccggaac gaagtgcccg cgcagcggca
     1741 tcgagaacga gagatcgagg agcagcgcgg tcgccgtctc ggtccgcgcc tcggcctcga
     1801 cgagctcgag gtcgtcgggc gcgagccgga tcgcgcgccc ggggccctcc cggacgaccg
     1861 cgttgaagac gctccgttgc accgcaacct gccccgcgtc accgaaccgc cacggccgtg
     1921 tggcgccggt cggctcggcg aggccgccgg cctcgcgcgc ctcatgcgtg ccttcccggt
     1981 cgcgccggag ccgctcgaag acacgcacca gcgcacgctc gccgagcttc ctcgcgccac
     2041 gcggcgtcac ctcgaggcgg ccgcccgagc gcgtgacgag ccccgccctt tcgagcgctc
     2101 gctccacctc cttgagccgc cggaggtcgc gaacggcgtc cttgccgagc gtccgccgca
     2161 gggcgtcctc gtcgacgtcg tcgatcgagg cgccgggata gtcgccgcgg cg
//
LOCUS       DASZOB010000481         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_137763, whole genome shotgun sequence.
ACCESSION   DASZOB010000481 DASZOB010000000
VERSION     DASZOB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137763"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VG709_07510"
     CDS             complement(<1..208)
                     /locus_tag="VG709_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006827634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWC32960.1"
                     /translation="MRPDDRCRDARPHPKPLGRLRYRAEETGFTYALISDHFHPWTDS
                     QGQSPFVWGTIGGIAQATERLRVGT"
     gene            146..925
                     /locus_tag="VG709_07515"
                     /pseudo
     CDS             146..925
                     /locus_tag="VG709_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
     gene            996..1265
                     /locus_tag="VG709_07520"
     CDS             996..1265
                     /locus_tag="VG709_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32961.1"
                     /translation="MLWGYTPIVVHRSFVVSTGGTLVLLPVRAVLFAIRLVEEHVAGR
                     SFDLSRSHGWIGLVAAAVGAAVVAAISLLVRSTARAVGARRRPTG"
     gene            1287..>2428
                     /locus_tag="VG709_07525"
     CDS             1287..>2428
                     /locus_tag="VG709_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWC32962.1"
                     /translation="MAKQTTQPTTAAPARPKAGTPRELSPHEAVNLFFDRAADHVGLE
                     DEMRAIFKTSYRELTVQVPVRMDDGTLDVFEGYRVQHNAARGPYKGGIRYHPDADLDE
                     VRALASLMTWKTAVLDIPFGGAKGGVRCDPTAMSADEKQKLTRRYTQMIGYVLGVNRD
                     IPAPDMNTDAQTMAWIMDAYSSRYGYTPAIVTGKPVELGGSYGREAATGRGVVYCLEE
                     AAKDLGIDLGGATVAIQGFGNVGSWAARLIGETGARVVAVSDVRGGVHAGGALDVQAL
                     FDHVRESGSVVGAPGTEEISNDDLIELDVDVLVPAALGGVIDQRNADRIAARVVVEAA
                     NHPVTPSGDDALQERGVAIIPDILVNAGGVTVSYFEWVQNIQQFRW"
BASE COUNT          378 a          829 c          872 g          349 t
ORIGIN      
        1 ccgtcccgac ccggagccgc tccgtcgcct gcgcgatccc gccgatcgtc ccccaaacga
       61 atgggctctg gccctggctg tccgtccatg gatggaagtg atcggagatg agcgcgtagg
      121 tgaagccggt ctcctcggcg cggtagcgga ggcgaccgag cggcttcggg tggggacggg
      181 cgtcacggca ccgatcatcc ggacgcaccc ggcgatcgtc gcgcacgcgt cggcgacgac
      241 cgcgacgatg atgccggggc ggttcttcct cggcgtagga accggggaga acctcaacga
      301 gcacatccac ggggacaggt ggccgtccat cgacatgcgg ctcgagatgc tcgaggaggc
      361 cgtgggtctc atccgcaagc tgtggaaggg tgggttcacg acccaccgcg gacgcttcta
      421 cacggtcgag caggcccggg tgtacgacgt tcccgacgac cctccaccgc tgtacgtcgc
      481 agcgaagggc cagcgcgccg cggagctcgc cgcgcgggtc ggagacgggg tcatcggcac
      541 ctctcccgac aaggagatgc tcgagcagtt cgacgacgcc ggcggcgccg ggaaggcgaa
      601 gatcggccag ctgcacgtct gctgggccca gaacgaggac gacgcgaaga agacagcgca
      661 cgagtattgg cggaacagcg cggtccccgg cgagctgtcg gtcgagctcc cgctcccaag
      721 gcacttcgag caggcgtcgc agctcgtcac gccggactcg ctcggcgagc agatgccggt
      781 cggcccggac gccgagcgct acaggaaggc gatctccgag tacgaggacg cgggctacac
      841 gcacgtgtac ctccatcaga tcggccccga ccaggagggg ttcttcggct tcgcggaacg
      901 ggagatcctc cccgccttcg gctaggttcc cgggcacgcg gtacgccgcg atcgcggccg
      961 ttgccggggc agtctgggga ctgatcgcgt acgtcgtgct ctggggctac accccgatcg
     1021 tggtccatcg atcgttcgtc gtgagcacgg gcgggacgct cgtcctgctc cccgtgcggg
     1081 cggtcctgtt cgcgatccgc ctcgtcgagg agcacgtcgc cgggcggtcg ttcgatctct
     1141 ctcgctcgca cggctggatc ggcctggtcg cggcggccgt gggcgcggcc gtggttgcgg
     1201 cgatctcact gctcgtgcgg tccaccgcgc gggccgtcgg tgcccggcgg cggccgaccg
     1261 gctaggctcc gcgggaggac cggaccgtgg cgaagcaaac gactcaaccg acgaccgcgg
     1321 cgccggcgcg accgaaggcc gggacgccgc gggagctgtc gccgcacgag gccgtcaacc
     1381 tcttcttcga ccgcgcggcc gaccatgtcg gtctcgagga cgagatgcgc gcgatcttca
     1441 agacctcgta ccgggagctc accgtgcagg tcccggtgcg catggacgac gggacgctcg
     1501 acgtgttcga gggctaccgc gtccagcaca acgccgcgcg aggtccgtac aagggcggga
     1561 tccgctacca ccccgacgcg gacctcgatg aggtccgagc cctcgcgtcg ctcatgacgt
     1621 ggaagacggc cgtgctcgac atcccgttcg gcggggcgaa gggcggcgtg cggtgcgacc
     1681 cgacggcgat gagcgccgac gagaagcaga agctcacgcg tcgctacacg cagatgatcg
     1741 gctacgtgct cggcgtcaac cgcgacatcc ccgcgcccga catgaacacc gacgctcaga
     1801 ccatggcgtg gatcatggac gcgtactcgt cacgctacgg ctacacgccc gcgatcgtga
     1861 ccgggaagcc cgtggagctc ggcggttctt acggacgcga ggccgccacc ggtcgcggtg
     1921 tcgtgtactg cctcgaagag gccgcgaagg acctcggaat cgatctcggc ggcgcgacgg
     1981 tcgcgatcca ggggttcggg aacgtcggct cgtgggcggc acggctgatc ggcgagaccg
     2041 gtgcgcgcgt cgtcgcggtc tccgacgttc gcggcggcgt gcacgccggc ggcgcgctcg
     2101 acgtgcaggc gctgttcgac cacgtccgtg agtcgggatc ggtcgtcgga gcgccgggga
     2161 ccgaagagat ctcgaacgac gacctcatcg agctcgacgt cgacgtcctc gtcccggccg
     2221 cgctcggcgg cgtgatcgat cagcggaacg ccgaccgtat cgcggcacgc gtggtcgtcg
     2281 aggcggcgaa ccacccggtg acgccctccg gcgacgatgc gctgcaggag cgcggcgtcg
     2341 cgatcatccc cgacatcctc gtgaacgccg gcggcgtcac cgtctcctac ttcgagtggg
     2401 tccagaacat ccagcagttc cgctggga
//
LOCUS       DASZOB010000482         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_178010, whole genome shotgun sequence.
ACCESSION   DASZOB010000482 DASZOB010000000
VERSION     DASZOB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178010"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..853)
                     /locus_tag="VG709_07530"
     CDS             complement(170..853)
                     /locus_tag="VG709_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWC32963.1"
                     /translation="MAVKALEVHKALADDTRFRLYRHLRLLGRPVSVREMSRRLALHP
                     NTLRPHLRRLEEAGLVAHQLQRTPGVGRPQTLYRAVEPSADDSRDFRLLAEMLCGLLV
                     SKRDRARATERAREWGQYLVSQGGPKPGARLPARQNLAALQDAMARAGFDPRFRRADG
                     AVQVTLRDCPFRELVDDYRDLVCSLHQGLIEGMLGGLRPGMALRDFRPLAERGICRLE
                     ARPADGMRP"
     gene            969..2123
                     /locus_tag="VG709_07535"
     CDS             969..2123
                     /locus_tag="VG709_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWC32964.1"
                     /translation="MVTREQVREALRDVLDPEIGKPIEDLGMLKDVEVSPDGDVRVHV
                     LLTIAGCPLRDRIDTDVRGAVEPLEGVRSVGVALGEMSGDQRQKMVTNLRGSQPGAAQ
                     APGHEGHGHPEIPAFFTDGDTQVIAVASGKGGVGKSSVTANLAAALAADGHRVGVLDA
                     DVWGFSIPRMMGVSGRPVAFNDMMLPLEAHGVKVISMGFFVGDDTPVIWRGPMLHKAL
                     QQFIGDVYWGELDYLLIDMPPGTGDVSLSLATFLPQGSMLVVTTPQDAASKVAERAGR
                     MAQHPNVKLQLLGVVENMSGFVCPHCGDVTDVFGTGGGEKTAGALGVPFLGGIPMQVS
                     LREGADTGRPAVVDEPDSPAGRALREVAHELARRTRTKVGKTLPLAVARR"
     gene            complement(2127..3155)
                     /locus_tag="VG709_07540"
     CDS             complement(2127..3155)
                     /locus_tag="VG709_07540"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWC32965.1"
                     /translation="MELLSVIGWPVIDRIRLGPVAISPHGIGIAAGYLAGAWWMLRHG
                     RRRGLSDDNMGTILFAALVGAIVGARFFYVVGHADEFESFVEVFQIWRGGISLVGGIV
                     GAIAFAYPYMRKFRYRFLQVMDSAAIGLAFGIVVGRIGDLLIGDHLGKPTDFFLGWAY
                     RGGTLPGPWVELPDAPGTWVACLADGSTQYLSRAGASLLPGEACPPIAALPQQMAAAG
                     VHQTALYDFFIAIGLFLFLLWMGRVARREGVLILTFAIWYGAGRVLTDFLRVDKTWFG
                     LTGSQWTSLGAIVISLIVLVRFARRPIVPPEGDEIDPETRAAAAEPVESVSRPTTEFT
                     PPREPGAG"
     gene            complement(3209..3304)
                     /locus_tag="VG709_07545"
                     /pseudo
     CDS             complement(3209..3304)
                     /locus_tag="VG709_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
BASE COUNT          522 a         1182 c         1207 g          420 t
ORIGIN      
        1 cacgttgatc gggacgctcg cgaccgagca gctccgtcgc cgtcgcggcg tcccggcgcc
       61 gaacagccac cgccggacgg ccctccgtcg ccgcgtcgtc cagcagctcg acgaggtgat
      121 cgtggggggc gacggcctca gggtcgtggt cgtgaggtag cgagaccggt caggggcgca
      181 tcccgtccgc ggggcgcgcc tcgagccggc agatcccgcg ctcggcgagc gggcggaaat
      241 cgcggagcgc catgccgggg cgcagcccgc ccagcatgcc ctcgatcagc ccctggtgca
      301 gggagcacac gaggtcgcgg tagtcgtcca cgagctcgcg gaacgggcag tccctgagcg
      361 tgacctggac cgctccgtcg gcgcgccgga acctgggatc gaagcccgcc cgagccatcg
      421 cgtcctggag cgccgcgagg ttctgtcgcg ccggcaggcg cgcgccgggc ttcgggccgc
      481 cctgggagac gaggtactgc ccccattccc gcgcgcgctc ggtcgcgcgc gcgcggtcgc
      541 gcttcgagac gaggagcccg cacagcatct cggcgagcag gcggaagtcg cgcgagtcgt
      601 cggcggaggg ctcgacggct cggtacagcg tctgcggccg gcccacgccg ggcgttcgct
      661 ggagctggtg cgcgacgaga ccagcctcct cgagccggcg gaggtgtgga cggagcgtgt
      721 tcggatggag cgccagacgc ctcgacatct cgcggaccga cacggggcgt ccgaggaggc
      781 gcaggtggcg atacagccgg aaccgcgtat cgtcggccag cgccttgtga acctcgaggg
      841 ccttgacggc cacgtgaggc ctcctcgccc ggcgcgcacg cccggcggat ttcgccggtg
      901 agcaccgtcc gaaacaggat aatatggagg ccgccgcgca tcggcgcggc ctctcatcgg
      961 aggaagcctt ggtcacccgg gaacaggttc gcgaagcgct gcgagacgtg ctcgaccccg
     1021 agatcggcaa gccgatcgag gacctcggca tgctcaagga cgtcgaggtc tcgccggacg
     1081 gcgacgtccg cgtccacgtc ctgctcacga tcgccggatg cccgctgcgc gaccggatcg
     1141 acaccgacgt gcgcggcgcc gtcgagccgc tcgagggcgt gcgctcggta ggcgtcgcgc
     1201 tgggcgagat gagcggcgac cagcgacaga agatggtgac gaacctccgc ggctcccagc
     1261 cgggcgccgc gcaggctccc ggccacgagg gccacggcca ccccgagatc ccggcgttct
     1321 tcaccgacgg cgacacgcag gtgatcgcgg tcgcttccgg caagggcggc gtcggcaagt
     1381 cgagcgtcac ggcgaacctc gcggcggcgc tcgcggcgga tgggcaccgc gtcggagtcc
     1441 tcgacgcgga cgtctggggg ttctcgatcc cgcgaatgat gggtgtctcg ggccgccctg
     1501 tcgcgttcaa cgacatgatg ctgccgctcg aggcgcacgg cgtgaaggtc atctcgatgg
     1561 ggttcttcgt cggcgacgac acgccggtca tctggcgcgg cccgatgctg cacaaggcgc
     1621 tgcagcagtt catcggggac gtgtactggg gggagctcga ctacctcctg atcgacatgc
     1681 cgccgggcac cggcgacgtg tcgctctcgc tcgcgacgtt cctgccgcag ggctccatgc
     1741 tcgtcgtcac gacgccgcag gacgcagcca gcaaggtcgc cgagcgcgcg gggaggatgg
     1801 cgcagcatcc gaacgtgaag ctccagctcc tcggcgtcgt cgagaacatg tccgggttcg
     1861 tctgccccca ctgcggcgac gtcaccgacg tcttcgggac gggaggaggc gagaagacgg
     1921 ccggggcgct cggcgtcccg ttcctcggcg ggatcccgat gcaggtctcg ctccgtgagg
     1981 gtgccgacac cggccgcccc gccgtggtcg acgagcccga ctcccccgcc ggccgcgccc
     2041 tccgcgaggt cgcgcacgag ctggcgcggc gcacgcgcac gaaggtcggg aagacgctcc
     2101 cgctcgccgt cgcgcgtcgc tgacggctaa ccggcgccgg gctcgcgcgg cggcgtgaac
     2161 tctgtcgtcg gccgcgacac cgactccacc ggctcggccg ccgcggcgcg cgtctccggg
     2221 tcgatctcgt cgccctcggg cggcacgatc ggtcttcgcg cgaaccggac gagcactatc
     2281 agcgagatga cgatcgcgcc gagcgacgtc cactggctcc ccgtgagccc gaaccacgtc
     2341 ttgtcgacgc gcaggaagtc cgtgaggacc cgtccggccc cgtaccagat cgcgaacgtg
     2401 aggatcagca cgccctcgcg gcgcgctacg cggcccatcc agagaaggaa caggaacagt
     2461 ccgatcgcga tgaagaagtc gtagagcgcg gtctggtgca ccccggccgc cgccatctgc
     2521 tgcgggagag cggcgatcgg aggacacgcc tcgccgggca agagcgacgc gcccgcgcgc
     2581 gacaggtact gcgtcgagcc gtccgcgaga cacgcgaccc aggtccccgg agcgtcgggc
     2641 agctccaccc acggtcctgg aagcgtcccg ccgcggtacg cccacccgag gaagaagtcc
     2701 gtcggcttgc cgagatggtc gccgatcaac aggtcgccga tccgtcccac cacgatcccg
     2761 aacgcgagac cgatcgcggc ggagtccatc acctgcagga accggtagcg gaacttccgc
     2821 atgtacgggt acgcgaacgc gatcgcaccc acgatcccac cgacgaggct gatgccgccg
     2881 cgccagatct ggaagacctc gacgaacgac tcgaactcgt cggcgtgtcc gacgacgtag
     2941 aagaaccgag caccgacgat cgcgccgacg agggcagcga acaggatcgt ccccatgttg
     3001 tcgtcggaga gcccgcgtcg cctgccgtgg cggagcatcc accacgcgcc ggcgaggtag
     3061 ccggcggcga tcccgatgcc gtggggcgag atcgcgaccg ggccgagccg gatccggtcg
     3121 atgacgggcc agccgatcac ggagaggagc tccacggcgg cgaagtctaa cgaagcacgg
     3181 tcggcgagac cgcggcgctc caggcgtcgg ccacggcgcg gcctagcagg aggccaccgg
     3241 cgacgtccgc ggggaagtgg acgccgaggt gcacgcgtga ccacgcgacg agaccggcga
     3301 gcgcggtcgc gccgaccgtg cttcgttaga c
//
LOCUS       DASZOB010000483         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_137963, whole genome shotgun sequence.
ACCESSION   DASZOB010000483 DASZOB010000000
VERSION     DASZOB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137963"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VG709_07550"
     CDS             <1..323
                     /locus_tag="VG709_07550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWC32966.1"
                     /translation="APGIQVSARALYERTARLLLVELVSPRSLIGKNTVEALQSGLLF
                     GHASMIDGMVERISKELGAPTVVATGGLASTVMEECNTIDHLEPWLTLEGLRIVFDRN
                     TERP"
     gene            320..1825
                     /gene="lysS"
                     /locus_tag="VG709_07555"
     CDS             320..1825
                     /gene="lysS"
                     /locus_tag="VG709_07555"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWC32967.1"
                     /translation="MTGENPAAPPADDVLTVRREKLDRLRGRGVDPFALRFDRDALAT
                     ELHERFGDLEAGASSNDTVRVAGRLVGLRRLGKLTFGVLRDSSGDIQLFLSDDTLTDE
                     AQALLEDLDLGDWIGAQGDVVRTKRGELSVAAAELTLLSKALRPLPEKWHGLRDVELR
                     FRQRYLDLATNREARERVMARARMLAALREHLEARGFVEVDTPILQAMPGGGLARPFV
                     THHEALDVPLYLRIAPELYLKRLLIGGLERVYEIGRNFRNEGIDREHEPEFTSLEAYQ
                     AYGTYEDMMELAEGLVKAGAEATLGSLTVEFRGTTLDLSKPWRRVPLAELVSEAVGER
                     VGLDTPAERLCDIAEAHEVRYDPEWSAGQLLAELYEKLVEETIVEPTFVKDFPKDVSP
                     LARPHRDDPRYTEHFDLVIAGIEMGPSYSELTDPDEQRERFEMQAALKSAGDEEAHPY
                     DDDFVLALEHGMPPAGGLGLGVDRLLMFLTDAPSLREVILFPTLRPERRGG"
BASE COUNT          288 a          695 c          695 g          290 t
ORIGIN      
        1 tcgcgcctgg gatccaggtc tcggcacgag cgctctacga gcggaccgct cggctgctgc
       61 tggtcgagct cgtctcgccg cggtccctca tcgggaagaa caccgtcgag gccctgcagt
      121 ccgggctgct gttcgggcac gcctcgatga tcgacggcat ggtcgagcgg atctcgaagg
      181 agctcggcgc cccgaccgtc gtcgccaccg ggggccttgc gtcgaccgtg atggaggagt
      241 gcaacacgat cgatcacctc gagccgtggc tcacgctcga gggactgcgg atcgtcttcg
      301 accggaacac cgagcgccca tgacgggcga gaacccggcg gctcctcccg cggacgacgt
      361 cctcaccgtt cgccgcgaga agctcgaccg gctccgcggc cgcggcgtcg acccgttcgc
      421 gctgcgcttc gaccgcgacg cgctcgcgac ggagctccac gagcggttcg gcgatctcga
      481 ggccggcgcc tcgtcgaacg acaccgtccg cgtggcgggg cgtctcgtcg ggttgcgccg
      541 gctcgggaag ctcacgttcg gggtcctgcg ggattcatcc ggcgacatcc agctgttctt
      601 gtcggacgac acgctgacgg acgaggctca ggcgctcctc gaggatctcg atctcggcga
      661 ctggatcggc gcgcagggcg acgtcgtccg gaccaagcgc ggtgagctgt cggtcgcggc
      721 ggcggagctc acgctgctgt cgaaagcgtt gcgcccgctc ccggagaagt ggcacgggct
      781 gcgagacgtg gagctgcggt tccggcagcg ctacctcgac ctcgcgacga accgcgaggc
      841 gcgcgagcgc gtgatggcgc gcgcgcggat gctcgcggcg ctgcgcgagc acctcgaggc
      901 ccgtggcttc gtcgaggtcg acacgccgat cctgcaggcg atgcccggcg gcggcctcgc
      961 gcgcccgttc gtgacgcacc acgaggcgct cgacgtcccg ctctacctgc gcatcgcgcc
     1021 ggagctctac ctgaagaggc tcctcatcgg agggctcgag cgcgtctacg agatcggccg
     1081 gaatttccgc aacgagggca tcgaccgcga gcacgagccc gagttcacgt cgctcgaggc
     1141 ctatcaggcc tacggcacgt acgaggacat gatggagcta gcggagggcc tcgtgaaggc
     1201 gggggcggaa gccacgctcg gctcgctcac ggtcgagttc cgtggcacga cgctcgacct
     1261 gtccaaaccg tggcgacgcg tcccgctcgc cgagctcgtg tccgaagccg tcggggagcg
     1321 cgtcgggctc gacacgccgg ccgagcgact gtgcgacatc gccgaggcgc acgaggtgcg
     1381 atacgaccct gaatggtcgg cgggacagct cctcgccgag ctctacgaga agctcgtgga
     1441 ggagacgatc gtcgagccga cgttcgtcaa agacttcccg aaagacgtgt cgccgctcgc
     1501 gcgtccgcat cgcgacgatc cccggtacac cgagcacttc gacctcgtga tcgcggggat
     1561 cgagatgggg ccctcgtact cggagctcac ggaccccgac gagcagcgcg agcgcttcga
     1621 gatgcaggcg gcgctgaagt cggctggcga cgaggaggcg catccatacg acgacgactt
     1681 cgtgctcgcg ctcgagcacg gcatgccgcc ggcgggcgga ctcgggctcg gcgtcgaccg
     1741 gctcctgatg ttcctcaccg acgcgccgtc gctccgcgag gtgatcctgt tcccgacgct
     1801 ccggccggaa cgtcggggcg gctgagcacg tcccagtgac cgtgcgccgc ctcctcgccg
     1861 tcgtcttcac gctcgccggc tgcgttgcca cgcccgccga cgacggagcg cccgtccgcg
     1921 gccccacgac cgacgagcgg ggcgacccca tcgtgccggt cgcggtcg
//
LOCUS       DASZOB010000484         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_188975, whole genome shotgun sequence.
ACCESSION   DASZOB010000484 DASZOB010000000
VERSION     DASZOB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188975"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VG709_07560"
     CDS             complement(<1..823)
                     /locus_tag="VG709_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32968.1"
                     /translation="MAPPLIPVHYVRRAAVSAALVAATGTAALYANRHLNGDIYWILA
                     AGRDVAENGVTETDPFLTLNHGREWRNQQWLTELVLYGLERLGGLRLVSLAYALVLGL
                     SVLPLLLGSRKRRISEALVAWTFLLPLLVAVLDPRAAGFSLLCFSLLIVLVDGERRRW
                     RVWLIPLLIALWANLHAAFLVGLLFFALVLAGAVVDRLLNRRADMFSVRFLLLAVALP
                     AALATPLALGIFDYLGTLSGNPLLPRLTFEWDPTVEHPLLLLVVVAFAGFAAHLFR"
     gene            complement(808..1527)
                     /locus_tag="VG709_07565"
     CDS             complement(808..1527)
                     /locus_tag="VG709_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWC32969.1"
                     /translation="MRPDAKRRIVVVPTYQEREALPRFLSEFDSTGFEALVVDDNSPD
                     GTGDWAETYSREHPWLHVLRRAGKDGLGSAYRAGFAWCLERDYGVIGQMDCDLSHPPE
                     ALARMRAALERADVVVGSRFCRGGRTIGWPWWRRVQSHAAPVPARLLLRLPVADLTGG
                     FKLWRADALRRIEVESTMSQGYVFQIETTYRAFRAGLRVLEVPIEFAERAAGESKMSG
                     AIKLEGVQVVLRLAREQWRPR"
     gene            1547..>1734
                     /locus_tag="VG709_07570"
     CDS             1547..>1734
                     /locus_tag="VG709_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32970.1"
                     /translation="MPEPAGAPRVVVVIVDGLPVELLERELPCLPFLRERLPYGAEAI
                     SCFPSTTGPAYFPFLAGAT"
BASE COUNT          276 a          597 c          639 g          222 t
ORIGIN      
        1 ggcggaagag gtgcgccgcg aagccggcga aggcgacgac gacgaggagc aggagcgggt
       61 gctcgacggt gggatcccac tcgaacgtga gcctcgggag cagcgggttt ccggagagtg
      121 tgccgaggta gtcgaagatg ccgagcgcga gcggcgtcgc gagcgcggcg ggaagcgcga
      181 ctgcgaggag caggaagcgg acgctgaaca tgtccgcgcg gcggttgagg agccggtcga
      241 cgaccgcgcc ggcgagcacg agcgcgaaga agagaaggcc gacgaggaag gccgcgtgca
      301 ggttggccca gagcgcgatc aggagcggga tcagccagac gcgccagcgg cggcgctcgc
      361 cgtcgacgag gacgatcaga agcgagaagc agagcaggct gaacccggcg gcgcgggggt
      421 cgagcaccgc gacgagcagc gggagcagga acgtccaggc gacgagcgcc tccgagatgc
      481 ggcgcttccg gctccccagc aggaggggaa gcacgctgag cccgagcacg agcgcgtacg
      541 cgagcgagac gagccgcaac ccgccgagcc gctcgagccc gtagaggacg agctcggtca
      601 gccactgctg gtttcgccat tcccgcccgt ggttgagcgt gaggaacggg tcggtctcgg
      661 tgacaccgtt ctccgcgacg tctcttcctg ccgcgaggat ccagtagatg tcgccgttca
      721 ggtgcctgtt cgcatagagc gcggccgtcc cggtcgccgc gacgagggcg gcgctcacgg
      781 ccgcccgcct cacgtagtgg acggggatca gcgggggcgc cactgctcgc gcgcgaggcg
      841 caggacgacc tgaactccct cgagcttgat cgcgccggac atcttcgact cgcctgccgc
      901 gcgctcggcg aactcgatcg ggacctcgag gacgcggagc cccgcccgga acgcgcggta
      961 cgtcgtctcg atctggaaga cgtacccctg ggacattgtc gactcgacct cgatccgccg
     1021 cagcgcgtcc gctctccaga gcttgaagcc tccggtcagg tcggcgacgg gcagccggag
     1081 gaggaggcgg gcgggaacgg gggcggcgtg gctctgcacc ctccgccacc acggccagcc
     1141 gatcgtcctc ccgccgcggc agaagcgcga gccaacgacg acatccgcgc gctcgagcgc
     1201 cgcgcgcatt cgcgcgagcg cctccggcgg atgcgagagg tcgcagtcca tctggccgat
     1261 gactccgtag tcgcgctcga ggcaccaggc gaagcccgcg cggtaggcgg atccgagccc
     1321 gtccttgccg gcgcggcgga ggacgtggag ccacgggtgc tcgcgcgagt aggtctccgc
     1381 ccagtcgccc gtgccgtccg gcgagttgtc gtccacgacc agcgcctcga accccgtgga
     1441 gtcgaactcg gagaggaacc tcggcagcgc ctcccgctcc tggtacgtcg ggacgacgac
     1501 gatccggcgt ttcgcatccg gcctcatcgc ctctcatact ttcaccgtgc cggagcccgc
     1561 gggcgccccg cgcgtcgtcg tcgtgatcgt ggacggcctt ccggtcgagc tcctcgagcg
     1621 cgagcttccc tgcctgccgt tcctgcgcga gcgcctcccg tacggagcgg aggcgatctc
     1681 gtgcttcccc tcgacgaccg ggccggcgta cttcccgttc ctcgccggcg cgac
//
LOCUS       DASZOB010000485         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_46733, whole genome shotgun sequence.
ACCESSION   DASZOB010000485 DASZOB010000000
VERSION     DASZOB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46733"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..780
                     /locus_tag="VG709_07575"
     CDS             325..780
                     /locus_tag="VG709_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32971.1"
                     /translation="MPSSARVLVVANRTAAPAALRAAVADRAARGPASFHLVVPANPH
                     GMHRVVDPDGAGREEAAARLEEALPMLEEAAGSEVTGEVGTADPLAAIMDAVHHRGFD
                     EIILSTLPRRVSRWLRVDLPSKARGLGLPVTHVEVPDAGIPTEDSLAAV"
     gene            996..>1878
                     /locus_tag="VG709_07580"
     CDS             996..>1878
                     /locus_tag="VG709_07580"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HWC32972.1"
                     /translation="MAVEGTRPIVLGVVGDSAAGKTTITRGLVRILGEDNVNHIATDD
                     YHRYDRQQRAERGITPLHPDCNYIDIMTQHLVHVRNGEPIMKPVYQHSDGTFGAPEYV
                     VPKRFAVIEGLLGYHTQELRDCYDVRVYLAPPEDLRRKWKVRRDCSRRGYTTDQVLEE
                     LDRREPDSAEFIRPQERRADLVVSFVPSESGDSTMLDAELVLREGLPHPDLSPITGDG
                     EQGITVTERRDERSLRIPSDIDSERAEEIEAIVWDRMHFATHLRTERLGEFTVGTYLQ
                     RSNSLAIVQVLILYHLVT"
BASE COUNT          303 a          648 c          649 g          278 t
ORIGIN      
        1 ggcttaggaa gccgccgagc ccgaccgggc tcgtcggctc ctcgacgtcg atgggcgacg
       61 agaccgccgc gaaagacgta gacgtgccgg gcgtctcctt cggcgagcag cgctgtgtcg
      121 aagccgcgcg cgacctcgtg aacctcgacg atgatgatcg cgtagctcgt ctgctccatc
      181 gagttacccg tcgaaagcgc gccgccgccg gccgtgcctg gtgagcgata ggacaggcgg
      241 cgaacagagc tcgagccgac gcgtggtatc acgctgcgct agtgttagtg acaatccgca
      301 catccgcgag aaggaggaga ccaaatgccc agctccgcca gggtgctcgt cgtcgccaac
      361 cgcaccgccg ccccggcggc gctgcgggcc gcggtagcgg accgtgccgc tcgcgggccg
      421 gcgagcttcc atctcgtggt gcccgcgaat ccccacggga tgcatcgcgt cgtcgatccg
      481 gatggcgcgg gtcgcgagga ggcggcggcc cggctcgagg aggcgcttcc gatgctcgag
      541 gaggcggccg gctccgaggt caccggggag gtcggtacag ccgatccgct cgcggcgatc
      601 atggacgcgg tccaccaccg cggcttcgac gagatcatcc tctctacgct gccgcgccgc
      661 gtctcgcgct ggctgcgggt ggacctgccg agcaaggcac gagggctcgg actcccggtc
      721 acgcacgtcg aggtgccgga cgccgggatt ccgacggagg attcgctcgc cgcagtctga
      781 gcgagcggtc cctggtcggg cgacggccag cccggcgggg ggcgggggct cgcctagctg
      841 ccgcgtcgtc ggctctgagg tcggagcgag ccgttcttgg cttgtagcgg caggtggtct
      901 accatgccgc ctccgatggg cgccggtgtc cccgactcta cgaccatttc ccgcgatcgc
      961 gagggcgagc acgcccgcgc gggcggtggt gtctgatggc cgtcgagggc actcgtccga
     1021 tcgtcctcgg cgtcgtcggc gactcggcgg ccggaaagac gacgatcacc cgcggcctgg
     1081 tgcgcatcct cggggaggac aacgtcaacc acatcgccac ggacgactac caccgctacg
     1141 accgccagca gcgcgcggag cgcggaatca cgccgctcca tccggactgc aactacatcg
     1201 acatcatgac ccagcatctc gtccacgtcc gcaacgggga accgatcatg aagccggtct
     1261 accagcactc ggacggaacc ttcggcgcgc cggagtacgt cgtgccgaag cgcttcgcgg
     1321 tgatcgaggg actgctcggc taccacaccc aggaactgcg cgactgctac gacgttcgcg
     1381 tctacctcgc ccccccggag gacctgcggc gcaagtggaa ggtcaggcgc gactgctcgc
     1441 ggcgaggcta cacgaccgac caggtactcg aggagctcga ccggcgagag cccgattccg
     1501 ccgagttcat ccgaccgcag gagcgcaggg ccgacctggt cgtctcgttc gtgccttccg
     1561 agagcgggga ctcgaccatg ctcgacgccg agctcgtcct gcgcgagggc ctgccgcatc
     1621 cagatctctc gccgatcacc ggcgacggag agcagggaat cacggtaacc gagcggcgtg
     1681 acgagcggtc cctgcggatc ccgagcgaca tcgacagcga gcgcgcggag gagatcgagg
     1741 cgatcgtctg ggacaggatg cacttcgcga cccatctgcg taccgagcgg ctcggtgagt
     1801 tcacggtcgg cacctacctt cagcgctcga actccctggc gatcgtgcag gtgttgattc
     1861 tctaccacct cgtgaccg
//
LOCUS       DASZOB010000486         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_189005, whole genome shotgun sequence.
ACCESSION   DASZOB010000486 DASZOB010000000
VERSION     DASZOB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189005"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VG709_07585"
     CDS             complement(<1..401)
                     /locus_tag="VG709_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWC32973.1"
                     /translation="MLAVLLGAAGFVALLLPGVAATVWMTGRIERRLDRLEREIDLTF
                     PRFPERSTIYARDGRRLATLFLEENRKVVRLRRVNEVTRKAVLAIEDSDFYEHDGIDR
                     RAVVRALVANVAEGRIEQGASTITQQLARNA"
     gene            572..778
                     /locus_tag="VG709_07590"
     CDS             572..778
                     /locus_tag="VG709_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32974.1"
                     /translation="MTDDQQDRDEGRREESEYPKAPGASKMEALERIERSLERQSEAT
                     LDRSDDAREDEEQPERDAAGDSDE"
     gene            863..>2804
                     /locus_tag="VG709_07595"
     CDS             863..>2804
                     /locus_tag="VG709_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HWC32975.1"
                     /translation="MTDPRARLVLLSFLMLFVELVLIRWTAANVVYLAFFTNFVLLAS
                     FLGIGIGFLRANLERDGFLLAPLALGLFVGFVFLFPVQWYAVGRRSGHFGLPPLPQWI
                     ELPVIFLGAAAVMAVIAEGVARTFATFEPLEAYRLDIVGSLLGIVGFSVLSFLHAPPI
                     AWAAVILLVFWVLLGRRIDPLPWGALGIVVIVFALQSFLPNVWWSPYYKVNTTDPDHE
                     GTIRVRVNNLAHQSIHPLDLLRDIQPFYYWTYEMLEGHPLRDVLIVGAGTGNDVALAL
                     SQGAERVVAVEIDPVLQDIGSEQHPDRPYQDPRVEVHIDDGRAYLERTDERFDLILFA
                     LPDSLTLVAGQGALRLESYLFTREALARVRDRLRPDGAFSAYNYYSPVVFRRYAHTLE
                     LVFGHPPCIRQGGALEGRRQWVMVIGQEAGSVRCPSTPVSIRNPPEPATDDHPFPYLR
                     ERGIPTYHLIALALILAASVVLVRLAGGRLSRMSSYLDLFFMGTAFLLLETKSIVQFA
                     LLFGTTWFVNALVFAGVLLAVLAAIEVARRFRLPAPWVLYLVLFAALVVAWLVPARAL
                     LALPLGLRFLAGTVITFAPIFLANLVFSQRFKDTASSVTAFGANLLGAMLGGVLEYGS
                     LVIGFRALVIGVAALYGLAYLFG"
BASE COUNT          381 a          992 c          938 g          493 t
ORIGIN      
        1 atagcgttcc gcgcgagctg ctgcgtgatc gtcgacgccc cctgctcgat ccgtccttcg
       61 gcgacgttcg cgacgagggc acggacgacc gcgcgtcgat cgatgccatc gtgctcgtag
      121 aagtcggagt cctcgatcgc caggacggcc ttcctcgtga cctcgttcac gcggcggagg
      181 cggacgacct tccggttctc ctcgaggaac agcgtcgcga gccggcggcc gtcccgcgcg
      241 tagatcgtcg agcgttcggg gaaccgcggg aacgtcaggt cgatctcgcg ctccagacgg
      301 tcgagccggc gctcgatgcg ccccgtcatc cagacggtcg cggcgacgcc cggcaggagc
      361 agggcgacga acccggcagc gccgagcagc accgcaagca cgagcgctag cccgcgcgcc
      421 ggtacgcgat gtggagagcg agatagggaa gccataggaa cggcgccgag aggcgcgtca
      481 tccatctatc tacccgcggg cggggaaccg gaatcgactc ggcccccgtc cggcgggttt
      541 ccggagctcc gcacgtggga acggcgctcg tgtgaccgac gaccagcagg atcgcgacga
      601 agggcgacgc gaggagtccg agtacccgaa ggcgcccggc gccagcaaga tggaggcgct
      661 cgagcgcatc gagcgatcgc tggagcggca atcggaggcc acgctcgacc gttcggacga
      721 cgcccgagag gacgaagagc agcccgagcg ggatgcggcg ggagattccg acgagtaacg
      781 ggtagtgtcg cgcgcgagcg tggcacgcga cactcgagag cccaaggccg ccccgccgcc
      841 gtcacgagac gaatcgccgc tcgtgacgga tccgcgtgcg cgcctcgtcc tgctcagctt
      901 cctgatgctg ttcgtggagc tcgtcctgat caggtggacg gccgcgaacg tcgtctacct
      961 cgccttcttc acgaacttcg tgctgctggc gagcttcctc ggcatcggca tcgggttcct
     1021 gcgcgcgaac ctcgagcggg acgggttcct cctcgcccca ttagcgctcg ggctgttcgt
     1081 cgggttcgtg ttcctgttcc cggtccagtg gtacgccgtc ggccggcgct ccgggcactt
     1141 cgggctgccg ccgttgccgc agtggatcga gcttccggtg atcttcctcg gcgcagccgc
     1201 cgtgatggcg gtcatcgccg aaggggtggc ccgcaccttc gccacgttcg agccgctcga
     1261 ggcgtaccgg ctcgacatcg tcggcagcct gctcggcatc gtcgggttct cggtgctgtc
     1321 gttcctccac gcgccgccga tcgcgtgggc cgccgtcatc ctcctcgtgt tctgggtgct
     1381 gctcggccgc cggatcgacc cgcttccgtg gggggctctc gggatcgtgg tgatcgtgtt
     1441 cgcgctgcag tcgttccttc cgaacgtctg gtggtcgccc tactacaagg tcaacacgac
     1501 ggacccggac cacgagggga cgatccgcgt ccgcgtcaac aacctcgcgc accaatcgat
     1561 ccatccactc gacctgctgc gtgacatcca gccgttctac tactggacgt acgagatgct
     1621 cgaagggcat ccgctccgtg acgttctcat cgtcggtgcc gggaccggca acgacgtcgc
     1681 gctcgcgctc tcacaggggg cggagcgggt ggtcgcggtc gagatcgatc cggtgctcca
     1741 ggacatcggg agcgaacagc accccgaccg gccgtaccag gatcctcgcg tggaggtcca
     1801 catcgacgac ggccgcgcgt acctcgagcg caccgacgaa cgcttcgacc tgatcctgtt
     1861 cgcgcttccc gactcgctca cgctcgtggc gggacagggg gcgctgcggc tcgagagcta
     1921 cctcttcacg cgcgaggcgc tcgcgcgggt ccgtgatcgc ctgcgccccg acggcgcgtt
     1981 ctccgcttac aactattact cgccggtcgt cttccgccgg tacgctcaca cgctggagct
     2041 cgtcttcggc caccccccgt gcatccggca gggcggcgcg ctcgaaggac gccgccagtg
     2101 ggtgatggtg atcgggcagg aggccgggag cgtccggtgc ccgtcgacgc ccgtctcgat
     2161 ccggaacccg ccggagccgg cgaccgacga ccatccgttc ccgtacctgc gcgagcgcgg
     2221 gatcccgacg taccacctca tcgcgctcgc gctcatcctc gcagcctcgg tggtcctggt
     2281 gcgcctcgcc ggcggccgcc tctccaggat gtcgtcgtac ctcgacctct tcttcatggg
     2341 gacggcgttc ctcctactcg agaccaagag catcgtgcag ttcgcgctgc tgttcggcac
     2401 gacgtggttc gtcaacgcgc tcgtcttcgc cggggtcctg ctcgccgtcc tcgcggcgat
     2461 cgaggtcgcg aggcggttcc gtctccccgc gccgtgggtg ctctacctcg tgctgttcgc
     2521 ggcgctggtc gtcgcgtggc tggtcccggc acgcgcgctg ctcgcgctgc cgctcgggct
     2581 caggttcctt gccggtaccg tgatcacgtt cgcgcccatc ttcctcgcga acctcgtgtt
     2641 ctcccagcgg ttcaaggaca cggcgtcgtc ggtcacagcc ttcggcgcga acctcctcgg
     2701 cgccatgctg ggaggcgtcc tcgagtacgg gtcgctggtc atcgggttcc gtgccctcgt
     2761 gatcggagtc gcggccctct atgggctcgc gtacctattc ggcc
//
LOCUS       DASZOB010000487         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_46830, whole genome shotgun sequence.
ACCESSION   DASZOB010000487 DASZOB010000000
VERSION     DASZOB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46830"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2335
                     /locus_tag="VG709_07600"
     CDS             <1..>2335
                     /locus_tag="VG709_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HWC32976.1"
                     /translation="VEDWQPMRQRALEIVAALESEPPPVDAEEASEARALLEWILDDN
                     FSFLGYREYELAAEDGEDILRGRPGTGLGLLRDAKAVSRSFAKLTPEARRLARAPTLL
                     TLTKANSRSTVHRPAYLDYIGVKHFDASGEVVGERRFLGLYTSTAYRARPRDIPILRR
                     KVDRVLERSGLRPGSHDHKDLLDILETYPRDELFQISEDDLFEISRGILHLGERKRVR
                     LFVRRDAYGRFVSCLVFVPRERYTTETRVRMQEILQDAFGGTSSDFAVRLSESTLARV
                     HFVIRTQPGSDAAPDVAEIEQRLVQVTRAWADDLLEALVEELGEERGVALFRKYADAF
                     PPAYRSDFSPRMAVPDIERIEALDPAGDVAMSLYRPLEASAGTLGFKLVRSGSPILLS
                     DILPHLENMGVQVSDERPYEIRPKGADGVWIYDFGLVHEDEGQLEAGRVREAFQEAFA
                     RTRHGEAESDGFNRLVLGARLAWRDIALLRALCKYLRQTRMPYSQGYMEQAVSAHPEI
                     ARRLVELFHVRFDPAREGARADDARRVSEEAAELVDAVRSLDEDRILRSLLDLVQATL
                     RTNYFQTGSTGEPKPCFAFKLDPSRVPDLPLPRPKFEIFVYSPRTEGVHLRGGKVARG
                     GIRWSARREDFRTEVLGLMKAQTVKNAVIVPVGAKGGFVVKNPPADGDRDALQAEVVA
                     CYRTLISGMLDLTDNIVEGEVVPPPDVVRYDDDDPYLVVAADKGTATFSDVANSIAAE
                     YGFWLGDAFASGGSTGYDHKAMGITARGAWESVERHFR"
BASE COUNT          343 a          849 c          808 g          335 t
ORIGIN      
        1 gtcgaggact ggcagccgat gcgccagcgg gcgctcgaga tcgtcgccgc gctcgagtcg
       61 gagccgcctc ccgtcgacgc cgaagaggcg agtgaggcga gagcgctcct cgagtggatc
      121 ctggacgaca acttctcgtt cctaggctac cgcgagtacg agctcgccgc ggaggatggg
      181 gaggacatcc tgcgcggccg gcccggcacc gggctcgggc tcctgcgcga tgccaaggcg
      241 gtgtcgcgca gcttcgcgaa gctcacgccg gaggcgcgcc ggcttgcgcg cgcgccgacg
      301 ctcctcacgc tgacgaaggc gaactcgcgc tcgacggtgc accggcccgc gtacctggat
      361 tacatcgggg tgaagcactt cgacgcatcg ggcgaggtcg tcggcgagcg tcgcttcctc
      421 ggcctctaca cgtcgacggc gtaccgggcg cggccgcgcg acatcccgat cctccggcgc
      481 aaggtcgacc gcgtgctcga gcgctcgggc ctgcgaccgg ggagccacga ccacaaggac
      541 ctcctcgaca tcctcgagac gtatccgcgc gacgagctct tccagatctc cgaggacgac
      601 ctcttcgaga tctcgcgcgg gatcctccac ctcggggaac ggaagcgcgt tcgcctcttc
      661 gttcggcgcg acgcctacgg ccgcttcgtc tcgtgcctcg tgttcgtgcc gcgcgagcgc
      721 tacacgacgg agacgcgcgt ccgcatgcag gagatcctcc aggacgcctt cggcgggacg
      781 agcagcgact tcgcggtccg gctcagcgag tcgaccctcg cgcgcgtgca cttcgtgatc
      841 cgcacccagc ccggcagcga cgcggccccg gacgtcgccg agatcgagca gcggctcgtg
      901 caggtgacgc gcgcctgggc cgacgatctc ctcgaggcgc tcgtcgagga gctcggcgag
      961 gagcgcggcg tcgcgctctt ccggaagtac gccgacgcgt tcccgcccgc ctaccgctcg
     1021 gacttctccc cgcggatggc ggtgccggac atcgagcgga tcgaggcgct ggatccggcc
     1081 ggcgacgtcg ccatgagcct ctaccggccg ctcgaggcgt ctgcgggcac gctcggattc
     1141 aagctcgtcc ggtcgggttc gccgatcctc ctctcggaca tcctcccgca cctcgagaac
     1201 atgggcgtgc aggtctcgga cgagcggccg tacgagatcc ggccgaaggg cgcggacggc
     1261 gtctggatct acgacttcgg cctcgtccac gaagacgagg gccagctcga ggccggacgc
     1321 gtgcgcgagg cattccagga ggccttcgcg cggacgcggc acggcgaggc cgagagcgac
     1381 ggcttcaacc ggctcgtgct cggggcgcgc ctcgcgtggc gcgacatcgc cctcctgcgc
     1441 gccctctgca agtacctccg ccagacgcgg atgccctaca gccagggcta catggagcag
     1501 gcggtctcgg cgcacccgga gatcgcgcga cgtctcgtcg agctcttcca cgtccgcttc
     1561 gatccggcac gcgaaggggc ccgggcggac gacgcgcgcc gcgtgtccga ggaggccgcc
     1621 gagctcgtgg acgcggtccg gagcctcgac gaggaccgga tcctccggag cctcctcgac
     1681 ctcgtccagg cgacgctccg gacgaactac ttccagaccg gatcgaccgg cgagccgaag
     1741 ccgtgcttcg cgttcaagct cgacccttcg cgcgtgcccg acctgccgct cccgcgcccg
     1801 aagttcgaga tcttcgtcta ctcgccgcgc acggaggggg tgcatctccg cggcggcaag
     1861 gtcgcgcgcg ggggcatccg ctggtcggcc cggcgcgagg acttccgcac ggaggtgctc
     1921 ggcctcatga aggcccagac ggtcaagaac gccgtcatcg tgcccgtggg cgcgaagggg
     1981 ggcttcgtcg tcaagaaccc tcccgcggac ggagaccgag acgcgctcca ggccgaggtc
     2041 gtcgcctgct accggacgct catctccggg atgctcgacc tcaccgacaa catcgtcgag
     2101 ggcgaggtcg tcccgccgcc cgacgtcgtc cgctacgacg acgacgaccc ctacctcgtc
     2161 gtcgcggccg acaaggggac ggccacgttc tcggacgtcg cgaactcgat cgcggccgag
     2221 tacggcttct ggctcggcga cgccttcgcc tccggcgggt ccacgggcta cgaccacaaa
     2281 gcaatgggca taaccgcgcg cggcgcctgg gagtcggtgg agcgtcactt ccggg
//
LOCUS       DASZOB010000488         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_178450, whole genome shotgun sequence.
ACCESSION   DASZOB010000488 DASZOB010000000
VERSION     DASZOB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178450"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VG709_07605"
     CDS             complement(<1..255)
                     /locus_tag="VG709_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC32977.1"
                     /translation="MIEVPIADVNGVRLAYDDEGSGTPLMLVHGSWTNRRGWDAVAGK
                     LAERFRVVRLDRRGHSGSEAASGTLEDDAADVAALAASLDV"
     gene            complement(252..>1598)
                     /locus_tag="VG709_07610"
     CDS             complement(252..>1598)
                     /locus_tag="VG709_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32978.1"
                     /translation="LVGGRRDAPARQRTLRAAIDWSYDLLEEDARSFFRRLSVFTGGC
                     TFEAAGEVADPHGELGDVMDLLDALLQHSLLLRDPDPGDARFRMLETIREFGVERLEE
                     IGEGDRVRERHALRFVALVEEAEPHLTGPEQRRWIEVLNREHDNLRAALAWAIEADRG
                     EIALRLGGALWRFWYGQGFLAEGRRWLDSVLALPSSSERSLARVKGLTALGGIAYWQN
                     DFGPSDAAYVEAVDIVRELGDAGAMPETLFNMAMTKAVMGEPERAMSLLEESLERARQ
                     LGDRRREAWALWGLSAGSMFGGDLDRAVTLSSETLRLFEEIRDDTWGLGNALAGQAGL
                     AVMRGEPEVGRERVLQALEAWEDQGNALVIASQLQFLGIAEIRLGRPERAVRLAGASA
                     ILREKVGGKVPDAFFPFEDPHETAAKGRDPETLERLWTEGRAMSVHEALAYARDET"
BASE COUNT          238 a          608 c          518 g          234 t
ORIGIN      
        1 gacgtcgaga gacgcggcga gcgccgccac gtcggcagcg tcatcttcga gcgttccgct
       61 tgccgcctcg ctcccgctgt gaccgcgacg atcgagcctg acgacccgga aacgctcggc
      121 gagcttcccg gcgacggcgt cccagccacg gcggttggtc cacgacccgt ggacgagcat
      181 gagcggcgtc ccggatccct cgtcgtcgta cgcgagtcgg acgccgttca cgtccgcgat
      241 cggcacctcg atcacgtctc gtcgcgggcg taggcgagcg cttcatgcac ggacatcgcg
      301 cggccctcag tccacagccg ctcgagcgtc tcggggtcgc ggcccttcgc ggcggtctcg
      361 tgcggatcct cgaacgggaa gaaggcgtcc ggcaccttgc cgccaacctt ctctcggagg
      421 atcgccgaag caccggcgag gcgcacggcg cgctccggcc ggccgagccg gatctctgcg
      481 atcccgagga actggagctg cgacgcgatc acgagcgcgt tcccctggtc ctcccaggcc
      541 tcgagcgctt ggagaacacg ctcccgcccc acttcgggct cgccgcgcat caccgcgagt
      601 cccgcttgac ccgcgagcgc gttgccgagg ccccacgtgt cgtcccggat ctcctcgaac
      661 agccggagcg tctccgagct cagcgtgacg gcgcggtcga ggtcgccgcc gaacatgctc
      721 ccggcgctga ggccccagag cgcccacgcc tcgcgacggc gatccccgag ctgccgggcg
      781 cgctcgaggc tctcctcgag cagcgacatc gccctctcgg gctctcccat gacggccttg
      841 gtcatcgcca tattgaagag cgtctccggc atggcgcctg cgtcgcccag ctcccgcacg
      901 atgtcgacgg cctcgacata ggccgcatcc gacggcccga agtcgttctg ccagtaggcg
      961 atgcccccga gtgccgtgag gcctttcacg cgtgcgaggc tccgctcgga gctcgatggg
     1021 agcgccagga ccgaatccag ccatcgacga ccttctgcga ggaagccctg cccgtaccag
     1081 aaccgccaca gcgcaccgcc gagacgaagg gcgatctctc cccggtccgc ctcgatcgcc
     1141 cacgcgaggg ccgcgcggag gttgtcgtgc tcgcggttga gcacctcgat ccagcggcgt
     1201 tgttccggcc cagtgaggtg cggctccgcc tcctcgacga gcgcgacgaa ccgaagcgcg
     1261 tggcgctcac gtacgcggtc tccctcgcca atctcctcga gccgctcgac cccgaactcg
     1321 cggatcgtct cgagcatgcg gaaccgcgcg tcgccggggt caggatcgcg cagcagcagg
     1381 ctgtgctgaa gcaacgcgtc gagcagatcc atcacgtcgc cgagctcccc gtgcggatcc
     1441 gcgacttctc cagccgcctc gaacgtgcac ccgcccgtga agacgctcag gcggcggaag
     1501 aacgatcgag cgtcctcctc gagcaggtcg tagctccagt cgatcgccgc gcgaagcgtt
     1561 cgctggcgcg ccggcgcgtc gcggcgaccg ccaacgag
//
LOCUS       DASZOB010000489         8342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_16338, whole genome shotgun sequence.
ACCESSION   DASZOB010000489 DASZOB010000000
VERSION     DASZOB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16338"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VG709_07615"
     CDS             complement(<1..121)
                     /locus_tag="VG709_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWC32979.1"
                     /translation="MPWVRSITFGVWVSIGSSDESPRMLGASHFLEHLLFKGTK"
     gene            complement(199..2526)
                     /locus_tag="VG709_07620"
     CDS             complement(199..2526)
                     /locus_tag="VG709_07620"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWC32980.1"
                     /translation="MADTMQGIRVTRNIGGKDVTFESGTLAKLAAGSVVVTAGETQVL
                     VTCTSGPAREGIDFFPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTARLIDRPLR
                     PTFRKGFRQEVHVVCTVLSADLANPYDIPGINGAGVAAMLAALPFEGPVAGVRLALAG
                     GRWLVNPTYQEADLGTFDMVVAGRRNDQGGVDILMVEAEAPETALDHIADGGTAPTEE
                     VVAEGLEEAKRVIGEIIDLQREFMEAVGGAEREFPMDADVDEEIQRAIDEFARGRLSE
                     ALQIAEKAQRNEAVDRVREEMETHLSGLWEEEAYQGRANVVARVFKDLQKDVMRRRVV
                     DAGVRMDGRKTDELRPISCAVGLVPRAHGSGLFQRGETQVLNVTTLGMLRMTQQIDTL
                     DPDDTKRYIHHYNFPPFSTGETGFMRGPKRREIGHGALAERALLPVIPSEEDFPYAIR
                     LVSDVLMSNGSTSMASVCASTLSLMDAGVPIKAPVAGIAMGLIAEGGEFVTLTDILGA
                     EDALGDMDFKVAGTKDFVTALQLDTKVTGLPTEVLVGALEQARAARLQILDKMLEAIS
                     EPREVPSEKAPRVMTKQIPVDKIGEVIGPKGKRINEIIAVTGADIDIQDDGTVFIGAR
                     EGEGAERASQMIDEILNPRMPEIGEVFHGKVVKTTTFGAFVNILPGTDGLVHISELRK
                     GGERVQTVEDVVNIGDELDVVVLKIEQGLKPKIGLRPIWEGETPPTVDEINAEQAAKE
                     SRPPRDRDRDRDGGERRGDRGDRGRRRPPRRREEA"
     gene            complement(2688..2954)
                     /gene="rpsO"
                     /locus_tag="VG709_07625"
     CDS             complement(2688..2954)
                     /gene="rpsO"
                     /locus_tag="VG709_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWC32981.1"
                     /translation="MLAKEEKHRVISEYRRQDRDTGSPEVQVAVLSKRIDELTEHLRT
                     HKSDHHSRRGLLQLVGRRRRLLEYLKREDIERYRTLIGRLGLRR"
     gene            complement(3004..3930)
                     /locus_tag="VG709_07630"
     CDS             complement(3004..3930)
                     /locus_tag="VG709_07630"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWC32982.1"
                     /translation="MEVLRALDDVPDGHASAVTIGFFDGVHRGHRAVIGRTVHVARER
                     GLRSVVVTFDRHPLETLSPGKVPSLLTTLEQKAELIDELGVDALFVLGFTEEVSRWSP
                     EGFVDRVLARGLEARFVIVGTNFTFGHRAAGNLPVLADLGQANGFDVEGMGINKLDGR
                     PVSSTAIREALVEGDLTWAERALGRRFAVRGRVVAGAGRGANLGFPTANLETPPGMLL
                     PGMGVYAGRAFVDGDAWIAAINIGVNPTFGRDPLHLEAYLLGFDGDLRGRPIEVEFWA
                     RLRDEQRFASAEALSRQIAEDVERTKEIVPTS"
     gene            complement(3976..4821)
                     /gene="truB"
                     /locus_tag="VG709_07635"
     CDS             complement(3976..4821)
                     /gene="truB"
                     /locus_tag="VG709_07635"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWC32983.1"
                     /translation="MTHGLLLVDKPSGVTSHDVVERVRRAIGVRKVGHAGTLDPMATG
                     LLVLGVGRATRLLRFLGDLEKEYEGTARLGEVTDTLDADGQIVRTSPVDVEEAAVRDA
                     MAAFHGDILQRPPAYSAVKVGGRRMYAAARKGEELEAELRSVRVDAFELVSFDGRHAG
                     FRIVCSSGTYVRSLVADVGEKLGCGAHLARLVRTRIGLFHVRDARPPEDPGDPLPLER
                     AVAHLPRRDAEHDDEARAASNGRCLGPSGLDGPHALFDPDGRLVGIFRDTGTKSCPEV
                     VLPAG"
     gene            complement(4818..5807)
                     /locus_tag="VG709_07640"
     CDS             complement(4818..5807)
                     /locus_tag="VG709_07640"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWC32984.1"
                     /translation="MREALDEAARVLDEADDVTLVCHVNPDPDAMGSMLGLAIFLAGR
                     GKRVAASWPNDPAEPPRWLEALPGREHVVAPGKLPKQSDVLVVLDAADAGRLDGLTHL
                     LDRSRSVVVLDHHRTNPGFGTVNVVDGEASATAEIVFRLIDRMGGEPDADAATCLYAG
                     LVADTGRFQYEATSPEVLRIAARLRERSFDHARLAQALFEDNSVGYLRLLGRVLERAE
                     HVPEANLVWASVSRDDLQAAGIPIQETDDLMDVLRTAREADVAAVAKEQREGGYKVSL
                     RSRGDTDVAAVAESFGGGGHRLAAGYTSTVSLEETVRKLVDVLVAANPATAAP"
     gene            complement(5800..6156)
                     /gene="rbfA"
                     /locus_tag="VG709_07645"
     CDS             complement(5800..6156)
                     /gene="rbfA"
                     /locus_tag="VG709_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWC32985.1"
                     /translation="MTEGVRPERVSEEFREVFAEEIPKLKDPRVGFVTVTGVDVSPDL
                     RRARIFYTVMGEEADRRSTAAGLRSARGHLRAVLGRQIRLKFLPELDFEEDDSFERAK
                     RVDELLHKIREEDRDA"
     gene            complement(6149..6442)
                     /locus_tag="VG709_07650"
     CDS             complement(6149..6442)
                     /locus_tag="VG709_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWC32986.1"
                     /translation="MLVVLSSFDLRIPGCTSLKEKRHVVKALTAGLRGKFNVAVAEVD
                     HQDLWQRATIGVSAVAAQGYHLRKVMHEVEKYLSTWPAIEVIDHTMTLHAQDD"
     gene            complement(6468..>8342)
                     /gene="infB"
                     /locus_tag="VG709_07655"
     CDS             complement(6468..>8342)
                     /gene="infB"
                     /locus_tag="VG709_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWC32987.1"
                     /translation="RGGQVADIAVVVVAAEAPAPEPAPEPEPEPEPPPAEPTRPELRV
                     ARGISVKDLAERLGVPATELIAKMWGAGEMVTITQSLSDDAIKLLGGDYGYAVELVAP
                     EEEEQAVADDDEDVDPDRLRPRPPVVTVMGHVDHGKTLLLDAIRQTDVVSGEAGGITQ
                     HIGAYQVHQDGDVITFIDTPGHEAFTAMRARGAQATDIAVLVVAADDGVMPQTVEAID
                     HARAAGVPIVVAVNKVDKPEADPIRVRQQLAEQGIQTSEWGGDFEFVDVSAKAGTNLD
                     KLLETILVVAELQELKADPETRARGVAIEAHLDKGRGPVATVLVQRGTLRVGDAIVCG
                     TAQGRVRAMLDEHGGQLTEAGPARPVQILGWSKVPDAGDDFRVVEDEKEARRIAQERE
                     TRHRAADLVAAKPAFALEDLLEATSAGELSELNIVLKADTHGSLEAVADALERLSTKE
                     VAVRSIRGGVGAITESDVMLAQASQAIVLGFNVRPDPNAREIADREGVDVRLYRVIYQ
                     AVDDIKQALSGMLTPTKQESDLGRAEVRATFRVPRLGVVAGCYVTSGTILRGARARLL
                     RDGAVVYEGRIGSLRRFKDDVREVAEGFECGIGLENFQDVKEGDVIEAFEVREVARSL
                     "
BASE COUNT         1156 a         3057 c         2810 g         1319 t
ORIGIN      
        1 tcttggtccc cttgaacaag agatgctcga ggaagtggga ggcgccgagc atccgcggag
       61 actcgtcgct cgagccgatc gagacccaca cgccgaacgt gatcgagcgc acccacggca
      121 tgcgctcggt cacgagccgg agaccgctct cgtgctcgct gcgccggatg tccgagcggc
      181 cggcgcgacg tgaggggctc aggcctcctc ccgccgacgc ggaggccgcc ggcggcctcg
      241 atcgccacgg tcgccgcgcc gctcgccacc gtcccggtcg cggtcgcggt cgcgcggcgg
      301 gcggctctcc ttcgcggcct gttcggcatt gatctcgtcg acggtcggcg gcgtctcgcc
      361 ctcccagatc ggacggagtc cgatcttcgg cttcaggccc tgctcgatct tgagcaccac
      421 gacgtcgagc tcgtcgccga tgttcacgac gtcctcgacg gtctggacgc gctcgccgcc
      481 cttgcggagc tccgagatgt gcacgaggcc gtcggttccg ggaaggatgt tcacgaacgc
      541 gccgaaggtc gtggtcttca cgaccttccc gtggaacacc tcgccgatct cgggcatccg
      601 gggattcagg atctcgtcga tcatctgcga cgcgcgctcc gcgccctcgc cttcgcgcgc
      661 gccgatgaac accgtcccgt catcctggat gtcgatgtcg gctcccgtca ccgcgatgat
      721 ctcgttgatc cgcttgccct tcggcccgat cacctcgccg atcttgtcga ccgggatctg
      781 cttcgtcatg acccgcggag ccttctccga cggcacctcg cggggctccg agatcgcctc
      841 gagcatcttg tcgaggatct ggagccgtgc cgcgcgggcc tgctcgagcg ctccgaccag
      901 cacctcggtc gggagccctg tcaccttcgt gtcgagctgg agcgcggtga cgaagtcctt
      961 ggtccccgcg accttgaagt ccatgtcacc gagagcgtcc tcggcgccga ggatgtcggt
     1021 cagcgtcacg aactcgccgc cttccgcgat gaggcccatc gcgatgcccg ccaccggcgc
     1081 cttgatcggg acgccggcgt ccatgagcga gagcgtcgag gcacacacgc ttgccatcga
     1141 ggtcgagccg ttcgacatca gcacgtccga cacgaggcgg atcgcgtagg ggaagtcctc
     1201 ctccgacggg atcacgggga ggagcgcccg ctccgcgagc gcgccgtggc cgatctcgcg
     1261 ccgcttcggc cctcgcatga accctgtctc gccggtcgag aacggcggga agttgtagtg
     1321 gtggatgtag cgcttggtgt cgtcgggatc gagcgtatcg atctgctgcg tcatccggag
     1381 catcccgagc gtcgtgacgt tcaacacctg ggtctcgccg cgctgaaaga gcccggagcc
     1441 gtgcgcgcgc ggcacgagcc ccaccgcgca ggagatcggg cgcagctcat cggtcttgcg
     1501 cccgtccatc cggacgccgg cgtcgaccac gcggcgccgc atgacgtcct tctggaggtc
     1561 cttgaacacg cgcgcgacga cgttcgcgcg tccctggtac gcctcctcct cccacaggcc
     1621 ggacaggtgc gtctccatct cctcgcgcac ccggtccacg gcctcgttgc gctgtgcctt
     1681 ttcggcgatc tgcagcgcct cggagagacg tccgcgcgcg aactcgtcga tcgcccgctg
     1741 gatctcctcg tcgacgtcgg cgtccatcgg gaactctcgc tccgccccgc cgacggcctc
     1801 catgaactcg cgctggaggt cgatgatctc gccgatcact cgcttcgctt cctcgaggcc
     1861 ctcggcgacg acctcctcgg tgggcgcggt gccgccgtcg gcgatgtggt cgagggccgt
     1921 ctcgggcgcc tcggcctcga ccatgaggat gtcgacgccg ccctggtcgt tccgccggcc
     1981 ggcgacgacc atgtcgaacg tgccgagatc ggcctcctgg tacgtcgggt tcacgagcca
     2041 ccgaccaccg gcgagcgcga gacgcacgcc tgcgacgggg ccctcgaacg gaagcgcggc
     2101 cagcatcgcc gcgactccgg cgccgttgat acccgggatg tcgtacggat tcgcgagatc
     2161 cgcggacagg acggtgcaga cgacgtgcac ctcctggcgg aagcccttgc ggaacgtcgg
     2221 ccggagcgga cggtcgatga gccgcgcggt caggatcgcg gtctcgctcg ggcggccctc
     2281 ccggcggaag aagccaccgg ggatcttgcc ggccgcgtac atccgctcct cgacgtcgac
     2341 cgtgagcggg aagaagtcga tcccttcgcg tgccgggccg ctggtgcatg ttacgaggac
     2401 ctgcgtctcg ccggcggtca cgacgacgga gccggctgcg agcttcgcca gcgtcccgct
     2461 ctcgaaggtg acgtccttcc cgccgatgtt ccgggttacg cggatgcctt gcatcgtgtc
     2521 agccatctcg acctcctcgg tcgttccgag gagcggcggc ggggggccag ttgtcagtgg
     2581 tggaccacgg accgcggggt ccggagcccg ctactgataa ctgcccgctc gaaccgacct
     2641 ctccttcctt ctgcggttgt tctcttcctt gcttcccgcc ggggccgcta gcgccggagc
     2701 ccgaggcggc cgatcagtgt tcggtagcgt tcgatgtcct cgcgcttcag gtactcgagc
     2761 agccggcgac gacgcccgac cagctggagc agcccgcggc gggagtgatg gtcgctcttg
     2821 tgggttcgga ggtgctcggt cagctcgtcg atccgcttcg acagcaccgc tacctgaact
     2881 tcgggggatc cggtgtcgcg gtcctggcgg cgatattccg agatgacccg gtgcttctct
     2941 tccttagcaa gcatcgcgag ccagcgtagc acagggcctc gtggaccgac cgtgacgccg
     3001 atctcacgac gtcgggacta tctccttcgt ccgctcgacg tcttcggcga tctgacgaga
     3061 cagcgcctcg gcggaggcga acctctgctc atcccgaagg cgggcccaga actcgacctc
     3121 gattggacgc cctcggagat caccgtcgaa cccgagcagg tacgcctcca ggtgcagcgg
     3181 atcgcgtccg aaggtcgggt tcaccccgat gttgatcgcg gcgatccacg cgtcaccgtc
     3241 gacgaaggca cggccggcgt acacgcccat cccgggcagc agcatcccgg gcggcgtctc
     3301 gaggttcgcc gtcgggaacc cgaggttcgc gccgcggccg gcgcctgcca ccaccctccc
     3361 acgcacggcg aaacggcggc cgagcgctcg ctcggcccac gtgaggtcac cctccaccag
     3421 cgcctcccgg atcgccgtcg acgacaccgg tcgaccgtcg agcttgttga tccccatccc
     3481 ctcgacgtcg aagccgttcg cctgcccgag gtcggcgagg accggcaggt tgcctgcggc
     3541 gcggtgcccg aacgtgaagt tcgtgccgac gatcacgaac cgcgcctcga gcccgcgcgc
     3601 gagcacccgg tccacgaacc cctcggggga ccagcgcgag acttcctcgg tgaacccgag
     3661 gacgaacagc gcgtcgacgc cgagctcgtc gatcagctcc gccttctgtt cgagcgtcgt
     3721 gaggagcgac ggcaccttcc cgggcgacag cgtctcgagc ggatggcgat cgaacgtgac
     3781 gacgacggag cggagacctc gttctcgcgc gacgtggacg gtgcgcccga tcacggcccg
     3841 gtgcccgcga tggacgccgt cgaagaaccc gatcgtgacg gccgatgcat gaccgtccgg
     3901 cacgtcgtcg agagcccgca ggacctccac gccggtgagc gtagcgaagc cggccgcctc
     3961 tccaggggcg ggcggtcagc cggccggcag caccacctcc gggcaggact tggtcccggt
     4021 gtcgcggaag atcccgacga gccgaccatc gggatcgaac agcgcatgcg gcccgtcgag
     4081 tcccgacggc ccgaggcagc gcccgttgct cgcggcgcgc gcctcgtcgt cgtgctcggc
     4141 gtcgcgtcgc ggcaggtgcg cgaccgcgcg ctcgagcggg agtggatcgc ccggatcctc
     4201 gggcggtcgc gcgtcgcgca cgtggaacag cccgatcctc gtgcgcacga gccgcgcgag
     4261 gtgcgcaccg cacccaagct tctcaccgac atcggcgacg agcgagcgca cgtaggtccc
     4321 gctcgagcac acgatgcgaa agccggcatg gcgtccgtcg aacgagacga gctcgaacgc
     4381 gtcgacacgg acggaacgca gctcggcctc gagctcctcg cccttccgcg ccgcggcgta
     4441 catccgtcgc cccccgacct tcaccgccga gtacgccggc gggcgttgga ggatgtctcc
     4501 gtggaacgcc gccatcgcgt cacgcacggc cgcctcctcg acgtcgacgg gcgaggtgcg
     4561 gacgatctgg ccgtcggcgt cgagcgtgtc ggtcacctcg ccgagccggg cggttccctc
     4621 gtactccttc tcgaggtcgc cgaggaatcg caggagccgc gtcgcgcgcc cgacgccgag
     4681 cacgaggagt cccgtcgcca tcggatcgag cgtcccggcg tgtccgacct tccggacgcc
     4741 gatcgcgcgc cggacgcgct cgacgacgtc gtgcgacgtg acgcccgacg gcttgtcgac
     4801 gaggaggagc ccgtgggtca cggcgccgcc gtcgcggggt tcgcggccac gagcacgtcg
     4861 acgagcttcc tgacggtctc ctcgagcgaa acggtcgagg tgtagcccgc cgctaggcgg
     4921 tgtccgccgc cgccgaacga ctcggcgaca gcggcgacgt cggtgtcgcc gcgcgaccga
     4981 agcgagacct tgtacccgcc ctcccgctgc tctttcgcga cggccgcgac gtcggcctcg
     5041 cgcgcggtac ggaggacgtc catgaggtcg tcggtctcct ggatcgggat cccggccgcc
     5101 tggaggtcgt cgcgagagac cgatgcccac acgaggttcg cctccgggac gtgctccgcg
     5161 cgttcgagga cccgcccgag gagcctcagg tagccgacgg agttgtcctc gaacagcgcc
     5221 tgcgcgagtc gcgcatggtc gaacgagcgc tcgcggagcc tcgccgcgat gcgcaggacc
     5281 tccgggctcg tcgcctcgta ctggaagcgt cccgtgtcgg cgacgagccc cgcgtacagg
     5341 cacgttgccg cgtcggcgtc cggctcccct cccatgcgat cgatcagccg gaagacgatc
     5401 tcggccgtgg ccgacgcctc gccgtcgacc acgttcacgg tcccgaaccc cgggttcgtc
     5461 cggtggtggt cgagcaccac gaccgagcgc gagcggtcga ggagatgcgt gagcccgtcg
     5521 agccggccgg cgtcggcggc gtcgaggacg acgagcacat cggactgctt gggaagcttc
     5581 ccgggcgcca cgacgtgctc gcggcccggc agcgcctcga gccaccgcgg cggctccgcg
     5641 gggtcgttcg gccacgatgc cgcgactcgt ttcccccgac cggcgaggaa gatcgcgaga
     5701 ccgagcatgg accccatcgc gtccgggtcg gggttgacgt ggcagacgag cgtgacgtcg
     5761 tcggcctcgt ccaggaccct cgccgcctcg tcgagtgcct cacgcatcac ggtcctcctc
     5821 acggatcttg tgcagaagct cgtcgacgcg cttggcgcgc tcgaagctgt cgtcctcctc
     5881 gaagtcgagc tccggcagga acttcagccg gatctggcgc ccgaggaccg cgcggagatg
     5941 cccgcgggct gaccgcaagc ccgccgcggt gctccgacgg tccgcctcct cgcccatcac
     6001 cgtgtagaag atgcgcgccc gccgcaggtc cgggctcacg tcgacgccgg tcaccgtgac
     6061 gaacccgacg cgcgggtcct tcagcttcgg gatctcttcg gcgaacacct cgcggaactc
     6121 ctccgagacc cgctcgggcc gaaccccctc agtcatcctg tgcgtggagc gtcatggtgt
     6181 ggtcgatcac ctcgatggct ggccatgtcg aaaggtactt ctccacctcg tgcatcacct
     6241 tccgcaggtg atatccctgc gccgctacgg cggagacacc gatcgtggcg cgctgccaga
     6301 ggtcctggtg gtcgacctcc gccaccgcca cgttgaactt gccccgaagc cccgcggtca
     6361 acgccttcac gacgtggcgc ttctccttga gcgacgtgca ccctgggatg cggaggtcga
     6421 acgacgacag cacgaccagc atctcgtcct cctgcgcgcc gcgcggccta gaggcttcgg
     6481 gccacctcgc ggacctcgaa ggcctcgatg acgtcgccct ccttgacgtc ctggaagttc
     6541 tcgagcccga tgccgcactc gaagccctcg gcgacctcac gcacgtcgtc cttgaaccgc
     6601 cgcaacgacc cgatgcgccc ctcgtagacg accgcgccgt cgcggagcaa ccgcgcgcgc
     6661 gccccgcgca ggatcgtccc gctcgtcacg tagcacccgg cgacgacgcc gagccgcggc
     6721 acgcggaacg tggcgcgcac ctctgcgcgc ccgaggtcgc tctcctgctt cgtaggtgtg
     6781 agcatcccgg agagcgcctg cttgatgtcg tcgacggcct ggtagatcac gcggtacagg
     6841 cgcacgtcga cgccctcgcg gtcggcgatc tcacgcgcgt tcgggtcggg acggacgttg
     6901 aatccgagca cgatcgcctg cgacgcctgc gcgagcatga cgtcgctctc ggtgatcgcg
     6961 ccgacgccgc cgcggatgct gcgcacggcg acctccttcg tcgacagacg ctcgagggcg
     7021 tccgcgaccg cctcgagcga tccgtgggtg tcagccttga ggacgatgtt gagctcgctg
     7081 agctcgccgg cgctggtggc ctcgagcagg tcctcgagcg cgaacgccgg cttcgccgcc
     7141 acgaggtccg ccgcccggtg ccgcgtctcg cgctcctgcg cgatccgccg cgcctccttc
     7201 tcgtcttcga cgacgcggaa gtcgtccccc gcgtccggga ccttggacca gccgaggatc
     7261 tgcaccgggc gggccgggcc ggcctccgtg agctgcccgc cgtgctcgtc gagcatcgcg
     7321 cggacgcgcc cctgggcggt tccgcagacg atcgcgtcac cgacccgcag cgtcccccgt
     7381 tggaccagca cggtcgcgac gggcccgcgt cccttgtcga gatgcgcctc gatcgcgacg
     7441 ccgcgcgcgc gcgtctcggg atcggccttg agctcctgga gctccgcgac gacgaggatc
     7501 gtctcgagga gcttgtcgag gttggtccct gccttcgcgg agacgtcgac gaactcgaag
     7561 tcgccgcccc actccgacgt ctggatcccc tgctccgcaa gctgctgccg gacacggatc
     7621 gggtcggcct ctggcttgtc gaccttgttg acggcgacga cgatcgggac gccggccgca
     7681 cgcgcgtgat cgatcgcctc gacggtctgg ggcatcacgc cgtcgtccgc ggccacgacg
     7741 aggaccgcga tgtcggtcgc ctgggcgccg cgtgctcgca tcgcggtgaa cgcctcgtgg
     7801 cccggcgtgt cgatgaacgt gatgacgtcg ccgtcctggt ggacctggta cgcaccgatg
     7861 tgctgcgtga taccgccggc ctcgcccgag acgacgtcgg tctgccggat cgcgtcgagg
     7921 aggagcgtct tgccgtgatc cacgtgcccc atcaccgtca cgaccggcgg acgaggccga
     7981 aggcggtctg ggtcgacgtc ctcgtcgtcg tcggcgacgg cctgctcttc ctcctcgggc
     8041 gcgacgagct cgacggcgta tccgtagtcg ccgccgagca gcttgatcgc gtcgtcggag
     8101 agcgactgcg tgatcgtgac catctcgccg gcgccccaca tcttcgcgat gagctcggtc
     8161 gccgggacgc cgagccgctc cgcgaggtcc ttgacgctga tgccgcgcgc gacgcgcaac
     8221 tccggccgcg tcggctcagc cggaggcggc tccggctccg gctcgggttc gggcgcgggc
     8281 tcgggggcag gcgcctccgc cgcgaccacc accacggcga tgtcggcgac ctggccgccg
     8341 cg
//
LOCUS       DASZOB010000490         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_16385, whole genome shotgun sequence.
ACCESSION   DASZOB010000490 DASZOB010000000
VERSION     DASZOB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16385"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>1957
                     /locus_tag="VG709_07660"
     CDS             34..>1957
                     /locus_tag="VG709_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWC32988.1"
                     /translation="MTTPSVLAVIVVRDAAPWVRRSLAALARQTHPRLGVLAVDNAST
                     DGSADVLERLLGRRRVIRSERNLGFPGGVRRALSVPAAREADFVLLLHDDVALAPNAV
                     EAMVEAAGRFERAGIVGAKVVDWERPEVLREVGFASDRFGHPYSPLEEDEIDHGQYDA
                     PREVLFVSSAAMLVSRDVWQRVGEADERLRPAHGDLDLCWRARVAGFRVVVTPDAVAY
                     HRMAGAREERAERSGGEVSYVLERAVLAAMLKNYRAITLLWILPLYVAQSAIRATGAV
                     VSRRLDEVVGILRAWGWNLIRLPGTIRRRARAQAVRAASDREIARFMTPPGTRLRRLA
                     QQASALVSRYGGATAEEEEAEPEPLRRRAATILSAHPAAVALTVAAFLVLVGFRDVLF
                     ADAVSGGALPAFPAGASAFFEELARPWRSAGFGGSGAPSPAVAVLGIAGILTLGDPQL
                     LARLIVALGPLLAAASCFRAVSRVTRDSRAATVAAVAYGLSALALWTASKGRVAVVVL
                     LVILPWLGMRVGSRFDDPPPRSPLRWIVGTAAVLAIAGSFFPAVWLSTVVLVLPVAAS
                     AHGRRARAVTLSLGATAVAAILVFPFSSELVFAGRGTGVEAAGRAAFFALLRLSPEPA
                     VGSWLPALFLPLAGILG"
BASE COUNT          196 a          716 c          708 g          337 t
ORIGIN      
        1 ccggcgaccg cgtgaaatgc tatgccgccc ccagtgacga ccccttccgt cctcgccgtc
       61 atcgtcgtga gggacgccgc cccatgggtc cggcgttcgc tcgccgccct cgcgcggcag
      121 acgcatcctc gtctcggcgt gctcgccgtc gacaacgcgt ccaccgacgg gtctgccgac
      181 gtcctcgagc ggctgctcgg tagacgtcgg gtgatccggt ccgaacggaa cctcgggttc
      241 cccggtggcg tccgacgcgc gctctccgtg cctgcggcgc gcgaggccga tttcgtgctg
      301 ctgctccacg acgacgtggc gctcgcgccg aacgcggtcg aggcgatggt cgaggcggcc
      361 ggtcgcttcg agcgcgccgg gatcgtcggc gcgaaggttg tcgactggga gcggcccgag
      421 gtgctccgcg aggttggctt cgcgtcggac cggttcggac atccgtactc accgctagaa
      481 gaggacgaga tcgaccacgg tcagtacgac gcgccgcgcg aggtgttgtt cgtatcgtcc
      541 gccgcgatgc tcgtctcgcg cgacgtatgg cagcgcgtcg gcgaagccga cgagcggctc
      601 cgtcccgctc acggcgatct cgatctctgt tggcgtgcgc gcgtcgcggg gttccgtgtc
      661 gtggtgacgc cggacgccgt cgcgtaccac cggatggcgg gcgcgaggga ggaacgagcc
      721 gaacggagtg gcggcgaggt ctcgtacgtc ctcgaacgcg ccgtcctcgc cgcgatgctc
      781 aagaactacc gtgcgatcac gctcctgtgg atcctcccgt tgtacgtcgc gcagtcggcg
      841 atccgcgcca ccggcgccgt cgtctcgagg cggctcgacg aggtcgtcgg gatccttcgc
      901 gcgtggggat ggaacctcat ccggcttccg gggacgatcc gtcggcgcgc gcgtgcgcag
      961 gccgttcgag cggccagtga tcgcgagatc gcgcgcttca tgacaccgcc cgggacgagg
     1021 cttcgcaggc tcgcgcagca agcgtcggcg ctcgtctcgc ggtacggcgg cgcgacggcc
     1081 gaggaggagg aggcggagcc cgagccgctt cggcgccgtg ccgctacgat cctctccgct
     1141 cacccggccg ccgtcgcgct cacggtcgcg gcgttcctcg tcctcgtcgg cttccgcgac
     1201 gtgctgttcg cggacgcggt gagcggcggc gcgctgcccg ccttcccggc cggtgcgtcc
     1261 gcgttcttcg aggagctcgc gaggccgtgg aggtcggcgg ggttcggtgg gagcggcgca
     1321 cccagcccgg ccgtcgcggt gctcggcatc gcggggatcc tcacgctcgg cgacccgcag
     1381 ctcctggcgc gtctcatcgt cgccctcggc ccgctccttg ccgcggcgtc gtgcttccgc
     1441 gccgtctctc gcgtcacgcg tgactcccgc gcggcaacgg tagccgcggt cgcctacggg
     1501 ctctcggcgc ttgcgctatg gaccgcgtcg aagggccgcg tcgcggtcgt cgtgctcctc
     1561 gtcatccttc cgtggctcgg gatgcgcgtc ggctcacggt tcgacgaccc gccgccgcgc
     1621 tcgcccctcc gctggatcgt cggcacggcg gcggtgctcg cgatcgcagg ctcgttcttc
     1681 ccggccgtct ggctgtccac ggtcgtgctc gtgctcccgg tcgccgcctc cgcgcatggc
     1741 cggcgagcgc gggccgtgac gctgtcgctc ggcgccacgg ccgtcgctgc gatcctggtg
     1801 ttcccgttct cgagcgagct cgtgttcgcg ggccgcggga cgggcgtgga ggccgccggg
     1861 cgcgccgcct ttttcgcgct gctccgtctg tccccggaac cggccgtcgg aagctggctc
     1921 ccggcgcttt tcctgccgct agccgggatc ctcgggt
//
LOCUS       DASZOB010000491         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_138504, whole genome shotgun sequence.
ACCESSION   DASZOB010000491 DASZOB010000000
VERSION     DASZOB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138504"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..892
                     /gene="murI"
                     /locus_tag="VG709_07665"
     CDS             65..892
                     /gene="murI"
                     /locus_tag="VG709_07665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWC32989.1"
                     /translation="MPDPRPLGVFDSGVGGLTVVRAIIDQLPGESLVYYGDTARAPYG
                     DRGLHEIRAFAREIATELLRRDVKMLVVACNAIEVAAIEDLTAEAGVPVVGVIEAGTR
                     AALRATRNGRVGVIGTRATIESGAYERAVARERDGVELFSQACPAFVPYVERGDTTSE
                     DLLDIAHGYLAPLVAASIDTLVLGCTHYPLISGLLQVVMGPDVLLISSAEETAKDVYA
                     SLVREDRLRDDDVPPAHEFLCSGDPGSFLALGGRFLGPEVVEVRAARVDASPVPERP"
     gene            889..1641
                     /locus_tag="VG709_07670"
     CDS             889..1641
                     /locus_tag="VG709_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC32990.1"
                     /translation="MILTVLGAHGTWPLAGGATSGYLVRHDGFSLWIDCGSGTMANLQ
                     KHVGMFDVDAVAISHVHPDHLVDLYAYFFARVYSPEKPPKRPLFTPPGLLDQFVVMLQ
                     DTAMEEMGGTFDLVTVEPGETFEAGPFRVDTRPMTHPVPTLGMRIAADGAVLAYTADT
                     GPTDEIVPLASGADLLLAEATWQDDHEEAAPIHLTARQAGEIGARVEARRLMLTHLRP
                     HLDRDKSQDDASSSFPGEVSLAAEDFSIEVGR"
     gene            1638..2357
                     /gene="rph"
                     /locus_tag="VG709_07675"
     CDS             1638..2357
                     /gene="rph"
                     /locus_tag="VG709_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWC32991.1"
                     /translation="MTRTDGRAPDELRPIRYDVGYLDWAEGSVLLEVGRTRVLCSASW
                     EPRVPGFLHGSRKGWVSAEYSMLPRATQERTPREASRGRPSGRTQEIQRLIGRALRGI
                     VRLEKLGENTIWVDCDVLQADGGTRTASITGGYIALALALRSLKERGVVREDVLVDSV
                     AAVSAGIVGDERLLDLTYEEDSRAEVDLNVVMTGSRRLVEIQGTAEGQPFSRDDLDAL
                     IAFSERGIDRLTAVQREAAGA"
     gene            2361..2969
                     /gene="rdgB"
                     /locus_tag="VG709_07680"
     CDS             2361..2969
                     /gene="rdgB"
                     /locus_tag="VG709_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWC32992.1"
                     /translation="MAFPERLVIASRNPGKIREIRSICADWPVAWLTHGELEWPEVEE
                     TGESYLENALLKARSVAGATGEAALADDSGIEVDALGGAPGPRSARYAGEEASDEANL
                     RALIRAVAGVPAGGRTARYRAVAVVAWPGGRELTGEGACEGVLGTKPRGSGGFGYDPI
                     FEPMGAGRTMAELSPEEKDRISHRGRALRALRTQLEGSEAPG"
     gene            3158..>3684
                     /locus_tag="VG709_07685"
     CDS             3158..>3684
                     /locus_tag="VG709_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC32993.1"
                     /translation="MAAVPQEITEKDAKRVEAFQPTDVDDLRLYLEAAGREPLLSKQE
                     EVELAMAIEHGKEAEEKLRRGRIKSDRTKMRLREDIRKAERARRRFILANLRLVVSIA
                     KKYIGRGMSFLDLIQEGNIGLIRAVEKFDYEKGFKFSTYATWWIRQAITRAIADHSRT
                     IRIPVHMVETINRLM"
BASE COUNT          570 a         1250 c         1350 g          514 t
ORIGIN      
        1 ggcctggacc gctgacatcg agcaggccgc gaagcgtgtc gaggagacgc tctggtggtg
       61 actcgtgcct gacccacgtc cgctcggcgt cttcgattcg ggcgtcggcg ggctcaccgt
      121 ggtgcgcgcg atcatcgacc agctcccggg cgagtcgctc gtgtactacg gcgacaccgc
      181 tcgcgcgccc tacggtgatc gcgggctcca cgagatccgc gcgttcgccc gagagatcgc
      241 aacggagctt ctccggcgcg acgtcaagat gctcgtggtc gcgtgtaacg cgatcgaggt
      301 ggcggcgatc gaggacctca cggcagaggc cggtgttccc gtcgtcggtg tcatcgaggc
      361 cggcacacgg gcggcgctgc gcgcgacgag gaacgggcgc gtcggtgtca tcggcacgcg
      421 ggcgacgatc gagagcggcg cgtacgaacg cgcggtcgct cgcgaacgcg acggcgtcga
      481 gctcttctcg caagcctgtc cggcgttcgt gccgtacgtc gagcgcggcg acacgacgag
      541 cgaggatctg ctcgacatcg cgcacggata cctcgcgccg ctcgtcgccg cgagcatcga
      601 cacgctcgtg ctcgggtgca cgcattaccc gctgatctca gggctgctcc aggtcgtgat
      661 ggggccagat gtcctgctga tctcgtccgc cgaggagacg gcgaaggacg tctacgcgtc
      721 gctcgtgcgc gaggaccggc tccgcgacga cgacgttccg ccggcgcacg agttcctgtg
      781 ctccggcgac cccgggtcgt tcctcgcgct cggtggacgc ttcctcggac cggaggtcgt
      841 cgaggtccgg gcggcccgcg tcgatgcgag cccggttccg gagcgcccgt gatcctcacg
      901 gtcctcggtg cacatgggac gtggccgctc gccggcggcg cgacgagcgg atacctcgtc
      961 cggcacgacg gcttctcgct ctggatcgac tgcggatcgg gcacgatggc gaaccttcag
     1021 aaacacgtcg ggatgttcga cgtcgacgcg gtcgcgatct cgcacgtcca tcccgaccac
     1081 ctcgtcgacc tctacgcgta cttcttcgcc cgcgtctaca gccccgagaa gcccccgaag
     1141 cggccgctgt tcacgccgcc ggggctcctc gaccagttcg tcgtgatgct gcaggacacc
     1201 gcgatggagg agatgggcgg cacgttcgac ctcgtgacgg tcgagccggg ggagacattc
     1261 gaggccggtc ccttccgggt cgacacgcga ccgatgacgc acccggtccc gacgctcggc
     1321 atgcggatcg ccgccgacgg tgccgtgctc gcgtacacgg ccgacaccgg gccgaccgac
     1381 gagatcgtgc cgctggcgtc gggcgcggac ctgctcctcg cggaggcgac gtggcaggac
     1441 gaccatgagg aggcggcgcc gattcacctc accgcgcggc aggccggtga gatcggcgcg
     1501 cgcgtcgagg cgcggcgcct catgctcacg cacctgcgcc cccacctcga ccgggacaaa
     1561 tcccaggacg acgcgtcgtc gtcgttcccc ggcgaggtct cgctcgcagc ggaggacttc
     1621 tcgatcgagg tggggcgatg acgcgcacgg acggccgcgc gccggacgag ctccgaccga
     1681 tccgctacga cgtcggctac ctcgactggg cggagggatc ggtgctgctc gaggtcggac
     1741 ggacgcgggt cctctgctcg gcgtcgtggg agccacgggt gccgggcttc ctccacggca
     1801 gccgcaaggg atgggtgagc gccgagtact cgatgctgcc gcgcgcgacg caggagcgga
     1861 cgccgcggga ggcgagccgc ggacgcccgt ccggcaggac gcaggagatc cagcgcctca
     1921 tcggccgggc gctgcgcggg atcgtccggc tcgagaagct cggcgagaac acgatctggg
     1981 tggactgcga cgtgctccag gccgacggcg ggacgcggac cgcctcgatc accgggggat
     2041 acatcgcgct cgcgctcgcg ctgcgctcgc tgaaggagcg cggcgtcgtg cgagaggacg
     2101 tcctggtcga ctcggtcgcg gccgtgagcg ccggcatcgt cggcgacgag cgcctcctcg
     2161 atctcacgta cgaggaagac tcgcgcgccg aggtcgacct caacgtggtg atgaccggct
     2221 cccggcggct cgtcgagatc cagggcaccg ccgaggggca gccgttctcg cgcgacgacc
     2281 tcgacgcgct gatcgcgttc tcggagcgcg gcatcgatcg gctcacggcc gttcagcgcg
     2341 aggcggccgg cgcctgagcc gtggcgttcc cggagcggct cgtcatcgcc tcccggaacc
     2401 cggggaagat ccgcgagatc aggtcgatct gcgcggactg gccggtggcc tggctcacgc
     2461 acggggagct cgagtggccg gaggtcgagg agacagggga gtcgtacctc gaaaacgccc
     2521 tgctgaaggc ccgcagcgtc gccggtgcga ccggcgaggc ggcgctcgcc gacgactcgg
     2581 ggatcgaggt cgacgcgctc ggcggtgcgc cgggcccgcg gtcggcgcgg tacgccggcg
     2641 aggaggcgag cgacgaggcg aacctccgag cgctgatccg cgccgtcgcc ggggttccgg
     2701 cggggggccg gacggccagg taccgggccg tggcggtcgt ggcgtggccg ggcgggcgag
     2761 agctcaccgg cgagggcgcg tgcgagggtg tgctcgggac aaaaccgcga gggagcggcg
     2821 ggttcggcta cgaccccatc ttcgagccga tgggcgcagg tcgcacgatg gccgagctct
     2881 cgcccgagga gaaggaccgg atcagccacc ggggccgggc gctccgggcg ctccgcaccc
     2941 aactggaggg ttcggaagct cccgggtagc cgaccgggtg gaagatttcg ccgttctcag
     3001 gcgttatcct cactgagcag gccctggcgg cgcatgcccg gcctcgactc ccggatcgaa
     3061 gcgggcgtcg gcgcgcgcgc cgcgaggacc caacgaaacg gacatagcgt ccgcgtcagc
     3121 gggtatgcat ttgagaacgg ctagatggag aatgaagatg gcagcagtgc cccaggagat
     3181 caccgagaag gacgccaagc gggtcgaggc gttccagccg accgacgtcg acgacctccg
     3241 gctctacctc gaggccgccg ggcgtgagcc cctcctctcc aagcaggagg aggtcgagct
     3301 cgccatggcg atcgagcacg gcaaggaggc cgaggagaag ctgcggcgcg gccggatcaa
     3361 gtccgatcgc acgaagatgc ggctccgcga ggacatccgt aaggccgaac gcgcccggcg
     3421 ccggttcatc ctcgcgaacc tgcgcctcgt cgtgtcgatc gcgaagaagt acatcgggcg
     3481 cggcatgagc ttcctggacc tgatccagga ggggaacatc ggcctcatcc gggcggtcga
     3541 aaagttcgac tacgagaagg gcttcaagtt cagcacctac gccacctggt ggatccggca
     3601 ggccatcacc cgcgccatcg ccgaccacag ccggacgatc cgcatcccgg tccacatggt
     3661 ggaaacgatc aaccgcctca tgaa
//
LOCUS       DASZOB010000492         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_138528, whole genome shotgun sequence.
ACCESSION   DASZOB010000492 DASZOB010000000
VERSION     DASZOB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138528"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VG709_07690"
     CDS             complement(<1..979)
                     /locus_tag="VG709_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC32994.1"
                     /translation="MDVSLREVTKRFDDVVAVDDVSLEIGQGSFFALLGPSGCGKTTT
                     LRMIGGFEEPTSGTILLGDRDVTGLPPYRRDVNTVFQSYALFPHLSIFENVAFGLRRK
                     GVRGDSLRDRVGEALELVALSGLEKRKPRQLSGGQQQRVALARALVNRPRVLLLDEPL
                     GALDMKLRKQMQLELKRIQHEVGITFVHVTHDQEEAMTMADRIAVMHDGRIEQLGTPH
                     ELYERPETAFVAGFLGVSNLLQGSAGSDGVVRLDAGGDIVVRAGARDGRRGRGAVGIR
                     PEKVRLGPGEQNVLEGTIAERSYIGVSTQYIVETPAGSITVYSQNVQPGA"
     gene            complement(1042..>1599)
                     /locus_tag="VG709_07695"
     CDS             complement(1042..>1599)
                     /locus_tag="VG709_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWC32995.1"
                     /translation="VYDNLIDELVSAVESLHVGDPAEGEDVEMGPVISKVQQERVFGF
                     LERATQGGKARVLTGGDSNGERGFFVKPTVVADVDQDDEIVKREVFGPVVTVQRFADD
                     DQAIAWANDVDYGLSASVWTRDIGRALNAARKLEFGTVWINDHIPLTSEMPHGGFKQS
                     GYGKDLSVYSLEDYTVVKHVMAKID"
BASE COUNT          238 a          554 c          543 g          264 t
ORIGIN      
        1 gcgcgccggg ttggacgttc tgcgagtaga cggtgatcga gcctgcgggc gtctcgacga
       61 tgtactgcgt cgacacgccg atgtagcttc gttcggcgat tgtgccttcg aggacgttct
      121 gctcacccgg tccgagccgg accttctcgg gcctgatccc gacggcgccg cgcccgcgcc
      181 gtccgtcgcg cgcccctgcg cggacgacga tgtcgccgcc cgcgtcgagg cggacgacgc
      241 catcacttcc ggccgagccc tgaagcaggt tcgacacgcc gaggaagccg gcgacgaacg
      301 cggtctccgg acgctcgtag agctcgtgcg gcgtcccgag ctgctcgatc cggccgtcgt
      361 gcatgacggc gatccggtcg gccatcgtca tcgcctcttc ctggtcatgc gtcacgtgga
      421 cgaacgtgat cccgacctcg tgctggatcc gcttcagctc gagctgcatc tgtttgcgga
      481 gcttcatgtc gagcgcgccg agcggctcgt cgaggaggag gacgcgcggg cggttgacga
      541 gcgcgcgcgc gagcgcgacc cgctgctgct gaccaccgga gagctgacgc ggcttccgct
      601 tctcgagccc agagagggcg acgagctcga gcgcctctcc gacacggtcc cgaagcgagt
      661 cgccgcggac gcccttccgc cgcagcccga aggcgacgtt ctcgaagatc gagaggtgag
      721 ggaagagcgc gtagctctgg aagaccgtgt tcacatcgcg cctgtagggc ggaaggcccg
      781 tgacgtcgcg gtcgccgagc aggatcgtgc cggaggtcgg ctcctcgaag ccgccgatca
      841 tccgcagcgt cgtcgtctta ccgcaccccg aggggccgag gagcgcgaag aagcttccct
      901 gcccgatctc gagcgagacg tcgtcgaccg cgacgacgtc gtcgaagcgc ttcgtgacct
      961 cgcgcaggct gacgtccacg ggagatgccg gtgcctctgc gggggtcggg atccggtgct
     1021 cgcgtaccgc cacggcggag ctcagtcgat cttcgccatg acgtgcttca cgaccgtgta
     1081 gtcctcgagc gagtagacgg acaagtcctt gccgtacccc gactgcttga agccgccgtg
     1141 cggcatctcg gacgtcagcg ggatgtggtc gttgatccag accgttccga actcgagctt
     1201 gcgcgccgcg ttcagcgccc gcccgatgtc gcgtgtccag accgacgcgg agagcccgta
     1261 gtcgacgtcg ttcgcccagg cgatcgcctg gtcgtcgtcg gcgaagcgct gcaccgtgac
     1321 gacggggccg aagacctcgc gcttcacgat ctcgtcgtcc tggtcgacgt ccgcgacgac
     1381 cgtaggcttc acgaagaagc cgcgctcgcc gttcgagtcg ccgcccgtga gcacgcgggc
     1441 cttgccgccc tgcgtggcgc gctcgaggaa gccgaagacg cgctcctgct gaaccttcga
     1501 gatcacgggc cccatctcga cgtcctcgcc ctcggccgga tcgccgacgt gcagcgactc
     1561 gaccgccgag acgagctcgt cgatcaggtt gtcgtacac
//
LOCUS       DASZOB010000493         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_36729, whole genome shotgun sequence.
ACCESSION   DASZOB010000493 DASZOB010000000
VERSION     DASZOB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36729"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VG709_07700"
     CDS             complement(<1..422)
                     /locus_tag="VG709_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32996.1"
                     /translation="MAGRRTNLALLTLLAAAGATGALAYAIGSSWVRWVVVAHATAGI
                     AIVLLAPWKSVIARRGLRRGRSGSRASGAFTVLVAVAIVFGVLHATGLVVSIGGVTSM
                     QIHVGSALVSIPFAVWHVFARRVRVSRTDLSRRTLLRSG"
     gene            459..698
                     /locus_tag="VG709_07705"
     CDS             459..698
                     /locus_tag="VG709_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32997.1"
                     /translation="MAEQPSGPGDVWNLIRRADDLVKYSPNRDPRKARVQARDQLRRA
                     AQAAAMLPDRDAGEALAHQVETRLKDLDRLDREEA"
     gene            698..1270
                     /locus_tag="VG709_07710"
     CDS             698..1270
                     /locus_tag="VG709_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWC32998.1"
                     /translation="MASRIPPGQHLTRGWPVLHAEGIPRFDPETWRFRAYGDIEHETE
                     WSWEEFRSLPSVTVTSDFHCVTGWSKLDNEWEGVAFQAVAERVRPLPSVKHCVVSAPS
                     GYTANLPLDVLMDDDVLFAWSHGGEPLTKEHGGPMRLVVPKRYGWKSVKWANGVRFLE
                     RDSRGYWEERGYHNNAEPWAEERYSWQERR"
     gene            1462..2178
                     /locus_tag="VG709_07715"
     CDS             1462..2178
                     /locus_tag="VG709_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC32999.1"
                     /translation="MLRCILPLGDGTLVGTSAARLLRLRDRDLEPVAAFDRVPGRDAW
                     HTPWGGPPDTRSLARDAEGTVYVNVHVGGIVRSREGRWEPTIDIDTDVHHVVAHPERA
                     GALFAACAYGLATSTDGGDSWRIDDEGLHGSYCRAVAVAGDTMVVTASTGPFTRRAAV
                     YRAAVGGGSFERCTEGLPEWFTSNIDTYCLAAAEEVVAIATDDGAVYRSDDEGATWRQ
                     VAERLPAIHAIVVERTTTSA"
     gene            2106..>3061
                     /locus_tag="VG709_07720"
     CDS             2106..>3061
                     /locus_tag="VG709_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWC33000.1"
                     /translation="MAAGRGAVAGDPRDRRRADDDVRLTAFHSLRFRDYRLLWAGAVI
                     SNTGTWMQNVALSWYVFTLTRSAFWVGAVAFASFFPMWLSPLGGVLADRFDRRRILLA
                     TQSVMMIAATALAVLVGTGRATLAPVMGLTLLGGFAFSIDAPTRQAFFPSLVPRDAMV
                     NAIALNSAQFSLARVIGPAIAGTMIAAFGVAPVFWINAVSFLAVLAALTTVRERPRTM
                     EGEAPAQGLRAGLAYAWRHPVIRVLIASIAVLSVFAGPISALLPVFADEVFRRGPTGL
                     GALAAAMGAGSVLGAIVLGRIGRVRPRMVAAGVALAGGALTL"
BASE COUNT          425 a         1093 c         1113 g          430 t
ORIGIN      
        1 cccgaacgca gcagggtccg ccgcgagagg tccgtccgcg agacccgcac gcggcgggcg
       61 aagacgtgcc agacggcgaa cgggatcgag accagcgcgg acccgacgtg gatctgcatc
      121 gacgtcacgc cgccgatcga gacgacgagc ccggtggcat ggaggacgcc gaagacgatc
      181 gccacggcga cgagcacggt gaaggcgccc gacgcgcgcg accccgatcg tccacgccgg
      241 agcccgcgac gcgcgatcac ggatttccag ggcgccagca gcacgatcgc gatgccggcg
      301 gtcgcgtgcg ccacgacgac ccagcgcacc caagacgacc cgatcgcgta cgcgagcgca
      361 cccgtcgccc ccgcggcggc caggagggtg agcagcgcga ggttggtccg ccgccccgcc
      421 acgccgcgcg cctactccgg agatatgctt cgcccgaggt ggcggagcaa ccgagcggcc
      481 ccggcgacgt gtggaacctg atccgccgtg ccgacgacct cgtgaagtac tccccgaacc
      541 gagatccgcg gaaggcccgg gtccaggccc gggaccagct ccggcgagcc gcacaggcgg
      601 ccgcgatgct ccccgaccgc gacgcgggcg aggcgctcgc gcaccaggtc gagacgcggc
      661 tcaaggacct cgaccggctc gaccgcgagg aggcctgatg gcgtcccgaa tccctcccgg
      721 ccagcacctc acacgcggat ggccggtcct ccacgccgag gggatcccgc ggttcgaccc
      781 cgaaacgtgg cgcttccgcg cgtacggcga catcgagcac gagacggagt ggtcctggga
      841 ggagttccgg tcgctcccct cggtcacggt cacgtcggac ttccactgcg tcaccgggtg
      901 gtcgaagctc gacaacgagt gggagggagt tgcgttccaa gccgtcgcgg agcgagtacg
      961 tccgctgccg tcggtgaagc actgcgtggt atcggcacca tccggctaca cggcgaacct
     1021 cccgctggac gtgctgatgg acgacgacgt gctgttcgcc tggtcgcacg gcggcgagcc
     1081 gctcacgaag gagcacggcg ggccgatgcg cctcgtcgtc cccaagcgct acgggtggaa
     1141 gagcgtgaag tgggcgaacg gcgtgcgctt cctcgagcgt gactcgcgcg ggtactggga
     1201 ggagcgcggc taccacaaca acgccgagcc ctgggccgag gaacgctact cctggcagga
     1261 gcgtcgctga cccgcatcct cgccgccacg aacgacggca tcgtcgggat cggctcgaac
     1321 gagcaggcgc tcgaacgcca gccggtcacc gccctcgcgc tcgccgacga cggcgtctgg
     1381 gcgatcgtct ccgagctcga cgtctggcgc tcggcggacg gcggttggcg cagagctgcg
     1441 ggcgcgcgag acatcggggt cgtgctccga tgcatcctcc cgctcggcga tggcacgctg
     1501 gtcggcacgt cggcggcgcg cctccttcgg ctgcgggaca gggatctcga gccggtcgcg
     1561 gcgttcgacc gcgtgccggg aagagacgcc tggcacacgc cgtggggcgg gcctccggat
     1621 acccgttcgc tcgcgcgcga cgcagagggg accgtgtacg tcaacgtcca cgtgggcggc
     1681 atcgtccgct cgcgcgaggg gcggtgggag ccgacgatcg acatcgacac cgacgtccac
     1741 cacgtcgtcg cgcatcccga gcgcgccggc gcgctgttcg cggcgtgcgc gtacgggctc
     1801 gcgaccagca ccgacggcgg cgactcgtgg cgcatcgacg acgaaggcct ccacggcagt
     1861 tactgccgtg cggtcgcggt ggcgggcgat acgatggtgg tgaccgcctc caccgggccg
     1921 ttcacgcggc gggcggccgt gtaccgggcg gccgtcggtg gcggctcgtt cgagcgctgc
     1981 acggaagggc ttcccgagtg gttcacgtcc aacatcgaca cctactgcct cgccgccgca
     2041 gaggaggtcg tcgcgatcgc gacggacgac ggcgccgtct atcgctccga cgacgagggg
     2101 gcgacgtggc ggcaggtcgc ggagcggttg ccggcgatcc acgcgatcgt cgtcgagcgg
     2161 acgacgacgt ccgcctgacc gcgttccact cgctgcgctt ccgggactac aggctgctgt
     2221 gggccggcgc cgtcatctcg aacacgggga cctggatgca gaacgtcgcg ctctcctggt
     2281 acgtgttcac gctgacgcgt tcggcgttct gggtgggcgc ggtggccttc gcgagcttct
     2341 tcccgatgtg gctctcgccg ctcggcggcg tgcttgccga ccgcttcgac cgccgaagga
     2401 tcctgctcgc gacgcaatcg gtgatgatga tcgccgcgac ggccctcgcc gtgctcgtcg
     2461 ggacggggcg ggcgacgctg gcgccggtca tgggcctcac gctcctcggc ggcttcgcct
     2521 tctcgatcga cgccccgacg cggcaggcgt tcttcccgag cctcgtgccg cgggacgcaa
     2581 tggtgaacgc gatcgcgctg aactccgccc agttcagtct cgcgcgggtg atcggccccg
     2641 cgatcgccgg cacgatgatc gcggcgttcg gcgtcgcgcc cgtgttctgg atcaacgccg
     2701 tttcgttcct cgctgtcctc gcggcgctca cgaccgtgcg ggaacggcca cgaacgatgg
     2761 agggtgaggc gccggcgcag gggctccgcg cggggctcgc ctacgcgtgg cgccatcccg
     2821 tgatccgggt ccttatcgcg tcgatcgcgg tcctctccgt gttcgccggg ccgatctccg
     2881 cgctcttgcc ggtcttcgcc gacgaggtct tccgccgcgg ccccaccggg ctcggggccc
     2941 tcgccgccgc catgggggcg ggatcggtgc tcggcgcgat cgtcctcgga aggatcggcc
     3001 gcgtgcggcc gcgcatggtc gcggccggcg tggcgctcgc cggcggcgca ctgacgctgt
     3061 t
//
LOCUS       DASZOB010000494         4674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_16694, whole genome shotgun sequence.
ACCESSION   DASZOB010000494 DASZOB010000000
VERSION     DASZOB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16694"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VG709_07725"
     CDS             complement(<1..827)
                     /locus_tag="VG709_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWC33001.1"
                     /translation="MIVRLAAVFAHPDDDAWTIGGTLAMHARELDPTIVLCTSGGNGP
                     IWEPVATRETLAQVRESEQREWCAAIGIPNARVEFFRYTDGDLATVGREELIGRLTSI
                     LEEVQPHVVVTVGPDGMTRHADHIRAGEAGGEAFHRARSASDGGSGGFARLYHVALRR
                     STLPEYYRAARERHLPLGDDETPLNPVGGPDDRVAGDVDVSRVYDVKVEAIARHRSQI
                     GELLRIPRDLQPLHLAHERSGQAWPTRDGDAMVAADLFSGVEKRVTDATVVVVGSIN"
     gene            complement(838..1521)
                     /gene="hxpB"
                     /locus_tag="VG709_07730"
     CDS             complement(838..1521)
                     /gene="hxpB"
                     /locus_tag="VG709_07730"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HWC33002.1"
                     /translation="MEAQAIEAVIFDMDGVLVDSEPMWRAVEREVFSRLGIDVSEENL
                     VETLGVPIDEVVALWHRRHPGGEPTRDEVVQTIVAGVADGIAREGLNPGAIKAVDYFR
                     ERGLRTALASSSPMSLIRAVLSVDGLGERFEVVHSAEHEERGKPEPDVYVSTARAMGV
                     DRERCLAIEDSLNGVRSAKAAGMVCIALPETPPDDGPFGDADLVIRSLAELDDRIWSA
                     TDTVPAPRA"
     gene            complement(1584..2201)
                     /locus_tag="VG709_07735"
     CDS             complement(1584..2201)
                     /locus_tag="VG709_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWC33003.1"
                     /translation="MTGDYVPFIDDPEHLREVDGERYAVLRPTDAVVAAYSDVRTRLR
                     EVLAGSNASFPAYPHVTLRGWPKETDLDRVRDVVSRWSRRIPPLDLQIERVGTFPSPY
                     RIVVVIVQKTPALRDAFTSLVAASADAGLPIWPAWTGDVDAWEFHMSVAYCSALDEEA
                     WMRVAASAVAFEIPRAACEVREIEVAAFDDGRERSGGSFLLDGSR"
     gene            complement(2198..4348)
                     /locus_tag="VG709_07740"
     CDS             complement(2198..4348)
                     /locus_tag="VG709_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33004.1"
                     /translation="MSLPQRTSDAPPRIERGVYFDAWFPLQHNYHPSFPPRRLRMIDH
                     LEDYRATMLVWAALGGGNLSLPYLEEEAYAEVPPRYRVYGFVNDREFIEECGRRGIKV
                     FGVVFEHAWEYPVELSDDGSEVLALNELRGVGKRDWLGMREFWQNRYPNLWPSREKYF
                     PEPVLNADGEVVEDILEECAQRDIHGNALHALWIECPDREHWNYMMDRNNPVWREYLK
                     AIVRLQIDAGVQGVHFDEAEVPLTSLQYGGCFCKTCMRGFREHLEVMPEGERPEELRG
                     EDLSTFHYGEWLLARGYDFKPPQGTTPLFQSYVAFQQRNVARYFKELADYTREYAASV
                     GREVKVSGNFFNLFESYYPIEPHVDVVVTEMRNTLWRQPEWYRHAAGFARGKPLVVAE
                     NPYGGVVMELVEGLKRGRMYDRFRQSLYEAAAMGVNMSVPYGAWMGSVVEDAFYAPHE
                     LAVEIQTFIADHEHLYGRDPSLAEVGVIFEVTSNSRVRAAAELPADNRFNVLPESDIL
                     AFDQVSRVLAAAAQPYDAVFFPDGDLRPDTLTETDLLRYRTLIAPACEELTERQLELL
                     ERYVEAGGRLVVLGEPGTNLGERVRRVLDHPAVVSGDPFRFSLDLLPEGPQVEVVDGT
                     TDVALTLQEVEQGCALHLIRYDYDEKQDRVPPLDRLELSLRLPFAVGSVEAFSPDGRL
                     SAAGTRRDDGRHELVLRDVPVYGLVLLRLDPTGR"
     gene            complement(4345..>4674)
                     /locus_tag="VG709_07745"
     CDS             complement(4345..>4674)
                     /locus_tag="VG709_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC33005.1"
                     /translation="DDASLATGAPDGRRMKTRVELLEDMGHEAFVHFQIAAPPVVTED
                     TKELAADAGADVSELEQAARKQRETTFIARLDPHTKARETESFEIVVDTRQLHFFDQE
                     TGLAVWE"
BASE COUNT          721 a         1686 c         1556 g          711 t
ORIGIN      
        1 atgttgatgc ttccgaccac gaccaccgtc gcgtccgtca cccgcttctc gacgcccgag
       61 aagaggtccg cggcgaccat cgcgtcgccg tcgcgagtgg gccacgcctg gccggagcgc
      121 tcgtgcgcca agtggagcgg ctggaggtcc cgagggatcc gcagcagctc gccgatctgc
      181 gaccggtgtc gcgcgatcgc ttcgaccttt acgtcgtaga cgcggctcac gtcgacgtcg
      241 cccgcgacgc ggtcgtccgg gccgccgacg ggattcagcg gcgtctcgtc gtcgccgagc
      301 gggaggtgcc gctcacgggc ggcgcggtag tactccggga gcgtcgagcg acgcagcgcg
      361 acgtggtaca gccgcgcgaa cccgccggac cctccgtccg acgcggagcg cgcacgatgg
      421 aacgcctcgc cgccagcttc tcccgcgcgg atgtgatccg cgtgccgcgt catcccgtcg
      481 ggcccgaccg tgacgacgac gtggggctgc acctcctcga ggatcgaggt gaggcggccg
      541 atgagctcct cgcggcccac ggtggcgagg tccccgtccg tataccgaaa gaactcgaca
      601 cgggcgttcg ggatcccgat cgccgcgcac cattcgcgct gctcactctc gcgcacctgc
      661 gccagcgtct cgcgggtcgc gaccggctcc cagatcgggc cgttgccgcc gctcgtgcac
      721 agcacgatcg tcgggtcgag ctcccgggcg tgcatcgcga gcgtcccgcc gatcgtccac
      781 gcgtcgtcgt cggggtgcgc gaacacggcg gccagccgca cgatcacgcg gctcccgtca
      841 ggcgcgaggc gccgggaccg tgtcggtcgc cgaccagatg cgatcgtcga gctcggcgag
      901 cgaccggatc acgaggtccg cgtcgccgaa gggtccgtcg tcgggcggcg tctcgggcag
      961 cgcgatgcac accatgcccg cggccttcgc cgaccgcacc ccgttgaggg agtcctcgat
     1021 tgccaggcac cgttcccgat cgacgcccat cgctcgcgcc gtcgagacgt agacgtccgg
     1081 ctccggcttc ccccgctcct cgtgctccgc ggagtgcacg acctcgaacc gctccccgag
     1141 cccatcgacg gagagcaccg cacggatcag gctcatcggc gaggatgacg cgagcgccgt
     1201 acgcagccca cgttcgcgga agtagtcgac ggccttgatc gcgccggggt tcaggccctc
     1261 gcgagcgatc ccgtcggcga ctccagcgac gatcgtctgg acgacctcgt cgcgcgtcgg
     1321 ttcgcccccc gggtgccgcc gatgccacag cgccacgacc tcgtcgatcg ggacgcccaa
     1381 cgtctcgacg aggttctcct cgctgacgtc gatcccgagc cgcgagaaca cctcgcgctc
     1441 gacggcgcgc cacatcggct cggagtcgac aagcacgccg tccatgtcga agatcaccgc
     1501 ctcgatcgcc tgagcctcca tggcacccga tgatacggag ccccgcgagg tcgacgcagt
     1561 ccggcgtccg atgtctcatc cgctcaacgg gagccgtcga ggaggaagct cccgccggat
     1621 cgctcgcggc cgtcgtcgaa cgccgccacc tcgatctccc gaacctcgca cgccgcccgc
     1681 gggatctcga acgccacggc ggacgccgca acccgcatcc atgcctcctc gtcgagagcg
     1741 ctgcagtacg cgaccgacat gtggaactcc cacgcgtcga cgtcgccggt ccatgcgggc
     1801 catatgggaa gccccgcgtc cgccgacgcc gcgacgagcg aggtgaaggc gtcccggaga
     1861 gccggggtct tctggacgat cacgacgacg atccggtagg gggatgggaa cgttccgacg
     1921 cgctcgatct gcaggtccag cggcgggatc cgcctcgacc accgcgacac gacgtcgcgc
     1981 acacggtcga ggtcagtttc cttcggccac cctcggagcg tgacgtgcgg gtacgccggg
     2041 aacgaagcgt tcgagcccgc cagtacctcg cgtagtcggg tccggacgtc ggaataggcc
     2101 gcgacgaccg cgtcggtcgg tctaaggacg gcgtatcgct ctccatcgac ctcgcgaagg
     2161 tgctcgggat cgtcgatgaa cgggacgtag tcacccgtca acgacccgtc gggtccagcc
     2221 tcagcagcac gagcccgtag acagggacgt cgcggaggac gagctcgtgg cggccgtcgt
     2281 cacggcgcgt cccggcggca gaaagccgcc cgtccgggct gaacgcctcg acacttccga
     2341 ccgcgaacgg cagccggagc gacagttcga gccggtcgag cggcggcacg cgatcctgct
     2401 tctcgtcgta gtcgtaccgg atgagatgaa gcgcgcatcc ctgctccacc tcctggagcg
     2461 tcagcgcgac gtcggtggtt ccgtcgacca cctcgacctg tggtccctcc ggaagcaggt
     2521 cgagcgaaaa gcgaaacggg tcgccgctca cgacggcggg gtggtccagg acgcggcgca
     2581 cccgctcgcc gaggttcgtc ccgggctccc cgaggaccac gagacgcccg ccggcctcga
     2641 cgtagcgctc gagcagctcg agctgccgct cggtgagctc ctcgcacgcc ggcgcgatca
     2701 gcgtccggta ccggaggagg tccgtctccg tgagcgtgtc ggggcgcaga tccccgtcgg
     2761 ggaagaagac ggcgtcgtac ggctgggccg cggcggcgag cacccgcgag acctggtcga
     2821 acgccaagat gtcgctctcc ggaaggacgt tgaaccggtt gtccgcgggg agctcggccg
     2881 ctgcccgtac ccgcgagttg ctcgtgacct cgaagatcac gccgacctcg gccaaggacg
     2941 ggtcgcgccc atacaggtgc tcgtggtcgg cgatgaacgt ctgaatctcg acggcaagct
     3001 cgtgcggagc gtagaacgcg tcctcgacga cgcttcccat ccacgcgccg tacgggacgc
     3061 tcatgttcac gcccatcgcg gccgcctcgt agagcgactg ccggaaacgg tcgtacatcc
     3121 ggccgcgctt caacccctcg accagctcca tgaccacgcc gccgtacgga ttctccgcca
     3181 cgacgagcgg cttcccgcgc gcgaaccccg ctgcgtgccg gtaccactcc ggctgccgcc
     3241 agagcgtgtt gcgcatctcg gtgaccacga cgtcgacgtg cggctcgatc gggtagtagg
     3301 actcgaacag attgaagaag ttcccggaga cctttacctc gcggccgacg ctggcggcgt
     3361 actcgcgcgt gtagtccgcg agctccttga agtaacgtgc gacgttgcgc tgctggaagg
     3421 ccacgtagct ctggaacagg ggcgtcgtcc cctgcggcgg cttgaagtcg tagccgcgcg
     3481 cgagcagcca ctcgccgtag tggaacgtcg agaggtcctc cccgcgcagc tcctcgggtc
     3541 gctctccttc gggcatcacc tcgaggtgct cgcggaaccc gcgcatgcac gtcttgcaga
     3601 agcagccgcc gtactggagc gacgtgagcg gcacctccgc ctcgtcgaag tgcactccct
     3661 ggacgccggc gtcgatctgg aggcgcacga tcgccttgag gtactcgcgc cacaccgggt
     3721 tgttgcgatc catcatgtag ttccagtgct cgcggtccgg gcactcgatc cacaacgcat
     3781 ggagcgcgtt cccgtggatg tcgcgctgcg cgcactcctc gaggatgtcc tcgacgacct
     3841 cgccgtcggc gttcagcacc ggctcgggga aatacttctc gcgcgaaggc cagagattcg
     3901 ggtagcggtt ctgccagaac tcgcgcatgc cgagccagtc gcgcttcccg acgccgcgga
     3961 gctcgttgag cgcgaggacc tccgacccgt cgtcggagag ctcgaccggg tactcccagg
     4021 cgtgctcgaa gacgactccg aacaccttga tcccgcgccg cccacactcc tctatgaact
     4081 cgcggtcgtt gacgaacccg tagacccggt accgcggcgg tacctcggcg tacgcctcct
     4141 cctcgaggta cggaagcgag aggttgccgc cgccgagcgc cgcccacacg agcatcgtcg
     4201 cgcgatagtc ctcgaggtga tcgatcatcc gcagccgccg cggggggaaa gacggatggt
     4261 agttgtgctg cagcgggaac cacgcgtcga agtagacgcc ccgctcgatc cggggcggag
     4321 cgtcggaggt gcgctgcggc aggctcactc ccagacggcc agtccggtct cttggtcgaa
     4381 gaagtgcagc tgcctggtat ccacgacgat ctcgaacgac tccgtctcgc gcgccttcgt
     4441 gtgcgggtcc aggcgcgcga tgaacgtcgt ctcgcgctgc ttcctcgccg cctgctccag
     4501 ctcggagacg tcggcaccgg cgtccgcggc gagctccttc gtgtcctcgg tcacgaccgg
     4561 aggggcggcg atctggaagt ggacgaaggc ctcgtgcccc atgtcctcga ggagctcgac
     4621 ccgcgtcttc atccggcgac cgtccggcgc cccggttgcg agactcgcgt cgtc
//
LOCUS       DASZOB010000495         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_16835, whole genome shotgun sequence.
ACCESSION   DASZOB010000495 DASZOB010000000
VERSION     DASZOB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16835"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1007)
                     /locus_tag="VG709_07750"
     CDS             complement(144..1007)
                     /locus_tag="VG709_07750"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWC33006.1"
                     /translation="MTTVAFVGLGAMGSRMAARLLDAGHDLVAWNRTREKADALAERG
                     AAIAETPADAAARAEVVITMVADPDALRAVTEGPEGVLLSAGDATTVIEMSTVGPAAV
                     SRLRAALPEAAGLLDAPVLGSIDAAETGELEVFVGGSEELYDRWAPLLSALGSPIHVG
                     RLGSGAAAKLVANSTLFGVLGALGEALALARGLGLSEDATFGILSNTPLGSQAERRRS
                     AFESDDYPLRFSLSLARKDVDLMLAAAADGGVELRLAAAARSWLAEAEASGWADRDYA
                     SVLSWIARRPT"
     gene            complement(1035..>1757)
                     /locus_tag="VG709_07755"
     CDS             complement(1035..>1757)
                     /locus_tag="VG709_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWC33007.1"
                     /translation="VRRLSIPRGRALIGPVLYGVFLGGAFGAAYYALITIQAGLGQML
                     LALVPLATLLLAVAVGQERLRVASLVGSVVAIAGIVVISAGSFGGSVPVLPLLAVFGG
                     VLCFSAATVTARRFPSVHPVMMNAVGMTTAAVLLISGSAIAREPFELPDLLATWMAVA
                     YIAAVGSIVVFVLYLVVVRLWNASRAAYTFVITPVVTVLLSAWLDNEPIGLGLVLGGL
                     LVLSGVYVGALRPTTAAGDDTG"
BASE COUNT          290 a          645 c          628 g          194 t
ORIGIN      
        1 cgcccgcccg cggtgccgga tctgcgggtc cgacgacctc cgatacgccc ccgagccgct
       61 gtgggagcgc ggcaggggag accagcgaac gccggcgggc cggcgggacg cctacgactg
      121 ctacgcgtgt ggcggcaagg acgtcacgtc ggtcggcgcg cgatccagct gaggaccgac
      181 gcgtagtcgc ggtcggccca accgctcgcc tccgcctcgg ccagccagct gcgcgcggca
      241 gccgcgagac gcagctcgac gccgccgtcc gccgcggccg cgagcatgag gtcgacgtcc
      301 ttgcgggcga gcgatagcga gaaccggagc ggatagtcgt cgctctcgaa cgccgaccgt
      361 cgtctctcgg cctgcgaacc caacggggtg ttcgagagga tcccgaacgt cgcgtcttcc
      421 gaaagcccga ggccgcgcgc gagcgccagc gcctcgccga gcgcgccgag gaccccgaac
      481 agcgtcgagt tcgcgacgag cttggccgcc gctccggagc cgagccgccc gacgtggatc
      541 ggcgacccga gcgccgacag cagcggcgcc cagcggtcgt agagctcctc ggagccgccg
      601 acgaacacct cgagctctcc ggtctcggcc gcgtcgatgc tgccgagcac cggggcgtcg
      661 aggagccccg cggcctccgg tagcgccgcc cggaggcgcg agacggccgc ggggcccacc
      721 gtcgacatct cgatgacggt ggtcgcgtcg cccgccgaga gaaggacgcc ttcggggcct
      781 tcggtcacgg cccgaagcgc atccgggtcg gcgaccatcg tgatcacgac ctcggcgcgc
      841 gctgctgcgt ccgcaggcgt ctcggcgatg gcggcacctc gctccgcgag cgcgtcggcc
      901 ttctcgcgcg tgcggttcca tgccacaagg tcgtgaccgg cgtcgaggag ccgcgccgcc
      961 atgcggctgc ccatcgcacc cagcccgacg aaggcgaccg tcgtcatcgt cgccagtatg
     1021 cgcgacgccg agggtcagcc cgtgtcgtcg cccgccgcgg tcgtcggtcg aagcgcgccg
     1081 acgtagacgc cggacagcac gaggagccct ccgaggacca ggccgagccc gatcggttcg
     1141 ttgtcgagcc aggccgagag aagcaccgtc acgaccggcg tgatgacgaa cgtgtacgcg
     1201 gcgcgcgacg cgttccacag cctcacgacg acgaggtaga gcacgaagac gacgatcgag
     1261 ccgacggcgg cgatgtaagc gacggccatc cacgtcgcga ggagatccgg cagttcgaac
     1321 ggctcgcgcg cgatcgccga tccggagatc aggaggacgg cggcggtcgt catccccacc
     1381 gcgttcatca tgactgggtg gacggacggg aaccggcgcg ccgtcacggt cgcggccgag
     1441 aagcacagga ccccgccgaa gaccgcgagt agcgggagga ccggcacgga acccccgaac
     1501 gatccggccg agatgacgac gatgcccgcg atggcgacga cgctcccgac gagcgacgcg
     1561 acgcggaggc gttcctgccc caccgcgacc gcgagcagca gcgtcgcgag cggcaccagc
     1621 gccagcagca tctgtccgag acctgcttgg atcgtgatca gcgcgtagta agcggcaccg
     1681 aacgccccgc ccaggaagac cccgtagagc accggcccga tcagcgctcg accgcgcggg
     1741 atcgacagcc tgcggac
//
LOCUS       DASZOB010000496         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17036, whole genome shotgun sequence.
ACCESSION   DASZOB010000496 DASZOB010000000
VERSION     DASZOB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17036"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VG709_07760"
     CDS             complement(<1..209)
                     /locus_tag="VG709_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33008.1"
                     /translation="MSERAATTMETSGFLAQTGAFTPDGPVARDIADLWWLMLAVGTA
                     VYVLFLFLFAVGAFRSRTSREERGD"
     gene            complement(206..1267)
                     /locus_tag="VG709_07765"
     CDS             complement(206..1267)
                     /locus_tag="VG709_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HWC33009.1"
                     /translation="MRVLDLRGLPLWLQFAVFVLAAAVVWIAAVRASRYAGAISRKTR
                     LGQGFVGMVLLAVATSLPELGATATASVTGNAPLAANNLLGGVAFQTTVLAIADAASR
                     RRLTAEARGPSPLMQGAALVLVLSLVLAGISVGGGPALAGASVWTIALAVAYLVSAVI
                     VRRETASRLPAAGDTPGPRPEGSLSERSGEVSRYGDTAISALAFRFGVAATIILLAGS
                     ALARTAEAIADGTGLGAGFVGATLLALATSLPEVGSTVEAARQGLETMAIGNVLGSNA
                     MDVFLLFVADMLFSGGPILGRIGAGSAFLAAVAISLTAVYLFGLLLRRTGTVGRVGVD
                     SIVVAALYVTTVVVLYVIR"
     gene            1776..2480
                     /locus_tag="VG709_07770"
     CDS             1776..2480
                     /locus_tag="VG709_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33010.1"
                     /translation="METAQPQPRDTGTGERMADLARAKTSAITHKLQGEEQDQTGARF
                     VVEAAEAERLTEDWPAAPRKIARQMIERYGAPNEATPVRLVWHRNRPWKRTQVARDEI
                     VHNFPTSHTDFLTQWIDYSVPPRRFSDIAEFDGSCLADRTAGEAAARCDSEAMNILTL
                     NLMHEIVTGSRAVDEARQVYAENAAAFTMGQDAPYAERLLFDVPEGGAVDPDEKVIGE
                     AMAQKAKEAVTSDGSA"
     gene            complement(2490..3185)
                     /locus_tag="VG709_07775"
     CDS             complement(2490..3185)
                     /locus_tag="VG709_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWC33011.1"
                     /translation="MPTTLQDASWLVRSLRPEMSHPVGDEALAALAANLERKRLPAGQ
                     LLYRRGARPAGVWAIRSGTAELSIRGARTRSVVQLLRPGDVAGDVSVLLGSPALTTAR
                     VKGPGTFLFLRRDVARSIVSTHADLCFLWMQNVAFRLEGARRRILEVLGEDLSQAVSR
                     LLLDEEVGGSVRLAQSAIADMLGVQRSSVNRVLRSLHRRGIVTPSYGTVTINDRDALE
                     DIAVVTGGGFDAP"
BASE COUNT          600 a         1190 c         1287 g          462 t
ORIGIN      
        1 tcatcccccc gctcttcacg ggacgtcctg ctcctgaacg cgcccacggc gaacaagaag
       61 aggaacagga cgtagacggc ggtgccgacc gcgagcatca gccaccagag gtccgcgatg
      121 tcgcgcgcca ccggaccgtc gggcgtgaac gcgcccgtct gcgcgaggaa gcccgaggtc
      181 tccatcgtgg tggcggctcg ttcgctcatc gaatcacgta gagcacgacg acggtcgtta
      241 cgtagagcgc cgcgacgacg atagagtcga cgcccacgcg gccgaccgtt ccggttcgtc
      301 ggaggagcaa cccgaacagg tacacggctg tcagggagat cgccacggcg gcgaggaacg
      361 ccgagccggc accgatccga ccgaggatag ggccgcccga gaacaacatg tccgcgacga
      421 agaggaggaa gacgtccatg gcgttgctcc caaggacgtt cccaatcgcc atcgtctcaa
      481 gcccctggcg ggccgcttcg acggtgctcc cgacctccgg cagcgacgtc gccaacgcga
      541 ggagggtcgc gccgacgaac ccggcgccta gcccggtccc gtccgcgatc gcctcagccg
      601 tccgggcgag ggcgcttccg gcgagcagga tgatcgtggc cgcgacgccg aaccggaacg
      661 cgagcgccga aatcgccgta tcgccgtacc ggctcacttc accggaacgc tcggacagcg
      721 acccctcggg ccgcggtccg ggcgtgtcac cggcggcggg cagtctcgag gcggtctccc
      781 tccgcacgat cacggcggag acgaggtacg cgacggcaag cgcgatcgtc cagacgctcg
      841 cgccagcgag tgcggggcct cccccgaccg agatcccggc gaggaccagc gaaagcacga
      901 ggacgagcgc cgcgccctgc atgagcggcg aagggccgcg ggcctcggcc gtgaggcgcc
      961 gtcgcgacgc agcgtcggcg atcgcgagta cggtcgtctg gaacgcgacg cctccaagga
     1021 ggttgtttgc tgcgagcgga gcgtttccgg tgaccgacgc cgtcgccgtc gctccgagct
     1081 cgggtagcga cgtggcgacc gcgaggagaa ccattccgac gaacccctga ccgaggcgcg
     1141 tcttgcgcga gatcgcgccg gcgtaccgcg acgctcggac ggcggcaatc cacacgaccg
     1201 ccgcggcgag gacgaacacg gcgaactgca gccataacgg gagaccgcgc agatcgagga
     1261 cgcgcacgac cgcgtcagcc gcctacgggt ggtggtcacc cgttgccacg gatgccggct
     1321 taccccgcgc ttcgtgcgcg acacgccgct gcggttcgaa atgtcgggta ggcgacaaag
     1381 ttcatggaac ggatccaggg gcgcgacgat tcgccgtttc cgcggccgct ggtttcgcgc
     1441 gtcgttcgcg ccggcaacgg gacctaaagg tgaggcggtt gcaagagcaa agaggaggag
     1501 gtcgacgcga gcaatctgga aacggagcgc cggaatgtga gtggtcgcgt gccgacacgt
     1561 gggtgacgat ggcggcgttc gatcgcatcg cgctcgtcgg cctgttcgcg gtggccctac
     1621 ctcagcctac gagacgtctt cctcgtcctt cagcgcgccg ggtcgaggct gtccgccacg
     1681 tgacagtttc acggcggctt gtgttgcgcg cgggtatggg tcgcgtggag cacgacgacg
     1741 gccgagcgcg tttcgcgctt gaaggaggaa gcgacatgga gactgcccag ccgcaaccac
     1801 gcgatacggg caccggcgag cgcatggcgg acctcgcccg tgcgaagacg agcgcgatca
     1861 cgcacaagct gcagggggag gagcaggacc agaccggggc gcggttcgtc gtcgaggcgg
     1921 ccgaagcgga gcggctgacc gaggactggc cggcggcccc gcgcaagatc gcgcggcaga
     1981 tgatcgagcg gtacggcgcc ccgaacgagg cgacgcccgt gcgcctcgtg tggcatcgga
     2041 accgtccgtg gaagcggacg caggtggcgc gggacgagat cgtgcacaac ttcccgacct
     2101 cgcacaccga tttcctgacg cagtggatcg actacagcgt gccgccgcgc cgcttcagcg
     2161 acatcgcgga gttcgacggg agctgcctgg cggaccgcac ggcgggtgag gccgcggcgc
     2221 ggtgcgacag cgaggcgatg aacatcctca cgctgaacct catgcacgag atcgtcaccg
     2281 ggagccgcgc ggtggacgag gcgcgacagg tctacgccga gaacgccgcc gcgttcacga
     2341 tggggcagga cgctccgtac gccgagcgcc tgctgttcga cgtgccggag ggcggcgcgg
     2401 tcgatccgga cgagaaggtc atcggcgagg cgatggccca gaaggcgaag gaggccgtca
     2461 cgagcgacgg ctcggcatag gacgacgatc tagggcgcgt cgaacccgcc gccggtcacg
     2521 accgcgatgt cctcgagggc atcgcggtcg ttgatcgtga cggtgccgta cgacggcgtg
     2581 acgatcccgc ggcggtgcag cgaccgcagc acgcggttca cggagctccg ctggacgccg
     2641 agcatgtcgg cgatcgccga ctgcgcgagc cggaccgacc ccccgacctc ctcgtcgagg
     2701 agcaggcgcg ataccgcctg tgacagatct tcacctagga cctccaggat ccgtctgcgg
     2761 gcgccctcga gccggaacgc gacgttctgc atccatagga agcacagatc ggcgtgcgtc
     2821 gacacgatcg accgcgccac atccctccga aggaacagga acgtcccggg gcccttcacg
     2881 cgggccgtcg tcagcgccgg ggacccgagc aacaccgaca cgtcgccggc gacgtctccg
     2941 gggcggagga gttggacgac ggatcgcgtt cgagcgccgc ggatcgagag ctccgccgtc
     3001 cccgacctga tcgcccagac ccccgccggc cgcgcccctc gtcggtacag cagctggccg
     3061 gcggggaggc gcttccgctc gaggttcgcc gcgagcgccg cgagcgcctc gtctccgacg
     3121 gggtggctca tctccgggcg gaggctgcgg acgagccacg acgcgtcctg gagcgtggtc
     3181 ggcatcgtcc cgaatgtagc gccttgcgat cggtttgctg agccccaggc ctcagtaggc
     3241 tggctgcgtg cgcgacatct cgtgtccctc ctgcggcgaa gcggagcgtc ttcgcgggac
     3301 ctcggggccc gaccaagtgg tccgggtcga gtgcgaggcg tgtggggcga cgtgggagcg
     3361 ggatgctcgg ccacggtgcc ggctctgcgg atcggacgat gttcggtacg cgccgaagcc
     3421 gctctgggag cgagggagag ggcagcagcg cacgccggcc ggccggcgag acgcctacga
     3481 ctgctacgcg tgtggcggca cggacgtcac gtccgtcggg gcgcgatcca gttgaggat
//
LOCUS       DASZOB010000497         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_47687, whole genome shotgun sequence.
ACCESSION   DASZOB010000497 DASZOB010000000
VERSION     DASZOB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47687"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="dnaB"
                     /locus_tag="VG709_07780"
     CDS             <1..882
                     /gene="dnaB"
                     /locus_tag="VG709_07780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWC33012.1"
                     /translation="ERATAECSHIEQLLKESFERITALYELGADVTGVPSGFRDVDRL
                     TSGFQAGNLVIVAARPGMGKSARGLGIAANVAVRHRLPVALFTLEMSKAEVTQRIMCA
                     EAKVELHRLRTGRLSSDDWPRLTAACDRLAKAPIYVDDTGSITMMEIRSKARRLKSKH
                     PDLGLILVDYLQLMTSGSTAENRVQEVSQISRSLKVLARDLDVPIVAMSQLSRAVEQR
                     HDKRPILSDLRESGSIEQDADIVAFIYRDDYYNPEDSPDPGGAEVIVGKHRNGPTDKV
                     KLSFISQYAKFADLAAV"
     gene            929..>1541
                     /locus_tag="VG709_07785"
     CDS             929..>1541
                     /locus_tag="VG709_07785"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWC33013.1"
                     /translation="MPATVIVGAQWGDEGKGKVVDLLAEHSDVVCRYQGGPNAGHTIV
                     RRGETYKLHHVPSGILFEDKTCVVGAGCVVDPGLLVRELDELDARGISTAGLRVSGNA
                     HLSMPWHVAIDSASERRLGRLRIGTTRRGIGPAYADKAARIGIRVQDILDPKILRQKF
                     ETALAEKNVLLERMHGVEPLAVEDLADEMERYAERLRPYVADTS"
BASE COUNT          248 a          573 c          510 g          210 t
ORIGIN      
        1 gagcgcgcga ccgccgagtg cagccacatc gagcaactcc tgaaggagag cttcgagcgg
       61 atcaccgctc tctacgagct cggcgccgac gtgaccggcg tgccgtccgg gtttcgggac
      121 gtcgaccgcc tcacgtcggg cttccaggcg ggcaacctcg tgatcgtcgc ggcgcgcccc
      181 ggcatgggga agtcggcgcg cgggctcggc atcgcggcga acgtcgcggt ccgccatcgc
      241 ctccccgtcg ccctcttcac gctcgagatg tcgaaggccg aggtgacgca gcggatcatg
      301 tgcgccgagg cgaaggtcga gctccaccgc ctgcggacgg ggcgcctctc gtcggacgat
      361 tggccgcggc tcaccgccgc ctgcgaccgc ctcgccaagg cgccgatcta cgtcgacgac
      421 acgggctcga tcacgatgat ggagatccgc tccaaggccc gccgactcaa atcgaagcac
      481 ccggacctcg gcctcatcct cgtcgactac ctccagctca tgacctccgg atccacggca
      541 gagaaccgcg tccaggaggt gtcgcagatc tcgcgctcgc tcaaggtgct ggcccgcgac
      601 ctcgacgtgc cgatcgtggc catgtcgcag ctctcccgcg ccgtcgagca gcgccacgac
      661 aagcgcccga tcctctccga cctgcgcgag tcgggctcga tcgagcagga cgccgacatc
      721 gtcgcgttca tctaccgcga cgactactac aacccggagg actctcccga cccgggcggc
      781 gccgaggtca tcgtcgggaa gcaccgaaac ggcccgacgg acaaggtcaa gctctccttc
      841 atcagccagt acgcgaagtt cgccgacctc gccgcggtct agtcgcgagc gggccgcgcc
      901 gaccagcccg gcgtctaagc tccgtcgggt gcccgcgacg gtgatcgtcg gcgcgcagtg
      961 gggtgacgag gggaagggca aggtcgtcga cctgctcgcc gagcactcgg acgtcgtctg
     1021 ccgctaccag ggcggcccga acgcgggcca cacgatcgtc cgccgcgggg agacgtacaa
     1081 gctccaccac gtcccgtccg ggatcctctt cgaggacaag acctgcgtcg tcggagccgg
     1141 ctgcgtcgtc gacccgggac tgctcgtccg cgagctcgac gagctcgacg cgcgcggcat
     1201 ctcgaccgcc ggcctgcgcg tctcgggcaa cgcgcacctg agcatgccgt ggcacgtcgc
     1261 gatcgactcc gcgagcgagc gccgcctcgg ccggctccgg atcgggacga cgcggcgcgg
     1321 tatcggcccc gcctacgcgg acaaggcggc ccggatcggg atccgcgtcc aggacatcct
     1381 cgacccgaag atcctgcgcc agaagttcga gaccgccctc gccgagaaga acgtcctgct
     1441 cgagcggatg cacggcgtcg agccgctcgc cgtcgaggac ctcgcagacg agatggagcg
     1501 ctacgcggag cggctgcgcc cgtacgtcgc cgacacctcg c
//
LOCUS       DASZOB010000498         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17176, whole genome shotgun sequence.
ACCESSION   DASZOB010000498 DASZOB010000000
VERSION     DASZOB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17176"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VG709_07790"
     CDS             complement(<1..1983)
                     /locus_tag="VG709_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC33014.1"
                     /translation="MDARAFLDEVIAEEGVRDRLVHVETLPPRAPRPTPFTGLPAPLP
                     DRLRHLGVAGLYEHQRRALEILEDGRNVIVATGTASGKTLVYNLAFAKAALEDRRATA
                     LYLFPTKALARDQLRQIRSLRLPAIRAAVYDGDTPSPERPLIRRTANLILTNPDMLHA
                     AMLPGHAQWADFFLRLAFVVVDEAHVARGVFGSHVAMVLRRLRRLVARYRGEPRFVLA
                     SATIGNPRELAERLVGEPFEEIADDASPRGEKLFCLWNPPMVDDEAGIRRSAVSEASV
                     LLSRLVEREERAIAFARSRRSAELLADLTRRDVGGGLRERVKAYRAGYLPEERRAIER
                     SLSEGELLAVAATTALELGIDVGSLDAAVLAGYPGTRASMWQQAGRAGRRSESSLAFL
                     VAQDDPLDQYLVTHPDDLFRKPPEDAVVDPSNRHVVAPHLLCAAREQPLAREELGFFG
                     DAAYDVASTLVAEGRLVERRSLLHHRGDRPPHRDVDIRSASGRLFMIVEADTGALLGT
                     VDEARAYLQVHPGALYLHQGEQYEVAELDLGRHVALVRSVEADWYTQARTTIDIDVLA
                     ADEESKVGDTPMFFGTVRVTEQVVGYQRKQEGSGEVLENVELDLPPQVLETRSVWWAV
                     PDAVIGRAGVGAADLPGAAHAAEHAAIGLLPLVATCD"
BASE COUNT          293 a          756 c          752 g          264 t
ORIGIN      
        1 gtcgcacgtc gcgacgagcg gaagcagccc gatcgccgca tgctcggcgg cgtgcgcggc
       61 gccggggaga tcggcggccc cgacgcccgc gcggccgatc accgcgtcgg gaaccgccca
      121 ccataccgac cgggtctcga gcacctgcgg cgggagatcg agctcgacgt tctccaggac
      181 ctcgccggag ccctcctgct tgcgctggta gccgacgacc tgctcggtga cgcggaccgt
      241 cccgaagaac atgggtgtgt cgccgacctt ggactcctcg tcggcggcga gaacgtcgat
      301 gtcgatcgtc gtccgcgcct gcgtatacca gtcggcctcg accgagcgga ccagcgcgac
      361 gtgcctcccg agatcgagct ccgcgacctc gtactgctcc ccctggtgga ggtagagcgc
      421 gccgggatgc acctggaggt aggcgcgggc ctcgtcgacg gtaccgagca gcgcgccggt
      481 gtcggcctcc acgatcatga acagccgccc cgacgccgac cggatgtcga cgtctcgatg
      541 cggcggccgg tccccgcggt gatggagcag cgaccgccgt tcgacgagcc gtccctcggc
      601 gacgagcgtc gacgcgacgt cgtacgccgc gtccccgaag aacccgagct cctcgcgcgc
      661 aagcggctgt tcccgcgcag cgcacaggag gtgcggcgcg accacgtgcc ggttcgatgg
      721 gtcgacgacc gcgtcctccg gcggcttgcg gaacaggtcg tcggggtgcg tgacgaggta
      781 ctggtcgagc gggtcgtcct gcgcgacgag aaacgcgagc gacgactccg agcggcgccc
      841 ggcgcggccg gcctgctgcc acatcgacgc gcgcgtgccc gggtaccccg cgagcaccgc
      901 cgcgtcgagt gagccgacgt cgatcccgag ctcgagcgcg gtcgtcgccg cgaccgcgag
      961 cagctcgccc tccgagagcg agcgctcgat cgcgcgccgc tcctcgggca agtaaccggc
     1021 ccggtacgcc ttgacgcgct cgcggagccc cccgccgacg tcgcggcgcg tgaggtcggc
     1081 gaggagctcc gccgacctcc tcgacctcgc gaacgcgatc gcgcgctcct cgcgctcgac
     1141 gaggcgcgac agcaagaccg acgcctcgct caccgcgctg cgacggatcc ccgcctcgtc
     1201 gtcgaccatc ggcgggttcc acagacagaa gagcttctcg ccgcgcggcg aggcgtcgtc
     1261 cgcgatctcc tcgaacggct ccccgacgag ccgctccgcg agctcccggg ggttcccgat
     1321 cgtggccgaa gcgaggacga atcgcggttc gccgcggtac cgggcgacga gccgccggag
     1381 gcggcggagg accatcgcga cgtgggaccc gaacacgccg cgcgcgacgt gcgcctcgtc
     1441 gacgacgacg aacgcgagcc ggagaaagaa gtcggcccac tgcgcgtggc cggggagcat
     1501 cgcggcgtgg agcatgtcgg ggttcgtgag gatgaggttg gccgtgcgcc ggatcagcgg
     1561 acgctcgggc gacggcgtgt cgccgtcgta caccgcggca cggatcgccg ggagccgcag
     1621 cgagcggatc tggcggagct ggtcgcgcgc cagcgccttc gtcgggaaca ggtacagcgc
     1681 cgtcgcgcgc cggtcctcca gcgccgcctt cgcgaacgcg aggttgtaga cgagggtctt
     1741 cccgctcgcg gtcccggtcg cgacgatgac gttgcgcccg tcctcgagga tctcgagggc
     1801 ccgtcgctgg tgttcgtaga gcccggcgac gccgaggtgc cgtaggcggt cgggcagcgg
     1861 cgccggcagg ccggtgaagg gagtcggccg cggcgctcgc ggcgggagcg tctcgacgtg
     1921 gacgagacgg tcgcggacgc cctcctccgc gatcacctca tcgaggaacg cgcgcgcgtc
     1981 cacgcccgca tgttcccacg ccatccccag cgagccgccg cgtgagtcac cgcgaacgta
     2041 gcccttcgat cagggccgcc cggat
//
LOCUS       DASZOB010000499         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_37420, whole genome shotgun sequence.
ACCESSION   DASZOB010000499 DASZOB010000000
VERSION     DASZOB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37420"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..676)
                     /locus_tag="VG709_07795"
                     /pseudo
     CDS             complement(194..676)
                     /locus_tag="VG709_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            complement(712..1212)
                     /locus_tag="VG709_07800"
                     /pseudo
     CDS             complement(712..1212)
                     /locus_tag="VG709_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1269..1640)
                     /locus_tag="VG709_07805"
     CDS             complement(1269..1640)
                     /locus_tag="VG709_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWC33015.1"
                     /translation="MIVALADWFPFLVYGIFALAIPASMIAMSFLFATRPRRRPRART
                     VPFESGVSTGPPQRQRFTVSFYLTAMLFIVFDIEIVFLYPLAVVLQEFGWFALGAIAF
                     YLAMLIIPFVYDWKKGALEWR"
BASE COUNT          264 a          572 c          577 g          244 t
ORIGIN      
        1 cccgcgctcg ggatggcgat ggacgtggtc gacgcagacg gcaactcggt tcggggcgag
       61 gtcggcgagc tcgtgtgccg gaagccgttt cccgggatga cgcgcggctt ctggcgcgac
      121 cccgagcggt acctcgagac gccttccgca gcgggtggcc ttcccagtcc tcgtccatga
      181 tgatccgcac gaggttcggg tggtcgtcga tcgcgatgcc catcatgtcc cacgcttccc
      241 gctcgaacca gtcggcgctg ggccagatcg ggacgacgct cgccacgggg tcgccctcgt
      301 cggcccagac ctggaggcgg agccgcctcg gcgacgggcc gagcgcgagc aggtggtacg
      361 acatcgagaa gcgcttcggc ttcggctcgg gaagcttgtg caggccctgc gagctcggga
      421 agggcgcgtt gatgtcgcgc gcggacggct cccagccgcg cgtcgacgac tgcggagacg
      481 aggcccagta cccggcgacg cccgcgccgc cccagccgag gtagtcggcg gccgccacgt
      541 cggcgaggaa cgtgaagccg tgctcgtcgc gaaggtgccg tcccgcctcc cggagccgct
      601 cgagctccac gacgaccgtc gtctcgccgt acgcccgctt ctcctcgagg aagccgggga
      661 cgtcggcgta ggtcacgtgc gcgctctcgc cgtctcgtac gcgggcggaa cgcctgcctt
      721 gatcttctcc tgcagccgca cgatgccctc gagcacggct tccggacgcg gcgggcagcc
      781 ggggatgtag acgtccaccg gcacgatctt gtcgacgccc tggacgaccg cgtagttgtt
      841 gaacatcccg gccgagctcg cgcatgcgcc catcgcgatc acccacttgg gttccagcat
      901 ctggtcgtag atctgccgga acggcgccgc catcttgtgc gagaggcggc cggagacgat
      961 gagcaggtcc gcctggcgcg gcgagttccg gaacacctcg gcgccgaagc gcgcgacgtc
     1021 gtagcgcgcg gagacgatcg acatcatctc gatcgcacag caggcgaggc cgaaggtgtc
     1081 gggccagatc gagctgccct gcgcccacgc gacggccttc tcgaccgtcg tcagcaggag
     1141 cttgtcctcg atgtccggac cgaggtcctc cgcgaacttg ctcgggtcgc gccgcggccc
     1201 gcgcttctcc atcaggaggc gctccgtgtt gaggccgtag tcggcgagcc gcctgcggcc
     1261 ctcgcgctct accgccactc gagggccccc ttcttccagt cgtagacgaa ggggatgatc
     1321 agcatcgcga gatagaaggc gatcgccccg agcgcgaacc acccgaactc ctggagcacg
     1381 accgcgagcg ggtagaggaa gacgatctcg atgtcgaaga cgatgaagag catcgccgtg
     1441 aggtagaagc tgactgtgaa ccgctgccgc tgcggcggcc ccgtcgacac gccggactcg
     1501 aaggggaccg ttcgcgcgcg cggacgtctg cgcggccgtg tcgcgaagag gaacgacatc
     1561 gcgatcatcg aggcgggtat cgcgagcgcg aagatgccgt agacgaggaa cgggaaccag
     1621 tccgccaggg cgacgatcaa cgcgcccccc tcctacc
//
LOCUS       DASZOB010000500         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_139342, whole genome shotgun sequence.
ACCESSION   DASZOB010000500 DASZOB010000000
VERSION     DASZOB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139342"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VG709_07810"
     CDS             complement(<1..891)
                     /locus_tag="VG709_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWC33016.1"
                     /translation="MRYLALATDYDGTLAHHGEVAPEIVDALRRLKASGRRLILVTGR
                     ELDDLFDVFPDVDVFDRVVAENGALVYDPTTKDTRLLADGPPSGFAEELEKRGVGPIS
                     VGNVIVATWEPHETTVLETIREMGLELQVIFNKGAVMVLPSGINKATGLHDVLRELGL
                     SPHNVVGVGDAENDHAFLDLCECSVAVANALPSLQERCDHVTDGRHGDGVLELVEGLL
                     EDDLARIPPRRHRVPIGSVDGRDVWIEPYGSHVLVAGPSGSGKSSLTTTFLERLVDAG
                     YQFCLVDPEGDYQELSGAIVL"
     gene            complement(923..1471)
                     /locus_tag="VG709_07815"
     CDS             complement(923..1471)
                     /locus_tag="VG709_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWC33017.1"
                     /translation="MEELARNWWVLLVRGVLAVVFGVLALIWPGLALDVLVVLFGAFA
                     LVDGVFAIAAAIRAAERKRTWWPLVVEGVSGIALGIVAFVWPGITALALLYVIAAWAV
                     VTGVFEIAAAIRLRREIRGEWLLAFAGAASVAFGMLVAIFPGAGALAVVWAIGAYAIL
                     FGVLLVALGIRLRGFANEPARA"
BASE COUNT          283 a          617 c          553 g          200 t
ORIGIN      
        1 gaggacgatc gcgccggaga gctcctgata gtcgccttcg ggatcgacga ggcagaactg
       61 gtagccagcg tcgaccagcc gctcgaggaa cgtcgtcgtg agcgacgact tcccgctccc
      121 cgaaggcccc gcgacgagca cgtgcgagcc gtacggctcg atccagacgt cgcgtccgtc
      181 gacggaaccg atcggcacgc ggtgacgtcg cggtgggatc cgcgccaggt cgtcctcgag
      241 cagcccctcg acgagctcga ggacgccatc gccgtgccgc ccgtcggtca cgtggtcgca
      301 ccgctcctgg agcgacggga gcgcgttcgc gaccgcgacg gagcactcgc acaggtcgag
      361 gaacgcgtgg tcgttctcgg catcgcccac ccccacgacg ttgtgcggag acagcccgag
      421 ctctcggagg acgtcgtgga ggccggtcgc cttgttgatc cccgacggca gcaccatcac
      481 cgcgcccttg ttgaagatga cctggagctc gagccccatc tcgcggatcg tctcgagcac
      541 cgtcgtctcg tgcggctccc acgtcgcgac gatgacgttt ccgaccgaga taggaccgac
      601 gccccgcttc tcgagctctt ccgcgaaccc gctcggcggg ccgtcggcga gcaggcgggt
      661 gtccttggtc gtggggtcgt agaccagcgc gccgttctcg gcgaccaccc ggtcgaagac
      721 gtcgacgtcc gggaagacgt cgaagaggtc gtcgagctcc cgccccgtca ccaggatcag
      781 ccggcggccc gaggccttga gccgccggag cgcgtcgacg atctccggcg ccacctcgcc
      841 gtggtgggcg agcgtgccgt cgtaatcggt ggcgagcgcg agataccgca cgcgcactcc
      901 ttaccccacg gcggcggcgg gcctacgcgc gcgccggctc gttcgcgaac ccgcgcagcc
      961 ggatgccgag cgcgaccagc agcacgccga acaggatcgc gtacgcgccg atcgcccaga
     1021 cgaccgcgag cgcgccggca ccggggaaga tcgcgacgag catcccgaac gccacggagg
     1081 ccgccccggc gaacgcgagc agccattccc cgcggatctc ccgccgcaga cggatcgccg
     1141 ccgcgatctc gaatacgccg gtcacgacgg cccacgccgc gatcacgtag agcagcgcga
     1201 gcgcggtgat acccggccac acgaacgcga cgatcccgag cgcgataccg gagaccccct
     1261 cgacgaccag cggccaccac gtccgcttgc gctccgcagc acggatcgcg gcggcgatcg
     1321 cgaaaacgcc gtccacgaga gcgaaggcgc caaacagcac gacgaggacg tcgagcgcga
     1381 gaccgggcca gatcagcgcg aggacgccga agacgacggc gagcacgccc cgcacgagga
     1441 ggacccacca gttcctggcg agctcctcca cgcgtcgacc atactctccc ggatggagca
     1501 gaacgagccc cccgcccggc gcccgcggct cgagttcacg ccacgccgcg cgggcgacgt
     1561 cctcacgacc gggttcgagg tcttcggccg cggatggcga gtcctgctcg cgatcgccgc
     1621 gatcgtcacg atcccactga cgatcgcgaa gac
//
LOCUS       DASZOB010000501         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_37565, whole genome shotgun sequence.
ACCESSION   DASZOB010000501 DASZOB010000000
VERSION     DASZOB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37565"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VG709_07820"
     CDS             <1..678
                     /locus_tag="VG709_07820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWC33018.1"
                     /translation="VEAVKLSQLRRTIARRMSEAWQVPHFQIAMTADMRAAMRLRELL
                     VERMREGETKPTHSDVLTKACAAALMRHRDMNAHFAGDEVRLFPSADIGIAVAVERGL
                     LVPVIRGCERKTIAELAAARADVVERTRAGKIRQEDLEGGTFTISNLGMYGVDRFQAV
                     LNPPQAGILAVGAIAERAVVEDGEVAVRPIMEMTLACDHRAVDGATASEFLRSVRELL
                     EEPGLMA"
     gene            675..998
                     /locus_tag="VG709_07825"
     CDS             675..998
                     /locus_tag="VG709_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC33019.1"
                     /translation="MSVWYGVRDLEQARAFYVEKLGFTELYRDDDARWMRLARDTSEL
                     AVAEAPEETGAVLAVAVDDVKAEAERLREAGVEVGVVVEIPGTIRLLDVFDPDGNRLQ
                     LTQDL"
     gene            1142..2554
                     /gene="lpdA"
                     /locus_tag="VG709_07830"
     CDS             1142..2554
                     /gene="lpdA"
                     /locus_tag="VG709_07830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWC33020.1"
                     /translation="MECDVAVLGGGPGGYSAAIRAAQLGAKTVCVEKEPELGGTCLRV
                     GCIPTKAWVQTAYAIKEAEETFAKLGVNVGAPELDFAKANEWKAAVVTQLTGGVASLF
                     KANGVEWGKGVGRFSDANTIGVEDGEDVSFKTAIVATGSYPLRPPVDGIDSELCVDST
                     GLLAQSEVPKRLAILGGGIIGCEFASIFHRFGSEVTVIEMLPTLIPAEDADAQKELLK
                     QFKKRGIAVHLDSTCKRVEESGGGLTVHFGEGETVECDLMLVATGRGPLVEGLGLEEI
                     GVELDRKKGIAADEHRRTTVPHVYAVGDCAGYWQLAHTAFREGEVAAENATGHDAVVD
                     NRGVPRPIYTEPEVAAVGLTEQEAREQYGDDVATGTFPWSANGRAMMQNETVGWVKSI
                     HETRYGELLGIVMVGPHVTDLVEAGVVALDAESTVETIADGMAPHPTLSEAVKEAGLV
                     ALGRAIHFPNKKRRPAPTRA"
     gene            2646..>2840
                     /locus_tag="VG709_07835"
     CDS             2646..>2840
                     /locus_tag="VG709_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWC33021.1"
                     /translation="MAPLFAVKERPSVDGSFETVYRRHVRDVYGFALGLLGNASDAED
                     VAQTTFLNAYRARERGEDVEN"
BASE COUNT          457 a          957 c         1044 g          382 t
ORIGIN      
        1 gtcgaggcgg tgaagctgag ccaactccgc cggacgatcg cgcgcaggat gagcgaggcc
       61 tggcaggtac cgcatttcca gatcgcgatg acggccgaca tgcgcgccgc aatgcgcttg
      121 cgggagctgc tcgtcgagcg catgcgcgag ggcgagacga agccgacgca ctccgacgtc
      181 ctcacgaagg cctgcgccgc cgcgctgatg cgccaccgcg acatgaacgc gcacttcgcg
      241 ggcgacgagg tccggctctt cccgagcgcg gacatcggga tcgcggtcgc ggtcgagcgc
      301 ggcctcctcg tgcctgtgat ccgcggctgc gagcgcaaga cgatcgccga gctcgccgcc
      361 gctcgcgccg acgtcgtcga gcggacgcgc gcggggaaga ttcggcagga ggacctcgag
      421 ggaggcacgt tcacgatctc gaacctcggg atgtacggcg tcgaccgctt ccaggccgtc
      481 cttaacccgc cccaggccgg catcctcgcc gtcggcgcca tcgccgagcg cgcggtcgtc
      541 gaggacggcg aggtcgccgt caggccgatc atggagatga cgctcgcatg cgaccaccgg
      601 gccgtcgacg gcgcgacggc gtccgagttc ctccgctccg tcagggagct cctggaggag
      661 cccgggctca tggcatgagc gtctggtacg gcgttcgcga cctcgagcaa gcgcgcgcct
      721 tctacgtcga gaagctcggc ttcaccgagc tctaccgcga cgacgacgcc cgctggatgc
      781 ggctcgcgcg cgacacctcc gagctcgccg tggcggaggc tccggaggag accggcgccg
      841 tcctcgccgt ggcggtcgac gacgtgaagg cggaggccga gcgcctgcgc gaggcgggcg
      901 tcgaagtcgg ggtcgtcgtc gagattccgg ggacgatccg cctgcttgac gtcttcgacc
      961 ccgacggcaa caggctgcag ctgacgcagg acctgtagcg tcccgccccg cctgagtcgc
     1021 gggccggaga tacggtcgtg ccgtggggtc ggccgcccgt cgcggaagca cggcgtcccg
     1081 ctcggcacgc tgcgggctcg ggataggatc gtcggcgaca ccgaacccag gggacgcgcc
     1141 catggaatgt gacgtcgccg tcctcggcgg ggggccgggc ggctactcgg cggccatccg
     1201 cgccgcgcag ctcggagcca agaccgtctg cgtcgagaag gagcccgagc tcggcgggac
     1261 gtgcctgcgt gtcggctgca tcccgacgaa ggcgtgggtg cagaccgcgt acgcgatcaa
     1321 ggaggccgag gagacgttcg ccaagctcgg cgtgaacgtc ggagcgcccg agctcgactt
     1381 cgcgaaggcg aacgagtgga aagcggcagt ggtcacgcag ctgacgggcg gcgtcgccag
     1441 cctcttcaag gccaacggcg tcgagtgggg caagggcgtc ggccggttca gcgacgcgaa
     1501 cacgatcggg gtggaagacg gcgaggacgt ctcgttcaag acggcgatcg tggccacggg
     1561 gtcgtacccg ctgcggccgc ccgtggacgg catcgactcc gagctctgcg tcgactcgac
     1621 cggcctgctc gcgcagagcg aggtgccgaa gcgcctcgcg atcctcggcg gcggcatcat
     1681 cggatgcgag ttcgcctcga tcttccatcg cttcggaagc gaggtgaccg tcatcgagat
     1741 gctgccgacg ctgatcccgg cggaggacgc cgacgcgcag aaggagctcc tcaagcagtt
     1801 caagaagcga gggatcgccg tgcacctcga ctcgacctgc aagcgcgtcg aggagagcgg
     1861 cggcggcctc accgtccact tcggcgaggg cgagacggtc gagtgcgatc tcatgctcgt
     1921 cgcgaccggc cgcgggccgc tcgtggaggg cctcgggctc gaggagatcg gcgtcgagct
     1981 cgaccgcaag aaggggatcg cggccgacga gcaccgccgc acgaccgtcc ctcacgtcta
     2041 cgcggtcggc gactgcgccg gctactggca gctcgcgcac acggcgtttc gcgagggtga
     2101 ggtcgcggcc gagaacgcga cgggccacga cgcggtcgtc gacaaccgcg gcgtgccgcg
     2161 gccgatctac acggagcccg aggtggcggc ggtcgggctg accgagcagg aggcgcgcga
     2221 gcagtacggc gacgacgtcg cgacggggac cttcccgtgg tctgcgaacg ggcgcgcgat
     2281 gatgcagaac gagacggtcg gctgggtgaa gtcgatccac gagacgcgct acggcgagtt
     2341 gctcgggatc gtcatggtcg gcccgcacgt caccgacctc gtcgaggcgg gcgtcgtggc
     2401 gctcgacgcc gaatcgacgg tcgagacgat cgcggacgga atggcgccgc accccacgct
     2461 gtccgaggcg gtcaaggagg ccggcctcgt cgcgctcggg cgcgcgatcc acttcccgaa
     2521 caagaagcgg cgccccgcgc cgacccgcgc ctagccggag ggcgtttcta ctttcgcagc
     2581 accgcgcaag aaattggtgc gacgcacgtc tatgctgata caaggcgaat ccggtcgaga
     2641 gcgagatggc ccctctgttt gcggtaaagg aacgaccgag cgtcgacggc tcctttgaga
     2701 ccgtctaccg ccggcatgtc cgggacgtct acggtttcgc cctcgggctc ctcggcaacg
     2761 cctcggacgc cgaggacgtc gcccagacca cgttcctgaa cgcctaccgc gcccgcgagc
     2821 gcggggagga cgtggagaac
//
LOCUS       DASZOB010000502         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48034, whole genome shotgun sequence.
ACCESSION   DASZOB010000502 DASZOB010000000
VERSION     DASZOB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48034"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VG709_07840"
     CDS             complement(<1..204)
                     /locus_tag="VG709_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWC33022.1"
                     /translation="MVRHATVLVAEDEPDIRLLVRVNLTYAGLRVLEAKTGEEAVEIA
                     SREDPDVLLLDLRLPVWEGWEVLR"
     gene            330..878
                     /locus_tag="VG709_07845"
     CDS             330..878
                     /locus_tag="VG709_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33023.1"
                     /translation="MDQGRPKSRTDDAWSAVERAVRVVRPFGRAAERLAVAAFVAGAL
                     VWWAASRAGGSPLGLAVLGVILALPPLAVAAFAVAVRALVALPRKLREGRSALRGRAE
                     LIRDRAGEVVASRRRGPLQALRATVRLWWAVASSREVLEVLGPTTLLLSPATVVLAAI
                     GAVAAAFEILGGAVALIWLAVA"
     gene            complement(911..1144)
                     /locus_tag="VG709_07850"
     CDS             complement(911..1144)
                     /locus_tag="VG709_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33024.1"
                     /translation="MRGDRVEIVVDTGGGGVQRYEIEATRSGRRVEVATARGVVEVTE
                     VTRSGQPVRSARFMASRVIAVVESPAEGPSRRR"
     gene            complement(1205..2719)
                     /locus_tag="VG709_07855"
     CDS             complement(1205..2719)
                     /locus_tag="VG709_07855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWC33025.1"
                     /translation="MANGERQESHEYRQLVERMPVISYRDSVDPESSALYISPQVGPI
                     LGYAPDEWLQEPELWVRLLHPDDRDRVLSAHLATNRTGEPFEEEYRLIARDGRRVWVS
                     DRAMLVRDEAGRPQYWEGVMVDITDRKLAEERERRRSDYLAALHETALAALRRLDERT
                     MLETIVVDAAEVAGTDHAYAYVRVDEGLDMEVRAGTGVFRDWLGHRMSVGEGLAGRVT
                     ETGDPVVIDDYDAWEGRSPSFPGGILHAAIAVPLRSGPEIVGALGLAHLDPARRFSDD
                     DVERLAGFADVASVALENVRLYAALERELTQRRRVEEELAFLAYHDRLTELPNRAMFE
                     NVLELALARAERAGLAIAVVYLDLDDFKSVNDRLGHAAGDELLRALASRLRGAVRGTD
                     LIARHGGDEFLVLLADLEVDGAGGPTQARALAEAVARRLHDALTAPFGVAGEQLFVSA
                     SIGVSVYPLDSHDAKTLLRNSDAAMYRSKREGSGGTVFAAAPPAAPRPDERQNR"
     gene            2910..>3395
                     /locus_tag="VG709_07860"
     CDS             2910..>3395
                     /locus_tag="VG709_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HWC33026.1"
                     /translation="MSRWMALDTSSLAYRAFFALPSSIVAADGKPVHALHGYLDMTAR
                     LVREHRPDRILHALDDDWRPAPRVAAYAGYKAERPDVPPELAFQLDALREILVLAGMA
                     VISAPGWEAEDAIGTLAAGASDADRVDIVTGDRDLIQLVRDPVVRLLFTRRGVSDLEV
                     LD"
BASE COUNT          423 a         1264 c         1212 g          496 t
ORIGIN      
        1 cctcagcacc tcccacccct cccacaccgg gagacgcagg tcgagcagca ggacgtcggg
       61 gtcctcgcga gaggcgatct ccaccgcctc ttcgccggtc ttcgcctcga gcacgcggag
      121 ccccgcgtac gtgaggttga cccgcacgag gagccggatg tccggctcgt cctcggcgac
      181 caggaccgtc gcgtgccgca ccatgtgtca ccccttcgac ccgcaccggg gcgctcggcc
      241 agcacgcggt cggcgtgatt ctcgtgcggg tcgcgtgggg gcacaacccc ccaaccgggt
      301 catcttcgct gcgccgctac gatcggccgg tggatcaggg ccgaccgaag tcgcggacgg
      361 acgatgcctg gtcggccgtc gagcgcgccg tccgggtggt gcggccgttc ggcagggcgg
      421 ccgagcggct cgccgtcgcg gcgttcgtcg ccggcgcgct cgtgtggtgg gcggcgtccc
      481 gggccggcgg gagcccgctg gggctcgcgg tcctcggtgt gatcctggca ctcccgccgc
      541 tcgcggtcgc cgcgttcgcg gtcgcggtgc gcgccctcgt cgctcttccg cggaagctcc
      601 gtgagggtcg ttcggcgcta cgcgggcggg ccgagctcat ccgcgatcgc gccggcgagg
      661 tggtcgcgtc gcgccgccga gggccgctcc aggcgctccg cgcgacggtc aggctctggt
      721 gggcggtcgc ttcttcgcgc gaggtgctcg aggtgctcgg cccaacgacg ctcctgcttt
      781 cgccggcgac ggtcgtgctc gcggcgatcg gcgccgtcgc ggcggcgttc gagatcctcg
      841 gcggcgccgt cgcgctgatc tggctcgcgg tcgcgtagag cgcttcgctc gaggaagacg
      901 cggacgcgcc tcagcgacgc cgcgagggcc cctcggccgg cgattccacg acggcgatca
      961 cgcggctcgc catgaacctc gcgctgcgga ccggctggcc gctccgagtg acctcggtca
     1021 cctccacgac cccacgtgct gtcgccacct cgacgcggcg accggagcgc gtcgcctcta
     1081 tctcgtagcg ctgcacgccg cccccgccgg tgtcgacgac gatctcgacg cggtccccgc
     1141 gcatggcgcc ccccttcggt cctggccaat catgcgctgg cgcggggtcg gcgtcgagta
     1201 gcggtcagcg gttctgacgc tcgtcgggcc tcggcgcggc gggcggggcg gcggcgaaca
     1261 ccgtaccgcc ggacccctcg cgcttgcttc ggtacatcgc cgcgtcggag ttgcgcagca
     1321 acgtcttggc atcgtgcgag tcgagcggat acacgctgac gccgatgctc gccgacacga
     1381 acagctgctc gcccgcgacg ccgaacggag ccgtcagcgc atcgtgcagc cgacgcgcga
     1441 cggcctcggc caacgcgcgg gcctgcgtgg gtccgccggc cccgtcgacc tcgaggtccg
     1501 cgagcagcac gaggaactcg tcgccgccgt gccgcgcgat gaggtccgtc ccacggacgg
     1561 cgccgcggag cctcgatgca agtgcccgga gcagctcgtc ccccgcggcg tgtccgagcc
     1621 ggtcgttgac ggacttgaag tcgtcgagat cgaggtagac gacggcgatg gcgaggcctg
     1681 cccgttccgc acgggcgagg gcgagctcca gcacgttctc gaacatcgcg cggttcggga
     1741 gctcggtgag ccggtcgtga tacgcgagga acgcgagctc ctcctccacc cgccgccgct
     1801 gcgtgagctc gcgctcgagc gccgcgtaga gccgcacgtt ctcgagcgcg acggacgcga
     1861 catccgcgaa accggcgagc cgctcgacgt cgtcgtcgga gaagcgcctc gcgggatcga
     1921 ggtgcgcgag cccgagcgcg ccgacgatct cgggccccga gcgcagcggc accgcgatcg
     1981 ccgcatgaag gatgccgccc gggaacgacg gagaccgccc ctcccaggcg tcgtagtcgt
     2041 cgatcacgac ggggtcaccg gtctcggtga cgcggccggc gagaccctcc ccgaccgaca
     2101 tgcggtgtcc cagccagtcg cggaagaccc cggtcccggc gcgcacctcc atgtcgagtc
     2161 cctcgtcgac ccgcacatag gcgtaggcgt gatcggtgcc ggcgacctcg gcggcgtcga
     2221 cgacgatcgt ctcgagcatc gtccgctcgt cgaggcgccg gagcgcagcg agcgccgtct
     2281 cgtggagcgc cgctaggtag tcgctgcgcc gtcgctcgcg ttcctccgcg agcttgcggt
     2341 cggtgatgtc gaccatgacg ccctcccagt actgggggcg gccggcctcg tcgcggacga
     2401 gcatcgcgcg gtcgctgacc cagaccctcc tcccgtcgcg cgcgatcagg cggtactcct
     2461 cctcgaaggg ctcgccggtc cggttcgtcg cgagatgggc ggagaggacg cggtcgcggt
     2521 cgtcggggtg gaggaggcgc acccacagct ccggctcttg aagccactcg tcgggcgcgt
     2581 acccgaggat gggtccgacc tgtgggctga tgtagagcgc ggagctctcc ggatccacgg
     2641 agtcgcggta gctgatcacc ggcatgcgct cgacgagctg ccggtactcg tgcgactcct
     2701 ggcgttcgcc gttcgccatc tgctggtctc ctctacccgc ctcggcgctc gtccctacac
     2761 gggcggagcg ggggcagccg acggtggctc cccgagtatc ggacaaggcg gcaccaacct
     2821 tgaaccccac gcaagccggt catcgccgcg gcccgacgga tcgggggcgg cggcagccgg
     2881 cccccgcatc ggtgggagag gatgaccgcg tgagccgatg gatggccctc gatacgtcga
     2941 gcctcgcgta ccgggcgttc ttcgcgcttc cctcgtcgat cgtcgcggcc gacgggaaac
     3001 cggtccacgc cctccacggc tatctcgaca tgaccgcccg cctggtgcgg gagcatcggc
     3061 ccgaccggat cctccacgcg ctcgacgacg actggcggcc ggccccgagg gtcgccgcgt
     3121 acgccggcta caaggccgag cgtcccgacg tccccccgga gctcgccttc cagctcgacg
     3181 cgctgcggga gatcctcgtc ctcgccggca tggcggtgat ctccgccccg ggttgggagg
     3241 ccgaggacgc gatcggcacg ctcgcggccg gggcgtccga cgccgaccgc gtggacatcg
     3301 tcaccggcga ccgcgacctg atccagctcg tgcgcgaccc cgtcgtacgg ctgctcttca
     3361 ctcgccgcgg cgtctcggat ctcgaggtcc tcgac
//
LOCUS       DASZOB010000503         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_179564, whole genome shotgun sequence.
ACCESSION   DASZOB010000503 DASZOB010000000
VERSION     DASZOB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179564"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VG709_07865"
     CDS             <1..239
                     /locus_tag="VG709_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC33027.1"
                     /translation="NGRPFQAAAAIAKRHATETAVTAAREAVQVHGGYGFTEEFPVAR
                     HYRDAKVLEIGEGTSEVQRILIARDLGLPGTELA"
     gene            395..1528
                     /locus_tag="VG709_07870"
     CDS             395..1528
                     /locus_tag="VG709_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWC33028.1"
                     /translation="MLAQVTAAPVVAGPQQTKDRIEEARRRVKKLENEVREARSVLDG
                     IRADIASITAQLSQTLRQIEALGYRIDDTKNGIRRRGNRVDRLQSQIDARAMNAYMAG
                     PGGALELILNAHSLTDLSDRLTYLEVLNQRDADIATGLEVEREQLRRQKADLERYLAD
                     LEVLEEQQRDQAAGLHAKFEEQKELKESIERKLEATRNQIERLEKRYERERRALLARL
                     RAQRAAAAPIVGNAGPLPTADGPFYACPVDTPRSYIDDFGYPRSGGRSHQGNDIFAAT
                     GTPIRAPFAGRADESYNSLGGTSVHVYASDGSGDYVYNAHLSQHAGVDGQQVRPGDLI
                     GYVGNTGNAIYTAPHDHFEYHPGGGSAVSPYVYLNEVCGVGGG"
     gene            1565..>2826
                     /locus_tag="VG709_07875"
     CDS             1565..>2826
                     /locus_tag="VG709_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWC33029.1"
                     /translation="MTRDRPWIFRTYAGYATPRQTNERFKANLAKGQTGLSVAFDLPT
                     QCGYDSDDPMSVGEVGRVGVPIGHLGDMRDVFDGIPLADMNTSMTINATAVWLLALYV
                     AVADEHGAERGALAGTTQNDVLKEYLSRGTYAFPPEPSMRLTTDLVTWTYREVPKWNP
                     INVCSYHLQEAGAAPVLEIAFTLANACAVLDHVRESGRVSEDDLAGLVGRISFFCNAG
                     LRFVDEMCKMRAFVGLWDRLTAERYDVEDPKPRRFRYGVQVNSLGLTAHQPENNVYRI
                     LLETLAVTLSRDARARAIQLPAWNEALGLPRAWDQQWSLRAQQILAEETDVLEHDDLF
                     EGSRVVEDRTAEIAEAAWEELHRVLDMGGSVEALQYMKERLVAALAERQRRIETGEQT
                     VVGVNRYTEPEPSPLVEGLDEGFSAFHT"
BASE COUNT          475 a          992 c          986 g          373 t
ORIGIN      
        1 acaacggccg cccgttccag gcggcggccg cgatcgccaa gcgccacgct accgagacgg
       61 ccgtgacggc cgcgcgcgag gcggtccagg tccacggcgg ctacgggttc accgaggagt
      121 tcccggtggc gcgccactac cgcgatgcca aggtcctaga gatcggagag ggaaccagcg
      181 aggtccagcg gatcctcatc gcccgcgacc tggggctccc cggcaccgag ctcgcctagc
      241 gcccgcgacc gttttcaaca gtcacgctgg gaacgtcggt tgacagtttc accagcgtgc
      301 tgtacccata gcacccttcc ccatgagcgg agcgagccca gggtcgccac tccgccggct
      361 tcggcacgtc gccggagccg ttgtcgccgt cgccatgctg gcccaggtga cggcggcgcc
      421 ggtcgtcgcc ggcccgcagc agaccaagga ccgcatcgaa gaggcgcgcc ggcgggtcaa
      481 gaagctcgag aacgaggtgc gcgaggcgcg gtccgtgctc gacggcatcc gcgccgacat
      541 cgcgtcgatc accgcgcagc tttcccagac gctccgccag atcgaggcgc tcggataccg
      601 gatcgacgat acgaagaacg gcatccgccg gcgcggcaac cgagtcgacc ggctccagtc
      661 acagatcgac gcgcgggcga tgaacgcgta catggccgga cccggcggcg cgctcgagct
      721 gatcctcaat gcgcactcgc tcacggacct ctcggatcgc ctgacgtacc tggaggtcct
      781 gaaccagcgc gacgccgaca tcgcgacggg gctggaagtc gaacgcgaac agctacgccg
      841 ccagaaggcc gacctcgagc ggtacctcgc cgacctcgag gtcctcgagg agcagcagcg
      901 ggatcaggcc gccggcctac acgcgaagtt cgaggagcag aaggagctga aggagtcgat
      961 cgagcggaag ctcgaggcga cgaggaacca gatcgagcgg ctcgagaaga ggtacgagcg
     1021 agaacggagg gctctcctgg cgaggctccg tgcgcagcgg gccgccgcgg cgccgatcgt
     1081 cggcaacgcc ggaccgctcc cgaccgccga cgggccgttc tacgcgtgcc cggttgacac
     1141 gccgcggtcg tacatcgacg acttcgggta cccgcggtcg ggtggacgca gccaccaggg
     1201 caacgacatc ttcgcggcga ccgggacgcc gatccgcgcc ccgttcgcgg gccgcgccga
     1261 tgagagctac aactcgctcg gcgggacctc ggtccacgtc tacgccagcg acgggagcgg
     1321 cgactacgtc tacaacgcgc acctctcgca gcacgcgggc gtggacggcc agcaggtccg
     1381 accgggcgac ctcatcggat acgtcggcaa cacggggaac gcgatctata cggcgcccca
     1441 cgatcacttc gagtaccacc ccggcggcgg ctccgcggtg agcccgtacg tctacctcaa
     1501 cgaggtctgc ggggtcggcg gcggctagcc tcgcggggcc gcggggtatc ctccgggagg
     1561 cgccatgacc cgcgaccgtc cctggatctt ccgtacgtac gccggctacg cgacgccgcg
     1621 gcagacgaac gagcgcttca aggcgaatct cgcgaagggg caaaccgggc tctcggtcgc
     1681 gttcgacctc ccgacgcagt gcggctacga ctcggacgac ccgatgtcgg tgggcgaggt
     1741 cggtcgcgta ggcgttccga tcggacacct cggcgacatg cgggacgtct tcgacggcat
     1801 cccgctcgca gacatgaaca cctcgatgac gatcaacgcg acggccgtgt ggctcctcgc
     1861 gctctacgtc gcggtcgccg acgagcacgg cgctgagcgc ggcgcgctcg ccggcacgac
     1921 gcagaacgac gtcctcaagg agtacctctc gcgcgggacg tacgcgttcc cgcccgagcc
     1981 gtcgatgcgg ctcacgaccg atctcgtgac gtggacgtac cgggaggtcc cgaagtggaa
     2041 cccgatcaac gtctgctcgt accacctgca ggaggccggc gccgccccgg tgctggagat
     2101 cgcgttcacg ctcgccaacg cgtgcgcggt gctggaccac gtccgcgaga gcggccgcgt
     2161 gtccgaggac gacctcgccg gcctcgtcgg tcgcatctcg ttcttctgca acgccggcct
     2221 gcgcttcgtc gacgagatgt gcaagatgcg ggcgttcgtc gggctgtggg accggctcac
     2281 cgccgagcgg tacgacgtgg aggacccgaa gccccggcgg ttccgctacg gcgtccaggt
     2341 gaactcgctc ggacttacgg cgcaccagcc ggagaacaac gtctaccgga tcctgctcga
     2401 gacgctcgcg gtgacgctgt cgagggacgc gcgcgcacgc gcgatccagc tccccgcgtg
     2461 gaacgaggcg ctcgggctgc ctcgcgcgtg ggaccagcag tggtcgctac gtgcgcagca
     2521 gatcctggcg gaggagaccg acgtcctcga gcacgacgac ctcttcgagg gctcccgcgt
     2581 cgtcgaggac cgcacggccg agatcgccga ggcggcgtgg gaggagctgc accgcgtgct
     2641 cgacatgggc gggtcggtcg aggccctgca gtacatgaag gagcggctgg tcgcggccct
     2701 cgccgagcgg cagcgccgga tcgagaccgg cgagcagacc gtcgtcggcg tgaaccggta
     2761 cacggagccc gagccgtccc cgctcgtcga ggggctcgac gaggggttct cggcgttcca
     2821 cacgat
//
LOCUS       DASZOB010000504         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17485, whole genome shotgun sequence.
ACCESSION   DASZOB010000504 DASZOB010000000
VERSION     DASZOB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17485"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VG709_07880"
     CDS             complement(<1..571)
                     /locus_tag="VG709_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWC33030.1"
                     /translation="MVTQNHSHRLRRAARAAAEQGIDAVVVSPSADLLYLTGYDPPPL
                     ERLTCLLIRSDRDPTLVVPALERPLAESSGIATLVDLVHWNETDDPYRLVADRLGEAE
                     RVGCSERMWASHLLRLEEAVQRATFVPAQSVLAPLRAVKDVRELGLLRRAARAADETF
                     RRVVSMRLETMTERELATRLAELLVQMGHD"
     gene            612..>1614
                     /locus_tag="VG709_07885"
     CDS             612..>1614
                     /locus_tag="VG709_07885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWC33031.1"
                     /translation="MLEWSAATRERDLTAMADRRLDVLVIGGGITGAGIALDAASRGL
                     SIALVERDDFAAGTSGRSSRLIHGGVRYLRHGEFGLVYESLRERATLRRLAPHLVRPL
                     GFLVPTPEPSARMMTGIGLTLYDAVAVGRTVARHRRASPDEMRHLAPTLRHASGGYLS
                     FDCRTDDARLTLEILRQAVRHGALVANHAEVEAVLGQGRITGARVLDRITGRRLEIRA
                     EATINATGVWADAVCGLAFPSRSRLRPSKGVHVVIDRERLPIRSAVLFRSLAETSDFA
                     FAIPWGPRVYVGTTDTPYDGPLAEPRVEPQEVQLLLASLERAFEGDVGAAPVRASWAG
                     "
BASE COUNT          219 a          572 c          590 g          233 t
ORIGIN      
        1 cgtcgtgtcc catctggacc agcagctcgg cgagacgcgt cgcgagctcg cgttccgtca
       61 tggtctcgag gcgcatcgag acgacacgtc ggaacgtctc gtccgcggct cgggcggcac
      121 gccggaggag gcccagctca cggacgtcct tcactgcgcg gagcggcgcg agcacggact
      181 gcgccgggac gaacgtcgcc cgctgcacgg cctcctcgag acgcaggagg tgcgacgccc
      241 acatccgctc cgagcagccg acgcgttcag cttcgccgag gcgatccgcc acgaggcggt
      301 acgggtcgtc ggtctcgttc cagtggacga ggtcgacgag cgtcgcgatc ccggaggact
      361 ccgcgagcgg ccgctcgagt gcggggacga cgagggttgg gtcgcgatcc gaacggatca
      421 ataggcacgt cagccgttcg agcggcggcg gatcgtagcc cgtcaggtac aggaggtccg
      481 ccgagggcga cacgaccacg gcgtcgatcc cctgttcggc cgcggctctc gcggcgcgac
      541 ggagccggtg cgagtggttc tgggtgacca cggcgtcatg atacggaccg cgctgcggct
      601 accatccccg catgcttgag tggagcgccg ccacgcggga gcgcgatctg accgccatgg
      661 ccgaccggcg gctcgatgtc ctcgtgatcg gcggcgggat caccggtgcg ggcatcgcgc
      721 tcgacgccgc ctcgcgcggg ctctcgattg cgctcgtcga gcgagacgac ttcgcagcgg
      781 gaacgtcggg gcgctcatcg cggctcatcc acggaggcgt gcgctacctg cgccacggcg
      841 agttcggcct cgtctacgag tcgctccgag agcgcgcgac cctccgtcgg ctggcgccgc
      901 acctggtgcg tcctctcggg ttcctggtcc cgacgccgga gccctccgcg cggatgatga
      961 cggggatcgg gctcacgctg tacgacgccg tcgccgtcgg gcgcaccgtc gcccggcacc
     1021 gccgcgcgag tcccgacgag atgcggcatc tagcgccgac gctgcgccat gcatcgggcg
     1081 gctacctgtc gttcgactgc cgaacggacg acgcgaggtt gacgctcgag atcctccgtc
     1141 aagccgtgcg ccacggcgcg ctcgtcgcga accacgccga ggtagaggcg gttctcggcc
     1201 agggccggat caccggcgcc cgcgtcctgg accggatcac gggacgtcgc ctcgagatcc
     1261 gcgcagaggc gacgatcaac gccaccggtg tctgggccga cgcggtctgc gggcttgcct
     1321 tcccgagccg ctcgcggctc cgcccgagca agggggtcca cgtcgtgatc gaccgcgagc
     1381 gactgccgat ccgatcggcg gtcctgttcc ggtcgcttgc cgagacgtcg gatttcgcgt
     1441 tcgcgatccc gtggggccca cgggtctacg tcgggaccac cgacacgccg tacgacgggc
     1501 cgctcgccga gccgcgcgtc gagccgcagg aggtgcagct actgctcgcg tcgctcgagc
     1561 gcgcgttcga gggcgacgtc ggagcggcgc cggttcgcgc gagctgggcg ggcg
//
LOCUS       DASZOB010000505         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48093, whole genome shotgun sequence.
ACCESSION   DASZOB010000505 DASZOB010000000
VERSION     DASZOB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48093"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1283)
                     /gene="hisS"
                     /locus_tag="VG709_07890"
     CDS             complement(24..1283)
                     /gene="hisS"
                     /locus_tag="VG709_07890"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWC33032.1"
                     /translation="MTDFSPPRGTQDLLPPRSDRLEALADAAAALARLHGFRRVETPA
                     FEHTELFARGVGESTDIVTKEMYTFDDKGRRSLTLRPEGTAPVVRAYLGNRHDLPTPF
                     KAYYIVPMFRHERPQAGRMRQHTQFGVEIIGTDAPAADVEVISLAQRFLSEPSVGLEF
                     ALRINSIGDERCRPAYREALVGYLEERRDRLPKELRERLHINPLRVLDAKEDEVRDVT
                     ADAPRIIDFLDDECREHFERVRALLDDEGIAYEIDPRLVRGLDYYTRTTFEFVSDVLG
                     PTQSTLCGGGRYDGLAKVLGGDPTPGIGFGLGLERVLLAMERAGTVPDLSWTSVFVVA
                     LGDEAGRVGRELVRSLRRHGVRAETALEDRPLKAQLKMADRADAAYAALIGDDELAAK
                     EITMRRMSDGAQERVAIDEVHAWLASR"
     gene            complement(1280..1915)
                     /locus_tag="VG709_07895"
     CDS             complement(1280..1915)
                     /locus_tag="VG709_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWC33033.1"
                     /translation="MFLDVFDDNPFATNCWAVSADGSDAAVVVDPGFSPDRIRAMLDR
                     GGKRLDAVLATHGHADHIGSVQALCGDDVPLYIHEADELALTDQRAWGAGMPLDANLR
                     PAEVRTVRDGDVLEFAGFRIEVLHTPGHTPGHVCYRTDGFVFSGDLVFRGTIGRYDFP
                     NSSEDDMLASLRRFLTLPDPVPMYPGHGPDTTVGHERRTNEWLVSLAGTGE"
BASE COUNT          295 a          726 c          745 g          303 t
ORIGIN      
        1 gagcgcgccg gcgagatcgg ttctcagcgg ctcgcgagcc atgcgtggac ctcgtcgatc
       61 gcgacgcgtt cctgcgcgcc gtccgacatc cgccgcatcg tgatctcctt cgcagcgagc
      121 tcgtcgtcgc cgatcagcgc ggcgtaggcg gcgtcggcgc ggtcggccat cttgagctgc
      181 gccttgagcg ggcggtcctc gagcgccgtc tccgcgcgga cgccgtggcg gcgcagagac
      241 cggacgagct cccgcccgac gcgccccgcc tcgtcgccca gcgcgacgac gaacaccgac
      301 gtccacgaga gatccggcac ggtcccggcg cgctccatcg cgagcagcac gcgttcgagg
      361 cccagcccga acccgatccc gggcgtcggg tcgccaccga ggaccttcgc gagcccgtcg
      421 tagcgcccgc cgccgcagag cgtcgactgc gtcggcccga ggacgtcgct gacgaactcg
      481 aacgtcgtgc gcgtgtagta atcgaggccc cgcacgagcc gcgggtcgat ctcgtacgcg
      541 atgccctcgt cgtcgagcag ggcccgcacg cgctcgaagt gctcacggca ctcgtcgtcg
      601 agaaagtcga tgatgcgtgg cgcgtcggca gttacgtcac gcacctcgtc ctccttcgcg
      661 tccaggacgc gcagggggtt gatgtggagg cgctcgcgca gctccttcgg taggcggtca
      721 cggcgctcct cgaggtagcc gacgagcgct tcgcggtacg cgggacggca tcgctcgtcg
      781 ccgatgctgt tgatgcgaag cgcgaactcg agcccgaccg acggctccga caggaaccgc
      841 tgcgcgagcg agatcacctc gacgtcggcc gccggcgcgt cggtcccgat gatctcgacg
      901 ccgaactgcg tgtgctgacg catccggccg gcctgggggc gctcgtgccg gaacatcggg
      961 acgatgtagt acgccttgaa cggcgtgggg aggtcgtggc ggttcccgag gtacgcccgc
     1021 acgaccggcg ccgtgccctc cggccggagc gtaaggcttc ggcggccctt gtcgtcgaac
     1081 gtgtacatct ccttggtcac gatgtcggtc gactcgccga ccccgcgcgc gaacagctcg
     1141 gtgtgctcga acgccggcgt ctcgacgcgg cgaaagccgt ggagccgcgc gagcgccgcg
     1201 gcggcgtccg cgagcgcctc gagccggtcc gagcgaggag gcagcagatc ctgcgtcccc
     1261 cgcgggggcg agaagtccgt cactcccccg tcccggcgag cgagacgagc cactcgttgg
     1321 tccggcgctc gtgccccacg gtcgtgtcgg gcccgtgccc cgggtacatg gggaccggat
     1381 ccggaagcgt gaggaaccgc cgcaggctcg cgagcatgtc gtcctcgctc gagttcggga
     1441 agtcgtagcg tccgatcgtg cccctgaaga cgaggtcgcc gctgaacacg aacccgtcgg
     1501 tccggtagca gacgtgaccg ggcgtgtgcc ccggcgtgtg gagcacctcg atgcggaagc
     1561 ccgcgaactc gagcacgtcg ccgtcgcgga cggtccgcac ctcggccggc cggaggttcg
     1621 cgtcgagcgg catgccggct ccccacgctc gctggtcggt cagcgcgagc tcgtctgcct
     1681 cgtggatgta gagcggcacg tcgtctccgc agagcgcctg caccgacccg atgtggtcgg
     1741 cgtggccgtg ggtggcgagg acggcatcga gacgcttccc gccgcgatcg agcatcgcgc
     1801 ggatgcggtc gggggagaat cccgggtcga ccacgacggc ggcatccgag ccgtcggccg
     1861 agaccgccca gcagttggtc gcgaacggat tgtcgtcgaa gacgtcgagg aacatcgcgg
     1921 gccatcgtaa aggaaccgta tggcgttcca ccttcgagcc gtcgtactcc cggaggaacg
     1981 ggaacgcgac gtgttcgtcg agggcgatcg gttcgtcgcg gcaccggccg gcgtcgtcac
     2041 gacgctcggc gaggggctct gggcgaccc
//
LOCUS       DASZOB010000506         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_179647, whole genome shotgun sequence.
ACCESSION   DASZOB010000506 DASZOB010000000
VERSION     DASZOB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179647"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..356)
                     /locus_tag="VG709_07900"
     CDS             complement(153..356)
                     /locus_tag="VG709_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33034.1"
                     /translation="MASEHTSEAQEALAIEEADAWFEYLEAVRAHAPAGRYDEVEPWA
                     WARLTQRLHTVESERLRLRPAAA"
     gene            complement(409..1578)
                     /gene="rodA"
                     /locus_tag="VG709_07905"
     CDS             complement(409..1578)
                     /gene="rodA"
                     /locus_tag="VG709_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWC33035.1"
                     /translation="MAIRVEPQPTSSVSAEAAAIVRRLDFVLLAAVAGLVAYGLWVLG
                     AVSRNDVPGDPDYYVVRQSINVAVGTLAVAFVAFVNPEVYRSLRRLLYAVALIILAVV
                     FVADAIRDTQRWIEVGFFRFQPSEFGKVLLILVLAGFLADRARRLGEWRTTVAAVGLA
                     AVPTFLVFIEPDVGTAFVYCGILAAVLFIAGTRWSHLAALAVTALAAAASILWVLPSA
                     GVSVLRDYQLERLTGFVNPDLDPSGATYNITQSITAVGSGGLGGRGVAGATQTNMNYL
                     PEHATDFIFSSLAEQRGFFGAALLLLLYGLVVWRGVKTITVAPSLFTAMVAAGIVFTL
                     VFQVFVNVGMTIGIAPITGIPLPFVSYGGSSMVASLMMIGLLQAIHVRGRLAGRP"
     gene            complement(1592..3559)
                     /gene="mrdA"
                     /locus_tag="VG709_07910"
     CDS             complement(1592..3559)
                     /gene="mrdA"
                     /locus_tag="VG709_07910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWC33036.1"
                     /translation="MHSVPRFLPPDPRVEEPYRVTPQMAIRIAILGGVAIVLFAALFF
                     RLWALQVISGERYLEEARNNQVRTFRVQAPRGTISDRDGKLLVSNRTGTAVQLWPAAF
                     GDMPKEARTKVLVRLSKLLSLPVKDLNAALGRARNDPLTPVTVSEGVRGEKVAYLLEH
                     QAKFPGVQVAQTQLRRYEQGWLAAHVLGYVGEISQEQLDARARKGYAAGDRIGQAGGE
                     AADDTYLRGVPGVGEVRVDALGRVTSNRRYSQLPDAGYSVRLTIDADLQRAAEDALRF
                     GIRLAHAQDEWAANGGAVVAMDPHTGEILALASNPTFDPSIYVGRVHEKKLKRLADPA
                     ANHPTLNRAVAGLYPPGSTFKPVTALAAIQEGLLSPTEMIQCAPQRIVGEDKQVFANW
                     NPFVNEPMELTTAIANSCDTYFYEVALRAYERPDSPIQTWARRMGFGQATGIEVGPEG
                     TGLMPTPAWRKRTFKSPIEKIWTSGDSVQLGIGQGDVLATPLQMTRFSALVANGGKLV
                     EPTLVSRIEHASADGDAAVVVRRFASPRPKDIGLDPTAVRFVQEGLYDATHAPYGTST
                     SVFGAFPRPIAGKTVTAEKYVELPGYKGLRDQSWWCGYGPYGDPELVVCAVIENGGHG
                     GEAAAPTALKVFERFFGVESGTYPTSAVETD"
     gene            complement(3552..>3873)
                     /locus_tag="VG709_07915"
     CDS             complement(3552..>3873)
                     /locus_tag="VG709_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33037.1"
                     /translation="VAIALLRGPLLGAVAGFWAGRFGEATSRSSPHPPLVAAALATVG
                     VVVGSGVLHFMLGQGASAADLLGQVLVPTLALNLLLAYPAYRISLRVFPVAGREPTEA
                     TARA"
BASE COUNT          614 a         1353 c         1377 g          529 t
ORIGIN      
        1 actcggcgcg cggggcactc aacggggatg catgctcaaa tctcaatgcg atcagaacgt
       61 cggaagtccg gccgccggag ggcgctcggt gcttccgcac cgttgtccac agcctagcag
      121 cggccgtgga ggatcccggc ccggcccggc cgctacgccg ccgcgggccg aagacgcaac
      181 cgctcgctct cgacggtgtg aaggcgctgc gtgaggcgcg cccacgccca cggctcgacc
      241 tcgtcgtagc ggcccgccgg cgcgtgcgcg cggacggcct cgaggtactc gaaccaggcg
      301 tcggcctcct cgatcgcgag cgcctcctgc gcctcgctcg tgtgctcgct cgccatcgcc
      361 ccgaaggcta ggaccggcgc cggacggacc gccggaaggg cagcgccgtc agggacggcc
      421 ggcgagccgg ccgcgcacgt ggatcgcctg gaggagcccg atcatcatca ggctcgccac
      481 catcgagctt ccgccgtagc tcacgaacgg aagcgggatc cccgtgatcg gagcgattcc
      541 gatcgtcatg ccgacgttca cgaacacctg gaagacgagc gtgaagacga tcccggcggc
      601 caccatcgcg gtgaagaggc tcggcgccac ggtgatcgtc ttgaccccgc gccagacgac
      661 cagcccgtac aggagaagca ggagcgcggc accgaagaag ccgcgctgct cggcgagcga
      721 cgagaagatg aagtcggtcg cgtgctccgg gaggtagttc atgttcgtct gcgtggcgcc
      781 cgcgacgccc cgccccccga gcccccccga gccgacggca gtgatcgact gggtgatgtt
      841 gtacgtggca ccgctcgggt cgaggtcagg gttcacgaat cccgtcagcc gctcgagctg
      901 gtagtcgcgg agcactgaga cccccgccga gggaagcacc cagaggatcg acgcggccgc
      961 ggcgagggcg gtgacggcga gcgccgcgag atgcgaccac cgcgtcccgg cgatgaagag
     1021 gacggcggcg aggatcccgc agtacacgaa ggccgtcccg acgtcgggct cgatgaagac
     1081 gaggaaggtg gggactgccg cgaggccgac ggcggcgacc gtggtccgcc attccccgag
     1141 ccgccgcgcc cggtcggcga ggaagccggc gaggacgagg atgagcaaca ccttgccgaa
     1201 ctcggagggc tggaagcgga agaagccgac ctcgatccac cgctgggtgt cgcgaatcgc
     1261 gtcggcgacg aagacgacgg cgaggatgat cagcgccacg gcgtagagca ggcggcggag
     1321 cgagcgatag acctccgggt tgacgaacgc gacgaaggcg accgccagcg tgccgaccgc
     1381 cacgttgatg ctctggcgga cgacgtagta gtcgggatcg cccggcacgt cgtttcgtga
     1441 gaccgcgcca agaacccaga gcccgtacgc gacaaggcct gccacggccg cgaggaggac
     1501 gaagtcgagc cggcggacga tcgccgcggc ctcggcgctc acggacgagg tcggctgagg
     1561 ttcgacgcgg atcgccatca gaggtccact ttcagtcggt ctccacggca ctcgtcggat
     1621 acgtgccgga ctcgactccg aagaaccgct cgaacacctt gagagccgtg ggcgcggccg
     1681 cctccccgcc gtggccgccg ttctcgatga cggcgcagac gacgagctcg gggtcgccgt
     1741 acggcccgta gccgcaccac cacgactggt cgcgaagccc cttgtatccg ggcagctcga
     1801 cgtacttctc ggccgtcacg gtcttgccgg cgatcgggcg cgggaatgcc ccgaagacgc
     1861 tcgtcgacgt cccgtagggc gcgtgcgtcg cgtcgtagag tccctcctgg acgaaccgga
     1921 cggcggtcgg atcgagtccg atgtccttcg gcctcggcga tgcgaaccgc cgcacgacga
     1981 ccgccgcgtc gccgtcggcg ctcgcatgct cgatgcggct caccagcgtc ggctcgacga
     2041 gcttgccgcc gttcgcgacg agcgcggaga agcgcgtcat ctgaagcggc gtcgcgagca
     2101 cgtcgccctg cccgatcccg agctgcacgg agtcgccgct cgtccagatc ttctcgatcg
     2161 ggctcttgaa cgtccgcttg cgccacgccg gagtcggcat gaggccggtt ccctcggggc
     2221 cgacctcgat cccggtcgcc tgcccgaacc ccatgcggcg agcccacgtc tggatgggcg
     2281 agtcgggacg ctcgtacgcc cgcagggcga cctcgtagaa gtacgtgtcg cacgagttcg
     2341 cgatcgccgt cgtgagctcc atcggctcgt tcacgaacgg gttccagttc gcgaagacct
     2401 gcttgtcctc gccgacgatc cgctgcggcg cgcactggat catctccgta gggctcagca
     2461 acccctcctg gatcgccgcg agcgcggtca cgggcttgaa ggtcgagccg ggcggataga
     2521 ggccggcgac cgcgcggttc agcgtcgggt ggttcgccgc cgggtcggcg aggcgcttca
     2581 gcttcttctc atggacgcgc ccgacgtaga tcgacgggtc gaacgtcggg ttggaggcga
     2641 gcgcgaggat ctcgcccgtg tgcgggtcca tcgccacaac cgccccgccg ttcgcggccc
     2701 attcgtcctg cgcgtgcgcg aggcggatcc cgaagcgaag cgcgtcctcg gcggcacgct
     2761 gcaggtcggc gtcgatcgtc aggcgaaccg agtaccccgc gtcggggagc tggctgtagc
     2821 gccggttgct cgtgacgcgc ccgagcgcgt cgacacgaac ctctccgact cccggaacgc
     2881 cgcgaaggta cgtgtcgtcc gccgcctcgc cgccggcctg gccgatcctg tctcccgccg
     2941 catagccctt gcgcgcccgt gcgtcgagct gctcctgcga gatctcgccg acgtagccga
     3001 gaacgtgtgc cgcgagccag ccctgctcgt accgtcgaag ctgcgtctgg gcgacctgga
     3061 cgccggggaa cttcgcctgg tgctcgagca ggtaggcgac cttctcgccc cggaccccct
     3121 cgctgaccgt caccggcgtg agcgggtcgt tccgggctcg tccgagggcc gcgttcaggt
     3181 ccttcaccgg gaggctcagg agcttcgaga gccggacgag gaccttcgtc cgcgcctcct
     3241 tcggcatgtc gccgaacgcc gcaggccaga gctgaaccgc cgttcccgtc ctgttcgaga
     3301 cgaggagctt gccgtcccga tcggagatcg tcccccgcgg tgcctggacg cggaaggtgc
     3361 ggacctggtt gttccgcgcc tcctcgaggt accgctcgcc ggagatgacc tggagcgccc
     3421 agaggcggaa gaagagcgcc gcgaagagca cgatcgcgac gccgccgaga atcgcgatcc
     3481 ggatcgccat ctggggagtt acccggtacg gctcctcgac gcgtgggtcg ggaggcagga
     3541 agcgcggaac gctatgcacg cgccgtcgcc tccgttggct cgcggcccgc gaccgggaag
     3601 acgcggagcg agatgcggta cgccggatag gcgagcaaga ggttgagcgc cagggtcgga
     3661 acgaggacct gcccgagcag gtcggcggcg gaggccccct ggccgagcat gaagtggagc
     3721 accccggagc cgacgaccac gccgaccgtg gcgagcgccg ccgccacgag cggcgggtgc
     3781 ggcgagctcc tgctcgtcgc ctcgccgaag cgcccggccc agaagccggc gaccgctccg
     3841 aggagcgggc cgcgcagaag cgcgatcgcg acg
//
LOCUS       DASZOB010000507         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_139590, whole genome shotgun sequence.
ACCESSION   DASZOB010000507 DASZOB010000000
VERSION     DASZOB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139590"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..655)
                     /locus_tag="VG709_07920"
     CDS             complement(56..655)
                     /locus_tag="VG709_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33038.1"
                     /translation="MTDGSDAGPRTRVASERADEPQRAPAALAHPAAVLATLLVIVLA
                     MSWQFIADASRAVPAFDTAFYQWRAELVQHGEPGLLITLRGATGALAGGYRVAEAVLG
                     GLMRTVGGVGPETHTVVLSVMFRVLAGLGLAAFAWQRRRNWLLFYLTLVSVPALFLLQ
                     RFFGYLDNFLTLSLLVGVLLLIEPMRWSRNSAGTDTSVR"
     gene            complement(652..>1585)
                     /locus_tag="VG709_07925"
     CDS             complement(652..>1585)
                     /locus_tag="VG709_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWC33039.1"
                     /translation="RADLARVNLRPAMVDLYERARDNEPVVGMIGSLGAIGMIGRGAG
                     FPGGDRDVVAGQTLDGWRLSPSGRPLYRFPRYVRSVPGLARELRRLDLADGKLDRSWR
                     GRPFPPDVSELPAFAPYQTSVVRELIRRERFGRDRVPDFLFVNYKQIDKIGHRSSMNS
                     SEMAAAVRSSDAALGTIVRMLDREVGRGRWVLALTADHGATPDRSVTGGPALDTQDLY
                     ADLLARFDRDGDDVPAIQSARLTQIWVDETELAENGFEVEDVARFLARYRVSDNAGSG
                     MPAGRPDRTAFTAAFPSEVLKDLACLRDTKRRNG"
BASE COUNT          233 a          572 c          561 g          219 t
ORIGIN      
        1 cggggctcgg gctcgccgcg ttcgcgtggc agcgccgccg caactggctc ctcttctacc
       61 tgacgctcgt gtcggtcccg gcactgttcc tgctccatcg catcggctcg atcagcagca
      121 ggacgccgac caacagcgag agcgtgagga agttgtccag gtacccgaag aaccgctgga
      181 gcaggaacag tgccgggacc gacacgagcg tcaggtagaa gaggagccag ttgcggcggc
      241 gctgccacgc gaacgcggcg agcccgagcc ccgcgagcac gcggaacatc accgagagga
      301 cgacggtgtg cgtctcgggt ccgacgccgc caaccgtcct catgaggcca ccgaggaccg
      361 cctcggcgac gcggtacccg ccggccagcg cgccggtggc gccgcgcagc gtgatcagca
      421 gtccgggctc gccgtgctgc acgagctccg cgcgccactg gtagaacgcc gtgtcgaacg
      481 ccgggaccgc tcgtgacgcg tcggcgatga actgccacga catcgcgagc acgatcacga
      541 gcagcgtcgc gagaaccgcg gccgggtgcg ccagtgccgc cggcgcacgc tgcggttcgt
      601 cggcgcgctc ggacgcgacg cgggtgcggg gtccggcgtc gctcccgtcc gtcatccgtt
      661 cctcctcttc gtgtctcgaa gacaggccag gtccttcagc acctcgctcg ggaaggcggc
      721 cgtgaacgca gtccggtccg gccgacccgc gggcatgccg ctcccggcat tgtccgacac
      781 gcggtaccgg gcgaggaagc gagcgacatc ttcgacctcg aagccgtttt cggccagttc
      841 cgtctcgtcg acccagatct gcgtgaggcg ggcgctctgg atggcgggga cgtcgtcgcc
      901 gtcgcggtcg aaccgggcca ggaggtccgc gtacaggtcc tgggtgtcca gcgcgggtcc
      961 accggtgacg ctcctgtccg gcgtcgcccc gtggtcggcg gtcagcgcga ggacccagcg
     1021 ccctcgtccg acctcgcggt cgagcatccg aacgatcgtg ccgagcgccg cgtcgctcga
     1081 ccgcaccgcg gccgccatct cggaggagtt catgctcgat cggtggccga tcttgtcgat
     1141 ctgcttgtag ttcacgaaca ggaagtccgg cacgcggtcg cgtccgaagc gctcgcgacg
     1201 gatcagctcg cgcacgacgg acgtctggta cggcgcgaac gccggcagct ccgagacgtc
     1261 cggagggaac gggcgccctc tccacgagcg gtcgagcttg ccgtccgcga ggtcgagacg
     1321 ccggagctcg cgcgccagcc cggggacgct gcggacgtac cgcgggaagc ggtacagggg
     1381 ccgccccgag gggctgaggc gccaaccgtc gagcgtctgc cccgcgacga cgtcccgatc
     1441 tcctccgggg aacccggcgc cccgcccgat catgccgatc gcgccgagcg agccgatcat
     1501 gccgacgacc ggctcgttgt cgcgcgcccg ctcgtacagg tccaccatcg cggggcgcag
     1561 gttgaccctc gcgaggtcgg cgcgt
//
LOCUS       DASZOB010000508         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17551, whole genome shotgun sequence.
ACCESSION   DASZOB010000508 DASZOB010000000
VERSION     DASZOB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17551"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..929)
                     /locus_tag="VG709_07930"
     CDS             complement(78..929)
                     /locus_tag="VG709_07930"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWC33040.1"
                     /translation="MRIGLVVHGGRESALRAAADVVGWCGERGLKTRSLEVDVAADEV
                     VANDRFPEDLDLVVSVGGDGTLLRAALMGYRADVPVLGVKAGRMGFLTEVEPEEAPEA
                     LEAWIAGELEVEERMALVAEAEGAPWGEPQWALNEVIVEKRARHRVVRLAATVGDDYV
                     MTFSADGIIVATPTGSTAYSFSARGPIVSPQLRCLVLAPVSAHMVFDRPVVLAPHERV
                     TLEVREDEPGMLSADGRPGLELPVGSRVHVRRADRPARLLRSKRTESFYSLLRRKFSL
                     PAEPTGA"
     gene            complement(926..1717)
                     /locus_tag="VG709_07935"
     CDS             complement(926..1717)
                     /locus_tag="VG709_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWC33041.1"
                     /translation="MTRRRLDLELVRRGLAGSRTEALSLIASGRVRVGGTPAVGHRRL
                     VAAQESVSVARPPRRYASRGGDKLAGALDAFRVDPGGKRCLDAGAATGGFTDVLLSRG
                     AAHVVAVDVSYGELAWRLRRDRRVTVLERVNVRALTSDDLPYEPELVVADLSFISLRV
                     VVGTLVTLSSGGADLMLLVKPQFEASPEEVEPGGVVRDPHVWRRVLEGVGEAAASAGA
                     GTLGAVGSAVRGPAGNVEFFLHARVGSPSRDVDLGAVVSGIEEAS"
     gene            complement(1714..2217)
                     /locus_tag="VG709_07940"
     CDS             complement(1714..2217)
                     /locus_tag="VG709_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33042.1"
                     /translation="MRDTVRRYVEAGREALTPQKAEQLARSLVRQGAARKDQVQQVAR
                     ELRQWSRQNSERLVSIIQREVKRQVENVGVATKDDVEALKRRVRDLERSSSRKPAGAK
                     PSAAKPAGAKASGQKKSGQKTSGQKTSGRKTSAAKTSGGKRTGTRKPTTPAPAAPPPP
                     PVAPPTA"
     gene            2335..>2675
                     /locus_tag="VG709_07945"
     CDS             2335..>2675
                     /locus_tag="VG709_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33043.1"
                     /translation="MKRSRRFLAFSAAALAAAATAGRVVARRDGRREGASDEEHLGSI
                     RGEPSVVRGPRASRIYTERFDARPGGSGTLLFTHGYCLTEALWHYQKRDLAGGRHAIA
                     TWDLPGHGHSPP"
BASE COUNT          375 a         1040 c          896 g          364 t
ORIGIN      
        1 accacgaaga ctcccgcagt gaccttcatc cagtacgcga ccggtggcca gcgacgctgc
       61 cactcgggcg cggccatcta cgctcccgtg ggctcggcgg ggagcgagaa cttgcggcgc
      121 agcagcgagt agaacgactc cgtgcgcttg ctccgcagca gccgcgccgg acggtccgcg
      181 cgccggacgt ggacgcgcga gccgaccggc agctcgagtc cgggacgccc gtccgccgag
      241 agcatccccg gctcgtcttc gcgaacctcg agcgtgaccc gttcgtgcgg agccagcacg
      301 accggacgat cgaacaccat gtgcgcggat accggcgcga gcacgaggca acgcagctgc
      361 ggggagacga tcggcccacg cgcggagaag gagtacgccg tcgatcccgt cggcgtcgcg
      421 acgatgatgc cgtcggctga gaacgtcatg acgtagtcgt cgcctaccgt cgccgcgagc
      481 cggacgacgc ggtgccgcgc gcgcttctcg acgatgacct cattcagcgc ccactgcggc
      541 tcgccccatg gagcgccctc ggcctccgcg acgagcgcca tccgctcctc gacctcgagc
      601 tccccggcga tccacgcctc gagcgcctca ggcgcctcct cgggttccac ctcggtcagg
      661 aagcccatcc gtccagcctt gactccgagc accgggacgt cggcccggta ccccatgagc
      721 gccgcgcgca gcaacgttcc gtcgccgccg accgagacga cgagatcgag atcctccggg
      781 aaccggtcgt tcgctacgac ctcatcggcc gccacgtcga cctcgagcga gcgcgtcttg
      841 agcccgcgtt ccccgcacca cccgacgacg tccgccgcgg cgcgcagggc ggactcgcgc
      901 ccaccgtgga cgacgagccc gatcctcacg aggcctcctc gatacccgag acgacggctc
      961 cgagatcgac gtcgcgcgaa gggctgccga cccgcgcgtg caggaagaac tcgacgttgc
     1021 cggcgggacc gcggacggcc gacccgaccg cgccgagcgt gccggctccg gccgacgccg
     1081 cggcctcccc gaccccctcg aggacccttc gccacacgtg ggggtcgcgg acgacgcctc
     1141 ccggctcgac ctcctcaggc gacgcctcga actgcggctt caccagcagc atgaggtcgg
     1201 cgccgcccga cgacaacgtg accagtgtcc ccacgacgac gcggagcgag atgaacgaga
     1261 gatcggcgac gaccagctcc ggctcgtacg ggaggtcatc cgaggtcagc gcccgaacgt
     1321 tgacgcgctc gaggacggtc actcggcgat cgcgccgcag ccgccacgcg agctcgccgt
     1381 acgagacgtc gacggcgacg acgtgcgcgg cgccccgtga tagcaggacg tcggtgaagc
     1441 cgcctgtcgc ggcgccggca tcgagacagc gcttcccccc cggatcgacc cggaacgcgt
     1501 cgagcgcgcc cgcgagcttg tcgccgccgc gcgacgcgta tcgccggggc gggcgcgcga
     1561 cggacacgga ctcttgcgcc gccacgagcc ggcggtggcc gaccgcaggc gtgccgccga
     1621 cgcgaacacg ccccgacgcg atcaacgaga gcgcctcggt cctcgacccc gcgagccccc
     1681 ttcgcaccag ctcgaggtcg aggcgccgcc gcgtcacgcc gtgggcggcg cgaccggcgg
     1741 aggcggcggg gcggccggcg ccggcgtcgt cggcttgcgc gttcccgtgc gcttgccgcc
     1801 ggaggtcttg gccgcggacg tcttccgtcc ggacgtcttc tgtccggacg tcttctgtcc
     1861 ggacttcttc tgtcccgacg ccttggcgcc cgccggcttc gccgccgacg gcttcgcgcc
     1921 ggcgggcttg cggctcgagg accgctcgag gtcgcgcacc cgccgcttca gcgcctcgac
     1981 gtcgtccttc gtcgcgacgc cgacgttctc gacctgccgc ttcacctcgc gctggatgat
     2041 cgacacgaga cgctcgctgt tctgacgcga ccactgccga agctcgcggg cgacctgctg
     2101 gacctggtcc ttgcgcgccg cgccctggcg gacgagcgag cgcgcgagct gttcagcctt
     2161 ctgcggcgtg agcgcctcgc gccccgcctc cacatacctg cgtacggtgt cgcgcatcgg
     2221 tacctccgtt ccgtcttttc gcgaaggata gtcgctcgac gcggctataa tccggccgct
     2281 tccccgtcgt ctcgcagccg accgtcggcc tccggaggtg acaccgtgcc ccgcgtgaag
     2341 cgctcccgcc ggttccttgc gttctcggcc gctgcgctcg cggcggcagc caccgccggg
     2401 cgggtcgtgg cgcgccggga cggacgccgc gagggcgcgt ccgacgagga gcacctcgga
     2461 tcgatccgcg gcgagccctc cgtcgtgcga ggaccccgtg cctccaggat ctacaccgag
     2521 cggttcgacg cacggcccgg cggatccggg acactgctat tcacgcatgg ctactgcctg
     2581 accgaggctc tctggcatta tcagaagcgc gatctcgccg gcgggaggca cgctatcgcg
     2641 acctgggacc tgccgggcca cggtcactct cctcc
//
LOCUS       DASZOB010000509         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_190550, whole genome shotgun sequence.
ACCESSION   DASZOB010000509 DASZOB010000000
VERSION     DASZOB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190550"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..582
                     /locus_tag="VG709_07950"
                     /pseudo
     CDS             1..582
                     /locus_tag="VG709_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(671..835)
                     /gene="rpmG"
                     /locus_tag="VG709_07955"
     CDS             complement(671..835)
                     /gene="rpmG"
                     /locus_tag="VG709_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWC33044.1"
                     /translation="MPKSENRPKITLACEECKRRNYLTEKNRVNDRDRIGLRKYCRWC
                     RRHTLHRETR"
     gene            complement(836..2020)
                     /gene="tuf"
                     /locus_tag="VG709_07960"
     CDS             complement(836..2020)
                     /gene="tuf"
                     /locus_tag="VG709_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWC33045.1"
                     /translation="MSKKKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLAERGMADY
                     TPFEQIDKAPEEKERGITIAIAHVEYETERRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPPTREHVLLARQVNVPSIVVALNKVDAVDDPELLDLVELEVRELLNQ
                     YEFPGDDTPVVRVSALKALEGDEEARGQILELMEACDTYIPEPTRDVDRPFLMPIEDV
                     FSITGRGTVVTGRVERGTLKKMGEVAIVGIHDTKKTVATDLEMFRKLLDEVQAGDNVG
                     VLLRGIDKDDVARGQVLAAPGSITPHTDFEAQVYVLSKDEGGRHTPFLNGYRPQFYFR
                     TTDVTGAIQLPDGVEMVMPGDNVTMQVSMIAPIAMEEGLRFAIREGGRTVGAGRVTNI
                     QK"
     gene            complement(2036..4114)
                     /gene="fusA"
                     /locus_tag="VG709_07965"
     CDS             complement(2036..4114)
                     /gene="fusA"
                     /locus_tag="VG709_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWC33046.1"
                     /translation="MREYPLQRTRNIGIMAHIDAGKTTTPERILYYTGKTYKVGEVHE
                     GTAVMDWMVQEQERGITITSAATTCHWKNHWINIIDTPGHVDFTVEVERSLRVLDGAV
                     AIFDAVAGVEPQSETVWRQADRYRVPRICFVNKMDRVGADFERTVGMIEDRLNAMPLV
                     VQLPWGIEESFTGVLDLVAMKGLRWSSEKGEEWETVEIPDDLRETALEWRHRLYEQLA
                     DHHEGLMEKYVAGEEPPPEELHQALRDATLAGEGTPVLCGTAFKNKGIQPLLDAVVDY
                     LPSPLDVPPMEGTVPRTRQTATRSPADDEPFSALAFKIMSDPYVGRLTYFRVYSGTLR
                     AGAHVLNSTKDRKERVGRILQMHANHREDKEAVFAGDIVAAVGLKHTTTGDTLADPAD
                     PIVLESMSFPAPVISVAIEPKTKADQEKLSQALTRLSEEDPTFMVRFDEETGQTIISG
                     MGELHLEVLVDRLTREWNVGANVGKPQVAYRETIRKPVTRMETRYVKQTGGRGQFGHV
                     VIDLEPTGAGGGYEFVNKVTGGDIPREYIPAVDQGIQEAMESGVLAGYPLVDVRATLT
                     GGSSHDVDSSEMSFKVTGQIALRDSARKADPVLLEPVMQVEVVTPEQFMGDVIGDLTA
                     RRGRIERMDQRGGSQTIRAHVPLAEMFGYATDLRSKTQGRATYTMQFHSYQEVPANIA
                     REIVAKIRGE"
     gene            complement(4203..4673)
                     /gene="rpsG"
                     /locus_tag="VG709_07970"
     CDS             complement(4203..4673)
                     /gene="rpsG"
                     /locus_tag="VG709_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWC33047.1"
                     /translation="MPRRGTRTKREIEPDPIYQNALVIQLINKLMQDGKKTLAERLVY
                     DALERISERTANDPVITLKRAVENARPLLEVRSRRVGGATYQVPVEVKPARGTTLAMR
                     WLVNYARQRRENTMADRLVGEIMDASTGQGNAVKRKEDLHKMAEANKAFAHYRW"
BASE COUNT          618 a         1627 c         1630 g          861 t
ORIGIN      
        1 gccgatcacc accgagcctt cgccgaggcg ctgcgcatcg ccctatcaca ggtgccgggg
       61 ctgcgcgtga cggccgtgac gcacgacgtc gacgagctcg cggaggcggt tcgcgcgcgg
      121 gccgccgacg tcgtcctcgt cgatgagggc ctccccgcgg acggcctgct gtgggcgcta
      181 aaggaaggtg ggagcggtgt ccgggtcctc gtcctcaccg ggagcgacga cgaccagacg
      241 gtctcgaagg ccgcggagcg tggcggcgac ggcgtactcg cgcgcacgcg gccgctgcgc
      301 gagctcgtcc acgcgatcga gtcactctcg cgggggaagt cactcatcga ccaagacgtg
      361 ctgcgacgtt ccacgctccg gcgccgggca cggcgtgagg cggaagccgc gctcgccgag
      421 cgcctgagac gcctgacgag gcgcgaggta gagatcctcc agcagatggc cgacggtctc
      481 ccgccggacg aggtcgccga gcggctcggc atctcgccca acaccctgcg gacccacgtg
      541 caaaacgtgc tcttgaagct ccgtttgcac tcaaagcttc aggcggtgac ggcggcgatc
      601 cgccacggcg tcgtccacgt gcggcgcgac gcgaccgtcg acgtcgagcg gcgagtgctc
      661 gacctgacgg ctagcgggtc tcgcggtgaa gcgtatgccg ccggcaccag cggcagtact
      721 tccggagccc gatccggtcg cggtcgttca cgcggttctt ctcggtgagg tagttccgcc
      781 gcttgcactc ctcgcacgcg agggtgatct tcggccggtt ctcggacttc ggcatctact
      841 tctggatgtt cgtgacgcgg ccggcgccga cggtgcgccc gccctcgcgg atcgcgaagc
      901 gcaggccctc ctccatcgcg atgggggcga tcatcgagac ctgcatcgtg acgttgtcgc
      961 ccggcatcac catctcgacg ccgtcgggga gctggatcgc gccggtgacg tcggtcgtgc
     1021 ggaagtagaa ctggggccgg tagccgttga gaaacggcgt gtggcggccg ccctcgtcct
     1081 tcgagagcac gtagacctgg gcctcgaagt ccgtgtgggg cgtgatggag cccggcgcgg
     1141 ccagcacctg gccgcgggcc acgtcgtcct tgtcgatccc gcgcagcagc acgccgacgt
     1201 tgtcgccggc ctggacctcg tcgaggagct tgcggaacat ctcgaggtcg gtcgcgacgg
     1261 tcttcttggt gtcgtggatc cccacgatcg cgacctcgcc catcttcttg agcgtccccc
     1321 gctcgacgcg gccggtcacc acggtgccgc ggccggtgat cgagaacacg tcctcgatcg
     1381 gcatgaggaa ggggcggtcg acgtcgcggg tgggctcggg gatgtaggtg tcgcaggcct
     1441 ccatgagctc caggatctgg ccgcgggcct cctcgtctcc ctcgagggcc ttgagcgccg
     1501 agacccgcac caccggggtg tcgtcgccgg ggaactcgta ctggttgagg agctcgcgca
     1561 cctcgagctc gacgagatcg aggagctcgg ggtcgtcgac ggcgtcgacc ttgttgaggg
     1621 ccacgacgat cgaggggacg ttcacctggc gggcgagaag gacgtgctcg cgggtcgggg
     1681 gcatggggcc gtcggccgcc gagaccacca ggatggcccc gtccatctgg gcggctcccg
     1741 tgatcatgtt cttgatgtag tcggcgtggc cggggcagtc cacgtgggcg tagtggcgcc
     1801 gctcggtctc gtactcgacg tgggcgatcg cgatcgtgat gccgcgctcc ttctcctcgg
     1861 gcgccttgtc gatctgctcg aacggcgtgt agtcggccat gccccgctcg gcgagcacct
     1921 tggtgatggc ggccgtgagg gtcgtcttgc cgtggtcgat gtggccgatc gtcccgatgt
     1981 tgacgtgcgg cttcgtgcgc tcgaacttct tcttgctcat cgtctcccct ctcgcctact
     2041 cgccccggat cttcgcgacg atctcgcgcg cgatgttcgc cgggacctcc tggtacgagt
     2101 ggaactgcat cgtgtacgtc gcgcgtccct gcgtcttgga ccgcaggtcg gtcgcgtacc
     2161 cgaacatctc cgccagcggc acgtgggcgc ggatcgtctg gctcccgccg cgctggtcca
     2221 tccgctcgat gcgcccacga cgcgcggtga ggtcgccgat cacgtcaccc atgaactgct
     2281 ccggcgtcac gacctcgacc tgcatgaccg gctcgaggag cacgggatcg gccttgcgcg
     2341 ccgagtcgcg cagcgcgatc tggccggtca ccttgaacga catctccgac gagtcgacgt
     2401 cgtgggagct gccgccggtg agcgtcgcgc gcacgtcgac gagcgggtag ccggcgagga
     2461 cgccgctctc catcgcctcc tggatgccct ggtccaccgc cgggatgtac tcgcgcggga
     2521 tgtcgccgcc ggtgaccttg ttgacgaact cgtagccgcc gccggctcca gtcggctcga
     2581 ggtcgatcac gacgtggccg aattgcccgc ggccaccggt ctgcttgaca taccgcgtct
     2641 ccatgcgcgt cacgggcttg cggatcgtct cgcggtacgc gacctgcggc ttgccgacgt
     2701 tcgctccgac gttccactcc cgcgtcagcc ggtcgacgag cacctcgagg tggagctccc
     2761 ccatcccgga gatgatcgtc tgccccgtct cctcgtcgaa ccggaccatg aacgtcgggt
     2821 cctcctccga gagacgcgtg agcgcctggc tgagcttctc ctggtcggcc ttggtcttcg
     2881 gctcgatcgc gaccgagatg accggcgccg ggaacgacat cgactcgagg acgatcgggt
     2941 ccgcggggtc ggcgagcgtg tcgccggtcg tcgtgtgctt cagcccgacg gcggcgacga
     3001 tgtcgccggc gaagacggcc tccttgtcct cgcggtggtt ggcgtgcatc tggaggatcc
     3061 gcccgacgcg ctccttccgg tccttcgtcg agttgaggac gtgcgcgccg gcgcgaagcg
     3121 tcccggagta cacgcggaag taggtcaggc gcccgacgta ggggtccgac atgatcttga
     3181 acgcaagcgc ggagaacggc tcgtcgtcgg ccgggctccg cgtggccgtc tgccgggtcc
     3241 gcggaaccgt cccctccatc gggggaacgt cgaggggcga cggaaggtag tcgacgacgg
     3301 cgtcgaggag cggctggatg cccttgttct tgaacgcggt gccgcacaac acgggcgtcc
     3361 cctcgccggc gagcgtcgcg tcgcggagcg cctggtggag ctcctccggc ggcggctcct
     3421 cgcccgcgac gtacttctcc atgaggccct cgtggtggtc ggccagctgc tcgtagagcc
     3481 ggtggcgcca ctcgagggcg gtctcgcgca ggtcgtccgg gatctcgacg gtctcccact
     3541 cctcgccctt ctcggagctc caccgcaggc ccttcatcgc gacgaggtcg aggacgcccg
     3601 tgaaggactc ctcgataccc cacggcagct ggacgacgag cggcatcgca ttgagccggt
     3661 cctcgatcat gccgacggtg cgctcgaagt cggcgcccac tcggtccatc ttgttgacga
     3721 agcagatgcg cggtacgcgg tagcggtcgg cctgccgcca caccgtctcg gactgtggct
     3781 cgacgcccgc gaccgcgtcg aagatcgcga cggcgccgtc gaggacgcgc agcgaccgct
     3841 ccacctcgac cgtgaagtcg acgtgacccg gcgtgtcgat gatgttgatc cagtggttct
     3901 tccagtggca ggtcgtcgcg gccgacgtga tcgtgatgcc gcgctcctgc tcctggacca
     3961 tccagtccat gacggcggtg ccttcgtgga cctcgccgac cttgtaggtc ttcccggtgt
     4021 aatagaggat gcgctcgggc gtggtcgtct tccccgcgtc gatgtgcgcc atgatcccga
     4081 tgttccgggt gcgctgaagc gggtactcgc ggatgactag gccgcccttg cggcctccgg
     4141 ttctgacctg ctgcgccgtc tctgccacct gcatcttcct cgccggcgtc gccactacgg
     4201 ggtcaccacc ggtagtgggc gaacgccttg ttcgcttccg ccatcttgtg gagatcttcc
     4261 ttccgcttga ccgcgttccc ctgtccggtc gacgcgtcca tgatctcgcc cacgaggcgg
     4321 tcggccatgg tgttctcgcg tcgctgcctc gcgtagttca cgagccagcg catcgcgagc
     4381 gtcgtcccgc gggccggctt cacctcgacc gggacctggt acgtcgcgcc gccgacgcgc
     4441 cgcgaacgga cctcgaggag cggtcgcgcg ttctcgaccg ccctcttgag cgtgatcacg
     4501 gggtcgttcg cggtcctctc gctgatccgc tcgagcgcgt cgtagacgag ccgctcggcc
     4561 agcgtcttct tgccgtcctg catcagcttg ttgatcagct ggatgacgag cgcgttctgg
     4621 tagatcgggt ccggctcgat ctcgcgcttg gtacgggtgc ctcgcctggg catgctcgcg
     4681 tcctaaccgg tcttcttggc gccgtacttg gagcgcgcct tcttgcggtc gccgac
//
LOCUS       DASZOB010000510         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48152, whole genome shotgun sequence.
ACCESSION   DASZOB010000510 DASZOB010000000
VERSION     DASZOB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48152"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1509
                     /locus_tag="VG709_07975"
     CDS             <1..>1509
                     /locus_tag="VG709_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC33048.1"
                     /translation="VEDRPDPQDASERAEQLAREYRATVENIVESRGIPEVADFLRGV
                     DAPGALADLAGYSPDLSFEQKVEVLETLDVEQRLEKVLAWGKDTLAEVSLKDKIRTEV
                     AEGLEKSQREFILRQQLDAIRKELGEGDDEDVAEEYRRKIDDAGMPDGVLEQANRELG
                     RLERTSPQNPEHGWIRTYLDWLVDVPWSKRTEDNLEIDDARRILDEDHTGLDDVKDRI
                     VEYLSVRKLRAERGLAEVTSGRGSGAILTLIGPPGVGKTSLGESVARALGRKFVRVSL
                     GGIHDEAEIRGHRRTYVGALPGRVVRALKDAGTKNPVMMLDEIDKVGADWRGDPSSAL
                     LEVLDPAQNHTFRDHYLDVDLDLSEVLFIATGNVAETIPGPLLDRMEVLRLDGYTADE
                     KVAIARDHLLDRQLERNGLEPHEVEITDEALHVIVDDYTREAGVRNLEREIGKLLRKV
                     ATRVASRSIDTPVRVDAEDIRAYLGRQRFFFEAADRTSVPGVATGLAVTGTGG"
BASE COUNT          267 a          501 c          539 g          202 t
ORIGIN      
        1 gtcgaggacc gtcccgatcc gcaggacgcg tcggagcgcg ccgagcagct cgcccgggag
       61 taccgcgcga cggtcgagaa catcgtcgaa tcgcgaggga tccccgaggt cgcggacttc
      121 ctgcgcggtg tcgacgcgcc tggcgcgctg gccgacctcg cgggttactc gccggatctg
      181 tcgttcgaac agaaggtcga ggtcctcgag acgctcgacg tcgagcagcg gctcgagaag
      241 gtgctcgcgt gggggaaaga caccctcgcc gaggtcagcc tcaaggacaa gatccgcacg
      301 gaggtcgccg agggtctcga gaagagtcag cgcgagttca tcctgcgcca gcagctcgac
      361 gcgatccgca aggagctcgg tgagggcgat gacgaggacg tcgcggagga gtaccgccgc
      421 aagatcgatg acgccgggat gccggatggc gttctcgagc aggcgaaccg cgagctcggc
      481 cggctcgagc gcacgtcgcc gcagaacccc gagcatggat ggatccgcac gtacctcgac
      541 tggctcgtcg acgttccgtg gtcgaagcgc accgaggaca acctcgagat cgacgacgca
      601 cgccggatcc tcgacgagga ccacacgggt ctcgacgacg tgaaggaccg catcgtcgag
      661 tacctgtccg tgcggaagct ccgcgccgag cgaggcctcg ccgaggtgac gagcgggcgc
      721 ggctccggcg cgatcctcac gctgatcgga ccgccgggcg tcggcaagac gagcctggga
      781 gagtcggtcg cgagagcgct cgggaggaag ttcgtccggg tctcgctggg cggcatccac
      841 gacgaggcgg agatccgcgg ccaccgccga acgtacgtcg gcgcgctccc gggacgcgtc
      901 gtccgcgcgc tcaaggacgc cggcaccaag aaccccgtga tgatgctcga cgagatcgac
      961 aaggtcggcg ccgactggcg aggcgacccg tcgagcgcgc tgctcgaggt gctcgatccc
     1021 gcgcagaacc acacgttccg cgaccactac ctcgatgtgg acctcgatct gtcggaggtg
     1081 ctgttcatcg cgacggggaa cgtcgccgag acgatccccg gtcctctgct ggaccggatg
     1141 gaggtcctcc ggctcgacgg gtacaccgcg gacgagaagg tcgcgatcgc gcgcgaccac
     1201 ctgctcgacc ggcagctcga gcgcaacggg ctggagccgc acgaggtcga gatcaccgac
     1261 gaggcgctgc acgtgatcgt cgacgactac acgcgcgagg cgggcgtccg taacctcgag
     1321 cgcgagatcg gcaagctgct ccgcaaggtc gccacgaggg ttgcttcgag gagcatcgac
     1381 acgcccgttc gagtcgatgc ggaagacatc cgtgcctacc tcggccgaca gcggttcttc
     1441 ttcgaggctg cggaccgcac cagtgtgccc ggcgtcgcga cgggcctcgc cgtgaccggc
     1501 accgggggc
//
LOCUS       DASZOB010000511         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_179707, whole genome shotgun sequence.
ACCESSION   DASZOB010000511 DASZOB010000000
VERSION     DASZOB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179707"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VG709_07980"
     CDS             complement(<1..247)
                     /locus_tag="VG709_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWC33049.1"
                     /translation="MFLRENEIGRKVAPHLERGTTLLDLGAGTGRIARWLAERTGVRP
                     TLADVGEFGNRDRTFPFIRMTDPLRVPADDASFDAVLM"
     gene            396..785
                     /locus_tag="VG709_07985"
     CDS             396..785
                     /locus_tag="VG709_07985"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWC33050.1"
                     /translation="MRIRSHDQGFVAAPPTDVYRTVADLGRYPSWWDAVHVDPGDPAT
                     IALEPRVTAPVTRRQEREGTGLFLELGPPYDGTLEWYLEPFEDGTIVNCLLDIELAGS
                     RRRVRLRLWRLRARVRAGLVGIKRALE"
     gene            796..1500
                     /locus_tag="VG709_07990"
     CDS             796..1500
                     /locus_tag="VG709_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWC33051.1"
                     /translation="MTERRCPRCGGLVGEDARWCGQCLARLDVDEPRSSATESGTERP
                     PRREASDRPLPPQGGPIEVEDGRALWSCPSCGARNPLDDDVCPRCGRTFASLFQEPAP
                     PPHIEARRAAALSLLAPGVGHFAAGRIAEGIARLIVFAYALGTALVVRLATGIRSAGP
                     LVPLGWLSLAAAVALYVTSAVDAARVADRRDPVLSNRALLYGAIGLMMLTVIVLVVSG
                     LRVTSVRDGGTAAPAW"
     gene            1510..>2270
                     /locus_tag="VG709_07995"
     CDS             1510..>2270
                     /locus_tag="VG709_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWC33052.1"
                     /translation="MRLHVISDLHGAVERLRDAGKGCDAVLVLGDLINVLDYHSMEGI
                     LVDVFGREPVAEAAGLRARGRYVEARRAMRRRLGDEEELRIRFVELAREEYERVFDAL
                     PDNSFVTYGNVDIPDLLRSFVRPGVRFVDGEVVELGGERFGIVGGGLRTPLGIPGEVD
                     EDEYDRKLAALGPVEVVCTHQPPRIPWLVYDVLGRRFEPGSTGLVGYIQEHQPRRSYF
                     GHVHQPLSSRGRIGRTELVNVGYFRGSGEPFVPDTT"
BASE COUNT          294 a          769 c          855 g          352 t
ORIGIN      
        1 gcatgaggac cgcgtcgaag gaggcgtcgt cggccgggac ccgcaacggg tccgtcatgc
       61 ggatgaacgg gaacgtgcgg tcgcgattgc cgaactcgcc gacgtcggcg agcgttgggc
      121 ggacgccggt ccgctcggcg agccatcgcg cgatccgccc ggtgcccgcc ccgaggtcga
      181 gaagcgtcgt cccacgctcg aggtgcggcg cgaccttccg cccgatctcg ttctcgcgca
      241 ggaacacggg accagcgtag gcgcatgcga gaatcgcgac ccgtggagtg gagcggtcgc
      301 cgtgacgccg gggcgagcgt cgcgcaggtc gtcttggcgc tgttcgtgct cgcggtcacc
      361 gcgctggtcg tcgtcatcgc gctccggcgc gtgctgtgag gattcgctcc cacgatcagg
      421 gcttcgtcgc ggcaccgccc accgacgtgt accgaaccgt cgcggacctc ggccgatatc
      481 cgtcctggtg ggacgcggtc cacgtcgacc ccggagaccc cgcgacgatc gccctcgagc
      541 cgcgcgtgac cgctcctgtc acgaggcggc aagagcgcga aggcaccggg ctcttcctcg
      601 agctcgggcc gccgtacgac ggcacgctcg agtggtatct ggagccgttc gaggacggca
      661 cgatcgtgaa ctgcctgctc gacatcgagc tggcggggag ccggcggcgc gtgcgcctaa
      721 ggctctggcg gctccgggca cgggtgcgcg ccgggctggt cgggatcaag agggccctcg
      781 agtgaacggt cgcgtatgac cgagcgcagg tgccctcggt gcggagggct ggtcggcgag
      841 gacgcgcggt ggtgcggtca gtgcctcgcg cgtctggacg tcgatgaacc gcggtcgagc
      901 gcaacggaat ccggcaccga gcggccgccg cggcgcgagg cctccgaccg gccgctcccg
      961 ccgcagggcg ggccgatcga ggtcgaggac ggtcgcgcac tgtggtcgtg tccgtcgtgc
     1021 ggcgcgcgga atccgttgga tgacgacgtg tgtccgcgct gcgggcgtac gttcgcgtcg
     1081 ctcttccagg agccggcgcc gccgccgcac atcgaggcac gacgcgcggc ggcgctgtcg
     1141 ctgctcgcgc cgggcgtcgg gcatttcgcg gcgggtcgga tcgccgaggg gatcgcgcgc
     1201 ctcatcgtgt tcgcctacgc gctggggacg gcgctcgtcg tgcgccttgc aaccggcatc
     1261 cgcagcgccg gtccgctcgt gccgctcggc tggctgtccc tcgctgcggc cgtcgcgctc
     1321 tacgtgacgt cggcggtcga tgcggcacgc gtcgccgacc ggcgtgaccc ggtcctctcc
     1381 aaccgagcgc tgctctacgg ggcgatcggc ctcatgatgc tcacagtcat cgtcctcgtc
     1441 gtgagcgggc tccgggtcac gtccgtgcga gacggaggca cggcagcgcc tgcgtggtaa
     1501 gaaacgcgcg tgcggctcca tgtgatctcg gacctccacg gcgccgtgga gcgcctacgc
     1561 gacgcgggga agggctgcga cgcggtgctc gtgctcggcg acctcatcaa cgtcctcgac
     1621 tatcactcga tggaaggcat cctcgtcgac gtgttcggcc gtgagcctgt ggccgaggcg
     1681 gccgggctgc gggcccgcgg gcggtacgtc gaggcgcgac gggcgatgcg gcggcggctc
     1741 ggggacgagg aggagctgcg gatccgcttc gtcgagctcg cacgagagga gtacgaacgc
     1801 gtcttcgatg cgctccccga caattcgttc gtgacctacg gcaacgtcga catccccgac
     1861 ctgttgcggt cgttcgttcg gccaggcgtg cggttcgtcg acggcgaggt cgtcgagctc
     1921 ggcggcgagc gcttcggcat cgtgggcggc gggctccgga cgccgctcgg gatccccggg
     1981 gaggtcgacg aggacgagta cgaccggaag ctcgcggctc tcggtcccgt cgaagtcgtc
     2041 tgcacgcatc agccgccgag gatcccgtgg ctcgtgtacg acgtcctcgg gcgccgtttc
     2101 gagccgggct cgacggggct cgtcgggtac atacaggagc accagccgag gcgatcgtac
     2161 ttcgggcacg tccatcagcc gctctcgtcg cgggggcgca tcggccgcac ggagctcgtg
     2221 aacgttgggt acttccgcgg gagcggggaa ccattcgtgc ccgacacgac
//
LOCUS       DASZOB010000512         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17654, whole genome shotgun sequence.
ACCESSION   DASZOB010000512 DASZOB010000000
VERSION     DASZOB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17654"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VG709_08000"
     CDS             <1..313
                     /locus_tag="VG709_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWC33053.1"
                     /translation="GAARDELERQLDGLFGQRPAGDWLDLLVAAGIPCGAVNDVGDVM
                     EHPQLAHNRLVAEVGSPAGPLPVVGSPFLVAGQRSELGGVPALGEHTDEVFRERGVEP
                     A"
     gene            complement(242..1132)
                     /locus_tag="VG709_08005"
     CDS             complement(242..1132)
                     /locus_tag="VG709_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWC33054.1"
                     /translation="MSESWTDEQLAAFGRDGFLIVEEGFLDDETVDVLRERFARLFAQ
                     DYETGIRPDEVNWMAGRDPEDRTRQICNGWKADPWIAAQVLSERTGRLAAALLGWDGV
                     RLLQDNCIWKPPGTKSLGMHQDGSYLDYLVPPEMITCWIALDDTSAEAGTITYAAGSH
                     TWPRSPESRGEFHAPPDWLAPLERALPPGEELRLVPIEVKAGGAAFHHFNTFHGSGPN
                     TASVHRRAVISHLVPADAQFHPVHVDPVYSRYRRVGDTTMDESYFPIVWTRDGGRSGW
                     LDAALAEHLVGVLAERGNTT"
     gene            1191..>1735
                     /locus_tag="VG709_08010"
     CDS             1191..>1735
                     /locus_tag="VG709_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWC33055.1"
                     /translation="MAPASLLDAPELAVERIDARAKGENFPVASFLAPRRARPHLRAI
                     YGFARLVDNLGDEADGDREALLDELERELEGPPRSDVMRRVHATIAACGLPREPFARL
                     IEANRIDQRKSRYETWDELRAYCAYSADPVGRLVLGVYGRADDAGLVARSDDVCTGLQ
                     LVNLVQDPPRDLALGRIYLPQ"
BASE COUNT          234 a          629 c          613 g          259 t
ORIGIN      
        1 cggcgccgcc cgcgacgagc tcgagcggca gctcgacggc ctgttcggcc agcgtccagc
       61 cggcgactgg ctcgacctcc tcgtcgcggc cggcatcccg tgcggcgcgg tcaacgacgt
      121 cggagacgtc atggagcacc cacaactcgc gcacaaccgc ctcgtcgcgg aggtcggctc
      181 gcccgccggc ccgcttccgg tcgtgggtag ccccttcctc gtcgccgggc agcgctccga
      241 gctaggtggt gttcccgcgc tcggcgagca caccgacgag gtgttccgcg agcgcggcgt
      301 cgagccagcc tgagcggccc ccgtcgcgcg tccagacgat cgggaagtac gactcgtcca
      361 tcgtcgtgtc gccgactcgg cggtagcgcg agtagacggg atcgacgtgg acggggtgga
      421 actgcgcgtc ggccgggacg aggtgggaaa tgactgcgcg cctgtgcacc gaggcggtgt
      481 tcggtcccga gccgtggaag gtgttgaagt ggtggaaggc ggcgccgccc gctttcacct
      541 cgatcggaac gaggcgaagc tcctcgccgg gcggcagcgc gcgctcgagg ggtgccagcc
      601 agtcgggtgg ggcgtggaac tcgccgcggc tctccggcga gcgcggccac gtgtgcgagc
      661 cggccgcgta cgtgatcgtc cccgcctcgg ccgatgtgtc gtccagggcg atccagcagg
      721 tgatcatctc cggagggacg aggtagtcga ggtagctgcc gtcctggtgc atgccgagcg
      781 acttcgtgcc cggcggcttc cagatgcagt tgtcctgcag gaggcggacg ccgtcccagc
      841 cgaggagcgc ggcggccagg cggccggtgc gctcggacag gacctgcgct gcgatccacg
      901 gatccgcctt ccagccgttg cagatctggc gcgtccggtc ctcggggtcg cgaccggcca
      961 tccagttgac ctcgtccggg cggatgcccg tctcgtagtc ctgcgcgaac agtcgagcga
     1021 accgctcccg gaggacgtcg accgtctcgt cgtcgaggaa cccttcttcg acgatgagaa
     1081 acccgtcgcg gccgaacgcc gcgagctgct cgtcggtcca gctctcgctc acctcgtcgt
     1141 ccgtcgctcc tcgtcgagcc ccgacgcgtc gctccatact atgctcgccg atggctcccg
     1201 cgagcctact cgacgcaccc gagctcgccg tcgagcggat cgacgcccgc gcgaagggcg
     1261 agaacttccc cgtcgcctcg ttcctcgcgc cccgccgcgc gcgccctcac ctgcgtgcga
     1321 tctacggttt tgcccggctc gtcgacaacc tcggtgacga ggccgacggc gaccgcgagg
     1381 cgctcctcga cgagctcgag cgcgagctcg aggggccgcc gcggagcgat gtcatgcgcc
     1441 gcgtgcacgc gacgatcgcg gcgtgcggct tgccgcgcga gccgttcgca cggctaatcg
     1501 aggcgaaccg gatcgaccag cgcaagtcgc ggtacgagac gtgggacgag ctgcgcgcgt
     1561 actgcgcgta ctcggccgac cccgtcgggc gcctcgtgct cggcgtgtac ggccgcgcag
     1621 acgacgccgg gctcgtcgcg cgaagcgacg acgtctgcac ggggctccag ctcgtcaacc
     1681 tcgtccagga cccgccgcgt gacctcgcgc tcgggcgcat ctacctcccc caaga
//
LOCUS       DASZOB010000513         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17795, whole genome shotgun sequence.
ACCESSION   DASZOB010000513 DASZOB010000000
VERSION     DASZOB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17795"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VG709_08015"
     CDS             <1..274
                     /locus_tag="VG709_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWC33056.1"
                     /translation="VLHHHERWDGTGYPDRRIGEEIPLGSRILFVADAYDAMTSERVY
                     SSKLSHERALAELDRCAGTQFDPDVVDAFRSEFAEPSRLELVAESA"
     gene            complement(280..1866)
                     /locus_tag="VG709_08020"
     CDS             complement(280..1866)
                     /locus_tag="VG709_08020"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWC33057.1"
                     /translation="MAKTEEPRRVSEPSVDEKLAELERLREEALHAGDERAVARQRER
                     GKLLARERLEKLLDPGSFVELDRFVRHRNPHFGMMERRPWGDAVVTGYGTIFGRKVFV
                     FSQDFTVFGGTLSEAFAEKICKVMDLALKYGCPVIGINDSGGARIQEGVVSLGGYAEI
                     FWRNVQASGVVPQISLVMGPTAGGAVYSPAMTDFVLMVKETSYMFITGPEVVKTVTGE
                     EVTFEELGGAATHASKSGVAHFAADDEEAALEDARYLFSFLPQNNVDPPPYFAPADPL
                     DREDPELDRIVPDSPNKPYDMRDVIHRVVDEGEFLEVHELWAENLICGFARLAGHAVG
                     VVANNPAALAGTLDIDSSVKGARFVRFCDAFNLPLVTFVDVPGFLPGTAQEWGGIIRH
                     GAKLLYAYCEATVPKIAVITRKAYGGAYDVMSSKHIRADFNVAWPTAAVAVMGPEGAV
                     NIVFRKELEEAEDADARRAELIAEYRARFANPYTAAERGYVDDVIEPRRSRPVLVEAL
                     KAAITKRERRPPRKHGNIPL"
     gene            complement(1917..2294)
                     /locus_tag="VG709_08025"
     CDS             complement(1917..2294)
                     /locus_tag="VG709_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33058.1"
                     /translation="MRINFITPCPVYANGGAHACGPIDWGPAEIGIAVEAFLVQESAY
                     ARGSESFSPSDAMWELHTDPVQGKLKPGLAVGFGVARVTMRSKHEGDVLHTVCWCDVL
                     TLVDEATVRGGQKPKRGGGRAKR"
     gene            complement(2291..>2675)
                     /locus_tag="VG709_08030"
     CDS             complement(2291..>2675)
                     /locus_tag="VG709_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWC33059.1"
                     /translation="GRYDEAERFTEISEAAAANDDVGSQMSWRSARAKVLAKRGTVDE
                     AERLAREAVSLAGRTDWLNEEAKTLVDLGVVLREAERMEEAEAAIRHGIEVYERKGNL
                     AAVDMTRALLGELAPASETLQGGSG"
BASE COUNT          379 a          995 c          866 g          435 t
ORIGIN      
        1 ggtcctgcac caccacgagc gctgggacgg caccggctat cccgaccgtc gcatcggcga
       61 ggagatcccg ctcggctcgc gcatcctctt cgtcgccgac gcctacgacg cgatgacgtc
      121 cgagcgcgtc tacagctcga agctctcgca cgagcgcgcg ctcgccgagc tcgaccgctg
      181 cgccgggacg cagttcgacc ccgacgtggt ggacgcgttc cgctccgagt tcgccgagcc
      241 gagccgcctc gagctcgtcg ccgagtccgc ctagcccgct tagagcggga tgttcccgtg
      301 cttgcgggga ggccggcgct cgcgcttggt aatcgccgcc ttcagggcct cgacgagaac
      361 tggccgtgag cgccggggct cgatgacgtc gtcgacgtag ccgcgctccg ccgcggtgta
      421 cgggttcgcg aaccgcgccc ggtactcggc gatgagctcc gcgcggcgag cgtccgcgtc
      481 ctccgcctcc tcgagctcct tccggaacac gatgttgacg gcgccctcgg ggcccatgac
      541 agccaccgcg gccgtcggcc aggcgacgtt gaagtcggcg cggatgtgct tcgagctcat
      601 gacgtcgtag gcgccgccgt acgccttccg ggtgatgacg gcgatcttcg gcaccgtggc
      661 ctcgcagtag gcgtacagca gcttcgcgcc gtgccgaatg atcccgcccc actcctgcgc
      721 cgtccccggc agaaagcccg gcacgtccac gaacgtgaca agcggaaggt tgaaggcgtc
      781 gcagaagcgg acgaagcgcg cgcccttcac cgacgagtcg atgtccagcg tgcccgcgag
      841 cgccgcgggg ttgttggcga cgacgccaac cgcgtggccg gcgaggcgcg cgaacccgca
      901 gatcaggttc tccgcccaga gctcgtggac ctcgaggaac tcgccctcgt ccacgacccg
      961 gtggatcacg tcgcgcatgt cgtagggctt gttcgggctg tcggggacga tccgatcgag
     1021 ctcggggtcc tcgcggtcga gcgggtccgc gggggcgaag tagggcggcg ggtcgacgtt
     1081 gttctgcggg agaaacgaga acaggtagcg ggcgtcctcg agcgccgcct cctcgtcgtc
     1141 cgcggcgaag tgcgcgacgc cggacttcga cgcgtgcgtc gccgcgccgc cgagctcctc
     1201 gaacgtcacc tcctccccgg tcacggtctt cacgacctcg ggacccgtga tgaacatgta
     1261 ggaggtctcc ttcaccatga ggacgaagtc cgtcatcgcc ggcgagtaga ccgcgccgcc
     1321 cgcggtgggc cccatgacga ggctgatctg tgggacgacg cccgaggcct gcacgtttcg
     1381 ccagaagatc tcggcgtagc cgccgagcga caccaccccc tcctggatcc gtgcgccgcc
     1441 cgagtcgttg atcccgatca ccgggcagcc gtacttgagc gccaggtcca tcaccttgca
     1501 gatcttctcg gcgaacgcct ccgagagcgt gccgccgaag acggtgaagt cctgcgagaa
     1561 gacgaagacc ttgcggccga agatcgtccc gtaccccgtc acgaccgcgt cgccccacgg
     1621 acgccgctcc atcatcccga agtgcgggtt ccggtggcgg acgaagcggt cgagctcgac
     1681 gaagctgccc gggtcgagga gcttctcgag ccgctcgcgc gcgagcagct tcccgcgctc
     1741 ccgctgccgc gctaccgccc gctcatcgcc ggcgtggagc gcctcctcgc ggaggcgctc
     1801 cagctcggcc agcttctcgt cgacgctcgg ctcgctcacc cgccgcggct cctccgtctt
     1861 cgccacggcg agagattacg gcacgctagg cggaggcgat ccgggccggc ggcggctcag
     1921 cgcttcgcgc ggcctcctcc acgcttcggc ttctggccac cgcgcactgt tgcctcgtcc
     1981 acgagcgtca gcacgtcgca ccagcagacg gtgtgcaaga cgtcgccctc gtgcttgctc
     2041 cgcatcgtca cgcgcgcgac gccgaagccc acggcgagcc ccggcttgag cttcccctgc
     2101 accgggtcgg tgtgcagctc ccacatcgcg tcgctcgggc tgaagctctc gctccctcta
     2161 gcgtatgcgc tctcctggac gaggaaggct tcgaccgcga taccgatctc cgccgggccc
     2221 caatcgatcg ggccgcacgc atgcgcgcca ccgttcgcat agacggggca cggcgtgatg
     2281 aagttgatcc tcatcctgaa cctccttgca gggtttccga cgcgggcgcg agctccccca
     2341 ggagcgcacg cgtcatgtcc acggcggcga ggttgccctt ccgctcgtac acctcgatcc
     2401 catgacggat cgcggcctcc gcttcttcca ttctctcggc ctcgcgaagc accacgccga
     2461 gatcgacgag cgtcttcgcc tcctcgttga gccaatccgt tctccccgcg agtgagaccg
     2521 cctcgcgcgc gaggcgctcg gcctcgtcaa ccgtccctcg cttcgcgagg accttggcac
     2581 gcgcgcttcg ccagctcatc tgcgagccga catcgtcgtt tgcggcggcg gcctcgctga
     2641 tctcggtgaa gcgctcggcc tcgtcgtacc ttccc
//
LOCUS       DASZOB010000514         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17817, whole genome shotgun sequence.
ACCESSION   DASZOB010000514 DASZOB010000000
VERSION     DASZOB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17817"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VG709_08035"
     CDS             <1..1385
                     /locus_tag="VG709_08035"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWC33060.1"
                     /translation="IQMKAELGRYRMRGFSMFKKIPHWDDLMFLPGTLTRFVIEGYQE
                     KCETKTVLGARFAQKPLELDIPIYITGMSVGALSLEAKMALAKGASMAGTATCSGEGG
                     MTPPERDLSTKWYYQLIQSRYGFNPHHLMLADACEFFIGQGCKVGLGGHLMGQKVTEQ
                     VAEMRSLPPGIDQRSPARHPDWLGPDDLSLKVQEVREATDYQIPIQLKLGAARVYDDV
                     RMAAKCGPDIIYLDGAEGSTGAGPHTATEETGIPLIAAIPEARRALEDVGLADEIDLV
                     VAGGIRNGADVAKCLSLGAKAVAIGHSALIALNCNKEIPGVTDYEGTVGVPAGECYHC
                     HTGRCPVGVTTQDPELRKRLQVDEAAERVYNFLHAMTLELQMLARACGKTDVHSLEPE
                     DLCALTVEAAAMAKVPLAGTHYIPGVTEERTLAEIKQLLERHLEYPVDYLPSEATPSA
                     SAYIASGGGE"
     gene            1391..1882
                     /locus_tag="VG709_08040"
     CDS             1391..1882
                     /locus_tag="VG709_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33061.1"
                     /translation="MPDEPQAGSVAEAQEGHPSATSAREPEKKVAIDAEFLAGHGFPY
                     QRDIALVEDVDLLAATPGDDLNWLEDVELLQEDGVPAVFDRYSNSFLKIYFEIPEGRE
                     NEIARKVLVKHLQSGNSYGIHLKEQHAKFPQPELGPWVEDSATVGTNWKPSKLEGWEP
                     PLH"
     gene            1931..>2121
                     /locus_tag="VG709_08045"
     CDS             1931..>2121
                     /locus_tag="VG709_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HWC33062.1"
                     /translation="MLALGFALGAALAWGTADFLGGLTTRGLGVLTVLLVSQAFGAGL
                     LAVVIAATAQPVPDTRRLV"
BASE COUNT          352 a          751 c          724 g          294 t
ORIGIN      
        1 acatccagat gaaggccgag ctcggccgtt accgcatgcg cgggttctcg atgttcaaga
       61 agatcccgca ttgggacgac ctcatgttcc tccccgggac gcttaccagg ttcgtgatcg
      121 aggggtacca ggagaagtgc gagacgaaga ccgtgctggg cgcccgcttc gcgcagaagc
      181 cgctcgagct cgacatcccg atctacatca cgggcatgag cgtcggcgcc ctctcgctcg
      241 aggcgaagat ggcgctcgcg aagggcgcgt cgatggccgg gacggcgacg tgctccgggg
      301 aaggcggcat gacgccgccg gagcgcgacc tctcgaccaa gtggtactac cagctcatcc
      361 agagcaggta cgggttcaac ccgcaccacc tcatgctggc cgacgcctgc gagttcttca
      421 tcggacaggg ctgcaaggtc ggcctcggcg gtcatctcat gggccagaag gtgaccgagc
      481 aggttgccga gatgcgctcg cttccgcccg ggatcgacca gcgctcgccc gcccgccacc
      541 ccgactggct cgggccggac gacctctcgc tcaaggtgca ggaggtccgc gaggcgaccg
      601 actaccagat cccgatccag ctgaagctcg gcgcggcgcg cgtctacgac gacgtccgca
      661 tggccgcgaa gtgcgggccc gacatcatct atctcgacgg cgccgagggc tcgacgggcg
      721 ccgggccgca caccgcaacg gaggagacgg ggattccgct catagcggcc attcccgagg
      781 cccggcgggc gctcgaggac gtcgggctcg cggacgagat cgacctcgtc gtcgccggcg
      841 gcatcaggaa cggcgccgac gtcgcgaagt gcctctcgct cggcgcgaag gccgtcgcga
      901 tcgggcactc ggcgctgatc gcgctcaact gcaacaagga gattccgggc gtcacggact
      961 acgagggaac ggtgggcgtc cccgccggcg agtgctacca ctgccacacc ggccgttgcc
     1021 ccgtgggcgt gacgacgcag gaccccgagc tccgcaagcg cctccaggtc gacgaggcgg
     1081 ccgagcgcgt ctacaacttc ctccacgcga tgacgctcga gctccagatg ctcgcgcgcg
     1141 cgtgcgggaa gaccgacgtg cactccctgg agccggagga cctgtgcgcg ctcaccgtcg
     1201 aggcggcagc gatggcgaag gtgccgctcg ccggcacgca ctacattccg ggcgttaccg
     1261 aggagcgcac gctcgccgag atcaagcagc tcctcgagcg ccacctcgag tacccggtcg
     1321 actacctccc gagcgaggcg acgccgagcg cgagcgcgta catcgcgagc ggcggcgggg
     1381 agtaggcgcg atgccggacg agccgcaagc cgggtcggtc gccgaggcgc aggaggggca
     1441 tccctccgcg acgtcggcgc gcgagcccga gaagaaggtc gcgatcgacg ccgagttcct
     1501 cgcggggcac ggattcccgt accagcgcga catcgcgctc gtcgaggacg tcgacctcct
     1561 cgcggccacg cccggtgacg acctcaactg gctcgaggac gtcgagctcc tgcaggagga
     1621 cggcgtcccg gccgtcttcg accgctactc caactcgttc ctcaagatct acttcgagat
     1681 tcccgaaggg cgcgagaacg agatcgcgcg caaggtgctc gtgaagcacc tccagtccgg
     1741 caactcgtac gggatccacc taaaagagca gcacgcgaag ttcccgcagc cggagctcgg
     1801 cccctgggtg gaggattcgg cgacggtcgg aaccaactgg aagccttcga agctcgaggg
     1861 ctgggagccg ccgctccact agctcgcgtc gccgcccgcg cggacccgct cggagcgacg
     1921 gggctctcgg atgctcgccc tcggattcgc cctcggagct gcgctcgcgt ggggaacggc
     1981 cgacttcctc ggcggcctga cgacgcgcgg cctcggggtc ctcacggtcc tcctcgtctc
     2041 ccaggcgttc ggcgccggcc tcctcgccgt cgtgatagcg gcgaccgcgc agcccgtccc
     2101 ggacacgcgc cggctcgtct t
//
LOCUS       DASZOB010000515         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_38041, whole genome shotgun sequence.
ACCESSION   DASZOB010000515 DASZOB010000000
VERSION     DASZOB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38041"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VG709_08050"
     CDS             complement(<1..845)
                     /locus_tag="VG709_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33063.1"
                     /translation="MTAEPKSFEGSADRSILLIGEGKLADATQRAIQAAGGSVARLPD
                     PSDADIRQALEEQVDSVLIVSRDDHVSLRFALVIETERPGMALIATVHGRIIAAQLER
                     VVENARVISRADIVAPVLAAACLDERLLLVQQTDAGWRGVHADDGTPALRSVEPARQG
                     LWQRLLANVGSLVNPFEVSGRILMAGLAGFLVVLAVDALATALALDQSPIDALYAATK
                     AIVTGGPNPKVETGPAWFKVFSAAAMVAALALTAIFTAGVVDRLLSRRLTAMLGPRSV
                     PRKDHV"
     gene            1147..>1650
                     /locus_tag="VG709_08055"
     CDS             1147..>1650
                     /locus_tag="VG709_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWC33064.1"
                     /translation="MPDDRLKALEERTRYSPAEVEERVFARWEEAGIFHPEPEGDASE
                     NFSIAVPPPNVTGALHLGHALNGSIQDVLVRTARMRGLRAKWIYGTDHAGIATQVKVE
                     EALREEGKTKEDLGREAFVERVWEWRREHGSTITEQFKRLGASLDYADERFTMDDGYA
                     LAVAHVFV"
BASE COUNT          260 a          571 c          594 g          225 t
ORIGIN      
        1 acgtggtcct tgcgcgggac agagcgcggg ccgagcatcg cggtcagccg gcgcgagagc
       61 aggcggtcga cgacgccggc ggtgaagatc gcggtcagcg cgagcgccgc gaccatcgcc
      121 gccgcggaga agaccttgaa ccaggcgggt ccagtctcga ccttggggtt cggaccgccc
      181 gtgacgatcg ccttcgtcgc cgcgtagagc gcgtcgatcg gcgactggtc aagcgcgagc
      241 gcggtcgcca gcgcgtcgac cgcgagcacg acgaggaacc cggcgaggcc cgccatcagg
      301 atccgaccgc tcacctcgaa cgggttcacg agcgagccga cgttcgcgag cagccgctgc
      361 cagagaccct gacgcgcggg ctcgaccgag cgcagcgctg gcgtgccgtc gtccgcgtga
      421 accccacgcc agcccgcgtc tgtctgctgg acgagcagca gccgctcgtc aagacaggcg
      481 gccgcgagca cgggggcgac gatgtcggcg cgcgatatca cgcgcgcgtt ctcgacgacg
      541 cgctcgagct gtgcggcgat gatccgtccg tggacggtcg cgatcagcgc catcccgggg
      601 cgctcggtct cgatcacgag cgcaaagcgc agcgagacgt gatcgtcgcg ggagacgatc
      661 agcaccgagt cgacctgctc ctcgagcgcc tggcggatgt ccgcgtcgct tgggtcgggc
      721 aggcgggcga ccgaccctcc ggcggcttgg atcgcccgct gggtcgcgtc ggccaacttg
      781 ccctctccga tcaggaggat cgaccgatcg gcggagccct cgaaggattt cggttctgcg
      841 gtcacaggcg cgattcgcaa agggcgtcgc gaagcgaggc ctcgcctggc gacattcgac
      901 cccgctgcgc ggaggccttc ccacgcgcgc ccgccgacgc gagctgcctc gacctcacga
      961 ccggcatcga cgctctactg gcggcggggg gcgctttgcc gccgcaggac cctagatcct
     1021 gaacctcgct actcgcgcgt acatgacatc ccttctctcg ccggcttgga cggccgagcc
     1081 tatccgcgcg gaagcaggcg ccggctcggc ccactcatgg agatctcggg ctggcagact
     1141 ggatggatgc cggacgaccg tctgaaggcc ctcgaggaac gcacgcgcta ctcgcccgcg
     1201 gaggtcgagg agcgcgtctt cgcgcgctgg gaggaggccg ggatcttcca tcccgagccc
     1261 gagggcgacg cgagcgagaa cttctcgatc gccgtgccgc cgccgaacgt caccggcgcg
     1321 ctccatctcg gccacgccct gaacggctcg atccaggacg tgctcgtgcg cacggcccgc
     1381 atgcgggggc tgcgcgcaaa gtggatctac ggcaccgacc acgccgggat agccactcag
     1441 gtgaaggtcg aggaggcgct gcgggaggag ggcaagacga aggaggacct cggtcgtgag
     1501 gcgttcgtgg agcgcgtctg ggagtggcgg cgcgagcacg gctcgacgat caccgagcag
     1561 ttcaagcgcc tcggggcgtc gctcgactac gccgacgagc gcttcacgat ggacgacggc
     1621 tacgcgctcg cggtcgcgca cgtgttcgtc
//
LOCUS       DASZOB010000516         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_180021, whole genome shotgun sequence.
ACCESSION   DASZOB010000516 DASZOB010000000
VERSION     DASZOB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180021"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..921
                     /locus_tag="VG709_08060"
     CDS             202..921
                     /locus_tag="VG709_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HWC33065.1"
                     /translation="MDRTDQQLARLSPLAEPLRRAVYLYVAAKRTDVSRDKVASALGI
                     GRSLAAFHLDKLLEHGLVEARYRRLSGRTGPGAGRPSKLYRATEEIRVTVPSRDYELA
                     ARLLVEASRRDRRLETVEAVAFEAGRAAGSAAGRRDVSAAAVRRRLRRLLAERGYEPH
                     VARGDIRLRNCPFHALAEEYRDTICPMNRALITGMLDGLGAQDLEAVPQQVSGECCVA
                     IRATGSSTRSEVRHRRKTSLN"
     gene            1238..1549
                     /locus_tag="VG709_08065"
     CDS             1238..1549
                     /locus_tag="VG709_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33066.1"
                     /translation="MLAQISLSDNPCCQFINDVPQWFFLLVHVAIFVVAAVLAVRAFG
                     VDARGVGWGFALFALAEVSYLTYHLNVTLFLLAHTVSEVFVLIGILLIGAGVVRKGTA
                     R"
     gene            1607..1930
                     /locus_tag="VG709_08070"
     CDS             1607..1930
                     /locus_tag="VG709_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWC33067.1"
                     /translation="MLAVGLVACTSGGSGPVADEQPVRTDTVDLPKSYRFEPAVIEVD
                     AGTTVTWTNNDDFPHNVHLLGGRDVTEPLPVGGTATITFDEPGEVPYECSLHPQQMKG
                     TVIVT"
     gene            1970..>2534
                     /locus_tag="VG709_08075"
     CDS             1970..>2534
                     /locus_tag="VG709_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33068.1"
                     /translation="MAGNATTGAARLSGRDVAKFTALVALLAIFSDAALGHGLTWEND
                     PYWTYWVTKTFLIATVFGLGTAWVGIGAGRGAAITLVHTVVLTVYYWTLSPIGLPSSP
                     VWLDLEHTWITGVPIHFAVIYLGYLAALWLWRRRGAADVEERTDRAPFGTSALVVGLA
                     ITIAAGGIASLALTDFPGVTWFLTRVLI"
BASE COUNT          354 a          861 c          909 g          410 t
ORIGIN      
        1 gcggctctgg accgcgtcgc gatcggcggt ggggcgaggg gcgtgaacct ccacctcgcg
       61 cccgccgacc tcctcgcgca tctcggggcc gacgtcgcgg acgtgacgcg tccggcctga
      121 accgcccgcc ctgaaccggc ggcgtttcgc caatcgccgc tgtggaaaga aaacgtcgac
      181 cggcggtgcc tgtgatggag gatggacagg acggaccagc agctcgcgag gctctcgccg
      241 ctcgccgagc cgctcaggcg agcggtatat ctgtacgtcg ccgccaagcg gacggacgtg
      301 agccgcgaca aggtcgcgtc cgcgctcggc atcggtcgct cgctcgcggc gttccatctc
      361 gacaagcttc tcgagcacgg gctcgtcgag gcgcggtacc ggcgcctgtc gggcaggacc
      421 ggcccgggcg ccggaaggcc gtcgaagctc tatcgcgcga ccgaggagat ccgcgtcacg
      481 gtcccctctc gcgactacga gctcgcggcg cggctgctcg tcgaggcgtc tcggcgcgat
      541 cgtcgactcg agaccgtgga agccgtcgcc ttcgaggccg ggcgcgcggc ggggagcgca
      601 gcggggcgac gcgacgtctc cgcggcggcg gtcaggcggc ggcttcggcg gctgctcgcg
      661 gagcgcgggt acgagccgca cgtggcgcgc ggcgacatcc ggctccgcaa ctgccccttt
      721 cacgcgctcg cggaggagta ccgcgacact atctgtccca tgaaccgggc cctgatcacg
      781 gggatgctcg acgggctggg cgcacaggat ctggaagccg tgccgcagca ggtctccggg
      841 gagtgctgcg tcgccatccg cgcgaccgga tcctcgacgc gaagcgaagt gaggcatcgg
      901 cgtaagactt ctctgaatta gttttagaag cggcgggaag ggtacgaaga gggcgcgatg
      961 cgtcgggcga gggacggagc tggcgggccc gggcggctcg cgcggtcgct tccgcccctg
     1021 acgctcgccg gcatcgcggc catcgccgtc gggctcgtga tcgacgtcgc gtatcacatg
     1081 ctcgatcgcg aagcggagct cggcgctcca tgctgcggac ctgggttcgc aggacacgtc
     1141 gtcacgctcg cggggatggc gcttgcgctc gtcggtgtcg tcgccttcgc gatccgtacg
     1201 agcccgaagc ggggctcccg cacgaaagga ggaggtcgtg ctcgcccaga tctctctgtc
     1261 cgataacccg tgttgccagt tcatcaacga cgtcccgcag tggttcttcc tgctcgtcca
     1321 cgtggcgatc ttcgtcgtcg cggccgtgct ggcggtccgc gccttcggcg tggatgcgcg
     1381 aggcgtcggc tgggggttcg cgctgttcgc cctcgcggag gtgagctacc tcacgtatca
     1441 cctgaacgtc acgctcttcc tgctcgcgca cacggtttct gaggtcttcg tcctgatcgg
     1501 gatcctcctc atcggtgcgg gcgtcgtccg gaaggggacg gcgaggtagc acatggacga
     1561 gcggacgcgg aagcgaacgc gagcccgacg tctcgcctct gcggtcgtgc tcgccgtcgg
     1621 gctcgtcgcc tgcacgagcg gcggatcggg tcccgtcgcg gacgagcagc cggttcggac
     1681 ggacacggtc gacctgccga agagctaccg cttcgagccc gccgttatcg aggtggacgc
     1741 ggggaccacg gtcacgtgga cgaacaacga cgacttccct cacaacgtcc acctgctcgg
     1801 cgggagagac gtcacggagc cgctcccggt cggggggacg gcgacgatca cgttcgacga
     1861 gccgggagag gtcccttacg aatgttcgct gcatccgcag cagatgaagg ggacggtgat
     1921 cgtcacgtga tgcgtgccgc ggaagcggcc cggatgtgtg ggaggcgtga tggccggtaa
     1981 cgcgacgacc ggagcggcgc ggctctccgg acgcgacgtc gcgaagttca ccgcgctcgt
     2041 ggcgctgctc gcgatcttct ccgacgctgc gctcgggcac gggctgacgt gggagaacga
     2101 cccctactgg acgtactggg tcacgaagac gttcctgatc gcgacggtct tcggcctcgg
     2161 caccgcgtgg gtcggcatcg gggccggcag gggcgcggcg atcacactcg tccatacggt
     2221 cgtgctcacc gtctactact ggacgctctc accgatcggg ctcccctcgt ccccggtgtg
     2281 gctcgacctc gagcacacct ggatcaccgg cgtcccgatc cacttcgccg tgatctacct
     2341 cggctacctt gccgcgctgt ggctctggcg gcgccgcggc gcggccgatg tcgaggaacg
     2401 cacggaccgc gcgccgttcg ggacctcggc cctcgtcgtc gggctggcga tcacgatcgc
     2461 cgccggcggg atcgcgagcc tcgcgctcac cgacttcccc ggcgtgacgt ggttcctgac
     2521 gcgcgtcctg atca
//
LOCUS       DASZOB010000517         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_38111, whole genome shotgun sequence.
ACCESSION   DASZOB010000517 DASZOB010000000
VERSION     DASZOB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38111"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VG709_08080"
     CDS             complement(<1..197)
                     /locus_tag="VG709_08080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074206.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HWC33069.1"
                     /translation="MGTFLNLDDASDEHLVGMYQDGVPPAMDALLERYRGFTRMKARS
                     YFLAGADKDDIGQEGMIGLYKA"
     gene            complement(348..1163)
                     /gene="rlmB"
                     /locus_tag="VG709_08085"
     CDS             complement(348..1163)
                     /gene="rlmB"
                     /locus_tag="VG709_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWC33070.1"
                     /translation="MTRGRSGRRRTRPVDVTIGGRRPVLEAVRAGLAREVLVARSARA
                     TAALRDLLDAASTLGVPVRTLDAREIDALVPERNQGVATRVRMPQPLVEAELARHGWD
                     DDALVVALDGITDPHNVGAIARSAEAAGAAVLIVRTVRGAAIGPAAVRSSAGALLHLR
                     VAAVANISRALERLKDAGFWIAGLAPEGRPDAQRPPGRLALVVGSEGEGLSRLVRESC
                     DDLVSIPMRGRVGSLNASSAAAVALFGYASGLLLYGAPGRGPTGRIGGKAGIR"
     gene            complement(1160..1645)
                     /gene="ispF"
                     /locus_tag="VG709_08090"
     CDS             complement(1160..1645)
                     /gene="ispF"
                     /locus_tag="VG709_08090"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWC33071.1"
                     /translation="MSDPETHLRFGLGFDSHAPAHDRTLKLGGVTFEGESGLAGHSDA
                     DVVCHALADAVLGAAGLGDIGGHFPETDPGLAGIDGRDLLRRVVELVRERGMRPVSCD
                     VVVVIERPSIAAHRDEIRSTLAEVLGTRQDRISVKGKRPEGLALTDGGAACIALAVLS
                     P"
     gene            complement(1638..2324)
                     /gene="ispD"
                     /locus_tag="VG709_08095"
     CDS             complement(1638..2324)
                     /gene="ispD"
                     /locus_tag="VG709_08095"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWC33072.1"
                     /translation="MKAVVLLLAAGRGTRLGSDRPKALVDVGARSLLDRSLEAISRWT
                     GVDGVVVAAPPGNVDDVSEVARRGTVEIVAVVEGGATRQESVARALDAVPQGCDVVVC
                     HDVARPFATAALCAAVLDALGDADGAVPAMPVSDTVKRVSGTVVVETVPRDDLVAAQT
                     PQAFRRGALEDAHRDARAAAVVSTDDAALLERLGMRVVVVPGDPMNIKITVPKDVAAA
                     EAITARLGDV"
     gene            complement(2367..2879)
                     /locus_tag="VG709_08100"
     CDS             complement(2367..2879)
                     /locus_tag="VG709_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004806940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWC33073.1"
                     /translation="MFRKGDKVIYPHHGAAVIEDLTEREFLGEKKKYFVLRLAYGDLT
                     LMVPVDSTDEVGIREVVSKREVKKVLDVLRENESGMPTNWSRRYKTNIEKIRSGDVFQ
                     VAEVVRNLAIREGEKGLSAGEKRMLAKARQILVSELVFAVGATEDKAEAMIDKVLDES
                     HGTTLQSQTT"
     gene            complement(2981..>3243)
                     /locus_tag="VG709_08105"
     CDS             complement(2981..>3243)
                     /locus_tag="VG709_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33074.1"
                     /translation="PPPRSAFLGEVSLTGRVRQAPNVSARLRAAAAAGVETVFAAADE
                     RGPGDMRVVPVRNVVEALRWCRGSDAGTSGSRRSSSREAALT"
BASE COUNT          499 a         1187 c         1081 g          476 t
ORIGIN      
        1 gccttgtaga gcccgatcat tccttcctgc ccgatgtcgt ccttgtcggc accggcgagg
       61 aagtaggaac gggccttcat acgggtgaag cctcgatacc gctcgaggag cgcgtccatc
      121 gcgggtggga cgccgtcctg atacatcccg acgaggtgct cgtcggaggc gtcgtccaga
      181 ttcaggaagg tccccaccct aggcgcagcc cgcatgcgtg ttggcactcg ccgtatctcc
      241 tcgtcgctcg gcggcgggcc cacctcagct cgccgattat gtgctcggta cccttctacc
      301 ggcaaggggc aaacaaccgg aggaagcgct taactcggac aaacagatta ccggattcct
      361 gcttttccgc ctatccgtcc cgtcggaccc cgtccgggtg cgccgtaaag gaggagcccc
      421 gacgcgtacc cgaatagcgc gactgccgcc gctgacgacg cgttcagcga cccgacgcgg
      481 ccccgcatcg ggatcgagac gaggtcgtcg cacgactcgc ggacgagccg cgaaagcccc
      541 tccccctccg acccgacgac cagcgcgagg cgtccgggtg gcctctgcgc gtccgggcgg
      601 ccttccgggg cgagccccgc gatccagaac ccggcgtctt tgagtcgctc gagcgcacgc
      661 gagatgttcg cgaccgccgc aacgcggagg tggagcagcg caccggccga ggacctgacg
      721 gccgccggac cgatcgcggc gccccggacc gtccgcacga tcagcactgc cgcgccggcc
      781 gcctcggcgc tacgagcgat cgccccgacg ttgtgcgggt cggtgatgcc gtcgagcgcg
      841 acgacgagcg cgtcgtcgtc ccaaccgtga cgagcgagct ccgcctcgac gagcggttgc
      901 ggcatcctca ctcgagtcgc gacgccctgg ttccgttcgg gaacgagagc atcgatctcg
      961 cgggcgtcga gcgtgcgaac cggcacaccg agggtcgacg ccgcatcgag taggtcgcgc
     1021 agcgctgccg tcgcgcgagc gctccgggcg acgaggacct cacgtgccag cccggcgcgc
     1081 actgcctcga gcacggggcg gcgcccaccg atcgtgacgt cgacgggacg cgtccgccgc
     1141 cggcccgaac ggccgcgggt cacggggaga gcaccgcgag cgcgatgcac gcggcgccgc
     1201 catcggtcag agccagcccc tcgggccgct tgcccttcac cgagatgcgg tcctgacgcg
     1261 tccccagaac ctcagcgagc gtcgaacgga tctcgtccct gtgagcggcg atcgacggcc
     1321 tctcgatcac gacgacgacg tcgcacgaaa ccggtcgcat cccgcgctcg cgcacgagct
     1381 ccacgacgcg gcgcaggaga tctcgcccgt cgatcccggc gagtccgggg tccgtctccg
     1441 ggaagtgacc gccgatgtcg ccgaggccgg ccgcaccgag cacggcgtcg gcgagcgcgt
     1501 gacagacgac gtccgcatcg gagtgccccg cgagccccga ttctccctcg aacgtcacgc
     1561 cgccgagctt cagcgttcgg tcgtgggccg gggcatgcga gtcgaatccc aggccgaacc
     1621 gcaggtgcgt ctccgggtca gacatcgccg aggcgagccg tgatcgcttc ggcagcggcg
     1681 acgtccttcg gaaccgtgat cttgatgttc atcggatccc cggggacgac gacgacgcgc
     1741 atcccgagcc gctcgagcaa ggccgcgtcg tcggtcgaca cgacggccgc ggcgcgcgcg
     1801 tcgcggtggg cgtcctcgag cgcaccgcgc cggaacgcct gcggcgtctg ggccgcgacg
     1861 agatcgtccc ggggcaccgt ctcgacgacg accgtaccgc tcacgcgctt cacggtatcc
     1921 gagacgggca tcgcggggac cgcgccgtcg gcgtcaccga gcgcatccag cacggcggcg
     1981 cagagggcgg cggtcgcgaa cgggcgcgcg acgtcgtggc acacgacgac gtcgcaccct
     2041 tgcggcaccg cgtcgagcgc gcgagcgacc gattcctgcc gcgtcgcgcc gccctccacg
     2101 acggcaacga tctcaacggt tccgcgtcgc gcgacctcgc tcacatcgtc gacattcccc
     2161 ggaggcgccg ccacgacgac gccgtcgacg ccggtccacc gcgagatcgc ctccagcgac
     2221 cgatcgagga gcgatcgggc ccccacgtcg accagcgcct tcgggcggtc ggagccgagc
     2281 cgcgtcccgc ggccggcggc gagcaaaagg acgacggcct tcatcgcttc aggagagatc
     2341 ggacgacgct cggccctcgg gcagcgtcag gtggtctggc tctggagcgt ggtgccgtgc
     2401 gattcgtcga ggaccttgtc gatcatcgcc tcggccttgt cctcggtcgc gccgacggcg
     2461 aacacgagct cggagacgag gatctgtcgc gctttcgcga gcatccgctt ctcgcccgcg
     2521 gagagcccct tctccccctc gcggatcgcg aggttccgga cgacctcggc gacctggaag
     2581 acgtcgccgg aacggatctt ctcgatgttg gtcttgtagc ggcggctcca gttggtcggc
     2641 atccccgact cgttctcgcg gaggacgtcg agcaccttct tgacttcgcg cttgctcacg
     2701 acctcgcgga tgccgacctc gtcggtgctg tcgaccggga ccatgagcgt gaggtcgccg
     2761 tacgcgaggc ggaggacgaa gtacttcttc ttctctccga ggaattcccg ttccgtcaga
     2821 tcttcgatga ccgctgcgcc gtggtgtggg tagatgacct tgtcgccttt tcggaacacg
     2881 tcgccccttt gcgatcggat gaacgggagc tgccgactcc ccattctagc agggcgacca
     2941 cgcgccccgc aaccctaaaa ccgcaggtca gccccgtttt ttacgtcagc gcggcttccc
     3001 tcgaactgga ccgtctcgaa ccggacgttc cggcatcgga gcccctacac caccgcagcg
     3061 cctcgacgac gttccgaacc ggtacgaccc gcatgtcgcc cgggccgcgt tcgtcggctg
     3121 ccgcgaacac cgtctcgacg cctgcagcgg cagcggcccg aaggcgcgcc gagacgttgg
     3181 gcgcctgccg aacgcgtccc gtgagcgaca cctcacccaa gaacgcggaa cgcggagggg
     3241 gcg
//
LOCUS       DASZOB010000518         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_17937, whole genome shotgun sequence.
ACCESSION   DASZOB010000518 DASZOB010000000
VERSION     DASZOB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17937"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VG709_08110"
     CDS             <1..1013
                     /locus_tag="VG709_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC33075.1"
                     /translation="SPPAGAEGLPEARRRSRFLRLRTIAKQVASALLTLLVISIVTFA
                     ATNVKSPEDVARNALGRETTDEQLEAYLEERGLDRPIHVRYVDWLADFVRGDWGVSAV
                     TNRAVRDDILPRFEKTLILSLVSLLVALPISVLIAVFMARRVDSAADFVLLMGTVVVA
                     ALPEFVVGIGLRLLFAVTLGWLPVDSTGLAFATTFTDQAKAYVLPALTLVLAMVPYIV
                     RIGRAAAREALAAPYVQAALLRGLSRRSITWDHAMRNAAVPMVNAVAINIVYLLSGVI
                     VVENVFAFPGIGQLLVQAIGSGDTTMVQSIALVMGAMFILISLAADFLVVYFNPRLKA
                     AH"
     gene            1015..1926
                     /locus_tag="VG709_08115"
     CDS             1015..1926
                     /locus_tag="VG709_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWC33076.1"
                     /translation="MSVAPGAALDVPREQIVEEEAAPPSIWRTLLTSSEGRVGIALGL
                     LMLALILFGRFFAPFSPTEIGTGPPASGPTADNILGTDHLGRDVLSRFLAGGDTILVV
                     PLIAVTLAFLAGGGLGMLGGYLRGWPDAVITRTFDLLLTLPPLLLVLVVIAGLGTSRV
                     VLVVTVALVFLPRMGRVVRGATQAVVTNDYVAAAQARGERTSAIVVREVLPNIAAPAI
                     ADFALRITYAIIFVATLNFLGLGAQPPAPDWGLMVSESRAFIAVAPWATLAPAVGIAA
                     LSVAFNFVADALTRHLTRESAKGVVPL"
BASE COUNT          235 a          744 c          664 g          344 t
ORIGIN      
        1 cctcacctcc ggccggcgcg gagggccttc cggaggcgcg taggcggtcg cggttccttc
       61 ggctgcggac gatcgcgaag caggtcgcgt cggcgcttct caccctcctc gtcatctcca
      121 tcgtcacgtt cgccgccacg aacgtgaagt cgcccgagga cgtggcgcga aacgcgctcg
      181 gccgtgagac gacggacgag cagctcgagg cgtacctcga ggagcgcggg ctcgaccggc
      241 cgatccacgt gcgctacgtg gactggctcg ccgacttcgt gcgcggggac tggggcgtgt
      301 cggcggtgac caaccgagcc gtccgcgacg acatcctccc gcgcttcgag aagacgctca
      361 tcctctcgct cgtgtcgctg ctcgtcgcgt tgccgatctc ggtcctgatc gccgtcttca
      421 tggcgcggcg cgtcgacagc gccgcggact tcgtccttct gatgggaacc gtcgtggtcg
      481 cggcgttgcc ggagttcgtg gtcggcatcg ggctccgctt gctcttcgcc gtgaccctcg
      541 ggtggttacc cgtcgactcg accggtctcg ccttcgcgac gacgttcacc gaccaggcga
      601 aggcgtacgt ccttcccgcg ctcacgctcg tgctcgcgat ggttccgtac atcgtccgca
      661 tcggccgcgc cgcggcgcgc gaggcgctcg ccgcgccgta tgtgcaggcg gccctcctcc
      721 gcggcctctc ccgccgctcg atcacctggg accacgcgat gcggaacgcc gccgtgccga
      781 tggtgaacgc ggtcgcgatc aacatcgtct acctcctgag cggcgtgatc gtcgtcgaga
      841 acgtgttcgc gttccccggg atcggtcagc tcctcgtgca ggcgatcggc agcggcgata
      901 cgacgatggt gcagtcgatc gcgctcgtga tgggtgcgat gttcatcctc atcagcctcg
      961 cggcggactt cctcgtcgtc tacttcaacc cgcggctcaa ggccgcgcac tgacatgagc
     1021 gtcgctcccg gagccgcgct cgacgtcccg cgcgagcaga tcgtcgagga ggaggccgcg
     1081 ccgccgtcga tctggcggac gctcctgacg agctccgagg gccgggtcgg gatcgcgctc
     1141 ggcctcctca tgctcgcgct gatcctgttc gggcgcttct tcgccccgtt cagcccgacc
     1201 gagatcggca ccgggccccc cgcgagcggg ccgacggccg acaacatcct cggcaccgac
     1261 catctcggcc gcgacgttct cagccgcttc ctcgcgggcg gcgacacgat cctcgtggtt
     1321 ccgctgatcg ccgtgacgct cgcgttcctc gcaggaggcg gcctcgggat gctcggcggc
     1381 tacctgcgcg gatggccgga cgcggtcatc acgcggacgt tcgacctcct cctgacgctt
     1441 ccgccgctgc tgctcgtcct cgtcgtcatc gccgggctcg ggacgtcgcg cgtcgtgctc
     1501 gtcgtgacgg tggcgctcgt cttcctgccg cgcatggggc gcgtcgtccg cggcgccacg
     1561 caggcggtgg tcacgaacga ctacgtcgcc gcggctcagg cccgcggcga gcggacgagc
     1621 gcgatcgtcg tccgcgaggt gctcccgaac atcgccgcgc ccgcgatcgc cgacttcgcc
     1681 cttcgcatca cgtacgcgat cattttcgtc gcgacgctca acttcctcgg tctcggcgcc
     1741 cagccgcccg cgccggactg gggcctcatg gtgtcggagt cgcgggcgtt catcgcggtc
     1801 gccccgtggg cgacgctcgc gccggccgtc gggatcgccg cgctctcggt cgcgttcaac
     1861 ttcgtcgccg acgcgctgac gcgacacctg acccgcgagt ccgcgaaggg agtcgtcccg
     1921 ctgtgagcac gaacccgccg gacgcccacg acgccacggt cccggcccgg ccgcgcgacc
     1981 gcggtat
//
LOCUS       DASZOB010000519         3660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191037, whole genome shotgun sequence.
ACCESSION   DASZOB010000519 DASZOB010000000
VERSION     DASZOB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191037"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VG709_08120"
     CDS             complement(<1..591)
                     /locus_tag="VG709_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWC33077.1"
                     /translation="MPHGKMGWNSTLSMAGGGMIGGGIFSVLGVVVALAGPLAWTSFV
                     AAGAVALITSMSYVRLAAHFHEAGGAFSYLRGAGHPAIAGTLSWILIGGYVLTIAVYG
                     FTFGHYLGAALGIAQPWITVAAVGVVVVFVGVNLLGVGEASGVEVVLVWGKVAVLLVL
                     AAIGLASWNPDALGQGVQGGGVGGTLLGAASVFMAYE"
     gene            complement(652..1566)
                     /locus_tag="VG709_08125"
     CDS             complement(652..1566)
                     /locus_tag="VG709_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33078.1"
                     /translation="MTAAGGEPAGERQDGLVPSLREVRGTQLARFGRYTRRFAALYER
                     ARRWDRPEFVTADWRARNRALERRLFPTPPARFLHADEIRYQMFVGDRCLAEQVAFLR
                     ILGHDASVLAEDAAGDPPMLVEVDGVVTSANTVHHVHHLRRFEIATSVACAAVGTVLE
                     WGGGYGSLAKLFRRIHGGAPTYAIIDTPLFSCVQWLYLSSVFGDDHTHVLLDRRDTLL
                     PGRFNLVPLALASSLEIEPQLFVSTWALNESAVDVQAMVVERRWFGAPHLLLGMHDGD
                     PLIAKATRDGARAEPVGACMPAQRYLFR"
     gene            complement(1563..2483)
                     /locus_tag="VG709_08130"
     CDS             complement(1563..2483)
                     /locus_tag="VG709_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC33079.1"
                     /translation="MRGTVCDVVVITRDRVETLLRTLANLTRLDDRGRIIVVDNASTD
                     ATAELVAERFPDVSFIRLGRNHGAAARNGGVGASHAPFVALTDDDSWWAPGALEQAAR
                     ALERYPRLGLVAARVLVGPKNVPDPTNALMEEALVGADGGLPGRAVLGFVACGAIVRR
                     EAFLEVGGFQQRYGIGGEEQLLAIDLAARGWGVRYLRDVVVHHHPAAGNRDGRRRRIV
                     RNDLWTAWLRRPLPAAISRTTRILASSPTDADTWRGLAGAIRGLPWVVADRRVVPPSV
                     ERHLRALDRAAARRGRSTRHRIEAATDGAG"
     gene            complement(2628..2975)
                     /locus_tag="VG709_08135"
     CDS             complement(2628..2975)
                     /locus_tag="VG709_08135"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWC33080.1"
                     /translation="MRLSELLTFEVVDERGRSLGRVRDVRLDADRDEAERSPPRDLAG
                     THLLVGSGFVAERLGYAYGPVRGPAIVRWLMKRWAHGLRVVPVADVAQVSAGRIVASV
                     DREGLPPLERRDD"
     gene            complement(2962..3342)
                     /locus_tag="VG709_08140"
     CDS             complement(2962..3342)
                     /locus_tag="VG709_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33081.1"
                     /translation="MSDQLHACLDLLDRQVLDADDEPVGKVDDVELDWDGPRLRVHAL
                     LVGPHALGRRMGGRIGRWLTGAANRLSGTNEPVRIPLKAVAEIGVLIKLNVRAQDVER
                     IHGVEHWLRDRLIGRIPGSHHASE"
     gene            complement(3342..>3660)
                     /locus_tag="VG709_08145"
     CDS             complement(3342..>3660)
                     /locus_tag="VG709_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33082.1"
                     /translation="SWGKFRPPASASRFSTLLLVTLLGASAFALTTVDPIKITIYAVY
                     LGAATLPITYGSILVVANDRRYMGSLANGRLGNTIASIYLVLLTAVALAAFPLLLITK
                     GGA"
BASE COUNT          583 a         1317 c         1282 g          478 t
ORIGIN      
        1 ctcgtacgcc atgaacaccg acgcggcgcc gagcagcgtg ccgccgaccc ccccgccttg
       61 gacaccctga ccgagcgcgt cggggttcca cgacgcgagc ccgatggccg ccagcacgag
      121 caggacggcg accttccccc acacgaggac gacctcgacg cccgacgcct cgccgacgcc
      181 gagcagattc accccgacga agaccaccac cacgcccacg gctgccacgg tgatccaggg
      241 ctgcgcgatc ccgagcgcgg ctccgaggta atggccgaac gtgaagccgt acaccgcgat
      301 cgtgaggacg tagccgccga tgaggatcca cgacagcgtt ccggcgatcg caggatgtcc
      361 cgcgccccgg aggtacgaga acgcgccgcc ggcctcgtgg aagtgcgccg cgagacgcac
      421 gtagctcatc gaggttatga gggcgacggc gccggccgcg acgaagctcg tccatgccag
      481 tggcccggcc agcgcgacga cgacgccgag gaccgagaag atcccgccgc cgatcatccc
      541 gccgcccgcc atcgacaacg tcgagttcca tcccatcttg ccgtgcggca tgcgacgttg
      601 gttcccgttc cgcgaggacc tactcgggag gcgtctcggt gtctcgatcg ctcagcggaa
      661 caagtagcgc tgcgcgggca tgcaggcgcc tacgggctca gcgcgagcgc cgtcgcgcgt
      721 cgctttcgcg atgagcggat ctccgtcgtg catgcccagc aggagatgcg gcgcgccgaa
      781 ccaccggcgc tcgacgacca tcgcttgaac gtccacggcg ctctcgttga gcgcccacgt
      841 cgagacgaag agctgaggct cgatctcgag tgacgatgcg agcgcgagcg gcacgaggtt
      901 gaagcggccg ggcaggagtg tgtcgcggcg gtcgagcagg acgtgcgtgt ggtcgtcgcc
      961 gaagaccgac gagagataga gccactgcac gcacgagaac agcggcgtgt cgatgatcgc
     1021 gtatgtgggc gcgcccccgt ggatccgcct gaacagcttc gccaggcttc cgtacccgcc
     1081 gccccactcg agcacggtgc cgaccgctgc gcacgcgacg ctggtcgcga tctcgaaccg
     1141 ccggaggtgg tgcacgtggt ggacggtgtt cgccgacgtc acgacgccgt cgacctcgac
     1201 gagcatcgga gggtctccgg ccgcgtcctc ggcgagcacc gaggcgtcgt gtccgaggat
     1261 ccggaggaac gcgacctgct ccgcgaggca ccgatcgccg acgaacatct ggtaccgaat
     1321 ctcatcggcg tggaggaacc gtgcgggcgg tgtcgggaag aggcgccgct cgagcgcccg
     1381 attccgcgct cgccagtcgg ccgtgacgaa ctcggggcga tcccaccgcc gtgcccgctc
     1441 gtacagcgcg gcgaagcggc gcgtgtaccg gccgaaccgc gcgagctggg tgccgcgcac
     1501 ttcgcgcagc gaggggacga ggccgtcttg tcgctcgccc gcgggctctc cccccgcggc
     1561 cgtcatcccg cgccgtcggt cgccgcttcg atccggtgtc gcgtgctgcg cccgcgacgc
     1621 gcggccgcgc ggtcgagcgc gcgaaggtgg cgttcgacgg acggcggcac gacccggcga
     1681 tcggcgacta cccacggcag gccccggatc gcgccggcga gcccacgcca cgtgtccgcg
     1741 tcggtcgggg agctcgcgag gatccgggtc gtccgcgaga tcgctgcggg cagcggtctg
     1801 cggagccacg cggtccagag gtcgttccgc acgatgcggc gacggcgccc gtcgcggttc
     1861 ccggccgccg ggtgatgatg cacgacgacg tcgcggaggt accggacgcc ccatccgcgc
     1921 gccgccaggt cgatcgccag gagctgctct tcgccgccga tcccgtagcg ctgttggaac
     1981 ccgcccacct ccaggaacgc ctccctccgc acgatggcac cgcacgccac gaacccgagg
     2041 acggcacgcc ctgggaggcc gccgtccgcc cctaccaggg cctcctccat gagggcgttc
     2101 gtcggatccg ggacgttctt ggggcccacc agaacgcgcg cggcgacgag gccgagacgt
     2161 gggtatcgct cgagcgcccg agcagcctgc tccagtgcgc ccggcgccca ccatgagtcg
     2221 tcgtcggtaa gcgcgacgaa cggcgcgtgc gacgcgccga ccccgccgtt ccgcgccgcg
     2281 gcgccgtggt tcctcccgag cctgatgaat gagacgtccg ggaagcgctc tgcgacgagc
     2341 tcggcggtcg cgtcggtcga cgcgttgtcg acgacgatga tgcgtccgcg atcgtcgagc
     2401 cgcgtgaggt tcgcgagcgt gcggaggagc gtctcgacgc ggtcacgggt gatcacgacg
     2461 acgtcacaga cggttccgcg cacggcggcg acgatgctac ccatacggtc gcggcgcaag
     2521 ccggggcacc cgcgtccgcc ggctagcggc cgcggcggag aacgatccac gcaagcaagc
     2581 cgaccaagag caaccactgc gcccacgcgg cgaggaccgc gaggccgtca gtcatctcgg
     2641 cgctccagcg gaggaaggcc ctcgcgatcc acggacgcga cgatgcgccc cgctgatacc
     2701 tgcgcgacgt cggcgaccgg aacgaccctc agcccatggg cccaccgctt catcagccac
     2761 cggacgatcg ccggaccccg gacgggtccg tacgcgtatc cgaggcgctc cgcgacgaag
     2821 ccgctgccga ccaggagatg cgtgcctgcg aggtcgcgtg gcggcgatcg ctccgcctca
     2881 tcgcggtccg cgtcgagccg gacgtcgcgg acgcggccga gcgagcggcc gcgctcgtcc
     2941 actacctcga acgtgagcag ctcactcaga cgcatggtgg ctcccgggga tcctgccgat
     3001 caggcgatcg cgtagccagt gctccacgcc atggatccgc tcgacgtcct gcgcgcggac
     3061 gttgagcttg atgaggacgc cgatctcggc caccgccttc agcgggatgc ggaccggctc
     3121 gttcgtgccc gagagacggt tcgcggctcc ggtcagccac cgaccgatcc gcccccccat
     3181 ccggcgcccg agcgcgtgtg gaccgacgag gagagcgtgg acgcggagcc tcggtccgtc
     3241 ccagtcgagc tccacgtcgt cgaccttccc cacgggctcg tcgtcggcat cgaggacctg
     3301 gcggtcgagg aggtcgaggc acgcgtgcag ctggtccgac atcaagcgcc gcccttcgtg
     3361 atgaggagca gcgggaaggc ggcgagcgcg acggcggtca gcagcacgag atagatcgac
     3421 gcgatcgtgt tgccgagcct gccgttcgcg agcgacccca tgtaccgccg gtcgttcgcg
     3481 acgacgagta tcgatccgta cgtgatcgga agcgtcgcgg cgccgaggta gacggcgtaa
     3541 atcgtgatct tgatcgggtc gaccgtcgtc aacgcgaacg cgctcgcccc gaggagcgtg
     3601 acgagcagaa gcgtagagaa ccgggacgcg gaggccggcg gccggaactt tccccatgac
//
LOCUS       DASZOB010000520         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191095, whole genome shotgun sequence.
ACCESSION   DASZOB010000520 DASZOB010000000
VERSION     DASZOB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191095"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VG709_08150"
     CDS             complement(<1..276)
                     /locus_tag="VG709_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWC33083.1"
                     /translation="MRIALVCPYAWDAPGGVQVHVRQLARRLRERGHDVLVVAPARDG
                     VEERDVAIVGRTIGIPINGSVAPVCLALASARKLRSALHGFRPDGGHV"
     gene            complement(273..1214)
                     /locus_tag="VG709_08155"
     CDS             complement(273..1214)
                     /locus_tag="VG709_08155"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HWC33084.1"
                     /translation="MSARETPKERLAYHAYATAERLAMRLPERWSQALFRGVADAAFA
                     VAPGVRRVVEANIGRVMGRDPGSPLVRATTREAFRSYARYWYDTFRLRGMPDEEVLRR
                     MRCEGIERLERAADDGRGAILALPHLGNWDAAGKWVHLRGWRITAVAEVLRPERLFEL
                     FLRHRRELGMNIVPLWDARKVGEELVALVAQNHFIALVSDRDLKGRGVEVEMFGAKRR
                     LPVGPALLSLATGSPLHACAVYDTDDGWTIFIGERLEVERTGQLRRDVTALTQKLAAA
                     FERSIEAAPTQWHMFQPAWEEEPEAVVADVERSAAPA"
     gene            complement(1211..1759)
                     /locus_tag="VG709_08160"
     CDS             complement(1211..1759)
                     /locus_tag="VG709_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33085.1"
                     /translation="MAGSPSAVGGGAGVRPAAVLAPLALAVASAATASVLVLIGQPLA
                     AAMATAVAAGALIFGGIRAREAPASRLPFADAVAERAVDVAVLGGFAWAALPEDPAAA
                     AAALTALVSSYLASYLRAKATGLGFAVDEPAFVRPLRLVLVVLALFRPENAAVALWAA
                     AAVAAQSIVRGAALVARKREAP"
     gene            1839..>2816
                     /locus_tag="VG709_08165"
     CDS             1839..>2816
                     /locus_tag="VG709_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWC33086.1"
                     /translation="MKTYQPNQIRNVLLVGHGGAGKTTLAEALLYVGGAITRMGKVED
                     GNTVTDFEPEETRKNISVSLALAPLEWRGTKLNVLDAPGYADFVGEMSAAMRVADAAV
                     FVVSGVEGLEVQTQVAWSLADQLRLPRFLFVNKLDRENSSFRRTLEQMREAFGLGAAP
                     ISLPLGREHDFRGVISVIDEAAFAYDSSGNSTEEPVPDDRRERVDEVRTNLLDSGAET
                     NDELLEKYLESGELTRDEIVSSLHDGIVEATIFPVVVGSATSLIGVDRLADVLVAAGP
                     SPLDRPPVESETGDAREAKPDAPMSALVFKTMTDPYVGRVSFFKTYSGIV"
BASE COUNT          443 a         1026 c          977 g          370 t
ORIGIN      
        1 gacgtggccg ccgtccggcc ggaaaccgtg aagtgccgag cggagcttcc gggccgaggc
       61 gagcgcgagg cacaccggcg cgacggaccc gttgatcggg atgccgatcg tgcgaccgac
      121 gatcgcgacg tcgcgctcct ccacgccgtc gcgcgcgggg gcgacgacca ggacgtcgtg
      181 accacgctcg cgcagccgtc gcgcgagctg gcgaacgtgt acctgcacgc cacccggcgc
      241 gtcccacgcg tacggacaga cgagcgcgat cctcatgcgg gcgccgccga ccgctcgacg
      301 tcggccacga ccgcctcagg ctcctcctcc catgccggct ggaacatgtg ccattgcgtc
      361 ggcgctgcct cgatcgagcg ctcgaacgcc gccgcgagct tctgggtcag cgcggtcacg
      421 tcgcgacgaa gctgcccggt gcgctcgacc tcgaggcgct caccgatgaa gatcgtccag
      481 ccgtcgtcgg tgtcgtagac cgcgcacgcg tggagcggcg accccgtcgc cagcgacagg
      541 agcgccggtc cgacgggcag cctgcgtttc gcgccgaaca tctcgacctc gaccccgcgt
      601 cccttgaggt cacggtcgga cacgagcgcg atgaagtggt tctgcgcgac gagcgcgacg
      661 agctcctcgc cgaccttccg tgcgtcccac agcggcacga tgttcatccc gagctcccgg
      721 cgatgacgga ggaacagctc gaacagccgc tcgggacgca gcacctcggc gacggccgtg
      781 atccgccagc cgcggagatg tacccacttc cccgcggcgt cccagttgcc gagatgcggc
      841 agcgcgagga tcgcgccgcg tccgtcgtcc gcggcccgct cgaggcgctc gatcccctcg
      901 caccgcatcc ggcggaggac ctcctcgtcc ggcatgccgc ggagccggaa cgtgtcgtac
      961 cagtagcgcg cgtacgatcg gaacgcctcg cgcgtcgtcg cgcgcacgag cggagatccg
     1021 ggatctcgcc ccatcactcg tccgatgttc gcctcgacga cccgacgcac ccccggcgcg
     1081 accgcgaagg cggcgtcggc gacgccgcgg aagagcgcct ggctccagcg ttccggcagc
     1141 cgcatcgcga gccgctccgc cgtcgcgtac gcgtggtacg cgagccgctc cttcggcgtc
     1201 tcacgcgcgc tcacggcgcc tcacgcttcc gggcgacgag cgccgcccct cgaacgatcg
     1261 actgggccgc gaccgcggcc gcggcccaga gcgcgacggc cgcgttctcg ggacggaaga
     1321 gcgccaacac gaccaacacg aggcgcagag gacgtacgaa cgctggctcg tcgaccgcga
     1381 accccagccc ggtggccttc gcccgcaggt acgacgcgag atacgaactg acgagcgcgg
     1441 tgagcgccgc ggcagcggcg gccggatcct cgggcagcgc cgcccatgcg aatcccccga
     1501 gcaccgccac gtcgaccgcc cgctcggcga ccgcatcggc gaacgggagc cgcgacgcgg
     1561 gcgcctcccg cgcacggatg ccgccgaaga tgagtgctcc tgccgctacg gcggtcgcca
     1621 tcgcggccgc gagcggctgc ccgatgagga cgaggaccga cgctgtcgcc gctgaggcga
     1681 cggcgagcgc gaggggcgcg agcaccgcgg cgggccgcac gcccgcgcca ccgcccacgg
     1741 cggacggcga acctgccatg gcgagcgcga gcgtagcacg gtgcctccca ggggcctctc
     1801 tataatcggg actccgcgag cgaccggggg aacctcacgt gaagacgtac cagccgaacc
     1861 agatccgcaa cgtgctgctc gtcggccacg gcggcgccgg caagaccacc cttgcggagg
     1921 cgctcctcta cgtcggcgga gccatcacgc gcatggggaa ggtcgaagac ggcaacaccg
     1981 tcaccgactt cgagccggag gagacccgga agaacatcag cgtctcgctc gcgctcgcgc
     2041 cgctcgagtg gcgcggcacc aagctgaacg tgctcgacgc tccgggctac gccgacttcg
     2101 tcggggagat gagcgccgcg atgagggtcg cggatgccgc ggtgttcgtc gtgtccggcg
     2161 tcgagggcct cgaagtccag acacaggtcg cgtggagcct tgccgaccag ctgcgcctgc
     2221 cccggttcct tttcgtcaac aagctcgatc gggagaactc ttctttccgg cgcacgctcg
     2281 agcagatgcg cgaggcgttc ggcctcggcg ccgcgccgat ctcgctcccg ctgggccgcg
     2341 agcacgattt ccgcggggtc atctcggtga tcgacgaagc ggcctttgcc tacgactcgt
     2401 cgggtaattc caccgaggag ccggttcccg acgacaggcg cgagcgggtc gacgaggtac
     2461 gtaccaacct gctcgactcg ggcgccgaga cgaacgacga actgttggag aagtacctcg
     2521 agagcggaga gctcacgcgc gacgagatcg tgtcgtcgct tcacgacggg atagtggagg
     2581 cgacgatctt cccggtcgtc gtcggttcgg caacgagcct catcggtgtg gacaggctcg
     2641 cggacgtcct ggtcgcggcc ggtccgtcgc ccctcgaccg gcctccggtg gagagcgaga
     2701 cgggcgacgc gcgcgaggcg aagccggacg cgccgatgtc ggcgctcgtg ttcaagacga
     2761 tgacggatcc ctacgttggg cgggtcagct tcttcaagac gtactcgggc atcgtg
//
LOCUS       DASZOB010000521         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_140277, whole genome shotgun sequence.
ACCESSION   DASZOB010000521 DASZOB010000000
VERSION     DASZOB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140277"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VG709_08170"
     CDS             complement(<1..652)
                     /locus_tag="VG709_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33087.1"
                     /translation="MSWKRVGQFVLRRPVATLVASLAVLVPPTFAVPALRTSYDQLRE
                     LPASADSVRGFDALEGSFEPGEIQPLIVMVRSRRALWNDESFEALDDLTVNLGKVRGV
                     TRVRSITRPTGGGVTEAQIQAIGLGDVDALTSRLPRAIRGIDRLVGGLERMRSGLVRI
                     RASVPQQRAGISQGAEGIEAMREGLERISRGLRRLRGGLARAESAVRTLARRVAEPA"
     gene            complement(649..1761)
                     /locus_tag="VG709_08175"
     CDS             complement(649..1761)
                     /locus_tag="VG709_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWC33088.1"
                     /translation="MLRPLARFVSRYAVPVILAWGVLAGALFLLAPPLDEVLTQDIAA
                     FLSDDAPSVVAAEEVGEAWPGEELQNFAAIVFRREEGLTSRDVRYLRGVERWVRSDEA
                     PPNVRTTQSPYSAPEFAEAFRSEDGRVEIVGVSFTTPPFQPDTNEAVEAIRGRAQSSP
                     PAGLAVHVTGNAGVAADQASAIETSVHRTTLITLFLVTAILVWVYRSPIAPAIPLMTI
                     ALAFLVSRGVVALLGAAGMAVSSLVEQLLVVIVFGAGTDYCLFVVSRYKEELTGRALE
                     PEERRRTLVGTMVVIGGLIASSAATTVVGFASQSAAEFGMYRTTGPAIAVAVIITLVA
                     GLTLVPALLSLLGRWAFWPWRASGSAPTLMTGEART"
     gene            complement(1809..>2382)
                     /locus_tag="VG709_08180"
     CDS             complement(1809..>2382)
                     /locus_tag="VG709_08180"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010840264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWC33089.1"
                     /translation="DVASGARVAAERFVARALDEGLVDVAYAEVDSPLGRLVAAVTPR
                     GLVRLSYADERADDVLQELSSRLSPRVLEAPGRLDAVRRELDEYFDGRRRSFALPLDW
                     RLTRGFGRRILRATARIPYGELATYRRVARTAGNERAVRAAGNALGANPIPIVVPCHR
                     VIRTGGALGGYTGGLDRKRFLLELEGVPTD"
BASE COUNT          357 a          876 c          873 g          276 t
ORIGIN      
        1 gcgccggctc ggcaacgcgt cgcgcgaggg ttcgaaccgc ggattcggcg cgcgcgagcc
       61 ccccgcgcag gcggcgaagg ccgcgagaga tgcgctccag gccttcgcgc atggcctcga
      121 tcccctcggc cccctgcgag atcccggcgc gttgctgcgg gaccgacgcg cggatccgca
      181 cgagccccga acgcatccgc tcgaggccgc cgacgagccg gtcgatcccg cggatggcgc
      241 gcggcagccg agatgtgagc gcgtccacgt cgccgaggcc gatcgcctgg atctgggcct
      301 cggtgacgcc cccgccggtc ggacgggtga tcgagcggac gcgcgtcacg ccccgcacct
      361 tcccgaggtt cacggtgagg tcgtcgagcg cctcgaacga ctcgtcgttc cagagggcgc
      421 gccgcgagcg caccatgacg atcagcggct ggatctcgcc gggctcgaac gacccctcga
      481 gcgcgtcgaa cccccggacg gagtccgccg aggcggggag ctcgcgcagc tggtcgtacg
      541 acgttcggag cgcgggcacc gcgaacgtcg gcggcacgag cacggcgagc gacgcgacga
      601 gcgtcgcgac gggtcgccgc agcacgaact gcccgacgcg cttccagctc acgtgcgcgc
      661 ctcccccgtc atgagcgtcg gggcgcttcc cgacgctcgc cacggccaga acgcccaccg
      721 gccgaggagc gagagaagcg ccggcacgag cgtcaggccg gcaacgagcg tgatgatcac
      781 cgcgacggcg atcgctgggc ccgtcgtccg gtacataccg aactcggcgg cgctctggct
      841 cgcgaacccg acgacggtcg tggcggcgct cgaggcgatc agcccgccga tgaccaccat
      901 cgtgccgacg agcgtccggc ggcgctcctc cggctcgagg gcccggccgg tcagctcctc
      961 cttgtaacga gagacgacga acaggcagta gtccgtcccg gcgccgaaaa cgatcacgac
     1021 caggagctgc tcgacgagcg acgagaccgc catcccggcg gcgccgagca gcgcgacgac
     1081 gccgcgtgag accaggaagg cgagcgcgat cgtcatgagc gggatcgcgg gcgcgatcgg
     1141 cgatcggtag acccagacga ggatcgcggt gaccaagaag agcgtgatca gcgtggtccg
     1201 gtgcaccgac gtctcgatcg cgctggcctg gtcggccgcg acgcccgcgt tgcccgtcac
     1261 gtggaccgcg aggccggcgg ggggcgacga ctgcgcgcgg ccgcggatcg cctcgaccgc
     1321 ctcgttcgtg tcgggctgga acggcggcgt cgtgaacgat acgccgacga tctcgaccct
     1381 gccgtcctcc gagcggaagg cctcagcgaa ctccggcgcg gagtacggcg actgcgtcgt
     1441 ccggacgttg gggggtgcct cgtccgagcg cacccagcgc tcgaccccgc ggaggtagcg
     1501 cacgtcccgc gaggtgaggc cttcctcgcg gcggaacacg atcgcggcga agttctggag
     1561 ctcctcgccg ggccaggcct cacctacttc ctcggcagcg accacgctcg gcgcgtcgtc
     1621 ggagaggaag gcggcgatgt cctgcgtgag cacctcgtcg agcggagggg cgaggaggaa
     1681 gagggctccc gcgagcaccc cccacgcgag gatcaccggg acggcgtacc gcgagacgaa
     1741 gcgcgcgagc ggtcggagca tcgaccctcc gttgggctgg ccgatgctac tcgccgactg
     1801 accctcagtc aatcggtggg gacgccctcc agctcgagca ggaagcgctt gcgatcgagg
     1861 ccgccggtgt acccgccgag cgcgcctccg gtgcgaatga cgcggtggca cggtacgacg
     1921 atcgggatcg ggttggcgcc cagcgcgttc cccgccgccc gcacggcgcg ctcgttgccg
     1981 gccgtgcgcg ccacccggcg gtacgtcgcg agctcgccgt aggggatccg cgccgtcgcg
     2041 cggaggatcc gcctgccgaa gccgcgcgtc agccgccagt cgagcggcaa cgcgaagctg
     2101 cgccggcggc cgtcgaagta ctcgtcgagc tctcggcgga ccgcgtcgag ccggccgggc
     2161 gcctcgagca cccgcggcga gagcctcgac gacagctcct ggagcacgtc gtccgcgcgc
     2221 tcgtccgcgt acgacagccg gacgaggccc cgaggcgtca cggccgcgac gaggcggccg
     2281 agcggcgagt ccacctcggc gtacgcgacg tcgacgaggc cctcgtcgag cgcccgggcg
     2341 acgaaccgct cggccgcgac tcgcgcgccg ctcgccacgt cc
//
LOCUS       DASZOB010000522         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191292, whole genome shotgun sequence.
ACCESSION   DASZOB010000522 DASZOB010000000
VERSION     DASZOB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191292"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VG709_08185"
     CDS             <1..720
                     /locus_tag="VG709_08185"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWC33090.1"
                     /translation="DVEGVYTADPRIVPSARKLHAVSYEEMLEMAASGARVLQLRSVE
                     YARGAGILLHVRSSFSDASGTWVREEDERMEQAIISGVTHDDSEAKISIEGIPNDPGV
                     AARVFRAVADEGIGVDMIVQNAVRDGRTDISFTVSKEDLAHAQSVIESIVDEIGAQRF
                     TADAGIAKVSLVGAGMKTHPGVQAEMFAALAEAGVEDIEMISTSSVRITCLIPAAKIE
                     SAVRTLHERFSLSAESAIREH"
     gene            720..1406
                     /locus_tag="VG709_08190"
     CDS             720..1406
                     /locus_tag="VG709_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWC33091.1"
                     /translation="MLVCPTCRSDNQEDAQFCTTCGRSLSPDEAALVAPARREPADLE
                     IDIPPRKPQSPLPGLFALAAVLVAGLGAVTWFALRPNPCAGKFSSERFPYCMAVPRGW
                     QEAQVDVQGTAVDAFTPSDDGPTVFVYAAEAPDGLDTEGYTDDLRSEAQDSGLFPTNP
                     RPIEIGGDDALVWELTKSDTTTGSMITERRVTLVRDGTLWQILLFGNERAVNRSEPQF
                     ERMLETWAWQ"
     gene            1448..2473
                     /locus_tag="VG709_08195"
     CDS             1448..2473
                     /locus_tag="VG709_08195"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWC33092.1"
                     /translation="MRVAILGATGAVGREMLRTLEERSFPLDALVLLSSPRSAGRRLP
                     FRGDDVVVRAVEDDSFEGVDLALFSAGATASQEYAPRAVDDGAVVVDNSSAFRMDPGV
                     PLVIPEVNAEALSAHRGIVANPNCTAITAIMAVAPLHRAAGLRSLVVSSYQSVSGAGH
                     KGVAELLEQVDKLRGDEEDLARPDPAVLPVGEVFGKTIAFNVIPRGGSFEPDGWTTEE
                     TKLVTETRKILELPELAVAATVVRVPVVVGHSVSAVATFDRAMTPDEARGVFARAPGM
                     LVVDDPASDTFPTPLAAAGRDEVLVGRIRSTQGIANAVQLFAASDNLRKGAALNAVQI
                     AELVAAR"
     gene            complement(2470..3381)
                     /locus_tag="VG709_08200"
     CDS             complement(2470..3381)
                     /locus_tag="VG709_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWC33093.1"
                     /translation="MRATGVITTARGTRTEAFGATEWGLLAAIAAMWGSSYLLIAVGL
                     EAFPPTLIALLRLAFGAVAVGAFRRARRPIDREDLPRVALLGVIWMAVPLLLFPIGQD
                     LGIASSVAGMINGAMPLFAALFAWILLARAPAARQGVGLALGFAGVVLILLPSLQGAA
                     ATAVGAALLILAVTLYGLSANITVPLQQKYGALPVVFRAQLVALVLLAPLGLAFGLPR
                     ASFALDALAAMVALGVFGSGLAFIAMATLVGRAGATRGAVAIYFVPVVALALGVTILG
                     ERPAPISLAGVALVLLGAYLTSRREAR"
     gene            3449..3829
                     /locus_tag="VG709_08205"
     CDS             3449..3829
                     /locus_tag="VG709_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC33094.1"
                     /translation="MRSVDYVILYVEELERSIAFYRDVIGLSLRLEGDGYAEFATEGT
                     KFGLFERSKLPELIGRAAAPRGPAAEVVFVVDDVDAEAERLAAAGARVLMPPTDRAWG
                     HRTLHVSDPDGHVVELAQEIPRSS"
     gene            complement(3816..>4623)
                     /locus_tag="VG709_08210"
     CDS             complement(3816..>4623)
                     /locus_tag="VG709_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWC33095.1"
                     /translation="KGRPPSFWDDAPVLSLVAVGTDGSDSASSAVAHAARLAEATGAE
                     LAVVHAYGGTGSLDSPVGTREIGASLLRDVAGRHVPTSRARAVLREGDPADVLLDVAR
                     QEGADLIVVGNRGMGRRPARIGNVPNRIAHRAPCDVLIAQTTRGRRQRTYGRVLLTTD
                     GSPTAGRAVAVGARLASVVGADTVLLHVGADTSVAERTAAELQPDLTVEALTVEGDPA
                     RTIGDVAAERDCDLIVIGNRGMTGLRRFLASVPSRVAHEAPCHVLLVKTT"
BASE COUNT          700 a         1625 c         1671 g          627 t
ORIGIN      
        1 gacgtggagg gcgtttacac ggccgatccc cgcatcgtcc cgtcggcgag gaagctccac
       61 gcggtctcct acgaggagat gctcgagatg gcggcgagcg gcgcacgcgt cctccagctc
      121 cgatcggtgg agtacgctcg cggggctggg atcctgctgc acgtccgctc ttcgttctcg
      181 gacgcgagcg gcacgtgggt acgggaggaa gacgagcgca tggagcaggc catcatctcc
      241 ggggtcaccc acgacgactc ggaggcgaag atctcgatcg agggcatccc caacgatccc
      301 ggcgtggccg cgagggtctt ccgcgccgtc gccgacgagg ggatcggcgt cgacatgatc
      361 gtgcagaacg ccgtacggga cggacggacc gacatctcgt tcacggtctc gaaggaggac
      421 ctcgcgcacg cgcagagcgt catcgagagc atcgtggacg agatcggcgc acagcggttc
      481 acggccgacg ccggcatcgc gaaggtctcg ctggtgggtg cggggatgaa gacgcacccc
      541 ggcgtccagg ccgagatgtt cgcggcgctc gccgaggccg gcgtcgagga catcgagatg
      601 atctcgacgt cgtcggtccg gatcacgtgc ctgatcccgg ctgcgaagat cgagtcggcg
      661 gtccgcacgc tccacgagcg gttctcgcta tcggcggagt cggcgatccg ggagcactag
      721 tgctcgtctg tcccacctgc cgcagcgaca accaggaaga cgcgcagttc tgcacgacgt
      781 gcggacggtc gctctctccc gacgaggccg cgctcgtcgc gccggcccgc cgagagcccg
      841 ccgatctcga gatcgacatc ccgccgcgga agccgcagag cccgcttccg ggcctgttcg
      901 cgctcgccgc cgtgctcgtc gcggggctcg gcgccgtgac gtggttcgcg cttcgtccga
      961 acccctgtgc cgggaagttc tcgagcgagc gcttcccgta ctgcatggcc gtcccgcggg
     1021 gctggcagga ggcgcaggtc gacgtgcagg ggacggccgt ggacgcgttc acgccttcgg
     1081 acgacgggcc gacggtgttc gtctacgccg cggaggcgcc ggacgggctc gacaccgagg
     1141 ggtacacgga cgacctcagg agcgaggcgc aggactctgg cctgttcccg acgaacccgc
     1201 gtccgatcga gatcggcgga gacgatgccc tcgtgtggga gctgacgaag agcgacacga
     1261 cgacgggctc gatgatcacc gaacggcgcg tgacgctggt ccgcgacggg acgctgtggc
     1321 agatcctgct gttcggaaac gaacgcgccg tcaaccgctc ggagccgcag ttcgagcgca
     1381 tgctcgagac ctgggcctgg cagtagaacc cggggtcggc gccacgaccg ccggtagact
     1441 gggtccgatg cgagtcgcga tcctcggagc gaccggcgcc gtcggtcgcg agatgctgcg
     1501 gacgctcgag gaacggtcgt tccccctcga cgccctcgtg ctgctgtcgt cgcctcgatc
     1561 cgccggacgc cggctgccgt tccgcggcga cgacgtcgtc gtgcgagcgg tggaggacga
     1621 ctcgttcgag ggtgtggacc tcgcgctgtt ctccgccggc gcgacggcgt cgcaggagta
     1681 cgcgccccgc gcggtggacg acggggccgt cgtcgtcgac aactcctcgg cgttccgcat
     1741 ggacccgggc gtgccgctcg tcatccccga ggtcaacgcg gaggcgctct ccgcgcaccg
     1801 cgggatcgtc gcgaacccga actgcacggc gatcacggcg atcatggcgg tggcgccgct
     1861 ccatcgcgcg gcgggactgc gctcgctcgt cgtctcgtcg taccagtcgg tgtcgggcgc
     1921 cggacacaag ggcgtcgcgg agctcctcga gcaggtcgac aagctgcgcg gcgacgagga
     1981 ggatctcgcg cgccccgacc ccgccgtact tccggtcggg gaggtcttcg ggaagacgat
     2041 cgcgttcaac gtcatccccc ggggcgggag cttcgagccc gacggttgga ccaccgagga
     2101 gacgaagctc gtcaccgaga cgaggaagat cctggagctg ccggagctcg cggtggcggc
     2161 gacggtcgtg cgcgtcccgg tcgtcgtcgg ccactccgtg tcggcggtcg cgacgttcga
     2221 tcgtgcgatg acgccggacg aggctcgcgg tgtgttcgcg cgagcgcctg gcatgctcgt
     2281 cgtggacgac ccggcgtccg acacgttccc cacgccgctc gctgccgcgg gacgcgacga
     2341 ggttctcgtc ggccgcatac gatccacgca agggatcgcg aacgcggtcc agctgttcgc
     2401 cgcctcggac aacctccgca agggcgcagc gctgaacgcc gtccagatcg cggagctcgt
     2461 cgccgcgcgc tagcgcgctt cgcggcgact ggtgagatag gcgccgagga gcacgagcgc
     2521 cacgccggcg agcgagatcg gagccggccg ctccccgagg atcgtgacgc cgagcgccag
     2581 cgccaccacc ggcacgaagt agatcgctac cgctccgcgg gtcgccccgg cacgaccgac
     2641 gagcgtcgcc atcgcgatga acgcgagccc tgatccgaag acgccgagcg cgaccatcgc
     2701 ggcgagcgca tccagcgcga agctcgcgcg cggcagaccg aacgccagcc cgagcggcgc
     2761 gagcaggacg agcgccacga gctgcgcgcg gaacaccacc ggaagcgctc cgtacttctg
     2821 ctggagcggg accgtgatgt tcgcggagag gccgtagagc gtcacggcga ggatcaggag
     2881 cgcggctccg accgcggtcg ccgcggcgcc ctgaagtgac ggcagcagga tcagcacgac
     2941 gccggcgaag ccgagcgcga gccccacacc ctgtcgtgcc gccggagcgc gagcgagaag
     3001 gatccacgcg aacagcgccg cgaacagcgg catcgcgccg ttgatcatcc cggccaccga
     3061 cgatgcgatc ccgaggtcct ggccgatcgg gaacagcagc agcgggaccg ccatccagat
     3121 gacccccagc agggcgacgc gcgggaggtc ctcgcggtcg atcggacggc gcgcccgtcg
     3181 gaacgccccg accgcgaccg ctccgaacgc cagccgcagc agcgcgatca gcgtcggggg
     3241 gaacgcctcg agccccacgg cgatcagcag gtacgacgag ccccacatcg ccgcgatcgc
     3301 ggcgagcagg ccccactcgg tcgcgccgaa cgcctcggtg cgcgtcccgc gcgcggtcgt
     3361 gatgacaccg gtggccctca tgtcggtcga acgttcaacg cggacggggc ctgaggtatt
     3421 tctctccggt cctcgctacg gtacccgcgt gagatccgtc gactacgtca tcctctacgt
     3481 cgaggagctc gagcggtcga tcgccttcta ccgcgacgtg atcgggcttt cgctcaggct
     3541 cgagggcgac ggctacgcgg agttcgcgac ggaggggacg aagttcgggc tgttcgagcg
     3601 gtcgaagctc cccgagctca tcggtcgggc agccgctccc cgcgggccgg cggccgaggt
     3661 cgtgttcgtg gtcgacgacg tcgatgcgga ggccgagcgg ctcgccgccg ccggggcgcg
     3721 agtcctcatg ccgccgacgg atcgggcgtg gggtcacaga acgctccacg tctccgatcc
     3781 cgacgggcac gtcgtcgagc tcgcgcagga gatccctagg tcgtcttgac cagcagcacg
     3841 tggcagggag cctcgtgggc gacgcggctg ggcacgctcg cgaggaaccg ccgtagcccc
     3901 gtcatcccgc ggttcccgat cacgatcaga tcgcagtcgc gctcggccgc cacgtccccg
     3961 atcgtcctcg ccgggtcacc ctcgaccgtg agggcctcga ccgtcaggtc cggctgcagc
     4021 tccgcggccg tgcgctccgc gacgctcgta tccgcgccga cgtggagcag gaccgtgtcg
     4081 gcgcccacca cggacgcgag gcgcgccccg acggcgacgg ctcggcccgc cgtaggggag
     4141 ccgtcggtcg tgagcaggac gcggccgtac gtccgttgtc ggcggcctcg ggtcgtctgc
     4201 gcgatcagca catcgcacgg cgctcggtgc gctatgcggt tcgggacgtt gccgatccgg
     4261 gccggccgcc gtcccatccc gcggttgccg acgacgatga gatcggctcc ctcctgtcgc
     4321 gcgacgtcga ggagcacgtc ggcagggtcg ccctcgcgga gaaccgcgcg ggcgcgcgac
     4381 gtcgggacat gacgcccagc cacgtcgcga aggaggctgg ccccgatctc tcgcgtgccg
     4441 acgggggagt cgagcgagcc cgtcccgccg tacgcgtgga ccactgcgag ctcggcgccg
     4501 gtcgcctcgg cgagccgcgc ggcgtgcgcg acggccgacg atgccgaatc agatccgtcg
     4561 gttccgaccg cgaccagcga gagcacgggc gcatcatccc agaaggaagg gggccgcccc
     4621 ttg
//
LOCUS       DASZOB010000523         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48924, whole genome shotgun sequence.
ACCESSION   DASZOB010000523 DASZOB010000000
VERSION     DASZOB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48924"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..572)
                     /locus_tag="VG709_08215"
     CDS             complement(84..572)
                     /locus_tag="VG709_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33096.1"
                     /translation="MRKREPYMAYDQMDFNVPVYRNGDVYDRYKVRMNEMRESVRIIQ
                     QCLDGMPEGPFISDDRKVVLPPRNELHTSMESLIHHFKLVTEGYRVPEGEVYVPVESP
                     RGELGCYLVSDGGPRPWRVKFRAPSYVALEATAACMRDALIADLIAIVGSLDNVMGEV
                     DR"
     gene            612..1100
                     /locus_tag="VG709_08220"
     CDS             612..1100
                     /locus_tag="VG709_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33097.1"
                     /translation="MRKNEPYLSYDELDFNVPVYFNGDVYDRYKVRMNEMRESVRIIQ
                     QCLDGMPEGPWIADDRKVVLPPRHALHTSMDSLIHHFKLVTEGYRVPEGEVYVPIESP
                     RGELGCYLVSDGGPKPWRVKFRAPSYAALEATATCMTDALVADLIAIVGSLDNVMGEV
                     DR"
     gene            1100..1573
                     /locus_tag="VG709_08225"
     CDS             1100..1573
                     /locus_tag="VG709_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWC33098.1"
                     /translation="MPETELYQEIQRIADEYPVRRSAIMPALHLAQKQYGWLPPEALR
                     EVADALELTPAYCLAVASFYDMYHLEPVGRHMVEVCTNISCALVGAQQVLEAFEEELG
                     VRAGETTEDGQVTLRTIECLGGCGWGTVVAVDHRYREPVRPEDVPGIVAELRNGG"
     gene            1563..>1890
                     /locus_tag="VG709_08230"
     CDS             1563..>1890
                     /locus_tag="VG709_08230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195720.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HWC33099.1"
                     /translation="MAVSGTRLLVLENAYERDLTKLAEYERAGGYKSLRKAFATEQAK
                     LVEELLASGLRGRGGAGFPTGRKASFLPKPGQTPKPIYLTVNADESEPGTFKDREIMF
                     RNPHALI"
BASE COUNT          300 a          651 c          651 g          288 t
ORIGIN      
        1 gagcgcgccc atgatcgcgg agcggcgcac ggggtactgg tcggcgagct tctggatgtc
       61 ctcgtagagc tgtgtgttcg gcactagcgg tcgacctcgc ccatgacgtt gtcgagcgag
      121 ccgacgatcg cgatcaggtc agcgatgagc gcgtcgcgca tgcaggccgc ggtcgcctcg
      181 agcgcgacgt aggagggcgc gcggaacttg acccgccacg gccgcgggcc gccgtccgag
      241 acgaggtagc agccgagctc gccccgcggc gactcgacgg ggacgtacac ctcgccctcg
      301 ggcacgcggt agccctcggt gacgagcttg aagtggtgga tcaggctctc catcgaggtg
      361 tgcagctcgt tccgcggcgg gagcacgacc ttgcggtcgt cggagatgaa cggcccctcg
      421 ggcatcccgt cgaggcactg ctggatgatg cgcaccgact cgcgcatctc gttcatccgc
      481 accttgtacc ggtcgtacac gtcgccgttc cggtagacgg gcacgttgaa gtccatctgg
      541 tcgtaggcca tgtacggctc gcgcttgcgc agtcggggcc cgttctgcgc gcgtccggcg
      601 tcgactacgc catccgcaag aacgagccgt acctctcgta cgacgagctc gacttcaacg
      661 tccccgtcta cttcaacggg gacgtctacg accgttacaa ggtgcggatg aacgagatgc
      721 gcgagtccgt gcggatcatc cagcagtgcc tcgacgggat gcccgagggg ccgtggatcg
      781 ccgacgaccg caaggtggtg ctcccgcccc ggcacgcgct ccacacgtcc atggacagcc
      841 tgatccacca cttcaagctc gtgaccgagg gctatcgcgt cccggagggg gaggtgtacg
      901 tgccgatcga gtctccgcgc ggcgagctcg gctgctacct cgtctcggac ggcggcccga
      961 agccctggcg cgtgaagttc cgcgcgccct cgtacgccgc cctcgaggcg accgcgacgt
     1021 gcatgacgga cgccctggtc gcggacctga tcgcgatcgt cggctcgctc gacaacgtga
     1081 tgggcgaggt cgaccgctga tgcccgagac cgagctctac caggagatcc agcggatcgc
     1141 cgacgaatac ccggttcgcc gctcggcgat catgcccgct ctccatctcg cgcagaagca
     1201 gtacggctgg ctcccgcccg aggcgctgcg cgaggtcgcc gacgcgctcg agctcacgcc
     1261 cgcctattgc ctcgccgtcg cgagcttcta cgacatgtac cacctcgagc cggtcggccg
     1321 gcacatggtc gaggtctgca cgaacatctc ctgcgcgctc gtgggagcac agcaggtgct
     1381 cgaggcgttc gaggaagagc tcggcgtgcg cgccggcgag acgacggagg acggccaggt
     1441 gacgctccgg acgatcgagt gcctcggcgg ctgcggctgg ggcacggtcg tcgccgtcga
     1501 ccaccgctac cgcgagcccg tgcggcccga ggacgtgccc gggatcgtcg ccgaactgcg
     1561 caatggcggt tagcggcacc cgcctgctcg tcctcgagaa cgcgtacgag cgcgacctca
     1621 cgaagctcgc ggagtacgag cgcgccggcg gctacaagtc gctgcgcaag gcgttcgcga
     1681 ccgagcaggc caagctcgtg gaggagctcc tcgcctcggg tctccgcggc cgcggcggtg
     1741 ccggattccc gacggggcgc aaggcgagct tcctgccgaa gccggggcag acgccgaagc
     1801 cgatctacct gaccgtgaac gcggacgagt ccgagccggg gacgttcaaa gaccgcgaga
     1861 tcatgttccg caacccgcac gcgctgatcg
//
LOCUS       DASZOB010000524         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191327, whole genome shotgun sequence.
ACCESSION   DASZOB010000524 DASZOB010000000
VERSION     DASZOB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191327"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VG709_08235"
     CDS             <1..466
                     /locus_tag="VG709_08235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWC33100.1"
                     /translation="SQHPGEARYTDSVATARLENDLRCTITGADGASVTTDMPRSVGG
                     RGQNPSPGWFWRAATAGCVATVTAMRAAREGVELSNLEVTVDSESDDRGILGMDDSVP
                     AGPLSMRIRVRIVAPEADADQLRELASWGSDHCPMCDGIKRAVPVTLEIETG"
     gene            509..1060
                     /locus_tag="VG709_08240"
     CDS             509..1060
                     /locus_tag="VG709_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWC33101.1"
                     /translation="MAAGRLDDARSVFVDGMGTASATSGVLSQLQGRIFALLYLRDRP
                     VSLDEIAEELQQSKSNISMNIRGLVDWHLVRRVPVPSSRKDHYEAVADLWRVTQEIME
                     RRFRWNIRQVIAACDETRRAAASGRAGSREAQRERAFIESRLEALRSFAAAVDAGIEA
                     FSRGEPMAPPPLQAIPIERRRQR"
     gene            1057..1617
                     /locus_tag="VG709_08245"
     CDS             1057..1617
                     /locus_tag="VG709_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33102.1"
                     /translation="MIDVRTTLLDEALPRFDVRSRHARRVAASPEAVWRAVEAYDPRR
                     DASLIVRSLFLARGFGIPVGTIREVLAASGFTIIAERPGSEIVAGTTGRFWALRERAN
                     MAAPADLDAFLRFGRPGWAKAAVSVRVEPIDDASTDIVTETRVWCVDDHARRRFAAYW
                     TVIGAFSGWIRHGVLRAVARKAEEER"
BASE COUNT          254 a          606 c          626 g          269 t
ORIGIN      
        1 gtcccagcat ccgggggaag ctcggtacac cgactcggtc gcgacggcgc gcctggagaa
       61 cgacctcagg tgcacgatca cgggcgccga cggtgcctcg gtcacgaccg acatgccgcg
      121 ctcggtcggc ggacgtggcc agaacccctc gccggggtgg ttctggcgag ccgccacggc
      181 tggctgcgtt gccaccgtca cggcgatgcg agcggcgcgg gagggcgtcg agctctcgaa
      241 cctcgaggtc acggtcgaca gcgagtccga tgaccgcggg atcctcggga tggacgattc
      301 cgttccggcc ggcccactct cgatgcggat ccgcgtgcgc atcgtcgcgc cggaagccga
      361 tgccgaccag ctccgcgagc tggcgtcgtg gggatcggat cactgcccga tgtgcgacgg
      421 gatcaagcgc gccgtccccg tgacgctgga gatcgagacg gggtagcgct tgacgcgaac
      481 gttcagaacg ttctaaacat acaccgagat ggctgctggc cggctcgacg acgcgaggtc
      541 cgtcttcgtg gacgggatgg ggaccgcctc ggccacgtcg ggcgtcctgt cgcagctgca
      601 gggacgcatc ttcgcgctgc tgtacctccg cgatcgcccc gtgtcgctcg acgagatcgc
      661 cgaagagctc cagcagtcga agagcaacat ctcgatgaac atccgagggc tcgtcgattg
      721 gcacctcgtc cggcgcgttc cggttccgag ctcgcggaag gaccattacg aggcggtggc
      781 agacctctgg cgcgtaaccc aggagatcat ggagcggcgg ttccgctgga acatccgtca
      841 ggtgatcgcg gcgtgcgacg agacccggcg cgccgcggca tccggccgag cgggctcgcg
      901 cgaggctcag cgcgagcgcg cgttcatcga gagcaggctc gaggcgctgc ggtcgttcgc
      961 ggcagcggtc gacgcaggca tcgaggcgtt ctcccgtgga gagccgatgg cgccgcctcc
     1021 gctccaggcg atcccgatcg agcgccgacg ccagcgatga tcgacgtccg aacgacgctc
     1081 ctcgacgagg cgcttccgcg gttcgacgtc cggagccgcc acgcccggcg cgtcgccgcc
     1141 tctcccgaag cggtctggcg ggcggtcgag gcttacgacc cgcgccgcga cgcgtcgctc
     1201 atcgtccgct cgctcttcct cgcccgagga ttcgggatcc ccgtagggac gatccgcgag
     1261 gtcctggccg catctggatt cacgatcatc gccgagcggc ccggttcgga gatcgtcgcc
     1321 ggaaccaccg gcaggttctg ggcgctccgc gaacgggcga acatggcggc gcccgccgac
     1381 ctcgacgcgt tcctccggtt cggccgaccc ggctgggcga aggctgccgt gtcggtgcgc
     1441 gtcgagccga tcgacgacgc gtccaccgac atcgtgaccg agacgcgcgt ctggtgcgtc
     1501 gacgaccacg cgcggcgtcg gttcgccgcc tactggacgg tgatcggcgc cttcagcggg
     1561 tggattcggc atggcgtcct ccgcgcggtg gcgcggaagg ccgaggaaga gcggtgaacg
     1621 tcccgttcgt cgtcgccggc gcgctcgcgg tgctcgccgc agcgatccac ggcaccggcg
     1681 gggagatctg gatccttcgg aggctgttct ccggcgacga cgaacgggac gttcaccgct
     1741 cttcctcggc cttcc
//
LOCUS       DASZOB010000525         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48985, whole genome shotgun sequence.
ACCESSION   DASZOB010000525 DASZOB010000000
VERSION     DASZOB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48985"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VG709_08250"
     CDS             complement(<1..1473)
                     /locus_tag="VG709_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWC33103.1"
                     /translation="MTVAELLVTAGGVALAAGLAWFFFGPKQGGRTTIRGGVQEIEVK
                     VKGGYSPSVVRARRGVPLRIVFDRQEGGECTSEVVFADFQVRRALPAFRRTELQLVPE
                     RSGEFGFACGMNMVHGRLVVEEDDGDGDVEISERDAASEDRETEDHVHPDAGSVAVDE
                     SSDGRVALALRGSVSACPTCLATIERALDETPGVRATRGDASRDRVVVEFDASSVTVR
                     DLEEVVAARGYRVERRSADASEDRGDEEAEARRGEISDLSRRVIVGALLTAPVVVAVM
                     GKEFLRADWVPEILMSPWLQLALVAPVMAYTGWPIHSVGWNTLRHRTAEMNTLITIGT
                     TAAFVYSLLVTLAPGLFPAEVRDVYYEAVGVILTLILLGRLLEAKARAGTGEAIRKLL
                     GLRPRTARVVRDGGEADVPIDEVGVRDEIVVRPGERVPVDGEIVEGRSTIDESMVTGE
                     SVPVDKEPGDHVVGATVNQTGSFRFRATAVGSETVLAQVIR"
     gene            complement(1737..2264)
                     /locus_tag="VG709_08255"
     CDS             complement(1737..2264)
                     /locus_tag="VG709_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWC33104.1"
                     /translation="MYLRFGAMILSATVVMYAVMYVNTYEWGHVQWSESRMFMAFTMG
                     GTMALVMLGWMLNMYRNARVNAAIVVVSVLLLAGVIYLDRSQTTVQDDSFMSAMIPHH
                     SIAILRAERAEIADVRVCELAVAISEAQRREILEMEWLISDIERNGPALTAAQAKARP
                     VPDFDGPALRECPTA"
     gene            complement(2359..2763)
                     /locus_tag="VG709_08260"
     CDS             complement(2359..2763)
                     /locus_tag="VG709_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWC33105.1"
                     /translation="MARAAEMLETHPWPGNVDRDVLARCIEECFSCAQTCTSCADACL
                     SEEMVADLRKCIRLNLDCADICDTTGRVLTRQTGYDAPTTKAQVQACLEICRTCAEEC
                     ERHAEMHEHCRICAEACRRCAEACERLLAAMK"
     gene            complement(2869..3147)
                     /locus_tag="VG709_08265"
     CDS             complement(2869..3147)
                     /locus_tag="VG709_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWC33106.1"
                     /translation="MGTGDDHDWTDKRKLGNRARRIEGQAAAVARMLDEGRSCREVLQ
                     QLAAFLSAGQELAVLVVEDHVLARSRAGADADALSAELASHLRRVLKR"
BASE COUNT          488 a         1196 c         1100 g          531 t
ORIGIN      
        1 gcggatgacc tgcgcgagga cggtctccga gccgacggcc gtcgcgcgga atcggaacga
       61 gcccgtctgg ttgaccgtgg ccccgacgac gtggtcgccg ggctccttgt cgaccgggac
      121 ggactcgccc gtcaccatcg actcgtcgat cgtggaccgc ccctcgacga tctcaccgtc
      181 gaccgggacc ctctcgccgg gacgtacgac gatctcgtcg cggacgccga cctcgtcgat
      241 cggcacgtcg gcctcgccgc cgtcccgcac gacgcgtgcg gtccgcggcc gcaggccgag
      301 cagctttcgg atcgcctctc ccgtcccggc gcgcgccttc gcctcgagca gccggccgag
      361 gaggatcagc gtcaggatga ccccgacggc ctcgtagtac acgtcgcgga cctccgcggg
      421 gaagaggccc ggcgccaacg tgacgagcag gctgtagacg aacgccgcgg tcgtcccgat
      481 cgtgatgagc gtgttcatct cggccgtccg gtggcggagc gtgttccagc cgaccgagtg
      541 gatcggccac cccgtgtagg ccatcacggg ggcgacgagg gcgagctgga gccacgggct
      601 catgaggatc tcgggcaccc agtcggcccg gaggaactcc ttgcccatca cggccacgac
      661 gaccggggcg gtgaggagcg cgccgacgat cacccgtcgc gacaggtcgc tgatctcgcc
      721 ccgccgcgct tcggcttcct cgtcgccccg gtcctccgac gcgtcggcgg aacggcgctc
      781 gacgcgatat ccgcgagcgg ccacgacctc ctccagatct cgcaccgtca ccgacgacgc
      841 gtcgaactcg acgacgacgc ggtcgcggga cgcgtcgcca cgggtcgcgc ggacgcccgg
      901 cgtctcgtcg agcgcgcgct cgatcgtcgc gaggcaggtc gggcatgccg acacgcttcc
      961 gcgcagcgcg agcgcaacgc ggccgtccga cgactcgtcg accgcgacgg accccgcgtc
     1021 ggggtggaca tggtcctcgg tctcgcggtc ctcgctcgcc gcgtcgcgct cggagatctc
     1081 cacgtctccg tcgccatcgt cctcctcgac gacgagccgg ccgtggacca tgttcatccc
     1141 gcacgcgaac ccgaactcgc cgctgcgctc gggcacgagc tggagctccg tgcggcggaa
     1201 cgccggaagg gcgcgccgaa cctggaagtc ggcgaacacc acctcggagg tgcattcgcc
     1261 gccctcctgt cggtcgaaga cgatgcggag cggcacgccg cgccgggccc tgacgacgct
     1321 ggggctgtat ccgcccttga ccttgacttc gatctcctgc acgccgcctc ggatcgtcgt
     1381 ccggccgccc tgcttaggcc cgaagaagaa ccacgcaaga ccggccgcga gcgcgacgcc
     1441 gccggcggtg accagtagct ccgcgaccgt caacccgtcc tcctcgtccg gcgtctcgta
     1501 gagaacctcc cggtctcatc gtatacccca ggggggtatc tggggaggct ccatccaagg
     1561 cgtgcgtcgt ccggagcgaa ctctcacttc atcgcggggg gcagccgctc gcacgcgtcg
     1621 gcgcagcgcg ggcacacctc cgcgcggatc cagcaaatgc tcgtgcatct cggcgtggcg
     1681 catccgcgca tcgccctttg cagcatcgcc ccggcgacgg tgcgtccggc ggggattcaa
     1741 gcggttggac actcgcggag ggccggtccg tcgaagtcgg gaacggggcg ggccttcgcc
     1801 tgcgcggcgg tgagggcggg accgtttcgc tcgatgtcgc tgatgagcca ttccatctcg
     1861 aggatctcgc gacgctgcgc ctcgctgatc gcgacagcca gctcgcagac cctgacgtcg
     1921 gcgatctcgg cacgttcggc cctgaggatg gcgatggagt ggtgcgggat catcgcgctc
     1981 atgaaggagt cgtcctggac ggtcgtctgg ctgcgatcca gatagatcac gcctgcaagg
     2041 aggaggacgc tcacgaccac gatggcggcg ttcactctgg cgttccggta catgttgagc
     2101 atccacccca acatgacgag ggccatggtg cctcccatgg tgaacgccat gaacatccgg
     2161 ctctcgctcc actgaacgtg accccattcg taggtgttga cgtacatgac cgcgtacatc
     2221 accaccgtcg cgctgaggat catcgccccg aaccggagat acatcttcgg ctcgcgtcgc
     2281 ttgtcgtgtt gttggtcctg gtgctggtcg gcttcatcgg ccgccgcgtg gcttggccgc
     2341 gtcattcgcg ctccgtcgct acttcatagc ggcgaggaga cgctcgcacg cctccgcgca
     2401 gcgccggcac gcctccgcgc agatgcggca gtgctcgtgc atctccgcgt gccgctcgca
     2461 ctcctcggcg caggtccggc agatctcgag gcacgcctgc acctgggcct tcgtggtcgg
     2521 ggcgtcgtag ccggtctggc gcgtcagcac ccgcccggtc gtgtcgcaga tgtcggcgca
     2581 gtcgaggttg agccggatgc acttccgtag gtccgccacc atctcctcgg agagacatgc
     2641 gtccgcgcag ctcgtgcacg tctgtgcgca cgagaagcac tcctcgatgc accgcgccag
     2701 cacgtcccgg tcgacgttcc ccggccacgg gtgggtctcg agcatctcgg ctgctcgagc
     2761 catcggtcgc ctccttcctt ccgtcgctag ccggtcgctt acccatactc ggggggagta
     2821 ttcgctggcg acacgacgtc ctgccatgcc gccgcgagga ggggcgttct agcgcttcag
     2881 cacccgcctc aggtgggagg cgagttccgc cgacagcgcg tccgcatccg cgccggcacg
     2941 tgaacgtgcg agcacgtggt cctcgaccac gagcacggca agctcctgtc cggcggagag
     3001 gaacgccgcg agctgctgca gaacctcgcg gcacgaacgg ccctcgtcga gcatccgggc
     3061 caccgcagcg gcttggccct cgatgcgccg tgccctgtta ccgagcttcc gcttgtcggt
     3121 ccagtcgtgg tcatcaccgg ttcccatgac acgtactttg ccccggcgtg caacagccga
     3181 taccgccgca cgtgtgccag atcaagcggc tgcgatgagg gacggaggtt cggacaccgc
     3241 agggatccgg tcctcgcgtt cagcgtgagc cagccgtcat gcctccagcg agcgaacaaa
     3301 agggaagggg cccgc
//
LOCUS       DASZOB010000526         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_180530, whole genome shotgun sequence.
ACCESSION   DASZOB010000526 DASZOB010000000
VERSION     DASZOB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180530"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VG709_08270"
     CDS             <1..553
                     /locus_tag="VG709_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWC33107.1"
                     /translation="EGIELTVAVNLFGRDLLDLGLPHEVEELLARWRIPADQLELEIA
                     ENTILTEPERARTILERLGESGVTLAIDDFGSGYTSFHYLKRLPVDVLKIDRSFVQAM
                     NASGRDAIIVRSTVELGQNLGLVVVAEGVESEEIWNRLAASGCDRVQGYFLSRPMPAS
                     GVAPWIRAREQQRSLPPPHAAAI"
     gene            complement(559..885)
                     /locus_tag="VG709_08275"
     CDS             complement(559..885)
                     /locus_tag="VG709_08275"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWC33108.1"
                     /translation="MSVWYGVRDLERARAFYTETLGFEELYRDADGRWMRLGRNGAEL
                     ALAESDEGTGAVLAVAVEDVKAEAARLREAGVEVGVVLEIHGVLRLLDVFDPEGNRLQ
                     LTQDLA"
     gene            complement(882..>1903)
                     /locus_tag="VG709_08280"
     CDS             complement(882..>1903)
                     /locus_tag="VG709_08280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWC33109.1"
                     /translation="TETPGRVQPDEGGAEVGVEKPAEGPAREDERERGREAGAEEGEP
                     VDPEMARAAERRDGGRVKASPLARRLAKERGIELASLQGTGPEGRIVAEDGERAVAQE
                     PAPATAEAPAGEYEAVQLTQMRRTIARRMTEAWETPHFQIAMSADMRAAIRVREALVA
                     RVEEGATKPTFSDVLTKACAVSLMRHREMNAHYAGDEVRLFPTADIGMAVAIPQGLVV
                     PVIRGCERKTITEIAAARADLVERTRSGKLQQADLEGGTFTISNLGMYGVERFVAVLN
                     PPQAGILAVGAIEERAVVEGGEIVARPMMEMTLSCDHRSVDGATASEFLRSVKEFLEE
                     PALML"
BASE COUNT          256 a          710 c          643 g          294 t
ORIGIN      
        1 cgaggggatc gagctcaccg tcgccgtcaa tctcttcggg cgggatcttc tcgatctggg
       61 ccttccgcac gaggtggagg agctccttgc acgttggcgg atcccggccg accagctgga
      121 gctcgagatc gccgagaaca cgatcctcac cgagccggag cgcgcgcgga cgatcctcga
      181 gcgcctcggc gagagcggcg tcacgctcgc gatcgacgac ttcgggagcg ggtacacgtc
      241 gttccactac ctcaagcgtc tcccggtcga cgtgctcaag atcgaccgct cgttcgtcca
      301 ggcgatgaac gcgagcggac gcgacgccat catcgtccgc tcgaccgtcg agctcggcca
      361 gaacctcggg ctcgtcgtcg tggccgaagg cgtcgagagc gaggagatct ggaaccggct
      421 cgccgcgagt ggctgcgaca gggtccaggg ctacttcctc agccggccga tgcccgcgtc
      481 cggcgtcgca ccgtggattc gcgcacgcga gcagcagcgc tcgctcccgc cgccgcacgc
      541 ggcagcgatc tgaatgagct aggccagatc ctgcgtcagc tggaggcggt tgccctccgg
      601 gtcgaagacg tcgaggaggc ggagcacgcc gtggatctcg aggacgacgc cgacctcgac
      661 gcccgcttcg cgcaggcgcg ccgcttccgc cttcacgtcc tcgaccgcga cggcgagcac
      721 ggcgcccgtg ccctcgtcgc tctcggcgag ggctagctcg gcgccgtttc ggcccaggcg
      781 catccagcgg ccgtcggcgt cgcggtagag ctcctcgaag ccgagcgtct ccgtgtagaa
      841 cgcgcgcgcc cgctcgaggt ctcgaactcc gtaccagacg ctcacaacat gagggcgggc
      901 tcctccagaa actccttcac ggagcggagg aactcggacg ccgtcgcgcc gtcgacggag
      961 cggtggtcgc aggagagcgt catctccatc atcggacggg cgacgatctc gccgccttcc
     1021 acgacggcac gctcctcgat cgcgccgacc gcgaggatgc cggcctgcgg cgggttgagg
     1081 acggccacga agcgctcgac gccgtacatc ccgagattcg agatcgtgaa cgttccgccc
     1141 tcgaggtccg cctgctggag cttcccggag cgggtgcgct cgacgaggtc cgcgcgggcg
     1201 gccgcgatct cggtgatggt cttcctctcg cagccgcgga tcaccgggac gaccaagccc
     1261 tgcgggatcg cgaccgccat cccgatgtcc gcggtcggaa acagtcgaac ttcgtcgccc
     1321 gcgtagtgcg cgttcatctc gcgatggcgc atgagcgaaa ccgcgcacgc cttcgtgagc
     1381 acgtccgaga acgtcggctt cgtcgcgccc tcttcgacgc gcgcgacgag cgcctcgcgc
     1441 acgcggatcg cggcgcgcat gtcggccgac atcgcgatct ggaagtgcgg cgtctcccac
     1501 gcctccgtca tgcggcgcgc gatcgtccgg cgcatctgcg tgagctgcac ggcctcgtac
     1561 tcgccggccg gcgcctcggc cgtagccgga gcgggctcct gtgcgacggc tcgctcgccg
     1621 tcctcggcaa cgatccggcc ctcagggccg gtgccctgca ggctcgccag ctcgatcccc
     1681 cgctccttgg cgagccggcg tgcgagcggc gaggccttta cgcgcccgcc gtctcggcgc
     1741 tcggcggccc gcgccatctc ggggtcgacc ggctcgcctt cttcggcgcc cgcctcgcgg
     1801 ccgcgctctc gttcgtcctc ccgagcgggg ccctcggccg gcttctcgac gccgacctcc
     1861 gcgcctccct cgtccggctg cacgcggccg ggcgtctcgg tct
//
LOCUS       DASZOB010000527         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_48994, whole genome shotgun sequence.
ACCESSION   DASZOB010000527 DASZOB010000000
VERSION     DASZOB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48994"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VG709_08285"
     CDS             <1..1234
                     /locus_tag="VG709_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWC33110.1"
                     /translation="ISRYFPEFETEIVRRADAVGRTEALGPLHGVPVAVKDLFDVAGW
                     PTTGCSEAYARRVATADAEVVRRLREAGAVVIGKTNQHELAAGATNLVSACGPVRNPW
                     DPGRIAGGSSGGSAAVVAARVVPLALGTDTGGSIRIPASLCGTAGLKPTWGAVPMEGA
                     MPLAAALDSAGPLATTVEDIALAFSVLTGASLRLSDRLDGLRVAAVRLHTVPLRRDVA
                     AACDALVDVLVAAGASRVEVASLDYEPELWSRIGWREFADAHGHLLEWPERLGRPTRE
                     FLDEGRSRSDDDYRDALEEASALRRGFLDRLRDADVLVAATTAFPAPAIDEPIVDVDG
                     HELDVRKGAVSVLTRPVNLIGFPALALVSGFDADGLPLGAQLIGRAGEEELLLRAGLA
                     FQRATAHHRRAPGFPATA"
     gene            complement(1224..1568)
                     /locus_tag="VG709_08290"
     CDS             complement(1224..1568)
                     /locus_tag="VG709_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33111.1"
                     /translation="MAREVIYETLWTKTPGQERLRKSAEGRVKALLRDGWHEMGRETV
                     GPDTVRIRFERELANRPLPPLRRVPEPPPRRTGDRGRGGDRGRRTGSPGPAARGVAGG
                     GPGGAARGAPRR"
BASE COUNT          182 a          646 c          638 g          248 t
ORIGIN      
        1 gatctcgcgc tacttccccg agttcgagac cgagatcgtc cgccgcgccg acgcggtcgg
       61 ccgcacggag gcgctcgggc cgctccacgg tgtcccggtc gccgtcaagg accttttcga
      121 cgtggccggc tggccgacga ccgggtgctc cgaggcgtac gcgaggcgcg tcgcgacggc
      181 cgacgccgag gtcgtccgca gactccggga ggccggcgcg gtggtgatcg ggaagaccaa
      241 ccagcacgag ctcgcggccg gcgcgacgaa cctcgtttcc gcgtgcggtc cggtccggaa
      301 cccttgggac cccggtcgga tcgccggcgg ctcgagcggc ggctcggccg cggtcgtggc
      361 ggcgcgcgtc gtcccgctcg cccttgggac cgacaccgga gggtcgatcc gcatccccgc
      421 gtcgctctgc gggacggccg ggctgaagcc gacgtgggga gccgtcccga tggagggcgc
      481 gatgccgctc gccgccgcgc tcgacagcgc cgggccgctg gcgacgacgg tcgaggacat
      541 cgccctcgcg ttctcggtgc tcacgggagc gtcgctccgg ctctcggacc ggctcgacgg
      601 cttacgcgtc gcggcggtgc gactccatac ggtcccgctc cgccgcgacg tcgctgcggc
      661 gtgcgatgcg ctggtcgacg tcctggtcgc cgcgggggcg tcgcgcgtcg aggtcgcgtc
      721 gctggactac gagcccgagc tctggtcgcg gatcggttgg cgcgagttcg cggacgcgca
      781 cggccacctc ctcgagtggc ccgagcggct cggccgcccg acgcgcgagt tcctcgatga
      841 gggccggagc cgcagcgacg acgactaccg cgacgcgctc gaggaagcgt cggccctgcg
      901 gcgggggttc ctcgaccgcc tgcgagatgc cgacgtgctc gtcgcggcga cgactgcctt
      961 cccggcgccc gcgatcgacg agccgatcgt cgacgtcgac gggcacgagc tcgacgtacg
     1021 gaagggcgcg gtctcggtcc tcacgcgtcc cgtgaacctc atcgggttcc cggcgctcgc
     1081 gctcgtgtcc gggttcgacg ccgatgggtt gccattggga gcgcagctca tagggcgcgc
     1141 cggcgaggag gagcttctgc ttcgagccgg cctcgcgttc cagcgcgcca ccgcccacca
     1201 ccggcgggcg ccggggttcc cggctaccgc ctaggggccc cgcgtgcagc gccgcccggc
     1261 ccgccgcccg cgacgccgcg cgccgccgga cccggggatc ccgtcctgcg gccgcggtcg
     1321 ccgccgcgcc cgcggtcgcc ggtccttcgc ggcggcggct cggggacgcg acgcagcggc
     1381 ggaagcggac ggttcgcgag ctcgcgctcg aagcggatcc gcacggtgtc gggcccgacc
     1441 gtctcgcgcc ccatctcatg ccatccgtct cggagcagcg ccttcacgcg tccctcggcg
     1501 gacttccgga gcctctcctg ccccggggtc ttcgtccaga gcgtctcgta gatgacctct
     1561 cgcgccatgc ttcctccacg ggccgtcggt cgacccggcg ggtcactgta gcaggacgag
     1621 cgtcgttcgc cgctttctct gcgacgatgt gccgccatgt cggaggcgcg gcgcgatccg
     1681 gtcggcgggg cgctcgtcgg cgtcgcgtcg ttgc
//
LOCUS       DASZOB010000528         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_140505, whole genome shotgun sequence.
ACCESSION   DASZOB010000528 DASZOB010000000
VERSION     DASZOB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140505"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..561)
                     /locus_tag="VG709_08295"
     CDS             complement(55..561)
                     /locus_tag="VG709_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33112.1"
                     /translation="MRLVTKVAVVLVGVLVLGGPAAAQQYPPDEPTCGISDTVVVPGQ
                     AITITCDGWQPGSEVTATFFSHPVVLGSDTANSQGEVSITGTIPGDAEPGDHVIRVEG
                     IADDGEPAVVEIGVTVAGAGAAAGDGAAQVAVTGRNVTVGFMILAGLIVGGTVALLIG
                     RRRASTTR"
     gene            complement(700..>1924)
                     /locus_tag="VG709_08300"
     CDS             complement(700..>1924)
                     /locus_tag="VG709_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HWC33113.1"
                     /translation="TARGGDMTLSGFSAIATLRDVPAGRVEPPNADYADRFNRYGGAG
                     YWRNINMTADFPSAATAIERLYEEVTGTAVDGTIAADPFALEAMLGVVGEVRVPRIGE
                     TVDAGSVVPFVTNEAYSRFRSPAVRKRLLGDVAGTVFERFLASPGGPLGFRALADAAA
                     AGHLLMHSTDPREQRGLETAGVSGALGPATGDYLAVVGNNAAGNKADFYADRAIRYEV
                     RLGAEGTGVGHATVEITNDAPKQGEPRYVIGPYSGEFDAGENVTLLDLYCGRECGVEE
                     FRIDGGRATVGSEEELGHRVYPVRISIPSGATRVLDYGYQVPTAWAGDDVAGTYTLTF
                     RGQTTIRPAALVVDVRAPEGMEIVAASPGMEWSGGRAVWRGSAPDLETFEVRFERPPL
                     ERIWHFLSRPVFRLG"
BASE COUNT          279 a          723 c          647 g          275 t
ORIGIN      
        1 atcctacagg accccccgcc gtgaaaagcc gcgtatggga cggcgcgggt gccgctaccg
       61 ggtggtgctc gcgcgccggc gtccgatgag gagcgccact gtcccgccga cgatgaggcc
      121 ggccaggatc atgaagccga ccgtgacgtt gcgaccagtg accgccacct gggccgcgcc
      181 gtcgccagcc gctgcgcccg cgccggcgac cgtgacgccg atctccacga ccgcgggctc
      241 gccgtcgtcg gcgatgccct ccacccggat cacgtggtcg ccgggctccg cgtcaccggg
      301 gatcgtcccg gtgatcgaga cctcgccttg ggagttcgcc gtgtcggagc cgaggacgac
      361 cgggtgcgag aagaacgtcg cggtcacctc ggagcccggc tgccagccgt cgcaggtgat
      421 cgtgatcgcc tgaccgggca cgacgacggt gtcggagatg ccacaggtcg gctcgtccgg
      481 cgggtactgc tgcgcggcgg cgggccctcc caacacgagg acgccgacca gcaccacagc
      541 aactttcgtg acaagcctca tgaggcgatc tcctccctac ccacgttgag cattgcggtc
      601 gatctgatcg agacgtttcc caggataggg tttctcagtc ctcgtggcaa gtaccggacg
      661 acccatcgca attgcgcaga aatccgtcgg gcgaacggcc tacccaaggc gaaataccgg
      721 cctactgagg aagtgccaga tccgctcgag cggcgggcgc tcgaagcgga cctcgaacgt
      781 ctcgaggtcg ggtgcgctcc cgcgccagac ggcccggccg ccgctccact ccatccccgg
      841 gctggcggcg acgatctcca tcccctccgg ggcgcggacg tcgacgacga gggccgccgg
      901 gcggatcgtc gtctgccccc ggaacgtcag cgtgtacgtg ccggccacgt cgtcgccggc
      961 ccacgccgtc ggcacctggt acccgtagtc gaggaccctc gtagcgccgc tcgggatcga
     1021 gatccggacc gggtacacgc ggtgcccgag ctcctcctcg gaccccacgg tggcgcgccc
     1081 gccgtcgatg cggaactcct cgacgccgca ttcacgtccg cagtagagat cgagcagcgt
     1141 cacgttctcg ccggcgtcga actcaccgga gtacggcccg atcacgtacc gcggctcgcc
     1201 ttgcttgggg gcgtcgttcg tgatctcgac ggtcgcgtgt ccgaccccgg tcccttccgc
     1261 cccgagccgg acctcgtagc ggatcgcgcg atcggcgtag aagtcggcct tgttccctgc
     1321 ggcgttgttg ccgaccaccg cgaggtagtc gccggtcgcc ggcccgaggg ccccgctcac
     1381 ccccgcggtc tcgaggcccc gctgctcgcg aggatccgtc gagtgcatga ggaggtgtcc
     1441 ggccgccgcc gcgtccgcga gcgcgcggaa cccgagcggg ccgccgggag acgcgaggaa
     1501 ccgttcgaag accgtccccg cgacgtcgcc cagcaaccgc ttccggaccg cggggctccg
     1561 gaaccgcgag tacgcctcgt tcgtcacgaa cggcacgacg gagccggcgt cgacggtctc
     1621 gccgatccgc ggcacgcgca cctcgccgac gaccccgagc atcgcctcga gcgcgaacgg
     1681 gtcggccgcg atcgtcccgt cgaccgccgt ccccgtcacc tcctcgtaca ggcgctcgat
     1741 cgcggtcgcg gccgacggga agtcagccgt catgttgatg ttccgccagt acccggcacc
     1801 cccgtagcgg ttgaaccggt ccgcgtaatc ggcgttcggc ggctcgaccc tccccgcggg
     1861 gacgtcgcgc agcgtggcga tcgccgagaa ccccgacagc gtcatgtcgc cgccgcgcgc
     1921 ggtc
//
LOCUS       DASZOB010000529         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_18433, whole genome shotgun sequence.
ACCESSION   DASZOB010000529 DASZOB010000000
VERSION     DASZOB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18433"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..399)
                     /locus_tag="VG709_08305"
     CDS             complement(232..399)
                     /locus_tag="VG709_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33114.1"
                     /translation="MTHHDMSHSHAPEPAPARDPASGWTGYAAVKYGFIFLIVVAILW
                     FLARYVLGIFE"
     gene            complement(462..1241)
                     /locus_tag="VG709_08310"
     CDS             complement(462..1241)
                     /locus_tag="VG709_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HWC33115.1"
                     /translation="MTADATNHVAAEAQTDAELQDKRSDELFRALPDDAAREQLVEMY
                     KPLAEYLARRFYGRGEPLDDLIQVASIGLIKAIDRFDVDRGVKFSTYATATIVGELKR
                     HFRDKGWALRVPRRLQESGLKVGRAVTDLYQDLGRAPTVREIGKRTDLSDEEVLEAME
                     TAHAYSTASLDAPTDEEGATSIQKLGAEDDTYELLESWTSVAPVLRELPYRERRILYL
                     RFFRGLTQTQIADELGISQMHVSRLLSRTLRQLRDAVGEEV"
     gene            complement(1238..1615)
                     /locus_tag="VG709_08315"
     CDS             complement(1238..1615)
                     /locus_tag="VG709_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC33116.1"
                     /translation="MSGDLTVTVPARPEYVQVLRSVVAGMAARLDFPYDAIEDLKIAV
                     DEACAQLLPLRPGSLSMRVADGRGEIEATVSADVRAAKWPPDEAERSMAWQVLRALTD
                     EARWERSGDGPALHLVKRIGAPA"
     gene            1667..2011
                     /locus_tag="VG709_08320"
     CDS             1667..2011
                     /locus_tag="VG709_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWC33117.1"
                     /translation="MAEINLVVEANERGPWTVLALEGEIDLYTAPKLKEQISELVATD
                     RAQLVVDLSRVEFLDSTGLGVLIGGLKRAREKGGELALAGATEPVRKVLSITGLDKVF
                     PLHDDVDVATGA"
     gene            2016..2438
                     /locus_tag="VG709_08325"
     CDS             2016..2438
                     /locus_tag="VG709_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC33118.1"
                     /translation="MVDPASDEFRLVVPPDPRHVSTARIFAAAVARVAGADETTVEDI
                     KVGISEACSMSLRPPAADGPVEVSIVAEPERLRFEVIGDIPPERAQGEASEAQTPTPT
                     GIAATLGREVVSALFEDAEITGAGGRGTIRFSTPLGTA"
     gene            2510..2767
                     /locus_tag="VG709_08330"
     CDS             2510..2767
                     /locus_tag="VG709_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33119.1"
                     /translation="MRNELARGARDDKAGDSFAAAGPQPEAAEVESARLLAGKARDEL
                     LSRGLSDERIRRLADRYVAEDRGEDLDGFINWASRQPPDED"
     gene            2721..3134
                     /locus_tag="VG709_08335"
     CDS             2721..3134
                     /locus_tag="VG709_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWC33120.1"
                     /translation="MGSSTGLRASPPTKIEVDLPPGRDAPAQARRVLDRLDGLDDATR
                     EDARLVVSELVGNSVRHADLRPDDHIRLEVEDGSDAVRIRVVDPGPGFEPEVLAGPSL
                     RASQWGLFLVDRIASEWGVASEGTTAVWCEIDRRS"
     gene            complement(3139..>3486)
                     /locus_tag="VG709_08340"
     CDS             complement(3139..>3486)
                     /locus_tag="VG709_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWC33121.1"
                     /translation="GLTSVVVGVLFLLSLFLSPLAGVIPPEATAPVLVIVGYFMMTLV
                     RLIDWDDPSIGIPALLTMVVMPFTFSITNGVAAGFIAYSVIALLRGRWRDVHWLLFLV
                     SAVFVWYFVRGLI"
BASE COUNT          559 a         1212 c         1216 g          499 t
ORIGIN      
        1 ccaggcggcg gcgcgactgg cggggcggca cccgaaggtc cgggcggtcg acatcgtgga
       61 ggtcgatccc gaacgcgacg ttgccgacgt gaccgtgatg gccgcggcat cgtgcctact
      121 cgcattcgcc gcgggcctgg ccgggcgacc gacgtcctag ggacgcacgc cgaatggttc
      181 gcggcgcctc tatgaagggt tccggacgag ccgctcggag gggctcgagc gctactcgaa
      241 gatcccgagc acgtacctgg cgaggaacca gaggatcgcc acgacgatca ggaagatgaa
      301 cccgtacttg acggcggcgt acccggtcca ccccgacgca ggatcgcgcg ccggtgccgg
      361 ctctggtgcg tggctgtgag acatgtcgtg atgggtcacg gtggcctccg atctcgggcg
      421 ctcgcttccc ttgttccccg cgccgaggcg ctcgaaacgg actagacctc ctcgccgacg
      481 gcgtcgcgca gctgccggag cgtccgcgag agcagccggg agacgtgcat ctgggagatg
      541 ccgagctcgt ccgcgatctg cgtctgcgtg aggccgcgga agaaccggag atagaggatc
      601 ctgcgctcgc gatacggaag ctcacggagg accggcgcga cgctggtcca gctctcgagg
      661 agctcgtagg tgtcatcctc ggctccgagt ttctggatcg aggtggcgcc ctcctcgtcg
      721 gtgggcgcgt cgagcgacgc cgtcgagtac gcgtgcgccg tctccatcgc ctcgaggacc
      781 tcctcgtcgg agagatcggt acgcttcccg atctctcgga cggtcggcgc gcggcctagg
      841 tcctgataga ggtccgtcac ggcgcggccg accttgaggc ccgactcctg caggcgccgg
      901 ggcacgcgca gcgcccaccc cttgtcgcgg aagtgccgct tgagctcgcc gacgatcgtg
      961 gccgtggcgt acgtcgagaa cttcacgcct cgatcgacgt cgaagcggtc gatggccttg
     1021 atgagaccga tgctcgcgac ctggatcaag tcgtcgaggg gctcgccgcg accgtagaag
     1081 cgccgcgcga ggtactccgc gagcggcttg tacatctcga cgagctgctc ccgagccgca
     1141 tcgtcgggca gcgcccggaa cagctcgtcc gagcgcttgt cctggagctc cgcatcggtc
     1201 tgcgcctcgg cggccacgtg gttcgtcgcg tccgcggtca cgccggcgct ccgatcctct
     1261 tgacgaggtg gagcgcggga ccgtcgccgc tccgctccca gcgcgcctcg tcggtcagcg
     1321 cgcgcagcac ctgccacgcc atcgaacgct cggcctcgtc gggcggccac ttggcggccc
     1381 gcacgtcggc ggagacggtc gcctcgatct cgccccgtcc gtcggcgaca cgcatactca
     1441 gcgagcccgg gcgcagggga agcagctgcg cgcaggcctc gtcgaccgcg atcttgaggt
     1501 cctcgatcgc gtcgtacggg aagtcgagtc tggccgccat ccctgccacg accgaacgca
     1561 gaacctgcac gtactcgggt cgggcgggaa cggtgaccgt caggtctccg gacacgttgg
     1621 tcgcctatac tagcgacctc ttccatccac tcgactgcgg gggaacatgg ctgagatcaa
     1681 cttggtggtt gaggcgaacg agcgagggcc gtggactgtc ctggctctcg agggcgagat
     1741 cgacctctac accgcgccca agctgaagga gcagatctcc gagctggtcg cgaccgaccg
     1801 tgcccagctc gtcgtcgacc tctcgcgcgt cgagttcctg gactcgaccg gcctcggcgt
     1861 gctgatagga gggctgaagc gtgcccgaga gaagggcggc gagctcgccc tagccggcgc
     1921 caccgagccg gtgcggaagg tgctgtcgat caccggcctc gacaaggtct tcccgctcca
     1981 cgacgacgtg gacgtggcga ccggcgcgta gcgccatggt cgaccccgcg tccgacgagt
     2041 tccggctcgt ggtcccgccg gatccgcgcc acgtctcgac agcgaggatc ttcgcggccg
     2101 cggtcgcgcg cgtcgccgga gccgacgaga ccaccgtcga agacatcaag gtcggcatct
     2161 ccgaggcatg ctcgatgtcg cttcggcccc cggcggcaga cggccccgtc gaggtctcga
     2221 tcgtcgccga gcccgagcgg ctgcggttcg aggtcatcgg cgacatcccc cccgagcgcg
     2281 ctcagggcga agcctcggag gcccagacgc ccacgccgac cggcatcgcc gcgacgctgg
     2341 ggcgcgaggt cgtctcggcg ctcttcgagg acgccgagat cacgggggcc ggggggcgcg
     2401 gaacgatccg cttctcgacg ccgctcggga cggcctagtc gcttccgaaa accggcacag
     2461 atcccgaaga agacgaatac cgcgtccgtc cagcgggaaa gagcacggcg tgagaaacga
     2521 gctcgcccgc ggggcacgcg acgacaaggc cggtgactcg tttgccgcgg ccggtcccca
     2581 gcccgaggcc gccgaggtcg agagcgcccg actcttggcg gggaaggccc gcgacgagct
     2641 cctctcacgc ggactgtcgg acgagcggat ccgcaggctc gcggaccgat acgtggcgga
     2701 ggatcgcgga gaggacctcg atgggttcat caactgggct tcgcgccagc cccccgacga
     2761 agattgaggt cgacctgccg ccggggcgag acgcccccgc gcaggcgagg cgggtactag
     2821 accggctcga cggcctcgac gacgcgacgc gcgaggacgc gcgcctcgtc gtgagcgagc
     2881 tcgtcggcaa cagcgtccgc cacgcggacc tgcgcccgga cgaccacatc cggctcgaag
     2941 tcgaggacgg ctccgacgcc gtccgcatcc gcgtcgtcga cccggggccc gggttcgagc
     3001 ccgaggtcct cgccggcccg tcgctccgcg cctctcagtg gggcctgttc ctcgtcgacc
     3061 ggatcgcctc cgagtggggc gtggcgtcag agggcacgac ggcggtctgg tgcgagatcg
     3121 accgccggtc gtgaccggct agatcaagcc tcgcacgaag taccagacga agacggccga
     3181 caccaagaac agcagccagt ggacgtctcg ccagcgtcct cgtaggagcg cgatcacgct
     3241 gtacgcgatg aatccggccg cgacgccgtt cgtgatgctg aacgtgaacg gcatcaccac
     3301 catcgtgagc aacgccggga tcccgatcga cgggtcgtcc cagtcgatca ggcgaaccag
     3361 cgtcatcatg aagtagccca cgatcacgag caccggcgcg gtggcctcgg gcgggatgac
     3421 gccggcaagc ggtgatagga acagcgacag caggaacagc acccccacca cgacggacgt
     3481 cagccc
//
LOCUS       DASZOB010000530         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_191541, whole genome shotgun sequence.
ACCESSION   DASZOB010000530 DASZOB010000000
VERSION     DASZOB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191541"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2205)
                     /gene="cphA"
                     /locus_tag="VG709_08345"
     CDS             complement(<1..>2205)
                     /gene="cphA"
                     /locus_tag="VG709_08345"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWC33122.1"
                     /translation="LSPDLRILQTQVFRGPNYYSYEPAIRLLVDLGSLEHWPSNTLPG
                     FVEALLEHLPGLREHACSVGRPGGFVERLEDGTWLGHVAEHVAIDLQRQTGAHVYRGK
                     TRTAGEPGRYNVIYGYSEEQVGVEAGKFAVRLVNHLVEPEPDLDFLAELEGLIRLAER
                     RAFGPSTQALVDEAASRDIPYIRLNEQSLVQLGQGKFQRRIRATMTSMTPALAVDIAG
                     DKSLTNRLLEAAGLPVPRSAIVTTEDEAVAAARRIGFPVVTKPLDGNHGRGVSLELTT
                     EADVRAGFARALAEARRPYAVVESFVTGNDYRVLVIDGQMVAVAQRVPAHVVGDGKHT
                     VRQLVDITNEDPRRGIGHEKVLTRIKIDAAAGELVHKQGYGLDDVPPDGEMVKLAQTG
                     NMSTGGISIDRTWEAHEENVDVAEEAARVVGLDVAGIDFLTPDISQPVRETGGAIVEV
                     NAAPGFRMHTHPTEGEAQYVAKPVIDLLFPPGTPSRIPIVAVTGSNGKTTTVRMIAHI
                     FARLGRKVGMTTTDGIYIDERLVRKVDASGPKSATMVLQNPRIDFAVFEVARGGILRE
                     GLGYGRNDVAVVLNVTGDHLGIREIDSLEDLAAVKRVIVEAVPRTGHAVLNADDPLVA
                     AMRRHCSGSVTLFTMQERNELVERWVRRGGKAVVIERDSRGERIVIRDGRRSMPVAYV
                     HLLPITFEGRARMNVQNALAAAGAAHAAGAHLHDIRQALRSFAPSIFQTPGRL"
BASE COUNT          305 a          777 c          766 g          357 t
ORIGIN      
        1 caggcgtccg ggcgtctgga agatcgacgg cgcgaagctg cgcagcgctt gccgtatgtc
       61 gtgcaggtgc gcgccggcgg cgtgcgcggc gccggccgcc gcgagcgcgt tctggacatt
      121 catccgcgcc cgtccctcga acgtgatcgg gaggagatgc acgtaagcga ccggcatcga
      181 tcgccgcccg tcgcggatca cgatgcgctc gccgcgcgag tcgcgctcga tgacgacggc
      241 cttcccaccg cgacgcaccc atctctcgac gagctcgttc cgctcctgca tcgtgaacag
      301 cgtcacggac ccgctgcagt gccgacgcat cgccgctacg agcgggtcgt cggcgttgag
      361 gacggcgtgg cccgtgcgcg ggacggcctc gacgatcacg cgcttcacgg ccgcgagatc
      421 ctcgagcgag tcgatctcgc ggatgccgag gtggtcgccg gtcacgttca gcacgaccgc
      481 gacgtcgttg cgcccgtacc cgagcccctc gcgcaggatg ccgccgcgcg cgacctcgaa
      541 caccgcgaag tcgatgcgcg ggttctggag gaccatcgtg gccgacttcg gcccgctcgc
      601 atcgaccttc cgcacgagcc gctcgtcgat gtagatccca tccgtcgtcg tcatgccgac
      661 cttgcgtccc aagcgcgcga agatgtgcgc gatcatccgg acggtcgtgg tcttcccgtt
      721 cgacccggtc accgcgacga tcgggatccg cgacggcgtc cccggcggga acagcaggtc
      781 gatcaccggc ttcgcgacgt actgcgcctc tccctccgtg ggatgcgtgt gcatgcggaa
      841 gcccggcgcg gcgttcacct cgacgatggc gccgcccgtc tcacggaccg gttgggagat
      901 gtcgggcgtc aggaagtcga tccccgcgac gtcgagcccg accacgcgag ccgcctcctc
      961 ggcgacgtcc acgttctcct cgtgcgcctc ccacgtgcgg tcgatcgaga tcccgccggt
     1021 cgacatgttc cctgtctgcg cgagcttgac catctctccg tccggcggga cgtcgtcgag
     1081 cccgtacccc tgtttgtgga cgagctcccc agcggcggcg tcgatcttta tccgcgtgag
     1141 gaccttctcg tgtccgatac cccgtcgcgg gtcctcgttc gtgatgtcga cgagctgccg
     1201 gaccgtgtgc ttcccgtcgc cgacgacgtg cgcgggaacg cgctgggcga ccgcgaccat
     1261 ctgtccgtcg atgacgagca cccggtagtc gttgccggtg acgaagctct cgacgacggc
     1321 gtaggggcga cgcgcctctg cgagggcacg agcgaagccg gcgcgtacgt cggcctcggt
     1381 cgtcagctcg agcgagacgc cgcgtccgtg gttgccgtcg agcggcttgg tcacgaccgg
     1441 gaaaccgatg cgacgtgccg cggcgacggc ctcgtcctcg gtcgtgacga tcgccgaccg
     1501 cgggaccgga agcccggccg cctcgagcag ccggttcgtg aggctcttgt cgccggcgat
     1561 gtcgacggcc agcgccggcg tcatcgaggt catcgtcgcg cggatccggc gctggaactt
     1621 cccctggccg agctgcacga gcgactgctc gttgagccgg atgtacggga tgtcgcgact
     1681 cgcggcctcg tcgacgagcg cctgcgtcga ggggccgaag gcccgtcgct cggcgaggcg
     1741 gatgagcccc tcgagctccg cgaggaagtc gaggtcgggc tccggctcga cgagatgatt
     1801 gacaaggcgt accgcgaact tcccggcctc gacgccgacc tgctcctcgc tgtagccgta
     1861 gatgacgttg tagcgccccg gctctcccgc ggtgcgcgtc tttccgcggt agacgtgcgc
     1921 gccggtctgg cgctggaggt cgatcgcgac gtgctcagcg acgtggccga gccacgtccc
     1981 gtcctcgagc cgctcgacga acccgccggg acgcccgacc gagcatgcgt gctccctcaa
     2041 cccggggagg tgctcgagca gcgcctcgac gaagcccggg agcgtgttcg agggccagtg
     2101 ctcgagcgag ccgagatcga cgaggagcct gatcgccggc tcgtacgagt agtagttcgg
     2161 gccccggaac acctgggtct ggaggatccg gaggtcgggg ctgag
//
LOCUS       DASZOB010000531         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_180772, whole genome shotgun sequence.
ACCESSION   DASZOB010000531 DASZOB010000000
VERSION     DASZOB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180772"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VG709_08350"
     CDS             complement(<1..377)
                     /locus_tag="VG709_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33123.1"
                     /translation="MREAWLATPGRVPYGAAYEAMHELVDRRVAAEVPDALVLLEHPP
                     VYTLGRRADPSHVLFDEERLSSMGAEVHPVDRGGSVTFHGPGQLVGYPVLDLGPAADV
                     IGYLRMLEETVIRTCTDRGAEVGR"
     gene            complement(374..1834)
                     /locus_tag="VG709_08355"
     CDS             complement(374..1834)
                     /locus_tag="VG709_08355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWC33124.1"
                     /translation="MATPVTMPQLGETVVEGTIVKWLKGEGDSIERDEPLFEISTDKV
                     DTEVPSPIAGTLKEIKVPEGETVSVGTELAVIDEGGAAGDGQAAGDGQAAAEEPAQAQ
                     QEQPQRQQEQPRAEEPQQAETPAGAEQPSATPEPERQPDSQAPPVTGERGAPAAAKVE
                     APPDRGPRSQILSPLVRRLAEEHQVDLSRVQGSGAGGRITKKDVLAFTEQRGAEPERA
                     PLRAVAEEEAAPAAEAAPEPAAPAARAPAAGAREEVVRISHIRKRIAENMQSVLQNVA
                     RAWNLVEIDMENIVRLRARAKDAFKEREGISLTYMPFVARAVCDALLAYPDVNASLDL
                     EAGTATYRRYVNLGIAVSYDAGLIVPVVRGADEMNIIGLARAINDVATRARSKKLRPE
                     EVHGGTFTITNPGPFGSLISVPIPNPGETAILAFDAVEKRPVVRDDAIAIRNMVYLSM
                     SWDHRMIDGAVAAQFLARLKQNLETWDFTPELQGYL"
     gene            complement(1854..2810)
                     /locus_tag="VG709_08360"
     CDS             complement(1854..2810)
                     /locus_tag="VG709_08360"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWC33125.1"
                     /translation="MAIAEARWAEMERDDRVFMLGEDIGVYGGAFKVTEGFIDRFGAD
                     RVLDTPICEETIVGMSVGAAMEGFRPVAEFQYADFMTSGFDELVTVAARYHYRSGVPI
                     PAVFRGPSGGGVRASAFHSTNPEPWFAHAPGLKVICPAFPTDAKGLLKAAIRDDNPCV
                     YLEHKWIYRRVREQVPEDPDFTIPIGKADVKREGDDISIITYGGMVHRALEAAEELAD
                     EGVSAEVLDLRTIYPLDREAILQTVEKTSRALVLYESLRFLGVGAEVSAVIAEDGFEL
                     LDAPVMRLAPPHTPVPFSPPLEDAFLPQVADVVEAVQKLSKW"
BASE COUNT          367 a         1039 c         1002 g          458 t
ORIGIN      
        1 cgaccgacct cggcgccgcg gtcggtgcac gtgcggatca cggtctcctc gagcatccgc
       61 aggtatccga tgacgtccgc ggcggggccg aggtcgagga ccgggtaccc gacgagctga
      121 ccggggccgt ggaacgtgac gctgccgccg cgatccaccg ggtgcacctc ggcgcccatc
      181 gacgagagcc gctcctcgtc gaacagcaca tgcgacggat cggcgcgccg gccgagcgtg
      241 tacaccggcg ggtgctcgag cagcacgagg gcgtcgggca cctccgccgc gacgcggcga
      301 tcgacgagct cgtgcatcgc ctcgtaggcg gcgccgtagg ggacacgccc cggcgtcgcg
      361 agccacgcct ctctcacagg tagccctgca gctcgggcgt gaagtcccac gtctcgaggt
      421 tctgcttgag ccgcgcgagg aactgcgccg cgacggcgcc gtcgatcatg cggtggtccc
      481 acgacatcga cagatagacc atgttccgga tcgcgatcgc gtcgtcgcgg acgaccggcc
      541 gcttctcgac cgcgtcgaac gcgaggatcg cggtctcgcc ggggttgggg atcgggacgc
      601 tgatcagcga gccgaacggc cccgggttcg tgatcgtgaa cgtgccgccg tgcacctcct
      661 cggggcgcag cttcttcgac cgcgcccgcg tcgcgacgtc gttgatcgcg cgcgcgaggc
      721 cgatgatgtt catctcgtcg gctccacgga ccaccggcac gatcaggccg gcgtcgtacg
      781 acaccgcgat gccgaggttc acgtagcggc ggtacgtcgc ggtcccggcc tcgaggtcga
      841 ggctcgcgtt gacgtcgggg taggcgagca gcgcgtcgca caccgcgcgg gcgacgaacg
      901 gcatataggt gagcgagatc ccctcgcgct ccttgaacgc gtccttcgcg cgcgctcgga
      961 gccgcacgat gttctccatg tcgatctcga cgaggttcca cgcgcgggcg acgttctgga
     1021 ggaccgactg catgttctcg gcgatccgct tgcggatgtg cgagatgcgt acgacctctt
     1081 cgcgggctcc ggcggcaggt gcgcgggccg cgggggcagc gggctccgga gccgcctcgg
     1141 ccgccggcgc cgcctcctcc tccgcgacgg cacggagcgg cgcgcgctcc ggctcggccc
     1201 cgcgctgctc ggtgaacgcg aggacgtcct tcttcgtgat ccgtcctcct gcgccggatc
     1261 cctggacccg cgagaggtcg acctggtgct cctcggcaag cctccgtacg agcggcgaca
     1321 ggatctggct ccgcgggcct cggtcgggcg gcgcctccac cttcgcggcg gccggcgcgc
     1381 cccgctcgcc ggtcaccggc ggagcttggc tgtccggctg gcgctcgggc tccggagtag
     1441 ccgacggctg ctccgctccg gcgggcgtct ccgcctgctg cggctcttcg gcgcgcggct
     1501 gttcctgctg ccgctgcggc tgctcctgct gcgcctgcgc gggctcctcg gccgcggcct
     1561 gaccgtctcc ggcggcctgt ccgtcgccgg cggcgccgcc ctcgtcgatc accgcgagct
     1621 cggtgccgac gctcacggtc tcgccctccg ggaccttgat ctccttcagt gtgcccgcga
     1681 tcggcgatgg cacctcggtg tcgaccttgt cggtcgagat ctcgaacagc ggctcgtctc
     1741 gctcgatgct gtcgccctcg cccttgagcc acttgacgat ggtgccctcc accaccgtct
     1801 cgcccagctg cggcatcgtc acgggcgtcg ccaatgtcgg cctccttctg ggttcaccac
     1861 ttggacagct tctggaccgc ctccaccacg tccgccacct gggggaggaa cgcgtcctcg
     1921 agcggcggcg agaacgggac cggcgtgtgc gggggtgcga ggcgcatcac cggggcgtcg
     1981 aggagctcga acccgtcctc ggcgatcacg gcggagacct cggcgccgac gccgaggaac
     2041 cgcaggctct cgtacaagac gagcgcgcgg ctcgtcttct cgacggtctg gaggatcgcc
     2101 tctcggtcga gcggatagat cgtccggagg tcgagtacct ccgccgagac gccctcgtcg
     2161 gcgagctcct cggccgcctc gagcgcgcgg tgcaccatcc cgccgtacgt gatgatcgag
     2221 atgtcgtcgc cctcgcgctt cacgtcggcc ttgccgatcg ggatcgtgaa gtccggatcc
     2281 tcggggacct gttcgcgcac gcggcggtag atccacttgt gctcgaggta cacgcacggg
     2341 ttgtcgtcgc ggatcgcggc cttcagcaga cccttcgcgt cggtcgggaa cgccggacag
     2401 atcaccttga ggccgggcgc gtgcgcgaac cacggttcgg ggttcgtcga gtggaaggcg
     2461 ctcgcgcgca cgccgccccc agacgggccg cggaacaccg ccgggatcgg gacgcccgaa
     2521 cggtagtggt accgggcggc gaccgtgacg agctcgtcga agccgctcgt catgaagtcg
     2581 gcgtactgga actccgcgac gggacggaac ccctccatcg cggccccgac cgacatgccg
     2641 acgatcgtct cctcgcagat cggtgtgtcg aggactcggt cggccccgaa gcggtcgatg
     2701 aacccctcgg tgaccttgaa cgcgccgccg tacacgccga tgtcctcgcc gagcatgaag
     2761 acgcgatcgt cgcgctccat ctccgcccac cgcgcctcgg cgatcgcgat gaggtaggtg
     2821 gcctcgccgt tctccgtgcg cttgccgcgc ggctcggcgc cgtggg
//
LOCUS       DASZOB010000532         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_49319, whole genome shotgun sequence.
ACCESSION   DASZOB010000532 DASZOB010000000
VERSION     DASZOB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49319"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VG709_08365"
     CDS             complement(<1..528)
                     /locus_tag="VG709_08365"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HWC33126.1"
                     /translation="MQRPVRVPVLPFLLVFAILFVVGSARQPEPKEPFNYSRFLAHVE
                     SGGVERVRLDAGDNSIEVRPKRGDGYKTAYPDNTEQALVDRLRAANIPIAVKAKRGSG
                     IWGSLIYVVPFVLFLLFWLFIIRRMSGAGGQMTRFAKSKAKLQTALDRPDVTFRDVAG
                     VEEAVEELHEIKEFLE"
     gene            complement(719..>1669)
                     /locus_tag="VG709_08370"
     CDS             complement(719..>1669)
                     /locus_tag="VG709_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWC33127.1"
                     /translation="ALLRPPAQWLHARGASRAAAALLVMGGALAVLAALATVIVPPFV
                     DQLDQLGRDLRAGVEQAGDWLLEGPLNLSEQELDGYLDSAERSLRESGGTIATGVLGG
                     ALLVLELIAGVGLAVVITFFFLKDGDRIAAWLVGLFPKQRRGEVEEVGRRAWETLADY
                     LRGMTVVALFDSVFIGLVLVIIGVPAALPLAVFTFFGAYIPIAGALITGIAAVLVALV
                     AEGVTTAAIVALAVLVVQQVESNLLQPFVVGRAVAVHPLAILLAVTAGAVLGGVAGAI
                     VAVPVTAVGARVGGYLRERTAEASLPLDAVDASAPARADG"
BASE COUNT          285 a          579 c          583 g          222 t
ORIGIN      
        1 ctcgaggaac tccttgatct cgtggagctc ctcgacggcc tcttcgacgc cggcgacgtc
       61 gcggaaggtg acgtctggcc gatcgagcgc agtctgcagc tttgccttcg acttcgcgaa
      121 gcgcgtcatc tgacccccgg cccccgacat ccggcgaatg atgaacagcc agaagaggag
      181 aaagaggacg aacggcacga cgtagatcaa ggacccccag attccactgc cacgcttggc
      241 cttgacggcg atggggatgt tcgcggcgcg gagcctgtcg acgagtgcct gctcggtgtt
      301 gtcggggtat gccgtcttgt agccgtcgcc gcgcttgggc ctcacctcga tcgagttgtc
      361 gccggcgtcg agccggaccc tctcaactcc tccggactcg acgtgggcca ggaaccgcga
      421 gtaattgaaa ggctccttcg gctcaggctg acgggcgcta ccgactacga acaggatcgc
      481 gaacacgagg aggaacggca gaacaggaac tcgcactggc ctctgcatcg gagcgcgcgg
      541 tcagtcctcg acgccgcgac cccgtgccgt gtgccgcggt ccccccgcac ggggtctgag
      601 acgggtcatg gcgcgctcga attgaactcg agcgacaggc gaggggccat ggcccccacc
      661 cggcgaaacg ggtggcgatc cgtgtggcac cgcagcgagg aacgtagcgc gcctccagtc
      721 atccgtccgc ccgggctggc gcggaagcat cgactgcgtc gagcgggagg ctcgcctcgg
      781 cggtccgctc gcgcaggtat ccgccgacgc gcgcgcccac cgccgtgacc ggaaccgcga
      841 cgatcgcgcc ggcgacgccg ccgaggaccg ctccggccgt cacggcgagc aggatcgcga
      901 gcgggtgcac cgcgacggct cgcccgacga cgaagggctg gagcagattg ctctcgacct
      961 gctgtaccac gaggactgcg agcgccacga tcgcggccgt ggtcacgccc tccgccacga
     1021 gcgcgacgag gacggcggcg atcccggtga tcagcgctcc ggcgatcggg atgtaggcgc
     1081 cgaagaacgt gaagaccgcg agcgggagcg cggcgggtac gccgatgatg acgagcacga
     1141 gcccgatgaa caccgagtcg aagagcgcca cgaccgtcat gccgcgcagg tagtccgcca
     1201 gtgtctccca ggcgcgccgc ccaacctcct ccacctcgcc gcggcgctgc ttcggaaaga
     1261 ggccgacgag ccaggccgcg atccggtcgc cgtctttcag gaagaagaag gtgatgacga
     1321 cggcgaggcc gacgccggct atcagctcaa gcacgagcag tgcgccgccg aggacgcccg
     1381 ttgcgatcgt cccgccgctc tcacgcagcg agcgctcggc gctgtcgagg tatccgtcga
     1441 gctcttgctc ggagaggttg agcggtccct cgagcagcca gtctccggcc tgctcgacgc
     1501 ccgcgcgaag gtcgcgtccg agctggtcga gctggtcgac gaacggcggg acgatcaccg
     1561 tcgccagggc cgccagcacg gcgagcgcac cgcccatcac gaggagcgcg gcggccgccc
     1621 gcgaggcccc gcgcgcgtgg agccactggg cgggcggtct cagcagagc
//
LOCUS       DASZOB010000533         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_49462, whole genome shotgun sequence.
ACCESSION   DASZOB010000533 DASZOB010000000
VERSION     DASZOB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49462"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..738)
                     /locus_tag="VG709_08375"
     CDS             complement(112..738)
                     /locus_tag="VG709_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWC33128.1"
                     /translation="MSVDPHALRARRLTGPTATAAPRASSNGWWGMMLLIATEATLFA
                     VVIASYYYLSWRSDLGWPQGDIAEPKLERPIAMTVVLFASSMPMFVAETGIRRGKQTR
                     LRIGLGATFLLGVAFLALQGWEYSVKVKEFTPETNAYASLFYVITGLHGSHVLAGLLV
                     LAWVQVRAWRGAYGVERHLGVQMGAVYWHFVDAVWAVIFPTIYVYPHL"
     gene            complement(735..>1683)
                     /locus_tag="VG709_08380"
     CDS             complement(735..>1683)
                     /locus_tag="VG709_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWC33129.1"
                     /translation="ISMTLFSAASFTIAIPSAIQVFAWIATALAGRPLLRTPMLFALG
                     FVLVFVIGGVTGVMFASVPFDQQITDSYFVVAHFHYVLFGGAVFPILAGVYFWLPKMS
                     GRMYAERLGQASFWLVFAGFNLTFFPMHVSGLLGMPRRIYTYPTGMGWDVYNLLATVG
                     AFVLAVGLAAVVANVAWTVRRGAPAGADPWRADTLEWSLPSPPPPYAFPVIPTVTTAH
                     PNWDVEDRVEDARRLARGDLVLDDGHQTLATTVLEGEPDDVLDMPSEELSPLLLALAL
                     AVVFTAFLFELWIMSAIGGVLALGALALWHRPKRELQEQ"
BASE COUNT          307 a          553 c          619 g          204 t
ORIGIN      
        1 agccgacgag gagatgggcg gtccaggcgg ccggcggccc gaggaaggcg agccagacga
       61 gtccgcggtc gcgccgcgtg agcttgcgcg gctcgtgcgg ggccgccgcg ctcatagatg
      121 cggatacacg tagatggtcg ggaagatcac cgcccagacg gcgtcgacga aatgccagta
      181 gacggccccc atctgcacgc cgagatggcg ctcgacgccg tacgcgccgc gccaggcgcg
      241 gacctgcacc cacgcgagca cgagcagccc cgcgaggacg tgcgaaccgt gcagcccggt
      301 gatgacgtag aagagcgacg cataggcgtt cgtctccggc gtgaactcct tcaccttcac
      361 gctgtactcc cacccttgaa gcgcgaggaa cgcgacgccg aggaggaagg tcgcgccgag
      421 gccgatgcgg aggcgagtct gcttgccgcg ccggatcccc gtctcggcga cgaacatcgg
      481 catcgagctg gcgaagagga cgaccgtcat cgcgatgggc cgctcgagct tcggctccgc
      541 gatgtcgccc tgcggccagc cgaggtcgga tctccagctc aggtagtagt aggacgcgat
      601 cacgaccgcg aagagggtcg cctcggtcgc gatcagcagc atcatgcccc accagccgtt
      661 cgaggaggcg cgcggcgccg ccgtcgcggt cggccccgtc agccgccgcg cgcggagggc
      721 gtgagggtcg acgctcactg ctcttggagc tcccgtttcg gccggtgcca cagcgcgagc
      781 gccccgagcg ccagcacgcc gccgatggcc gacatgatcc agagctcgaa caggaacgcc
      841 gtgaagacga cggcgagcgc gagcgcgagg aggagcgggg acagctcctc gctcggcatg
      901 tcgagaacgt cgtccggctc cccctcgagc accgtcgtcg cgagcgtctg gtggccgtcg
      961 tcgaggacga gatcgccgcg ggcgagccgg cgcgcgtcct cgactcggtc ttcgacgtcc
     1021 cagttgggat gcgcggtcgt gacggtcggg atgacgggaa aggcgtacgg cgggggcggc
     1081 gacggaagcg accattcgag cgtgtcggct cgccacgggt cggcgccggc aggcgcgccg
     1141 cggcgaacgg tccaggcgac gttcgcgacc acggcggcaa ggccgaccgc gagcacgaac
     1201 gcgccgaccg tcgcgaggag gttgtacacg tcccatccca tgccggtcgg gtatgtgtag
     1261 atgcgccgcg gcatccccag gagcccggag acgtgcatgg ggaagaaggt gaggttgaag
     1321 ccggcgaaca cgagccagaa gctcgcctgc ccaagccgct ccgcgtacat ccgcccgctc
     1381 atcttcggaa gccagaagta gacgccggcg aggatgggga agaccgcgcc gccgaagagc
     1441 acgtagtgga agtgcgcgac cacgaagtac gagtcggtga tctgctggtc gaacgggacc
     1501 gacgcgaaca tgacgcccgt cacaccgccg atcacgaaca cgaggacgaa gccgagggcg
     1561 aagagcatcg gcgtccggag gagcggacgc ccggcgagcg cggtcgcgat ccatgcgaag
     1621 acctggatcg cgctcgggat cgcgatcgtg aagctggcgg ccgagaagag cgtcatcgag
     1681 atc
//
LOCUS       DASZOB010000534         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_39011, whole genome shotgun sequence.
ACCESSION   DASZOB010000534 DASZOB010000000
VERSION     DASZOB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39011"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VG709_08385"
     CDS             complement(<1..501)
                     /locus_tag="VG709_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC33130.1"
                     /translation="MERDFGLSSEQREIVALVREFVGERVRPSIDAFEQRKEFPRELF
                     RTLGQMGLAGIPYSEKYGGGGQGYVTYLSVLEELATGSLSLTVGLSVHHLSAFGIHEF
                     GSDELKERFLPAMFAGEWLGAYALSEASSGSDAASLRARAARTDAAWRLDGAKRFISH
                     AGEADVY"
     gene            545..1957
                     /gene="cysS"
                     /locus_tag="VG709_08390"
     CDS             545..1957
                     /gene="cysS"
                     /locus_tag="VG709_08390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWC33131.1"
                     /translation="MRLYNTLTRSVEEVRPLEDRLVRMYTCGPTVYRPVHIGNLRTFL
                     LSDLVRRSLELEGYEVRQVVNITDVGHMVDDSSEEAIDRMELATSDEGTDPWTIAAKY
                     TDLFLRDTRRIGIEPAHVYPRATDHIGEMIEMSESLIRRGHAYALEDGTVYFDVRSFP
                     EYGRLSRNTLDQLQPGHRELERDDRKRHHADFALWKRAEPDRIMKWDSPWGEGFPGWH
                     IECSAMSIAHLGHRFDIHTGGVDLVFPHHEDEIAQSDGATGHTVVGLWAHGGHLLAEG
                     QKMSKSTGNVWSLADIEERGHDPLAFRLLCFQTRYRSQMDFRWDALAAADRTLRRWRQ
                     RMADWSPAPRDGLSPEAIELDRRFREAVADDLDMPTAVKIGGETVSSRVADGEKYELL
                     SSWDRMLGLDLERLAREGFDVPPDVQRLVDERDDARRRKDFAASDEMRNRLIAMGWEV
                     LDTSEGTTVRPLGGRAPSGA"
     gene            1973..2398
                     /locus_tag="VG709_08395"
     CDS             1973..2398
                     /locus_tag="VG709_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33132.1"
                     /translation="MGAPPTQPNGSTVWRPRLPLGIVVASAVVALVAVSSLIYLLYNR
                     TRGPAEVLREFATAVDAGDCSTAYDLLSSDSRTEIDDERFCSEELGRLDEDLDADFTL
                     DRVVYHRRTQTADLTLAGDPSGPWRLKRHGNSWRVVLPA"
     gene            complement(2395..3615)
                     /locus_tag="VG709_08400"
     CDS             complement(2395..3615)
                     /locus_tag="VG709_08400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWC33133.1"
                     /translation="MRRFTSATQEGSNVPLIQIPDELHDLRNTLRDFIDREIRPTEES
                     YRQQIQDARYDALKDERRKIRKRAAELGFWTIHMPEQVGGMGLPYLGQVLLIEEATRH
                     GLFLTHNEAIFPIVTGPTPVYLDCTDAQREKYLDPLMRADKFACFALTEPDAGSDATR
                     IKTRAGKQDGEWVLNGRKHFITGGDEADFAVVFAVTDAEKRAKGGITAFLVDADSPGY
                     SVSRMQHTMSPIQNPAELTFEDVRVPEENVLGKVGFGFYSAVKGINGARLQIAATALG
                     MAQHLIERMIEYAGQRVAFERPIGSNQYVQGMIVDSFAELEQSRLLVYKLAADIDDGA
                     DGRREAALAKLVSTEMVGRVADRAIQVHGGNGYMTELGLEVWYRDVRAMRLYEGTSEI
                     LRTNVAKTLGLPSA"
     gene            complement(3642..>4845)
                     /locus_tag="VG709_08405"
     CDS             complement(3642..>4845)
                     /locus_tag="VG709_08405"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HWC33134.1"
                     /translation="YQGDLSDPIDIAVDAFVAKLSPDGGDLLYSTYLGGTEREIGTAV
                     DVRHGRAYVVGLTDSVDTGEPTSVDFPTTPNAPRMPPSPDETQAFATKFSTTGSEVVY
                     SLLLGGGSIDYATDLALDDEGNATIVGVTYSEDFPTRHPVLRREIQPEDAFVTRVDAA
                     GSALIYSSHIGGSLEDGASAVDVDGTGRAFVTGFTDSFDFPLREAVQWQHTGEDEAFL
                     VGLTASGRALRFSTYAGALSLSRTPHILQDANGIAARAGAIHLAGTTSWSEWNSGRSF
                     QRDAVTSGADAFAAVLGAVGGVFDHPREVTLRIRNHTSLRGRLTDPLGHLRCVRGQPV
                     DLRLRDRRRWSPLGRVVTDEEGRFRFPMGWDPVGDFRAVASSSIHPHAGRWHRCAATR
                     SKILLHRH"
BASE COUNT          779 a         1648 c         1639 g          779 t
ORIGIN      
        1 atacacgtcc gcctctccgg catgcgagat gaatctctta gcgccgtcga gccgccaggc
       61 agcatcggtg cgcgcggcgc gcgcgcgaag cgacgcggcg tcggacccgc tcgatgcctc
      121 ggacagcgcg tacgcgccga gccactctcc cgcgaacatc gccgggagga accgctcctt
      181 cagctcatcg gagccgaact cgtggatccc gaacgcggag aggtggtgca ccgagagccc
      241 gaccgtgagc gagagcgatc ccgtcgccag ctcctcgagc accgagaggt acgtgacgta
      301 cccctgaccc ccgcccccgt acttctcgga gtacgggatc ccggcgagcc ccatctggcc
      361 gagcgtgcgg aagagctcgc gcgggaactc cttacgctgc tcgaaggcat cgatcgaggg
      421 tcggacgcgc tcccccacga actcgcggac gagggcgacg atctcgcgct gttcgctcga
      481 caggccgaag tcgcgttcca cgagcaaccg tagagtaccg gacgcggcgg ctatcctctc
      541 ggtcgtgcgc ctctacaaca cgctgacgag gagcgtcgag gaggttcggc cgctcgagga
      601 ccgcctcgtt cggatgtaca cgtgcggtcc gacggtctac cggcccgtgc acatcgggaa
      661 cctccggacg ttcctgctct ccgacctcgt tcgccgcagc ctcgagttag agggctacga
      721 ggtcaggcag gtcgtcaaca tcaccgacgt cgggcatatg gtcgacgact cgtcggagga
      781 ggcgatcgat cgcatggagc tcgcgacgtc ggacgaggga accgatccat ggacgatcgc
      841 cgcgaagtac acggacctgt tcctccggga cactcgacgg atcggcatcg agccggcgca
      901 cgtctacccg cgcgcgacgg atcacatcgg agagatgatc gagatgagcg agtcgctgat
      961 ccggcggggg cacgcgtacg cgctcgagga cgggaccgtg tacttcgacg tccggtcgtt
     1021 ccccgagtac gggaggctct cccgtaacac gctcgatcag ctccaacccg ggcatcgcga
     1081 gctcgaacgc gacgaccgga agcgtcacca cgccgacttc gcgctgtgga agcgcgccga
     1141 gcccgaccgc atcatgaagt gggacagtcc ctggggggag gggttccccg ggtggcacat
     1201 cgagtgctcc gcgatgtcga tcgcgcacct cgggcaccgc ttcgacatcc atacgggcgg
     1261 cgtcgacctc gtgttcccac atcacgagga cgagatcgcg cagtccgacg gagccaccgg
     1321 gcacacggtc gtggggctct gggcgcacgg aggacacctg ctcgccgagg gccagaagat
     1381 gtcgaagtcg acgggcaacg tctggtcgtt ggccgacatc gaagagcgcg gtcacgaccc
     1441 gctcgcgttc cggctcctgt gcttccagac tcggtaccgc tcgcagatgg acttccgctg
     1501 ggacgccctc gccgcggccg accggaccct gcggcgctgg cgtcagcgga tggccgactg
     1561 gagccccgcg ccgcgcgacg ggctctcgcc cgaggcgata gagctcgacc ggcgcttccg
     1621 tgaggccgtc gccgacgacc tcgacatgcc gaccgcagtg aagatcggcg gcgagacagt
     1681 ctcgtcgcgc gtcgccgacg gcgagaagta cgagctgctc tcgtcatggg atcgcatgct
     1741 cgggctcgac ctcgagcgcc tcgcgcgcga ggggttcgac gtcccaccgg acgtccagcg
     1801 cctcgtcgac gagcgcgacg acgccaggcg ccggaaggac ttcgcggcgt cggacgagat
     1861 gcggaaccgc ttgatcgcga tgggatggga ggtgctcgat acgtcggagg ggaccacggt
     1921 ccgcccgctc ggcggtcggg ctccgagcgg cgcgtagtag gcttcggcgc cgatgggcgc
     1981 tccacccact cagccgaacg gctccaccgt gtggcgaccg aggctcccgc tcgggatcgt
     2041 cgtcgcctcg gcggtcgtgg cgctggtggc ggtctcgagc ctgatctacc tcctctacaa
     2101 ccgcacccgc ggaccggccg aggtgctccg tgagttcgcc accgcggtgg acgcgggcga
     2161 ctgcagcacc gcgtacgacc ttctgtcttc cgattcgcgg acggagatcg atgacgagcg
     2221 cttctgcagc gaggagctcg gtcggctcga cgaggacctc gacgccgact tcacgctcga
     2281 ccgcgtcgtg taccaccgcc ggacgcagac cgccgacctg acgctggccg gagacccgtc
     2341 cggtccgtgg cgcctcaagc ggcacgggaa cagctggcgg gtggtcctgc cggcctaggc
     2401 gctcggcagc ccgagggtct tcgctacgtt cgtgcgcagg atctccgacg tgccctcgta
     2461 cagccgcatc gcgcggacgt cccggtacca gacctcgagc ccgagctcgg tcatgtatcc
     2521 gttgccgccg tggacctgga tggcccggtc ggcgacacgt ccgaccatct cggtcgagac
     2581 gagcttggcg agcgcggcct cccggcgccc gtccgcgccg tcgtcgatgt cggcggcgag
     2641 cttgtagacg agcagccggg actgctcgag ctccgcgaac gagtcgacga tcatcccctg
     2701 gacgtactgg ttcgacccga tcggacgctc gaacgcgacg cgctgccccg cgtactcgat
     2761 catccgctcg atcaggtgtt gcgccatccc gagtgcggtg gcggcgatct gcagccgcgc
     2821 gccgttgatc cccttgacgg ccgagtagaa gccgaacccg accttcccga gcacgttctc
     2881 ctccggcacg cgcacgtcct cgaacgtgag ctccgcgggg ttctggatgg gcgacatcgt
     2941 gtgctgcatg cggctcaccg agtagcccgg cgagtcggcg tcgacgagga aggccgtgat
     3001 cccacccttc gcgcgcttct ccgcgtcggt gacggcgaag accaccgcga agtcggcctc
     3061 gtctccgccg gtgatgaagt gcttgcggcc gttcaggacc cactcgccgt cctgcttgcc
     3121 agcgcgcgtc ttgatccgcg tggcgtccga gccggcgtcg ggctcggtga gcgcgaagca
     3181 cgcgaacttg tcggcgcgca tcagcgggtc gaggtacttc tcgcgttgtg cgtcggtgca
     3241 gtcgaggtat acgggcgtcg ggcccgtgac gatcgggaag atggcctcgt tgtgcgtgag
     3301 gaacaggccg tggcgcgtcg cctcctcgat cagcaggacc tggccgaggt acgggagccc
     3361 catgccgccg acctgctccg gcatgtggat cgtccagaac ccgagctccg cggcgcgctt
     3421 gcggatcttg cgacgctcgt ccttgagggc gtcgtagcgc gcgtcctgga tctgctggcg
     3481 gtacgactcc tcggttggac ggatctcgcg atcgatgaag tcacgaagcg tattgcgaag
     3541 gtcgtgcagc tcgtcgggga tctggatcag cggcacgttg gagccctcct gcgttgcgga
     3601 cgtgaaccta cgcacgagta ggttatcacg gacgtctgaa ctcaatgccg gtggagaagg
     3661 atcttcgacc gtgtagcggc acagcggtgc catctgccgg cgtgcgggtg gatcgaggac
     3721 gacgcgacgg cccggaagtc gccgaccgga tcccatccca tcgggaatcg aaaccgccct
     3781 tcttcgtcgg tgacgacgcg gccgagcggg gaccaccttc gccggtctcg cagacgcagg
     3841 tcgaccggtt ggccgcgcac acagcggagg tgtcccaacg gatccgtcag ccgaccgcgc
     3901 aacgaggtgt ggttgcggat cctgagcgtg acctcgcgtg ggtgatcgaa cacgcccccg
     3961 accgcgccga gaacggcggc gaacgcgtcc gcgccagagg taaccgcgtc gcgctggaag
     4021 cttcggccag aattccactc cgaccacgac gtcgtcccgg cgagatggat cgctccagcc
     4081 cgggccgcga ttccgttcgc gtcctgaagg atatgtggcg ttcgcgagag gcttagcgcg
     4141 ccggcgtacg ttgagaaccg cagcgctcgt cccgaagccg tcaacccgac aaggaaagcc
     4201 tcgtcctcac ctgtgtgctg ccactgcacg gcttccctca gcgggaagtc gaacgagtcc
     4261 gtgaatccgg tcacgaacgc ccgtcccgtt ccgtcgacgt ccacggccga cgctccgtct
     4321 tcgagggacc cacctatgtg actcgagtag atgagcgcgg agccggcggc gtcgacgcgg
     4381 gtcacgaagg catcctccgg ttgaatctcc ctccgtagca cgggatggcg ggttgggaag
     4441 tcctcgctgt acgtgacgcc tacgattgtc gcgtttccct cgtcatcgag ggccagatcg
     4501 gttgcgtagt cgatgcttcc tcccccgagc agcagcgagt acacgacctc actgcccgtc
     4561 gtcgagaatt tcgtcgcgaa cgcctgagtc tcgtccggag atggcggcat gcgaggagcg
     4621 ttcggcgtcg tggggaagtc aaccgaggtg ggttcgccgg tatcgacgga gtcggtcaac
     4681 ccgacgacgt acgcccgccc gtgccgcacg tcgacagccg tgccgatctc ccgctcggta
     4741 cccccgaggt acgtcgaata gagcaggtcg cccccatccg gtgacagctt cgccacgaag
     4801 gcgtccacgg cgatatcgat cgggtcgctc aggtcgcctt gatag
//
LOCUS       DASZOB010000535         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_39145, whole genome shotgun sequence.
ACCESSION   DASZOB010000535 DASZOB010000000
VERSION     DASZOB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39145"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VG709_08410"
     CDS             <1..560
                     /locus_tag="VG709_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33135.1"
                     /translation="LILLVLGGFVALGAGLRSAQDAQQRRQEVQQFASQVDAAFARSG
                     IVQQIGPTPVPIPEIQSAIADLERRKLDPQRLRADAARWGEGASELSDSIAALGAESP
                     ALREARDLMRQSLRLYASIAQEIRVAATLDPGERSELVGPLQQQMQLAGSFFDFGWRS
                     LATERELAGIPVVEEPLAPPGFQAP"
     gene            603..1355
                     /locus_tag="VG709_08415"
     CDS             603..1355
                     /locus_tag="VG709_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWC33136.1"
                     /translation="MTVLLLVHAFPVDASMWEPQITGLGGDLQVLAPSLPGFGGESPP
                     PGAVLTMDAAADAMGEALDRAGVQSAVICGLSMGGYVAFSMWRRHRDRIDALVLADTR
                     AEPDDDAGRERRRTVAETARTRGSAAIAENPPALLSDGADDALWDRVKGWIRAQPGEA
                     IAAASLGMAERPDSRPILGEIDVPTTIVVGSADALTPPAMSESMAEAIPGAQLVVLDG
                     AGHLSNLEDPEGFNAAIEGILDRVGREGMQRP"
     gene            1399..>2862
                     /locus_tag="VG709_08420"
     CDS             1399..>2862
                     /locus_tag="VG709_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWC33137.1"
                     /translation="MDLTTFADRYPFPLDAFQRGAIDALAAGESVLVAAPTGSGKTVV
                     AEYAIEQALARGRKAFYTTPLKALSNQKFGDFVARYGADEVGLLTGDNSINGEAPVVV
                     MTTEVLRNMLYEQSDTLDDLEVVAMDEVHYLQDPYRGAVWEEVLIHLPLSVSVVCLSA
                     TVSNAEEFGEWLTTLRGTTRVVIEEKRPVPLEQLYMAGRELHPMYVERRGAPMPNPEV
                     AALTRRERRIGESRGRAPEGRQPSRAARSQGEGRRLYVPRREDVVEVLGDQGMLPAIY
                     FVFSRAGCNRSVEFLMESGLRLTDANEERRIRELVELRAAWIEDDDLAALGFATFREA
                     LAAGIAAHHAGMVPVFKETVEELFAAGLVKVVFATETLSLGINMPARSVVIESLWKFS
                     GERHELLTPGEYTQLTGRAGRRGIDPHGYAVVLHQRDVDFERVAGLATTRTYELRSSF
                     RPSYNMTVNLVRTYTPEETHHLLNSSFAQFQADRGVVG"
BASE COUNT          416 a          981 c         1048 g          417 t
ORIGIN      
        1 tcctgatcct gctcgttctc ggaggcttcg tggcgctcgg cgcgggcctc cggtcggcgc
       61 aggacgcgca acagcgcagg caagaggtgc agcagttcgc gtcgcaggtc gacgcggcgt
      121 tcgcccggag cggcatcgtc cagcagatcg ggccgacgcc cgtccccatc ccggagatcc
      181 aatcggcgat cgcggacctc gagaggcgca agctcgaccc gcagaggctc cgagccgacg
      241 ccgctcgatg gggcgagggg gcgtcggagc tctccgactc gatcgcggcg ttgggcgccg
      301 agtccccggc actgcgggaa gcacgcgatc tcatgcggca atcactccgg ctgtacgcct
      361 cgatcgcgca ggagatccgc gtcgcggcga ccctcgaccc gggggagcgg agcgagctgg
      421 tcggtcccct ccagcagcag atgcagcttg ccggctcgtt cttcgacttc ggatggaggt
      481 cgctcgcgac ggagcgcgag ctggccggga tcccggtcgt ggaggagccg ctcgccccgc
      541 cggggttcca ggcgccgtag catgcgcgtc cacgccgacg ggtcgctcga agtcgccgcg
      601 ccatgacggt cctgctgctg gtccacgcgt tccccgtcga cgcgtcgatg tgggagccgc
      661 agatcaccgg gctcggcggt gatctccagg tcctcgcgcc gtcgctcccc gggttcggtg
      721 gggagagccc gccgccgggc gcggtcctga cgatggacgc cgcggccgac gcgatgggcg
      781 aggcactcga ccgtgcgggc gtccagtccg ccgtgatctg cgggctgtcg atgggcgggt
      841 acgtcgcgtt ctcgatgtgg cggcggcacc gagacaggat cgacgccttg gtgctcgccg
      901 acacgcgcgc ggagcccgac gacgacgccg ggcgggagcg gcgacggacc gtcgccgaga
      961 ccgcgcggac gcgggggagc gcggcgatcg cggagaaccc tccggcgctc ctctcggacg
     1021 gcgccgacga cgccctctgg gaccgcgtga agggatggat ccgggcgcag cctggcgagg
     1081 cgatcgccgc tgcgtcgctg ggcatggcgg agcgcccgga ttcgcgcccc atcctcggcg
     1141 agatcgacgt tcccacgacg atcgtcgtcg ggtccgccga cgcgctcacg ccgccggcga
     1201 tgtcggagtc gatggccgag gcgatcccgg gcgcccagct cgtcgtgctc gacggtgcgg
     1261 gccacctgtc gaacctcgag gatcccgagg ggttcaacgc cgcgatcgag gggatcctcg
     1321 accgcgtcgg gcgcgagggg atgcagcggc cgtagcgcct ccctcagccg cccgcgtcgg
     1381 gtggaaaaat cctcggtgat ggacctcacg acgttcgccg accgataccc gttccccctc
     1441 gacgcgttcc agcggggggc gatcgacgcg ctcgccgcgg gggaatcggt gctggtcgcc
     1501 gcgccgacgg gatccgggaa gaccgtcgtc gcggagtacg cgatcgagca ggcgctcgcg
     1561 cgtggacgga aggcgttcta cacgacgccg ctgaaggcgc tctcgaacca gaagttcggc
     1621 gacttcgtcg ctcgctacgg cgcagatgag gtcgggctcc tcacgggcga caacagcatc
     1681 aacggcgagg ctcccgtcgt cgtgatgacg accgaggtgc tgcgcaacat gctgtacgag
     1741 cagagcgaca cgctcgacga cctcgaggtc gtcgcgatgg acgaggtcca ttacctccag
     1801 gacccgtatc gcggggcggt atgggaggag gtcctgatcc acctgccgct gtcggtgtcg
     1861 gtcgtctgtc tctccgccac ggtgtcgaac gcggaggagt tcggtgagtg gctcacgacg
     1921 ctgcggggca cgacccgtgt cgtcatcgag gagaagcggc cggtgccgct cgagcagctc
     1981 tacatggccg ggcgggagct gcacccgatg tacgtggagc gccggggcgc accgatgccc
     2041 aacccagaag tcgccgcgct gacgaggcgc gagcgcagga tcggcgagtc acgaggacgg
     2101 gcccccgaag gccggcagcc ctcccgcgcg gcgaggtcgc agggcgaagg ccgccgcctc
     2161 tacgtgccgc gacgcgagga cgtcgtcgaa gtgctcggcg atcaggggat gcttcccgcg
     2221 atctacttcg tgttctcgcg tgcggggtgc aaccgaagcg tcgagttcct tatggagtcg
     2281 ggcctgcggc tcaccgacgc gaacgaggag cgtcgcatcc gcgagctcgt cgagctgcgt
     2341 gcggcctgga tcgaggacga cgacctcgcg gcgctcgggt tcgccacgtt ccgcgaggcg
     2401 ctcgcggccg gcatcgcggc ccaccatgcg ggcatggtcc ccgtattcaa ggagaccgtc
     2461 gaggagctgt tcgcggcggg cctcgtgaag gtcgtcttcg cgaccgagac gctgtcgctc
     2521 ggcatcaaca tgccggcgag gagcgtcgtg atcgagagcc tctggaagtt ctcgggcgag
     2581 cggcacgagc tcctcacgcc gggcgagtac acgcagctta ccggccgagc cggccggcgc
     2641 ggcatcgacc cgcacggcta cgcggtcgtg ctgcatcaac gcgacgtcga cttcgagcgc
     2701 gtcgccggcc tcgcgaccac gcggacgtac gagctccgtt cgtcgttccg cccttcgtac
     2761 aacatgaccg tgaacctcgt ccggacgtac acgcccgagg agacccatca cctcctgaac
     2821 tcgtcgttcg ctcagttcca ggccgaccgc ggggtcgtgg gg
//
LOCUS       DASZOB010000536         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_18975, whole genome shotgun sequence.
ACCESSION   DASZOB010000536 DASZOB010000000
VERSION     DASZOB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18975"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VG709_08425"
     CDS             complement(<1..382)
                     /locus_tag="VG709_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWC33138.1"
                     /translation="MDSTNVGTLVRMADADLTVADPADDVRGRTVVDATGDEIGDVKS
                     LLIDEDERKVRFLEVESGGFLGLGSETRLVPVDAVTRVEENAVHVDQTRESVHGSPAY
                     DPELEQDAAYYGEVYSYYRYPPYWA"
     gene            complement(535..1224)
                     /locus_tag="VG709_08430"
     CDS             complement(535..1224)
                     /locus_tag="VG709_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWC33139.1"
                     /translation="MPGRILIVDDDEGIRDVVGFALENAGFEVEAVEDGETALEAATA
                     NPYDAVVLDVMMPGVSGIDVCRELRARSGVPILMLTAKDAEVERVLGLELGADDYVTK
                     PFSTAELVSRIRAVVRRRELDRSENRSAIREVGGLRIDFDRHEVVADGRPVSLTLSEF
                     RLLALLAESPDRVYTRRQIMQHLWRSDFVGDEHACDVHVSNLRRKIERSPARPERLLT
                     VRGLGYKLASA"
     gene            complement(1251..1490)
                     /locus_tag="VG709_08435"
                     /pseudo
     CDS             complement(1251..1490)
                     /locus_tag="VG709_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT          227 a          527 c          505 g          250 t
ORIGIN      
        1 gcgcccagta cggtgggtag cggtagtagc tgtagacctc tccgtagtac gccgcgtcct
       61 gctcgagctc gggatcgtac gccggcgagc cgtggacgct ctcgcgagtc tggtcaacgt
      121 gcaccgcatt ttcctcgacc cgcgtgaccg cgtcgacggg aacgaggcgc gtctcgcttc
      181 ccaacccgag gaagccgccg ctctccacct cgaggaaccg gaccttccgc tcgtcctcgt
      241 cgatcaggag cgacttcacg tcgccgatct cgtccccggt cgcgtcgacc acggtccgcc
      301 cacggacgtc gtcggcggga tcggcgacgg tcaagtcggc atcggccatt cgcaccagtg
      361 ttccgacgtt agtggagtcc attctccctc cttccgctcg ttgggcgtcg taccattccg
      421 ctttcgacga gcggtctacc cgccggactt cacgcgacct gcggggtcgc atagctgatc
      481 gtcagtgcac cgtcagcgtt cgctgaagca ccgtccggtc gaacgacgtg gcctctaggc
      541 cgaagctagc ttgtagccga ggccgcgaac tgtgaggagt ctctccggcc gcgcggggct
      601 gcgctcgatc tttcgccgga ggttcgagac gtggacgtcg caggcgtgct cgtcgccgac
      661 gaagtcgctt cgccagaggt gctgcatgat ctgccggcgc gtgtaaacac ggtcgggcga
      721 ttccgcgagc agcgcgagga gccggaactc cgagagcgtc agagagacgg ggcgcccgtc
      781 cgcgacgacc tcgtgacggt cgaagtcgat gcgaagcccg ccgacctccc tgatcgcgct
      841 cctgttctcc gagcggtcga gctcacgccg ccggacgaca gcccggatac ggctcacgag
      901 ctcggccgtc gagaagggtt tcgtcacgta gtcgtcggcg ccgagctcga ggccgaggac
      961 gcgctcgacc tcggcgtcct tggcggtgag catgaggatc ggcacgccgc tccgcgcgcg
     1021 cagctcgcgg cagacgtcga tgccggacac accgggcatc atcacgtcga gcacgaccgc
     1081 gtcgtacggg ttcgccgttg cggcctcgag cgccgtctcc ccgtcctcga ccgcctcgac
     1141 ctcgaagccc gcgttctcga gcgcgaagcc gacgacgtcg cgaatccctt cgtcgtcgtc
     1201 gacgatgagg atccgacccg gcatgcgcgg tcccggtggg cggccggaag gacgatgcga
     1261 accgtcgtgc cgacgttcgg ctccgaggca aactcgaggc ggccaccgag cgcccgcgcg
     1321 atctcccgta cgattgagag cccgaggccg aagccgtcgc cgttgcgcct cgagccgcgg
     1381 tagaagcgat cgaagatccg ctcctcgtgc tcgcccgaga tcccggggcc ggtgtcggcg
     1441 acgtcgatcg cgacgtctcc cgaagcgagc gggatggcgc ggagcgcgat cgtgccagcg
     1501 ttcgtatgc
//
LOCUS       DASZOB010000537         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_18994, whole genome shotgun sequence.
ACCESSION   DASZOB010000537 DASZOB010000000
VERSION     DASZOB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18994"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VG709_08440"
     CDS             complement(<1..317)
                     /locus_tag="VG709_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWC33140.1"
                     /translation="MHLLLVDDDPGLRALLRTTFEVVDIDLDEAGSALEAEALLEERR
                     PDAIVLDVSMPGMSGLDLCRRLKARPETRDIPVGLLTGSEMGTEEDSKAAGANAFVRK
                     PFSP"
     gene            complement(406..1239)
                     /locus_tag="VG709_08445"
     CDS             complement(406..1239)
                     /locus_tag="VG709_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWC33141.1"
                     /translation="MTFEHVLVVGAGQMGRGIAQVMAASGRRVSLHDAIPGATERATA
                     AMRKSLEKLVAKGEHVDPDAVVERVEPVGDLVEADLMVEAVVEDAAVKADIFARADET
                     LPPDAILASNTSSIPIATLAAATGRPDRVIGMHFFNPVPVMSLVEVIRTPATSEETAR
                     AVVDLARELGKTPAEANDFPGFVSNRILMPFINEAAYALQDGVAEAEAIDTIAKLGFK
                     HPMGPLALADLIGLDTCVSIMEVLRAGLEDAKYEPCPLLRRHVAAGRLGRKTGHGFYV
                     Y"
     gene            complement(1268..>1934)
                     /locus_tag="VG709_08450"
     CDS             complement(1268..>1934)
                     /locus_tag="VG709_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33142.1"
                     /translation="RGTVDKRVERAMRGLIAICVGLGLAAAAAGCGPGGDEAEPRAAR
                     TDASDAIEEAKKRLVPPPIFLASRAGRQRAVPGKSCVLTVGQGRGEGAASCYYPPERV
                     RPRVLNVVGPGERVGLVIPKTEIVERVGCVGRHECGGWTTVRPLGCRREVAVFRIGGA
                     RTTWEANLSSGAYELDVSIDFRTEDDLTGDAEGVVGLLVDPARTPTIVPFRPGLARCP
                     AGS"
BASE COUNT          286 a          705 c          654 g          289 t
ORIGIN      
        1 gggctgaagg gcttgcggac gaacgcgttg gcgcccgccg ccttgctgtc ctcctcggtt
       61 cccatctccg agcccgtgag gaggccgacg gggatgtcgc gcgtctctgg ccgagccttg
      121 agccggcggc agaggtcgag cccgctcatc cccggcatgc tcacgtcgag gacgatcgcg
      181 tccggcctcc gctcctccag gagtgcctcg gcctcgagcg cgctcccggc ctcgtcgagg
      241 tcgatgtcga ccacctcgaa cgtcgtccgg aggagggcgc gaagcccagg atcgtcgtcg
      301 acaagcagga gatgcacgga tcgtcctcgg ttttagggaa aaacggtttt cttatcgggc
      361 cggcgccgga tttcctttag ggccgccgca gagaaaaccc tgcccctaat agacgtaaaa
      421 accgtgcccg gtcttgcggc ctagtcggcc ggcggcgacg tgcctgcgaa ggagcggaca
      481 gggctcgtac ttcgcgtctt cgagccccgc gcgcagcacc tccatgatcg acacgcacgt
      541 gtcgagcccg atcaggtcgg cgagggcgag cgggcccatc gggtgcttga agccaagctt
      601 cgcgatcgtg tcgatcgcct cagcttcggc cacgccgtcc tggagcgcgt acgcggcctc
      661 gttgatgaag ggcatcagga tccggttcga gacgaagccg ggaaagtcgt tcgcctccgc
      721 cggcgtcttc ccgagctcgc gggcgagatc gacgaccgcg cgcgccgtct cctccgacgt
      781 cgccggcgtc cgaatcacct ctacgaggga catcacggga accgggttga agaagtgcat
      841 cccgatcacg cggtccggcc ggccggtcgc cgccgcaagc gtggcgatgg ggatcgacga
      901 cgtgttcgag gcgaggatcg cgtccggcgg aagggtctcg tcggcgcggg cgaagatgtc
      961 cgccttgacg gcggcatcct cgacgacggc ctcgaccatc aggtccgcct cgacgaggtc
     1021 cccgaccggc tcgacacgtt cgacgacggc gtccggatcg acgtgctcgc ctttcgcaac
     1081 gagcttctcg aggctttttc gcatcgccgc ggtagcccgc tcggtcgccc ccggaatggc
     1141 gtcgtgaagc gaaacgcgcc ggccggaagc cgccatgacc tgcgcgatcc cacgtcccat
     1201 ctgcccggcg ccgacgacga gaacgtgctc gaacgtcacc ggcccagtct aagccgcctc
     1261 gaacgggcta cgagccggcc gggcagcgag caaggccagg gcgaaacggc acgatggttg
     1321 gcgtccgagc cgggtcgacg aggagcccga cgacgccctc cgcgtcgccc gtcagatcgt
     1381 cctcggtcct gaagtcgatc gacacgtcga gctcgtaggc gcccgacgaa aggttcgcct
     1441 cccaggtcgt ccgtgcgccg ccgattcgga agaccgcgac ttcgcggcgg cacccgagcg
     1501 ggcgcacggt cgtccaccca ccgcactcgt ggcggccgac gcagccgacg cgctcgacga
     1561 tctccgtctt cgggatgacg aggccgacgc gctcgcccgg cccgacgacg ttcagcaccc
     1621 gaggccgcac gcgctccggc gggtagtagc agctcgccgc gccctcgcct cttccttggc
     1681 cgacggtcag cacgcagctc ttgcccggga cggcgcgctg gcggcccgca cggctcgcga
     1741 ggaagatcgg cggcggaacg agccgcttct tcgcctcctc gattgcgtcg ctcgcgtctg
     1801 tccgcgccgc gcgcggctcc gcctcgtcgc cgccgggccc gcagccggcc gccgccgcgg
     1861 cgaggccgag cccgacgcag atcgcgatca gcccgcgcat cgcgcgctcc actcgcttgt
     1921 cgacggtccc gcgc
//
LOCUS       DASZOB010000538         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_141094, whole genome shotgun sequence.
ACCESSION   DASZOB010000538 DASZOB010000000
VERSION     DASZOB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141094"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..731
                     /locus_tag="VG709_08455"
     CDS             54..731
                     /locus_tag="VG709_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33143.1"
                     /translation="MDLDVETAAASEGSVGFRRWLGALVGVTALVVALLSSLESDAGR
                     RAEEALVRASRLSLDIFGRIAASGPPQAFAAASAQEALAIGTAAQARIIAVFEERRAA
                     AASQELSQAEERVGRRLARVVEAMTAPPDPGSALDETTRTGVTTTIRDLEELLARQNA
                     QVDVAERYGTRQERAMFALILTAVAAVLLGVAGLTGERRAGRSAMVLAAGALVVAVGW
                     GASGFVV"
     gene            779..1044
                     /gene="ffs"
                     /locus_tag="VG709_08460"
     ncRNA           779..1044
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VG709_08460"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1082..2857
                     /gene="dnaX"
                     /locus_tag="VG709_08465"
     CDS             1082..2857
                     /gene="dnaX"
                     /locus_tag="VG709_08465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWC33144.1"
                     /translation="MEEYRALYRKYRPQSPADVLGQEHVVRALAGAVKEGRLAHAFLF
                     SGPRGTGKTSSARILAKMVNCDRGPTAEPCGVCEQCVRIREGTHLDVMEMDAASHGSV
                     DHARELRARAPPAPVQGREKVYIVDEAQRLSREAFDALLKLLEEPPPGVRFVLATTEP
                     HKMPPTIVGRTQRFEFRRIATETIAEQLQKVAAEESISLAAAAAESIARGSEGSLRDA
                     LSLLDQASVLGGGDVTEDVVVRLVGEPESEVLFGLADAVAVGDAKGVFGTIHRLVQDG
                     HDLRHFATQVTGHFRDLLLANASPDDTAIIDVAPELHPRLVAQAAKFSIGELDRILAL
                     LLQAQTDLRWSTAPRLTIELALVRAAMPETDPQPAAVLARLERLERLAGLPPSGGARD
                     VPDEVITQPGKETRGSPEEVVGNRREETRGSSEEVVAQRAGGERASPEAEPSGPTETA
                     VADGAEEPSGSQAHPHAPTTSAVTAEMLRRSWPQVVEALRAKRRMIASANAEVATVGA
                     FEDDVLELVCPPGREFAARKLEEKQSEVQAALAEVFGVSPKLRFTVRAGTVPEADLDE
                     PPPTPEAAEELVRRQFGAEVVAPEE"
     gene            2902..3495
                     /gene="recR"
                     /locus_tag="VG709_08470"
     CDS             2902..3495
                     /gene="recR"
                     /locus_tag="VG709_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWC33145.1"
                     /translation="MYEGPIQDLIDELNRLPGIGPKSAQRLAFHIVKSRAEDAHRLSD
                     AILQAKERVRFCRECYGVAEGELCRYCADPTRDTTVLCVVEEPKDVSSLERAGVMRGR
                     YHVLGGAISPMQGIGPDDLRVQELLQRVGQNGLREIVLATNPNLEGNATAMYVAGLLK
                     PLGVRVTRLASGLPVGGDLEYADEVTLGQALEGRREM"
     gene            complement(3499..3849)
                     /locus_tag="VG709_08475"
     CDS             complement(3499..3849)
                     /locus_tag="VG709_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33146.1"
                     /translation="MPKKLSVIVLLSATALAASACAHPEEEATIGERHEYRLLVHCGL
                     SVPTRFDGRWWWPVDPDLRETIRPPNGFEPSALHDEGHITLVSEDRALYESSQGRRIP
                     FEPTDVSGAAVGCE"
BASE COUNT          571 a         1373 c         1484 g          529 t
ORIGIN      
        1 ttcttcctcg cgctggcggt ggcgtgggtc gtcgccgtcg aggtggtggt ttgatggatc
       61 tcgacgtcga gaccgccgcc gcgagcgaag ggtccgtcgg gttccggcgg tggctcggcg
      121 cgctcgtcgg cgtcacggct ctcgtcgtcg cgctgctcag ctcgctcgag tccgacgccg
      181 ggaggcgggc ggaggaggcg ctcgtccgcg cgtcgcggct gtcgctcgac atcttcgggc
      241 ggatcgcggc ctcggggccg ccgcaggcgt tcgccgccgc gtcggcgcag gaggcgctcg
      301 cgatcgggac ggcggcgcag gcgcgcatca tcgcggtgtt cgaggagcgg cgggccgcgg
      361 cggcgtcgca ggagctctcg caggcggagg agcgtgtcgg gaggcgcctc gctcgggtcg
      421 tcgaagcgat gaccgctccg ccggatcccg ggtcggccct cgacgagacc acgcgaaccg
      481 gcgtgacgac cacgatccgc gacctcgagg agctcctcgc gcggcagaac gcgcaggtcg
      541 acgtggcaga gcggtacggc acacgccagg agcgggcgat gttcgcgctg atcctgacgg
      601 ccgtggcggc cgtgctgctc ggcgtcgccg gcctgacggg cgagcgacgc gccggccgga
      661 gcgcgatggt gctcgccgcc ggggcgctcg tcgtcgccgt cggctggggc gccagcggct
      721 tcgtggtcta gcgcccgccg ggcgcgtcgg accccgggtg tagactcgtt ctcggtgacc
      781 gcgctaggcg gggagctagc ggtgccctgt acccgcaatc cgctctagcg gggccgaagt
      841 ccacccgagg ccgtggcggt tcggagccgt cccgggtgcg gggcgatgaa ggccgggtcc
      901 cgcgcgacgc cgagccgtga accccgtgag gcccggaagg gagcagcggt aagcggaacc
      961 cggcgggtgc cgcgggggcg cctggctgga gccaccggcc cgggcaacgt ccgggtcgca
     1021 gcggtcgaag gtgggcacgc gcggtcaccg cttccctctt ccccccgaga cggaagggca
     1081 cgtggaggag taccgggcgc tctaccgcaa gtaccggccg cagtcgccgg ccgacgtcct
     1141 cggacaggag cacgtcgtcc gcgcgctcgc cggcgccgtg aaggaaggcc gcctcgcaca
     1201 cgcgttcctg ttctcggggc ctcgcgggac cggcaagacg tcgtcggcgc ggatcctcgc
     1261 gaagatggtg aactgcgacc gcggcccgac ggccgagccg tgcggcgtct gcgaacaatg
     1321 cgtccggatc cgggagggca cgcacctcga cgtgatggag atggacgcgg cgtcgcacgg
     1381 gagcgtcgac cacgcgcgcg agcttcgcgc gcgcgcgccg cccgccccgg tccaggggcg
     1441 cgagaaggtc tacatcgtcg acgaggcgca gcggctctca cgtgaggcgt tcgacgcgct
     1501 actcaagctc ctcgaggaac ctccgccggg cgtgcgcttc gtcctcgcga cgaccgagcc
     1561 gcacaagatg ccgccgacga tcgtcggacg gacgcagcgc ttcgagttcc ggcgcatcgc
     1621 gaccgagacg atcgccgagc agctccagaa ggtggccgcc gaggagtcga tctcgctcgc
     1681 ggccgcggcc gcggagtcga tcgcgcgcgg gtcggagggg tcgttgcgcg acgcgctctc
     1741 gcttctcgac caggcctcgg tgctcggcgg cggagacgtc accgaggacg tcgtcgttcg
     1801 gctcgtcggc gagccggagt cggaggtgct cttcgggctg gcggacgccg tcgccgtggg
     1861 cgacgcgaag ggggtcttcg ggaccatcca tcgcctcgta caagacggcc acgacctccg
     1921 gcacttcgcc acgcaggtga ccggtcactt ccgcgacctc ctgctcgcga acgcctcgcc
     1981 cgacgacacc gcgatcatcg acgtcgcgcc ggagctccat ccacggctcg tcgcgcaggc
     2041 ggcgaagttc tcgatcggag agctggaccg gatcctcgcg ctcctgctcc aagcacagac
     2101 cgaccttcgg tggtcgacgg cgccgcgcct cacgatcgag ctcgcgctcg tccgcgccgc
     2161 gatgcccgag accgatccgc agcccgcggc ggtgctggcg cgcctcgagc gcctcgaacg
     2221 cctcgccggc ctcccgccgt caggtggggc gcgagatgtg cccgacgagg taatcacgca
     2281 gccagggaag gagacgcgag gctcgcccga ggaggtcgtc gggaaccggc gggaggagac
     2341 gcgaggttcg tccgaggagg tagtcgcgca gcgagccgga ggcgagcgag catcccccga
     2401 agccgagccg agcggcccaa cggagacggc cgtcgcggac ggtgcggagg aaccgagcgg
     2461 ctcacaagcg cacccgcacg cgccgacgac gtccgcggtg accgcggaga tgctgcgccg
     2521 ctcatggccg caggtcgtcg aggcgctgcg cgcgaagcgc cggatgatcg cctcggccaa
     2581 cgccgaggtg gcgaccgtcg gcgcgttcga ggacgacgtc ctcgagctcg tctgcccgcc
     2641 ggggcgcgag ttcgccgcgc ggaagctcga ggaaaagcag agcgaggtcc aggcggcgct
     2701 cgccgaggtc ttcggggtct cgccgaagct ccgcttcacg gtccgggccg gcacggtccc
     2761 cgaggccgac ctcgacgagc cgccgcccac gccggaggcc gccgaggagc tcgtccgccg
     2821 acagttcggg gcggaggtcg tcgctccgga ggagtaggat tacgccgatg tgattcggtg
     2881 gccggcgcgg gcggaggcgc ggtgtacgag ggaccgatcc aggacctgat cgacgagctg
     2941 aaccgtctcc ccggcatcgg gccgaagtcg gcgcagcgcc tcgccttcca catcgtgaag
     3001 tcgcgggccg aggacgctca tcggctttcc gacgcgatcc tgcaggccaa ggaacgcgtg
     3061 cggttctgcc gcgagtgcta cggcgtcgcc gagggcgagc tctgccggta ctgcgcggac
     3121 ccgacgcggg acacaaccgt cctctgcgtc gtcgaggagc cgaaggacgt gtcgtcgctc
     3181 gagcgcgccg gcgtcatgcg cggccgctac cacgtgctgg gtggggcgat ctcgccgatg
     3241 caggggatcg gccccgacga cctccgcgtg caggagctcc tccagcgcgt cgggcagaac
     3301 gggctgcgcg agatcgtgct ggcgacgaat ccgaacctcg agggcaacgc gaccgcgatg
     3361 tacgtcgcgg ggctcctcaa gccgctcggc gtgcgcgtca cgcgcctcgc ctcgggactc
     3421 ccggtcggcg gggacctcga gtacgccgac gaggtcacgc tcggccaggc gctcgagggc
     3481 cgccgcgaga tgtaacggct actcgcaacc gacggccgcc cccgatacgt ccgtcggctc
     3541 gaacgggatc cgtcgaccct gcgaggactc gtacagagcg cgatcctcgg acacgagcgt
     3601 gatgtgaccc tcgtcgtgga gggcggacgg ctcgaaccca tttggcgggc ggatggtctc
     3661 tctcaggtcc gggtcgaccg gccaccacca acgcccgtcg aaccgtgtcg ggacggaaag
     3721 gccacagtgg accagcagcc ggtattcgtg cctctcacca atcgttgcct cttcctcggg
     3781 atgagcgcac gccgaggcgg ccagcgcggt agccgaaagg agcacgatga ccgacagctt
     3841 ctttggcatg gagaaacaca taagaggagg agaaacccct tgacaagcca tcggtcgcga
     3901 cagccgtgag gtaacggttt ccgcagcgcc tcggtcgcat cgtcgtggcc ctcactc
//
LOCUS       DASZOB010000539         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_19064, whole genome shotgun sequence.
ACCESSION   DASZOB010000539 DASZOB010000000
VERSION     DASZOB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19064"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VG709_08480"
     CDS             <1..447
                     /locus_tag="VG709_08480"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33147.1"
                     /translation="RLKRAAAVEEVFPEGTLGRRKNVYRVTAKGENLFAELVESAGNH
                     EGVGDDNGFRVRLAFFRYLKPETRIGVLERRRAALSNRLAELRKRLTAYRERVDGYTL
                     SLMRHGMDATERDILWVDGMIEAERRAARPSRRVRKRAAVGKQEGS"
     gene            444..1568
                     /locus_tag="VG709_08485"
     CDS             444..1568
                     /locus_tag="VG709_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018221916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWC33148.1"
                     /translation="MSKVRLAIVGVGNCASSLVQGLHFYRDADPAERVPGLMHVRLGP
                     YHVSDVEVVAAFDVDGKKVGRDVAEAIFAEPNNTVRFSDVPPTGVQVLRGPTFDGLGH
                     YYREAIEESDDEPVDVAEVLRETQADVVINYLPVGSEQAARHYARAAIDAGAGFVNCM
                     PVFIASDPTWARAFADAGLPIVGDDIKSQVGATIVHRVLAKLFEDRGVILDHTSQLNF
                     GGNMDFMNMLERERLTSKKISKTRAVTSQLRDGIGPDDIHIGPSDHVPWLKDRKWAHI
                     RLEGREFGDVPMSVELKLEGWDSPNSAGVAIDAVRCVKLANDRGVVGPLIGPSAYFMK
                     SPPVQFADDAARQMVEDFILGNGDSARLDWSRFFDTQPQG"
BASE COUNT          263 a          558 c          574 g          245 t
ORIGIN      
        1 cggctgaagc gcgcggccgc cgtcgaggag gtcttcccgg aagggacgct gggacggcgg
       61 aagaacgtct accgcgtgac cgcgaagggc gagaacctct tcgcggagct ggtcgagtcc
      121 gcgggcaacc acgagggcgt cggcgacgac aacggcttcc gcgtccggct ggcgttcttc
      181 cggtacctga agccggagac gcggatcggc gtcctcgagc gtcgccgcgc cgcgctgtcg
      241 aaccggctcg cggagctccg caagcgcctg acggcgtacc gggagcgggt cgatgggtac
      301 acgttgtcgt tgatgcgcca cgggatggac gccaccgagc gggacatcct gtgggttgac
      361 gggatgatcg aggcggagcg acgagccgcg cgcccaagcc ggcgcgtccg caagcgggcc
      421 gccgtgggga agcaggaggg atcatgagca aggttcgcct cgcgatcgtc ggggtcggta
      481 actgcgcctc gtcgctggtt cagggcctgc acttctaccg cgacgccgac cccgccgagc
      541 gcgtgccggg gctcatgcac gtccgtctcg gtccgtacca cgtctccgac gtcgaggtcg
      601 tcgcggcgtt cgacgtcgac ggcaagaagg tcggccggga cgtcgccgag gcgatcttcg
      661 cggaacccaa caacaccgtg aggttctccg acgtcccgcc gaccggcgtg caggtcctcc
      721 gcggcccgac gttcgacggc ctcggtcact actatcggga ggcgatcgag gaatccgacg
      781 acgagcctgt cgatgtcgcc gaggtgctcc gggaaacgca ggcagacgtc gtcatcaact
      841 acctcccggt cgggagcgag caggccgcac gccactacgc gcgcgcggcg atcgacgcag
      901 gtgccggctt cgtgaactgc atgccggtgt tcatagcgtc ggatccgacg tgggctcgcg
      961 cgttcgcgga cgccggcctg ccgatcgtcg gcgacgacat caagtcccag gtcggcgcga
     1021 cgatcgtcca ccgggtgctc gcgaaactct tcgaggacag gggcgtgatc ctcgaccaca
     1081 cgtcccagct caacttcggc ggcaacatgg acttcatgaa catgctcgag cgcgagcggc
     1141 tcacgtcgaa gaagatctcg aagacgcgcg cggtcacgtc gcagctgcgc gacggcatcg
     1201 ggcccgacga catccacatc ggtccgtcgg accacgtccc gtggctcaag gaccggaagt
     1261 gggcccacat ccgactcgag ggacgcgagt tcggggacgt cccgatgtcg gtcgagctga
     1321 agctcgaggg ctgggactcg ccgaactccg ccggcgtcgc gatcgacgcg gtccgctgcg
     1381 tgaagctcgc gaacgaccgc ggcgtcgtcg gcccgctgat cggcccgtcg gcgtacttca
     1441 tgaagtcacc accggtccag ttcgccgacg acgcggcccg ccagatggtc gaggacttta
     1501 tcctcggtaa cggcgactcc gcccggctcg actggtcgcg cttcttcgac acgcagcccc
     1561 agggctgagg tcgcctacgc ttcacgcgtg aacgccgctc cgggtagcgg ccggctcgac
     1621 acgctccgcg tcctcgtgcg
//
LOCUS       DASZOB010000540         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_19095, whole genome shotgun sequence.
ACCESSION   DASZOB010000540 DASZOB010000000
VERSION     DASZOB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19095"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1387)
                     /locus_tag="VG709_08490"
     CDS             complement(104..1387)
                     /locus_tag="VG709_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33149.1"
                     /translation="MRRTARTAAILVLVLAGSLVPSAPIAVAHQIPSVPVGFAVTPYT
                     QTRGLTTSIAFGPDTRDPSRERLYAAVFSSGQIAAIDDAGGVGTVSTFASGFRNPLGV
                     VGAPDGTVFVADSEAAREGPFGTRAYGRVWRVRDTNRDGVADVSEVVLKDLPNGRHNT
                     NGMALGPDGMLYVTNGNSTDDGVEGGDPEVEPWSGSVVRVDPSWTGVSLASLRPKHVL
                     VAHGMRNDFDVARSPFDPTQLFITMNGTDDARQESEEGPTGLEDSDDLLYRTDIDDTV
                     GPGRKGKPVIDDFGFPSCLYNLERRGNLDPYDNPNPAVIEQFGPCPTGTVPRPLTSFG
                     LHVSADGLAFQTTPAWGAEFRNDLFVAEFGNFFGSEIVGHRIVRVELDGKGQSVTRRG
                     GFLSGAVPLDVTFDPAGAMYVADFSGTIFKVQRVA"
     gene            complement(1401..2582)
                     /locus_tag="VG709_08495"
     CDS             complement(1401..2582)
                     /locus_tag="VG709_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWC33150.1"
                     /translation="MNRDRRYATRPALALAIALTSLSPALTAQPAMAATGSDEALARL
                     VDAFAGFQRAARPLVPGADIAAVMPRLSAARADLLDAAVRLGDVTSSGHAFSAAAPVQ
                     TPCLDLVGVDSTYTTNCALLVDVGGSDVYANNAGGVDGGAAALIDLGGNDRYGGTLNS
                     GANGGAVAGAGFLYDASGDDAYSAGSNGTNGGGYFFGSGFLLDGGGNDRYTATQSGTN
                     GGAHSVASGHLIDLGAGADSYSATRFGVNGGSTAGTGLLLDAGGTDTYSDQEACSASG
                     VDRTVVPTGMAGGQIDSPITRSSTCPPGVVQPPPRPAFGSVTGTVTDARGPGIVGAQV
                     QCGGAPPAVALADGRYTVPVASAGANRCTASAVGFRPKPVDLTVTEGATTVLNFALRR
                     A"
     gene            2658..3278
                     /locus_tag="VG709_08500"
     CDS             2658..3278
                     /locus_tag="VG709_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWC33151.1"
                     /translation="MDCHGIDFRGRRQSLAGAEGYLKVSGLSDESLLAGLRSGEPDHT
                     AAFVRRFQSRVYGLAMKVVGDTNVAEDVAQETFVRAWRYAGAYDARRGPVATWLLTIA
                     RNVATDARRLRGWECRDPAELAALGVPSPDAGPEETALALGEAEELRRAIAELPEGQR
                     RALVLAAFQGCTAREIGEAEGIALGTAKTRIRAAMMKLRSALEGRR"
     gene            3275..>3787
                     /locus_tag="VG709_08505"
     CDS             3275..>3787
                     /locus_tag="VG709_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWC33152.1"
                     /translation="MTRKHDEVRALLPEVALGVASGDDRAAVLEHVMGCADCRRDLEK
                     LSETADALLLLGPEMQPPAGFESRVVGRLGPTAARSPGRTRWRRALQLAAAAIIGAVA
                     VVAPYSAVKRDDAQLGALYRQTLERANGVAFLALPLEDPRGERAGHVYGYEGEPSWVL
                     VAVTSAQPGRY"
BASE COUNT          519 a         1314 c         1421 g          533 t
ORIGIN      
        1 acgcggacgc gaccgcgaac gacacagcga cgacggcgag cgcgaccgac cctcgtagga
       61 gggtgcgaag cggggtcgat gcgggcacct cgggctcctc tccctacgcg accctctgga
      121 ccttgaagat cgttccgctg aagtcggcga cgtacatcgc gccggccggg tcgaacgtca
      181 cgtcgagggg cacggccccg gacaggaagc cgccgcggcg cgtcacgctt tggcccttcc
      241 cgtcgagctc gacgcgcacg atgcggtgcc cgacgatctc gctcccgaag aagttgccga
      301 actccgcgac gaagaggtcg ttccggaact ccgcgcccca ggccggcgtc gtctggaacg
      361 cgagcccgtc ggcggagaca tggaggccga agctcgtgag gggccgcggg acggtcccgg
      421 tcgggcacgg cccgaactgc tcgatcaccg ccgggttcgg gttgtcgtag gggtcgaggt
      481 tgccgcggcg ctcgaggttg tacaagcacg aagggaaccc gaagtcgtcg atcacgggct
      541 tccccttcct gccgggcccg acggtgtcgt cgatgtcggt ccggtagagc aggtcgtcgg
      601 agtcctcgag gccggtcggg ccctcctcgg attcctgcct cgcgtcgtcg gtgccgttca
      661 tcgtgatgaa cagctgcgtc gggtcgaacg gcgagcgcgc gacgtcgaag tcgttgcgca
      721 tcccgtgtgc cacgagcacg tgcttcgggc ggagcgacgc gagcgagacg ccggtccacg
      781 acgggtcgac gcggacgacg gaccccgacc acggctccac ctccgggtcg ccgccctcga
      841 ctccgtcgtc ggtcgagttg ccgttggtga cgtagagcat cccgtccggc ccgagggcca
      901 tcccgttcgt gttgtgccgg ccgttcggaa gatccttcag cacgacctcg gagacatccg
      961 cgacgccgtc gcggttcgtg tcgcgcacac gccacacgcg gccgtacgcg cgcgtgccga
     1021 acggtccctc tcgcgcggcc tcggagtcgg cgacgaatac cgtcccgtcc ggcgccccca
     1081 cgaccccgag ggggttccgg aagcccgacg cgaatgtcga cacggtgccg acgccgcccg
     1141 cgtcgtcgat cgcggcgatc tgcccgctcg agaacaccgc cgcgtagagg cgctcgcgcg
     1201 acgggtcgcg ggtgtccgga ccgaatgcga tgctcgtcgt gagcccgcgc gtctgcgtgt
     1261 acggggtgac cgcgaacccc accgggacgg acgggatctg gtgcgcgacg gcgatcggag
     1321 ccgacggaac gagcgatccc gcgagaacga gcacgaggat cgcggcggtg cgggcagtac
     1381 gcctcatcgt tcctccttca ctaggccctt cgcagcgcga agttcaggac ggtcgtggcg
     1441 ccttcggtga ccgtgaggtc gacgggcttc ggccggaagc cgacggcgct ggcggtgcat
     1501 cggttcgccc cggcgctggc gacggggacg gtgtaccgcc cgtcggcgag ggcgaccgcg
     1561 ggcggcgctc cgccgcactg gacctgcgcg ccgacgatcc cgggtccgcg cgcgtcggtc
     1621 accgtaccgg tgaccgagcc gaacgcgggc cgcggcggcg gctggacgac cccaggcggg
     1681 cacgtcgagc tccgtgtgat cggcgagtcg atctgtccgc cggccatccc cgtcggaacg
     1741 acggtccggt cgacgcccga cgcactgcac gcttcctggt ccgagtacgt gtcggtccca
     1801 ccggcgtcga gcagcagacc cgttcccgcg gtcgagccgc cgttcacacc gaacctggtg
     1861 gccgagtacg agtccgcgcc cgcaccgagg tcgatcagat gtcccgatgc gacggagtgg
     1921 gcgccgccgt tcgtgccgga ctgcgtcgcg gtgtagcggt cgttcccgcc gccgtcgagg
     1981 aggaagccgg atccgaagaa gtagccgccg ccgttcgtgc cgttcgaccc ggcggaatac
     2041 gcgtcgtcgc ccgaggcgtc gtagaggaag cccgcgccgg cgacggctcc gccgttcgcc
     2101 ccgctgttga gcgtcccgcc gtatcggtcg ttgccgccga ggtcgatgag cgcggcagcg
     2161 ccgccgtcca cgcctccggc gttgttcgcg tagacgtcgc tgccgccgac gtcgacgaga
     2221 agcgcgcagt tcgtcgtgta cgtgctgtcg acgcctacga ggtccaggca cggcgtctgg
     2281 accggcgcgg cggcactgaa ggcgtggccc gacgacgtga cgtccccgag ccgaacggcc
     2341 gcgtcgagga gatcggctcg ggcggcgctc agccgcggca tcacagcggc gatgtcggcg
     2401 ccgggaacga gcggccgtgc cgcgcgctgg aacccggcga acgcgtcgac caggcgcgcg
     2461 agcgcttcgt cgctgcccgt ggccgccatc gccggctggg ccgtcagcgc cggcgagagc
     2521 gacgtgagcg cgatcgcgag agcgagggcg ggacgcgtgg cgtatcggcg gtcgcggttc
     2581 atcctctgcc tcctaacggc gatgccggcc ggcctttcgg ggcgggctcc cgtcggtaat
     2641 accggtcgtc tctccgcgtg gattgccacg ggatagattt ccggggccgt cggcaatccc
     2701 tcgccggcgc ggaggggtat ctcaaggtgt cgggcctgtc cgacgaatcg ctgctcgcgg
     2761 ggctgaggtc gggggagccc gaccataccg cggccttcgt gcgccgattc cagtcccgcg
     2821 tctacgggct cgcgatgaag gtcgtgggag acacgaacgt ggccgaagac gtggcgcagg
     2881 agaccttcgt ccgcgcgtgg cggtacgccg gcgcgtacga cgcgaggcgc ggccccgtcg
     2941 cgacgtggct gctgacgatc gctcggaacg tcgcgaccga cgcccgccgg ctgcgagggt
     3001 gggagtgcag ggatccggcg gagctcgccg cgctcggcgt ccccagcccg gacgcgggcc
     3061 cggaggagac ggccctcgcg ctcggcgagg ccgaggagct gcggcgcgcg atcgcggagc
     3121 tacctgaggg acagcggcgc gcgctggtgc tcgccgcctt ccagggctgc accgcccggg
     3181 agatcggcga ggccgagggg atcgcgctcg gcacggcgaa gacgcggatc cgagcggcga
     3241 tgatgaagct gcggtccgcg ctggagggac gacgatgacg cgaaagcacg acgaggtccg
     3301 tgccctcctt ccggaggtcg cgctcggcgt cgcctccggg gacgaccggg cggccgtgct
     3361 cgagcacgtc atgggatgtg ccgactgccg gcgcgacctc gagaagcttt ccgaaactgc
     3421 agacgcgctg ctgctcctgg gcccggagat gcagcctccg gcgggcttcg agtcccgggt
     3481 cgtcggccgg ctcggtccca ccgcggcgcg cagccccggc aggacgagat ggcgccgtgc
     3541 gctccagctg gcggccgcgg cgatcatcgg agccgtcgcg gtggtggcgc cgtactcggc
     3601 cgtcaagcga gacgatgctc aactgggagc gctgtaccgc cagacgctgg agcgggcgaa
     3661 cggcgtggcg ttcctcgcac ttccgctcga ggacccccgc ggggagcggg ccggccatgt
     3721 gtacgggtac gagggcgagc cgtcgtgggt gctggtcgcc gtgacgtcgg ctcaacctgg
     3781 gcggtac
//
LOCUS       DASZOB010000541         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_181335, whole genome shotgun sequence.
ACCESSION   DASZOB010000541 DASZOB010000000
VERSION     DASZOB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181335"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VG709_08510"
     CDS             <1..973
                     /locus_tag="VG709_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWC33153.1"
                     /translation="AAERALREAEEAARDAGATREEAARTAAEARRADAGANRAWREA
                     AAELQRLRDAYEDEEQARRDLERRIADAERAVAEGHRRDPAEAVAALDEADTPAELSR
                     RADLVARRLQLLGRVNLVAADEYRALQERHDFLKREIEDVQQARRDLQHVIRDVDRKI
                     VEIFDTAFRDVAAEFERTFATLFPEGEGKLVLTDPENLLATGVEIEARPGRKRVKRLS
                     LLSGGERALTALAFLFAIFRARPSPFYLLDEVEAALDDVNLHRFIQLVKGFAETSQVI
                     VVTHQKRTMEAADVLYGVSMGRDGSSSVISQRVSDAVTTDVVPADAM"
     gene            997..1968
                     /gene="ftsY"
                     /locus_tag="VG709_08515"
     CDS             997..1968
                     /gene="ftsY"
                     /locus_tag="VG709_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWC33154.1"
                     /translation="MPGGIALELVLVAALLVLLVVGFLLGRRWRKRPPPEPKPRREGL
                     GARVARLVGKDEPTDEDWRWLEEGLLRADAGPTAAREIVTRVRERYTPGDDPEDLLVG
                     EIAGMFERDDEFRLPRRMSVVMVVGVNGTGKTTTIGKLAHRLGREGRKVAVANSDTFR
                     AAAGEQLEVWAQRSGADLVATQQRGSDPGAVAFDAVEAAKARKRDVLVVDTAGRLHSK
                     QPLMAELSKVRRVIEKAAGRKPDEVLLVLDATTGQNGIAQARAFLDAVDVTGIALTKL
                     DGTAKGGTVLRIREELGVPVKLVGTGEAIDDLEPFDPKTFAESLVRG"
     gene            complement(2041..2436)
                     /locus_tag="VG709_08520"
     CDS             complement(2041..2436)
                     /locus_tag="VG709_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33155.1"
                     /translation="MDLPISVPAAMALAAVPVVVAVGVVLPIYLRRRRDPDFSPNVLN
                     GKPLRTRRAVSRALMLGEVPESPDDLDLASRLANSWRQRPWEQALALAAGTLAGAGAG
                     AFATFSWLRGGRPTALIGMVKAGATSAPA"
     gene            2789..>3098
                     /locus_tag="VG709_08525"
     CDS             2789..>3098
                     /locus_tag="VG709_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33156.1"
                     /translation="MITAFAAIGIDAGVVEHGYKDAKIGGRWRGTEIVVHAYPTRRAE
                     IHQRRTDVVGQTAIRGIPVLRATSTSFGRVLIVTCGERTYEVGGVDPSTLLPAYGDTV
                     E"
BASE COUNT          480 a         1059 c         1121 g          438 t
ORIGIN      
        1 ggccgccgag cgggcgctcc gcgaggccga ggaagcggca cgcgacgccg gggccacgcg
       61 agaggaggcc gctcggaccg cggcggaggc gcgccgcgcc gacgcgggcg cgaaccgcgc
      121 gtggcgggag gccgctgcgg agctccagcg tctgcgcgac gcgtacgagg acgaggagca
      181 ggcgcgacgg gatctcgagc gccggatcgc cgacgcggag cgcgcggtcg cggagggcca
      241 ccggcgagat ccggccgagg ccgtggccgc tctcgacgag gccgacaccc cggccgagct
      301 ctcgcggcgc gccgatctcg tcgcacgccg ccttcagctc ctcgggcgcg tgaacctcgt
      361 cgcggccgac gagtaccgcg cgctgcagga gcggcacgac ttcctcaagc gcgagatcga
      421 ggacgtccaa caggcgcgcc gcgatctcca gcatgtgatc cgcgacgtgg accgcaagat
      481 cgtcgagatc ttcgacacgg cgttccgcga cgtcgccgcg gagttcgagc ggaccttcgc
      541 gacgctgttc cccgagggcg agggcaagct cgtgctgacc gacccggaga acctgctcgc
      601 gaccggcgtc gagatcgagg cgcgccccgg caggaagcgt gtgaagcgcc tctcgctgct
      661 ctcgggcggc gaacgcgcgc tgacggcgct cgcgttcctg ttcgcgatct tccgcgcgcg
      721 gccgtcgccg ttctatctcc tcgacgaggt cgaggccgcg ctcgacgacg tcaacctcca
      781 ccgcttcatc cagctcgtga aggggttcgc cgagacgtcg caggtgatcg tcgtcacgca
      841 ccagaagcgg acgatggaag cggccgacgt tctctacggc gtctcgatgg ggcgcgacgg
      901 gtcgtcgagc gtcatatcgc aacgggtgtc ggacgcggtg acgaccgacg tcgtgccggc
      961 ggacgcgatg tagcgcggcg cgctagcgta gagaccatgc cgggcggcat cgccctcgag
     1021 ctcgtcctcg tcgcagcgct cctcgtgctg ctcgtcgtcg ggttcctgct cgggcggagg
     1081 tggcggaagc gcccgccgcc agagccgaag ccccggcgcg agggcctcgg cgcgcgcgta
     1141 gcgcggctgg tggggaagga cgagccgacc gacgaggact ggcggtggct cgaggagggg
     1201 ctgctgcggg ccgatgccgg ccccacggcc gcgcgcgaga tcgtcacgcg cgtccgcgag
     1261 cggtacacgc cgggcgacga ccctgaggat ctcctcgtcg gcgagatcgc ggggatgttc
     1321 gagcgggatg atgagttccg gctcccgagg cggatgtcgg tagtgatggt cgtcggcgta
     1381 aacggcacgg ggaagaccac gacgatcggg aagctcgcgc accggctcgg gcgcgagggt
     1441 cgcaaggtcg cggtcgcgaa cagcgacacg ttccgcgccg cggccggcga gcagctcgag
     1501 gtgtgggcgc agcgttccgg cgccgacctc gtcgcgacgc agcagcgcgg ctccgacccc
     1561 ggcgcggtcg cgttcgacgc ggtcgaagcc gcgaaggcgc gcaagcgcga cgtgctcgtg
     1621 gtcgacaccg ccggacgact ccactcgaaa cagccgctga tggccgagct ctccaaggtt
     1681 cgccgcgtca tcgagaaggc ggcgggccgc aagcccgacg aggtgctgct ggtcctcgac
     1741 gcgacgaccg gtcagaacgg catcgcgcag gcccgcgcct tcctcgacgc cgtcgatgtc
     1801 accgggatcg cgctcacgaa gctcgacggc accgcaaagg gcgggaccgt gctccgcatc
     1861 cgcgaggagc tcggggttcc ggtgaagctc gtcgggaccg gcgaggcgat cgacgatctc
     1921 gagcccttcg acccgaagac gttcgccgag tccctcgttc gcggctgatg cacttcgtac
     1981 tcggcttcgg cgcgttgatc gtcgttgccc tcgtgatcgc gatcgccgtc ggagggggcg
     2041 tcaggctggc gcggaagtcg cgccggcctt gaccatgccg atcagcgccg tcggccgccc
     2101 gccgcgcaac cacgagaacg tggcgaaggc accggcgcca gcgccagcga gagtccccgc
     2161 agccaatgcg agcgcctgct cccatggccg ctgcctccag ctgttcgcga ggcgtgaagc
     2221 gagatcgagg tcgtccggcg actccggaac ctcgccgagc atcagtgctc tcgagacggc
     2281 tctcctggtc ctgagcggct ttccgttgag gacgttcggg ctgaaatcgg gatctcgccg
     2341 ccgtcgtagg tagatcggta gcaccacccc cacggcgacg accaccggca ccgccgcgag
     2401 tgccatcgcc gccgggaccg agataggaag gtccaccagc cgattctgcc tcctgcgacg
     2461 cgtcgccgag cctgcctcgc ggtccgacca atccttcggc atgtgccgct gggccggcct
     2521 cgagcgctgc attggtaggc tccgcgctag gcacgagatg tttcgaaagc gtcgcaagct
     2581 cggtctcgta gctctcggtc tcgctctcgc cgcgatcgga gcgcaggcct gcgactcgcg
     2641 tgacgttacg gacttgggaa ggcctcagga aaccgtcccc gcggctgagt caccggctac
     2701 gccgggtgat tcgaaccgaa gctcgccgca gcccaagtgc tcaccgcccc cggaaacgcc
     2761 accgcctcct tcgcggatcg agcagcatgt gatcacggcg ttcgccgcga tagggatcga
     2821 cgcgggggtc gtcgagcatg gatacaagga cgcgaagatt ggcggccgtt ggcgtggaac
     2881 ggagatcgtc gttcacgcct acccgacgcg cagggctgag atccatcaga gacgaacaga
     2941 tgtcgtgggg cagacggcga tccgagggat ccccgtcctc cgtgcgacaa gcacgagttt
     3001 cgggcgcgtt ttgattgtga catgcggcga aagaacgtat gaggtcggcg gagtcgaccc
     3061 ctcgaccctt ctgcccgctt acggcgatac ggtggagc
//
LOCUS       DASZOB010000542         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_39402, whole genome shotgun sequence.
ACCESSION   DASZOB010000542 DASZOB010000000
VERSION     DASZOB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39402"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1234
                     /locus_tag="VG709_08530"
     CDS             98..1234
                     /locus_tag="VG709_08530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HWC33157.1"
                     /translation="MKAVVWHGKHDVRVDEVPDPKIEEPTDAVVRITSTAICGSDLHL
                     YSKLGLAMKKGDIIGHEPMGIVEEVGPDVSHIAGGDRVVIPFNVACGRCFMCRRGLQS
                     QCETTQNRRWGKGGSLFGYTHLYGGVAGGQAEYLRVPHAHYGPIKVPEGPPDVRFLFL
                     SDVLPTAWQAVEYADVPDDGTVVVWGLGPIGQLSARVARRRGAGRVIGVDSVAERRAM
                     AERWGVETIDAQDGDPVEAVRERTDGRGADAGIDAVGMEAEGSLLEPVLQGAKVVPDR
                     LHALKQTMGAVRRGATVSVSGVYTAWFPKFPLGQLFDRQISIRMGQANVRRWVDEIMP
                     LLGDDDPLGTDDLATHTVPLGEAPKMYERFQKKRDGTIKVVLRP"
     gene            complement(1252..1935)
                     /locus_tag="VG709_08535"
     CDS             complement(1252..1935)
                     /locus_tag="VG709_08535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWC33158.1"
                     /translation="MTIEGTIIVTGGASGLGAAVADAIQKTGAAAVVFDRMEPPSGAD
                     HEIVDLSDTERTEEAVRRVAERGDLSAVVTCAGTDSCGPLADVPRDDWERVVRVNLLG
                     TAAVVRAALPALERSRGRIVTVASTLGLRALPDATAYSASKFGVIGFTRALAAEMGGR
                     VGVTCLIPGGMATAFFDERPEKYRPAPDQNLASPSDVADAVVFALQQPRGLDVRELLI
                     MPADEPSWP"
     gene            complement(1932..>3042)
                     /locus_tag="VG709_08540"
     CDS             complement(1932..>3042)
                     /locus_tag="VG709_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWC33159.1"
                     /translation="VVVYTRRDDPTLPKRVKFAPGVTVEHVDAGPPKPLPKDELLPHM
                     DAFAAELEVAWRTWRPDVAHAHFWMSAKASVAAGRRTGVPIVLTFHALGVVKRRHQGA
                     KDTSPPERIAIEEALTQAVDHIVATSTDEVFELARLGTDPGRTSVIPCGVDLSLFRPD
                     GPTERKTPGLDRVVVVSRLVERKGIGNIVTAMGRVPGAELLVAGGPESGDLARDAEAR
                     RFLRLAEEAGVADRVRLLGRVERDQVPALLRSADLVACTPWYEPFGMVAVEAMACGVP
                     VVASAVGGLVDTVVEGVPGLHVRPRASDSIAEAIRRLLADPEIRAEMGRRGAARAAAQ
                     YGWDRIARLTLQVYTSVVERSAAAVARTTKHGRPS"
BASE COUNT          422 a         1077 c         1121 g          422 t
ORIGIN      
        1 ggcttcgtga gcgcgccggg ttcgacacgc cgccgctcgc gcgcgagacc ggcgggccga
       61 tgcggctacg cgaggtactg tcgggggtcg gcgcacgatg aaggccgtcg tctggcacgg
      121 aaagcacgac gtccgcgtcg acgaggttcc ggacccgaag atcgaggagc cgaccgacgc
      181 ggtcgtgcgg atcacctcga cggcgatctg cggctccgac ctccacctct actccaagct
      241 cgggctcgcg atgaagaagg gcgacatcat cgggcacgag ccgatgggga tcgtcgagga
      301 ggtcggcccg gacgtctcgc acatcgccgg aggggaccgc gtcgtgatcc cgttcaacgt
      361 cgcgtgcggt cgctgcttca tgtgccggcg cggtctccag tcgcagtgcg agacgactca
      421 gaaccgccgg tgggggaagg gcgggagcct gttcggctac acccatctct acggcggcgt
      481 cgccggcggc caggccgagt acctgcgggt cccgcacgcg cactacgggc cgatcaaggt
      541 gccggagggt ccgccggacg tgcggttcct cttcctctcc gacgtgctcc cgaccgcgtg
      601 gcaggccgtc gagtacgccg acgtcccgga cgacgggacc gtcgtcgtgt gggggctcgg
      661 tccgatcggt cagttgtcgg cgcgcgtcgc tcgccggcgg ggtgcgggcc gggtgatcgg
      721 cgtcgactcg gtcgccgaac gtcgcgcgat ggcggagcgg tggggcgtcg agacgatcga
      781 tgcccaggac ggcgacccgg tcgaggccgt ccgtgagcgc accgacggcc gcggcgccga
      841 cgccgggatc gacgcggtcg ggatggaggc cgaggggtcg ctcctcgagc cggtgctcca
      901 gggcgcgaag gtcgtgcccg accgcctgca cgcgctcaag cagacgatgg gggccgtccg
      961 ccgcggcgcc acggtctcgg tgtcgggcgt ctacacggcg tggttcccga agttcccgct
     1021 gggccagctc ttcgaccgtc agatctcgat ccggatgggc caggcgaacg tccggcggtg
     1081 ggtcgacgag atcatgccgc tgctcggcga cgacgacccg ctcggcaccg atgacctcgc
     1141 gacgcacacg gtcccgctcg gcgaggcccc caagatgtac gagcggttcc agaagaagcg
     1201 cgacggcacg atcaaggtcg tgctgcggcc ctagccgacg gcgttacggg cctacggcca
     1261 cgacggctcg tcggccggca tgatgagcag ctctcgcacg tcgagacccc gaggctgctg
     1321 cagcgcgaac acgacggcgt cggcgacgtc gctggggctc gcgaggttct ggtcgggcgc
     1381 cggccgatac ttctcggggc gttcgtcgaa gaacgcggtc gccatcccgc cggggatgag
     1441 gcacgtgacg ccgacccgtc ctcccatttc cgcggcgagc gcgcgcgtga acccgatcac
     1501 gccgaacttg ctggcggaat acgcggtcgc gtcggggagc gcgcggagcc cgagcgtcga
     1561 cgcgaccgtg acgatgcgcc cacggctccg ctcgagcgcc ggcaacgcgg ctcggacgac
     1621 cgccgccgtg ccgaggaggt tgacgcgtac cacgcgctcc cagtcgtcgc gggggacgtc
     1681 cgcgagcggc ccgcaggaat ccgtcccggc acaggtcacg accgcggaga ggtcgccgcg
     1741 ctccgcgacg cggcgcacgg cctcctcggt gcgctcggtg tcggagaggt cgacgatctc
     1801 gtggtccgcg ccggacggcg gctccatgcg gtcgaagacg accgcggccg ctcccgtctt
     1861 ctggatcgcg tccgcgaccg cagcccccag cccggaggca cctcccgtca cgatgatcgt
     1921 tccttcgatc gtcacgacgg ccttccgtgc ttcgtggtcc gcgcgaccgc ggccgccgac
     1981 cgctccacga cgctcgtgta gacctggagc gtcaggcgcg cgatgcggtc ccatccgtac
     2041 tgggccgccg cacgcgccgc tcctcgccgt cccatctcgg cgcggatctc ggggtcggcg
     2101 aggagtcggc ggatggcctc ggcgatcgag tcgctggcgc gcgggcggac gtggagcccc
     2161 ggcacgccct cgacgacggt gtcgacgaga ccgccgaccg cggacgcgac gacgggaacc
     2221 ccgcatgcca tcgcctcgac ggcgaccatt ccgaacggct cgtaccacgg ggtgcacgcg
     2281 acgaggtcgg cggatcgcaa cagcgcgggc acctgatcgc gctccacgcg tccgagcaga
     2341 cggacgcgat cggcgacgcc ggcctcctcg gcgaggcgca ggaaccgccg ggcctcggcg
     2401 tcgcgcgcga ggtcgccgct ctccgggccg cccgcgacga ggagctccgc gcccggaacg
     2461 cgccccatcg cggtgacgat gttgccgatc cccttccgct cgacgaggcg actgacgacg
     2521 acgacgcggt ccaggcccgg tgtcttgcgt tccgtgggcc catcgggccg gaagagcgaa
     2581 aggtcgacgc cacaggggat caccgacgtg cgtccggggt ctgtgccgag gcgcgcgagc
     2641 tcgaatacct cgtccgtgga cgtcgccacg atgtggtcga cggcctgcgt gagagcctcc
     2701 tcgatcgcga tgcgctcggg tggactcgtg tccttcgcgc cctggtgtcg ccgcttcacg
     2761 acgccgagtg cgtggaacgt gagcacgatc gggacgcccg tgcgtcggcc cgccgcgacg
     2821 gaagccttcg ccgacatcca gaaatgcgcg tgcgcgacgt cgggacgcca tgtgcgccac
     2881 gcgacctcga gctccgccgc gaaggcgtcc atgtggggca gtagctcgtc cttcgggagc
     2941 ggcttcggtg gccccgcgtc gacgtgctcg acggtgacgc cgggcgcgaa cttcacccgc
     3001 ttgggcaacg tggggtcgtc gcgtcgcgtg tacacgacga cg
//
LOCUS       DASZOB010000543         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_19204, whole genome shotgun sequence.
ACCESSION   DASZOB010000543 DASZOB010000000
VERSION     DASZOB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19204"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VG709_08545"
     CDS             complement(<1..183)
                     /locus_tag="VG709_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor"
                     /protein_id="HWC33160.1"
                     /translation="MNVTVRMFGALAEWAGRDRGELQLPDGATAGDALASVLAVTSQA
                     APVAGRVSVAVNLEVVP"
     gene            complement(180..1415)
                     /locus_tag="VG709_08550"
     CDS             complement(180..1415)
                     /locus_tag="VG709_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HWC33161.1"
                     /translation="MGDLDAPRPRAAVITVSDRAATGERDDRSGPAVTQALERAGFDV
                     VRRAVVADAVEEVRSAIAEAARSADLVATTGGTGLSPRDVTPEATRAVMEREAPGIAE
                     AMRAAGAASTPTAWLSRGVAGVVNGALVVNLPGSPSGAAESVEAIADILPHAVEVLRG
                     GSVHPEDRPSSSPGNARVVATAVRVVEGAPPCRPGQKLVLGAAGPIEGTLGCAEFDAA
                     AERAAAGVMATAEPSMRTYRHDLGSVEVLLERAVAPSPLFVLGATPVAAARLRGARDL
                     GYRPVLVEPRRERVMAAHRTAAVAVLDSLDAADVGPDADVVHTDHEASGLADEIEIAL
                     RRGARFVGVMGSARHVGRHLDELRARGLAGSELDRLQTPVGLDIGAVSPEEIALSILA
                     GLVAARNGRSGGWLAGRPA"
     gene            1460..>1816
                     /locus_tag="VG709_08555"
     CDS             1460..>1816
                     /locus_tag="VG709_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWC33162.1"
                     /translation="MAPLVDGFGRVVRDLRVSLIDRCNLRCTYCMPAEGLPWLAADDL
                     LSRAEIERLVRLFVRLGVRELQLTGGEPTVRPDVVEIVAALRAIDAGIEISMTTNGYL
                     LERLAEPLRAAGLDRVG"
BASE COUNT          214 a          714 c          679 g          209 t
ORIGIN      
        1 cggcaccacc tcgaggttca cggcgacaga gacccggccg gcgacgggtg cggcttggga
       61 cgtgaccgcg agcaccgaag cgagcgcgtc gcccgcggtc gcgccgtccg gcagctgcag
      121 ctcaccgcgg tcgcggccgg cccactcggc gagcgccccg aacatccgga ccgtgacgtt
      181 cacgcgggcc tcccggcgag ccagccgccc gagcggccgt tcctcgccgc gacgagcccc
      241 gccaggatcg acagcgcgat ctcctccggg ctcacggcgc cgatgtcgag cccgaccggc
      301 gtctgcaggc ggtccagctc cgagccggcg agcccgcgcg cacggagctc gtccaggtgg
      361 cgcccgacgt gccgggcgct gcccatcacg cccacgaacc gcgcgccgcg ccgcagcgcg
      421 atctcgatct cgtcggcgag cccggacgcc tcgtggtcgg tgtggacgac gtccgcatcg
      481 ggcccgacgt ccgcggcatc gagcgagtcg aggaccgcga cggcggcggt ccggtgcgcg
      541 gccatcacgc gctcccgacg gggttcgacc aggaccggcc ggtacccgag gtcgcgcgcc
      601 ccgcgcagcc gcgcggccgc caccggcgtc gcgccgagga cgaacaacgg cgacggcgca
      661 acggcccgtt cgaggaggac ctccacggag ccgaggtcgt gccggtacgt gcgcatggag
      721 ggctcggcgg tcgccatgac gcccgcggcg gcgcgctcgg cggccgcgtc gaactccgcg
      781 catccgagcg tcccctcgat ggggccggcc gcgcccagga cgagcttctg cccgggccgg
      841 cacgggggcg cgccctcgac cacgcggacg gcggtcgcca cgacgcgcgc gttgccgggc
      901 gacgaggacg gacggtcctc ggggtggacc gaccctccgc ggaggacctc gaccgcgtgc
      961 ggcaggatgt cggcgatcgc ctcgacgctc tcggctgcgc cggacgggct gcccgggagg
     1021 ttcacgacga gcgcgccgtt cacgaccccg gcgacgcctc gcgacagcca ggccgtcggc
     1081 gtcgacgcgg cgcccgccgc ccgcatcgcc tccgcgatcc ccggcgcctc gcgctccatc
     1141 accgcacgcg tcgcctccgg cgtcacgtcg cgcggcgaca gtcccgtgcc tcccgtggtg
     1201 gccaccaggt cggcggagcg ggccgcctcg gcgatcgcgc ttcgcacctc ctcgaccgcg
     1261 tcggcgacga ccgcacgtcg gacgacgtcg aacccggcgc gctcgagcgc ctgggtcacc
     1321 gccgggcccg agcgatcgtc gcgctcgccg gtggccgcgc ggtcgctgac ggtgatgacg
     1381 gcggcgcgcg gccggggtgc gtcgaggtcg cccatcgaac ccccgcatga tagggccgcc
     1441 gggtggctag gatggctccg tggccccgct cgtcgacggg ttcggacgtg tcgtccgcga
     1501 cctccgcgtc tcgctgatcg accgctgcaa cctccgctgc acgtactgca tgccggcgga
     1561 ggggctgcca tggctggcgg cggacgacct gctgtcccgc gccgagatcg agcggctcgt
     1621 gcggctgttc gtgcggctcg gcgtccgcga gctgcagctg accggcggcg agccgacggt
     1681 gcgcccggac gtcgtcgaga tcgtggcggc gctccgggcg atcgacgccg gcatcgagat
     1741 ctcgatgacg acgaacggct acctcctcga gcggctggcc gagccgctcc gggcggccgg
     1801 cctcgaccgc gtcggc
//
LOCUS       DASZOB010000544         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_181503, whole genome shotgun sequence.
ACCESSION   DASZOB010000544 DASZOB010000000
VERSION     DASZOB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181503"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1122
                     /gene="miaB"
                     /locus_tag="VG709_08560"
                     /pseudo
     CDS             1..1122
                     /gene="miaB"
                     /locus_tag="VG709_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            1137..2099
                     /gene="miaA"
                     /locus_tag="VG709_08565"
     CDS             1137..2099
                     /gene="miaA"
                     /locus_tag="VG709_08565"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWC33163.1"
                     /translation="MTTTVAPRRAVAEAAAVSVARALVGPTATGKTEASIELARRLGS
                     EIVLIDSTTVYRGMDVGTAKPSPEQRAAVVHHLVDVADPLEAFSVAEFQRLAREALRD
                     IASRGRAAILVGGSGLYYRAIVDDLDFPGRDARVRSSLEAEAAALGTEALYARLVHLD
                     PAAAAKIERGNVRRIVRALEVPAITGRPFSSYADAWDRYPSDHVRAAGVAMRPDTLRR
                     RIEARTRAQLDGALVEEVRALLDGGFRPFLTSSQAIGYLEVAEHLAGRLSLEEAYERI
                     VRRTRNLARRQIAWFRRDPRITWFETDEDGAAAIVDELEEWFSR"
     gene            2096..2779
                     /gene="dapF"
                     /locus_tag="VG709_08570"
     CDS             2096..2779
                     /gene="dapF"
                     /locus_tag="VG709_08570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWC33164.1"
                     /translation="MRFSKYHGTGNDFVLFEDLTHGLELGEQLVADVCDRHRGVGAAG
                     VIRIAPSERAAFFMDYRNADGQLAEMCGNGIRCLAKYVYDRGLTAKTELDVDTRAGVK
                     HLVLVGANLARLDHPRVGPPLEHHADFPERTNVEFIDVDDGVVNVRVWERGSGETMAC
                     GTGACASLVAANLMGLAPRRAIVRFPGGDIDVEWTEGTVLMTGPAEHVFDGTLADAWV
                     AARMETAQP"
BASE COUNT          437 a         1051 c         1051 g          432 t
ORIGIN      
        1 gccaaggagc gcagtccggc gatgcggatc gtggtcagcg ggtgcctggc ccagaaggac
       61 cgcggcgaga tccagcgccg ggccccgtgg gtcgacgtgg tgatcggcac ccacgccctg
      121 ccccacctga tcgacctgct ccggcgggcc gagaccgacg ggccgcagat ggacgtccgt
      181 gagtacaccg agacgttccc gagcgccctc ccggcccgcc gcgagttccg gcatgcggcg
      241 tgggtatccg tgtcggtcgg gtgcgacaac agctgctcgt tctgcatcgt cccattggtg
      301 cgcggccgcc agcgctcgcg accgctcggc gagatcctcg ccgaggtcca ggggctcgcc
      361 gccgaggggg tggtcgaggt gacgctgctc gggcagaacg tcaacacgta cggccgcgac
      421 gtctacttcc gcgactcgtc gccctcacgg cgcccgctgt tcgccgagct tctccgggcg
      481 gtgagtgacg tcgacgggat ccggcgcgtt cgcttcacca gccctcatcc gcacgacttc
      541 acgcccgacg tcatcgacgc gatggccgag agcgacgcgg tctgcgagca catccacttc
      601 ccgctccagt ccggctccga ccgcgtgctg cggctgatgc gccggtcgta ccgctcggcg
      661 cgctacctcg agtggctcgg gcggatccgc gaggcgatcc cggagatcgc cgtcagcacc
      721 gacatcatcg tcgggttccc cggcgagacc gaggaggact tcggggccac gctgcgagtc
      781 acggaggagg cccggttcga ctcggcctac acgttccagt actcgcctcg cgccggcacg
      841 gacgccgcgt cctacgagca gcaggtggcg aaggacgtcg tccaggagcg gttcgatcgt
      901 ctcgtggcgc tgcaggagag catctcgctc gagaagaacc gcacgctcga ggggacggtg
      961 gcggaggtcc tcgtcgaggg tcgcgggcgc aaggggggcg tgcaggcgcg aacccggacg
     1021 aacaaggtcg tgcacttcga cggcgatacg gacgcgggca ggttgctcga cgtgcgcatc
     1081 acgcgcgcgc atccgcatca cctctcgggt gaacccgtcg cggcgatggt gccggcatga
     1141 cgacgaccgt cgcacctcgt cgcgccgtcg ccgaagcggc cgcggtgtcg gtcgctcgcg
     1201 cgctcgtcgg tccgaccgcg accggcaaga ccgaggcgtc gatcgagctg gctcgtcggc
     1261 tgggaagcga gatcgtcctc atcgactcca cgaccgtgta ccgcggcatg gatgtcggga
     1321 ccgcgaagcc gtcgccggag cagcgcgcgg ccgtcgtgca ccacctcgtc gacgtcgcgg
     1381 atcccctcga ggcgttctcg gtcgcggagt tccagcggct ggcgcgcgag gcgctccgcg
     1441 acatcgcatc gcgcggacgc gcggcgatcc tcgtcggcgg ctcgggcctc tactaccgcg
     1501 cgatcgtgga cgatctcgac tttccgggca gggatgctcg cgtgcgctcc tcgctggagg
     1561 ccgaggcggc cgcgctcggc accgaggcgc tgtacgcccg gctcgtccac ctcgaccccg
     1621 ccgccgccgc caagatcgag cgcggcaacg tgcggaggat cgtccgcgct ctcgaggtcc
     1681 ccgccatcac gggtcgtccg ttctcctcgt acgcggacgc gtgggaccgg tatcccagcg
     1741 atcacgtccg agcggctggc gtcgcgatgc ggcccgatac gctccggcgc cgcatcgagg
     1801 cgcgaacacg cgcacagctc gacggagccc tggtcgagga ggtgcgcgcc ctccttgacg
     1861 ggggattccg accgttcctg acatcgtcgc aggcgatcgg gtacctcgag gtcgccgagc
     1921 atctcgcggg acggctctcg ctcgaggagg cgtacgagcg gatcgtccga cgcacgcgga
     1981 acctcgcgcg gcgccagatc gcgtggttcc gcagagaccc gcggatcacg tggttcgaga
     2041 ccgacgagga cggcgccgcg gctatcgtcg acgagctcga agagtggttc tcgcgatgag
     2101 gttctccaag taccacggga ccggcaacga cttcgtgctg ttcgaggacc tgacccacgg
     2161 cctcgaactc ggagagcagc tcgtcgccga cgtgtgcgat cgccaccggg gcgtgggggc
     2221 cgccggggtc atccgcatcg cgccgtcgga acgagccgcc ttcttcatgg actaccgcaa
     2281 cgcggacggc cagctcgccg agatgtgcgg gaacggcatc cggtgcctcg ccaagtacgt
     2341 ctacgaccgg gggctcaccg cgaagaccga gctcgacgtc gatactcgtg ccggcgtcaa
     2401 gcacctcgtg ctcgtcggtg cgaacctcgc acggctcgac cacccgcggg tcgggcctcc
     2461 gctcgagcac catgccgact ttcccgaacg caccaacgtc gagttcatcg acgtcgacga
     2521 cggcgtcgtc aacgtacgcg tgtgggagcg cggctccggc gagacgatgg cgtgcgggac
     2581 gggcgcgtgc gcgtcgctgg tcgctgccaa cctgatgggg ctcgccccgc gccgcgccat
     2641 cgtgcggttc cccggcggtg acatcgacgt cgagtggacg gaggggacgg tgctcatgac
     2701 cggcccggcc gagcacgtgt tcgacggcac gctcgcggac gcgtgggtcg ccgcgaggat
     2761 ggagaccgcg caaccgtgag ggccgcacgt cgggtgcggt ctctcccgcc ctacatgttc
     2821 gcggagctcg accgcaagat agccgagagc aaggccgccg gcgacaacgt gatctcgctc
     2881 ggcgtcggcg accccgacct cccgacgccg cgtcacgtcg tcgaggcgct ccaggaagcc
     2941 gcggaggacc caacgacgca tggatacccg t
//
LOCUS       DASZOB010000545         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_49975, whole genome shotgun sequence.
ACCESSION   DASZOB010000545 DASZOB010000000
VERSION     DASZOB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49975"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VG709_08575"
     CDS             complement(<1..1248)
                     /locus_tag="VG709_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC33165.1"
                     /translation="MRDHPQIRTFARLLGFLRPYRWSLAVSVVLAVGSQAAAVTIAFL
                     TGDALESAVRSRDPGQLWFLAVLVLAVGLARALFMVGRRLISGRQALGVEYDLRDALY
                     AKLLRLSFGFYDRHQTGQLMSRATVDLQAVRFFLGYGLIFFFQHILTIVGVTVVLFVV
                     NWRLALAATAIMPFLVAVAYRYSRVSHPILRDVQQKMADVATVAEENIVGVHVVKSFA
                     RERDESAKFADRSERVFGQSVRANRQRATYVPLLAFLPLAAQAAVLLLGGRMVARGEL
                     DLSAFFAFNVLVVMLVFPLRMLGMWIGQAQRATASGERIFEVVDEDEDVTDRLGARDL
                     PPGEGHIRFERATFAYAPGRPVLEDIDLDVAAGQTVALIGHTGAGKTTLASLVPRFYD
                     VADGRLTIDGTDVRDVTLRSLRRA"
     gene            1419..>1651
                     /locus_tag="VG709_08580"
     CDS             1419..>1651
                     /locus_tag="VG709_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HWC33166.1"
                     /translation="MPLPLESVPNFSEGRDGDVLAELREAMSGPARLLDVHADWDHHR
                     SVFTLVGDGDELAQALLAGIRRAVERIDLRRHEG"
BASE COUNT          266 a          570 c          611 g          204 t
ORIGIN      
        1 cgcgcgccgg agcgatcgca gggtgacgtc acggacgtcg gtaccgtcga tcgtcaaccg
       61 tccgtccgcc acgtcgtaga agcgcgggac gagcgaggcg agcgtcgtct tccctgcgcc
      121 ggtgtgcccg atgagagcga cggtctggcc cgcggcgacg tcgaggtcga tgtcctcgag
      181 caccgggcgg ccgggtgcgt acgcgaacgt cgcgcgctcg aagcggatgt gcccctcccc
      241 cggcggaaga tcgcgcgcac ccaggcgatc ggtgacgtcc tcgtcctcgt cgaccacctc
      301 gaagatccgc tcgcccgacg cagtcgcgcg ctgcgcctgg ccgatccaca tcccgagcat
      361 gcgcagcggg aagaccagca tgacgacgag cacgttgaag gcgaagaagg cggacaggtc
      421 gagctcgccg cgcgcgacca tccgcccgcc gagcagcagg acggccgcct gcgcagcgag
      481 cggcaggaag gcgaggagcg gcacgtacgt cgcgcgctgg cggttcgccc gcacgctctg
      541 cccgaacacc ctctcggagc ggtcggcgaa cttggccgac tcgtcccgct cgcgcgcgaa
      601 cgacttgacg acgtggacgc cgacgatgtt ctcctccgcg actgtcgcga cgtcggccat
      661 cttctgctgg acgtcgcgca ggatcgggtg cgaaacgcgg ctgtagcggt aggcgaccgc
      721 gacgaggaac ggcatgatcg ccgtcgcggc gagcgcgagc cgccagttga cgacgaagag
      781 cacgaccgtc acgccgacga tcgtgaggat gtgctggaag aagaagatga ggccgtagcc
      841 gaggaagaag cggacggcct gcaggtcgac ggtggcgcgg gacatcagct ggcccgtctg
      901 atggcggtcg tagaagccga acgagaggcg caggagcttc gcgtagagcg cgtcgcgcag
      961 gtcgtactcg acgccgagcg cctggcgccc cgagatgagg cggcgcccga ccatgaagag
     1021 cgcgcgggcg agccccacgg cgaggaccag gaccgcaagg aaccagagct ggccggggtc
     1081 gcgggagcgg acggcgctct cgagcgcatc ccccgtgagg aaggcgatcg tcaccgcggc
     1141 tgcttgcgat ccgaccgcga gcacgaccga gacggcgagc gaccagcggt acgggcgcag
     1201 gaagccgagg aggcgcgcaa acgtccgaat ctgtgggtga tcgcgcatta gtgggagcta
     1261 acgtcgagtg taccggcggc ggtccgcgca tccccggcgc cggccggatc ttcgttcacg
     1321 agtcgtcgca ctccgcgcgc cacgcgcgcg ttcgcgcacg tgatgcgcgc cgtggaggga
     1381 ggcccgcggc ggccccgggg cgctcgtatg cttcgccggt gccgctgccg ctcgagtcgg
     1441 tccccaactt ctccgagggc cgcgacggcg acgtgctggc cgagctgcgc gaggcgatgt
     1501 ccggcccggc gcgcctcctc gacgtccacg ccgactggga ccaccaccgc tcggtgttca
     1561 cgctcgtcgg ggacggcgac gagctcgcgc aggcgctcct cgcgggcatc cgccgcgccg
     1621 tcgagcgcat cgacctccgc cgccacgagg g
//
LOCUS       DASZOB010000546         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_19367, whole genome shotgun sequence.
ACCESSION   DASZOB010000546 DASZOB010000000
VERSION     DASZOB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19367"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1325
                     /locus_tag="VG709_08585"
     CDS             174..1325
                     /locus_tag="VG709_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWC33167.1"
                     /translation="MAGAGPENGQADVRLERVTKRFAEVVAVDAIDLEVARGQFFSLL
                     GPSGCGKTTTLRMIGGFEHPDDGRIELAGRDVSNLPPHRRDVNTVFQSYALFPHLNVF
                     ENVAYGLRRKRVDKAEIRRRVVDMLELVDLPGFERRRPSQLSGGQQQRVALARALVNR
                     PQVLLLDEPLGALDLKLRKQMQLELKRIQHDVGITFIYVTHDQEEAMTMSERIAVMRD
                     GRVEQVGAPQETYEHPATAFVAGFLGASNLLGATVGPQERDTRIVALDSGEKVRVPLS
                     ALDGLGGRIQVGVRPEKIHVRPSGGDPTAGENAIDGRVIDATFVGVSNVYTVETRAGQ
                     RVTVYVQNLAGASAWTPGPGENVTLSWDPEHTFVVGAEDRAGGGDGEEA"
     gene            1312..>2345
                     /locus_tag="VG709_08590"
     CDS             1312..>2345
                     /locus_tag="VG709_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWC33168.1"
                     /translation="MRRRDRLPTGWGRPLSRRELLRQAGRGSLGLAAMSFLAACGVRS
                     ERPEQPAAQSPSPLPPKAGELQIANWPLYIDKAKGEPVTPQTFSEEHDVDVTYREVIN
                     DNVEFFATIQEPLAQGEAAGWDIIVVTDWLVDKMNQLGYLEQLHHSELPQVQENLRDT
                     FRDPWYDAGNAHSVPWAAGFTGIGYDISLTGRELTSIDDLFDDEWAGRVGLFTEMRDT
                     FGLVLLSLGIEPSEATVEDAERAQQKLLENRDNVRGFYGNEYADQLATGSLAVSMAWS
                     GDIFALQLDNPNLRFALPEQGAMRWVDNMVIANGAEHPTDAHLFMDYVYDPRIAANIT
                     EWVWYESPVEP"
BASE COUNT          399 a          773 c          835 g          338 t
ORIGIN      
        1 gcatcatatc ccgcgcccgc tcgtccgagc gaggcgcgaa accgccggtc agagggcgtc
       61 cggcgcttgt gaaaactttc acaagcacca ctatacagag cggcgaagcg gcgaggcaac
      121 cgtaggccgc gagcggaatg cgtgacatga gggccacggc tacgctgtcc gcgatggcgg
      181 gtgcggggcc ggagaacggg caagcagacg tccggctcga gcgcgtcacg aaacggttcg
      241 cggaggtcgt cgcggtcgac gccatcgacc tcgaggtcgc gcgcgggcag ttcttcagcc
      301 tcctcggccc ctccggttgc gggaagacca cgacgctccg gatgatcggc gggttcgagc
      361 accccgacga cggacgcatc gagctcgccg gacgcgacgt ctcgaacctc ccgcctcacc
      421 ggcgcgacgt caacaccgtt ttccagtcgt acgccctgtt cccccacctg aacgtcttcg
      481 agaacgtcgc gtacgggctg aggaggaagc gggtcgacaa ggcggagatc cgccgtcgcg
      541 tcgtcgacat gctcgagctc gtcgacctcc ctgggttcga acgccgacgg ccgtcgcagc
      601 tctcgggtgg ccaacagcag cgcgtcgcgc tcgcgcgggc gctcgtgaac cggcctcagg
      661 tcctgcttct cgacgagccg ctcggcgccc tcgacctcaa gctccgcaag cagatgcagc
      721 tcgagctcaa gcggatccag cacgacgtcg gcatcacgtt catctacgtg acccacgatc
      781 aggaggaggc gatgacgatg tccgagcgca tcgctgtgat gcgcgacgga cgcgtcgagc
      841 aggtgggcgc gccgcaggag acgtacgagc atcccgcgac cgcgttcgtc gcgggcttcc
      901 tcggagcgtc gaacctcttg ggtgccacgg tcgggccgca ggaacgcgac acgcggatcg
      961 tcgcgctcga ctccggcgag aaggttcgtg tgccgctctc cgcgctcgac gggctcggcg
     1021 ggcggatcca ggtcggcgtc cgcccggaga agatccacgt aaggccctcg ggcggcgatc
     1081 cgaccgccgg agagaacgcg atcgacgggc gcgtgatcga cgcgacgttc gtcggcgtca
     1141 gcaacgtcta cacggtggag acgcgcgcag gacaacgcgt gacggtgtac gtccagaacc
     1201 tcgcgggtgc gagcgcgtgg acgcccggcc ccggcgaaaa cgtgacgctg tcgtgggacc
     1261 cggagcacac gttcgtcgtc ggggcggagg accgagcggg aggaggcgat ggtgaggagg
     1321 cgtgatcgtt tgccgacggg atggggacgc ccgctgtctc gacgtgagct gctgcgtcag
     1381 gcgggtcgag ggtcgctcgg gctcgccgcg atgtcgttcc tcgcggcgtg cggcgttcgg
     1441 agcgagcggc cggagcagcc tgccgcgcag tcgccgtcgc cgctcccgcc gaaggccggg
     1501 gagctgcaga tcgcgaactg gccgctctac atcgacaagg cgaagggcga gccggtcacg
     1561 ccgcagacct tctcggagga gcacgacgtc gacgtgacct accgtgaggt catcaacgac
     1621 aacgtcgagt tcttcgcgac gatccaggag ccgctcgcac agggcgaggc ggccgggtgg
     1681 gacatcatcg tggtcaccga ctggctcgtc gacaagatga accagctcgg gtacctcgag
     1741 cagcttcacc actcggagct cccgcaggtg caggagaacc tccgggacac cttccgcgac
     1801 ccgtggtacg acgcggggaa cgcgcacagc gtcccgtggg cggcgggctt caccggcatc
     1861 ggctacgaca tctcgcttac cgggcgcgag ctgacgagca tcgacgacct cttcgacgac
     1921 gagtgggccg ggcgcgtcgg gctgttcacg gagatgcggg acacgttcgg gctcgtgctg
     1981 ctctcgctcg ggattgagcc gtcggaggcg acggtcgagg acgccgagcg cgcgcagcag
     2041 aagctgctcg agaaccgcga caacgtccgt ggcttctacg gcaacgagta cgcggaccag
     2101 ctggcgacgg gaagcctggc ggtgtcgatg gcgtggtcgg gcgacatctt cgctctccag
     2161 ctcgacaacc cgaacctgcg gttcgcgctg ccggagcagg gcgcgatgcg ctgggtcgac
     2221 aacatggtga tcgcgaacgg agccgagcac ccgaccgacg cgcacctgtt catggactac
     2281 gtctacgacc cgcggattgc ggcgaacatc acggagtggg tctggtacga gtcccccgtc
     2341 gagcc
//
LOCUS       DASZOB010000547         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_50010, whole genome shotgun sequence.
ACCESSION   DASZOB010000547 DASZOB010000000
VERSION     DASZOB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50010"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..225
                     /locus_tag="VG709_08595"
     CDS             16..225
                     /locus_tag="VG709_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33169.1"
                     /translation="MANIKLGKPDASPDDPAHTPGVKEGNSTGNYEKSPGHLADGRST
                     AERSTGVNPGKHGPIDPRMPNLSPP"
     gene            227..868
                     /locus_tag="VG709_08600"
     CDS             227..868
                     /locus_tag="VG709_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HWC33170.1"
                     /translation="MAAVPPELEFAVEEVGAAPFAAVPTLLFALRVDANGADVRALSL
                     NVQVRIDAARRSYREGDDARLLEIFGRPEAWATTLRSLLWTQASLAVPGFAGETVVEL
                     PVACTYDFDVVAAKYLHALDAGDVPLELLFSGTAFYPGDDGRLQAAPVPWSSEAKARL
                     PVRVWREAVDHAFPGTAWLRVARDTFERLSEFRNARTLPRWDAALAELLEGRS"
     gene            865..1806
                     /locus_tag="VG709_08605"
     CDS             865..1806
                     /locus_tag="VG709_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33171.1"
                     /translation="MSDAVRRIADAVLYEGHTLWPYRRSALKNRQRWTFGGVYPETYA
                     RASGDRSAVQAECLLEGEEPVVEVEVRFLQAVRRQAVRDGEPVDELDGHLSWDETTER
                     VVRVPPGARATAIAIPAGTDVEAVAGGELVRSWEPLTGRVTVDVEPLARRVHRVRVRV
                     VNDSTGRAEAREEAVPRTFLSAHAVVRALAGELLSAPDPPERVREHAASLRTEGLWPV
                     LVGDEDARDTILAAPIILSDYPQVAPESPGDFFDGCEIDQLLVLNILGLTDDEKREMR
                     ASDPRAREILERTEAMTPEEMLRLHGVIRDLRPVEGR"
     gene            1803..>2125
                     /locus_tag="VG709_08610"
     CDS             1803..>2125
                     /locus_tag="VG709_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33172.1"
                     /translation="MSFDVWRELERPGPESVVVQGIELRSGARVRLWPSVRRDVWDTA
                     LKGRPAVVETIEELVDGGVVLTVSPEDDPGRDLGAARPGHRFFFTPDEVEPLTRAPIL
                     VAGIG"
BASE COUNT          279 a          789 c          775 g          282 t
ORIGIN      
        1 gtaaggagga gtgcgatggc gaacatcaag ctcggcaagc cggacgcgag ccccgacgat
       61 cccgcccaca cgccgggtgt caaggagggg aactcgaccg gaaactacga gaagtcgcca
      121 gggcacctgg cggacggccg ctcgacggcc gagcgctcca ccggcgtcaa cccgggcaag
      181 cacgggccga tcgacccgcg gatgccgaac ctgtcgcctc cgtagcgtgg ccgcggttcc
      241 gcctgagctc gagttcgccg tcgaggaggt cggggccgcg ccgttcgctg ccgtgccgac
      301 gctgctcttc gcgctccgcg tggacgcgaa cggggcggac gtgcgcgcgc tctcgctgaa
      361 cgtgcaggtc cggatcgacg ccgcccggcg ttcgtaccgc gagggcgacg acgcccgcct
      421 gctcgagatc ttcggccgcc cggaggcgtg ggcgactacg ctccgcagcc tcctctggac
      481 gcaggcctcg ctcgccgtcc ccggcttcgc cggcgagacc gtcgtcgagc tgccggtcgc
      541 gtgcacgtac gacttcgacg tcgtcgccgc caagtacctc cacgcgctcg acgccggaga
      601 cgtcccgctc gagcttctct tcagcgggac ggcgttctac ccgggcgacg acggccggct
      661 ccaggcggcg cccgtcccgt ggagctccga ggcgaaggcg cgcctgcccg tgcgcgtctg
      721 gcgcgaggcg gtcgaccatg cgttccccgg cacggcgtgg ctccgagtcg cccgcgacac
      781 gttcgagcgg ctgtcggagt tccgaaacgc gcgcacgctc ccgcgctggg acgccgcgct
      841 cgccgagctc ctcgaaggcc gctcgtgagc gacgccgtgc gtcggatcgc cgacgccgtc
      901 ctctacgagg gccataccct ctggccctac cggcgatcgg cgctcaagaa ccggcagcgc
      961 tggacgttcg gcggcgtcta cccggagacg tacgcgaggg cgagcggcga ccgctccgcc
     1021 gtccaggcgg agtgcctgct cgagggggag gagccggtgg tcgaggtcga ggtgcgcttc
     1081 ttgcaggcgg tccgccggca ggccgtccgg gacggcgagc ccgtggacga gctcgacggc
     1141 cacctgagct gggacgagac gaccgagcgc gtcgtccgcg ttccgcccgg cgcgcgcgcg
     1201 acggcgatcg cgatcccggc cgggacggac gtcgaggcgg tcgcgggcgg cgagctcgtg
     1261 cgctcctggg agccgctcac cggacgcgtc accgtcgacg tcgagccgct cgcgcgacgc
     1321 gtccaccgcg tgcgcgtgcg cgtcgtcaac gactctacgg ggcgcgcaga ggcgcgcgag
     1381 gaggcggttc cgcggacgtt cctctcggcg cacgccgtcg tccgcgcgct cgccggggag
     1441 ctcctctcgg cgcccgaccc gcccgagcgg gtccgcgagc acgcggcctc gctccggacc
     1501 gaggggctct ggcccgtgct cgtcggcgac gaggacgcgc gggacacgat ccttgccgcg
     1561 ccgatcatcc tctccgacta tccgcaggtc gcgcccgaga gccccggcga cttcttcgac
     1621 ggctgcgaga tcgaccagct gctcgtcctg aacatcctcg gcctcaccga cgacgagaag
     1681 cgtgagatgc gcgcgtccga cccacgcgcg cgcgagatcc tcgagcggac ggaggccatg
     1741 acgcccgagg agatgctccg cctccacggc gtcatccgcg accttcggcc cgtggaggga
     1801 cgatgagctt cgacgtctgg cgggagctcg agcggccagg gcccgaaagc gtggtcgtcc
     1861 aggggatcga gcttcgctcc ggcgcacgcg tgcggctctg gccgtccgtc cggcgcgacg
     1921 tctgggacac cgcgctcaag gggcgaccgg ccgtcgtcga gacgatcgag gagctcgtcg
     1981 acggcggcgt cgtcctcacc gtctcgcccg aggacgaccc ggggcgcgat ctcggcgccg
     2041 ctcgtccggg gcaccgcttc ttcttcaccc ccgacgaggt cgagccgctc acgcgggcgc
     2101 cgatcctggt cgcggggatc gggaa
//
LOCUS       DASZOB010000548         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_39928, whole genome shotgun sequence.
ACCESSION   DASZOB010000548 DASZOB010000000
VERSION     DASZOB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39928"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VG709_08615"
     CDS             complement(<1..196)
                     /locus_tag="VG709_08615"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWC33173.1"
                     /translation="MTRATIDEAASVVAGGGVVAFTGAGISAESGIPTFRDPGGRWDR
                     FDPSDFGTWDGLARTAMERPD"
     gene            complement(193..900)
                     /locus_tag="VG709_08620"
     CDS             complement(193..900)
                     /locus_tag="VG709_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWC33174.1"
                     /translation="MEERLRAPWSPWEAVPLALAAFTTAFVVAILLTAVLGGPGGPTA
                     VLAEIAFEGAMFAFTLAWVRIRYPDDAPALGLGSRRPGRDAFVGAVVGLALFFAAAIV
                     LLRGILFVFEALGIQPPTIQQIRFPLSPLNLALGLFLVVVAAPFAEEVFFRGFLYTSL
                     RGRLGVWPAALVSSAIFGVFHVSGGPILVPLLFLVGLAFAFLYEWRRSLVASIAAHAT
                     FNAIGYTLIIYERLFST"
     gene            complement(885..>1785)
                     /locus_tag="VG709_08625"
     CDS             complement(885..>1785)
                     /locus_tag="VG709_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33175.1"
                     /translation="GVALQVVMLPGSYLPHFDVSLVLGVLAAGAAALTVFALVVGRAS
                     PLLPVAAVGAAAVASVAVDAAFGWPSLLTPLLGGSALDGVRFYGLGNAYAGVLLSGVV
                     LVAAVVPASAGAALILASAVFAGAPWFGADFGGGLTLFAVAGVWIALRTRRRLDAAAV
                     GIVAASFVLGFAAIVGMHQVAEDPTHVTRATGGATGLAGVVETFWHRLRLNLESTAAT
                     PVVWPALLGLPAWLAVAWRRAGPFGRALEARDAWRDAVIVLAVGGILGYVLNDTFGMA
                     SIAFLYLSLAMVFPALAVRWKSG"
BASE COUNT          336 a          668 c          642 g          139 t
ORIGIN      
        1 cgtcgggacg ctccatcgcg gtccgcgcga gcccgtccca cgtcccgaag tccgacgggt
       61 cgaacctgtc ccagcgcccg ccggggtccc ggaacgtcgg gatcccggac tccgcggaga
      121 tcccggcgcc cgtgaacgcg acgacgccgc ccccggcgac gacagaagcc gcctcgtcga
      181 tcgtcgcgcg cgtcacgtcg agaacaaccg ctcgtagatg atcagcgtgt acccgatcgc
      241 gttgaacgtc gcgtgcgccg cgatcgacgc gaccagagac cgccgccact cgtacaggaa
      301 cgcgaacgcg agcccgacga gaaagagcag cggcacgagg atcggaccac cggagacgtg
      361 gaacacgccg aagatcgccg acgagacgag cgccgccggc cacacgccga gtcggccgcg
      421 cagcgacgtg tagaggaagc cgcggaagaa gacctcctcc gcgaacggag cggcgaccac
      481 gacgaggaac agcccgagcg cgaggttgag cggcgagagc gggaagcgga tctgctggat
      541 cgtgggcggc tggatgccca gcgcctcgaa cacgaacagg atcccgcgga ggagcacgat
      601 cgcggccgcg aagaacagcg cgagcccgac gaccgcgccg acgaacgcgt cgcgaccggg
      661 gcgccggctc ccgagcccga gggccggcgc atcgtccgga taccggatcc ggacccacgc
      721 gagcgtgaac gcgaacatcg cgccctcgaa cgcgatctcc gcgaggacag cggtggggcc
      781 tccgggtccc ccgagcacgg ccgtcagcag gatcgcgacc acgaaggcgg tcgtgaaggc
      841 cgcgagcgcg agcgggaccg cctcccacgg cgaccacggc gccctcagcc gctcttccac
      901 cggaccgcca gggcggggaa caccatcgcg agtgaaaggt agaggaaggc gatcgacgcc
      961 atcccgaacg tgtcgttgag cacgtacccg aggatgccgc cgaccgcaag cacgatcacc
     1021 gcgtcgcgcc acgcgtcgcg tgcctccagc gcccgcccga acggcccggc acggcgccag
     1081 gcgacggcga gccacgcggg gagcccgagg agcgccggcc agacgacggg cgtggccgcc
     1141 gtcgactcga ggttcagccg gagccggtgc cagaacgtct ccacgacgcc cgcgaggccg
     1201 gtggcgccac cggtcgcgcg cgtgacatgc gtcgggtctt cggcgacctg atgcatgccg
     1261 acgatggccg cgaacccaag cacgaacgac gccgcgacga tgccgacggc ggccgcgtcg
     1321 agccgccggc gcgtccggag cgcgatccag accccggcga ccgcgaacag cgtgagcccg
     1381 ccaccgaagt ccgcgccgaa ccacggcgcc cccgcgaaga ccgcggacgc caggatcaac
     1441 gccgcgccgg ccgaagccgg gaccaccgcc gcgacgagga cgacgccgga gagcagcacg
     1501 cccgcgtacg cgttcccgag cccgtagaag cgcacgccgt cgagggcgct ccctccgagg
     1561 agcggcgtga ggagcgacgg ccacccgaac gcggcgtcca cagcaaccga ggcgaccgcg
     1621 gccgcgccga ccgccgcgac cggcagcaag gggctcgccc gcccgacgac gagggcgaag
     1681 accgtgagcg ccgcggcgcc ggcggcgagg acgcccagga ccagcgagac gtcgaagtgc
     1741 gggagatagc tgcccggcag catcacgacc tggagggcca cgccg
//
LOCUS       DASZOB010000549         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_19809, whole genome shotgun sequence.
ACCESSION   DASZOB010000549 DASZOB010000000
VERSION     DASZOB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19809"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VG709_08630"
     CDS             complement(<1..322)
                     /locus_tag="VG709_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33176.1"
                     /translation="MSTTRLRRRLRAATIGLTVLASGLGLVPGPVLGRGAPPGVGRAV
                     PVAGEALGPATASATRAPSGGVRLDEPAWTAPRTTCAPIRFTMLGLVWRQRGPGEIPV
                     RVSWG"
     gene            430..>1554
                     /locus_tag="VG709_08635"
     CDS             430..>1554
                     /locus_tag="VG709_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWC33177.1"
                     /translation="MAEREPQRASSVQEFVRRVEAIEDAVREIASAMRQVAQRVAASS
                     DVVPMVAELAREVSSSLERLRSDVESVREQVGRQVPAAFDERVGRIPGEVDEALGTRL
                     RTSVDAISERTTGALDGLRAAVSGLEERLASLVSGGSEATQTRLGAAVDALTSQLRSL
                     EGRLESAVAAATSTTQERLAGALDALASGLQDVEERLGQTITGDALRPLEGLPQQLAD
                     AMAVVRDVLTEVAQAQGSVEQRLDEVREQGLRRTDELETGLADALTGVAETLREEQQR
                     ATDRLSERLDEIRGIVREEVEARVRDVEQAVRATIAERSEETRDAVAARVEEIRGAVA
                     DRLEEMPGAVGERADAAVERVAERIAARGDDVVAARVADIE"
BASE COUNT          213 a          541 c          622 g          178 t
ORIGIN      
        1 cgccccacga gacccggacg gggatctcgc cgggtccccg ctgccgccac acgagcccga
       61 gcatcgtgaa gcggatcggg gcgcaggtcg tccgtggcgc ggtccacgcg ggctcgtcga
      121 gccgcacgcc gcccgacggc gcgcgcgttg cgctcgcggt cgccgggccg agcgcctcgc
      181 cggcgaccgg caccgcgcgc ccgacgcccg gcggcgcgcc ccggccgagg accgggcccg
      241 gcacgagccc gaggcccgag gcgagcaccg tcaggccgat ggtggcggcc ctgaggcgac
      301 gacgaagacg ggttgtcgac atgggagacc cgccattctg ccgattcggt cgcgctacgt
      361 aaagcgtgtc cgtgcgtgaa acgcgaagtt cccttgattc gcgaaggtag aatccggcgc
      421 gagcgcctca tggcggagcg ggaaccccaa cgcgcatcgt ccgtccagga gttcgtccgc
      481 cgcgtcgagg cgatcgagga cgccgtacgg gagatcgcgt cggcgatgcg ccaggtcgcg
      541 cagcgcgtcg cggccagctc ggacgtcgtg ccgatggtcg ccgagctcgc gcgcgaggtg
      601 tcctcctcgc tggagcgact tcgttccgat gtcgagtcgg ttcgcgagca ggtcgggcgc
      661 caggtcccgg cggcgttcga cgagcgggtc ggacggatcc ccggcgaggt cgacgaggcg
      721 ctcggcacga ggctccggac ctcggtggat gcgatctcgg agcgaaccac gggcgcgctc
      781 gacggcctcc gcgcggccgt ctccggcctc gaggagcgcc tcgcgtcgct cgtctcgggc
      841 gggagcgagg cgactcagac gcgactcggc gccgccgtcg acgcgctgac gtcacagctc
      901 cgctcgctcg agggccggct cgagtcggcg gtcgccgccg cgacctcgac gacgcaggag
      961 cggctcgcgg gcgcgctcga cgcgctcgcc tccggactgc aggacgtcga ggagcggctc
     1021 gggcagacca tcaccggcga cgcgctgcgg cccctcgagg ggctgccgca gcagctcgcg
     1081 gacgcgatgg cggtcgtgcg cgacgtcctc accgaggtcg cccaggcaca gggttccgta
     1141 gagcagcgcc tcgatgaggt ccgcgagcag gggctgcggc ggaccgacga gctcgagacc
     1201 gggctcgccg acgcgctgac gggggtcgcc gagacgctcc gcgaggagca gcagcgggcg
     1261 accgaccgac tctccgagag gctcgacgag attcgcggga tcgtgcggga agaggtggag
     1321 gcacgcgtcc gcgacgtcga gcaggcggta cgggcgacga tcgcggagcg ctcggaggag
     1381 acgcgggatg cggtcgcggc gcgcgtggag gagatccgtg gcgcggtcgc cgaccgtctg
     1441 gaggagatgc ccggcgccgt cggcgagcgc gcggacgcgg cggtggagcg ggtcgccgag
     1501 cgcatcgcgg cacgcggcga cgacgtggtc gcggccaggg tcgcggacat cgag
//
LOCUS       DASZOB010000550         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U5933
            k141_40220, whole genome shotgun sequence.
ACCESSION   DASZOB010000550 DASZOB010000000
VERSION     DASZOB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956752
            Sequence Read Archive: SRR12102597
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 09:27:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,686
            CDSs (with protein)               :: 1,686
            Genes (RNA)                       :: 15
            tRNAs                             :: 12
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40220"
                     /isolate="SMAG_U5933"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="Israel"
                     /lat_lon="30.785336 N 34.767347 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VG709_08640"
     CDS             <1..185
                     /locus_tag="VG709_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWC33178.1"
                     /translation="LGLLAKEQAVYAFVFEGQRFDIGKKIDYLRATVELATSREDLGP
                     EFRAFLADLVQRKKLV"
     gene            224..1447
                     /gene="glp"
                     /locus_tag="VG709_08645"
     CDS             224..1447
                     /gene="glp"
                     /locus_tag="VG709_08645"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWC33179.1"
                     /translation="MPTEPLSLDDARKRILDAAPPIASIDLPLAEVQGCVLAREVVGE
                     YDLPPFSAAAADGFAVRAADIFGASEMKPVPLRIAGSVRSGRPPDATVGWGEAVRVVR
                     GAPIPAGADCVVPGGAFRLEGEQVEVVRQLSAGEHIDPAGEDVRAGSVLVPAGRRLSA
                     AEVGLLAAAGHATAVAYPKMRVAVVSVGPTLVEPGRPAAFGQLRDANSFSIVAALREA
                     GTVPYRLPLVRDADAELRELLLSNLSRADAFVCSGAMEDDADIPALLAGVGEIETYRV
                     QMYPGGAIGFGLVEGQPFFSLPAEPVSAFVAFEALVRPVILQMMGRRDLARPEVTAVL
                     DRPIGGPAGLTLFVPARVGRRDGRWHCVPTGPAHPRRLAAVVEANGLVVVPPGDRGLG
                     AGEEVRVQIFRPLDR"
BASE COUNT          197 a          596 c          573 g          255 t
ORIGIN      
        1 cgctcgggct cctcgcgaag gagcaggccg tctacgcgtt cgtgttcgag gggcagcgct
       61 tcgacatcgg caagaagatc gattacctcc gggcgaccgt cgagctcgcg acctcgcgag
      121 aggacctcgg ccccgagttc cgtgcgttcc tcgcggacct cgttcagcgc aagaagctcg
      181 tctgaggcgc ggaacgccgg ggctccggcg ataggatgcc gacatgccca ccgaaccgtt
      241 gtcgctcgac gacgcccgta agcggatcct cgacgcggcg ccgccgatcg cttcgatcga
      301 cctgccgctc gccgaggtgc agggctgcgt gctcgctcgc gaggtcgtcg gcgagtacga
      361 cctcccgccg ttctcggccg ccgccgccga cgggttcgcc gtccgcgccg ccgacatctt
      421 cggcgcgagc gagatgaagc cggtaccgct gcgcatcgcg ggatcggttc gctccgggcg
      481 gccccccgac gcgaccgtcg gatggggaga ggccgtccgc gtcgtacgcg gcgcgccgat
      541 ccccgccggc gcagactgcg tcgtccccgg cggcgcgttc cggctcgagg gagagcaggt
      601 ggaggtcgtc cgtcagctct ccgccggcga gcacatagat ccggcgggcg aggacgtccg
      661 tgcgggatcc gtcctcgtgc cggcggggcg tcggctctcg gccgcggagg tcgggctgct
      721 cgccgccgcg ggccatgcga ccgcagtcgc gtacccgaag atgcgcgtcg cggtcgtctc
      781 ggtcggcccg acgctcgtcg agcccggccg cccggcggcg ttcggccagc tgcgcgacgc
      841 gaactcgttc tcgatcgtcg ccgcgctgcg cgaggccggc acggtcccgt accgacttcc
      901 gctcgtccgt gacgccgatg cggaactgcg cgagctcctg ctttcgaacc tctcgcgtgc
      961 cgacgcgttc gtgtgctccg gcgcgatgga ggacgacgcc gatatcccgg cgctgctcgc
     1021 gggcgtcggg gagatcgaga cgtaccgggt ccagatgtac ccgggcggcg cgatcggctt
     1081 cggcctcgtc gagggccagc cgttcttctc gctgcccgcc gagccggtct cggcgttcgt
     1141 cgccttcgag gcgctggtac gcccggtgat cctgcagatg atggggcgcc gcgacctcgc
     1201 tcgccccgag gtcactgcgg ttctcgaccg cccgatcggc ggccccgcag ggctcaccct
     1261 cttcgtcccg gcccgcgtcg gtcgccgcga cggacgctgg cactgcgtcc cgaccggccc
     1321 cgcacacccg cggcggctcg ccgccgtcgt cgaggcgaac ggcctcgtcg tcgtcccgcc
     1381 cggcgaccga ggtctcggtg ccggcgagga ggtccgggtc cagatcttcc gcccgctcga
     1441 ccgctgacgc ttgtcggtct ttccggcaat tataaagttg acacacgtac gtgttccggg
     1501 tagagtcgcg gccatgggca agacgaaccg aaacgagccg gtcagggcgc ggtcgtcgga
     1561 agcgcggtac tcgctcatgg agttccaccg ggagttcccc gacgacgccg cttgcctcga
     1621 c
//
