LOCUS       DASZYS010000001        19563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_707311, whole genome shotgun sequence.
ACCESSION   DASZYS010000001 DASZYS010000000
VERSION     DASZYS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19563
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707311"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VHF05_00005"
     CDS             complement(<1..380)
                     /locus_tag="VHF05_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HVS79355.1"
                     /translation="MKIYVKIAGMFASAVLCFGMAHVASAHVIVHPSEAGVASYQTFD
                     MSVPVEKDFPTVALKLLIPDGVTNISPTVKPGWTIDVKKDENGNATEIDWTGGSIPVG
                     ERDDFTFSGKVPAAATTLAWKAYQT"
     gene            complement(394..975)
                     /locus_tag="VHF05_00010"
     CDS             complement(394..975)
                     /locus_tag="VHF05_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79356.1"
                     /translation="MRKKTGKLLAALVSLAAFAAAAGIIAPSASAHTTETNAGTSVLL
                     HVDPDDDPIAAQPANIILIYQNGTSTLDLSTCQCAASVEQNGQTLLSEDFSSSIDVAI
                     AGGSAEFPFTFPAKGVYEITVEGKPKAGAAFEPFSVTFDQRVDRAAPAPAAIPWWKRF
                     PETRSLNIAIAFIVLALAAFFVYRDARRRKFNK"
     gene            1048..1410
                     /locus_tag="VHF05_00015"
     CDS             1048..1410
                     /locus_tag="VHF05_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HVS79357.1"
                     /translation="MHEIFRKIAHSISEAVGTPLAFIIALLLIITWAVSGSYFHYSNT
                     WQLMINTGTTILTFLMVFIIQNTQNRDSRAIHLKLDELIRASRPAKDKFVDLEDLPDE
                     ELERLQKEFEHLRARRRH"
     gene            1462..2313
                     /locus_tag="VHF05_00020"
     CDS             1462..2313
                     /locus_tag="VHF05_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HVS79358.1"
                     /translation="MNSLIRLAERILIFAIGIFAIWAIVTQIYMPIHREVARGWAIAL
                     TYFVAAYIFIPLLIRITVAILRRKHIPRFTFTADGLPADPVFMVLHGTEKELRRAFRE
                     AGWTEADPLSWKSGAKMVITFLANRPYPEAPFSSLYLFGRRQDYGFQRSIGNSPRKRH
                     HVRFWAANIDPQEATDIKYWTQKHVIDSSKPLYWVGAGTEETGFGFARLTYQVAHAAR
                     MHIDAEREFIFSSLKVAGWVSREEYFDPGHPIIGKFISDGKILVADLASSAPQMRAVP
                     MPQEARR"
     gene            complement(2288..3664)
                     /locus_tag="VHF05_00025"
     CDS             complement(2288..3664)
                     /locus_tag="VHF05_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVS79359.1"
                     /translation="MIETPCGTRKVKRKTMKSKILRYDILTWAAFGLTLIILILSAVF
                     SYHNFKQLQEKDALVDDAHGMLDGLDSLLADLDVSVGAERAYLLTGNQAYFSSYESAT
                     QRIDADLDTLTAFASEDPERLRGLSELRPLVTERLAGLGNGIAAREEASSIVIPDALQ
                     TGAGEAVTSRIVSLAGAMRQSEVANLATREAAAAGSYAKLATTIASVTLLDLLLLFAT
                     VYFFYRYERQNAALARDKDEFIGRASHELKSPLTTQTLYLHMLQRFALQKNDVDLAVI
                     IGKIQEHTTDLTNLVTDLLDLSRMQLGTFDIHRKEDVDLDGIVHDILDGLQAIYLNRA
                     FLLHGEITCCVPGDPERIGQAITNILMNAVKYSPADQPVEVDLREVGDEVLVSVRDHG
                     IGIDSTHHVSIFKRFYRVSGISEETFPGLGIGLYISSKVIERHGGRITVESAPGKGSE
                     FTVALPAA"
     gene            3868..5571
                     /locus_tag="VHF05_00030"
     CDS             3868..5571
                     /locus_tag="VHF05_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79360.1"
                     /translation="MRNLAILAVCLALAWPLHATPSRIADTEQSLPNAKERNVVVKIF
                     STYPMDFFKFFGHPIRKTKVAGNFLKDKALRPAARHVGMPALQIARTTLPFACLTGIG
                     TIPCIGGQLALNAPSTMMMVRSFAGGAPQIRAAKFTRVKISRSSVEESSRGRYTIDNW
                     ETADVYVFDPGHAEYDPKDRQFFTGYQSVQADQIGWANQEVWNEIPGKGSVQVRLDDP
                     RIFYNGRYAYLTGCEYKGKVWFNRIRLVHPLPPAEPQVTQAPPPPAAPPVVNVNQPPF
                     PNPLNVMATVSGLQPAQIPTNYTVHVVNENIQRCGDNWCRWQKFGTGLLGPAAIAADA
                     VFPWAMTRSAHIQADGQRDAAARRADGEVAAANAAGPYLVQAAQARAQGDIGNGNAIA
                     QGTRDAGVSIQNGLNAQGTSIQSGLNNVGTSIENSGMTISNGMTSAAKARVPDNITQT
                     VTNTNSATGVNNPVNTFTPTNTFNPTNNNNVTANGGNGGSVGNISVTAKGGNAVANSS
                     SKSSASSNAEINNSGNSATTVNVPAPKPQPAPKPAPKPQPKPPAKDCPKPPPPPAQCK
                     K"
     gene            6292..6477
                     /locus_tag="VHF05_00035"
     CDS             6292..6477
                     /locus_tag="VHF05_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79361.1"
                     /translation="MKYPDFVKELIDRFGEDRGPLMALRAEVGFLRRYLERQSGPEFL
                     KDQKAMIEDFLARHPKP"
     gene            complement(6713..6784)
                     /locus_tag="VHF05_00040"
     tRNA            complement(6713..6784)
                     /locus_tag="VHF05_00040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6749..6751),aa:His,seq:gtg)
     gene            complement(6992..7073)
                     /locus_tag="VHF05_00045"
     tRNA            complement(6992..7073)
                     /locus_tag="VHF05_00045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7037..7039),aa:Leu,seq:cag)
     gene            7382..7465
                     /locus_tag="VHF05_00050"
     tRNA            7382..7465
                     /locus_tag="VHF05_00050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7416..7418,aa:Leu,seq:gag)
     gene            complement(7697..8113)
                     /gene="rplS"
                     /locus_tag="VHF05_00055"
     CDS             complement(7697..8113)
                     /gene="rplS"
                     /locus_tag="VHF05_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVS79362.1"
                     /translation="MEIVISPVKIEERRKLDLRAGDTVKVHQKIQEKGKTRLQMFEGL
                     VLAVKHGKESGGTFTVRRVASGVGVEKIFPLYSPLIDKIDVVKRSKVRRAKLYHIRKK
                     AAKEIRRQMRNVRVVAEAEEEAEEAAPEAPATETQE"
     gene            complement(8145..8903)
                     /locus_tag="VHF05_00060"
     CDS             complement(8145..8903)
                     /locus_tag="VHF05_00060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVS79363.1"
                     /translation="MNLDQIFEKIVILYEDDELLAIDKPSGLVVHSDGRTDEPSVADW
                     LERVRPEIKDVGEPWTRPDGTTLHRPGIVHRIDRETSGVLLICKTQDLFLRMKEKFKK
                     REVGKSYLAFLYGELRTNEGVIDRPIAKSRKDFRLWSAQPGGRGEMREAATEYRVIER
                     GSGFTYVEARPKTGRTHQIRVHFKAINYPIVGDRLYAPKREMALGFKRLALHAAKISF
                     TLENKGAMVIEAPIPDDFQRAEAAMTGRTGESVA"
     gene            complement(8905..9192)
                     /locus_tag="VHF05_00065"
     CDS             complement(8905..9192)
                     /locus_tag="VHF05_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVS79364.1"
                     /translation="MVLDIDIEVEIILTGKVQGVYLRDYLKKRADELGVRGVARNEDD
                     GSVSVIAQGTREALEEFLRRAKRGSVFSKVEDAEVTWYDSVGDPHIEFSIE"
     gene            complement(9196..9861)
                     /locus_tag="VHF05_00070"
     CDS             complement(9196..9861)
                     /locus_tag="VHF05_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79365.1"
                     /translation="MKTKEREKAILLRKKGESINVIAHTIGVSKASVSTWVRDISLTQ
                     RQRNQLIQNQFNIYAIEKRRKSRLSNELAKRQTIIDEAKKDIKSISKIELKLIGCMLY
                     WAEGRKRGQRVVSFSNSDPMIIRVIMRFFREICFVSEAKFRCHIHTYSHVNVSKAEKY
                     WSDITGLPLNQFYKTYSKPSKSSQSKMDILPYGTLDVDVCDAKLFLTIIGWIEKIYTL
                     VLD"
     gene            9959..10031
                     /locus_tag="VHF05_00075"
     tRNA            9959..10031
                     /locus_tag="VHF05_00075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9992..9994,aa:Val,seq:tac)
     gene            10092..10163
                     /locus_tag="VHF05_00080"
     tRNA            10092..10163
                     /locus_tag="VHF05_00080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10125..10127,aa:Arg,seq:tct)
     gene            complement(10492..10812)
                     /locus_tag="VHF05_00085"
     CDS             complement(10492..10812)
                     /locus_tag="VHF05_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79366.1"
                     /translation="MMELDVKKIFARLRSSVAGKGESPFERDWKAIIVCTLVLNICVI
                     GASLYVFWLSNNIGTEKAQASKPKIEAFNQNGFDDLESYFDARKVDFNSFQSKKPEVS
                     DPSR"
     gene            complement(10809..11366)
                     /locus_tag="VHF05_00090"
     CDS             complement(10809..11366)
                     /locus_tag="VHF05_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79367.1"
                     /translation="MKLFAGTKKTIIVLGVLLIALVAGYVFAFRFLLGENDQVDTLNQ
                     QAASYVQTNAILSSLQSTFTKTDDDAKAIDSHFVPANGVVDFITEIESLGKDSGLSLT
                     VNNVDAADIDKASSAFEERLSLRVETEGTWADTEQFIGMVENLPVGISIDGASLDALK
                     DEEGVSAGAPTHWKGEFQLSVLKLK"
     gene            complement(11363..11896)
                     /locus_tag="VHF05_00095"
     CDS             complement(11363..11896)
                     /locus_tag="VHF05_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79368.1"
                     /translation="MFNLLPYKEKKAIGAEYMRRRLIIILLFSIATVILAGVFLIPSF
                     IIAGYRLSEIKQTVAVLQANDAIKNQQTYKDAIAAANKKIATFGLSSTTEPLPELLEG
                     IAAVKSDAITITSFNASRTGGTLALTVEGTAKDRESLLSFANLLKGSGVYGKVDLPIS
                     NFAKDENISFSLNVTTK"
     gene            complement(11889..12902)
                     /gene="pilM"
                     /locus_tag="VHF05_00100"
     CDS             complement(11889..12902)
                     /gene="pilM"
                     /locus_tag="VHF05_00100"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HVS79369.1"
                     /translation="MAPSKFFHFFPVPSFLRMPAAGLSVNDEGVRFVHISDIDGAAKV
                     ESFGSADFPAGAVVNGEIRDEAAVANAFAALKKQYGLRFVRAAISDDAVYLFRLNLPL
                     ISESDVRSAIEFKLEENVPIPAGDAIFDYSIANRNDVLGTTEAVVAAVASKTSESYLD
                     TMKSAGLSPLRLDLESQAIAHAVVPKAERGTVLVAHFAHTKTLLFLVQGGMVQFSFTL
                     PVEERADPAGAQSPQKYQKLVLVREGIERIVNYWKDTMPLPAGESFSFKVIASGGEAL
                     DAEFVSKFASLAGQPVEVADPWQNVFDSNDAAPPIPKEDSFRYAAAIGVALANCPRLN
                     FHV"
     gene            complement(12947..13273)
                     /locus_tag="VHF05_00105"
     CDS             complement(12947..13273)
                     /locus_tag="VHF05_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79370.1"
                     /translation="MINIDKFHETEIRIGKILSAEKVPETDKLLRLAVDFAEENPRQI
                     VSGISAYFPDPQTLVGRHVAFVANLEPRTIKGLESQGMILAAHEGEAFSLLEVSSDIP
                     PGTRVG"
     gene            complement(13392..13772)
                     /gene="ruvX"
                     /locus_tag="VHF05_00110"
     CDS             complement(13392..13772)
                     /gene="ruvX"
                     /locus_tag="VHF05_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVS79371.1"
                     /translation="MRYLGIDFGSKRVGIAVSDEEGTMAFPKAVLENTSDLAAAVKKI
                     CEENGVEKIILGESKNYRGEYNPIMEKGFVFKEELARETGLPVELEPEFLTSAQAERG
                     SSPDMLDAGAAALILQSFLDRINK"
     gene            13983..14258
                     /locus_tag="VHF05_00115"
     CDS             13983..14258
                     /locus_tag="VHF05_00115"
                     /inference="COORDINATES: protein motif:HMM:NF039180.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HVS79372.1"
                     /translation="MESLDASTILTLVGVLAWVWVWKGIALWKSARLSHRNWFLAFII
                     FNIYTFGILEIIYIFLIASKYKVETSETEEAATPTNKSGSGENSDEQ"
     gene            14269..14353
                     /locus_tag="VHF05_00120"
     tRNA            14269..14353
                     /locus_tag="VHF05_00120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14303..14305,aa:Ser,seq:tga)
     gene            14970..15434
                     /locus_tag="VHF05_00125"
     CDS             14970..15434
                     /locus_tag="VHF05_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79373.1"
                     /translation="MAYKPLEWEAQESVHTEKSSDWYWAVGIITIAAVVTCVILGNIL
                     FGLVILLGSAGLTMHAAKEPPLHYYKLDEHGIHIDDTYYPYRYLDSYWIDENYYPVKA
                     LLKSKKFFMPYLVMAIPDNVDIELVRKHFDHHLPAHEHHEPLPHKILEYLGF"
     gene            complement(15639..15710)
                     /locus_tag="VHF05_00130"
     tRNA            complement(15639..15710)
                     /locus_tag="VHF05_00130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15675..15677),aa:Arg,seq:ccg)
     gene            15811..15882
                     /locus_tag="VHF05_00135"
     tRNA            15811..15882
                     /locus_tag="VHF05_00135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15844..15846,aa:Arg,seq:gcg)
     gene            complement(16093..16359)
                     /gene="rpsT"
                     /locus_tag="VHF05_00140"
     CDS             complement(16093..16359)
                     /gene="rpsT"
                     /locus_tag="VHF05_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVS79374.1"
                     /translation="MPITRSAKKALRNSANKQEHNLARKGALKSALKKMRKLVVEKKA
                     DEAKKFLPEVQRVIDKAAKTGVIKKNNAARKKSRIVAALKKASA"
     gene            16455..17042
                     /gene="ruvA"
                     /locus_tag="VHF05_00145"
     CDS             16455..17042
                     /gene="ruvA"
                     /locus_tag="VHF05_00145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVS79375.1"
                     /translation="MISHLRGVVEQKGLNFAVLDVQGVGYRVFMTSGLLAELAIGEEA
                     KIPTYLAVREDAMDLYGFRNQDELRFFELLISISGIGPKSALSILNITTLDSLRQAVA
                     SEDTTHLTRVSGIGRKTAERIVIELRGKLDGFEFEAIATDTGSIKEEVDALEALKSLG
                     YSQKESQEALKLVPKDIIGTGEKVKHALRLLASNQ"
     gene            17054..18391
                     /gene="murE"
                     /locus_tag="VHF05_00150"
     CDS             17054..18391
                     /gene="murE"
                     /locus_tag="VHF05_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HVS79376.1"
                     /translation="MNPSNILEKTLRRAKRLIPKSLFRAAQPFYHYALAILGALRYGF
                     PSKKLMVIGVTGTKGKSTTVELVNSILEAAGYKTAVAGTIRFKIGDDSRRNMYKMTMP
                     GRFFLQSFLARAVKAGCTHAVIEMTSEGSKQYRYRFVYPDALIFTNLSPEHIESHGSY
                     EKYVEAKLDIARRVLRSGKPRRILVANGDDKETPKFLGLGIPESLTYFLSATPHVSSE
                     SGSTFTFMGTEMRTPLPGEFNLANALGAATLAQALGIDTAAIALGIKNLAHVPGRAET
                     VTLAEDHELAREQDFTVVVDYAHTPDSLKAIYGAYGAHRKICVLGSTGGGRDTWKRGA
                     MGAIADEYCDEIILTDEDPYDEDPQKIVADVAEGIKSKKPDMLMDRREAIHAALSRAR
                     KGDAVLITGKGTDPYIMGKNGAKQPWSDANIAREELEKVLKSRASQQKSEPQE"
     gene            18433..18831
                     /locus_tag="VHF05_00155"
     CDS             18433..18831
                     /locus_tag="VHF05_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HVS79377.1"
                     /translation="MPHVSKKNISNKISLKIEERLLRVVTGLNSKKSAMLFELLTITE
                     KTMLAKRLGIVFMLTEKRSFYEISEKLGVSTSTVSRIEDDMKRGAFKRLRIYFEKHRE
                     GFWNSLEKFMRAGMPPIVGKGRWDYLKEMP"
     gene            18917..>19563
                     /locus_tag="VHF05_00160"
     CDS             18917..>19563
                     /locus_tag="VHF05_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVS79378.1"
                     /translation="MPELPEVQTTVNGVKRYAKGKRIVDVWTDYGSPFHEGKSNIKNR
                     KYFARFKKEVAGRSITGAERIGKNVIIHLSGGKSIVVHMKMTGHLLFGEYAFDGKTWT
                     AKHAGPLRDDGFNRFIHLLFTLSGGKHLALSDMRKFARVSVVETSSLPHADDLDSIGP
                     DPLNRDFTYKVFKGRLLKRPAGKIKQALMEQKLIAGIGNIYSDEILWRAGVHPLS"
BASE COUNT         4564 a         5361 c         5138 g         4500 t
ORIGIN      
        1 gtctggtacg ccttccacgc gagcgtcgtt gcggcggcgg gaaccttgcc gctaaaggtg
       61 aagtcgtcgc gctccccgac cggaatcgag ccgccagtcc agtcgatctc ggtcgcattg
      121 ccgttctcat ccttcttgac gtcgatcgtc cagccgggct tcaccgtggg agaaatattg
      181 gtcactccat cgggaatgag gagctttagg gccacggtcg gaaagtcctt ctctaccggc
      241 acgctcatgt cgaacgtctg atacgacgcc accccggcct cgctcggatg cacgatgaca
      301 tgcgccgagg cgacatgcgc catgccgaag cagaggacgg cagacgcgaa cattccggca
      361 attttcacat atatcttcat ttcgcttttt tgattattta ttaaattttc tccggcgcgc
      421 gtcgcgatat acgaagaacg cggcaagggc gagaacgata aacgctatcg cgatattgag
      481 gctcctggtc tcaggaaacc gcttccacca aggaattgcg gctggtgctg gcgcggcgcg
      541 atcgacgcgc tgatcgaacg tgacgctgaa cggctcgaat gccgcgccgg ccttgggttt
      601 tccttccacc gttatttcat acacgccctt cgcggggaac gtgaatggaa attcggcaga
      661 tccgccggca atggcgacat cgatcgacga cgagaaatcc tccgagagga gcgtctgccc
      721 gttctgttcg accgaggcgg cgcattggca cgtcgagagg tcgagcgtgc tcgtgccgtt
      781 ctggtatatg aggataatat tcgccggctg cgccgctatc gggtcatcgt ccggatcgac
      841 atggagaagg acgctcgtac cggcattcgt ttccgtagta tgcgccgaag cggaaggcgc
      901 tattatcccc gccgcagcgg cgaatgccgc caaggagacg agagcggcaa gcaactttcc
      961 cgttttcttc ctcatgagcc ctagtatacc agataggtta tgcacattcg cccgctccag
     1021 ctccggcctc cgggatataa tggatccatg cacgaaatat tcagaaaaat tgcgcactcg
     1081 atcagcgagg cggtgggtac gccgctcgcc ttcattatcg ctctcctcct catcattacc
     1141 tgggcggttt ccggctcgta cttccattat tccaacacct ggcagctcat gatcaatacc
     1201 ggcaccacca ttctcacctt cctcatggta ttcatcattc aaaacaccca gaaccgcgat
     1261 tcgcgggcga tccacctgaa gctcgacgag ctcatcagag cgtcgcggcc ggcaaaagac
     1321 aagttcgtcg accttgaaga ccttcccgac gaggagcttg aacggctcca gaaggagttc
     1381 gagcacttga gggcccgcag gcggcattga tgcgaatgga ggcttgtctc cgccgccggc
     1441 cctgccgtac aatgtatttt catgaattcc ctcatcaggc tcgctgagcg catcctgatt
     1501 tttgcgatcg gtattttcgc catatgggcg atcgtgacgc agatctacat gccgatccac
     1561 cgcgaagtcg cccgcggctg ggcgatcgcg ctcacgtatt tcgtagccgc ctatattttc
     1621 atccccctac tcattcggat caccgtcgcg attctgcgcc gcaaacacat tccccgcttc
     1681 accttcaccg ccgatgggct tccggccgat cccgtattca tggtacttca cggcaccgaa
     1741 aaagagcttc gacgggcgtt ccgggaagcg ggctggaccg aggccgatcc cctttcatgg
     1801 aaaagcggcg cgaaaatggt catcaccttt ctcgcgaaca ggccctaccc cgaagctccg
     1861 ttcagctcgc tctatctttt cggaaggcgg caggattacg gcttccagcg gtcaatcggc
     1921 aacagtccgc gcaagcgcca tcatgtccgc ttctgggccg caaacatcga cccgcaggaa
     1981 gcgacggata ttaaatactg gacgcagaag cacgtgatcg attcatcgaa gcccctctac
     2041 tgggtcggcg cggggacgga ggaaaccggc ttcggtttcg cgcggcttac ctaccaggtc
     2101 gctcatgccg ctcgcatgca catcgatgca gagcgcgaat ttattttctc ttcgctcaag
     2161 gttgcgggct gggtatcgcg cgaagaatac ttcgatccgg ggcacccgat aatcggcaaa
     2221 ttcatttccg acgggaaaat tctcgtagcc gatctcgctt cttcagctcc gcagatgcgg
     2281 gccgtcccta tgccgcagga agcgcgacgg taaattccga tcctttcccg ggcgcgcttt
     2341 cgactgtgat cctgccgcca tggcgctcga tcactttcga ggagatatag aggcctatgc
     2401 cgaggcccgg aaacgtctcc tcgcttattc cgctcacgcg gtaaaaacgc ttgaagatcg
     2461 acacgtggtg cgtcgagtcg atgccgatgc cgtgatcgcg gacgctgacc agcacttcgt
     2521 ctccgacctc ccgcaaatcc acctccacag gctggtccgc cggagaatac tttaccgcat
     2581 tcatgaggat attggtaatg gcctggccga tacgctcggg atcgccggga acgcagcagg
     2641 tgatctcgcc atggagcaaa aacgcgcggt tgagatagat cgcttgcagg ccgtcgagga
     2701 tatcgtgaac aatcccgtcg agatcgacat cctctttcct gtgaatatcg aacgtgccga
     2761 gctgcattct cgagagatcg agcaggtcgg tcacgagatt ggtgagatcg gtcgtgtgct
     2821 cctggatctt gccgatgatc accgcgagat cgacgtcgtt cttctgaagc gcgaagcgct
     2881 ggagcatgtg aagatagagc gtctgcgtcg taagcggcga cttgagctca tggctcgcgc
     2941 ggccgatgaa ttcatccttg tcccgcgcaa gagccgcgtt ctgccgctcg tagcgataga
     3001 agaaatagac cgtcgcgaag agcagtagga ggtcgaggag cgtcaccgaa gcgattgtgg
     3061 ttgcgagctt ggcgtaactt cccgcggcgg ccgcttcgcg agtagcgagg ttcgcgacct
     3121 ccgattgcct catcgcgccc gcaagcgaga ctatcctgct cgtcacggcc tcgcccgcgc
     3181 cagtctgaag agcgtccgga atcacgatgc tgctcgcctc ttcccgcgcc gcgatgccgt
     3241 tcccgagccc ggccaagcgt tctgtcacga gcggacggag ctccgaaagc ccccgaagcc
     3301 tctccggatc ttccgaagcg aacgcggtaa gcgtatcgag gtccgcatcg attctctgcg
     3361 ttgccgattc atagcttgaa aaatacgcct gattgcccgt aagaagatat gcacgttctg
     3421 cgcccaccga cacgtcgaga tcggccagaa ggctgtccaa gccgtccaac atgccatgcg
     3481 cgtcgtcgac gagcgcgtct ttctcctgaa gctgtttgaa attgtggtac gagaaaacgg
     3541 cacttagaat aagaattata agcgtcaggc cgaaggccgc ccatgtcaaa atgtcataac
     3601 gaagaatctt tgatttcatc gtcttcctct ttaccttccg tgtgccgcac ggcgtttcga
     3661 tcatacgaaa actggatgaa aaaaacggta cgagaaaatc gcccgtaaaa tcggcatttt
     3721 tccataattg ggggccataa ctcggaatac gatgtcgccc taccattgac tttcatctga
     3781 atttagtgca atatcgccgg agatgtagaa gtcctttatc atagaaattc caacaattca
     3841 gacgtcaaaa gaaaggaagg tagcagtatg aggaatctcg cgattcttgc agtatgcctg
     3901 gcgctcgcct ggccgcttca cgcaacgccg tcgcgcattg ctgacacgga acaatcgctg
     3961 ccaaatgcaa aagaaaggaa tgtggtcgtg aaaatcttct cgacgtaccc gatggatttt
     4021 ttcaaattct tcggtcatcc gattcgaaaa acgaaagttg ccggaaattt tctcaaggat
     4081 aaggccttga ggccggcggc ccgacacgtg ggaatgcccg cgcttcagat cgcgcggaca
     4141 acgctcccct tcgcatgcct caccggcatc ggaactattc cctgcatcgg cggccaattg
     4201 gcgctcaatg cacccagcac gatgatgatg gtccgcagtt tcgcgggcgg cgctccgcag
     4261 atcagagcgg cgaaattcac gagagtgaag atcagccggt catcagttga ggaatcatct
     4321 cgcggacgat atacgatcga caactgggaa acagccgacg tatacgtctt cgatcccggc
     4381 catgcggaat atgatccgaa ggaccggcaa ttctttaccg ggtatcaaag cgtacaagcc
     4441 gaccagatcg gttgggcgaa tcaggaagta tggaacgaga ttcccggcaa gggttcggtc
     4501 caggtacgcc tcgatgatcc gcggatcttc tacaacggaa gatatgcgta tctcaccggc
     4561 tgcgagtaca aaggaaaagt gtggttcaac aggattcgcc tggtgcaccc gcttcctcct
     4621 gcagagccgc aggtaacgca agctcccccg cctccggcgg cgccgcctgt cgtcaatgtg
     4681 aatcagccgc cgttcccgaa tccgctcaac gtaatggcga cggtaagcgg ccttcagcca
     4741 gcgcaaatcc cgaccaatta caccgtgcat gtggtgaatg aaaacattca gcgatgcggc
     4801 gataattggt gcagatggca aaaattcggt actggtcttc ttgggcccgc ggcaattgcg
     4861 gcagacgcag tatttccttg ggcaatgacg cgatccgcgc atatacaagc ggacggacaa
     4921 cgtgacgcgg cggcaagaag agctgatggc gaagtcgcag cggcaaatgc cgcaggtccg
     4981 tacctagtgc aagcagccca agctcgcgcg cagggtgata tcggcaacgg aaatgcgata
     5041 gcgcagggca ctcgggatgc cggcgtttca atccagaacg gattgaatgc ccaaggcact
     5101 tcgatccaaa gcggattgaa caatgtcgga acctcgattg agaattccgg aatgacaatc
     5161 agcaacggca tgacaagtgc cgctaaagct cgcgtgccgg acaacatcac gcagaccgtc
     5221 acgaacacga attcggcgac cggcgtgaac aatccggtaa acacgtttac tccgaccaac
     5281 acgttcaatc cgacgaacaa caataacgtc actgcgaacg gcggaaatgg cgggagcgtc
     5341 ggcaacatca gcgtcactgc caagggcggc aatgctgttg ccaactcaag ctcgaagtcg
     5401 agcgcgagtt cgaatgccga aataaacaac agcggcaact cggccactac ggtcaacgtg
     5461 ccggcaccga agccgcaacc ggctccgaaa ccggctccca agccgcagcc gaagcctccg
     5521 gcaaaggatt gcccgaagcc tccgccgccg cctgcgcaat gcaagaaata gccgcgcagg
     5581 attaggcaga aagaaacaaa gcggcccgcg ggacgctcta ttcccgccac cacgcataca
     5641 ccacctgacg tctgacattg ttcaggggta ttaccttcgc aagcttcgta tctcgagaac
     5701 gaggcttgct tttttatatg caaaattatt tcgattcttt tttaattgac atatcaaaaa
     5761 attatacgta gtatccacat actgtccttt gatataaatc aaaggtagga tagtttcaga
     5821 gagaggaaaa ccatggaagt cagttttgcg gccgacttga aagagttggt taaagaatcg
     5881 gataacttta tccttccagc atgcaactcc tttgtaaggg ttgtaacgct caccatcgtc
     5941 tacgtcctta tgggaatcgg ctcaaccata ttgctcgctt ccccagcaca aatcagaacc
     6001 aatgatccaa tatggacact ttcagcgatt tggattgctg taatcctgcc aatcgcacgt
     6061 gccacatatc tttggcatcg ttcaaggaaa aggagggcgg caatcaacct tctatacgag
     6121 gaatcggtgc accaacccag ctctttatga tggattatgt acgacacgac tgctccggcc
     6181 taaatgaccg gagcaatttt tattatcgca aaagcatttc ttccatactc ccaacgaggc
     6241 tttgcgtggg agaattatct ttaagaagaa tgggcatgct aaaattccca tatgaaatat
     6301 ccagatttcg ttaaagaact catcgaccgc ttcggagaag accgaggacc gctcatggcg
     6361 ctccgcgccg aagtgggatt cctgcggcgt tacttggaac ggcagagcgg gccggaattt
     6421 ctcaaggatc agaaagcaat gatcgaggac ttcctcgccc ggcatccgaa accataataa
     6481 aaccaccttt aaggtggttt tattatggtt gaaatctttt taattactgt ctggggtgcc
     6541 tatcgggaac aggaattgat ccttacagga tctgtacacc tttcgtattc acttcgccaa
     6601 aagactggct cgttcatata ctcaaggtct tcaattcccg aacgcgagca aaattatttg
     6661 ctcgtttagg cacccaagac gacaaaaccg ccttgcggcg gttgtcgtct tgggtgccta
     6721 tcgggaattg aacccgaatc gagagttcca caaactccag tgttaaccat tacaccatag
     6781 gcaccatttg agcctcgtaa ggcttctcag ttatatcaga aattaagctt caaagtctac
     6841 ctgtgggcac ggggagactc ggtcacggat aattttctgc tgaaaatttc cgcgaccccg
     6901 gctcggcccg tcggcctccg cctttctcgc ctcccctgcc tcgcaatgat gaataactcg
     6961 ggcagctccc agctgtcctc gttaatcatt gtgggcacgg ggagactcga actcccaacc
     7021 cttgcgggat acgcttctga gacgtacgcg tataccagtt ccgccacgtg cccatgcgga
     7081 tgtattatag ccggaattga attctattcc agagctggag aattttagaa aaccagggcc
     7141 ggccagcggg tggcgaccag gataaacgta tgcaggtcgt cgggagatga tgccaagtgg
     7201 ctattcggca cgcctgaatg ccactgccac cagaatacga cgccgacaac gcagtagagg
     7261 aacaaacaaa ggagaagcca gccgaacaag gtgcgcatgg gttaagaata gcacgggacg
     7321 ggaccggact tgaaaagata gaggctttta ggcatactta ccttgttccc aaattaggaa
     7381 tgcgcaggtg gcgaaattgg cagacgcact accttgaggt ggtagcgccc gtaagggcat
     7441 ggaggttcaa gtcctctcct gcgcacaaaa agaaagaacc cggctaaagc cgggctttct
     7501 ttttgtgcgc agagcaagac gcctgcgcgt cttgcatgag aggacttgaa gaccttgagc
     7561 atattttcga gcggccttcg cgtgagaaaa tgcgaaaggt gtacagatcc tgtaaggatc
     7621 aagactcctc tcctggcgaa caaaatattc ctgaccacaa aataaaaaaa caactcccat
     7681 ctgggagttg ttttcattat tcctgagttt cggttgccgg agcttcggga gcggcctctt
     7741 cagcctcctc ttccgcttcc gcgacgacgc ggacattacg catctggcgg cgaatttcct
     7801 tagccgcctt cttcctgatg tggtagagct tggcgcggcg gaccttcgag cgcttcacta
     7861 cgtcgatctt gtcgatcaga ggcgagtaga gcgggaagat tttctcgacg ccgacgccag
     7921 aggccacgcg gcgcacggtg aacgtgccgc ccgattcctt accgtgcttc acggcgagga
     7981 cgaggccttc gaacatctgg aggcgggtct tgcccttctc ctggatcttc tggtgcacct
     8041 tgacggtgtc gcctgcccgg aggtcaagct tcctgcgctc ttcgatcttt actggcgaga
     8101 ttacgatttc cataacggga gttaatgtac cataggggcc aaacttacgc aacactctcc
     8161 cccgtacgcc ccgtcattgc cgcttcggcg cgctggaagt cgtcgggaat gggcgcctca
     8221 atgaccatgg cgcccttatt ttcaagtgta aacgagatct tcgccgcatg gagggcgagg
     8281 cgcttgaaac ccagggccat ttcgcgcttc ggagcataga gccggtcgcc gacaatgggg
     8341 taattgatcg ccttgaagtg gacgcgaatc tgatgggtgc ggccggtctt gggcctcgct
     8401 tcgacgtacg tgaagccgct cccccgctcg atgacgcgat actccgtggc cgcctcccgc
     8461 atttctcccc ttcctccggg ctgtgccgac cagaggcgga aatccttccg gcttttggcg
     8521 attggccgat cgatgacgcc ttcattcgtc cgcaattcgc cgtagaggaa cgcgagatag
     8581 cttttcccga cttctctctt tttgaatttc tctttcatgc ggagaaagag atcctgcgtc
     8641 ttgcagataa ggagcacgcc cgaggtctcg cggtcgatgc ggtgaacgat accggggcgg
     8701 tggagcgtcg ttccgtcggg acgggtccac ggctcgccga catccttgat ttcggggcgc
     8761 acccgctcga gccagtcggc gaccgacggc tcgtccgtac gtccgtcgga atgaacgacg
     8821 aggcccgacg gcttgtcgat cgcgaggagt tcgtcgtctt cgtagagaat gacgatcttt
     8881 tcgaaaattt ggtcaagatt cattttattc aatggagaat tcgatatgcg ggtctcctac
     8941 cgaatcgtac caggttacct ccgcatcttc caccttcgaa aagacgctcc cccgcttcgc
     9001 gcgccgaaga aactcctcga gcgcctcgcg cgtgccctgc gcgattaccg agaccgagcc
     9061 gtcgtcctcg ttgcgtgcga cgccccgcac gccaagctcg tcggcgcgct ttttcaggta
     9121 atcgcgcaga tagacgccct gaactttgcc ggtgagaatg atttcgactt caatgtctat
     9181 atctaaaacc attttttaat ctaacactaa ggtataaatt ttctcaatcc acccgattat
     9241 cgttagaaac aattttgcat cacatacatc cacatctaac gtaccgtaag gcaaaatatc
     9301 cattttactc tgacttgatt tactaggttt tgaataggtt ttataaaatt gatttaaggg
     9361 aagacctgta atatcggacc aatatttttc tgccttgctt acattcacat gagaatatgt
     9421 atggatatga catctaaatt ttgcttccga tacaaagcaa atttctcgga aaaaacgcat
     9481 tatcacgcga ataatcatgg gatcactatt agaaaaactt acaacccttt gtcctctttt
     9541 tcttccttcc gcccagtaaa gcatgcaacc gatgagcttg agctcgattt ttgagattga
     9601 ttttatatcc ttttttgcct catcaattat agtctgacgt tttgctaatt cattagatag
     9661 tctgcttttt cgcctctttt ctatagcgta gatgttaaat tgattttgaa taagctgatt
     9721 tctttgtctt tgtgttaagg atatatctct cacccacgtg cttacacttg ctttggaaac
     9781 tcctattgtg tgcgcaatca cattaatcga ttcgcctttt ttgcgcagta aaattgcttt
     9841 ttctctttct tttgttttca tccaatttgg agtatgtttt atatactata tattaccata
     9901 gttcatattt taagcagttg ttgataaaac ctacgaaata gaataatatt tgccacacgc
     9961 gcgattagct cagttggtag agcaatcgat ttacactcga aaggtcgggg gttcgagtcc
    10021 ctcatcgcgc acacgaacga aaatctgtca tttttataat cccgaaactt tgctaatgta
    10081 atagcgttac tgcccttgta gttcaatgga tagaactggg aacttctaat tccccgatgt
    10141 aggttcgatt cctaccgagg gcacccgacc ataaaaacac cggttttccc ggtgttttta
    10201 tgtgtcgctg gaagtatcgg taggaatcga aagccggagc gcgacggcgc gaggcggggt
    10261 cgcgggaatt ttcagcagaa aattatccgt gaccgattcc taccgagggc acattgaggc
    10321 gaaaaaacag tgcctgcgcg ctgttttttc gcgaaatgtg aggcggagcg atgccgagtt
    10381 agcaaacgag gccgcgagcc cgggtcggga aattttgcga gcgacggcga ggaaaatatt
    10441 cctgaccaat ttattaggaa cagatgataa ttaaaattct ttccaccaat tttatcttga
    10501 cgggtcgctc acttccggct tcttcgattg aaagctgttg aaatcgactt tcctcgcgtc
    10561 gaaatacgat tccagatcat cgaagccatt ctggttgaac gcctcgatct tcggcttcga
    10621 cgcctgggct ttttcggtgc cgatattgtt cgagagccag aaaacgtaga ggctcgcgcc
    10681 gatgacgcag atattcagaa cgagcgtgca gacgatgatc gccttccaat cgcgttcgaa
    10741 cgggctctcc ccttttccgg cgacggagga cctgagccgt gcgaatattt ttttgacatc
    10801 gagttccatc atttgagttt gagtacgctt aattggaatt cgcctttcca gtgggtcggc
    10861 gccccggcgc tcacgccctc ctcgtccttg agcgcgtcga ggctcgcgcc gtcgatcgag
    10921 attccgacgg gaagattctc aaccatgccg ataaactgct cggtatcagc ccaggttccc
    10981 tctgtctcga cgcggagtga caagcgctcc tcgaatgcgg agctcgcctt gtcgatgtcc
    11041 gccgcgtcga cattgtttac ggtaagcgag aggccgctgt ccttgccgag cgattctatc
    11101 tcagtgatga aatcgacgac gccgtttgcg ggtacgaaat gcgaatcgat cgccttcgcg
    11161 tcatcgtcgg tcttggtaaa agtcgactgg agagacgaga ggatcgcgtt cgtctgcaca
    11221 taggaagcgg cttgctgatt gagcgtgtca acttggtcgt tttcgccgag caggaacctg
    11281 aacgcgaata cgtagcccgc aacgagcgcg atgagaagca cgccgagcac gatgatcgtc
    11341 ttttttgttc cggcaaaaag cttcatttcg tagttacgtt aagcgaaaag ctgatattct
    11401 catccttggc aaaattcgat atcggcaggt cgaccttccc gtacaccccg cttcccttga
    11461 gcaggttcgc gaacgaaaga agcgattcgc ggtcttttgc cgtcccttcg accgtgagcg
    11521 cgagcgttcc gccggttctc gacgcattaa acgaggtgat cgtgattgca tccgatttta
    11581 ccgccgcgat cccctcgaga agctcgggga gcggctcggt cgtcgatgag agcccgaagg
    11641 tggcgatctt cttgttcgcc gcggcgatgg cgtccttgta tgtttgctga ttcttgatcg
    11701 cgtcattcgc ctggagtacc gcgacggtct gtttgatctc gctcaagcga tagccggcga
    11761 taatgaagga agggatgaga aacacgccgg caagtatgac ggtcgcgatc gaaaagagca
    11821 ggatgatgat aaggcgccgc cgcatgtatt cggccccgat cgcctttttt tctttgtatg
    11881 gcaggaggtt aaacatggaa attgagcctc gggcaattcg cgagggcgac gcctatcgcg
    11941 gcggcgtacc tgaacgaatc ctctttggga atgggaggcg ccgcatcgtt tgaatcgaag
    12001 acgttctgcc acggatcggc cacttcgacc ggctggccgg cgaggctcgc gaacttcgaa
    12061 acgaattcgg catcaagcgc ctcgccgccg ctcgcgatga ctttgaagct gaagctttct
    12121 cccgccggaa ggggcatggt atctttccaa taattcacga tgcgctcgat cccctcccgc
    12181 accagcacga gcttctggta tttctgtgga gattgagcgc cggccggatc ggccctttct
    12241 tcgacgggga gcgtgaagct gaactggacc atgccgcctt gcacgaggaa aagaagcgtc
    12301 ttggtgtgcg cgaagtgcgc cacaagaacc gtcccccgct cggcctttgg gacgaccgca
    12361 tgcgcgatcg cctgggattc gaggtcgaga cggagaggcg acaggccggc gcttttcatg
    12421 gtatcgagat agctttcgga tgtcttggac gcgacggcgg caacgaccgc ttcggtcgtg
    12481 ccgaggacat cgttgcggtt tgcaatggaa taatcgaaaa tcgcgtcccc cgcgggaatc
    12541 ggaacgttct cctcgagctt gaactcgatg gcgctcctta cgtcgctctc ggaaatgagc
    12601 ggcaggttga gacggaagag atacaccgca tcgtcggaga tcgcggcgcg cacgaatcgg
    12661 aggccgtatt gcttcttgag cgcggcaaac gcatttgcca ccgcggcttc gtcgcgaatt
    12721 tcgccgttta cgaccgcgcc ggcgggaaaa tcagccgagc cgaagctctc gactttcgcc
    12781 gcgccgtcga tgtcggatat gtgaacgaag cggacgccct catcgttgac cgagagtccg
    12841 gcggcgggca tccgcaaaaa agaaggtacg ggaaaaaaat gaaagaattt gctgggagcc
    12901 atcaacaaaa ttatacatta gtttggctgc agcatcatga ggacatctag ccgacgcgcg
    12961 tccccggcgg aatgtcggac gacacttcga gaagcgagaa cgcctcgcct tcgtgcgccg
    13021 caaggatcat tccctggctc tccagccctt tgatggtgcg cggctcgaga ttcgcgacaa
    13081 acgcgacatg gcggccgacg agcgtctggg gatcgggaaa atacgccgag atgccggaaa
    13141 cgatctgccg cggattttcc tcggcgaagt cgacagcaag gcgcaggagc ttgtcggtct
    13201 cggggacctt ctcggctgag agaatcttcc ctattcggat ttccgtctcg tggaatttgt
    13261 cgatgttgat catattgttt actaaatttt caatttgcaa tttttaattt tcaatgaatt
    13321 ttcaaaatcc aggactccag tgttgaaaat tgtctaattg aaccttgatt gaaaattgat
    13381 aattgaaaat tctatttatt tattctatca agaaatgatt gcaggatgag cgctgcggct
    13441 ccggcatcga gcatgtcggg agaactcccg cgctcggcct gggccgacgt aagaaattcc
    13501 ggctcgagct cgacaggaag gcccgtctcg cgggcaagct cttccttaaa gacgaagcct
    13561 ttctccatga tcgggttgta ttcgccgcgg taattcttcg attctccgag gatgatcttt
    13621 tccactccgt tctcttcgca gattttcttc accgcggcgg cgaggtcaga ggtattttcg
    13681 agcacggctt tcgggaacgc catggttccc tcctcgtcag aaacggcaat acccacccgt
    13741 ttcgatccga aatctattcc gagataacgc atcccgttag aagcaggtcg cgatcatgtc
    13801 gctttctgct gtgcggtatt tttctattaa aaactcgacc attttatata tgttattcga
    13861 ggttattcga gcgatcgcgg cttctaacgg gacgcatgcg cctattgtac atgagcgccg
    13921 agccgtccga aagcgccgga aaagcgcttt gcaacggtgc cgctttagta tagtatggcc
    13981 gtatggagtc tttggacgca tcgacaatcc tcacgctcgt gggcgttctc gcatgggtat
    14041 gggtatggaa aggaatcgcc ctttggaaat ccgcgcgcct ttcgcacagg aactggtttt
    14101 tggcgttcat catattcaat atctacacct tcggcattct cgagatcatc tacattttcc
    14161 tcatcgccag caagtacaaa gtggaaacgt ccgagaccga agaggctgcg acgcctacca
    14221 ataagagtgg ctcaggagaa aattcggacg aacaataaat aaatatatgg aggggtggca
    14281 gagtggtcta tcgcacctgt cttgaaaaca ggagtctcgt aagggaccgc gagttcgaat
    14341 ctcgccccct ccgccggttc aaaaatgagc acctaatggt gccatttttg ttcggcggag
    14401 gagggcaaag cgcctgcgcg ctttgcgcgt gagattcgaa aagcggagcg ttattcgctc
    14461 agtagagcga atcgcgagct gggcctgcgg ccgaggcttg cgacggcaag ccgagagctg
    14521 taggcgaatc tcgccccctc cgcacataga agtaatcatt catttaagtg agtaaaatta
    14581 aaatgaaaag gccggcgatt gttagatcgc cggctgtcac atcgctccct ctttaggatt
    14641 gcgagtcggt ttttcgtcca ctagagactt attttagtac tcgctggttc gcaaggctaa
    14701 atgtgcacat tcttaccctg ctactcaggt attagcgctt ccggaagcgc tttacctact
    14761 accccattgg tagccatgaa gcatggaact atccgaatgg tttgtcctct tccctctgaa
    14821 aaagggaatc actgttgttt tcttgaagga acgatgctgg caataagtat atatcagcat
    14881 gtttgttcgt caagtgttga gttttaatat gattcaccct tctttataaa cacacgcccc
    14941 aggaaagcta tgctactata tttatgaata tggcatacaa gcctcttgag tgggaagcgc
    15001 aggaaagcgt tcacaccgaa aaaagctctg actggtactg ggccgtcggc atcatcacga
    15061 tcgccgcggt ggtcacctgc gtcattctcg gcaacatcct cttcgggctt gtcatcctac
    15121 tcggaagcgc cggccttacc atgcatgccg caaaggagcc gccactccat tattacaagc
    15181 tggacgagca cggcatccat atcgacgata cgtactatcc ctaccgctac ctcgattcgt
    15241 actggatcga cgaaaattat tatcccgtca aagcgctcct caaatcgaag aagtttttca
    15301 tgccctatct cgtgatggcg atccccgaca atgtcgacat cgagcttgtc cggaagcatt
    15361 tcgaccacca tctccccgcc cacgagcacc atgagcctct gccgcataag attttggaat
    15421 atttgggttt ctaaacacaa aaccccgcgc aagcgcaaaa tgttttttgg tcaggaatat
    15481 tttggtcaat gtatcccttg tggtcacgag tcgattttgc ctcggtcagg caaaatcgct
    15541 cacgaccccg gctcggaatt tttcgctgct gcggaaaata tacctgcgcc cagcaccttg
    15601 cgaaaaaacc tgccgctgtg caggttttct cgtctcggtg ctctcggtcg gaatcgaacc
    15661 gacattacca gcttcggagg ccagcgttct atccattaaa ctacgagagc ttcgtaggga
    15721 tactgtagca gaaaagagag tggagcggta atgccagaac agctttgatt tggcgcattt
    15781 tttgtgttag gttgataggg ctagagaaac gctcccgtag ttcaacggat agaatgcaag
    15841 attgcggatc ttgcgattga ggttcgattc ctcacgggag cacatcgaga caaaaaacag
    15901 tgcttgcgca ctgtttttcg cgagatgtga gccggaggca tatcgctctt agcaaagagc
    15961 gatgccgagg cggggtcgca gaaattcacg agtggcgacg agtgaattat ctgcgaccat
    16021 cggaaatggt tttataaccg attcttcacc aaaacaaaaa ccgccccgaa ggggcggttt
    16081 ttgtttgaat agttaggcgc ttgccttctt gagcgccgcg acgatccgcg acttcttgcg
    16141 ggcggcgttg ttcttcttga tgacgccggt cttcgcagcc ttgtcgatga ccctctggac
    16201 ctcggggagg aattttttcg cctcgtcggc cttcttttcg acaacaagct tcctcatttt
    16261 cttgagggcg gacttcaatg cgcctttgcg ggcaaggttg tgctcctgct tattggcgga
    16321 gttcctgagc gctttttttg ctgatcgggt aattggcatg attgaaaatc aaaatttgaa
    16381 tacacggaga ataccagaag gcctggaaaa acgcaagttt tacggcatct ctcgcgaagc
    16441 atatactata gcaaatgatt agccatctta gaggagtggt cgagcagaag ggcctcaatt
    16501 tcgccgtgct cgacgtgcag ggcgtgggct acagggtttt catgacctcg ggcctgttgg
    16561 ccgaacttgc cattggcgag gaagcgaaga ttccgaccta tctcgcagtc cgcgaagacg
    16621 ccatggatct ctacggcttc cgcaatcagg acgagctccg cttcttcgag cttctcatct
    16681 ccatttccgg catcggaccg aaatcagccc tgtcgatcct caatattacg acgcttgatt
    16741 ccctccgaca ggccgttgcg tccgaagaca ccacccacct cacccgagtt tcgggcatcg
    16801 gccgcaagac ggctgagcgc atcgtcatcg agctccgcgg caagctcgac ggcttcgagt
    16861 tcgaggcgat cgcgactgac acgggaagca tcaaggaaga agtcgacgcg ctcgaagccc
    16921 tgaagtcgct cggctattcg cagaaggaat cgcaggaagc tttgaagctc gttccgaaag
    16981 acatcatcgg caccggcgaa aaggtgaagc acgccctgcg cctcctcgcg agcaatcaat
    17041 agcgcgcatt acgatgaatc cttcgaatat tcttgaaaaa acgctccgcc gggcgaagcg
    17101 cctcattccg aaatccctct tccgcgcggc ccagcctttc taccactacg cgctcgcgat
    17161 cttgggcgcg ctccgctacg gattcccgtc gaaaaaactc atggtcatcg gcgttaccgg
    17221 caccaaaggc aagtcgacga ccgtcgagct cgtaaactcg attctcgaag cggccggata
    17281 caagaccgcc gtcgccggca cgatccgctt caaaatcggg gacgattcgc ggcgcaatat
    17341 gtacaaaatg acgatgccgg ggcgcttctt tctccagagc ttcctcgcgc gcgccgtaaa
    17401 ggcgggatgc actcatgccg tgatcgagat gacgtccgaa ggctcgaagc aataccgcta
    17461 ccgcttcgtc tatcccgacg cgctcatctt caccaatctt tctcccgagc atatcgagtc
    17521 gcacggctcg tatgaaaagt atgttgaggc gaagctcgac atcgcacggc gagtgctccg
    17581 gagcggcaag ccgcgccgta tcctcgtcgc gaacggcgac gacaaggaaa cgccgaagtt
    17641 cctcgggctc ggaatccccg aatccctcac ctacttcctc tccgcgaccc cgcacgtatc
    17701 gagcgaatcc ggctcgacat tcacatttat gggcacagag atgcgcacgc ctcttcccgg
    17761 agaattcaac ctggcaaatg ctttgggcgc ggcaacgctc gcccaggcgc tcggtatcga
    17821 taccgccgcg atagccctcg gcatcaaaaa cctcgcgcat gttcccggcc gcgcggaaac
    17881 cgtgacgctc gcggaggatc acgagctcgc ccgcgagcag gacttcaccg tcgtggtcga
    17941 ctacgcccat acgcccgata gcctcaaggc aatctacggc gcgtacggcg ctcatcgaaa
    18001 aatctgcgta ctcggctcga ccggcggcgg ccgcgacacc tggaagcgcg gagcgatggg
    18061 cgcgatcgcc gacgaatact gcgacgagat catcctcacc gacgaagatc cgtacgacga
    18121 ggacccgcaa aaaattgtcg ctgacgtcgc ggaaggaatc aaaagcaaga agcccgatat
    18181 gctcatggat cggcgcgagg ccattcatgc tgcgctctcc cgcgcgcgga aaggcgacgc
    18241 cgtcctcatc accggcaaag gaaccgatcc ctacatcatg ggcaagaacg gcgcgaaaca
    18301 gccgtggagc gacgcaaata tcgcccgcga agagctggag aaggttctca aatctcgcgc
    18361 ttcacagcaa aaaagcgaac ctcaggaata gcaaaccttt cgatacatcg aaaggtctgc
    18421 tattctatgc ttatgcctca cgtttcaaag aaaaatatca gcaacaagat ctcattaaaa
    18481 attgaggaga gattgttgag agttgtaacc gggcttaatt caaaaaaatc agccatgtta
    18541 tttgagcttc tcaccattac tgagaaaact atgctcgcaa agcgtctggg tatcgtattt
    18601 atgcttactg aaaaacgttc cttttatgaa atatctgaga agctcggtgt tagcacctca
    18661 acggtttcta gaatcgaaga tgatatgaaa agaggcgctt ttaaacgctt gcgcatttac
    18721 tttgaaaaac atagggaggg attttggaat tctctcgaaa aattcatgcg ggctgggatg
    18781 ccgccgattg tcggaaaagg gagatgggac tacttaaaag aaatgccctg aacctttcga
    18841 tatatcgaaa ggttcaggtt ggtgtcaatt cggtttttca gatacgattc gaaacatacc
    18901 taatttatta aattccatgc cggaacttcc cgaggtgcag acgacggtaa acggagtgaa
    18961 gcgctacgcc aagggcaagc gcattgtgga cgtctggacc gactacggca gcccgttcca
    19021 cgaaggaaaa tcgaacatca agaacaggaa atatttcgcc cgtttcaaga aagaagtggc
    19081 ggggcggagc atcactggcg ccgagcgcat cggcaaaaat gtaatcattc acctctcggg
    19141 cggaaaatcg atcgtcgtac atatgaaaat gaccggccac cttctcttcg gcgaatacgc
    19201 cttcgacgga aaaacctgga cggcgaaaca tgcgggcccg cttcgcgacg acggcttcaa
    19261 ccgcttcatc catctcctct ttaccctctc cggcggcaag catctcgcgc tctctgacat
    19321 gcgcaaattc gcgcgcgtaa gcgtcgtgga aacctcttcg ctcccccatg ccgacgacct
    19381 cgactcgatc ggacccgatc cgttgaatag ggattttaca tacaaagttt tcaaagggcg
    19441 gctcttgaag cgcccggccg gaaagataaa gcaggcgctc atggagcaaa aactcattgc
    19501 gggcatcggc aacatctact ccgacgaaat tctctggcgc gcgggcgtcc atcccctctc
    19561 tat
//
LOCUS       DASZYS010000002        15207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216 k141_1828,
            whole genome shotgun sequence.
ACCESSION   DASZYS010000002 DASZYS010000000
VERSION     DASZYS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VHF05_00165"
     CDS             <1..258
                     /locus_tag="VHF05_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HVS79379.1"
                     /translation="MGQAETRAFNRFILAGAVEIEVDAMGRILVPDFLRDFADLKTKV
                     VFAGVHNRVEIWNDKRWAEYKERTVKQADMMAEKLGQIGLL"
     gene            268..1185
                     /gene="rsmH"
                     /locus_tag="VHF05_00170"
     CDS             268..1185
                     /gene="rsmH"
                     /locus_tag="VHF05_00170"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVS79380.1"
                     /translation="MIHKSVLLNEAIEGLDIREGDIFLDCTLGNGGHSLEVFKRFGTS
                     VKIVGLDADSDAIARSAERLASFGCEIIAVAANFRHVSAVLDGLDIPKVDRVLMDLGL
                     SSNQIEESGRGFTFQKDEPLLMTFSNELGDGSLTAQAIVNFWDEENLETILRAYGEER
                     FARRIARGIVAAREAGPIETTGELVEIVARSVPAVYRHGRIHFATRTFQALRMTVNDE
                     LQALAEGMEQAFERLNVGGRLAVISFHSGEDRIVKNFFRAKAAAGAGRLIAKKPLSAT
                     DEEIAENPRSRSAKLRIVEKIGEGEEGNY"
     gene            1209..1550
                     /locus_tag="VHF05_00175"
     CDS             1209..1550
                     /locus_tag="VHF05_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79381.1"
                     /translation="MTTLSKGIRRGRAQVLAITNEPAAYWLLAAILVISFALYVYFVT
                     DTVKNVVKRQNLESEISTLNSEIGDLEFKDISMKNDVTLDKALAMGFKEVETTKYITR
                     TAGQVAMNGSI"
     gene            1626..3335
                     /locus_tag="VHF05_00180"
     CDS             1626..3335
                     /locus_tag="VHF05_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVS79382.1"
                     /translation="MKHNYRTRIRFISIVTMLFSLVLIGRLYSIQIVSGSEFTARAEH
                     QYLNNDYSLYDRGTIYFTNKDGTLVPAATLKTGYTIAINPEQLTDATGTYEKLDALTP
                     VDKNLFMASASKADDPYEEVAKQVDPAVAQKIQGLDITGLNTYRQQWRFYPGEEMAAH
                     ALGFVGYQGNDFGGRTGLERYYEDVLNRNASDSYVNFFAEIFSNIKDNIESNDAHEGD
                     VITTIEPSVEAFLERELKNVNAMYHSQFTGGIIMNPQNGEIYAMAVDPTYDPNDVKDI
                     KSPSILTDKLVEDVYEMGSIVKPLTMAAALDVGAVTPQTTYDDTGCIVVNSSRICNYD
                     LRARGVIPMQQVLSQSLNVGAAWVEHQIGNQTFAKYMLSYGIGQKTGIDLPNEASGIV
                     NNLTSPRDVEYATASFGQGIAMSPIETIRALAVLGNGGHLVTPHLAKAIKYENGVTKT
                     LSYPTGPQVIKPETSKTITDMLINVFDIALANGKAKIPDMTVASKTGTAQIADPNGGG
                     YEPNQYLHSFFGYFPATNPKFIIFLYTFKPQGVEYASETLTNSFLNLTHFLINYYEIP
                     PDR"
     gene            3482..4786
                     /gene="murF"
                     /locus_tag="VHF05_00185"
     CDS             3482..4786
                     /gene="murF"
                     /locus_tag="VHF05_00185"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HVS79383.1"
                     /translation="MKPLFKKIVVRILTAEARAVLRRRSPKIVAITGSVGKTSTKDAI
                     FAVMSEKFFVRKSAKSFNSELGVPLTILGLQNAWSNPLLWLKNILEGAMAALGSGRYP
                     QWLVLEVGADHPGDIKNLTEWLRPDITVITRLPEIPVHVEFFPTPDAIKREKAYLAEA
                     LKRDGTLVLNFDDDDVMTVREYAPDNALVTYGFGAGADMLGSLMQIRYDEENSSKVPR
                     GTNFRVDYQGKSVPVHVYGSLGAQHVYPALAAMAVGASLGLNMIEMAESLGKHRGPQG
                     RMRILDGVKGSVLIDDTYNSSPVALHQALDTLQEIEVSGKKYAVLGDMLELGKYSIDE
                     HRKAGKHAAEVADVLLTVGVRARDIAAAAMDFGMAENNIFQYENSVDAGKELDRMLEK
                     GDIALIKGSQGIRMEKTVFEVIADPDHAKELLVRQDAEWENR"
     gene            5181..5254
                     /locus_tag="VHF05_00190"
     tRNA            5181..5254
                     /locus_tag="VHF05_00190"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5216..5218,aa:Glu,seq:ctc)
     gene            5494..6699
                     /locus_tag="VHF05_00195"
     CDS             5494..6699
                     /locus_tag="VHF05_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79384.1"
                     /translation="MSRTNRTKKAPGAGEQSASQKTEPLTEAGEGKRRAVIAGKILLA
                     FVFLFFLYACFYAEFFPFASKITVYNDAVAAKTEAPQPPPKPVLNKADYDKRMLALAN
                     NPPPPPVATSTATTSSSTPPAVTKPQLWPAKDNPYPNAGALLPFNRIVAYYGNFYSKQ
                     MGVLGEYPEDEVLAKLQATAQSWRDADPSTPVIPAIDYIAVTAQGSPGADGKYRVRMP
                     DSQIDHALEMADRVGGIVVLDVQVGLSSLETEVPLLEKYLKMPNVELAIDPEFAMHNG
                     SRPGTVIGSLDASDVNFVAEYLAKLVDENNLPPKVLVVHRFTQKMVTNAADIKPLPQV
                     QVVMDMDGWGGPQLKKNSYRLTIYNEPVQFAGFKLFYKNDLLPPSTGMMTPEEVLDLQ
                     PKPIFIQYQ"
     gene            7046..7351
                     /locus_tag="VHF05_00200"
     CDS             7046..7351
                     /locus_tag="VHF05_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79385.1"
                     /translation="MNIHIAYARGENPQEESPACRVALGLLEDEFVLTGIYLQLGLRE
                     RSEEGIRNFFKNAALRAGLTPAEAERFSQRFSVIEPEISFKVPPVDPVGPLVMSRTA"
     gene            7489..8028
                     /locus_tag="VHF05_00205"
     CDS             7489..8028
                     /locus_tag="VHF05_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79386.1"
                     /translation="MDSVLHWRYSPEWENKTPIQKSVPFLENIQEAIKEGERVLKQRD
                     RENVMEALVKVCRAFSQFQDNFDVKIFAYAANRSSGRSSDAALRKLAHDFMSAAAVFG
                     RTKQGINRSEPGQSVEATNIFLPREPFQTQEKSWQSVSYWEIAGSCVNWGAISAAKHY
                     LETAKQLLPDLARLIRTYK"
     gene            8100..8489
                     /gene="ytxJ"
                     /locus_tag="VHF05_00210"
     CDS             8100..8489
                     /gene="ytxJ"
                     /locus_tag="VHF05_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HVS79387.1"
                     /translation="MKQIYTPEEWEAALEESKSGPIVIYKHSTACAGCERQLSEMHEG
                     VAYKEITRPVYYVVVQESGELSKRIADDLQIPHQSPQAIIVINGRACYDAYADEISAS
                     VIAARIHLVQEAFDEISGSGSKTREEE"
     gene            complement(8524..9102)
                     /locus_tag="VHF05_00215"
     CDS             complement(8524..9102)
                     /locus_tag="VHF05_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79388.1"
                     /translation="MHTHNGIIIKIRPEGLQKMFIEMISDVLSEEYRSLPARQFCSLD
                     LVLPHVSLILARTIMKSADTESENHFEIHGLREIKNEYLKMFEAPVTPEKLQMLGNET
                     LVSLGLCYAGLKRAHYLAINRAFDPLKNISFFVKMGSSFLRTAATHLSGMENDETRFI
                     LNSLADNFKLWTEVISYIPVRSSELRIDINGN"
     gene            complement(9191..9418)
                     /locus_tag="VHF05_00220"
     CDS             complement(9191..9418)
                     /locus_tag="VHF05_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79389.1"
                     /translation="MPNLSFEEFQENEQAWKEFEQQDPCAPVHEQQPPDLDKQMRREA
                     VIRQHILNQISNGETPLEGLWHMPRPEKTFH"
     gene            9647..10870
                     /gene="tgt"
                     /locus_tag="VHF05_00225"
     CDS             9647..10870
                     /gene="tgt"
                     /locus_tag="VHF05_00225"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HVS79390.1"
                     /translation="MENPISFRIEGKLGGGRLGRAGVLSTPHGEIKTPAFVTVGTKAT
                     VKGITPEQLRTLGAQVVLANTYHLFLQPGDELVRDAGGLHTFMHWQGPMMTDSGGFQV
                     FSLGAAYGKEISKITKETDPQLLLNEGTPDPDSPKLAKIDADGVTFKSHINGDMHYIT
                     PEKSIEIQHNLGADMIFAFDECTSPLEPLHYQQEALDRTHRWAKRSLDYHKSKENSKR
                     QALFGIVQGGRHEELRKESAEKLAGMDFDGYGIGGSFAKEDMSTAVKWVNGILPEDKP
                     RHLLGIGEPLDLFMGAENGVDLFDCVAPTRLGRTGTVYTRNGRLSLLRASCVDDFSKI
                     DEDCDCYACMNYTRSYLAHLFRAKEMLGPLLGSIHNLRFTIRLVDRIRESILDGSFDA
                     LKADVLGAYYGSTEK"
     gene            11155..13191
                     /gene="uvrB"
                     /locus_tag="VHF05_00230"
     CDS             11155..13191
                     /gene="uvrB"
                     /locus_tag="VHF05_00230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HVS79391.1"
                     /translation="MSDFKLKSEFKPSGDQPEAIDGLVEGVEKGFRHQTLLGVTGSGK
                     TFTAANVIAKVGKPTLVIAHNKTLAAQLAQEYREFFPENAVHYFVSYYDYYQPEAYLP
                     VTDTYIEKEAQINEEIERLRHASTQALLTRKDVIIVASVSCIYGLGSPAEYEKVNMRI
                     SRGMALSRAEFVRKLIDIHYDRTTADLAPGLFRAIGNTVEIMPVSERAIYRLELEGGT
                     IGRIQKIDDITREVLDEVETFFLFPAKHFITADDQVERAFGTIKAELEDRLRELDKEG
                     KILEAERLRRRTKYDLALIREIGYCNGIENYSRHFSGKMPGEPPDTLLSYFPHKKDGS
                     SDFLTIIDESHVTIPQIGGMYAGDQSRKENLVEHGFRLPSAKDNRPLKFDEFMERVGQ
                     IVYTSATPGKYEKEHSERIVEQVIRPTGLTDPEVSVRPIVEKGEYKGQIFDFIHEAEK
                     VIEHGGRAIATTLTKLMAEDLSEYLKEKGIETRYLHSDVKTIERIEILTSFRRGEFDV
                     LVGVNLLREGLDLPEVEFIGILDADKEGFLRSETSLIQIIGRAARNVLGHVVLYADNV
                     TGSMERAISETNRRRAIQIAYNKKHGITPKTIIKKIHDITDQLQREHDKAVLSQLAID
                     QKLLETDPKKVIREKERAMADAVKELDFETAAILRDEIQELLKMTNGGKRAKKK"
     gene            13710..>15207
                     /gene="uvrA"
                     /locus_tag="VHF05_00235"
     CDS             13710..>15207
                     /gene="uvrA"
                     /locus_tag="VHF05_00235"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HVS79392.1"
                     /translation="MPSKPTKTDVGAGHDKIVVKGARTHNLKNITVEMPRNKMVVFTG
                     LSGSGKSSLAFDTIFAEGQRRYVESLSSYARQFLRQMQKPDVDEIAGLSPAISIDQKS
                     RSNNPRSTVATITEIYDYLRILYARVGHPHCLICGREITRLSNEEIKAHVLEKIGEKQ
                     KFVARRSVKIGLPAYVEIFSPVVRGRKGEYYQLLYNLLGKGYSEVLVDGKRFRLRERI
                     DLDKNKRHDIAVLVDSINVADFASDPKGSDERLSEDLEKALEESEGLVMIRFRDLAKE
                     NTDTSAEEFTMSSKFSCPDDGYSYPEIEPRLFSFNSPYGACPECNGLGTKYLFGNQPC
                     PKCNGARLREEALHILVGQKNIVDLTAMSIEDADAFFDSLPLTPKEKEISKVVIREVV
                     SRLEFMKNVGIEYLTLDRRAHTLSGGESQRIRLASQLGSGLVGALYVLDEPTIGLHAR
                     DNDRLIKTLLDLRDLGNTILIVEHDEDTIYASDYIVDIGPGAGVHGGNV"
BASE COUNT         3737 a         4095 c         4065 g         3310 t
ORIGIN      
        1 atggggcagg cggagacgag ggcgttcaac cgcttcattc tcgccggcgc agtcgaaatc
       61 gaggtggacg ccatggggcg cattctcgtt cccgattttt tgcgcgattt cgcggacctc
      121 aagacgaagg tcgttttcgc gggcgtacac aaccgcgtgg aaatatggaa cgacaagagg
      181 tgggcggaat acaaggagag gacggtgaag caggccgata tgatggctga gaagctcggc
      241 cagatcggac tactttaatc gccgtttatg atccataaat cggttctttt aaacgaagca
      301 atagaaggtc tcgacatccg cgagggcgac atcttcctcg actgcacgct gggaaacggc
      361 ggccacagct tggaagtatt taagcgcttc ggcacctcag tgaagatcgt gggactcgac
      421 gccgacagcg atgccatagc gcggtcggcg gagagattgg cgtcattcgg ctgtgaaatc
      481 atagctgtcg ccgcgaactt ccggcacgta agcgcagtgc tcgacggact cgatattccg
      541 aaagtcgaca gggttctcat ggacctcgga ctttcctcga atcagattga agaatcgggg
      601 cgcggtttca cctttcagaa ggacgagccg ctcctcatga cattctcgaa cgaactgggc
      661 gatggaagcc tgacggctca ggcaatcgtc aacttttggg acgaggagaa tctcgaaacg
      721 attctccggg catacggcga agagcggttc gcgaggcgga tcgccagggg aatcgtcgcg
      781 gcgcgcgaag ccgggccgat cgagaccacc ggcgagcttg tcgagatcgt cgcgcgctcg
      841 gtgcctgcgg tgtaccggca cggtcgcatc cactttgcga caagaacctt ccaagccctc
      901 cgcatgacgg tgaacgacga actgcaggca cttgccgaag ggatggagca ggcgttcgag
      961 aggctcaacg tcggcggaag gcttgcggtg atctcgttcc atagcggcga ggaccgcatc
     1021 gtgaagaatt tcttccgcgc gaaggctgcc gccggcgcgg gtaggctcat tgcgaagaag
     1081 cctctgtccg caaccgatga ggagattgcg gaaaatccgc gttcgagaag cgcgaaattg
     1141 cggatcgtag agaaaatcgg agagggagag gagggaaatt attaaaagct aagcagtaca
     1201 ttcattttat gacaacgtta tccaaaggca tacggcgggg aagggcgcag gtccttgcca
     1261 tcaccaatga gccggccgcg tactggctct tggccgccat cctcgtcatc tccttcgctt
     1321 tgtatgtgta tttcgtcacc gatacggtga agaacgtcgt aaagcgccag aatctcgaga
     1381 gcgagatctc cacgctcaat tccgaaatcg gcgacctcga gttcaaggat atttcaatga
     1441 agaatgacgt taccctcgac aaggcccttg cgatgggctt caaggaggtg gaaacgacca
     1501 aatacatcac tcgcaccgcc ggccaggtgg caatgaacgg ctccatttaa tatagtgcgg
     1561 gctcatgtgt tgcccggccg cctcggcatg ctactatttg agggttttag ggctttccgc
     1621 atcagatgaa gcataattac cgcacacgca tcaggttcat atcgatcgtt accatgctgt
     1681 tctccctcgt tcttatcggg agactgtatt cgatacagat cgtttcggga agcgagttta
     1741 ccgcccgcgc ggagcaccaa tatttgaata acgattacag cctctacgac aggggcacta
     1801 tctatttcac caacaaggac ggcacgctcg tgcccgccgc gacgctcaag accggctaca
     1861 cgatagcgat aaatcccgaa cagctcactg atgccacggg gacgtatgag aagctcgatg
     1921 cgctcacgcc cgtcgacaag aaccttttca tggcgagcgc atcgaaggcg gacgatccgt
     1981 acgaggaagt ggcgaagcag gtcgacccgg cggtcgccca gaagatccaa gggctcgaca
     2041 tcacgggcct caatacgtat cgccagcagt ggcgcttcta cccgggcgaa gaaatggcgg
     2101 cgcacgcact gggcttcgtc ggctatcagg gcaacgactt cggcggccgc accggcctcg
     2161 agcgctacta cgaggacgtg ctcaatcgca acgcgagcga ttcgtacgtc aatttcttcg
     2221 cggaaatatt ctccaacatc aaggacaata tcgagtcaaa tgacgcgcac gaaggcgacg
     2281 tcatcacgac catcgagcct tcggtcgagg ctttcctcga gcgcgaactc aagaacgtca
     2341 atgccatgta ccactcgcaa ttcaccggcg gcatcatcat gaatccgcag aacggcgaaa
     2401 tctacgccat ggccgtcgat ccgacgtacg atccgaacga tgtgaaggat ataaagagcc
     2461 cgagtatcct gacggacaag ctcgtggagg acgtgtatga aatgggttcg atcgtgaagc
     2521 cgcttaccat ggcggcggcg ctcgatgtcg gggcggttac tccccagacg acatacgacg
     2581 ataccggctg catcgtcgtc aattcctcgc gcatctgcaa ttacgacctg cgcgcccgcg
     2641 gcgtgattcc catgcagcag gttttgagcc aatcgctcaa tgtcggcgcg gcatgggtcg
     2701 agcaccagat aggcaatcag acgttcgcga aatatatgct tagctacggc attggccaga
     2761 agaccggcat cgatctgccg aacgaagctt ccggcatcgt gaacaacctg acgagcccgc
     2821 gcgacgtcga atacgcgacc gcgtccttcg gccagggcat cgccatgtcg ccgatcgaga
     2881 cgatccgcgc gctcgcggtt ttgggcaacg gcggccatct cgtaacgccg cacctcgcga
     2941 aggcgatcaa atacgagaac ggcgtcacaa aaaccctctc gtacccaaca ggcccgcagg
     3001 taatcaaacc ggaaacctcc aagacgatca ccgacatgct catcaacgtg ttcgatatcg
     3061 ctctcgcgaa cggcaaggcg aagattcccg acatgactgt cgcttcgaag accggaacgg
     3121 cccagatcgc cgatccgaac gggggaggat atgagccgaa ccagtatctc cactcgttct
     3181 tcggctattt cccggccacg aatcccaagt tcatcatttt cctctataca ttcaagccgc
     3241 aaggcgtaga atacgcatcg gaaacgctca cgaattcgtt cctgaacctc acgcatttcc
     3301 tgatcaatta ttacgaaata ccgcctgacc gctaaagcgg aatttccaat ttacaattat
     3361 caattttcaa tcaaatcaca atgaatcaat tcctgcctac aggcaggcca ggcaggttca
     3421 aacgtttggg acattgaaat attgaaaatt cattgaaaat tagaaattga aaattaatat
     3481 agtgaaacct ttatttaaaa aaatagttgt tcgaattctc accgccgaag cgcgagccgt
     3541 ccttcgcagg cgttctccca agatcgtcgc cataaccggc agcgtgggaa agacgtcaac
     3601 caaggacgca atcttcgccg tgatgtcgga aaaattcttc gttcgcaaga gtgccaagag
     3661 cttcaatagc gaactcggcg tccctctcac catcttgggc ctccagaacg cctggagcaa
     3721 tcctcttttg tggctcaaaa atattttgga aggcgccatg gcggcgctgg gctccggccg
     3781 ctatccgcaa tggctggtgc tcgaagtcgg cgccgaccat cccggcgata tcaagaacct
     3841 tactgaatgg ctccgacccg atatcacagt catcaccagg cttccggaaa ttccagtgca
     3901 tgtcgaattt tttcccacac ccgatgccat caagcgcgag aaggcgtatc tcgccgaagc
     3961 gttgaagcgc gatggcacgc tcgtcctcaa tttcgatgac gacgacgtca tgacggttcg
     4021 cgaatatgct cccgacaatg ctcttgtaac ctatggattc ggcgcgggcg ccgacatgct
     4081 cggctccctg atgcagatcc gctacgacga ggagaacagc tccaaagttc cgcgcggcac
     4141 gaatttccgc gtggactacc agggcaagag cgtaccggtc catgtctacg gatcgctcgg
     4201 cgcccagcat gtctatcccg cccttgccgc tatggcggtg ggcgcgagtc tcggcctcaa
     4261 tatgatcgaa atggcggaat ctctcggcaa gcaccgcggc ccgcagggaa ggatgcgcat
     4321 tctggatggc gtcaaaggat ccgtccttat cgacgatacg tacaactctt cgccagtcgc
     4381 gcttcaccaa gcgctcgata cgctccagga aatcgaagtg agcggaaaga aatatgccgt
     4441 tttaggcgac atgctcgagc tcggaaaata ttcgatcgac gagcaccgga aagcgggcaa
     4501 gcatgcggcg gaagtcgccg acgtcctcct cacagtaggc gtccgcgctc gcgacatcgc
     4561 cgcggcagcg atggatttcg gcatggcgga aaacaatatc tttcaatatg aaaactctgt
     4621 cgatgccggc aaggagctcg atcggatgct cgagaaaggc gacatcgcgc ttatcaaagg
     4681 ctcgcaggga atccgcatgg agaagaccgt gttcgaagtg atagccgatc ccgatcatgc
     4741 gaaggagctt ctggtgcgcc aagatgcgga gtgggaaaac cgataatttt gacaaagact
     4801 ttatttcatt ctccaaccat caaacgcact tgcatttgtt gcgagtggag attactatac
     4861 tagtgtaagt ttaatactta cattgttcct ttgaagattg agtttcaatc ttcaccaacc
     4921 aaagtttcga gattctcaaa tctcaaccaa ctttcctgaa gttttaaaaa aacttctcaa
     4981 agtttccgag atttcacaaa tctcaaacca atccgttttt cgaaattttt ctcaaaaatt
     5041 tcacccaacg cactctgcat cacttatgtg aacgcagggt ttttgtttat atactttgta
     5101 attttgcata aatctgctac tatctttttt gtccttaatc aattttgctt tttgcatttt
     5161 gatttttgat ttaatttatt ggccctatcg tctagcccgg ttaggacaga tccctctcac
     5221 ggatcaaact ggggttcgaa tccccatagg gtcacaaagt gccaaccgca aagcgaaagc
     5281 tctgcggttg ttgcatcttt gcagacccta tggggattcg aaaggaaatt cgggacccga
     5341 tttagcaaaa tcgggtgaat ttccagggcc gagaggagaa agttttgacg aaaactttcg
     5401 agctcttggc cgaatcccca tagggtcgga taattatccc catatccacg cctataattc
     5461 gcctcatttc cgcgtttact ataaaatagt cgtgtgagtc ggacgaatcg gacaaaaaag
     5521 gctcccggag ccggtgagca gagcgcatct caaaaaacag agcctcttac ggaggcgggc
     5581 gagggaaagc ggagggctgt catcgcaggc aagattcttc ttgcgtttgt ttttttattt
     5641 tttctctatg catgttttta tgccgaattt ttcccgttcg cctcgaaaat aaccgtctac
     5701 aacgacgcag tggcggcgaa aaccgaggcg ccccagcctc ctccgaagcc cgttctcaat
     5761 aaggctgact acgataagcg gatgcttgcg cttgcgaaca atccgcctcc tcctcctgtt
     5821 gccacctcca cggctacgac ctcctcaagc actcctcccg ccgttacgaa gccccagttg
     5881 tggcctgcca aagacaaccc gtatccgaac gcgggcgcgc ttctgccgtt caaccgcatc
     5941 gtggcctatt acggcaattt ctattcgaag cagatgggcg tgctcggcga atatccggag
     6001 gatgaagtgc ttgccaagct ccaggcgacc gcgcagtcgt ggcgcgatgc cgatccgtcg
     6061 acgccggtca ttcccgccat cgactacatc gccgtgacgg cgcagggaag cccaggggcg
     6121 gacgggaaat atcgcgtgcg catgcccgac agccagatcg atcatgctct tgagatggcg
     6181 gacagggtcg gcggcatcgt cgttctcgac gtgcaggtgg gcctgagcag cctagaaacg
     6241 gaggtgccgc ttctggagaa atatctcaag atgccgaatg tcgaactcgc gatcgacccc
     6301 gaatttgcaa tgcacaacgg ctcacggccc ggcacggtga tcggaagcct cgatgcgagc
     6361 gatgtgaatt tcgtcgccga gtatctcgcg aagcttgtcg acgaaaacaa tctcccgccg
     6421 aaagttctcg tcgtccatcg gtttacccaa aaaatggtga caaacgcggc ggatataaag
     6481 ccgttgccgc aagtccaggt cgtcatggat atggacgggt ggggagggcc gcagctcaag
     6541 aaaaattcat accggctcac gatctacaac gagcccgtcc aattcgccgg cttcaagctt
     6601 ttctacaaga atgatctcct gccgccctca acgggcatga tgactccgga agaggtgctc
     6661 gacctccagc ccaagccgat attcatccag tatcagtagc aaacacaata tcaaaaacgg
     6721 acgacgctta tgcgtcgtcc gtgtttccat aagcgtaata cttatggggc tgtatcccct
     6781 gaactgccag ggcgggtaat tattgcgtga tttgaggcgc acataaatgc gtgagcctct
     6841 gacaatcggc ctctcggcca gcaggtggcc gtggagtttc caaaaagaat cagctacatt
     6901 tcgctgtcgg cggctcaagc gcccgacgtt tttcatgatc gcgccctccg attgcatatt
     6961 agcacattaa ataattatgt caattagctc ttcatttcac ctttccttca gatgagcttg
     7021 ggaatgtttg tttggagagc acattatgaa cattcatatc gcatatgcaa gaggagaaaa
     7081 tcctcaggaa gaatcgcctg catgccgtgt ggcgctcggg cttctcgagg atgagttcgt
     7141 cctcactggc atctatttgc aactcgggct tcgtgaaagg agcgaagagg ggatccggaa
     7201 tttctttaag aacgccgcat taagggcagg cctgacgccc gcagaagccg agaggttttc
     7261 gcagaggttc agtgtaatcg agccggagat atccttcaag gtgcctccgg ttgatccggt
     7321 gggtcctctt gtgatgtcgc ggaccgcata acatatttgc agccgttcat tcattgagcg
     7381 gctgcatttt tataaaaatt cacaattatc ccgcaggtat tgactttact atatatttgt
     7441 tctattttaa ttacggagga tacgagcttg cgaacaattt cttcgatatt ggattccgta
     7501 ttacattggc gatatagccc cgaatgggag aataaaacgc cgattcaaaa atctgtgcct
     7561 ttcctggaga atatccagga ggcaatcaaa gaaggagagc gggtgctgaa gcagagagac
     7621 agagagaatg taatggaggc tctcgtcaag gtctgcaggg cattctcgca attccaggat
     7681 aattttgacg ttaaaatttt tgcttacgcg gcaaacagat cgtcaggcag atcgtcagat
     7741 gccgcattgc gaaagcttgc gcatgatttc atgtcggccg cagcggtttt cggccggaca
     7801 aagcagggta ttaatcgttc agaacccggt cagtcggtcg aggcaacgaa catttttctt
     7861 cctcgagaac cattccagac gcaggagaaa agttggcagt ccgtttctta ctgggaaatt
     7921 gcaggatcat gcgtaaattg gggagcaatt agcgctgcga aacattatct ggagacagca
     7981 aagcagctac tgccggatct tgctcggctc atccgaacat ataaataatc gttctttcat
     8041 ccgctcgggc ttcggctcga gcggttcttt tattaagggc gctcggatat aattgtcgta
     8101 tgaagcagat ctacacgccc gaggaatggg aggcggcgct cgaggaatcg aagagcggcc
     8161 ccatagtcat ttacaagcac agcaccgcgt gcgccggatg cgagcggcaa ctgagcgaga
     8221 tgcacgaggg cgttgcatac aaggaaatca cccggccggt ctactatgtg gtggtgcagg
     8281 aatcgggcga gctttcgaaa aggatcgccg acgacctcca gatcccgcat caaagtccgc
     8341 aggcgatcat cgtgataaac gggcgggcat gctacgacgc ttacgcggac gagatcagcg
     8401 cttctgtcat cgccgcgcga attcatctcg tccaggaagc cttcgacgag atcagtggct
     8461 cgggttcgaa aacaagggag gaggaataaa aaacgcgccg cacattcgtg cggcgcgcgt
     8521 tttttagttg ccattgatat caatcctcaa ttcactgctt cttacgggaa tgtatgaaat
     8581 cacctcggtc catagcttaa agttgtcggc tagggaattg agaatgaatc gtgtctcatc
     8641 attttccatg ccgctcaagt gggttgcggc ggtgcgcaga aagctgctgc ccatttttac
     8701 aaaaaagctt atatttttga gcgggtcaaa agctctattg atggccaggt aatgagcacg
     8761 cttgagtcct gcataacaca accctaatga aacgagcgtt tcgttgccga gcatttgaag
     8821 cttctcaggc gtgacgggag cttcaaacat tttaagatat tcatttttaa tctcccttaa
     8881 tccatggatt tcaaaatgat tctctgattc ggtgtcggca gatttcatta ttgtgcgggc
     8941 aaggataagc gatacatgcg gcaggacaag atccaggctg caaaattgcc gggcgggcaa
     9001 tgatcgatat tcttcagaga gaacatccga gatcatttca atgaacatct tctgcaagcc
     9061 ttcgggtctt attttgatga taatcccatt atgcgtatgc atttggaacc tcctaattcc
     9121 agattaggct ccttttactg ttgagtcaaa tgaaaagagc ccggacgcgt agcgccgggc
     9181 tcggaactga tcaatggaat gttttctctg gccgtggcat atgccacagg ccttctaacg
     9241 gcgtttcgcc gttagagatt tggttcagga tatgttgccg gattaccgct tcacgtctca
     9301 tctgcttgtc gagatcgggc ggctgctgtt cgtgtacagg agcgcaagga tcctgctgct
     9361 cgaattcctt ccacgcttgt tcgttttctt ggaactcttc aaagctcaaa tttggcatat
     9421 catgcctcct attaatatac tacaattcat atcaaatcag gtcaacagta gggaaaattg
     9481 ccctcaattg cttgagacta tatagttagc acgatggtcc cgttgtagaa aatggcattt
     9541 gaaagaaagt tagaaatgtg tggacgtata aaaaattaat tgttagctta aggttaattt
     9601 taaagatggc ctgctccgaa ggagcgatgc catttctcac aacaggatgg aaaatccgat
     9661 ttcattcagg atagagggaa agctcggcgg cggacggctc ggccgcgcag gcgttttgtc
     9721 gacgccccat ggcgagatca aaactcccgc tttcgttacg gtgggcacca aagccactgt
     9781 gaagggaatc actcccgaac agttgagaac gctcggcgcg caagtcgtgc ttgcgaacac
     9841 gtaccacctt ttccttcagc cgggggacga gctggtgcgc gatgcgggcg gcctgcatac
     9901 gttcatgcat tggcaggggc cgatgatgac tgattcaggc ggcttccagg tgttttcgct
     9961 cggagcggcg tacggcaagg agatcagcaa gatcaccaag gaaaccgatc ctcagctgct
    10021 tttaaacgaa gggacgccgg atccggacag tccgaaactt gcgaagatcg acgccgacgg
    10081 tgtgacattt aaatcgcata taaatggcga catgcactat atcactcccg agaagtcgat
    10141 tgagatccag cacaatctcg gcgcggacat gattttcgcc ttcgacgagt gcacgtcgcc
    10201 cctggagccg cttcattacc agcaggaagc gctcgaccgc acgcaccgct gggcgaagcg
    10261 aagcctcgac tatcacaagt cgaaggagaa ttcgaagcgg caggcgcttt tcggcatcgt
    10321 gcagggcggc aggcacgaag aactccgcaa ggagagcgcc gaaaaattgg cggggatgga
    10381 tttcgacggc tacggcatcg gcggctcatt tgccaaggaa gacatgtcga cggcggtgaa
    10441 atgggtgaac ggtattctgc ccgaagacaa gccgcgccat ctcctcggca tcggcgaacc
    10501 gctcgatctc ttcatgggcg cggaaaacgg cgtggatctt ttcgactgcg tggcgccgac
    10561 gcggctcgga cgcaccggca ccgtctacac tcgcaacggc cggctgagcc ttctcagggc
    10621 gagctgtgtc gatgatttct cgaagatcga cgaagactgc gactgctatg cttgcatgaa
    10681 ctacacgcgg agctacttag ctcatctttt ccgcgccaaa gaaatgctcg ggccgcttct
    10741 cggatcgatt cataatcttc gctttaccat acggctcgtc gatcgcatca gggagagcat
    10801 tctcgacggg tcgttcgacg cgcttaaggc ggacgtgctc ggcgcgtatt acggatcaac
    10861 tgaaaaatag attgccactt gccgcgaccg cggaaaatgg caatctgccc cgagcagata
    10921 aaaaagtgaa aacgggagcg cgcgaagctc ccgtccagga ggtgtaaaac atgagcttgt
    10981 cacctctttt ctcgggggag aagagagacg agttccattg tctcgaagca ttcaatcatc
    11041 cgaatataag aaaacgatga agcgcaaaaa ttcgtttttt tgattttccg cgctaggcgg
    11101 gtatagttat atgcactaag gctgcaacgg gtcgaccttt cttttgcact atatatgagc
    11161 gacttcaaat taaaatcaga gtttaagccg tccggcgacc agcctgaggc gatcgacggc
    11221 ctcgtggaag gagtggaaaa aggcttccgc caccagacgc ttttaggcgt tacgggctcc
    11281 ggcaagacct tcacagccgc gaacgtgatc gcgaaagtcg gcaagccgac gctcgttatc
    11341 gcgcacaata agacgctcgc ggcccagctc gcgcaggaat atcgggaatt ctttcccgag
    11401 aacgccgtgc attatttcgt ctcctactac gactactacc agccggaggc gtatctcccc
    11461 gtcaccgata cctatatcga aaaggaagcg cagatcaacg aggaaatcga gcggctccgc
    11521 catgcgtcga cccaggccct tcttacccgc aaggacgtca tcatcgtcgc gtccgtttcc
    11581 tgcatctacg gcctcggcag tcccgccgaa tacgaaaagg tcaacatgcg catctcccgc
    11641 ggcatggcgc tttcccgcgc ggagttcgtg agaaagctta tcgacatcca ctacgaccgc
    11701 acgaccgcag acctggcgcc cggccttttc cgcgccatcg gcaatacggt ggagatcatg
    11761 ccggtgtccg agcgcgccat ctaccggctc gaactcgagg gagggacgat cggcagaatt
    11821 cagaagatcg acgatatcac ccgcgaagtg ctcgacgagg tggagacgtt ctttcttttt
    11881 cctgcgaagc acttcatcac tgcagacgat caggtcgagc gcgcgttcgg cacgatcaaa
    11941 gccgagctcg aggacaggct cagagaactc gacaaggaag ggaagatcct cgaagccgag
    12001 cgcttgcgcc gccgcaccaa gtacgacctc gcccttatcc gcgaaatcgg ctattgcaat
    12061 ggcattgaaa actactcgcg ccacttcagc ggcaagatgc ccggcgagcc gcccgatacg
    12121 ctgttgagct acttcccgca caagaaagac ggaagctcgg atttcctgac gattatcgac
    12181 gagtcgcacg ttaccattcc gcagatcggg ggcatgtatg cgggcgacca gtcgcgcaag
    12241 gaaaatctcg tcgagcacgg cttcagactg ccctccgcga aggacaacag gcccttgaag
    12301 ttcgacgaat tcatggagcg cgtcgggcag atcgtctata cctccgcgac gcctggaaaa
    12361 tatgagaagg agcatagcga gcgcatcgtc gagcaggtga tccgcccgac cggccttacc
    12421 gatccggaag tttccgtgcg ccccatcgtc gagaaggggg aatacaaggg ccagattttc
    12481 gatttcatac acgaagcgga aaaggtgatc gagcacggcg gccgcgccat cgcgacgacg
    12541 cttaccaagc tgatggccga agatctttcc gaatacctca aggagaaggg aatcgagacg
    12601 cgctacctcc acagcgacgt gaagacgatc gagcgtatcg aaatcctcac gtcgttccgc
    12661 aggggagagt ttgacgtgct tgtcggcgtc aatctcctcc gcgaaggcct cgaccttccc
    12721 gaagtggaat tcatcggcat tctcgatgcc gacaaggaag ggttcctgcg ctccgaaacg
    12781 tcgcttatcc agatcatcgg ccgcgccgcg agaaacgtct tagggcacgt cgtgctctat
    12841 gccgacaacg tgacgggctc gatggagcgc gccataagcg agacgaaccg ccgccgcgcg
    12901 atccagatcg cctacaacaa gaagcatggc atcacgccga agacgatcat caagaagatc
    12961 cacgacatta ccgaccagct ccagcgcgag cacgacaagg cggttctttc ccagctcgcg
    13021 atcgaccaaa agcttctgga aaccgatccg aaaaaggtga tccgcgagaa agagcgcgcg
    13081 atggccgacg cggtgaagga gctcgacttc gagacggccg caattctccg agacgaaata
    13141 caggagcttc tcaaaatgac gaacggcggc aaacgtgcta aaaagaagta ggaaaggggg
    13201 aactggatga gaacaggggg ttaacggtct tacttccgtg tcccaaacta acggttacgc
    13261 atcactacca aaaatgccgg cgcagtggag ctgcgccggt tttcatattg ttgccaaatg
    13321 acaaataata ccgggcatgc tacaaaaggg taagacctgt ttatagcgtc ggaaaggagg
    13381 cagcaactta tctattagtt gaactgaaat agcagtgctg agtaaaaatc cggcgcggga
    13441 aaccgcgccg atttttttat ttgaattgca gagctttgcg aatctcgtca agtagggtat
    13501 catacatgga caactgaata gccgcatatg caccctagga gtaagtccgg ggtaagtttc
    13561 ggctatggaa aataattttt taattttaaa tttgcaattt tcaatgaatt ttcaaagcat
    13621 caatgtcaca aagttcgtat tgtttggaaa ttaatcaatt ggatattgat tgaaaattgt
    13681 aaattgcaaa ttgaaaattt ttcattatca tgccttccaa accaacaaaa accgatgtag
    13741 gagccggaca cgacaagatt gtcgtgaagg gagctagaac gcacaatctc aagaacatta
    13801 cggtcgaaat gccgcgcaat aaaatggtgg tgtttacagg tctttccggt tcgggcaaga
    13861 gctcgctcgc gttcgatacc atctttgccg aaggtcagcg gcgctatgtc gagtcgcttt
    13921 cgtcgtatgc ccgccagttc ctccgccaga tgcaaaagcc ggatgtcgac gagatcgcag
    13981 gcctttctcc ggccatctcg atcgaccaga aatcgcgctc caacaacccg cgttcgactg
    14041 tcgcgaccat taccgaaatc tacgactatc ttcgcatcct ctacgcgcgc gtcggccatc
    14101 cacactgcct catctgcggc agggaaatca cccgcctctc gaacgaggag atcaaggcgc
    14161 acgtgcttga gaagatcggc gagaagcaga agttcgtcgc gcggcgttcg gtgaagatcg
    14221 gccttcccgc atacgtggaa atcttttctc ccgtggtgcg cggccgcaag ggcgaatatt
    14281 accagctcct ctacaatctt ctcggcaaag gctattcgga agtgctcgtc gacgggaagc
    14341 gctttaggct ccgcgagcgc atcgatctcg acaagaacaa gcggcacgat atcgcggtgc
    14401 tcgtcgattc gatcaatgtc gccgatttcg catccgaccc gaaaggctcc gatgagaggc
    14461 tttcggaaga ccttgagaaa gcgctcgagg aatcggaggg gctcgtgatg atccgcttcc
    14521 gcgacctcgc caaggaaaat accgatacgt cggcggagga attcacgatg tcctcgaaat
    14581 tctcctgccc tgacgacggc tactcgtatc cggaaatcga gccgcgcctt ttctccttca
    14641 actctcctta cggcgcatgc cccgaatgca acggcctcgg cacgaaatat ctctttggca
    14701 accagccctg cccgaagtgc aatggcgcga gactgcgcga ggaagcgctt cacatcctcg
    14761 tcggccaaaa aaatatcgtc gatcttaccg cgatgtcgat cgaggacgcc gacgcgttct
    14821 tcgattcgct tccgctcacg ccgaaggaga aggagatctc gaaggtggtg atccgcgagg
    14881 tcgtctcccg cctcgaattc atgaaaaatg tcggcatcga gtatctcact ctcgatcgcc
    14941 gcgcccacac gcttagtggc ggcgagtccc agcgcatccg gcttgcatcc cagcttggct
    15001 cgggactcgt cggcgcgctc tacgtgctcg acgagccgac catcggcctc catgcccgcg
    15061 acaacgaccg gcttatcaag acccttcttg acctgcgcga tctcggcaat acgatcctca
    15121 tcgtcgagca tgatgaggac acgatctacg cgtccgacta tattgtcgat atcggcccgg
    15181 gcgcgggagt tcatggcggc aatgtcg
//
LOCUS       DASZYS010000003         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_325527, whole genome shotgun sequence.
ACCESSION   DASZYS010000003 DASZYS010000000
VERSION     DASZYS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325527"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VHF05_00240"
     CDS             complement(<1..667)
                     /locus_tag="VHF05_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HVS79393.1"
                     /translation="MKPFSIKESLKKGWELVKAHFGLLIGATLVYLAFSSSGRALAGN
                     NGHIVGWSIPLFLILIVISIIIHIGYYKLYLKLVKGERVSISELFTNYSLFWRYLGGN
                     ILAGLVTMVGFILLIVPGIYFAVKYSFTSLLVIDQDLGPVEAFKKSAELTKGIKWYLL
                     LFWLTLIGINIVGVIVFVVGLLVTIPLSYAAYIYVYRAVIDAKGALPAEEVPMSPAPA
                     DPTQ"
     gene            complement(796..1383)
                     /locus_tag="VHF05_00245"
     CDS             complement(796..1383)
                     /locus_tag="VHF05_00245"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HVS79394.1"
                     /translation="MKTKTIWIVIVVVIAAAVIIYLLTGGAVTAPTTEDTNGEATTSQ
                     GAPLPQEGTSTPVGASSVNVYLVSVGTGEQGGENTVGCGDTIAAVKEDIPQTQGVLVA
                     ALDKLFSIKTQQVAVGSTTYYNALYQSDLSVDSAEVVNGVATVKISGKTALGGECDDP
                     RAIAQIRQTVMQFGTVKSADIFINDKPLEDYFSLK"
     gene            complement(1460..2371)
                     /gene="secF"
                     /locus_tag="VHF05_00250"
     CDS             complement(1460..2371)
                     /gene="secF"
                     /locus_tag="VHF05_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVS79395.1"
                     /translation="MFVINHRKVFYWISGTLMVLAVLAIIFIGFNPGIDFTGGSSVQV
                     TYEGARPDTASVRAALDASGFTSYELRPSGDNKYDLQTGELDQTKQNAAIKALSLDGQ
                     APLNLDSVNTIGPVVGRELANKAYIALALIILAIMLFIAFSFRQVSKPVSSWKYGIAT
                     IVALFHDVLIPTGIYVVYSKYTGAQIDLLFVTAVLFMLGYSVYDTIVVFDRVRENLKH
                     NHDSRHTEEFGHTVGKSLAQTYARSINTSLTLVLVLLALLVFGSPATHNFVFVLLMGA
                     IVGTYSSIFLASPMLVTMAEFSARKAK"
     gene            complement(2420..3787)
                     /gene="secD"
                     /locus_tag="VHF05_00255"
     CDS             complement(2420..3787)
                     /gene="secD"
                     /locus_tag="VHF05_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVS79396.1"
                     /translation="MIKHRIFSVFLVALVILLGFFLYASEHPGNAFSGFPFRYGLDLN
                     GGTELTYNADVSKLQSGDVSGAMSTLRDVIERRVNAFGVSEASVRVEDAGIISGNSEH
                     RLVVDLPGVTDTEKAIGIIGQTPSLEFKLLPYTDQELADKLGTSTLADHPELFQPTGL
                     TGGLLQHAQITFSSGGGTQLANQPTVTLQFNDQGRQLFADLTKNNVGKVLGIFLDNEL
                     ISSPVINEPIPDGNAQISGNFTPDEAKTLVRNLNYGALPVPINLASTQVVGATLGTQA
                     KDLGFKAGIVGFILVVIFLLVLYRVPGFLAAAALLLYVGISLTLFKLFGITLTAAGIA
                     GFIISMGIAIDANVIIFERLKDELRSGKKLFQSIHEGFHRAWAPVRDASISSLITAVI
                     LYWLGNAAVKGFAVTFGIGTLVSIFTAVTVTRTFLYAVVTEQTKETPLLRKLFGGRKA
                     DNQTT"
BASE COUNT          925 a         1098 c         1222 g          833 t
ORIGIN      
        1 gttgtgtcgg atcggccggg gcgggagaca tgggaacctc ttccgcaggc agggcgccct
       61 tggcatcaat gacggcgcga tagacgtaaa tgtacgccgc atacgagagc ggaatcgtga
      121 cgagaagccc caccacgaag acgatcactc cgacaatgtt tatgccgatg agggtgagcc
      181 agaagaggag gaggtaccac ttgatgccct tggtaagctc ggcgctcttc ttgaacgcct
      241 cgaccggacc gaggtcctga tcgatgacga ggagcgaggt gaagctgtat ttcaccgcga
      301 agtaaatgcc ggggacgatg agaaggatga agcccaccat ggtgacaagg ccggccagga
      361 tatttccgcc gagatacctc cagaaaaggc tgtagttggt gaagagctcc gagatgctca
      421 cgcgctcgcc cttcacgagc ttcaggtaca gcttgtaata gccgatatgg atgatgatcg
      481 aaatgacgat gagaataagg aagagcggaa tggaccagcc gacgatgtgc ccgttattgc
      541 cggcaagcgc cctgccgctc gaagagaatg caagatatac gagcgtcgcg ccgataagaa
      601 ggccgaagtg cgcctttacg agctcccatc ctttcttaag agattccttg atcgaaaatg
      661 gcttcatccc gtttagaagc agatcgcgat ctctcgcttt ctgccataaa taattaattt
      721 aataagtttc gacgtatgac tctcaatgtt tattccgttg agcgatcgcg gcttctaacg
      781 ggcttcattg ttcaattact tgagtgaaaa ataatcctca agcggcttat cgttgataaa
      841 tatgtccgcc gacttcaccg tcccgaactg catgaccgtc tgcctgatct gcgcgatggc
      901 ccgcggatcg tcgcactcgc cgccaagcgc cgtcttcccc gaaatcttca ctgtcgccac
      961 gccgttcacc acttccgccg agtcaaccga aagatccgac tggtagagtg cgttatagta
     1021 cgtcgtactg ccgaccgcca cctgctgcgt cttgatcgag aagagcttgt cgagcgccgc
     1081 cacgaggacg ccctgcgtct gcgggatatc ctccttcacg gcggcgatcg tgtcgccgca
     1141 gccgaccgta ttttcccctc cctgctctcc cgtgccgaca gacacgagat agacattcac
     1201 ggaactcgct ccgacgggag tcgacgttcc ttcctgcggg agaggcgcac cttgggatgt
     1261 ggtagcctct ccattcgtgt cttctgtagt cggcgcggtc accgctcctc cggtaagaag
     1321 atagatgatg accgcagccg ctatgacgac gacgatgaca atccagattg tcttggtctt
     1381 catgattgtt ttgctactcc cctattatac ctccgttttt cggctcttcc ctttccgtat
     1441 caacaagcgg gagaacgcgc tacttcgcct tgcgggccga aaattccgcc atcgtcacga
     1501 gcatcggcga cgccaggaag attgacgaat acgttccgac aatcgctccc atgagaagca
     1561 cgaagacgaa gttgtgggtc gcggggctgc cgaaaacgag aagtgcgaga agcaccagca
     1621 cgagcgtgag cgacgtgttg atcgagcgcg cataggtctg cgcgaggctc ttgccgacgg
     1681 tatggccgaa ctcctcggta tgcctcgaat catgattgtg cttcaggttt tcgcggacgc
     1741 ggtcgaagac gacgatcgtg tcgtagaccg aatagccgag catgaagagc actgccgtca
     1801 cgaagaggag atcgatctgc gcgccggtgt acttcgagta gacgacgtag atgcccgtcg
     1861 ggataagaac gtcgtggaag agcgcgacaa tggtcgctat gccgtatttc caggaagata
     1921 ccggcttcga gacctggcgg aacgagaacg cgatgaagag catgatcgcg agaatgataa
     1981 gcgcgagggc gatgtacgcc ttgttcgcaa gctcgcggcc gacgaccgga ccgatcgtat
     2041 tgacgctgtc gagattgagc ggagcctggc cgtcgagcga gagggccttt atggccgcgt
     2101 tctgcttcgt ctggtcgagc tcgccagtct gaagatcgta tttattatct cccgacggac
     2161 ggagctcgta cgacgtgaag cccgaggcgt cgagcgccgc acgcactgac gccgtatccg
     2221 ggcgcgcgcc ctcataggtc acctggacgc tcgagcctcc cgtgaagtcg atgccaggat
     2281 tgaagccgat gaagatgata gcgaggaccg cgagcaccat gagcgtgccg gagatccagt
     2341 agaatacctt gcgatggttg atgacaaaca tgttagtaat tcaaaattta aaaatcaaaa
     2401 gtcaaaagta aaatcgggat catgttgtct gattgtcggc tttcctgccc ccgaagagct
     2461 tccgcaggag cggcgtctcc ttggtctgtt cggtaacgac cgcgtagagg aacgtgcggg
     2521 tcacggtgac ggcggtgaag atcgaaacga gggtgccgat gccgaaggtg acggcgaagc
     2581 ccttgaccgc cgcgttgccg agccagtaga gaatgaccgc ggtgatgagg ctcgagatcg
     2641 aggcgtcgcg caccggcgcc cacgccctat ggaagccttc gtgaatggac tggaagagct
     2701 ttttgccgct ccgcaattcg tccttgagcc tctcgaatat gatgacgttc gcgtcgatcg
     2761 ctatgcccat tgatatgatg aagcccgcga tgcctgctgc cgtgagggtg atgccgaaca
     2821 gcttgaagag cgtgagcgaa atgccgacgt agagaagaag cgccgcagct gcgaggaaac
     2881 ccggcacgcg gtagagcacg agaaggaaga tgacgacgag aatgaagcct acgatgccgg
     2941 ccttgaaacc gagatccttc gcctgggttc cgagcgtcgc cccgacgacc tgggtcgatg
     3001 cgaggttgat cggcaccgga agggcgccgt agttcaggtt gcggacgagc gtcttcgctt
     3061 cgtcaggcgt gaaattgccg ctgatctggg cattgccgtc gggaatgggc tcattgatga
     3121 ccggcgacga tatgagctca ttgtcgagga agataccgag cactttgccg acgttgtttt
     3181 tcgtaagatc ggcgaagagc tgcctgcctt ggtcgttgaa ctggagcgtc acggtcggct
     3241 ggttcgccag ctgcgttcct ccgcccgaac taaacgtgat ctgcgcgtgc tgaaggaggc
     3301 cgccggtaag gccggtcggc tggaaaagct ccggatggtc ggcaagcgtc gaggtgccga
     3361 gcttgtcggc gagttcctgg tccgtgtacg gaagaagctt gaattcgagc gacggcgtct
     3421 ggccgatgat gccgatggcc ttttccgtat cggtcacgcc cggaaggtcg acgacgaggc
     3481 ggtgttcgga atttcccgaa atgatgcccg cgtcctcgac gcggacggac gcctcggata
     3541 cgccgaaggc gttcacgcgg cgctcaatga cgtcgcgaag cgtcgacatc gcgccggaca
     3601 cgtctccgga ctggagcttc gagacgtcgg cgttataggt gagctcggtg cccccgttaa
     3661 ggtcgagccc gtagcggaac ggaaagcccg aaaatgcgtt gccgggatgc tccgatgcgt
     3721 acaggaagaa gccgagcagt atcaccagag caacgagaaa taccgaaaaa attctgtgtt
     3781 ttatcatagt tcgctcataa atatacaacc gcaatactat cccaaaggcc gcctgaaatc
     3841 aatcgttttc ttcccctttc cccggaaacc cggtcaggct aggcggtgtt cacatatatc
     3901 gcggtacccg ctggggaaag ctttgatttt taagcgaaaa acgagctgga gcccgaagcg
     3961 atgccaaagc atcggagagg gcgaagcgaa gttttgcagc aaaaaatcaa agctttcctg
     4021 cgggggaata tatgtaaaca ccgcctaatt gcagcgccaa aaatcgagca gattgcag
//
LOCUS       DASZYS010000004        17533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1098355, whole genome shotgun sequence.
ACCESSION   DASZYS010000004 DASZYS010000000
VERSION     DASZYS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17533
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098355"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VHF05_00260"
     CDS             complement(<1..923)
                     /locus_tag="VHF05_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HVS79397.1"
                     /translation="MRNKFFILLAAIMLSATLGLSSVSAATFERTLSVGSTGPDVSVL
                     QQILKNLGYFTYSAITGYFGPVTQTAVQAFQAAKNIVSSGTPETTGYGVVGPQTRSVL
                     NAIAGTATTTPVAIADSTIFARDLEVGMTGDDVAALQRFLNAHGFLIASAGAGSSGNE
                     TNYFGPATQAALIRFQAANNIQPAAGYFGPLTRAKTAELSTAGPTAAGSASSLAAPAL
                     ITIEKTRSYEIVLSWSAVEGAASYTVERKGDNDASWKVIASGITATTYTDTTVQTDED
                     YSYIIDAVNAKGAGSPSQAADATARTPKSVR"
     gene            1086..1685
                     /locus_tag="VHF05_00265"
     CDS             1086..1685
                     /locus_tag="VHF05_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HVS79398.1"
                     /translation="MRKIIIQEFITLDGVMQAPGGPDEDASSGFKYGGWTAPYFMEAD
                     QAAPEFMKKWMESTDILLGRKTFDIFAAYWPTHADMWPGINDVAKYCVSATAEKSDWN
                     NTAFLKNVEDIKNLKNSEGGPLKVHGSGNLAQTLFKNDLVDELCLMTFPITLGQGKRL
                     FGEGTIPAAFKMLDSMVAPNGVIFAYYERAGEVKTGTVG"
     gene            complement(2114..2185)
                     /locus_tag="VHF05_00270"
     tRNA            complement(2114..2185)
                     /locus_tag="VHF05_00270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2150..2152),aa:Glu,seq:ttc)
     gene            2272..2358
                     /locus_tag="VHF05_00275"
     tRNA            2272..2358
                     /locus_tag="VHF05_00275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2306..2308,aa:Ser,seq:cga)
     gene            complement(2579..3784)
                     /locus_tag="VHF05_00280"
     CDS             complement(2579..3784)
                     /locus_tag="VHF05_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79399.1"
                     /translation="MHIQRKRKIAALVALSAAFVLANMSGLDTNGVQAAYSSDSPAGF
                     GGHLNFDLDSRIDDHVGTVSGQAERVLENALAWRTADERSGNESDASRTGWRNRDWSM
                     SDPAGRGDVSFWHHTRNDEGDSSDDVATSSGDQSDIPDENMNDGGESAASTSEEATST
                     DANDNPETAPDENTDDNNEEQAQGIAMVSLDFDDGWESAYQKATPILDAAGYKGTYFI
                     ITSAVGEGNYMSWDDIQNLEGSGNEIGDHTRTHPDFSEIGDNKAKQEIDEGYSDLQDE
                     GITPDSFAYPYGHYDTDTADDVESEGMSNARTSGSGFKLNDSNSNPYALWGQSLTIDT
                     TWDEVKSNIDEAIKEGKWYIIVIHRVDEAENDQYSVSHELVQQIVDYLKENNVKVVTN
                     SEGVRDLGL"
     gene            3934..5109
                     /gene="tsaD"
                     /locus_tag="VHF05_00285"
     CDS             3934..5109
                     /gene="tsaD"
                     /locus_tag="VHF05_00285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVS79400.1"
                     /translation="MKILGIETSCDETAVAIVEGKGGLKAPYFKLLGHAIYSQVAIHE
                     QYGGVFPMLAKREHSKNLVPLLEKALKEAKMLGRPAKSKKAPPEMQKKIRALLEREPE
                     LAESFLALLPAIPRPKIDAIAVTVGPGLEPALWVGVNFAKALSIFWNLPVIPSNHMEG
                     HIVSVLLPEKKSRPESVSFPAVSLLISGGHTELVLVKAWANYRIIGETRDDAVGEAFD
                     KVARMLGLPYPGGPHISKLAEEDRKRRPKSAWKLPRPMIGTDDFDFSFSGLKTAVLYS
                     VKNLPDLDKDSVRGLAREFEDAATEVLVAKTRKAIEKFGAKSLILGGGVTANARIRHA
                     FKALAADFPGLKLFLPAPSLSTDNAVMIAITGFIEAAKSRKLSLSRTFKADGNLKLS"
     gene            complement(5244..7013)
                     /locus_tag="VHF05_00290"
     CDS             complement(5244..7013)
                     /locus_tag="VHF05_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HVS79401.1"
                     /translation="MASSHAAQAAETTASGSGTITICTMIWNGNDGAFATSSDNLPDG
                     TFKMGVRYVGTDKNAPDLSESTSTVSFKAQTFAPNTKIGDTQDDNAQCVTWDEQDFGY
                     YWYGQESITGTTATISTDMQWNEPLYNDQYNASVKSFSDFQEFTTEKASSTDDSSLFD
                     SDGVISIGANRADRTLVVMNWFGSTTPPSSSNGGSSNGGSSSNGGGSSHHSSGTSSSS
                     SSGTGGSGDLSCTPYLNEYLKAGWQNDPVEVKKLQIFLSSYEGYSVSATGVFDTATFN
                     AVEAFQKKYASDILTPWGYSPDHATGFVYILTRNKINEIICGSNIPLTDLQQREIDQY
                     KSVYQQFLRTPSVSTPSATPAPQASQPTPIAPSITPTGTTTAPSETPASAPTIPNGGT
                     NTPGGGGLVVDQTGNGSGTVSTTSTSTGSLGTSTMGNLVSSIFSLPQSGTDLTRCIFN
                     LIIILAIIYIITSILVNSEDTTEEARSKTLTRKLWFYLAGSVIAIIFALFFGMYCLVV
                     PLGIVLIALIVILIVRQVRKGGSGGAAVAASAAAAPAKPQETEKSAPAPETKPEPSKE
                     TPKSEPAVPPATPETDMQDDASE"
     gene            7693..9882
                     /locus_tag="VHF05_00295"
     CDS             7693..9882
                     /locus_tag="VHF05_00295"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HVS79402.1"
                     /translation="MNDKFLTPYNPQETEPRIARAWEDSGFFNPEKMIAAGIIPSDAP
                     AFSMVLPPPNVTGTLHLGHAMMLAVQDIMVRFHRMKGDKTLWLPGTDHAAIATQSKVE
                     KNLQKEGVRKHDLGREEFLKRVEEFAQASHDTIVSQVRRMGSSVDWSREAFTLDKIRE
                     RAVRTAFKRMYDDGLIYRGDRIINWDPKGQTTISDDELVYVDEKTKFYYFKYGPFTIG
                     TARPETKFGDKYVVMHPDDKRYAEYRNGQKLELEWINGPITATVIKDEAIDMEFGTGV
                     MTITPWHSAIDFEIAERHELDKEQIIDQYGKLLPIAGEFAGLKISEARPKIVEKLESK
                     GLLVKVEEDYMHRVATAERTGGIIEPQIMKQWFVDVKKEFTILHSKLSGIADGQKATL
                     KELMRAAVENGQIKILPDRFEKVYFHWIDNLRDWNISRQIWYGHRVPVWYKGDKIHVG
                     VEAPQEEGWEQDPDTLDTWFSSGLWTFSTLGWPDETDDLKTYHPTTILETGYDILFFW
                     VARMILMSTYLLGDIPFKTVYLHGLVRDAQGRKMSKSLGNIIDPVDMADKYGADATRM
                     ALIIGTGPGNDIRLSEDKIKAYKLFANKLWNITRFVLSNSEDFDPQDDSSGMHAEWTD
                     EFENILKDVTSDIENYRFYLAGEKLYHYVWHRFADQIIEESKAMLQDPQKRRQTQAAL
                     RKILADSLKALHPFMPFITEEIWSLMPGNGGKPEDMLMVEKWPVKVQ"
     gene            9887..10591
                     /locus_tag="VHF05_00300"
     CDS             9887..10591
                     /locus_tag="VHF05_00300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HVS79403.1"
                     /translation="MTLADTWIFAGAGGLLPAMLWLLFWLREDAKHPEPKVRIASTFL
                     AGMVAVIAVLPLERATARIVSPNSPEMFLVWAFFEESLKLLACGIVALRSRDVDEPID
                     PIIYMITAALGFSALENALFLLNPLAHGQILETIITGNMRFLGANLLHVISSSAIGVF
                     MALSFYGRKMWKRISIFAGLLAATALHTLFNLFIIKETNNTAFATFGVVWLAIIVLMI
                     LFEKVKSIYPRKKKYA"
     gene            10584..11099
                     /locus_tag="VHF05_00305"
     CDS             10584..11099
                     /locus_tag="VHF05_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVS79404.1"
                     /translation="MHRDKRSFFERLTGAVKIDEPDEEASEEEYEEREEPTAPATFRQ
                     QSVDHQGMAQWAQEEEEEGQLSVDVYQDNNDIVIKTMVAGVKPEDLEVSISRDMVTIK
                     GRREQGRTIRDEDFFHQELYWGTFSRTIVLPHEIDPDGAEAMEKYGLLIIKLPKLDKN
                     RSTRLRVKSTQ"
     gene            complement(11301..11756)
                     /locus_tag="VHF05_00310"
     CDS             complement(11301..11756)
                     /locus_tag="VHF05_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79405.1"
                     /translation="MHRFRLLLAIFAMTALSLAAWGAMNPALRKVAWYGPVRWLHGAR
                     KGSDGAYYMKKGAYVYVADQYGNPTLSGFDEFRRLSNGARVGLNGPLQFLVDNEGKRM
                     SCGFDSFTPLKHGEYRARLGSAVFRLNSNGAIVWTRDHSGDSAVLTCPP"
     gene            11923..11995
                     /locus_tag="VHF05_00315"
     tRNA            11923..11995
                     /locus_tag="VHF05_00315"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11956..11958,aa:Phe,seq:gaa)
     gene            complement(12052..12294)
                     /locus_tag="VHF05_00320"
     CDS             complement(12052..12294)
                     /locus_tag="VHF05_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79406.1"
                     /translation="MNGERDLLERILDLTEENNKILRKIERANRWSLIFRIIYWLFVI
                     GAAVGIYYYIQPYQESLIHAYQTLQSFVDQMHHLGQ"
     gene            complement(12741..12812)
                     /locus_tag="VHF05_00325"
     tRNA            complement(12741..12812)
                     /locus_tag="VHF05_00325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12777..12779),aa:Arg,seq:cct)
     gene            complement(12903..13142)
                     /locus_tag="VHF05_00330"
     CDS             complement(12903..13142)
                     /locus_tag="VHF05_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVS79407.1"
                     /translation="MTNVEIYSTPTCIHCGHAKEFLKEHNVPYTEYNVLADVERRKEM
                     VDITGQMGVPVIVVGENVMVGFNQSKLAELVGVPA"
     gene            13339..13773
                     /locus_tag="VHF05_00335"
     CDS             13339..13773
                     /locus_tag="VHF05_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79408.1"
                     /translation="MTEDERRIHEDTTSTRNADLVALWTVNGIFSTTHFAGLVFTMSQ
                     ITERLVLMITCSFGVLLGIFWLLAAYTMWDWLAHWNNGLIALQEVVDERIRIFGDQKY
                     NQKSKRLRMSQVMTFLIIVIMYAWFILFLLGLKGILPIQKKG"
     gene            complement(14052..14122)
                     /locus_tag="VHF05_00340"
     tRNA            complement(14052..14122)
                     /locus_tag="VHF05_00340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14088..14090),aa:Gly,seq:ccc)
     gene            complement(14382..14831)
                     /locus_tag="VHF05_00345"
     CDS             complement(14382..14831)
                     /locus_tag="VHF05_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVS79409.1"
                     /translation="MLQYNEKTKLRRRIYSIPVFVVLLVITILVVRGTWDVYWKRVES
                     RQNLDKVTEQAAALESRQAELNDEIDRLQTQAGVEEEIRKKFSVVKDGEQVAVLVDNG
                     ASTTPAPPPKPGFWKKMWMAVTGMWGGNGNTAAATETPAEAIGTSTE"
     gene            complement(15227..17014)
                     /gene="lepA"
                     /locus_tag="VHF05_00350"
     CDS             complement(15227..17014)
                     /gene="lepA"
                     /locus_tag="VHF05_00350"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HVS79410.1"
                     /translation="MNIRNFSIIAHIDHGKSTLADRMLEVTNTIEKRKMRDQVLDNME
                     LERERGITIKMQPVRMKHSFEGREYVLNLIDTPGHIDFSYEVSRALKAVEGSILLVDA
                     TQGVQAQTLTTLAMARESGLVIIPVLSKIDSPLARKDEVKEEIVKLIGCKPEDVLETS
                     GRTGEGVAHLLDEVIRRVPAPTVPVGGKFRSLVFDFKYSNHKGVIVYVRVMEGKVSKG
                     EQLLFKVSNEKFSSLDTGVFMPDETPTPSLEAGEIGYIVTGVKEPGVASVGDTVALWG
                     DTAAPLPGYEKPRPVVWASIYPESQDDFNTLRQALGRLKLSDSSFVFEEESSGALGRG
                     FRCGFLGMLHLEIITERLRREFTLELIVTSPSITYEVELKNGKKEMVYSPSFFPDEGN
                     VAKIYEPWTKVSIITPTEYLGALMQLLYEHEAVVGMTENFGSDRTTVSIDMPLRELMR
                     NFFDKVKSASSGYASISYEMGEMKPADVVRLDILVADEVIPAFSRVVSRRLAEEEAEH
                     AVEKLHGILPKQMFVMKIQGRALGRIISSRTVSAYSKDVTQHMYGGDITRKMKLREKQ
                     KKGKKKMKERGKGSVNIPHDVFLKMMRNN"
     gene            complement(17172..>17533)
                     /locus_tag="VHF05_00355"
     CDS             complement(17172..>17533)
                     /locus_tag="VHF05_00355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79411.1"
                     /translation="KFNFELSGLEDFNFPKNQFDLVSAQRVLPFLQSKESLVKVFEKI
                     KISLKPNGIFAGHFFGPNDTWCKEKREMTFVTESEIRELLKDMEITKLQEFERDSQTA
                     TGTPHHWHIFDVIAGKK"
BASE COUNT         3986 a         4795 c         4589 g         4163 t
ORIGIN      
        1 ctcctgacgc ttttcggagt ccgggctgtc gcgtctgccg cttgcgacgg actgccggcg
       61 cccttcgcat ttacggcgtc gataatatat gaataatcct catcggtctg caccgtcgta
      121 tcggtatagg tcgtggcagt gatgccggaa gcgatgactt tccagctcgc gtcgttatct
      181 cccttgcgct cgacggtata ggatgccgca ccctcgaccg ccgaccacga caggacgatt
      241 tcatagctcc gggtcttttc tatggtgatc aacgccggtg cggcaagcga tgacgcgctt
      301 ccggcggcgg tcggtccggc cgtagacagc tcagccgttt tcgcgcgcgt cagcgggccg
      361 aaatagcccg cagccggctg aatattgttc gcggcctgaa agcggataag cgccgcttgc
      421 gttgctggtc cgaagtagtt cgtttcgttt ccggaggatc ccgcgccggc ggaggcaatg
      481 agaaaaccat gcgcattaag aaagcgctga agcgccgcga catcgtcccc cgtcatgccc
      541 acttcaagat cgcgggcaaa aatggtgctg tcggcgatgg caaccggagt cgtcgtcgca
      601 gtgcctgcga tcgcattgag cacgcttcgc gtctgcggac cgacgactcc gtagcctgtc
      661 gtttcgggag tgccggagga tacgatgttt tttgccgcct ggaacgcctg taccgccgtt
      721 tgggtgaccg gaccgaagta gccggtgatg gcggaatacg tgaagtaccc cagatttttc
      781 aaaatctgct ggagaacgga aacgtcaggg ccggttgatc cgaccgaaag cgtccgctcg
      841 aatgtggcgg ccgagacact tgaaaggccg agggtcgcgg agagcatgat cgccgcaagc
      901 aagataaaaa atttgttccg catatcgtcg tcgtgcaaaa agcgctaaaa ccaggtaatg
      961 atataaaaat catatcatac tgggcggcct gcaatgcaca tggattcatg aaggcatgga
     1021 aaatcaatag gcggcttttt aaccattatc ccgacctttt catcaaagtc tggtagtatg
     1081 gaagtatgag aaaaataatc atccaggagt tcattacttt ggacggagtg atgcaggcgc
     1141 cgggcggccc cgatgaggat gcgtcgagcg gcttcaaata cggcggctgg accgcgccgt
     1201 attttatgga agcggatcag gcggctcccg agtttatgaa aaagtggatg gaatccaccg
     1261 acatcctttt gggccggaag acgttcgata tcttcgccgc gtactggccg actcacgcgg
     1321 acatgtggcc gggcatcaac gacgtcgcca aatattgcgt cagcgcgaca gcggaaaaat
     1381 cggattggaa caacacggcg ttcctgaaaa atgtcgagga catcaagaat cttaaaaatt
     1441 ctgaaggcgg ccccctcaag gtccacggta gcggcaatct cgcgcagacg ctcttcaaga
     1501 acgatctcgt cgacgagctg tgcctcatga cgttcccgat cacgctcgga caaggcaagc
     1561 gcctgttcgg cgaaggcacc attccggcgg ccttcaagat gttggacagc atggtagcgc
     1621 cgaacggcgt catcttcgca tattacgagc gcgccggtga ggtaaaaacg ggtacggttg
     1681 gttaaacacg attactaatt tatacgggga atcggtcacg aataattctt gctccgtcga
     1741 gcaagaattt tctcgaatct tacagattct gtccactcat tcgctctcgc cgactcgagc
     1801 tgaatgagtc ttgcggaagc tgcgctaatc ctcgcttcct cacctgcctc gcgaccagct
     1861 ctgctgagct ggtacccgct ccggctttcg gttatcaatg tggtttgtgg aatcggtcac
     1921 aggtttctgc tcgcagccgc ttgtgatgga atgctccgca ttcaaaatgc acatggccca
     1981 caactctcac ctcgccggcc gctcggttcg gtcgccggcc ccggctcgcg gtttgctctg
     2041 ctgagcaaac atcgctccgc cttcacaaaa cgaagaaaag ctccacaagg agcttttctt
     2101 gaatcacgtt ttgtgaccct acggggaatc gaaccccgat tctgggcttg aaaagcccat
     2161 gtcctaaccg ttagacgata gggccgtatt tggcggactt aatatagcaa aatcagccga
     2221 aatctgctag catagtcgtg ttcagtaaag agtatctata gaagtagact tggagacgtg
     2281 gctgagtggt cgaaagcgcc gcactcgaaa tgcggtatct gggcaaccgg atcagaggtt
     2341 cgaatcctct cgtctccgcc gttacgaata agagcatccg tcaggatgcc ttttcgtcgg
     2401 cggagacgaa caggccaact gcttggcctg cgaagaggat gagaaaaacc tcgagcatat
     2461 ttctggctgg cttttgcagc cagaaatgcg aaaggtgtac agaatctgta agattcgaat
     2521 cctctcgtct ccgcaaataa aaaacgagcc gttatgcaac ggctcgtttt ttattctcct
     2581 acagccccaa atctctcacg ccttcggagt tcgttacgac tttgacgtta ttttctttga
     2641 gataatcgac gatctgctgg acgagctcat gggaaacgct gtactggtcg ttttccgctt
     2701 cgtcgacgcg atggatcacg atgatatacc atttcccctc cttgattgcc tcgtcgatat
     2761 tgcttttcac ttcatcccac gtcgtgtcga tggtaagcga ctggccccag agggcatacg
     2821 gattgctgtt cgagtcgttc agcttgaagc ccgagcccga ggtgcgagcg ttcgacatgc
     2881 cttcgctttc cacatcatcc gcggtatcgg tgtcgtagtg gccgtatgga tatgcgaacg
     2941 aatcgggagt aataccttcg tcttgcagat ccgaatagcc ttcgtcgatc tcctgtttcg
     3001 ccttattgtc tcctatctct gagaaatccg ggtgcgtgcg cgtatggtcg ccgatctcgt
     3061 tgcccgaacc ttcaaggttc tgtatgtcgt cccacgacat gtaattgcct tcgccgacag
     3121 ccgaggtgat gatgaaatac gttcccttgt acccggcggc gtcgagaatg ggcgtggctt
     3181 tctggtatgc cgactcccat ccgtcatcga aatcgaggga aaccatcgcg atgccctgcg
     3241 cctgctcctc gttattatca tcagtatttt catcgggagc cgtctcggga ttgtcattgg
     3301 cgtcggtcga agtcgcttct tcgcttgtcg aagcggcact ctcgccgccg tcgttcatat
     3361 tttcatcagg aatgtccgac tgatcgccgc ttgaggtcgc tacatcgtcc gatgaatcgc
     3421 cttcatcgtt ccgagtatga tgccagaatg aaacgtctcc cctccccgcg gggtccgaca
     3481 tgctccaatc ccgattccgc cagccggttc gggacgcatc gctttcgttg ccgctcctct
     3541 catcggcagt gcgccaggca agcgcgtttt cgagcacgcg ttccgcctgg ccggagaccg
     3601 tccctacatg atcgtcaatg cgtgaatcaa gatcgaaatt caggtgcccg ccgaaaccgg
     3661 caggcgaatc cgatgaatac gccgcctgga cgccgttcgt atcgagccca gacatgtttg
     3721 cgagcacaaa agccgccgaa agggcgacaa gcgcggcaat ctttctcttc cgttgaatgt
     3781 gcattgtttt attgatgatt atgcgggaat gccgtgtact gaattttccc taccgcttgt
     3841 tatatcagcc aagcatgacg ctcgcattaa gaaaatcggc cggcatatgc ggcgcgtttg
     3901 acctccgttc atctttctca tatctttaat gaaatgaaga tcctcggaat cgaaacgagc
     3961 tgcgatgaaa ccgcggtcgc gatagtcgaa ggcaaaggcg gcctcaaggc gccgtatttc
     4021 aagcttttgg gccacgcaat ctactcccaa gtggccatcc acgagcaata cggcggcgtc
     4081 ttccccatgc ttgccaaacg cgagcattcg aaaaacctcg ttccgctcct cgaaaaggcc
     4141 ctcaaggagg cgaagatgct cggccgtccg gcaaaatcca agaaagcgcc gcccgaaatg
     4201 cagaagaaaa tccgcgcgct cttggagcgc gagcccgagc ttgccgaaag ctttctcgcg
     4261 cttcttcccg ctatcccgcg gcccaaaatc gacgccattg ccgtcaccgt gggccccggc
     4321 ctcgagccgg ctctctgggt cggcgtcaat ttcgccaagg cgctctcgat attctggaat
     4381 ctgccggtca ttccttcaaa ccacatggaa ggccacatcg tgtccgtgct cctgcccgag
     4441 aagaaaagcc gccccgaatc ggtttccttt ccggcagttt ctcttcttat ttccggcggc
     4501 cacaccgagc tcgtgctcgt aaaagcttgg gcgaactacc ggatcatcgg cgaaacgcgc
     4561 gacgacgcgg taggcgaagc cttcgacaag gtggcgagaa tgctcggcct cccctatccc
     4621 ggcggcccgc acatctccaa gcttgccgag gaagatcgca agcgccgtcc gaaaagcgcc
     4681 tggaaactgc cccggccgat gatcggcacg gatgatttcg acttttcctt ttccggcctt
     4741 aagaccgccg tgctctactc ggtgaagaat cttcccgacc tcgacaagga ttccgtgcgc
     4801 ggcctggccc gcgaattcga agacgccgcg accgaagtgc tcgtcgcaaa gacacgcaag
     4861 gcgatcgaga agttcggcgc caagtctctt attctcggag gcggcgtgac cgccaacgcc
     4921 cgtatccgcc acgccttcaa ggctttggcc gccgatttcc ccggcctgaa gctcttcctg
     4981 cccgctccga gcctttcgac cgacaatgcc gtcatgatcg ccattaccgg ctttatcgaa
     5041 gcggcgaaaa gcaggaagct ctccctttcc cgaactttca aggctgacgg caatctcaaa
     5101 ctttcttaag aagcttatag ctgataggtt gtagggggag aagaaaccgg ggaatttttg
     5161 aagaatagaa aaaccgcccc gtgagatgtc gcgctggacg cttcttcacg gggcggtttt
     5221 ttgtacagtc ttacgggatc aagctactcg gaagcgtcgt cctgcatgtc ggtttcgggg
     5281 gtcgcaggcg gaacggcagg ttcggacttg ggcgtctcct tggaaggctc gggtttcgtc
     5341 tcgggagcgg gtgccgattt ttcggtttcc tggggctttg caggtgccgc ggcggcgctt
     5401 gccgccacag ctgctcctcc actgccgccc ttgcgcacct ggcggacgat gaggatcacg
     5461 ataagggcga taaggacgat gccgagcgga acgacaaggc aatacattcc gaaaaagagg
     5521 gcgaagatga tggcgataac cgatccggcg agatagaacc atagcttgcg ggtaagcgtc
     5581 ttggaacgcg cttcttcggt tgtgtcctcg ctatttacaa ggatgctcgt gatgatatag
     5641 atgatcgcga gaatgatgat gagattgaag atgcagcggg tgaggtcggt gccggactgc
     5701 gggagtgaaa agatcgagga tacgagattt cccatcgtgc tcgtaccgag gctgccggta
     5761 gaggtcgagg tggtggaaac ggtgccggat ccgttgccgg tctggtcgac aacgagaccg
     5821 ccgccgccag gagtattcgt gccgccgttc gggatagtcg gagcggaagc gggggtttcg
     5881 gaaggagcgg tcgtcgttcc ggtcggagtg atgctcggag cgatgggagt aggctgcgag
     5941 gcctgcggcg cgggcgtagc ggaaggagtg gaaacggaag gagtgcggag gaactgctgg
     6001 tatacgctct tgtactggtc gatttcgcgc tgctgcaggt cggtcagcgg gatatttgat
     6061 ccgcagatga tctcattgat cttgttgcgg gtgaggatgt atacgaagcc ggtcgcgtgg
     6121 tccggggagt agccccacgg agtgaggatg tccgaagcat atttcttttg gaatgcctca
     6181 accgcattga aggttgcggt gtcgaagacg ccggttgccg aaacgctata tccttcatag
     6241 ctcgagagga aaatctggag cttcttcact tcaaccgggt cgttctgcca gccggccttc
     6301 agatattcat taaggtacgg agtgcaggac aggtcgcccg agccgccggt gcccgacgat
     6361 gaagaagagc tcgtgccgga cgaatgatgc gatgacccgc cgccattcga ggatgaaccg
     6421 ccgttcgaac tgccgccgtt ggaggagctc ggaggagtcg tcgaaccgaa ccagttcatg
     6481 acgacaaggg tgcgatcggc gcgattcgcg ccgatcgaga tcacgccgtc cgaatcaaat
     6541 aaagaggaat catcggtgct cgaggccttc tctgtggtga actcctggaa atccgagaag
     6601 ctttttaccg aggcattgta ctgatcatta taaagaggct cgttccactg catgtcggtc
     6661 gagattgtgg cagtcgtgcc ggtgatggac tcctgtccgt accagtagta gccgaagtct
     6721 tgttcgtccc aagtaacgca ctgcgcgttg tcatcctgcg tatcgccgat cttggtattc
     6781 ggggcgaacg tctgcgcctt gaaggaaacc gtcgatgtac tctcgctgag gtcgggtgcg
     6841 ttcttgtcgg tgccgacata gcggacgccc atcttaaaag tgccgtcagg gagattgtcg
     6901 ctcgatgtcg cgaatgctcc gtcattgccg ttccagatca tcgtgcagat cgtaatggtt
     6961 cccgatccgc tggccgtcgt ctcggccgcc tgagctgcgt gcgatgaagc catgaatgca
     7021 aacgcgaaaa tcgcagcgaa ggaaacgatc cctgcaaccc atccgcgcag catgccagcc
     7081 gaattcttaa acttccaatt catgtatgtt gcgtaatgct gataatttct tattccgtta
     7141 cctgtcttct cactccctcc gcataatata tatatatggt gcggatgcta tggcttccac
     7201 aatagtttat acggctccac tgtcaagcaa aaaatcgtgt cgtaaaaatc tcaattcggc
     7261 ttgtggttga gccattttaa ttatgtcctt tatatttttt tgtcctttat aatcagctgt
     7321 cttttatagg acatttttgc aaagacttaa aggccatttt ttgcacctcg ccgaacttct
     7381 tttggactgt gaaatttcat gtgtctttta gagtgctgtc tttttagatt ttcgagataa
     7441 atttgaccga atatcttttt tgtattcgaa agtttgaaag cgcttgcagc acgccaatcc
     7501 caaggagaat ttgcttttgt gctatcattc atggtactca ttgagtaagc gagcgcagag
     7561 cctcatgaat cccgttagag gataattcag ttattattgg attctactaa aatgcgcgac
     7621 tattcgcggt cgatatgcct acaaaacacc aattaaggac ggaaacggcg gccgtttcct
     7681 atctctaacg gaatgaacga caaatttctc actccttata acccccagga aaccgagcct
     7741 cgcatcgcgc gcgcatggga ggacagcggt ttttttaatc ctgaaaaaat gatcgcagcc
     7801 ggcatcatcc cctccgatgc gccggcattt tcgatggtgc tccccccgcc caacgtgaca
     7861 ggtacgctcc atctcggtca tgccatgatg cttgcagtgc aggatattat ggtgcgcttc
     7921 catagaatga agggcgacaa gacgctctgg ctcccgggca ccgatcatgc cgcaatcgcc
     7981 acccagtcca aggtcgagaa aaatctccag aaggaaggcg ttcgcaagca tgacctggga
     8041 cgcgaggagt tcctgaaacg cgtcgaggag ttcgcccagg cgagtcatga cacgatcgtg
     8101 agccaggtgc gcaggatggg ttcgtcagtc gattggagcc gcgaagcgtt tacgctcgac
     8161 aagatccgtg agcgcgcggt ccgcaccgcc ttcaagcgca tgtacgacga cggccttatt
     8221 taccgcggtg atcgcatcat taattgggat ccgaaaggtc agaccaccat ttcggatgac
     8281 gaacttgtat atgtcgatga aaagaccaaa ttctactatt tcaagtatgg tccgtttacc
     8341 atcggaaccg cgcgtcccga aacgaagttc ggggacaagt atgttgttat gcaccctgac
     8401 gataagcggt atgcagaata ccgtaacggc cagaaactcg agcttgaatg gatcaacggc
     8461 ccaattacgg caactgtcat caaggatgag gcgattgaca tggaattcgg taccggcgtc
     8521 atgacgatca cgccgtggca cagcgccatc gacttcgaaa tcgcggagcg ccacgaactc
     8581 gacaaggaac agattatcga tcaatacgga aaattactgc caatcgcagg cgaatttgcc
     8641 gggttgaaga taagcgaggc acgcccgaaa atcgtcgaga agctcgaatc caaaggattg
     8701 ctggtaaaag tggaggagga ttatatgcac cgcgtcgcga ccgccgaacg gaccggagga
     8761 atcatagaac cgcagatcat gaagcaatgg ttcgtggatg tgaaaaagga atttactatt
     8821 ctccattcga aactttcggg aatcgccgac ggccagaaag ccaccctgaa agagcttatg
     8881 cgcgcggcgg ttgagaacgg ccagatcaaa atcctccccg atcgtttcga gaaagtatat
     8941 ttccactgga tagacaatct ccgcgactgg aatatcagcc gtcagatctg gtatggtcac
     9001 cgcgtgccgg tgtggtacaa gggcgataaa atacatgtcg gagtggaagc gccgcaggaa
     9061 gaaggttggg agcaggaccc cgacacgctc gacacatggt tctcttcagg cctttggaca
     9121 ttctcaacgc tcggctggcc cgacgaaacg gatgatctga aaacatatca cccgacgact
     9181 attctcgaaa ccggctacga cattctcttc ttctgggtgg cgcggatgat ccttatgtcg
     9241 acatatctcc tgggagacat tcctttcaag accgtgtatc ttcacggcct cgttcgcgac
     9301 gctcagggcc gcaagatgtc aaaatctctc ggtaatatca tcgatcctgt agatatggcc
     9361 gacaaatacg gcgcagatgc cacgcgcatg gcgctcatta tcggcaccgg tcccggcaac
     9421 gacatcaggc tctccgagga caaaatcaag gcctacaagc tcttcgccaa taagctctgg
     9481 aacatcaccc gattcgtcct ctcgaattcc gaagatttcg atccgcagga tgattcctcc
     9541 ggtatgcacg ccgaatggac ggatgaattt gagaatatcc tcaaagacgt aacttcggac
     9601 atcgagaact atcgtttcta tctcgccggc gagaagctct atcattacgt ctggcacagg
     9661 ttcgccgacc agatcatcga ggaaagcaag gcgatgctcc aggatccgca gaagcggcgg
     9721 cagacgcagg cggcattgcg aaagatcctc gccgattcgc tgaaagccct ccatcccttc
     9781 atgccgttca tcacggaaga gatctggtcg ctcatgcctg gaaatggcgg caagcctgag
     9841 gatatgctca tggtcgaaaa gtggccggtg aaggtacaat agaggaatga ccctagcaga
     9901 cacatggatc ttcgcgggag cgggcggcct gcttccggca atgctgtggc tccttttctg
     9961 gcttcgcgaa gacgcgaagc atccggagcc caaggtgcgc atcgcaagca cctttctcgc
    10021 aggcatggtc gcggtgattg cagtactgcc gctcgagcgc gcgacagcgc gcatcgtatc
    10081 gcccaactcg cccgaaatgt tcctcgtctg ggctttcttc gaggaatcgc tcaaactgct
    10141 tgcctgcggc atcgtggcgc tccggagccg cgatgtcgac gagccgatcg acccgatcat
    10201 atatatgatc acggcggcat tgggattttc cgcccttgaa aacgccctgt tcctgctcaa
    10261 tccgctcgcc cacggccaga ttctcgagac gatcatcacc ggcaacatgc gcttcctcgg
    10321 agcgaacctg ctccatgtca tctcttcgag cgccatcggc gtcttcatgg cgctctcatt
    10381 ctacgggcgg aagatgtgga agcgaatctc catttttgcg ggacttctcg cggcgacggc
    10441 attgcacacg ctattcaatt tatttataat taaggagaca aataataccg cattcgccac
    10501 cttcggggtc gtatggcttg caatcatcgt cctgatgatc ctgtttgaaa aagtaaaatc
    10561 aatttatcct cgcaagaaaa aatatgcata gggacaaaag atcgtttttt gaaagactga
    10621 caggcgccgt gaagatcgac gagccggatg aggaagcaag cgaggaggaa tacgaggaac
    10681 gcgaagagcc gaccgccccc gcaaccttcc gccagcagtc cgtcgatcat cagggaatgg
    10741 cgcagtgggc ccaggaagag gaggaagagg gccagctctc cgtcgacgtc taccaggaca
    10801 acaacgacat cgtcatcaag acgatggttg ccggcgtaaa gccggaagat ttggaagtgt
    10861 ctatttcccg cgacatggta accatcaagg gccgtcgcga acagggccgc accatccgcg
    10921 atgaggattt cttccaccag gagctctact ggggcacctt ctcgaggaca atcgtccttc
    10981 cgcacgagat cgatccggac ggcgccgagg cgatggaaaa atacggcctc ctcatcatca
    11041 agcttccgaa gctcgacaag aacagatcga ctcgcctccg agtaaaaagc actcaataat
    11101 tttttgcagt ctttttcccc agtctctgcg ttgcttactt gcgtttgcct gaagcggtat
    11161 tgctcataca gcttctgtgc aagccgcgtt cgcgccttga cactgtgaaa aaatcctagc
    11221 aaaatcaatc ttgcaattag ccggtttctt aaatacaaaa aagccgcgag agtctcgcgg
    11281 cttttattca tgaaaaaacg ctacggcgga cacgtcagca cggcggaatc tccgctgtga
    11341 tcgcgagtcc atacgatcgc cccgttcgaa ttgagccgga agacagcgct tccgagccgg
    11401 gctcgatatt ctccgtgctt caatggagtg aacgaatcga atccgcatga catgcgtttt
    11461 ccctcgttat cgacaaggaa ctggagcggg ccgttgaggc cgaccctcgc gccgtttgaa
    11521 agccggcgaa actcatcgaa accgctcaat gtcggattgc cgtattgatc ggcaacgtag
    11581 acataagcgc ccttcttcat gtaatacgcg ccgtcggagc cctttcttgc cccatgaagc
    11641 cagcgtacgg gcccatacca tgcgaccttt cgcagtgcag gattcatcgc accccatgcg
    11701 gcaagtgaca atgccgtcat agcgaaaatc gccaataaca acctgaagcg atgcatatcc
    11761 gcctcctttc acctcgctgt caaaattccc cttcacttta attatgcgta cagccgacct
    11821 atagcgtcaa cggagatatg caccgttccc aagcctgccg cctttcttgt acgaaggcaa
    11881 atttcttgat aggctcttat gtgttaggtt ctaaacctga ctgctggggt agctcagttg
    11941 gtagagcatt cgcctgaaga gcgaagggtc accggttcga ccccggtccc cagcactttg
    12001 gaatcaagtc tcggcaaaaa gaaaaagacg ggcattgccc gtctttttct tttattgccc
    12061 taaatgatgc atctggtcga caaacgattg gagagtctga taggcatgga tgagcgattc
    12121 ctggtacggc tggatgtagt aatagatgcc gacggccgcg ccgataacga agagccagta
    12181 gatgatcctg aaaataaggc tccatcggtt tgcccgttcg attttgcgca ggattttatt
    12241 gttctcctcg gtcagatcga gtatgcgctc cagcaggtcg cgttcgccgt tcatggacaa
    12301 aattatagca gaaaaacgaa aagaggcggc aagacgcttc gcattttgaa acatcagcag
    12361 gtcacaagtc ggataggctc cagtcgagcc tatccgctcg cgacccgggc tcgcgatctt
    12421 gcctgctggc tcgataacgc ttcgcctcac acatacgcac aaaagcccgc gcaggcgcaa
    12481 aatgtctctt ggtcaggaat attttcctcg ccgtcgctcg caaaatttcc gcgtcccagc
    12541 tctcgcgttg ctacgcgagg ccgcgacccc gcctcagccc gtcggcttcc ggccttgcga
    12601 agcgcttgcc cgcaatcgga attgcgggtt cccatatgcg cttctcaccc tggctcgcgg
    12661 cctcgtttgc taactcggca tcgctccgcc tcacatttcg cgaaaaaaca gtgcgcaggc
    12721 actgtttttt cgcctcaatg tgccctcggg cggaattgaa ccaccatcga ctcgttagga
    12781 gtgagccgtt ctatccgttg aactacgagg gcaatattta atttttcagc tccgtccgaa
    12841 atactgtacc acattctttg gcaacaaaaa acagcgccgt aaggcgctgt ttttgcattc
    12901 atttacgccg gaacgccgac caattcagca agtttcgact gattaaagcc aaccatcacg
    12961 ttctccccca ccacgatgac cggaacgccc atctggccgg taatatccac catttccttg
    13021 cgcctttcga cgtccgcaag cacgttgtat tcggtgtacg ggacattgtg ctccttcagg
    13081 aattccttgg catgcccgca gtggatgcaa gtcggagtcg aatagatttc tacgtttgtc
    13141 ataagtcttt gcttatatat tcttgggtat gggtattata gcacagtgcg ttatacacag
    13201 aaaaaattgt tccctaaatt tatttgacaa aattttaaaa aatgattaaa tatatcagga
    13261 aatgcacttc gacatttaca ggaggtaaaa atgccggaaa ctagcgaaac ggacccgaca
    13321 tacatcgaaa aacgaagcat gacggaagac gaaaggcgta ttcacgaaga tacaactagc
    13381 acgagaaatg ctgaccttgt tgccctttgg actgtcaacg gtatattttc gactactcat
    13441 tttgccggtc ttgttttcac aatgagtcag ataactgaac gcctcgtcct catgatcaca
    13501 tgcagctttg gggttttgct tggaattttc tggctccttg cggcatacac gatgtgggac
    13561 tggcttgcac attggaacaa tggccttatc gcgttgcaag aggttgtcga tgaaaggatt
    13621 cgcatttttg gcgaccaaaa atataaccaa aaatccaaac gactaaggat gtcccaggtc
    13681 atgacatttc ttattattgt gataatgtat gcatggttta ttttgttctt acttggcctc
    13741 aagggaatat taccgattca aaagaaagga tgagaagtat gttaaagaag aattccaatg
    13801 aagttctctt cagatggtat gacacgtgga tgctcctgcc gttcctgatt atcctctatg
    13861 tcgtcatcac aaagaccgct ccattgccag gagtaatcag attggcagta cttggcctcg
    13921 gtatcctttt gtctggaggc ttgattagcg cgcgagtagc cgcatatcgc gacgctcggg
    13981 ccggaagaca tcccccattt tgatctttct acccacgttc ccatgcaagc atgggaacgt
    14041 tttttttatt gtgcgggtag cgagaatcga actcgcgtct caaccttggg aaggtcgcat
    14101 tctgccacta aaccataccc gcatttttaa ggaagcacca gatcacggat aattttttgc
    14161 taaaaatttc cgcgaatctt acagattctg tccactcatt cgctctcgaa gactcgagct
    14221 gaatgagtct tgcggaagct gcctgctgtc gcttcctcac ccgcctcgcg ccgccgcgct
    14281 ccggccttgc gaagctccaa atattcgctt ctcactacca ctaaactata ctcgcaaaat
    14341 tttaaagaaa cactcgtttt gccactaaac tatggccgca tttattccgt cgacgtgccg
    14401 attgcctcag caggcgtttc tgtggcggcc gcagtatttc cattgccgcc ccacatgccc
    14461 gtaacggcca tccacatttt cttccaaaag ccgggcttcg gcgggggggc gggcgtggtg
    14521 cttgccccat tgtcgacaag gactgccacc tgctccccgt ccttcacgac gctgaacttc
    14581 ttcctgattt cttcctccac tcccgcctgc gtctggagcc tgtcgatctc gtcattgagc
    14641 tccgcctggc ggctttcgag cgccgcggcc tgctcggtca ccttgtcgag gttttgccgg
    14701 ctttcgacgc gcttccaata tacatcccag gtgccccgca ccacaaggat ggtgattacg
    14761 aggagcacga caaacaccgg tatggagtag atacggcggc gaagcttggt tttttcgtta
    14821 tattgaagca ttccgaagca tttggcagca aaatgagggc gtttccgcgc cctttcatgg
    14881 attgccggat tcctatatta tattagttga ggagcaattc tcaaaccttc atttaaggcc
    14941 actatatgct ttttcatccc aaaaaccgca aagccatcaa catcatctgg acgatccttt
    15001 ccattctcgt cgcagtcggc atggtagtcc tctatctgcc gattttccag caatagggac
    15061 gctcggaaat ctgattttaa gaacacgcat aaaatcgccc cactcgggcg atttttgctc
    15121 tgctcgtcgg attttctgct gaaaatcctc ctgcgcaagg ttttaaaatc agaattcctc
    15181 gctctcgtgt tctgaaggaa aagcccgtag acaggctttg cataatttag ttattcctca
    15241 tcatcttgag aaacacatca tgagggatat taaccgagcc cttgccgcgc tctttcattt
    15301 tcttctttcc cttcttctgc ttttcgcgga gcttcatttt gcgggtgata tcgccgccgt
    15361 acatatgctg ggtaacgtcc ttgctgtagg cggaaaccgt gcgcgacgaa atgatgcgcc
    15421 ccagcgccct gccctggatt ttcattacga acatctgctt cggcaatatg ccgtgaagct
    15481 tttcgactgc gtgctcggct tcctcctcgg caagcctgcg ggacaccacg cgggagaatg
    15541 cgggaatgac ttcgtcggcg acgagaatgt cgagacgcac gacatctgcc ggcttcattt
    15601 cccccatttc atatgagatc gaggcatagc cggaagaggc gctcttcacc ttgtcgaaga
    15661 agttgcgcat gagctcgcgg agcggcatgt cgatcgagac ggtcgtcctg tcgcttccga
    15721 aattctccgt cattcccacg accgcctcat gctcatagag aagctgcatg agagctccga
    15781 ggtactccgt cggcgtaatg atgctcactt tcgtccacgg ctcgtagatc ttcgccacgt
    15841 ttccctcgtc gggaaaaaac gacggcgaat acaccatctc cttctttccg ttcttgagtt
    15901 cgacttcata ggtgatggac gggctcgtaa cgataagctc gagcgtgaat tcccggcgca
    15961 ggcgctcggt gatgatctcc aagtggagca ttccgaggaa gccgcaccgg aagccgcggc
    16021 ccagggcgcc ggaagactct tcctcgaaca cgaacgacga atccgaaagc ttgagcctcc
    16081 ccagagcctg gcggagagta ttgaaatcat cctggctttc gggatagatc gacgcccaca
    16141 cgaccggccg cggcttttcg tacccgggaa gcggcgctgc cgtatcgccc caaagtgcca
    16201 cggtgtctcc cacggaagcg acgccgggct ccttgacgcc cgtaacgatg tagccgatct
    16261 cccccgcttc gagcgacgga gtcggcgtct cgtcgggcat gaagacgccg gtatcgagcg
    16321 atgagaattt ctcgttcgaa accttgaaaa ggagctgttc gcccttcgaa acttttccct
    16381 ccattacccg cacatagaca atgacgccct tgtggttgga atatttgaaa tcgaagacaa
    16441 gcgaacggaa cttgccgccg accggcaccg tcggcgcggg tacgcggcgg atgacctcgt
    16501 caagaagatg cgcgacgccc tcgcccgtcc tgccggacgt ttcaagcacg tcctccggct
    16561 tgcagccgat aagcttcacg atctcctcct tcacctcgtc cttgcgcgcc aggggcgaat
    16621 cgatcttcga gagcaccgga atgatcacaa gtcccgactc gcgcgccatc gcaagcgtcg
    16681 tgagcgtctg cgcctggacg ccctgcgtcg cgtcgacgag aaggatcgac ccttcgacgg
    16741 ccttgagcgc gcgcgagact tcatacgaga agtcgatatg gccgggcgtg tcgatgagat
    16801 ttaaaacgta ttcccttcct tcgaaggaat gcttcatgcg caccggctgc atcttgatgg
    16861 tgatgccgcg ctcgcgctcg agctccatgt tgtcgagcac ctggtcgcgc atcttgcgct
    16921 tctcgatcgt attcgtaacc tcgagcatgc ggtcggcaag cgtactcttt ccatgatcaa
    16981 tatgcgcaat gattgaaaag tttctaatat tcatggggat gatcgatgcc tgccccgtag
    17041 gaagctgtct tctgctttcc tgcggggtgc gctatgatgc tgaaattcct tatattcatt
    17101 ggctttgggt actcaaagca tactgatttc gaccactttt ggcaaaaaac cggctcgata
    17161 ggtaactatc gttatttctt tccagctatt acgtcaaaga tatgccaatg atgtggcgtt
    17221 cctgtagctg tttgggaatc cctttcgaac tcttgcagtt tggtaatttc catgtcttta
    17281 agcaattccc gtatttcact ttccgtaaca aaagtcattt cccttttctc cttgcaccag
    17341 gtatcgttgg ggccgaaaaa gtggcctgca aatatcccat ttggtttaag agagatttta
    17401 attttctcga ataccttaac aaggctttcc ttgctttgaa gaaacgggag tacgcgttgc
    17461 gcgctcacca gatcaaactg attttttgga aaattaaaat cttccaaacc gcttaattca
    17521 aagttgaatt ttt
//
LOCUS       DASZYS010000005         6340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_263797, whole genome shotgun sequence.
ACCESSION   DASZYS010000005 DASZYS010000000
VERSION     DASZYS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263797"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VHF05_00360"
     CDS             <1..246
                     /locus_tag="VHF05_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79412.1"
                     /translation="SQVNPPPFIILDETDAALDEANSKKYGDMIKNLSDKSQLIVITH
                     NRETMSRAGILYGVTMGRGGVSTLLSIKFEEAVAVAK"
     gene            complement(988..3174)
                     /gene="rnr"
                     /locus_tag="VHF05_00365"
     CDS             complement(988..3174)
                     /gene="rnr"
                     /locus_tag="VHF05_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HVS79413.1"
                     /translation="MDTNRNGGRGRNGNERGGNWRNDRDRRNGGNSGNFNGSQFSRNG
                     GNGGFERKFGPRPPLRPRRFPAPDASALSAPASSPLETAASQKPGITGIISVNGRGVG
                     YFPIEGREEDIEIQPENLNMALHGDTVEIGLLPKTHGVLREQGIVLRIIERAKMEFVG
                     TVDAAPEGTYLIPDDRKMYLDILIPADEAKGLSSGEKLLVKIVRWDSPKSNPIGAIVQ
                     DIGRKGMHETEIQSIILERGIDTHFPHAVDDEANELARTEHPIRPDEIAGRRDMRGTF
                     TFTCDPTDAKDFDDALSFKELGDGKFEVGIHIADVSHYVREGTELDREARKRGFSVYL
                     VDRTIPMLPEILSNDLCSLNPEEDKLAFSTVFIMNAEGQVEDRWIGKTVIRSDKRFSY
                     ETAQKSIDENGEHGDVLRRLNAIAKKLGEERKKAGAIDFDTTEIKFKLDKDGKPIEII
                     KKERLDTHKLVEEFMLLSNREVAEYITRLEKAAGGHGAAKRPFLFRIHNMPDKDRIEN
                     LTLFLKALGLNLGRPGKEVTAYDISRLLESIEGMPQEELIKTAAIRSMAKAIYSTRNI
                     GHFGLAFRNYTHFTSPIRRYPDLIVHRLLFKYLTDPAHVSEDFAKYERIAMESSEKEV
                     VAADAERASIKYKQVEFLKDRVGEVFDGVVSGVTEWGVYVEDPETHAEGMVRIKDIGD
                     DFFELDQKTYSLVGRRTGRRLSLGDKVKVKLVAADLDRKILDFKFV"
     gene            3282..3752
                     /gene="rpsP"
                     /locus_tag="VHF05_00370"
     CDS             3282..3752
                     /gene="rpsP"
                     /locus_tag="VHF05_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVS79414.1"
                     /translation="MLKIRLQRTGRKHEPTFRLVVTESQNAAKSGKALEVLGSYDARK
                     TNEIIHTDRVKHWIEKGAKPSDTVHNLLVSKKIIEGRKVNALPKKKPIIDQAKLDAEK
                     AAAEAAEAEKARAEAEAAAAAAAAEAPAPAEAPAEEAAPAEAPAETPSEETPAE"
     gene            3974..4291
                     /locus_tag="VHF05_00375"
     CDS             3974..4291
                     /locus_tag="VHF05_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVS79415.1"
                     /translation="MEHQDTQFLDFVIKALVDNPDKVKINRTVDEMGVLLTLDVDPID
                     MGKVIGRSGNTAKAIRTLLRVVGMKNNSRVNLKINEPEGGMRNESGKATKTVDEAMED
                     LSI"
     gene            4437..5099
                     /gene="trmD"
                     /locus_tag="VHF05_00380"
     CDS             4437..5099
                     /gene="trmD"
                     /locus_tag="VHF05_00380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVS79416.1"
                     /translation="MNFHVITLFPGAFDSYLGESILKRAIEDKKIKVHFYNPRDFTKD
                     KWRRIDRKPYAGGPGMVIEALPVIKAIEKAMQKSKIKNKKSKRNAKIFFLSPGGKQFT
                     NEIGEKAAKKYTDIIIVSGRYEGTDARIKKVFKMEDISIGPFVLTGGELPAMLMIDVI
                     ARRVPGVLGNIGSLEESRVSSHDVYTRPEVFEYKGKKYRVPKILLGGDHKKIEEWKKK
                     RK"
     gene            5124..5375
                     /locus_tag="VHF05_00385"
     CDS             5124..5375
                     /locus_tag="VHF05_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79417.1"
                     /translation="MASTKTVIISSIPDLEIGKIYLGLTKGPWYQPLPGQCYKVVAHS
                     TEQEWIECLVAFHGEDFRDHFEMLAAVEGPWFYYEIQTD"
     gene            5769..>6340
                     /locus_tag="VHF05_00390"
     CDS             5769..>6340
                     /locus_tag="VHF05_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVS79418.1"
                     /translation="MPKTTEAKRAAAEQALTPGSLPKKVFGRFKKPLVDVPYLVESQI
                     DSYNWLIEEGLKEVFSEFSSIKDYSEKKFQLDFTGFELAEPKYDEFYAKENKLSYEAP
                     LKVRIKLKNFAQKTEKEQEIFMADFPLMTPHGTFIIAGIERVIVPQLARSFGAFFTAA
                     EIRGRQYFGAKIIPSRGAWIEIESDPEGVL"
BASE COUNT         1491 a         1621 c         1758 g         1470 t
ORIGIN      
        1 tcgcaggtca atccgccgcc gttcatcatt ctcgacgaaa ccgacgccgc gctcgacgag
       61 gcgaactcaa agaagtacgg cgatatgatc aagaatcttt ccgacaagtc ccagcttatc
      121 gtcatcactc acaatcgcga gacgatgtcg cgcgcgggaa tcctctacgg cgtaacgatg
      181 ggcaggggag gcgtttcgac gcttttgtcg atcaagttcg aggaggcggt ggcggtggca
      241 aaataaagct ctctcggact cgcattttga aaagcacgga tatttttcta ctaaaaaact
      301 tcttactgaa gctgtacacc ttttcgctct cgaagactca agctgaaaag gtcttgcgca
      361 cgctacgctt gctcgcgtgc tcacctcgag ctcgcgccag tcgcgctcgc tagtcttttc
      421 aaaatgcgag tcctcgttca tgtgttctga aggagcaaat gcaaagggca taagggctgc
      481 gtttgcgcat taattaagat tttttccacc acataaatat tttgaatttc gaattgctcg
      541 ctccggcatt ctgtaggtat gaatctgcag agccagattc attcactatt agataaaatg
      601 aaagagccgg atcgggaatg ccgatacggc tctttggttg ggttatgcgc aaacggtaat
      661 ttgttcgcgc agctgcggag gaattttttg aaggacctct ctttttcgcc tccttacttc
      721 tcccagattg cgaagtccga aatggtccat aatttgtttt ggcggattgc ggtggactat
      781 atgcaggaat aactgccagt cggccggttg tctcaggtgt cttcgcgcct ttgtttttat
      841 ttctgtactg ttcactttcc ctccatactg tagaacttgt cgtccttgct cattttgcgg
      901 agcccatatg aaggtagaat gaaatgaaaa ttttgtccac cccatgccat aattctgcag
      961 aattatggca tgttgcaaat cattaaatta gacgaatttg aaatccagaa tcttgcggtc
     1021 gagatccgcc gctacgagct tcaccttgac cttgtcgccg agcgagaggc ggcggccggt
     1081 gcggcggccg acgagcgaat aggtcttctg gtcgagttcg aagaaatcgt cgccgatatc
     1141 cttgatgcgg accatgccct cggcatgtgt ttcgggatct tcgacataga cgccccattc
     1201 ggtaacgcca gagacgacgc cgtcgaagac ctctccgacg cgatccttca agaattcaac
     1261 ctgcttgtat ttgatgcttg cgcgctcggc atccgccgcg acgacttcct tctccgacga
     1321 ttccatggca atgcgctcgt atttggcgaa gtcttccgaa acgtgcgccg ggtcggtgag
     1381 atatttgaag aggagcctgt gcacgataag gtcgggatag cggcggatgg gggaggtgaa
     1441 gtgcgtgtaa ttcctgaatg cgaggccgaa gtggccgatg ttgcgggtcg agtagatcgc
     1501 cttcgccatg gagcggatgg cggcggtctt gatgagctcc tcctgcggca taccctcgat
     1561 cgattcgagg aggcgcgaga tatcatatgc ggtgacttcc ttgcccggcc tgccaaggtt
     1621 gaggccgagc gccttgagga agagcgtgag gttctcgatg cgatccttgt cgggcatgtt
     1681 gtggatgcgg aagaggaacg ggcgcttcgc ggctccatgc cctcccgccg ctttttcgag
     1741 cctcgtgatg tattcggcga cttcgcggtt cgaaaggagc atgaactcct cgacgagctt
     1801 gtgggtgtcg agtcgctcct tcttgatgat ctcgatgggc ttgccgtcct tgtcgagctt
     1861 gaatttgatc tcggtcgtat cgaaatcgat ggcaccggcc tttttgcgct cctcgccgag
     1921 cttcttggcg atggcattca accggcgaag tacgtctccg tgctcgccgt tttcatcgat
     1981 cgacttctgg gccgtttcgt acgagaagcg cttgtcggag cggatgaccg ttttgccgat
     2041 ccagcggtct tccacctgcc cctccgcgtt catgatgaag acggtcgaga aagcgagctt
     2101 gtcttcctcg gggttcagac tgcagaggtc gttcgagagg atttcgggga gcatggggat
     2161 cgtgcggtcg acgaggtaga cgctgaagcc gcgcttgcgc gcctcgcggt cgagctcggt
     2221 gccttcgcgg acataatgag aaacatccgc aatatgaatg ccgacttcga actttccgtc
     2281 cccgagctcc ttgaaggaga gcgcgtcgtc gaagtccttg gcgtcggtgg gatcgcaggt
     2341 aaaggtgaag gttccgcgca tgtcgcggcg gcccgcgatt tcgtcggggc ggatgggatg
     2401 ctcggtgcgg gcgagctcgt tcgcctcgtc atcgactgcg tgagggaagt gcgtatcgat
     2461 gccgcgctcg aggatgatcg actggatttc cgtctcgtgc attcctttgc ggccgatatc
     2521 ctggacgatc gcgccgatag ggttcgactt ggggctgtcc cagcgaacga ttttcacgag
     2581 aagcttttcg ccggaagaga ggcccttcgc ctcgtcggcg ggaatgagga tgtcgaggta
     2641 catcttgcgg tcgtcgggga tgaggtacgt tccctcaggc gccgcgtcga cggtgcccac
     2701 gaactccatt ttggcccgct cgatgatgcg gagcacgatg ccctgttcgc gaaggacgcc
     2761 atgggttttc ggaagaaggc cgatctcgac ggtatcgccg tggagcgcca tgttgagatt
     2821 ctcgggctgg atttcgatgt cctcctcgcg gccttcaatg gggaaatagc cgactccgcg
     2881 gccgttgacg cttatgattc ccgtgatgcc gggcttttgg gatgcggcag tctcaagagg
     2941 agaggaggca ggagccgata aggcagaggc gtcaggcgca ggaaaacggc gcggccgtag
     3001 aggcggacga ggaccgaact ttctctcaaa accgccattg ccgccatttc tcgaaaactg
     3061 cgatccattg aaatttccgc tatttccgcc gttcctgcga tcgcggtcat ttctccagtt
     3121 gccgccgcgc tcattgccgt tgcggccccg gccgccgttc ctgtttgtat ccataaaatt
     3181 gattctagca gaaaatccca aaaaagcttt attttggcaa cttcggccta tattccatgg
     3241 ctctgttgac tctataaaaa ccatctgtta gtattgtcgt catgttaaaa atcaggttac
     3301 agagaaccgg caggaagcac gaaccgacct tccgcctcgt cgttaccgag tcgcagaacg
     3361 cggcaaagag cggcaaggcg ctcgaggtct tgggttccta cgatgcccgc aagaccaatg
     3421 agataattca cacggaccgt gtgaagcatt ggatcgagaa gggagcgaag ccatccgata
     3481 cggtccataa tctcctcgtt tcgaagaaaa tcatcgaggg caggaaggtg aacgcactcc
     3541 cgaagaagaa gccgatcatt gatcaggcga aactcgacgc cgagaaggca gctgcagagg
     3601 cggcagaagc cgagaaggcg agggcagagg ccgaagcggc ggcagccgca gccgcggcgg
     3661 aagctcccgc tcccgcagaa gcgccggccg aagaagccgc tcctgcagaa gcgcccgcgg
     3721 aaactccttc cgaggaaact cctgctgaat agtagaaggt agcgagtaga taggactcag
     3781 actggaaaaa cttccgcagt gcggaagttt ttcttttctc tctacccact actctctact
     3841 caatacttct ctaattgctt ttatccctat gctctattaa aatgaaacaa gcagtacctc
     3901 acgcggctac ggcatcactg tagcaatgag tgggtccaaa actgcggata atcattaaca
     3961 gctgatcgaa cttatggaac accaagacac ccaatttcta gattttgtaa tcaaggcgct
     4021 ggtcgacaat ccggacaaag ttaagataaa ccgcacggta gatgaaatgg gcgtgctttt
     4081 gacgctcgat gtggacccca tagacatggg caaggtgatc ggccgctcgg gcaataccgc
     4141 caaggccatc aggacgcttc tccgcgtcgt tggcatgaag aacaactccc gcgtcaattt
     4201 gaaaatcaac gagccggaag gcggcatgcg caatgaatcc ggcaaggcga ccaagacggt
     4261 cgacgaggcg atggaggacc tctcgatata aaacagtaaa ttagtagtta gtagctagac
     4321 ttgaaaatag gcgccgagag gcgcctgttt tcaaaacaca aaaatcgtgg tattttgagt
     4381 cagttcgttt ccgcattttt ctaagtactg tctactaggt actatctact ttcccagtga
     4441 attttcacgt catcacactt tttcccggag ctttcgactc gtatcttggc gagtcgattt
     4501 tgaagcgcgc catcgaggac aaaaagatca aagtccattt ctacaatccg cgcgatttta
     4561 cgaaggacaa gtggcgccgc atcgaccgca agccctacgc gggcggcccc ggcatggtga
     4621 tcgaagcgct tccggtcatc aaggcgatag agaaagcgat gcaaaaatca aagatcaaaa
     4681 ataaaaaatc aaaaagaaat gcgaagattt tcttcttgag tcctggaggc aagcagttta
     4741 cgaacgagat aggggagaag gcagcgaaaa aatataccga tatcattatc gtgtccggca
     4801 ggtacgaggg aaccgacgcg cgcatcaaga aagtcttcaa gatggaggat atctcgatcg
     4861 gcccgttcgt ccttaccggc ggcgagcttc cggcaatgct catgatcgac gtgatcgcaa
     4921 ggcgcgtgcc gggagtgctc ggcaatatcg gcagcctcga agaatcccgc gtatcctccc
     4981 acgacgttta cacccgccca gaagtttttg agtacaaagg caaaaagtac cgcgtgccga
     5041 aaattctttt gggaggagat cacaagaaaa tagaggagtg gaagaaaaag agaaaataaa
     5101 tataatgtga gcaggaggtg ggcatggcaa gcacgaagac ggtgattatt tcttccattc
     5161 cggacctgga aattggcaaa atttatctcg gcttaaccaa aggcccctgg taccagccgt
     5221 tacctggcca gtgttacaaa gtagttgccc attctactga acaagaatgg atcgaatgcc
     5281 tcgtcgcctt tcatggagag gatttcagag accattttga gatgttggct gccgtagagg
     5341 gcccgtggtt ctactatgaa atacagaccg actaaggctc tctttcaaca aggagagcct
     5401 tttaaatacc agaaaactgg gtgccggctg ggcagattct acatactctc gtcgagggct
     5461 gggtactggg cacattcctt tcctgaggtg gggaaaagca cgttgacatg aaatttcaat
     5521 gggtatataa tctccccacg attgcaaaag attgtgttgc gggcgcaatc ccattaaaag
     5581 cttcgctggc cgtggccacg gctggagcaa ggaaactttt tgcatactct tagctaattc
     5641 aatgaactga aaaaaaagac cctccccacg aggcgtcttt tgatgtttgt atggatagcg
     5701 atgagctttt gccgggagtt tcggaaggag acggtcaatt taatagaaaa tctaccccac
     5761 gtagaaacat gccaaagaca accgaagcaa agcgggcggc ggccgaacag gcgctgaccc
     5821 cgggaagctt gccgaaaaag gttttcggcc gcttcaaaaa gccgcttgtc gatgtgccgt
     5881 accttgtcga atcgcagatc gattcgtaca actggctcat cgaggagggc cttaaggagg
     5941 ttttctccga gttctcgtcc atcaaggact actctgagaa gaagttccag ctcgatttca
     6001 ccggattcga gctcgcggaa cccaagtacg acgaatttta tgcaaaggag aacaagctct
     6061 cctatgaggc tcctttgaag gtgcgcatca agctcaagaa cttcgctcag aagacggaga
     6121 aggagcagga gatcttcatg gcggattttc cgctcatgac gccacatggc accttcatta
     6181 tcgccggcat tgagcgcgtc atcgttcccc agctcgcgcg ctcattcggc gcgttcttca
     6241 ctgcggctga aatccgcggc cgccagtact tcggcgcgaa gattattcct tcgcgcggtg
     6301 cctggatcga gatcgaatcc gacccggaag gcgtcctcta
//
LOCUS       DASZYS010000006         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_649267, whole genome shotgun sequence.
ACCESSION   DASZYS010000006 DASZYS010000000
VERSION     DASZYS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649267"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VHF05_00395"
     CDS             complement(<1..689)
                     /locus_tag="VHF05_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVS79419.1"
                     /translation="MEDYLKDLNPEQREAVEITQGPLLIIAGAGAGKTKTLTYRILHL
                     IQTGVAPSNILAITFTNKAAKEMRERTLSLLRTNRGSGLSLAWDERPFVSTFHALGVH
                     ILKENAARLGLPRHFGIFDKSDGKRAVKDALEKMGLDPKQYEPGKIQSVISREKGNFV
                     TYEEYAEAAGGDFFSNIVAKAWGHYEKTLREEKALDFDDLLLRTAILLRDNEEVREHY
                     ADTWKYVHIDE"
     gene            1203..2315
                     /locus_tag="VHF05_00400"
     CDS             1203..2315
                     /locus_tag="VHF05_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVS79420.1"
                     /translation="MARTSVRSMAAFALAAAVLVPTVARAGMFSFVSGLFQDGGEETP
                     AVIPNNSQNMAVLAASPSPNQSEGGADVAIVDGTTLAAHDGPQSSEDRDTPHRPGSDQ
                     ISVYVVRDGDTLSGIAQMFDVTVNTILWTNDLDSKTVRPGQVLIILPVSGVQYTVKKG
                     DTIASIAKATGGDATEIANYNEIDDNSQLAAGTTLIIPNGEAPAVASVPSTAKKSGSS
                     SSSSSSSSKKSSTPLIASEIGGTHAAPMAAASGATGDTTGYYMRPLKGGIKTQGIHGY
                     NAVDIGTPVGTTIYAAASGDVIVSKDSGWNGGYGNYVVISHPNGTQTLYAHLSSTIVV
                     AGQHVVQGQVIGYSGSTGESTGPHLHFEVRGAPNPF"
     gene            2443..4077
                     /locus_tag="VHF05_00405"
     CDS             2443..4077
                     /locus_tag="VHF05_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HVS79421.1"
                     /translation="MKALFSRLVSGIERTPISAWEWLFGAAGILFVRFLLENFTTASA
                     SGIIASDAPTLVHYLLTFFACALALAVVGAALAPEKRDAWLKISLFGLLLTWISPLID
                     LISREQMAYLFDVPDNLLRDFLTFFGPLVSRGVSTGLRFEIALILIILFIFLRWYAGK
                     SWAKSLFGAVLGYAALFIIGSMPSLIYALHVSVSPSVPLLFSPNPIADYLSNTFSHSL
                     IAKNFLHPTELLDSSVRYFEIYFDAAMGCIMYILCVSLAALYVFLAWPEKIKSVLKNS
                     RPERVAHYFILIVAGIVIAAAEYRVSWRGWPDILSILILFFSFYAAWMYAAGRNDIED
                     IATDRISNPERPLVAGSLSISEMESFNAFFLVAALLGGFLAGYYALYMVLAYILLSHI
                     YSCRPLRLKRFPVVSSFVISLASLAALMAGYFFWGTNPVISSFHVSWAVLVVIFFTLA
                     ANIKDIKDFEGDKMSGVHTIPVMFGAEKGRRIIALCCMLALLAVPLLLDRAILFAASI
                     PTAVFAWIVMTRDPFREKNVFALYFLFFALVAVILI"
     gene            complement(4110..4658)
                     /locus_tag="VHF05_00410"
     CDS             complement(4110..4658)
                     /locus_tag="VHF05_00410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79422.1"
                     /translation="MTHKEIVSLIEGAVLREDDGAGIAWADLGSGEGAFTLGLRELLG
                     PRAWIFSVDKNKRRLAVQKEAFDANFPGSPTEFIHADLAGDKWREGLPDLDGILMANT
                     LHFFGNQEEFLATLRTLLKPHGRLVIVEYDTDRKSLFVPHPLTFETWQTLAERSGFGA
                     AKLIARAKSKYWGSMYGALALR"
     gene            complement(4847..6424)
                     /locus_tag="VHF05_00415"
     CDS             complement(4847..6424)
                     /locus_tag="VHF05_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HVS79423.1"
                     /translation="MGFSKVYSAQTNLLKAHIIDIEVDLSNGLHSFSIVGLPDKAVEE
                     SRDRISAAIKNSGFESPKSKNQKIVISLAPADVKKEGPWFDLPIAVSYLKAAGEIAFD
                     TGDKIFLGELSLDGDVRKLSGALALAQGAKAAGFKEAYVPKENAEEAALVEGLTIYGV
                     ENLRELVEHLKALQWEKHGKKEGEKEPFRKIAPFEPVEETGIETTPPLVDLSDVKGQE
                     TAKRGLEIAAAGGHNIAMYGPPGTGKTMLAKAFAGLLPELPFDDALEVTAIHSVAGTL
                     KETLVRRPPFRSPHHTSSYVSLVGGGANLKPGELTLAHKGVLFLDEFPEFDIRTIEAL
                     RQPLEEGVVSVSRAKGTAHFPANVILVAAMNPCPCGYLGFKGRECTCTPAQLIRYKRK
                     LSGPIMDRIDLWVEVGNVPHAELISETKGESTAAVGERVKKARDIQAERFKKLNKSIS
                     TNSEMGAKEVVLFVKLNKDTQEIMNRAAFRYGLSARSYHRILKVARTIADLAGSEDIA
                     SDHILEAFQYRPKKQEI"
     gene            complement(6435..6797)
                     /locus_tag="VHF05_00420"
     CDS             complement(6435..6797)
                     /locus_tag="VHF05_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVS79424.1"
                     /translation="MNTDFSEQKKSLEAELESLTKELKSVGRVNPDNPLDWEAVPEAI
                     DTDSADSNNTSDKIDQYEENTAILKQLEARYNSVKDALARIDAGTYGTCKVCGKDIES
                     ERLAANPSATTCEEHMNS"
     gene            complement(6860..>7597)
                     /locus_tag="VHF05_00425"
     CDS             complement(6860..>7597)
                     /locus_tag="VHF05_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79425.1"
                     /translation="VSLVLSILLVLVGASIFIVSQFFASPSQEPVIVYDTLMPYDETE
                     KIDLTEMTREKIVSVFAEERSQAVPSGQIKYLKLVESGAAVEQDATISRFLALVEAKA
                     PAEFVRSLDGTFMAGIYGDGQASDPFLLIKLSSFDNAYSGMLTWESTLWNDLGPLMSA
                     DRAAPVVKDTGFAVTGTTTAFVTAPSQSDFDDLIVNNLNTRVVRNDAGQIIFLYSFID
                     NDTLLITTNDLAFQGITSKYTASKLIR"
BASE COUNT         1463 a         2171 c         2098 g         1865 t
ORIGIN      
        1 tactcgtcga tgtggacgta cttccatgtg tctgcgtagt gctcccgtac ttcctcgttg
       61 tcgcggagga gaatcgccgt gcgcagaaga aggtcgtcga agtcgagcgc tttctcttcg
      121 cgcaaggttt tctcgtagtg cccccatgcc ttggcgacta tgttcgagaa gaagtcgccg
      181 ccggcggcct ccgcatactc ttcatatgtc acgaaattcc ccttctcgcg ggagattacc
      241 gactgaatct tgccgggctc gtactgcttg ggatcgaggc ccatcttctc gagcgcgtcc
      301 ttcacggcgc gcttgccgtc gctcttgtcg aagatgccga aatggcgcgg gagcccgagg
      361 cgggccgcgt tttccttgag aatatggacg ccgagcgcgt ggaaagtcga aacgaacggc
      421 cgttcgtccc aggcgagcga aaggcccgag cctcggttcg tgcggaggag ggagagggtg
      481 cgctctcgca tctccttggc cgccttgttg gtgaaggtga tagccaggat attcgagggt
      541 gcgacgccgg tctggataag atggagaatg cggtaggtca gggtcttggt ctttcccgct
      601 cccgctcccg caattataag gaggggtcct tgggtgattt caacagcctc gcgctgttcg
      661 gggttgaggt ctttcaaata gtcttccatc gggactacta taacatagcg gctctccgat
      721 catattaaag ccatggattt cgtgggcgaa gctcgccttt ttggggaaga atgtggcggt
      781 acgataaaat tatcaattta taatttctaa ttttcaatca agccacaatg cttcaattcc
      841 tgcttagtgc gggcagattc agacgtttga aatattttaa aattgaaaat tcattgaaaa
      901 ttggaaattg agcgttaaaa atttccttca aacttcatgc gaggggacaa gtgcaatgga
      961 atttctgcga ttttcttact taagcagtga aattatgcaa cattttttcg ttgactcaaa
     1021 aatgtgagaa cgtataatat taagtgttaa tgaaggactg aactaccgtc atatggctta
     1081 attgagccat tttaccgaac tctggaatta ccattaattt aaagcttatc gttccacatg
     1141 gacagaatcc cggcgtagag gatggggaaa aggattccgg ccgggatact ctcaaaaggc
     1201 tcatggcccg cacctcggtg cgatctatgg ccgcgtttgc gcttgcggcg gccgttctcg
     1261 tgcctactgt ggcgcgagcc ggcatgtttt ctttcgtttc ggggcttttt caggacggcg
     1321 gagaagagac tcccgcggtg attcctaata attcccaaaa tatggcggtt cttgccgcat
     1381 ccccttcccc caaccaatcg gaaggcggcg ccgacgtagc gatcgtcgac ggcactaccc
     1441 ttgccgccca tgacggcccg cagagctccg aggatcgcga tacgccgcac aggcccggaa
     1501 gcgaccagat cagcgtctat gtcgtccgcg acggcgatac cctctcggga atcgcccaga
     1561 tgttcgatgt gaccgtgaat accattctct ggaccaacga tctcgacagc aagacggtcc
     1621 gtccgggcca ggtgctcatt attctccccg tttcgggcgt ccagtatacc gtgaagaagg
     1681 gcgatacgat cgcgagcatc gccaaggcga ctggcggcga tgcgactgaa atcgccaatt
     1741 acaacgagat cgatgacaac tcccagctcg ccgcgggcac cacgctcatc attccgaacg
     1801 gcgaagctcc ggcggtagcc tcggtgccgt cgaccgcgaa gaagagcggc tcctcatcct
     1861 cttcctcaag ctcttcaagc aagaaatcgt cgactcccct catcgcctcg gaaatcggcg
     1921 gaacgcacgc ggcgccgatg gccgccgcct ccggcgcgac cggcgacacg accggctact
     1981 acatgaggcc gcttaaggga ggcataaaga cgcagggcat ccacggctac aatgccgtcg
     2041 atatcggaac gcctgtcggt acgacgatct atgcggccgc atcaggcgac gtgattgtga
     2101 gcaaggattc cggctggaac ggcggctacg gtaattacgt cgtcatctcc catccgaacg
     2161 gcactcaaac cctctatgcc cacctttcga gcactatcgt cgtggcgggc cagcatgtcg
     2221 tccagggcca ggtgatcggc tactcgggct ccaccggcga atcgaccgga ccgcatcttc
     2281 attttgaggt acgtggcgcg ccaaatccat tctaaattcg aaaaacccgc gcttccggcg
     2341 cgggtttttc attgtcggat atgccatttg gcatttggaa aataacgagt attatcagtt
     2401 catcagaaac gactgccgca ctgtatttcc caatcagttt ccatgaaagc attattttcc
     2461 aggctggttt cgggcataga acgcacgccg atctccgcgt gggaatggct tttcggcgcc
     2521 gcgggcatcc tcttcgtacg cttccttctg gagaatttca ctaccgcctc ggcaagcggc
     2581 attatcgctt ccgacgcgcc gacgctcgtc cactatctcc tcactttttt cgcctgcgcg
     2641 ctcgcactcg ccgtcgtagg cgcggcgctc gctcccgaaa aacgcgatgc ttggctcaag
     2701 atttccctct tcgggctcct tctcacgtgg atctcgcctc tcatcgatct tataagccgc
     2761 gaacagatgg cgtatctctt cgatgtgccc gacaatcttc tccgcgattt tctgactttc
     2821 ttcggcccgc tcgtttcccg cggcgtctca acgggactcc gcttcgagat cgcgctcatt
     2881 ctcattatcc tcttcatatt tctccgatgg tatgcgggaa aaagctgggc gaaatccctc
     2941 ttcggcgcag tgctgggata tgccgcgctt ttcattatcg gctcgatgcc cagcctcata
     3001 tacgcactgc acgtttccgt ctcgccctcc gtgcctctcc tgttttctcc gaacccgatc
     3061 gcggattatc tttcaaacac cttctcgcat tcgctcatcg caaagaattt cctccatccc
     3121 acggagcttc ttgattcgtc ggtccgttat ttcgaaattt actttgatgc cgcgatgggc
     3181 tgcatcatgt acatcctctg cgtctctctc gcggcgctct atgtattcct cgcatggcct
     3241 gaaaaaatca aaagcgtatt aaaaaactcg cgtcccgaac gcgtggcgca ctattttatc
     3301 ctcattgtcg ccggaatcgt cattgccgct gcggagtatc gcgtatcctg gcgcggctgg
     3361 cctgacattc tctcgatcct catcctgttc ttttcgttct atgcggcctg gatgtacgcc
     3421 gcgggccgga acgatatcga ggatatcgcg accgaccgca tctccaatcc cgagaggccg
     3481 cttgtcgcgg ggtcgctttc tatttccgaa atggaatcgt tcaacgcctt ctttcttgtg
     3541 gcggcgctcc tcggcgggtt tctcgcgggc tactatgccc tctatatggt gctcgcctac
     3601 attctcctct cgcatattta ttcctgccgc ccgctccggc tcaagcgctt tcccgtcgtg
     3661 tcgtcatttg tcatttccct cgcgagcctc gcggctctga tggccggcta tttcttctgg
     3721 ggaacgaatc cggtcatttc ttcctttcac gtatcatggg ccgtactcgt cgtcatcttc
     3781 ttcacgcttg cggcgaatat caaggacatc aaggatttcg agggcgacaa gatgtcgggc
     3841 gtgcacacga ttcccgtgat gttcggcgcg gagaaaggta ggcgcatcat cgccctctgc
     3901 tgcatgctcg cgcttctcgc cgtaccgctt cttcttgatc gcgcgattct ctttgccgca
     3961 tcgatcccga ctgctgtatt tgcctggatc gtcatgacgc gcgatccttt ccgcgagaag
     4021 aacgtgtttg ccctctattt cctcttcttc gctctcgtcg cggtcattct tatttgaacg
     4081 aaataaatcc ggccgaagcc ggtttttcat catcggagcg cgagggctcc gtacatgctg
     4141 ccccaatatt tcgacttggc gcgggcgata agcttggccg ctccgaatcc gcttctctcc
     4201 gcaagcgtct gccacgtttc gaacgtgagc ggatgcggca cgaagaggct cttgcggtcg
     4261 gtatcgtact cgacgatgac aagccggccg tgcggcttga ggagcgtgcg gagggttgca
     4321 agaaactcct cctggttgcc gaaaaaatgg agcgtgtttg ccatgagaat gccgtcgagg
     4381 tccggaagcc cttccctcca tttatctccc gcgagatccg catggatgaa ttcggtgggg
     4441 cttccgggaa agttcgcgtc gaacgcctct ttctgcacgg cgagacgccg cttgttctta
     4501 tcgactgaga atatccaggc gcgcgggcca aggagttcgc gcagccctag ggtgaacgct
     4561 ccttctcccg aacccaaatc cgcccatgcg atgcctgcgc cgtcatcttc cctcagtacg
     4621 gcgccctcga tcaatgaaac gatctctttg tgtgtcacta gaacatcctc gcaatctaag
     4681 atgaagaaaa attaaaagtg agataggccc gaagctttta gcttgttggg aatgccaagc
     4741 agaaaaaaca atccataatg tgagagtcaa actctcacat tctatactga ttctgcatgt
     4801 ctcatctttc taaaagctaa cagctataag ctttcttgct gttttttcag atttcctgct
     4861 tcttcgggcg gtactgaaag gcttcgagaa tgtggtcgct cgcgatgtcc tccgacccgg
     4921 cgaggtcggc gatggttctg gcgactttga gaatcctgtg gtaggagcgg gccgagaggc
     4981 cataccggaa ggcggcccga ttcatgattt cctgcgtgtc cttgttgagc ttcacgaaaa
     5041 gcacgacttc cttggcgccc atttcgctgt tcgtcgaaat gctcttgttg agtttcttga
     5101 atcgttccgc ctggatgtcg cgcgctttct tcacgcgctc gcccactgcg gccgtgcttt
     5161 cgcctttcgt ttcggaaatg agctcggcat gcgggacgtt gccgacttcg acccagagat
     5221 cgatgcggtc catgatgggg ccggagagct tccgcttgta gcggatgagt tgggcaggcg
     5281 tgcaggtgca ctcccttccc ttgaagccga gatatccgca ggggcatggg ttcatggcgg
     5341 cgacgaggat tacgttcgcc ggaaaatgcg cggtgccttt ggcgcgggaa acggagacga
     5401 cgccttcctc gagcggctgg cgcaaggctt cgatggtgcg gatatcgaac tcgggaaatt
     5461 cgtcgagaaa gagaacgcct ttgtgcgcga gagtgagctc gccgggcttc agattcgcgc
     5521 cgccgccgac aagtgaaaca tacgacgacg tatgatgcgg cgacctgaac ggcggacggc
     5581 gcacgagcgt ctctttgagc gtgccggcga ccgagtggat cgcggtcact tcgagtgcat
     5641 catcgaacgg taattccgga agaagccccg cgaacgcttt ggcgagcatg gtctttcccg
     5701 ttccgggcgg cccgtacatc gcgatgttgt gcccgcccgc cgcggcaatt tcaaggccgc
     5761 gcttggcggt ttcctgaccc ttcacgtcag agaggtcgac gagcggcggc gttgtttcta
     5821 tcccagtttc ctctacgggc tcgaacggcg cgatcttcct aaacggctct ttttcgcctt
     5881 ccttcttgcc gtgtttttcc cactggaggg ctttcaagtg ctcgacaagc tcgcggagat
     5941 tttctacgcc gtagatggtg agaccctcga caagcgcagc ttcttccgcg ttttctttcg
     6001 gtacgtacgc ttccttaaac cccgccgctt ttgctccctg cgcgagcgcg agcgcgccgc
     6061 tcagcttccg cacgtcgccg tcgagcgaca attcgcccag gaatatcttg tcgcccgtgt
     6121 cgaatgcgat ttcccccgcc gccttgagat agctcacggc aatgggcaga tcgaaccacg
     6181 gcccttcctt cttcacgtcc gcgggcgcga gcgagatgac aatcttctga ttcttcgatt
     6241 tcggcgattc gaagccggag ttcttgatgg ccgcggagat gcggtcgcgc gattcctcga
     6301 ccgccttgtc gggaagcccg acgatcgaaa aggagtggag tccgttggag agatccactt
     6361 cgatgtcgat gatgtgtgct ttcaggagat tcgtttgggc gctgtaaact ttggaaaagc
     6421 ccatgggatt tggtctagga gttcatgtgc tcctcgcacg tggtcgctga agggtttgcc
     6481 gcgagccgct ccgattcgat gtccttgccg cagactttgc atgtgccgta cgtgccggcg
     6541 tcgatgcgcg cgagcgcgtc cttgactgag ttgtagcggg cctcgagctg ctttaagatc
     6601 gcggtattct cctcgtattg gtcgatcttg tcggacgtgt tgttgctgtc cgcgctgtcc
     6661 gtgtcgatcg cttcgggaac cgcctcccaa tcgagcggat tgtcgggatt gacccggccg
     6721 acgcttttca attccttcgt aagggactcc aactccgcct caaggctctt tttctgttct
     6781 gagaagtcag tattcatagt gaattaatat ggtgaattaa tgctatcaaa attaggcctg
     6841 ggtgcaaagg aaggcgtcct taccggataa gcttcgacgc ggtgtatttg ctcgttatcc
     6901 cctggaaggc gagatcgttc gtggtgatga gaagcgtatc gttgtcgatg aaggaataga
     6961 ggaagatgat ctggccggcg tcgttccgca ccacgcgggt attcagattg ttgacgatca
     7021 gatcgtcgaa gtcggattgc gacggcgcgg tgacgaatgc ggtagtggtg cccgttaccg
     7081 cgaaaccggt gtctttgacg acgggcgccg cgcggtcggc gctcatgaga gggccgagat
     7141 cgttccagag ggtcgattcc caggtgagca tgccgctgta cgcgttgtcg aaagacgaga
     7201 gcttgatgag gaggaacggg tccgaggcct ggccgtcgcc atagattccg gccatgaacg
     7261 ttccatcgag cgagcgcacg aattcggcgg gcgccttcgc ctcgacaagc gcgaggaaac
     7321 gcgagatcgt cgcgtcctgc tctaccgccg cccccgactc gacgagcttc agatatttta
     7381 tctgtcccga aggaacggcc tggctgcgct cctccgcgaa tacggagacg atcttttcgc
     7441 gcgtcatctc cgtgagatcg atcttctcgg tctcatcgta gggcatgagc gtatcgtaaa
     7501 cgatcaccgg ctcctgcgag ggcgatgcga agaattgcga gacgatgaaa atcgacgcgc
     7561 cgacgagcac gagaagaatg ctcaagacga ggcttac
//
LOCUS       DASZYS010000007        23485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_777299, whole genome shotgun sequence.
ACCESSION   DASZYS010000007 DASZYS010000000
VERSION     DASZYS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 23485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23485
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777299"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..455
                     /locus_tag="VHF05_00430"
     CDS             201..455
                     /locus_tag="VHF05_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79426.1"
                     /translation="MEYKGELALEEDLSFEEYYAAIDDMDDSDLNPGEEDEEEGENGE
                     DDDKDEEDEEDDDESEETDEEEPTDEDLERLEEEGSDESA"
     gene            668..1885
                     /locus_tag="VHF05_00435"
     CDS             668..1885
                     /locus_tag="VHF05_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVS79427.1"
                     /translation="MFGWEFPPYNSGGLGVACLGLARALLRAGLEVSFVLPKRLGAVN
                     SAVRVVFGDDYYRELGLDTITLHEVDSPLYPYLTSSEYEAAGRSGLKTSPYAKTLIGE
                     VLRYAMTAAKVARDEEFDVIHAHDWLSFPAGLEAKRISGKPLIVHVHATEFDRGGGTG
                     VNQEVYDIERKGMEGADKVIAVSHHTKDILVSRYSIPPEKISVVHNGIDEADYLVPHG
                     TPSEIQALRAAGNKIVLFVGRITIQKGPDYFVKMARKVLEYKKDVYFVVAGSGDMEWR
                     MVEAVAAAGMSDRFIFTGFLRGEELNEVYRAADLYVMPSVSEPFGITPLEANLNGTPV
                     LISKQTGAGEILSHALRCDFWDTDEMANQALAVLEHRSLHDTLRENGRAETRSIDWNK
                     AAEKCIIVYDSVA"
     gene            1960..2550
                     /locus_tag="VHF05_00440"
     CDS             1960..2550
                     /locus_tag="VHF05_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79428.1"
                     /translation="MEKPASRREREKDDKRGNSHRQLDIIERKWGLLYGFRYSVIAVA
                     VVSQRILWGRFISLYHEAVNLKQNCFGSLSLQEAAAKFKECSLENLISIDLRLVGVVL
                     VAIVFTIAIMIEENHLYKQSEVCADAGIEKEKSLNIPDGIYIRLRQLEMSKQARFLRI
                     IHMRSVFALLLFVALCLWIGGLILVVDQFIVPWYNF"
     gene            2868..4121
                     /locus_tag="VHF05_00445"
     CDS             2868..4121
                     /locus_tag="VHF05_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HVS79429.1"
                     /translation="MSSVCFYFQVHQPHRLKRYRIFDIGKDRNYFDSADPRLSNEAIL
                     RKVAGKCYLPANRLLLDLIRKYPKFKVSFSISGTALEQFEKFAPDVLQSFVDLVETGN
                     VEILSETYYHSLAFLYSPEEFRNQVELHKKLVKRIFGVTPQVFRNTELIYNNDIAREV
                     ERMGYAGIIAEGADRILGWRSPNFLYRPLIPDRAHNLKLLLKNYRLSDDIAFRFGNKD
                     WEGYPLTAPKFGQWVSAHNGNGEVLNLFMDYETFGEHQWESTGIFDFLRALPEEVLRN
                     PDNDFVTPSEAVSRYRSVAELDIPDYVSWADIERDLSAWLSNDMQREALKNVYELEEK
                     VRAAADEKLMEDWRRLLTSDHFYYMCTKWFADGDVHKYFNPYESPYDAFISYMNVLKD
                     MRARIEERERSAEYRIPIRKLAIAE"
     gene            4145..5428
                     /locus_tag="VHF05_00450"
     CDS             4145..5428
                     /locus_tag="VHF05_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79430.1"
                     /translation="MLFKRNGTAKQGPKRSLMERLVIVDANDVDPEEVAEAAAEVAGG
                     ENEAPEIGVVSFRAPRGEIHNELISSESQEGRRRKSFNPRMSFDAGSANLHTISEQEL
                     EDAVAQAIDREAQKIGVKHFEEPAVSEDDDFEPRITVSDPSEFAAPYEEPAELRREEP
                     EPAAEAYVIPIAEESRPIETDETETYEERPISIPIGKTFDILDRAKERPDEDERIIKI
                     VYRAPKVVSGLYAQPESKRQPLVFSEAMSPIAEEESAQAEESVESLIDAAIAREAEDA
                     LAEAEEPAAEAPVGRPDNLQDDAEIVARVLDENTSPITHEEAKAAENDTAPASWVSSV
                     EEAAMPKISHFDKVLFPEEYFYASNGDTIKTARQLYDALGNMDDATFAHHAIARNTND
                     FANWIKYVYGHEDVARQLMTARERTHMRQILEDYL"
     gene            5527..5907
                     /locus_tag="VHF05_00455"
     CDS             5527..5907
                     /locus_tag="VHF05_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79431.1"
                     /translation="MKVLIFNDEQKKLDEVKGVLDHVLKGYSVEITFAHVGNSFEANR
                     ACGRGEFDIIFADHFLDKLEPSNNAFRIVLRAQHYGARVFTTTEDTDVASKEYLPKGI
                     PHVPLVGHLIAEKILELLDARQFK"
     gene            6082..8034
                     /locus_tag="VHF05_00460"
     CDS             6082..8034
                     /locus_tag="VHF05_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVS79432.1"
                     /translation="MLVCLDEFAQVRDFYYPYVGQEDHLGRDLVHRVGVWVDGKISWL
                     SDGTWNISVKYANETMASDITCSNDGLGVELHFLDVVYNETNIFLRQVRIRNTRAAAR
                     HAKVFFNQEFRISENEHADTAYYNPTVEALIHYKGRRVFLVGGTIDGRPFDEYSVGLL
                     GIEGKEGTWKDAEDGVLSKNPIEHGAVDSVAGFSMALEPLASAEFSYWVCAGQTYREV
                     QALQKYIIAKTPAHLLETTQDFWHAWVNKISFTFQDLAQETKRLFKVSLLVIRAHCDN
                     RGGILASGDSGNFQQYGRDTYSYVWPRDGAFTALSLDKAGYTDIAERFFSFCNDLVTD
                     EGYLMHKYQPDKSLGSSWHPWVKDGKAQLAIQEDETATLIFAFWEHYRDMRDLEFVEK
                     IYNNFVKLCAEFMLSFREPQTGLPAASYDLWEEKWGVSAYTAASVYGALAAAANFANL
                     LGKEEEKLRYAREAERIRNLIIEYFLDKDTGGWRKLLNIVDGKAVYDTTIDASSFYGA
                     FRFGVLPAADPRMITSHKVLKDKLSSGEPFYGLGRYVGDKYFAAPENELGNPWFVTTL
                     WSVEYRINLALTRDDLQALAAELNRIAGFATSSGILSEQIDPATGKQLSISPLVWSHS
                     AFVSAVIAYMERAETLGLGKAVSDAS"
     gene            8034..10775
                     /locus_tag="VHF05_00465"
     CDS             8034..10775
                     /locus_tag="VHF05_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVS79433.1"
                     /translation="MQSRESQQKPQEEAKPVSWHAISVTESFSILSAGDRGLASEDAE
                     RRLLEHGRNVLPEEPVPGWGMLFLKQFMSPFVYMLLAASFIVYALGQTDDAVIILGVL
                     ILNACVGMIQEGRARDTLAALHDFTTSETTVLRDGREEIVSDEIVVPGDILVLREGDK
                     VPADARVISSQGLSADESALTGESTPVGKTAEALADQKLPVAERANMVFKGTLVVAGE
                     GVALVVATGVDTVIGSITKKIVEYDADVPLKEDIAKLSKSITWSVLAACAAVFALGVV
                     RGESVSEMFFVAVAVAVSLIPEGLPIIMTLVLALGVFRMGKRNALVKRLQAVEALGQA
                     TVIAVDKTGTITKNELMVSRLYVGGKDFGVTGDGYEPKGGIFLDDKEIVVGNNGELLL
                     AGKVAALCANATVAFEETEKLWKVTGDPTEAALRVFGEKCGFQKEGLDAEAPLVFEIP
                     FDSKHKYHATLHTIGGKLLLAVVGAPELILQAADSYWEHGAARPMTERAREDFRLRIE
                     EMSSKGLRVVGFAINEDAPSEISIDSMPPLTFAGLFGLEDVLREGVREAVETAQRKGV
                     KVVLISGDYRTTVTALAREAGIWKPGDMVVDGADIDRMSALSRSMLTVPTVFARVTPE
                     HKLMIIEAYKKHGDIIAMTGDGVNDALSLVDADLGIAMGRIGTAVAKEAADIILLDDN
                     FSSIIAALEEGRGIYRAIERVLIYLFSTGLGELFVVIGSLIFALPLPLLPSQILWLNV
                     VTDGLPVLALAFEPRMRGDGKPIAKDQNFIDSRSLVRTTIMGVVMAAGTLALFALGAG
                     VDLAHATTIALTALAVYQWFNIYNCRSNDASVTETKIFSNKSLNIAMIITVLLHCAAL
                     YAPFMQRILHTVPLSFTDWISVAFVALSVIAAEEIRKLVVRRKRERHAASEPVVSGV"
     gene            10786..11142
                     /locus_tag="VHF05_00470"
     CDS             10786..11142
                     /locus_tag="VHF05_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVS79434.1"
                     /translation="MKLIVKWLFTALAVLVAARFVPGIGVASLGTALLVALILGLVNA
                     IIRPIIVLFTLPVTILTLGLFILIINGVLFWLVASLVSGFEVAGFVPAFWGALIVSVV
                     SFIGNRFLHAVERNDE"
     gene            11270..12142
                     /locus_tag="VHF05_00475"
     CDS             11270..12142
                     /locus_tag="VHF05_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HVS79435.1"
                     /translation="MKIITHNGSFHSDDVFAVATLFLKFGKDHELIRTRDPEIIKSAD
                     IAVDVGHEYDESRLRFDHHQGPPLRPDGIPYASFGLVWKKYGAEIAGSSDAAVIIEHE
                     LVRPIDAADNSIDVVVPAIEGLFPYLIQDVVKALRPTWQEDADFDAGFMQAVGIADTV
                     LRREIMHARAKLEAQAKLQSFYDAAADKRMLILDRHYPKDDFLFAHPDVLFVIFPNDT
                     GEPSWIVRTVAAKEGAFESRKDLPESWAGLRDADLAKVTGVPDAVFCHRARFLAVAKT
                     REGAIKLAELALTA"
     gene            12205..13173
                     /gene="obgE"
                     /locus_tag="VHF05_00480"
     CDS             12205..13173
                     /gene="obgE"
                     /locus_tag="VHF05_00480"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVS79436.1"
                     /translation="MFVDELTIHMRAGKGGDGVVSWHHEHSKEYGGPDGGNGGKGGDV
                     YAEAVRDIGILAKERFEKEFAAEDGENGRGWSQQGASGEDIYVKLPVGSVVTNLSTGQ
                     QIELLSEGEKILVLKGGRGGLGNEHFKASTNVTPKEWTPGKEGEEADFHVELRLVADV
                     GFVGLPNAGKSSLLNALTRAQAKVGSYQFTTLEPNLGEMHGFILADIPGLIEGASEGK
                     GLGDKFLRHITRTRTILHCISVENEDVASVYDTIRTELKKHGEGLDEKEELIVLTKTD
                     ETTPEDLEKKIKILKKKNPRVTSVTILDDGSVTAFKNELIKLLQKK"
     gene            complement(13206..13469)
                     /locus_tag="VHF05_00485"
     CDS             complement(13206..13469)
                     /locus_tag="VHF05_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79437.1"
                     /translation="MTFIEFTIAPDSIGSSKGKRHYFVGKDEASILADTEKGREFGFA
                     KFESYRIVTEEYVRASIEEKNLPFLEDGINAVQNGTSDSYSIQ"
     gene            13569..14972
                     /gene="gatB"
                     /locus_tag="VHF05_00490"
     CDS             13569..14972
                     /gene="gatB"
                     /locus_tag="VHF05_00490"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVS79438.1"
                     /translation="MPYKLTVGLEIHAELKTSTKMFCDSKNDPDEERPNVNICPVCMG
                     HPGTLPVINREAVRHVLRMGTAVGGKIADFTEFDRKNYFYPDIPKDYQISQYKYPLVQ
                     GGSIAGFELTRIHLEEDTASSHHEAGDYSLVNYNRAGVPLMELVTEPVIHSAADAARF
                     AKELQLLLRYLGAGDANMEKGEMRVEANISVSASDVLGTKVEVKNLNSFRSVEKAIEF
                     EYKRHVAALEKGEELVQETRGWDEAKQVTFSQRKKESSHDYRYFPDPDLPKLKISEIP
                     EFSHNALRGSLPELPQAKRERLVASGLKESQAEVLTANFELGAYYDEVMNGSTVLAAA
                     AEQVANYVTSDLIGLLGGEGAALPGATNFRKLMELKAEGKIPTRVTKDLLGFLVTEDK
                     DPEILMEEKGIKAGDENELKQIVAKIVAENQKAVDEYKAGKEASLQFLLGQGMRATKG
                     TADPGTLKNLILEEMKK"
     gene            15121..16194
                     /locus_tag="VHF05_00495"
     CDS             15121..16194
                     /locus_tag="VHF05_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS79439.1"
                     /translation="MITGGPCAGKSTLLANARCYFENRGLRALVLTETVTELSLGGLP
                     PQEQWRTPISFQRMALGFQLSRENEYYRIAQELSVPKKTILLCDRGALDGMAYIKRHE
                     FLRVCEELECSPADLLDRYAGIIHLVTAAEGKEEYYTLANNSARTESAEEARRIDKRL
                     LAAWAGHERKAVIGNSTGFNEKMDRGLRAAARFANMPGFADCERKFILRNFAPWFIPR
                     SAVKIHIEQAYLALEGEVERRVRARRIDGATSYYYTELRPTPESGVRQKVTSLIPRRE
                     YEILLDEGRGVYPTVSKERYCFPYEGKQFEVDQFLKPQGVPILFEVKMETMDEPIRFP
                     SHWDANEVTGNAEFSNRNIALRS"
     gene            complement(16435..16680)
                     /locus_tag="VHF05_00500"
     CDS             complement(16435..16680)
                     /locus_tag="VHF05_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVS79440.1"
                     /translation="MSDAYVTIKQAAEILGVSPLTLRNWDKSGKFPARRHPMNNYRVY
                     RLSDLESVFDGIETGDASINSREKVHKLIVKHLENSD"
     gene            17097..18257
                     /locus_tag="VHF05_00505"
     CDS             17097..18257
                     /locus_tag="VHF05_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79441.1"
                     /translation="MNAEFFIDNKKYISAKRAAKLTGYATDYIGQLARNGKIDSKMIG
                     RSWFVNEESLLGYKKDLLDAMFATENAPLKKTKKRRSARKSAAPKNTPFTAEESAPKL
                     LPAGSSRIPELPGVRFISIAELEIELQTKAPFRMAAVPEESLPVQASDTPVEAPAPVP
                     AAPTPALPAAKNFSPFASAFGGFLLGSAFMLFAAVVVPSLGVFYNATQAGAVALGGKV
                     ESTIASAEDTLGKSPLYFDAGVAAVGSSFDRSDDFANADAYARGMERNSSFVAGIGER
                     WNDAMTSLALAVFNGGNSILSMILPGANVGSNLAARGTGAAPVRQGMAIVPSTGNAAE
                     DGEVANYIKDSFSDDVKVVRQNDDGTGVIQPVFKSGKQEDYLYVLVPIPDGS"
     gene            18364..19308
                     /locus_tag="VHF05_00510"
     CDS             18364..19308
                     /locus_tag="VHF05_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HVS79442.1"
                     /translation="MTYSHTRKILGFAAAFAFVLTIGAGAVFAAGVQIENIPDMPVSN
                     DFVVGPGKQEMTVAPGESKMIDLTVTNRMADEETFSIETEDIKGSDDLEEPVILLGND
                     RGPYSLKDYVSLPEKEITLQHGEQATIPVTVSVPANAEPGGLYGSVVVSAMPRGSAAG
                     GTAVVSRIATLFFVTVPGGANESGALTSFKVGGDRSVFSGGPIDFQLLFKNEGNVHLN
                     PYGEIDVTNMLGEQVARIIVDPWFALPGSERLREVAWDSGMAFGVYRATARINRGYGD
                     ILDEKTITIVALPGELLAIVIVILAIVVAAAKFAIRRK"
     gene            19315..20091
                     /locus_tag="VHF05_00515"
     CDS             19315..20091
                     /locus_tag="VHF05_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79443.1"
                     /translation="MISRTTHSAIRRISRVIAVSLLSSLFALTAGAQVMQSPSYKMQS
                     DSVNVGGGNSTSTNYGLEDTVGEAGTGEGASASFNLHAGYQQMQEVYLAMTAPGNVVL
                     SPSIGGTTGGQANGATSVTVTTDSPAGYELMIRASGAPALTSGGNSFADYAASAANPD
                     FSWNVPAADSEFGYSPEGSDIAQSFKDDGAACNTGSSDTANSCWAGLTTSDVVIAERL
                     SSNQPLGTATSIKFRAESGTSHVQPAGSYAATTTITAIAL"
     gene            20091..21689
                     /locus_tag="VHF05_00520"
     CDS             20091..21689
                     /locus_tag="VHF05_00520"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HVS79444.1"
                     /translation="MKRILAILVLSALAAMLAPVSGFAANGSFTVGVDVVAVSDTAAP
                     SVPTGVVAAALSSSQIEVDWNVSTDNVAVAGYHIYRNSILIATTTSTTFVDSGLSAST
                     TYAYTVSAFDAAGNESAQSAGASTTTLAATVTPPTPPSGGSSGSSVAMLSIYDVAITA
                     GTDSAIITWNTSFDSAATVSWGKTADAAQGAAGEAMSSRSHAMRIDNLEPGTRYFFRI
                     DALGVNGSRATLGNLSVMTLANANEAPANVKDFTARQEGNGIRLSWQNPSDPDFDSVR
                     IVKSDTFFPNDPQDGEVVYEGRAQDFKDTEVQDGATYYYAAFAKNKKGNYSSGAAASA
                     IYSANGAIIPSNPLDNLPSSTIVDPKIEHLGLQDFQFIQDGQTDAFVDGTKVKIDGSK
                     NLKISIDYAKVPEILKTIMVTLADPSDPEKTFSFILKVNKDKSAYEAVIAPLLKGGEY
                     GVKISIIDYKNRGMKKLEGSLLAAAAEATGGPEPLQGDTLAMLLVIDIVKAFSLVAII
                     IGTLIEAILLTAKGKRQSNHAAHA"
     gene            21673..>23485
                     /locus_tag="VHF05_00525"
     CDS             21673..>23485
                     /locus_tag="VHF05_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79445.1"
                     /translation="MRHTLKQILAQILLLTTTFTVPGVFDLSLIPVPVAEAATFNTQI
                     NYQGKLADSSNVAVADGTYQMSFSLYTAATGGSPIWTESWSGANKVQVTSGLFSVMLG
                     SHQSLSGVNFNQPLYLGVTIESDSEMTPRKALGAVPAAFEAQHAASSTNSDLLDNLSS
                     TQFFRSDAANATSSASTFLSVTQNGAGKIAEFFGPSSNSVFSVLSNGNVGIGSSTPSA
                     KLVVNGSTIVAGTLTVSSLVSTSTATSTFGGGIALSSGCFSIAGICMSNWSTTTSDSW
                     LATKTTDNLTEGSTNLYFTNNRVAAVLAGTTTDALAEGSTNLYFTSARVANYINSSST
                     IANAAGTSVGELLTWNGTRWTAAATSTLGLALSDTIGTLAVGRGGTGINTAPTFGQIL
                     VGNSSSGYTLMATSSLGFLTTNVAEGTNLYFTNARVASVIAGTTTDALAEGSTNLYFT
                     NARADARINATSTIGTITSLPNLALTSGNYTNFGTPFYQFFSATTTSALAEGSNLYFT
                     NARADSRFVTDLAGTSSINSITALNNLATIGTNGATTTVAGGLGVSSSAVVTGSLTAA
                     SIISSGSATSTFAGGIALSSGCFSVNGVCLSSGTNWAT"
BASE COUNT         5284 a         6513 c         6768 g         4920 t
ORIGIN      
        1 gaggaagagg gggagggccc gcttcccgag gcaatgagga ttttcagcct gcatgtatcg
       61 cgaactcgct cttcgatgct gaatccttta ttcgcgcaga ggcgatcgag ggcgattcgg
      121 ccgcgattca ctgatatttc atcgccgagc gttattgtgg ccgcaaaggt cgatattttt
      181 agaccatcgc acatccgctt atggagtaca aaggggaatt agcgttagaa gaagacctct
      241 cattcgaaga gtattatgca gcgatcgacg atatggacga ttccgatctc aatcccggtg
      301 aagaggacga agaggaaggg gaaaacgggg aagatgacga caaggacgag gaggatgagg
      361 aagatgacga cgaatccgaa gagacggacg aagaggagcc gaccgacgag gatctcgaga
      421 ggcttgagga agaaggcagc gacgaatcgg cttaattaaa ttttttgacg ccccgcggct
      481 tgcgccgcgg ggcttttcgt agtgaatttc gcatttccgc gcgcaaaaga ccggttatgc
      541 acctttgaaa cggagtaaaa attgaaacag tcaatttcag cgcggtataa ttgaagaagc
      601 ttctcataca tgccgaagcg cttttgcgcg tgtgcggcag agagctcaac aaaagatgcg
      661 tgttttaatg ttcgggtggg aattcccgcc gtacaatagc ggaggcttgg gggtcgcgtg
      721 cttggggctt gcgcgcgcgc ttcttcgtgc cggactggaa gtctctttcg tgcttccaaa
      781 gcgcctgggc gccgtcaact cggcggtccg cgtcgtattc ggcgacgact attaccgcga
      841 actcggcctc gacacgatca ctctccatga ggttgattca cccctctacc catacctcac
      901 ttcctcggag tatgaagccg ccggcaggag cggcctcaag acgtctccgt acgccaagac
      961 gcttatcggc gaagtcctgc gctacgcgat gactgcggcg aaagtcgcgc gcgacgagga
     1021 gttcgacgtc attcacgcgc acgactggct ttcgtttccc gcaggactcg aggctaagcg
     1081 catttccggc aagcccctga tcgtccacgt gcatgcgact gagttcgacc gcggcggcgg
     1141 caccggcgtg aaccaagaag tctacgacat cgaacggaag ggcatggaag gcgccgacaa
     1201 ggttatcgcg gtgtctcacc ataccaagga tattctcgtc tcgcgttaca gtattccgcc
     1261 ggagaaaata tccgtcgttc acaacggcat cgacgaggcg gactacctcg tgccccacgg
     1321 cacgccgtcg gaaatccagg cgcttcgcgc cgcgggaaac aagatcgtgc tctttgtcgg
     1381 ccgcatcacg atccagaaag ggcccgacta cttcgtaaaa atggcccgga aggttttgga
     1441 atacaagaaa gacgtttact tcgtagtcgc aggttccggc gacatggagt ggaggatggt
     1501 cgaggcggtc gcggccgcgg gcatgtctga tcgcttcatc ttcaccggct tcctccgcgg
     1561 cgaggagttg aacgaagtct atcgcgccgc cgatctctac gtcatgccct ccgtctccga
     1621 gcccttcggc atcacgccgc tcgaagcgaa cttgaatggc acgccggtcc tcatttccaa
     1681 gcagacgggg gcgggggaaa tcctctctca tgcgctccgc tgcgacttct gggataccga
     1741 cgagatggcc aatcaagcgc ttgcggtgct cgagcaccgt tcgctccacg acacgcttcg
     1801 ggaaaacggc cgcgccgaaa cccgcagcat cgactggaac aaggccgcgg agaaatgcat
     1861 cattgtatac gattccgtgg cttaattgac ttttgaataa ttttttacat aattcgatca
     1921 gaatcgctcg ggtctgaaga attcagaaag gagattgaaa tggagaaacc ggcttcccgc
     1981 cgtgaacggg aaaaagatga taagcgcgga aactcacatc gtcagcttga tatcatcgaa
     2041 agaaagtggg gattgcttta tggcttccga tacagtgtca tagccgtagc tgttgtatca
     2101 caaagaatac tttggggacg ttttatatcg ctttatcatg aagcggttaa cctcaagcag
     2161 aattgctttg gttcgctgag tttgcaagaa gcagcggcaa aattcaaaga atgctcattg
     2221 gaaaacctga tttcaattga cctgcgtctg gtaggtgtcg tgcttgtcgc aatcgtattt
     2281 acgatcgcaa tcatgatcga ggaaaatcat ttatacaagc aatctgaagt gtgcgcggat
     2341 gccggtattg agaaggaaaa atcactcaat ataccggatg gaatctatat ccgattgcgg
     2401 caattagaga tgtccaaaca ggctcggttc ttaaggatca ttcatatgag aagcgtcttc
     2461 gcgcttcttc tatttgtcgc tctttgtctg tggatcggtg gcctgatttt ggttgtagat
     2521 caattcatag taccgtggta taatttctaa cggagcatga atatgtttaa gtcccttccc
     2581 gcagaacggc gtccgactga cacgcaatac ttcttcggcc atctgactgg cgcgatactc
     2641 ctgatcattg tttgtgtcgg tgcaatatgg ggtctcagcg tatttttgag actctttcaa
     2701 cactgattaa aaagcaccag cgagagttgg tgctttttca attaaataaa aaatatgttt
     2761 ggaacagcaa agcggcggtg tataattgag aatgtaaagc ctcaaacgcc ttcgatgttc
     2821 tgaaagctgt cgggctataa cctaaaagcc tccctcaaac ttctccaatg tcctccgtct
     2881 gtttttattt ccaagtccat cagccgcatc ggctgaagcg ctataggata ttcgatatcg
     2941 gcaaggaccg gaattatttc gacagtgccg acccccggct ttccaacgag gcgattctcc
     3001 gcaaggtggc ggggaagtgc tatctccccg cgaacaggct tctcctcgac ctcatcagga
     3061 aatatccgaa gttcaaggtg agtttttcga tttccggcac ggcactcgag cagttcgaga
     3121 agttcgcgcc cgacgttttg cagtcgttcg tcgatctcgt cgagacgggc aatgtcgaga
     3181 tcctttccga aacgtactac cactcgctcg cgttcctcta ctcgcccgag gagttcagga
     3241 atcaggtcga gctccacaag aagcttgtga agcgcatctt cggcgtgacg ccgcaggttt
     3301 tccgcaatac cgagctcatc tacaacaacg acattgcccg cgaggttgag cgcatgggct
     3361 acgcgggcat catcgccgaa ggcgccgata ggattctcgg ctggcgcagt ccgaacttcc
     3421 tgtatcggcc tctcattccg gatcgcgctc acaatctcaa gctccttctc aagaactacc
     3481 gcctctccga cgacatcgcg ttccgcttcg gcaacaagga ctgggagggg tatccgctta
     3541 ccgcgccgaa attcggacaa tgggtgtcgg cgcacaacgg caacggggaa gtgctcaatc
     3601 ttttcatgga ctatgagacg ttcggcgagc atcagtggga gtcgaccggc atcttcgatt
     3661 ttcttcgggc gcttccggaa gaagttctga ggaatcctga taatgatttc gtcacgccct
     3721 cagaggcagt gtcgcgctat cgctccgtcg ccgagctcga cattcccgat tatgtttcct
     3781 gggccgacat cgagcgcgac ctttccgcat ggctctcgaa cgacatgcag cgcgaagcct
     3841 tgaaaaacgt ctacgaactc gaggaaaagg tgcgggccgc cgcggacgaa aagctcatgg
     3901 aagactggcg caggcttctc acgtcggacc acttctacta tatgtgcacc aaatggttcg
     3961 cggacggcga cgtgcacaaa tacttcaatc cgtacgaatc gccgtacgac gcgttcattt
     4021 cgtacatgaa cgtcttgaag gatatgcgcg cccgcatcga ggagcgcgaa aggagcgcgg
     4081 aatatagaat tcccatccgc aagctcgcaa ttgcggagta aagtgatgta aaatatctta
     4141 agcaatgctt ttcaagagaa acggcacagc aaaacagggt ccgaaacgct cgctcatgga
     4201 gcgcctcgtt atcgtcgatg cgaacgacgt cgacccggag gaggtcgccg aagcggctgc
     4261 cgaagtcgcg ggaggcgaaa atgaagcgcc ggaaatcggc gtcgtctcct tccgcgcgcc
     4321 ccgaggcgag atccacaacg agctcatctc ctcggaaagt caggaaggcc gtcgccgcaa
     4381 gagcttcaac cctcgcatgt cgttcgatgc cggctccgcg aatctccata cgatctccga
     4441 gcaggagctc gaggatgccg tcgcccaggc gatcgaccgc gaagcccaaa agatcggcgt
     4501 gaagcacttc gaggagcctg cagtttccga agatgatgat ttcgagccgc gcatcacggt
     4561 atccgatccg tcggaattcg ccgccccata tgaagagcct gcggagcttc gcagggagga
     4621 accggaaccg gcagcggaag catatgtgat tcccattgcc gaagaatcgc gtcctattga
     4681 aacggatgaa accgaaacgt acgaggagcg tccgatctcc attcccatcg gcaagacatt
     4741 cgacattctc gaccgcgcca aggagcggcc cgacgaagac gagcggatca tcaagatcgt
     4801 ctaccgcgct ccgaaggtcg tgagcggctt gtatgcacag cccgagtcca agcgccagcc
     4861 gctcgtcttt tccgaagcga tgtctccgat cgccgaagaa gaatccgcgc aggctgaaga
     4921 gagcgtcgaa tcgctcattg acgcggcaat cgcccgcgaa gccgaagatg cgcttgccga
     4981 agcggaagaa ccggcggcag aagcgccggt aggacggccg gataatctac aggatgacgc
     5041 ggaaatcgtc gcacgcgtgc tcgatgagaa cacgtcccct ataacgcacg aggaggcgaa
     5101 agccgccgag aacgacactg ctcccgcttc ctgggtgtcg agcgtcgagg aagcggcaat
     5161 gccgaaaatc tcccatttcg ataaggtgct tttcccggag gaatatttct atgcttcgaa
     5221 cggcgacacc ataaagaccg cccgccagct ctacgatgcg ctgggaaata tggacgatgc
     5281 gaccttcgcg catcacgcga tcgcgcgaaa caccaatgac ttcgcgaact ggatcaaata
     5341 cgtgtacggc catgaggacg tcgcgagaca gcttatgact gcgcgggaga gaactcatat
     5401 gcgccagatt ttagaggatt acttataaaa gtacttgata gtcagtactt ggtaacttga
     5461 agaaacgccc gaaagggcgt ttgattttga taggaaggtg tgatctaatg ggatcgggag
     5521 cgcggaatga aagttcttat attcaatgat gagcagaaaa agctcgatga agtgaaggga
     5581 gtgctggatc atgtactcaa agggtattct gttgaaatca catttgcgca tgttggcaat
     5641 tcgtttgaag cgaatcgggc ttgcggacgc ggcgaattcg atatcatatt cgccgatcat
     5701 tttctcgata agttagaacc atccaataac gccttcagga tcgtattgcg ggcacagcat
     5761 tatggcgcaa gggttttcac caccacggaa gacacggatg tcgctagcaa ggaatacctt
     5821 ccgaaaggaa taccgcatgt cccgctggtg gggcacttga ttgcggaaaa aatactcgag
     5881 cttctcgatg cgaggcagtt caaataatcc tggccggcac agagtgccgg ctctttcttt
     5941 gtccggagag atgcaagctg tagtataatt tgagcatcgg atcggaataa aaattcagga
     6001 ttttatccgc attttttatt tttgatcttt aatttttgat caaatcccgt gtcgcgctct
     6061 ttggttctcg gaaatggaaa tatgctcgtg tgcctggacg aatttgccca ggtgcgcgat
     6121 ttctactatc cgtatgtggg gcaggaggat catctcggcc gcgatctggt ccatcgcgtc
     6181 ggcgtatggg tcgacggaaa gataagctgg ctctccgatg gtacatggaa tatctccgtc
     6241 aaatacgcga atgagacgat ggcgtccgac ataacctgct cgaatgacgg gctgggcgtc
     6301 gagctccatt ttctcgacgt cgtctacaac gaaacgaaca tcttcctgcg gcaggtgagg
     6361 atcaggaata cgcgggccgc ggcgcgccac gcgaaagtat tcttcaacca ggaattccgc
     6421 atttccgaaa acgagcacgc cgataccgcc tattacaatc cgaccgtcga agccctcatc
     6481 cattacaagg gccgccgcgt ctttctcgtg ggcggcacga tcgacggccg gccgttcgac
     6541 gaatatagcg tcggcctctt aggcatcgaa ggaaaagagg gaacgtggaa ggatgccgag
     6601 gatggcgtgc tttcgaaaaa cccgatcgag cacggcgcgg tcgattccgt cgccggcttc
     6661 tcgatggcgc tcgagccgct cgcttcggcg gagttctcat actgggtatg cgcggggcag
     6721 acgtaccgcg aagtccaggc gctccagaaa tacatcatcg cgaaaactcc cgcacacctg
     6781 ctcgaaacga cgcaggattt ctggcatgcc tgggtgaata agataagctt cacgttccag
     6841 gatctggcgc aggaaaccaa aaggctcttc aaggtgtccc ttcttgtcat ccgggcgcat
     6901 tgcgacaacc gcggcggcat tcttgcatcg ggcgactcgg gcaatttcca gcaatacggg
     6961 cgcgatacct acagctacgt atggccgcgc gacggcgcgt tcaccgcgct ctccctcgac
     7021 aaggcgggct acaccgatat cgccgagcga ttcttctcgt tctgcaacga cctcgtcacc
     7081 gacgaaggct atctcatgca caagtaccag cccgacaagt ctctcggcag ctcgtggcac
     7141 ccgtgggtga aggacggcaa ggcgcagctc gccatccagg aggacgagac ggcgacgctc
     7201 atcttcgcgt tctgggagca ttacagggac atgcgcgacc tggaattcgt ggagaagatc
     7261 tataacaatt tcgtgaagct ttgcgccgaa ttcatgcttt cgttccgcga gccgcagacc
     7321 ggactgcccg cggcgagcta cgatctctgg gaagaaaaat ggggcgtgtc tgcctacacc
     7381 gccgcgtcgg tctacggcgc ccttgcggcc gcagcgaatt tcgcgaacct tctcggcaag
     7441 gaggaggaga agctccgcta tgcgcgcgag gcggagcgca tccgcaacct catcatcgag
     7501 tattttctcg acaaggatac gggaggatgg cgcaagctcc tcaacatcgt cgacggcaag
     7561 gccgtgtacg atacgacgat cgatgcttcg agcttttacg gcgcgttccg cttcggcgtc
     7621 cttcccgccg ccgatccgag aatgatcacc tcgcacaagg ttctcaagga caagctctcg
     7681 agcggcgagc ccttttacgg gctcggccgc tacgtcggcg acaagtattt cgccgctccg
     7741 gaaaatgagc tcggcaatcc ttggttcgtc accacgctct ggtcggtcga ataccgcatc
     7801 aatttagccc tgacgcgcga cgatctccag gcgctcgcgg cggaattgaa ccgcatcgcg
     7861 ggcttcgcca cgtcttccgg catcctctcc gagcagatcg acccggcgac ggggaaacag
     7921 ctcagcatct ctcctctcgt atggagccat tcggcgttcg tctcggcagt catcgcctat
     7981 atggaacgcg ccgaaactct cggcctcggc aaggccgtta gcgacgcttc ataatgcaaa
     8041 gccgagaatc acagcagaaa ccgcaggaag aagcgaagcc cgtctcctgg cacgccataa
     8101 gcgtgacgga gtctttttcg atcctttcgg cgggcgatcg cggcctcgcg agcgaagacg
     8161 ccgaacgccg ccttctcgag cacggacgga acgtgttgcc ggaggagccc gtaccgggct
     8221 gggggatgct tttcctgaag caattcatga gcccgttcgt gtacatgctt ctcgccgcga
     8281 gcttcatcgt ttatgccctc ggccagactg acgatgccgt tatcattctc ggcgtcttga
     8341 ttttgaatgc ctgcgtcggc atgatccagg aaggcagggc gcgcgatacg ctcgcggcgc
     8401 tccacgattt caccacgagc gagacgacgg tgctccgcga cggccgggag gagatcgttt
     8461 ccgacgagat cgtcgtgccc ggagatatcc tcgtgctccg cgaaggcgac aaggttccgg
     8521 ccgacgcgcg cgtcatctcc tcccaggggc tttctgcaga cgagtcggcg cttaccggcg
     8581 aatcgacgcc cgtcgggaag actgcggagg cgctcgccga ccagaagctc cccgtcgccg
     8641 agcgggcgaa catggtattc aagggcactc tcgtcgtcgc gggcgaaggc gtggcgctcg
     8701 tcgtcgccac gggcgtcgac acggtgatcg gctccattac gaaaaagatc gtcgagtacg
     8761 acgccgacgt gccgctcaag gaggatatcg ccaaactctc gaagtcgatc acctggagcg
     8821 tgctcgccgc ctgcgccgcg gttttcgcgc tgggcgtcgt tcgcggcgaa tccgtttcgg
     8881 agatgttctt cgtcgctgtc gcggtcgccg tctccctcat tcccgaaggt ctgcccatca
     8941 tcatgacgct cgtcctcgcg ctcggcgtct tcaggatggg gaagagaaac gcgctggtga
     9001 agcggcttca ggcggtggag gccttggggc aggcgacggt catcgccgtc gacaagaccg
     9061 gcacgatcac caagaacgag ctcatggtgt cgcgcctcta cgtcggcggc aaggatttcg
     9121 gggtaaccgg cgacggctat gagcccaagg gcggcatttt cctcgacgat aaggagatcg
     9181 tcgtcggcaa taacggggag ctccttctcg ccgggaaggt cgcggcgctc tgcgccaatg
     9241 ccaccgtcgc gttcgaggag accgaaaagc tctggaaagt gaccggcgat ccgacggaag
     9301 cggcgctccg ggtcttcggc gaaaaatgcg gcttccaaaa agaggggctc gacgccgaag
     9361 cgccgctcgt gttcgaaata cctttcgatt cgaagcataa ataccatgcg acgctccaca
     9421 cgatcggcgg gaagctcctt ctcgcggtgg tcggcgcgcc ggagctcatt ctccaggcgg
     9481 ccgattcgta ttgggagcac ggtgcggcgc ggccgatgac ggagcgcgca cgcgaggatt
     9541 tcaggctccg catcgaggag atgtcgtcca agggcctgcg ggtcgtcggt tttgcgataa
     9601 acgaggacgc gccctcggaa ataagcatcg attccatgcc gccgctcacc tttgccggcc
     9661 ttttcggctt ggaggacgtt ctccgcgaag gcgttcggga agcggtcgag acggcgcagc
     9721 gaaaaggcgt caaggtcgtg ctcatcagcg gcgactatcg gacgacagtg actgcgctcg
     9781 cgcgcgaagc ggggatatgg aagccgggcg acatggtggt ggacggcgcg gacatcgacc
     9841 gcatgagcgc cctctcgcgt tcgatgctca cggtgccgac agtattcgcg cgcgtcacgc
     9901 cggagcacaa gctcatgatt atcgaggcat acaagaagca tggcgatatc atcgccatga
     9961 cgggcgacgg cgtgaacgat gcgctctccc tcgtcgacgc cgatctcggc attgccatgg
    10021 ggcgcatcgg caccgcggtc gccaaggaag ccgcggatat catattgctc gatgacaact
    10081 tctcgtcgat tatcgcggct ctcgaggaag gaaggggaat ctatcgcgcg atcgagcgcg
    10141 tcctcatcta tcttttctcg acgggcctcg gcgagctctt cgtcgtcatc gggtcgctca
    10201 tcttcgcgct tccgcttccg ctcctgccga gccagatcct ctggctcaac gtggtgaccg
    10261 acggcttgcc agtgcttgcg ctcgcgttcg agccgcgcat gcggggagac ggaaagccca
    10321 tcgccaagga tcagaatttc atcgacagcc gctcgctcgt ccggacgacg atcatgggag
    10381 tcgtgatggc cgcgggaacg ctcgcgctct tcgcgctcgg cgcgggagtc gatctcgcgc
    10441 atgcgacgac gatcgcgctc accgccctcg ccgtgtacca gtggtttaat atctacaatt
    10501 gccgatcgaa tgacgcgtcg gtcaccgaaa cgaaaatttt ctcgaacaag agcctcaata
    10561 tcgcaatgat cattaccgtc cttctccact gtgcggcgct ctacgcgccg ttcatgcagc
    10621 gcattctcca caccgtgccg ctcagcttca cggactggat atcggtcgcg ttcgtcgcgc
    10681 tttccgtcat tgccgctgag gaaatcagga agctcgtagt ccggcggaag cgggaacggc
    10741 atgccgcttc cgagccggta gtttctggag tatagtagga gcatgttgaa acttatcgtc
    10801 aaatggcttt ttacggctct ggcggttctc gtggcggccc gattcgttcc cgggatcggc
    10861 gtggcgagcc tcggaacggc ccttcttgtg gcgcttattt taggtctcgt caatgcgatc
    10921 atccggccta ttatcgtcct tttcaccctg ccggtgacta tcctcacgct cgggcttttc
    10981 atacttatta ttaacggagt ccttttctgg ctcgtggcga gcctcgtctc gggcttcgag
    11041 gtcgcaggct tcgtacctgc gttctggggc gcgcttatcg tttcggtcgt gagctttatt
    11101 ggcaatcgtt ttcttcacgc cgtagaacgc aacgacgaat gaaattggcg ctctttttcg
    11161 ttgcttgcaa atttgctcct gcgcagtatt actcatacag cttagtcgca aattcgcttc
    11221 gcgcctcaaa tagcatccaa tttcattcgt cgttaaaagt tagcttagta tgaaaattat
    11281 cactcataac ggttcattcc attcggacga cgtctttgcc gtcgctacgc tttttctcaa
    11341 gttcggaaag gatcacgaac tcatccgcac gcgcgatccc gaaatcatca aatcggcgga
    11401 tatcgccgtc gatgtcgggc acgaatacga tgaatcgcgt ctccgcttcg atcatcatca
    11461 gggtccgccg cttcgccctg acggcatccc atatgcctca ttcggactgg tgtggaaaaa
    11521 atacggagct gaaattgcag ggtcgagcga tgcggcagtg atcatcgagc acgagcttgt
    11581 gcggccgatc gatgccgcag acaacagtat cgatgtcgtc gttccggcaa tcgagggtct
    11641 tttcccgtac ttaattcagg atgtcgtgaa ggcgcttcgg cctacgtggc aggaagatgc
    11701 cgattttgat gccggattca tgcaggcggt cggcatcgcg gataccgtgc ttcgaaggga
    11761 aatcatgcat gctagggcaa agcttgaggc gcaggcaaag cttcaaagtt tttacgacgc
    11821 cgctgcggac aagagaatgc tcatcctcga tcggcattac ccaaaagacg attttctgtt
    11881 cgctcacccg gacgttttgt tcgtgatctt tccgaatgat acgggcgagc cttcatggat
    11941 tgtgcgaacg gtggcggcga aagaaggcgc atttgaaagc cgcaaagatt tgcccgaaag
    12001 ttgggcgggt cttcgggatg cggatttggc caaggttacc ggcgtgccgg atgcggtctt
    12061 ctgccatcgc gcgcgcttcc tggcggttgc gaagacgcgg gaaggggcga tcaagctcgc
    12121 ggagcttgcc ctcaccgctt gattttggtt caatgtattc cgctctaatt ttgacttttg
    12181 aattttaaat tttgaattaa tttcatgttt gtagatgaac tcactataca tatgagggcc
    12241 ggaaaaggag gcgacggcgt cgtctcctgg catcatgagc acagcaagga atacggcggc
    12301 ccggacggag ggaacggcgg caagggcggc gacgtgtacg ccgaggccgt tcgcgatatc
    12361 ggcattctcg cgaaggagcg cttcgagaag gagttcgcgg ccgaggatgg cgaaaacggc
    12421 cgcggctgga gccagcaggg cgcgagcggt gaggatattt acgtgaaatt gcccgtcggt
    12481 tccgtcgtga ccaatctctc gaccgggcag cagatcgaac tgctttccga aggcgaaaaa
    12541 attctcgtac tcaagggcgg acgcggcggg ctggggaacg agcacttcaa ggcgtcgacc
    12601 aatgttactc caaaagaatg gacgccgggc aaagagggag aagaggccga cttccatgtg
    12661 gaattgaggc ttgtcgccga cgtcggtttc gtcggtcttc cgaatgccgg caagtcgagc
    12721 cttttgaacg cactcacgcg cgcccaggcc aaggtcggca gctaccagtt cacgacgctt
    12781 gagccgaact taggcgagat gcacggcttc attctcgccg acattccagg cctcatcgaa
    12841 ggcgcatcgg aagggaaggg tttgggcgac aaattcctcc gccatatcac gcgcacccgc
    12901 acgatccttc actgcatctc cgtcgagaac gaggatgtcg cctccgtgta tgacacgatc
    12961 cgcaccgagc tcaaaaagca tggcgaaggc ctcgacgaaa aagaggagct catcgtcctc
    13021 accaagaccg acgaaacgac ccccgaggat ctcgaaaaga aaatcaagat tctcaagaag
    13081 aagaatcctc gcgtcacgag cgtgacgatt ttggacgacg gaagcgtcac tgcattcaaa
    13141 aacgagctca taaagctcct gcagaagaaa taaaaaacga gccccgctat gcgcggggct
    13201 ctttactact gaatgctata gctgtcgctt gtgccatttt ggacggcatt gatgccatct
    13261 tccaaaaatg gcagattctt ctcttctatg ctcgcgcgca cgtattcttc cgtcacgatg
    13321 cggtaactct cgaattttgc gaagccgaat tctctccctt tttccgtgtc agccaagatg
    13381 cttgcctcgt cttttccgac gaagtagtgc cttttgccct ttgaactccc gatcgagtcg
    13441 ggagcgatgg tgaactcaat aaatgtcatc gcaactctcc tgaattatat aaaatcattt
    13501 ttatttaaaa agtcaatata tatcggtttt cgtagacttt taattatttt cgccctagaa
    13561 tatcgtttat gccatacaag ctcaccgtcg gccttgagat tcatgccgag ctcaagacca
    13621 gcacgaagat gttctgcgac tccaagaacg atccggacga ggagcgcccg aacgtgaaca
    13681 tctgtccggt ctgcatgggg caccccggca cgcttcccgt gatcaacagg gaagcggttc
    13741 ggcacgtgct ccggatgggt acggcggtcg gcggaaagat cgccgatttc accgagtttg
    13801 accgcaagaa ctatttctac cccgacatcc cgaaggacta tcagataagc cagtacaagt
    13861 atccgctggt ccaaggcggc tcgatcgccg gcttcgagct tacccgcatc cacctcgaag
    13921 aggacaccgc atcgtcacac catgaggcag gggactacag cttggtgaac tataaccgcg
    13981 cgggcgtgcc gttgatggag ctcgtaaccg agccggtgat acactcggca gcggatgcgg
    14041 cgcgttttgc caaggagctc cagcttcttt tgcgctatct cggcgcgggc gacgcgaaca
    14101 tggagaaggg cgaaatgcgc gtggaagcaa atatttcggt aagcgcgagc gacgttctcg
    14161 gcacgaaagt cgaagtgaag aacctcaact ccttccgctc ggtggagaag gcgatcgaat
    14221 tcgaatacaa gcggcatgtc gccgcacttg agaagggcga ggagcttgtc caggaaaccc
    14281 gcggctggga cgaagcgaaa caggttacgt tcagccagcg caagaaggaa tcctctcacg
    14341 attaccgcta tttcccggat cccgatctgc cgaagcttaa gatcagcgag attcccgagt
    14401 tctcgcacaa tgcgctccga ggctcgcttc cggagctacc gcaggcgaag cgcgagcggc
    14461 tcgttgcctc gggtctcaag gagtcccagg ccgaagtcct tactgccaat ttcgaactcg
    14521 gcgcgtatta cgacgaagtg atgaacggct ctaccgttct tgcggcggcg gccgagcagg
    14581 tcgcgaacta cgttacttca gacctcatag gcctgttggg cggggagggt gcggctttgc
    14641 ccggcgcgac gaacttccgc aagctcatgg aactcaaggc ggaagggaaa atcccaaccc
    14701 gcgtcaccaa agaccttctg gggttcttag tgaccgaaga taaggatccc gagattctca
    14761 tggaggagaa ggggatcaag gcgggagacg agaacgagct caagcaaata gtcgcgaaga
    14821 tcgtagcgga gaaccagaag gccgtcgatg aatacaaggc cggcaaggaa gcctcgctcc
    14881 agttcctctt gggccagggt atgcgcgcga ccaaaggcac tgccgacccc ggaacgctca
    14941 agaatctcat tctcgaggag atgaaaaagt aagcgaaagc cgcctgcggg gcggcttttt
    15001 tacggttgcc taaacgtcag ggaagcggta aggtactgcc aaacagtact ttgaagggaa
    15061 aggaaaatac gcatgttgta ccgtacaacg ttcagatttg atcctgattt taaggcaatc
    15121 gtgattaccg gcggcccgtg cgccggcaag tcgacattgc tcgccaatgc tcgatgctat
    15181 tttgagaata ggggcctccg ggcgcttgtg ctcacggaaa ccgtcaccga gctttcgctc
    15241 ggcggccttc cgccgcagga gcaatggagg acaccgatat cgttccagcg catggcgctc
    15301 gggtttcagc tcagccgcga gaatgagtac tatcgcatcg cgcaagaact ctccgtgccg
    15361 aaaaagacga ttctgctctg cgacagggga gcgctcgatg gcatggcata tatcaagcgg
    15421 catgaatttc tccgcgtgtg cgaagagctc gagtgcagcc cggctgacct tcttgaccgc
    15481 tatgcgggaa tcatccatct cgtgactgct gcagagggga aggaggaata ttatacgctc
    15541 gccaataatt ccgcgcgcac cgaaagcgcg gaagaagcgc ggcgcatcga caagcggctt
    15601 ctggcggcat gggccggcca cgagaggaaa gctgtcatag gcaattccac cggcttcaat
    15661 gagaaaatgg atcgggggct tcgagcggcg gcgaggttcg cgaacatgcc gggttttgcc
    15721 gattgcgaga ggaaattcat tctcaggaat ttcgcgccct ggttcattcc gcgcagcgcg
    15781 gtgaagattc atatcgagca ggcgtaccta gcattggaag gggaggtgga acggcgcgtt
    15841 cgggcgcgga gaatcgacgg cgccacaagc tactattaca ccgagctcag gccgacgccg
    15901 gagtccggag tcagacagaa agtgaccagt cttattccgc gcagggagta cgaaattctg
    15961 cttgatgaag gaaggggtgt gtatccgacc gtgagcaagg aacgatactg cttcccttac
    16021 gaaggcaagc aattcgaagt cgatcagttt ttgaaaccgc aaggagtacc gatattgttc
    16081 gaagtgaaaa tggagacgat ggatgagccg attcgatttc catcgcactg ggacgcgaat
    16141 gaagtgacag gcaatgcgga attttcaaat cgaaacatcg cgctgcggag ctgaccagca
    16201 gtcgcaaagg cggagatcat tctccgcctt tttctttgta aaaattttcc agtaggggtg
    16261 aaatagggga ggcaagcaag tgtaaccaaa ggccaaaaaa cggaaaaatt tttaatgagg
    16321 agggaataaa aatgcatgaa aaatcgcgcc cggaaacgga cgcgattttg ctgaaaacat
    16381 tggaaaataa tttttgaaaa attccaaaaa gctgagtgaa ggtgaagaga gaatttagtc
    16441 ggaattttcg agatgtttca cgatcagttt atggaccttc tcgcgggagt tgatggaggc
    16501 atctccggtc tcgattccat cgaatacgct ttcaagatcg gaaagccgat agacacgata
    16561 attgttcatc ggatggcggc gggcagggaa ctttccggac ttgtcccagt tgcgaagcgt
    16621 gagaggagag accccgagga tctcagcagc ctgcttgatg gttacgtatg cgtcgctcat
    16681 aaatatatat aaaccttaat aaactttaat tatgattata agaaaactaa taataattat
    16741 caatgtttct aactgttgat aaggtaagaa ttttctatat ttttttatcg ctaaatttaa
    16801 cctactccga attcctaact tggttgcgag taaatctgac cgggtcgcca acttgcggtg
    16861 ttggttctgt ggttgccagc gcagcttcat aatctcgtta ggccggagtg tcaggtgggg
    16921 gaaaggacgg gatagaaaat cctaatcgcg acaggcgagc gggataagtt ttaagttgcc
    16981 gagatcgcca attttgcttc aaggtgcggt tgaaaatttt cagcgaattt gctcatcatt
    17041 ttatccccat atttcatgta cttttcatga ggtgggcggg ctataattta agagtaatga
    17101 atgctgagtt ttttattgat aataaaaaat acatctcggc taaacgagct gccaaactga
    17161 ccggttatgc aaccgactat atcggccaac tggcaagaaa cggcaagata gattcgaaga
    17221 tgatcggacg ctcgtggttt gtgaacgaag aatctcttct cggctacaag aaggatcttc
    17281 tcgacgcgat gttcgcgacc gaaaacgcgc ctttgaaaaa aacgaaaaag cgccgctcgg
    17341 cgcgcaaatc tgcagctccg aaaaataccc cgttcacggc cgaagaatcc gctccaaaac
    17401 tcctgcctgc cggctcgtcc agaatccccg aacttccggg tgtccgcttc atttcgattg
    17461 cggagctcga gattgaactt caaaccaagg cgccgttccg gatggccgcc gttccggaag
    17521 agtctctccc ggtccaagct tccgatacgc ctgtcgaagc gcccgcaccg gttcctgccg
    17581 cacccactcc cgcgcttccg gctgcaaaaa acttttcgcc tttcgcgagc gccttcggcg
    17641 gctttctgct cggctcggcg ttcatgctgt tcgcggcggt cgtcgttccg tcgctcggcg
    17701 tcttctacaa cgcgacgcag gcaggcgcag tcgcgctcgg cggaaaagtc gagtcgacga
    17761 tcgcgtcggc ggaagacact ctcggcaaga gccccctgta cttcgatgcc ggcgtcgcgg
    17821 cagtcggctc gagtttcgac cggagcgatg atttcgcgaa cgctgacgcg tatgcgcggg
    17881 gcatggagcg aaattcctct ttcgttgccg gcatcggaga gcgctggaat gacgcaatga
    17941 catcgctcgc gctcgcggtc ttcaacggcg gcaactcgat cctttccatg attcttccgg
    18001 gagcgaatgt cggatcgaat cttgcggccc gcggaaccgg cgcggcgcct gtccgccaag
    18061 gaatggcgat cgtcccttcg accggcaatg ccgccgaaga cggagaggtg gcgaattaca
    18121 tcaaggattc gttctccgac gacgtgaaag tggtgaggca gaacgatgac ggcaccgggg
    18181 tgatccagcc ggtcttcaag tcgggcaagc aggaagatta tctctacgtg ctggtgccga
    18241 ttcccgacgg gagctgattg cgggcattgc tctgccttac cttgtggggc taaggcagag
    18301 caatgcgcac aagatgcgca gaaaaggtcg aaacattatc aggaatttaa aaacaacaaa
    18361 atcgtgactt actcgcacac aaggaaaatt ctcggcttcg cggcggcgtt cgcgttcgtg
    18421 cttacgatcg gagcaggagc ggtattcgcg gcaggcgtcc agatcgagaa tattcccgac
    18481 atgccggtga gcaatgattt cgttgtcggt ccgggcaagc aggaaatgac ggtcgcgccc
    18541 ggagagtcga agatgatcga tctcaccgtc accaacagga tggcggacga ggagacgttc
    18601 agcatcgaga cggaagacat caagggttcg gacgatctcg aagagccggt gatcctcctc
    18661 ggcaatgatc gcggtccgta cagcctcaag gattacgtct ctcttcccga gaaggagatc
    18721 actcttcagc atggcgagca ggcgacgatt ccggttacgg tttccgtgcc ggcaaacgcc
    18781 gagccgggcg gcctctacgg aagcgtggtg gtaagcgcga tgccgcgcgg cagtgcggcg
    18841 ggaggaacgg cggtagtatc gcgcattgca acccttttct tcgttacggt gccgggaggc
    18901 gcaaatgagt caggcgccct tacgagcttt aaggttggag gagaccgctc ggttttctca
    18961 ggcggcccga ttgatttcca gcttctcttc aagaacgaag gaaacgtcca cttgaatccc
    19021 tacggagaga tcgatgtgac gaacatgctt ggcgagcaag ttgccaggat catcgttgac
    19081 ccgtggtttg cgcttccggg ctcggagcgc ctccgcgagg tggcgtggga cagcggaatg
    19141 gccttcggcg tctatcgcgc gaccgcgcgc atcaatcgcg gctatggcga cattctcgac
    19201 gagaagacca ttacgatcgt cgcattgccc ggggaacttc tcgcgatcgt catcgtgatt
    19261 ctcgcgatcg tcgttgcggc ggcgaagttc gctattcgcc ggaaataatc ccgcatgatc
    19321 tcccgcacaa cgcacagcgc aataagaaga atatcccgcg taatcgcggt ctcgctcctc
    19381 tcgtcgctct tcgcgcttac ggcgggagcg caggtgatgc agagcccgtc gtacaaaatg
    19441 cagagcgaca gcgtgaatgt cggcggcggc aactcgacgt cgacgaacta cgggctcgag
    19501 gacacggtgg gcgaagcggg aaccggagaa ggcgcgagcg cgagcttcaa ccttcacgcc
    19561 ggctaccagc agatgcagga ggtgtacctc gcgatgaccg cgccggggaa cgtcgtcctt
    19621 tctccttcga tcggaggaac gacgggcggc caggcaaatg gcgcgacgtc cgttaccgtg
    19681 acgacggaca gccccgcggg ctacgagctc atgatccggg catccggggc gcccgcgctc
    19741 acgagcggcg gaaacagctt cgccgattac gccgcttcgg cggcgaatcc ggatttcagc
    19801 tggaacgttc cggcggcgga ttcggaattc ggctacagtc cggagggatc ggacatagcg
    19861 cagagcttca aggatgacgg tgcggcatgc aataccggct catcggatac ggcaaacagc
    19921 tgctgggcgg gcctcacgac gtcggatgtc gtcatcgcgg agagattgtc gagcaatcag
    19981 ccgcttggca cggcgacgag catcaagttc cgcgcggagt cggggacgtc gcatgttcag
    20041 ccggccggaa gctacgcggc taccacgacg ataacggcaa tcgcactttg atgaagcgca
    20101 ttctcgccat cctcgtcctc tcggcgcttg cggcaatgct tgctccggtg agcggattcg
    20161 cggcaaacgg gagcttcacg gtaggcgtcg acgttgtggc ggtgtccgat accgcggctc
    20221 cttccgtgcc gacgggggta gtggcggcgg cgctctcgtc atcgcagatc gaagtcgact
    20281 ggaacgtgtc gacggataac gtggcagtcg ccggctatca tatatatagg aatagcattc
    20341 ttatagcgac cacgacttcc acgactttcg tcgattcagg gctctcggca agcacgacgt
    20401 acgcctacac tgtctcggcg ttcgatgcgg cagggaacga atcggcgcag tcggccggcg
    20461 cgtcgacaac gacgctcgcc gcaacggtca cgccgccgac tccgccgtcg ggcggctcct
    20521 cgggaagctc agttgcaatg ctctcgatct acgacgtggc gatcacggcg ggaacggata
    20581 gcgcgatcat cacctggaat acgtcgttcg attccgccgc gacggtttcg tggggaaaga
    20641 cggcggacgc tgcgcaaggt gcggcgggcg aggcgatgag ctcccgctct cacgcgatgc
    20701 ggatcgacaa tctcgagccc ggcacgcgct acttcttccg gatcgacgcg ctgggcgtta
    20761 acggaagcag ggcgactctc ggcaacttga gcgtcatgac gcttgcgaat gcgaacgaag
    20821 ctcccgccaa cgtgaaggat ttcacggcgc ggcaggaggg aaacgggatc aggctttcat
    20881 ggcagaatcc gagcgacccc gatttcgact cggtgcggat cgtgaaatcg gacacgttct
    20941 tcccgaatga tccgcaggac ggcgaagtgg tgtacgaagg cagggcgcag gacttcaagg
    21001 atacggaagt ccaagacggc gcaacgtatt attacgccgc attcgccaag aacaagaaag
    21061 ggaattattc ctcaggcgcg gcggcaagcg ccatctacag cgcgaacggc gcaataatcc
    21121 cgtccaaccc gctcgacaac cttccgtcgt cgacgattgt cgatccgaag atcgaacatc
    21181 tgggccttca ggatttccag tttatccagg acggccagac ggatgcgttc gtggacggca
    21241 ccaaggtcaa aatcgacggg tcgaaaaatc tcaagatctc gatcgactac gcgaaggtcc
    21301 ccgaaatctt gaaaaccata atggtgaccc tcgccgaccc gtccgatcct gagaaaacgt
    21361 tctcgttcat cctcaaggta aacaaggaca agagcgcata cgaggcggtc atcgcgccgc
    21421 ttctcaaggg cggggaatac ggagtgaaaa tctccatcat cgattacaaa aatcgcggca
    21481 tgaagaagct cgagggctcg cttctcgccg ccgcggcgga ggctaccgga gggccggagc
    21541 cgcttcaggg cgatacgctc gcgatgcttc tcgtgatcga tatcgtaaaa gccttctcgc
    21601 ttgtcgcgat catcatcggt acgttgatcg aagcaattct tctcactgca aaagggaaga
    21661 gacagagcaa tcatgcggca cacgcttaaa caaatactgg ctcaaatact tcttcttacg
    21721 acgacattca ccgtgccggg cgtgttcgac ctgtctctca ttccggttcc ggtcgcggaa
    21781 gccgcaactt tcaatacgca aatcaattat cagggcaagc tggccgattc ctcgaacgtc
    21841 gccgtggccg acggcacgta tcagatgtct ttcagcctgt acactgcggc cacgggcgga
    21901 tcgccgatct ggacggaaag ctggtcggga gcgaacaaag tccaggttac gagcgggctc
    21961 ttcagcgtga tgctcgggtc ccatcagtcg ctttcgggcg tcaatttcaa ccagccgctc
    22021 tatctcggcg ttactatcga atcggattcg gaaatgactc cgcgcaaggc gcttggcgcg
    22081 gtgccggcgg cgttcgaggc gcagcatgcg gcgagctcga cgaattccga ccttctcgac
    22141 aatctctcga gcacgcagtt cttccgctcc gatgccgcca atgcgacatc gtcagcctcg
    22201 accttcctct ctgtcacgca gaacggagcg ggaaagatcg cggaattctt cggcccgagc
    22261 tcgaactccg ttttctcggt gctctcgaac ggcaatgtcg ggataggcag ctctacgccg
    22321 tcggcgaagc ttgtcgtgaa tggcagcacg atcgtggccg gcacgctcac ggtttcctcg
    22381 cttgtctcga cctcgaccgc aacgtcgaca ttcggcggag gcatcgcgct ttcgtccgga
    22441 tgcttctcga tcgcgggtat ttgcatgagc aactggtcga ccacgacttc cgacagctgg
    22501 ctcgcgacaa agacgaccga caatctcact gaaggatcga cgaatctcta cttcactaat
    22561 aatagagtgg cggccgtatt ggcaggcacc acgaccgacg cgctcgcgga gggaagcact
    22621 aatctctact ttacttctgc gcgcgtcgcc aactacatca attcatcgag cacgatcgca
    22681 aacgcggccg gaacttccgt aggagagctt cttacttgga atggaacgcg ctggaccgcc
    22741 gccgcgacgt cgaccttggg actggctctc tccgatacga taggcacgct tgcagtgggg
    22801 aggggaggca cgggcatcaa tacggcgccg acattcggcc agattctcgt cggcaattcc
    22861 tcgagcggct acaccctcat ggcgacgtcg tccttgggct ttctcacgac gaacgtcgcc
    22921 gaaggcacga atctctactt cacgaacgcc cgcgtcgcct ccgtaatcgc gggcactacc
    22981 actgacgcat tggctgaggg ttcgacaaat ctctacttca caaatgcgag ggcggatgca
    23041 agaatcaacg ccacatcgac catcggtaca atcacgagcc tgccgaacct cgcccttacg
    23101 agcggcaact acaccaactt cggaacgccg ttctatcaat tcttctccgc aaccacgacc
    23161 tcagctctcg ccgaaggctc gaacctctac ttcaccaatg cgcgggcaga cagccgtttc
    23221 gtaaccgact tggcgggaac gtcgtcgatc aattcgataa ctgcgctgaa caatctcgcg
    23281 acgataggaa cgaacggcgc aaccacgact gttgcaggcg gcctcggcgt atcatcctcc
    23341 gccgtcgtca ccggctccct caccgccgcc tcgatcatct cgagtggttc ggcaacttcg
    23401 accttcgccg gcggcatcgc cctctcctcc ggctgcttct cggtgaacgg cgtttgcctc
    23461 tcctcgggca ccaactgggc gacta
//
LOCUS       DASZYS010000008         5750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_972145, whole genome shotgun sequence.
ACCESSION   DASZYS010000008 DASZYS010000000
VERSION     DASZYS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972145"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHF05_00530"
     CDS             <1..723
                     /locus_tag="VHF05_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVS79446.1"
                     /translation="EAPVLEFKNVGFKYEEGKAVLHDASFALDRGKTYALVGPTGGGK
                     TTTASLMARLYDPTSGAVLLDGMDIRSFEPAERTKRIGFILQEPYMFTGTVRDNILYG
                     SDEYAKLSNEELEAELEKAGLKGLLARFEQGLDTPVASTGGALSLGQRQLVAFIRAVL
                     RKPEILILDEATANIDTVTEKLLGEILEKLPASTTRVVIAHRLNTIENADEIFFVNGG
                     EIVPAGSLEHAVGMLMHGKRES"
     gene            896..1678
                     /locus_tag="VHF05_00535"
     CDS             896..1678
                     /locus_tag="VHF05_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVS79447.1"
                     /translation="MHTERTKAAVLEVSDLQVRYGDFTAVDGVSFSVNPGEIFGLLGP
                     NGAGKTSTLSAIEGLLSPSNGSVSVAGFDIFKEPLEARANIGIQLQFTSFQQDLSVRE
                     IVRLYASLYGTPLSDKEIDDLLQEIKLEGQGDKRARELSGGQQQRLSLSIATIHNPPL
                     VLLDEPTSGLDPQSRRQLWERVEKMRDSGRSVLLTTHSMEEAKAICERVAIVDHGVIV
                     ETGNPQELIDKYRNDPDVLAVSRGEVTLEDVFIGLTGKEVRV"
     gene            1686..2453
                     /locus_tag="VHF05_00540"
     CDS             1686..2453
                     /locus_tag="VHF05_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVS79448.1"
                     /translation="METTEKNITRPIPNAKEALRALLRADWSIQWRNRRAMLMSIFVP
                     VIFIISWKSLVPVVGGAAVLSICIAIGLPAIGLMGYSGSVARDRERGVFQRLRAAPIP
                     TWSIMTSRILVQIGVMAFMTLCTILVGYFNDGIHINLPEGLLIVIFSAIAGLSFLGLG
                     QLVVGLIRSSEAVNAAARLVYFPVAVVGAIAEIGVFGDILKMIVDWSPLGTTKTLIAA
                     AATLSFDSSVWLALFLTLAYGVVFAAIGIKKFDWSVN"
     gene            complement(2595..3323)
                     /locus_tag="VHF05_00545"
     CDS             complement(2595..3323)
                     /locus_tag="VHF05_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVS79449.1"
                     /translation="MKISIVIPAYNEESSIADTIRAALAQDYPDFEVIIVNNASSDKT
                     AEVAARFPVRVVTEEKKGILFARERGRLEAKGELVANLDADCLPAPHWLSTGAKYFTD
                     PEVAAVTGPYDYYDGTRVFRKVSFASQRGIYRLVNNLMDLLDRGGILIGGNNMIRASV
                     LEAAGGYNTKIKFYGEDTDTARRVSEHGRIVFSKDFVMRTSARRFKTEGTVKITSYYL
                     YHFLKAGVLPAIGFDKGTQVENEL"
     gene            3536..4099
                     /locus_tag="VHF05_00550"
     CDS             3536..4099
                     /locus_tag="VHF05_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVS79450.1"
                     /translation="MIETEHELSDEDLVSLIRQGDKEKFGILMARYEEKLFRYARRFL
                     SKNEHIEDMVQEVFVKAYTNLQSFDLSQKFSSWIYRIAHNTFVNELRRQSRTPISFFD
                     FDTLVGYSGSDSTAEEEFEREELKKMIDKSLSLLKPQAREAIILHYLEDLSYREIADI
                     LHVPIGTVGVRLKRAKDALRREYEKLG"
     gene            4112..4903
                     /locus_tag="VHF05_00555"
     CDS             4112..4903
                     /locus_tag="VHF05_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79451.1"
                     /translation="METEHTKEKNSSLSEKILEKIEKGEVKMRPKAYFAAKIAALIAV
                     AALVFLTTLFLASFIIFDLRASHRLDLLGFGFAGIVAFFAAFPWFVLLVDIALFVLLE
                     WLLRKFTIGYHKPVLYIVGTTAVVLVGLGVAVGLSPLHPFLLVKERAAPGPVMGGLYG
                     EHHMSKPEDGIFAGTVTSTATSTFTMVGLVDDDPWSKKATTTVEAPQGFRPQEFLKPG
                     DRVLVIGKQDDGRIEAYGVKRIAVTLMQIVSPPDGQPVKFRIEFE"
     gene            4986..5507
                     /locus_tag="VHF05_00560"
     CDS             4986..5507
                     /locus_tag="VHF05_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HVS79452.1"
                     /translation="MKKFFLVAGLAIAFAGYAIFQKYGSTPVGGALADAGGGSGANDI
                     AQPSLPGSVPDQSAPSSNNAPSSTPIPAPTEDSATGYKDGTYTGVVADAFYGPLQVSA
                     TITNGKIADIKFLKYPNDRPESIEVNTDSNPILEAEAIRSQSANVDVVSGATQSSEAF
                     SQSLAAALAQARQ"
BASE COUNT         1281 a         1577 c         1634 g         1258 t
ORIGIN      
        1 gaagctccgg ttctggagtt caagaacgtc ggattcaaat acgaggaagg caaagccgtg
       61 ctccatgacg cgtcgttcgc gctcgatcgc ggcaagacct acgcacttgt cggtccgaca
      121 ggcggcggaa aaacgacaac ggcatcgctt atggcgcggc tctatgatcc cacttccgga
      181 gccgtgcttc tcgacggtat ggacatcagg agcttcgagc cggcggagcg gacgaagcgc
      241 atcggattca ttctccagga gccgtacatg ttcaccggaa cggtgcgcga caatattctt
      301 tacggaagcg atgagtacgc gaagctctcg aacgaagagc tcgaagccga actcgagaag
      361 gcgggattga agggccttct cgctcgcttc gagcagggcc tcgatacgcc ggtcgcttcg
      421 acgggcggcg cgctctcgct cggccagcgc cagctcgtgg cgttcatccg cgcggtcttg
      481 cgcaagccgg agatccttat cctggacgag gcgaccgcga acatcgatac ggtgacggaa
      541 aagctcctcg gggaaattct cgagaagctc cccgcatcga ccacgcgcgt cgtcatcgca
      601 caccggctca ataccatcga gaatgccgac gaaatattct tcgtgaatgg cggggaaatc
      661 gtgcccgcag gctcgctcga gcacgcagtc ggcatgctca tgcacggcaa gcgggaaagc
      721 taaaggaagc caggtgggaa gaaaataaaa tttctaattg acctaattaa tctaatttct
      781 aaacaaatcc caatatccaa gtctcaatga ctaattggat ttgatgcttg attattattt
      841 atgtaattag aataattagg aattaggtta atttaaattt gatataatca aagcaatgca
      901 tacggaacgt acgaaagcgg cagtcctcga agtgagcgat ttgcaagtcc gctacggcga
      961 tttcacggcc gtcgacggcg tgagcttcag cgtaaatccc ggggaaatct tcggactcct
     1021 gggccccaac ggcgcaggca agacctccac gctctcggcc atcgaagggc ttctttcgcc
     1081 gtcgaatggc tctgtttccg tcgccggatt cgacattttc aaggagcctc tcgaggctcg
     1141 cgccaatatc ggcatccagc tccagtttac gagcttccag caggatctct cggtgcgcga
     1201 aatcgtgcgg ctttacgcga gcctctacgg cacgccgctt tccgacaagg agatcgacga
     1261 ccttctccag gaaatcaagc tcgaggggca gggcgacaag cgcgcccgcg aactttccgg
     1321 cggccagcag cagcgcctct cgctttccat cgcgaccatc cacaacccac cgctcgtcct
     1381 tctcgacgag ccgacttcgg ggctcgatcc gcagtcgcgc cgccagctct gggagcgcgt
     1441 tgagaaaatg cgcgacagcg gacggagcgt gcttctgacg acccactcga tggaggaggc
     1501 caaggcgatc tgcgagcgcg tggcgatcgt cgaccatggc gtgatcgtcg aaacggggaa
     1561 tccgcaagaa cttatcgaca aatacaggaa cgatccggac gttctcgcgg tgtcgcgcgg
     1621 ggaagtgacg ctcgaagacg tctttatcgg ccttacgggc aaggaagtcc gcgtttaaaa
     1681 tatatatgga aacaaccgag aaaaatatca cgcgtccgat accgaatgcc aaagaagcgc
     1741 tcagggcgct ccttcgcgcc gattggagca tccaatggcg gaaccgccgt gcgatgctca
     1801 tgagcatctt cgtgccggtt attttcataa tttcctggaa gagcttggtt ccggtcgtgg
     1861 gcggcgcggc ggtgctttcc atctgcatcg ccatcggcct tccggcgatc ggcctcatgg
     1921 gctactcggg ctccgtcgcc cgcgaccgcg aacgcggcgt cttccagcgc ttgcgcgccg
     1981 ctccgatccc gacatggagc atcatgacgt cgcgcatcct cgtgcagatc ggcgtcatgg
     2041 ccttcatgac tctctgcacg atccttgtcg gctatttcaa tgacggcata catataaatc
     2101 ttccggaggg attgcttatc gtcatcttct ccgcaatcgc agggctttcg ttcctgggac
     2161 tcggccagct cgttgtcggc cttattaggt cgagcgaagc ggtgaacgcc gcggcgcgcc
     2221 tcgtatattt tcctgtggca gtcgtcggcg ccattgccga gatcggcgtg ttcggcgaca
     2281 tcttgaagat gatcgtcgat tggtcgccgt tgggcaccac caagaccctc attgcagcgg
     2341 ccgcaacgct ttcattcgat tccagcgtat ggctcgcgct ttttcttacg cttgcgtatg
     2401 gcgtcgtttt cgccgcgatc ggcatcaaaa aattcgactg gtcagtgaac taagaagggg
     2461 aaagtttaga gttgcgagtt gctagttttt agaaagaagg aaaacaagca gtaaaaaaat
     2521 gaagcccggg gtccgctcat aagatcgaac ctcgggcttg cgcgccggaa tcggcgctgt
     2581 gtgcggccgc gcctttatag ctcgttttcc acctgagtgc ccttatcaaa gccaattgcg
     2641 ggaagaacgc cggctttgag gaaatggtag agataatagg aagtgatttt gacggtgcct
     2701 tcggtcttga agcggcgcgc ggaggtgcgc atgacgaaat ccttcgagaa gacgatccgg
     2761 ccatgctcgg aaacgcgccg cgcggtgtcg gtgtcctcgc cgtagaactt gatcttggta
     2821 ttgtagccgc cggcggcttc gaggactgac gcgcggatca tgttgttgcc gccgataagg
     2881 atcccgcccc tgtcgaggag atccatcagg ttgttcacga ggcggtagat gccgcgctgg
     2941 ctcgcgaacg acactttccg gaacacgcgc gtcccgtcgt aataatcgta gggccccgtt
     3001 accgcagcga cttcgggatc agtaaaatat ttcgcgcccg tggaaagcca gtgaggagcg
     3061 ggaaggcagt cggcgtcgag attggcgaca agctcgccct tcgcttcgag gcggccgcgc
     3121 tctctcgcga agaggatgcc tttcttttcc tccgtcacga cgcggacagg gaagcgcgcg
     3181 gcaacctccg ccgtcttatc gctcgacgcg ttgtttacga tgatcacctc gaaatccgga
     3241 taatcctgcg ccaaagcggc gcggatggtg tccgcgatcg aggattcttc attgtatgca
     3301 gggatgacga ttgagatttt catgcggggc taaatcggaa ccgaacagat tatattatat
     3361 gcctcttgcg tgcgaagcca gggctttatc accaggtgtg cgccagtaag gtgcgaaaaa
     3421 ccctttattc tgcgtctttt tgaaagaatt caggcttcag ggcgtaatat atggaagccc
     3481 gggagatttg gctgatatac taaaggttca agccgcaatg gcgcggcacg agcgtatgat
     3541 tgagacagag catgaattga gcgacgagga ccttgtaagc cttatccgcc agggcgacaa
     3601 ggagaagttc ggcatcctca tggcgcggta tgaggagaag ctttttcgat atgcgcggcg
     3661 gtttctctcc aagaacgagc acatcgagga catggtccag gaggtttttg tcaaggcgta
     3721 tacgaatctc caaagtttcg acctttcgca gaaattctcc tcctggatct atcggatcgc
     3781 ccacaacaca ttcgtgaacg agctgcgccg gcaatcgcgg acgccgatct cgtttttcga
     3841 tttcgacacg cttgtcggat attccggaag cgacagcact gcagaagagg agttcgagcg
     3901 cgaggagctg aagaaaatga tcgataagtc gctctcgctc ctcaagccgc aggcccgcga
     3961 agcgatcatc ctgcattacc tcgaagacct gagctaccgc gagatcgccg acattctcca
     4021 cgtcccgatc ggcacggtgg gcgtgaggct caaacgcgcg aaagatgctt tgaggaggga
     4081 atatgaaaag ctcggctaag ctcgaaacac tatggaaact gaacatacaa aagaaaagaa
     4141 cagcagtctc agcgaaaaaa ttctcgagaa aatcgaaaag ggcgaagtga agatgcggcc
     4201 gaaggcgtat ttcgcggcga aaatcgcggc gctcattgct gtcgcggcgc tcgtatttct
     4261 cacgacgctt tttcttgcga gcttcattat cttcgatctt cgcgcgagcc ataggctcga
     4321 ccttctcgga ttcggatttg caggcattgt tgcgttcttc gccgcatttc cctggttcgt
     4381 gcttctcgtc gacatcgccc ttttcgtcct tcttgagtgg cttctcagga aatttaccat
     4441 cggataccat aagccggtgc tctacatcgt aggcacgact gcggtggtgc ttgtggggct
     4501 gggagttgct gtcggccttt cgccgcttca tccttttctt cttgtgaagg agcgcgccgc
     4561 gccgggaccg gtcatgggcg gcctgtacgg cgagcatcat atgtccaagc cggaagacgg
     4621 catttttgcc ggtaccgtca catcgaccgc tacctcgacg ttcaccatgg tagggcttgt
     4681 cgatgacgac ccctggtcca agaaggcgac cacaacggtc gaagcgccgc agggttttcg
     4741 cccgcaggaa ttcctgaagc cgggcgacag ggttcttgtc atcggaaagc aggatgacgg
     4801 caggatagag gcatacggcg tgaagaggat tgccgttacc ctcatgcaga tcgtctcgcc
     4861 gccggacggg cagccggtga aattccgcat tgaatttgag tagcggctga tataatttcc
     4921 tagtcgcggt gtagtagaat aggtcaattc aggagaaatc ctggttttcg ccgaatcaac
     4981 aatccatgaa aaaatttttc ttagtggcag gccttgcgat cgcgttcgcc ggatatgcga
     5041 ttttccaaaa gtacgggagc acgcccgtag gaggcgctct tgctgacgcc ggcggaggtt
     5101 cgggagcgaa tgatatcgcc cagccatcgc ttccgggaag cgtgcccgat cagtccgcgc
     5161 cttcttcaaa taacgcgcct tcatcaacgc cgattccggc cccgactgag gattcagcga
     5221 ccggatacaa ggacggcacg tataccggcg ttgtcgccga cgcgttctac ggcccgcttc
     5281 aggtctctgc aacgattaca aacggcaaga tcgccgatat taagttcctc aaatatccga
     5341 acgacaggcc ggaatcgatc gaggtgaaca ccgattcgaa tccgattctc gaagctgagg
     5401 ccatcaggtc gcagtcggcg aatgtcgatg tcgtctcggg cgcgacgcag tcgagcgagg
     5461 cttttagcca gtcgcttgct gccgccctag cccaggcccg gcaatagctt gcaaaacgca
     5521 cacgctgctt cgttggaggc aaaattttct cccgcgcagt actacttgta cagcttggtc
     5581 gcaaatttcg cctccgcctt gcatcgcatc gcgttttcca acctataaaa tttcaaaaaa
     5641 cattgaaaga aacaagaatt ataatgggaa tgcccgttac ggtcgagatc gccgatcgtt
     5701 ccgccacaca aaatgatgtg gaggaggtgt acagttattt cgattacatc
//
LOCUS       DASZYS010000009         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_589552, whole genome shotgun sequence.
ACCESSION   DASZYS010000009 DASZYS010000000
VERSION     DASZYS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589552"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1357)
                     /gene="ftsA"
                     /locus_tag="VHF05_00565"
     CDS             complement(179..1357)
                     /gene="ftsA"
                     /locus_tag="VHF05_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HVS79453.1"
                     /translation="MSRNIAVGIDIGTYQVKVVVASNSDENERGLPVVLATGIAESKG
                     LRHGYIQNQGEVSRSVLQAVRQAEKTAGFKIKDAFLSIGGVGLTGIIAQGSTVVARGD
                     SEIAELDISKVQEACESEIPASQMSNRKIINAVPIQYKIDGKVVLGKPLGMKGMRLEA
                     KMLFVTCLEPHLQDLIQAVEEAGIEVVDVVASPIAGAVVTLTKTQKIAGCVLANIGAE
                     TVSIVVYENNIPISLEVFPLGSTDITNDIALGLKIPLEEAEQIKLGAVTSTPVSKRRF
                     DEIVNARLSDIFELIEAHLKKIGRSGLLPAGVIITGGGSNVGAIDFLAKSALRLPAKI
                     GAITLSDKKGVIRDSTWSVAYGLCIIGLLQEGSGTNLSGVFSRGAGTKIWGWFKQFLP
                     "
     gene            complement(1480..1872)
                     /gene="ybeY"
                     /locus_tag="VHF05_00570"
     CDS             complement(1480..1872)
                     /gene="ybeY"
                     /locus_tag="VHF05_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVS79454.1"
                     /translation="MSKESENFAIRQTTKERVPKLPFEAMKNAALGKKYSLSIVFCGE
                     KRMRTLNHTHRRIDKPTDILSFPLDEESGEIFISIKNSRKMMRDFGRELDNFIGFLFI
                     HGLIHLKGFDHGSRMEAEEVKLRRRFKI"
     gene            complement(1872..2225)
                     /locus_tag="VHF05_00575"
     CDS             complement(1872..2225)
                     /locus_tag="VHF05_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HVS79455.1"
                     /translation="MESQTQSSVPHAMVTTGFELDNYRVVKNLGVARGIVVRSRSIVG
                     NIGAGLQTLFGGNITLYTELCEKTREDAFDLMLEHAGEMGANAVIGARYDATEIMNGV
                     TEVLAYGTAVVVEPK"
BASE COUNT          559 a          651 c          728 g          587 t
ORIGIN      
        1 cctgcgactc accgtgcatc ttatcgagaa ggatatctct cgcaagcgga tcaggtcgtt
       61 tcgaaaactg cttcggcagg acagccattg gctggttgtc gaagaataaa aacaagtccg
      121 ggcattactg cctggacttt cttttttaaa tcataaattc caacaggcgg atttttggct
      181 acggcagaaa ctgcttgaac cagccccaga tcttcgtgcc ggctcctctg gaaaagacgc
      241 cgcttaggtt cgtgccgctg ccttcctgaa ggaggccgat gatgcaaagc ccgtaggcga
      301 ccgaccaggt cgaatcgcgg atgacgccct tcttgtcgga gagggtgatc gcgccgatct
      361 tcgcgggcag gcgcagggcc gacttggcga gaaagtcgat cgcgccgacg ttcgagccgc
      421 cgccggtgat gatgacgcct gcgggaagga ggcccgagcg gccgattttc ttcagatgcg
      481 cttcgatgag ctcgaagatg tcggagagcc gcgcgttcac gatctcgtcg aaccgccgct
      541 tggacacggg agtcgaagtg acggcgccga gcttgatctg ctccgcttcc tcgagcggga
      601 tcttgaggcc aagggcgatg tcgttggtga tgtcggtgga gccgagcgga aagacctcga
      661 gcgagatggg aatgttgttt tcgtacacga cgatggagac ggtttccgcg ccgatattcg
      721 caaggacgca gccggcgatc ttttgggtct tggtgagggt gacgaccgcg ccggcgatcg
      781 gcgacgccac gacgtcgacc acctcgattc cggcttcctc gactgcctgg atgagatcct
      841 ggaggtgcgg ctcgagacaa gtgacgaaga gcatcttggc ttcgaggcgc attcctttca
      901 tgccgagcgg cttgcccagg acgactttgc cgtcgatctt gtactggatg gggacggcgt
      961 tgatgatctt gcggttcgac atctgcgagg ccggaatctc cgactcgcag gcttcctgaa
     1021 ccttgctgat gtcgagttcg gcgatttccg aatcgccgcg ggcgacgacg gtcgagccct
     1081 gggcgataat gccggtgagg ccgacgccgc cgatggagag gaaggcgtct tttatcttga
     1141 agccggcggt tttttccgcc tggcggacgg cctgaaggac tgagcgggag acctcgccct
     1201 gattttgtat gtagccgtgg cgcaaacctt tcgattcggc gatgcccgtg gcaaggacga
     1261 cgggcaggcc gcgctcgttc tcatccgagt tcgacgcgac cacgactttc acctggtagg
     1321 tgccgatatc gattccgaca gcgatgttgc gtgacacggt aataaggtca aattaaaaca
     1381 tcaaaatgca aaatgcaaaa tgacaatgca aaaatcaaaa attgaagaaa gaaaatcgaa
     1441 tcagaataca aagcgtttcg cctttgcctg agcaaagaat taaattttga agcgccgtcg
     1501 aagcttgact tcttcagctt ccattctact accatgatcg aatcccttca aatgaataag
     1561 gccgtggata aaaaggaatc cgatgaagtt gtcgagttcg cggccgaagt cgcgcatcat
     1621 tttccgcgag tttttgatcg aaatgaatat ctcgccgctt tcctcgtcga ggggaaagga
     1681 gaggatgtcg gtcggcttgt cgatccggcg gtgcgtatgg ttcaaggtgc gcatgcgctt
     1741 ctcgccgcag aaaacgatcg aaagggaata tttcttgccg agcgcggcat tcttcatcgc
     1801 ctcgaagggg agcttcggaa cgcgctcctt cgtggtctgc cgtatggcga aattttccga
     1861 ttctttgctc attatttcgg ctctacgacg accgccgtgc cgtaggcgag cacttctgtc
     1921 acgccgttca tgatttcagt tgcatcatac ctggctccga taaccgcgtt cgcgcccatc
     1981 tctccggcat gctcgagcat gaggtcgaac gcgtcctcgc gcgtcttctc gcagagttcg
     2041 gtgtagaggg tgatattgcc gccgaagagc gtctggaggc cggcgccgat attgccgaca
     2101 atcgagcgcg agcggacgac gatgccgcgg gcgacgccaa ggttcttcac tacgcgataa
     2161 ttatcaagct cgaagccggt cgtgaccatc gcatggggaa cggatgattg tgtttgtgac
     2221 tccataataa tgaattattt gttatttcta gcgtacgctt ttgaccgcca aaaacaagcc
     2281 ttaattgata ttctataatt cctgcctacc gcaggcaggc tgcagaatta tagaatatca
     2341 attaaaagca aaagccgcct tgcggcggct tttgtctgct tcaaaaataa tacaaagccg
     2401 gtcttccggc ttttccttca gaacgttgga gtgaagaatt ttgattttaa aacattgcgc
     2461 aggaatattt ttagcaaaaa atattccgag catagcaaaa gccgcccgac tggggcggct
     2521 ttatg
//
LOCUS       DASZYS010000010        11601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1234693, whole genome shotgun sequence.
ACCESSION   DASZYS010000010 DASZYS010000000
VERSION     DASZYS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234693"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..745)
                     /gene="frr"
                     /locus_tag="VHF05_00580"
     CDS             complement(194..745)
                     /gene="frr"
                     /locus_tag="VHF05_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVS79456.1"
                     /translation="MAYNFAPFQQKVKESEDWLKQEYQGLRTGRATPAILDLVKVESY
                     GTRMPLNQIGGISIEDARTLRITPWDMSQVKSVEKALMESDLGLGVSVDEKGLRVKFP
                     ELTAERRDQLVKVARQKLEDARVALRSEREKTWDDIQKKEKEGGMSEDEKFRLKDQMQ
                     KIVDEANKRLEEMAEKKEQEIAA"
     gene            917..1561
                     /locus_tag="VHF05_00585"
     CDS             917..1561
                     /locus_tag="VHF05_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79457.1"
                     /translation="METQERLSCFLCKRYLGNVTFTLDEIIHSKPIRQCRLFTIQPGK
                     HNEVPSTLEQVIGRSAHHYVTFSERKSIGSLHEGRGRTFQNENDSPTLHVFGDRVGQT
                     ILVCNSCCEHAGNELINLLDAEYGSRLGQRVNRICRILLEKNIVRFDAETLKTFVKEF
                     GSSVLSDSWTATLKELNEIAVYDDVASLLDCLRPLIDEFIASGHDNSARLKLVS"
     gene            complement(1685..2470)
                     /locus_tag="VHF05_00590"
     CDS             complement(1685..2470)
                     /locus_tag="VHF05_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79458.1"
                     /translation="MENNNLDLLRLKVWLVGANILFEAREYMNTLARTVDGVDEMIDL
                     IPFGMVKERILIMFVILFDRRRTLEFDQICRLCEEGHDLISRFSLNDSGFNIMLREFH
                     SLISDSFVELDRKRNQAAIVELALKAMRDAPAVCFAEASDWILKAKKEIQTEVTIKNI
                     VSAFERAHRPIPEELTAYFAELIRTKFGLSGLDTIACDIQSLRSQAVEMFARRCKTDP
                     IGMLMSCHTEHPRCTALLAAGLSLRVHEIPISPELGAAAGASV"
     gene            complement(2537..3217)
                     /locus_tag="VHF05_00595"
     CDS             complement(2537..3217)
                     /locus_tag="VHF05_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79459.1"
                     /translation="MDIIRDAHKNKNLHHAYLIEGPLAENFAALVEFLEEAGFSTRGN
                     PDFWTGEFETMGIDEGRVLSDKELRRAIGGGWKVFVVRAEGITVEAQNSLLKVLEEPT
                     EGTHFFFLMPSGERLLPTLRSRLEIVPARAEAAAEARKDAKKFLMMNQAERGEFLKPV
                     VTEKDKSGALLFVSALEVEVRANLSLASARREDIFVLEEIEKGRQYLLDRSPSVKIIL
                     EHIAAIAP"
     gene            complement(3581..4594)
                     /locus_tag="VHF05_00600"
     CDS             complement(3581..4594)
                     /locus_tag="VHF05_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019553311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVS79460.1"
                     /translation="MGILSRKIGIDLGTANILVFVPGRGIVLNEPSVVAVSEEDNRIL
                     AIGLEAKEMIGRTPDNIIAYRPMKDGVIADYRVTEAMLRYYIERALGRWNFVKPDVLV
                     SVPAGVTSTERRAVIEAAIKAGAKNAYVVKEPILAAIGAGIPIQEAVGHMIVDIGGGT
                     TDVAVISLGGIVAAASVKVAGNRLDQAIADYIKKTFNLGIGDKTAEDIKIKVGSAIPL
                     DEELSMTIKGRDFISGLPRSVEIKTNEIVKAIGKELRDMVKAVKDVLQETPPELAADI
                     IDRGIIMTGGTSQLRNLPELMYRRTGVKATLADEALFCVVKGTGVSLDHLDTYKRTII
                     SKR"
     gene            4927..5715
                     /locus_tag="VHF05_00605"
     CDS             4927..5715
                     /locus_tag="VHF05_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HVS79461.1"
                     /translation="MKKNKKIWLVVGLLVIVIVFVILNIPSIKKPFGQTETGKISLEV
                     PLEGSEVYLDGKGYQSTSVKNQTLDIPDVAANTPHTVLVAKNGYWPWGKTVTVEAGRT
                     LDLTAFNGPQAEVTTDVAAGSKDYAAATAALATNTGTPSKATMIVSKDGSVGIWVEGN
                     ALMAEWKGEDDPDQFFCVEGLCDKDTQVFKGTAPITHLAFYKNRDDIVLFSSGGAIYA
                     IELNRNGTQNFQPVFMGTNPTFAPSSDGTVLFVKDGGKIQEVLL"
     gene            complement(5924..6547)
                     /locus_tag="VHF05_00610"
     CDS             complement(5924..6547)
                     /locus_tag="VHF05_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79462.1"
                     /translation="MAFETGYLLPTREEFARRDRELAEALQKEKERQDQFHIEVYKEA
                     GANRRSLHTNRWAILGGVVSTQLILAGAYNYMFNNITVRSSPPQTITAFFGNVFKVNW
                     KAYVELFDNTHVLSTAQPALFFIPLLALVIIIGAWLLERRLRDLYIECTVEGSNIEKK
                     FGILTGVYSRLLKPRRAILDIIDHTHAFNGVVIGAVLMWSFLFYHAF"
     gene            6659..8545
                     /locus_tag="VHF05_00615"
     CDS             6659..8545
                     /locus_tag="VHF05_00615"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79463.1"
                     /translation="MKILSVYPISRGISKEKLSYFSSEDHALGSIISVPLRKRSVPAI
                     VVGSADAETQKTEIRSSDFALRKIGATKSRPVFSSAFIRACERMADYSAASPGAVLSA
                     MIPKTILDHLEETSASALEESEGAHEKLVMQAGDEERFSHYKSLIREEFARKRSVFFM
                     LPTVEDVRRMKPQLEKGIEEYSFALHGNLTPKKIRDAWDKARESSHPILVIATPQFLS
                     LPRSDLRTIIVEEEASRTYRGVSRPYIDGRLFAEFFAEESHARLILGDVLLQTETLWK
                     KMNDEYVDLIPLKFRSLSSAEARIVDMREEADEEKKRFRIFSDELERLILSTKENNER
                     LFLFGARKGYAGSTVCADCGEIVRCNNCGAPVAVYMLNADEGYFYCNKCGERRDAKER
                     CTVCDSWNLTMLGVGVERIEDELRKRFPDIQVFRLDKESVSTEKKALEIAEKFRETPG
                     SVLVGTELALIYMREIAENAAVVSIDTLFAVPDFRINEKLFHILLQIRSLAEKRFIIQ
                     TRNIGEKIFQYAVQGNLMDFYRDEIAMRKQFQYPPWSLFVKVSLEGPRASVEAEMRKL
                     QKFLEPFQADVLTSFDSPSAKRHQMHGLIKVKAQEWPNPALLEKLRALPPTFKIKVDP
                     DTLI"
     gene            complement(9036..10283)
                     /locus_tag="VHF05_00620"
     CDS             complement(9036..10283)
                     /locus_tag="VHF05_00620"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HVS79464.1"
                     /translation="MNSGFWKNLNKPVMVLAPMADVTDAAFRRIIAKYGRPDATWTEF
                     VSADGLFLGPAKEITEPMIRGELSREEFFKRAREGGKGTAIILDLMYDESERPIVAQF
                     FTSKPELMKKAAELAVVLGFDGIDINMGCPDDSINRQGAGAALIKTPELAQAVIRAAK
                     EGAAKGNASIPVSVKTRAGYNQEILETWVPMLLEEEPAVLTMHARIRKDMSKVPARWD
                     LVKRTREIRDAMGKETLIFGNGDAPDLLDARKKAEEAGADGVMLGRAIFGNPWLFANL
                     AHAKRGSQIERPVPISQDGAEMDGSQPSEINLEKASQSRNRADRHEHDAETSSREIDA
                     GLLYIPTTEEKLRVCVEHTKLFEELLGKHKNFSIMKKHYKAYIAGFNGAADLRGELMQ
                     TNTAGEIEEIINKFLKGTQKTAS"
     gene            complement(10687..>11601)
                     /locus_tag="VHF05_00625"
     CDS             complement(10687..>11601)
                     /locus_tag="VHF05_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS79465.1"
                     /translation="EEILRQRIVGQELAITAISDAMRRARAGVSNPNRPMGSFLFIGP
                     TGVGKTETTKALAEVFFGDENKITRFDMSEYSADDALTRLIGSFDTGKPGTFTTALRE
                     NPYGVLLLDEFEKANKEVHNLFLQVLDEGVFSDMTGKKVNARNLIIIATSNAGSDLIW
                     TLSQNGKDLAQAKGEVIDDIITKGIFKPELMNRFDGVILFHALGNDDLRSVARIQLER
                     LKKRLEERKLDLEINDALVEFLVKQGSDPKFGARPLNRAIQETVEAIIAKKLIDGSAK
                     PGAKISLTEADLAGNPVSASSEPVKNTN"
BASE COUNT         2684 a         3202 c         2983 g         2732 t
ORIGIN      
        1 gagctcgtgc acgacgatca aaacgacgat gattgctacg aaaatgagta ctgtcatgag
       61 tttatgagaa tttccaattt ttaattctca attttcaatg aattttcaat ttatcaataa
      121 gtaaactgca tgaatttgaa aattgcttca atgatccttg attgaaaatt agaaattgcg
      181 aattgaaaat ttattacgca gcaatttcct gctccttctt ctcggccatc tcttcgaggc
      241 gcttgttggc ctcgtcgacg attttctgca tctggtcctt cagcctgaat ttctcgtcct
      301 cgctcatgcc gccctccttc tcctttttct ggatgtcatc ccaggtcttt tcgcgctcgg
      361 agcggagcgc gacgcgcgcg tcctcgagct tctggcgcgc gaccttcacg agctggtcgc
      421 gccgttcggc ggtgagctcc ggaaacttca cgcgaaggcc cttctcatcg actgagacgc
      481 cgagcccgag atcgctttcc atgagcgcct tctccacgct tttcacctgc gacatatccc
      541 atggcgtgat gcggagcgtc cgggcgtcct cgatcgaaat gccgccaatt tggttcaacg
      601 gcatccgggt gccgtacgac tcgaccttca cgagatcgag gatcgccgga gtggcgcggc
      661 cggtgcggag gccctgatac tcctgcttga gccagtcctc gctttctttc actttctgct
      721 gaaacggtgc aaaattatat gccatacgaa ggggcggtaa ttagtactta gtaactagta
      781 aattagagga aaagcaattt aaactgcagg ataatcctag cagttttcaa aggaaaattc
      841 catgatttcc aatttattgc gcgcgcggca ttcatggtat agagtaggcg gagatcgctt
      901 actttggagg taagttatgg aaacacagga acgactttct tgctttttat gcaaaaggta
      961 tttgggaaac gttacattta cgcttgatga aatcattcat tccaaaccta tccgtcaatg
     1021 caggctgttc acaatacaac ccggcaaaca caacgaggtt cccagcacat tggagcaagt
     1081 aatcggcaga tcggcgcatc actatgtaac tttctcggaa cgaaaatcca tcggatcgct
     1141 tcacgaagga agaggcagaa cctttcaaaa cgagaacgat tccccgacgc tccacgtctt
     1201 cggagatcgg gtcggtcaga cgattctggt ttgcaactct tgttgcgaac acgctggcaa
     1261 cgaactgatc aatctgctcg atgcggaata cggctcgcgg ctcggacagc gggtgaatcg
     1321 gatctgcaga atattgcttg agaaaaacat agtgcggttc gatgccgaaa cgctcaagac
     1381 ttttgtcaag gagttcggaa gcagtgtact cagcgacagc tggaccgcta cgctcaaaga
     1441 attgaatgaa atcgcagttt acgacgatgt agcgagcctg ctcgactgtc ttcgtcctct
     1501 catcgatgag ttcattgcga gcgggcacga caatagtgcg cgcttgaaat tagtcagtta
     1561 acccaatcat cggttccgca tccgcggaac cgattgtttt tttaagtgca atcacccccg
     1621 gatattttca aaagaaaacc gcgtgtcgca agcgacacgc ggttgttttg aaaatgagca
     1681 aaattcagac tgacgctccg gccgcggccc cgagttctgg agagattgga atctcatgca
     1741 ctctcaagga aagtcctgcc gcaagaagcg cggtacagcg cggatgctca gtatgacagc
     1801 tcataagcat tccaatggga tccgttttgc aacgacgtgc aaacatctcg accgcttggg
     1861 atctcaggct ttgaatatcg caagctatcg tatcgagacc agatagtccg aacttcgtcc
     1921 tgatcaattc tgcaaaatat gccgtcagct cttcaggaat tgggcgatga gcgcgttcga
     1981 acgccgacac gatattttta atggttactt cggtttgaat ttccttcttt gccttaagaa
     2041 tccaatccga cgcttccgca aaacacactg ccggagcatc tctcattgcc ttgagggcga
     2101 gctcgacaat tgccgcctga ttacgcttgc gatccagttc gacaaacgag tcgcttatca
     2161 gagaatgaaa ttctctaagc attatgttga aaccgctgtc attcagggaa aagcgggaaa
     2221 tgagatcgtg tccttcctcg cataacctgc agatctggtc gaattcaagc gtccgccggc
     2281 ggtcgaagag aataacgaac ataatcaaaa tccgttcctt caccataccg aatgggatca
     2341 ggtcgatcat ctcatccact ccatccacag tacgagcaag ggtattcata tattccctgg
     2401 cctcgaagag tatgttggcg ccgacaagcc atactttgag cctgagcaaa tccaagttgt
     2461 tgttttccaa gatgtaaaaa aacctccgac aggtaaaata cactaaaaat agagtttagt
     2521 caattcgaac gccgtactag ggtgcgattg ccgcgatatg ttcgagaatg atttttacgc
     2581 ttggcgagcg gtcgagaaga tattgccttc ccttctcgat ttcttcgagc acgaaaatgt
     2641 cttcccttct cgcgctcgcg agcgagagat ttgctcgaac ttccacttcg agcgctgaga
     2701 cgaagaggag ggcgccactc ttgtccttct cagtcaccac aggcttcagg aattccccgc
     2761 gctccgcctg attcatcatg agaaactttt tggcatcctt ccgagcctcg gcggcggctt
     2821 ccgcgcgggc cggcacgatc tcgaggcgcg aacgcagggt cggcaggagg cgctcgcctg
     2881 acggcatgag aaagaagaaa tgagtacctt cggtcggctc ttccaatacc ttgaggagcg
     2941 aattctgcgc ttcaacggta atgccctcgg cgcgcacgac aaacaccttc cagccacccc
     3001 cgatcgcccg cctgagctcc ttatcggaaa gcacccttcc ctcgtcgatt cccatcgttt
     3061 cgaactcgcc tgtccagaaa tcgggattgc cgcgcgtgga aaatcccgct tcctcgagaa
     3121 attccacgag cgcggcgaaa ttctcggcga gcggcccctc gataagatag gcatggtgga
     3181 gattcttatt cttatgcgcg tcgcggatga tatccatata cgtatgagta tacaatatat
     3241 gagaataagt gatcagtata aggaacaaaa gggataattt agattccctg ctgggaatga
     3301 caataaaaaa taaggttggc ggaacgggat gtaattcaag gaacaccata tatatgaaaa
     3361 aaacagaact cgcaatttta gatgcaattc aaggcgcgag tgtgaagaag cgaaaacttg
     3421 gagcttctgc aaggccgcag gctgaccgag ccgaggcggg ctcgcgaaat tcgtcagtag
     3481 acgaatattc gtgaggcttg cgaccaaggt gtatgcatta tactccgcgg aagcaaacgc
     3541 aagcgagcaa cgcagaagtg cgctaaaatt gcgagttctg ctatcgtttg gaaatgatgg
     3601 tgcgtttata ggtatcgaga tgatcaagtg aaactcccgt tcccttcacc acgcagaaaa
     3661 gcgcctcgtc ggcgagcgtc gccttgacgc ccgtgcggcg atacatgagc tcgggaagat
     3721 tcctcaactg ggaagtgccg ccggtcatga tgatgccgcg atcgataatg tcggccgcga
     3781 gctcgggcgg cgtctcctga agcacgtctt tcaccgcctt taccatgtcg cgaagctcct
     3841 tcccgatcgc tttgacgatc tcgttcgtct tgatttcgac ggagcgcggc aggccggaga
     3901 tgaaatcgcg ccccttaatg gtcatggaaa gctcctcgtc gagcggaatc gccgagccca
     3961 ccttgatctt gatgtcttcc gcggtcttgt cgccaatgcc gagattgaag gttttcttga
     4021 tgtagtcggc gatcgcctgg tcgagccgat tgcccgcgac cttgaccgag gcggcggcga
     4081 caatgccgcc gagcgagatc accgcgacgt cggtcgtgcc gccgccgata tcgacgatca
     4141 tgtggccgac cgcttcctgg atcgggatgc ccgcgccgat cgcggcgagg atcggctcct
     4201 tcacgacata tgcgttcttc gctcccgcct tgatcgccgc ctcgatgacc gcgcgacgct
     4261 ccgtcgaggt gacgcccgca ggtacggaga caagcacgtc gggcttcacg aagttccagc
     4321 ggccgagcgc ccgctcgatg tagtagcgga gcatcgcttc cgtcacgcgg tagtcggcga
     4381 tgacgccgtc cttcatcggg cggtacgcga tgatgttgtc gggggtgcgg ccgatcatct
     4441 ccttcgcctc gaggccgatg gcgagaatgc ggttgtcctc ttcggagact gcgacgaccg
     4501 agggctcgtt caggacgatg ccgcggcccg gcacgaatac gagaatgttc gcggtgccga
     4561 ggtcgatgcc gatttttctg gagagaattc ccatgcttga gtgataatgc ggatttttag
     4621 tatagatatt attgacgaag agcgcccctt ttgcaaaccg cgctcctata tcaccacttt
     4681 ttagatgaag ctcaaaatac acgatggttg agagcttgaa tgggaattta cgcaaaaaaa
     4741 tgcggcgacc atgttgaaac tccggcggct tttccatagt atggatgggt atgatcccgt
     4801 tgtagaattt cgggttgcga attaaagagt aactgcggga aaagattagc aataaattat
     4861 agttgattat ccaaaaattc ggagactgtt cgcatcttcg cgaacgaccc gaaatttcac
     4921 aacaggatga aaaagaacaa aaaaatctgg ctcgtcgtcg gcctcctcgt catcgtgatc
     4981 gttttcgtca tcttgaatat tccttcgatc aagaagccgt tcggccaaac cgagacggga
     5041 aagatctccc tcgaggtccc gcttgaagga agcgaggtct acctcgacgg caagggatat
     5101 cagagcacca gcgtaaaaaa ccagactctc gacatacccg acgtcgcggc gaacaccccg
     5161 cacaccgttc tcgtcgccaa gaacggctac tggccatggg gcaagacggt gactgtcgag
     5221 gcgggcagga cgctcgatct caccgcattc aacggccctc aggccgaagt gacgacggac
     5281 gttgccgcgg gttcgaagga ttatgccgcc gcgaccgcgg cgctcgccac gaataccgga
     5341 accccaagca aggcaaccat gatcgtctcg aaggacggca gcgtcggcat ctgggtcgaa
     5401 ggcaacgcgc tcatggccga atggaagggc gaggacgatc ccgaccagtt cttctgcgtc
     5461 gagggcctct gcgacaagga tacgcaggtc ttcaagggca ccgctcccat tacccatctc
     5521 gccttctata aaaaccgcga tgacatcgtg ctcttctcct cgggcggagc gatctacgcg
     5581 atcgagttga accgcaacgg cacgcagaac ttccagcccg tcttcatggg caccaatccc
     5641 actttcgcgc cgtcgtccga cggaacggtt ctcttcgtga aggacggagg aaagatccag
     5701 gaggtgctgc tgtaaagtcg agagtaaata gtagtgagta gaaagaagaa tgtcctgcgg
     5761 cgcgtacgcg ccgcaggaca ttcttagatt ttgtgcatca tcgcacagat gaaccagatg
     5821 atgaacggtg ctgacattat ccaagtaccg agtgaagatt tttttccttc catgctgcat
     5881 ctcctataat tatttacctc atttttattt taaagtcaaa ttatcaaaat gcatgataaa
     5941 acaagaagct ccacataagc acagctccga tcaccacccc attaaacgca tgggtatggt
     6001 caatgatgtc caaaatagct ctgcgaggtt ttaataaacg agaatatacg ccggttaaaa
     6061 ttccgaattt cttttcaata ttcgatcctt caaccgtaca ttcgatatat aagtctctca
     6121 atcgcctctc aagcagccat gctccgataa taatcacaag cgctaaaagc ggaatgaaga
     6181 acaatgcagg ctgagcagta gaaagcacgt gcgtattatc gaacaattcc acataagctt
     6241 tccaattcac tttaaataca ttgccgaaaa aagctgtgat cgtttgcggc ggcgagctcc
     6301 tcactgtaat gttattaaac atatagttgt aagccccggc caagatcaac tgagttgaaa
     6361 cgacacctcc taagatcgcc cagcgattcg tatgcaatga tctcctgttc gctcccgctt
     6421 ctttgtacac ttcgatatgg aactgatctt gccgttcttt ttctttctgc agcgcttccg
     6481 caagttccct gtctcgccgc gcgaattcct cgcgagtggg cagaaggtac cctgtttcaa
     6541 atgccatttc gatttccttt caatgtccaa gctgggtaat ttaaaccata gaatctattt
     6601 tttgtacaca accctggcac cttcggacta aaagctataa gctaaaagct ataagcttat
     6661 gaaaatcctt tccgtctacc ccatttcgcg gggcatttcg aaagaaaagc tctcgtactt
     6721 ctcctccgaa gaccacgcgc tcggatcgat catctccgtg ccgctccgca agcgctccgt
     6781 ccccgccatc gtggtcggct ccgccgatgc cgaaacgcag aagaccgaaa tccgctcctc
     6841 cgacttcgct ttgcggaaaa tcggcgcgac gaagtcgcgt ccggtcttct cctccgcttt
     6901 catccgggcc tgcgagcgca tggccgacta ttccgccgcc tcccccggcg cggtgctctc
     6961 cgcgatgatc ccaaagacta tcctcgacca tcttgaagag acatccgcat ccgctctgga
     7021 agaaagcgag ggcgcccatg aaaagctcgt catgcaggca ggcgacgaag agcgcttctc
     7081 ccactacaag agcctcatcc gcgaggaatt cgcccgcaag cgctccgtct tcttcatgct
     7141 gccgaccgtc gaggacgtgc gccggatgaa gccccagctc gagaaaggca tcgaggaata
     7201 ttccttcgcc ctccatggca atctcactcc caagaagatc cgcgacgcat gggacaaggc
     7261 gcgcgaaagc tcccatccga tcctcgtcat cgccacgccg caattcctct cgcttccgcg
     7321 gagcgatctg cgaacgatca tcgtcgagga agaggcgtcc cgcacgtatc gcggcgtatc
     7381 gcggccgtat atcgacggca ggctcttcgc cgagttcttc gcggaagagt cgcatgcgcg
     7441 cctcattctc ggcgacgttc ttctccagac cgaaaccctc tggaaaaaaa tgaacgacga
     7501 atacgtcgat ctcattcctc tcaaattccg atcgctttcc tccgccgaag cgcgcatcgt
     7561 ggacatgcgg gaggaggcgg acgaggagaa gaagcgcttc cgcattttct ccgacgagct
     7621 cgaacggctc attctgtcca cgaaggaaaa taacgagcgg ctctttctct tcggcgcgcg
     7681 caagggctac gcaggttcga cagtctgcgc cgactgcggc gaaattgtcc gctgcaacaa
     7741 ctgcggcgct ccggtagccg tctacatgct caatgccgac gagggctact tctattgcaa
     7801 caaatgcggc gaacgccgcg acgccaagga acgctgtacg gtctgcgaca gctggaacct
     7861 cacgatgctc ggagtcggcg tcgagcgcat cgaggacgaa ttgcggaagc ggtttccgga
     7921 catccaggtc ttccggctcg acaaggaaag cgtctcgacg gagaaaaagg cgctcgagat
     7981 cgcggagaaa ttccgcgaaa cgcccggaag cgtcctcgtc ggcaccgagc tcgcgctcat
     8041 atatatgcgc gagatagcgg aaaacgcggc ggtcgtatcg atcgacacgc tcttcgccgt
     8101 ccccgacttc cgcatcaacg aaaagctctt ccacatcctc cttcagatcc gctcgctcgc
     8161 cgagaagcgc ttcatcatcc agacgcgcaa catcggcgag aagatcttcc aatacgccgt
     8221 ccaaggcaac cttatggact tctaccgcga cgagatcgcc atgcgcaagc agttccaata
     8281 tccaccgtgg agcctcttcg tgaaagtctc gctcgaaggg ccgcgagctt cggtcgaagc
     8341 ggaaatgcgc aagctccaga aattcctcga gccgttccag gcggatgttc ttacgtcgtt
     8401 cgattcgcct tccgcaaaac gccatcagat gcacgggctc atcaaagtga aagcgcagga
     8461 gtggccgaat ccggctctcc tcgaaaagct ccgggcgctt ccgccaactt tcaagatcaa
     8521 agtcgatccg gatacgctca tctagctgtg agcttgtagc tttcagctgc taggaaagtg
     8581 ggagaaactg cataaatgaa aaaggacgcc agtgttctgc aatgagaact gctgcgtcct
     8641 tatttccttt attgctgccg acaattgtcg gcgggggctc catctcagag cccctaaagg
     8701 cataccgtca ttttacctcg acggttacga ggaaggtttt accaggcgct tagcctggca
     8761 gcgctaatgc gctgacaaag taccggttcg atcgcgacag tgcagccgca tcggtatttt
     8821 tgaccatttc ctttctgaga aaatctcaag atggcattgt ttcggtgccg gcggtcggtt
     8881 cagtaactaa gaaaatgaag tgtcgtgctc ggaggtccgg tcatcaacta atccttttct
     8941 ctgaatttct ctactgcttt tattagcata tcattttatt ttataaaagt caagcacttc
     9001 tgtaatttta tgagtggtca gataagaaaa cggctttacg aggccgtttt ttgagttcct
     9061 ttcaagaact tatttatgat ttcctctatt tccccagcgg tattcgtctg catgagctct
     9121 ccccgcaggt ctgccgcccc attgaaaccc gcaatgtatg ccttgtagtg ctttttcatg
     9181 attgagaagt tcttgtgctt ccccagaagc tcttcgaaaa gcttggtgtg ctcgacgcac
     9241 accctaagct tctcttccgt ggtcggaatg tagagtaatc ccgcatcaat ttcgcgagag
     9301 gaagtttcgg catcgtgctc gtggcgatcg gcacgattcc ggctctgcga tgctttttca
     9361 agattaattt cactgggctg actaccatcc atttcagcac catcctgcga aatcggtacg
     9421 ggacgctcaa tttggctgcc acgcttcgca tgggccaaat tggcgaacag ccacggattg
     9481 ccgaagatcg cgcggccgag cattacgccg tccgctcccg cttcctccgc tttttttctc
     9541 gcatccaaaa gatcgggcgc gtcgccattg ccgaagataa gcgtttcctt ccccatcgcg
     9601 tcgcggatct cccgcgtgcg cttcaccaga tcccagcgcg cgggaacttt cgacatgtcc
     9661 ttcctgatgc gcgcgtgcat ggtgagaacc gccggctctt cttcgagaag catgggcacc
     9721 cacgtctcaa gtatttcctg attgtagccg gcgcgcgttt tcacggatac cggtatgctc
     9781 gcgttcccct ttgccgcacc ttcctttgcg gcgcggataa ccgcctgcgc gagttcggga
     9841 gtcttgatga gcgccgcacc cgctccctgc ctgttgatcg agtcgtctgg acagcccatg
     9901 ttgatatcga tgccgtcgaa gcccaggaca acggcaagct ccgccgcttt tttcatgagc
     9961 tccggctttg aagtgaagaa ttgcgcgacg attggcctct cgctttcgtc gtacatgaga
    10021 tcaagaatga tcgcggttcc cttcccgcct tccctggcgc gtttgaagaa ttcctcgcgc
    10081 gaaagctctc cgcgaatcat cggctccgtg atctctttcg cgggtccgag aaaaaggccg
    10141 tcggccgaaa cgaactcggt ccatgtcgcg tcgggcctgc cgtacttggc gatgatgcgg
    10201 cggaatgcgg cgtcggttac gtccgccatc ggcgcaagca ccatgacagg cttattcaaa
    10261 tttttccaga atcccgaatt catattaaaa tgccaaaaat aagggcggga ggaggaatgc
    10321 gcgtactctt agagcacgga ggtggatgtg cattcaactc aactcgccgc cccatctcga
    10381 ttcctggccc ggagacaaac tgtctccaac ccatctgcct gatcgagatt gtatgaagta
    10441 ctatagcatg cttttccgat ttgtacattt ttacacatgg tctggcaaag agtgcgacaa
    10501 aatgggttat taaaaggccg gctcctttcg ggagccggca attccttctc tgactttttg
    10561 gctacggtgg gtcagatcag ggccacgagc gcggatgtac gaacgtttgg aggtgacatc
    10621 gcgaaatttc ctttccttga gcgatcctac tgcctgataa gaatctaaca ccgctcagct
    10681 tttccgtcaa tttgtgtttt tcacaggttc gcttgaagca gaaacgggat tgccggccaa
    10741 atcagcttcc gtaagcgaaa tctttgcccc cggcttcgcg gagccgtcga tgagcttctt
    10801 cgcgataatc gcttccaccg tttcctggat ggcgcgattc aaaggccgcg cgccgaattt
    10861 cggatcgctt ccctgcttca ccaaaaattc gacaagcgcg tcgttgatct cgagatcgag
    10921 cttgcgctct tcgaggcgtt tcttcaggcg ctcgagctgg atgcgcgcga cggagcgcaa
    10981 gtcgtcatta ccgagcgcat ggaagagaat gacgccgtcg aagcggttca tgagctcggg
    11041 cttgaatatc cccttcgtga tgatgtcgtc gatgacttcc cctttcgcct gggcgaggtc
    11101 cttgccgttt tggctgagtg tccaaatgag atcgcttccg gcgttcgagg tggcgataat
    11161 gataagattg cgggcgttca ctttcttgcc ggtcatgtcg gagaagacgc cttcgtcgag
    11221 cacctggagg aaaaggttgt gcacttcctt gttcgccttc tcgaactcgt cgagaagaag
    11281 cacgccgtag gggttctcgc ggagggcagt cgtgaacgtg ccgggctttc cggtatcgaa
    11341 cgatccgata aggcgggtca gcgcgtcgtc agcgctgtac tccgacatgt cgaaacgggt
    11401 gattttgttt tcgtctccga agaatacctc ggcaagcgcc ttggtcgttt cggtctttcc
    11461 gacgccggtc ggcccaatga agaggaacga gcccatgggg cggttcggat tggagactcc
    11521 ggcgcgcgcg cggcgcatag cgtcggaaat ggcggtgatg gcaagctcct ggccgacgat
    11581 cctctgccgc aggatttcct c
//
LOCUS       DASZYS010000011        14056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_528440, whole genome shotgun sequence.
ACCESSION   DASZYS010000011 DASZYS010000000
VERSION     DASZYS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528440"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..1104)
                     /locus_tag="VHF05_00630"
     CDS             complement(400..1104)
                     /locus_tag="VHF05_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79466.1"
                     /translation="MLSLIHLTRVAEMAVVKRVLDDKRHLRDMDALVAFGKHAAILQK
                     GGKVVERHVAGCVQLKNLAHEFRVFAMHSNRLRARVVHIAKRRKPRIYSLPRFLAQTA
                     SSIGGELAHILIRHPEFNRHHQNIVVGVVRAVVRLNMANHALLQKPLHLSTINGISCE
                     AVNFPANNPLRVATRYSRHHIAEYGAAGHFRAPLFYKLLDDFQMFAPRERAQLCELRF
                     NGQNLFVLYIGAFASV"
     gene            complement(1877..1949)
                     /locus_tag="VHF05_00635"
     tRNA            complement(1877..1949)
                     /locus_tag="VHF05_00635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1914..1916),aa:Thr,seq:ggt)
     gene            complement(2063..2473)
                     /locus_tag="VHF05_00640"
     CDS             complement(2063..2473)
                     /locus_tag="VHF05_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79467.1"
                     /translation="MKSPDPLTLSPQELLKINLSLIRELCFCPDGPPAHPMRPWQTIE
                     NERRFYGNMLMARMRNELPKFARGDCVKPIRLSFSDHSGETMKFNPSAVFTVLKIIYY
                     SDIATRKCSWAILINVNNEKIRNLPFPEEWFEKA"
     gene            complement(2544..3440)
                     /locus_tag="VHF05_00645"
     CDS             complement(2544..3440)
                     /locus_tag="VHF05_00645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HVS79468.1"
                     /translation="MRKTFYAIVIGLLLFAGMGVLYSHMNPPCSKPLAYSIGSIDPRF
                     DITKDQFLKEVQSSESVWEKAAGRDLFQYDPNSSFKINLVYDDRQERTDEYEQIEDLL
                     DKSRGQFDDIDSQYNDLKNEYLAKRSAYDASSSSLDQDLAAYNKEVDDWNAKGGAPEP
                     EYNKLQTEKKSLDQRAAALESDRQTLNNLADQINALADQGSALADNYNDTVSTYNEKF
                     GTSTQFEQGVYNGKEIDIYQFKGMQDLELVIAHELGHALGLDHVNDKTAIMYYLIGDQ
                     NLADIQPTSADLAELKGVCKLK"
     gene            3553..4152
                     /locus_tag="VHF05_00650"
     CDS             3553..4152
                     /locus_tag="VHF05_00650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79469.1"
                     /translation="MNLSDRDSRRIREFYGEAFARHGVDPKSLHWINSYNQRRRFEVL
                     AAAGDLRGASILDVGCGLGDFLEFLKERGIDADYTGIDIVPQFIEAARSRFPDGKFLL
                     ENILEMVGEFDYVLASGAMSFRVEDNENYYFEVIKRMFDLSRRAAAFNMLDRRAHEET
                     DIYAVYDPREIALFCETIARRIDVAIDYMPDDFTVYMYK"
     gene            complement(4214..4762)
                     /locus_tag="VHF05_00655"
     CDS             complement(4214..4762)
                     /locus_tag="VHF05_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79470.1"
                     /translation="MEKISTKTLVIIAVIVVVILIVAFGLTSWMGTKQASQTPPQTAS
                     STAPAEDQAEASVRDVVTTFGSQLQKVSLLAPNAASTMEEFYGPYVSSDLLAKWEADP
                     SNAPGRQTSSPWPNNIQVTDVSKSGDNYIVRGQIVFMTSNEVEHGGVAGLQDVTMTVS
                     TASGTPVITDYTAGQMSSGGAS"
     gene            4982..6328
                     /locus_tag="VHF05_00660"
     CDS             4982..6328
                     /locus_tag="VHF05_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79471.1"
                     /translation="MKRFRGTDYEERSIENASRAFGYVYWPKLMDDRCYGDTMLGDLL
                     AGSAAAHPHGAEYVGHVQAMAKSIRDSIKALKRRHISCVRLGASWAEADTPGGLSWMK
                     WYFEEFSRAGFEILPCLNYTPARFATARIERMRREAEGDLEHLKKIAELQPLSNIPPE
                     PLDAFADYVRDFIRMFGKYLRPGSHIELWNEPNINTDWNPEIDTDGRMFVDMLAPAAQ
                     IVRESGFKVLLGGLTKFIETLDWLERHGPLGVLDHADAVGFHILRGTWSEKRPPQPLS
                     ERIAAIKATMRTFADRDIPVFLDEVGFATLDLKLEVPQEELEEIQAALFADTFLALAD
                     GSAAYAYWYSAEDKIVDSVRFITTGWEDILQYFFGDTDMEGNPKLLRTLLEQGGPIRV
                     LEYIESRNAWDRAYEVIDRGTPYDWSVEQLKSIVEQMKALAHERTLRTAPKKKEAA"
     gene            complement(6987..7610)
                     /locus_tag="VHF05_00665"
     CDS             complement(6987..7610)
                     /locus_tag="VHF05_00665"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HVS79472.1"
                     /translation="MAYVLGFFAADGTMIKNTRGAHFIEFHITDFDLLKEIQNLFSSN
                     HAITRSAPIGKNKAIYKLQLGSKEMYADLAALGFESRKSNILTFPHVPDELMPDFIRG
                     YFDGDGCVSFTKIYRKDRNMLDWSFTTRFTSGSKKFLLELHRILLESGLKGGYLYKKQ
                     RGFELVFSRRDSLALFYLIYNNGRCPIYLRRKKKIFIAALKKLGYAE"
     gene            complement(7785..7858)
                     /locus_tag="VHF05_00670"
     tRNA            complement(7785..7858)
                     /locus_tag="VHF05_00670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7822..7824),aa:Met,seq:cat)
     gene            complement(8435..9079)
                     /locus_tag="VHF05_00675"
     CDS             complement(8435..9079)
                     /locus_tag="VHF05_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79473.1"
                     /translation="MADEPKKEEAKGGIPSSGQALSQLGIVLAILIMAQSLLDRLFGA
                     GGANILHSPLFLRLEDFFNGFKVVSVIASIGLGAGVWYLVRGINEIRAAERAALYPEP
                     EAGGKEVIVNSKWQRVIDHLESDNASDWKLAIIEADILLDEMLDAMGYQGDTVGEKLK
                     QIERSDFVTIDYAWEAHKVRNMIAHEGADFTLTKKEAIRVIELYKKVFEEFFLI"
     gene            complement(9094..10524)
                     /gene="gatA"
                     /locus_tag="VHF05_00680"
     CDS             complement(9094..10524)
                     /gene="gatA"
                     /locus_tag="VHF05_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HVS79474.1"
                     /translation="MSLDITKLTIKSAAEGLRKKDFSACELAESYLERIEERNPERNA
                     YLEIFEDVRAQAEAAQKTIDSGAAPLLAGIPLAMKDNILIKGRRASSASKILEGYTAA
                     YDSTAAKKLKEAGAVFLGRTNMDEFAMGSSTENSAFGVVTNPHDVSRVSGGSSGGSAA
                     VVADSMALASLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLMAMASSLDQIGPFAK
                     TVSDAELIFDAIKGADSMDSTTLPQGAFSARPLKEKMRIGIPEGLLDIPGLDGDVRAN
                     FEASVAKLKAQGHEIVDVPLPYLKYSLAVYYVIVPAEVSSNLARFDGVKYGLHKDGAN
                     LIEDYFLTRGEGFGPEARRRILLGTYVLSSGYYDAYYNKAVAVRRLIEKDFAQAFEAV
                     DAIVTPTTPSPAFKIGEKANDPMAMYLADIFTVPANVGGICGISLPSGEAVRDGKNLP
                     LGFQILAGAGREDILFALGKKFLGEE"
     gene            complement(10514..10816)
                     /gene="gatC"
                     /locus_tag="VHF05_00685"
     CDS             complement(10514..10816)
                     /gene="gatC"
                     /locus_tag="VHF05_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVS79475.1"
                     /translation="MIEPQTIEKLAELARIKVSREEVEGLRAEIEPILDYVNQIQDAV
                     GAGEIEPEAGAVRNVMRADDHPHESGIHSAELLEEAPAREGEYFKVKKILGGSDVA"
     gene            complement(11156..13183)
                     /gene="ligA"
                     /locus_tag="VHF05_00690"
     CDS             complement(11156..13183)
                     /gene="ligA"
                     /locus_tag="VHF05_00690"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVS79476.1"
                     /translation="MNDIPERKKAKERAEKLRETIEKHRRLYHVEDKTEMSPEALDSL
                     KYELAKLEEAYPELITPDSPTQRVAGEPLKQFKKVTHKVPQWSFNDAFTEEDILEFDA
                     RTKRFLKAKLGRDIQPGYVTELKIDGLKIVLEFSKGNLETAATRGNGKVGEDVTMNIR
                     TIESVPLRLSREADLIVEGEAYMKKSRFAEINREQEKKGLPIFANPRNFAAGTIRQLD
                     PKMVAERKLDMFVYDLSTADFPLPETQFEELKLLQSLGFRVNPNFKLHKDIGGVIEFW
                     KHWQGRKESQDYFFDGIVVKVNEREYQEALGYTGKAPRFGIAFKFAAEQVTTVVEDII
                     LQVGRTGVLTPVAVLRPVSVAGSTVSRATLHNEDEIRRLDVRIGDTVILQKAGDVIPD
                     IVSVVKEMRTGKEKPFEFPERVEACGGDGRIERVPGQAAWRCVVKNSFAQQQRKLYHF
                     ASKTAFDIDHLGPKVIDQLLDAGLIASYPDIFTLKKGDVLSLPRFGEKSVENLMASIE
                     AARKTTLPRFLIGLSIPQVGEETAYDLAGEFGSLEALMNAKYEELERIEGVGPVVANS
                     IVEWFKDKENRAVVRRLARLVKIEAAPRASGAKGATLAGKTFVLTGTLETMGRDEAKE
                     KIRALGGEVSGSVSKKTDFVVAGEEAGSKLDKAEELGVKVISEEEFLKMIK"
     gene            13291..13908
                     /locus_tag="VHF05_00695"
     CDS             13291..13908
                     /locus_tag="VHF05_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVS79477.1"
                     /translation="MHTKRGFTLIEMLVVLAIIVFMVTAVLANTHGITLEARAKAAAS
                     TVLDLIKDTRNRSVAVKQFTTGTFPSYGLDFDLSRPQQVIVYADCIADDNADGIVDNK
                     DTFNYISGGGSCTGGSLVSTNKLDTQTKIGAIRAVFPVSGGSIRTVSESRVDILFLRP
                     EPTIWIWTATDGVLTAGHVEVDIEDTTGKYIRTVSLYSTGEASMK"
BASE COUNT         3097 a         4040 c         3690 g         3229 t
ORIGIN      
        1 gtatgaagag gcggaaacgg ggcgagggag gccgcgccga gtccgcgagg cgcgggcggt
       61 caggaagtgg agaaatgggt tcgagccgag tacaatagag actcaaaaat caaactcaaa
      121 tccctttcgc cggattcgga atgcgcggcg cgaagcgccg cgtaggggga aatgggcaaa
      181 ccgcgcgctt cgcgcgcgtg atggaggttc agaaaaatac aaatccgaaa actttttgga
      241 ggggaggatt ttttggaggg tacatgcaga aggcagaaaa gcatttgcgg tgtgcttgcc
      301 ccacccaccc ttgcgcgcac cagtctttcc gcctttcgcc gttttgagga gacattataa
      361 aacggcggag gcggctcccg cgatatgacg aaatactacc tatacgctcg caaaagcacc
      421 gatgtagagg acaaacaggt tttgtccatt gaagcgcaac tcgcagagtt gcgcgcgctc
      481 gcgcggcgcg aacatttgga aatcgtcgag gagtttatag aaaagcggag cgcgaaaatg
      541 cccggccgcc ccgtattcgg cgatatgatg gcgcgaatag cgcgtggcga cgcgcaaggg
      601 attatttgct ggaaaattga ccgcctcgca cgaaatcccg ttgatggtgg acaggtgcaa
      661 tggcttctgc aacagggcgt ggttcgccat attcagacgc acgaccgctc gcactacccc
      721 aacgacaatg ttttgatgat gtcggttgaa ctcgggatgg cgaatgagta tgtgcgccaa
      781 ctcgccgcca atactgctcg cggtctgcgc caaaaagcgc ggcaaggaat atatccgggg
      841 cttgcgccgt ttggctatgt gaacgacccg cgcacgaaga cgattgctgt gcatcgcaaa
      901 aacgcgaaac tcgtgcgcga gatttttgag ttgtacgcat ccggcaacat gacgctcgac
      961 gaccttgccg cccttttgca aaatcgcggc gtgctttcca aaggcaacaa gcgcatccat
     1021 atctcgcaga tgtcgtttat catccaaaac ccgttttact acggccattt ccgctacgcg
     1081 ggtgaggtgt atgagggaaa gcacgagcca cttatttcaa aatcgctcta tgacaaagca
     1141 aacgcggttt tgaagaagcg cgggagaact cccaccatca aaaccgaccc tgcgccattg
     1201 tgcagcctcc tgcgatgcgg cttttgcggc atgggcatca cggccgaagt gaaggagaag
     1261 catcagaaaa acggcaatgt ccatcactac acttactatc gctgtacgcg caaaaacaag
     1321 gcggcgaggt gcaaagaagc gccgatgcga agcgaacttt tagagtcgca actttcggcg
     1381 cttcttgccg agtacgcgat gcctcaagaa tggacattgc cgcttttgaa gaaactagac
     1441 gaggaagccg caagtgcttc taaaacggcg gcggaggccg ttcaaggtct gcgggacaag
     1501 gttgaggaca tatcgcgcca aatatcgcgc cttacggact tgtatgtggc cgaggacttg
     1561 gagcgcgagg attacttgtc ccgccgccgc gctcttatga gcgagcgccg gacgattgag
     1621 gagcaaatcg tccgtcttga acgagccccc gccgtttggg ttgaacctgt gcgggaatgg
     1681 atttcggacg cttcgcgtct tgctgaaata gcaaaatcaa aagatctccc ctccaaaaaa
     1741 tcctccctcc aaaaagtttt cggattgaac ctctctatcc acgcgcgcga agcgcgcggt
     1801 ttgcccattt ccccctacgc ggcgcttcgc gccgcgcatt ccgaatccgg cgaaagggat
     1861 ttgagtttga tttttgagcc aagaccgagg atcgaactcg ggacctcccc cttaccatgg
     1921 gggtgctcta ccaactgagc tatcttggca tattccttat ttcctacgaa cttgcgacat
     1981 cataatataa cgtccgcagt ttttcaaatt ttaaaaagac cgttaacttt ccagttagcg
     2041 gccttctcgc atccgcagaa cctcatgctt tttcgaacca ttcctccgga aaaggcaggt
     2101 tgcggatttt ctcattattg acgttgatca atattgccca ggagcatttt cttgtagcaa
     2161 tatcgctata gtaaataatt ttcaatacag tgaatactgc cgatggattg aatttcatgg
     2221 tttcgccgct atggtctgaa aagctcaagc gaatcggctt tacgcaatcg ccgcgggcga
     2281 atttcggcag ttcattgcgc atgcgcgcca tcagcatatt tccataaaaa cgcctttcat
     2341 tttcaatcgt ttgccagggg cgcataggat gcgccggcgg accgtcggga caaaaacaca
     2401 gttccctgat aagcgaaaga tttattttca gaagctcctg cgggctcaaa gttaacggat
     2461 ccggactttt cacttttcta cctccctcaa aaagaatcct ctgattttta ttgtaagaac
     2521 aacctgaaca attgcaagtc aggctacttc agtttgcaga cacccttcag ctcggcgagg
     2581 tcggccgagg tcggctggat atctgcgaga ttctggtcgc cgatgagata gtacatgatc
     2641 gccgtcttgt cgttcacgtg atcgaggccg agcgcatggc cgagctcgtg cgctatcacg
     2701 agctcgagat cctgcatgcc cttgaactgg tatatgtcga tttccttgcc gttgtagacg
     2761 ccctgctcga actgcgtcga cgtgccgaat ttctcgttgt atgtggagac ggtgtcgttg
     2821 taattgtcgg cgagcgcgct tccttggtcg gcaagcgcat tgatctgatc ggcgagatta
     2881 ttgagggtct gccgatcgct ttcaagcgcc gcggcgcgct gatcgaggct tttcttctcc
     2941 gtctgcagct tattgtattc cggctccggc gcgccgcctt tcgcgttcca atcgtcgacc
     3001 tccttgttgt acgcggcaag atcctggtcg agcgatgagg acgaggcgtc atacgcgctg
     3061 cgcttcgcga ggtattcgtt cttcagatca ttgtattggc tgtcgatatc gtcgaactgc
     3121 ccgcgcgact tgtcgagcag gtcctcgatc tgctcgtatt cgtcggtgcg ctcctgccgg
     3181 tcgtcgtaga cgagatttat cttgaacgac gaattcggat catactggaa caggtcgcgg
     3241 cccgcggctt tctcccacac gctttcgctc gactggacct ccttgaggaa ctgatccttg
     3301 gtgatgtcga agcgcggatc gatagagccg atcgaatacg cgagcggctt cgaacacggc
     3361 gggttcatgt gcgaatagag cacgcccatg cccgcaaaga gaagcaggcc tatgacaatc
     3421 gcgtagaaag tctttcgcat tttcccattt taccatatcg acccttcccg aatctcggga
     3481 gaaaccccgg ccgccttcga aatgttgatg gggcttcacg gattccttat cggccgatta
     3541 tacttttaac ctatgaacct cagcgatcgc gactccaggc gcatcaggga attttacggc
     3601 gaggcgttcg cccgccacgg cgtcgatccg aagagcctcc attggatcaa ttcgtacaac
     3661 cagcggagga gattcgaggt gcttgcggcc gcgggcgacc ttcggggagc gagcatcctc
     3721 gacgtgggct gcggcctcgg cgacttcctg gaattcctca aagagcgcgg catcgacgcg
     3781 gactataccg gaatcgatat cgtgccgcag ttcatcgaag cggcgcggag ccggtttccg
     3841 gacggaaaat tccttctgga aaatatcttg gaaatggtgg gcgagttcga ttacgtgctc
     3901 gcctcgggag ctatgagctt cagggtcgaa gataatgaaa actattattt cgaagtaatc
     3961 aaacgcatgt tcgatctctc gcggcgggcg gccgcgttca acatgctcga tcgccgggcc
     4021 cacgaggaga cggatatcta cgccgtctac gacccgcggg aaatcgcgct tttctgcgag
     4081 acaatcgcgc gcaggatcga tgtcgccatc gactatatgc ccgacgattt caccgtctat
     4141 atgtataaat aaaaatcgcc cgtgagatgg cggccttgcc tgtctcacga gcgatttttt
     4201 atggcgggaa aatttatgac gcgccgcccg agctcatctg gcccgcggtg tagtcggtga
     4261 tcaccggcgt tccgctcgcg gtcgaaacgg tcatcgtcac gtcctggagg ccggcgacgc
     4321 cgccgtgctc gacttcgttc gacgtcatga agacgatctg gccgcggacg atgtagttgt
     4381 cgccgctctt tgatacgtcg gtaacctgga tgttattcgg ccatgggctc gacgtctgcc
     4441 tgccgggagc gttcgaagga tccgcttccc acttcgcgag aaggtctgag gaaacgtacg
     4501 gaccatagaa ctcttccatg gtgcttgcgg cgttcggcgc gagaagcgaa accttctgaa
     4561 gctgggagcc gaaagtggtt acaacgtcgc ggaccgaagc ctcggcctgg tcttccgcgg
     4621 gagcggtcga cgatgcggtt tggggcggcg tctgggacgc ttgtttggta cccatccacg
     4681 aggtcaggcc gaacgccaca atgagaatga cgacgacgat tacggcgata ataacgagtg
     4741 tttttgtgga aattttttcc atgattacga agtgttttta atgctggtaa gccaatgcac
     4801 tatatctgga tattgattat atcacttgcg ggaatgatat ttcattgaca tcgactgatg
     4861 agattgaccc aatgtttctt cacttttctc acatcacggc catataacct ctacatagac
     4921 gatggaatgc cggcattcca tgctgttctt aggtttggga gaataataac gaggagaaac
     4981 aatgaagaga tttcgaggaa cagactacga agaaaggagt atcgaaaacg cgagcagggc
     5041 gttcggctat gtctattggc cgaagctcat ggacgaccgc tgctatggag acacgatgct
     5101 cggcgatctt ctcgccggct ctgcggcggc gcatccgcac ggtgcggaat acgtcgggca
     5161 tgttcaggca atggcgaaaa gcatacgcga ctcgatcaag gcgcttaagc gccgccatat
     5221 cagctgcgtc cggctgggcg catcgtgggc ggaagccgac acgccgggcg gtctatcatg
     5281 gatgaaatgg tatttcgagg aattcagcag agccggattc gagatacttc cctgcctcaa
     5341 ctacactccc gcccgattcg caacggcgcg catcgaaaga atgcgccgcg aagctgaggg
     5401 ggatcttgag catctcaaga aaattgcgga gttgcagccg ctctcgaata tcccgccgga
     5461 gcccctcgat gcgttcgcgg actacgtccg ggatttcatc aggatgttcg gaaaatatct
     5521 ccgccccggt tcgcacatcg aactttggaa cgagccgaat atcaatactg actggaatcc
     5581 ggagatcgac acggacggca ggatgttcgt cgacatgctc gcgcctgcgg cacaaatcgt
     5641 ccgcgagagc ggattcaagg tgcttctcgg tggcctcacg aaatttatcg agacacttga
     5701 ttggctcgag cggcacggcc cgctcggcgt tctcgatcac gcggacgcgg tcggattcca
     5761 catcctgcgc ggcacatgga gcgaaaagcg ccctccgcag ccgctttcgg agcgcatcgc
     5821 cgccatcaaa gcgacaatgc gcacattcgc cgatcgcgac attcccgtct tcctcgatga
     5881 agtgggattc gcgacgctcg acctcaagct cgaagtgccc caggaagagc tcgaagaaat
     5941 tcaagcggcg ctctttgccg ataccttcct cgcgctcgcg gacggctcgg cggcatacgc
     6001 ctactggtac tcagccgaag acaagatcgt cgattcggtg cgcttcatca cgacgggctg
     6061 ggaggacatc ctccagtact ttttcggcga taccgatatg gagggaaatc cgaagctcct
     6121 ccgcacgctc ctcgaacaag gcggcccgat acgagttctc gagtacatcg aatcgcgcaa
     6181 cgcatgggat cgcgcatacg aagtgatcga tcgcggcacc ccctacgact ggtcggtcga
     6241 acagctgaaa tcgatcgtcg aacaaatgaa agcgctggcg cacgaacgaa cgctccgcac
     6301 ggctcccaaa aagaaagaag cggcataacc gcttcttttt ctttgcgggg gtgatggctt
     6361 ggtcacgaat attttgctcg ccgtcgctcg caaaatttct cgaccccggc tcggtatttt
     6421 cttctgctga agaaaatata cctgcgccct tcaagtcctt gaaatgtttc gcatttcatc
     6481 ggccacgaaa aaatcccctt tcagggattt ttggcgcggc cgaccggact tgagggtcaa
     6541 gtttctaaac tcgctccgct cgttaagaac ttcctccccg agctcgcctg ccgcaaagcg
     6601 gcatggctcc gcttgttcaa gtccggacgg aagcgaagca gttcgcttcc ttcggccgct
     6661 tgaacgcaaa aaatcctgga aaaactccag aattttttgg ttctgcgcgg ccgaccggac
     6721 ttgaaccggc aacctctcgc gtgcactgat ccacaatttt catagtggcg tggactatat
     6781 cttcaccatg agccgatgct tttaggcgct tgggtatcta gtctctacgg cgctctcatc
     6841 aaattgatga gttcccacgg tgttcgcatg tttgcttatg caaatttagc cttcaccgtt
     6901 atccccaaga gtttcaattt gccgtttcgg caaaaagctg caattactta caggcgagtg
     6961 ctctaaccag ttgagctacg accgcattat tctgcatatc ccaatttttt caaggccgca
     7021 atgaaaattt tcttctttcg cctcaaataa ataggacacc gcccattatt atatattaag
     7081 tagaaaagcg caagactgtc ccgccgacta aaaacgagct cgaaaccacg ttgtttttta
     7141 tacaggtagc cacccttgag gcctgattcc agcaatattc tatgcagctc caataaaaac
     7201 tttttgctcc ccgacgtgaa acgggtcgta aacgaccagt cgagcatatt gcggtctttg
     7261 cgatatattt tggtaaaact tacgcaaccg tctccgtcga aataccctcg tataaaatcc
     7321 ggcatcaatt catcgggaac atgcggaaaa gtgagaatgt tgctttttct tgattcaaaa
     7381 ccgagtgccg ccaggtcagc atacatttcc ttacttccaa gttggagttt ataaatcgct
     7441 ttattcttcc cgatcggggc gcttcgcgta atcgcatgat ttgaggaaaa aagattttgt
     7501 atctccttta ataaatcaaa atctgtaatg tgaaattcta taaaatgcgc tcctcgtgtg
     7561 ttttttatca tggttccgtc tgccgcgaag aaacccagta cgtatgccat ttcagggttc
     7621 cagaatttga agaactttct gttgagagtg cgtgcaatcg gcatttctaa ataataccac
     7681 gtgacatgat attactctat acaatcttta ataaaattat ttacctatgt ttaagtgccg
     7741 actttgcctt cctcccaggc agtccatact gcctgttcac cgggtgtcgg cgggtgggat
     7801 cgaaccaccg accttgggct tatgagtccc acgctctaac caactgagct acgccgacat
     7861 tacacgtaaa ttatccatat tctcgtgcat ttagcaaaca aaaacaggca tatgcctgtt
     7921 tttgtctcct tgttgcggtg tcccgatttg gtcacgagtt actaagtctt ttgtcactat
     7981 cgtgccaaaa gtccaagtac tctcgacccc ggctcgcccg ccgcacagcg gcatggctcc
     8041 gccctgcaaa tcgggccgaa agcgaagcag ttcgctttct actccttaca agtatgaaaa
     8101 tacccgccga aagcgggtat tttcatactt gttgcggtgt cccgatttgc acgggagcct
     8161 gcgggttatg agcccaccga ggtactgctc ctccacaccg ctataaactc cttagaggcg
     8221 ttaattatag cgcttcgaac aaacattgca agcacaacat tgccgaccgt caaacctcca
     8281 aatctggatg cagttcaagg tgaggaagcg agtgctcgca gcttttgcaa ggccggagcc
     8341 cgacggggcg aggcggggtc gcagaagttt ttagcagaaa actatccgtg accgagtttt
     8401 gaatataatg agaaaatgcg ccggatttgg aggtttatat gaggaagaac tcctcgaaga
     8461 ctttcttata cagctcgatc acccgaatcg cctccttttt ggtgagagtg aaatcggcgc
     8521 cttcatgggc aatcatattg cgaaccttat gggcttccca tgcataatcg atcgtaacga
     8581 agtcgctcct ctcgatctgc ttcagcttct cccccaccgt gtcgccctgg tagcccatcg
     8641 catcgagcat ttcgtcgaga agaatgtccg cttcgatgat ggcgagcttc cagtcgcttg
     8701 cgttgtcgga ttcgagatgg tcgatgaccc gctgccattt gctgttgacg attacctcct
     8761 tgcccccggc ttcaggttcg gggtagagcg ccgcccgttc ggccgcgcgg atctcattga
     8821 ttccccggac aagataccag acgcccgcgc ccaaaccgat cgaagcgatt accgagacga
     8881 ctttgaatcc gttgaaaaaa tcctccagac gcagaaagag aggactgtgg aggatgttcg
     8941 cgcctcccgc gccgaatagc ctgtcgagaa gcgactgcgc catgatcaaa atcgccaaga
     9001 cgatgccgag ctggctcaaa gcctgccccg acgaaggtat gcctcctttc gcctcttctt
     9061 ttttcggttc atcagccatc ctaatagtga aatttattcc tccccgagga atttcttgcc
     9121 gagcgcgaag agaatgtctt ccctgcccgc tccggcaagg atctggaatc cgagcggaag
     9181 attcttgccg tcgcgcactg cttctccgga aggaagcgag attccgcaga tgccgccgac
     9241 attcgcggga acggtgaata tgtccgcgag atacatcgcc atcgggtcgt tcgccttttc
     9301 tccgatcttg aacgcgggcg acggggtcgt cggggtcacg atcgcgtcga cagcttcgaa
     9361 ggcctgcgca aaatcctttt cgataagccg ccgcaccgcc accgctttat tatagtacgc
     9421 atcgtaatat ccggacgaga ggacgtacgt gccgagaaga atcctgcgcc gcgcttccgg
     9481 gccaaatcct tcgccgcgcg taagaaaata atcctcgatg agattcgcgc catctttgtg
     9541 aaggccatac ttcacgccgt cgaagcgagc cagattggac gacacttccg ccggcacgat
     9601 gacgtaatag accgcgaggg aatatttgag atacggaagc ggcacgtcga cgatttcgtg
     9661 cccctgagcc ttgagcttcg cgacggatgc ttcgaaattg gcgcgcacgt caccgtcgag
     9721 cccgggaatg tcgagaagcc cctcgggaat gccgattctc attttttcct tgagcggccg
     9781 agcggagaac gccccctggg gaagcgtagt gctgtccatg ctgtccgcgc ctttgatcgc
     9841 gtcaaaaatg agctcggcgt cggacacggt ctttgcaaac ggaccgatct gatccagcga
     9901 agacgccatg gccatgagcc cgtagcggct cacccttcca taggtgggtt tgagccccac
     9961 gacgccgcaa aaacttgccg gctggcggat cgatccgccg gtatccgagc cgagcgacgc
    10021 gagcgccatg ctgtcggcga ccacggccgc cgaacctccc gacgacccgc ccgatacgcg
    10081 agacacatca tgcgggttcg tcacgacacc gaacgcggag ttctcggtgg agcttcccat
    10141 ggcaaactca tccatattgg tgcggccaag aaataccgca cccgcttcct tgagcttctt
    10201 cgcggccgtc gaatcatagg cggcagtgta cccttcgaga attttcgaag cggacgacgc
    10261 gcgacggccc ttgatgagaa tattgtcctt cattgcaagc ggaatgcctg caagaagcgg
    10321 agcggcgccc gagtcgatcg ttttctgcgc cgcctccgcc tgcgcacgca cgtcctcgaa
    10381 aatctccaaa tatgcattgc gctcgggatt ccgctcctcg atgcgctcaa ggtaactctc
    10441 cgcgagctcg cacgccgaaa aatccttctt gcgaaggccc tccgctgcgc tttttatcgt
    10501 aagcttggta atatcaagcg acatcgcttc ctcctaaaat tttcttaact ttgaaatact
    10561 cgccttcgcg cgccggcgcc tcctccagaa gctccgcgct gtggatgccg ctctcgtgcg
    10621 gatgatcatc ggcgcgcatg acattccgca cggcgcccgc ttcaggttcg atctcgcccg
    10681 caccaaccgc atcctggatc tggttcacgt agtcgagaat aggctcgatt tccgcgcgca
    10741 agccttctac ctcctcgcga gagaccttga tgcgcgccaa ttcggcgagt ttttctatcg
    10801 tttgcggctc tatcatgggc tttatagtat cacgttacca ttctggagga acccggcaac
    10861 ccaaagtaat tgacggagag agacgcgcat gcttcactac agtcagaatg atttacaacg
    10921 accccgtacc acctcaaccg cctcgagctc cgctcaagcc ggttaaagcg gtcgttgcca
    10981 cgtcgcaaca gcacccagtc cctccccctc ttcccaagcg cgccccgcat cgcgcgccaa
    11041 cgccggaagt ggccaatcgc cgtctcgcct tgcgcaagaa agtgcgggcg aaaccatgga
    11101 tgaaattcca tccgctctaa tcagcagccc gagattgatc tcgggctgcc ttttcttatt
    11161 tgatcatctt caaaaattcc tcctcggaaa tcactttcac tccgagctcc tccgccttgt
    11221 cgagcttcga tccggcttct tccccggcga ccacgaaatc cgtttttttc gagacggacc
    11281 ccgagacttc gcctccgagc gccctgatct tctccttcgc ttcgtcgcga cccatcgttt
    11341 caagcgttcc ggtgaggacg aaggtcttgc ccgcgagcgt tgcccctttg gcacccgagg
    11401 ccctgggcgc cgcctcgatt ttcacgagcc gcgcgagcct tcgcacgaca gcgcggtttt
    11461 ccttatcctt gaaccactcc acgatcgaat tcgccaccac aggccctacc ccttctatcc
    11521 tttcaagctc ctcgtacttt gcgttcatga gagcctcgag cgagccgaat tctccggcca
    11581 aatcgtacgc ggtttcctct ccgacctgcg gaatggagag gccgatcagg aaccgcggca
    11641 acgtcgtctt ccgagcggcc tcgatggatg ccataaggtt ctcgacggac ttctcgccga
    11701 agcgcgggag gctcagcaca tcgccttttt tcaaagtgaa aatatccgga tacgaggcga
    11761 taagcccggc atcgagaagt tggtcgatca ccttcgggcc gaggtggtcg atgtcgaagg
    11821 ccgttttcga cgcgaaatgg tagagcttgc gctgctgctg ggcgaacgaa tttttcacca
    11881 cgcaccgcca cgccgcctgg ccgggaacgc gctcgatgcg cccgtcgccg ccgcacgcct
    11941 ccacgcgctc cggaaattcg aacggctttt cctttcccgt ccgcatctcc ttcaccaccg
    12001 acacgatgtc gggaatgacg tctcccgctt tctggagaat caccgtgtcg ccgatgcgca
    12061 cgtcgagccg cctgatctcg tcctcgttgt ggagcgtggc gcgcgagacc gtcgagcccg
    12121 cgacggaaac cggcctcaaa accgcgaccg gcgtcagcac ccccgtgcga ccgacctgga
    12181 gaatgatgtc ttcgacgacc gtcgtcacct gctcggccgc gaacttaaag gcgatgccga
    12241 agcgcggcgc ctttccggtg tagcccaacg cctcctgata ttcgcgctcg ttcaccttca
    12301 ccacgatacc atcgaagaaa tagtcttggc tttctttcct gccctgccag tgcttccaaa
    12361 actcgattac gccgccgata tccttatgaa gcttgaaatt gggattcacc ctgaagccga
    12421 gactctgcag gagcttcaat tcttcgaact gcgtctcggg aagcggaaaa tcggcggtag
    12481 aaagatcgta gacgaacatg tcgagcttgc gctcggcgac cattttcggg tcgagctggc
    12541 ggatggttcc cgccgcgaaa tttcgcggat tggcgaaaat cggcaatccc ttcttctcct
    12601 gctcgcgatt aatctccgca aaacggctct ttttcatata cgcctcgcct tcgacgatga
    12661 gatcagcctc gcgggatagg cgaagcggca ctgactcgat cgtgcgaatg ttcatcgtca
    12721 catcctctcc cactttgccg ttgccgcgcg tcgcggccgt ctccaaattg cctttcgaga
    12781 attccagaac gattttaagc ccgtcgatct tgagctccgt cacgtatccg ggctgtatgt
    12841 ctcggccgag cttggccttg agaaagcgct tcgttcgcgc gtcgaactca aggatgtctt
    12901 cctcagtgaa tgcatcgttg aaggaccatt gcggcacctt atgcgtcact ttcttgaact
    12961 gcttcagcgg ctcgcccgcg acgcgctggg tcggcgaatc gggcgtaatg agctccggat
    13021 acgcctcttc gagcttggcg agctcgtatt tcaagctgtc gagcgcctcg ggagacatct
    13081 cggtcttgtc ctccacatgg tagagcctgc ggtgcttctc gatcgtctcc ctgagcttct
    13141 ccgcgcgctc cttcgctttc ttgcgttccg gtatgtcgtt cattgcctta atcattccaa
    13201 aaaatggcgg aaaaacaagt cgaacaagcc gaaagttatc aacgttttta attgatgcgc
    13261 gtccgtatca aggctataat tttcccatag atgcacacga aacggggctt cacgctgatc
    13321 gagatgctgg tagtacttgc catcatcgtt ttcatggtta cggccgtgct tgcgaacacg
    13381 cacggcatta cccttgaggc acgcgcgaaa gccgccgcaa gcacggtcct cgacctcatc
    13441 aaggacactc gcaaccggtc ggtcgccgtc aagcagttca cgacgggaac gttccccagc
    13501 tacggcctcg atttcgatct gtcccgcccc cagcaagtca tcgtctatgc cgactgcatc
    13561 gccgacgaca acgccgacgg catcgtcgac aacaaggata ccttcaacta catttccggc
    13621 ggcggaagct gcactggcgg cagcctcgta tcgaccaaca agctcgatac ccagaccaag
    13681 atcggagcga tccgcgcggt atttccggta agcggcggct cgatccgcac cgtatcggaa
    13741 agccgggtcg atatcctgtt cctccgaccc gaaccgacga tctggatctg gacggcgacc
    13801 gacggcgtcc tcaccgcggg gcacgtggag gtcgacatag aggatacgac cggcaaatat
    13861 atccgcaccg tgagcctcta cagcacggga gaggcttcga tgaaatagca tgaaaaagca
    13921 ggagctcaaa aaattcttcg cacgactgcc ggcaggcgcc tcccgccacg gctttaccca
    13981 agcatcaaca tttggtgctg ggtttaccgt gatggagctc atcgtctcct tcaccattct
    14041 ctccctctcg ctcgcc
//
LOCUS       DASZYS010000012         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_146532, whole genome shotgun sequence.
ACCESSION   DASZYS010000012 DASZYS010000000
VERSION     DASZYS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146532"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VHF05_00700"
     CDS             complement(<1..2123)
                     /locus_tag="VHF05_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVS79478.1"
                     /translation="MQIPYDPKKIEPKWQKKWQDEKLYEADDVSPSPKFYSLIEFPYP
                     SGAGLHTGHIRSNTAMDIISRKRRMEGWNVLYPIGWDAFGLPTENFAIKTGKDPAVVT
                     KENTDNFRRQLQSLGFSFDWSREINTTDPAYYKWTQWIFLQMYKKGLAYKAKMTINWC
                     PKDKIGLANEEVIDGKCERCGTQVEKREKEQWMLAITKYADRLDKDLDTVDFQERIKI
                     QQRNWIGRSEGAEIEFPISNFQFPNNEKTKVKVFTTRPDTLFGATYLVLAPEYPFIEN
                     LKFKIKNWSEVELYIKQAKQKSEIDRTAETKDKTGVRLDGVTAVNPVTNEEIPMFVAD
                     YVLAHYGTGAIMAVPAHDARDFEFAKKFGLPIQEVVMERLVDAKNPHVPGKEIVFRDA
                     VIAAVRDPKTGKFLILKWKKQPWTTFVMGGIEKGEDAATAAAREILEETGYKNVKVVK
                     TLREAQSEFYAAHKGVNRVAHTRNLILELVNDEREKISDEESEIHEIAWMSLDEIRNA
                     GMTHSEMPIFYEGFESIEIPFTGRGILTNSGKFDGMSSEDAAKAIVEAVGGKIVTTYK
                     LRDWVFSRQRYWGEPIPLIHCDNCGWQPVPEEDLPVRLPKVEKYEPTDDGESPLSKIS
                     EWVNVKCPNCGGDAKRETDTMPQWAGSSWYYLRYADPHNAEAFADASKLKKWLPVDWY
                     NGGMEHTTLHLLYSRFWHKFLFDQGL"
     gene            complement(2207..>3202)
                     /locus_tag="VHF05_00705"
     CDS             complement(2207..>3202)
                     /locus_tag="VHF05_00705"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HVS79479.1"
                     /translation="TLYITAFSLLALLFQVINHAFPDALSAGGYFGAYGNPYSAAVRW
                     SIASVVVTFPIFWFVSWELNREYAIAPEKKSLGVRRWLVYFTLFIAGITIMIDLIVLL
                     NTFLGGEITARFVLKVLAVLLVAGLIFGYYIYDLKKYPSGPSKALRNFMLTATLIILA
                     SIAGGFAVMGSPMSQRNAQFDSRRVSDLMGIQSEIVSYWQRKGALPQNLDALNDPIAS
                     YAAPADPQTGASYEYKQTGALSFELCATFRTASNPAANEVPPMYPGASNDVWTHGAGR
                     QCFDRTIDPQLYPPVNQTGQDTGIVPRGATQTNGNLNGPDYFPNGGPGTNLPEQE"
BASE COUNT          638 a          897 c          890 g          777 t
ORIGIN      
        1 agcccctgat cgaagaggaa cttgtgccag aagcgcgaat agaggagatg aagcgtcgtg
       61 tgttccatgc cgccgttgta ccagtcgacg ggaagccatt ttttaagctt cgaagcgtcc
      121 gcgaaagcct ccgcattgtg cggatcggca tagcgaagat agtaccagga agagcctgcc
      181 cactgcggca tggtatcggt ttcacgcttg gcatcgccgc cgcagttcgg gcacttcacg
      241 ttaacccact cggaaatctt cgagagcggc gactcgccgt cgtcggtcgg ctcgtacttc
      301 tccactttcg gaagccgtac gggcagatct tcctccggca ccggctgcca gccgcaatta
      361 tcgcaatgaa taagcgggat cggctcgccc cagtagcgct ggcgcgagaa gacccagtcg
      421 cgaagcttgt aggtggtgac gattttgccg cccaccgcct cgacgatcgc cttcgcggcg
      481 tcttccgagc tcatgccgtc gaactttccg gagttcgtga gaatgccgcg gccggtgaac
      541 ggaatttcga tactttcgaa tccctcgtag aagatcggca tctcgctgtg ggtcataccg
      601 gcattcctga tctcatcaag ggacatccag gcgatctcat ggatttcgga ttcctcgtcg
      661 gaaatcttct cgcgctcatc gtttacgagc tcgagaatga gattgcgcgt gtgcgccacg
      721 cgattgactc ccttgtgggc ggcatagaat tccgactgcg cttcgcgcaa agtcttcacg
      781 actttcacgt ttttgtatcc ggtctcctcg agaatctcgc gcgccgcggc agttgccgcg
      841 tcctcgccct tctcgatgcc tcccatgacg aacgtcgtcc acggctgctt cttccatttg
      901 agaatgagga attttccggt cttcggatcg cgcactgcgg cgatgaccgc atcgcggaag
      961 acgatttcct ttccgggaac gtgagggttt ttcgcgtcga cgagccgctc catcacaacc
     1021 tcttgtatag gaagcccgaa ctttttcgcg aattcgaaat cgcgcgcgtc gtgcgccggc
     1081 acggccatga tcgctccggt gccgtaatgc gcgagcacgt agtcggcgac gaacatcggg
     1141 atctcctcgt tcgttacggg atttaccgca gtcactccgt cgagccttac tcccgtttta
     1201 tcctttgttt ctgccgtccg atcaatttct gatttttgtt tcgcttgttt gatatacagc
     1261 tcgacttcgc tccaattttt aattttaaat tttaaatttt caataaacgg atattcgggt
     1321 gcaagtacga ggtaggttgc gccgaaaagc gtgtccggcc tcgtcgtaaa gacttttacc
     1381 tttgttttct cattatttgg aaattggaaa ttggaaattg gaaattctat ttcagctccc
     1441 tccgagcggc cgatccagtt cctctgctgg atttttatcc tctcctggaa atcgacggtg
     1501 tcgagatcct tgtccagcct gtcggcgtat ttggtaatgg cgagcatcca ttgctccttc
     1561 tcgcgcttct ccacctgcgt gccgcagcgc tcgcatttgc catcgatcac ttcctcgttc
     1621 gcgaggccga tcttatcctt cgggcaccaa ttgatagtca ttttcgcctt gtaggcgagg
     1681 cccttcttgt acatctggag gaaaatccat tgcgtccatt tgtaatacgc gggatcggtc
     1741 gtattgattt cgcgcgacca gtcgaatgaa aagccaagcg actggagctg acgcctgaag
     1801 ttgtcggtat tttccttcgt gacgaccgcc ggatcctttc cggttttaat tgcgaagttt
     1861 tcagtgggca ggccgaacgc gtcccagcct atcggataca agacgttcca tccttccatc
     1921 cggcgcttgc gcgagatgat gtccattgcc gtattgctcc tgatatgccc cgtatggagg
     1981 cccgctcccg acggatacgg aaattcgatc agactgtaga acttcgggct cggcgagaca
     2041 tcgtcagctt cgtagagctt ctcgtcctgc cattttttct gccacttcgg ctcgatcttc
     2101 ttggggtcgt aagggatttg catggagatg tattacaagc aaatcttaca agacaaaacc
     2161 ggctttgcca aatgcagcgc cggttcgcca taatctgaat attttgctat tcctgctccg
     2221 gcaagttcgt tcctggcccg ccgttcggga aataatcggg gccgttgaga ttgccgttgg
     2281 tctgcgtggc gccgcgcggc acgattccag tgtcctgccc cgtctggttt accggaggat
     2341 acaattgagg atcgatggtg cggtcgaagc actgccgtcc cgctccatgc gtccagacgt
     2401 cgttcgaggc gccggggtac atcggcggca cttcgttcgc cgccggattg ctcgccgtgc
     2461 ggaacgtcgc gcaaagctcg aacgaaagcg cgcccgtctg cttgtattca tagctcgctc
     2521 ctgtttgcgg atcggcaggc gcggcgtagc tcgcgatcgg atcgttcaag gcatcgaggt
     2581 tctgcgggag cgcgcccttt ctctgccagt acgagacgat ctcggattga atgcccataa
     2641 ggtcgctcac gcggcggctg tcgaactgcg cgttgcgctg ggacatggga gagcccatga
     2701 ccgcgaagcc gccggcgatc gacgcgagaa tgataagcgt cgccgtcagc atgaaattcc
     2761 gaagagcttt ggacgggccg ctcggatatt tcttgagatc atagatgtaa tagccgaaga
     2821 taagccctgc gacgagaagc acagcaagca ccttgaggac gaagcgcgcg gtgatctccc
     2881 cgccgaggaa ggtgtttaag agcacgataa gatcgatcat gatcgtgatg ccggcgataa
     2941 agagcgtgaa gtagacgagc cagcggcgca cgccaaggga ttttttctca ggcgctatcg
     3001 catattcgcg attgagctcc catgacacga accaaaatat ggggaaggtc acgacgaccg
     3061 acgctatcga ccagcggact gcggccgagt acggattgcc gtacgcaccg aagtagccgc
     3121 ccgccgacaa ggcgtccgga aaggcgtgat tgatgacctg gaacaggagc gccaagaggc
     3181 tgaaggccgt aatgtagaga gt
//
LOCUS       DASZYS010000013         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_18736, whole genome shotgun sequence.
ACCESSION   DASZYS010000013 DASZYS010000000
VERSION     DASZYS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18736"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VHF05_00710"
     CDS             complement(<1..502)
                     /locus_tag="VHF05_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79480.1"
                     /translation="MPTQLMTALRPGFPSLTRRQRVIGLLLFFAYIGATILGIQSFVA
                     PAILTPTSGIALAGLVIGGIELWPMVFLAAVAAYMFYGSTWVTILFMSVGVTLQAVIG
                     AYALKKLSFNPSFERLRDMLSFMVVAFVSSTIVPSLGFLSRALNHLILNRPSITSSVT
                     WGSWWTG"
     gene            complement(630..1532)
                     /locus_tag="VHF05_00715"
     CDS             complement(630..1532)
                     /locus_tag="VHF05_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVS79481.1"
                     /translation="MATEEAEGARKALIIWIIFCAAAACAFAFFYMRSSIELARKTSD
                     WNASELELENQIGNLNQSLSVATTENASLTSAYAAEKERNDSFEGRISGIADTVGTLQ
                     KLSETDPELLAKYSKVYFLNENYIPSSLSPIPSQYLEDPSRKLQIHSGALPFFARLMG
                     AAEDDNIDLKVASAYRSFGQQAALKESYEVTYGSGANAFSADQGYSEHQLGTTLDFIT
                     SKQGGSLSGFDKTPAYAWLLDNAYKYGFILSYPEGNSYYEYEPWHWRFVGIKLATDLH
                     NEKKNFYDLDQRDIDAYLVNIFDN"
     gene            1659..2705
                     /locus_tag="VHF05_00720"
     CDS             1659..2705
                     /locus_tag="VHF05_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVS79482.1"
                     /translation="MKIAQIAPIIERVPPKKYGGTERVVHALTEELVRRGHEVTLFAS
                     GDSETTAELFSVYPRALREARLTDLYGMNVYTTMNIGVAYERQHEFDIIHDHNGYASL
                     PAANMSDTPVVMTYHGPFSPEVRTVFQTLRKPNVVTISWAQAKYAPNINHAGTVYNGL
                     DMDAYPFSATHEGYLLFVGRISEEKGVHNAIHAAQYLNLPLIIAAKLDQADLQYFNNH
                     IKPFLSDEIRWIGEVDVAERNALMSKAMVMLHPVGWPEPFGLTMIEAMACGSPVIAFN
                     RGSIPEVVKDGETGFVVNDVEEMIDAIGRLNEIDRAKCREYALTNFNHRKMTDGYLEI
                     YERLIAEKKNGEAK"
     gene            2776..3696
                     /locus_tag="VHF05_00725"
     CDS             2776..3696
                     /locus_tag="VHF05_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HVS79483.1"
                     /translation="MNKKLVFPVMILVLAAIVAGVSRIARGGGGIFALFEPHGAIAAQ
                     ESHIIIIAVALMFAVAIPTFIFLLYVVAKYRDDNTKAPYRPEERGGKKAAAFLWGVPA
                     LLILVIAVMNWHTTHELDPYKPLDSTTAPITVQVVSLRWKWLFIYPEQHIATVNFLEI
                     PDNTPVNLELTSDAPMNSFWVPQLAGQMYSMAGMSTQLHFIADEPGDFQGRAAEINGP
                     GFAGMSFTVRAASADDFAHWVDTVRNSHKALTAEEYAKLALPSEYVPAAYYASVPDDM
                     YNMIMGKYMSPTTVAMASDGGGAMQGMKMR"
     gene            3834..5822
                     /locus_tag="VHF05_00730"
     CDS             3834..5822
                     /locus_tag="VHF05_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVS79484.1"
                     /translation="MFGRLTLDALPHEWFTIGGTASIIGGAIVIAALLTYYKRWKWLW
                     REWLTSTDPKKIGIMYIVVAMVMLFRGALDAGMIWLQQSLSSGMSHGYLDPYHFQQIF
                     TAHGVIMVFFVTTGFLFGMMNFILPLQIGARDVAYPLLNTLSFWLYVAGAILANMFFM
                     FGGEFAAAGWLSVPPLSELAYSPGVGVDYWIWTLQVSGLGSLLSGINLLVTTLKMRAP
                     GMTLRKMPMFAWASVWSNLLVITVFPVLTMTLFLLTLDRTLGMHFFTSDMGGNPMMYI
                     NLIWSWGHPEVYILMIPQFGMFSEIVSVLSGKKLFGRTSLMAAAAAITILSGFVWLHH
                     FFTMGAGADVNAFFGIMTGIIAIPTAVQIFNWIGTMWRGRIRLATPMYWFLGFVLTFT
                     IGGIAGVLLGIPGADFELHNSLFLVAHFHTMLIGGALFGIFAGIAYWFPKAAGWKLNE
                     RVGRYAFWCWLVGLITSFTPLYILGFMGATRRLDHYDASLGWQGLFIVAACGFVILGL
                     GALLQLVQVGVSFRQRRKNRDTTGDPWGGRTLEWAVSSPPPFYNFAVIPRVRSAEPLL
                     DMHDDAASAGTAAVKYEDIEMPKNTPMGIYLSGLAFLIGFSMVWHIWWLAILSVAGVI
                     VCIVRRTLDEETEYVIPAAEVERMEREGLTMYDPNPYA"
BASE COUNT         1237 a         1649 c         1639 g         1303 t
ORIGIN      
        1 cgcccgtcca ccaggacccc caggtgacgg atgacgtgat cgacggccgg ttaagaatca
       61 gatgattcaa tgcgcgggat aaaaatccca gcgagggcac gatcgtcgac gagacaaatg
      121 cgacaaccat gaacgagagc atgtcgcgga ggcgttcgaa cgacggattg aatgaaagct
      181 tcttcagcgc ataagcgccg atgacagcct gaagcgtgac gcccaccgac atgaaaagga
      241 tcgtaaccca cgttgagccg tagaacatat aggccgcgac ggcagcaagg aaaaccatcg
      301 gccagagctc gattccgccg atgacgaggc ccgccagggc gatgcccgag gtgggagtga
      361 ggatcgccgg agccacgaac gactggatgc cgagaatcgt cgcgcctata tatgcgaaaa
      421 agagcagcag gccgataacg cgctggcggc gcgtgaggga gggaaaacca ggcctcaaag
      481 ctgtcatcaa ttgggtgggc atacggctaa atacggcaat ttctatctat cctctgcgac
      541 gtacatgaag atttaatgag tataatcccc aaggctgtcc ccgggtcaag agagctttcg
      601 tccgcgcttc ctccggcggc tctatctagc tagttgtcaa aaatattgac gaggtaggcg
      661 tcgatatcgc gctggtcgag atcgtagaaa ttcttctttt cgttgtggag atcggtggcg
      721 agttttatgc cgacgaacct ccaatgccac ggctcgtatt cgtaatatga gtttccttcc
      781 gggtaggaga ggatgaagcc gtatttgtac gcattgtcga gaagccatgc gtaggcgggg
      841 gtcttgtcga agccgctaag gctgccgccc tgcttggagg tgatgaagtc gagcgtcgtg
      901 ccgagctgat gctccgaata tccctgatcg gcggaaaacg cgttcgcacc ggatccgtag
      961 gtaacctcgt agctttcctt aagcgccgcc tgctgtccga aggagcggta ggcggatgcg
     1021 actttgagat caatattgtc atcctccgcg gcgcccataa ggcgggcgaa aaacggaagg
     1081 gcgcccgaat gtatctggag ctttctcgac ggatcctcga gatattgcga gggaatgggc
     1141 gagagggagg acggaatgta attctcgttc aggaaataca ccttcgaata ttttgcgaga
     1201 agctcggggt cggtttcgga aagcttttgg agcgtcccga cagtatcagc gatgccgctt
     1261 atccgtcctt cgaatgaatc gttgcgctct ttctccgcgg catacgccga agtgagcgaa
     1321 gcgttttccg tcgtcgcgac ggagagcgac tgattgagat tgccgatctg attttcaagc
     1381 tcaagctccg aggcattcca atcgctcgtt ttgcgggcaa gttcgatcga gctcctcata
     1441 tagaagaacg cgaacgcgca tgcggccgcc gcgcaaaaaa taatccatat aatgagcgct
     1501 ttgcgcgctc cttccgcctc ttccgtagcc attgtgatac agtatagcac ggcagagttc
     1561 gcggaaacga ccgcgatttt agcaggaaaa ataacggctg agttcgaggc tgtcaacctc
     1621 agcataactt cattcagccg gagtatagat aaagggccat gaaaatagca caaatcgcac
     1681 cgattattga acgcgttcct ccgaaaaagt acggaggcac cgaaagggtg gttcacgcat
     1741 tgaccgagga gctggtccgc aggggccatg aagtgacgct ttttgcgagc ggcgattcag
     1801 aaacgacagc ggagcttttc tcagtttatc cgagggcgct ccgcgaagcg cgcctaacag
     1861 acctctatgg catgaatgtg tatacgacga tgaacatcgg cgttgcctac gagcgccagc
     1921 atgagttcga cattatccac gaccacaacg gctacgcctc gcttcctgcc gcgaacatgt
     1981 ccgacactcc cgtcgtcatg acgtatcacg gcccgttcag ccccgaagtc cggacggtct
     2041 tccagacgct ccgcaagccg aacgtcgtca ccatttcctg ggcccaggcg aagtacgcgc
     2101 cgaacatcaa ccacgcgggc actgtctaca atgggctcga tatggacgcc tatccgtttt
     2161 ccgcgacgca cgagggctac cttctcttcg tcggaaggat ttccgaggag aagggcgtcc
     2221 acaacgccat tcatgcggcg cagtacctca atcttccgct tatcatcgcg gcgaagctcg
     2281 atcaggcgga ccttcagtat ttcaacaatc atatcaagcc gttcctctcc gacgagatcc
     2341 gctggatcgg cgaagtcgac gtggccgagc gcaacgcgct catgtccaag gcgatggtga
     2401 tgctccatcc ggtaggctgg ccggagccat tcggcctcac aatgatcgaa gcgatggcct
     2461 gcggctcgcc ggtcatcgcg ttcaaccgcg gctccattcc ggaagtggta aaggacggcg
     2521 aaaccggctt cgtggtgaac gacgtcgagg aaatgatcga cgctatcggc agattgaatg
     2581 agatcgaccg cgccaagtgc cgcgaatacg cgctcacgaa cttcaaccat cggaaaatga
     2641 ccgacggcta tctcgagata tacgaacgcc tcatcgcgga gaagaaaaat ggcgaagcga
     2701 agtaggtttt tttcacaagc tttcccgaag aacgggcctc ttggtggtat actaactcca
     2761 taaggccttc aactgatgaa taagaagctt gtgttcccag ttatgattct cgttctcgcg
     2821 gcaatcgtcg cgggcgtgag ccgcatcgcg cgcggaggcg gcggcatctt cgcgcttttt
     2881 gagccgcatg gcgccatcgc cgcgcaggaa tcgcacatca tcattatcgc ggtcgcgctc
     2941 atgttcgcgg tcgccattcc tacgttcatt tttctgctct acgtggttgc gaaataccgc
     3001 gacgacaaca cgaaagcgcc gtaccggccg gaagaaaggg gagggaagaa agccgccgcc
     3061 tttttgtggg gcgttccggc gcttctcatc ctcgttattg ccgtcatgaa ctggcatacg
     3121 acgcacgagc tcgatccgta caagccgctc gactcgacaa cggcgccgat taccgtgcag
     3181 gtcgtttcct tgaggtggaa gtggctcttc atttaccccg aacagcatat cgccacggtg
     3241 aatttcctcg aaattcccga caacacgccg gtcaatctcg aactcacgtc ggacgcgccg
     3301 atgaattcat tctgggtacc ccagctcgcg gggcagatgt attcgatggc gggaatgtcg
     3361 acacagctcc atttcatcgc cgatgagcct ggtgatttcc agggccgtgc ggcggaaatc
     3421 aacggcccgg gctttgccgg catgtcgttt acggtgcgcg ccgcgtctgc cgatgatttc
     3481 gcgcactggg tcgacacggt gcggaattcg cacaaggcgc tcacggcgga agagtacgcg
     3541 aagcttgctc tgccgagcga gtacgtgccg gcggcgtatt acgcgtccgt gccggacgat
     3601 atgtataata tgatcatggg aaaatacatg agcccgacca ctgtcgccat ggcatcggac
     3661 ggaggcggcg cgatgcaggg aatgaaaatg aggtgataaa attttcaatt tgcaattctt
     3721 aattttcaat caatttatca atattcaatt ttcaaggaat tacagatttt catttgcgac
     3781 attgaagcat tgaaaattca ttgaaaattg caaattgaaa atttaaaact atcatgttcg
     3841 gccgacttac attagacgcc ttgcctcacg aatggtttac catcggagga acggcctcga
     3901 tcatcggagg cgcgatcgtc atcgctgccc ttctcacgta ctacaagcga tggaagtggc
     3961 tgtggcgcga gtggctcacg tcgaccgatc cgaagaagat cggcatcatg tacatcgtcg
     4021 tcgccatggt gatgctcttc cgcggcgcgc tcgatgcggg aatgatctgg ctccagcagt
     4081 cgctctcgtc gggcatgtcg cacggctatc tcgatccgta ccatttccag cagatcttca
     4141 cggcgcacgg cgtgatcatg gtcttctttg tcacgaccgg cttcctcttc ggcatgatga
     4201 atttcatcct gccgcttcag atcggcgcgc gcgacgtggc ctatccgctc ttgaacacgc
     4261 tctcgttctg gctctacgtc gcgggggcga tcctcgccaa tatgtttttc atgttcggcg
     4321 gggaattcgc cgcggcaggc tggctttcgg tgccgccgct ctccgagctt gcgtacagcc
     4381 cgggcgtcgg cgtcgactat tggatatgga cgctccaggt ttcgggtctc ggcagcctcc
     4441 tttccggcat caaccttctc gttacgacgc tcaagatgcg cgctcccggc atgacgctcc
     4501 gcaagatgcc gatgttcgcc tgggcgagcg tctggagcaa ccttctcgtc attaccgttt
     4561 ttcccgttct cacgatgacg ctcttcctcc tgacgctcga ccgcacgctc ggcatgcatt
     4621 tcttcacctc ggacatgggc ggcaatccca tgatgtacat caatctcatc tggtcgtggg
     4681 gccatcctga ggtttacatc ctcatgattc cgcaattcgg catgttctcg gagatcgtaa
     4741 gcgtgctgag cggcaagaag ctcttcggcc gcacgtcgct catggccgcc gccgccgcca
     4801 tcacgatcct ttccggattt gtctggctcc accatttctt tacgatgggc gcgggcgccg
     4861 acgtgaacgc cttcttcggc atcatgaccg gcattatcgc cattccgacg gcggtgcaga
     4921 tcttcaactg gatcggcacg atgtggcgcg gccgcatccg cctcgcgacg ccgatgtact
     4981 ggttcttggg cttcgtcctt acgttcacta tcggcggcat tgccggcgtg cttctcggca
     5041 ttccgggcgc cgatttcgag ctccacaaca gccttttcct cgtagctcac ttccatacca
     5101 tgcttattgg cggcgcgctc ttcggaattt tcgccggcat cgcatactgg ttcccgaaag
     5161 ccgcgggctg gaagctcaat gagcgcgtcg gccgctacgc gttctggtgc tggcttgtcg
     5221 gccttattac ctcgtttact ccgctttata tcttaggatt catgggcgcg acgcgccgcc
     5281 tcgaccacta tgacgcgagt ctcggctggc aagggctttt catcgtggcg gcatgcggct
     5341 tcgtcatttt gggactcggc gctctgctcc agctcgttca agtgggcgta agttttcgtc
     5401 agcgcagaaa gaaccgcgac acgacaggcg atccgtgggg aggccgcacg ctcgaatggg
     5461 ccgtttcctc gcctccgccg ttctacaatt tcgcagtaat tccgcgtgtc agatcggccg
     5521 aaccgcttct cgatatgcat gatgatgctg cctccgccgg gacggccgcc gtaaagtatg
     5581 aagacatcga aatgccgaag aacactccca tgggcatcta tctttccggt cttgcgtttt
     5641 tgatcggctt ctcgatggtg tggcacatct ggtggcttgc gatcctaagt gtcgcgggcg
     5701 tcatcgtctg catcgtgcgg cgcacgctcg atgaggaaac cgagtacgtc attcccgccg
     5761 ccgaagtcga acggatggag agggaaggct taacgatgta cgatccgaac ccgtatgcat
     5821 aacgaaac
//
LOCUS       DASZYS010000014         9633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_397923, whole genome shotgun sequence.
ACCESSION   DASZYS010000014 DASZYS010000000
VERSION     DASZYS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397923"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..792
                     /gene="rpmE"
                     /locus_tag="VHF05_00735"
     CDS             535..792
                     /gene="rpmE"
                     /locus_tag="VHF05_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HVS79485.1"
                     /translation="MKAEIHPEYYPDAKVYCACGATYTIGSTKKEIHVEICSACHPFY
                     TGESKTIDAAGRVERFKARKAAAAAAPKKEKKAKVRKSEEE"
     gene            980..1957
                     /locus_tag="VHF05_00740"
     CDS             980..1957
                     /locus_tag="VHF05_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HVS79486.1"
                     /translation="MEDQEKYKSNPKTTYLAQMWQKLHDDEMEVLKMSENDPSMRDLA
                     ADELKNLQEQQSALLRQMDDILKSEAEEEEFPNEIVLEVRAGAGGEEAALFAEELANM
                     YQKYAASKGWGWKVVDESPSALGGYKEASFEVRGKDVYKILRFETGVHRVQRVPATEK
                     QGRVHTSTASVAILPIRKKVKFILNPAELEFETSRGGGKGGQNVNKVETAVRVIHKPT
                     GLWVRATAERSQGANREMALAILSAKLEALKEEEEAKKFASERKGQIGTGDRSEKIRT
                     YNFPQNRVTDHRIKESWHNIEQIMLGQIDDIVEALQNATSFSETDDDEE"
     gene            1959..2678
                     /locus_tag="VHF05_00745"
     CDS             1959..2678
                     /locus_tag="VHF05_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVS79487.1"
                     /translation="MEREAEWLLRDKYDGVETPEYHKDLERLAAGEPLAYTIGWTPFL
                     NTKIFLDSRPLIPRPETEYWVGNAIAEAEKNSKPLKILDLCAGSGCIGVAVLKALPNS
                     TVDFIEIDPAHHPLIAKNIRENSIDESRTRILGGDLFENAGTGYDLILSNPPYIDPAL
                     QRTDGSVLNHEPHLALFGGERGMEIIERIIADGPAHLALGGTLYIEHEPEQAEAIAKL
                     LPGSVQLKDQFGLIRVTRYKK"
     gene            2761..3258
                     /locus_tag="VHF05_00750"
     CDS             2761..3258
                     /locus_tag="VHF05_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79488.1"
                     /translation="MNAKKLFLIIFIVLFLILSAGFVFWNKQTSKPAINISGSQQNSE
                     NVIGEESTTTTSFLGYYLTNNSVAGKPKECSIFVIPKSSDPLFDFFSEMVQQGNTVNS
                     IDSKGDLFLNVDLANVSTVDKARVVSSTESNPIILDVQKKRMEGKDAGPCTSFVDILS
                     VEVPS"
     gene            3459..4169
                     /gene="rpsB"
                     /locus_tag="VHF05_00755"
     CDS             3459..4169
                     /gene="rpsB"
                     /locus_tag="VHF05_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HVS79489.1"
                     /translation="MSEATTTNPKVEELFKAGAHFGFVRSRRHPSAKPFIYGTKNRIE
                     IFDLEKTAEELDKAASYIKEIAKGNGTVLFVGGKSEAREAIKSAAESLDMPYVAGRWI
                     GGTLTNFSEIKKRVAKLEDLTNQKQKGELAKYTKKERLLIDREIENLHRFFSGLANLK
                     DKPAVMFVIDAKHEHIAVAEAKTMGVPVVALCGSDCNFGEVDYAIPGNDSSVPSVKYF
                     VERIAAAYKEGKSEKAGA"
     gene            4305..4892
                     /gene="tsf"
                     /locus_tag="VHF05_00760"
     CDS             4305..4892
                     /gene="tsf"
                     /locus_tag="VHF05_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639006.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HVS79490.1"
                     /translation="MATITTEMIKELRDKTGGVSVMQCKKALEEAGGDMEKALVIIQR
                     ASKSAALKKADRTLGAGVVQSYIHSTGNIGSLVLLSCETDFVSRNEEFKTLARDIAMH
                     VAAADPQYLKTEDIPEADRKKAEEVLAEEIGDKPEDMKAKILEGKLASYFQEKVLLEQ
                     PFIKDPERTIGNLVESAIQKFGEKVELTRFVRFTV"
     gene            5040..5465
                     /locus_tag="VHF05_00765"
     CDS             5040..5465
                     /locus_tag="VHF05_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79491.1"
                     /translation="MQTIFIVSASIIFAMIALRALELKFQRPIILAHLSWPADKRLRV
                     YYKKFRHYLDERIKTRIDAFAERLKERANAYLEQARLGGAEYALSLQESLRGKKALSR
                     GSVSFFLLNISDYNIEKKKGPDPALIAESMPLEGTEALQ"
     gene            5545..5617
                     /locus_tag="VHF05_00770"
     tRNA            5545..5617
                     /locus_tag="VHF05_00770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5578..5580,aa:Thr,seq:tgt)
     gene            complement(5633..6013)
                     /locus_tag="VHF05_00775"
     CDS             complement(5633..6013)
                     /locus_tag="VHF05_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79492.1"
                     /translation="MTPPAAHIPLSIRLKEYVSRLGRQAWKFFNVGDGAQYEWHALRA
                     RDFYDRSVTLDSLGETAFDSLPSLFAKKAAAPVRDHTPRIQPKERWLHALHRDDIRGI
                     PSPFQTALKSPFDEHAAKPLTATH"
     gene            complement(6507..7214)
                     /locus_tag="VHF05_00780"
     CDS             complement(6507..7214)
                     /locus_tag="VHF05_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79493.1"
                     /translation="MDKFGAKKYWFAFAITAVIFVLAFFASNYLNQKRISEMRSIQDQ
                     ISIDLQSSETQFDLLGQVSCEDLGNSALSKELNSIAEQLSYMESNRSPDDPEVITLKE
                     YYSILEIKDYILMQKIADKCDVKPIFILYFYSNNGDCPECEKTGYVLTQMRQDYPELR
                     IYSFDYNLNLSALRTLISLYGVEPTLPAMILNGKVYYGFQSTDDIERIIPEIKALRAS
                     TTEATSTTATTSSTTKK"
     gene            complement(7365..8603)
                     /locus_tag="VHF05_00785"
     CDS             complement(7365..8603)
                     /locus_tag="VHF05_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVS79494.1"
                     /translation="MATFTTAIVYIFLFLSLYFEVFLLITYFERQEEVDEAGGMRTLT
                     SHPTVTIMVPVWNEEKTVSGTVNSLLALDYPKDKLSVLVVDDGSTDGTWEVLQSFTGN
                     PQVKIAKKPNGGKYTALNYGLERSDSEFVGCLDADSFVDPQALNEIMPFFDDPKVMSV
                     VPSIKVHNPTSVIQLIQKVEYAWGIFIRKVLSLIDALYVTPGPLSIFRRDVFKNLGPY
                     RHAHNTEDLELALRMQANNYKIVNAHRAYVYTVAPKTLKKLYKQRLRWTYGFLKNIIE
                     YKRLFFNRKHGYLGMLVLPMATLTIFSALYFTTATVIATAQSFAKRIVQIQTVGLHFH
                     WSWDLQWFYINTEVISFIGLSLFIITTALILLSRKVADGKIGWGRDLVYFLVLYSFIA
                     PLWLAKSVYNLAFSRSTKWR"
     gene            8773..9489
                     /gene="pheS"
                     /locus_tag="VHF05_00790"
                     /pseudo
     CDS             8773..9489
                     /gene="pheS"
                     /locus_tag="VHF05_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
BASE COUNT         2430 a         2370 c         2616 g         2217 t
ORIGIN      
        1 gcttctgcgc aagccgcagc ggcttcgcga atcgcatccc aaattccaaa ccttaataac
       61 gtgtcttgaa ttgcatccga cctagatcct tggagcattc tcttctggtc ttgagatttt
      121 tcaaataaat atgctagggt aggggaagaa gtgcacttcg gcgggttctc cttgttgggc
      181 ggcttgcggg ggagaacctg ccccgaaatt ctatatgatt ataaataaac ggccatatca
      241 ggccgttttt tcgttatttt agttcgattc cagctatatt tgcaaaaaag cacttctttt
      301 gatatagttt ttccgcactg tttcaagctg tcggttgcct tcacggctca agtttccaag
      361 ctaacagctg acagaatttg cgaaacaaat cccttgatta actaatagaa tcttcgaagc
      421 cggatgcgat tcgaggcgcg actgagcatt gcgaccaagc tgtatgagta atactgcgcg
      481 ggagcgggcg gaaggagcaa caaagaagcg cgccggatgc ggagattata tacaatgaaa
      541 gctgaaattc atcctgaata ctaccccgac gcgaaggtct attgcgcctg cggcgccacg
      601 tacacgatcg gctccaccaa gaaggagatc cacgtggaaa tttgcagcgc gtgccatccg
      661 ttctacaccg gcgagtccaa gaccatcgac gccgcgggac gcgtcgagcg cttcaaggcc
      721 cgcaaggccg cagccgcagc cgctcctaag aaggagaaga aggccaaggt aaggaagtcg
      781 gaagaggagt agtaaacaaa aagccgcgca tcatatttct ctgaaaagag gtattatgaa
      841 ggtgcggctt tttgtataag ttaaaatgca aaattcaaaa ggcaaaatta caagtaaaaa
      901 tttaaaagtt tccgaaatta caagattttc atttttaaat tgcgattttg atttttgaat
      961 tttgactttt gaatttcata tggaagacca agaaaaatac aaatcaaacc caaagacaac
     1021 gtatttggcg cagatgtggc agaaactcca tgacgatgaa atggaggttt tgaaaatgtc
     1081 cgaaaacgat ccttcgatga gggatcttgc cgccgatgag ctgaaaaatc tccaggaaca
     1141 gcagagcgcg cttctccggc aaatggacga cattttgaag agcgaagccg aagaggagga
     1201 atttcccaac gagatcgtgc ttgaggttcg cgccggagcg ggcggggagg aggcggcgct
     1261 cttcgccgaa gagctcgcaa acatgtatca gaaatacgcg gcttccaaag gctggggctg
     1321 gaaagtcgtc gacgagtcgc cctcggcgct cggcggctac aaggaggcat cattcgaggt
     1381 gcgcggcaag gacgtctaca agattctccg cttcgagacc ggcgttcacc gcgttcagcg
     1441 cgtgcccgcg accgagaagc agggcagggt gcacacttca accgcgtcag tcgccattct
     1501 tcccatccgc aagaaagtga aatttatcct gaatcccgcc gagctcgaat tcgaaacgtc
     1561 tcgcggcggc ggcaagggag gccagaacgt gaacaaggtt gaaacggcgg tgcgcgtcat
     1621 ccacaagccg accggcctct gggtccgcgc caccgccgag cgaagccagg gcgccaacag
     1681 ggaaatggcg ctcgccatcc tttccgcgaa gctcgaggca ttgaaggaag aggaggaggc
     1741 gaagaagttc gcctcggagc gaaaggggca gatcggcacc ggcgaccgct ccgagaagat
     1801 ccgtacgtac aatttcccgc agaaccgcgt taccgaccat cgcatcaagg agtcatggca
     1861 taacatcgag cagatcatgc tgggacagat cgacgatatt gtcgaggcgc ttcagaacgc
     1921 cacttccttc tccgaaacag acgatgatga ggaataatat ggagcgggaa gcggaatggc
     1981 ttctgcgcga caagtacgac ggcgtcgaga ctcccgaata tcataaagat cttgagcggc
     2041 tcgcggcggg cgagccattg gcgtatacaa taggctggac gccgtttttg aatacgaaaa
     2101 tttttctcga ttccaggccg ctcatcccaa ggcccgaaac cgaatattgg gtcggaaacg
     2161 cgattgccga agccgaaaag aattccaagc cgctcaaaat tctcgacctt tgcgccgggt
     2221 cgggctgcat tggcgtcgct gttctcaagg cgttgccgaa ttccactgtc gattttatcg
     2281 agatcgatcc ggcgcatcac ccgcttatcg cgaaaaatat ccgtgagaat agcatcgatg
     2341 agtctcgcac aagaattttg ggcggcgatc tttttgaaaa cgcaggtacc ggctacgatc
     2401 tcatcctttc caatccgccg tatatcgatc ccgcgctcca gcgtaccgat ggaagcgtct
     2461 tgaatcacga gccgcatctc gcgctcttcg gcggcgaaag aggaatggaa atcattgagc
     2521 gaatcatcgc ggacggccca gcgcatcttg ctctcggcgg cacactctac atcgaacatg
     2581 aacccgaaca ggctgaagcg attgcgaagc tcctgcctgg ctctgttcag cttaaggatc
     2641 aattcggttt gattcgcgta acgcgttata aaaagtaatt agtagatatc catacgtgaa
     2701 ggtttttgat ctgtgagatc ccgcaatact ttacttaatc ggagtaaata taatcaaaaa
     2761 atgaacgcaa aaaaattatt tttaataatc tttatcgtct tatttttaat tctttccgcg
     2821 ggtttcgttt tttggaacaa gcagacatct aaaccagcaa taaatatctc aggttcccag
     2881 caaaattcag aaaatgtcat aggagaagaa tcaaccacta ctaccagctt cttagggtac
     2941 tatctcacaa ataattctgt agctggaaag ccgaaagaat gttccatatt cgttattccc
     3001 aagagcagtg atcctctatt tgattttttc tctgaaatgg tacaacaagg aaatacagta
     3061 aattcaatcg actctaaagg cgatctgttt cttaatgttg atcttgcgaa tgtttccaca
     3121 gttgataagg caagagtcgt atcgtcaaca gaaagcaatc ctattatatt ggacgttcaa
     3181 aagaaaagaa tggaagggaa agatgctgga ccgtgtactt cctttgtgga cattctttct
     3241 gtcgaagtgc caagctaatc tggaagctaa attcgggcaa tatgccaggt tgcaaagttc
     3301 taaaatacct gttactatag cccggtaatt gcttggcgga tttaccgtgc cggaggccgt
     3361 tttttgcaag ggccgaaggt gtaaggatcc gaaattccag gcttttattt aataaccaga
     3421 gcgataattt cgctcgaaaa cttgtaacgg gaattactat gtcagaagca actaccacca
     3481 accccaaggt tgaagagctc ttcaaggcgg gagctcactt cggattcgtg cgctcaaggc
     3541 gccatccgtc ggcaaagcct tttatctacg gcaccaagaa ccgcatcgag attttcgatc
     3601 tcgagaagac ggcggaggag ctcgataagg cggcatcata tatcaaggaa atcgcaaagg
     3661 ggaatggcac tgttctcttc gtcggcggca agagcgaggc gcgcgaggct atcaagagcg
     3721 ccgcggagtc tctcgatatg ccgtacgtcg ccggtcgctg gatcggcggc acccttacca
     3781 acttctccga aatcaagaag cgcgtcgcga agctcgagga tctcacgaac cagaagcaga
     3841 aaggcgagct cgccaagtac accaagaagg agcgcctgct catcgatcgc gagatcgaga
     3901 acctccacag gttcttctcc ggcctcgcca atctcaagga caagcccgcg gtgatgttcg
     3961 tcatcgatgc caagcacgag catatcgccg tggcggaagc gaagaccatg ggcgtgcccg
     4021 tcgtggcgct ctgcggttcg gattgcaatt tcggcgaagt cgactacgca attccgggca
     4081 acgactcgtc ggttccgagc gtcaaatatt tcgtcgagcg catcgccgcc gcctacaagg
     4141 aaggcaaatc ggaaaaagcg ggagcataat cagcgaattt tcaatttcta atttccaatt
     4201 ttcattgaat gctcaatgaa tcaataccgt tgtggaaacg cttgaaaatt gtaaaattgt
     4261 aaattgattg aaaatttaaa attgataatt gaaaatttaa aactatggct actatcacta
     4321 cggaaatgat caaagagctc cgtgataaaa ctggcggcgt ttcggtcatg cagtgcaaga
     4381 aggcgcttga agaggcggga ggcgacatgg agaaggcgct cgtcatcatc cagcgcgctt
     4441 cgaaatcggc ggcgctcaag aaggccgacc gcacccttgg cgccggcgtc gtccagtcgt
     4501 acatccattc gaccggcaat atcggctcgc tcgtgcttct ttcctgcgag acggacttcg
     4561 tgtcccgcaa cgaagagttc aagacccttg cccgcgatat cgcgatgcat gtcgccgcgg
     4621 ccgatcccca gtatttgaag accgaggaca ttcccgaggc cgacaggaag aaggcggaag
     4681 aagtccttgc ggaggaaatc ggcgacaagc ccgaggacat gaaggcgaag attctcgaag
     4741 gcaagctcgc atcatacttc caggagaagg tgctcctcga gcagccgttc atcaaagatc
     4801 cggagcgcac catcggcaat ctcgtcgaat cggcgatcca aaagttcgga gaaaaggtgg
     4861 aactcacgcg atttgtcaga tttacggtgt aattacgcgg taattttacc tgttgaaaag
     4921 accccttcgg gggtcttttt catgatatac ttatgaagta ccggaacacg gtagaattta
     4981 gcatgtaaga aacggatacg gcgcgtcccc cacgttttcg gcgcccgcat ccccaatgta
     5041 tgcaaacaat attcattgtc tccgcgtcga ttatcttcgc gatgatcgcg ctccgagccc
     5101 ttgagcttaa atttcagcgg cctatcattc tcgctcacct gagctggccg gcggataagc
     5161 gcttgagggt ctattacaag aagtttcgtc attatctcga cgaacgtatc aagacgagga
     5221 tcgatgcctt cgccgagcgg cttaaggaaa gggcgaacgc ctatctcgag caggcgcgcc
     5281 ttggcggcgc ggaatacgcc ctgagccttc aggaatcgct tcgcggcaag aaagccctct
     5341 cccgcggttc ggtctcgttc ttccttctca acatttccga ctacaatatt gagaagaaga
     5401 agggtcctga tccggctctc attgccgaat cgatgcctct cgaaggaacg gaagcactcc
     5461 aatagcagat gcaaaatatc gcggcgaaag ccccgatatt ttcgtttgtc atttcgataa
     5521 tgagcttgta tactcatttt gccagccagc atagctcagt tggtagagca atagttttgt
     5581 aaactatagg ccgtgggttc gaatccctct gctggctcaa tttgaaagat ttttaatgcg
     5641 tggcggttaa aggtttagcc gcgtgttcat caaaaggaga tttgagcgcc gtttgaaacg
     5701 ggctggggat gccgcggata tcgtctcgat ggagcgcatg gagccacctt tctttcggct
     5761 ggatgcgggg cgtatggtcg cgcaccggag cggcggcctt cttcgcgaag agggagggga
     5821 gggaatcgaa ggccgtttct cccaacgagt cgagcgtgac gcttcggtcg tagaagtcgc
     5881 gtgctcggag cgcatgccat tcatactggg ctccgtcgcc aacgttaaaa aatttccatg
     5941 cctgccgccc cagcctggaa acatattcct tgagacgaat ggagagcggg atgtgggcgg
     6001 cgggcggagt catgcagaaa ctttagcaaa cacatgctga agttttgatt aattccggcc
     6061 cgcgatcgga atcatggaat ttttcttgac accaagcacg gcagtgtagt accctgcgga
     6121 caggagaaaa gctcatgata gaacacgcga gaaagactgc agcaatccgc tcagcccact
     6181 tcagctcaga gcacgcaaac ggaagatcgt atattccatt gcggctcatt gcgtttattc
     6241 tcgccgcgct gctctggttc gcttgcatcg tttccttttt caggtatgcc gctcccatgc
     6301 tgcatatccc actcgttcat tcattcctta tcgacctggt atttgccgca atcctttcgc
     6361 tcgcggcagc tgccgtctct tgcttatatg tgcgccgttc aatgcgctca ttcgaagaga
     6421 ttccgagcta aaacagatcg accgcacaaa accgcccgcg aagcttacgc ttcgcgggcg
     6481 aattttatcg taatctttct aatcttttat ttcttcgttg tcgaggaagt cgttgcggtg
     6541 gtcgaggttg cttccgtcgt gcttgcgcgg agcgccttga tctcgggaat gatgcgctcg
     6601 atgtcgtcgg tcgactggaa gccgtagtag accttgccgt tcaaaatcat cgcggggagc
     6661 gtcggctcca cgccgtagag cgagatgagc gtgcggaggg cggagagatt gaggttgtaa
     6721 tcgaaggagt agatgcggag ttcggggtaa tcctggcgca tctgggtgag cacgtagccg
     6781 gtcttctcgc attcggggca gtcgccgttg ttcgagtaga agtaaaggat gaagatcggc
     6841 ttcacgtcgc acttgtcggc gatcttctgc atgaggatgt aatccttgat ctcgaggatc
     6901 gagtagtact ccttgagcgt gatcacttcc ggatcgtcgg gactgcggtt cgattccatg
     6961 tacgagagct gttcggcgat ggagttgagt tccttggaaa gggcggaatt gccgagatcc
     7021 tcgcatgata cctggccgag aaggtcgaac tgcgtttcgg acgactggag gtcgatcgag
     7081 atctgatcct ggatcgagcg catctcggaa atcctcttct ggttgaggta gttcgaggcg
     7141 aagaacgcca ggacgaatat gactgccgtg atcgcgaaag cgaaccagta tttttttgct
     7201 ccgaatttat ccatcctgta gtgaaatttc aggtcgttcg cgcgtacgcg aactgtcctt
     7261 gatatcttta cgcttgttta gattgaagta taaggtacca tagcagattt ttacaagtaa
     7321 gcgcaacctg aaattctact acaggatcca tgattcttat agatctaccg ccacttggtc
     7381 gagcgcgaga acgcgaggtt atacaccgac tttgcaagcc acagcggagc gataaacgaa
     7441 tacagcacga ggaagtagac gagatcgcgt ccccagccga tcttcccgtc cgccaccttc
     7501 cgcgagagga ggatgagggc agtcgtgatg atgaagaggg aaaggccgat gaacgagatc
     7561 acctccgtat tgatatagaa ccactggagg tcccagctcc aatgaaaatg gaggccgacc
     7621 gtctgtatct gcacgatgcg cttggcgaac gattgcgccg tagcgatgac ggtcgcggtg
     7681 gtgaagtaga gggcggagaa gatggtcaag gtcgccatcg gcaggacgag catgccgaga
     7741 tagccatgct tgcggttgaa gaaaaggcgc ttgtactcga tgatgttctt gaggaatccg
     7801 tatgtccagc ggaggcgctg cttatagagc ttcttcaggg tcttcggcgc cacggtgtag
     7861 acgtaggcgc ggtgggcgtt cacgatcttg tagttgttcg cctgcatgcg gagcgccagt
     7921 tcgaggtctt cggtattatg ggcatggcgg tagggaccga gatttttgaa gacgtcgcgg
     7981 cggaagatcg agagcggccc cggcgtcaca tagagagcat cgatgagcga aagcaccttg
     8041 cggatgaaaa tgccccatgc gtactcgacc ttctggatga gctggatgac ggacgtcgga
     8101 ttgtgcacct tgatcgacgg cacgactgac atcactttcg gatcatcgaa aaacggcatg
     8161 atctcgttca aggcctgcgg atcgacgaac gagtcggcat caaggcagcc aacgaattcg
     8221 ctgtcgcttc gctcgaggcc gtaattcaag gccgtgtatt tgccgccgtt cggcttcttg
     8281 gcgatcttta cctggggatt cccggtgaag ctctggagca cctcccaggt gccgtcggtc
     8341 gagccgtcgt cgaccacgag gacggaaagc ttgtccttcg gatagtcgag cgcgagaaga
     8401 gaattgaccg taccggaaac agtcttttcc tcgttccaga ccggcaccat gatagtgact
     8461 gtcgggtggc tggtaagcgt tctcatgccg cccgcctcat caacttcctc ctgccgctca
     8521 aaatacgtta taagcaaaaa aacctcgaaa tagagggata aaaagaggaa aatatagacg
     8581 attgcagtgg taaaggtggc catgtggggg atgggcgaat caggaacaat cttaccattt
     8641 ttgaagctct tcttcaatag cggagccgtt ttttgggaag gctactcgag ccttgccgag
     8701 agagcgcctg cgttgccgtt tggggcccga gtccttataa tggggcctat gaatgaatct
     8761 ccggaacgga agggccatct ccatccgatc acccaggtaa cgaggcggat cgcctcgatt
     8821 ttcgacgaac tcggctttga ggtggcagag ggtcctgaga tcgaggagga attctataac
     8881 ttcgacgctc tcaatatccc cggcgaccat ccggcccgcg acatgtggga tactttttgg
     8941 ctcaaaccct tgaacgcccg caagctcctc cggacccata cctctcccgt gcaggtgcgc
     9001 tatatggaga aaaaccggcc gccgatcaga atcatcgcgc ccggcaagac cttccgccag
     9061 gaagcgaccg acgcgacgca cgaggcgcag ttctatcaga tcgaaggatt gatgatcgat
     9121 gagaagacga gcctcgcgga tttgaagggc gtgctcgaaa actttttcga gaagcttttt
     9181 ggaacgaaaa ctaacgtgag attgcgcgcg aattactttc cattcgtcga gcccggcgtt
     9241 gaagtggata tgtcgtgctt taagtgcgga ggagcgggat gcaacgtttg caagcacaaa
     9301 ggatggatcg agatcatggg agcgggaatg gtgcatccga aggttttgca ggccgtcggc
     9361 attgatccca gaaaatatca ggggttcgcg ttcggcggcg gtatcgaccg actcgccatg
     9421 cttaaattcg gcattgatga cgtgaggctt ttctacaatg gcgatttgcg cgtcataaat
     9481 cagttctaag atgaaagttt cctataattg gctcaagaca tatttcgata tcgaattgcc
     9541 cgaacctcaa aaaatcgcgg acgttttcaa tatgaacgtc tttgagatcg acgatctgga
     9601 aaatttgccg aatggagatg cgataatcga tat
//
LOCUS       DASZYS010000015         8730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_918595, whole genome shotgun sequence.
ACCESSION   DASZYS010000015 DASZYS010000000
VERSION     DASZYS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918595"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..269)
                     /locus_tag="VHF05_00795"
     CDS             complement(63..269)
                     /locus_tag="VHF05_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79495.1"
                     /translation="MDEEISKKIDVLESRIIEMNATVDKLRKYFLWTLIITVALFVLP
                     LLAMGFVIPFFMNTYVSSLQGLGV"
     gene            524..2230
                     /locus_tag="VHF05_00800"
     CDS             524..2230
                     /locus_tag="VHF05_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVS79496.1"
                     /translation="MEETITASAVPEASGGAPSGETSAFRKWGPLAVLSLALAIIIID
                     TTVLNVSLSTIIEEFGTTIQSLQWVITLYSLILTAFTITGGRLGDLFGRRRMFVVGAV
                     IFAVGSFITSISHGVGVMILGEAVVEGLGAALMMPATASLLLTNYKGRDRSTAFGVWG
                     GVAGASSAIGPILGGYFTSNFSWRWAFRINVFVVIAVVIGSFLIKEARDTEHRPSLDW
                     IGIILSSLGLLLIVFGVIESSTYGWWIASAPFALGSRTFTFFHNLSITVLAIDWGVVF
                     LALFILWELRVTAKGKLPLVSMKLFENKQFTSGAVTMMVIFLGQSGMFFTMPIFFQAV
                     RGLDALHTGLSLLPLSLAVLVAAPLSGGVFARRFTPKRIAQAGILIDIVAFLVLFWTI
                     SISSSVWTLLPGLSLFGIGMGLLMAQLSNITLSAVRTEEAGEASGVNNTFRQLGGTLG
                     TAIIGAILLSTLASGVSNGVAKSAVIPADMKPQISAALAEQSGSIEFGGASGLEGRLP
                     ANVSAEVSTIVKQATVDGNKDAFLYAALFAALSFLVSFLLPNKKNIEHPEEAAAAAPA
                     DH"
     gene            complement(2657..3295)
                     /locus_tag="VHF05_00805"
     CDS             complement(2657..3295)
                     /locus_tag="VHF05_00805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79497.1"
                     /translation="MHHSSTEFNDLISDSSAFAELMEYNCSPGSSVREWEDKRKFIAQ
                     VIPGNGTILDIGCAGGFFLRSLQEWTGFDLIPYGIDINEDFIRKAQELFPACADHFLR
                     MDVRNIAEFPSFGFPDHYDFTYWNFPGNWDVRDAEWTSLIETTLLPMTKKRIIIGFYG
                     SNAFPFDSKEWNDERERIKRRVENLRETGLAVSGTMFNPTQFNQAIAWIDRN"
     gene            complement(3305..3934)
                     /locus_tag="VHF05_00810"
     CDS             complement(3305..3934)
                     /locus_tag="VHF05_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVS79498.1"
                     /translation="MFDIPSLIAAAGVLGVAAVIFAETGLLIGFLLPGDSLLFPAGFL
                     ASQGHLNIWALIGATTVAAILGDSLAYWIGKKTGPALFDREDSLLFKKHYVKKTHDYF
                     EKYGAKTVVIARFIPIVRTFAPVVAGIGEMRYRTFLIYNVVGGALWAAGLSLLGFFLG
                     AWIPDIDHYLLPIVALIIVLSALPPIIHILKEKENREALKRAWQRLTKK"
     gene            4008..4478
                     /locus_tag="VHF05_00815"
     CDS             4008..4478
                     /locus_tag="VHF05_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HVS79499.1"
                     /translation="MEKRPHNNFVETIAVKLTSWVGSTSSIIFHSIVFVAFFILGLLG
                     VSWDMLLLVLTTLVSLEAIYLSIFIQMTVNRHTASLQEVETDIDEIREDVEEITEDVG
                     DIQEDVEELSEDVEEITEDDRKDDARDEVHALVLEDIQRTLRKLMDDIERLKNG"
     gene            4592..6151
                     /locus_tag="VHF05_00820"
     CDS             4592..6151
                     /locus_tag="VHF05_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVS79500.1"
                     /translation="MMERGKNFLRRFKRPKYWITALILALIVIGIVHRAVSSKAPAFD
                     TATVTRQTVAQEVDATGNVKSAEAVDLAFETGGKVANLAVKVGDRVKAGDRLANLSNA
                     DAAANLASAQAKLDQLLQGARPEDLAVSQSSVDKANSDLENAELDVQNKMSDAFVKAD
                     DAVSNHADKLYENAKTSPSFGATVSSGGTTYRISAPDTNTAVKLSQERRDAEDILEGW
                     SSLSASGDTDAAATKAEDGLSKIQTMLSDIADAINSLHTDDSATQEIYTSFKTEVSTA
                     RTDVSAALASLRSSLAAYDDAKSALAIAQKQQDLKAAPATSEDIKIQQAAVDVARAAY
                     NKTVITAPFDGLITKVEPTAGEIVGANTPVISLIADDKLEVEVDIPEADIPKVHLGAE
                     ADVTFDAYGKDFVATSTVAEIDPAATVVEGVPTYRTVLLFSTIDPRIKSGMTANLTIH
                     GDSRENVLAVPQRAVIIRDGQNIVEKVIDGKTVDTPVETGLRGSEGTIEILSGLNEGD
                     QVVASPSATGN"
     gene            6154..6873
                     /locus_tag="VHF05_00825"
     CDS             6154..6873
                     /locus_tag="VHF05_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVS79501.1"
                     /translation="MALIDIRNLKKAYHDEGAETQVLHGVSFTIDAGEFVSIMGPSGS
                     GKSTLLHILGFLDDFDSGEYIFDGRPVSGYTPDEVAELRNSQLGFVFQAFNLLPRTSV
                     LENVKLPLLYSDVPESKWDNMARTAIEAVGLSHRLDHDSSQLSGGERQRVAIARALVL
                     KPAVIFADEPTGNLDSTSGKAVMEIIQRLNEEEKHTVVLITHETKTAEHAHRIIHIAD
                     GRIESEEEVKHRRRATDAFIK"
     gene            6982..8220
                     /locus_tag="VHF05_00830"
     CDS             6982..8220
                     /locus_tag="VHF05_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVS79502.1"
                     /translation="MRLSKQLQTAYGGLRTNRSRSLLTILGIVIGITAIMLVVSLGAG
                     AQNLILSEVQGLGTQTIAVLPGREPTSPTDAATSILSDSLKEKDLIALRNKGNVPGVK
                     EVEPIVIGAGVASYGSNVYQTTIFGTSQLAEGLLNLKESEGVFYDEADVQAHANVAVI
                     GTKVADKLFDGDDPLNQTIKIKNRSLRVIGVLKGTGGSFINVDEMILTPYTTAQEYLL
                     GINYFSRLIVEADDGADVNTVANDVKLTLRESHNISDPSKDDFNVQTQQELADTLSTI
                     TNALTYFLVAVAAIALFVGGIGIMNIMLVSVSERTREIGLRKALGATEGDILSQFLLE
                     SVLLTAVGGVIGIILGTSLAFLISIGLSKGLGVDWKFVFPWMGAALGFGVSALIGLIF
                     GGYPARKAAQKSPIEALRYE"
     gene            8420..8683
                     /locus_tag="VHF05_00835"
     CDS             8420..8683
                     /locus_tag="VHF05_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HVS79503.1"
                     /translation="MAKAEKSNSAFMKPLNLSPELEAVVGKGPMPRSEVTKKIWEYIK
                     KNGLQDENNKRNINADETLKKVFGGKGMVNMFEMTKLVSQHLS"
BASE COUNT         1925 a         2566 c         2372 g         1867 t
ORIGIN      
        1 ttaaaaaccg ccccgtgaag aagcgctgag cgcttcttca cggggcggtt ttcatattaa
       61 atttaaacgc ccaaaccttg aagacttgat acgtacgtat tcatgaagaa ggggatgacg
      121 aagcccatgg cgagaagcgg aagcacgaaa agggctacgg tgatgatgag cgtccaaaga
      181 aaatacttcc gaagcttgtc gaccgtcgcg ttcatttcga tgattctcga ctcgaggaca
      241 tcgattttct ttgagatttc ttcatccatc ctgtagtgaa atttcaggtc gttcgcaatg
      301 cgaacaattc ccgagttatg ccaataattt cattaaaaat ttttccgcat atcctatagt
      361 tcactggcta cctttaaaat ctgaaattct actacaggac catgtccgta gtatatcacg
      421 agggtccgcg ccccttccgt cctggacaaa gggaagcgga cacgctatcc ttattcggaa
      481 ttcgggcaaa ctatttcggc aatctaacta aattaaccaa tccatggaag agacaatcac
      541 cgcatccgcc gttccggaag cttccggggg agcgccgtcg ggagaaacta gcgcattccg
      601 aaaatggggc ccgctcgccg tcctttccct cgccctcgcg atcattatca tcgacacgac
      661 ggtcctgaat gtctcgctct cgaccatcat cgaggagttc ggcacgacga tccagagcct
      721 ccagtgggtg atcacccttt attcgctcat tctcaccgcc tttaccatta ccggcggccg
      781 cctgggcgac cttttcggcc gcaggcgcat gttcgtcgta ggcgcggtga ttttcgccgt
      841 cggctccttc attacctcga taagccatgg cgtcggcgtg atgattctcg gcgaagcggt
      901 cgtggaaggc ctgggcgcgg cgctcatgat gcccgccacc gcttcgcttc tcctcaccaa
      961 ctacaagggc cgcgaccgca gcaccgcctt cggcgtgtgg ggcggcgtcg ccggagcctc
     1021 gtccgcgatc ggcccaatcc tcggcggcta tttcacctcg aacttctcgt ggcgctgggc
     1081 gttccgcatc aatgtctttg tcgttatcgc ggttgtcatc gggtcgtttc tcatcaagga
     1141 ggcgcgcgat accgagcaca ggccgagcct cgattggatt ggaatcattc tctcgtcgct
     1201 cgggctcttg ctcatcgtgt tcggcgttat cgaatcctcg acctacggct ggtggatcgc
     1261 gtcggcgccc ttcgccttgg gctcccgcac gttcacattc ttccacaatc tctcaattac
     1321 cgtgctcgcc atcgactggg gcgtcgtctt tctcgcgctc tttatcctgt gggagcttcg
     1381 cgttacggcg aagggcaagc ttcctctcgt ttccatgaag cttttcgaaa acaaacagtt
     1441 cacttccggc gcggtgacga tgatggtgat cttcctcggg cagtcgggca tgttctttac
     1501 gatgccgatc ttcttccagg cggtgcgcgg cttggacgcc ctccataccg gcctctcgct
     1561 tctgccgctc tcgctcgcgg ttcttgtcgc ggcgccgctt tccggcgggg tcttcgcgcg
     1621 ccgcttcacg ccgaagcgta tcgcccaggc gggaatcctc atcgatatcg tcgcgttcct
     1681 cgtgctcttt tggacgatct cgatctcatc atccgtttgg acgcttcttc ccggcctctc
     1741 gctcttcggc attggcatgg ggcttctcat ggcccagctt tccaatatca cgctctccgc
     1801 ggttcgcacc gaggaagcgg gggaggcgtc gggcgtcaat aacaccttcc gccagctcgg
     1861 cggcacgctc ggcaccgcga tcatcggcgc gattctcctc tcgaccctcg cttcgggtgt
     1921 ttcgaacggc gtcgcgaaaa gcgctgttat tcccgccgat atgaagccgc agatctccgc
     1981 ggcgcttgcg gagcagtcgg gctcgattga attcggcgga gcctccggcc tggaagggcg
     2041 gcttcccgcc aatgtgagcg ccgaagtctc gacgatcgtg aagcaggcga cggtcgacgg
     2101 caacaaggat gcgttcctct atgccgcgct cttcgccgcc ctctctttcc tcgtatcatt
     2161 cctcctgccg aacaagaaga atatcgagca tcctgaggaa gccgcagccg cagctcccgc
     2221 ggatcactaa agaaaatcaa aaattaaaga aaaaaccgct cgaacggctt gcgtcgttcg
     2281 ggcggtttcg aatttttcag cacagctcta ttggcagctg cactgggcca actatttcct
     2341 taaagtgctg ttccgcattt tccaggcggt caatatatcg tttgtgcgat gaagggggca
     2401 ggacaatgcg cacggtgtcg aggttggtcc gggccaaacc taagcattgt tccagccgag
     2461 cggttaattt tcgcgtggcc tccgcgttat tccgagcttt tctaagcctg tcactaagaa
     2521 ttgattccgc ctctttcaga gcgaggcaga aagactcctc tttgtgcatt gaagaactcc
     2581 tttacgtgga atattatgtt attattatta acaaaagtca acaatacttt ccaaaaatat
     2641 acactagtgg agtaatttag tttctgtcta tccacgcaat tgcctggttg aactgagtag
     2701 gattaaacat tgtgccggaa acggcaagcc cggtttctcg caggttttcc acccgccgtt
     2761 tgatgcgttc acgttcgtcg ttccactctt tggaatcaaa aggaaacgca ttcgatccgt
     2821 aaaaaccgat gataattctc tttttcgtca tcggaaggag agttgtttca atgagcgaag
     2881 tccattccgc atctcgtacg tcccagtttc cgggaaagtt ccagtacgta aaatcgtaat
     2941 gatcagggaa gccgaacgaa gggaactctg caatattccg cacgtccatt cttaaaaagt
     3001 gatccgcgca tgcggggaat agctcctgcg cttttcggat aaaatcctca ttgatatcga
     3061 taccgtaagg gatcagatca aaacccgtcc actcctgaag cgagcggagg aaaaatccgc
     3121 ctgcgcatcc gatatcgagt atggttccat ttcctggaat gacctgtgcg ataaattttc
     3181 ttttgtcttc ccattcccgt acgctgcttc caggcgaaca gttatattcc atgagctcgg
     3241 caaatgcgct tgagtcggaa atgagatcat tgaattctgt cgatgaatgg tgcatgggat
     3301 aagactattt tttcgtgagt ctctgccagg cgcgcttgag cgcctcgcgg ttttccttct
     3361 ccttcaatat atggattatg ggcggaagtg ccgagagcac gatgataagg gcgacgatgg
     3421 gcaggagata atggtcgata tcgggaatcc acgcgccgag aaagaagccg agaagggaaa
     3481 gtccagccgc ccagagcgcg ccgcccacga cattatatat gagaaacgtc cggtagcgca
     3541 tttctccgat gccggccacc accggcgcga aggtgcgcac gatgggtatg aagcgcgcga
     3601 tgacgaccgt cttcgcgccg tacttctcga agtagtcgtg cgtctttttc acgtagtgct
     3661 tcttgaagag gagggaatcc tcgcggtcga aaagggccgg gccggtcttc ttgccgatcc
     3721 aatatgccag gctgtcgccg aggatcgccg cgacggtcgt cgcgccgatg agcgcccaga
     3781 tattgagatg cccttgggat gcgagaaagc ccgccggaaa gagaaggctg tcgccgggaa
     3841 gaaggaagcc gatgaggagc cccgtttcgg cgaagatgac ggcggcgacg ccgagtactc
     3901 ctgcggccgc gatgagagag gggatgtcga acatgcgagc aatcctatca cagaaaaagg
     3961 aattcggaat gcgctggtgc ggcagtttgc tatactgaag ccagtacatg gaaaagcgtc
     4021 cgcacaacaa cttcgtggaa acgatcgccg taaaactcac gtcctgggtg ggttccacgt
     4081 cgtcgatcat cttccatagc atcgtcttcg tggcgttttt cattctcggc ctgctcggcg
     4141 tctcctggga catgcttctc ctcgtcctca ccacgctcgt gtcgcttgaa gcgatctatc
     4201 tttccatatt catccagatg acggtcaacc gccacaccgc aagcctccag gaagtcgaga
     4261 cggatatcga cgagatccgc gaggacgtgg aggaaatcac cgaagacgtc ggcgacatcc
     4321 aggaagacgt cgaggagctc tctgaggatg tcgaagaaat cacggaagac gaccgcaagg
     4381 acgacgcccg cgacgaggtc cacgccctgg tactcgagga catccagcgc acgctccgga
     4441 agctcatgga cgacatcgaa cggctcaaaa acggctgaaa gccgtacaac cggtttgaca
     4501 ttcgcccatg gcgcactatg attaaaaagt taggaactct aaataaaatc ccggtacaag
     4561 ctcttcaata gtttaaataa acagaaaaac aatgatggaa cgaggcaaaa attttcttag
     4621 gaggttcaag cgtcccaaat actggatcac ggcgcttatc ctggctctca tcgtcatcgg
     4681 catagtccac agggcggtca gttccaaggc tcccgcattc gataccgcga ccgtgacgcg
     4741 ccagaccgtc gcccaggaag tcgacgcgac cggcaacgtg aagtcggccg aagcggtcga
     4801 tctcgcgttc gagacgggcg gcaaagttgc gaatcttgcg gtcaaggtgg gggatcgggt
     4861 aaaggccggc gatcggctcg ccaatctttc gaatgccgac gcggcggcga acctcgcgag
     4921 cgcccaggcg aaactcgacc agcttctcca gggcgcccgc cccgaggacc tggccgtatc
     4981 gcagtcatcc gtcgacaagg cgaattccga tctggagaat gccgaactcg acgttcaaaa
     5041 caagatgagc gacgccttcg tcaaggcgga cgatgccgtc tcgaatcatg ccgacaagct
     5101 ctatgagaac gctaagacga gcccttcgtt cggcgcgacc gtctcgagcg gcggcacgac
     5161 gtacaggatt tcggcgcccg acaccaacac cgccgtgaag ctctcgcagg agcgccgcga
     5221 tgcggaggat attctcgagg gctggtcgtc gctctccgct tcgggggaca cggacgccgc
     5281 cgccacgaag gccgaggacg gcctgtccaa gatccagacc atgctctccg acattgcgga
     5341 cgccatcaac tcgcttcata ccgacgattc ggcaacccag gagatctaca cgtccttcaa
     5401 gaccgaagtt tcgacggcgc gcaccgacgt gagtgcggcg ctcgcgagcc tccgctcgtc
     5461 gctcgccgcc tatgacgatg cgaagtcggc tctcgctatc gcccaaaagc agcaggatct
     5521 caaggctgcg cccgccacgt ccgaggatat caagatccag caggcggcgg tcgacgtcgc
     5581 ccgtgcggcg tacaataaga ccgtgattac cgctccgttc gacggtctca tcaccaaagt
     5641 cgagccgacg gcgggcgaga tcgtgggggc gaacacccct gtcatctcgc tcatcgccga
     5701 cgacaagctc gaggtcgaag tcgatattcc cgaagccgat attccaaaag tccatctcgg
     5761 cgccgaagcc gacgtgacct tcgacgcgta tggcaaggat ttcgtcgcga cgtcgaccgt
     5821 cgcggagatc gatccggcgg cgaccgtggt cgaaggcgtg ccgacctatc gcaccgtcct
     5881 gctcttctcg accatcgacc cgcgcatcaa gtccggcatg acggccaatc tcaccatcca
     5941 cggcgacagc cgcgaaaatg tgctggcggt gcctcagcgc gcggttatca tccgcgacgg
     6001 gcagaacatc gtcgagaagg tgatcgacgg caagaccgtc gacactccgg tcgaaacggg
     6061 cctccgcggc agcgaaggca cgattgaaat cctgagcggc ttgaacgaag gcgaccaggt
     6121 tgtagccagc ccgtcggcga cgggcaacta gcaatggcac tcatcgacat ccgcaatctt
     6181 aagaaggcct accatgacga gggagcagaa acgcaggtgc ttcacggcgt gagcttcaca
     6241 atcgacgccg gcgaattcgt ctccatcatg ggcccgtcgg gatcgggcaa gtcgacgctc
     6301 ctccacatcc tgggctttct cgacgatttc gattcgggcg aatacatctt cgacggccgt
     6361 cctgtctccg gctatacgcc cgacgaagtg gcggagctcc gcaattcgca gctcggcttc
     6421 gtgttccagg cgttcaatct cctgccgcgc acgagcgtgc tcgagaacgt gaagctgccg
     6481 ctcctgtatt ccgacgtgcc cgaatcgaag tgggacaaca tggcccgcac cgcgatcgaa
     6541 gcggtcggcc tctcgcatcg cctcgatcac gattcctccc agctttccgg cggcgagcgc
     6601 cagcgcgtgg cgattgcccg cgcgctcgta ctcaagcccg cggtcatctt cgcggacgag
     6661 ccgaccggca acctcgactc gacctccgga aaggcggtca tggaaatcat ccagcggctg
     6721 aatgaagagg agaaacacac cgtcgtcctc atcacccacg aaacgaagac cgccgagcat
     6781 gcccaccgca tcatccatat cgccgacggc aggatcgaga gcgaggagga ggtcaagcat
     6841 cggcgcaggg cgacggatgc atttattaaa taattttcaa tttacaattc gaaattttca
     6901 atgaattttc aaatctcaat gccttaattt taaaattgaa acattgaaat ttgattgaga
     6961 attggaaatt gaaaattcgc tatgagactt tcaaaacagc ttcaaacggc atatggcggc
     7021 ctccgcacca acaggtcgcg gtcccttctc acgattctcg gcatcgtgat cggcatcacc
     7081 gccatcatgc ttgtcgtctc ccttggcgcc ggcgcccaga acctcatctt gagcgaagtg
     7141 caggggctcg gcacccagac gattgccgtg cttcccggac gcgaaccgac gagcccgacc
     7201 gacgccgcca catccatcct ctccgacagc ctgaaggaaa aggaccttat cgcccttcgc
     7261 aacaagggta atgtgcccgg cgtgaaggaa gtcgagccga tcgttatcgg ggcgggcgtc
     7321 gcctcctacg gctcgaacgt gtaccagacc actatcttcg gcacgtcgca gctcgccgaa
     7381 ggattgctca acctcaagga atcggaagga gtcttttatg atgaagcgga cgtgcaggcc
     7441 catgcgaacg tcgcggtcat cggcaccaag gtcgccgaca agcttttcga cggcgacgat
     7501 cccttgaatc aaaccatcaa gataaagaac aggagcctgc gcgtcatcgg cgtgctgaag
     7561 ggcaccggcg gctccttcat aaatgtcgac gagatgatcc tgaccccgta cacgaccgcg
     7621 caggagtatc tcctcggcat caattatttc agccgcctta tcgtcgaggc ggatgacggc
     7681 gccgatgtca acaccgtggc gaatgatgtg aaattgacgc ttcgcgaatc gcacaacatc
     7741 agcgatccgt cgaaggacga cttcaacgtc cagacccagc aggagcttgc cgatacgctt
     7801 tcgacgatca caaacgcact gacgtatttc ctcgtggcgg tcgcggccat cgccctcttt
     7861 gtcggcggca tcggcatcat gaacatcatg ttggtttccg tgtcggagcg tacgcgcgaa
     7921 atcggccttc ggaaggccct cggcgcgacg gaaggggata tcctctccca gttcctcctc
     7981 gagtcagtcc tccttacggc tgtcggcgga gtgatcggca tcattctcgg cacgtcgctc
     8041 gcattcctca tatcgatcgg cctttccaag gggctcggcg tcgactggaa attcgtcttt
     8101 ccctggatgg gcgccgcgct cggattcggc gtttcagcct tgataggcct cattttcggc
     8161 ggctaccccg cacgaaaggc tgcccaaaaa agccctattg aagctttgcg atacgaataa
     8221 aattctggct ctattcttta ctttgccggt tattacgcct tcgtacgggg caagtcccgc
     8281 acaagggtcc gacctgcccc gtagcaaacc gcaggttctg ctaccggggt cgaagccctg
     8341 cggtacgaat agttgagcta ggcacgggaa gcgcaatatt gtataattgg cggcattaat
     8401 cgcatacata tcgaaacgca tggcaaaagc agaaaaatcg aactccgcat ttatgaagcc
     8461 ccttaacctg agccccgaac tcgaggccgt ggtggggaag ggtccgatgc ctcgctcgga
     8521 ggtcactaag aaaatttggg agtacatcaa aaagaacggc ctccaggacg aaaacaacaa
     8581 gcgcaacatc aatgccgatg agacgctcaa gaaagtcttc ggcggcaagg ggatggtgaa
     8641 tatgtttgag atgacgaagc tcgtctctca gcacctgtcc taaccaagcc ctcgccttcg
     8701 gcgcattcct ttgtcgcagg ctccgtttgc
//
LOCUS       DASZYS010000016        30771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_398800, whole genome shotgun sequence.
ACCESSION   DASZYS010000016 DASZYS010000000
VERSION     DASZYS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 30771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398800"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHF05_00840"
     CDS             <1..213
                     /locus_tag="VHF05_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HVS79504.1"
                     /translation="RILHTKKEIEELGKAEAQKGLTIVPFSLYNKGRKIKVEVNIVRG
                     RKKYDKRDVLKKRDTEREIRREYSDR"
     gene            325..723
                     /gene="ssrA"
                     /locus_tag="VHF05_00845"
     tmRNA           325..723
                     /gene="ssrA"
                     /locus_tag="VHF05_00845"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            865..1104
                     /locus_tag="VHF05_00850"
     CDS             865..1104
                     /locus_tag="VHF05_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVS79505.1"
                     /translation="MHYCYILKSTKDSSYYIGSTQDLKKRLEKHNRKEVTYSSTRAPF
                     KIAWYAAFQTKDLALAFEKYLKSSSGFAFRNKRLI"
     gene            complement(1169..2050)
                     /locus_tag="VHF05_00855"
     CDS             complement(1169..2050)
                     /locus_tag="VHF05_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HVS79506.1"
                     /translation="MKKRLIGALVLIAYGTVLIKVMVFKAVPAIRVGQLMLNFGGTES
                     GKPANFVPFKTILPYLLGYKGWIIAGINLVGNIALLVPLGFLLSFAFRNITWKKTLAV
                     AVTAGLLIETMQTALRVGIFDIDDVILNALGVMVGFAAYLILAKWVRERKYVHIIVAA
                     IVVIGAAAGAFYAIYPHGEPLIVPDGGDAGQFGGAGNEQEGAIPQSGDPCGGTGGTGE
                     IVDEEANAITIKRQDGVIQTINLTAQTDIRNSAGPIPESDLKIGDHVTVVVLDENDTA
                     STVLVCNAPETETQSGG"
     gene            complement(2120..2776)
                     /locus_tag="VHF05_00860"
     CDS             complement(2120..2776)
                     /locus_tag="VHF05_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79507.1"
                     /translation="MNKKNKIVLSLIILLGVISLGLLAWHGGPGTNGVLLKSEVPQTQ
                     DWKTYQNVAHGYAVSYPEDAAVQPVAEMEGKPVEQSDDIIIEIPGVGEKFGATIYLPF
                     QNAPKALTEERNKLIMLPLFQFAESIRQMQLDDTNPYTKNKKVGGLNEIDLEGQKAYS
                     FTLTKSFSQGLAGGYSLGKEGTVFNFVFVENKSGEKIMIHYPLNDDVAERMIDSFELQ
                     "
     gene            complement(2859..4082)
                     /locus_tag="VHF05_00865"
     CDS             complement(2859..4082)
                     /locus_tag="VHF05_00865"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVS79508.1"
                     /translation="MKAALSVAFLFCLLVVTQADAQTTAYYQLTPFPLKKSDGSAMPQ
                     DLEKIHLWDGWLPSYYYGQTLQRDDKLQMGGWGDVYETYIKFDLNGLPANPSAVTIWF
                     KAYNRGDSSTTTPLAFCKVGGSWNLSLTWSARPSFLTCAGWFDAPIAGGWWGVDFTPW
                     YAEWKNGTVTNNGIALSPEYNNNNFDVLRSSRYTTNDGDRPYLEFTFTQPSGMPSFKM
                     PVPGGAKWLLTNEIGGYECMGESPWPDPYHQGSNYYAIDISPTNVKDGGGSYTGNIPV
                     IAAAGGYVKTVSYTDANGYYVVIDHDGDGNLGTGFSTRYLHLQSNVVVVQNSTVAQGD
                     LLGYMGNTGNPTTGTHLHFGIRYADSGSSSVSQLSYVVMDGWLMKSFQTECAVNSSGV
                     PTSRIRYYHSTNRTY"
     gene            complement(4279..5106)
                     /locus_tag="VHF05_00870"
     CDS             complement(4279..5106)
                     /locus_tag="VHF05_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVS79509.1"
                     /translation="MNYKTFAAGAVALSLAFSAVVPFANAQTASSASSSGAASLNLKG
                     APGTILPIAIHAPCFALSRNLRAGLQGDDVKQLQMMLNSATDTQVATSGAGSPGNETT
                     YFGPATLRAVIKFQNKYKADILAPVGLSQGTGFVGSSTLAHLRLIFPCMPGGDQGNGT
                     TTAPTIVSMSPLFGPIGTKVTLTGSGFASTTRVKFGTGQVTDIDVESSTTLSFRVPEG
                     SGVYCGPGMMCPMIYMLFKPGTYEVSVANMQAMSNSVKFEITGPTATTPKVPAASGQ"
     gene            complement(5933..6703)
                     /locus_tag="VHF05_00875"
     CDS             complement(5933..6703)
                     /locus_tag="VHF05_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVS79510.1"
                     /translation="MKTYTVKQVADMAGVTVRTLHFYDEIGLLNPGAVRPNGYREYGE
                     KELIRLQQIMFFRELDFELDEIRKILDAPDFDVRQALRDQRKLIELRRKRLGGLLKTI
                     DRTIISLANKTSKKKKMEDDKLYNSFSTDEVDQYAEEVKRRWGHTEAYKQSTARVAKM
                     SKGDLAAIKRETEELNQALAKAMDQGTESSEVQALIAKHYAGIRRFYDCPMAMYRNLG
                     KMYVDDPRFTATYDKYRPGLVVFMRDAIAHFCDTQEKK"
     gene            complement(6884..6957)
                     /locus_tag="VHF05_00880"
     tRNA            complement(6884..6957)
                     /locus_tag="VHF05_00880"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6921..6923),aa:Val,seq:cac)
     gene            complement(7339..7412)
                     /locus_tag="VHF05_00885"
     tRNA            complement(7339..7412)
                     /locus_tag="VHF05_00885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7378..7380),aa:Pro,seq:cgg)
     gene            complement(7681..7917)
                     /locus_tag="VHF05_00890"
     CDS             complement(7681..7917)
                     /locus_tag="VHF05_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79511.1"
                     /translation="MATIVHEHEHTEKERSGSMGWIIALIILLAVILLFLFAGAFFAV
                     PNSSGGAYPANQPLPAGNIGGGANGGAGMPTSVQ"
     gene            complement(8045..8593)
                     /locus_tag="VHF05_00895"
     CDS             complement(8045..8593)
                     /locus_tag="VHF05_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVS79512.1"
                     /translation="MSARMAIFILFIIAILAAILGYGSLWYLDFIHGNIFYRDGAATT
                     TAPMQGTYQVADVVDGDTIDVLIGGVKTRVRYIGMDTPETVDPDKPVECYGKEASERN
                     KELVSGKAVTLVSDAEDKDKYGRLLRYVYADGTFVNLELVKEGFATAEPIPPDTAHAK
                     EFADAAALARTDGIGLWSACVK"
     gene            8660..9685
                     /gene="mltG"
                     /locus_tag="VHF05_00900"
     CDS             8660..9685
                     /gene="mltG"
                     /locus_tag="VHF05_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVS79513.1"
                     /translation="MATSKKRGKIHRLSRHGAYLIVGIGCLLAVLCGVLILSKPDIPS
                     EATDGKQAANVIVDPGASPQQIVGNLANRGLIKNPLFFRAAVAIASLFHKVQAGGYYI
                     AQGMSDWDIFRSLANPSLRYVTIAAGMRKQEVADLVGDKLDWDTVEKRELVNANMDLG
                     GDDLEGKYFPGTYLFPDDATPEEVAKQMVGNYEEEMEKIKAKYPKSTINYDTALLIAS
                     IIQREAAGKQDMRLISGIIWNRIFDGMSLSMDATVQYARGKTDDGWWAPITASDIRTI
                     DSPYNTYKNDGLPPTPISNPGPDAIDAAMNPQKTSCLFYIHDTHHVIHCSATYAGQLK
                     NINTYLK"
     gene            9799..10311
                     /gene="infC"
                     /locus_tag="VHF05_00905"
     CDS             9799..10311
                     /gene="infC"
                     /locus_tag="VHF05_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVS79514.1"
                     /translation="MSTQVRTNEQIRAAELRVIGPQGENLGIISLAEALRQAREANLD
                     LIEISPAANPPVAKIGDYGKFLYDANKKAKESKTKAHVIEVKTIQIKVGTGEHDLALK
                     ARKISEWIGEGNRVKIDLFLPGRTKYMEQKFLEERLDRVLKLVSEPYKIADPAKKSPK
                     GLTVIIEKDR"
     gene            10313..10498
                     /locus_tag="VHF05_00910"
     CDS             10313..10498
                     /locus_tag="VHF05_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVS79515.1"
                     /translation="MKTNKSYTKRLKVTKKGKIIARKPGHDHFNAKESRRKQLNKRRG
                     ATLTISNKSRARFLPGN"
     gene            10520..10864
                     /gene="rplT"
                     /locus_tag="VHF05_00915"
     CDS             10520..10864
                     /gene="rplT"
                     /locus_tag="VHF05_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006806727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVS79516.1"
                     /translation="MTRVKKGVNALKTRRNTLKATKGYRFGRSTKERQANEAIVHAGK
                     YAFAHRRDKKNDMRQLWNVRISSLLKAEGTSYSKFIGGLKKANIALDRKVLAILAAEN
                     PETFKKVLAKIK"
     gene            complement(11021..12376)
                     /locus_tag="VHF05_00920"
     CDS             complement(11021..12376)
                     /locus_tag="VHF05_00920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVS79517.1"
                     /translation="MKVTFYSGVGSVTGANFLLENGESRVLVDCGMTQGAHSADQFDI
                     NAKPFGYDPASIDVLFVTHAHMDHVGRIPKLVRDGFKGVIYSHPATKDLSELMLPDAF
                     AIMAEDAKRFGKEPYFSQADIDAAFSMWKTVSYYVPTELAGYTVTLKDAGHVLGSTMF
                     FFEKGGKTVVFTGDLGNSPSPLLRDTDSVDGADYLVMESVYGDRNHENRDSRIDKLER
                     VINETMRRGGALVIPAFSLERTQMILYELNDLFERKKIPSVPVFVDSPLAIKVTEVYK
                     RYENLFNDTAKGVAASGDDIFAFPKLSFTLRREQSEAIEKTANPKIILAGSGMSMGGR
                     IQSHEADHIEDPKSTILLVGYQTPGSLGRRLLDGVKETWISGRKRQVRAQIDMIEGFS
                     GHKDSDHLLEFVDEGKSKLKKVFVVMGEPKSSMFLAQRLRDYVGVDAIFPEAGKGYEL
                     K"
     gene            complement(12796..13398)
                     /locus_tag="VHF05_00925"
     CDS             complement(12796..13398)
                     /locus_tag="VHF05_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVS79518.1"
                     /translation="MHPKDERTLVIIKPDGIQRSLVGEIIGRLERVGLKFVAMKMLVT
                     TPEHVEKHYTLDPNWRTVTGEKTIKGYRDKGLTPPSEDPLAITAAILENLKKYMTSGP
                     VIAMVIEGAHAVKIVRKLVGSTEPLSSDVGSIRGDYVLDSYQMSDGDKRAIRNLIHAS
                     GSVEEANMEIAHWFKPEEVTNYRLVQEEILYDVNLDGILE"
     gene            complement(13460..16243)
                     /locus_tag="VHF05_00930"
     CDS             complement(13460..16243)
                     /locus_tag="VHF05_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HVS79519.1"
                     /translation="MEGPLFKEAPQFKTPQEELDYLRAQVRERERELEAHGEKPQLHE
                     IINERVDEYKKAPETVLHPDLKMTQENLNRIILNLPPEAHDAQIDELVEVLREKGIKN
                     AFALLEKLSNPHLDDDFHRYLIEYIKEGFVVPGLKEKSPLYRSLHMTLYEIMIPEGGK
                     DKEGSFKELISGMEQFYAGMRSIPGEKNRGHNYFTIELAMENIGAEFSFYAGVPVDKK
                     LLFEKHILSVFPHAKLVEKIEDYNIFNPQGESAASFAKSAGNPVFPITLYDQFDHDPL
                     NALLSSFSKIDHVGEGAAIQIVMSPDQDDDVLLRYKHALKRIQKGENYKEAIDISYSM
                     TGDLLKGVKDFFKTSKKKDGTPKENTPIDPDQLQGIQNKVASSIFNANIRLVVSAENR
                     MKAEAILSDMESSFNQFSNTKGNGLKWNRVSKGALKSLLHQFSYRLHSQHQNIPLNIK
                     ELTTLIHFPTSGADAAPALRRAKAGSAPAPIGLSETGILLGVNRHRNVETRILMSEED
                     RLRHFYIIGQTGTGKSTFIKNQIIQDIREGRGACYIDPHGSDIQDILAAVPRERYEDV
                     IYFDPSETARPMALNMLEYDRRFPEQKTFVVNELFSIFQKLYSGVPESMGPMFEQYFR
                     NATMLVIEDPETGSTLVDVSRVLSDKKFRDLKLSRCRNPIVVEFWRGIAEKAGGEGSL
                     ANIVPYITSKFDVFLANDIMRPIIAQEKSSFNFREIMDGKKILLVNLAKGRLGDINAN
                     LIGLILVGKILMAALSRVDSFGTELPNFYLYIDEFQNITTNSISTILSEARKYKLSLT
                     IAHQFIAQLEEGIKNSVFGNVGSMAAFRVGAADAEALVKQFEPVFTANDLMNIDNHNA
                     YLRLLANGQPRRPFNIEVTPPEKFDPSHVAELKQLSYLKYGGDREAIEAEIMKKYAKA
                     PAEPQASLGASRP"
     gene            complement(16512..16661)
                     /locus_tag="VHF05_00935"
     CDS             complement(16512..16661)
                     /locus_tag="VHF05_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79520.1"
                     /translation="MADDQNPQADEKDKQNGQPEKSGDENLSPEQMKAQLEKDLKDGD
                     IEILG"
     gene            complement(16682..21973)
                     /locus_tag="VHF05_00940"
     CDS             complement(16682..21973)
                     /locus_tag="VHF05_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79521.1"
                     /translation="MPLGSKPGENPDYRPENDPRIDVVRQALKEGGGRLDPKTRRKLQ
                     GEHGFGSYTLHKVVKAIEAGQLSDAQKFDIAAVLFQAYYPEDPYNEETIRSWVDQFQD
                     QANEDGVDYLAKTLEIGNDPAIQKRINEIARGEISRETAEGEETPFPKTHERWKHEAE
                     RLRTLIGEAYRDAGIDEYFSDDEDFADKVNKDARALLADLKEGRSSDPETRAKQMAEE
                     EKKKLTEAPAVQPEVTGEEIEAEQGEMDEAEAENAKLKPYRDAIKSALRELLETKYSD
                     LIKEGFSLKDFEEYIAENAEWLKGMIDMGESGYGSMEEGVRTISRNLEWVLQMVASIK
                     RNETASSSEQVVETKPETASEEKNAEEEPFQIKEGDFVQWTSQGVDQFETPRKISGFS
                     EDGKLAFVEGGTTGVPVEQLHLEATREEEEKEQKERELARENEMRAAAVPVKYEGDEY
                     LAEKIANDPIVRFWKEKGLSDEDIRKITIVYHVLAPVAEPEKDKEAFDLASASNYHDI
                     ERTRGKLKPEDRERFDSEVRKIRGVEFGNGMNVDVVFDSEFVGRLTKGDLRKYAEEFT
                     KFLVGVRMGQEKDTYAESVERKLAEEAAKEKSEKKAGEEEPEGKKEEAAEPAEAGAEK
                     EEARKERSDAEKNTEQYFKDTFGFKDGDLDKIEGYKDLTDGQKRLVYENLRQVTLGRI
                     KEEARGEFKEDLENAGVLKLGGNGRFAKFDKALGRVWLGATKHYHIAKLEKKSANDIL
                     TGGLKTHGEILQQLVKGTKELGLDAHIENGQVEIDYVAEKDLTPANRELQPAERELIS
                     KFNEAAREFARTPAEWNYSGASKFQNTKYWLVERNFRNLKKKIFDLRLLENGSNRNEA
                     ELYVNTIDERLRANQLFGSNSDIEEELLNIKDRKVWKKVLKDIVTERGIISAAGFLSR
                     STAMAIGGVTMLPFAAAGVGFVTGRSRTRGAQAERAEDMRRGARASLRKDEFKALAAE
                     LDAEKKKLRDLKAGAKLTGTRDAKNDVEAQQAKVNRLAKKFERKTHMERNFVPAEVLV
                     NKIDDLEREFDSVNSNPKFESEKEKDAERERIMHKLKVRLFYTRQKLNRGLVSFGVGE
                     GSDEIAERLREQLELRQKLSEAGTFLAMFEQNRPGKKSTDLAEKTERATKKIKGVLSW
                     REKRINSAQAWQARRGGMRSAAWAFGLSALGQVAHNLLWEHHGTEGEFNSVKDRLSSM
                     EQEFQKVKTGLSNQLAEEAKSEKALKAAMVAHQHAAAHAPAPAHAAVPPAPSAPAHEV
                     PPPPSAPNAEHAVPPPPSAPAAPKEGFTLHPLDQGIVKGAHKPFWDGTELSIYYQGNA
                     SPEQMTSHFADFDQYASQISHEGMYAQVPDMRHLREEVEFLSKSFGGQRPELQQEILV
                     RVQDLHDKLITSGATGRFDSQTDELLHIINERADGMRKILSHEMGSTVGLEQGAADHA
                     AHEATGRAVPSHELHNDGDISNDVDRMRQGMGRGHHAPPVHHEGGNAPIQPEVHNDGD
                     ISNDSTIGSPQGGLNQTPLENLQPSHDPVLGDLHRILGGKEYEKAAKVFSHAEALDQF
                     AGKGITPENAHVIAEDLGYISRNIHDQPLLAQDEVMKHVATIQREFPRMRMFANPADR
                     TEFLSINASMREIIATHQRTVEKFNDVLQGLKYKNGKHAGYGLEWYRNHIAGKSSHTV
                     GELRRTYIANHEDGTENPFVDWVYEKLDAKDVSPADNDALKMPLDEFIRGRLKPDGFG
                     KSLLKSFQNTARIEAEPLHRYGYPPPPPPTRWPFYRR"
     gene            complement(22259..23026)
                     /gene="map"
                     /locus_tag="VHF05_00945"
     CDS             complement(22259..23026)
                     /gene="map"
                     /locus_tag="VHF05_00945"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVS79522.1"
                     /translation="MITIKTAEDIEKLREGGRRHAEMLRKLAAMTEPGVSTEELNAAA
                     LKMIEDGGDAAAFLNYRPRGVKRPYPAALCVSINDIIVHGIPNVNPRVLQEGDIVTLD
                     LGLTHQGLITDAAVTLGVGKIDDDSRRLIHATREALERGIKAVAPGNHIGDIGAALEI
                     FAQEEGYAITEDLAGHGVGYSVHEDPFVPNTGVAGQGPALKPGMVLAIEPMLTLGGGR
                     VVFEGDEYTVRTKDGSRAAHFEHTVLVTEDGVEVLTR"
     gene            complement(23152..23742)
                     /locus_tag="VHF05_00950"
     CDS             complement(23152..23742)
                     /locus_tag="VHF05_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HVS79523.1"
                     /translation="MRAQTIILFGRSGSGKGTQAELLLKFISDHDPIKKTLYIETGQK
                     VRDFMTHDNYSSKLTADIVENGKLLPEFMPIWIWSNYLIEHFTGEEHLVLDGLSRRPH
                     EAPILDSALRFYNREYPTIIVLDVSREWAAERLKGRARADDNDADIRRRVEWYDTNVV
                     PAIEYFRNNGRYSVIDVNGEQEIERVHSDIVSAIDW"
     gene            23946..24515
                     /locus_tag="VHF05_00955"
     CDS             23946..24515
                     /locus_tag="VHF05_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HVS79524.1"
                     /translation="MGKKTIITIAGDLGSGKSSNADKAASLLGYSRFSSGDFARLVAK
                     RHGMSIEELNEHAEEHPEIDHEIDDEVKAAGEREKLVIDSRTAFHWIPDSFKVYLKLD
                     PKLAAARIYEQMLQGQRVSQHADSVDHMYEQTIRRKESERKRYLALYGFDSTDPSGFD
                     LVIDTATAPLEKVSEKIIEEYKKWLGSNE"
     gene            complement(24607..25878)
                     /gene="secY"
                     /locus_tag="VHF05_00960"
     CDS             complement(24607..25878)
                     /gene="secY"
                     /locus_tag="VHF05_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVS79525.1"
                     /translation="MSKFTEKLKLVFGDEDLRKRIFFVLGCFALFRLLAAIPIPGVNS
                     LMLQSFLQNNQFLGLLNIFSGGGLSTLSIVMMGVGPFITSSIIMQLLTIMSPKLKALY
                     QEEGEAGRRKFAQYSRLLTVPLAIIQAYGFLILLERQGIIPGLTNFQLVVNLFVATAG
                     SILLMWIGELINEFGVGNGVSLLIFAGILARLPASTGQLAFAFDATQLPLYAAFLVAA
                     LVVIFGVVIVTEAERPIPVTYAKRVRGNKVYGGVSTYLPLRLNQAGVIPIIFALSILL
                     FPQMIFNFLTASTNSLLASIAQHGVAILNNSWLYAAIYFVLVFIFTFFYTAITFDPEQ
                     VATNLQKNGAFVPGVRPGLSTSEYLADKLTKLTIFGAIFLGLIAVLPLVMRAVTGITS
                     LAIGGTALLIVVQVIVDLIKRIDAQVSIREY"
     gene            complement(26122..26565)
                     /gene="rplO"
                     /locus_tag="VHF05_00965"
     CDS             complement(26122..26565)
                     /gene="rplO"
                     /locus_tag="VHF05_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVS79526.1"
                     /translation="MQFNNVQRKTQRKRAVQVGRGGTRGKTSGRGTKGQKARAGHKIR
                     PEIRDLIKKLPKLRGRGTNYLKSVAVKPTAVNLSVVEAVFKAGDKVTPSTLAAKGVVP
                     KISGKISPVKILAAGELTKKFTIAGCEVSAGAREKIEKAGGKITP"
     gene            complement(26572..27189)
                     /locus_tag="VHF05_00970"
     CDS             complement(26572..27189)
                     /locus_tag="VHF05_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVS79527.1"
                     /translation="MPNDKEQQNMGTAPAADNRAPRGRGGRPAGGKGDFRKNPRKGPR
                     REARVKPEFDHKIVSIRRVARVVAGGRRFSFSVVLVAGNRKGQVGVGIGKAGDTPLAI
                     EKAMRDAKRNMIRVSLTKSGSIPHEVEAKYSSARVLIKPAPGKGVIAGSSVRNVLELA
                     GIKEVSAKIFSRTKNQLNNARSAVKALSKLAKVKEVAVASAASEQ"
     gene            complement(27195..27524)
                     /gene="rplR"
                     /locus_tag="VHF05_00975"
     CDS             complement(27195..27524)
                     /gene="rplR"
                     /locus_tag="VHF05_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVS79528.1"
                     /translation="MAQRARRQKRIRAKVKGTAERPRISVFKSNTAVVLQLIDDTKGA
                     TLAMATTRGGKGKTLVERSLAAGKDLASKAKAQKIEAVVFDRGGYIYTGAIKAAAEGA
                     REGGLKF"
     gene            complement(27570..28109)
                     /gene="rplF"
                     /locus_tag="VHF05_00980"
     CDS             complement(27570..28109)
                     /gene="rplF"
                     /locus_tag="VHF05_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HVS79529.1"
                     /translation="MSRIGKKPIALPKNTEARWSESDGVFSVKGPHGELSRKFKGDIA
                     ITIGEGEVTLAPKKQSIETNALWGTYASHIQNMVDGVNKPYEKKLVVEGVGFKWDAQG
                     DSLNLSLGFSHPVKMKIPAGVTAKTEKNTLTLTSIDKDILGQFAAEVRALKKPEPYKG
                     KGIAYEGEVIRRKQGKKAA"
     gene            complement(28116..28508)
                     /gene="rpsH"
                     /locus_tag="VHF05_00985"
     CDS             complement(28116..28508)
                     /gene="rpsH"
                     /locus_tag="VHF05_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HVS79530.1"
                     /translation="MVTDPLADMITRIKNAGAAGNPLVTVPYSKMKMSIAELLQREGF
                     VKSVSKKGKKIAKSIEIELIYEDDEPKVRGAERISKFSKRVYARAKDLRPVKNGYGLL
                     VLTTPAGIVSDKEAREKNVGGEALFKIW"
     gene            complement(28502..28687)
                     /locus_tag="VHF05_00990"
     CDS             complement(28502..28687)
                     /locus_tag="VHF05_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HVS79531.1"
                     /translation="MAKKSVVVRSQRTPKYASRVVRRCFKCGRKRAYMRDFDLCRICF
                     RELANEGKIPGVKKSSW"
     gene            complement(29036..29572)
                     /gene="rplE"
                     /locus_tag="VHF05_00995"
     CDS             complement(29036..29572)
                     /gene="rplE"
                     /locus_tag="VHF05_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HVS79532.1"
                     /translation="MTKQKTAKAFESLKESQGYTNKMQAPRLVKVVVSAGTGSFKDKT
                     KNQTVVDRLRKITGQEPSQRGAKQSIAAFKVRQGDPVGYQITLRGARMYDFLDRLLNV
                     ALPRTKDFRGIPSTSIDEMGNYTLGIREHTIFPETADEDIRDLFGLSVTVVTSATSKD
                     EAKAFLEYLGFPFKKDSK"
     gene            complement(29588..29857)
                     /gene="rplX"
                     /locus_tag="VHF05_01000"
     CDS             complement(29588..29857)
                     /gene="rplX"
                     /locus_tag="VHF05_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HVS79533.1"
                     /translation="MKIRKNDNVIVISGKDKGKTGTVVTAFPRENRVIVAGVNMRKRH
                     EKTRRQDQKGKIVEKEAPISVSNVMLLVNNKPTRLNKARGSAKAK"
     gene            complement(29871..30242)
                     /gene="rplN"
                     /locus_tag="VHF05_01005"
     CDS             complement(29871..30242)
                     /gene="rplN"
                     /locus_tag="VHF05_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HVS79534.1"
                     /translation="MIQPRSIVKIADNSGGTVGRIFKVLGSSKKRYARLGDIVVLSIQ
                     KAQPRKQIKKKAVVYGIVVRSKNATRRKDGSYVRFDENAVVLVDKEKQVPIAGRVFGP
                     IPREIGEVGYQKIISLAPEVV"
     gene            complement(30329..30586)
                     /gene="rpsQ"
                     /locus_tag="VHF05_01010"
     CDS             complement(30329..30586)
                     /gene="rpsQ"
                     /locus_tag="VHF05_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HVS79535.1"
                     /translation="MKKDAEQQNSRKKRLEGVVVSDKMKDTVVVLVERYVKHPKYGKF
                     LKRAKRFKAHDAGNTRKVGEKVTIEETAPISKEKRFKIVTE"
     gene            complement(30589..>30771)
                     /gene="rpmC"
                     /locus_tag="VHF05_01015"
     CDS             complement(30589..>30771)
                     /gene="rpmC"
                     /locus_tag="VHF05_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HVS79536.1"
                     /translation="LKNKTEQELKGLITERREALRTFRFDIAGSKARNVKQGRAIRKE
                     IAQILTELNARKTAAK"
BASE COUNT         6693 a         8380 c         8124 g         7574 t
ORIGIN      
        1 cgcatcctcc acacaaaaaa agaaatcgag gaactcggaa aagccgaggc tcaaaaggga
       61 ttgacaatag tgccattttc gctgtataat aaaggacgga aaatcaaggt tgaagtgaat
      121 atcgtccgcg gccgtaagaa gtacgacaaa cgcgacgtct tgaagaaaag agataccgag
      181 cgggaaatac gtcgggaata ctcggacaga tagttgtttg aaaaacagac tcaaaggtca
      241 ttcacctaaa gcagggaaac gcgagttttc agttgctagt ttctagaaac tagcaactaa
      301 tcactagcaa cttccctttt tcagggggat gatcggccta gacaatcggc gagctctcat
      361 atgcgcaaag cggagcattc ctctaatctc cttaatcctc gaggaaaaaa taactgcaaa
      421 caattctgtt gcatctgcta agtctgtgaa gtctccgtac ttcgcgtcta acgatttggc
      481 attcgcctac gcattagcgt aagcgacgcc gcatattccg aagtctgtaa gggatacgta
      541 ggtggaataa ttccggacta ggacaggaag gacacctcgc ctttcttcca aaccaaagag
      601 gctaacgatt gcggaatttc gttcgtttaa tacccgcaat cgctacaact aagcgaacta
      661 tgctttgtag acccatatgt ttctcccttt tgcacccgag ttcgattctc ggcatctcca
      721 ccacttcgct ctgtaagaac agagcttcgc ggtgcaggca ccaccacaaa aaacatcatc
      781 tgctacgatg gtgtttttgt tatacacaag agcgaggtgt cccgcgtagc ttttctttta
      841 aaagcgtagt gggataattt tcttatgcat tattgttata ttttaaaaag cacaaaagat
      901 agtagttatt atattggttc gacccaagac cttaaaaagc gactagaaaa acataatcgc
      961 aaagaggtga catattcttc cactagagcg ccgtttaaaa tcgcatggta tgcagcgttt
     1021 caaacaaaag atttagccct ggcatttgaa aagtatttga aatctagttc tggcttcgct
     1081 tttcgcaaca agcgcctaat ttagcaattt tggagccgca aggcgaacaa aatacttagt
     1141 aactcgtgac caattctccc ttcctctttt accctcccga ctgagtttct gtctctggcg
     1201 cattgcagac cagaacggtc gaggcggtgt cgttttcatc gagaacgacc accgtcacat
     1261 gatctccgat cttcaaatcg gattcgggta tggggcccgc tgaattcctg atgtccgttt
     1321 gagccgtaag attgatggtt tgaatgacgc cgtcctgccg tttgatggta atcgcgttgg
     1381 cttcctcatc aacgatctct cccgtgccgc ccgtgcctcc gcacggatcg ccgctttgag
     1441 gaattgcgcc ttcttgctca ttgcccgcac ccccaaactg accggcgtca ccgccgtctg
     1501 gaactatcaa cggttcccca tgcggataaa tagcgtagaa cgcacctgcg gccgccccaa
     1561 taactacgat tgccgcaaca attatatgta cgtatttcct ctcgcgcacc cactttgcga
     1621 gaatgagata cgccgcaaag cccaccataa cgccaagcgc attgagtatc acgtcatcaa
     1681 tatcaaagat gcctacgcgg agcgccgttt gcatcgtttc gatgagcaag cccgcggtga
     1741 cggcaaccgc gagggtcttt ttccacgtta tattccgaaa tgcaaacgag agaagaaacc
     1801 ccaatggcac aaggagcgcg atgttgccga caagattgat acccgcgata atccatccct
     1861 tataaccgag gagatatggc aagatagtct tgaacggcac gaaatttgcc ggtttgcctg
     1921 attccgttcc gccaaaatta agcatcaact gccctaccct gatggccggc acagccttaa
     1981 acaccatcac cttgatcaag acggtaccgt aggcgatgag aaccagtgct ccgatcaaac
     2041 gcttcttcat atcgtaatga tatcagttcc tgatatctcc ttctttactt ctggtttgtg
     2101 aaacagaatc gtataaattt tattgcaatt caaacgaatc gatcattcgc tcagcaacat
     2161 catcattaag aggataatgg atcattattt tttcaccgga tttgttttcg acgaaaacaa
     2221 agttaaacac agtcccttct tttcctaaac tgtatcctcc agccaaacct tggctgaaac
     2281 ttttagttaa cgtgaatgaa taagcttttt gaccttcaag atctatttcg tttaaccccc
     2341 ctactttttt gttttttgta tagggattgg tatcatcaag ttgcatctgt cgaattgact
     2401 cagcaaattg aaaaagtgga agcatgataa gtttatttcg ttcctcagtg agtgcttttg
     2461 gtgcattttg aaaaggtaga taaattgttg ccccaaactt ttcccccacg cctggtattt
     2521 ctataattat atcatccgat tgttcaacag gtttaccttc catttctgct accggctgaa
     2581 ctgcggcatc ttccgggtat gacactgcat acccatgtgc aacgttctga tatgttttcc
     2641 aatcctgagt ttgcggtact tcagacttta agagaacacc atttgtgcct gggcctccat
     2701 gccatgcgag caatccgagt gaaatgactc cgagtaaaat tatgagactg agtacgattt
     2761 tattcttctt attcatggaa aaagtatacg caaactacat agcagaagca attaaaaaga
     2821 gcgacaggga tataccctgc cgcgtttttg ataccacact aataagtgcg atttgttgaa
     2881 tgatagtaac gaattctact ggtggggaca ccgctactat tcactgcaca ttctgtttgg
     2941 aagctcttca tgagccagcc gtccataaca acatatgaaa gttgcgatac acttgagctg
     3001 cctgaatccg cataccgaat accgaagtgc aggtgagttc cggttgttgg attgcccgtg
     3061 ttacccatgt acccaagaag atctccttgt gcaacagtac tattttgaac tactacaaca
     3121 ttagactgaa ggtgaagata gcgagtggaa aatcctgtgc caagatttcc atcaccatca
     3181 tgatctatca ctacgtaata cccattcgca tcggtatacg aaacagtttt gacatatcct
     3241 ccagctgcgg caatcactgg tatattgccg gtgtacgatc cgccaccatc tttcacattt
     3301 gtaggtgaaa tatcaatggc gtagtaattc gagccttggt gatatggatc tggccatggt
     3361 gattcaccca tacattcgta cccaccaatt tcatttgtaa gcagccattt cgcaccccca
     3421 ggcaccggca tcttgaaact tggcattcca gagggttgtg tgaatgtgaa ttccaaatac
     3481 ggccgatcgc catcattagt ggtatatcgg gagctccgta atacatcaaa gttgttattg
     3541 ttgtattccg gagaaagtgc aattccattg tttgttactg tgccgttttt ccattctgca
     3601 taccaagggg taaaatctac tccccaccaa ccaccggcaa ttggtgcatc gaaccaacct
     3661 gcacaggtga ggaacgaagg tcgcgctgac catgtgagtg ataaattcca cgaaccgccg
     3721 actttgcaaa aagctagcgg cgtagtggtt gaactgtccc ctctgttgta agccttaaac
     3781 catattgtta ccgctgatgg attagcaggc aagccattga ggtcgaattt gatataggtc
     3841 tcgtagacat ctccccaacc acccatctgt agtttgtcat ctctttgaag agtctgacca
     3901 tagtaatagc ttggcaacca gccgtcccaa agatggattt tttcaagatc ctgaggcatt
     3961 gcactgccat cagacttctt gagtgggaaa ggagttagtt ggtaatacgc agtggtttga
     4021 gcgtctgctt gtgtaactac tagcaagcag aaaagaaacg cgacagataa agcagctttc
     4081 attttgtaca accttcctat aaaagaactt cctatgcata aagcatagga ctgtaccaat
     4141 tttcttgaca cattcgagag ataattttac tctactttgt ttagctcatc aatagagtct
     4201 tgagttgaaa acatgaagac agcgaaaaaa accgcccttt tggacggttt ttctctaaat
     4261 ttccaaagtt tgattttcct attgtcccga tgcggcggga accttcggcg tagttgccgt
     4321 cgggccggtg atctcgaact tcaccgaatt gctcattgcc tgcatgttcg cgaccgagac
     4381 ttcatacgtg cccggcttaa agagcatgta gatcattgga cacatcatgc cggggccgca
     4441 gtagacgccg gagccctccg gaaccctgaa cgaaagcgtg gtgctcgact cgacgtcgat
     4501 atcggttacc tggccggtgc cgaatttgac gcgcgtggtc gacgcgaagc cgctgccggt
     4561 aagcgttact ttggtgccga tagggccgaa gagaggcgac atcgagacga tggtcggcgc
     4621 ggtggtcgtg ccgttgccct gatcccctcc cggcatgcag gggaagatga gcctcaggtg
     4681 ggccaaggtc gaagagccga caaagcctgt gccctgcgag agtccgacgg gcgcgaggat
     4741 gtccgccttg tatttgttct ggaacttgat gaccgcgcga agcgtcgcgg ggccgaagta
     4801 tgtggtttcg ttccccggcg atcccgctcc cgacgtggct acctgggtgt cggtagcgga
     4861 attgagcatc atctggagct gtttgacgtc gtcgccctgg agtccagctc taaggttacg
     4921 cgagagcgcg aagcacggcg cgtggatcgc gatcggcaaa atggttcccg gtgccccttt
     4981 cagattcagg ctcgccgcgc ccgaggagct tgccgaactc gcagtttggg cattcgcaaa
     5041 cggcacgacc gccgagaaag cgagcgaaag ggcgactgcc ccagccgcaa aggttttgta
     5101 attcatatgc acgaaaaaat aggcttctac acctgtatag acgcacggcg agcgcaggag
     5161 ttacgaacgt gccttttgac aaacagattc aatgtggcaa catcaccaca ggagaacatt
     5221 catgacggaa aaagaactca cgctggttaa agacggcaat tggaaatact ctcccgatga
     5281 ctgctatctc gagagatccg ctccaggcag gcggcgccaa cgaatctttc tcacttccaa
     5341 gcccgggaaa tatcgcctta acgcttcaaa cggcttcgaa ggcacccttc tccgagtctt
     5401 gcagcacgcg aattcggtgt ggaataaccc tcggcgccgc aaaagagtga aggcggcacg
     5461 catcagagcc gcggaatgac ccgcggcttt ttagtattac aacacgattt ggaaacactg
     5521 caataacgaa aagtggtaaa ataatgcggg gaggaaaaag tgcaaactct agagtttttg
     5581 gacaaagcgc tccggccgct tgggctggaa atccgggagg aaaacgggtg cctgatgctc
     5641 gaacgccaga tcaccaaaag atggtggaag ttcagctttc acaccaataa aagagagtta
     5701 atcgggcgta tcgcccaaat cgacactgac cacaacggcc gcgtctatga aatagacctt
     5761 gaccagtgcg tcgacgaccc gcaaatcgta aagaaattcc gcagagttct cttcagtgag
     5821 tacggaatct taaccctact acactgaaga acggccgctc ggatgccgcg acgctatgtc
     5881 gcggcatttt catatatgcg aaagccgcgc gccattggcg cgcggcttca tgctatttct
     5941 tctcttgagt gtcgcagaaa tgcgcaatgg catccctcat aaataccaca agtccggggc
     6001 gatatttgtc ataggtcgcc gtaaagcggg gatcgtcgac gtacattttg cccaagttgc
     6061 gatacattgc catcgggcag tcgtagaagc gccggatgcc ggcatagtgc ttcgcgataa
     6121 gcgcctggac ttcggagctt tcggttccct gatccatcgc tttggcaaga gcttgattga
     6181 gctcttccgt ttcccgcttg atcgcggcga gatcgccctt gctcatcttg gctacgcggg
     6241 cagtcgactg cttgtaggcc tccgtgtgcc cccacctccg cttcacttct tccgcatact
     6301 ggtcaacttc gtctgtcgag aaggaattgt agagtttgtc gtcttccatt ttcttttttt
     6361 tgctcgtttt atttgcgagg ctgataatcg tccggtcgat ggtcttgaga agcccgccga
     6421 ggcgcttgcg cctcaactcg ataagcttgc gctggtcgcg aagcgcctgg cgcacatcga
     6481 aatccggcgc atcgaggatt ttcctgatct cgtcgagctc gaaatcgagt tcgcggaaaa
     6541 acatgatctg ctggaggcgg atcagctcct tctcgccgta ttcgcgatag ccgttcgggc
     6601 gcacggcgcc cgggttcaag agcccgattt catcatagaa atggagcgtg cgaaccgtaa
     6661 ctcccgccat gtcggccacc tgtttgacgg tatatgtttt catatacgca ctctaccata
     6721 aactattaca ttacgtaata gtcaagcaat tactcctttc attccccacc ggcgtctaca
     6781 gcgggtgtca aaaacgctct gacgagccag taacggaaaa cacacttcct gctcgcacta
     6841 cgtactgaaa aactcccgct gtcgggagtt tttcagtacg tagtgcatcc tgcagaattc
     6901 gaatctgcga cctcacccgt gtgaagggtg cgctctaacc agctgagcta aggatgcatt
     6961 tttcgttcgg gctacccggt aacgctccgg ggcctcatgc accccatgca tgtatactgc
     7021 ttttatacta tagcccgttt tgtttttgat aatccaaccc tcacggataa ttttctggtg
     7081 aaaatttccg cgagccttac aggctctgtc tactcattcg ctctcgagga ctcgagctga
     7141 atgagtcttg cggaagctgc ctatcgtcgc ttcctcaccc gcctcggctc agtcagcctg
     7201 cggccttgcg aagcttcccg ttgtcgcagc tcgctcgggt ctcgcaaatt cttttcagaa
     7261 tttttgcgga agtcccccgg acttccggtc tttgctttgt gggggtgccg agaatcgaat
     7321 actaaatcct tgataacttg gtcggggtgc cgagaatcga actcgggtct cacgaacccg
     7381 aatcgtgtat actgcccctg tactacaccc cgaccaagtt atcaaagagc cgcggtcacg
     7441 cgtatttttc cctgcctgcc ggcagacagg tgctgaaaaa tcacgccgcg ccgccttgcg
     7501 ccagtcgcgc tccgcccttg cggaagctgc acgaaccctc gcttcctcac cccggcaaaa
     7561 ccaccgaaat tgagccttca cggctcctat aagatacaac aatatagaca aaaacaaaac
     7621 tgtccgccaa ttgggggcag ttttgtttga aaagctttct tcggtacatt cttccggaat
     7681 ttattgcacg cttgtcggca ttccggcgcc gccatttgct ccgccgccga tattacccgc
     7741 cggaagaggc tgatttgccg gatacgctcc tccgctcgaa ttcggaacgg cgaagaacgc
     7801 tcccgcgaag agaaagagga gaatgacggc gagaagtatg atgagggcaa tgatccagcc
     7861 catgctgccg cttctctcct tctcagtatg ctcgtgctcg tgaactattg ttgccatatt
     7921 cgtggattgt tacggccgcg tttgtttccg ctgcgacctg ttccgcacta tatccaaaca
     7981 gcatgaaaga aggattaatt ctctccctag agcgtcggac aaactgcctg cggcctcgtg
     8041 cggcctactt cacgcatgcc gaccaaagcc cgatcccatc cgtgcgggcg agagccgcgg
     8101 catccgcgaa ttctttcgcg tgcgcggtat cgggcgggat gggctccgcc gtggcgaatc
     8161 cttccttcac cagctcaaga ttgacgaacg tgccgtcggc gtagacatag cgcagcaagc
     8221 gcccgtattt gtccttgtcc tccgcgtcgg aaacgagcgt cacggccttt cccgacacta
     8281 attccttatt cctctccgac gcctccttgc cgtagcactc aaccggcttg tcgggatcga
     8341 cggtttccgg cgtgtccatg ccgatgtagc ggacgcgggt tttcactcct ccgataagca
     8401 catcgatggt gtcgccgtcg acgacgtccg caacctggta cgtcccctgc atcggagcgg
     8461 tcgtggtcgc ggcgccgtcg cggtagaaaa tattgccgtg gataaaatca aggtaccaca
     8521 gtgagccata gccgaggatc gcggcaagga tcgcgatgat gaaaagtata aaaatcgcca
     8581 ttcgcgcgct catgcattca ttatatatga ggattatttg ctggactgcg cctgcgggca
     8641 cgatacaata atattcataa tggctacgtc gaagaaacgc ggaaagatcc accgcctgag
     8701 caggcacgga gcatatctca tcgtgggcat cggctgtctt ctggccgtcc tctgcggcgt
     8761 gctcattctc tcgaagcccg acattccgtc ggaggcgacc gacggcaagc aggccgcgaa
     8821 cgtgatcgtc gatcctggcg catcgcctca gcagatcgtc ggcaatctag cgaaccgcgg
     8881 cctcatcaag aatcccctct ttttccgcgc ggcggtggcg atcgcgagcc tcttccataa
     8941 ggtgcaggcc ggcggctact acatcgcgca gggcatgagc gactgggaca ttttccgatc
     9001 gctcgcgaac ccgtcgctcc gctatgtgac gatcgcggcg ggcatgcgca agcaggaagt
     9061 ggccgatctt gtcggcgaca agctcgactg ggacaccgtc gagaagcgcg agctcgtgaa
     9121 tgcgaacatg gatctcggcg gggacgatct cgaaggcaag tatttccccg gcacgtatct
     9181 cttccccgac gacgcgacgc cggaagaagt cgccaagcag atggtcggca actacgaaga
     9241 ggagatggag aagatcaagg ccaagtaccc gaaatcgacg atcaactacg ataccgcgct
     9301 cctcatcgcc tccatcatcc agcgcgaggc cgcgggcaag caggacatgc gcctcatctc
     9361 cggcataatc tggaaccgca tcttcgacgg catgagtctt tccatggatg ccaccgtcca
     9421 gtacgcccgc ggcaagaccg acgacggctg gtgggcgccg atcacagctt ccgacatccg
     9481 caccatcgat tccccgtaca atacctacaa gaatgatggc ctgccgccga ccccgatctc
     9541 gaaccccggc cccgacgcca tcgacgcggc catgaacccg caaaagacga gctgcctttt
     9601 ctacatccac gacacccatc acgtcatcca ttgctcggcc acctatgcgg ggcaattgaa
     9661 gaatatcaac acctatctga aatagcccga aacagggcca ttttccggaa atccggccat
     9721 tttcgaaaat gcgccggaaa atattgattt atcaatgatt gtgtgctatc tttgtaccgt
     9781 ctgataaagg caatcaattt gagcacacaa gttcgtacaa atgagcagat acgcgcggca
     9841 gagcttcgcg tcatagggcc tcagggtgag aacctcggca tcatttccct cgccgaggcg
     9901 ctccgccagg cccgcgaagc caatctcgac ctcatcgaga tctcgcctgc cgccaatccg
     9961 cccgtcgcga agatcggcga ttacggcaaa ttcctctatg acgccaacaa gaaggcgaag
    10021 gagtcgaaga ccaaggcgca cgtcatcgag gtaaagacca tccagatcaa ggtcggcacc
    10081 ggcgagcacg acctcgcctt gaaggccagg aagatctccg aatggatcgg cgaaggcaac
    10141 cgcgtgaaga tcgacctttt ccttccggga aggaccaagt atatggagca gaagttcctg
    10201 gaagagcgcc tggacagggt gctcaagctc gtgagcgagc cgtacaagat tgccgaccca
    10261 gcaaaaaaga gcccgaaagg cctcacagta atcatcgaga aagatcgata acatgaaaac
    10321 gaataaatca tacacgaagc gcttgaaggt gacgaagaag ggcaagatca tcgctcgcaa
    10381 gccgggccac gaccacttca atgccaagga gagccgccgc aagcagctca acaagcgccg
    10441 cggcgcgacg ctcacgattt ccaacaaatc gcgcgcgcgc ttcctgccgg gcaactaata
    10501 attaataatc tagactgaaa tgacacgcgt taagaaagga gtgaatgcgc ttaagacgcg
    10561 ccgcaatact ctcaaggcaa ccaaaggcta ccgtttcggc cgctcgacca aggagcgcca
    10621 ggcgaacgag gcgatcgtcc acgccggcaa gtacgctttc gcgcaccgcc gcgacaagaa
    10681 gaacgacatg cgccagctct ggaacgtccg catctcttcc cttttgaagg cggagggcac
    10741 gtcctacagc aagttcatcg gaggactcaa gaaggctaac atcgctctcg accgcaaggt
    10801 ccttgcgatc ctcgccgctg agaatcccga aaccttcaag aaggtgcttg ctaaaattaa
    10861 gtaaattagt aactagtaga tagtagctag agaaaatcgc cccgtggatg tcgcgcttcg
    10921 cgctatctca cggggcggtt ttgtctaagt tattttaaga gaattgaatt gaccccttct
    10981 aattactatt tactaatatc tactaagtac tggctactct ctacttcagc tcataaccct
    11041 tccccgcttc gggaaagatc gcgtcgacgc cgacgtagtc gcggaggcgc tgggcgagaa
    11101 acatcgagga tttcggctcg cccatgacga cgaatacctt tttgagcttt gatttgcctt
    11161 cgtcgacaaa ctcgaggaga tggtcggaat ccttgtggcc ggaaaatcct tcgatcatgt
    11221 cgatctgggc gcgcacctgc ctttttcggc cggaaatcca cgtctccttc actccgtcca
    11281 gaagcctgcg ccccaaggag cccggcgtct gatagcccac cagaagaatg gtgcttttcg
    11341 gatcctcgat atgatcagct tcgtgggatt ggatcctgcc gcccatagac attcccgagc
    11401 cggcaaggat gattttcgga ttcgcggtct tctctatcgc ctccgattgc tcccgccgaa
    11461 gggtgaagga gagctttggg aaagcgaaga tatcgtctcc cgatgcggcc acccccttcg
    11521 ccgtgtcgtt aaaaaggttt tcgtaccgct tataaacttc cgtcaccttg atcgcaagcg
    11581 gcgagtcgac gaagacgggc accgagggga tcttcttccg ctcgaaaaga tcgttcaatt
    11641 catagaggat catctgcgtg cgctcgagcg agaatgcggg gatgaccaag gcgccgccgc
    11701 ggcgcatcgt ctcattgatg acgcgctcga gcttgtcgat gcggctgtcc ctgttctcgt
    11761 ggttgcgatc gccgtagacg ctctccatca ccaggtagtc ggcaccgtcg acggaatcgg
    11821 tgtcgcgaag caagggcgag ggcgaattgc cgagatcgcc ggtgaacacg acggtttttc
    11881 cgcctttttc gaaaaaaaac atcgtcgagc cgagcacatg gccggcatcc ttgagtgtta
    11941 cggtatagcc ggccaattcc gtcggaacgt aataggaaac ggttttccac atcgaaaagg
    12001 cagcatcgat gtcggcctgc gaaaaatacg gctctttgcc gaaccgcttc gcgtcctccg
    12061 ccattatcgc gaaagcgtcc ggcagcatga gttccgagag gtccttggtt gccgggtgcg
    12121 aatagatgac gcccttgaag ccgtcgcgaa cgagcttcgg gatacggccg acatggtcca
    12181 tgtgggcgtg cgtgacgaaa agaacgtcga tggacgccgg atcgtagccg aacggcttgg
    12241 cgtttatgtc gaattggtcg gcgctgtggg ctccctgggt catgccgcaa tcgacaagca
    12301 cgcgcgattc tccgttttcg aggagaaaat tcgcgcccgt gaccgagccg actccggagt
    12361 agaacgtaac tttcattatg cagaaactat atcatgcccg agcttcccgc gacaaaaata
    12421 tgcaaaaatt ccgtaccata tggtacggaa tctttggcgg agttacatca aatattgcct
    12481 ctcgttaaac gaggtgggtg cccgcaaccg cattcttttg aaatctcaga ccggagccgg
    12541 agttctcggc agaaggaagt tatagtgggc tccctggcag tttgttaagg agaaaatatt
    12601 tgaatataac tcctgcttat actatatccc cgctttgtga aattttcgag tagaaatctt
    12661 aagatgattg tgttgtatta aatttccaat ttttaatttg caattttcaa tgaattttca
    12721 aatttcaata cttcaaatgt ttgaaaattg tttcattgtg ccttgattga aaattgagaa
    12781 ttgtaaattg aaaatttatt ccaaaatgcc gtcgagattc acgtcataga ggatttcctc
    12841 ctgcacgagg cggtaattcg tcacttcctc cggcttgaac cagtgggcga tttccatgtt
    12901 cgcctcctcg acggagccgg acgcatggat gagattgcgg atggcgcgct tgtcgccgtc
    12961 ggacatctga taggaatcga ggacataatc gccgcggatc gagccgacat ccgacgagag
    13021 cggctccgtc gaaccgacga gcttgcggac gatcttgacc gcgtgcgctc cctcgatgac
    13081 catcgcgatg accggccccg aggtcatgta tttcttgaga ttttcgagaa tcgccgcggt
    13141 gatggcgagc gggtcctcgg aaggaggcgt gaggcccttg tcgcggtagc ccttgatggt
    13201 tttctcgccg gtcacggtgc gccagttggg atcgagggta tagtgcttct cgacgtgctc
    13261 gggagtagtg acaagcatct tcattgcgac gaacttcagg ccgacgcgct cgagccgtcc
    13321 gatgatctct cctacgaggc ttctctggat gccgtcgggc tttatgatga caagagtcct
    13381 ttcgtctttc ggatgcatag gatttaaatc ttaatgttgt gaagcagtat agcaaaaagc
    13441 ccccgcaggc gcacggagac tagggacggc tcgcgccgag agacgcttga ggctctgccg
    13501 gcgccttagc atactttttc atgatctcgg cttcgatcgc ctcgcggtcg ccgccgtact
    13561 tgagatacga gagctgtttc agctcggcaa cgtgcgacgg gtcgaatttc tcgggaggcg
    13621 tcacttcgat attgaagggg cggcgcggct ggccgttcgc cagaagcctc aggtacgcgt
    13681 tgtgattgtc gatattcatg aggtcgttcg cggtgaaaac cggctcaaac tgcttcacga
    13741 gcgcctcggc atcggccgcg ccgacgcgga acgccgccat ggagccgacg ttgccgaaga
    13801 cggagttctt gatcccctct tcaagctggg cgatgaactg gtgggcgatg gtgagcgaga
    13861 gcttgtactt gcgcgcctcg gagaggatgg tcgaaatgga gttggtggtg atgttctgga
    13921 attcgtcgat atagagatag aagttgggga gctccgtgcc gaaggagtcg acgcgggaaa
    13981 gcgccgccat gaggatcttg ccgaccagga tgaggccaat caggttcgcg ttgatgtcgc
    14041 ccaagcggcc tttggcgaga ttgacgagaa ggattttctt gccgtccatg atctcgcgga
    14101 agttgaatga tgatttctcc tgggcgatga tcggacgcat gatgtcgttc gcgaggaaaa
    14161 cgtcgaattt cgaggtgata tacggcacga tgttcgcgag cgagccttcg ccgcccgctt
    14221 tctcggcgat gccccgccaa aattcgacga cgatcggatt tctgcacctt gagagcttca
    14281 agtcgcgaaa cttcttgtcg gagaggacgc gcgagacgtc gaccagggtc gagccggtct
    14341 ccgggtcctc gatgacgagc atcgtcgcgt tgcggaagta ctgctcgaac atcggaccca
    14401 tcgactccgg cacgccggag tagagcttct ggaagatgct gaagagctcg ttcaccacga
    14461 aggtcttctg ttcgggaaag cggcgatcgt actcgagcat gttcaaggcc atcggtcggg
    14521 ccgtttcgct cggatcgaag tagatgacgt cctcgtagcg ctcccgcggc acggccgcga
    14581 ggatgtcctg gatgtccgag ccgtgcggat cgatgtagca ggcgccgcgg ccttcgcgga
    14641 tatcctggat gatttggttc ttgatgaagg ttgactttcc ggttcccgtt tgtccgatga
    14701 tgtagaagtg gcgcaagcgg tcctcctcgc tcataaggat gcgggtctcg acgttgcgat
    14761 ggcggttaac tcccaaaaga attcccgttt ccgaaaggcc gatcggcgcg ggggcagatc
    14821 ctgccttggc gcggcggagc gccggcgcgg catccgcgcc ggatgtcgga aagtgtatga
    14881 gagtcgtaag ctccttgatg ttgaggggaa tgttctggtg ctgggaatgc aggcggtacg
    14941 agaattgatg gaggaggctt ttgagcgctc ccttgctgac gcggttccat ttgagcccat
    15001 tgcctttcgt attggaaaac tggttgaacg acgattccat gtcggagagt atcgcctcgg
    15061 ccttcatcct gttttccgcc gagacgacaa ggcggatgtt ggcattgaaa atcgagctcg
    15121 caaccttgtt ttggattccc tgaagctggt cggggtcgat cggcgtattt tccttggggg
    15181 ttccatcctt tttcttgctg gttttgaaga aatccttgac ccccttcaag agatcgccgg
    15241 tcatggagta cgagatgtcg atcgcttcct tgtaattctc gcctttctgg atgcgcttca
    15301 gggcgtgctt gtagcgcaga agaacatcgt cgtcctggtc ggggctcatg acgatctgga
    15361 tcgcggcgcc ttcgcctaca tgatcgatct tcgagaagct cgagagaagg gcgttcagcg
    15421 gatcatggtc gaactgatca tagagcgtta tgggaaagac gggattgccg gccgacttcg
    15481 caaacgacgc ggccgattcg ccttgcgggt tgaagatatt gtagtcctcg atcttttcga
    15541 cgagcttcgc atgcggaaag acggaaagga tgtgcttctc gaaaaggagc ttcttgtcga
    15601 cgggaacgcc ggcataaaag gagaactccg cgccgatgtt ttccatggcg agctcgatcg
    15661 tgaagtagtt gtggccgcgg ttcttctccc ccggaatgga gcgcatgccc gcataaaact
    15721 gctccatgcc ggaaataagc tccttgaagg acccttcctt gtccttcccg ccttccggaa
    15781 tcatgatctc gtagagcgtc atatggagcg agcggtagag cggcgatttc tccttgagtc
    15841 cgggcacgac gaagccctcc ttgatgtact cgatgagata gcgatggaag tcgtcgtcga
    15901 gatgggggtt cgaaagcttc tcgaggagcg cgaaggcgtt cttgatgccc ttttcgcgga
    15961 gcacttccac gagctcgtcg atctgcgcgt cgtgcgcttc gggagggaga ttcaatatga
    16021 tgcggttcag gttctcctgc gtcatcttga gatcgggatg aagcacggtt tccggcgctt
    16081 ttttgtattc gtcgacgcgc tcgttgatga tctcgtgaag ctgcggcttc tcgccgtgag
    16141 cttcgagctc gcgttcgcgc tcccgcacct gcgcccgcag gtaatcaagc tcctcctgcg
    16201 gagtcttgaa ctgcggcgcc tctttgaaga gtgggccttc cattgaataa atcaaaaatt
    16261 aaaaatcaaa atgcaaaatt aacagtccta ttctccccct ctgtcatgcc cagcagcctg
    16321 cctgccggta ggcaggggca tccagggctt cggcaacaat actctggatc ccccttgctg
    16381 gagacgaaga acgggcgatt tgccttccgg atattatctt atctttcatt atatctcagt
    16441 cgggagattt tttactcaca aaagaaaatt cccgctgtgc gggaatttct tgataaatat
    16501 gggaataaat attaaccgag gatttcgata tcgccatctt tgaggtcttt ttcgagctgg
    16561 gccttcattt gctcgggaga gagattttcg tcgcccgact tttccggctg gccgttctgc
    16621 ttgtccttct catccgcctg tgggttttga tcgtctgcca tgatttgaaa ttataccaca
    16681 cctatctcct gtaaaaaggc catctggtag gaggcggggg cggtggatat ccatagcggt
    16741 gaagcggctc ggcctctatt cgggccgtat tttggaagga tttcagaagg cttttcccga
    16801 atccgtcggg cttcaggcgg ccgcggatga actcgtcgag gggcattttg agcgcatcgt
    16861 tgtcggccgg agacacgtcc ttggcgtcga gcttttcgta tacccaatcg acaaaggggt
    16921 tttccgtgcc atcctcgtgg ttcgcgatat atgtacggcg caattcgccg acagtgtggc
    16981 tggattttcc ggcaatgtga ttgcgatacc actcgaggcc gtatccggca tgctttccgt
    17041 ttttatattt caatccctgc aagacatcat tgaatttttc gaccgtcctc tgatgcgtcg
    17101 caataatctc gcgcatcgaa gcattgatcg acagaaattc agtgcggtcg gccgggttgg
    17161 cgaacatgcg catgcgcgga aactcccgct ggatcgtcgc gacgtgcttc atgacttcgt
    17221 cttgggcgag aagcggctgg tcgtggatat tgcgcgaaat atatccgagg tcttcggcga
    17281 tgacatgtgc gttctcgggc gtaattccct tccccgcaaa ctgatcgagt gcttcggcat
    17341 gcgagaagac tttggcggcc ttttcatatt ccttgccgcc gagaatgcgg tgaagatcgc
    17401 caagcacggg atcatgcgaa ggctgaagat tttcaagcgg agtctgattt aagccgccct
    17461 ggggcgatcc aatcgtcgaa tcgttggaaa tatcgccgtc gttgtgaact tccggctgaa
    17521 tcggcgcgtt gccgccctcg tgatggacgg gaggagcgtg atggcctctt cccattccct
    17581 ggcgcatccg gtcgacatcg ttcgagatat cgccgtcatt atggagttca tgagacggta
    17641 ctgcgcggcc agttgcctca tgcgcagcat gatccgcggc accttgctcc agtccgacag
    17701 tggatcccat ttcatgggaa agaatcttgc gcataccatc cgcacgctcg tttatgatat
    17761 ggagcaattc atccgtctgg ctgtcgaaac ggcccgtcgc gccgctcgtg atgagcttgt
    17821 cgtggagatc ctggacgcgg acgaggattt cctgctggag ctcaggcctt tggccgccga
    17881 agctcttgga gaggaactcg acctcttcgc gaagatgccg catgtcgggg acctgggcgt
    17941 acatgccttc atgggaaatc tgggacgcgt actgatcgaa atcggcgaag tgggacgtca
    18001 tctgctcggg gctcgcgttg ccctggtagt agatgctcag ttccgtgccg tcccagaacg
    18061 gcttgtgcgc tcccttcacg attccttgat cgagcggatg aagcgtaaat ccttccttcg
    18121 gagcggcagg ggccgatgga ggaggcggaa cggcatgttc ggcattcgga gcggaagggg
    18181 gcggcggaac ctcgtgggcg ggggccgaag gtgcgggagg aacagccgca tgcgccggag
    18241 ctggcgcatg agccgcggca tgctggtgcg ccaccattgc agccttgagc gccttttcgc
    18301 ttttcgcttc ctccgcaagc tggttggaaa gcccggtctt aaccttttgg aactcctgct
    18361 ccatagagct cagccgatcc ttcaccgaat tgaattctcc ctctgtccca tgatgctccc
    18421 aaaggaggtt gtgagctacc tgaccaaggg cggagaggcc gaaggcccag gcggcagatc
    18481 tcatgccgcc gcggcgcgcc tgccatgcct gggcggaatt aatgcgcttc tctctccatg
    18541 aaaggactcc tttaatcttc tttgtggccc gttccgtctt ttccgcaagg tctgtagatt
    18601 ttttgcccgg cctattctgc tcaaacatgg caagaaatgt tcctgcttcg ctcaatttct
    18661 gccgcaattc aagctgttct ctaagacgct ctgcgatctc atcagaaccc tctcccacgc
    18721 caaaacttac caatccgcga ttgagttttt gacgcgtata gaaaagcctg actttgagct
    18781 tgtgcattat cctttcccgc tccgcatcct tttctttttc gctttcgaat ttaggattcg
    18841 aatttacact gtcgaattcc cgttctaaat cgtcgatttt attcacaagc acttccgcag
    18901 gcacaaaatt ccgctccatg tgcgttttcc gttcgaattt cttcgcaagc ctgtttactt
    18961 ttgcctgctg ggcttcaaca tcatttttcg catccctggt gccggtcaac ttcgcgccag
    19021 ccttaagatc acgaagcttc tttttctccg catcgagctc ggctgcgaga gctttgaact
    19081 cgtctttccg caagctggcg cgtgcgcctc gccgcatgtc ttccgcgcgt tcggcctgag
    19141 cgcctcgagt gcgcgatctg ccggtgacaa aacccactcc cgccgcggca aacggcagca
    19201 tcgtcacgcc gcctattgcc attgcggtcg aacgggaaag aaatccggca gcggagatga
    19261 tgccgcgctc ggtcactatg tccttgagca cttttttcca gacctttcga tccttgatat
    19321 tgagaagctc ttcttcaatg tccgaattcg agccgaaaag ctggtttgcg cggaggcgtt
    19381 catcaatagt attgacgtaa agttcggcct cattacggtt gcttccgttt tccaaaaggc
    19441 gaagatcgaa gattttcttc ttcagattcc taaaatttct ttcgaccagc caatatttgg
    19501 tgttttggaa cttcgaagcg ccggaataat tccattcggc gggcgtgcgg gcgaattcgc
    19561 gggcggcttc gttgaatttc gagatgagct cgcgctcggc aggctgaagt tccctgttgg
    19621 caggcgtgag atccttttcc gcaacataat cgatctccac ttggccgttt tcgatatgcg
    19681 cgtcgagacc gagctccttg gtgcctttca cgagctgctg aagaatctcg ccatgggtct
    19741 taaggccgcc ggtgaggata tcgttcgcac tctttttctc aagcttggca atatgataat
    19801 gcttcgtcgc accgagccat acgcgcccga gtgccttgtc gaacttggca aacctgccgt
    19861 ttcctccaag cttcaaaact ccggcatttt caagatcctc tttgaactct ccgcgcgcct
    19921 cttccttgat gcgaccgagg gtaacctggc gaagattctc gtatacgagc cgcttctggc
    19981 cgtcagtgag atccttatac ccttcgatct tgtcgagatc gccgtccttg aagccgaacg
    20041 tgtccttgaa atactgctcc gtgttctttt ccgcgtcgct ccgctccttt cgtgcctcct
    20101 ctttttcggc ccctgcctca gccggttcgg ccgcttcttc tttttttcct tccggctctt
    20161 cttcgccggc ctttttctca gatttctcct tcgcggcttc ctcggcaagc ttcctttcga
    20221 cagactcggc atacgtgtcc ttctcctggc ccattctcac accaacaaga aatttcgtga
    20281 attcctcagc atatttccgc aaatctcctt tcgtgagccg tccgacaaat tcggaatcga
    20341 acaccacgtc gacattcatg ccgttgccga attccacgcc gcgtattttc cgcacctcgc
    20401 tgtcaaaacg ctcgcgatct tccggcttga gtttgccgcg ggtcctctca atatcgtgat
    20461 agttcgaagc agatgccaag tcaaaagcct ctttatcctt ttccggctcc gccaccggag
    20521 cgagcacatg gtatacgatt gtgatttttc gtatatcttc gtcggaaagg cctttttcct
    20581 tccagaatct gacaatcgga tcattggcta tcttttccgc cagatattcg tcgccttcgt
    20641 atttaacagg cactgccgcc gcgcgcatct cgttttcccg cgccaattcc ctttcttttt
    20701 gctctttttc ctcttcttcg cgagttgcct caagatgaag ttgttcgaca ggtacgcccg
    20761 tcgtgccgcc ttcgacaaat gcgagtttgc catcctcgga aaaaccgctg attttgcgag
    20821 gcgtttcaaa ttgatcaacg ccctggcttg tccattgaac gaaatcccct tcctttatct
    20881 ggaaaggttc ctcctcggca tttttttctt cggacgcagt ttcgggtttt gtctcgacaa
    20941 cctgttcgga cgaacttgca gtctcgttcc tcttgatcga agcgaccatt tgaagcaccc
    21001 attcaagatt ccgcgagata gttctgactc cttcttccat acttccatat cctgattctc
    21061 ccatgtctat catgcccttg agccattcgg cattctccgc tatatattct tcaaagtctt
    21121 taagagagaa gccttctttg atcaaatcag agtatttcgt ctccaaaagc tccctgagcg
    21181 ccgacttgat cgcatcgcga tacggcttaa gcttcgcatt ttccgcttcg gcctcatcca
    21241 tctctccctg ctcggcctcg atttcttccc cagtaacttc cggctgtaca gcgggtgctt
    21301 ccgtaagttt cttcttttct tcctcggcca tctgctttgc acgcgtttcc ggatccgaag
    21361 aacgcccttc tttcaagtca gccaggagag cccgtgcatc cttattcact ttgtcggcaa
    21421 aatcctcatc atcggagaaa tactcatcga tccccgcatc tcgatacgcc tcgccaatga
    21481 gagtgcggag ccgttcagct tcatgtttcc atctttcatg cgtttttgga aacggcgttt
    21541 cttcaccctc tgcagtctca cgggaaatct cgccgcgagc gatctcatta attcttttct
    21601 gaatagctgg atcatttcca atctctagcg tttttgccag ataatccaca ccatcttcat
    21661 tcgcctggtc ttgaaactga tcaacccagc ttcgaatcgt ttcctcattg tacggatcct
    21721 ctggataata ggcctggaac aacactgctg cgatatcaaa tttctgagca tcgctcagtt
    21781 gtccggcttc aatagcctta acaaccttat ggagtgtata ggatccgaaa ccatgctcgc
    21841 cttgcaattt tcgccttgtt tttggatcaa gcctgcctcc cccttctttc agagcctgcc
    21901 ttacaacatc gatcctcggg tcattttcgg gacggtagtc gggattttca cccggcttgg
    21961 aaccaagagg catacggcgg cgcttggggc cttcatactc ttctccgcct tcattatgtg
    22021 gaaaattttc ttcgctcata actcccgcgc cgaagcgctt aatgagcaaa ttatatcacc
    22081 ctgtagtaga ttttggcatc gcgaattgaa gacaattcgc ggtcgagaca aatgccaaaa
    22141 ttcactacag ggtatcatca aaatatggaa attccggaga atcagcgggc ctgcccggta
    22201 gtgaaaaatg atattaaatg ttaagcaagc atgcgcatgc ttgcaatatc attttctact
    22261 accgggtcaa aacctcgact ccgtcctccg tcacgagtac ggtgtgctcg aaatgggccg
    22321 cccggctgcc atccttggtt cgcaccgtgt attcatcgcc ctcgaagacg actcggccgc
    22381 ctccaagggt gagcatcggc tcgatggcga gaaccatgcc gggcttcaag gccggccctt
    22441 gccccgccac gccggtattg ggaacgaacg ggtcctcatg gaccgagtag ccgacaccat
    22501 ggccggcaag gtcttcggtg atggcgtagc cttcctcctg cgcaaagatt tcgagcgcgg
    22561 cgccgatgtc gccgatatga ttgccgggtg cgacggcctt gatgccgcgc tcgagcgctt
    22621 cgcgggtcgc atggatgagg cggcgcgagt cgtcgtcgat cttgcccacg ccgagggtga
    22681 cggcggcatc ggtaatgagc ccctggtgag tgagcccgag gtcgagcgtc acgatgtcgc
    22741 cctcctgaag gacccgcgga ttcacgttgg gaatgccgtg cacgatgatg tcgtttatgg
    22801 aaacgcagag cgccgccgga tacgggcgct tcacgccgcg cgggcggtag tttaagaacg
    22861 cagcggcgtc cccgccgtcc tcgatcatct tgagcgcggc ggcattgagc tcctcggtgg
    22921 aaacgcccgg ctccgtcatt gcggcgagtt tgcggagcat ctcggcgtgg cgcctgcccc
    22981 cttcgcggag cttctcgatg tcttctgctg tcttaatggt gatcatagta tggaattcaa
    23041 aagtcaaaat tcaaaatgca aaagtttaaa tcaaaattgg aagacttgat aatttacagg
    23101 ctttggaatt aaaattttga cttttgcctt ttgaattttg cattgtcgtc gctaccagtc
    23161 gattgcggag acgatatcgc tatggaccct ttcgatctcc tgctcgccgt tcacgtcgat
    23221 gacgctgtag cggccgttgt tgcgaaaata ttcgatggcc ggcacgacgt tggtatcgta
    23281 ccattcgacg cggcggcgga tgtcggcgtc gttgtcatcg gcgcgggcgc ggcccttcag
    23341 gcgctcggcg gcccactcgc gcgacacgtc gaggacgatg atcgtcggat actcgcggtt
    23401 gtagaagcgc agggccgaat cgaggatcgg cgcctcgtgc gggcggcggg agaggccgtc
    23461 gagcaccaaa tgctcctcac cggtgaaatg ctcgatgagg tagttcgacc agatccaaat
    23521 gggcatgaat tcgggcagaa gcttgccgtt ttccacgata tcggccgtga gcttgctcga
    23581 atagttgtcg tgcgtcatga aatcgcgaac cttctggccg gtctcgatgt agagcgtctt
    23641 ctttatcggg tcgtgatcgc tgatgaactt caaaagaagc tccgcctgtg tacccttgcc
    23701 ggagcctgat cttccgaaca aaataattgt ctgcgctcgc attacacccc aagcgtagca
    23761 tacgtaatgg ctaaatcaaa tttttgagaa gtgcggccgg catgcttaaa taatgagaac
    23821 ttgctcattt ggatgcaatt caaggcgcaa gcaaaatttg cgaccaagct gtacaaagcg
    23881 gtactgcgcg aaagcaaatt ttgcgagcaa cgcagaagtg cgccaaatgt gcagttctca
    23941 taaacatggg taaaaagact atcatcacca ttgccggcga tctcggaagc ggcaaatcgt
    24001 cgaacgccga caaggctgcc tccctcctcg gctactcgcg cttctcctcc ggcgacttcg
    24061 cccgcctcgt cgccaaacgg cacggtatga gtattgaaga gctcaacgag cacgcggaag
    24121 agcatccgga aatcgatcat gagatcgacg acgaggtgaa agcggccgga gagcgcgaaa
    24181 agctcgtcat cgattcgcgc accgcttttc actggattcc ggattcgttc aaggtttatc
    24241 tcaaactcga cccgaagctc gcggccgcgc gcatctacga gcagatgctc cagggccagc
    24301 gcgtgagcca gcacgcggac agtgtcgacc acatgtacga gcagaccatc cgacgcaagg
    24361 aaagcgagcg caagcgctat ctcgcgctct atggcttcga ttccacagac ccctcgggct
    24421 tcgacctggt aatcgacacc gctaccgccc cgctcgagaa agtgtcggag aaaattatcg
    24481 aggaatacaa gaagtggctg ggaagtaacg agtagatagt aatcagtagt gagtaggtag
    24541 ataaagaaaa accgccaata ttagcggttt ctttctactc gctactaatc tactcactac
    24601 tttgctttag tactccctga tggagacctg agcgtcgatc cgtttaatca aatcaacaat
    24661 aacctgaacg acgatgagaa gcgccgtgcc gccgatcgcg agggaggtga tgccggtaac
    24721 cgcgcgcatg acgagcggaa ggacggcgat gagtccgagg aagattgcgc cgaaaatggt
    24781 gagcttcgtg agcttgtcgg cgagatattc cgaggtcgac aggccggggc ggacgcccgg
    24841 aacgaacgcg ccgttcttct ggagattggt cgcaacctgc tcgggatcga aggtgatcgc
    24901 cgtatagaag aaggtgaaga tgaagacgag gacgaagtag atcgccgcat agagccagct
    24961 gttgttgagg atcgcgacgc cgtgctgggc aatcgaggca agcaatgaat tggtcgaagc
    25021 agtgaggaaa ttgaagatca tctgcgggaa aagcaggatc gagagcgcga agatgatcgg
    25081 aatgacgccc gcctggttca gacggagcgg aaggtacgtc gagacgccgc cgtatacctt
    25141 gttgccgcgc acgcgcttgg cgtaggtcac gggaatcggg cgctccgctt cggtcacgat
    25201 gacgacgccg aagatgacga ccagcgccgc gacgaggaat gccgcgtaga gcggaagctg
    25261 ggtcgcatcg aacgcgaacg cgagctgtcc ggtcgaagcc gggaggcggg cgagaatgcc
    25321 ggcgaaaatg agaagggaca cgccgttgcc gacgccgaat tcgttgatga gctcgccgat
    25381 ccacatgaga aggatcgagc ccgccgtcgc gacgaagagg ttcacgacga gctggaagtt
    25441 cgtgaggccc gggatgatgc cctggcgctc aagaagaatg agaaagccgt atgcctggat
    25501 gatcgcaagc ggcacggtga gaagcctcga gtattgggcg aacttcctgc gtcccgcctc
    25561 gccttcctcc tggtagagcg ccttgagctt cggcgacatg attgtcagaa gctgcatgat
    25621 gatggacgac gtgatgaacg gacccacgcc catcatgacg atggagagcg tggagaggcc
    25681 gccgcccgaa aagatattca aaagacccag gaattggttg ttctggagaa atgactggag
    25741 catgagcgaa ttgacgcccg gaatgggaat ggcggccaag agcctgaaaa gcgcgaagca
    25801 gccgagcacg aagaaaatgc gcttgcgaag gtcttcatcg ccgaatacca gtttgagttt
    25861 ttcggtaaat ttgctcatga atataatttt taatttccaa ttttcaatga attttcaaat
    25921 tccaatgttt caagcgtttg aagattgatt cattgtacct tgattgaaaa ttgtgaattg
    25981 caaattattt gatcggccat cagttctgcg ctcgccgtcg ctcacgcagc ctgtcggccc
    26041 cgcctcactt cagaacttta ctaagttctg agctcggaag atctcgctgt gttccggctc
    26101 aaaattactc cttattataa actaaggagt aattttaccg cctgctttct cgatcttctc
    26161 ccgggcaccc gccgacacct cgcatcctgc gatagtaaac tttttagtga gctcgcccgc
    26221 cgcgagaatc ttcaccggag aaatctttcc cgaaatcttg ggaacgacgc ctttcgccgc
    26281 gagggtcgaa ggagtgacct tgtcgccagc tttgaagacg gcttccacga cggaaagatt
    26341 caccgctgtg ggcttcactg cgacgctctt caggtagttg gtgccgcggc cgcggagctt
    26401 ggggagcttc ttaatgaggt cgcggatttc cggacggatc ttgtggcccg cacgggcctt
    26461 ctggcccttg gtgccgcggc ccgaagtctt gccccgggtt ccgccgcgtc cgacctggac
    26521 cgcacgcttc ctctgggttt tcctttgtac gttgttgaac tgcatgatga attattgttc
    26581 gcttgccgct gacgctactg caacctcctt cactttcgca agcttcgaga gtgcctttac
    26641 ggcgctgcgg gcattgttga gctggttctt cgtccttgag aaaatcttgg cgctcacttc
    26701 cttgatgcct gcaagctcca atacattgcg cacggagctt ccggcgatga ctcccttgcc
    26761 cggagcgggc ttgataagaa cgcgggcgct cgagtatttc gcctcaacct cgtgcgggat
    26821 ggaacccgac ttcgtgaggg agacgcggat catgttgcgc ttcgcgtccc gcatcgcctt
    26881 ctcgatagca agcggcgtat cgccggcctt gccgatgccg acgccgacct ggcccttgcg
    26941 gttgccggct acgagaacga cggagaagct gaacctgcgg cctccggcga cgacgcgggc
    27001 gacgcggcgg atcgagacga tcttgtgatc gaactcgggc ttcacgcgcg cttcgcggcg
    27061 gggtcctttg cggggattct tgcggaaatc tcccttgcct cccgccggcc tgcctcccct
    27121 gcctcgggga gcgcggttgt ccgccgccgg cgccgttccc atattctgct gttctttatc
    27181 gtttggcata ttcattaaaa cttgagtcct ccctcgcggg cgccttccgc ggccgccttg
    27241 atcgctcccg tgtaaatata accgccgcga tcgaagacga ccgcctcgat cttctgagcc
    27301 ttcgctttcg atgcgagatc cttgcctgcg gcgagcgatc gctcgaccag ggtcttgccc
    27361 ttgccgccgc gagtagtcgc catcgcgagc gtcgcgcctt tcgtatcatc aatcagctgg
    27421 aggacgaccg ccgtgttcga cttgaatacc gaaattctcg ggcgttccgc cgttcctttc
    27481 accttcgcgc gaatgcgctt ctggcgcctc gcccgctgtg ccattttttt gttctttagg
    27541 gtcttcatgg ttgtaagttt caagtcagat taggcggcct tcttgccctg cttgcgcctg
    27601 ataacttcgc cttcatacgc gatgcccttg cccttgtacg gctccggctt cttcaaggcg
    27661 cggacctccg ccgcgaattg gccgaggatg tccttgtcga tcgacgtgag cgtgagcgtg
    27721 ttcttctccg tcttcgccgt tactcccgcc ggaattttca tcttcaccgg atgggagaag
    27781 ccaagcgaga ggttgagtga atcgccttgc gcgtcccatt tgaagccgac gccttcgacg
    27841 acgagtttct tctcgtacgg cttattgacg ccgtcgacca tgttctggat gtgcgaggcg
    27901 tacgtgcccc agagcgcgtt cgtttcgatg ctttgcttct tcggagcgag cgtgacttcg
    27961 ccctcgccga tagtgatggc gatgtctccc ttgaacttcc ttgaaagctc gccgtgcggg
    28021 cccttcacgc tgaacacgcc gtccgattcc gaccagcggg cctcggtgtt tttggggaga
    28081 gcgataggtt ttttgccgat tcgtgacata gtggtttacc agatcttaaa caaggcttcg
    28141 ccgccgacgt tcttctcgcg agcctccttg tcggatacga tgcctgcagg cgtggtgagg
    28201 acgagaaggc cgtagccgtt cttgactggc ctcaaatcct tggcgcgagc atagacgcgc
    28261 ttcgagaatt tcgaaatgcg ttcggcgccg cgaactttcg gttcgtcatc ctcatagatg
    28321 agctcgatct cgatcgactt tgcgatcttc ttgcctttct tcgagaccga cttcacgaag
    28381 ccttctctct gaaggagctc cgcgatggac atcttcatct tcgaatacgg aacggtgaca
    28441 agcgggttgc cagccgcacc cgcattcttg atgcgggtga tcatatctgc taaggggtcg
    28501 gttaccatga tgatttctta acgcctggaa tcttgccttc gtttgccagc tccctgaagc
    28561 aaatgcggca gagatcgaag tcgcgcatat atgcgcgctt gcgaccgcac ttgaagcagc
    28621 ggcgaacgac tctcgaagcg tacttcgggg tgcgctgcga cctgacgacg actgatttct
    28681 ttgccattta gtaattaggt gtgcttttgg aatgcgcccg cggtaagccg gttcccgaag
    28741 gagcggctcg gacaggcgct taaattaaaa aagcttccaa aaagctcggg cttatactag
    28801 caaagcctcc gcaggctgtc aaacaggaag aagcttatag tttatagctg ttagttttta
    28861 gctggaaacc agtatgtgag agtcagactc tcacaggaaa tctccttcca tccgtcatgc
    28921 ccagcagagc atccaagctt caataataat tctctggatt ccctgctggg aatgacacca
    28981 ataagaaaaa accgctcaat agcggttttt tggcctactt tctactgtct acctactact
    29041 tactatcttt tttaaacggg aatccgagat attcgaggaa ggctttcgcc tcgtccttcg
    29101 aggtggcgct cgttacaacg gtaacgctca gaccgaaaag atcgcgaatg tcttcatcgg
    29161 cagtctctgg gaaaatggtg tgctcgcgga tgccgagagt gtagttgccc atttcgtcga
    29221 tgctcgtcga gggaatgccg cgaaagtcct ttgtgcgagg aagggcgaca ttcaagagcc
    29281 tgtcgaggaa atcgtacatg cgcgctcccc ggagcgtgat ctggtagccc acagggtctc
    29341 cctggcggac cttgaatgcg gcgatcgact gcttggctcc gcgctgtgaa ggctcctggc
    29401 cggtgatctt gcggaggcgg tcgacgacgg tctggttctt cgtcttgtcc ttgaaggagc
    29461 cggtgcctgc cgataccacg actttcacga ggcggggcgc ctgcatctta ttcgtatagc
    29521 cctggctttc cttgagagat tcgaatgcct tggctgtttt ttgtttggtc atttccatgg
    29581 tagtgcttta tttcgccttg gccgagccgc gcgccttgtt gagccgagtc ggtttgttat
    29641 tgacgagaag catcacgttc gagacgctta tcggggcctc cttctcgacg atctttccct
    29701 tctgatcctg acggcgggtc ttctcgtgcc tcttgcgcat attgacgccg gcgacgatta
    29761 cgcggttctc gcgcggaaag gccgtcacga cggtgcccgt cttgcctttg tcctttccgg
    29821 agatcactat gacgttgtca ttcttgcgga ttttcatatc atttcaaaaa ctagaccacc
    29881 tcgggagcga gggagatgat tttctggtag ccgacctcgc cgatctcgcg cggaatgggg
    29941 ccgaatacgc gtccggcgat cggaacctgc ttctccttgt cgacaagcac gactgcgttc
    30001 tcatcgaagc ggacatacga gccgtccttg cgcctcgtcg cgttcttcga gcggacgacg
    30061 atgccgtaga ccacagcctt cttcttgatc tgcttgcggg gctgggcctt ctgaatggag
    30121 aggaccacga tgtcgccgag gcgggcgtag cgcttcttcg aactgccgag cactttgaag
    30181 atgcggccga cagttccgcc tgagttatca gctattttta ctattgaacg gggctgaatc
    30241 atgattttga gtaatgagta gcgagtagag agtaatgaga gattgaatat tttcttttgt
    30301 tgttgatttt ctatctactg gctactatct actctgttac tatcttaaag cgcttttcct
    30361 tggagatcgg cgccgtctct tcgatggtca ccttctcgcc caccttgcgg gtattgccgg
    30421 cgtcatgagc cttgaagcgc ttcgcgcgct tcaagaattt gccgtacttc gggtgcttca
    30481 cgtacctctc gacaagcacg accacggtgt ccttcatttt gtcggacacg acgacgcctt
    30541 cgaggcgctt cttgcggcta ttctgctgtt ctgcgtcttt tttcataatt attttgctgc
    30601 ggtcttgcgg gcgttcagct cagtgagaat ctgggcgatc tccttgcgga ttgccctgcc
    30661 ctgcttcacg ttgcgggcct tgctgcccgc gatatcgaac ctgaaggtgc gaagggcttc
    30721 gcggcgctcg gtgatgaggc ccttgagttc ctgttcggtt ttgttcttga g
//
LOCUS       DASZYS010000017         8722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_22665, whole genome shotgun sequence.
ACCESSION   DASZYS010000017 DASZYS010000000
VERSION     DASZYS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22665"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VHF05_01020"
     CDS             <1..504
                     /locus_tag="VHF05_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVS79537.1"
                     /translation="YDGTLVFPRSRDGALVLLGRKAKKVGAGRWNGYGGEIESNDPNP
                     AAAAARELLEESGLSADPAKLEKVAIIYFHNRLSTGEEYTNTTHVYFVSEFEGTPIGT
                     PEMTDPTWFEIDKVPYGEMIPSDREWLGPILNGKHVIAEAWLSAFQRESLQPVQIREA
                     ASDELET"
     gene            613..768
                     /gene="rpmG"
                     /locus_tag="VHF05_01025"
     CDS             613..768
                     /gene="rpmG"
                     /locus_tag="VHF05_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HVS79538.1"
                     /translation="MSQDNLIMLVHKATGERVYTRKNKKGVERKIKLKKYSKVLRKRV
                     EFVETKK"
     gene            complement(817..888)
                     /locus_tag="VHF05_01030"
     tRNA            complement(817..888)
                     /locus_tag="VHF05_01030"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Trp,seq:cca)
     gene            complement(932..1831)
                     /gene="miaA"
                     /locus_tag="VHF05_01035"
     CDS             complement(932..1831)
                     /gene="miaA"
                     /locus_tag="VHF05_01035"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HVS79539.1"
                     /translation="MKFGKDNIIAIVGPTAAGKSDIAVRIAKAIGGEVVSADSRQIYK
                     GLDIGSGKITPRETRGVPHYMLDVADPKRTFTVAQYQKQALKEIARILKRGKIPVLVG
                     GTGLYIQAIIDGVTFPEVKPNAKLRKKLEGKSAAELAAILKKLDKKRYLEIDIHNPRR
                     LVRAIEIAKALGSVPPIKKTSDFTPLLIGIRLDDEILKKKIHARLLKRMKVGMVAEAK
                     RLHAEGLSYKRMDSLGLEYRYLAAFLQKKLSKEEMIEQLSNAIWHYAKRQITWFKKDK
                     RIKWFEPGEYEKIESKVRNFLKN"
     gene            complement(1848..2789)
                     /locus_tag="VHF05_01040"
     CDS             complement(1848..2789)
                     /locus_tag="VHF05_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79540.1"
                     /translation="MKISAFSARTMTAGALTALWLIAPGIASASFFGTPDIYTGGTVT
                     GGGSVSGGGSVFGGGAVSGGGSVFGGGSVSGGGSVFGGGSVTGGGSIFGGGSVAGGGS
                     VTGGGSVFGGGSVTGGGSIFGGGSIPGGGSVAGGGSVTGGGSIFGGGSVTGGGSIFGG
                     GSVTGGGSVFGGESVTGGGSIFGGGSVTGGGSIPAGGSITGGGSVFGGGSVTGGGSVF
                     GGGSVTGGGSIFGGGSVTGGGSIPAGGSVTGGGSVFGGGSVTGGGSIFGGGSVTGGGS
                     VMGGGSVTGGSSGVSNGGNIVFPVTGSTGTGSFFAQF"
     gene            complement(2975..3055)
                     /locus_tag="VHF05_01045"
     tRNA            complement(2975..3055)
                     /locus_tag="VHF05_01045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3020..3022),aa:Leu,seq:caa)
     gene            3161..4246
                     /locus_tag="VHF05_01050"
     CDS             3161..4246
                     /locus_tag="VHF05_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVS79541.1"
                     /translation="MSQFQNDSIFWIELDKIRPNPFQPRREFDQAALQSLADSIKQYG
                     VLQALVVTRKEEQRADGGIIAYYELIAGERRLRASKLAGLSQVPVLIKTGEENDLLKL
                     ELAIIENIQREDLNPLDRARAFLRLAEEFGFKHSEIGAKVGKSREYVTNTLRMLSLPE
                     HILEALAQGKITEGHTRPLMMLVNHPQEQDTLLKEIMYKKLTVREAELIARRIAKERV
                     RKKEYAFDPELVEIEEKLQESLGTRVQIERREQGGNVVISFFSNDDLRALLEVLDSHT
                     DKPKKVTEMLDRYIAAQQSSQSDQPAESSPASSDISVSTASAAVVTVSTEPEIAPEES
                     GPIDDRTREEIKEQEDEDIYSIKNFSI"
     gene            complement(4243..4467)
                     /locus_tag="VHF05_01055"
     CDS             complement(4243..4467)
                     /locus_tag="VHF05_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79542.1"
                     /translation="MTLETENFQIWMQFEENTSSATKVYEKYSAGACGVSRDYWKRRK
                     AGRKEAGSQKPPSRAVYCEFDKEMRQICKF"
     gene            complement(4520..6034)
                     /locus_tag="VHF05_01060"
     CDS             complement(4520..6034)
                     /locus_tag="VHF05_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HVS79543.1"
                     /translation="MEVSEIIELIKSPSPEDVALIQKAYGFAKEAHKDQKRFSGDPYF
                     SHLFETAKILAELGMSPTTIAAGLLHDSVEDVGVTRETLQAEFGDEILFLVEGVTKLG
                     QIRYHGSNWHTESLRKLFVAMSQDIRVLMVKLADRLHNMRTLSSVPQEKQKRIAAETL
                     EVYAPIADRLGIGRLHRELEDLAFPFVFPEHYAKTRELVARRQEEMNADLEKFHTSLE
                     KVFSDAGFPQAKTSYRVKGLYSLYKKLARNNMDIEKVYDLMALRVIVDTVADCYQALG
                     IVHANWRPLPGRIKDFIAFPKTNGYKSLHTTVFTGDGGIVEVQIRTADMHREAEYGIA
                     SHLSYKQGAAKGSAAHPALEWVKGLIPSRKVGGLGAASAHLSYSDVPEWIKELVEYQK
                     SQGDQEFEEGLKEDFFRERIFVFTPVGDVVDLPIDSTPVDFAYAVHSDIGNHMSGAKV
                     NGKLVSLDTALRNADIVEIQTKKNAKPNRKWIDSAKTSMAKKHIRQALEKIERQ"
     gene            complement(6735..>8722)
                     /gene="topA"
                     /locus_tag="VHF05_01065"
     CDS             complement(6735..>8722)
                     /gene="topA"
                     /locus_tag="VHF05_01065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HVS79544.1"
                     /translation="SVISEIKNLAKNAGEVLLATDPDREGEAIAWHIAEAAGLKHPKR
                     VVFHEITKDAIIEALKHPRGIDMDLKEAQEARRVLDRLVGYDLSGLIWKKVRYGLSAG
                     RVQSPALRILMEREREIRAFISHKFWVITADVKNKKNDAFILECTVEPTEKKETERIL
                     EHSKKGTWIVKDVAETEAKRTPRPPFITSTLQQAASSRLGFSPSNTMRIAQKLYEAGL
                     ITYMRTDSTNLSKVAVAQAAKVVEKKYGKELVAVRTFAKKSKNAQEAHEAIRPTDLSR
                     EMAGFAPDQQKLYKLIWARTLASQMADAKILRTKMIVEVDKGSVPDFAINGARVLQPG
                     WLLADPAARGEDVELPKVDVGEELSLVEMHDEEKETQPPSRYTEAGLVKELEKRGIGR
                     PSTYASIIKTLQDRGYVTKEGRTLIPTDTGDVVSSFLEKYFEKYVSDNFTAFMEDKLD
                     DISEGKTTYEKVLKEFYAEFLKDVKSKEKIDKITNLGAADPSMKCPVCGSGMIVKLGK
                     SGKFLSCERFPDCTGARTIEGKEMTGPKDTGEICPRCGEGTLVEREGRFGAFIACSRY
                     PKCKFIKEDEEKKKAAMTGVKCPVCGTGEMMEKRGRFGTFYACSNYPECKNAIKAKPT
                     GAICPLCNSLMMEGTKTIPERCSNKACPNHNPHKIAK"
BASE COUNT         1834 a         2398 c         2448 g         2042 t
ORIGIN      
        1 tacgacggga cgctcgtatt tccgcgatca cgagatggcg cattggtcct gcttgggcgc
       61 aaagccaaga aggtcggcgc tggccgctgg aacggctatg ggggcgagat cgaatcgaat
      121 gatccaaatc ccgctgccgc cgcggcacgc gaacttcttg aggaatcggg actgagtgcc
      181 gatccggcaa aattggagaa agtggcgatt atttattttc acaatcgctt aagtactggc
      241 gaggaataca caaatacgac gcacgtctat ttcgtaagcg aattcgaagg cacgcccatc
      301 ggcacgccgg aaatgacgga tccgacatgg tttgagatcg acaaagttcc gtatggagag
      361 atgattccat ccgacaggga atggctgggt cctattctta acggcaaaca tgtcattgcc
      421 gaggcgtggc tcagcgcctt tcaaagggag tcgctccagc cggtgcaaat ccgagaagcc
      481 gcatcggacg agctggaaac ctagaagagc cgtatatggc tcttcttttg catataggct
      541 aggaatcttg ggcgaatact tgctttttcc tacggtttaa agtattcttt tccctgttaa
      601 tttcacacgc atatgtccca ggataacctc atcatgctcg tccataaggc caccggagag
      661 cgcgtttaca cccgcaagaa caagaagggc gtcgagcgca agatcaagct caagaaatac
      721 agcaaggtcc tccgcaagag ggtcgagttc gtcgagacga agaaataaga caaaccgccg
      781 aaaggcggtc ttgttttaca taagaagttg tctcagcggg cccacgagga ctcgaacctc
      841 gaacctcggt tttggagacc gatgttttac cattgaaact ataggcccaa tcgcccctga
      901 tactaccata cttccaaatt ccctgtatag atcagttttt gaggaaattg cgcaccttgc
      961 tttcaatttt ttcgtactct ccaggttcaa accatttaat ccttttgtcc ttcttgaacc
     1021 acgttatctg ccgtttagca tagtgccaaa ttgcattcga caattgctca atcatttcct
     1081 cctttgagag ttttttctgg agaaacgcag cgaggtagcg atactcgagg ccgagagaat
     1141 ccatgcgctt gtacgagagt ccttccgcat ggagacgctt cgcttcggcg accattccta
     1201 ccttcatccg cttcagaagt cgggcatgga ttttcttctt gagaatctcg tcatcgagcc
     1261 gaatgccgat gagaagagga gtgaaatcgg aagtcttttt gatgggtggt accgagccga
     1321 gcgccttggc gatctcgatc gcgcgtacga gcctgcgggg gttatggata tcgatttcca
     1381 agtagcgttt cttgtcgagc tttttcaata ttgccgcgag ctcggccgcg ctttttccct
     1441 cgagcttttt gcgaagcttc gcgttcggct ttacctcggg aaacgtaaca ccatcgatga
     1501 tcgcttggat gtagaggccg gtgccgccca cgaggacggg aattttgccg cgcttcagaa
     1561 tacgcgcaat ctctttaaga gcctgtttct ggtactgcgc gacggtaaaa gtgcgctttg
     1621 gatcggcgac atcgagcata taatgcggaa caccccttgt ttcgcgaggg gtgatttttc
     1681 cgctgccgat gtcgagtcct ttgtagatct ggcgcgaatc agctgagacg acttcgcctc
     1741 cgatcgcttt cgcgatacgg acggcgatgt cgcttttgcc tgccgctgtg gggccgacaa
     1801 tggcaatgat gttatctttg ccgaatttca agcttgagat tcaaatgtta gaactgggcg
     1861 aagaatgatc ccgttccggt ggagccggtg acagggaaga cgatattgcc gccgttcgag
     1921 acgccgctcg atccgccggt aacgctaccg ccacccatga cggaacctcc tccggtgact
     1981 gatcctcctc cgaagatgct accgcctccg gtaacgctac cgccgccgaa gactgaaccg
     2041 ccgccagtta ctgaaccgcc ggccggaatc gatcctccac ccgtaacgct tcctccaccg
     2101 aagatggagc cgccacccgt gacagaaccg ccgccgaata cggagcctcc gccggtgacg
     2161 ctaccgccgc cgaatacgga gccgcctccg gttatggagc cgcctgcggg aatagaacct
     2221 ccgccggtta ccgatcctcc tccgaagatc gaaccgcctc cggtaacgct ttcgccacca
     2281 aagactgatc cgcctcccgt gacgctacca ccgccgaaga tcgagccccc gccagtaacg
     2341 gaacctcctc caaaaataga tccgccgccg gtcaccgatc ctcctccagc gactgaaccg
     2401 ccgccaggaa tgctgccgcc gccgaaaata gaacctccgc cggttaccga accgcctccg
     2461 aagactgaac cgccgccagt aacactaccg cctcctgcta cgctgcctcc tccgaagatg
     2521 ctgccgcctc cggtaacaga accaccgccg aatacagagc cgccgccact gacagaaccg
     2581 cctccgaata cggatcctcc gccggaaact gcgcctccgc cgaagacaga accgcctcca
     2641 gaaacactgc cgccgccggt tactgtcccg ccagtgtaga tgtcgggcgt gccgaagaac
     2701 gaagcggatg caattccagg agcgatgagc caaagagccg tgagggctcc ggcagtcatg
     2761 gtgcgagcgg aaaatgctga aatcttcata tatttatatg agtaatgcta ttaaaacaaa
     2821 gcttgaaata aggcttcggt tagcagtatt tgctaacgaa agaccctacc ataagttaat
     2881 ttaaaagtca agtacatctg tgatcctcct atatataaag acgttttgga aatgaagagg
     2941 ggataaagaa aacgcccttt cgggcgttca tcagtgccgg aggagggact tgaaccctca
     3001 caccttgcgg tacacgattt tgagtcgtgc gtgtctgcca ttccaccact ccggcaagta
     3061 tgaagacaag ctaagtctac tgaaaatggg gctggcgcgc aattgtgtct gtcgcgcatg
     3121 agcgtgatat aatcgagagg taagttaccc cacgaagagt atgtcacagt ttcaaaacga
     3181 ctcaattttc tggatcgagc tcgacaagat ccggccgaat ccgtttcagc cgcggcgcga
     3241 gttcgaccag gccgcgcttc agagcctggc cgattcgatc aagcaatatg gcgtcctgca
     3301 ggcgcttgtc gtgacgcgca aggaagagca gcgcgccgac ggcggcatca tagcttatta
     3361 tgagctcatt gccggagagc gcaggctccg cgcctcgaag ctcgccggac tctcccaggt
     3421 gccggtcctc atcaagacgg gcgaggagaa cgatcttctg aagctcgagc ttgcgatcat
     3481 cgaaaacatt cagcgcgagg acttgaatcc tctcgatcgc gcccgcgcct ttctccgcct
     3541 cgcggaggaa ttcggcttca agcactccga aatcggcgcc aaagtcggga agagccgcga
     3601 gtacgtcacc aacacgctca ggatgctttc gctccccgag cacatcctcg aagcgctcgc
     3661 gcaaggcaag atcaccgaag gccacacgcg cccgctcatg atgctcgtca atcatccgca
     3721 ggaacaggat acgctcttga aggaaatcat gtacaagaag cttacggtgc gcgaagcgga
     3781 gctcatcgcc cgccgcatcg ccaaggagcg ggttcgcaag aaggaatacg cttttgatcc
     3841 ggaactcgtc gagatcgaag agaagcttca ggaatccttg ggcacccgcg ttcagatcga
     3901 gcgcagggaa cagggcggca acgtggtcat ctcattcttt tccaatgacg atcttcgcgc
     3961 ccttctcgaa gtgctcgatt cccataccga caagcccaag aaagtgaccg aaatgctcga
     4021 tcgctacatt gccgcgcagc aatcgtcgca atccgatcag cctgccgaaa gctcgcctgc
     4081 atcgtccgat atttccgtga gcactgcgtc tgccgcagtc gttaccgttt ccaccgaacc
     4141 cgagatcgct ccggaagaga gcggcccgat cgacgatagg acaagagagg aaatcaagga
     4201 gcaggaggac gaagatatat actccatcaa gaatttcagt atttagaact tacatatttg
     4261 gcgcatttct ttgtcgaact cacaataaac cgcccgagag ggcggttttt gcgagcccgc
     4321 ctcctttcgt ccggccttgc gccgcttcca gtagtcgcgg ctcactccgc acgctcccgc
     4381 ggagtatttt tcatacacct tggtcgcgct gctcgtattc tcctcgaact gcatccaaat
     4441 ctgaaagttc tctgtttcca gtgtcattcc cagcagggaa tccagtggct tttattcggt
     4501 tctctttttt caatgcgttt cactgtctct ctatcttctc caacgcctgc ctgatatgct
     4561 tctttgccat cgaagttttg gccgagtcga tccacttgcg gttcggcttg gcatttttct
     4621 tcgtctggat ctcgacgata tcggcgttcc gaagcgcggt gtcgagagag acaagcttgc
     4681 cgttcacctt ggcgcccgac atgtggttgc cgatatccga gtgcaccgca taggcgaagt
     4741 cgaccggcgt cgagtcaatt gggaggtcga cgacgtcgcc cacgggcgtg aagacgaaga
     4801 tgcgttcgcg gaaaaaatcc tccttgagcc cttcctcgaa ttcctggtca ccctgtgatt
     4861 tctgatattc gacgagctcc ttgatccact caggcacatc agagtagctc aggtgtgcgg
     4921 aagcggctcc gaggccgccg actttccgcg aaggaataag ccccttgacc cattcgagcg
     4981 cgggatgcgc ggcactgccc ttggccgcac cctgcttgta tgaaagatga gaggcgattc
     5041 cgtactccgc ttcgcggtgc atatcggcgg tgcggatctg gacttcgaca atcccgccgt
     5101 cgccggtgaa gacagtcgtg tggaggctct tgtagccgtt ggttttcgga aacgcgatga
     5161 agtccttgat gcggccggga agcgggcgcc agttggcgtg gacgatgccg agcgcctggt
     5221 agcaatccgc aacggtgtcg acgatgacgc gaagcgccat gaggtcgtag accttttcga
     5281 tgtccatatt gttgcgggcg agcttcttgt agaggctgta gaggcctttg acgcgatagc
     5341 tggtcttggc ctggggaaaa ccggcgtcac taaaaacttt ctcgagcgag gtgtggaatt
     5401 tctcgagatc ggcgttcatt tcctcttggc ggcgggcgac gagttcgcgc gttttcgcat
     5461 agtgctccgg aaatacgaaa gggaacgcga gatcttcgag ttcgcggtgc aggcggccga
     5521 tgccaaggcg gtcggcgatg ggagcgtaga cttcgagcgt ctcggccgcg atgcgcttct
     5581 gcttctcttg cgggacgctc gagagcgtgc gcatgttgtg gagcctgtcg gcgagcttca
     5641 ccatgagcac gcggatgtcc tgcgacatcg ccacgaagag cttgcggagg ctttcggtat
     5701 gccagttcga tccgtgatag cggatctggc cgagcttcgt gacgccttcg acgaggaaga
     5761 gaatctcgtc gccgaattcg gcctggagag tttcgcgggt tacgccgacg tcctcgacgg
     5821 agtcgtggag aaggcccgcg gcaatcgttg tggggctcat gccgagctcg gcgaggatct
     5881 tcgcggtctc gaagaggtgc gagaaatacg ggtcgccgga aaagcgtttc tgatccttgt
     5941 gcgcctcctt cgcgaagccg tacgcttttt gaatgagcgc gacatcttcg ggagaaggcg
     6001 actttataag ctcgatgatt tcgctgacct ccatcccgtt agaggcagat cacgctcact
     6061 gtgtggtctg ctgagttatt cgtttttctt gtaaataatt cgaccatctg tgtgatattc
     6121 atttcgagtg atcgcggcct ctaacgggat ccatggcttc attctatgga aaggaaatgt
     6181 gttcggcaaa ttggcaggag gcgaaatgtg aaaacggccg cgcgcgaagc gtggccgttt
     6241 ggttatgggt tactgcatgg aactacacat aattatatga gtcccgaaaa tttttgcggc
     6301 tggcgacgga gttattttcc tccgctatcg gtccagccaa agaaagtact attatttttc
     6361 attcatctcc tccagagatc tgaactaact agtgagaaag gattgagagc tcagagaact
     6421 gaaggacctg ttccagaaag gagatttacc cgaaggcgtc tcttggtttc ctgaattatt
     6481 aaatcaaatc tacagaattt cgcaagaaag ttcatgaaga gaggaggggg agatggtttt
     6541 tggtcaggaa taattttctt gccggtcgca agaaaatttt ccgcgatcta ctgaatggcc
     6601 ttgggtcaca aatcaaaaaa gctccgtcga gcttttttgt tcgcgaacct cgcctcggta
     6661 tttcggactg ctgtccgaaa tatacctgcg gctcacaaag actcattgcg tatgcagcga
     6721 aggctgctac cgtactactt cgcgatttta tgcggattat ggttcgggca agccttgtta
     6781 ctacaccttt cagggatagt ctttgtgcct tccatcatga gtgagttgca gagggggcag
     6841 atggcacccg tgggttttgc cttgattgca ttcttgcatt cgggatagtt ggagcaggcg
     6901 tagaaggtgc cgaaacggcc gcgcttctcc atcatttcgc cggtgccgca aacggggcat
     6961 tttacgccgg tcatcgccgc ctttttcttc tcctcgtctt ccttgatgaa tttgcacttc
     7021 ggatatcttg agcatgcaat gaaagcgccg aagcggcctt cacgctccac gagcgtccct
     7081 tcgccgcagc gggggcagat ttcgccggtg tccttggggc cggtcatctc cttgccttcg
     7141 atcgtccgcg cgccggtgca gtcggggaag cgctcgcacg agaggaattt gccgctcttg
     7201 ccgagcttca cgatcatgcc gcttccgcag acgggacatt tcatcgatgg gtcggcggca
     7261 cccagattcg tgatcttgtc gatcttctcc ttcgacttca cgtccttgag gaattctgca
     7321 tagaactcct tgaggacttt ttcgtacgtg gtttttcctt cggaaatgtc gtcgagcttg
     7381 tcttccatga aagcggtgaa attgtcgctc acgtatttct cgaaatattt ttcgaggaac
     7441 gaggaaacga cgtcgccggt atctgtgggg atgagggttc ggccttcctt cgtcacgtag
     7501 ccgcgatcct gaagcgtctt aataatggag gcataggtgg acggccggcc gatgccgcgc
     7561 ttctcgagct ctttgacgag gcctgcttcg gtgtagcgcg aaggcggttg cgtttctttc
     7621 tcctcgtcgt gcatttcgac gagcgaaagc tcttcgccga catcaacctt gggaagctcg
     7681 acatcctcgc cgcgcgctgc cgggtcggca agcagccagc cgggctggag gacccgcgcg
     7741 ccgtttatcg cgaagtcagg caccgagccc ttgtcgactt cgacgatcat tttcgtgcgg
     7801 aggatcttgg cgtccgccat ttggcttgcg agggtcctcg cccagatgag cttgtagagc
     7861 ttctgctggt cgggagcaaa gcctgccatt tcgcgggaga ggtcggtcgg gcggattgcc
     7921 tcgtgcgctt cctgcgcgtt cttgcttttc ttggcgaagg tgcggacggc gacgagctcc
     7981 ttgccgtatt tcttctcgac gactttggcg gcttgcgcga cggcgacctt cgagagattg
     8041 gtgctgtcgg tgcgcatgta ggtgatgagg cccgcttcgt agagcttttg ggcgatgcgc
     8101 atcgtgttgg acggcgagaa gccaaggcgc gagctcgcgg cctgctggag ggtcgaggtg
     8161 atgaacggcg ggcggggagt gcgcttggct tcggtttcgg cgacgtcttt cacgatccac
     8221 gtgcctttct tgctgtgctc gagtatgcgc tcggtttcct tcttctcagt cggttcgacg
     8281 gtgcattcga gaatgaaggc gtcgttcttc ttatttttaa catcggcggt gatgacccag
     8341 aacttatgcg aaatgaacgc gcggatctcg cgctcgcgct ccatgagtat ccgcagggcg
     8401 ggggactgga cgcggcctgc cgagaggccg taccgcacct tcttccagat gaggccggaa
     8461 aggtcgtagc cgacgaggcg gtcgaggact cgccgcgctt cctgggcttc cttgagatcc
     8521 atgtcgatgc cgcgaggatg cttcagggcc tcgatgatcg cgtccttggt gatctcgtgg
     8581 aagacgacgc gcttcgggtg cttcaagcct gccgcttccg cgatgtgcca tgcgatcgct
     8641 tcgccttcgc ggtcggggtc ggtagcgaga agaacctcgc ccgcattttt agcgaggttt
     8701 ttgatctcgg agatgacgga tt
//
LOCUS       DASZYS010000018        15042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_23377, whole genome shotgun sequence.
ACCESSION   DASZYS010000018 DASZYS010000000
VERSION     DASZYS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15042
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23377"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VHF05_01070"
     CDS             <1..1611
                     /locus_tag="VHF05_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVS79545.1"
                     /translation="ISNFEFKKGVRVTEQTLVRTKPSINKWLVAIAVMSSAIIEVLDT
                     SVVNVSLPHIAGSLSATTDEATWVLTSYLVANAIVLPLSGWLANYFGRKRLLMSVVCG
                     FTVSSLLCGMAPNLPSLVFFRIMQGVTGGGLQPLSQAVLLEEFPRKEHGKAMAVWAMG
                     IVVAPILGPTLGGWLTDNYSWRWVFYINLPIGIISLVLIGLYVWDPPYISRGKLRFDA
                     WGLGMLVVGMGAFQIMLDKGQEEDWFASKFIVVLCVVAIVLLTAFVVRQLKVENPLVK
                     FRLMSDRNFACGISLVSLMLFVLYGSLVLLPLFMQELLGWTAVTTGFWTGPRGIAIAV
                     AMPVVGKLLGKGWDGRLMVAGGFAAASISYLAGYSHMNLNSGAWDIVWPQLIQGVGVG
                     FIFVPLTVLTMSRIQKQDMGYASSLYSIMRNIGSSIGIPFVTTLIARRSQFHQVRLGE
                     HVTAYDPRATQYLSTTAHFFANAGSDAVAAKMQGIEVVYRSMLQQASLMSFLDAFKIM
                     GFVLIGVIPLIFIMKKASKKPQKMEMME"
     gene            complement(1685..2092)
                     /locus_tag="VHF05_01075"
     CDS             complement(1685..2092)
                     /locus_tag="VHF05_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVS79546.1"
                     /translation="MDPVVHFEMPFDDRDRMVGFYAKAFGWQANLLGEDMGNYAVMMT
                     GEDDPATRRPKEPGRINGGFFKRNPKNAYPSVTIAVKDIKAAMKKVEEAGGHAIGGHF
                     ENGEPDMIPGVGLYASCIDTEGNRISLLQPMGM"
     gene            2295..2828
                     /locus_tag="VHF05_01080"
     CDS             2295..2828
                     /locus_tag="VHF05_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79547.1"
                     /translation="MKRSTKITTGLAAAALTIGLGFSGAGLAHAQTASTTPESRLASL
                     AQAIASKFNLNESDVESVIKDHRAEMQAEREERYKSALDKAVQNSKLTQDQEDKILAK
                     MEELKSERESLQGKTPEEIRSALKTEAASLKQWAADNNIPTEYLRPGFFGGPGRFMGP
                     LGPKDASSTDATSSSQN"
     gene            complement(2895..3557)
                     /locus_tag="VHF05_01085"
     CDS             complement(2895..3557)
                     /locus_tag="VHF05_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79548.1"
                     /translation="MKKTFIITAAVILAIFIIALAIWLPNRNDSSAADRTEQSTAPAT
                     PPVATYGNTAYGYELDFPPELETRMYSQDDVVFGHKEGDGIDGVAEARVLKTGDAEGG
                     ELENAVIGEMANLCAADGPNESLSCSGADQAGAQAFISDSGLRGFIFYLSGEIKNTSA
                     GSSTPIEKGPYVAFPLTDGATLVIHPPLNKNAKETDASVITQVAKSVRIKDAAISSPS
                     GE"
     gene            complement(3576..3989)
                     /locus_tag="VHF05_01090"
     CDS             complement(3576..3989)
                     /locus_tag="VHF05_01090"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HVS79549.1"
                     /translation="MTGINFLAVLIAAVASMIVGSIWYGPLFGKRYIREMGWENHTPE
                     ERERMKKSMTKSYVIQFVASFVMFFVLDWYINTGINTGLTAGIGNAFGAWIGFIVPIK
                     LGDVLWGGKKSLFWLSIGNTLVTLLVGGAILSFFA"
     gene            complement(4054..5562)
                     /locus_tag="VHF05_01095"
     CDS             complement(4054..5562)
                     /locus_tag="VHF05_01095"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVS79550.1"
                     /translation="MFKHFWGSLNPMASLGEKNHSIYIPLLGSAAAYLLAEFIGNLIL
                     HRPDNMGPEVVVLTFLLVLYFSFRHAIRGGLTASAVSIVYFTYFIYSRTASYSVKVAA
                     TENVILLTSLYLLITFTVGILRKRLDDLFGKEQQARAAAEHDRRRMEALLKQLPVGVL
                     LAQSPTGEIISRNNMALELLGPAVATPVSSIEEYVYPSATIGGKKIEPREWPLARALT
                     TGEIVTGEEIVITGPNGDSTTLFFNASPVYDKKGNITAAVATFYDITKQKELERLKDD
                     FVAMISHELKTPLTSAKIYAQSLLKEMTNSQEQGAFMEKMNDQLDRLALLVNTMLDTT
                     RLEQGKLELQKERFVLGDIAREIVGELRDTTGRKISLDWQADKPLTSNKDRIAQIFTN
                     LLTNALKFSPKDSEVIVRSREKDGMIVVSVQDFGIGLSQEALDHIFERFYQSEEHKTY
                     PGLGLGLYISSQIIRKLGGEMRVESEKGKGATFSFSLPLEDKPSLAGKEPAM"
     gene            5724..5906
                     /locus_tag="VHF05_01100"
     CDS             5724..5906
                     /locus_tag="VHF05_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79551.1"
                     /translation="MSLQEMHDEHAQYWRDKDGIKFGLASMRWHGWGSPVGLGLFLLS
                     IGGFLLLLHFAGLIGG"
     gene            complement(5978..6538)
                     /locus_tag="VHF05_01105"
     CDS             complement(5978..6538)
                     /locus_tag="VHF05_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79552.1"
                     /translation="MKKIFITILVLVIAGIGIYFYMHKNGSAKNPGSAGQTSAASFDG
                     RNSTFAIDGNPVTLADGISEVPIPDSSSKVTTRYFGNEAAGDLDGDGTSDMAFIVTQD
                     SGGSGLFYYAVAALHTSSGWKTTNAYLIGDRIAPQSTVIPANSLELEVNYADRDPNEP
                     MSTAPSQAKTLTLKVAPGNVLEPSSD"
     gene            6638..6949
                     /locus_tag="VHF05_01110"
     CDS             6638..6949
                     /locus_tag="VHF05_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005260917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HVS79553.1"
                     /translation="MAKTISSGTVHALPADLRKALLSDKAALAKWEDITPLARNEWIC
                     WTISVKMAETRKEHVERVVSELKEGMRRPCCWIGCIHRPEKAISPSVQWALGKRTKRK
                     V"
     gene            6965..7372
                     /locus_tag="VHF05_01115"
     CDS             6965..7372
                     /locus_tag="VHF05_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVS79554.1"
                     /translation="MKEDAQFYIGQKAFIRRGDEILILGDPHEGLDYPGGKIQEGEID
                     LAESLKREVREETGLEIEVGTPFATWTNTFPPHHRLAGKRVYLVGYRCNYVSGDVKLS
                     DEHDKFRWVNKENYKEADDGTSYFEILDKYFEE"
     gene            complement(7428..7844)
                     /locus_tag="VHF05_01120"
     CDS             complement(7428..7844)
                     /locus_tag="VHF05_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79555.1"
                     /translation="MHTRTLRELAKFASGLIMGDILCGLGFYFGGYLPITFLGISFDA
                     QTVVAWLIFDVLLFAFLVHYGWNTKDRSHTSREKKFLAIAGVLFTAVALAHLSRIVFG
                     WNLTLGSWEVPYWLNGLGTIATAFLAYASFHFASRK"
     gene            8006..8482
                     /locus_tag="VHF05_01125"
     CDS             8006..8482
                     /locus_tag="VHF05_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVS79556.1"
                     /translation="MKRNRQKYSRGFTLIELLVVIAIIGILSAVVLASLNSARAKGAD
                     AAVKSDLQSIRNEAEIFYDGVGNNTYGPALITTICPTTGSTMFVQDATIANAIKQATA
                     ATGKTVSDAFCGTDGSNQYVVVVPLKTSGYWCIDSSGQSKSETDEQVAGGSGYVCP"
     gene            8676..10937
                     /locus_tag="VHF05_01130"
     CDS             8676..10937
                     /locus_tag="VHF05_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVS79557.1"
                     /translation="MSKPTHQGEDRKKRIHHKMSPRKRQWGAEKRTDPLSHLHETPSD
                     FAVTYSRIAIVISIVFWLMYLTSVIIRQMIDGPKDYNFTAQTIGYTAVMFFLTLSALV
                     YLITRQGSLQRFKKHIRTPRRVLEEFFSSNQPSITVLIPSYNEEVSVIRKTMLSAALQ
                     EFSNIRVVLLLDDDPFKVKPEDRERIDKTHALPAEIETLLSEPLSRAQALRAEILNAE
                     RKGGEHVLKIAAAYHSAAEWLEATAEKEIIEDHVDIFFADQVLRSLAGDLRAAAQDMT
                     AALQRGVEFSNERLEQLCNRLVWIFNAKLGFFERKRYISLSREKNKAMNLNSYIGLMG
                     GAYRKLETEDGTVLMPAEDEASADLVVKDSDFILTLDADSILLREYCQRLVYFLYQPD
                     AADVAVVQTPYSSFRGASTRLERLAGATTDIQHIIHQGMTHYGATFWVGANAVIRKRA
                     LEDIVEREFIGGFEIKRYVQDHTVIEDTESSVDLALHGWRLMNYPERLSYSATPPDFG
                     SLAVQRERWANGGLLIFPKLLKLRRERKHAGRPLHMLEFLIRMCYMTSIAWSSVGLLF
                     ILAYPYDGRLLSPLVLLAAAPYFIAMAMDLKACRYRYSDIFLIYGFNLLLLPVNLVGT
                     FKSLQQAVTTKKIPFARTPKVKNRTATAWQYLLAPLFIVGFSLFTMWRNIEGENWGNA
                     AFAGFNAFTASWAIVAYIGIKNMIVDLWLAYRDWLYVEVPVKKASKKKRKASAPEPVE
                     LAPEPIVMAGQNN"
     gene            10943..12532
                     /locus_tag="VHF05_01135"
     CDS             10943..12532
                     /locus_tag="VHF05_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HVS79558.1"
                     /translation="METKDTKMQNGTVRRLSFVRLAISVLAVIAIIGSAATSFADWQQ
                     SAELGKTGFAPWFAAYVDVTSTPTYPFEQYGSTAELNNAVLAFIVSSPAQACTPTWGG
                     QYTLDSAGEMLDLDRRIARLEQQGGRVAISFGGLLNSELAVKCTDAAQLAKAYKSVID
                     RYSVSTIDLDLEGDGLNSADAVKRRAAVIAELQKDYRSEGKALAVWVTLPVTPQGLSK
                     EGTDAVAEMLSSGVDLAGVNAMTMDYGQALNANDSMAAASEKALLETHRQLGILYSEA
                     GINLNSDSLWRKIGATPMIGQNDIADEVFTPDDAVELNKYAFRVGIGRMSMWSANRDV
                     ACGKNYVNVKVVSDVCSGVDAGPFAFVHALGQGFVGDIIQNATIVTVADPDSNKPIVD
                     DPLTSPYPVWEQSAVYLKDAKVVWHGNVYQAKWWTKNDVPDNPVLQSADTPWQLIGPV
                     LPGDKPVKLLTLPAGTYPDWRGEAIYNEGDRVIFNGMPYEAKWWNQGQSPAAAAVDPD
                     NSPWIILTQEQIEAIREARGL"
     gene            complement(12613..13161)
                     /locus_tag="VHF05_01140"
     CDS             complement(12613..13161)
                     /locus_tag="VHF05_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HVS79559.1"
                     /translation="MRKLIMWNVITLDGYFEGEKAWDLDFHGLVWGPEMEQFVAEQLK
                     TIDTIVFGEKTYKGMADYWSKAKGETAEYMNKLPKIVCSTMLATADWNNTTIVRDAVA
                     ELSKMKEEGDGNMFVFGSGKLSESLMKADLFDEYRLCIAPIFLGKGGRLFNEGLPYQK
                     LKLLEARPLQTGGVILRYARNS"
     gene            complement(13242..13694)
                     /locus_tag="VHF05_01145"
     CDS             complement(13242..13694)
                     /locus_tag="VHF05_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVS79560.1"
                     /translation="MKSIRHSIEIDAPKEKIWQVLWDDKTFRDWANIIDQGTYMTGTL
                     QEGAEVNFIGNSDGKVRYGVSSKVEKLIPLKLISFRKVEDIKVADDGSIEKRDDQWTG
                     GVESYELEEHDGQVTLSIIEDVPDELVEYFENTLPKVLERIKALAEAE"
     gene            complement(13936..14193)
                     /locus_tag="VHF05_01150"
     CDS             complement(13936..14193)
                     /locus_tag="VHF05_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79561.1"
                     /translation="MAKWIVDCGLFIFGYLVREFWNKIVELNKDRGAPLEDQIWVASI
                     FHLSINVMEPVHGKSGQGILGMESKKKGVLRGGKTKSPKIQ"
     gene            complement(14386..14679)
                     /locus_tag="VHF05_01155"
     CDS             complement(14386..14679)
                     /locus_tag="VHF05_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79562.1"
                     /translation="MRKYLTDHAGRLRREMSAYPHWFDPNRYGDDEQYLTKLDEAKAR
                     MMMCGQVFYGWSMYEVDGVFLKSIKRNGTIITDEERTQVIRLMFKFEDKRAEA"
BASE COUNT         3441 a         3943 c         4031 g         3627 t
ORIGIN      
        1 atttccaatt tcgaattcaa gaaaggagta cgcgtgacag aacaaactct cgttcgcacc
       61 aagccttcaa taaataaatg gcttgttgcg attgcagtca tgtcgagcgc cattatcgag
      121 gtgctcgaca cgtcggttgt caatgttagc ctcccgcata tcgcgggcag tctctctgcg
      181 acgactgatg aagcgacatg ggttcttaca tcgtatttgg tcgcgaacgc aatcgtcctg
      241 ccgctctccg gctggctggc gaactatttt ggccgcaaac gactgctcat gtcggtcgta
      301 tgcggcttca cagtctcgag ccttctctgc ggcatggcgc caaatcttcc gtcgcttgtt
      361 ttctttcgga ttatgcaggg tgttacgggc ggaggtctcc agcctctctc gcaagccgtg
      421 cttctcgagg aatttccgcg gaaagagcac ggcaaagcta tggctgtatg ggcaatggga
      481 attgtcgtgg cgcctatact cggccccacg cttggcggct ggcttacgga taattattcc
      541 tggcggtggg ttttctatat caacttgccg atcggaatca taagccttgt tctcatcgga
      601 ttgtatgttt gggatccgcc atatatttcc cgcggcaaac tcaggttcga tgcatggggg
      661 ctcggcatgc tggtcgtcgg catgggcgca ttccagataa tgctcgacaa gggccaggaa
      721 gaagactggt tcgcgtcgaa attcatcgtt gttctctgcg tagtcgcaat cgttttgctc
      781 acggcattcg ttgtgcgtca gctcaaagtg gagaacccgc ttgttaaatt ccgactcatg
      841 agtgaccgga atttcgcctg cggcatttca ctggttagtc tcatgctctt tgttctctat
      901 ggtagcctcg tgcttctgcc gctctttatg caggagctcc tcggatggac ggcggttact
      961 accggatttt ggacagggcc gcgaggtatt gccatcgcgg ttgcaatgcc agttgtcgga
     1021 aagcttctcg gaaagggatg ggatggcagg cttatggttg cgggcggctt cgctgcagca
     1081 agcatctcat atttagctgg ctattcgcat atgaatctca attctggcgc atgggatatt
     1141 gtctggccgc agcttattca aggcgtcggg gtcggtttca tcttcgtgcc gctcacagtg
     1201 cttacaatga gccgtattca aaagcaggat atgggctatg cctcgagctt atatagcatt
     1261 atgcgcaata tcggctcgag catcggcatt ccgttcgtaa cgacacttat cgctcggcga
     1321 tcgcagttcc atcaagtgcg gctcggagaa catgttacag catatgatcc gagagcaacg
     1381 cagtatcttt cgacgacggc gcatttcttc gcaaatgcgg ggagcgatgc tgttgctgcc
     1441 aagatgcagg gcatcgaggt tgtctatcgt tcgatgctcc aacaggcttc tctcatgagc
     1501 tttcttgatg cattcaagat catgggattc gttcttatcg gcgtcattcc gctcatcttc
     1561 atcatgaaaa aggcttccaa gaagcctcag aagatggaga tgatggaatg aaaattcctt
     1621 aagtacaaat actccggcac tattcagtgc cggagtattt ttatatgaga aagcggaagg
     1681 tgcgctacat tcccatcggc tggagaaggc ttatgcggtt gccttcggtg tcgatgcagg
     1741 aagcgtaaag gccgacgccg gggatcatat ccggctcgcc gttttcgaaa tggccgccta
     1801 tcgcatgtcc gccggcttcc tcgaccttct tcatggccgc cttgatgtcc ttcaccgcga
     1861 tcgtaacgga agggtatgcg tttttcggat tcctcttgaa gaagccgccg ttgatccgtc
     1921 ccggctcctt ggggcgcctt gtcgccggat cgtcttcgcc cgtcatcatg actgcgtaat
     1981 ttcccatgtc ctcgccgagg agattcgcct gccagccgaa tgcctttgcg tagaatccga
     2041 ccatcctgtc gcgatcgtcg aagggcattt caaaatgtac taccggatcc atagtgtttt
     2101 ttgattatcg tgtaattcgg ttgaagtata ccatgcgcgc cgtctcggaa tgttccgcta
     2161 caccgatcgt gcagttttat ttcaaaattc gaatgttgaa atattgccgc ttcaggagag
     2221 taataattgc cggattcccg ggaattgccg gttctttggt tctaatttct aaccgaactt
     2281 ttagcagtta atttatgaaa agatcgacaa aaataaccac gggcctcgca gccgccgcat
     2341 tgacgatcgg cttgggcttc tccggagcgg gacttgccca tgcgcaaaca gcaagcacga
     2401 cgcctgaaag ccgcctcgct tctctcgcgc aggcgatcgc atcgaagttc aacctcaacg
     2461 agagcgatgt ggagagcgtc atcaaggacc atcgcgcgga aatgcaggca gagcgcgaag
     2521 aaaggtacaa aagcgcgctc gataaggcgg tgcaaaatag caaattgacg caagatcagg
     2581 aagacaagat cctcgccaag atggaagagc tcaagagcga gagggaatcg cttcagggaa
     2641 agacgccgga ggaaatccgt tcggcgctca aaaccgaagc ggccagcctc aagcaatggg
     2701 cggcggataa taatattccg acagagtatc tccgccccgg cttcttcggc ggtccgggcc
     2761 gcttcatggg tcccttaggt cccaaggacg catcgagcac tgacgctaca agcagttcgc
     2821 aaaactaatg cgaaaaaata cgcaaacgcg ctccgcagga cggagcgcgt tttgctattt
     2881 ccaggttcca aactttattc gcctgacggg gaacttatcg cggcatcctt gatccggacg
     2941 ctcttcgcca cctgagtgat gacggaagcg tcggtctcct tcgcattttt attgagcggc
     3001 ggatggatga cgagcgtcgc gccgtctgtc aggggaaacg cgacatacgg gcctttctcg
     3061 atcggcgtcg atgatcctgc tgaggtgttt ttgatctccc cgcttaggta gaagataaat
     3121 ccccgaagtc cggagtcgga aatgaatgcc tgtgcgcccg cttgatcggc gccggagcag
     3181 gaaagcgatt cgttcggccc gtctgcggcg cagagattcg ccatttctcc gattaccgca
     3241 ttttcgagct cgccgccctc cgcatcgccg gtcttgagta cgcgagcttc cgccactccg
     3301 tcgatcccgt ctccttcctt gtgcccgaac acgacgtcat cctggctgta catccgggtt
     3361 tcgagctccg gcggaaaatc gagctcgtag ccgtacgcgg tattgccgta ggtggcgacc
     3421 ggcggggttg caggagcggt cgattgctct gtccggtccg cggcggacga atcgtttcga
     3481 ttcggaagcc agatggcgag cgcgatgatg aagatcgcga gaatgactgc cgcggtgatg
     3541 atgaaggttt ttttcatatg aaaggcgaaa aagctttagg cgaagaagga gaggatcgcg
     3601 ccgcccacaa gaagcgttac gagcgtattg ccgatggaaa gccagaagag cgatttcttg
     3661 ccgccccaga gtacgtcgcc gagctttatc ggcacaatga agccgatcca ggcgccgaag
     3721 gcatttccga tgccggcagt aaggccggtg ttgatgccgg tattgatgta ccaatcaagg
     3781 acgaagaaca tgacgaagct ggcgacgaac tggatgacgt acgatttcgt catcgacttt
     3841 ttcatccgtt cccgttcctc aggcgtgtga ttctcccagc ccatttccct gatgtacctt
     3901 ttaccgaaaa gggggccgta ccagatgctt ccgactatca tgcttgccac ggcggcgata
     3961 agcaccgcca aaaaattaat gcctgtcata attattgaaa attatttcta atgcagagct
     4021 tccttaatca tagcagaaat cggatcactc cggctacatg gccggttcct tccccgcaag
     4081 cgacggcttg tcttccagcg gaaggctgaa ggagaacgtg gctccttttc ctttctcgct
     4141 ttcgacccgc atctcgcccc cgagcttcct tatgatctgc gaggaaatgt agagtcccaa
     4201 gccgagccct ggatatgtct tgtgctcctc cgactggtag aaccgttcga atatgtgatc
     4261 gagtgcttct tgggagagcc cgatgccgaa atcctgcacg cttacgacga tcattccgtc
     4321 cttttcgcgc gaacgcacga tgacctccga atccttggga gagaacttga gcgcgttggt
     4381 gaggaggttc gtgaaaatct gcgcgatcct gtccttgttg ctcgtgagcg gcttgtccgc
     4441 ctgccagtcg agcgatattt tccgtcccgt cgtgtccctg agttcgccga ctatttcccg
     4501 cgcgatgtcc ccgagaacga accgctcttt ctgaagctcc agcttgccct gctcgagcct
     4561 cgtggtatcg agcatggtgt tcacgagaag ggcgaggcgg tcgagctggt cgttcatttt
     4621 ttccatgaag gcgccctgtt cctggctgtt cgtcatttcc ttgagaagcg actgcgcgta
     4681 gatcttcgcg ctcgtaaggg gagtcttgag ttcgtgcgag atcattgcca cgaaatcgtc
     4741 ttttagccgc tcgagctctt tctgcttggt gatgtcgtaa aaagtggcca cggccgcggt
     4801 gatattgcct ttcttgtcgt aaaccggtga agcattgaaa aagagggtgg ttgaatcgcc
     4861 gttcgggcca gtaatgacga tttcctctcc cgttacaatc tctcccgtcg tgagcgctcg
     4921 cgcgagcggc cattcgcgtg gctcgatttt cttgcctccg atcgtcgccg aaggatacac
     4981 gtattcctcg attgatgaga cgggcgtggc aacggcgggg ccgaggagct cgagagccat
     5041 attgttgcgg ctgatgattt cgccggtggg cgactgcgcg aggagcactc ccacgggcag
     5101 ttgcttcagg agcgcctcca tacggcgccg gtcatgctcg gcggcagccc tcgcctgctg
     5161 ttccttgccg aagagatcat cgagccgctt tcgaaggatt ccgacggtaa acgtgatgag
     5221 aagatacagg cttgtaagga gtatgacatt ttccgtcgct gccactttga ctgagtatga
     5281 tgcggtgcgg gagtatatga aataggtgaa gtagacgatg gaaacggcgg aagcggtaag
     5341 gccgcccctg atcgcatggc ggaatgagaa atagagcacc aggaggaacg tcagcacgac
     5401 aacctccgga cccatgttat ccggtcggtg aagaattaag tttccaataa actcggccag
     5461 cagatatgcg gcggccgatc cgagaagcgg gatgtatatg gagtgattct tttctcccaa
     5521 gctggccatc gggttgaggg aaccccagaa gtgcttgaac atacgcaaat tgtagcacaa
     5581 gcgcctctct atgttgtgaa ccgttatttt gctatattag caacagtgga acgacgtgga
     5641 ataagcggcc gaggcttcgg gcgtcagcag aaccacctcc acctcatgaa tcgaagccga
     5701 ttttaaaagc taatcatttt gccatgagtc tacaagagat gcatgacgaa cacgcgcagt
     5761 actggcgcga caaggacggc ataaagttcg gcctcgcaag catgcgctgg cacggctggg
     5821 gctcgccggt ggggctgggc ctcttccttc tctccatcgg aggttttctc ctcctcctgc
     5881 actttgccgg cctcatcgga ggctaagaca ggtcaaagca aaaacccggg atcgtcccgg
     5941 gtttttgtta ccgacatttc taagaaatct gctccgctta gtctgacgaa ggctcgagaa
     6001 catttcccgg agcgactttg agggtgagcg tttttgcctg cgaaggagcg gtactcatcg
     6061 gctcgttcgg atcgcgatcg gcgtaattga cctcgagctc gagcgagttg gcgggtatga
     6121 ccgtcgactg gggcgcgatt cggtcgccga tgagatatgc attcgtggtt ttccagccgc
     6181 tcgaggtatg gagagcggcc acggcgtagt agaagagccc gctgcctccc gaatcctgcg
     6241 tgacgatgaa tgccatatcc gacgtgccgt cgccgtcgag atcgccggcc gcttcgttgc
     6301 cgaagtagcg cgtcgtcact ttcgaggagg aatcgggaat gggaacttcc gatatgccgt
     6361 ccgcgagagt taccggattt ccatcgatgg caaatgtcga gttgcgcccg tcgaagcttg
     6421 ccgccgaagt ttgtcccgcg gagcccggat ttttcgcgct gccgttctta tgcatgtaaa
     6481 aataaatgcc gattcccgcg ataactagca cgagtatggt gataaatatt tttttcatat
     6541 attcatatta tctagcacat ttcatatgaa gaaaagccga gtttcgggtc gaaaccaggg
     6601 aaatggcaat aatgggaaaa ttttgtagaa tatctgtatg gcaaaaacga tttcctcagg
     6661 gacggtgcat gcattgcctg cagatttgcg gaaggccctt ctttccgaca aggccgcact
     6721 cgctaagtgg gaagacatta cgccgctcgc gcgaaatgaa tggatttgct ggactatttc
     6781 cgtcaaaatg gcggaaacca gaaaggagca tgttgaaaga gtcgtttcgg aactcaagga
     6841 ggggatgcgc cgcccgtgct gctggatcgg ctgcattcat cggccggaaa aggcgataag
     6901 cccttcggtg caatgggcgc tcggcaagcg aactaaaaga aaagtataaa tataaaaaac
     6961 tgctatgaag gaagacgccc agttttatat aggtcaaaaa gcttttatcc gcagaggaga
     7021 tgaaatttta attctcggcg atccgcatga aggcttggat tatcctggcg gcaagattca
     7081 agaaggagaa attgatcttg cggaatcgct caaacgagaa gttagagaag aaacaggcct
     7141 tgagatcgaa gttggcactc cttttgctac ctggacaaac acatttccgc cgcatcatcg
     7201 gctggctggc aagcgcgtgt atctcgtagg atatcgctgc aattatgtgt ctggagacgt
     7261 gaaattgagc gacgagcatg ataagtttcg ctgggtaaat aaggaaaact ataaagaggc
     7321 ggatgatggg acctcatatt ttgagattct tgataagtat tttgaggaat aaaaagaagg
     7381 tataaaaaag cggtctaatg gccgctttgc ttttgaaaac ctcttcgtta tttcctgctt
     7441 gcgaagtgga agcttgcgta ggccaggaag gcggtagcga tcgtcccgag gccgttcagc
     7501 caatagggga cttcccagct gccgagagtg agattccagc caaagacgat gcgggagaga
     7561 tgggcgagag ccaccgccgt aaaaaggacg ccggcgattg ccagaaactt tttctcgcgg
     7621 gaagtgtggg agcggtcttt cgtgttccag ccgtagtgga cgagaaacgc gaataggagg
     7681 acgtcgaaga tgagccaggc gacgaccgtt tgcgcgtcga agcttatacc aaggaacgtt
     7741 atcggcagat agccgccgaa ataaaatccg aggccgcaca ggatatctcc catgatgagg
     7801 cctgatgcga atttcgcaag ttcgcgaagc gttcgtgtat gcatatgaaa attatttact
     7861 gatgagctag taaaattata caacgatttg gaatttcaag atgagcgatc ctgggcattt
     7921 ccagcacgaa attcaaatgt ataatatatg tattgggaaa gctgcaggat caattaattg
     7981 tggtgcaaat ataatttaag tacatatgaa aagaaaccgc cagaaatact cacgaggctt
     8041 cacgctaatc gagctccttg tcgtgatcgc gattatcggt atcctatcgg ccgtcgtgct
     8101 tgcaagcctc aactcagcgc gggccaaagg tgccgatgcg gcggtcaagt cggaccttca
     8161 gagcatccgc aacgaagcgg aaatctttta cgacggcgtt ggaaacaaca cctacggccc
     8221 ggctttgatc acgacgatat gtcccactac cggatcgacg atgttcgtcc aggacgcgac
     8281 aatcgcgaat gcgatcaagc aggcaactgc ggcgaccggc aagaccgtga gcgacgcctt
     8341 ttgcgggacc gatggttcaa accagtatgt cgtagtcgtt ccgcttaaaa cgtcgggcta
     8401 ttggtgcatc gacagctccg gacagtcgaa gtcggaaacg gatgagcaag tagctggcgg
     8461 cagcggctac gtttgtccgt aaaccatttc aaaaccgccg ccctgcaggg cggcggtttt
     8521 ttgttgttta tatggatagg agcaggaaac aggctcaatt tttagaaatt atttgacacg
     8581 aacctatatc atatatatta tgcatgtgct tcgcccgagc tgatccgttg aagacggatc
     8641 gccgggcaat tcggaaaaca atcatacgct ttgccatgag caaaccgaca caccaggggg
     8701 aggaccgcaa gaaacgcatc catcacaaaa tgagcccgcg caaaaggcag tggggcgccg
     8761 aaaagcgcac cgatccgctc tcgcatctcc acgaaacccc gtccgatttt gccgtcacct
     8821 acagccgcat cgcgatcgtc atatcgatcg tgttttggct catgtatctc acctcggtaa
     8881 tcatccgcca gatgatcgac ggcccgaagg actacaactt cacggcccag acgatcggct
     8941 acacggcagt catgtttttc ctgacgctct ccgcgctggt gtacctcatt acgcggcagg
     9001 gctcgcttca gcgcttcaag aagcatattc gcacgccgag gagggtgctc gaagaatttt
     9061 tctccagcaa tcagccgtca atcaccgttc tcattccttc atacaatgaa gaagtgagcg
     9121 tgatcaggaa gacgatgctc tctgccgcac tgcaagaatt ttccaatatc cgcgtcgtac
     9181 ttctccttga cgacgatccg ttcaaggtaa agccggaaga ccgggagcgc atcgacaaga
     9241 cccatgctct gcctgcggaa atcgaaacgc ttttgtccga gcctttgagc cgggcgcagg
     9301 cccttcgcgc cgaaatcctg aatgcggagc gaaagggagg cgaacacgtc ttgaaaatcg
     9361 ccgccgcgta ccattcggca gcggagtggc tggaggcaac ggcggaaaaa gagattattg
     9421 aggatcatgt cgacattttc ttcgccgacc aggttcttcg cagtttggct ggcgatttgc
     9481 gcgccgcggc ccaagacatg acggccgcgc ttcagcgtgg cgtggagttc tcgaacgaaa
     9541 ggctcgaaca gctgtgcaac cgcctggttt ggatttttaa tgcgaagctc ggcttttttg
     9601 agcgcaagcg ctacatctcg ctctcacgcg agaagaacaa ggcgatgaac ctcaattcat
     9661 atatcggcct catgggcgga gcgtatcgaa agctcgaaac ggaggacggt acggtgctca
     9721 tgccggcgga agacgaggcg agcgccgacc tcgtggtgaa agacagcgac ttcattctga
     9781 ccttggatgc cgactcgatc cttctgcgcg agtactgcca gcgcctcgtg tactttcttt
     9841 accagcccga tgccgcggac gtcgcggtcg tgcagacgcc gtactcctcc ttccgcggcg
     9901 catcgacgcg gctcgagcgg ctcgccggcg cgacgactga catccagcac atcattcacc
     9961 aaggcatgac gcactacggc gccactttct gggtcggggc gaatgcggtg atccggaaaa
    10021 gggcgctcga agatatcgtg gaaagggaat tcatcggcgg tttcgaaatc aagcggtacg
    10081 tgcaggatca tactgtcatc gaggataccg aatcgagcgt cgatcttgcg cttcatggct
    10141 ggcggctcat gaattatccc gagcgcctga gctatagcgc gacgccgccg gatttcggct
    10201 cgctcgcagt ccagcgcgaa cgctgggcga acggaggcct tctcattttt ccgaagctcc
    10261 tcaaactgcg ccgcgaacgc aagcatgcgg gccgtccctt gcacatgctc gaattcctga
    10321 ttcgcatgtg ctacatgacc tcgatcgcat ggtcgagcgt tggcctgctt ttcatcctgg
    10381 catatccgta cgacgggcgg ctcttgagcc cgctagtgct cctcgccgcg gcgccgtatt
    10441 tcatcgcgat ggcgatggat ctcaaggctt gccgctaccg ctacagcgac atctttctca
    10501 tctatggttt caatctcctt ttgctgccgg tcaacctcgt tggcacgttc aagtcgctcc
    10561 agcaagctgt gacgaccaag aaaattcctt tcgcccgcac gccgaaagtg aaaaacagga
    10621 cggcgacagc gtggcaatac ctccttgcgc cgcttttcat cgtcggcttc tcgcttttta
    10681 cgatgtggcg caatatcgag ggagagaact ggggcaatgc cgccttcgcc ggcttcaatg
    10741 cgttcactgc ttcgtgggcg atcgtcgcat acatcggcat caagaatatg atcgtagatc
    10801 tctggctcgc ataccgcgac tggctctacg tggaagttcc ggttaaaaaa gcatcgaaga
    10861 aaaagcgcaa ggcgagcgcg ccggagcctg tggaattggc gcctgagccg atagtaatgg
    10921 ccggccaaaa taactaataa atatggaaac gaaagatacg aaaatgcaga atgggaccgt
    10981 acggagattg tcattcgtcc ggctcgccat ttccgttttg gctgtcatcg ctattatcgg
    11041 ttcggccgcg acgagcttcg ccgactggca gcaatcggct gagctcggca agaccggctt
    11101 tgctccgtgg tttgccgcgt atgtcgacgt cacctcgacc ccgacgtatc cgttcgagca
    11161 atacggttcg acggcagagc tcaataacgc agtgctcgca tttatcgttt cctcgccggc
    11221 acaggcatgc acgccgacgt ggggcgggca gtatacgctg gacagcgcgg gggaaatgct
    11281 cgatctcgac cgccgtatcg cccgcctcga acagcagggc ggccgcgtcg cgatttcgtt
    11341 cggcggcctc ttgaattccg agcttgcggt gaagtgcacc gacgcggcac agcttgcaaa
    11401 agcatacaaa tcggtgatcg accgctacag cgtgagcacg atcgatctcg atctcgaagg
    11461 ggacggcctc aatagcgctg atgccgtgaa gcgacgcgcc gcggtaatcg cggagctcca
    11521 aaaggattat cgaagcgagg gcaaggctct ggccgtgtgg gtaaccctgc ccgtgactcc
    11581 gcaaggcctc tccaaagagg gaacagatgc tgtcgcagaa atgctttcaa gcggcgttga
    11641 tcttgccggc gtcaatgcca tgaccatgga ctacgggcag gcgctcaatg cgaacgattc
    11701 gatggccgcc gcatcggaga aagccctcct cgagacgcat cgacagctgg gtatcctcta
    11761 ctcggaagcg ggaatcaatc tcaacagcga ctcgctctgg cggaaaatcg gagcgacgcc
    11821 catgatcggc cagaacgata ttgccgacga agtgtttacg ccagatgacg ccgtagagct
    11881 gaataaatac gcctttcgcg tggggatagg ccgcatgtcg atgtggtctg ccaatcgcga
    11941 tgtcgcatgc ggcaagaatt acgtgaatgt aaaagtcgta tcggatgtct gcagcggcgt
    12001 tgatgccggc cccttcgctt tcgtgcacgc gcttgggcag ggattcgtcg gcgacatcat
    12061 ccaaaacgcg acgatcgtca cggtcgcgga tcccgacagc aataagccga ttgtcgacga
    12121 tccgctgacg agtccgtacc ctgtttggga gcagagcgcg gtgtacctta aggacgcgaa
    12181 ggtagtctgg cacggcaacg tgtatcaggc aaaatggtgg acgaagaacg acgttcccga
    12241 caatccggtc ctgcagtccg cggatactcc gtggcagctc atcggtccgg tcctgcctgg
    12301 cgataagccg gtgaagcttc tcacattgcc tgctggcacc tatcccgact ggcgcggcga
    12361 ggcgatctac aacgagggcg atcgcgtcat cttcaacggc atgccgtacg aagccaagtg
    12421 gtggaaccag ggccaaagcc ctgccgcggc cgcggtcgat ccggataatt ctccatggat
    12481 catcctcact caggagcaga tcgaagccat ccgcgaagcg cggggattgt agcgatttgc
    12541 ttgcagagta aataattcgt tttttacacg cgatctgtgt ggataagttg ctttacagaa
    12601 attccgcttt cattacgaat tacgagcgta tctcaatatg acgccgccgg tttggagcgg
    12661 ccgcgcttcg agcaatttga gtttctgata cggcaggcct tcgttgaaga ggcggccgcc
    12721 cttgccgagg aaaatggggg caatgcatag gcgatactca tcgaagaggt ccgctttcat
    12781 aagcgattcc gaaagctttc cgctcccgaa tacgaacatg ttcccgtcgc cctcttcttt
    12841 catcttcgaa agttcggcaa ccgcatcgcg cacgatcgtc gtgttgttcc aatcggcagt
    12901 ggcgagcatt gtcgagcata cgatcttcgg aagcttattc atatattcgg ccgtctctcc
    12961 cttggctttg ctccagtagt cggccatgcc tttgtaggtt ttctctccga aaacaatcgt
    13021 gtcgattgtt ttgagctgtt cggcgacaaa ttgctccatt tccggccccc acaccaggcc
    13081 atgaaaatcc aaatcccatg ccttctcacc ctcgaaatat ccatcgagcg tgatcacgtt
    13141 ccacatgatg agttttctca taaattctct ggttagatac tattgatttt gttagtgttt
    13201 tgaataatac gatatctcca atcacaatcg gccgttcatg cctattctgc ttccgcaaga
    13261 gcttttatgc gctccagcac cttgggaagc gtgttttcaa aatattcgac tagctcgtcc
    13321 ggcacgtctt ctattattga gagcgtgacc tggccatcat gctcttccag ctcataactc
    13381 tcgacgcccc cagtccactg atcgtctctt ttttcgatcg agccgtcatc ggcgaccttg
    13441 atgtcctcca cttttctgaa tgagataagc ttaagaggaa tcaatttctc cactttgctg
    13501 cttactccat acctgacctt tccatcgctg tttccgataa aattaacctc cgctccttcc
    13561 tgcagagtgc ccgtcatgta cgttccttgg tctatgatat tcgcccaatc cctgaaagtc
    13621 ttatcatccc aaagcacctg ccaaatcttt tcttttggag cgtctatttc aattgaatgt
    13681 cgtatgcttt tcatatagtt aagaatactg cagttagaaa attaattcca tgtgagttat
    13741 gggcaaaaca ttgctatgaa aataatgccc gacattctaa atgtcgggca ggcaagctag
    13801 cggagaggta aaccgagttt cttccatttt tccaagagat atggaatgta agatgaatta
    13861 ggatcaacct ctctttttct tttgaggtac agaaggcgtt gtctttttga ccgtggactc
    13921 tcggaagcct tcctattact ggattttcgg gctttttgtt ttgccgccac ggagaactcc
    13981 tttctttttg gattccatac caagtatacc ttgcccagac tttccatgca ccggctccat
    14041 aacattgatc gaaaggtgga atatgcttgc cacccagatc tggtcttcga gaggtgcgcc
    14101 acgatccttg ttcagctcga caatcttatt ccaaaattct cggacaagat agccgaaaat
    14161 aaacaatccg cagtctacga tccattttgc catcctgggc gcgagacgtt tgtagagctt
    14221 tcttgcgaac tccttctttt ccttacccca aaatgaagca tgtcgataga aaaatcgttc
    14281 aaactctggt gctgcccata ctctataatt atcagtatga gcatactcgg caagaataaa
    14341 cgatagaatc gctcgataaa catcgggata acgatgcttt cgggcctaag cttcggcacg
    14401 cttgtcttcg aatttgaaca tgagccgaat gacttgcgtt cgctcttcgt cagttatgat
    14461 tgtcccgttt cttttgatac ttttgagaaa cacaccatcc acttcgtaca tagaccagcc
    14521 atagaagacc tggccgcaca tcatcatgcg agcttttgct tcgtcgagct tagttaaata
    14581 ttgctcatcg tcaccgtagc ggtttggatc aaaccaatgc ggatatgcag acatctcacg
    14641 ccttaagcgt ccggcatgat cagtgagata tttgcgcaca tgatcaacat caagtccgtt
    14701 ctcgagtgtt tcgtatattc taccttgtgt cgctactcgt cgagggaaat atacttctgc
    14761 aagaatactt cggcgtcctg gttcgaatgt gtaagacgaa tatggttcaa gttgcacttg
    14821 aattctcctt tctgtatgaa aataacacaa tagtaatttg tgtcaaatac taattttgag
    14881 agtaggaatc tcgaacctta caggagctgt acacctttcc ttacaggaac tgtccactca
    14941 ttcgctctcg aagactcgag ctgaatgagt cttgcgaaag ctatgtatac tcgctttctc
    15001 acccgacccc ttcccgccct agccccttcg caaaataaaa aa
//
LOCUS       DASZYS010000019        22986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_985950, whole genome shotgun sequence.
ACCESSION   DASZYS010000019 DASZYS010000000
VERSION     DASZYS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 22986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22986
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985950"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..714
                     /locus_tag="VHF05_01160"
     CDS             391..714
                     /locus_tag="VHF05_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79563.1"
                     /translation="MERSLRRPTMAETITLKRVESIPFDKSTMRVNIPEVPDAYVQWE
                     GPSGPDESIDLHLPNREPQGLGYDRIFTVRTRYGPDAIYQITHKKDWNRDWWIYLERF
                     EQELD"
     gene            complement(994..1464)
                     /gene="rpsG"
                     /locus_tag="VHF05_01165"
     CDS             complement(994..1464)
                     /gene="rpsG"
                     /locus_tag="VHF05_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HVS79564.1"
                     /translation="MRRKVKAKRPLPADAVYDSVKISKFINYTMERGKKNAARKIVYD
                     ALNIIKEREKIDDPLEVLETAFKNTGPTMEVRSRRVGGANYQIPREVRQERRSFLSMK
                     WIIDAARAKKGKPMAEKLAEEIMLAAKNEGEAVRKRENTHKMAEANKAFAHFAW"
     gene            complement(1470..1892)
                     /gene="rpsL"
                     /locus_tag="VHF05_01170"
     CDS             complement(1470..1892)
                     /gene="rpsL"
                     /locus_tag="VHF05_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HVS79565.1"
                     /translation="MSTINQLVKRKRKNVARKPKAVALRRGFNAVTNRPVFYASPFKR
                     GVCTRVTTKTPKKPNSAIRKIARVRLTNGMEVTAYIPGIGHNLQEHSVVVLRGGKVKD
                     VGLRYTIVRGVLDTSGVETRRQGRSRYGNKKPKAGAKK"
     gene            complement(2316..2942)
                     /locus_tag="VHF05_01175"
     CDS             complement(2316..2942)
                     /locus_tag="VHF05_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVS79566.1"
                     /translation="MQITPLFSVFSLVILIMSAAVHEVAHGWMADSLGDPTAKYQGRL
                     TLNPLKHIDPWGSVVVPVLFYLASGGAIGFGWAKPVPYNPYNLRDQRWGELKVALAGP
                     AVNFILAALGSIVIRAVVAGGVQNEALILALAAIVTVNLVLGLFNLIPLPPLDGSKIL
                     YAFLPFKYYAFRQNLEYIGPIFLLVIIFFLWPVISPIIGALFGLFTGL"
     gene            2993..3235
                     /locus_tag="VHF05_01180"
     CDS             2993..3235
                     /locus_tag="VHF05_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HVS79567.1"
                     /translation="MAKVCPVLNKTSQVGGGYSNRTRATKFNPTGVRRRQPNLQKKKI
                     YIPELKKSVIVTISTRALKTIQKNGAFATLHKAGLV"
     gene            complement(3321..3830)
                     /locus_tag="VHF05_01185"
     CDS             complement(3321..3830)
                     /locus_tag="VHF05_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79568.1"
                     /translation="MLEKIDARHVAALRDEMFSKRERVATPFPWVKKQLERLRESNNR
                     EIFNFIEGLVRDHSYLDNAMRWRIESLAVIAYRLVSIRYHGLCPQITSEARIQFEKEM
                     QSESALTSLCGNLCQHDPLFAHEISCPGGSTSRIKPFEAAGAFVYGLIDYQVNRMRRL
                     APSRIPVLA"
     gene            complement(4503..5351)
                     /locus_tag="VHF05_01190"
     CDS             complement(4503..5351)
                     /locus_tag="VHF05_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVS79569.1"
                     /translation="MTRKRILLALVAFAALLNALVWYAVLRERPHDLKVSVLDIGQGD
                     AILIESPTGNQMLIDGGGTREVLQRLGEEMPFYDHSIDVVMETHPDKDHIGGLPLVLS
                     AYQVGAFMEPGIESDTKIDDELEKDVDADHVHRVIARRGMVVHLGGGADLSILFPDRD
                     MSGYETNEASIVAKLQYGATSFLLTGDSPSNIENYLIGLDGEGLKSDVLKVGHHGSKY
                     STSLLFAKEVDPEFAAISVGKDNSYGHPSQEALNNLAAVGAQIDETENEGTIHFLSDG
                     KTVIKE"
     gene            5441..6043
                     /locus_tag="VHF05_01195"
     CDS             5441..6043
                     /locus_tag="VHF05_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HVS79570.1"
                     /translation="MTKFEEQKFTIPAELKGISQKTMEEHLKLYAGYVKNANLVQEHI
                     AELSKDAEKNAYELGELERHFSFEFNGMRNHEVYFASFEGGPKELSAESPLRAAIAAA
                     WGNFDAWLAKFKSIAATTRGIGWTALWYDPASKNLLNSWIDEQHLGQLQNCKMILGLD
                     MWEHSYVADYQPSGKKQYIEDFFANINWSVIEKNYIEAAK"
     gene            complement(6139..7674)
                     /locus_tag="VHF05_01200"
     CDS             complement(6139..7674)
                     /locus_tag="VHF05_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HVS79571.1"
                     /translation="MTLYLFSACAALGIITAKILSLPIPFSLLAFLAAGAIFAYAKFA
                     KETPAQLALVAIILFGFGMGACRMAIAERPQPDPLAAVAERIVTLTGVVNADPDERDS
                     YTQLVVNISEAEVGTRKIRTHEKILARVPITSGFRYGDLISISGKLERPQNFETDSGR
                     EFDYADYLGKDGIRYQMSFAEASLLSHGHGNPILAGLISVKRSVEEKILRVIPPPEGS
                     LANGVLLGAKQSLGQSTLDDFKLAGLSHVVVLSGYNVSIVARIVVWIFSFLPSMAALS
                     AGALSIVLFSMMAGGGASVVRAAVMALISLFARAIRREYNALRALVLAALAMVLQNPR
                     IVTDDPSFQLSFIATVGIISVTPIIMRYFRFVPEKRGFRDTVATTVSTQLFVLPFLVY
                     MSGLVSPVAFPANLLVLPLVPFAMLGVSVTALVAYVSQFAALVPGFASYGILAYILTV
                     THAAAHVPFGSFAIENVSGWFIALWYALYAGLLWKWHAKPRKREIETPKFDTSLAFEI
                     VEE"
     gene            complement(7820..8539)
                     /gene="rnhA"
                     /locus_tag="VHF05_01205"
     CDS             complement(7820..8539)
                     /gene="rnhA"
                     /locus_tag="VHF05_01205"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HVS79572.1"
                     /translation="MALQKEIIIFTDGASKGNPGPGGWGAIVATNAEVKELGGREENT
                     TNNRMELRAALEALSFASLLHDAPVVLHTDSSYVINGVTKWSHGWKTRGWVTKEKREV
                     LNQDLWKDLLDAAENFSNGLAWQYVGGHVGIAGNERVDEIASDFADGKKVELYSGPLE
                     GYGVDVKNISLDESKKKQKSASRSRSKVAAYSYISEVEGVVMTHATWRECEARVKGKR
                     ARFKKATSAEEEAEIIKTFSK"
     gene            complement(8695..10464)
                     /gene="recJ"
                     /locus_tag="VHF05_01210"
     CDS             complement(8695..10464)
                     /gene="recJ"
                     /locus_tag="VHF05_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HVS79573.1"
                     /translation="MKATKAAGTGQKRWKSSKNEYILRDLPEHAELGVASPLTGFLLR
                     TRGIETPEDAAAFLVPDYALHIHDPFLLKGMEKAAERILSAIKKGERITIWSDYDADG
                     IPGGVILHDLFAKIGYENFTNYIPHRHHEGFGLNIAGVEKLAEEGAKLIITVDCGIAD
                     VKEVARANELGVDVIITDHHLPGEKLPEAFAIVDPKQEGCTYPFKELCGSGVAWKLVQ
                     AILAKDRLAKNRFGIKEGWEKWLLDMVGLATLSDMVPLVGENRVLAFFGLKVLRRTPR
                     VGLLKLSRELRLDLSHLTEDDIGFTITPRINAASRMGVPMDAFELLATTDVLEAGRLV
                     AHLDSINSERKTLVAAISREAKKHCAEKFESEEMKRVIVLGNPDWRPALLGLVANTLV
                     EEYNRPVFLWGREGGDGLKGSCRSGGSVNVVELMQHAADALSDFGGHKMSGGFSTTIE
                     KVHLLDDALNSAFSKFRDELSAREVCTIDRAMKLDDVTWNTYGEIEKLAPFGLGNPKP
                     LFMFENVEIGNVRTFGKGNLHLEMIFENSRGNPVKAIAFFTKPEDFGIELAAGKRVNL
                     VAHIEKSVFLGRIELRLRIVDII"
     gene            10611..10991
                     /locus_tag="VHF05_01215"
     CDS             10611..10991
                     /locus_tag="VHF05_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVS79574.1"
                     /translation="MENCIFCKIVRGEIPSHTIYETDDFLAFLDINPSSPGHAQVIPK
                     KHYRWVWDVPNAGDYFEAAKIVALAERRVWKTDWVLSKIVGDEIEHAHIWVYPHRDVA
                     GDPSNFVENARLLRAALEEIKMGK"
     gene            complement(11542..12654)
                     /locus_tag="VHF05_01220"
     CDS             complement(11542..12654)
                     /locus_tag="VHF05_01220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVS79575.1"
                     /translation="MKTLKEIEDIKDKKIFLRCDFNVPVTDGKIDDDYRIKKALPTIH
                     ELREKGAKLILASHIETEGNPTLAPVRDYLSKIFPVDFITDFFPEDIAERVNVLPEGG
                     VILLENLRKYSGEKANDESFAKHLASFADLYVDDAFATAHRPHASIVGVPKFIQGFAG
                     FLLTDEVTRLASAFNAPHPFVFILGGAKFETKLPLVSKFLGEADTLFIGGALANDIYK
                     ARGWNVGASKLSKDADVSAFATNEKIAVPMEVVVKASAKQSEKSVKEIGDDEMILDAS
                     VKSLELLRQKIADAKFILWNGPLGVFEEGFRESTEALAKMIAESPAESIVGGGDTLSA
                     IQSLHIEDKFSFVSTGGGAMLDFLANGSLPGIDALG"
     gene            complement(12666..13436)
                     /gene="tpiA"
                     /locus_tag="VHF05_01225"
     CDS             complement(12666..13436)
                     /gene="tpiA"
                     /locus_tag="VHF05_01225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVS79576.1"
                     /translation="MKKKKRMFIAGNWKMNPQSLEAAKEVFRQIKKSAAKLKNTDVVI
                     CPPTLYLSELKKIAKDDFALGVQNIHWELSGSHTGEISARMAADVGAHYSIIGHSERR
                     AMGESDEEVSKKIAAAFAEGLVGIVCVGERERDTEGQYLEFLKNQIEHSLAGVSKSQV
                     KRLILAYEPVWAIGRKDNAALGGKELYETVLYIRKVLLGIYGKDIAFGIPVLYGGSAT
                     FQNVGDILSEGEVEGLLVGRQSLEPEGFAQLLRIANSL"
     gene            13667..15043
                     /locus_tag="VHF05_01230"
     CDS             13667..15043
                     /locus_tag="VHF05_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVS79577.1"
                     /translation="MQETTSAVLSETFETGAEKNWWKNAVFYEVYVDRFAGTFADFSK
                     RLDYLANLGITCVHLLPHYPSPMVDDGYDVSDYRDVRPELGTLGDFSHFVQAAHSRGI
                     RVMIDFVLNHVSTSHPWFVEASSSKKNPKRDYFLWSETGEEFATTPSPFPDLRESNWV
                     RNPATGDYYFSTFYPEQADLNWDNPRVFDEMTGVIDFWAALGVDGFRLDASSHLIKRE
                     GTNSHGLPETHAILKRLRAHIEKLGPDIAILAEVHDSLAEMIRYFGDGDECHMVYHFP
                     MVEQIFLSFIRGTSAGIEKLAELSAGIPDNCSWALFLRHHDEISLATLAPGVRAELLD
                     YYDPERKWRFSEGTSLRLSDMMKNDIGKTLDAYRLLFGLPGTPFIYYGDEIGMRNVPL
                     APAEKDTRRALRGKFDWAAALIAERNPASLLRQVAMLLKARRHMKPTEESIPERTAET
                     EEKIPAAV"
     gene            15102..15428
                     /locus_tag="VHF05_01235"
     CDS             15102..15428
                     /locus_tag="VHF05_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79578.1"
                     /translation="MSIDEFAALLKNLPRDKYFPYINGHKRIFIGTRIGNITFTVLQG
                     AAAFMLNEYENFTKPTNLHVLAYMLKLSYEDTKLIAHATHNDLDLARAAVVRGKILDA
                     LGLKEE"
     gene            15500..15964
                     /locus_tag="VHF05_01240"
     CDS             15500..15964
                     /locus_tag="VHF05_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVS79579.1"
                     /translation="MFKQLLQLFKPFIMPACRAYWFIVRPETAGALIVLTNNGEMLLL
                     THTYRYGWGLPGGGLKRGETPEEAVRREVFEEIGIALSAATPLGSFLSTDDYHRNTVY
                     AFAAALEKRDISTENFEIESAQWFLPENLPPLGPVTKKVVELYKNSIYRKEI"
     gene            complement(15985..17430)
                     /locus_tag="VHF05_01245"
     CDS             complement(15985..17430)
                     /locus_tag="VHF05_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79580.1"
                     /translation="MQSIKKQKAAKGTILVQTLVFSALAVLLMSALAVGAGAAVKASR
                     VATIREQAIQVAEAGIDYYRWHLAHAQSDYQDGTGAAGPYVHDFYDAAGEKVGTYTLT
                     ITPPPTGSTIVTIQSTGAMDANPEVTRTIVAQLAIPSLAKYAVVANDDMRFGEGTEVF
                     GPIHSNGGIRFDGVAHNVITSALSSYNDTDSDDCNGNSSFGVHTCVSPADPRAPAAVP
                     SRSDIFMAGRQFPVPAVDFVGLTTDLSAMKADAQSGGAYYANSGKQGYHIILKTNGTY
                     QLYKVTSVKGATSNCMNWSGASGTDGWGLWTIQNQTLIGTYSYPSNGIIFVEDNLWVD
                     GQIDNERLTIASGRFPDNPSTRTSITVNSDLLYTNYDGADVLSLIAQNNFNVGYSSDN
                     DLEIDGAIIAQNGRVGRFYYASQCGTSYVRDTITLYGMIGTNERYGFAYTDGTGYQTR
                     NLNYDANLLYGPPPSFPLTSDQYQIISWKEI"
     gene            complement(17570..18256)
                     /locus_tag="VHF05_01250"
     CDS             complement(17570..18256)
                     /locus_tag="VHF05_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVS79581.1"
                     /translation="MNRTPLQKGFPCSRFRFGSGGGRNGRSPKSKFTTGFTLAELVIA
                     IAILVVVGLAVSTFQKDVFSLAFNFQGNLDFQLGARAMLKSFTSEAREASPSSVGSYP
                     IEEASTSEFIFYSDMYNDGKKERIRYFLSGTDFKKGITIPTGSPLSYNIATEKTSTLL
                     SNVSNGTSSIFEYYDTNYTGTSSPLSQPVDITSIRLVKATIYAKPDARSSSSEEFTTE
                     VSIRNLKDNL"
     gene            complement(18259..20127)
                     /locus_tag="VHF05_01255"
     CDS             complement(18259..20127)
                     /locus_tag="VHF05_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVS79582.1"
                     /translation="MRRVSFGFPCSRFRLGSVHDRNGQSPKPKFTTGFTLVELLVGSA
                     VFLIVALSVYQSYTSIYRTVEASRYKVDAAALANEELEIVRNMPYSDVGVENSIPDGV
                     LAHSQVLNRDGADFTVVTTVRNIDDPFDGTLGGTPNDTSPADYKLVQLDISCEACKNF
                     TPISVATHVAPKNLETASTNGALFIKVFDGNGNPVADANVHVVNDTATSTITIDDVTD
                     QGGMLELVDVPPGTDAYHIVVSKSGYTTDQTYAPGDLGSSTPSHTDATVLLQQVTQTS
                     FSIDKVSTAKITTVTPTCSAVGNIGFTYAGSKVIGTSPDVPKHTVAATTNSGGAYSDS
                     NVEWDTYTFAMTDSSYELLGMNPVNPVTIGPGASQDIQMIVHSKTPQSVLVSVVDSGT
                     GLPVSGASVEITGPDGFDETETTGEGYLRQTDWSGGGGQNDFADETMWSTSDGNVETG
                     EPEGELKLANRLGQYESSGTLTSSTFDVGSSTNYNEISWTPLDEPPSAGADAVKFQLA
                     ANDDDLTWVYTGPDGSTSTYYTLSNRTIAPSLSGHRYIRYKAFLSTDDTSVTPNVSDV
                     AFTFTTACTPPGQVIFTNLETGTYTITVTKSGYQDYSIGHSVGSTWSSANISLNPE"
     gene            complement(20249..20911)
                     /locus_tag="VHF05_01260"
     CDS             complement(20249..20911)
                     /locus_tag="VHF05_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79583.1"
                     /translation="MKIKTAYYILAGIVFLFAIMALFLVSWMGKLNNNMISDTTSSTT
                     TGTPKTYHSDKYGIEFEYPENVRIDEGQNMHNSSLDIGEISYYSGVNTDPKGFGIRIA
                     TTTVQDVSNLEFDYQKGVSKDNPEIQSIKITNGTTPVIESSEIITVAGKQALKEVISD
                     GNTLHPSESTWVHIINNGKLYSLYSGISHGYDGTPYAATPQDEATVQQYLNLFLSTVK
                     FF"
     gene            complement(20999..>22986)
                     /locus_tag="VHF05_01265"
     CDS             complement(20999..>22986)
                     /locus_tag="VHF05_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79584.1"
                     /translation="KTAFRCRCRSLKEKGENVRSTLSLLFIMACCLLCGSNANATEYL
                     VWGNDTLTSGWSVAAANGATITEEPELYDYLVTSSSAHGTIEFTTSTNFSTSGCTELI
                     VNVTNNDSSGSAGDIIIHLYDGTGAEIGTGVHATDYFGATLRQNTPMDMYIPLYRWSS
                     SPMTISKISFEMSAASTMQVAIVQFYDEQMPDPYPLYIYHYSLYSGWTSTNSSGASVD
                     EDNTGSSSFYINPYHIRATYSVANGIVDLDKSSGYVNVNSYDSLAFDMGVSASPDILV
                     ALRDENGNIFTDQGSPANEIWQAVKMHCLDGSGSPGTWPSFGCRAQIPFAMLGVGEYG
                     NNTLVSGVAFKSPASGVGNVDISDIRFVNGYSTGGYMQFPLKGSFGSTLMTPYNTEVF
                     AVMDHNDDENISTTGDANSIVAYNGEEGLASNGDQNDSGLHGYKKNGGAQFLLPLLTY
                     RDEHPEVNTNQHTFLFYENGSGSGHRGYDYAHSGIDGADVHAAEYGVLCVATDNTSYD
                     GTNVWRDPSNCKLHDLNNDQWSKYHAVYIIHDKGVWGSDSSTFPNTGDSSRGCFTGNY
                     WRSYISVYLHMSDLDHPSTGFDLRQQIIDHGYAVVKRGQVIGQASDYDTQEIIPPHLH
                     FGFYIGCTPSDPYGPGSGDSNGGIPSTFRVLWEVQPQ"
BASE COUNT         5416 a         6096 c         6120 g         5354 t
ORIGIN      
        1 gattaacatg tggaaatgcg agcaatggaa tggaaacgga gttgcaaact gaaacgaatg
       61 agtaaatgac acctgagagc ctctccgtgg gggaggctct tttatttttg gtctgtgaat
      121 acttggtttc ttcactatgg catggtacaa aagcggaaga acatgccaga aaacgatatt
      181 gatctttcaa tactgaccga tgcggaaacg ccgacgcata tagagatgcc cgagaacata
      241 gacctcggaa cattgcttga gccggctgac gaaccggccg acatcgaacc gcaatcatac
      301 atacgaccgt tttagaattg cgacgtttcc tcttggagac acttagcgga acccaccggt
      361 tccgctttta tttgccaaaa ttttataaac atggaaagat cgttgcggag gccaaccatg
      421 gcagaaacta ttacgctgaa gcgtgttgaa agcattccat tcgacaaaag cacgatgcgc
      481 gttaatattc cggaagtgcc cgatgcatat gtccaatggg aaggaccatc tggacccgat
      541 gaatcaatcg acttacatct cccgaatcga gaaccgcagg gcttgggtta tgacagaatt
      601 ttcactgtac gcacacgtta cggaccggac gcgatctatc aaatcactca caaaaaagat
      661 tggaatcgcg actggtggat atacctagag cgattcgaac aagaactaga ctagaagcgg
      721 ggcaatagcc ccgctttttt aattgccgta cgaatttggc tttgcaaatt cgttccacca
      781 ggcgaaatac ttgtcccagt gccaattttg catgcaatcc tggggcggcc cgcttcgccc
      841 ggaaggaaag cactattcgc gcacgcaggc cgctttttca aaaaaatttt tcatgggtag
      901 aaaaaattgc cgagaataaa ctacggcaaa attggtacgg gacgctgcat tttgcagttg
      961 gaaaataaaa accgcccgag ggcggttttt attttaccaa gcaaaatgcg cgaatgcctt
     1021 gttcgcctcc gccatcttgt gggtattctc cctcttgcgg acagcctcgc cttcgttctt
     1081 cgcggcaagc atgatctcct cggcgagctt ctcggccatc ggcttgccct tttttgcgcg
     1141 ggcggcgtcg atgatccact tcatcgagag gaacgagcgc ctctcctggc ggacttcgcg
     1201 gggaatctgg tagttggcgc ctccgacgcg gcgggagcgg acttccattg taggaccggt
     1261 gttcttgaac gcggtctcga gcacctcgag aggatcatcg atcttctcgc gctcctttat
     1321 gatattgagc gcatcataga cgatcttgcg ggcggcgttc ttcttgccgc gctccatcgt
     1381 gtagttgatg aatttcgaaa tcttgacgga gtcgtatacc gcgtctgccg gcaacggcct
     1441 ctttgctttg actttccttc gcatagtgat tatttcttgg ctccggcctt aggcttcttg
     1501 tttccgtagc ggctccttcc ctggcgcctc gtctcgacgc ccgacgtgtc gagcacgccg
     1561 cggacgatcg tgtagcggag gccgacatcc tttaccttgc cgccgcgaag cacgacgacc
     1621 gagtgctcct ggagattgtg gccgatgccc gggatgtatg cggtaacttc catgccgttg
     1681 gtaaggcgga ccctcgcgat cttgcggata gcggagttcg gcttcttagg ggtcttggtg
     1741 gttaccctcg tgcatacgcc gcgcttgaag ggagatgcat agaaaacggg gcggttcgta
     1801 accgcgttga aaccgcggcg gagcgctacc gccttgggct tgcgcgcaac gttcttgcgc
     1861 ttcctcttta cgagctgatt gatagtagac atttcaacaa aaaatgctgt ttttcagcag
     1921 tgcgaatact ttactatgtg cccttaaaaa atgcaagcaa atgaaaaggc ggctcttcaa
     1981 gaagggccgc ctggaatggc ggtgaggccg catcggtgcg tttgtggctt ctgcagctgc
     2041 caccagtaca gccgagattg ccgaagacgc gagcctcacc ggatattgag catagtttaa
     2101 ggaaattcac ctgtcaagaa ctcctcctgg ggatccgcta aataaaaagg cgattcattc
     2161 ttaaattaga aagaatcgtc gtaaatggcg gcgagcgcct ctcggggggt ggggcgcccc
     2221 attgcgccct ttaacgaaac gcaatggggt ggaaaggagg cactcgccca ctacgagcat
     2281 accccttctc acgcctcagt caaggaagag gtgctttaaa ggccggtgaa gagaccgaag
     2341 agcgctccta taataggaga gataacgggc cataggaaga aaataatgac gagaaggaaa
     2401 atggggccga tatattcgag attctgcctg aacgcataat atttgaacgg aaggaaggcg
     2461 taaagaattt tcgaaccatc aagcggcggc aaaggaatga gattgaagag gccgagcacc
     2521 aggtttacgg taacgatcgc cgcaagcgca aggatgagcg cctcgttctg cacgcctccc
     2581 gccacgactg cccggatgac gatacttccg agggcggcaa ggatgaaatt caccgcaggg
     2641 ccggcaagcg ccactttgag ctccccccat cgctgatccc tcagattgta gggattgtag
     2701 ggcacgggct tcgcccagcc gaagccgatg gctcctccgg aagcgagata aaacaagacc
     2761 ggcacgacga ccgatcccca gggatcgatg tgcttcagag gatttaaggt aagccgtcct
     2821 tggtattttg ccgtcggatc acccaaactg tcggccatcc agccgtgcgc cacctcgtgc
     2881 accgccgccg acatgatgag aattaccagc gaaaaaactg agaaaagcgg cgttatttgc
     2941 ataatattca ctttatgata ccatacgggc tacgtaaatt ttaggaaaac ccatggcaaa
     3001 agtctgtcca gtgctcaata agacgtcgca agttggaggt ggatactcca accgcacccg
     3061 cgcgaccaaa ttcaacccta cgggagtgcg ccgccgccag ccgaacttgc agaaaaagaa
     3121 gatctacatc cccgagctca agaaatcggt cattgtcacc atttccacca gggcgttgaa
     3181 gaccattcag aagaatgggg cgtttgctac cctccacaaa gccggcctcg tctaaataaa
     3241 ctccccttaa tttgtcggat ataaaaggaa gtcccggaaa cgctatgttc cggggcttgt
     3301 atttttgtgc tatgcaaatt ttatgccagc actggaattc ttgatggcgc aagccggcgc
     3361 atcctgttga cctggtaatc aataaggccg tacacgaagg cgcctgccgc ctcaaacggc
     3421 ttgattcggg aagttgaccc gccagggcat gatatctcgt gggcaaaaag cggatcgtgc
     3481 tggcagagat tgccgcagag cgatgtgagc gcgctttcgc tttgcatctc tttctcgaat
     3541 tggatgcgcg cttcggaagt gatctgaggg cataggccgt gataacggat agagacgagc
     3601 cggtacgcaa taacggcgag cgattcgatt ctccagcgca tcgcattgtc aagataagaa
     3661 tggtctctga ctaaaccttc gatgaaatta aaaatctccc gattattgct ttctcgaagg
     3721 cgttccagtt gttttttaac ccacggaaac ggggtggcca ctcgttctcg tttggaaaac
     3781 atttcatccc gcagcgcggc gacatgacgg gcatcgattt tttcaagcat gcagctctcc
     3841 tttctttctg atttcgattc tatgcctctt tcgcaaaaac acaaggagcc gcaacggctt
     3901 tcccgcaggc cggatttcat ctttcattca cgcggcgctg ttatggtcga tatggaggag
     3961 gtatagaact tttggcagca cccgcacaag cacaaattcc aacggaacag gagcacctgc
     4021 acggctcgga aatcgtattg aagaattttc cgtgcataca atgcgacaaa tccgatcaga
     4081 aagtgatcga catgaggtat tcggacgtta ccaaaccgca atacgtatac atcaccgcgt
     4141 catgtccgaa ctgcggatcg cacaataaga aaacattcat ggcataaatt gaatgtttgc
     4201 gcacgaaagc gccggcagac cggcgctttt atctttttat ctgcatggaa gttgtggcac
     4261 ggtagagagg gagattgtga tggtaaaaat ctttaagaaa cgcgaagaac tgctcgtgac
     4321 acgatggtac tgcaagcact gcaagcaagt tacagaccag gaaattatcg cgtccagcaa
     4381 caccccgtct catatagtgc tgacgattaa atgccgaaat cctgagtgca accttgagga
     4441 tagaattcct ctcgctctca attaaaccaa acaagaggcg ccgcacgtaa cgtgcagtgc
     4501 ctctattctt ttatgacggt tttcccgtcg cttagaaaat gaatcgtccc ttcgttctcg
     4561 gtttcgtcga tctgcgcgcc gactgcggcg agattgttta aagcctcctg cgacggatgg
     4621 ccgtatgaat tgtccttgcc gacggaaatc gctgcaaact cgggatcgac ttccttggcg
     4681 aacaggagcg aggtggaata tttcgagccg tggtggccca ctttcaagac atcgctcttg
     4741 aggccctccc catcgaggcc gatgaggtaa ttttcgatgt tggacggcga atcgccggta
     4801 aggaggaacg acgtcgcgcc atactggagc ttcgccacga tcgacgcttc gttcgtctcg
     4861 tatccggaca tgtcgcggtc gggaaagaga atcgagagat cggctccccc gcccagatgc
     4921 accaccatgc cgcggcgcgc gatgacgcga tgaacatgat cggcgtcgac atccttctcg
     4981 agttcatcgt cgatcttcgt atcggattcg atgcccggct ccatgaacgc gccgacctgg
     5041 tatgcggaga ggacgagcgg caggccgcca atatgatcct tgtcaggatg cgtctccatg
     5101 acgacgtcga tcgaatggtc gtagaacggc atctcttcgc cgaggcgctg cagcacttcg
     5161 cgcgtgccgc cgccgtcgat aagcatctgg tttccggtcg ggctctcgat aaggatcgcg
     5221 tcgccctgcc cgatgtcgag caccgatact ttgagatcgt gcggccgttc gcggagaacc
     5281 gcgtaccata cgagggcgtt caaaagcgcg gcgaaggcaa caagcgcgag aaggatccgt
     5341 ttccgagtca tgcatttatt taagcataat tcatcgggga atcatcgcgg cggagtaatg
     5401 tggtatataa ttgatacgct taatcgctaa cctctacact atgacaaaat tcgaagagca
     5461 gaaattcaca attccggcgg aactcaaggg catctcgcag aagacaatgg aagagcacct
     5521 gaagctctac gccggctatg tcaaaaatgc aaatctcgtc caggagcaca ttgccgaact
     5581 ttcaaaagat gccgagaaaa acgcatacga gcttggagaa ttggagcggc atttctcgtt
     5641 cgagttcaac ggcatgagaa atcacgaggt ctatttcgcg agcttcgaag gcggaccgaa
     5701 ggaactttcg gcagagagcc cgctccgtgc cgccatcgcg gccgcctggg gcaacttcga
     5761 tgcatggctt gcaaaattca agtcgatcgc cgcgacgacc cgcggcatcg gctggacggc
     5821 actctggtat gatccggcaa gcaaaaatct cctgaattca tggatcgacg agcagcatct
     5881 ggggcaactt cagaattgca aaatgatcct cggcctcgac atgtgggagc actcgtacgt
     5941 cgccgactac cagccaagcg gcaagaagca atatatcgag gacttttttg ccaatatcaa
     6001 ttggagtgta attgaaaaaa attacatcga agcggcaaaa taatcgcagt ttcgtagaga
     6061 gtaatgagta gtgggtagaa aacaaaaccg caaatgcggt tttgttttat ctttctctgg
     6121 ctactctcta cccgctactt attcttcaac tatctcaaac gcgaggctcg tatcaaactt
     6181 cggcgtttcg atttcccttt tccggggttt cgcgtgccat ttccagagaa ggccggcgta
     6241 gagcgcgtac cagagggcga tgaaccatcc cgaaacgttc tcgatcgcga acgagccgaa
     6301 cggcacgtgc gccgcggcgt gcgtaacggt gaggatgtac gcaaggatgc cgtaggatgc
     6361 gaagccgggg acgagcgcgg cgaactgaga aacgtacgcg acgagagccg tcacggagac
     6421 gccgagcatt gcgaaaggaa cgagaggcag tacgaggaga ttcgcgggaa acgccaccgg
     6481 cgagacgagg ccgctcatgt agacgagaaa cggcaggacg aaaagctggg tcgaaacggt
     6541 cgtcgccaca gtatcgcgaa acccgcgctt ctccggaacg aaccggaaat agcgcatgat
     6601 aatcggcgtc acggaaatga tgccgaccgt cgcgatgaac gacagctgga atgaaggatc
     6661 gtctgttacg atccgcgggt tctggagaac catagcaagc gcggcaagaa cgagggcgcg
     6721 gagtgcgttg tactcgcgcc ggatcgcgcg ggcgaagagc gaaatgagcg ccatcacggc
     6781 cgcgcgcacg acggaagcgc cgcctccggc catcatcgag aacaggacga tcgacagagc
     6841 gcccgcgctg agtgccgcca tactcggcag gaacgagaag atccatacga cgatccgcgc
     6901 aacgatcgaa acattgtagc ccgaaagcac gaccacgtgg gaaaggccgg cgagcttgaa
     6961 atcgtcgagc gttgattgtc ccagcgactg cttcgccccg agaagaacgc cgttcgcaag
     7021 cgatccttcc ggcggcggta tgacgcggag aattttttcc tccaccgacc gcttcaccga
     7081 aatgagtccg gcgagaatag gattgccgtg cccatgcgag aggagcgagg cttccgcgaa
     7141 gctcatctgg tagcgaatgc cgtccttgcc gagataatcc gcgtagtcga attcccttcc
     7201 cgaatcggtc tcgaaatttt gcgggcgctc cagctttccc gaaatggaaa tcagatcgcc
     7261 atagcggaag ccgctcgtga tcggcacgcg cgcgaggatt ttttcatgcg tccgaatttt
     7321 ccgcgtgccg acttcagcct ccgaaatgtt cacgacaagc tgggtgtacg aatcccgctc
     7381 gtcgggatcg gcattcacca cgcccgtgag cgtcacgatt ctctccgcga ctgcggcaag
     7441 cggatcgggc tgcggccgct ccgcaatcgc cattctgcat gcgcccatgc cgaagccgaa
     7501 aagaatgatt gcgacaagag caagttgggc gggcgtctcc ttagcgaatt tcgcatatgc
     7561 gaaaatcgct ccggccgcga gaaatgcgag gagcgagaac ggaataggga gcgagagaat
     7621 tttcgcggtg atgatcccga gtgcggcgca ggcggagaag aggtagaggg tcatgaaaat
     7681 ttttaatttt caattttaca attttcactc aagaatcaat tgatcaattc ctgccgacag
     7741 ataaatttaa acgggaacag gattgtttga atattgtaaa ctgaaaattc attgaaaatt
     7801 gcaaattgtg aattggaaat tactttgaaa aagtttttat aatctccgct tcctcttccg
     7861 cgctcgttgc tttcttgaat cgggcgcgct tgcctttgac gcgagcttcg cactccctcc
     7921 aggtcgcgtg cgtcatgacg acgccttcga cttcgcttat atatgaatag gcggcgactt
     7981 tcgaacgaga acgcgatgcg gacttctgct tctttttcga ttcatcaagg gagatatttt
     8041 ttacatcaac gccatagcct tcgagcggac ccgagtagag ctcgactttt ttgccgtcag
     8101 cgaagtccga cgcgatttcg tcaacgcgct cgttgcccgc gatgccgaca tggccgccga
     8161 cgtactgcca ggccagtccg tttgaaaaat tttccgccgc atcgaggaga tctttccaga
     8221 gatcctggtt caagacttcc cttttctcct ttgtcaccca tcctctcgtc ttccatccgt
     8281 gcgaccactt ggtgacgcca ttgatgacgt atgatgagtc ggtgtggagc acgacgggcg
     8341 cgtcgtggag caatgaagcg aaagagagcg cttcaagcgc ggcgcggagc tccatgcgat
     8401 tgttcgtcgt gttctcttcg cggccgccga gctctttgac ttctgcgttg gtcgcgacga
     8461 tagcgcccca gccgcccggt cccggattgc ccttcgatgc gccgtcggtg aatatgatga
     8521 tttccttttg caacgccata aaacaagtat agcgaaaatt aacctaattt ctaattgctc
     8581 aaattaacct aaacaatgcc cagatcccga tcactaatat gtatatagtg ttgagtattt
     8641 agattttgaa tattggaatt tatttagaaa ttaggttaat tagaaatttg ttcattatat
     8701 gatgtcgaca atgcgaagcc tgagttcgat gcggccgaga aagacgctct tctcaatgtg
     8761 cgccacgagg ttcacgcgct tccccgccgc gagttcgatg ccgaagtctt cgggcttcgt
     8821 gaagaacgcg atcgctttca ccggattgcc gcgcgaattt tcaaaaatca tttcgagatg
     8881 gagattgccc ttgccgaacg tgcgcacgtt tccgatctcg acattctcga acatgaaaag
     8941 cggcttcggg ttgccgagcc cgaacggcgc gagcttctcg atctctccat atgtattcca
     9001 tgtcacgtcg tcgagtttca tcgctctgtc gatcgtacag acctcccgcg ccgagagttc
     9061 gtcgcgaaac ttggaaaatg cggaattaag cgcgtcgtcg aggagatgaa ccttctcgat
     9121 cgtcgttgag aatccgccgc tcatcttgtg ccccccgaag tcggagagcg catcggccgc
     9181 gtgttgcatg agttccacga cgtttacgct cccgccggag cggcacgagc ccttgagtcc
     9241 gtcgcctccc tcgcgccccc aaaggaagac agggcgattg tattcttcga cgagggtatt
     9301 cgccacgagc cccaggagcg cggggcgcca gtcggggttt ccaagcacga tcacgcgttt
     9361 catttcctcg ctctcgaatt tttccgcgca gtgcttcttc gcttcgcgcg agatcgccgc
     9421 caccaaagtc ttccgttcgc tgttaatcga atcgagatgc gccacgagcc gtcccgcttc
     9481 gagaacgtcg gtcgtggcga gaagctcgaa cgcgtccatc ggcaccccca tcctcgaggc
     9541 cgcattgatg cgcggcgtga tcgtgaatcc gatgtcgtcc tcggtgagat ggctgaggtc
     9601 gagccggagc tcgcgcgaga gtttcaaaag gcccactcgc ggcgtgcgcc gcaatacttt
     9661 caatccgaaa aacgcgagaa ccctgttttc accgacaagc ggcaccatgt cggaaagagt
     9721 tgcgaggccg accatgtcga gaagccactt ctcccatcct tcctttatgc cgaacctgtt
     9781 tttagccagt ctatcttttg cgaggatggc ctgcacgagc ttccatgcga cgcccgaacc
     9841 gcagagctcc ttgaaggggt acgtgcaacc ttcctgtttc ggatcgacga tcgcgaacgc
     9901 ttccggcagc ttctcacccg gaagatgatg atcggtgatg atcacgtcga cgccgagctc
     9961 gttcgcgcgc gcgacttctt tcacgtccgc gatgccgcag tcgacggtga tgatgagctt
    10021 ggcgccttcc tccgccagtt tctccacccc cgcgatgttc agaccgaaac cctcatggtg
    10081 gcggtgcgga atgtagttcg taaaattttc gtagccgatt tttgcaaaaa gatcatggag
    10141 aatgacgccg cccggaatgc cgtcggcatc gtagtcgctc cagatcgtga tgcgctctcc
    10201 ttttttgatc gcggaaagaa ttcgctccgc cgccttttcc atccccttga ggagaaacgg
    10261 atcatggatg tgaagggcgt aatccggcac gagaaacgcc gcggcgtctt cgggcgtttc
    10321 gatgccgcgc gtgcggagga gaaaaccggt gagcggagaa gcgacgccga gctccgcatg
    10381 ttccggaagg tcgcggagga tatattcgtt ttttgaggat ttccatcgct tttggccggt
    10441 acctgcggct ttagtggctt tcatgattcg tatatcttac aacaaatcaa aatccaaaaa
    10501 tcaaaagtca aaattgcaac tcaaaagtaa aaaattggga aattttggat taaaattttg
    10561 cattttgatt tttgcctttt gcattaatgc aagtgacgta taatagagtt atggaaaatt
    10621 gcatattttg taaaatagtg aggggtgaga tcccatccca tacgatctac gaaacggacg
    10681 acttcctcgc ctttctcgac atcaatccga gttcacccgg ccacgcccag gtgatcccca
    10741 agaagcacta tcgctgggtc tgggacgtgc cgaacgccgg cgattatttc gaggcggcga
    10801 aaattgtggc gctcgcggag cgccgcgttt ggaagaccga ctgggtactc tcgaaaatcg
    10861 tcggcgacga aatcgagcat gcgcatatct gggtctatcc gcaccgggac gtggccggcg
    10921 atccgagcaa tttcgtggaa aacgcgcgac tcctgcgcgc ggcgctcgaa gaaatcaaaa
    10981 tgggaaaatg aaaattccgg cacgcgatgt gccggaattt cgtcataact atgtgtgaag
    11041 gtactcgaag taaagcgcta atcccgcggc gaaaagaagc gggaacgccg ctctccatac
    11101 gttgtacatg gcgtctttaa caaatacttt tgtcctgtat ctcatgtgac actccgcttt
    11161 gagactagca cgattttgat aaaaggaaat cccggcacgc gatgtgccga gattcttttg
    11221 ataaaaaccg aggtgtcgca ttcacgctac ctggttgctc tccgccgatt gcggcaggtt
    11281 tgaattgttc tcagcttcac tctctccgga accaagaata cggcggacgg cgttcataat
    11341 tcccgtggtt tcttggattg aacgaccgcg tctgtggcaa atcttaagca cttcgtcttc
    11401 gatcaagttc cgcctttcag gagacagagg ctcacggtgt acatctggca taggtacctc
    11461 cctccaatga aggtagcaca atttcaataa aatgaaatac cggatgagct tggaggctca
    11521 tccggcatga gcaagagcga tttatcccaa agcatcgatg ccggggagcg acccgttcgc
    11581 gaggaaatcg agcattgcgc cgccgccggt cgaaacgaag gagaatttgt cttcgatatg
    11641 gagcgattgg attgccgaga gcgtgtcgcc gccgccgacg atcgactccg ccggtgattc
    11701 ggcgatcatc ttcgcgagcg cttcggtgct ctcacgaaac ccctcctcga aaacgccgag
    11761 cggtccgttc caaaggatga atttcgcatc cgctattttt tgccgcaaca gctcgagcga
    11821 tttcacgctc gcgtcgagaa tcatttcgtc atcgccgatt tccttcactg atttttccga
    11881 ttgtttcgca ctggctttga cgaccacctc catcggcacc gcgatcttct cattcgttgc
    11941 aaacgcgctc acgtcggcgt ctttggaaag cttcgacgcg ccgacattcc agcctctcgc
    12001 cttatagata tcgttcgcga gagcgccccc gataaagagc gtgtccgcct cgcccaagaa
    12061 tttcgagacg agcgggagct tggtttcgaa cttcgcgccg cccaggataa acacgaaggg
    12121 atgcggcgca ttgaaggcgc tagcgaggcg cgtgacttcg tccgtcagaa gaaagcccgc
    12181 gaagccctga atgaatttcg gaacgccgac aatcgaggcg tgcgggcggt gcgcggtcgc
    12241 gaacgcgtca tcgacataga gatcggcgaa cgacgcgagg tgcttggcga agctttcgtc
    12301 attcgccttt tctccggaat acttccgcag gttctcgagg aggatgacgc cgccttcggg
    12361 aagcacgttc acccgctcgg ctatgtcctc gggaaaaaag tccgtgatga aatcgacggg
    12421 aaaaatcttg gagagatagt cgcgaacggg agcgagcgtc gggtttccct cggtttcgat
    12481 atggctcgcg aggatgagct tggctccctt ctcgcgaagt tcgtggatag tgggaagagc
    12541 ttttttgatc ctgtaatcgt cgtcgatctt tccatcagtc acaggcacgt taaaatcgca
    12601 gcggagaaag atttttttgt ctttgatatc ttcaatctct ttgagagttt tcattgcact
    12661 gtaaattaaa ggctgttcgc aattcgcaga agctgggcga atccttcggg ctccaggctc
    12721 tggcgtccta ccaagagtcc ctcaacttcg ccttcgctca gaatgtcgcc gacgttctgg
    12781 aaggtcgccg agccaccgta aagaaccggt atgccgaagg caatatcctt gccgtagatc
    12841 ccgagaagca ccttgcggat atagaggacc gtttcgtaga gctccttgcc gccgagcgcc
    12901 gcattgtcct tgcggccgat ggcccagacc ggctcatagg cgagaatgag gcgcttcact
    12961 tgggatttcg aaacaccggc aagagaatgt tcgatctgat ttttgagaaa ttcgaggtac
    13021 tgtccttcgg tgtcgcgctc gcgctcgccg acgcagacga ttccgaccag gccttcggca
    13081 aacgcggcgg cgatcttttt cgacacttcc tcatcgctct ctcccatggc gcggcgctcc
    13141 gagtggccga taatggaata gtgcgcgccg acgtccgccg ccatgcgcgc cgatatctcg
    13201 ccggtatgcg agcccgacag ttcccaatgg atattctgca cgccgagcgc gaaatcgtcc
    13261 ttggcgattt tcttgagctc gctcaaataa agggtgggcg ggcagatgac cacgtcagta
    13321 ttcttgagtt tagctgccga cttcttgatc tgcctgaaga cttccttggc cgcctccagc
    13381 gactgcgggt tcattttcca gttgcccgcg atgaacatgc gcttcttttt tttcatcccg
    13441 ttagagatag gaaatgctcc gcatttccgt tgtaacttaa ttctaatttt ccatcacccc
    13501 aaaaagagat acccgccaat aatctcaaac tctgactgaa tgatctctaa cgggattcat
    13561 atggcaatta tatcactccg aagaaacggc cccgaagcac ccccatactc tccacagccc
    13621 gtatgaggcg ctttcggcgt tagtattaga ggtataaagg agcgatatgc aagagacgac
    13681 aagcgccgta ctctcggaaa cgttcgagac gggcgcggaa aaaaattggt ggaagaacgc
    13741 ggtgttctac gaggtctacg tcgaccgctt tgccggcacg tttgcagatt tttccaagcg
    13801 cctcgattat ctcgccaacc tcggcattac ctgcgtccat ctcctgcccc actatccctc
    13861 gcccatggtg gacgacggct atgacgtgtc ggactatagg gatgtccgtc ccgaactcgg
    13921 cacgctcggc gatttctcgc atttcgtgca ggccgcccat agccgcggca tccgcgtcat
    13981 gatcgacttc gtgctgaacc atgtttccac atcgcacccg tggttcgtcg aagcaagctc
    14041 gtcgaaaaaa aatcccaagc gcgattattt cctctggagc gaaacgggcg aggaattcgc
    14101 gacaaccccg agcccttttc ccgatctcag ggagagcaat tgggtgagga accccgcaac
    14161 aggcgattat tatttctcca ccttctatcc ggagcaggcc gacctcaact gggacaatcc
    14221 gagggtcttc gacgaaatga cgggggtgat cgacttttgg gcggcgcttg gcgtcgacgg
    14281 cttccggctc gacgcgtcgt cgcacctcat caagcgcgaa ggcacgaatt cgcacgggct
    14341 tcccgaaacg catgcgatcc tgaagcgcct cagggcgcac atcgagaagc tcggccccga
    14401 tatcgccatc ctcgccgaag tgcacgacag cctcgccgaa atgatcaggt atttcggcga
    14461 cggcgacgag tgccacatgg tctatcactt tccgatggtc gagcagatct tcctctcctt
    14521 catccgcggc acgagcgccg gcatcgaaaa gctcgcggag ctctcggcgg gcatccccga
    14581 caactgcagt tgggccctct tccttcggca ccacgacgag atttccttgg cgacgctcgc
    14641 gccgggcgtg cgcgccgaac ttctcgacta ttacgatccg gaaagaaaat ggcgtttcag
    14701 cgaaggcacg tcgctccggc tctccgacat gatgaagaac gacatcggca aaacgctcga
    14761 cgcataccgc cttctcttcg gcctcccggg cacccccttc atttactacg gcgacgagat
    14821 cggcatgaga aacgtcccgc tcgctcctgc agaaaaggac actcggaggg cgcttcgggg
    14881 aaagttcgac tgggcggcag cgctcattgc cgagcggaat cccgcctccc tcctgcgcca
    14941 agtcgcaatg ctcctcaagg cgcgcaggca catgaagccg acggaagaat cgatacccga
    15001 gcggaccgcg gaaaccgaag aaaaaatccc cgctgccgtc tgacaagccg cgttcattga
    15061 caatttaaat atgtaatgat attcatattg cggaggacag aatgtcaatc gatgagttcg
    15121 ctgcgcttct caaaaatttg ccgcgcgaca aatactttcc ttatatcaac gggcataaac
    15181 gcatcttcat cggcacgcga attgggaaca taacatttac agtgctccag ggcgcagccg
    15241 cattcatgtt gaacgaatac gagaacttta ccaagccgac aaatctccat gtactcgctt
    15301 atatgctcaa gctgtcatac gaagacacta agcttatcgc tcatgcgacg cataacgatc
    15361 ttgatcttgc cagagccgcc gtagtgcggg gaaagattct cgacgcgctt ggtttaaagg
    15421 aagagtgacc acggccccgc aatcccgaat tgcggggcct ttttcttata cattgaaaaa
    15481 gtgctggtat actttctgca tgttcaagca gcttctacag cttttcaaac cttttatcat
    15541 gccggcctgc cgcgcctatt ggtttatcgt ccggccggaa acggcgggag cgctcatcgt
    15601 gctgacgaac aacggcgaga tgcttcttct cacccacacc tatcgctatg gctggggcct
    15661 ccccggcggc ggattgaaac gcggcgaaac gcccgaggaa gcggtgcggc gcgaagtatt
    15721 tgaagagatc ggaatagcgc tttccgcggc aactcccctc ggctcgtttc tttcgactga
    15781 cgattaccac aggaacaccg tctacgcttt cgccgccgct ctcgaaaagc gggacatttc
    15841 gacggagaat ttcgaaatcg agagcgcgca gtggttcctg cccgaaaatc tcccaccgct
    15901 cggaccggtc acgaaaaaag tcgtggaact ttataaaaac tcaatatata gaaaagaaat
    15961 ctagcttgca gctagatttc ttttctatat ttctttccaa ctgatgatct gatactggtc
    16021 gctcgtgagc gggaaggaag gcggcggccc atagaggagg ttcgcgtcat agttgaggtt
    16081 cctcgtctga tatcccgtgc cgtcggtgta ggcgaagccg tagcgttcgt tcgtgccgat
    16141 catgccgtag agggtaatcg tgtcgcggac atagctcgtg ccgcactgcg acgcatagta
    16201 gaaccttccc acccggccgt tctgggcgat gatagcgccg tcgatctcga gatcgttgtc
    16261 gctcgaatag cccacgttga aattgttctg cgcgatgaga gaaaggacgt cggcaccgtc
    16321 ataattggta tagagcaggt cggaattcac ggtaatcgaa gtgcgcgtcg acggattgtc
    16381 ggggaaacgt cccgaagcga tagtaaggcg ctcgttgtcg atctgcccgt cgacccagag
    16441 attgtcctcg acaaaaatga tgccgttcga cggatacgaa tacgtgccga taagcgtctg
    16501 attctgaatc gtccaaaggc cccagccgtc ggtgcccgag gcgccagacc agttcatgca
    16561 gttcgatgtc gcccccttga ccgatgtcac cttgtagagc tggtaggtgc cgttggtttt
    16621 caaaatgatg tgatagccct gctttcctga gttggcgtag taagcgccgc cggactgcgc
    16681 gtccgccttc atcgccgaaa ggtcggtcgt gaggcccacg aaatcgaccg cgggtacggg
    16741 aaactgcctg cccgccatga agatatcgga tcgggacgga acagcagccg gagcgcgggg
    16801 atccgcgggc gatacgcagg tatgaacgcc gaaggaagaa ttgccgttgc agtcgtccga
    16861 gtcggtatcg ttgtagcttg agagggcgct cgtgatcaca ttgtgagcga cgccgtcaaa
    16921 gcggatgccg ccgttcgagt gaatcgggcc gaaaacttcc gttccttcgc cgaagcgcat
    16981 atcgtcgttc gcgaccacgg catactttgc aagcgacgga atggcgagct gggcgacgat
    17041 ggtgcgcgtg acctccgggt ttgcatccat cgctccggtc gactgaatgg taacgatggt
    17101 cgagcctgtg ggcggcggcg tgatagtgag cgtgtatgtt cccactttct ctcccgccgc
    17161 atcataaaaa tcatgcacat acgggccggc cgcaccggtg ccgtcttggt aatcgctctg
    17221 ggcatgggcg agatgccagc ggtaataatc gatgccggct tcggcgacct ggatcgcctg
    17281 ctccctgatc gtcgccacac gcgatgcctt gaccgccgct ccggcgccaa cagcgagcgc
    17341 cgacataaga agcactgcga gcgcggagaa gacaagcgtc tgcacgagga tcgtgccttt
    17401 ggctgctttt tgctttttta tgctctgcaa gttcatgctt gaataaattc tcaatttcta
    17461 atttacaatg aattttcaat ttgcaatgat tcaaacattt gaacctgcct gccggcaggc
    17521 aggaattgat tcattggatc ttgattgaaa attggaaatt gcaaattttt cataaattgt
    17581 ctttcaaatt tcgtatcgac acctcggtcg taaattcttc cgaggacgag ctccgcgcgt
    17641 cgggcttggc gtagatcgtc gccttaacga ggcggatgga cgtgatatcg accggctggg
    17701 aaagcggcga ggaggtgcct gtgtaattgg tgtcgtagta ttcgaaaatc gagctcgtgc
    17761 cgttcgagac attggaaagg agcgtcgagg ttttttccgt cgcaatattg tacgagagcg
    17821 gcgagccggt cggtatcgtt atccccttct tgaaatcggt gccggagagg aaatacctga
    17881 tgcgctcctt tttgccgtcg ttgtacatgt cgctgtagaa gatgaactcg gaggtcgacg
    17941 cttcctcgat cgggtacgag cccacggaag acggcgacgc ctcgcgcgct tcggaggtga
    18001 aggatttgag cattgcccgg gcgccgagct ggaaatcgag attgccctgg aagttgaacg
    18061 cgagcgagaa aacgtccttc tggaatgtcg acacggcaag gccgacaacg acaagaatgg
    18121 cgatggcaat gacgagttcg gcgagcgtga accctgtagt gaatttcgac tttggacttc
    18181 gtccgttccg gccgccgccg gacccgaatc gaaatctact acaggggaag cctttttgga
    18241 gcggcgtgcg gttcatgatt attcgggatt gaggctgata ttggccgacg accatgtcga
    18301 tcccacgctg tgccctatcg agtaatcctg gtagccgctc ttggtgacgg tgatggtata
    18361 cgttcccgtc tcgagattgg tgaagattac ctgcccgggc ggcgtgcagg cggtggtgaa
    18421 ggtgaacgcg acgtcggata cgttcggcgt aaccgacgta tcgtcggtcg acaggaacgc
    18481 tttgtagcgg atgtagcgat ggccgctcaa ggacggcgcg atcgtcctgt tcgagagcgt
    18541 gtagtaagtc gaggtactgc cgtcggggcc ggtgtagacc caggtcaggt cgtcgtcgtt
    18601 cgcggcaagc tggaatttca ccgcgtcggc gccggcagag ggcggttcgt ccaagggcgt
    18661 ccaggaaatc tcgttgtagt tcgtcgacga gccgacgtcg aaggtcgacg aggtgagcgt
    18721 tcccgacgat tcatattgcc cgaggcggtt cgcgagcttc aattccccct ccggctcgcc
    18781 ggtctcgacg ttgccgtcgc tcgtgctcca catcgtctcg tcggcaaaat cgttctgccc
    18841 gccgcctccc gaccaatctg tctggcggag atatccttcg cccgtcgttt cggtctcgtc
    18901 gaatccgtcc gggccggtaa tctcgaccga agcgccggaa accggaagtc cggtcccgct
    18961 gtcgacgacc gacacgagca cgctctgcgg cgttttcgag tggacgatca tctggatatc
    19021 ctggctagcc cccggaccga tcgtcaccgg attcaccgga ttcatgccga gaagctcgta
    19081 gctcgaatcg gtcatggcga aggtgtacgt atcccattcg acattgctgt cggagtatgc
    19141 gccgccggaa ttcgtcgtgg cggcgacggt atgcttcggc acgtcgggac tcgtgccgat
    19201 caccttcgag ccggcgtatg tgaagccgat attccccact gcggagcagg tcggcgtgac
    19261 agtcgtgatc ttcgcggtcg acactttgtc gatcgaaaac gaggtctgcg tcacctgctg
    19321 aaggagcacg gtagcgtcgg tgtgcgacgg cgtggacgat ccgagatcgc ccggcgcgta
    19381 cgtttggtcg gtggtgtagc cggacttcga cacgacgatg tggtacgcgt cggttccggg
    19441 cggtacgtcg acaagctcga gcattccgcc ctggtcggtc acgtcgtcga tggtgatggt
    19501 cgacgtggcc gtgtcgttca ccacatgcac gttggcgtcg gcaaccggat tgccgttgcc
    19561 gtcgaatacc ttgatgaaaa gcgcgccgtt agtggacgcc gtctcgagat ttttcggcgc
    19621 cacgtgagtc gccacggaga tcggcgtgaa attcttgcag gcctcgcagc tgatatcgag
    19681 ctggacaagc ttgtagtcgg cgggcgaagt gtcgttcggc gtaccgccga gcgtgccgtc
    19741 gaacggatcg tcgatgttgc gcacggtcgt aacgaccgtg aaatcggcgc cgtcgcggtt
    19801 gagcacctgc gaatgcgcga gcacgccgtc gggaatggaa ttctcgacgc cgacatccga
    19861 gtacggcatg ttgcggacga tctcgagctc ctcgttcgcg agcgccgcgg catccacctt
    19921 gtagcgtgac gcttcgaccg tcctatagat ggaggtatag ctctggtaga cggaaagcgc
    19981 gacgataaga aagactgccg agcccaccag aagctcgacc agggtgaacc ctgtagtgaa
    20041 tttcggcttt ggactttgtc cgttccgatc atggacggaa ccaagccgaa atctactaca
    20101 ggggaaaccg aaagaaacgc gccgcatcgc gcgtacattc aaagaaaaag agccgctctt
    20161 ccctcgaaac attcatatat tatcgcataa aaaatcccac tgctctgcgg cacgtgggaa
    20221 taaaaaccag cctaagctgg tttattactt aaaagaattt cactgtcgaa aggaataggt
    20281 tgagatattg ctgaactgtt gcttcatctt gaggagttgc ggcatatggc gtgccatcgt
    20341 atccatgact gattcctgaa tatagggaat ataacttacc attatttatt atgtggaccc
    20401 atgtggattc tgagggatga agtgtgtttc catcactaat tacctctttt agtgcctgct
    20461 tgcctgccac agtaataatc tcgctcgatt ctattactgg cgtagttcca ttggttattt
    20521 tgattgattg aatttcaggg ttatcctttg atacaccttt ctgataatca aactccagat
    20581 tactaacatc ttgcactgtc gtcgtggcaa ttcttatacc aaaacctttt ggatcagtgt
    20641 ttacaccgct gtagtagctg atctcaccaa tatctaatga agaattgtgc atattttgtc
    20701 cctcatcaat tctcacattt tctggatact cgaattcgat tccatacttg tcgctatgat
    20761 aggtcttagg tgttccggtt gttgttgagg aagtggtgtc ggatatcata ttgttattca
    20821 attttcccat ccaggagaca aggaaaagtg ccatgattgc aaagagaaaa acaattccag
    20881 caagaatgta ataggctgtt tttattttca tactgcttag tataagtaat agatataaaa
    20941 aagaaagcac ctcatccaca atgtggatga ggtgcttgat aaaaaataca aagaacaatt
    21001 actgaggttg aacttcccac agcactctaa acgtggaagg aatgccgcca ttggagtcac
    21061 cggagcctgg cccataagga tcacttggag tacacccaat ataaaaccca aaatgtaaat
    21121 gtggcggtat tatttcttga gtatcgtaat cgcttgcctg gccaattact tgccctcgtt
    21181 ttactactgc ataaccatga tcaatgatct gctgccttaa atcgaatcct gtacttggat
    21241 ggtcgagatc agacatgtgt aaatacactg agatgtatga acgccaataa ttgccagtaa
    21301 aacaacctct actggaatcg ccagtgttag gaaatgttga ggaatcacta ccccatacgc
    21361 ccttatcatg aataatatag accgcatggt atttactcca ctggtcatta ttaagatcat
    21421 gaagtttaca atttgaggga tctctccata catttgtacc gtcatagctg gtgttatctg
    21481 tcgccacaca caagactcca tattcagctg catgcacatc agctccatca atacctgaat
    21541 gtgcataatc gtaaccgcga tggccactac ctgaaccgtt ctcataaaac agaaaggtgt
    21601 gttgattcgt gttgacttca ggatgttcgt cccgatatgt caaaagtggc aatagaaatt
    21661 gtgcacctcc gtttttctta tatccatgaa ggccactatc attttgatca ccattcgaag
    21721 ctaacccctc ttctccgttg tatgccacaa tcgagttggc atcgccggtg gtagagatat
    21781 tctcatcatc gttatgatcc ataaccgcaa aaacttctgt gttatatgga gtcataagag
    21841 tactaccgaa cgaacccttg agaggaaatt gcatgtaccc tcctgtagaa tatccattca
    21901 caaaacgaat atctgaaata tcgacatttc caacacctga tgcggggctt ttgaaagcta
    21961 cccccgagac cagagtgtta tttccgtatt caccaacgcc caacatagcg aagggtattt
    22021 gggctctgca accaaagctt ggccaagtac ccggagaacc ggatccgtcc agacaatgca
    22081 tttttaccgc ttgccaaatt tcattggcag gtgatccttg atcggtaaaa atattcccgt
    22141 tctcatcacg taatgcaaca agaatatctg gactggctga tacccccata tcaaatgcaa
    22201 gggaatcata ggaatttaca ttcacatatc ctgaactctt atcaagatcc actattccgt
    22261 ttgcaacaga atatgtagca cgaatatgat aaggattaat gtaaaaactg ctggatccgg
    22321 tattgtcttc gtctacgctc gcacctgatg agtttgtcga ggtccatcca ctgtataaac
    22381 tgtagtgata gatgtacaac ggatacggat cgggcatttg ttcatcataa aactgaacaa
    22441 ttgccacctg catggtcgac gccgcagaca tttcgaaact tattttcgaa atagtcatgg
    22501 gactcgaact ccaccgatac aaaggaatgt acatatccat cggcgtattc tgacgcaacg
    22561 ttgcgccaaa ataatcagtt gcatggactc cggttccgat ctcagcaccg gttccatcgt
    22621 ataaatgaat aatgatatca ccggctgatc cagacgaatc gttattagtc acatttacta
    22681 ttagctccgt gcagccgctg gtggaaaaat tggtacttgt cgtaaattca atcgtaccgt
    22741 gagccgaaga cgaggtgacc agatagtcat ataactccgg ttcttcggtt atcgtcgcac
    22801 catttgctgc agccacactc catccagagg ttaatgtgtc attcccccag acaaggtatt
    22861 cagttgcgtt tgcattactg ccgcacaaca ggcagcatgc cataataaac agaagtgata
    22921 atgtggatct cacattttct cctttttctt tcaaagatct gcatctacaa cgaaatgcag
    22981 ttttag
//
LOCUS       DASZYS010000020         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1048420, whole genome shotgun sequence.
ACCESSION   DASZYS010000020 DASZYS010000000
VERSION     DASZYS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048420"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..656)
                     /gene="rpsJ"
                     /locus_tag="VHF05_01270"
     CDS             complement(294..656)
                     /gene="rpsJ"
                     /locus_tag="VHF05_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HVS79585.1"
                     /translation="MATKETKPKKEKAAAKSGGVSRLRIRVRAYEYKILDMSVKQIID
                     TALRHDAKVLGPIPLPTDIKKYTVNRASFVYKNAREQFEMRVHKRLIDIINPTPKIME
                     ALTNLNLPSGVNVDVKIM"
     gene            complement(689..1888)
                     /gene="tuf"
                     /locus_tag="VHF05_01275"
     CDS             complement(689..1888)
                     /gene="tuf"
                     /locus_tag="VHF05_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HVS79586.1"
                     /translation="MADFDRSKPHVNIGTIGHVDHGKTTLTAAILHTLDMNKDKGYGA
                     RVEEIDKIDNAPEEKARGITIALHHSEYWTPNRHYAHIDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGVMPQTREHILLAHQVGVPKILVFLNKCDMVGDAELIDLVEEEVRDLL
                     TKNDFDGANAPVIRGSGLKALEAKDASDEWSQKVLELINTCDTYIPNPQRDTDKSFLM
                     PVEDIFSIEGRGTVVTGRIERGVVKVGEEIEIVGLKDTQKTTVTGIEMFNKSLQEGMA
                     GDNAGILLRGLKKEDISRGQVLAKPKTVTPHSEFEAEVYVLKKEEGGRHTPFFSGYKP
                     QFYIRTTDVTGEVTLAEGVEMVMPGDTAKFKVKLVAPVALEDNTRFAIREGGRTVGAG
                     VVTKIVA"
     gene            complement(2426..2560)
                     /locus_tag="VHF05_01280"
     CDS             complement(2426..2560)
                     /locus_tag="VHF05_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79587.1"
                     /translation="MGYMQDNPWWCVVAVLCFLGACIWARLVLKDDDAHKENPRFYDS
                     "
     gene            complement(2691..4835)
                     /gene="fusA"
                     /locus_tag="VHF05_01285"
     CDS             complement(2691..4835)
                     /gene="fusA"
                     /locus_tag="VHF05_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVS79588.1"
                     /translation="MNRDYPLEKVRNFGIIAHIDAGKTTTSERILYYTGESHKIGEVH
                     EGNTVTDWMEQERERGITITAAAITCFWNPTYMGTDTSKKVRFNIIDTPGHIDFTAEV
                     KRSLRVLDGAVVVFDGVAGVEPQSETNWRYADDGNVPRICFINKLDRMGASFDRSFQS
                     ILDRLNKGAVRMHIPMGLEENFSGIIDLLTMKAYYFEGTMGNTIRIEEIPAEYQDEAK
                     KQRADLVERIVAEDEKLMNDYLEGKEPSLEDLKRALRKAVIDVKIIPVYAGSSLKNKG
                     VQLLLDAVVDLLPSPLDIPPVHGIDPVSGEEITRKASDEEPFSALAFKLQTDPFVGQL
                     TFFRVYSGYIDAGTYIYNSTTGDKERLGRIVRLQADKREEVQRVFAGEIAAAVGLKSA
                     KTSHTFCDEKHPIILDPIKFVEPVVSMRIEPKTKADQEKMGIALKKLSDEDPTFKITS
                     NPDTGETIISGMGELHLDIMVDRMTREFGVETNMGQPQVAYRETIQGEAEAEHKYIKQ
                     TGGKGQYGHVKITLRPMKPLAEGEKIPKNVKRYDDFEFIDSIKGGVIPAEFIPAVEKG
                     VHEAMERGIVAGFPMVNVSCELTFGSYHDVDSSEIAYKIAGSQAFQEAAKRAKPVILE
                     PIMKVEVTVPEKFMGDVTGSLSSKRGMIEGFEDRPGGIKVIRAKVPLAEMFGYTTQLR
                     SMTEGRGNAPMEFDHYEVVPPNVEKTIAESRK"
     gene            4992..5309
                     /locus_tag="VHF05_01290"
     CDS             4992..5309
                     /locus_tag="VHF05_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79589.1"
                     /translation="MDTITFERKEKVLLEQRAESFWEREIEVPAKCGTVRILLFVRRD
                     EQNEETVGVRIPYENIAFYQIEALFPLPAWMNGPVRLYGVFRENEGYAVKMFEIRKEG
                     TGA"
     gene            5380..>5743
                     /locus_tag="VHF05_01295"
     CDS             5380..>5743
                     /locus_tag="VHF05_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79590.1"
                     /translation="MALLQLIRHTVHGKYGAEDFMIQNDPSSVIRITFNRDEECISRI
                     DAIFGYVGHGAKTAYEITCIGGSRTASFRLKEFPGLRFHVYPAIRQPAVHEMRILPKQ
                     ETDSDTLLKGAYMVQTSAI"
BASE COUNT         1240 a         1539 c         1603 g         1361 t
ORIGIN      
        1 tattcggcgt tctcctgttt ggcggccgtt cgcacatatc gaggagatag gcctagatta
       61 ttcagggagt tttccttcgc aggaagctcc tttattttta tatgcaaaac attaaaccag
      121 aaagaaaaca cccgcgcgag gcgggtgttt tctttctgtg cgggtcttga ggtcgtggtg
      181 ttggatgcag ttcaaggcgc gaagcgaggc ttgcgaccaa aatgtacagt gagtacattg
      241 cgggagcaaa cgagcgagca acgcagaaat gcgccaacaa cgcgagatca agactacatg
      301 atcttcacat ccacgtttac accagagggg agattgaggt tcgtgagagc ctccatgatc
      361 ttcggggttg gattgatgat atcgatgagc ctcttgtgaa ccctcatctc aaactgttct
      421 cgcgcatttt tgtagacgaa ggaggcgcgg ttcaccgtgt acttcttgat gtcagtcgga
      481 agaggaatgg ggccgagcac tttcgcgtca tggcggaggg ccgtgtcgat gatctgcttc
      541 accgacatgt cgagtatctt gtactcgtat gccctcaccc tgatgcggag cctcgagact
      601 cctccgctct tcgctgccgc cttttctttt ttcggtttgg tttctttggt agccatggta
      661 gttgccgctt gcgcggcagg agcgcgcgct aggcgacgat cttcgtaacg actcctgcgc
      721 cgaccgtcct gcctccttcg cggatggcga agcgggtgtt gtcttcaaga gcgaccggag
      781 cgacgagctt caccttgaac ttcgcggtgt cgcccggcat gaccatttcc acaccctcgg
      841 cgagagtgac ttcgccggta acgtcggtcg tgcggatata gaactgcggc ttgtagcccg
      901 agaagaacgg agtgtggcgg ccgccttcct ccttcttcaa gacgtacacc tcagcctcga
      961 attcgctgtg cggggtgacg gtcttcggct tagcgaggac ctggccgcgg ctgatgtcct
     1021 ccttcttgag tccgcggaga aggatgcctg cgttgtcgcc ggccataccc tcctgaaggg
     1081 acttgttgaa catttcgatg ccggtaacgg tggtcttctg agtgtccttc aagccgacga
     1141 tttcgatttc ctcgccgacc ttcacgacgc cgcgctcgat gcggccggta acgaccgtgc
     1201 cgcggccttc gatcgagaag atgtcttcga caggcatgag gaacgactta tcggtgtcgc
     1261 gctggggatt cgggatgtag gtatcgcagg tgttgatgag ctcgagaacc ttctggctcc
     1321 actcatcgga tgcatccttg gcctcgagag ccttaaggcc cgagccgcgg ataacgggag
     1381 cgttcgctcc gtcgaagtcg ttcttcgtga gaagatcgcg gacttcctcc tcgacgaggt
     1441 cgatgagctc ggcgtcgccg accatgtcgc acttgttgag gaatacgagg atcttcggaa
     1501 cgccgacctg gtgagcgaga aggatgtgct cgcgggtctg gggcataacg ccgtcagtcg
     1561 cggcgacaac caagatcgcg ccgtccatct gagcggcacc ggtgatcatg ttcttgatgt
     1621 agtctgcgtg gccaggagcg tcgatgtggg cgtagtgcct gttcggcgtc cagtactcag
     1681 agtggtggag ggcgatggtg atgcctctcg ccttctcttc gggagcgttg tcaatcttgt
     1741 caatttcctc gacgcgggcg ccgtatccct tgtccttgtt catgtccaag gtgtggagga
     1801 tggctgcggt caaggtcgtc ttgccgtggt caacgtggcc gatagtgccg atgttgacgt
     1861 gcggcttgct gcgatcgaaa tctgccataa ttcttgtgta attaattttc gaagcttggc
     1921 gcgcgagaac ctgtgcctag cttcgaaaac tattgtgtta ataaactatt aataactgtt
     1981 cgtgagctgg atttatcgaa cgctcggccg agcgcccata tacaaaaaca gcgtcaaacg
     2041 cgtaaccaca atcttaaaca atgggcctcc aatagtcaag ataatgcaag gcggaatgct
     2101 ttcttcataa aaagtcccgc cggaagccgg agcttcaggc gggacacgga tgatcggaca
     2161 gatatatcac acttcgatgt tctcgacgga tgaggatcta gcacgaccgt ttttaactat
     2221 tttcttaagc cagagccatg taggcttcac cgacagagga gctaagagca cggccaacat
     2281 tatcaaaaga gtgccaagaa gcccgggatc cggaaattta agcatccaca ccgcctttcc
     2341 ttcatgtata taactaggaa accactgaag tcgtcaatag ccgaaatgaa cagcctgccg
     2401 attccgtaaa atcggcaggc cttgctcatg aatcataaaa tcgaggattt tccttgtgcg
     2461 cgtcgtcgtc cttaagcacg agccgcgccc agatgcacgc tcccaaaaaa caaagcacgg
     2521 caactacgca ccaccacggg ttgtcctgca tgtagcccat acgaaacctc ctgctttaat
     2581 tttcttccag gatgagcttc attcaatacg gatattcaca gacacgaaag cccccgcagg
     2641 ccgtacggcc tgcggggact tgttattagt tttgagtttc ggattccctt ttacttgcga
     2701 ctctcggcga ttgttttctc aacattgggt ggaaccactt cataatgatc gaattccatc
     2761 ggcgcgttgc cgcggccttc ggtcatcgag cggagctggg tggtgtagcc gaacatttcg
     2821 gcaagaggca ccttggcgcg gatcaccttg atgccgcccg ggcgatcctc gaagccttcg
     2881 atcatgccgc gcttggatga gagcgacccg gtaacgtctc ccatgaattt ctctgggacg
     2941 gtaacctcga ctttcatgat cggttcgaga atgaccggct tcgcgcgctt cgccgcctcc
     3001 tggaaagcct gagaaccggc gattttgtat gcgatttcgg acgagtcgac gtcgtggtag
     3061 gagccgaagg tgagctcgca ggaaacattc accatgggaa agcccgcaac gatgccgcgc
     3121 tccatagctt catggactcc cttctcgact gccggaatga attccgccgg aatgacgcct
     3181 cccttgatcg agtcgatgaa ttcgaagtca tcataccgct tcacgttctt tggaatcttc
     3241 tcgccctcgg caagcggctt catcggacga agcgtgatct tcacgtgccc gtactggccc
     3301 ttgcctcccg tctgcttgat gtatttgtgc tccgcttccg cttcgccctg gatggtctcg
     3361 cgatacgcga cctgcggctg gcccatgttg gtctcgacgc cgaattcgcg cgtcatgcgg
     3421 tcgaccatga tgtcgaggtg gagttcgccc atgcctgaga tgatcgtttc tccggtatcg
     3481 ggattggacg tgattttgaa cgtcggatcc tcgtcggaga gcttcttaag cgctatgccc
     3541 atcttctcct gatcggcctt ggtcttcggc tcgatgcgca tcgaaacgac cggctcgacg
     3601 aacttgatcg gatcgagaat gatcggatgc ttctcgtcgc agaaggtgtg cgatgtctta
     3661 gcgctcttaa gtcctactgc cgcggcgatt tctccggcaa atacgcgctg cacttcttcg
     3721 cgcttgtccg cctgaaggcg cacgatacgg ccgaggcgct ccttgtcgcc ggtggtcgag
     3781 ttgtaaatat aggttcccgc gtcaatgtag ccggagtata cacggaagaa tgtaagctgg
     3841 cccacgaacg gatcggtctg gagcttgaac gcaagcgccg aaaagggctc ttcatcgctc
     3901 gccttgcggg tgatctcctc gccggaaacc ggatcgatgc catgaaccgg aggaatatcg
     3961 agaggcgacg gaagaaggtc aacgacggcg tcgagaagaa gctggacgcc cttgttcttc
     4021 aaggaagagc cggcgtacac ggggatgatc ttcacgtcga tgaccgcctt gcggagcgcc
     4081 ctcttgaggt cctcgaggct cggctccttg ccttcgagat agtcgttcat gagcttctcg
     4141 tcctcggcga cgattctttc gacaaggtcg gcgcgctgtt tcttggcttc gtcctggtac
     4201 tcggccggaa tctcctcgat gcggatggta ttgcccatcg tgccctcgaa gtagtatgcc
     4261 ttcatcgtga ggaggtcgat gatgccggag aaattctcct caaggcccat cgggatgtgc
     4321 atgcggacgg ctcccttgtt gaggcggtcg agaatcgact ggaacgagcg gtcgaaggaa
     4381 gcgcccatgc ggtcgagctt gttgatgaag cagatgcgag gcacgttgcc gtcatcggcg
     4441 tagcgccagt tggtctccga ctgaggctcg acgcccgcga cgccgtcgaa gacgacgacc
     4501 gcgccgtcaa gaacgcgaag agagcgcttc acttcggcag tgaagtcgat gtggcccgga
     4561 gtgtcgatga tattgaagcg gaccttcttc gaggtgtccg tacccatgta ggtcggattc
     4621 cagaagcagg tgatggcggc cgcggtaatg gtgatgccgc gctcgcgctc ctgctccatc
     4681 cagtcggtga cggtattgcc ctcatgaact tcgccgatct tgtggctctc cccggtgtag
     4741 tagaggatgc gctccgaggt cgtcgttttg cctgcgtcga tgtgggcgat gattccgaag
     4801 tttcggactt tttctaatgg gtaatctctg ttcataacga attatttata actccataac
     4861 aaaaacactc cggcagagta ttcaagtgct atgactataa tataaagcgt ttcaaaaggc
     4921 aaaagccgga cacaccgaca taattgacag caggaactgc ctcaggcacc cttagagcgg
     4981 gaggctggtt catggatact attacgttcg aaagaaaaga gaaggtttta ctcgaacagc
     5041 gagctgaaag tttctgggaa cgcgaaatcg aagttcctgc caagtgcgga actgtacgca
     5101 ttctgctgtt tgtacgacgc gatgaacaaa acgaggaaac ggtcggggtt cgcattccct
     5161 acgaaaatat cgcattttac caaatcgaag cattgttccc tctgccagca tggatgaatg
     5221 gacctgtccg gctctacggt gtcttcaggg aaaacgaagg atatgccgtc aaaatgttcg
     5281 aaatacgaaa ggaaggcacg ggcgcgtaat gtttacgcgc ccttttgcaa caagttgacg
     5341 caaaacactc cttacttcac aataacctcg gaaaagctca tggccctttt acaactcatt
     5401 cgtcatacgg tccatggaaa atacggggcc gaagatttca tgatccagaa tgacccatcg
     5461 tcggttattc gcatcacatt caacagggat gaggaatgta tatcccgcat cgacgcgata
     5521 ttcggctacg tcggacatgg agccaaaacc gcctacgaaa tcacctgcat cggaggaagc
     5581 agaacggcct ctttccgcct caaggaattt cccggcttgc gctttcatgt ttaccccgcc
     5641 atcagacaac cggcagtcca cgaaatgcga atcctgccga aacaagaaac cgattccgac
     5701 acactattga aaggagcata catggtgcaa accagcgcga tca
//
LOCUS       DASZYS010000021         6466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1243627, whole genome shotgun sequence.
ACCESSION   DASZYS010000021 DASZYS010000000
VERSION     DASZYS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243627"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            858..1949
                     /locus_tag="VHF05_01300"
     CDS             858..1949
                     /locus_tag="VHF05_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79591.1"
                     /translation="MNIFLIKFIDAPFYGLWSTLWTQYPGFMIATNGLLDVMLAGVLF
                     DFKEEKSITGKVKWLLVVGWIFMLARVSFNKTLKDITIAYFRPTTAMEHGNSVLAYEA
                     FNKLGKLTPDLKYAQETYFRNTPVMLAVGWCESSGQQFDSNGNPTSNPKSTSLGLYQV
                     LRGTSAEIGRQEYHLTQEDWEAWGEKKFGYDRTTLEGNLRSADWLYQHLGLTPWESSR
                     GCWDQVAKLQIGTDKENLAAIQSSQLFGGAAAEAYPYVHDNVPDGTIEYIVHLTAPTG
                     TWSQWYTIPRAKYSFEHTCAMEIQPQGEPAFREIDPENGGPTVRLKFGEPSPVRAKRI
                     RFASGIDKPIEVTFSYFFAPESSPCSAQR"
     gene            complement(2049..2318)
                     /locus_tag="VHF05_01305"
     CDS             complement(2049..2318)
                     /locus_tag="VHF05_01305"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79592.1"
                     /translation="MLSITPLLNAVNRYILNPIIVLMFMVALAMFFWGIFQFISNAES
                     DRGREEGKQNMLWGIVGMFIMISVYGIIRVVLATFGLPTPPYLPL"
     gene            complement(2355..2699)
                     /locus_tag="VHF05_01310"
     CDS             complement(2355..2699)
                     /locus_tag="VHF05_01310"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79593.1"
                     /translation="MKKIIAASLLFAPAFAFAQTGPQLENVQTLIQRVGQLVNLLIPI
                     VVAIALLLFFWGLARFIMAAGDEGAKEQGRNLMIWGVIAFVVMLSIWGIVTFIQSALG
                     IGGSYTIQIPQV"
     gene            complement(2909..3331)
                     /locus_tag="VHF05_01315"
     CDS             complement(2909..3331)
                     /locus_tag="VHF05_01315"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79594.1"
                     /translation="MRLFSKKNGSAAALFLMSPLLVAAQTQADIDALTADGSPLAPVA
                     KLLIAVGKVVNLLIPIVLSIALLSFFWGMAKFIGNAGSEEARTKGKRLMISSIIALTV
                     MVSIWGIVGFIQESLAIGGARNIAIPQGAIPASPAPTE"
     gene            complement(3342..3731)
                     /locus_tag="VHF05_01320"
     CDS             complement(3342..3731)
                     /locus_tag="VHF05_01320"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79595.1"
                     /translation="MKSTKYILAGSVLVSAAASAPLFAFAAFSGFKEYLTDIDYMVHT
                     LIYIAVAAAVLVFIWGLVKFIASAGNERSHESGRQLMIWGSIALFVIASVGGIIFFIQ
                     DNLGIYGDPIETPSFMSIAPSAGDTNA"
     gene            complement(3790..4158)
                     /locus_tag="VHF05_01325"
     CDS             complement(3790..4158)
                     /locus_tag="VHF05_01325"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79596.1"
                     /translation="MKYFLYKFRGLILAVIVSLAGSAPLFASAAPSTFSDFVLLIIDL
                     IKTAIPLISSLAVLVFIWGLVKFIAAAGDAKSHANGRQLMVWGSIALFVLVSIWGILS
                     FLSQQFDFGNNAGIPLLPEN"
     gene            complement(4155..4547)
                     /locus_tag="VHF05_01330"
     CDS             complement(4155..4547)
                     /locus_tag="VHF05_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVS79597.1"
                     /translation="MNRLRTIFWSALLIFVLSPRGASFAQETTSGGSGPAQPVNFPNP
                     FKAGTSLYDFIKTIVNDILLPVGGVIIVVMTIYAGFQFVTARGNESELRKAKANFFNV
                     VIGAAILLGAWTIATVIENTLNQLRSAV"
     gene            complement(4552..4986)
                     /locus_tag="VHF05_01335"
     CDS             complement(4552..4986)
                     /locus_tag="VHF05_01335"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVS79598.1"
                     /translation="MKSITRAFTYFVPTVTLIGMLITPFSAVAALPGIKCGVQGAGDN
                     CLIPCDGVDNPCDFADFEQLAMNLMYYMVYISVPLAAVAFAYAGFLYITSGGNPGQAS
                     RARKIFTNVGIGFVVILAAWLLVYTITSALLDTSNYDNPLKP"
     gene            complement(4993..6327)
                     /locus_tag="VHF05_01340"
     CDS             complement(4993..6327)
                     /locus_tag="VHF05_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVS79599.1"
                     /translation="MTKFQLILTGVFGGFLVLGIIVFAMHRGGGAADRAPVTIWGFVD
                     ENDMSKLLEDAGLQNDPSTPVTYVRKDPATFDSDFVSAIADGDAPDLVMVSQDQFYKE
                     KNRLFPIPYSSLDQRTFKDTYVEEAELFMQPDGIYAIPFVMDPYVMYWNRNIFQNAGI
                     SQPPQYWDEFYDLARTLTKKDGALNITRSAVGLGEYSNVTHAKNMLATLIIQAGSPIT
                     TADSSSGRIMSTFNDRGDNPTVPSEAAINFFTEFSDPAKDTYSWNRSLPDSKDMFISG
                     DLATYFGPASELFEIQAKNPNLNFDVTYMPQSRDAQNKMTFGDLQGLSLVKSSQHIAA
                     AFRVMTLLTSAKSLALLSQMTSLPPVRRDLLSTKPSSPYGIVFYDSAIFAKAWTDPDN
                     TATDKIFGDLIESITSGRSRPTQAITNAGLEIQQLLDANSTAPKAPSSVPTA"
BASE COUNT         1596 a         1693 c         1822 g         1355 t
ORIGIN      
        1 aaaccccaac aaccttctca accgtcagga ccttccgctt cacagcaggg ttcgaccggt
       61 gcgccaacct tagaacgttt cggttcaaga ccgagcgatc agcgcaaagg aaatcgcaga
      121 gacaagaagt aactctcgac ctttcttttt tgaaaacaag ccgccaaatc tctcgcagat
      181 tggcggcttt tctatttgac tttatataag tttagtgata taattgtacc agacccaaag
      241 gaaattgaaa atttaagaag gaggcattcg tgccaacaaa aacagagaat gtaggcgtag
      301 aagtatatgg attttggaga gcttgtgtaa agatgtggag acagatcaaa aaacgtcacc
      361 ccatctttgg gaacgttctc cggactatcg cgctcgtctt gtttgcagct ctggtagtgt
      421 tcttgttcat gcgtctcaat cgagacccac atcaggagtc gcctgagacg caggcaaaaa
      481 tggtggagat gcagaagcag atcgaccaat ttaaagccga tttaagagca tcgcagcaac
      541 gaactgctgc gaacaacgaa gcggctgcaa aattgtcgca atccgcagcc tcgcaagtga
      601 acacctctcc cccatcgaaa ccgccgtcta caactgttcc ggcatcacaa ccggtccaaa
      661 acgcggcacc atggtggaca tggagcggct ttactggcag caagacacgc agaacattga
      721 tttatgcatt ttttgcaagt cttgtcgtcc tattttggtg gggcctcaaa aggaagtggc
      781 tacagccgat actgaaaagc ctcattacgg caaaaagatt gcgtcgaatt gtgctcgtct
      841 tctttatcca ggcgctaatg aatatatttc tgataaagtt cattgacgcg cccttctacg
      901 ggctgtggtc gacgctctgg acccaatacc ctggattcat gattgccaca aacggcttgc
      961 tggatgtgat gttggcagga gtactcttcg acttcaagga agagaaatcg attaccggaa
     1021 aagtaaaatg gctcctggtg gtcggatgga tcttcatgct cgcaagggtc tcattcaaca
     1081 aaacccttaa agacatcacc attgcatact ttcgtcctac gaccgcaatg gaacatggca
     1141 attcggtgct ggcatacgaa gcattcaaca aactcggaaa gcttacgccg gatctcaagt
     1201 acgcgcagga aacgtatttc cgaaatactc ctgtcatgct cgcagtcggc tggtgcgaaa
     1261 gcagcggcca gcaattcgat tcgaacggca atccgacatc gaatccgaaa tcgacctcgc
     1321 tcggtctcta ccaggtgctt cgcggcacgt cggccgagat cggacgccag gaataccatc
     1381 ttacgcaaga agactgggaa gcctggggcg agaagaagtt cggctatgac cgcaccacac
     1441 ttgaaggcaa tctgagaagc gccgactggc tatatcagca cttgggcctc acaccgtggg
     1501 aaagctcccg cggatgctgg gaccaggttg ccaagcttca aatcggcacc gacaaagaga
     1561 atctggcggc cattcaaagc agccagctct tcggaggcgc ggcggcggaa gcatacccct
     1621 acgttcacga caacgtgccg gacggtacga tcgagtatat cgtgcacctt accgccccga
     1681 ccggtacttg gtcgcaatgg tatacgatac cgcgcgctaa gtattccttt gagcacacat
     1741 gtgcaatgga gattcagcct caaggcgaac cagcctttag agaaatcgac cctgaaaacg
     1801 gcggaccgac agtacggttg aaattcggcg aaccatcgcc cgtcagagcc aagcgaatcc
     1861 gattcgcttc cggcatcgat aagcctatag aagtaacgtt cagttatttc ttcgctcccg
     1921 agagcagtcc ctgctccgcg caaaggtagc ccgctcttta tgtcagtaca caaagccgca
     1981 caaaatttgt gcggcttttt catatacgca aaactgcctc gcataggcga ggcggttcgc
     2041 ttcgagagtc ataatggaag atatggaggg gtcggaaggc cgaaggtcgc gaggaccacc
     2101 cggatgattc cgtacaccga tatcattatg aacattccga caatacccca cagcatgttc
     2161 tgctttcctt cttccctgcc ccggtcgctc tcggcgttcg agataaactg gaagatgccc
     2221 cagaagaaca tcgccagcgc caccatgaac atgagaacga tgatcggatt caggatatac
     2281 ctgttgacgg cattgagaag cggtgtgatg gaaagcatgc tgcaagatta aggtacgctg
     2341 atatccgtta gacgctagac ctgcggaatc tggatcgtgt agcttccgcc gatgcccaag
     2401 gcgctctgga tgaaggtaac gattccccag atggagagca tcacgacgaa cgcgataacg
     2461 ccccagatca taaggttgcg gccctgttcc tttgcaccct catcgccagc cgccataatg
     2521 aagcgcgcca agccccagaa aaagagcaga agcgcgatcg cgacgacgat cgggataaga
     2581 aggttgacca gctgtccgac ccgttgaatg agggtctgaa cgttttccag ctgaggaccg
     2641 gtctgcgcga acgcaaaagc cggagcaaag agcaaggaag ctgcaattat tttcttcatt
     2701 ttctaaaaaa attattaata acgaatacta aacgaccttt caatctttcg attaaagcgg
     2761 cagggatcct acccttccca tttcgacaga gcttggacaa tacgttcgct tgggaacaca
     2821 tcatcgctcg cgtgtcgaaa caatgcttca gtaataatga taccttggcg gccttaaaag
     2881 tatactgctc ggctgtggat tacttccatt attcggtcgg ggcgggagac gccgggatcg
     2941 cgccttgcgg gatagcgatg ttccgagcgc cgccgatcgc gaggctttcc tggataaagc
     3001 ccacgattcc ccagatcgaa accatgacag tgagcgcgat gatggaggag atcatgaggc
     3061 gcttgccttt tgtccgcgcc tcctcgcttc ccgcgttgcc gataaatttt gccatgcccc
     3121 aaaagaatga aagcagggcg atcgagagca cgatggggat aagaaggttg accaccttcc
     3181 ccaccgcgat aagcagcttg gcaacgggcg cgagcggcga gccgtcggcg gtgagcgcat
     3241 cgatatccgc ctgcgtctgt gccgctacaa gaagcggcga catgagaaag agcgccgccg
     3301 cactgccatt cttttttgag aaaagcctca tgagattgga tttaagcatt agtgtcccca
     3361 gcggacggag cgatactcat aaacgaaggg gtttcgatcg gatcgccgta gatgccaaga
     3421 ttgtcctgga taaagaagat gatgccgccc accgacgcaa tgacgaagag cgcgatcgag
     3481 ccccagatca tgagctgcct gccgctctcg tgcgatcgtt cgttaccggc gcttgcgatg
     3541 aatttcacga gcccccaaat gaacaccagt accgcggcgg ccaccgcgat atagatgagc
     3601 gtatgaacca tatagtcgat atcggtcaga tattccttga agccgctaaa cgccgcgaag
     3661 gcaaagagcg gcgcggaagc cgccgccgat acgagcacgc tccccgccag aatgtatttc
     3721 gtacttttca tatgttgtat taattctaaa tcattattcg atattccgcc tcattcggcc
     3781 ctccacagct caattttccg gcagaagcgg gatgccggca ttattgccga agtcgaactg
     3841 ctgcgaaagg aatgacagga tgccccagat cgagaccagg acgaagagcg cgatcgagcc
     3901 ccacaccatg agctgcctgc cgttcgcatg ggacttcgcg tcacctgccg cggcgatgaa
     3961 tttcacaagc ccccagatga acaccaggac ggcgagcgac gagataaggg gaatcgcagt
     4021 cttgatgaga tcgatgatga ggagcacgaa atcgctgaag gtcgaaggcg ccgcggatgc
     4081 gaagagcggc gcggagcccg caagcgagac gatgaccgca agaatgaggc cgcggaattt
     4141 gtacaggaag tatttcatac ggcagagcga agctggttaa gggtgttttc gataacggta
     4201 gcgatcgtcc aagccccgag aagaattgcc gcgccgatca cgacgttgaa gaagttggct
     4261 ttcgctttcc gaagttcgga ttcatttccg cgcgctgtca cgaactggaa gccggcgtag
     4321 atcgtcatga cgacgataat gacgccgcct accggaagca ggatatcatt cacgatggtc
     4381 ttgatgaagt catagaggct cgtcccagcc ttgaaaggat tcgggaaatt gacgggctgg
     4441 gcaggaccgg agccgcccga tgtggtttcc tgagcgaagc tcgcgccgcg cggcgaaagg
     4501 acgaagatga gaagcgctga ccagaaaatt gtacggagtc tgttcatgga tttacggttt
     4561 taatggattg tcatagttgg acgtatcaag cagcgcgctc gtgatcgtat acaccaaaag
     4621 ccacgcggca agtatcacca cgaagccgat gcccacattg gtaaatatct tgcgggcgcg
     4681 gctagcctga cctggattgc cgccggaggt gatatagaga aagcccgcat acgcaaacgc
     4741 gactgccgca agcggcacgg aaatatagac catgtagtac atgagattca tcgcgagctg
     4801 ttcgaagtcg gcaaaatcgc acggattgtc gacgccatcg catggaataa ggcagttgtc
     4861 gcctgctccc tgtacgccgc acttgatgcc cggcaatgcg gccacagccg agaacggcgt
     4921 aatgagcatg ccgataagcg ttacggtcgg gacaaaatat gtgaatgcgc gagtgatgga
     4981 tttcatgtgc gtttatgccg tcggaacgga tgacggggct ttcggcgccg tgctgttcgc
     5041 atcgagaagc tgctggatct cgaggcccgc gttggtaatg gcctgggtcg gacgcgacct
     5101 gccggacgtg atcgactcga taaggtcgcc gaagatcttg tcggtcgccg tgttgtccgg
     5161 gtcggtccac gccttggcga agatggcgct atcatagaag acgattccgt acgggctcga
     5221 cggcttcgtg gaaagcaggt cgcggcgcac cggcgggagc gaggtcattt gggaaaggag
     5281 cgcgagcgac ttcgccgacg tgagaagcgt catcacccgg aaagccgcgg cgatatgctg
     5341 ggagcttttg acgagcgaga ggccctggag gtcgccgaag gtcatcttgt tctgcgcgtc
     5401 gcgcgactgg ggcatgtacg tcacgtcgaa attgagattc gggttcttcg cctggatctc
     5461 gaaaagctca gaggcggggc cgaaatacgt cgccaggtcg cccgagatga acatgtcttt
     5521 cgaatccgga agcgacctat tccagctgta ggtgtccttc gccggatcgg agaattcggt
     5581 gaagaaattg atggcggctt ccgacggcac ggtcggattg tcaccgcggt cgttgaacgt
     5641 gctcatgatg cggccgctcg aggaatcggc ggtagtgatc gggctgcccg cttggatgat
     5701 aagcgtggca agcatgttct tggcgtgcgt tacgttgctg tattcgccga gacctacggc
     5761 cgagcgggtg atgttcagcg ctccgtcttt cttagtgagg gtgcgggcaa gatcgtagaa
     5821 ctcgtcccaa tactggggcg gctgcgatat gccggcgttt tggaaaatat tgcggttcca
     5881 gtacatgacg tacggatcca tcacgaacgg aatggcgtag atgccgtcag gctgcatgaa
     5941 caattcggcc tcttcgacat aggtatcctt gaaagtgcgc tgatcgagcg acgagtacgg
     6001 aatcggaaac aggcggtttt tctccttgta aaattgatcc tggctcacca tcacgaggtc
     6061 gggcgcgtcg ccatcagcga tcgcggacac gaaatcgctg tcgaatgtcg ccggatcctt
     6121 gcggacatac gtgacgggag tcgacggatc gttctgaagg ccggcatcct cgaggagctt
     6181 cgacatgtcg ttctcgtcga caaagcccca gatcgtgacg ggcgccctgt ctgccgcgcc
     6241 gccgccgcgg tgcatcgcga acacgataat gccgagcacg agaaagccgc cgaacacgcc
     6301 ggtgaggatg agctggaatt ttgtcatgtc cttcagcatg cgggtggagt tatggtttcg
     6361 agaatataag cccgtaatga tgcgcgccgg cctggatctc atggtcgaac tggaagcccg
     6421 cctgggtgaa aagctcttcc gccatgacgc gggttacgac atgctc
//
LOCUS       DASZYS010000022        18190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_28279, whole genome shotgun sequence.
ACCESSION   DASZYS010000022 DASZYS010000000
VERSION     DASZYS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18190
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28279"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VHF05_01345"
     CDS             <1..804
                     /locus_tag="VHF05_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVS79600.1"
                     /translation="FSEKEPAKLPWKDLNIDVVVESTGFFTKFADARAHVAAGAKRVV
                     ISAPAKDAPKDSTEGTVLLGLNEGDLATCEVSSNASCTTNAASPLIQILHEAIGIEKA
                     VLSTTHAYTASQSIVDGPAKKDFKEGRAAAHNIVPTSTGAAIAVTKVVKDLEGKFDGI
                     SLRVPVIAGSIADVTFIAKRDTSVEEVNDILRKAAADPRWQKTFSATDEELVSSDIVG
                     SNYAAIADLGLTRVIGGNLVKVLSWYDNEWGYTNSLVEHVIMTGNHIQK"
     gene            855..1205
                     /locus_tag="VHF05_01350"
     CDS             855..1205
                     /locus_tag="VHF05_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVS79601.1"
                     /translation="MQYDLEERTDVFGESVIDFVKKLSHNDYNKPLVSQVIRSATSIG
                     ANYLEANRASSKKDFRNKISICNKESNETKHWLRMISRANPEKSDQCRILWKEAHELV
                     LIFSKIFRSSTTAH"
     gene            1254..1730
                     /locus_tag="VHF05_01355"
     CDS             1254..1730
                     /locus_tag="VHF05_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HVS79602.1"
                     /translation="MKIYFGSDHAGFEIKRALIPFVQVLGHQAEDLGDFEPDPQDDYT
                     DFVIPVARLVAHDPDNTRGIVIGGSGQGEAMAANRFHDVRAVVFNGQYAPGDGRKMPN
                     EIELSRMHNNSNVLSLGARFITEEVAKDAVKLWLATPFSNDERHVRRLRKMEEMTR"
     gene            1861..2532
                     /locus_tag="VHF05_01360"
     CDS             1861..2532
                     /locus_tag="VHF05_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79603.1"
                     /translation="MIDVVPAIIPESFSDIETAVARVAGLVDRVQIDITDGKFAPLRT
                     WPYKRTPDRDFDALLHEDEGLPYWEKVNYELDMMVMEPEEKLEDWIHAGISSAVIHLE
                     STERIEEAIEKARSFDIEVGLAVNPSTPNEALDQWMEKIDFVQFMGSDKIGFHGVELD
                     EKVLGKISSLRKKYPESIIGIDIGVNEATAPLLVDAGANRLVSGSALFDSTDIKSAID
                     YFKNL"
     gene            2600..3490
                     /locus_tag="VHF05_01365"
     CDS             2600..3490
                     /locus_tag="VHF05_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVS79604.1"
                     /translation="MLTDDQIKSLRIRANEIRTSIIEMLIAAGSGHSAGPLGMADIFT
                     ALYFGILKHDSKNPSWEDRDRLVLSNGHIAPVLYATMAYAGYFPVDELKTLRKFGSRL
                     QGHPHREWLPMLETSSGPLGSGLSQAVGMAIANRMERGNDGDRYIYCLMSDGECEEGQ
                     IWEAAMLAGKEKLRNLIAIIDRNNIQIDGFTEDIMPLEAFAAKWRAWNWNVLEIDGHN
                     FREIANAVSQAKATYEKPTMIIAHTIPGKGVSYMERDYKWHGTPPGISDMPGEPPKAD
                     QAKIALEELRTLGGRIQKGE"
     gene            3722..4702
                     /locus_tag="VHF05_01370"
     CDS             3722..4702
                     /locus_tag="VHF05_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVS79605.1"
                     /translation="MAKLNSNIFNDDVEKVPIRKGFGEGFVIAGEQDANVVGLCADLT
                     ESTQMHLFKEKFPERFVELGVAEQNLASVASGMAAMGKIPFITSYAMFSPGRNWEQIR
                     TTIAYNDRPVKIVGSHAGVSVGPDGGTHQAIEDIAITRVVPRMCVLSPCDAVEAKKAT
                     LAAAKTNTPVYIRLAREKTPVITTEETPFEIGKARIFWNTENPQAGIIATGALVHKAL
                     KVAKMLEGTGARVKVLNLATIKPLDREAVVALAKETGAIVTVEEHQVAGGMGSAVAEC
                     LSEEFPVPVEFVGVRDQFGQSGTPDELIEHYGMGEKAIAEAVRKVVQRKK"
     gene            complement(4767..5219)
                     /locus_tag="VHF05_01375"
     CDS             complement(4767..5219)
                     /locus_tag="VHF05_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79606.1"
                     /translation="MGVISFDTAEALLIQLSRKSKGEIVILLKVLAISFFLLALVPLA
                     LSHYRRASKRYIFLALLAIIAALELTRPYISMRGGAWNIPLLRLHLAFAIPSFVLLVL
                     CFALIWYRQWKARKLERDIVPISPVLSYAYIGTYSIACVLGLTLFYGH"
     gene            5246..5773
                     /locus_tag="VHF05_01380"
     CDS             5246..5773
                     /locus_tag="VHF05_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79607.1"
                     /translation="MMPVSVFPKGEFMSSFLSRREREREVLLKYYEVTHEKWSVFQDR
                     RFLAIGFIATLEVGCVTAFSFLVHTSPTTVFTKLFSIIGLVALPVLGLVLLGGLRLIE
                     GRLKDYYIEAINFGALLEERLEMPGLFVRLTHVPKTTFLELDHTEYFRLFLIGVGLFW
                     YTMLPLVLAQIIKMW"
     gene            complement(6005..7009)
                     /locus_tag="VHF05_01385"
     CDS             complement(6005..7009)
                     /locus_tag="VHF05_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HVS79608.1"
                     /translation="MEKAPLDFVSIGDITTDAFIRIKDATVNCDVNRERCKLCLDFGD
                     KIPYEFVEEARSVGNSPNAAVSATRLGLKTGLVTDVGDDHNGGECSATLQKNGVSLEF
                     VTVHKSLPTNYHYVLWYEEERTILIKQQNYPYKLPPIEPAPRWIYLSSLAENSLPYHH
                     EIAEYLKSHPETKLAFQPGTFQMKLGTEALKDLYKLSKIFFCNVEESQRILGEFETTD
                     VKVLLKKMHSLGPEIVVITDGPKGAYAYDGVDGYFMPPYPDPKPPYERTGAGDAFAST
                     FTAALALGKTIEEAIKWGPVNSMSVVQEIGAQKGLLTREKLEEYLANAPANYVPAKIM
                     "
     gene            7218..8090
                     /locus_tag="VHF05_01390"
     CDS             7218..8090
                     /locus_tag="VHF05_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HVS79609.1"
                     /translation="MTKTLRESIAEAGEKRVAIGHFNISNIEALWAVFRAAQELSVPV
                     IIGVSEGERDFVGVRQAVALVRSLREEFDYPVFINADHTYSFDRVKEAIDAGFDSVIY
                     DGAKLSVEENQKITKQCVEYARASGRDVLVEAELGYIGQSSKVWSEIPQGVSIDPATF
                     TKPEVAAAFVQATGIDLFAPSVGNIHGMMGVGKDPHLDIPLIAEIRAAAGVPMVLHGG
                     SGTPDEDFQKAIDAGMSIIHISTELRVAYRKGMERTLRENPDEVAPYRIMKKSVENMK
                     AVALERLKLFNKIS"
     gene            8109..9815
                     /locus_tag="VHF05_01395"
     CDS             8109..9815
                     /locus_tag="VHF05_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVS79610.1"
                     /translation="MENTAEITKPEPTVASVSAAVANYAVIKDDISKFFKGDIDDSAE
                     TLDKYSRDASLLEVRPKLVVFPKDSADIQALVKYVNEKKSEYPDLSITARAAGSDMSG
                     GPLNESIILEMCRYMHGFSFEGNVATVLPGTFYRDFEPETLKRGLILPSYTASKSLNT
                     VGGMVANNSAGEKTLSYGKTDAYVREIKAVFSDGNEYVIKPLSQNELEVKIRQGGFEG
                     EIYRKISLMLRQHHDEIMKAKPNVSKNSSGYALWNIEQGDLFDLTRLITGSQGTLCII
                     TEAKLELVPVKPKSKLMVIFLRDIRHVSDLVNEILPFKPESLESFDDKTMQLAIKFMP
                     SMAKKMKAGLLKLMWSFWPEALMVLTGGLPKLIILVEFGGNNDAEIDAQLDACAKKIK
                     HFGFKTRVMHNPTEAEKYWTIRRESFNLLRQHVHGRRTAPFIDDIIVRPEFMPEFVPQ
                     VTKILDDYKLVYTIAGHAGNGNFHIIPLMDMHHPENRDVIMEVSEKIYDLVVKYHGSI
                     TAEHNDGIVRTPYLLKVFGPEMVKLFKETQDIFDPQDIFNPGKKSNCTLQYFHDHIAT
                     EK"
     gene            9943..11994
                     /locus_tag="VHF05_01400"
     CDS             9943..11994
                     /locus_tag="VHF05_01400"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HVS79611.1"
                     /translation="MPFRNTAAALIVLFALAGIAGAEPVQASIAADSPGVAHFFFRQL
                     PSFDSYTNDTGNATLWSWMNEHFWRAMEYTTYFDSRTSLYSHALLYDDSYAIYKGSSW
                     QTDHPEWILTTGANGTGDKVFIDWGCSGGTCPQYAADVSNPDFRQAWIDQAKEFLARG
                     RYKGLWIDDVNMAFRFTNGSSGVTPWDPNTGAPMTEANWQKYMAEFMEEIRAQLPDIE
                     ILHNAIWYAGGGDIASRASNPYVKREEAAADLVDIERGVNDSGLTGGTGYWSYREVLN
                     YIDRIHALGKGVVLDAYAGTETDWQYSLASYYLISTGRDALGAQDMTPDNWWDGFDTD
                     LGTPSGARYDDPSGLIRRDYSGGIALVNPPDGVTRTITLPKAYDTIDSGSATTSVTLA
                     SARGIVLLTPPSPPSPPPQSSGSSGSSGSSSSHHSSTSVVEPVPPSSPQDGAPAFEIG
                     ARIRTTSNLNVRTSASVGAPTLGVAPLGVGGTVEASPIIADSYRWWEIDYDNGLSGWS
                     VGDYLTAASTPSGTSGSPASSQGGTSGLSAGARIATASTLNVRSSATLRGALLGAVPQ
                     GSGGTVVSGPSIADSYAWWEVNYDNGLDGWSAADYLLPSGAVAQSPLAKGAKVTVLDV
                     LNVRSAASTGASIAGAENTGSTGTVVSDPAAANGYTWWEINYDDGLSGWSVGNYLQAA
                     Q"
     gene            12200..12682
                     /locus_tag="VHF05_01405"
     CDS             12200..12682
                     /locus_tag="VHF05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HVS79612.1"
                     /translation="MEQEHIDRNFLLKVVQPALAGLMDGSVSTLAPIFAAAFATHDSH
                     VAFIIGVSAATGAGISMAFAEALSDTGEHTGRGHPVTRGAIVGATTFVGGILHTLPFL
                     LGNLSTALYVAYMVVGAELLAIAFIRYKYFRMNFFFSMVQVVFGGLLVLAAGLLIGSA
                     "
     gene            complement(12732..13280)
                     /locus_tag="VHF05_01410"
     CDS             complement(12732..13280)
                     /locus_tag="VHF05_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79613.1"
                     /translation="MNTTTAINGLISIEKSQISGIISPEGKMPNLEIILGVAALAAII
                     ANFFQYRSRRKLIAESRESDLREIRWRKMLVDYVEQQYGKMYGENYQISSYGIPDIRK
                     TLENNPLHSTILVREHDREYSLLEFRIEWGFDPRLTVTVDHSRVMTEKFSFENLERVF
                     SEHTIRMPLPFLIDSNVKAARR"
     gene            13284..13862
                     /locus_tag="VHF05_01415"
     CDS             13284..13862
                     /locus_tag="VHF05_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79614.1"
                     /translation="MKKLLAVSCLSLFILCAPAASAYTAHGGFEKITDHAFIYVNQAP
                     VSPLTGEEVHVNMVFKDRKFESLQNLPVHLTLVRSEYGDASKDETLESKDFVTDENGS
                     LDYHYAFPDEGYYDVVLSFAVAGASTSTEDATDFIIQARSAATSTVVSGSALPLAAYV
                     IGVALIALSIFSMAAARYRKRGAAPAEPSVDK"
     gene            14055..15284
                     /locus_tag="VHF05_01420"
     CDS             14055..15284
                     /locus_tag="VHF05_01420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVS79615.1"
                     /translation="MYTHRFGSKDSGRRSGGRFAGHGRRGPSPRFNSNTHSRGRRVGQ
                     HIDVSRFINKAAPQSERAAFAPRHLFTDFAIAPELKRNIAAKGYAQPSPIQDRAIPSV
                     LEGKDVVGIANTGTGKTAAFLIPLIEKTLKHRGEKTLIVVPTRELAIQIEQEFRGFAF
                     DLPLSAVVVVGGAPIGRQISELRRGASFVIGTPGRIKDLMERRSIDLSKFGNLVLDEA
                     DRMLDMGFINDVRYMFSKMPEKKQTLFFTATLSKEIEGLISQFLREPVRVQIENTTTP
                     ESIDQDVIRVGGADKLSVLHDLLIKPGFDKVLIFGRTKHGVEKLSRLLQQRGFKAESI
                     HGDKTHGKRQKALALFKENHVQVLIATDVAARGLDISGVSHVINYDLPATYEDYVHRI
                     GRTGRGGKRGQALTFIE"
     gene            15290..15451
                     /locus_tag="VHF05_01425"
     CDS             15290..15451
                     /locus_tag="VHF05_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79616.1"
                     /translation="MEKKPIKTFQKNGKWVEHPGATRILECACGTKYIKTRPNQESCI
                     KCMIKAAEK"
     gene            complement(15502..15819)
                     /locus_tag="VHF05_01430"
     CDS             complement(15502..15819)
                     /locus_tag="VHF05_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79617.1"
                     /translation="MSQEKGLKMLCTQNFKDRIALSYPLSEFPEIHRRMNEGDPGLIP
                     LLQCSTSISFQLTDSHGNVPEGTLDELLRFGVTQIEKLNIYMQALNILQTSLDHCKMM
                     ATM"
     gene            16099..16740
                     /locus_tag="VHF05_01435"
     CDS             16099..16740
                     /locus_tag="VHF05_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HVS79618.1"
                     /translation="MNTIHAETRDASASLKKLRSTGKIPAVFYGKKEASTPISIPAAE
                     FAKIWKEAGESSVITIERSEGTVEALIKDVDIDPVTGLPRHADFYVFEKGHALEIKVP
                     LTFEGEAPAEKEGGIVVKVLHELDIKAQPQNLPHEIIVDLSVLAKIDDQITAADLKLP
                     AGVELVQDPEDVVALATLPKEEVEEAPVDLESIEVEAKGKEAKEGEEPAVAEE"
     gene            16753..18144
                     /locus_tag="VHF05_01440"
     CDS             16753..18144
                     /locus_tag="VHF05_01440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVS79619.1"
                     /translation="MSFGQARSQELYLMHFVRNYKSYIALGLLSALLAGSLLTQPVSA
                     QVDPQREAELQAQLDEVEKEIAQQSQILAGQQQQSASYSRDISILDTQIKQAQLQIQA
                     KTIEIAKLGKDIDLKTLTINELSDKIDSGKESLSQLLRKTDQLDSSSMVEVMLSDTNL
                     SSFFEDLDSFDQIEKSMKVSFDAIEAAKSDNEEQKNTLHTQQDAATAARAEISAQQAI
                     INQKEKEKQQLLADSKNKEKTYEQTIKAKQTQAAQIRAALFALRDTQAIPFGDALNYA
                     NEASKATGVRPAFILAILQQESNLGANVGQCYLKNDTTGAGIGKNTGTPFSNVMSPTR
                     DVPPFLALAAQLGFDPHNQVVSCPQGVGWGGAMGPSQFIASTWTGLAGRIAAATGSAV
                     PNPWNARDAFFATAIFLSDLGARNGTQGEIEAAGRYYAGGNWATLGMSYAQSVQQRAQ
                     TIQTTMIDPLQGL"
BASE COUNT         4212 a         4996 c         5107 g         3875 t
ORIGIN      
        1 ttctccgaga aggagccggc gaagctcccg tggaaggatc tcaatatcga tgtggtcgtc
       61 gaatcgaccg gcttcttcac gaaattcgcc gatgcccgcg cccatgtcgc ggcgggcgcc
      121 aagcgcgtcg tcatttccgc gccggctaag gacgctccga aggattcgac cgaaggcacc
      181 gttcttctgg gactcaatga aggcgatctg gcgacctgcg aggtgagttc gaacgcctcc
      241 tgcacgacga acgcggcaag cccgctcatc cagatcctcc acgaggcgat cggcatcgaa
      301 aaagcggttc tttcgacgac gcatgcgtac accgcgtcgc aatcgatcgt cgacggcccg
      361 gcgaagaagg atttcaagga aggccgcgcc gccgcccata acatcgtgcc gacgtcgacc
      421 ggcgccgcga tcgcggtgac caaggtggtg aaggatctcg aaggcaagtt cgacggcatc
      481 tcgctccgcg tgccggtcat cgccggatcg atcgccgacg tgaccttcat cgccaagcgc
      541 gacacgagcg tcgaggaagt gaacgacatc ctccgcaagg ccgcggccga tccgcgctgg
      601 cagaagacgt tttccgcgac cgacgaggag ctggtgtcct cggatatcgt cggatcaaac
      661 tatgccgcga tcgccgatct cggcctcacc cgcgttatcg gggggaatct cgtgaaagtc
      721 ctgagctggt acgacaatga atggggatac acgaactcgc tcgtggagca cgtcattatg
      781 acaggtaatc acattcagaa ataattttga atttccaatt ttgaattttg aaaaaagagc
      841 agtaaggcaa taacatgcaa tacgatttgg aagaaagaac tgatgtgttc ggggaatctg
      901 taatagattt tgtgaagaaa ctgtctcata atgattacaa taagccactt gtatcccagg
      961 ttatacgctc ggcaacgagt attggtgcaa attatttaga agcaaacaga gccagctcaa
     1021 aaaaggattt ccgtaataag atctccattt gcaacaagga atcaaatgaa accaaacact
     1081 ggctcagaat gatttccagg gccaatcccg aaaagagcga tcagtgtcgg atattgtgga
     1141 aagaggcgca tgagttagtg cttatattca gtaaaatctt tagaagtagc acgacggcac
     1201 attaaatcat tgaagaattt aattcaaaat tttaaattca aaattcaaaa tttatgaaaa
     1261 tatacttcgg ttcagatcat gccggtttcg agatcaagag ggcgctcatt ccgttcgtgc
     1321 aggtgctggg ccatcaggcg gaagatctcg gcgatttcga acccgacccg caggacgact
     1381 acaccgactt cgtcattccc gtggcgcgcc tcgtcgccca tgatcccgac aatacccgcg
     1441 gtatcgtgat cggcggatcg ggacaagggg aggccatggc ggcgaacagg ttccatgacg
     1501 tccgcgcggt ggtattcaac ggacagtacg cgccgggtga cggcaggaaa atgcccaatg
     1561 agatcgagct ttcccgcatg cacaacaatt cgaacgtcct ctctctggga gctcgcttca
     1621 tcaccgagga agtggcgaag gacgcggtga agctctggct cgcgacgccg ttctcgaacg
     1681 acgaacggca tgtccgccgc ctccgaaaga tggaggaaat gacgagataa aattaatttt
     1741 caatttctaa tttccaatga attttcaatg tcacaatgat tcagacattt gaacctgcct
     1801 gccggcaggc aggaattgat tcattgaccc ttgattaaaa attgtaaatt aaaaatttcc
     1861 atgattgatg tagttcccgc aatcatcccc gaaagcttct cggatatcga gactgccgtc
     1921 gcgcgcgtcg cagggcttgt cgatcgcgtg cagatcgata ttaccgacgg caaattcgct
     1981 ccgttgcgga cttggccgta caagaggacg cccgatcgcg acttcgacgc attgcttcac
     2041 gaggacgaag ggctgcccta ctgggagaag gtaaactacg agctcgacat gatggtgatg
     2101 gagcccgagg aaaagctcga ggattggatc catgccggca tttcgtcggc ggtcatccat
     2161 cttgaaagca cggagcgcat cgaagaggcg atcgagaagg cccgttcctt cgatatcgag
     2221 gtcggattgg cggtcaatcc ttcgacgccg aatgaggcgc tcgatcaatg gatggagaaa
     2281 atagatttcg tccagttcat ggggagcgat aagatcggct tccatggcgt ggagctcgac
     2341 gagaaggttc tcggcaagat ttcctcattg cggaagaagt atccggaaag tataattgga
     2401 atcgatatcg gcgtgaacga ggcgacggcg ccgcttctcg tcgacgcggg cgcgaaccgg
     2461 ctcgtttccg gctccgcact cttcgattcg accgatatca aatcggcaat cgattatttc
     2521 aagaaccttt gatatacgcc aatgcttacc ccgcacgaaa gcccaaggcg gcttcgtacg
     2581 gggcaggccg acaacaagca tgttaacaga tgatcaaata aaatccttgc gcataagagc
     2641 gaatgagatt cgcacctcga tcatcgaaat gctcattgcc gccggttcgg gccacagcgc
     2701 ggggccgctt ggaatggcag atatctttac cgccctctat tttggtatct taaagcacga
     2761 ttcgaagaat ccttcgtggg aggaccgcga ccggctcgta ctctcgaacg gccatatcgc
     2821 gccggtgctc tatgcgacaa tggcatatgc cgggtatttt cccgtagatg aattgaagac
     2881 gcttcgcaag ttcggctcgc gccttcaggg ccatccgcac cgcgaatggc tgccgatgct
     2941 cgagacgagt tcggggccgc tcggctccgg cctctcgcag gctgtcggga tggcgatcgc
     3001 aaacagaatg gagcgcggca atgacggcga ccgttatatt tattgcctca tgtcggacgg
     3061 cgagtgcgaa gaaggccaga tctgggaggc ggcgatgctt gcaggaaaag aaaagctccg
     3121 caatcttatt gctatcatcg accgcaacaa tatccagatc gacggattca ctgaggatat
     3181 catgccgctc gaggctttcg cggcgaagtg gcgcgcgtgg aattggaacg tgttggaaat
     3241 cgacgggcat aatttccgcg agatcgcgaa tgccgtgagc caagcgaagg cgacatatga
     3301 gaagccgacg atgatcatcg cccacaccat tccgggcaag ggcgtgtcct acatggagcg
     3361 cgattacaaa tggcacggca cgccgcctgg gatttcagat atgcctggag agccgcccaa
     3421 ggccgatcag gcgaagatcg cgcttgagga gcttcgaacg ctcggaggaa gaattcaaaa
     3481 gggtgaataa tatgcttaat gatcctgaaa aattaaatcc gaacgtcttt aatgatgacg
     3541 tagaacaaac tccaatacgc aaaggtgaga agcgcgtatg ggaacccgca attctgattg
     3601 ggggcaagcg cttcgcaagg ccggagggcg tcggcccgag gcggggtcgc ggaatttttc
     3661 agcagaaaaa tatccgtgac ttgggaaagg ttaaccttcg acaaaccata tgttaaacga
     3721 agtggcaaaa ctgaatagta atattttcaa tgatgatgta gagaaagtgc ctattcgcaa
     3781 agggttcggc gaaggtttcg ttattgcagg cgagcaggat gcgaatgtcg taggcctctg
     3841 cgcggatctt accgagtcga cgcaaatgca tcttttcaag gagaagtttc cggagcggtt
     3901 cgtggagctt ggcgttgctg agcagaatct cgcgtcagtc gcgtcgggaa tggcggcgat
     3961 ggggaagatc ccgttcatca cgtcgtacgc tatgttcagc cccggccgca actgggagca
     4021 gatcagaacg acaatcgcct acaacgaccg gccggtgaag atcgtcggct cgcatgcggg
     4081 cgtttccgtc ggtcccgacg gcggcaccca ccaggcgatc gaagatatcg ccattacccg
     4141 cgtcgtgccg cgcatgtgcg ttttgtcgcc ctgtgacgcc gttgaggcga agaaggcgac
     4201 gctcgctgcc gcgaagacga acactccggt ctatatccgc ttggctcgcg aaaagactcc
     4261 ggtcattaca accgaggaaa ctccgtttga gatcgggaaa gcgaggattt tttggaatac
     4321 cgagaacccg caagcgggca tcatcgccac cggcgcgctc gtccacaagg cgctcaaggt
     4381 cgcgaagatg ctcgaaggca cgggcgcccg cgtgaaggtt ctcaacctcg cgactattaa
     4441 gcccttggac cgcgaagcgg tcgtcgcgct tgcgaaagaa acgggagcga tcgtcaccgt
     4501 cgaagaacac caggtcgcgg gcggaatggg aagcgccgtg gctgagtgtc tatctgaaga
     4561 atttccggta ccggtagaat tcgtcggcgt gcgcgatcag ttcggccagt cgggcactcc
     4621 cgacgagctc atcgagcact acggaatggg cgagaaggcg atcgccgaag cggtgcgcaa
     4681 ggtagttcag aggaagaaat aaattgaatc gtttgaatat gaaaagtgtc cggcatttta
     4741 aaaatgccgg acactttatt ttgagcctaa tggccgtaga acagcgtaag gccgaggacg
     4801 catgctatcg agtaggttcc aatgtaggcg taggaaagaa cgggagaaat ggggacgata
     4861 tcccgttcga gcttccgagc tttccattgt ctgtaccaaa taagcgcgaa acagaggacg
     4921 agcaacacga aggaaggtat tgcgaatgcg agatgcaagc gaaggagagg gatattccat
     4981 gctccgccgc gcatgctgat gtatggcctc gtcaattcga gcgccgcgat gatggcgagc
     5041 agggcgagaa agatgtaacg cttcgacgcc ctgcggtaat gcgacagcgc aagcggaaca
     5101 agcgcgagca gaaaaaaaga tatggcgaga actttcaaga gtatcacgat ttctcctttt
     5161 gatttccggg ataattgtat cagcaaagcc tcggcagtgt cgaacgatat gacacccata
     5221 tacaaactat aaaatatatt gtaatgtgat gccggtatcg gtttttccga aaggagaatt
     5281 tatgagttcg tttctatcgc gtcgcgagcg cgagcgcgaa gttcttttga agtactacga
     5341 agttacgcat gagaaatgga gcgtgttcca ggatcgaagg ttccttgcta ttggatttat
     5401 tgcgacgctt gaagttggct gcgtaacagc attcagcttt ctggtgcaca cgagtcctac
     5461 gacggtattt accaaattat tctcgataat cggattagtc gcacttcctg tcctcggtct
     5521 ggttctcttg ggaggcttga ggctcatcga ggggcggctc aaggattatt acatcgaagc
     5581 tatcaatttc ggcgctttat tagaagagcg gttagaaatg ccggggttgt tcgtccgtct
     5641 gacgcatgtt ccgaaaacga catttcttga acttgatcac actgaatact tccgattatt
     5701 tctgattggc gttggcctat tttggtatac aatgctgccg ttagtacttg ctcaaataat
     5761 aaaaatgtgg tagtaatagt aaaggagacg atacaatcgt gcagaaagta gtttcaaaga
     5821 aaaatctggc cttggccgct atcagcgcat ggtttcttct ggtcattttg atccttgtca
     5881 gcaagtatta cattccaccg cttcacactt tttttaaaag tttggtggga ggataaaatc
     5941 aagcggcgtt cgagaatatt ctcgaacgcc gctttcattt tcgctacttt ttacttggtt
     6001 ccgtttacat tatcttcgcc gggacgtagt tcgcgggagc gttcgcgagg tattcctcga
     6061 gcttttcgcg ggtgaggagg cctttctgcg cgccgatctc ctggacgacc gacatcgagt
     6121 tgacgggacc ccacttgata gcttcctcaa tcgtcttgcc gagggcgagc gcggcggtaa
     6181 aggtcgaagc gaacgcgtcg cccgcgccgg tgcgctcgta aggcggcttg ggatcgggat
     6241 aaggaggcat gaagtacccg tcgacgccgt cataggcgta tgctccctta ggaccgtcgg
     6301 tgatgacgac gatttcagga ccgagcgaat gcattttctt aagcaaaact ttcacgtccg
     6361 tcgtctcgaa ctcgccgagg atgcgctggg actcctcgac attgcagaag aaaatcttcg
     6421 ataatttgta aaggtccttc aatgcttcgg tgccgagctt catctggaac gttccgggct
     6481 ggaaggcgag cttcgtttcc ggatgcgatt tgagatattc ggcgatctcg tggtgatagg
     6541 gaagcgaatt ttcggcaagc gagctcagat atatccagcg gggcgcgggc tcgatcggag
     6601 gaagcttgta ggggtagttc tgctgcttga tgagaatggt gcgctcctcc tcgtaccaga
     6661 ggacgtagtg gtagttcgtc ggcaggctct tgtgcacggt gacgaattcg agggagacgc
     6721 cgtttttttg gagggtggcg ctgcattcgc cgccgttgtg gtcgtcgccg acgtcggtga
     6781 cgaggccggt cttgagcccc aaacgggttg cggaaacggc ggcgttcggg ctgttgccga
     6841 ccgatctcgc ttcctcgacg aattcatagg ggatcttgtc cccgaaatcg aggcagagct
     6901 tgcatcgctc gcgattgacg tcgcaattca ccgtcgcgtc cttgatgcgg ataaacgcgt
     6961 ccgtcgtgat atcgccgatt gatacgaaat ccaaaggtgc tttttccata tattccgtat
     7021 tgtaccacgc acggtttgaa aagtaatcgc ggcgttgcgt tttttcgcgc tcatctgtgg
     7081 taaaatttcg atatgatccc gttagagaag attcgtgtgg tttataatac taagagcgaa
     7141 tttagaatct gacggtgatt gtggaattta tattacttta ctaacggaaa tgtaaggcat
     7201 ttcctttctc taacgggatg acaaaaacac tgcgcgaaag catagccgaa gcgggcgaga
     7261 agcgcgtcgc catcggccat ttcaacattt ccaatatcga agccttgtgg gcggtcttcc
     7321 gcgccgcaca ggaactctcc gtgccggtca tcatcggcgt ctccgaaggc gagcgcgact
     7381 tcgtgggcgt acggcaggcc gtggcgctcg taaggagcct ccgcgaagaa ttcgactatc
     7441 ccgtctttat caacgccgac cacacctact cgttcgaccg cgtcaaggaa gcgatcgacg
     7501 cgggcttcga ctcggtgatc tatgacggcg cgaagctttc cgtcgaggaa aaccagaaaa
     7561 tcacgaagca gtgcgtcgag tatgcccgcg cgagcggccg cgacgtcctt gtcgaagccg
     7621 aactcggcta tatcggccag tcgtcgaagg tatggtctga gattccgcaa ggagtttcga
     7681 tcgatcccgc gacgtttacg aagccggaag tcgccgcggc gttcgttcag gcgaccggca
     7741 tcgacctctt cgcgccctcc gtcggcaata tccacggcat gatgggggtc ggcaaggatc
     7801 cgcatctcga catcccgctt attgccgaaa tacgcgcggc ggcgggcgtg ccgatggtgc
     7861 ttcacggcgg ttcgggcacg cccgacgagg atttccagaa ggcgatcgac gcgggcatgt
     7921 cgatcatcca catctccacc gagctccgcg tcgcctacag gaaggggatg gagagaacgc
     7981 ttcgggagaa tccggacgaa gtcgcgccgt acaggatcat gaaaaagagc gtcgagaata
     8041 tgaaagcggt ggcgctggag aggctgaaac tgtttaataa aatttcgtaa caaattagat
     8101 tagagcttat ggagaatact gccgaaatca cgaaacccga accgacggtt gcgtcggtta
     8161 gcgccgctgt cgcgaattat gccgttatca aggatgacat atcgaaattc ttcaagggcg
     8221 atatcgacga cagcgccgag acgctcgaca agtattcccg cgacgcgagc ctgctcgagg
     8281 tgcgcccgaa actcgtcgtc tttcccaagg acagcgccga catccaggcg ctcgtgaaat
     8341 atgtgaacga aaagaaatcc gaatacccgg atctttcgat cacggcgcgc gcggcaggct
     8401 ccgacatgtc gggcggaccg ctcaacgaat cgataattct cgagatgtgc cgctacatgc
     8461 acggcttctc gttcgaagga aacgtcgcga cggttttgcc gggtactttc taccgcgatt
     8521 tcgagccgga aaccttgaag cgcggcctca tcctgccgtc gtacaccgcc tcgaagagcc
     8581 tcaataccgt gggcggcatg gttgcgaaca attccgccgg cgaaaaaacc ctttcctacg
     8641 gcaagaccga cgcgtatgtc cgagagatca aagccgtgtt ttccgacggc aacgagtacg
     8701 ttatcaagcc gctcagccag aacgagctcg aagtgaaaat ccgccaaggc ggattcgaag
     8761 gagagattta ccgcaaaatt tccctcatgc tccgccagca ccacgacgaa atcatgaagg
     8821 cgaagccgaa cgtctcgaag aattcttccg gctacgcgct ctggaacatc gagcagggcg
     8881 acctcttcga tctcacgcgg ctcattaccg gctcccaagg cacgctctgc atcatcaccg
     8941 aagcgaagct cgagctcgtg ccggtaaagc cgaagtcgaa gctcatggtc atcttccttc
     9001 gcgacatacg gcacgtgagc gatctggtga acgagatact gccgttcaag cccgagagcc
     9061 tcgagtcgtt cgacgacaag acgatgcagc tcgcgatcaa gttcatgccg tcaatggcga
     9121 agaaaatgaa agcggggctc ttgaagctca tgtggagctt ctggccggaa gccttgatgg
     9181 tgctcaccgg cggcctgccg aagctcatca tcctcgtgga attcggcgga aacaatgacg
     9241 cggaaattga cgcgcaactc gatgcctgcg ccaagaaaat caagcacttc ggtttcaaga
     9301 cgcgcgtcat gcacaacccg accgaagccg agaaatactg gacgatccgc cgcgagagct
     9361 tcaacctcct ccgccagcat gttcacggcc gccgcaccgc gcccttcatc gacgacatta
     9421 tcgtccggcc ggaattcatg cccgagttcg tgccgcaggt taccaagatc ctcgatgatt
     9481 acaagcttgt ctacaccatc gcggggcacg cgggcaacgg caacttccac atcattccgc
     9541 tcatggacat gcatcaccct gaaaacaggg acgtgatcat ggaggtctcg gagaagatct
     9601 acgatctcgt ggtgaagtac cacggctcga tcaccgccga gcacaacgac ggcatcgtcc
     9661 gcacgccgta tcttctcaag gtgttcggtc ccgagatggt gaagctcttc aaggaaacgc
     9721 aggatatttt cgatccgcag gacattttca atcccggcaa gaaatcgaac tgcacgcttc
     9781 aatacttcca cgatcacatc gccacggaga aataaaaaaa gcgcggtggg gacaaacggc
     9841 gctttttcct tcggacggtc tgttacactt aggttaacga agttagtaat ttaggaaatg
     9901 catacgtcac ttagagccgc gtttagccgc gcctgccgaa gcatgccgtt tcggaacacc
     9961 gccgcggcac ttatcgtcct tttcgccctc gcgggtattg ccggagctga gccggtccag
    10021 gcatccattg ccgccgacag tcccggcgta gcgcatttct ttttccgcca gcttccgtcg
    10081 ttcgactctt atacaaacga caccggcaat gccacgctct ggagctggat gaacgagcac
    10141 ttctggcgcg ccatggaata cacgacatat ttcgatagca ggacgtcgct ctattcccac
    10201 gcgcttcttt acgacgattc ctacgccatc tacaagggct cgtcgtggca gaccgatcat
    10261 cccgaatgga ttctcacgac cggcgcgaac ggaacgggcg acaaggtctt catcgactgg
    10321 ggctgcagcg gcggaacgtg cccgcagtat gccgccgacg taagcaatcc ggatttccgc
    10381 caggcgtgga tcgatcaggc aaaggaattt ctcgcgcgcg ggcgctacaa aggcctttgg
    10441 atcgacgatg tcaacatggc atttcgcttc accaacggct cctcgggcgt aacgccctgg
    10501 gatccgaata ccggcgcgcc gatgacggag gcgaattggc agaaatacat ggcggaattc
    10561 atggaggaga tcagggccca gcttcccgac atagaaattt tgcataatgc aatctggtac
    10621 gcgggcgggg gagatatcgc gtcgcgcgcg tcgaacccat acgtaaagcg cgaggaagcc
    10681 gccgccgatc ttgtcgacat cgagcgcggc gtgaacgact cgggacttac cggcggcacc
    10741 ggctactggt cgtataggga agtcttaaat tacattgacc gcatccacgc cctcggcaag
    10801 ggagtcgtcc tcgacgcgta tgcggggacg gagactgatt ggcagtattc gctcgcaagc
    10861 tactacctca taagcaccgg ccgcgacgcg ctcggcgcgc aggacatgac gcccgacaat
    10921 tggtgggacg gcttcgatac cgatctggga acgcccagcg gcgcgcgcta cgacgatccg
    10981 agcggcctta tccgccgcga ttactccggc ggcatcgcgc tcgtcaatcc tcccgacggc
    11041 gtaacgagga cgatcaccct gcccaaagcg tacgacacga tcgactccgg cagtgcgacg
    11101 acgtccgtga cgctcgcctc ggctcgcggc atcgttcttc tcactccgcc ctctccgcct
    11161 tcccctccgc cgcaatcttc gggatcatcg gggtcttcag gatcatcgtc gagccatcat
    11221 agctcgacga gcgtggtgga gcccgtgccg ccttcttcgc cgcaagacgg cgctcccgca
    11281 tttgagatcg gagcgcgcat ccgcacgacg tcgaatctca atgtgcgcac cagcgcgtcg
    11341 gtcggcgcgc ctaccctcgg cgtggcgccc ttgggcgtcg gcggcaccgt ggaggcgagt
    11401 cctatcattg ccgacagcta taggtggtgg gagatcgatt atgacaacgg tctttccggt
    11461 tggagcgtcg gcgattatct tacggccgct tcaactcctt ccggcacatc cggctcgcct
    11521 gcttcttccc aaggcggaac ttcaggcctt tccgccggag cgcgcatcgc gacggcaagc
    11581 acgctcaatg tgagatcgtc ggcgactctt cgaggggcgc ttctcggcgc ggtgccgcag
    11641 ggaagcgggg gaacggtcgt gtcgggtccg agtatcgccg acagctacgc atggtgggag
    11701 gtcaattacg acaacggact cgacggctgg agcgccgcgg attacctgct tccgtcgggt
    11761 gcggtggcgc aaagcccgct cgcgaagggt gcgaaagtga cggttctcga tgtgctcaat
    11821 gtgcgctccg cggcgtcgac aggggcatcg atcgcgggcg ccgaaaatac cggaagcacc
    11881 ggcacggtcg tttccgatcc cgcggccgcg aacggctaca cctggtggga gatcaattat
    11941 gacgacggcc tttccggctg gagcgtagga aattatcttc aggcggcgca ataaataaaa
    12001 aatacggcgg cggttgacca ttttccccgc tctgatatcc ttcttcctcg tgtaatcacg
    12061 caggactatt cttatggatc ccgttagaga tccggaatgt agcgtgtcag atatcgtttc
    12121 aaatttaaga caattatgaa aataaaaatg attagccgca taactacgga aatgctcggc
    12181 atttcctatc tctaacggga tggaacagga acacatcgac aggaattttc tgctcaaggt
    12241 cgtccaaccg gcgcttgccg gtctcatgga cggatcggtc tcgacgctcg cgcccatctt
    12301 cgcggcggct ttcgcgacgc atgattcgca cgtggcgttc atcatcggcg tctcggccgc
    12361 cacgggcgcg ggcatcagca tggcgttcgc cgaagcgctc tccgacaccg gcgagcatac
    12421 ggggcgcggc cacccggtga cgcgcggcgc gatcgtcggc gccaccacgt tcgtcggcgg
    12481 cattctccac actctcccgt ttctcctcgg caacctttcg accgcgctat atgtcgcata
    12541 catggtggtc ggcgcggagc ttctggcgat cgccttcatc cgctacaaat atttcaggat
    12601 gaatttcttc ttctcgatgg tgcaggttgt cttcggaggc cttctcgtgc tggcggccgg
    12661 acttctcatc ggaagcgcat aaaagagata aaaaatgccg aggctttgac gcctcggctt
    12721 ttctcgtgtc tctaccgcct tgctgctttc acattcgaat caatgagaaa cggcaacggc
    12781 atgcgaatcg tatgctcgga aaaaactctc tccaagtttt caaacgaaaa cttttcggtc
    12841 ataaccctcg aatggtcgac ggttaccgta agccgcgggt cgaaacccca ctcgatcctg
    12901 aattccagta gcgagtactc cctgtcgtgc tccctgacaa gaatggtcga atgcaaagga
    12961 ttgttctcga gcgtttttcg tatatccgga atgccgtatg aggaaatttg ataattttcc
    13021 ccgtacattt ttccgtattg ctgttcgacg taatcgacca gcattttgcg ccatctgatt
    13081 tcccgcagat cggattcccg gctctccgcg ataagttttc tcctgcttct gtattgaaag
    13141 aagtttgcga tgatagcggc caatgccgcc acacctaaga ttatctctaa attcggcatc
    13201 ttgccctccg gggaaataat accactaatc tgcgattttt caatactgat aagcccgtta
    13261 attgccgtgg tagtattcat tatatgaaga aactccttgc cgtttcctgc ctgtcgctct
    13321 tcattctctg cgctcccgcg gcgagtgcgt acactgccca tggcggcttc gagaagataa
    13381 cggatcatgc gttcatctac gtgaaccagg cgccggtttc gccccttacc ggcgaggaag
    13441 tgcatgtgaa tatggttttc aaagatagaa aattcgagtc gcttcagaac cttcccgtgc
    13501 atctcacgct cgttcgttca gaatatggcg acgcttcgaa ggacgaaacg ctcgaatcga
    13561 aggacttcgt aaccgacgag aacggctcgc tcgattatca ctacgcgttt ccggacgaag
    13621 ggtattatga cgtcgtgctc tcctttgcgg ttgccggcgc ctcaacctcg acggaagatg
    13681 cgacggactt catcattcag gcgcgctctg ccgcgacgag taccgtcgtg agcggcagcg
    13741 cccttccgct cgcggcgtat gtcatcggcg tggcgctcat cgccctctcg atcttctcga
    13801 tggcggcggc gcggtaccgg aagaggggag ccgccccggc agaaccctca gttgacaaat
    13861 agtttatttt ttatataatg taaacgtaag taaattctct tgagttttgc ttcggttgcc
    13921 gcgcatgcgg cgctaagccc gaagacttcg agccatctaa actttcgaga tcccgccgcc
    13981 ttgcatccaa ttataaaggc gggaagctgg tgtcagctta ttgagagatt cggctcgttc
    14041 tccatttttc tgaagtgtac acgcatcgat tcgggtcgaa ggattccggg cgcaggtctg
    14101 gcggcaggtt cgccggacat ggccgccgcg gcccttcgcc gaggtttaat tccaatactc
    14161 atagcagagg ccggagagtc ggacagcata tcgacgtctc ccgcttcatt aacaaggccg
    14221 cgccccagtc ggagcgcgcc gctttcgcgc cccggcatct cttcaccgac tttgccatcg
    14281 cgcccgagct taagcgcaat attgccgcca agggatatgc tcagccgtcg cccattcagg
    14341 acagggcgat tccgtccgtg ctcgaaggaa aggacgttgt cggcattgcc aataccggca
    14401 ccggcaagac ggcggccttt ctcattccgc ttatcgaaaa aacgctcaag catcgcggcg
    14461 agaagactct catcgtcgtg ccgacccgcg aactcgccat ccagatcgag caggagttca
    14521 ggggcttcgc tttcgacctg ccgctctccg cggtagtcgt agtcggcggc gcgccgatcg
    14581 gccgccagat ctccgagctc cgccgcggag caagcttcgt tatcggcacg cccggccgca
    14641 tcaaggacct catggagcgc cgatcgatcg acctttcgaa attcggcaat ctcgttctcg
    14701 acgaggcgga caggatgctc gacatgggat tcatcaacga tgtgcgctac atgttttcca
    14761 agatgcccga aaagaagcag actctcttct tcaccgcgac gctttcgaag gagatcgaag
    14821 ggctcatttc gcagtttctt cgcgagccgg tgagagtgca gatcgagaac acaaccaccc
    14881 ctgaaagcat cgaccaggac gtcatacggg tcggaggcgc ggacaagctc tcggtcctcc
    14941 acgacctgct catcaagccc ggtttcgaca aggttctcat cttcggccgc accaagcacg
    15001 gcgtcgagaa gctctcgcgc ctcttgcagc agcgcggctt caaggccgag tcgatccatg
    15061 gcgacaagac gcatggcaag cgccagaagg cgctcgcgct cttcaaggag aatcacgtgc
    15121 aggtgctcat cgcgactgac gtcgcggcgc gcggcctcga tatctccggc gtgagccatg
    15181 tcatcaacta cgaccttccg gcgacctacg aggattacgt gcaccgcatc ggcaggaccg
    15241 gccgcggcgg caagcgcggc caggcgctca cctttatcga gtaaccaaca tggaaaagaa
    15301 gcctatcaag acatttcaga agaacggcaa gtgggtggag catcccggcg ccaccaggat
    15361 tttggagtgc gcatgcggca ccaaatacat caagacgcgg ccgaaccagg aatcgtgcat
    15421 caagtgcatg atcaaggcgg cggagaaata aaagttgcca taaaaatcag ccggagcgcg
    15481 taagcgctcc ggctgtcggt tttacatcgt tgccatcatt ttgcaatggt ccaagctcgt
    15541 ttgcaaaatg ttcagagctt gcatgtaaat gttgagtttt tcgatctgcg taactccaaa
    15601 gcggagaagc tcgtcaagcg taccttccgg cacgttgccg tggctgtccg tcaattggaa
    15661 acttatcgag gtggagcatt ggagaagcgg aataagtcca ggatctccct cattcatgcg
    15721 ccggtgaatt tccgggaatt cggatagcgg gtatgacagt gcgattctgt ctttgaagtt
    15781 ttgcgtgcag agcattttca gccctttttc ctggctcatc gatgtccact ccttaaaaag
    15841 aagataaatc tgccgccata gtacatattc caagcacctc agtcaatgga tttgtccgaa
    15901 gtctgctggc cgtcgcggac ggccagaacc cttgattttt tgaaaaggct ggttataatg
    15961 agcctcaaga atcccgttgt agaaaatggc attttaaatg atggaatgcg ggaaaaacgg
    16021 gatatagcga ttaaatataa tttttaggca agtaaataag gcctgctcca agggagcgat
    16081 gccatttttc acaacaggat gaatactata cacgcagaaa cgcgagatgc gtccgcgagc
    16141 ctcaaaaagc tccgcagcac aggcaaaata ccggccgttt tctacggcaa gaaagaggca
    16201 tccactccca tttccattcc cgcggccgag ttcgccaaga tctggaaaga ggccggagag
    16261 tcttccgtca tcactattga gcgctcggaa ggaacggttg aggcgcttat caaggacgtc
    16321 gatatcgacc ccgtcacggg acttcctcgc cacgccgact tttacgtatt cgaaaaggga
    16381 catgccctcg aaatcaaggt tccgctcacg ttcgaaggcg aggcgcctgc cgagaaggaa
    16441 ggcggcatcg tcgtgaaggt tctccacgaa ctcgacatca aggcgcagcc tcagaatctt
    16501 cctcatgaga tcattgtcga tctctccgtt ctcgcgaaga tcgacgatca gatcactgcc
    16561 gcggacttga agcttcctgc gggcgtcgag ctcgtccagg accctgaaga cgtcgtcgcg
    16621 cttgcgacac tgccgaagga ggaagtggag gaagcgcctg tcgatctcga gtctatcgag
    16681 gttgaggcca agggcaagga ggcgaaggaa ggcgaggagc ctgcagtcgc cgaggagtag
    16741 tccggatccg gaatgagttt cgggcaggcg cgttcgcagg agctctatct catgcatttt
    16801 gtacgaaact ataaaagcta tatagcattg ggccttcttt ccgcattgct tgcgggaagc
    16861 ttattgaccc aacccgtgtc cgcgcaagtc gacccccagc gcgaagcgga actccaggcc
    16921 cagctcgacg aagtcgagaa ggagatcgcg cagcagtcgc agattctcgc cggccagcag
    16981 cagcagtccg cgtcatattc gcgcgacatc tccattttgg atactcagat caagcaggcc
    17041 cagctccaga tccaggcgaa aaccattgag atcgcgaagc tcggcaagga tatcgatctc
    17101 aagaccctta ccataaacga actgagcgac aagatagatt ccggaaagga atcgctttcg
    17161 cagcttcttc gcaagaccga tcagctcgac tcgtcgtcga tggtcgaggt gatgctttcc
    17221 gataccaatc tctcgtcctt cttcgaagac ctcgattcgt tcgaccagat cgagaaatcg
    17281 atgaaggtgt cgttcgacgc gatcgaagcg gccaagagcg acaatgagga gcagaagaat
    17341 acgctccaca cccagcagga cgccgccacg gccgcccgcg ccgagatcag cgcccagcag
    17401 gcgatcatca accagaaaga aaaggaaaag cagcagcttc ttgccgattc caagaacaag
    17461 gagaagacat acgagcagac gatcaaagcg aagcagactc aggccgcgca gatccgcgcc
    17521 gcgctcttcg ccctccgcga tacgcaggcg attccgttcg gcgatgcctt gaactacgcg
    17581 aacgaggcgt cgaaggcgac cggcgtgcgc ccggcattca tcctcgcgat tctccagcag
    17641 gaatcgaacc tcggagcgaa cgtgggccag tgctatctca agaatgacac gaccggcgcg
    17701 ggcatcggca agaataccgg cacgccgttc tcgaacgtca tgagcccgac ccgcgacgtg
    17761 ccgccgttcc tcgctttggc cgcccagctc ggcttcgatc ctcacaacca ggtcgtttcc
    17821 tgtccgcagg gcgtcggctg gggaggcgcc atgggcccgt cgcaattcat cgcatcgacg
    17881 tggaccggac tcgccggacg catcgccgcg gcgaccggca gcgccgtgcc gaatccgtgg
    17941 aacgcccgcg acgcattctt cgctacggcg atcttcctct ccgatcttgg cgcccgcaac
    18001 ggcacccagg gcgagatcga ggctgccggc cgctactatg ccggaggcaa ctgggcgacg
    18061 cttggcatga gctatgcgca gagcgtacag cagagggcgc agactatcca gacgacaatg
    18121 atcgaccctt tgcaaggttt gtaatttgag cgtgcttctt tgtcgccgcc tcgcgtttgc
    18181 ttgaagcagt
//
LOCUS       DASZYS010000023         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_87416, whole genome shotgun sequence.
ACCESSION   DASZYS010000023 DASZYS010000000
VERSION     DASZYS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87416"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VHF05_01445"
     CDS             complement(<1..1101)
                     /locus_tag="VHF05_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVS79620.1"
                     /translation="MKDILKRHSKIVLAVLGVILIGGSVYASAKMLSPKAGDFQVATA
                     EEKNLTETISTEGKVTAAQNVSLSFERSGKVSYVGANVGATVKAGDVIASLDASDLNA
                     ELRQAQSALQTARLKLQSLQGAGSGAMGSDVGVSISNSLSNLKDKIVDSYVKADSAMG
                     TYVDQLFSNPQSDTPDFGITISSGSTRYSIKGTASEELNINSEKRQIDAELAAWQSAN
                     ASLADETAIMNADAKGKAALESYREFLADLAAVVNSYVPSDTSAATVYGTYQTGVAAA
                     RASVDTAISNLTSAEEQYNSSKAGASPYQISLQQAAVASAEAQVDGIQAEIAKTTLRS
                     PLDGLITTQDAKVGEVVSPGTVVASVISNGNFQ"
     gene            complement(1076..1477)
                     /locus_tag="VHF05_01450"
     CDS             complement(1076..1477)
                     /locus_tag="VHF05_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVS79621.1"
                     /translation="MSRLLRSHAGPQRPKDMQSLLQVKTLGLVAEMKNPSMKDIAEYL
                     HILSPSATDIVNRLVRDGKLERRKDKADKRIVRLHLTPSGKKMLNAGLKQMKERLQAA
                     LSVLNAEEKSELAALLEKIIDRNNEGHTEAS"
     gene            complement(1610..2203)
                     /locus_tag="VHF05_01455"
     CDS             complement(1610..2203)
                     /locus_tag="VHF05_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVS79622.1"
                     /translation="MEIEEERADTLLNADTRIFFLLNSLAGKSKAGDFLIALLAEYMA
                     YALLGGFLLALFFAPAFEYRKFQAFAIVFLAGLVSRFVFATLIRVFYKRRRPHHVHKV
                     HAVLSEKSFSFPSGHSSFFYAFSSAMYLFDPALGAWFVAATAVMTVCRVVAGVHYLTD
                     ILGGLFLGIITAILAYFFLAPHIVPHIVPLAAFALQL"
     gene            complement(2309..2548)
                     /locus_tag="VHF05_01460"
     CDS             complement(2309..2548)
                     /locus_tag="VHF05_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79623.1"
                     /translation="MLEVVQVAETPPIEVNDEQFRLYAKKGQLSIRNHSSPPPSHGQP
                     VTLVLFAQNKIGAAVMFGTVISSTQYTLEIHRPAS"
     gene            complement(2588..>3754)
                     /locus_tag="VHF05_01465"
     CDS             complement(2588..>3754)
                     /locus_tag="VHF05_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVS79624.1"
                     /translation="IITPLILTWVFQKKGVHTRMQRAEAVLAFAILIFLDYVLFWTSS
                     TQVAHISLVYFILVPLMWISLRFGARYTTLSLFITALIAFGGLLWGRMTPPSDMLGLR
                     LFQDETFIDIIAVLFFILVAIEEERKTALRNLKSHIVKLEEAVGMRDDFISIASHELK
                     TPVTTLKIYSQTMERELSQKGQETLAGQVSKMDAQLDKLISLVEDLLNVSRSRLGKLE
                     FRKEIFDLEHCIRETVADIRSTTPQRTIEIDGELSHPVYGDRDRIAQVAVNLVNNALK
                     YSPKTSPVFVRLSDEDGFVKVSVRDFGIGIDEEHHQKIFELFYRVPDAGEKTYPGFGI
                     GLHLSNNIVKRHGGKLHVKSRKGEGSEFSFTLPFAHVRRDERSRAKLQKNKLTS"
BASE COUNT          772 a          994 c         1162 g          826 t
ORIGIN      
        1 ctggaaattg ccgtttgaga tgacgctcgc gacgaccgtg cccggagaaa ccacttcgcc
       61 gaccttggcg tcctgcgtcg tgatcaagcc gtcgagcggc gagcggagcg tcgtcttggc
      121 gatctcggcc tgtatgccgt cgacctgcgc ttccgcgctt gcgacggcgg cctgttggag
      181 agaaatctgg tacgggctcg cgccggcttt cgaggagttg tactgctcct ctgccgaggt
      241 gagattcgag atcgccgtat cgacgctcgc gcgcgccgcc gcgacgccgg tctgatatgt
      301 gccgtagacg gttgcggcgg acgtgtcgct cgggacgtag gaatttacca cggcggcgag
      361 atcggcaagg aactcgcgat acgactcgag cgctgccttt cccttcgcgt cggcgttcat
      421 gatggctgtc tcgtccgcga ggctcgcatt tgcagactgc catgcggcga gctcggcgtc
      481 gatctggcgt ttttctgagt tgatgttcaa ttcttcgctc gccgttccct tgatggagta
      541 gcgggtcgag cctgagctta tcgtgatgcc gaaatcggga gtgtcgctct gcggattgga
      601 aaacagctgg tcgacgtagg tgcccatggc gctgtcggcc ttcacgtagc tgtcgacgat
      661 cttgtccttg aggttggaga gggaattcga aatggaaacg ccgacgtcgg agcccatcgc
      721 gccactgccc gcgccttgaa ggctctggag tttcaagcgg gccgtctgga gggccgattg
      781 cgcctggcgc aactctgcgt tgagatcgct cgcatcgagg gaggcgatca cgtcgccagc
      841 cttgaccgtc gcgccgacat tcgcgcccac atacgagact ttcccgcttc gctcgaacga
      901 gagcgatacg ttttgcgcgg cggtgacttt tccttccgta gaaatcgttt cggtgagatt
      961 cttctcctcg gccgtggcta cctggaaatc acccgccttc ggcgagagca tcttagcgct
     1021 cgcatatacc gatccgccga tgagaatcac gccgaggacg gcgagaacga tcttgctatg
     1081 acgcttcagt atgtccttca ttgtttctat ctatgatttt ttcgagtaat gccgccaatt
     1141 cgcttttctc ctccgcattc aggaccgaaa gggccgcttg gagccgctct ttcatctgct
     1201 tcagtccggc gttgagcatc ttcttgccgc tcggagtgag gtggagcctg acgatccgct
     1261 tgtccgcctt gtccttgcgc cgctcgagct tgccgtcgcg cacgagcctg ttcacgatgt
     1321 cggtggccga aggggaaagg atgtgcaggt attcggcgat gtccttcatc gaagggttct
     1381 tcatttccgc gacaagcccg agcgttttca cttgcaggag cgattgcatg tctttcgggc
     1441 gctggggtcc ggcgtgcgat cgcagcagcc gggacatggt gaatatcatt cctgcgagcc
     1501 tgtcatcgga tccgttgttt ctccgtgggg tctgcataaa tacttagggt tcctaaacac
     1561 ttgcgtgcct ccataatagt acaggctcgg caattgtcaa tgaaggtatt tagagctgaa
     1621 gggcgaaagc ggcaagagga actatatgag gaacgatatg gggagcgaga aagaaatagg
     1681 cgagaatcgc cgttatgatc ccgagaaaaa ggccgcccag gatgtccgtc agatagtgga
     1741 cgccggcgac gacgcggcag acggtcatga cggcagtcgc cgcgacgaac cacgctccga
     1801 gcgccgggtc gaaaagatac atggcggatg agaacgcata gaaaaacgag gaatggcccg
     1861 aagggaacga gaacgatttt tcggaaagga cggcatgaac cttatgaaca tggtgtggcc
     1921 gacggcgctt gtagaaaacc ctgatgagcg tagcaaaaac gaagcgcgag acaagacctg
     1981 cgaggaaaac tatggcaaag gcctggaatt tacggtattc gaatgcgggc gcgaaaaaga
     2041 gcgcgaggag aaagccgccg agaagcgcgt aggccatgta ttcggcgaga agcgcgatca
     2101 gaaaatcacc cgccttgctt ttgccggcga ggctgttcaa aagaaaaaag atgcgggtat
     2161 cggcattgag gagcgtatcc gccctctctt cttcgatttc catcttctaa ttctactatg
     2221 ccgacggttg cggcgcatgc caacaaaagc aaaggaaagt taaagccggc tcacatgcgt
     2281 gagccggctt attgggccgt gtccggactt aagaagccgg acgatggatt tcaagggtgt
     2341 attgggttga cgagatgacg gtgccgaaca ttacagcagc gccaattttg ttttgcgcga
     2401 acaataccaa tgttactggt tggccgtgag aaggcggcgg ggaagaatgg ttccgtatgc
     2461 ttaattgccc cttctttgca tagagcctga attgctcatc gttaacctcg atcggtggtg
     2521 tttctgccac ttgtacaacc tccaacatgc gattgtacca tttcaggcaa gcaagtcaac
     2581 ggcagagcta tgaggtcagc ttgttttttt gcagcttcgc gcgcgaccgc tcgtcgcgcc
     2641 gcacatgggc gaagggaagt gtgaagctga attccgaacc ctctcccttc cggctcttta
     2701 cgtggagctt tccgccgtgg cgcttcacga tgttgttcga gagatgaagg ccgatgccga
     2761 agcccgggta ggtcttctct ccggcatccg gcacgcggta gaagagctcg aagatcttct
     2821 gatgatgctc ctcgtcgatg ccgatgccga agtcgcggac gctcactttc acgaatccgt
     2881 cctcgtcgga gagccgcacg aagacagggc tcgtcttcgg ggaatatttg agcgcgttgt
     2941 tgacgagatt caccgcgacc tgggcaatgc ggtccctgtc gccgtagacc ggatggctga
     3001 gctcgccgtc gatctcgatg gtacgttgcg gagtggtgga gcggatgtcc gccacggttt
     3061 cccggatgca gtgctcgaga tcgaagattt ccttgcggaa ttcgagcttg ccgaggcggg
     3121 agcgggaaac gttcaggagg tcctcgacga gcgaaatgag cttgtcgagt tgggcgtcca
     3181 tcttcgacac ctggcccgcg agcgtttcct gtcctttttg cgaaagctcg cgctccattg
     3241 tctgcgaata gattttgagc gtcgtgaccg gcgtcttcaa ctcgtgcgac gcgatcgaga
     3301 tgaagtcgtc gcgcatgccg acggcttcct cgagcttcac gatgtgcgac ttgagattgc
     3361 gaagggcggt tttcctctcc tcttcgatgg cgaccaggat gaagaagagg accgcgatga
     3421 tgtcgatgaa ggtctcgtcc tggaaaagcc tgaggccgag catgtcggac ggcggcgtca
     3481 tccgccccca aagcaggccg ccgaaagcga taagagcggt tatgaaaagc gaaagggtcg
     3541 tatagcgggc gccgaaccgc agggagatcc acatcagggg cacgaggatg aaatatacga
     3601 gcgaaatatg cgcgacctgc gtcgaggatg tccagaacag gacgtagtcg aggaagataa
     3661 gtattgcgaa cgcgaggacg gcctccgccc gctgcatccg cgtgtgcact cctttcttct
     3721 ggaataccca ggtgaggatg agcggcgtga tgat
//
LOCUS       DASZYS010000024         6935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_541741, whole genome shotgun sequence.
ACCESSION   DASZYS010000024 DASZYS010000000
VERSION     DASZYS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541741"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..565
                     /locus_tag="VHF05_01470"
     CDS             44..565
                     /locus_tag="VHF05_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79625.1"
                     /translation="MLAALAALALAFLLLCLLKLGGFFRGAPYLPTNDATLERMMRAA
                     RISPGERMADLGSGDGRLVIAAALAGAEAEGYEINPLLVFLSKRAIAKRGLSERARIR
                     RTSFWKADFRNCEVVTIFGVPSIMPKLEQKLLAELPVGARVVSHSFPFPSWEGERDEN
                     VFLYIKKAPKEAS"
     gene            647..1483
                     /locus_tag="VHF05_01475"
     CDS             647..1483
                     /locus_tag="VHF05_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVS79626.1"
                     /translation="MTPFVLSTETPYFYTPISRLAAKPAIPKTFNFATYNAHFAKRPR
                     EIAEAINLCPALKECDMIALQEVEAYPDETAPRIETIARLCGYHSLYAPAREQKRRGR
                     TGTHGLAILSRHKLEDIDVFELPRYELGPKTRRRICMTCKVSLGSETVRIYNAHLDTR
                     ITAEERIAQLEPIVRDASRFPGEKIVIAGDINTIPSLKWAKDGLPIWRMGQRLIIDNY
                     MREQGFHFIEPKREDYTMRFGIKMMLDAVYVKNISILESSIEYDVKNSDHFPLRTKLR
                     LS"
     gene            1572..1742
                     /locus_tag="VHF05_01480"
     CDS             1572..1742
                     /locus_tag="VHF05_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79627.1"
                     /translation="MADQEHEAWKEKHNEKKGELKDEKADYMGHKAELHQDKADESKR
                     MADNADEEAHEG"
     gene            1845..2222
                     /locus_tag="VHF05_01485"
     CDS             1845..2222
                     /locus_tag="VHF05_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVS79628.1"
                     /translation="MADTVIYHERRERVQPLYRGTQVVWYIFSVVETILLFRFILKLI
                     GANTGAAFTQFVYAISYPFAAPFLYVVGSPRVFGSTIEWSTLIAMLVYWVIAWGIVRL
                     IVMGKPVSTEEAHRKLDAQDVEV"
     gene            2304..2918
                     /locus_tag="VHF05_01490"
     CDS             2304..2918
                     /locus_tag="VHF05_01490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVS79629.1"
                     /translation="MNETLNALVLNASLKHEDQQSNTQEAAELVLSHMKEHANIAAET
                     IRLSDKNIPVGLGFKESDDDDWPAIVEKIKAADIVIFATPIWWGNRSSLCQRIIERMD
                     AFDEEVIAGGRSALLNKVAGIVITGSEDGAQAVLAGIMEVLTFMNFTLPPNCCTYWVG
                     EVGQDSKTDPERRRANASVDHMAQNTAKNLVAVAKLLKANPLSI"
     gene            complement(2982..3464)
                     /locus_tag="VHF05_01495"
     CDS             complement(2982..3464)
                     /locus_tag="VHF05_01495"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79630.1"
                     /translation="MSLAIKILNELWNAELSYKGVKVNGFGIPTVFTGYEKKSVSTVV
                     SSLKRKGYIVRSGDGWMITQIGKKYHDRRKSFQQFDSPFAKSAPKNLIVMFDIPEAKK
                     YSREWLRDHLRKFDYKMIQKSVWVGPSPLPKKFSDYAKEIGIWDGVRTFKLARPYRNK
                     "
     gene            complement(3563..4105)
                     /gene="msrA"
                     /locus_tag="VHF05_01500"
     CDS             complement(3563..4105)
                     /gene="msrA"
                     /locus_tag="VHF05_01500"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HVS79631.1"
                     /translation="MNEVAVFGGGCFWCTEAVFKAIKGVTAVCPGYAGGTKKNPTYEE
                     VSSGNTGHAEVVEVEYDPGLVDYDTLLTIFFASHDPTSLNRQDADVGTQYRSAIFYTT
                     PEQKEKAERFINDLNASSETGRRIVTEVVPLDKFYEAEDYHKNYFANHPEAPYCQLVI
                     NPKLEKVQAKYADLLKGIKI"
     gene            complement(4261..5367)
                     /locus_tag="VHF05_01505"
     CDS             complement(4261..5367)
                     /locus_tag="VHF05_01505"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HVS79632.1"
                     /translation="MKNLKKPEVIISAVIALAIIAGAVLYFKTPQGNITAARPGGSLL
                     HDEGPAPEITSPDGYINTGGKPITFGEFKGKKVVLVDIWTYSCINCQRTLPYIKAWYD
                     KYSDQGLEIIGIHTPEFSFEKVQKNVEDAVERLGIKYPFVLDNEYGTWNAFGNQYWPR
                     KYLIDVDGNIVYDHIGEGGYEDTEKAIQEALMDRDAKLGISPADVTATSSAPENPIDA
                     EFSAIGSPETYFGSARNQYLANGKESASGIQSLILPANPELNGLYLGGNWDFFPEYAE
                     SSAGGKIVYKYDSKNVYFVASAKDPSRPVKIKVLVDGKEPGDMHGADIAPDGTGTISE
                     SRLYHLLGGENYGTHTVEIDVIDGTLDAFTFTFG"
     gene            complement(5367..6098)
                     /locus_tag="VHF05_01510"
     CDS             complement(5367..6098)
                     /locus_tag="VHF05_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HVS79633.1"
                     /translation="MLLLLITFLAGILTVITPCVLPMIPVIVGGSVAGGANRKKAYVV
                     TLSLGASVFLFTLLLKVSTLLISVPQSVWEWISGGIIALLGLSLLFPDWWARIALVGA
                     LGRKSNAIMAEGYQRHDFWGDLLTGAALGPVFSSCSPTYFVVLATVLPQNRALGIVYL
                     LSYSLGLCLTLLIVSLAGQKLLMKLGAAADPKGWLQKTVGVLLILVGIAIGTGVDRAA
                     ETNLVNNNYFDATKIEQRFLSNDQM"
     gene            complement(6285..6911)
                     /locus_tag="VHF05_01515"
     CDS             complement(6285..6911)
                     /locus_tag="VHF05_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79634.1"
                     /translation="MKAFRILLIAAVIVAVLLIAVSAYGLIQARLQANAPRDFGQVAE
                     QSANASGIFLDRDLGIAFQYGPGVETEATGTKVYVYEKGSEPASGQSVAVFKKDPKET
                     FEAAIRRIALAGNSNDCTIDIEQTNKYEGGYMTAEIVPPTAASSPSLPAWANASLCNE
                     AYAKTNGLRYFLYDTNHPGIFAFFDIGQYAIPSGEGNEPWEDTFRFAP"
BASE COUNT         1564 a         1983 c         1785 g         1603 t
ORIGIN      
        1 ctcgcgttca aaatttcgca gggattctga tttgcttaat cgcatgcttg ccgcactcgc
       61 cgccctagcg ctcgcattcc ttctcctctg cctcctcaag ctcggaggct ttttccgcgg
      121 agcgccgtat ctgccgacca atgacgcgac cctggagcgc atgatgcgcg cggcgcggat
      181 atcgccgggc gaaagaatgg cggatctcgg atcgggagac gggcggctcg tcattgccgc
      241 ggcgctcgct ggcgcggaag cagaaggata tgagatcaac cctctcctcg tattcctttc
      301 caagcgggcg atcgcgaagc gcggcctttc cgaacgcgcc cgtatccgga ggacgagttt
      361 ctggaaggct gatttccgga actgcgaggt cgtaacaatc ttcggcgtgc cgtcgattat
      421 gccgaagctc gaacagaagc tccttgccga actcccggtt ggcgcccgcg tcgtctccca
      481 ttcgtttccc ttcccttcat gggagggaga gcgtgatgaa aacgtttttc tctatatcaa
      541 aaaggctcca aaggaggctt catgaacggc attgcgaccc accaggatga tgcgatataa
      601 tggagggaaa gtgatgggac cctacgcgca atactcgcga taaatcatga ctccattcgt
      661 tctttccact gaaacgcctt atttttatac tcccatatca aggctcgcgg ccaagccagc
      721 gatcccgaaa acgttcaatt tcgccacata taacgcgcat ttcgcgaaac ggccccgcga
      781 gatcgcggaa gcgatcaact tgtgccccgc gctcaaggag tgcgacatga tcgcgcttca
      841 ggaagtcgag gcctacccgg acgaaacggc gcctcgcatc gagacgatcg ctcgcctctg
      901 cggctatcac tccctctacg cgccggcgcg ggagcaaaaa cgccgcggcc gcacgggaac
      961 gcacggactc gccatccttt cccggcacaa gctcgaagat atcgacgtgt tcgagctccc
     1021 ccgctacgag ctcggcccca agacgcgccg ccgcatctgc atgacgtgca aagtttctct
     1081 cggatccgaa acggttcgca tctacaacgc ccatctcgac acgcgcatca ccgcggaaga
     1141 gcgcatcgcc cagctcgagc cgatcgtacg cgacgcgtcg cgctttcccg gcgaaaaaat
     1201 cgtcattgcc ggcgacatca ataccattcc ctccctcaaa tgggcgaagg acggccttcc
     1261 tatctggcgc atgggacagc gactcattat cgacaactac atgcgcgagc agggcttcca
     1321 tttcatcgag cccaagcgcg aggactacac gatgcgcttc ggcatcaaga tgatgctcga
     1381 cgccgtttac gtaaagaaca tctcgattct cgaatcctcc atcgaatacg acgtgaagaa
     1441 ttccgaccat ttccccctgc ggacaaagct ccgcctctct taagtttctt caccgctttt
     1501 tcaattaaga gaggtaagct catgagtagg acggagtcgc gtcctaccaa ttttttaatc
     1561 gaaacaaata tatggcagat caagaacacg aggcatggaa ggagaagcac aatgagaaaa
     1621 aaggcgagct caaggacgag aaagccgatt acatggggca caaagccgag ctccatcagg
     1681 acaaagccga cgaatcgaag cgcatggccg acaatgccga cgaggaggcg cacgaaggct
     1741 aaagctcttc atggcacaag ccgcctttcc gggcggtttt gcttcattct ggcttaatcg
     1801 cgccggttca aaattaatac gtttataagc attaataatt ttccatggca gacacagtca
     1861 tctatcatga acgccgcgaa cgcgtacagc cgctctatag ggggacccaa gtcgtgtggt
     1921 atatcttttc tgtcgtcgaa acgatcctgc ttttccgctt tatcctgaag ctcatcggag
     1981 cgaataccgg tgcggcattc acgcagttcg tctacgccat ttcctatccc ttcgcggcgc
     2041 cgtttctcta tgtcgtgggc tcgccgcgcg ttttcggctc gacgatcgaa tggtccaccc
     2101 tcattgcgat gctcgtctac tgggtgatcg cctggggcat cgtgcggctc atcgtcatgg
     2161 gcaagcctgt ttcgaccgaa gaggcgcacc gcaagctcga cgcccaggac gtcgaggtgt
     2221 aaatgtcctc aaaaaatacc cgcttccgcg ggtatttttg tctgtccgag tggtatgatt
     2281 taagcaaaga taatgcaagc accatgaacg aaacgctcaa tgccctcgtg ctgaacgcct
     2341 ccctcaagca cgaagatcag caatcaaaca cgcaagaagc ggcggagctc gtgctctccc
     2401 atatgaaaga gcatgcgaat atcgccgccg aaacgatcag gctctcggac aagaatattc
     2461 cggtggggct cggcttcaag gagtcggacg atgacgactg gccggcgatc gtcgagaaga
     2521 tcaaggcagc cgacatcgtc attttcgcga cgcctatctg gtggggcaat cgttcgagcc
     2581 tctgtcagag aataatcgaa cgaatggacg ctttcgacga ggaggtgatc gccggaggca
     2641 ggagcgcgct tttgaacaaa gttgccggca tcgtcatcac cggaagcgag gatggcgcgc
     2701 aggcggtgct cgcgggaatc atggaagtgc tcacctttat gaacttcacc ctgccgccga
     2761 attgctgcac ctactgggtc ggcgaagtgg gtcaagactc aaaaaccgat ccggaacgcc
     2821 gccgcgcgaa cgcatcggtc gaccatatgg cgcaaaatac cgccaaaaac ctcgtcgcag
     2881 tcgccaagct tctcaaggcg aatcccctct caatctaatt caattcgcct ttgtattcac
     2941 tcaatacttt ccgcgatcgc ggaaagtatt gataattata cttacttatt tcgatatgga
     3001 cgggccaatt tgaatgttct aactccatcc cagattccaa tttctttggc ataatcacta
     3061 aatttcttgg gaagcgggct cggtcccacc cacacgcttt tttggatcat cttgtaatcg
     3121 aactttcgca gatgatctct gagccactca cgactgtatt ttttcgcttc aggaatatcg
     3181 aacatgacta tgagattttt aggagcactc ttcgcaaacg gcgagtcgaa ttgttggaat
     3241 gacttgcgcc tatcgtgata tttttttcct atttgcgtta tcatccatcc atcgccagag
     3301 cgaactatat atcctttccg tttcaaactc gaaacgacag tcgaaacgga ttttttctca
     3361 taaccagtaa atacagtggg gattccgaaa ccatttacct taactccttt atatgaaagc
     3421 tctgcattcc aaagctcgtt aagaattttt attgcaagac tcatgcttga attatatctc
     3481 acatcttgaa ctttccacat tttccgcgat cgcggaaagt atggaaagct tcattcaaaa
     3541 tatcgcaaga tgtcggatat ttttatatct ttattccctt caacagatca gcgtatttcg
     3601 cctggacctt ctcgagcttg ggattgatga cgagctggca gtacggcgct tccggatgat
     3661 tcgcgaaata attcttgtgg tagtcctccg cctcgtagaa tttatcaagc ggcacgactt
     3721 cggtaacgat ccgtcgaccc gtttccgacg atgcgttgag atcattgatg aacctctcgg
     3781 ccttttcctt ctgctcagga gtcgtataaa aaattgccga gcggtactgg gtgccgacgt
     3841 ccgcgtcctg gcggttcaag ctcgtaggat cgtgcgacgc gaagaagatc gtgaggagcg
     3901 tatcgtaatc gaccaggccc ggatcgtatt ccacctccac gacctcggca tggcccgtgt
     3961 tgccgctcga cacctcttcg tacgtcggat tcttctttgt accgcccgca tagccgggac
     4021 acacggctgt cacgcccttg atcgccttga agaccgcctc agtgcaccaa aaacaacccc
     4081 cgccaaatac ggcgacttca ttcatttcag attttgcatc gagaatattc atataaggat
     4141 aaatttagtt ggttgttttt ggaaaaagcc ctgccccgtt gcaagcgtct gcgctttgct
     4201 gcggggagcc gccgccgaaa accgcaattt cagatttttc catatgctaa ttctaatttt
     4261 ctatccgaaa gtaaacgtga aggcgtctag tgttccgtca attacgtcaa tctccacggt
     4321 atgtgtccca taattctctc cgccaagaag atggtagagc ctgctttcgc ttatcgttcc
     4381 cgtgccgtcc ggagcgatat ccgcgccgtg catatcgccg ggttccttgc cgtcgacaag
     4441 cactttgatc ttcaccggcc tcgacggatc cttcgcgctc gccacgaaat agacattctt
     4501 cgaatcgtat ttgtacacga ttttcccgcc cgcggagctt tcagcgtatt cagggaagaa
     4561 atcccaattg ccgcctagat acaacccgtt aagctcggga tttgcaggca atatgagcga
     4621 ctggatgccc gacgcactct ccttgccgtt cgcgagatat tggttgcggg ccgaaccgaa
     4681 gtacgtttcg gggctgccga tcgcgctgaa ttcggcgtcg attggatttt ccggcgccga
     4741 gctcgtggca gtgacgtcgg ccggagaaat tccgagtttc gcgtcgcgat ccatgagcgc
     4801 ttcctggatc gctttttcag tgtcctcgta gccgccttcc ccgatatgat cgtagacgat
     4861 atttccgtcg acatcgatga ggtatttcct cggccagtac tggttgccga aggcattcca
     4921 cgttccgtat tcgttgtcga gcacgaacgg atacttgatt ccgagccgct ccaccgcgtc
     4981 ctccacattc ttttggactt tctcaaacga gaactcgggc gtatggatgc cgatgatttc
     5041 gagcccctgg tcggaatatt tgtcgtacca cgccttgatg tacggcaacg tgcgctggca
     5101 gttgatgcag ctgtacgtcc agatgtcgac gagtaccact ttcttcccct tgaactcgcc
     5161 gaaggttatc ggcttgccgc cggtattgat gtacccgtcg gggctcgtga tctcgggcgc
     5221 ggggccttcg tcgtggagaa gcgagccgcc cggacgcgcc gccgtaatat tcccttgcgg
     5281 cgtcttgaaa tacagcaccg ctccggcgat gatcgcgagc gcaatgactg ctgaaattat
     5341 cacttccggc ttcttgagat ttttcatcac atttgatcgt tgctcaaaaa tctctgttcg
     5401 attttcgtcg catcgaaata attattattt accaggttcg tctccgccgc gcggtcaacg
     5461 cccgtcccga tggcgatgcc gacgaggatg agaagcacgc cgaccgtttt ctggagccag
     5521 cccttcggat cggcggccgc cccgagcttc atgagaagct tctgcccggc gagcgagacg
     5581 atgagaagcg tgaggcagag gccgagcgaa tacgaaagaa gatagacgat gcccaaggcg
     5641 cggttctgcg gcaagaccgt cgcgagcacc acgaagtacg tcgggctgca gctcgagaag
     5701 acgggcccga gcgccgcgcc cgtgagaagg tcgccccaga agtcgtggcg ctgataccct
     5761 tctgccatga ttgcgttcga ttttcggccg agagcgccca caagggcgat cctcgcccac
     5821 cagtccggaa agagcaaaga gaggccgaga agcgcgataa tgccgcctga gatccattcc
     5881 cagaccgact gcggcacgct gatgagaagc gttgacacct tgagaagcag ggtgaagagg
     5941 aagaccgacg cgccaaggct gagcgtcacc acatacgcct tcttcctatt agcccctccc
     6001 gcgaccgagc cgccgacaat gacgggaatc atcggcaaaa cgcacggcgt gatgacggtg
     6061 aggattcccg caaggaatgt gatgagaagc agaagcatta tttaaattat acgccaagtt
     6121 tttagcaaaa tttcaatcta taatatttgc ggctgccgct cgcctcacgg atattcgcct
     6181 gctggcgaat tccgcgagcc gccttgcgcc atcgcgctcc ggccttgcga aagctgcgcc
     6241 tgctcgcttt ctcacgcaga aatgcgccga atttgggaat tttgttaggg agcgaagcgg
     6301 aacgtatcct cccacggctc attcccctcc ccgcttggaa tggcatattg gccgatgtcg
     6361 aaaaaggcga aaattccagg atgatttgta tcgtagagaa agtagcgcag gccgttcgtt
     6421 ttcgcatacg cctcattgca gagcgaagca ttcgcccatg cgggcaacga agggcttgat
     6481 gctgccgtcg gcggcacgat ctcggcggtc atgtagccgc cttcatattt attcgtctgc
     6541 tcgatatcga tcgtgcagtc gttggaattt ccggcgagcg cgatcctgcg gatcgctgcc
     6601 tcaaacgtct ccttcggatc cttcttgaac accgctaccg attggccgga cgccggctcg
     6661 ctgcctttct catagacata cactttcgta cccgtcgctt ccgtttcgac gcccggcccg
     6721 tattggaacg cgatgccgag gtcgcgatcg aggaagatgc ctgaggcatt cgcgctctgc
     6781 tccgcaacct ggccgaagtc ccggggcgca ttagcctgca atcgggcctg aatgagtcca
     6841 tacgcggaga ctgcaatcag aaggaccgcg actatgaccg cggcaatcaa aagaatgcgg
     6901 aatgctttca tgcatcaaag tataccagaa gcgag
//
LOCUS       DASZYS010000025         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_221086, whole genome shotgun sequence.
ACCESSION   DASZYS010000025 DASZYS010000000
VERSION     DASZYS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221086"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VHF05_01520"
     CDS             complement(<1..301)
                     /locus_tag="VHF05_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79635.1"
                     /translation="MEFLKLVLLAFLGMAFAVGGVTSFSRPKQQRRRWYQKILRRIWE
                     SACMVGGFVLAISNGANLVYALEESGPFIHYGIPILLGILALGLLKKFLDIYHTYY"
     gene            complement(402..1664)
                     /locus_tag="VHF05_01525"
     CDS             complement(402..1664)
                     /locus_tag="VHF05_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HVS79636.1"
                     /translation="MKNKQALQENGGAARAAGALAVKGFAHMIGEADRTADTSMYYGF
                     TPVKAPELKQEDLALAKQLASREALRRPEIADKEELEPVSNAEEKIAIIRSYMENGMQ
                     ELPHPLMLYYRKPFPGSGLRRQPRDAHFGLDIIGSPRGVSEAIIIQTIVACLKEDGFD
                     QFIVELNGVGDKDAMAKHERELGLYYKKHSGTLDQKFKTTFKQSPFYMLTETDAECRS
                     FCEGAPKSVSYLGEAGRRHFMEVLEYLESLDIPYRLNHALLGHQHFASHVLFKIKIVR
                     DDGSETTVATGARYNNLSRKLGFKKELPAMSATVRYRRAAAVSDRFRPIAPPKFYLVQ
                     FGNDARRKCLSLIDMLRRERIAVHHSLTRDKLLPQMAAAESLKVPFVLIMGQKEALDG
                     TVVVRNIGTRAQETLRLDELIPYLRKLK"
     gene            complement(1729..2361)
                     /gene="lepB"
                     /locus_tag="VHF05_01530"
     CDS             complement(1729..2361)
                     /gene="lepB"
                     /locus_tag="VHF05_01530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVS79637.1"
                     /translation="MEDNRTTTSHPVPPVTPPKKDREQGGGFFSELFKFALIALFIVL
                     PFRIFIAQPFIVNGASMDPTFKSGDYLIVDQLSYRFENPPRGSVVVFKYPKDTTKYFI
                     KRIIGLPGETVSIQGSSVTIKNAENPAGFTLDEPYIQFPKTDDNLTVTLGPTQYFVMG
                     DNRLGSSDSRAWGPVDKSLIIGRPILRLFPLGEIGIFPGGYDETGAAAQS"
     gene            2544..3134
                     /gene="pth"
                     /locus_tag="VHF05_01535"
     CDS             2544..3134
                     /gene="pth"
                     /locus_tag="VHF05_01535"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HVS79638.1"
                     /translation="MKYFLVGLGNPGREYENTRHNTGRIVLSHVMKKFDFDDLGENKK
                     LKALSAKGEIGGKDVLAIEPETYMNKSGSSVKEAVKNPKDAARMIVVYDDMDLPLGSF
                     RIAWNRSSGGHNGLESIIKSLRTREFARIRVGVSPATPKGKAKKPSGEEKVIKFLMGK
                     FKPDEMDEIKKASKKIAEAIEVMIAESREKAMTLFN"
     gene            complement(3387..>3728)
                     /locus_tag="VHF05_01540"
     CDS             complement(3387..>3728)
                     /locus_tag="VHF05_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79639.1"
                     /translation="EIWISRIPENLLDMKTIEKLCPNGKAENMHYLSPETDISGSLLA
                     ARCLNPYSSCDGKYPPLDEADLISPTEAPRMRLFQFASRNASDASALETAPTATRDVE
                     LHEPPLLSAPK"
BASE COUNT          825 a          942 c         1109 g          852 t
ORIGIN      
        1 agtaatacgt atggtagatg tccaggaact ttttcaataa accgagggct aaaattccga
       61 gcagaatcgg aattccgtaa tgtatgaacg gacccgattc ttcaagggcg tacactaggt
      121 tggcgccgtt ggagatagcg agcacaaaac caccaaccat gcacgcgctt tcccatatcc
      181 ttcgcagtat cttttgatac caccgccgcc gctgctgttt gggtcgagag aagcttgtga
      241 ctcctccgac tgcgaacgcc atgcccaaaa aagctaaaag tacgagtttc agaaattcca
      301 ttgccgcctc ctgatttaat tatacaccat tttttattaa taagtcaact ttagtaaaaa
      361 acgccccggc tggggcgttt ttccgaaagc tgagattatg gttatttcag cttcctaaga
      421 tagggaatga gttcgtcgag gcggagggtt tcctgggcgc gggtgccgat attgcggacg
      481 accaccgtac cgtcgagggc ttccttctgg cccatgatca atacgaacgg caccttgagg
      541 ctttcggccg ccgccatctg aggcaggagc ttatcgcggg tgagggaatg atgcaccgcg
      601 atccgctcgc gccggagcat gtcgatgagg gagaggcact tgcggcgggc gtcgtttccg
      661 aactggacga gatagaattt cggcggcgcg atcggcctga acctgtcgga aaccgccgcg
      721 gcgcggcggt aacgcaccgt cgcgctcatt gccggaagct cctttttgaa gccgagcttt
      781 cttgagaggt tgttgtagcg ggctcccgtg gcgactgtcg tctcgctgcc gtcatcgcgt
      841 acgatcttta ttttaaagag gacatgcgac gcgaagtgct ggtggccgag gagagcgtgg
      901 ttgaggcggt aggggatgtc gagcgactcg aggtactcga ggacttccat gaaatgccgc
      961 cggcccgctt cgccgaggta gcttaccgac ttcggggcgc cttcgcagaa cgaccggcat
     1021 tcggcatccg tttcggtgag catgtagaac gggctttgct tgaaggtcgt cttaaacttc
     1081 tggtcgagcg ttccggagtg cttcttgtag tagaggccga gctcgcgttc gtgcttcgcc
     1141 atcgcgtcct tgtcgccgac gccgttcagc tcgacgatga actggtcgaa gccgtcttcc
     1201 ttgaggcatg cgacgatcgt ctggatgatg atcgcttcgc tcacgccgcg ggggctgccg
     1261 atgatatcga ggccgaagtg cgcgtcgcgc ggctggcggc ggaggccgga gccggggaac
     1321 ggcttgcgat agtagagcat caagggatgg ggaagctcct gcatgccgtt ctccatgtag
     1381 gagcggatga tagcgatctt ctcttccgcg ttggaaacgg gctcaagctc ttctttgtcg
     1441 gcaatctcag gcctgcgcag ggcttcgcgg ctcgcgagct gcttggcgag cgccagatct
     1501 tcctgtttaa gttccggagc tttcaccggc gtgaagccgt agtacatcga cgtgtccgca
     1561 gtgcgatcag cttcgccgat catgtgggca aatcccttga ccgcgagcgc tcccgccgcc
     1621 cgcgccgctc cgccgttttc ctgaagagcc tgtttgttct tcacgcttgt gcgaagttaa
     1681 cttgtaatca tccttccgac gtatggaaaa acttctctgt tccgtctcct atgactgcgc
     1741 tgccgcaccg gtctcatcgt atccgcccgg gaaaataccg atctcgccca atgggaagag
     1801 ccgcagaatc ggcctgccga tgatgaggct cttgtctacc ggtccccacg cgcgggaatc
     1861 cgaactgccg aggcggttgt cgcccatgac gaaatattgc gtcggtccaa gcgtcacggt
     1921 gagattgtcg tcggtcttcg ggaactggat gtacggctcg tcgagggtga atcctgcggg
     1981 attttccgcg ttcttgatag tcactgaaga gccctggatg gagactgtct cgcccgggag
     2041 gccgatgatg cgcttgatga agtacttggt cgtgtccttc gggtacttga agacgacgac
     2101 cgagccgcgt ggcggattct caaagcgata ggagagctgg tcgacgatga gatagtcgcc
     2161 gctcttgaag gtgggatcca tcgacgctcc gtttacgatg aaaggctggg cgatgaagat
     2221 gcggaagggg aggacgatga aaagggcgat cagcgcaaac ttgaagagtt cggagaaaaa
     2281 tccgccgcct tgttcgcgat cctttttcgg aggagtgacg ggtggaaccg gatgcgaggt
     2341 cgttgtgcga ttatcttcca tttgatagtg aatgattaca aagattttat tatggaaagg
     2401 atatttgacg caagagagtt gaacgtgttg cgagcgaaaa atgaaaataa aaatgcaaaa
     2461 aagaaattta aaaaatgata acgatatttt ttggcttcgg acagcagttt tgacttttga
     2521 cttttttaag tacacttaaa tccatgaaat acttccttgt tgggctcggc aatccgggac
     2581 gcgaatacga gaatacgcgg cacaataccg gccgcatcgt gctctcgcac gtgatgaaga
     2641 agttcgattt cgacgatctg ggcgagaata agaagctcaa ggcgctctct gccaagggcg
     2701 agatcggcgg caaggatgtt ctcgcgattg agcccgaaac ctatatgaac aagagcggat
     2761 cgtctgttaa ggaggcagtg aagaatccga aggatgcggc gcgcatgatc gtcgtctatg
     2821 acgacatgga cttgccgctg ggctccttcc gcatcgcctg gaatcgcagt tccggcggcc
     2881 acaacggcct ggagtcgatc atcaaatcgc tccgtacccg cgagttcgcg cgcatccgcg
     2941 tcggcgtatc gcccgcgact cccaagggca aggcgaagaa gccttcaggc gaggaaaagg
     3001 tgatcaaatt tctcatgggc aagttcaagc ccgacgaaat ggacgagatc aagaaagcct
     3061 ccaaaaaaat cgccgaagcg atcgaagtga tgattgccga aagcagggaa aaggcgatga
     3121 cgcttttcaa ctgaccaaaa ataagaagca cttctgagtg cgactatatt tttggtcatc
     3181 ccgcagcagt gagaaagcga atgcctggaa ctttcgcaag gccggaggtt gaccgagccg
     3241 aggcggccgc tgcaggcttt ctcagcagag aaagacctgt ggcgggatcc agtatcttcg
     3301 tttttccgtc atcccatagt agcgggacga cggaggacaa aaaacagccg ccgaagcctc
     3361 tttgagaggt tcggcggctt cttgaattac ttcggtgcgg agagcaatgg cggttcgtgc
     3421 aattcgacat cgcgagttgc ggtaggcgcc gtttccagtg ctgatgcgtc gctcgcgttt
     3481 cgcgatgcga actgaaagag ccgcatgcgc ggagcttcag tcggagatat gagatccgct
     3541 tcgtcgagcg gcggatattt tccatcgcag ctcgaatagg ggttgaggca tcgtgcagcg
     3601 agcaaagagc cgcttatatc cgtttccggg gaaagatagt gcatgttctc ggcttttcca
     3661 ttcgggcaga gcttctcgat cgttttcata tcgagcaaat tttccggaat gcgcgatatc
     3721 catatctc
//
LOCUS       DASZYS010000026        10654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_224721, whole genome shotgun sequence.
ACCESSION   DASZYS010000026 DASZYS010000000
VERSION     DASZYS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224721"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VHF05_01545"
     CDS             <1..378
                     /locus_tag="VHF05_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79640.1"
                     /translation="PLPKRSGFCYMGLLTKALTRQSDRRIKIQEQFHCILKEYREHMT
                     GSAPITTPNIEEAAEKVKKIAHDSDYNPKKVTEEDKNFLRTMAQYSARIMRLIRKQFD
                     FRATWRKLMGLLQPYGWPVTCFV"
     gene            complement(453..2243)
                     /gene="typA"
                     /locus_tag="VHF05_01550"
     CDS             complement(453..2243)
                     /gene="typA"
                     /locus_tag="VHF05_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HVS79641.1"
                     /translation="MEIRNIGIIAHVDHGKTTLTDALMRQTGMVEEGVTMDSNALEQE
                     RGITIYAKNASLFYKGTKINIVDTPGHSDFGSEVERVLRSIDTVLLIVDAQEGPMPQT
                     RFVLKKSLELGLKPIVILNKIDKPAARPAEVHDEVLELFMELGASNEQLDFTCVYAIG
                     RDGIAKKNLTDDSKDLTPLLDTILAEVPPAPADTTKPLRLQPFNLAYDNFLGRLAICR
                     VYEGKIKVGDTLTVFKEDTKSTGKVTKLFTFEGLKRVESQEVEAGDIVMVAGFPDIYI
                     GDTLAGSPDVEPLPRIRVDEPTISMNFLVNDSPFAGRDGKFVTSRQIRERLEKELEVN
                     VGLKIDFSSADAFAVYGRGELHISILLENMRREGFELSVSQPKVIIKEEGGVKLEPYE
                     EATIDVKDDLASTVISKLAERKGILMNMKSANGITRLTFEIPTRGLFGYRGQFVIDTR
                     GLGLLATRFIEFRPYAGEIRKQAFGSMISMIGGKALAYALDNLQQRGTLYIEPGDEIY
                     EGQVIGNTSKGDELYVNPVKGKQLTNMRASGSDGTIILAPAYKLNIERAMEIMADDEF
                     IEVTPNAVRLRKQHLTQIDRTRAMRKEGAA"
     gene            2525..3808
                     /gene="eno"
                     /locus_tag="VHF05_01555"
     CDS             2525..3808
                     /gene="eno"
                     /locus_tag="VHF05_01555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HVS79642.1"
                     /translation="MAKIKDVKAREILDSRGNPTVEVDVMFADGSFGRAAVPSGASTG
                     THEAVELRDGDKKRYGGKGVRKAVANVDGAIKKMVKGKDFDQRSLDAALIKLDSTPNK
                     GKLGANAILGVSMAFAHAAAASQKKPLYKYFKDISKAKAMRLPTPLMNILNGGAHAAG
                     STDLQEFMIVPVGAKSWSDALRYGAEVFQVLKGLLKKKGFSTTVGDEGGYAPALGGNE
                     KALELIVEAIKAAGYKPGKDIAIAIDAASSEFYKNGKYELEIEGRTLSSEEMVDLYAD
                     WIKKYPIVSIEDGLAEDDWQGWQLLTKKIGKKVQLVGDDLYVTNIERLQRGIDEKAGN
                     SILIKLNQIGTVSETIDAINLAHKNGMTAVVSHRSGETEDVTISHLVVGLGTGQIKTG
                     SLCRSERIAKYNELLRIEEELGKKAVYAGKDAFKK"
     gene            4019..4675
                     /locus_tag="VHF05_01560"
     CDS             4019..4675
                     /locus_tag="VHF05_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HVS79643.1"
                     /translation="MKKKTAFILVGLPGCGKGTNGKRYVAEASDHPIHYIETGDLLRR
                     RCTIDREFAEKWQPAMNAGKILPNHVLNELAYQKIDELLADRDFIFDGYPRTLGQAQY
                     IVSLMREVHRYELVTVFFELRDKSAVIDRIRKRSLAESDPLRPDKPENEKHNQQVIRR
                     ADDKIEIVVNRIREFDNEFPTLDPYLRENTTYVVVSADNPPDIVFSHFSQHIMAYAGK
                     "
     gene            4888..5562
                     /locus_tag="VHF05_01565"
     CDS             4888..5562
                     /locus_tag="VHF05_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVS79644.1"
                     /translation="MRILIVEDEERLAKSLKIGLEKEGYAADYVTDGEAAERRITLCH
                     KDYDLVIMDWMLPKKDGVEVCKSVRAQGIKLPILMLTARFDTTDKVNALDMGADDYLV
                     KPFSLEELIARIRALLRRPQVALPTVLKIQDIELDPSTRRVTVNNREIKLTVKEFALL
                     EYMMRNPNQVINRNQILDSLWGFDFDSFSNVVDVHMKNLRKKLNNRRNKVLETVRGIG
                     YKMRME"
     gene            complement(6393..7265)
                     /locus_tag="VHF05_01570"
     CDS             complement(6393..7265)
                     /locus_tag="VHF05_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79645.1"
                     /translation="MMKFGVIKINKIIAAEAACIIIVVLALCLLIRSEIERHPAATPP
                     MATEQPSPYPSTLEESDGFDTSASPLYWLNSGAEALLSSSSSWSTVKGDLAAGSKWHE
                     EYASSNSTDTDGGIHPQNLFRIFTRGVYGDGAYSAYAKIDRIVASASPNRNSSNGVFL
                     MIHAEDSDNLYYAGLRVDGNAVIKKKIGGTYYTIAETPLFAYAPYNRDTRPDLLPLNT
                     WLGMKADVKTLPDNSVQIEFSIDLSGEGSWIPILSVTDAPKGNVQTLPGPGHAGIRSD
                     FMEMSIRDFRVDSR"
     gene            complement(7268..8914)
                     /locus_tag="VHF05_01575"
     CDS             complement(7268..8914)
                     /locus_tag="VHF05_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79646.1"
                     /translation="MLDKRINRIKIYFFAISGIVVIALFAAVVGLKDNPYLTKSYLES
                     YITNWKYQFEFSRMKGAALASAALGDGTGTEATEADVENSGSDIPVLVYHGVLKKSDG
                     SGINLTDEQFKNQMFALKQAGYTTITPDQFLRYLRGEIVLPRKSFLLTFDDGRKDSYY
                     NGDPILKTVGYNATMFVISHFPIDLDGTNYYLSKEELQQMQDSGRWDLEAHTYNGHGM
                     IQIDQGGDTGYFFGNKAWLPSEGRLETDQEYAERISEDLAQVKLQLENTYHKQVNSFA
                     FPFGDHGQEATNYPDADQVLIDHASALYPLLYFQYGGGYRFNANYFNIADAAKQSFLV
                     RRIDINPNWSGADIVKVMNNAEPKILPYTDAFATNNGWIASWGLTKIANGSLTITPEE
                     ANAGAATVLDGTGGWKDYQAHFVVNAQNQNGVYIWVRFQDDNNNAACNFGNGFAHVEE
                     TVNGKENVLQGATNDSIRIPAGDFGVTVKVEGRRLTCTLDNGVSVQSDFLDPSLNQGG
                     IGFKIWEHPNGGSDLTVKRIDATSISDVPVAASKADDPSA"
     gene            complement(9014..10351)
                     /locus_tag="VHF05_01580"
     CDS             complement(9014..10351)
                     /locus_tag="VHF05_01580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVS79647.1"
                     /translation="MNTRALDITKSYEEKRRLDWAYVTLLMVIAGIALFFIALVKSKT
                     ASELLSNPFLLAYSIFVTAFQLSRLVSAIFFKHSYAQVLSSISPAVLEAYEPTVSFVI
                     PCKNEEGAIRNTVTKCFDAAYPEDKKEVIVINDGSTDRTGEILEELKSEYPRLTVITF
                     EKNQGKRYGMAAGFKAATGEIVIQLDSDSYFEPKSFREVLKPFANPEIGGVCAHADPE
                     NADQNVFTKMQAAYYFMSFRILKAAESTYGVVFCLSGCSSAYRRSVVMPHLDAWLKER
                     FLGLPVTWGDDRGLTNVVLREGYKAIYTDVAKAYTIVPSNFKQLVKQQVRWKKGWFVN
                     SIFASKFIMKRDPFVAFFYFFPLTLVTLMTPFMATRAVIYNPLYHHNATILFFITGVF
                     LVAALIVVFYRFVGRDNKYWPYIFAWTALNSVFLAFLLFYALATIQNRKWGTR"
     gene            complement(10373..>10654)
                     /locus_tag="VHF05_01585"
     CDS             complement(10373..>10654)
                     /locus_tag="VHF05_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVS79648.1"
                     /translation="ESPSFEIIRHLEELGANVKVFDPYVKDKSTVSSLEEALEETDAV
                     VIATDHREFTRLSPQEFISNNVGVIVDGRNCLNEEPFVSSGIVYKSIGR"
BASE COUNT         2410 a         2750 c         3030 g         2464 t
ORIGIN      
        1 ccgcttccta aacggagcgg tttttgttat atggggttgt tgacaaaagc attgactcgt
       61 cagtcggata ggcgtataaa aatacaagag cagtttcact gcattttaaa ggagtacaga
      121 gagcatatga caggtagtgc acccatcact acgcccaaca ttgaagaggc agcagaaaag
      181 gtcaagaaaa tcgcccacga tagcgactac aaccctaaaa aggtcactga ggaggacaag
      241 aatttcctcc gcaccatggc ccaatattcg gccaggataa tgcgacttat tcggaaacag
      301 tttgactttc gggcgacctg gcgcaaactc atggggttgt tgcaacccta cggatggcca
      361 gttacctgct ttgtctagca gaaaaaggtc cggacatgca tgtgttccgg accaattttg
      421 aaatctcttt tttacctctc ttcggccggc ttttatgccg cgccctcttt tctcatcgct
      481 cgcgtgcggt cgatctgggt gagatgctgc tttcggaggc gcaccgcgtt cggcgtgact
      541 tcgatgaatt cgtcgtccgc catgatctcc atggcgcgtt cgatattgag cttgtaggcg
      601 ggagcgagga tgatcgtgcc gtccgagccg gacgcgcgca tgttggtgag ctgctttccc
      661 ttgaccggat tcacgtagag ctcgtcgccc ttcgaggtgt tgccgatgac ctggccctcg
      721 tagatctcgt cgcccggctc gatgtaaagc gtgccgcgct gctggaggtt gtcgagagca
      781 taggcgagcg ccttgccgcc gatcatcgag atcatcgagc cgaaggcctg cttgcggatt
      841 tctccggcgt acggcctgaa ctcgatgaag cgcgtggcga gcaagcctaa accgcgcgtg
      901 tcgatcacga actgcccgcg gtagccgaag aggccgcgcg tcgggatttc gaaggtgagg
      961 cgggtgatcc cgttcgccga tttcatattc ataaggatgc ccttgcgctc ggcgagcttc
     1021 gagatgacgg tcgaagcgag atcgtccttc acatcgatag tcgcctcctc atacggctcg
     1081 agcttcacgc cgccctcttc cttgataatg accttgggct gggacacgga aagctcgaag
     1141 ccctcgcggc gcatgttttc gaggaggatc gagatatgga gctcgccgcg gccgtagacc
     1201 gcgaacgcgt cggccgatga gaagtcgatt ttgaggccca cgtttacctc gagctccttt
     1261 tcgaggcgct cgcggatctg gcgcgaggtg acgaacttgc catcgcgccc ggcgaacggc
     1321 gagtcgttga cgaggaaatt catcgagatg gtcggctcat cgacgcgaat gcgaggaagc
     1381 ggctcgacat cgggagagcc tgcgagcgta tcgccgatgt agatgtcggg aaagccagcc
     1441 accatgacga tgtcgccggc ttccacttcc tgcgattcga cgcgcttcag gccttcgaag
     1501 gtgaagagct tcgtcacttt gccggtcgat ttcgtatctt ccttgaagac ggtgagtgtg
     1561 tcgccgactt tgattttacc ttcgtagacg cggcagatgg cgagacgtcc gaggaaattg
     1621 tcgtacgcga gattgaacgg ctgaaggcgc aatggcttcg tcgtgtcggc gggagccgga
     1681 ggcacttcgg cgagaatggt gtcgaggagg ggagtgagat ccttcgagtc gtccgtgaga
     1741 ttcttcttgg caatgccgtc gcggccgatg gcataaacac aggtgaaatc gagctgttcg
     1801 ttcgaggcgc cgagctccat gaaaagctcg agcacttcgt catggacttc cgcggggcgc
     1861 gcggcgggct tgtcgatctt gttcaaaatg acgataggct tcaatcccag ttcgagcgac
     1921 ttcttcaaga cgaagcgggt ctggggcatc gggccttcct gcgcgtcgac tatgaggagg
     1981 acagtgtcga tggagcggag cacgcgctcg acttcggagc cgaagtcgga gtggcccggc
     2041 gtgtcgacga tgttgatctt cgtgccctta taaaagaggg aagcgttctt ggcgtaaatg
     2101 gtaatgccgc gctcctgctc gagggcgttc gagtccatgg taacgccttc ctcgaccatt
     2161 ccggtctggc gcatcaaagc atcggtaaga gttgtcttac catgatcaac atgcgcgatt
     2221 attcctatat tccttatctc catatttttg cggactgcaa atttggcgtt ctcctgcgtt
     2281 gctcgtgaaa tttgcttctg cgcagtacta gtttgtttgt acagcttagt cgccaatttc
     2341 gttcgcgcct tggattacat ccacatctgc gagtccttcc taactatcgc tcaataaaaa
     2401 acaccttccg gtgcaaaaca gcgaagcctt atttaaacaa ttttcagtct ttttgtccag
     2461 ttatttcaat attaaagcgg cggatgatat acttttcgcg ttaaaaactt aaataatcgt
     2521 agagatggca aagataaagg acgtaaaggc acgtgaaatt ttggattcgc gcggcaaccc
     2581 gacggtcgaa gtggacgtaa tgtttgccga tggcagtttc ggacgcgcgg cggttccttc
     2641 gggagcatcg actggcacgc acgaggcggt ggagcttcgc gacggcgaca aaaagcgcta
     2701 cggcggcaaa ggcgtgcgga aagcggttgc caacgtggac ggcgcgatca agaagatggt
     2761 caagggaaag gatttcgacc agcgctcgct cgacgccgcg ctcataaagc tcgatagcac
     2821 gccgaacaag ggcaagctcg gggcgaacgc gattctcggc gtttcgatgg cctttgcgca
     2881 tgcggccgcg gcaagccaga agaagcctct ctacaaatac ttcaaagaca tttcgaaagc
     2941 caaggccatg aggctcccga ctcctctcat gaacatcttg aacggcggcg cgcacgcggc
     3001 cggttcgacg gatctccagg agttcatgat tgtgccggtg ggggccaaaa gctggagcga
     3061 cgcgctccgc tacggcgcgg aggttttcca ggttcttaaa ggcctcttga agaaaaaggg
     3121 cttctcgacg accgtgggcg acgagggcgg atacgctcct gctctcggcg gcaacgaaaa
     3181 ggcgctcgaa cttatcgtgg aggcgatcaa agccgcgggc tacaagccgg gcaaggatat
     3241 cgccatcgcg atcgacgccg cgtcgagcga attctataag aacggaaagt acgagctcga
     3301 gatcgaaggc aggacccttt cgagcgaaga aatggtcgac ctctatgccg attggattaa
     3361 gaaatacccg atcgtctcga ttgaggacgg cttggccgag gacgattggc agggctggca
     3421 gcttctcacc aaaaaaatcg gcaagaaagt ccagctcgtg ggcgacgacc tctacgtgac
     3481 gaacatcgag cggctccagc gcggcatcga cgagaaggcc ggcaactcga tcctcatcaa
     3541 gctcaaccag atcggcacgg tttccgagac gatcgacgcc ataaacctcg cgcataagaa
     3601 cggaatgacc gccgtcgtgt cgcaccgctc tggcgagacg gaggacgtta ccatttcgca
     3661 tctcgtcgtc ggactcggca ccggtcagat caagacaggc tccttgtgcc gcagtgagcg
     3721 catcgcgaag tataacgagc ttctccgcat cgaggaggag cttggtaaaa aggcggttta
     3781 cgccggaaag gacgcattta agaaataagg atttccggat tttaaaccag aattgagaaa
     3841 tgaatgtaat aaaacctttc gatatatcga aaggttttat tactatcgga taagttacta
     3901 cgatatatcg tagtaccatg ctggaaaagg ggacagagag cttgttaaaa atgccttgcc
     3961 aggcggcttt ttcaggtgta ttctttaccc ggattttttc tctgaaaggg gtatttgttt
     4021 gaaaaagaaa acagccttca ttcttgtcgg attgccggga tgcggcaagg gcacgaacgg
     4081 aaaacgctat gtcgctgaag cttcggatca tccgatacat tacattgaga ccggagattt
     4141 gctccgcaga cgctgtacga tcgataggga attcgccgaa aaatggcagc ctgccatgaa
     4201 tgcaggtaag attctgccga atcatgtact caatgagctt gcgtatcaaa agatcgatga
     4261 acttttggcc gatcgcgact tcatatttga cgggtacccg cgcacgctgg gccaggcgca
     4321 gtatattgtc tcgctcatgc gcgaagtgca tcgatacgaa ctcgtaaccg tattcttcga
     4381 attaagagac aaaagcgcag tcatcgacag gatcagaaag cgttctctgg ccgaaagcga
     4441 cccgcttagg ccggacaaac cagaaaatga aaaacataat cagcaagtaa tcagacgcgc
     4501 tgacgataaa atcgaaatcg tggtaaatcg catccgtgaa ttcgataatg aatttccgac
     4561 actcgatcct taccttcgcg agaacacgac atatgtcgta gtctccgcag acaacccgcc
     4621 agacatcgtg ttttcgcact tttcccagca catcatggcc tacgcaggga aatagcctcc
     4681 tcgttcttta tatttatggc gcacagctct actgtgcgcc attttttatc gaaagtaagg
     4741 ccgcgagccc ttcatgaacc ttgaaaattg ccaaaaaatg ttttagaata aggccaaaat
     4801 ggcatttcat gaagacgatt cactaaaact tcatgctcca aaagatagga ttaaggggca
     4861 gttttagcac attaaatctt gccacctatg agaattttaa tcgttgaaga cgaagagagg
     4921 ctcgcgaagt cgctgaagat cggccttgaa aaggaagggt atgccgccga ctacgtcacg
     4981 gacggggagg ctgcggagcg ccgcattacc ctctgccaca aggactatga tctggtcatt
     5041 atggactgga tgcttcccaa gaaggacggc gtcgaggtgt gcaagagcgt gcgcgcccag
     5101 ggcattaaat tgcctatcct catgctaacc gcccgcttcg atacgaccga caaggtgaac
     5161 gctcttgata tgggggcgga cgactatctc gtgaagccgt tctcgctcga ggagctcatc
     5221 gcgcgcattc gcgcgcttct ccgtcgccct caggtcgccc ttccaaccgt tctcaaaatc
     5281 caggatatcg agctcgatcc gtcgacccgc cgggtgacgg tcaacaacag ggagataaag
     5341 ctcacggtga aagagttcgc gcttctcgag tacatgatga ggaatccgaa ccaagtcatc
     5401 aaccgcaatc agattctcga cagcctctgg ggcttcgact tcgactcatt ctcgaacgtc
     5461 gtcgacgtcc acatgaagaa cctccgcaag aagctcaaca acaggcgcaa caaggtgctt
     5521 gagactgtgc ggggtatagg ctacaagatg cgcatggaat agagggccgc tcggaaatct
     5581 aattttaaaa cacgcataaa gcccgctcgg tcagcgggct tttgctatgt tcgtcccgat
     5641 ttctgctgaa atcgagactg cgcaatgttt taaaattaga attcctcgct ctaacgttca
     5701 agcggaacga atttgcaagg ccaaattcgt gcggaatttt ctattcaaag ctgctctcgc
     5761 acgtatagaa atggatttgc aaaaggttgg aagcatgatg cagtgccaaa gatgtgccat
     5821 cttcatgttc atttcacctg aggtttttat agtaatgcaa cgaaacgagt atgttgttcc
     5881 aaacacaatc acaaatcaaa ctcacgtcga acgatctcga cctcctctcg ggaaagaatc
     5941 tgctttcacg cgctcctccg cgctgaaagc aaatccacgc atcataatgc tataaagtaa
     6001 aaagctctaa attcctaact cctattaaga aagcgcccat ttatctccgg ggcgctttcg
     6061 cttatatatc cagctgtttc agtgtgcttt tggcgtttat ggtatagaat ttttttggag
     6121 gatgtaccat gaaaaagcaa ccacttagga ctgttcccgt ggaacggaag caggttccgt
     6181 gcatattcga tcacaatgcg cttgctgtcg gaaaatattc gctttcgaag ggatgcgtat
     6241 gcaaaagcga aacccgacag gatctttgtt cgcagcatgt aataagcgcc gaacctctag
     6301 gctccatgga actgatctgc atctatgatc ccgagtttta ccaccgattc ggatttcagg
     6361 aaaaagccgc accctagtgc ggctttgctg ttttatcggc tatctaccct gaaatccctg
     6421 atgctcattt ccatgaagtc ggagcggatg cccgcgtggc cgggaccggg aagcgtttgg
     6481 acattgcctt tcggcgcgtc tgtcaccgaa aggatcggta tccagctacc ctcgccgctc
     6541 aagtcgatcg aaaactcgat ctgaacggag ttgtcgggaa gcgtctttac gtctgccttc
     6601 atgccgagcc atgtattcag cgggagaaga tctggccgcg tgtcgcgatt gtatggcgca
     6661 tacgcgaaaa ggggagtttc cgcgatcgtg taatacgtgc cgccgatttt cttcttgatg
     6721 accgcgttgc cgtcgacgcg caatcccgca taatacagat tgtcactgtc ctccgcgtgg
     6781 atcatgagga agactccgtt tgagctgttc ctattggggc ttgcgcttgc cacgatcctg
     6841 tcgattttcg cataggccga atatgcgccg tcgccgtaca cgccgcgggt gaagatgcgg
     6901 aacaggttct gcggatggat tccgccgtcg gtgtccgtgg aattcgaact tgcatactct
     6961 tcatgccatt tcgatccggc cgcaaggtcg cccttgaccg tgctccagga tgacgaactt
     7021 gagagaaggg cctcagcgcc ggaattgagc caataaaggg ggcttgcgga ggtatcgaag
     7081 ccgtccgatt cctcaagggt gctgggatat ggagatggct gttctgttgc cataggaggc
     7141 gttgccgcgg gatggcgctc gatttcgctc cggatcagga ggcagagggc aaggacgaca
     7201 atgataatgc aggcggcttc cgcggcaatt atcttattga tcttgataac gccgaatttc
     7261 atcattctta tgcggagggg tcatccgctt tcgatgccgc gacagggacg tcggaaatgg
     7321 aggtcgcatc gatgcgcttc acggtgaggt cgctcccgcc gttcggatgc tcccagatct
     7381 tgaagccgat gccgccctgg ttgagcgacg ggtcgaggaa gtcgctttga accgacactc
     7441 cgttatcgag cgtgcaggtg aggcggcgcc cctcgacctt aaccgtaacg ccgaaatctc
     7501 cggccggaat ccttatcgaa tcgttggtgg cgccctggag cacgttttcc ttgccattca
     7561 ccgtttcctc gacgtgggcg aatccgttgc cgaaattgca ggccgcgttg ttattatcgt
     7621 cctggaaacg tacccagata tagacgccgt tctggttttg ggcattcacg acgaaatgcg
     7681 cctggtagtc cttccatcct cccgttccgt caaggaccgt cgcggctccg gcgttcgctt
     7741 cctcgggcgt gatggtgagg cttccgttcg cgatcttggt gaggccccac gaggcgatcc
     7801 agccgttgtt cgtcgcgaac gcgtcggtgt agggaagtat cttcggctcg gcgttgttca
     7861 ttaccttcac gatatcggcg ccgctccaat tcggattgat gtcgatcctg cggacgagga
     7921 acgactgctt ggccgcatct gcgatgttga agtagttcgc attgaagcgg tagcctccgc
     7981 cgtactggaa atagagaagg ggatagagcg cggaggcgtg gtcgatgagc acctggtcgg
     8041 cgtccgggta gttcgtcgct tcctgcccat gatcgccgaa cgggaatgcg aacgagttca
     8101 cctgcttgtg atacgtgttt tcgagctgga gcttcacctg cgccaagtcc tcgctgatcc
     8161 gctccgcgta ttcctggtcg gtctcgagcc tgccttccga cgggagccat gccttgttgc
     8221 cgaagaaata gccggtatcg cccccttgat cgatctggat cattccgtgg ccgttgtagg
     8281 tgtgcgcttc gagatcccag cggccggagt cctgcatctg ttggagctcc tccttggaga
     8341 gataatagtt cgtgccgtcg agatcgattg ggaagtggga gatgacgaac atggtggcgt
     8401 tgtagcccac ggttttgagg atcgggtcgc cgttgtagta gctgtccttc cttccgtcgt
     8461 caaaggtgag gaggaatgat tttctcggca ggacgatctc cccgcgaagg tagcggagaa
     8521 attggtcggg agtaatcgtc gtgtatccgg cctgcttcag cgcgaacatc tggttcttga
     8581 actgctcatc cgtgaggttg atgccagatc cgtcgctttt tttgagcacg ccgtggtaga
     8641 cgagcaccgg aatatcgctg ccggaattct cgacatcagc ctccgtcgcc tcggtgcccg
     8701 tgccgtcgcc gagcgccgcg cttgcgagcg ccgccccttt catgcgcgag aactcgaact
     8761 ggtatttcca gttggtgata tagctctcaa gatagctttt cgtgaggtac ggattgtcct
     8821 tgaggccgac gaccgcggcg aaaagcgcga tgaccacgat gccggatatc gcgaaaaagt
     8881 atatttttat cctgttgatg cgtttgtcga gcatgacggc acgaaaattt tcaatttata
     8941 attttcaatt ttcaaataat ttccaatgat ccaattttca ttgaaaattg gaaattgata
     9001 attgaaaatt gcgttagcgc gttccccatt tcctgttctg gatcgttgcc agcgcgtaga
     9061 agagcaggaa cgccaggaag acggagttga gcgcggtcca ggcgaagatg tacggccagt
     9121 atttattgtc gcggccgacg aagcggtaga agacgacgat aagcgccgcg accaggaaca
     9181 cgccggtaat gaagaagagg atcgtcgcgt tgtgatgata gagcggattg taaatgacgg
     9241 cgcgcgtcgc catgaagggc gtcatcaagg ttaccaacgt gagcgggaaa aaatagaaaa
     9301 aggccacgaa cgggtcgcgc ttcattatga acttcgacgc gaagatggaa ttcacgaacc
     9361 atcccttctt ccagcgcact tgctgtttga cgagctgctt gaagttcgaa ggcacgatgg
     9421 tgtatgcctt ggcgacgtcg gtgtagatcg ccttgtatcc ttcacgaagg acgacgttgg
     9481 tgaggccgcg atcgtctccc caggtgacag ggaggccgag aaacctttcc ttgagccagg
     9541 cgtcgaggtg gggcatgacg acggagcggc gataggcgct cgagcagccg gagaggcaaa
     9601 agacgacgcc gtaggtcgat tccgccgcct tcaggatgcg gaacgacatg aaataatagg
     9661 ccgcctgcat cttggtgaag acgttctggt cggcattctc cggatcggcg tgtgcgcaca
     9721 caccgccgat ctcggggttc gcgaacggct tcaggacttc gcggaagctc ttcggctcga
     9781 agtagctgtc gctgtcgagc tggatgacga tctctccagt cgccgccttg aaccccgcgg
     9841 ccatgccgta gcgcttgccc tggtttttct cgaatgtgat gacggtaagg cgcggatact
     9901 cggacttgag ctcttcgaga atctctcccg tgcggtcggt cgagccgtcg tttatgacga
     9961 tgacctcctt cttgtcttcg gggtacgccg cgtcgaagca cttcgtcaca gtgttcctga
    10021 tcgcgccctc ctcgttcttg cagggaatga cgaaggagac cgtaggctcg tacgcctcga
    10081 gaaccgccgg agaaatcgat gagaggacct gggcgtacga gtgcttgaag aatatcgccg
    10141 acaccagccg cgacagctgg aacgccgtta cgaagatcga ataggcgaga aggaacggat
    10201 tggaaagaag ctccgacgcc gtcttcgatt tcacgagggc gatgaagaag agcgcgatgc
    10261 ccgcgatcac cataagaagc gtgacgtagg cccaatcaag gcggcgcttc tcctcgtagg
    10321 atttggttat gtccagtgct cgtgtgttca tggattggtt tttctaggca ggctagcgcc
    10381 cgatactctt ataaacgatg cccgagctta cgaagggttc ttcgttgagg cagtttcttc
    10441 cgtcgacgat gacgccgacg ttgttcgaga tgaattcctg cggcgagagg cgggtgaatt
    10501 cgcggtgatc ggtcgcgatg acgaccgcgt cggtttcctc gagcgcttcc tcaagcgagg
    10561 agacggtcga cttgtccttg acgtacgggt cgaacacctt cacgttcgcg ccgagttcct
    10621 ccaggtgcct gatgatctcg aacgaggggc tttc
//
LOCUS       DASZYS010000027         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1187858, whole genome shotgun sequence.
ACCESSION   DASZYS010000027 DASZYS010000000
VERSION     DASZYS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187858"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VHF05_01590"
     CDS             complement(<1..253)
                     /locus_tag="VHF05_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HVS79649.1"
                     /translation="MAEALIRNKKANFDYETLKSFEGGLELLGFEVKSVRNHQGSLDG
                     SHVSIRGGEAFLLNAHIPPFQPANAPKDYDPYRTRRILLT"
     gene            459..2432
                     /gene="ftsH"
                     /locus_tag="VHF05_01595"
     CDS             459..2432
                     /gene="ftsH"
                     /locus_tag="VHF05_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVS79650.1"
                     /translation="MDNNEKSNNKGKKDIKVVIKKTKEKLAPNGVPGGRNPMWQNIIT
                     TLLIFVVLVGFYTYISGRKAANDTVTLSELALDVKSGSVAQIDVKDDDLVVTYAGGTE
                     KKSQKEQGVGLTETLKNLGVSDQQMSAVKINIETQGGFAYWAEALLPILIPSAAILLF
                     LWYLTRRMKGAGMQAFTFGQSKARMISPHDKGQRVTFKDVAGAKEAKEELSEIVDFLR
                     NPKKFLDIGARIPKGILLMGAPGTGKTLLARAVAGEAKVAFFSISGSEFVEMFVGVGA
                     SRVRDLFKIAKQVAPAIIFIDEIDAVGRVRGVGAGGGNDEREQTLNQILVEMDGFEPN
                     EKVIVMAATNRPDVLDPALLRPGRFDRRVTIDPPDRTDRFEILKIHARQKPLAEDVNL
                     TVIAERTPGFSGADLYSLMNEGAILAARENRSRISQFDLIRSIEKVMLGPERRSHLLT
                     DMEKKITAYHEAGHALVASVLPYADPVHKISIISRGRAAGYTLKLPLEDRKLQSKKEF
                     LDDIAMSLGGYVAENLIFNDVTTGASNDLQVATALARDMVTKYGMSEKIGPVALEGQG
                     GRALFGNGLEEKEYAGNVGNEIDTEVAKIMHGAHEKAVKILTDYRAVLDAITTRLMEV
                     ETIERDEYEKILIAHNIPLKKKQDIEHKGEVSA"
     gene            3093..3165
                     /locus_tag="VHF05_01600"
     tRNA            3093..3165
                     /locus_tag="VHF05_01600"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3126..3128,aa:Ile,seq:tat)
     gene            3377..4267
                     /locus_tag="VHF05_01605"
     CDS             3377..4267
                     /locus_tag="VHF05_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HVS79651.1"
                     /translation="MVAQERSSSLYYQTLKDQLVTRVPYDPHLLSLLFNEFHANRWKK
                     FCDLPLEIKNRFKYPDHQIVGARKQDPGYVLKMEEGKEKDNKETFHYFPENRQFLEYY
                     SLNKLVDSTPVIRNFFSFAEGVYHYSSGIALNILASMAAERQDLAPILLNPHNRFVLR
                     FLHYLGTGEGDILAKQHYDRGGLTVHIHENQPGFEFLSLNRDSSMEWKPVNLQDGFTF
                     AFGSYQMQELTGCEICRVWHRVRRVPGNDIANRTSIVLFVYLIDAPSYPDTHRSQDDV
                     PGYVVDGFFRKYEEVPAGTG"
BASE COUNT         1106 a         1204 c         1275 g          981 t
ORIGIN      
        1 ttgtgaggag gatgcggcgg gtgcggtacg gatcgtagtc tttcggggcg tttgcaggct
       61 gaaagggagg gatgtgggcg tttaagagaa aggcttcgcc gccgcggatg gaaacgtgcg
      121 aaccgtcgag cgagccctgg tggttccgca ctgatttcac ctcgaatccg agcaattcca
      181 agcctccctc aaaggacttg agggtctcgt aatcgaagtt tgcttttttg ttccggataa
      241 gcgcttccgc catgagggta ctatagcatt gagctcctgg tttatgaatt caccggcgat
      301 ccgttgccag aactgccgag taattttcac gtttttctca gggttctttt gttgtatagt
      361 aggtacgtgc tgaaacgaca atatttccac caaggatgca aaaagcattg agagacaagc
      421 ctcgccggta tttagacagg gcattttgaa caaaaagaat ggataacaac gaaaaaagca
      481 acaataaggg caagaaggac atcaaggtcg tcatcaagaa gaccaaggag aagctcgcgc
      541 ccaacggcgt ccccggcggc aggaacccga tgtggcagaa cattatcacc acgctcctca
      601 tcttcgtcgt gcttgtcggc ttctatacat atattagcgg cagaaaggcc gccaacgata
      661 ccgtgacgct ctccgagctc gcgctcgacg tgaagtcggg atcggtcgcg cagatcgacg
      721 tgaaggacga cgatctcgtc gtaacgtacg cgggcggcac cgagaagaag tcgcagaagg
      781 agcagggagt cgggctcacc gaaacgctca agaacctcgg cgtgagcgac cagcagatgt
      841 cggcggtgaa gatcaatatc gagacgcagg gaggcttcgc ctattgggcc gaggcgcttc
      901 tccccatcct tattccgtcg gcggccattc tcctctttct ctggtacctg acgcggcgga
      961 tgaagggcgc cggcatgcag gcgtttacct tcggccagtc gaaggcgcgc atgatctcgc
     1021 cgcacgacaa gggccagcgc gtcaccttca aggatgtggc gggtgcaaag gaggcaaagg
     1081 aagagctttc cgaaatcgtc gacttcctcc gcaatccaaa gaaattcctc gacatcggcg
     1141 cgagaattcc caagggcatc cttcttatgg gcgctccggg caccggcaag actttgcttg
     1201 cccgcgcggt cgcaggggag gccaaggtgg ctttcttctc gatctcgggc tcggagttcg
     1261 tcgagatgtt cgtaggcgtc ggcgcctcgc gcgtgcgcga tctcttcaag atcgccaagc
     1321 aggtggcgcc cgcgatcatc ttcatcgatg agatcgacgc cgtgggccgc gtgcgcggcg
     1381 tcggcgcggg cggcggcaac gacgagcgcg agcaaacgct caaccagatt ctcgtcgaga
     1441 tggacggctt cgagccgaac gaaaaagtga tcgtcatggc ggcgaccaac cgccccgacg
     1501 tgctcgatcc cgccctgctt cgcccgggcc gcttcgaccg ccgcgttacc atcgacccgc
     1561 ccgaccgcac cgaccgcttt gaaatactca agatccatgc ccgccagaag ccgctcgccg
     1621 aagacgtgaa tctcaccgtg atcgccgaac gcaccccggg cttctccggc gccgatctct
     1681 acagcctcat gaacgaaggc gcgatcctcg ccgcgcgcga aaaccgctcc cgaatctcgc
     1741 agttcgacct catccgctcg atcgagaaag taatgctcgg ccccgagcgc cgctcgcatc
     1801 ttctcaccga catggaaaag aagatcaccg cgtaccacga agcggggcac gcgctcgtcg
     1861 cgtcggtgct tccgtacgcc gatcctgttc acaagatctc gataatttcc cgcggccgcg
     1921 ctgctggcta tacgctgaag ctcccgctcg aagaccgcaa gctccagtcg aagaaggaat
     1981 ttctcgatga tatcgcgatg tccctcggcg gctacgtcgc agaaaatctc atcttcaatg
     2041 acgttaccac gggagcttcg aacgatctcc aggtggcgac ggcgctcgcc cgcgacatgg
     2101 tcaccaaata cggcatgtcg gagaagatcg gcccggtagc gctcgagggc cagggcggca
     2161 gggcgctctt cggcaacggc ctcgaagaga aggagtatgc cggcaacgtg ggcaatgaga
     2221 tcgacaccga agttgcgaag atcatgcacg gcgcgcatga gaaggctgtc aaaatcctca
     2281 cggattaccg cgcggttctc gacgcgatca cgacgcgcct catggaagtc gagacgatcg
     2341 agcgcgacga atacgaaaag atcctcatcg cccataacat tccgctcaag aaaaagcagg
     2401 atatcgagca caagggagaa gtaagcgcgt aggaaagtac gaagacaaat cagacaacag
     2461 acagagcagt aaggggaggg gatcacgcac gtctagaact gtcgggtgtt cttacaacaa
     2521 aaagaccgcg catggcgcgg aatttttgtt gcattaaagg ctttgacgga agataaatga
     2581 tttgctttcc tgtatgcgga gcacctgatg accgaaacgc tcataaacag aaagcaattg
     2641 acattccacc cgaggggcga ccatgaaccc gaatattccg atttttccca tcattacgaa
     2701 ccgaatccga gcaagttcct tcggaggacc cgaacgccgt ttctgcgctg ctgcggccag
     2761 cggatgttgc gagtcaagcg catcgaagtg cgcgtatgct ggagatgcat gtttgaagag
     2821 aggttcgaaa taacggactt cgtatattgc aataaatgca aaaagaagcc cgaatacctg
     2881 ggctgatacg gcccgcggag cgatccgcgg gcttttttct tccgaatgtg ataattttta
     2941 gggtgaattc tggcggaaat gcgtgtgtag ctcagttggt tagagcaccg agcttacaga
     3001 tggcagcgag agacaaataa aaatgggcgt atagctcagt tggtagagca gccgtcttat
     3061 aacgcctagt gagtacgatt gaaaacaagt gtccgcctgt agctcagttg gtagagcagc
     3121 cgtcttatac acggccggtc ccaggttcga ttcctggcgg gcggacactc gtttgcagtt
     3181 cgtacgagcg acggcgttat atcagtgata tacacggccg gtcctaggtt cgaatcctag
     3241 tacgcccaca gatttagccc gtttatattt ggatcgagcg aaccatctgt atcgcagcac
     3301 agcggaaata tacctcggcg gtccctggtt cgagcccagg cacacgcaca gatttacaaa
     3361 aacagagggc tcgggtatgg tagcccagga aaggagcagt tctttgtatt atcaaacgct
     3421 taaagaccaa ctggtaacaa gggtgccgta tgatccgcat ctcctgagtc ttttgttcaa
     3481 tgaatttcat gcgaacaggt ggaagaagtt ttgcgatttg ccgttagaaa ttaaaaatcg
     3541 cttcaaatac cccgaccacc agattgtggg cgccaggaaa caggatccgg ggtatgtgct
     3601 caagatggag gaagggaaag agaaggataa taaggaaaca ttccattatt ttccggagaa
     3661 tcggcaattt ctggaatatt attccttaaa taaacttgtc gattccacgc cggttatccg
     3721 caatttcttc agttttgctg agggtgtcta tcattacagc agcggaatcg cgcttaacat
     3781 tctggcgagt atggcagcag aacggcaaga tcttgcgcca attcttctca atcctcataa
     3841 ccgcttcgtc cttcgcttcc tccattacct cgggacgggg gagggtgata ttcttgcgaa
     3901 gcagcattac gatcgcggcg gcttaaccgt ccatattcat gagaatcagc ccggatttga
     3961 gttcctttca ctgaatcggg actcatcgat ggaatggaag cctgtgaact tgcaggatgg
     4021 cttcactttc gcattcggtt cgtaccaaat gcaagagctc accggctgtg aaatctgccg
     4081 tgtttggcat cgcgtgcgaa gagtgccagg caacgacatt gcaaatcgaa cgtcgattgt
     4141 tctcttcgtt tatttaatcg atgcgccgtc atatcccgac actcatcgtt cccaagacga
     4201 tgttccgggg tatgtggtgg atggtttttt cagaaaatat gaggaagtac cggccggtac
     4261 tggataagca agcggcgtgc acaagcacgc cgcttctttg tttgacacta ttttttaaat
     4321 attgcaaaat taaggcagag aagagaagag gtgtatagtg agactgcttg tccttttcat
     4381 atgttgcgcg tccgcgcccg tcttcgggca gaacgcgatg ttcaaacacg caatcgacga
     4441 cttgaaaggc ggatcgcctt cgaggcaatc gcttttggta atcaaccatg tggcattcgc
     4501 ccagaccgcg ttcgtcatga gccggctcgg agaaggcaat ttcggaacgg gtttcaatcc
     4561 gcttcc
//
LOCUS       DASZYS010000028        30158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_95268, whole genome shotgun sequence.
ACCESSION   DASZYS010000028 DASZYS010000000
VERSION     DASZYS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 30158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30158
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95268"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHF05_01610"
     CDS             <1..199
                     /locus_tag="VHF05_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79652.1"
                     /translation="KSEFREAASPKNGGIPWNAAVFWRSRFAKFRFRALSLPSSLFCL
                     ISRFKFDSHSDTKINRHASAG"
     gene            603..1625
                     /gene="ruvB"
                     /locus_tag="VHF05_01615"
     CDS             603..1625
                     /gene="ruvB"
                     /locus_tag="VHF05_01615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVS79653.1"
                     /translation="MSSNTEEVRQEGPDSRYLDQTLRPNTWSEYVGQEAVKKNLVILL
                     SAAKERSHPPEHILFYGPPGLGKTTLAHLIAKEIGAQMKITSGPAIEKVGDLASILTN
                     LSAGDILFIDEIHRLNKAIEEVLYPALESGTLDIIIGKGPSARTIQLDLPPFTLIAAT
                     TRIGLISSPLRSRFSGGVFRLEFYTIPEISEIVRRSAKILGVPLDEKAALEISKRSRF
                     TPRTANYLLKRSRDFAQVYKKDLSKEVVEQALELLGIDAVGLNSSDRAILSTIIEKFN
                     GGPVGLNTISASLSEDQATIEEYNEPYLIQTGFLDRTPRGRVVTEKGYEHMGQTPPAN
                     LQDKLL"
     gene            1725..2252
                     /locus_tag="VHF05_01620"
     CDS             1725..2252
                     /locus_tag="VHF05_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVS79654.1"
                     /translation="MSGHNKWSKIKRQKEKTDAQKSKIFGKMVRVITVEAKKSNGQMT
                     PSLRAAVDAAKAANVPADNIDRAIKKASEPSENLDSITYEAYGPGGCALIIDALTSNR
                     NKAAQEIKHILSKHGFALAAPGSAQWAFTKSVEEGWVPNSTVPISEADGALLETLMSD
                     LEDNDEVQDVYTNAE"
     gene            2383..2877
                     /locus_tag="VHF05_01625"
     CDS             2383..2877
                     /locus_tag="VHF05_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVS79655.1"
                     /translation="MKILGIDPGYERLGIAVIEKEKNAKDQVLFSECFKTLSSLPHPE
                     RLRLLGQEIERVIEEFRPEALGIETLFFNKNQKTAMHVSEARGSILYAAASKGLAIHE
                     FTPGEIKMALSGYGKSDKDQMIKMVNLLVKVRPGIKYDDEYDAIAAGLTLSATLGIKA
                     LTKP"
     gene            3013..3195
                     /locus_tag="VHF05_01630"
     CDS             3013..3195
                     /locus_tag="VHF05_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79656.1"
                     /translation="MFAEELKYASPILAMEDEEEEEDEFKDDEGDSEDDFSGEDEEDD
                     DMDEEEGTDEGEGESY"
     gene            complement(3417..4805)
                     /gene="tyrS"
                     /locus_tag="VHF05_01635"
     CDS             complement(3417..4805)
                     /gene="tyrS"
                     /locus_tag="VHF05_01635"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVS79657.1"
                     /translation="MDLEEKVKKIDDLFSRGVGEFIDPDGAFREKLVKKARGEYDRDI
                     IIKFGVDPTRPDIHLGHAVVLRKLRAFQDLGCKVIFLIGDFTARIGDPTGKSKVRPEI
                     DQREVERNMMTYLEQVGKITLVGEDRFSWIRNSDWFLGVADIQPKPGNLVRFKTTFIN
                     PSSFIGKAVLYDETRMQKTHLKKAHVVSVSLANILSTLRHITHSRLIERDMFRERIEK
                     NEELFMHEMMYPVLQGIDSHVLNLIYGSCDLEVGGTDQTFNMLLGRDIMKMNGQEPQA
                     VLSFKLLEGTDGKEKMSKSLGNYVAISDEPFDMFGKIMSIPDSSIGNYFELCTYMPLS
                     DIEKMQRKIDSGEMNPRDAKELLAEQIVTMYHGKDAAGKAKEGFSSTFKKGGIPDNIP
                     ETSAEKGASLAEVLVNASIVSSKTEFRRLLDEGAIEDLEKGEEIKDAHQKIEEPLILK
                     VGKRRFIKINIK"
     gene            5014..7566
                     /locus_tag="VHF05_01640"
     CDS             5014..7566
                     /locus_tag="VHF05_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVS79658.1"
                     /translation="MRRKVHSLWTRFKELGWKKIVTVSAGVFLILLGIFIIWVSTLKL
                     PTLDSFGESGRLAESTKIYDRTGQVVLYDVNQNLKRNVVSFDQISQHVKDATIAIEDD
                     KFYEHHGVRPLSFLRSAIADVTSMSFSQGGSTITQQVIKNTVLSGEKTVPRKIEEWIL
                     AIRLEQQMSKNDILDMYLNVIPYGGTMYGVEEASMEFFGKHAADLDIAESAYIAALPQ
                     SPTYYSPYGNHKDALEARKNLVLQKMLENGYITQDQYDQAKAEKVTFLPQQNQGIKAP
                     HFVMYIEDYLVQKYGEDALQQGGYKVITTLDYDLQSKAEEAAKEYAASNEKNFNASNA
                     AFIAIDPKTGQILAMVGSRDYFDTSIDGNFNVTTAHRQPGSTFKPFVYAESWLKGYTP
                     ETVLFDVPTQFSVNCAPDNMTDDNGCYSPVNYDDGFRGPMTMRNALAQSINVPAVKTL
                     YLAGIQDTINLAQSMGVGSLGDPKQYGLTLALGGGEVSLLDMTSAYGVFANQGVREPA
                     VGILEIDDRQGNVLEKFQPHESQVLPAEIANEMNDVLSDAVARSPEYGYNSAVNIPGH
                     NVAVKTGTTNDYRDAWVIGYTPDIVIGAWAGNNDNSPMVKKISGFIVAPMWHVVMQYA
                     LDRLPDDQFTPPTIDYAGLKPVLRGQWQGGESYSIDTVSGKLATANTPPEDVQEMFTG
                     GIHSILYWVNKDDPRGPIPTNPSADGQFTNWEYAVQAWAAANNITSGTTTPPTDFDDV
                     HTEGAGPKISFDNPTNDKLYDPDSKILIRLNYDESANIQRVDYYLNDQFIGSSTNEPF
                     SFSFVPSDYGDTKNTNDLSAVATDMLGNRGTVTMRFKVNKSNAVISPPVIRL"
     gene            complement(7538..7795)
                     /locus_tag="VHF05_01645"
     CDS             complement(7538..7795)
                     /locus_tag="VHF05_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79659.1"
                     /translation="MQDITSFFGKFKSLIANGSAQREAIARIMKESIGAEIDPKKIVI
                     KDGIATVPSNPVMRNELLLKKQLVLEKLKSDPATAGLRGVK"
     gene            complement(7939..9039)
                     /gene="dnaN"
                     /locus_tag="VHF05_01650"
     CDS             complement(7939..9039)
                     /gene="dnaN"
                     /locus_tag="VHF05_01650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVS79660.1"
                     /translation="MKIEVPKKKIQEVVSRTEKVAEKNAALPVLKCIHMEAKNKMLTV
                     KATNLELGLEMHIPISIVEEGAVAVPGSVFNSFIANLPDDSMVTVETDGENLRVSTPN
                     SSTVIKTMPTEEFPIIPTVEDGEVFTVDAADFAKGLKSVWWSAAVSSMKPELSSVYVY
                     TDEEEIVFVATDSFRVSEKRVKAKVSRDFGHILIPVKNIPEILRILDAAKGDIEVNAS
                     KNQLSISYENVYLVSRVIDGTFPDYKQIIPKGFKTEAIILKQDLMNSLKLASVFSDKF
                     NQLNINVSPKKKKFELTTRNSDIGESTNNIENSSSGEDITLNVAYKYFSDSFQSIDSE
                     SLSIQFNGLGRPIVIKGVSDPSFVYVMMPMNK"
     gene            complement(9291..10637)
                     /gene="dnaA"
                     /locus_tag="VHF05_01655"
     CDS             complement(9291..10637)
                     /gene="dnaA"
                     /locus_tag="VHF05_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVS79661.1"
                     /translation="MDNKTLWNSVLAEIEMTVSKPNFNTWFKDTFIVKQEDGTVILSV
                     PNAFVKEWLHNKYHSVILKHLRNLSSGVHSLAFTITKSDPNARKDAFERLSASVPTTE
                     LPLNDYYINKEDNLNPRYTFETFIVGPFNELAFAAAQAVVKKPGIVYNPLFIYGNTGH
                     GKTHLIQAIGNYLKNTANKKVYYATSEKFASDYINAVQTNKVSNFKEKYRKCDILVMD
                     DIQFFSKTEKTQEELFHLFNTLYDNNKQIIFSSDQHPNFIPNLEDRLKSRFSAGMIID
                     IPAPDHESKKAILQAKCRMNSFDLSDEVFDYLASSISGNVRDLEGVLNSIIFQSQLKG
                     KELSLLEIKNFIKNNTKPKKTLSPKEVVKIIADFYNIDEESIYEKTRRKEIIKPRQVT
                     MYILREDFNISYPSIGQKLGDRDHTTVIHSCEKIKKDLLEDSTLVQELSQIRAMIV"
     gene            11040..11174
                     /gene="rpmH"
                     /locus_tag="VHF05_01660"
     CDS             11040..11174
                     /gene="rpmH"
                     /locus_tag="VHF05_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVS79662.1"
                     /translation="MSFTYNPKKKKRAKTHGFRSRTKSAGGRRTLRNRRRKGRAKLAV
                     "
     gene            11190..11528
                     /gene="rnpA"
                     /locus_tag="VHF05_01665"
     CDS             11190..11528
                     /gene="rnpA"
                     /locus_tag="VHF05_01665"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVS79663.1"
                     /translation="MLKAEHRLKRKYFKEIFDRGATFRGRYFILKVAKAEDGLPSRFG
                     FSVSKKVYKSAVDRNRTRRQGYSALKNHIDAVNGGIIGLFSMIRTGKRPKFAEISQDI
                     EALLRQSRLI"
     gene            11592..12344
                     /locus_tag="VHF05_01670"
     CDS             11592..12344
                     /locus_tag="VHF05_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HVS79664.1"
                     /translation="MIWFFQAVFYKPLYNGLILLLNAIPGADIGIAVIVFTIIVKLIL
                     FPLTYKSIKTQIQMKRFEPELAKIREKYTDKQEQALKVMEVYKQAGINPFASFFLVLI
                     QLPILISLYYVFLHSGLPTINPDLIYPFVHANRPIAMELFGLMDLSKSSIVISLLAAV
                     ATFFQMKYSASTMGSTGVRKPNASFTDDLARTMTTQMKYVYPVFIFIIAYRLSGAVAL
                     YWLVNTLFTLAQELYARRRIGRNHPPEAEVAK"
     gene            12354..12803
                     /locus_tag="VHF05_01675"
     CDS             12354..12803
                     /locus_tag="VHF05_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HVS79665.1"
                     /translation="MDTAGIISVIGKTLDLLGIEYEAIEEHPSKNDAVRINILTRQSG
                     RLIGTRGETLSALNLIVKKMVSSANESTHISVDVNGYLEAVDQAIRNKTYILAERAKS
                     FKTDVEMEPMSAYDRMVAHSFLKDDPHLETVSIGAGKNRRIVIKYKE"
     gene            complement(13002..14243)
                     /locus_tag="VHF05_01680"
     CDS             complement(13002..14243)
                     /locus_tag="VHF05_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HVS79666.1"
                     /translation="MKKVLVIIFIAIVVVALGLVGYYYFFDYTVSPTTGEKESFREFL
                     PFGSNTAGSSATTTADNGEEANGGTSTPSGIPAGPLPQLQQITMSPVAGETMVDAVNA
                     STTVVRYVDRATGNLYDFNVQTGVISRDTNTTMPKIREAVWDPSGAALIMRFLEGDTD
                     FIQSYYAQFDPNSAEADGNRSLTGSYLPASIDSITPLGGGKIFSLMTSTSGSSGYVSS
                     MNGAGRTLVFQSPVREWIPQGIDQNTVALTTKASSYTNGFLYLFNIKTAKQSKIIGNV
                     QGLTTLMSHDGKYVLFGENDNGYKLRMLNVATKAVTALPVNSIPEKCAWSDDDAVVFC
                     AASKSPLNADFLDLWYQGMVSTSDDLWEINPIDGTSIVIADMSGKNIDGVDLVPSSDR
                     TSVAFMNRNDLSLWIYRLNSQ"
     gene            complement(14246..15211)
                     /locus_tag="VHF05_01685"
     CDS             complement(14246..15211)
                     /locus_tag="VHF05_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVS79667.1"
                     /translation="MKRHFYNLLLSATLVALMGIAIVPAFANAQAAASGGGGGLTTDP
                     GNSASYVPLAPLPYADPGPQQTQGLLQKYLPGVMKLAIAITIFLSVLMIMYGGFQYIT
                     STSLGGKSGGRNTINNAVIGLILAFVSWLILYTINPNLVSINLDLTLPAQPQNAVTAG
                     GTTGGTGGITAGGGNGGGAVDPALALSSFNSAGFDTNANCVTQPGCKTSLIGIQKNTV
                     EGANLLKSNCSACTNLVITGGTEPGHTDSGPYTHANGYKLDISDKQGDGAAFSDYMDS
                     TIKAQLGTVPPGNTWVTITQNGYTYKVAHEDIGQSNSHWDIQVNK"
     gene            complement(15221..16201)
                     /locus_tag="VHF05_01690"
     CDS             complement(15221..16201)
                     /locus_tag="VHF05_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79668.1"
                     /translation="MAQTINPARSLNLSGINLSDYGYGDVIDIAANPASPAPGAPVTV
                     VIDSNTTNLDLSQIVWSVNGKEVQNGIGIKSYTFTAGELGQKTTVSVKITTPQGAIDN
                     QSISVTPGEVDMIWQTDTYTPPFYEGKAMYTTQSAVQVTAIPHMLGSNGAEINPANLL
                     YKWTRDGSVIGNQSGYGRNTLTFSDTDLPRETDVSVEVTSYDGTQTSRGFVAITPIPP
                     KVISYEDNPRLGVLSNKALIGDVTMKDQEITLTAYPFFFSAGLNGANMKYEWDMNGQP
                     IDNSGSNHVVLRTTTDEEGNALVDVTAKQNNFILQYADAQTMLYFNNKQN"
     gene            complement(16510..17043)
                     /locus_tag="VHF05_01695"
     CDS             complement(16510..17043)
                     /locus_tag="VHF05_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79669.1"
                     /translation="MFGESKLTGIQIVLIMTVAGLIDLVQFGVSFIPVAGEIISVLLD
                     VAGILIFAIWLMICGISPAEPDVFLVLLAGTVLEAIPVADMLPGWFGTMFYICMKHKI
                     PAVEAVTSIAEGDVGGAVGALKNQAAVMNANRAAAMRDPDLYDDEEQAEERETPRLRP
                     VVQDIRPARKDTAKTTQ"
     gene            complement(17048..18934)
                     /locus_tag="VHF05_01700"
     CDS             complement(17048..18934)
                     /locus_tag="VHF05_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HVS79670.1"
                     /translation="MSLFSNPFKTPEKKASNADLSSFLPKAVYESEALELQDVIAPSA
                     LKITPKELNLGEKIVRTFFVISYPRYLAESWFAPIINLDKVFDISIFVHPIETSKILR
                     TFQKKVAEVQSQIHTREEKGLVRDPMLDTAYQDLENLRDSLQQAQERMFDVGVYISIY
                     ADTTEELDHVESEVRSILESQLVYVKPALFQQEQGYQSVLPLATDLLAVHSKLNSTPL
                     SSLFPFISFDLTSDKGILYGVNRHNSSLILFDRFSMENYNSVTFAKSGSGKSYATKLE
                     ILRSLMFETKVLVIDPEREYEYMAEATGGRYFKISLSSEHHINPFELPAVTEGESAAS
                     VLRSNIVNLVGLFRIMMGGLTAEEDALIDRAITETYALKDITAESNFENIEPPLLSDF
                     ELVLSGMEGGESLAQRLTKYTKGTWAGFINKPTNIDINKKFVVFSIRDMEDELKPVAM
                     YIITHYIWNAIRKSREKRLLVIDEAWWMMKSEDTASFLLSLAKRGRKYYLGLATITQD
                     VEDFLSSPYGTPIVTNSSIQLLMKQSPTSIDKIQQTFNLTDEEKYLLLESDVGEGIFF
                     AGLKHVAIKIIASYTEDQIITSDPSQLLAIKKAKDDLIKSEAESALAPAAPQPIAGEA
                     PLKQ"
     gene            complement(18986..19369)
                     /locus_tag="VHF05_01705"
     CDS             complement(18986..19369)
                     /locus_tag="VHF05_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVS79671.1"
                     /translation="MPQGSTSREYDLEERTAKFGEEIVKFSIRLPRTIVTDPLVSQLV
                     RSATSIGANYMEANGASSKKDFRNKIHICKKEAQESKHWLRMLAIAVPNEADALRILW
                     KECQELTLIFQKIIQTLKKSRLIEN"
     gene            complement(19408..20052)
                     /locus_tag="VHF05_01710"
     CDS             complement(19408..20052)
                     /locus_tag="VHF05_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79672.1"
                     /translation="MAQTALSTQDFVPIKEIRNGIVTLKDGSLRAVVLASSLNFSLKS
                     EDERNAILLQFQEFLNSLDFSIQIFVQSRKLDIRPYLALMEQRYKEQTIDLMKIQIQE
                     YIEFIRNFTESTNIMTKNFFIVIPYIPAMIKSNGGLLSGILPSRGGAKGAAAGSGEEQ
                     FEENRTQLEQRMAVVEQGLVRCGIRVVQLGTEEVTEFFYKIFNPGETDKPIKLS"
     gene            complement(20070..20516)
                     /locus_tag="VHF05_01715"
     CDS             complement(20070..20516)
                     /locus_tag="VHF05_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HVS79673.1"
                     /translation="MRFQVPQFIEVEDKIFGPLTFKQFIYLGGGGGLSVVFYMLLPKF
                     ISFLLILPVVAFSLALAFYKVNNKPFINVVEAFFKYVVGGKLYIWKKQPKKAVAKAVE
                     TPAYSQVYVPKLGDSKLKDLTWSLDVRESVNPVTPNSGEQSKTPQA"
     gene            20743..21387
                     /locus_tag="VHF05_01720"
     CDS             20743..21387
                     /locus_tag="VHF05_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79674.1"
                     /translation="MIYDVIRKGIALSSQQQRRLEMSKNAKKLMSLVAIMGIVVVLSI
                     ALNSNPTNPNKSSRVVSRAAENIIATYQSLPSSEARYGLIHDKIGDVKSSSDAIALCR
                     IIGFHRSFPDKNTKDIQLEAAKKALDLSSNDRTALLELHELVYDPSTADLRNKVLVPL
                     DERLVALTDMKNQTQVNSLCGAWRVEESFKDVARKSRYGNQTLQRKLLDVCMGN"
     gene            21604..22071
                     /locus_tag="VHF05_01725"
     CDS             21604..22071
                     /locus_tag="VHF05_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79675.1"
                     /translation="MSSKTERLIEEYQALPNSEDRYELVHNRISDIKSPADALALCRT
                     IGFRESFPDENTKQIQLEVAKKALEIGFNDRKTLIELHKMVYDPSTAELRKAVLIPLD
                     KQLIKLTDFKNPVQLRSFCDAWKVELTFTKVTDAIQFNARQTLFQYLVEVCNQ"
     gene            complement(22152..22868)
                     /locus_tag="VHF05_01730"
     CDS             complement(22152..22868)
                     /locus_tag="VHF05_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79676.1"
                     /translation="MDISSDDFIARLNTIPPEIDAATSSFGFLAALYEIAGKYHLDDE
                     QAGIMIDLSNLRAIELLKREELAAQIDKIQGIDSETAKKVSNEIETNVMNQLLHFAHG
                     DISTDEFYRIYDAYKKAQPKAEGLAFLDNRLGGEKTDESKLMPMVEKQPAPTQSETPR
                     PTQPQVSIPTAGPNIKVEEIKKPEPATPTFTPPPPQTPAPQPSTAPAAPNSAPQPQPA
                     QPSTPKQYPGGQDPYREPLE"
     gene            complement(22875..25331)
                     /locus_tag="VHF05_01735"
     CDS             complement(22875..25331)
                     /locus_tag="VHF05_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79677.1"
                     /translation="MIVLAGASVSLLPLGVHISHADLVTDWLTNGGAANAAVQAQQVV
                     DPGTGCSLFSFDGGKCFEFLTANAVQIIVEGIGWILTLSGWVLNYSINATIVNMSQNL
                     KDFAGIQSGWRAIRDLGNMIFIFLLLYEAILTVIGRGHYQKTIRNVVIAALLVNFSLF
                     FTQVAIDASNIVTIGFYTSIQQSSLAAGAPTTDPTSFGFSNAFMAPLGLSSFFQATDA
                     AKNLAQSGTMAIILNGLLASILILIASVIFLAMALMLVIRFIVLVFLMMLSPLAVLGI
                     VMPQVGSMSRRWLNTLNGQLLFAPVFMILSWLTLRVISNSPIANTISGKSFTDLAGGG
                     TGALNSTSLLFNYAVIIGFMVFSLVASKNLATRGGGMIANAMNKGSQWAGGAVFGSAG
                     YGLRRSVGKLGQATVDSKWANRLKTTPMLGGAVGWTSRRAFKQVDEKVAKNSFDLRNT
                     GIGKGLGTGSGQKGGFRQWKKGRDDEKRETGEIKKKLETQADIKAGTQAFDARQKLLE
                     EQEKLNATLATATVGTPAHAAATTRLAAIKTEIGNHENTENKARKTIQGLNDKQLEGL
                     GKSLEHEFVAQSLSPQKLNSTMRAEKHDDERKEKVKQARYKPVYEHVKALDDFNSRTA
                     GRALTPAEIDEKEKLEKNAKEAISRLSVQDMEYIDPTHFRNENFAMHVQQGVFEGVTK
                     PEHAALTNTEKTQLAEARLAPVIRALSNPAIPGIKNPNSNYNANDAYDAIFKLDGKAA
                     AKLDLNLLTNPDALEAFDAKKLGDMAKSMDRTKRLAFKNAFNANAAPFIPPAVPTGTH
                     KELEKLVAWLNTGKGKKMYS"
     gene            complement(25371..26753)
                     /locus_tag="VHF05_01740"
     CDS             complement(25371..26753)
                     /locus_tag="VHF05_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79678.1"
                     /translation="MFVFPLGILQPKQAQAQWAVFDIPTEIETTISAFNQFSLVMKEY
                     VGDSVAWFIAKVIIQQMTASVVDWINNGFNGNPAFVQNPQQYFTNIGDAIAGNFILND
                     PSLNFLCSSFGPQLRIGLNNQRMLPFQNSITCTLTNVIQNIDAFTQNFNNGGWLAWNT
                     MVTEPQNNPYGAYLMLTNEYTRRRDQIENSQKLQLDWGKGFLSFTDANGVIQTPGSVI
                     ETQLNQHLSSGLQSLVGADEINEIIGALMTQLVTKTLSSVSGGLRGLGTASGLNDGGA
                     SLQQMQNIQIPSQGTCTGATGGQINPDLSISGGTGSACSSPDANSYLPGFGLPPGSIS
                     QTPPPPPATPPITTPPPAPTEEPTTTPTTTPTTPPPPPATPPTTPPPSNPGGFKVGDT
                     IQAMLATSVRVAANVGAQEISVAAVGDKGTLLEQGPFAGGSAVYWWRIRWANGIEGWS
                     MGSTLGYPIN"
     gene            complement(26845..27855)
                     /locus_tag="VHF05_01745"
     CDS             complement(26845..27855)
                     /locus_tag="VHF05_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004592605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79679.1"
                     /translation="MRAPGKKIFLVLLACILVIAGIYYAYKKTGSKAQNASTPDNLVQ
                     AVFSGQSDTDTDGDGLKDWEEALWRTDPNKADTDGDGTPDGEEIRESRDPLKANTAAN
                     GAPPTDIMASTTVSAALNNAATTTEMTSTDLFARAFFANYVQLKGEGITISSTTEQAL
                     IDETLQSGDYNLPVPKQYAMSDLHVSQDNSSAAYHAYGNKLARIIIDSSPKEKHDDEV
                     TIFKNAVQKNDESALEGLDPIIDGYQKTLDGFLAMNVPSSIATYHLEIVNGISAMLTD
                     QRAMRNLFKDAVPAYARFSDYQNDTYAMLSGLNAVVGYYKQNGIVFEQGEDGYKFMST
                     IQ"
     gene            complement(27931..28749)
                     /gene="rsmA"
                     /locus_tag="VHF05_01750"
     CDS             complement(27931..28749)
                     /gene="rsmA"
                     /locus_tag="VHF05_01750"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVS79680.1"
                     /translation="MPDDLEKNDNKNGLAPKKSLGQNFLTSPAAFARIVDAADIRPGE
                     TVLEIGPGKGGLTERLLEAGGNVTAIEKDARLIPFLREKFAKEIESGALSLLERDALE
                     FDTGEIKSGYKLVANIPYYITGAIIRKFLSSGRKPSAMVLLVQREVAERIAAKDGKES
                     LLSLSIKAYGKPTMAGVVHAGSFFPKPSVDSAILVVRGISDEFFAGINEEDFFKAIHA
                     GFAQKRKMLRRNLEAAFEKSAIERAFEGADLLPTVRAEDLSLEEWGKLVRNLSS"
     gene            complement(28878..>30158)
                     /locus_tag="VHF05_01755"
     CDS             complement(28878..>30158)
                     /locus_tag="VHF05_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVS79681.1"
                     /translation="YQDTNKVQYMIAKLLSEKHRNICVVGDSDQNIYSWRGAQLRNIL
                     NFERDFPGAQVILLERNYRSTKTILAVANGIIKKNKFRNEKNLFTDNVDGEKISLYEA
                     YDEQDEASFVARTAKQIIREGAEPSEIAVLYRANYQSRALEEAFLHGDVPYQVLGVRF
                     FERKEVKDVLSYIKAALNSDSLSDLKRIINVPARGIGKVTLLKVLANQRESLTGAMRE
                     KVEKFYILLSRIRETALHERPSELVRFVLREAGFETLYRQGSEEDQERLENVKELASL
                     AAKYDEFGPEEGLEKFLTDAALASDQDELSETRQGAKLMTVHASKGLEFDYVFITGLE
                     QGLFPSNRAEAEAPTEESEEERRLFYVALTRARRKVFLTHAQIRTIFGSKQVNVPSEF
                     LYDIDENLLETDPGSVNQGDDRADAIRKIFTIDF"
BASE COUNT         6938 a         7711 c         8036 g         7473 t
ORIGIN      
        1 gaaatctgaa tttcgcgaag cggcttcgcc aaaaaacggc ggcattccat ggaatgccgc
       61 cgttttttgg cgaagccgct tcgcgaaatt cagatttcgc gctctcagct tgccttcttc
      121 tcttttctgt ttaataagca ggttcaaatt tgattcccac tccgatacaa aaatcaaccg
      181 gcacgcaagt gccggttgat tcttcttttt ttatgtggaa tagcggattt ttttaatgcg
      241 gggcaccttc tttcatgtat acttattatc agaagtcagc cctctcccat ccaaggcacg
      301 aatctctaag cgacactttt aatataaata ctaaaagcta agcttgactc tcaggatagc
      361 tccagttaat ccgccgaagc ggcaaccagg acgtcctcgc agaaaagctc gtagggcctc
      421 gcagcactag tcgcttagag attcgtattt tggatacgat cctcacgcta tttgccgcgc
      481 ttccttgcgg gaaattcggg caaataaata tctgctcgcg ctttttgcgc cgagcagctg
      541 tataattact gtctgggatg aacttgctgt tacgaaatcc atcctagcac gaaactattt
      601 ttatgtcaag caatactgaa gaggtgcggc aggaggggcc tgattcccga tatctggatc
      661 agactctccg cccgaacacc tggagcgaat acgtggggca ggaagcggtt aaaaagaatc
      721 tcgtcatcct cctttctgcc gcaaaggagc gcagccatcc gcccgaacac atcctttttt
      781 acggaccgcc gggcttgggc aaaacgaccc tggcgcacct catagcgaaa gagatcggcg
      841 cgcaaatgaa aatcacctcc gggcccgcca tcgaaaaggt cggcgatctc gcctcgatcc
      901 tcacaaacct ctccgcggga gacattcttt ttattgacga aatccatcgc ctgaacaagg
      961 cgatcgagga agtcctctac cccgctctcg agtcgggcac cttggatatc atcatcggca
     1021 aggggccgtc ggcgcgcacc atccagctcg atctgccgcc cttcacgctc atcgccgcga
     1081 cgacgcgcat cggcctcatc tcgtccccgt tgcggagccg cttttccggc ggcgtcttca
     1141 ggctcgagtt ctataccatt cccgaaattt ccgagatcgt ccgccgctcg gcgaagatcc
     1201 tcggcgtccc cctcgacgag aaagctgcgc tcgagatctc gaagcggagc cgcttcacgc
     1261 cccggaccgc gaactatctc ctcaagcgga gccgcgattt cgcccaggtc tacaagaagg
     1321 atctctcgaa ggaggtcgtc gagcaggcgc tcgagctcct gggcatcgat gcggtcggcc
     1381 tcaattcctc cgatcgcgcg atcctctcca ccatcatcga aaaattcaac ggcggcccgg
     1441 tggggctcaa caccatttcc gcatcgctct ccgaagacca ggcgactatc gaggaataca
     1501 acgagccgta tctcatccag accggcttcc tcgaccgcac gccgcgcggc cgcgtcgtca
     1561 ccgaaaaagg ctacgaacat atgggccaga cgccgccggc caatctccaa gacaagcttc
     1621 tttagctccg ccggcgcggt tgtcggaaat accggcgcat gatggacaaa tcctttttcc
     1681 ctgggtaatc taaggactgt aaactagcaa ctagaaactt tcgtatgtcc ggacacaata
     1741 aatggtcaaa aataaagcgc caaaaggaga agactgacgc gcagaaatcg aaaatcttcg
     1801 gcaagatggt gcgcgttatc accgtcgagg ccaagaaatc gaacggccaa atgacgccgt
     1861 cgctgcgcgc cgcggtcgac gcggcaaaag cggcgaacgt gccggccgac aacatcgacc
     1921 gcgccatcaa gaaagcgagc gagccgagcg aaaacctcga ctcgatcacc tacgaagcct
     1981 acggcccggg aggatgcgcg ctcataatcg acgctctcac ctcgaaccgc aacaaagcgg
     2041 cccaggaaat caagcatatt ctttccaagc acggattcgc gctcgcagcg ccgggttccg
     2101 cccagtgggc attcaccaag tcagtcgagg aaggctgggt gccgaactcg accgttccca
     2161 tctccgaagc cgacggcgct cttctcgaaa cgctcatgtc cgatctcgag gacaacgacg
     2221 aggtgcagga tgtgtacacc aatgccgaat aagaattcgg cataatttcc aattagcaat
     2281 ttttaatttt caatttcaca atattccaaa gtttgaagtt tgcttcattg gttattattt
     2341 gaaaattgga aattgtaaat tgaaaattta attttcattc cgatgaaaat tttaggcatc
     2401 gatcccggct atgagcgtct cggcatcgcg gtgatcgaaa aggaaaagaa tgcgaaagat
     2461 caggtgctct tttccgagtg cttcaagacc ttaagctcgc ttccccaccc cgagcgcctg
     2521 cggctcctcg gacaggagat tgagcgcgtg atcgaggaat tccggccaga agcgctcggg
     2581 atcgaaacgc tttttttcaa caagaaccag aaaaccgcca tgcacgtttc cgaagcgcga
     2641 ggctccattc tctatgccgc ggcttccaag ggcttggcta tccacgaatt cacgccgggg
     2701 gaaataaaaa tggcactctc cggctacggc aagagcgaca aggatcagat gatcaagatg
     2761 gtgaatctcc tggtcaaagt ccgccccgga atcaaatatg atgatgaata tgacgccatc
     2821 gcggccggtc ttaccctctc ggccacgctg ggcatcaagg ccttaacgaa gccttgaaat
     2881 taatggccgg aggactatcc acagaacgca tttttgagca ttgcaaaaat atatggatct
     2941 gctaaaaata ggctattccg aaagcgcacc gcttgaggaa ctttagaaag attacaaatt
     3001 tattcattca aaatgttcgc agaagaattg aagtacgcat cgccgatcct cgctatggaa
     3061 gatgaggaag aggaagaaga tgaattcaaa gacgacgaag gtgactccga agacgacttc
     3121 tccggtgaag atgaggaaga tgacgatatg gatgaagaag aaggaaccga cgaaggagaa
     3181 ggagaaagct attaattttc tgcactgaaa aaccgccccg tgagatagcg cgaagcgcaa
     3241 catctcacgg ggcggttttt ctttttttat tgcaacgagc gagcggtgaa tgagagtctt
     3301 ctttcattca tgtccgagac gttgcaatat aagggctgat acccacacct tcagggcggc
     3361 gcatgtgcga gtccgcgagt acgtgcgacc gggatttatc ccggagtatg ccgcttttat
     3421 ttaatattga tcttgatgaa tctacgcttg ccgactttga ggataagagg ttcctcgatt
     3481 ttctgatggg catccttgat ctcctcgcct ttttcgaggt cctctatcgc accctcatcg
     3541 agaagcctcc tgaattcggt cttcgaggaa acgatgctcg cgtttaccaa tacctcagca
     3601 aggctcgccc ctttttccgc ggacgtctcg ggaatgttat ccggaatgcc gcctttcttg
     3661 aacgtgctgg aaaagccctc cttggccttt cccgccgcat cctttccgtg atacatggtg
     3721 acgatctgct ctgcaaggag ctccttggcg tcgcgggggt tcatttcgcc cgaatcgatc
     3781 tttctttgca ttttttcgat atcgctgagg ggcatatacg tgcagagctc gaaataatta
     3841 ccgatggagc tgtcgggaat cgacataatc ttgccgaaca tgtcgaacgg ttcgtcgctt
     3901 atggcgacgt aattgccgag gcttttcgac atcttctcct tgccgtcggt tccctcgagg
     3961 agcttgaagg aaaggacggc ctgcggctcc tggccgttca ttttcatgat gtcgcgccca
     4021 aggagcatat tgaaggtctg atcagtgccg ccgacttcga gatcgcacga gccgtagatg
     4081 agattgagca cgtgcgaatc gatgccctgg agcaccgggt acatcatttc gtgcatgaaa
     4141 agctcctcgt tcttttctat ccgctccctg aacatgtcgc gctcgataag acgcgaatgg
     4201 gtaatatggc ggagagtgga gagaatgttc gcgaggctca cgcttaccac atgggctttc
     4261 ttgagatggg tcttctgcat gcgcgtctcg tcgtagagca ccgccttgcc gatgaacgag
     4321 ctcggattga tgaacgtcgt cttgaagcgc acgaggttgc cgggcttcgg ctggatatcg
     4381 gcaacgccga ggaaccagtc ggaattccgg atccaggaga agcggtcttc gccgacaaga
     4441 gtgatctttc cgacctgctc gaggtacgtc atcatgttgc gttcgacttc gcgctggtcg
     4501 atttcggggc ggaccttgct tttgccggtc ggatcgccga tgcgcgcggt aaaatcgccg
     4561 atgaggaaaa tgactttgca gccgagatcc tggaaggcgc gaagcttccg aagcacgacg
     4621 gcatggccga gatggatgtc tgggcgcgtc ggatcgacgc cgaacttgat gatgatgtcc
     4681 ctgtcgtatt ctcccctagc tttctttacg agcttctccc tgaacgctcc gtcggggtcg
     4741 ataaactcgc ccacgccccg ggaaaacaga tcgtctattt ttttgacttt ttcttcgaga
     4801 tccattctgg tggaaatgtg atgctgctcg gaattttgct ccgtttttcg tatccgtgcg
     4861 ggatgatcgg ccttgattgc gcggaatttt gagagtataa ggtatgggtc gttcggaacc
     4921 cgcgcagcgt atttctaatg tagcatattt gaccgccttc cggtatcgcg cgcccgaatc
     4981 cacaaaaatc ttcatatgat acaatttacc aatatgagaa ggaaagttca ttcactttgg
     5041 acgagattta aggaactcgg ctggaagaag atagttacgg taagcgccgg ggtgttcctc
     5101 atccttctgg gcatttttat tatctgggtt tcgactctga agctccctac cctcgactcg
     5161 ttcggggaga gcggcaggct cgccgaatcg actaaaatct acgacaggac ggggcaggtc
     5221 gtgctctatg acgtaaacca gaacctcaag cgcaacgtcg tttccttcga ccagatctcc
     5281 cagcatgtga aggacgcgac gatcgccatc gaggacgaca agttctacga gcaccacggc
     5341 gtccgccccc tttccttcct ccgctccgcc atcgccgacg tcacaagcat gagtttcagc
     5401 cagggaggct cgaccatcac ccagcaagtc atcaaaaaca ccgtcctgtc cggcgaaaag
     5461 accgtgcctc gcaagatcga ggaatggatc ctcgccatca ggctcgaaca gcagatgtcg
     5521 aaaaacgaca ttctcgacat gtatttgaac gtcatcccct atggcggcac catgtacggc
     5581 gtcgaggagg cgagcatgga attcttcggc aagcatgcgg cggacctcga tatcgccgag
     5641 tctgcgtaca ttgcggcgct cccgcagtct cctacctatt actccccata cggcaaccat
     5701 aaggatgcgc tcgaagcgcg taaaaacctc gttcttcaga aaatgctcga gaacggctat
     5761 attacgcagg accaatacga ccaggcgaaa gccgagaagg ttaccttcct tccccaacag
     5821 aatcaaggca tcaaggcgcc gcacttcgtg atgtatatcg aggattatct cgtgcagaaa
     5881 tacggcgagg acgccttgca gcaaggcggc tacaaggtca taaccacgct cgactacgac
     5941 ctgcagtcga aagccgagga ggccgccaag gaatatgccg catcgaacga gaagaatttc
     6001 aatgcttcga atgccgcatt catcgccatc gacccgaaaa ccggccagat cctcgccatg
     6061 gtcggctccc gcgactactt cgatacctcg attgacggca acttcaacgt gacgacggcc
     6121 catcgccagc ctggatcgac cttcaagccg ttcgtatacg cggagagctg gctcaagggc
     6181 tatacgcccg agaccgtgct cttcgacgta ccgacccaat tcagcgtcaa ttgcgctcca
     6241 gacaacatga cggatgataa cggctgctac agcccggtaa actacgatga cggcttccgc
     6301 ggcccgatga cgatgagaaa cgccctcgcc cagtcgatca acgtcccagc cgtcaagacg
     6361 ctctatctcg ccggaatcca ggacaccata aacctcgcgc agtccatggg cgtcggaagc
     6421 ctgggagatc cgaagcaata cggcctcact ctcgccctcg gaggcggtga agtttcgctt
     6481 ctcgacatga cgagcgccta cggcgtattc gcgaatcagg gagtgcgcga gccggccgtc
     6541 ggcattctcg agatcgacga ccgccagggc aacgtgctcg aaaaatttca gccgcacgag
     6601 tcgcaggtgc ttccggccga gattgccaat gagatgaacg atgtgctctc cgatgcggtc
     6661 gcgcgctccc ccgaatacgg ctacaattca gccgtcaata tccccggcca taacgtggca
     6721 gtcaaaaccg gtactacgaa cgattatcgc gacgcatggg tcatcggcta tacgccggac
     6781 atcgtcatcg gcgcatgggc cggcaacaac gacaattccc cgatggtgaa gaagatttcc
     6841 ggattcatcg tggcgccgat gtggcatgtg gtcatgcagt acgcgcttga caggctgccg
     6901 gacgaccaat tcacgcctcc cacgatcgat tacgccggcc taaagcctgt gctgcgcgga
     6961 cagtggcagg gaggagagtc gtattcgatc gatacagttt cgggcaagct ggcgacggcg
     7021 aatactccgc cggaagacgt gcaggagatg tttaccggcg gcatccattc gattctctac
     7081 tgggttaaca aagacgatcc gcgcggcccc attccgacta atccgtcggc cgacgggcag
     7141 tttacaaact gggagtacgc ggtgcaggcc tgggccgcgg cgaacaatat caccagcggc
     7201 actaccactc ccccgacgga cttcgatgac gtacataccg aaggagcggg ccctaagatt
     7261 tccttcgaca atccgacgaa tgacaagcta tacgatcccg actcaaaaat tctcatcagg
     7321 cttaattacg acgagagcgc taatatccaa agggtcgatt actacctcaa tgatcagttc
     7381 atcggctcga gcaccaacga accgttcagt ttctcgttcg ttccgagcga ctatggcgat
     7441 actaaaaaca ccaatgatct ctccgctgtc gcgacggata tgttggggaa tcgcggaacg
     7501 gtgacgatgc gcttcaaggt gaacaagtca aacgcggtta tttcaccccc cgtaatccgg
     7561 ctgtagcggg atcgcttttc aatttctcca ggacgagctg ctttttcaga agcaactcgt
     7621 ttcgcattac cggattgctc ggcacggtag caatcccgtc cttaataact attttcttcg
     7681 gatcgatctc cgctccgatc gactctttca tgatgcgggc aatagcttcg cgctgtgccg
     7741 atccgttcgc gataagagac ttaaatttgc cgaaaaacga agtgatatcc tgcataagga
     7801 tcgaattttt taatttccaa ttttcaatga attttcaagt ttcaatattt caaacatgta
     7861 gatatgtctg ttggtattcc gaaattgatt cattgtgcct tgattgaaaa ttaagagttg
     7921 taaattgaaa attttcaatc atttgttcat cggcatcatt acatacacga acgacggatc
     7981 ggaaacgccc ttgatgacaa taggcctgcc taagccgtta aattggatgg aaaggctctc
     8041 cgaatcgata gactggaaac tgtccgaaaa atacttatag gcgacgttta aggtgatatc
     8101 ctcgccgctc gaagaattct cgatattatt cgtactctcg ccgatatcgg aatttctcgt
     8161 cgtgagctcg aatttcttct tcttaggcga aacattgata ttaagttggt tgaatttgtc
     8221 ggagaagacg ctcgcgagct tcagggaatt catgagatcc tgcttgagga taatcgcctc
     8281 tgtcttgaag cccttcggaa tgatctgctt gtaatcgggg aacgtgccgt caatgacgcg
     8341 ggaaaccaga tagacgttct cgtacgatat agaaagctgg ttcttgctcg cgttcacctc
     8401 gatatcgccc ttggcggcat cgagtatgcg gagaatctcg ggaatattct tcaccggaat
     8461 gagaatatgg ccgaaatccc ttgaaacttt ggcttttacc ctcttttcag aaaccctgaa
     8521 ggagtcggtg gcgacaaata cgatctcttc ctcatccgta tagacataca ccgacgaaag
     8581 ttcgggcttc atgctcgaaa cagcggcgct ccaccatacg gacttgagcc cttttgcgaa
     8641 atcggccgca tcgacggtaa acacctcacc atcctcgacc gtcgggataa tgggaaattc
     8701 ctccgtaggc attgttttga tgaccgtcga gctgttcggt gtggaaaccc tgagattctc
     8761 gccatccgtt tcgacagtca ccatcgaatc gtccggcaga ttggctatga atgagttgaa
     8821 taccgatccc ggaaccgcca cagcgccttc ctcgacgatt gaaatgggaa tatgcatttc
     8881 aaggccgagt tcgagattgg tcgccttgac cgtaagcatc ttattcttcg cctccatgtg
     8941 aatgcatttg agtaccggca gagctgcatt cttctcggcg accttctcag tcctgcttac
     9001 cacctcctgt attttctttt tcggtacttc gatcttcatg tgagtaagta tgtattaatg
     9061 tatatattta aatctattac taatactagg ggtgtgtata tccggataac tcaattaacc
     9121 tttaatatta gcctttattt agcgctttat tcagaataat gtgaggatat aatttgagaa
     9181 tatcttgata aatgctctat tcctggatgt tcatgtggat aaccgcctct cctatccaca
     9241 ccttatctcc aactcgccat aatatccata cacacactat ccacaggttt ttacacaatc
     9301 atcgcccgta tctggctcag ctcctggacc aaggtgctgt cctcgagaag gtcttttttg
     9361 atcttctcgc atgagtggat gacggtcgta tgatccctgt cccccagttt ctggccgatc
     9421 gacggatagg agatgttgaa atcctcgcga aggatgtaca tggtgacttg gcgcggtttt
     9481 atgatctcct tgcggcgggt tttctcatag atgctttcct cgtcgatatt gtagaaatca
     9541 gctataatct tcaccacttc cttgggagaa agggttttct tgggcttggt attgttcttg
     9601 atgaaattct tgatctcaag aaggctcaat tccttgccct tgagctgaga ctgaaagatg
     9661 atcgagttca agaccccctc gagatcgcgg acgttccccg aaatagacga cgcgagatag
     9721 tcgaatactt cgtcggagag gtcgaaactg ttcattctgc actttgcctg aaggatcgcc
     9781 ttcttcgatt cgtgatccgg tgcgggaata tcgatgatca tgccggccga gaagcgcgat
     9841 ttcagccgat cttcgagatt gggtatgaaa ttcggatgct gatcggatga gaagataatt
     9901 tgcttgttgt tgtcgtagag cgtattgaag agatgaaaca gctcctcctg agttttttcc
     9961 gttttcgaga agaactggat gtcgtccatg accaaaatgt cgcattttcg gtatttttcc
    10021 ttgaaattac tcactttatt ggtctgaacc gcattgatat aatcggatgc gaatttctcg
    10081 gaagtggcat aatagacctt cttgttggcc gtatttttga gataattccc tatcgcctga
    10141 atgagatggg ttttgccgtg gccggtgttc ccgtagatga agagcgggtt atagacgatt
    10201 cccggctttt tgacgaccgc ctgggccgcg gcgaatgcga gctcgttaaa aggccccaca
    10261 atgaacgttt cgaaggtgta tcgggggttt aggttatcct ctttgttgat gtaatagtcg
    10321 tttaaaggaa gctctgttgt cggcacggac gcgcttaagc gctcgaatgc gtccttccgg
    10381 gcattcggat cgctcttggt gatagtaaat gcaagcgaat gcacgccgct tgaaaggttc
    10441 cgcaagtgct taagtattac cgagtggtat ttattgtgga gccactcctt tacgaatgcg
    10501 ttcggaaccg aaagaatgac ggtgccgtct tcctgcttca cgataaacgt atccttgaac
    10561 caggtattga agttcggctt tgagacggtc atctcgattt ccgcgagaac gctattccaa
    10621 agagttttgt tgtccatccc gttagaggca gatcccgtta gaaatttcac attcggtcga
    10681 atgctcgttt ccagctttgc tctgcttgaa tttgattagc ttgtaattca tggttcgtag
    10741 atttctaaac gggacagatc gcgctcaccg cgctttccgc aaagttaaaa gaatttcttt
    10801 gtgtaaatat ttcgaccata gttgataaaa taacgcgatc gcggcctcta acgggatcca
    10861 tatcagtttt aaaatttacc ggagtactac tagtatatac tcccgagggt attaaagaac
    10921 ttgcatacag ggcagaggta tgttaataaa ctgttgacaa tctgttgata tggaaagagc
    10981 gggcatattc gaatacatcc gcttgtaaaa ttcaaatttt gcttatataa tcccctatta
    11041 tgtcgtttac ctataatccc aagaagaaaa agagggccaa gacccacggt ttccgctccc
    11101 gcaccaaaag cgcgggcggc cgccggaccc tccgcaacag gcgcaggaag ggacgcgcca
    11161 agctcgcggt ctagagtcta gagagctcca tgcttaaggc agaacaccgg cttaaaagaa
    11221 aatacttcaa ggagatattc gaccgcggcg ctacgtttcg cggtcgatat ttcatattga
    11281 aagtggcaaa ggcggaagac ggcctgccga gcaggttcgg tttttccgta tccaagaagg
    11341 tttacaagtc ggcagtcgat cgcaatagga cgaggcggca gggctacagc gcccttaaaa
    11401 atcatattga tgccgtaaac gggggaatta tcgggctttt ttcgatgatt cgtacgggaa
    11461 aacgcccgaa attcgccgaa atctctcagg atatcgaagc tcttttgagg caatcgcgcc
    11521 ttatttgaag cccttaagaa ggaaaaggga ggaagctggg caatttatcc gaaataagct
    11581 acactgagtc aatgatttgg tttttccaag ccgttttcta caaaccgctc tacaacggtc
    11641 tcattcttct cttgaacgcc attcccggag cggacatcgg cattgcagtc attgtcttca
    11701 cgatcatcgt gaagctcatc ctcttccctc tcacctataa atcgatcaag acccagatcc
    11761 agatgaagcg gttcgagccg gaactcgcga aaatcaggga aaaatacacc gacaagcagg
    11821 aacaggcgct caaggtgatg gaagtctaca agcaggcggg catcaatccg ttcgcgagct
    11881 tctttctcgt ccttattcag ctcccgatcc tcatcagcct ctactacgtc ttcctccatt
    11941 ccggcctgcc gaccatcaat ccggacctta tctatccgtt cgtccatgca aataggccga
    12001 ttgcaatgga acttttcggc ctcatggatc tctcgaagag cagtattgtg atttcccttc
    12061 tcgccgcggt cgccaccttc ttccagatga aatattccgc ctccaccatg ggcagtacgg
    12121 gagtgcgcaa gccgaatgcg agctttaccg acgacctggc ccgcaccatg accacccaaa
    12181 tgaagtacgt ctatcccgtc tttattttca tcatcgccta ccgcctttcg ggcgcggtcg
    12241 ccctctattg gctcgtgaac actctcttta cgctcgccca ggagctctac gcgagaagga
    12301 ggatcggcag gaatcatcct ccggaggcag aagtagcgaa gtaaaaaaac tcaatggata
    12361 cagcgggcat catatcggtc atcggaaaga ctcttgatct ccttggaatc gagtatgagg
    12421 cgatcgaaga gcatccgtcc aagaatgatg ccgtgcggat caatatcttg acccggcagt
    12481 ccggaaggct tatcggcacc cgcggcgaaa cgctctccgc cctcaacctt atcgtcaaga
    12541 aaatggtttc ctcggccaat gaatcgacgc atatttccgt cgacgtgaac ggctatcttg
    12601 aagcggtgga tcaggcgata cggaacaaga cgtacatact cgcggaacgg gcaaaatcct
    12661 tcaagaccga cgtggaaatg gagccgatga gcgcctacga ccgcatggtg gcgcactcct
    12721 ttctcaagga cgatcctcat cttgaaacgg tatcgatcgg cgcgggaaaa aaccgtcgca
    12781 tagtgatcaa atataaggaa taggagcggc aaaaaatttt caattcttaa ttttcaattt
    12841 tcaatcaaaa atcaatgaat caattcctgc ctgccggcag gttcaaatgt ctgaatcatt
    12901 gaaacattga aaattcaatg gaaattgaaa attgcaaatt ccctatctta ctatcagtgt
    12961 atcaatattg aaatacggaa tacaaaggga ataccaactg atcattggga gttcagccgg
    13021 taaatccaga gcgacaagtc gttgcggttc atgaatgcga ccgatgtcct gtcgctcgag
    13081 ggcacgaggt ccacgccgtc gatatttttg cccgacatgt cggcgatgac gatcgacgtg
    13141 ccgtcgatcg gattgatctc ccagaggtcg tccgatgtcg agaccatccc ctggtaccac
    13201 aggtcgagga agtccgcgtt caaggggctc ttcgaggcgg cgcagaagac gaccgcgtca
    13261 tcgtcgctcc aggcgcattt ctcgggaatg gagttcacgg gaagcgccgt gaccgccttc
    13321 gttgcgacgt tgagcatcct gagtttgtag ccgttgtcgt tctcgccgaa gaggacgtat
    13381 ttgccgtcgt ggctcatgag cgttgtgagg ccttggacgt tcccgataat cttgctctgc
    13441 tttgcggtct tgatgttgaa aagatagagg aagccgtttg tgtaggatga ggccttcgtg
    13501 gtgagcgcga cggtattctg gtcgattccc tggggaatcc attcccgcac cggcgactgg
    13561 aagacgagag ttcttccggc tccgttcatg ctcgagacgt agccgctcga gccggaggtg
    13621 cttgtcatga gactgaagat ttttccgccg ccgagcggcg tgatggaatc gatgcttgcg
    13681 gggagatatg agcccgtaag cgaccggttg ccgtccgctt ccgcggaatt gggatcgaat
    13741 tgcgcgtagt agctctggat gaagtcagta tccccttcca ggaagcgcat gataagcgcc
    13801 gccccgctcg ggtcccacac cgcttcgcgg attttcggca tggtggtgtt ggtgtcgcgg
    13861 gaaatgactc cagtttggac attaaaatca tagagattgc cggtcgcgcg gtcgacatag
    13921 cgcaccaccg tcgtcgatgc attcaccgca tcgaccattg tctcgcccgc tacgggcgac
    13981 atggtgatct gctggagctg cggaagaggc ccggcgggaa tccccgaagg agtcgatgtg
    14041 ccgccattcg cttcttcccc gttatctgcc gtcgtcgtcg cggatgatcc tgcagtattc
    14101 gatccgaacg gcaggaattc cctgaatgac tccttttctc cggtcgtcgg gcttaccgtg
    14161 tagtcgaaga aatagtagta gccgacgagg ccgagcgcta ctacgacgat cgctatgaag
    14221 atgataacga gaaccttttt catgcctact tgtttacttg aatatcccag tggctgttcg
    14281 attggcctat atcttcatgt gcgactttat acgtgtagcc gttttgggta atcgtgaccc
    14341 atgtattgcc gggcggaacc gtcccaagct gtgccttgat cgttgaatcc atgtaatccg
    14401 aaaaggccgc gccatcaccc tgtttatcgg aaatatcgag tttgtaaccg ttggcgtgag
    14461 tatagggtcc ggagtcggta tgccccggtt cggtgccgcc cgttatgacg agattagtgc
    14521 atgccgaaca attggatttg agcagattgg cgccctcaac cgtatttttt tggatgccaa
    14581 tgagagatgt tttgcagccc ggctgggtaa cgcaattggc attcgtatca aatcctgcgc
    14641 tgttaaagga ggaaagcgct aaagcgggat cgaccgctcc gcccccattg cctcctccag
    14701 ccgtgattcc tccggtgcct ccggtcgttc cgccggcggt cacggcattt tgaggctgtg
    14761 ccggcaaagt aaggtcgaga tttatcgaga cgaggttcgg gttgatggtg tagaggatga
    14821 gccaggatac gaacgccaaa ataaggccga tcactgcgtt attgatcgta ttgcggccgc
    14881 ccgacttgcc gcccaaggag gtcgaggtga tgtactggaa gccgccgtac atgatcatga
    14941 ggacggagag gaagatggtg atcgcgatgg cgagcttcat cacgcccgga agatacttct
    15001 gcagaaggcc ttgcgtctgc tgagggccgg gatctgcgta cgggagcggc gcaagcggca
    15061 cgtatgaagc ggaatttccc gggtcggtgg taaggccgcc gcctccgccg cttgccgcgg
    15121 cttgggcatt cgcaaaggcg ggaacgatgg cgattcccat gagagccacg agggtggcgg
    15181 agaggagaag gttgtaaaaa tgtcgcttca tgataattgg ttagttttgc ttgttattga
    15241 aatagagcat cgtctgggca tccgcgtact ggaggatgaa attgttctgc ttggcggtaa
    15301 cgtccaccaa ggcattcccc tcttcgtcgg tggtcgttcg gagtacgaca tggttcgagc
    15361 cgctgttgtc gatcggctgg ccgttcatgt cccattcgta tttcatgttc gcgccgttga
    15421 ggccggccga gaagaagaac ggataggcgg tgagcgtgat ctcctggtct ttcatggtca
    15481 cgtcgccgat gagcgccttg ttcgaaagaa cgccgagccg cggattgtcc tcgtaggaaa
    15541 tgaccttggg cggaatggga gtgatcgcca cgaagccgcg ggacgtctgg gttccgtcgt
    15601 agctcgtcac ttcgacggag acgtcggttt cccgcggcag gtcggtgtca ctgaaggtga
    15661 gggtgtttct gccgtagccc gactggttgc ctattacgct tccgtccctc gtccatttat
    15721 agagaagatt tgcgggattg atctccgcgc cgttcgagcc gagcatgtgc gggatggccg
    15781 tcacctggac ggcgctctgc gtggtgtaca tagcctttcc ttcgtagaag ggcggcgtgt
    15841 aggtgtcggt ctgccagatc atgtcgactt ccccgggcgt cacggagatc gactggttgt
    15901 cgatggcgcc ctgcggcgtg gttatcttga cgctcaccgt ggtcttctgg ccgagctctc
    15961 ccgccgtgaa ggtataggat ttgatgccga tgccgttctg cacttccttg ccgttcacgc
    16021 tccagacgat ctgcgaaagg tcgagattcg tagtgttgct gtcgatgact accgtgacgg
    16081 gcgctcccgg ggcggggctg gcggggttcg cggcgatgtc gatgacgtcg ccgtagccgt
    16141 aatcggagag gttgatgccg gagagattga gcgaccgtgc cggattgatg gtttgggcca
    16201 tggcaaagtg agtcgcgcaa agcgcgccaa ccgcaagtag aattaatgat ccccttctca
    16261 ttaaaagcga acgtaaaagc ccttataccg aaaaattata ccatagaatc ctggccggaa
    16321 atggtcgagt taacgcacag aggggttaat tttcaatttg caattctcaa ttttcactga
    16381 atgtctcaat gaaataattt tcaaacatca acaaccagcc agtggttcat tgggatattg
    16441 aaaatttatt gaaaattgca aattgaaaat taaacagccc tgtacgagca aggaaggatt
    16501 ttcctacttt tattgcgtcg tttttgcagt atccttccgt gcgggccgga tatcctgcac
    16561 gaccggccgc aggcgcggcg tttcgcgctc ttccgcctgc tcttcatcgt cataaaggtc
    16621 cggatcgcgc atcgcggccg cgcggttcgc gttcatgacg gctgcttggt tcttcaaggc
    16681 gccgaccgca cctcccacat cgccttccgc gatgctggta accgcttcca ctgcgggaat
    16741 tttgtgcttc atgcatatgt aaaacatcgt accgaaccaa cctggcagca tgtcggcaac
    16801 agggattgct tcaagaaccg ttccggcaag gaggacgaga aaaacatccg gttcggcggg
    16861 actgattccg cagatcatga gccatatggc gaatatgaga atacccgcga catcgagaag
    16921 gacgcttatg atctctccgg caacaggaat aaacgacacg ccgaactgca ctaaatcaat
    16981 aaggcctgcg accgtcatta taaggacgat ctgtattcct gtgagcttgc tttccccgaa
    17041 cataacgtta ttgcttaagc ggcgcctctc ccgcgatcgg ttgaggggcg gccggagcca
    17101 gcgcgctctc agcctccgat tttataaggt catccttcgc cttcttgatg gcgaggagct
    17161 gggacgggtc ggaggtgatg atctggtcct cggtgtatga ggcgatgatc ttgatcgcca
    17221 cgtgcttgag tcccgcaaag aaaatgcctt ccccgacatc cgactcgagc aggaggtatt
    17281 tctcctcgtc ggtgagattg aaggtctgct ggatcttgtc gatcgaggtc ggcgactgct
    17341 tcatcagaag ctggatcgac gagttggtga cgatcggcgt gccatagggc gaggaaagga
    17401 agtcctcgac gtcctgggtg atggttgcga gccccaaata gtacttgcgg ccgcgcttgg
    17461 cgagcgagag gaggaaggaa gcggtgtcct ccgacttcat catccaccac gcctcgtcga
    17521 tgacgagaag gcgcttttcg cggctcttcc tgatggcgtt ccagatgtaa tgggtgatga
    17581 tatacatcgc cacgggcttc agctcgtcct ccatgtcgcg gatcgagaag acgacgaact
    17641 tcttattgat atcgatatta gtcggcttgt tgatgaagcc cgcccaagtg cctttggtgt
    17701 acttggtgag gcgctgagcc agggactcgc cgccctccat tccggagagc acgagctcga
    17761 agtcggagag gagcggcggc tcgatgttct cgaagttgga ttccgcggtg atatccttca
    17821 atgcataggt ttcggtgatg gcgcggtcga taagcgcgtc ctcctcggcc gtgaggccgc
    17881 ccatcatgat gcggaagagg ccgaccaggt tcacgatgtt cgaacgcaaa acgctcgccg
    17941 ccgattcccc ttccgtcacc gccggaagct cgaaaggatt gatgtggtgc tcggacgaga
    18001 gggagatctt gaagtagcgg ccgcccgtcg cttcggccat gtactcgtac tcgcgctcgg
    18061 ggtcgataac gaggactttc gtctcgaaca tgaggctgcg gagaatctca agcttggtgg
    18121 cgtacgattt tcccgaaccg gacttcgcga aggtgacgga attgtagttt tccatcgaga
    18181 agcggtcgaa gaggatgagg gacgaattgt ggcggttgac gccgtagagg atgcccttgt
    18241 cggaggtgag atcgaaggag atgaacggga aaagcgatga gagcggggtg gaattgagct
    18301 tcgagtggac ggcaaggagg tcggtcgcga gcggcaggac gctttggtag ccctgctcct
    18361 gctggaagag cgccggcttc acgtagacga gctgggactc gaggatcgag cgcacttccg
    18421 actcgacatg gtcgagctcc tcagtcgtgt cggcatagat cgagatgtag acgccgacgt
    18481 cgaacatcct ttcctgcgcc tgctgaaggc tgtcacggag attctcgagg tcttggtagg
    18541 cggtatcgag catcggatcg cgcacgaggc ccttctcctc cctggtgtgg atctggcttt
    18601 gcacttcggc cacttttttc tggaaggtgc ggaggatttt cgaggtttcg atcggatgga
    18661 cgaagatcga gatgtcgaag accttgtcga gattgatgat cggcgcgaac cagctttcgg
    18721 cgagatagcg cggatatgaa atgacgaaga aggttcggac gatcttctcg ccgaggttca
    18781 actccttggg agtgatcttg agcgccgagg gcgcgatgac atcctgaagc tcgagcgctt
    18841 cggattcata cacggccttg ggaaggaacg acgagagatc ggcattactg gcctttttct
    18901 ccggtgtttt gaaaggattg ctaaagagac tcatatgttt attgtataat tttcaatttt
    18961 taatttccat tgaattttca atggattaat tttcaatcag ccgtgatttt ttgagtgtct
    19021 gtataatctt ttggaaaata agcgtcaatt cctggcattc tttccaaaga atccgcaaag
    19081 cgtccgcctc gttggggacc gcaatggcga gcattcgaag ccaatgcttg ctttcttgcg
    19141 cctctttctt gcaaatgtgt attttattgc ggaaatcctt tttcgagctg gcgccgttcg
    19201 cttccatgta attcgcgcct atgctcgtag ctgacctgac aagttggctg acaagagggt
    19261 cggtaacgat ggtgcgcggc aatcttattg aaaattttac aatttcttct ccgaacttcg
    19321 ctgtccgttc ctcaaggtca tattctcttg aagtggatcc ttgaggcatt gaaaattgat
    19381 tgaaaattgt aaattaaaaa ttgattatca actcaatttt ataggtttat cggtttctcc
    19441 ggggttgaag atcttgtaga agaattcggt aacttcctcg gtgccgagct ggacgacgcg
    19501 gatgccgcag cgcaccaatc cctgctcgac gaccgccatg cgttgctcaa gctgggtgcg
    19561 gttctcctcg aactgctctt ccccgctccc cgcggccgcg cccttggctc ctcctctgga
    19621 cggcaggatg ccgctcaaaa ggccgccgtt cgatttgatc atcgcgggaa tgtacggaat
    19681 gacgatgaag aagtttttcg tcatgatgtt ggtgctctcg gtgaagttgc ggatgaactc
    19741 gatgtattcc tggatctgga tcttcatgag atcgatcgtc tgctccttgt agcgctgttc
    19801 catgagcgcc aagtacggcc tgatgtcgag cttcctcgac tggacgaaga tctggatcga
    19861 gaaatcgagc gaattgagga attcctggaa ctggaggagg atggcgttgc gttcgtcctc
    19921 tgacttgagc gagaagttga gggaggacgc cagaacgacc gcgcggagcg agccgtcctt
    19981 cagggtgact atgccgttcc tgatctcctt gatcggaacg aaatcctggg tcgaaagagc
    20041 cgtttgtgcc atgatgcgaa taaattatat cacgcctgcg gggtcttcga ttgctccccc
    20101 gaattcggcg ttaccgggtt gacgctttcc ctcacgtcga ggctccacgt gaggtctttg
    20161 agcttgctgt cgccgagctt cgggacgtag acctgcgaat atgccggggt ctcgacggcc
    20221 ttggcgacgg ctttcttcgg ctgcttcttc cagatataga gctttccgcc gaccacatat
    20281 ttgaaaaagg cttcgaccac gttgatgaac ggcttgttgt tcaccttgta gaaggctagc
    20341 gcgagggaaa aagcgacaac ggggagaatg agaaggaagg aaatgaattt cggcaggagc
    20401 atatagaaga ctacggagag acctcccccg ccgccgagat agatgaactg cttgaaggta
    20461 agcgggccga agatcttgtc ctcgacctcg atgaactgag gaacttggaa tcgcatcccg
    20521 ttagagatcg gaaacagccg ctgtttccga catcgtcttc tgatttgtaa gtaagtcgac
    20581 cataactttt cttgtccttg cgttagaaaa tcacaatcaa aatatctcta acgggacgca
    20641 tacgtatagt atagtcgaca tccttccggc ggacaacgaa aaaagggctt cataaaggta
    20701 taaaacctat ccccgctctc ttctcttgac atttacaaaa aaatgatata tgatgtaatt
    20761 agaaagggca ttgccctcag ttctcaacaa caaaggagac ttgaaatgtc gaaaaatgcc
    20821 aagaagttaa tgtctttggt agcgattatg ggcattgtgg ttgtgctttc cattgccctt
    20881 aattcgaacc ctactaatcc taacaaatca tctcgggtag tatcacgagc tgcggaaaat
    20941 attatcgcaa cgtatcagtc gcttccttca agcgaagctc ggtatgggct gattcacgac
    21001 aagatcggcg atgttaaatc atccagcgac gccattgctt tgtgcaggat aatcggcttc
    21061 catagaagct tcccggacaa aaacaccaaa gatatacagt tggaagctgc aaaaaaggct
    21121 cttgatctga gttccaacga ccgaacagct cttcttgagc tacacgaact agtctacgac
    21181 ccatcaactg cagacttgcg caataaggtc ctcgtaccat tagatgagcg tcttgttgcg
    21241 cttaccgaca tgaagaatca gacacaagtc aattcattat gcggagcctg gagagtggag
    21301 gagtcattta aagatgtagc gcggaagtcg cgctacggaa accagaccct acagcgaaag
    21361 ctgttagatg tctgcatggg aaattaaaat ggataaaata gcctgtcacc agaacattgg
    21421 gtggcaggcc tctttttaat gtagataatt gactttttta taaatttata ttattaattc
    21481 ctacggaaaa ggaggcagta cttgaaacat tcagcgaagt atatcattgg aatccttacg
    21541 gcttttttgt tgataggaag tgtggattgt tctcaacaga caactacccg ctccgatgat
    21601 ggggtgtctt caaaaacaga aagattgatt gaggaatatc aggcacttcc taatagtgaa
    21661 gatcgatatg agctggttca caataggatc agtgacatca aatcacccgc tgatgccctc
    21721 gccctgtgcc ggacaatcgg cttcagggag agcttcccgg acgaaaacac caaacaaata
    21781 cagttggaag ttgcgaaaaa agcccttgaa ataggcttca acgatcgaaa aacccttatc
    21841 gaactgcaca agatggtgta tgatccgtcc acagcagagt tgcgcaaggc ggtgctcata
    21901 ccactggata agcaacttat taagctcacg gattttaaga atccggtgca actcaggtct
    21961 ttctgtgatg cgtggaaggt ggagcttact ttcacaaagg taacagacgc gatacaattc
    22021 aacgctcggc aaacattgtt tcaatatcta gttgaggttt gcaatcaata gctatgaggc
    22081 ctgataccga ataattcggt ggcaggcctc tttttaaaaa actctttcac gaacatgaaa
    22141 gagcttttcc tttactcgag cggttcgcgg tacgggtctt ggccgccggg atattgcttg
    22201 ggagtcgatg gctgtgctgg ttgaggctga ggagcggagt tgggtgccgc tggagccgtg
    22261 gagggttgcg gtgcaggagt ttgaggcggg ggaggggtaa aggtaggagt tgcaggctcg
    22321 ggcttcttga tttcctccac cttgatattc ggtcccgcgg tcggaatcga aacctgaggc
    22381 tgggtcggac gaggtgtttc ggattgagtc ggcgccggtt gcttctctac catcggcata
    22441 agcttgcttt catcagtctt ttccccgccg agcctgttgt cgagaaaggc caatccttcc
    22501 gcttttggct gggccttttt atacgcatcg tagatcctat agaactcatc ggtcgatata
    22561 tccccgtggg cgaaatgcag gagctgattc atgacatttg tctcgatctc gtttgagacc
    22621 ttttttgcgg tttccgaatc aattccctga atcttgtcga tttgcgcggc aagttcctcg
    22681 cgcttcagga gctcgattgc gcgcagattg ctgaggtcga tcatgatgcc tgcctgttcg
    22741 tcatcaaggt ggtatttccc tgctatttca tacagggcgg caagaaatcc gaacgacgac
    22801 gtggccgcat cgatctccgg ggggatcgta tttaaccgtg caatgaaatc atcggatgag
    22861 atgtccatag tagtttatga atacattttc ttgcccttgc ccgtgttgag ccacgcaacc
    22921 aacttctcaa gctccttgtg ggtgccggtt ggaactgcgg gaggaataaa cggcgcagca
    22981 ttagcgttga aggcattttt gaatgcaagg cgcttcgttc tatccatact ttttgccata
    23041 tcgccgagct tcttggcatc aaaagcctca agagcatcag gattggtgag cagattgaga
    23101 tcgagttttg cggcagcctt gccatcaagt ttgaagatcg cgtcatatgc atcattggca
    23161 ttgtaattgg aattgggatt ctttatgcct ggaattgcag gatttgaaag cgctctgata
    23221 acaggtgcca gtcgagcttc ggcaagctga gtcttctcgg tgtttgtaag ggcggcatgt
    23281 tcaggcttcg tgactccttc aaatacgcct tgttgcacat gcatagcgaa gttctcgttc
    23341 ctgaagtgag tcggatcgat gtactccata tcctgaacac tgagcctcga gatcgcttcc
    23401 ttggcgtttt tctcaagctt ctccttctcg tcaatttcag ctggtgtgag tgcccttcca
    23461 gccgttctcg aattgaagtc atcgagtgcc ttaacatgct catagaccgg cttatagcga
    23521 gcttgcttga ccttctcttt acgctcgtcg tcatgtttct cggcgcgcat cgtgctgttg
    23581 agtttttgag gtgaaaggct ttgagcgacg aattcatgct caagactctt gccgaggcct
    23641 tcaagctgct tgtcgttaag gccttgaatg gttttacgtg ccttattttc cgtattctcg
    23701 tggttgccaa tttcagtttt aatcgcagca agacgagtgg ttgccgccgc atgcgcggga
    23761 gttcccacag tagctgtagc aagtgttgca ttgagctttt cctgttcctc cagaagtttc
    23821 tggcgagcgt cgaatgcctg agtgccagcc ttaatgtcag cctgagtctc aagctttttc
    23881 ttgatctccc cggtctcccg cttttcatcg tctcttcctt tcttccactg cctgaagccg
    23941 cctttctggc cgctgccggt accgagacct ttgccgatac cggtgttgcg aaggtcgaag
    24001 ctgtttttcg ccactttctc atcgacttgt ttgaaggcgc gcctgctggt ccaaccgact
    24061 gctccaccca acattggagt agtcttcagg cgatttgccc acttggaatc cactgtagct
    24121 tgaccgagct tgccaactga acgccgtaat ccatatcctg cacttccaaa aactgctcct
    24181 cccgcccact ggctgccctt attcatagcg ttggcgatca ttccgccgcc gcgcgtggcg
    24241 agatttttcg atgccacaag agaaaagacc atgaagccaa taatgactgc atagttgaag
    24301 aggaggcttg tgctgttgag ggctccagtc cctcctcctg ccagatcagt aaaactctta
    24361 ccggaaattg tatttgcaat cggtgagttg gatatgacac gaagagtcag ccagctcagt
    24421 atcatgaaca ctggagcaaa aagaagctgg ccgttcagtg tgttaagcca gcgtctcgac
    24481 atgcttccta cttgcggcat cacaatgccg agaaccgcca aaggagagag catcatgagg
    24541 aagacaagga cgataaatcg gataaccagc ataagggcca tcgcaagaaa gatcactgat
    24601 gcaataagaa tgagaattga agccaataaa ccattcaaaa tgattgccat cgtgccactt
    24661 tgcgccaagt ttttagctgc atcggttgct tggaagaagg aagaaaggcc taaaggcgcc
    24721 atgaacgcat tcgaaaaacc aaaacttgtg ggatctgtag ttggagcgcc tgccgctaga
    24781 ctgctttgct ggattgatgt gtagaagccg atagtgacaa tattcgatgc atcgattgca
    24841 acctgagtga agaagaggct gaaattgacg agaagggccg caatgacaac gttgcggatc
    24901 gttttctggt aatggcccct gccgatcacg gtgagaatgg cttcatagag gaggaggaag
    24961 atgaaaatca tattgccgag gtcgcggatg gctcgccagc cagactggat gcctgcgaaa
    25021 tccttgagat tctgactcat attgacgatc gtcgcgttga ttgagtagtt gagaacccag
    25081 ccagagagtg taagaatcca gccaataccc tcgacaatta tctgcactgc gttagcagtg
    25141 agaaattcaa aacattttcc accgtcaaaa ctaaaaagcg aacaccctgt tcccgggtcg
    25201 acaacctgtt gagcttgaac tgctgcgttc gccgccccac cattagtaag ccagtcagta
    25261 acaagatcag catgggatat atgaacgcca agaggcagga gtgatacgct cgctcctgcc
    25321 aggacgatca ttcccgctat ggcaaaattt cgaaaaagcc tcttcatgta ctaattgatc
    25381 ggatatccca aggtgcttcc catggaccac ccttcgatgc cgtttgccca gcggattctc
    25441 caccagtaga cggcagatcc tccggcaaac ggcccttgtt cgagaagggt tcccttgtcc
    25501 ccgactgccg ctaccgagat ttcctgcgcc ccgacgttcg cggcgactcg cacagatgtc
    25561 gcaagcatcg cctggatcgt gtcgcctacc ttgaacccgc ctgggtttga aggaggaggt
    25621 gtggtgggcg gagttgcagg cggtggcgga ggcgttgtcg gagtggtagt aggagtcgtt
    25681 gttggctctt ccgtgggtgc cgggggagga gtcgtgatgg gaggcgttgc cggcggagga
    25741 ggcggagtct gagaaatcga ccctggcggc aggccgaagc cggggagata ggaatttgca
    25801 tccggagaac tgcaggcgga gcctgtaccg cccgagatgg aaagatccgg attgatctgc
    25861 ccgccagtcg cgccggtgca tgtgccctgg gaaggaatct ggatattctg catctgctgg
    25921 agcgacgcgc cgccgtcatt gagaccggaa gcggtaccga gcccgcggag gccgccggag
    25981 acggagctca gggttttggt cacgagctga gtcatgagcg ctccgatgat ctcattgatc
    26041 tcatcagcgc caacgaggct ctgcaagccg ctggagagat gttggttcag ctgtgtttcg
    26101 ataaccgaac cgggagtttg aatgacacca tttgcatctg tgaaactcaa aaatccctta
    26161 ccccagtcca actggagttt ttgcgaattc tcaatctgat cgcgtcttct tgtatattcg
    26221 tttgtaagca taagatatgc gccgtatgga ttgttctgtg gctccgttac catcgtgttc
    26281 cacgcaagcc agccgccatt attgaaattt tgggtgaagg catcgatgtt ctggatgacg
    26341 ttggtgaggg tacaggtaat agaattttgg aagggcagca ttcgctgatt attaaggccg
    26401 atacgcaact gagggccgaa agaactgcag aggaaattga gacttggatc gtttagaata
    26461 aaattacctg cgattgcatc tcctatatta gtgaaatact gttggggatt ctgcacaaat
    26521 gcgggattgc cgttgaatcc gttgttgatc cagtcgacga cgcttgccgt catctgctgg
    26581 atgatgactt tggcgatgaa ccatgccacg gagtctccca catactcttt cataacgagg
    26641 gagaattgat tgaaggcgct gatcgtcgtc tcgatttccg tcgggatatc gaatacggcc
    26701 cattgcgcct gcgcttgctt cggctggaga atgccgagcg ggaaaacgaa cacgcagatg
    26761 aaaatcgcga ttatgagctt acgatgaaga ccgtgagatt tttttttcaa ttcacgaagc
    26821 attgcacggc tctaaacctt gtaattattg tatcgtagac atgaacttat atccatcctc
    26881 gccttgttca aaaacgatgc cgttctgctt gtagtagccg accacggcgt tcaagccgct
    26941 cagcatcgca tacgtatcat tctgataatc gctgaagcgc gcgtaggccg gtacggcatc
    27001 cttgaagaga ttgcgcatcg ctctctggtc ggtcagcatg gcgctgatgc cgttcacgat
    27061 ttccaggtgg taggtcgcga tactcgacgg tacgttcatg gcgagaaaac cgtcgagcgt
    27121 tttttgatag ccgtcgatga tcgggtcgag cccttcgagc gcggattcgt cgtttttctg
    27181 gaccgcgttc ttgaatatcg tgacttcatc atcgtgcttc tctttcggcg agctgtcgat
    27241 gataatgcgg gcgagcttgt tgccgtaggc atgatatgcg gccgagctgt tatcctggct
    27301 tacatgaagg tcgctcatcg cgtattgctt cggcaccggc aggttgtaat cgccgctctg
    27361 gagcgtttcg tcgatgagag cctgctcggt cgtcgagcta atggtgatgc cttcgccctt
    27421 gagctgtacg taatttgcga aaaacgcgcg ggcgaagagg tcggtcgacg tcatctctgt
    27481 ggtcgtggcg gcgttgttga gggcagcgga aacggttgtc gatgccatga tgtcggtggg
    27541 cggagcgcca tttgccgccg tattggcctt gagcggatcg cggctctccc tgatctcttc
    27601 cccgtcagga gtgccgtcgc cgtcggtgtc cgccttgttc gggtcggttc tccagagcgc
    27661 ttcttcccaa tccttgagcc cgtcgccgtc ggtgtccgtg tcgctttggc ccgagaatac
    27721 cgcctgaacg agattgtcgg gcgtgctcgc attctgcgct ttgctccccg tcttcttgta
    27781 cgcataatag atgcctgcga tcacgagaat gcaagcgagg agtacgagaa aaattttttt
    27841 gccgggagcg cgcatgaaag gaatatgaat attatatacc agtttccact gctccacagc
    27901 ttcagattat ggaaaatccc gcctggaagt tcatgaagag agatttcgaa cgagttttcc
    27961 ccattcctcg agcgaaaggt cttccgcacg gactgtcgga agaagatccg ctccctcgaa
    28021 agccctctca atcgccgatt tttcaaatgc cgcttcgaga tttcgcctga gcatcttgcg
    28081 cttctgggcg aatccggcgt gaattgcctt gaaaaaatct tcttcattga tgccggcaaa
    28141 aaattcatcc gaaatgccgc gtacgacaag gatcgccgaa tcaaccgacg gcttcgggaa
    28201 aaacgagcct gcgtggacca cgcctgccat cgtgggcttc ccgtatgcct tgatcgagag
    28261 tgagaggagg ctttccttgc cgtctttcgc cgcgatgcgc tccgcgactt cgcgctggac
    28321 gaggaggacc atggcggagg gcttccgtcc ggacgagagg aatttccgga tgatggcgcc
    28381 tgtaatgtag tacggaatgt tggcgacgag cttgtagccg ctctttattt cccccgtatc
    28441 gaactccaga gcgtctcttt caagcagtga aagcgcgcca ctctctattt ctttggcgaa
    28501 tttttcccgg agaaacggaa tgagccgcgc atccttttcg atcgcggtaa cgttccctcc
    28561 ggcttcgagg agacgctccg tcagtccgcc cttgcccggg ccgatttcaa gaaccgtttc
    28621 gcctggccgg atgtccgccg catcgacaat gcgcgcgaat gcggcgggag acgtcaaaaa
    28681 attctggcca agggacttct tgggagcaag gccgttttta ttatcatttt tttcgagatc
    28741 gtcgggcatt acattaaatt ttcaatttcc aattttcaat tttcaatcaa atcacaatga
    28801 attaattttc aaacgtttga atcatcggaa cattgaaaat tcattgtaaa ttgagaattg
    28861 aaaattttta attcctatca aaaatcaatt gtgaagatct tccgtatcgc gtctgcccga
    28921 tcgtctccct ggttcacgct gccaggatcg gtctccaaaa gattctcgtc gatatcgtag
    28981 agaaattccg acggcacgtt gacctgcttc gagccgaaga tggtgcggat ttgcgcgtgc
    29041 gtgaggaaca ctttcctgcg cgcgcgggta agggccacat agaaaagcct ccgttcctcc
    29101 tcggattcct cggtcggcgc ttcggcttcc gcgcggttcg aggggaacaa gccctgctcg
    29161 aggccggtga tgaatacgta atcgaactcg agccctttcg aagcgtggac cgtcataagc
    29221 ttcgcgccct gccgcgtttc ggagagctcg tcctggtcgg atgcgagcgc cgcgtcggtg
    29281 aggaatttct caaggccttc ttccgggccg aactcgtcgt acttcgccgc gagtgacgcg
    29341 agctccttca cgttttccag ccgctcctgg tcctcctcgc ttccctgcct gtagagcgtt
    29401 tcgaagcccg cttcgcgaag gacgaatcgc acgagctccg acggcctttc atggagcgcc
    29461 gtttcgcgga tgcgcgaaag aaggatatag aacttctcga ctttttcccg catcgcgccg
    29521 gtgaggctct ctcgctggtt ggcgagaacc ttcaagagcg taaccttgcc gatgccgcgg
    29581 gcggggacgt ttatgatgcg cttcaggtcg gagaggctgt cggaattgag cgccgccttt
    29641 atatatgaga gcacgtcctt cacttccttg cgctcgaaga acctgacgcc gagcacctgg
    29701 tatggcacgt cgccgtgaag gaacgcctcc tcgagggcgc gggactggta attcgcgcgg
    29761 tagagcaccg cgatttcgct cggctccgcg ccttcgcgga tgatttgctt ggcggttcgg
    29821 gcgacgaagc tcgcctcgtc ctgctcgtca taggcctcgt agaggcttat tttctcgccg
    29881 tctacgttgt ccgtgaagag atttttctcg ttgcggaatt tgtttttctt gatgatgccg
    29941 ttggccacgg caaggatggt cttggtcgag cggtaattgc gctcgaggag aatgacctgg
    30001 gcgcccggga aatcgcgctc gaagttcagg atattccgga gctgcgcgcc gcgccagctg
    30061 tagatgttct gatcgctgtc gcccaccacg cagatgttcc ggtgcttttc ggagaggagc
    30121 ttcgcgatca tatactgcac tttgttcgtg tcctggta
//
LOCUS       DASZYS010000029         7219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_940166, whole genome shotgun sequence.
ACCESSION   DASZYS010000029 DASZYS010000000
VERSION     DASZYS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940166"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VHF05_01760"
     CDS             complement(<1..1081)
                     /locus_tag="VHF05_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HVS79682.1"
                     /translation="MKTTRQKIAAVLLTAALIALNFPSYTSAYFFDTETSGIATLSAG
                     TLDIEASATSSSMILAPGDDSDFDTSVSDNGSLPFQYGLAPASNSCTASLSSALHISV
                     KEGDADVYSGSIIDAFASTTTLGAATTTLGDWSFKFSLDPSYHSTADESCSLSLAFDA
                     WQTNVPSYGAAGFSDSVAMNFDLSVAANPGVVLNEILPNPEGDDSQHGLQGEWVELYN
                     NGNTPVDLTGWKITDADGHSIPLATTTTWNGRTTIGAKGGGLEWVVVMMNGQILNNDG
                     DTVSLYDADGNLVDSYTYSASVTDGDSDSNNTPDGGNGNPGGSETAGNEGKSYARIPD
                     GTGAWVDPVPTPDMPNELIDPDALLL"
     gene            complement(1078..1632)
                     /locus_tag="VHF05_01765"
     CDS             complement(1078..1632)
                     /locus_tag="VHF05_01765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVS79683.1"
                     /translation="MNTSKFIYNIVFGIIIVIALFMLSTILPLPGHYEIKIVKSGSME
                     PAIHTGSIVVIKPAANYAVGDVITFGKDTKNDVPTTHRIVAERAEEGQMIYQTKGDAN
                     DTPDGETPQTAVIGKVEFSVPFAGYIIAAARQPVGFFFLIIVPAAIVAIEEIGSIWRE
                     IKKMRAKKRNLENSDETRSGETSV"
     gene            complement(1722..2483)
                     /locus_tag="VHF05_01770"
     CDS             complement(1722..2483)
                     /locus_tag="VHF05_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HVS79684.1"
                     /translation="MKKILISVSILAFVGVVVAQTTGAFFTDADTVTGNTFTAGTLNV
                     TLTGNDSTNNTRFDLGNISGMAPGDTTGTAAIDIKNGGSIPLAWFGYFTATSHGADMT
                     QVIYIKSAKMEFLDPSSNEWLPADQFITDGVGSGLYPTEYNLMASSSPYGVVTLAAWN
                     ADNAMGVGPGVQVGALNPNFKYRFSFQLGMVDQAGNTYQGKSMDLSYTVKATQVNSSA
                     ITALGNGDGRIADNLGNVGPGSYWVDWFNTQLSSQ"
     gene            complement(2896..3948)
                     /locus_tag="VHF05_01775"
     CDS             complement(2896..3948)
                     /locus_tag="VHF05_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79685.1"
                     /translation="MKRTIAITASLVAVFAFAISFGAANHAFADEATSSDATSTDTEA
                     TTSPFSVRIMEHVCPDITSVDQFNEMESGLGTKDGFLAAEKACSTTALPGDSPVADAI
                     SGPRATFDYEVTSTISSSSDEASTSTLSISDAAYKTGTLCESDVNEDLNDDGDVATST
                     CLDASYYDFPIDFGENGTTTTTTAPVVTLRETELPDDTSYGGILFTPASIMENTDADT
                     YISSSSTESATSSPVVTLDLSRDSDGDNAVMLHIFHFANASTSTTTGSSTDDNGGDNG
                     NSDNGNGTSTPDLGPIWNALNSRLDDIQSRLSDNFSNLQSRLSDLQDRLSSIFKGDRI
                     VNNGTMNLDDNSSIDR"
     gene            complement(4101..5027)
                     /locus_tag="VHF05_01780"
     CDS             complement(4101..5027)
                     /locus_tag="VHF05_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVS79686.1"
                     /translation="MMYDLAILGGGPAGVAAGVYASRKRLKTVFITTNFEGQSAVSEG
                     VENWIGTIKIPGLELSKNLEAHLRAYAGDVVDIQTGETVQGIEKLGDFHFKVKTDKSE
                     YEAKSVLITTGSVRRKLEVPGAAEFENRGLTYCASCDGPLFAGMDCVVVGGGNAAFES
                     AAQLLAYAKSVTLIHRSETFRADPVTVARVSAHPNMKVVTNVMPTAVKGDKFVTAISY
                     KNNATGEETDLPCSGIFIEIGLIPNTWFLKDVVKLNGNGQIVTDPRTQATSVKGIWAA
                     GDATDGLYHQNNIAAGDAVRALEDIYLELMAK"
     gene            complement(5140..6357)
                     /gene="ftsZ"
                     /locus_tag="VHF05_01785"
     CDS             complement(5140..6357)
                     /gene="ftsZ"
                     /locus_tag="VHF05_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HVS79687.1"
                     /translation="MPQVKPEVEAFARIKVLGAGGSGKNAINHMINSKVKGVEFIVIN
                     TDAQDLHNSLAKKKIHIGKNLTRGLGTGMNPELGKRAVEETKEELQEAIKGADMVFIA
                     CGMGGGTGTGAAPVIARAAKELEALTVAVVTKPFVFEGVQRAKLAEQGLEELKKSVDA
                     IIVIPNDRLLSTIDKNTTMKNAFAMCDDVLKNAVEGISDLITTPGIINIDFADIRAIM
                     ENAGPALMGIGYATGDKRAEEAAKMAINSPLLELSMNGARGVLFSIAGGEDLTMFEIQ
                     DAAKVITESADPQAKIIFGTVKDTKLKKGEVKITVIASGFPEALARKTILGSEAAPEE
                     KQESEKQEDRPRGRIFNTFGGQPAERRERAEQPQREPVARREEEKKPAPPDDDSDDDW
                     GAVPAFLRRSKLK"
     gene            6925..>7219
                     /locus_tag="VHF05_01790"
     CDS             6925..>7219
                     /locus_tag="VHF05_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79688.1"
                     /translation="MIIEYLFDYSVDPQNPEQSLHALTEEIPDGAAVMNKVDLEVGGE
                     IFPFEVNSERHWKWRESRRILRLTVHLIEEDISRKRIRSFRKLLRQDSRWLVVE"
BASE COUNT         1492 a         1864 c         2068 g         1795 t
ORIGIN      
        1 gaaggagaag cgcatcggga tcgataagct cgttaggcat gtccggagtg ggaaccggat
       61 cgacccatgc gcccgtgccg tcgggaatgc gggcgtatga cttgccttcg ttgccggccg
      121 tttccgatcc gcccggattt ccgttgccgc cgtcgggagt gttgttcgaa tcgctgtcgc
      181 catccgtgac gctcgctgag taggtataag aatcaacaag gtttccgtcc gcgtcgtaga
      241 ggcttaccgt gtcgccgtca ttattgagaa tctgcccgtt catcattacc accacccact
      301 cgagcccgcc gcccttcgcc ccgattgtcg tgcggccgtt ccaggtagtt gtggtggcga
      361 ggggaatgct gtgtccgtca gcgtcagtga tcttccatcc ggtgaggtcg acaggagtgt
      421 tgccgttgtt gtagagctca acccattcgc cttggaggcc gtgctgggaa tcgtcgcctt
      481 cgggattggg caggatttcg ttcagcacca cgcccggatt cgccgcgacg gaaagatcga
      541 aattcatcgc gacgctatcg ctaaagccgg ccgcgccgta tgaagggacg ttcgtctgcc
      601 atgcgtcgaa tgcgaggctc agcgagcatg attcgtctgc cgtggaatga taggagggat
      661 cgagagaaaa tttgaacgac cagtcgccga gcgtcgttgt tgcggctccg agcgtcgtcg
      721 tcgaagcgaa tgcgtcgatg atgcttcccg agtacacatc cgcgtcgcct tccttcacgc
      781 tgatgtggag cgccgacgat agggaagcag tgcaggaatt cgaggcggga gcgaggccgt
      841 attggaacgg caggcttccg ttgtcggaaa ccgatgtgtc aaaatcggaa tcgtcgcctg
      901 gagcgaggat catcgaggac gatgtggcag atgcctcgat atcgagtgtg ccagccgaaa
      961 gcgtggcgat gcctgacgtt tcagtatcga agaaataggc tgaggtatac gaaggaaaat
     1021 tcaacgcgat aagcgccgct gtgagaagaa ccgccgcgat tttttgcctc gtagttttca
     1081 tacgcttgtt tcgccgcttc gggtttcgtc gctgttctca agattccttt tcttcgcccg
     1141 cattttcttg atttcgcgcc aaatcgatcc gatctcctcg atcgcgacga ttgccgccgg
     1201 cacgatgatg aggaagaaga agccgaccgg ctggcgcgcc gcggcgatga tgtagccggc
     1261 gaagggaact gagaattcca cttttccaat gactgccgtt tgaggcgtct ctccgtcggg
     1321 cgtatcgttc gcatcgcctt tggtctggta tatcatctgc ccctcctcgg cccgctccgc
     1381 gacgatgcgg tgcgtggtag gcacgtcatt tttcgtatcc ttgccgaagg tgatgacgtc
     1441 gcccacggcg tagtttgcgg caggcttgat gaccacgatg cttccggtgt ggatcgccgg
     1501 ctccatggag cccgatttca cgatcttgat ctcatagtgt cccggcaacg gcaaaattgt
     1561 cgacagcatg aaaagggcga tcacgatgat gatgccgaag acgatgttgt agatgaattt
     1621 cgacgtgttc atgtcattgc tgggctttaa agcctttatc ctcccgccct cctaagagag
     1681 cgagagggaa aagctataag ctaattcaac ttgaaaaagt cttattgact tgaaagctgt
     1741 gtattgaacc agtctaccca atacgatccc ggtccaacgt tgcccaagtt gtcagcaatg
     1801 cgtccatccc catttccgag agccgtaatt gctgatgaat tgacctgagt tgccttcact
     1861 gtgtagctca aatccataga cttgccctga taggtatttc ctgcctgatc taccattcca
     1921 agctggaagc tgaacctgta cttaaagttc ggattgagtg caccaacctg tacacctgga
     1981 cctacgccca tagcattgtc tgcattccat gctgcaagcg tgactacgcc gtaagggctg
     2041 ctgcttgcca tgaggttgta ctcagtaggg taaagtccgg agccaactcc gtctgtaata
     2101 aactgatctg caggaagcca ttcgttactg ctcggatcga gaaattccat ctttgccgac
     2161 ttgatgtaga taacctgcgt catgtcagcg ccatgacttg tggcggtgaa ataaccaaac
     2221 catgcaagag gaatacttcc tccgttttta atgtctatcg ctgctgttcc tgtcgtgtcg
     2281 ccaggagcca tgccagaaat atttcccaga tcaaagcggg tgttgttggt gctgtcattt
     2341 ccggtgaggg tcacattaag agttcctgcc gtgaaggtgt ttcccgttac agtgtcagca
     2401 tccgtgaaga atgcgccggt cgtctgtgcg acgacgactc ctacgaatgc aagaatactg
     2461 acgcttataa gtatcttttt cattattaat tgttatgact gaataaaaac cgaacgagtt
     2521 ttttctcgta tcggcaactg acattttcaa actccagatc ggcgcggcgc gcaatcgacc
     2581 caatgaaagc accacagaac gcgtttcgaa tcgcttcgaa ccgcctcgtg tgccgtcttg
     2641 attggcacgg ggagcctctc taccatcttc caggctagct caacggtagc caaaataaaa
     2701 gtgcagatta gtacggcaac ccgtcggcag gcaatgtcaa catgatagtt tttgtccgtc
     2761 aaaacatgtt gaaaaacctg gcctttttag ctgtctttta gctcgtgtcc tttagaatat
     2821 gcgatccatt caaaatcaaa tatgggaaaa acaaaaaccg ccccctggcg agggcggttt
     2881 ttcagacgat agaatttacc tatctatcga cgagttgtcg tcgagattca tagtcccgtt
     2941 gtttacgatg cggtcgcctt tgaatatcga gctcaggcga tcctgcaggt cgctcagcct
     3001 gctttgcaga tttgagaagt tgtcgctcaa gcggctctgg atatcatcca ggcggctgtt
     3061 taaggcgttc cagatggggc cgagatcagg ggtgctcgtt ccgtttccat tgtcggaatt
     3121 gccgttatcg ccgccattgt cgtcggtcga gcttccggtc gtggtgctcg tcgatgcatt
     3181 cgcaaagtgg aagatgtgga gcattactgc gttgtcgccg tcgctgtcgc gcgaaagatc
     3241 gagggtgacg acggggctac tcgtggcgct ttcggtcgag ctgcttgaaa tgtatgtatc
     3301 ggcatcggta ttctccatga tcgaagcagg cgtgaagagt attcctccgt agctcgtgtc
     3361 atcgggcagt tccgtttcgc gcagggttac caccggagcg gtcgtggtgg tcgtcgtgcc
     3421 gttctcgccg aagtcgatcg gaaaatcata gtatgaggca tcgaggcagg tcgaggtggc
     3481 gacgtcgccg tcatcgttca aatcctcgtt aacatccgat tcgcagagcg tgccggtctt
     3541 gtaggctgcg tccgagatgc tcaaggtgct ggtcgaagct tcatcggaag acgaagatat
     3601 ggtgctcgtc acttcataat cgaacgtagc gcgcggtccc gagatcgcat ccgcgaccgg
     3661 cgagtcgccc ggaagcgccg tagtcgagca ggctttttca gcagctagga atccgtcctt
     3721 ggtccctaag ccgctctcca tttcattgaa ttggtcgacg ctcgtgatat cagggcatac
     3781 gtgctccatg atccgaacgg aaaacgggct ggtggtggcc tcagtatcgg tcgatgtcgc
     3841 gtcggaagat gtcgcctcgt ccgcaaacgc gtgattggct gccccgaagc ttatcgcgaa
     3901 tgcgaatact gctacaagag acgccgtaat ggcaatagtt cttttcataa aaattcatgc
     3961 aaaggttact actaatactt tgcgtgagca ttttaggaaa ctctcatgaa ccggttgtga
     4021 aaaaactccg aaactctcga gtagaatctc aagctgggca ttgaggatgg cgaaatgaaa
     4081 aaacgcccga aggcgttttt tcatttcgcc atgagttcga gatagatatc ctcgagagcc
     4141 cgcactgcgt cgcctgcggc gatattattt tgatggtaga ggccgtcagt tgcgtcgccc
     4201 gccgcccaga tgcctttgac gctcgttgcc tgagtgcgcg gatcggtgac gatctggccg
     4261 tttccgttga gcttgacgac atctttcagg aaccacgtgt tcggaatgag gccgatctcg
     4321 atgaaaatgc cggagcaggg gagatccgtc tcttcgccag tggcgttgtt cttgtaggaa
     4381 atggccgtga cgaacttgtc gcctttgaca gcggtcggca tcacattggt cacgactttc
     4441 atgttcgggt gcgcggaaac gcgggcgacc gtcactgggt cggcgcggaa ggtttcgctc
     4501 ctatggatga gcgtgacgct tttggcgtat gcgagaagct gggcggcgct ttcgaatgcc
     4561 gcattgccgc cgccgacgac aacgcaatcc atcccggcga agagcggtcc gtcgcaggag
     4621 gcgcagtagg tgaggccgcg gttctcgaat tccgccgcgc cgggcacttc gagcttgcgg
     4681 cgcaccgagc cggtggtgat aagtacgctc ttcgcttcat actcgctttt atcggtcttt
     4741 accttgaaat ggaagtcgcc aagcttctcg atgccctgga ctgtttcgcc ggtctggata
     4801 tcgacgacat ctcctgcata ggcgcgcaga tgcgcctcga gattcttgga aagctcgagg
     4861 ccggggattt tgatcgtccc gatccagttc tcaacacctt cagagacggc gctttggcct
     4921 tcgaaatttg tcgtgataaa cacggtcttc aagcgctttc ttgaggcata aactcctgcg
     4981 gcgacgcctg ccggcccgcc gcccaaaatt gcgagatcgt acatcatgaa agaagtatac
     5041 ccgagacaat agacactaga caatagactg actgaaaccg cctggcgcgc atgcgcgcca
     5101 ggcggtgttt tcgttatgag ttaatttgag tctccgctgt tacttcagct tggatcggcg
     5161 gaggaatgcc ggaactgcgc cccaatcgtc gtccgaatcg tcgtcaggcg gagcgggctt
     5221 cttctcttcc tcgcggcggg cgacaggctc gcgctggggc tgttccgcgc gctcgcggcg
     5281 ctccgcgggc tggccgccga aggtgttgaa gattcggccg cgagggcggt cttcctgctt
     5341 ctcggactcc tgcttctctt cgggcgccgc ttccgagccc aaaatggtct tgcgggcgag
     5401 cgcctcgggg aagccggagg cgatcacggt gatcttcact tcgcctttct tgagcttggt
     5461 atccttgacg gttccgaaaa tgatcttggc ctgcggatcg gccgactcgg tgatgacttt
     5521 ggctgcgtct tggatctcga acatcgtaag gtcttcgccg ccggcgatcg agaagaggac
     5581 gccgcgggcg ccgttcatcg agagctcgag gagcggggaa ttgatcgcca tctttgccgc
     5641 ctcttcggcg cgcttgtcgc cggtggcgta gccgatgccc atgagcgcgg gccccgcgtt
     5701 ctccatgatg gcgcggatgt cggcgaagtc gatgttgatg attccgggcg tggtgatgag
     5761 gtcggaaatg ccctcgaccg cgttcttaag cacgtcgtcg cacatggcga aggcgttctt
     5821 catcgtggtg ttcttgtcga tggtggagag gaggcggtcg ttcgggatga cgatgatcgc
     5881 gtcgaccgac ttcttgagct cctcgaggcc ctgctcggcg agcttggcgc gctggacgcc
     5941 ttcgaagacg aagggcttgg tgacgaccgc gacggtgaga gcttcgagct ctttcgccgc
     6001 gcgggcgatg acaggagccg cgccggtacc ggtgccgccg cccatgccgc atgcgataaa
     6061 caccatgtcg gcgcccttga tcgcttcctg gagctcctct ttggtctctt cgaccgcgcg
     6121 cttgccgagc tccggattca tgccggttcc gaggccgcgg gtgaggttct ttccgatgtg
     6181 gatcttcttc ttcgcgagcg agttgtggag gtcctgcgcg tcggtattga tgacgatgaa
     6241 ttcaacgccc ttcaccttgg agttgatcat gtggttgatg gcgttcttgc ccgagccgcc
     6301 cgcgccgagg actttgatgc gcgcgaaggc ttctacttcc ggtttgactt gtggcatggt
     6361 ggtaatggtg gggaaaggct gctaattact caataaaatt cccgttgggt atcatcataa
     6421 cagaaggact ttaaaatata aatatcttgc taattcttaa atttgtgtcg aaaaatcata
     6481 gctttttccc atatttaatc gtatgatagc gaacggatga gttttttgag gaggcaattt
     6541 ggtattcaaa gtccatataa acaatacgcg gtcgcgtagg tggaaaacgc agcgcgaggc
     6601 ggatcaggcg attttctcat taggcaggat agcactgccc gtaaataaca ggggatatga
     6661 gtttttggtg cagccgcctc gggacggtat aactccgatt cttccgagcg tccgggagcc
     6721 tttgaatgaa agagagctca tacgggatct cgacgaagat ccgaattggg tagaggcgga
     6781 ttaagcccga atatccgcag tgccttttag cggcgctgcg gatttcttta atagttatat
     6841 aactcgtacc cctccaattt tctttcgtta gtatatcctt gaatttttta attattatgg
     6901 tattataaaa acgaaaggag gttcatgatc atcgagtatc tttttgatta cagtgttgat
     6961 cctcaaaatc ccgaacagtc gctccatgct ctgacggaag agattccaga tggtgcggcg
     7021 gttatgaata aagtcgatct cgaagttgga ggagaaatct tccccttcga agtcaattcc
     7081 gaacggcatt ggaaatggag agaatcacga cgcatcctgc gactcaccgt gcatcttatc
     7141 gaggaggata tctctcgcaa gcggatcaga tcgtttcgaa aactgcttcg gcaggacagc
     7201 cgttggctgg ttgtcgaag
//
LOCUS       DASZYS010000030         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_864395, whole genome shotgun sequence.
ACCESSION   DASZYS010000030 DASZYS010000000
VERSION     DASZYS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864395"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2381
                     /locus_tag="VHF05_01795"
     CDS             102..2381
                     /locus_tag="VHF05_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HVS79689.1"
                     /translation="MHSFEIEKTGDEKNGDSLLDIKILPNRAHDCLSHRGIAGEVAVL
                     FDLKMKGERFEKFGNDSGEAKIAVNVEDSKLCPRYVGRLVENVWVKESPAWLKERLES
                     VGQRSINNIVDITNYVMLDTGQPMHAFDADKVRGGISVRKAKVGERIETLDGKDVELD
                     ESILVIADDEGPLAIAGVKGGKRAEVDAGTRRVILEAANFNPGNVRKTSQKINIKTDA
                     SKRFENEITPELAGEGMEYATALIVQEVGRDAVVHAPADEYPRKRQPYKLGVSLSEIN
                     RLLGTPISEETVAAILDRFGWEWEVVEPREYILELAPQCVGAPYKLGASVSIDAPREF
                     DCSSFTAFVYSQAGISIPRMSIDQYVYGEPVDDPKDLKPGDLLFSNSGNGKVHFESIE
                     WMKGAEVPGGIDHVGIFLGDDKVIHASRNGESSVQIERASESKFFKEFIGARRISGLD
                     EKRFVVTVPAARLDLRLKEDLIEEIGRVYGYEKIAARLPESRGEAKVNKAFYYAQTIR
                     NILTGLGFDEVIDYAFRNEGEVEVENPLASDKKFLRSDLSSGLGEALEFNARYADLLG
                     LDEIRIFEIGNVFAGDTEDLRLSIGVKNVKKDKKKEDEKAGEALIKLFEKLGCAPESE
                     TEMKNGIAEISLSKLVGQLPEVKEYGDILATHKKNIRYKSLSPYPFVLRDIAVWMPAE
                     TPAHELEAVLLEMGGPLLAAHRLFDTFTKEFDTGEKKTSYAFRLVFQSMEKTLSDDEV
                     NGIMSRVNDKVMSKGWEVR"
     gene            2418..2786
                     /locus_tag="VHF05_01800"
     CDS             2418..2786
                     /locus_tag="VHF05_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVS79690.1"
                     /translation="MMQAKSYKNLIVWQKSMQLVKEIYKLTDQMPHREVYGLADQMRR
                     AAISVPSNIAEGSQRATRKDFSQFLRIARGSAAELETQLLLTKDLYPKINLQTAHELL
                     DEIGRMLLALSRKLISPSKN"
     gene            2793..3317
                     /locus_tag="VHF05_01805"
     CDS             2793..3317
                     /locus_tag="VHF05_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HVS79691.1"
                     /translation="MTNKLLTVPFYSQYIDIADPHWIIRSCGITCVKMALDYFGAKTP
                     ALDDLIKEAVAMGAYGDSGWYHDGLIAVAEKYGAEAYRKEDMDNKDGVLELFNALENG
                     SLVIVSVVQRLLGRKKYHQVLLVGYRESEHGLQGFYYHDPASTDREQGKGLYVNLDVF
                     KVYWRNKAIFLKSK"
     gene            complement(3364..3681)
                     /locus_tag="VHF05_01810"
     CDS             complement(3364..3681)
                     /locus_tag="VHF05_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79692.1"
                     /translation="MIGYTENTRSVDNIVTALFVVEDSPIRIIDRWGLNQQIDDHSPI
                     YWIDFDGNEHITTPAEFKNFVSKYGLAVQTCLTANYGRDPENRIILTDASINLLYVIK
                     NET"
     gene            3882..>5631
                     /locus_tag="VHF05_01815"
     CDS             3882..>5631
                     /locus_tag="VHF05_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HVS79693.1"
                     /translation="MAKEKVKKSEYGADSITVLEGLEPVRKRPGMYIGTTGPEGLHHL
                     IWEIFDNSRDEAMNGHADHIEVVLLPGEKNPDGTPGERVRVVDNGRGIPVGVHKVTKV
                     SALETIMTTLHAGGKFGGEGYQVSGGLHGVGASVVNALSTYAQVEVHQDGAKYMQEYA
                     RGKKKAAVKKVGTTKARGTVVTFQPDPEIFKEGTSFDWEKIVERLKQQAYLVKGLRID
                     IIDLRGYAGKIDLDKAFYLKPMGYEAPSMTFYFEGGLVSFVRSNNAAQKPIHKNVFYV
                     EKANDNGESVEIALQYVDDISPRIMPFANNIYTPEGGTHVTGFRTALTRTLNTYARKN
                     NLVKEEDSFTGEDVQEGLAAVISVKLREIQFEGQTKAKLGSMEAQGMVATVFGDAFAT
                     FLEENPDDGKAIVQKVVLAVKARKAAKAAKDSILRKGALEGMTLPGKLADCQSGTSAE
                     DAELFIVEGDSAGGTAKTGRDRKTQAILPLRGKILNIERARLDRMLASEQIKNLVIAL
                     GTAIGDTFDITKLRYHKIIIATDADVDGAHIRTLILTLLYRYFKPLLDLGHVYIAQPP
                     LYKIKKGKEIFYAYSDV"
BASE COUNT         1413 a         1506 c         1542 g         1170 t
ORIGIN      
        1 cgttgtaaat cagttctaac atgttcatct cacgcaactg gctaaaaaca ttctttaata
       61 ctgatattcc atctgtaaaa aaaatagcag aagctattac catgcattcg tttgagattg
      121 aaaaaactgg agatgaaaaa aatggtgact cgcttctcga catcaagatc ctgccgaatc
      181 gcgcgcacga ctgcctctcg caccgcggaa tcgcgggcga ggtcgcggtg ctgttcgatc
      241 tcaagatgaa aggcgagcgg ttcgagaaat tcggaaatga ttccggcgaa gcgaaaatcg
      301 ccgtgaatgt cgaggattcg aagctctgcc cgcgctatgt cggccgcctt gtcgaaaacg
      361 tatgggtgaa ggaatcgccc gcctggctca aagagcgact ggaatctgtg ggccagcgct
      421 cgatcaacaa catcgtcgat attacgaatt atgtgatgct cgatacgggc cagccgatgc
      481 atgcgttcga tgccgacaag gtgaggggcg gcatttcggt gcgaaaggcg aaagtgggag
      541 aaaggattga aacgctcgac ggcaaggacg tcgagctcga tgaatcgatt ctcgtcatcg
      601 ccgatgacga agggccgctt gcgatcgctg gcgtgaaggg cggcaagcgc gccgaagtcg
      661 acgcgggaac gcggcgcgtc attttggaag ccgcgaattt taatccgggg aacgtgcgca
      721 agacctcgca gaaaataaat ataaaaaccg acgcttcgaa gcgcttcgag aacgaaataa
      781 cgcccgaact cgcgggagag ggaatggaat acgcgaccgc gctcatcgtg caagaggtcg
      841 gccgcgacgc ggtcgtccac gcgccggccg acgagtatcc gcggaagcgc cagccctaca
      901 agctcggcgt gtcgctttcc gaaatcaacc gtcttctcgg cacgcccatt tccgaggaaa
      961 cggttgccgc tattctcgat cgcttcggat gggagtggga agtcgtcgag ccgcgggaat
     1021 atattttgga actcgcgccg caatgcgtcg gcgcgccgta caagcttggc gcgagcgttt
     1081 ctatcgacgc gccgcgcgaa ttcgactgct cgtcgttcac ggcattcgtt tactcgcagg
     1141 ctggaatctc gattccacgc atgtcgatcg accagtatgt ctatggcgag ccggtcgacg
     1201 acccgaagga cctcaagccc ggcgacctgc tcttttcgaa cagcggcaac ggaaaagttc
     1261 atttcgaatc gattgaatgg atgaagggcg ccgaagtgcc cggcggcatc gatcacgtcg
     1321 ggatctttct cggcgatgac aaggtcattc acgcttcgag aaacggcgaa agcagcgtgc
     1381 aaatcgagcg cgcatcggag agcaagtttt tcaaggaatt cattggcgcg cggcgaatct
     1441 cgggcctcga cgagaagcgc ttcgtcgtga ctgtgccggc ggcgcgcctc gaccttcggc
     1501 tcaaagaaga cttaatagag gaaatcggcc gcgtgtacgg ctatgagaag atcgcggcgc
     1561 gcctgcccga aagtcgggga gaagcgaagg tgaacaaagc tttctattat gcgcaaacga
     1621 tacggaatat cctgaccggt ctcggcttcg acgaagttat tgactatgcg ttccgcaacg
     1681 agggcgaggt cgaagtcgaa aaccctctcg cgagcgacaa gaaatttttg cgaagcgacc
     1741 tttcgtcagg tttgggtgaa gcgctcgagt tcaacgcgcg ctatgccgac cttctcggcc
     1801 tcgatgagat caggattttt gagatcggga acgtatttgc cggcgatacc gaagacttgc
     1861 gcctctcgat cggcgtgaag aacgtgaaaa aagacaagaa gaaggaagac gagaaggcgg
     1921 gggaagcgct cataaagctt ttcgagaagc ttggctgcgc gcccgagtcg gaaacggaaa
     1981 tgaaaaacgg cattgcggaa atctctctct caaaattggt cggccagctt cccgaggtga
     2041 aagaatacgg tgacattctc gctacgcaca aaaaaaacat ccgttacaaa tccctctccc
     2101 cgtatccgtt cgtccttcgc gatattgccg tgtggatgcc cgccgaaact cctgcacacg
     2161 aacttgaagc ggttttgctt gaaatgggcg ggccgcttct cgcggcgcac aggcttttcg
     2221 atactttcac gaaggagttc gataccgggg agaaaaagac ttcctacgct ttccgcctcg
     2281 tcttccagtc gatggaaaag acgctttccg acgatgaggt gaacggcata atgagccgcg
     2341 tgaatgacaa ggttatgtcg aagggatggg aagtgagata agaagttgtt agtttatagc
     2401 tgctagtttc tagaaaaatg atgcaagcaa aatcctataa aaacctgatt gtgtggcaaa
     2461 aatccatgca attggtgaag gagatttata aattaacgga tcaaatgccg catcgcgagg
     2521 tatatggctt ggctgaccaa atgagaaggg cggcaatcag tgttccttcc aatatcgctg
     2581 aaggaagtca aagagcgact cgaaaagatt tttcccagtt cttaagaatc gctcgaggtt
     2641 ctgcggctga acttgaaact caacttctgc tgactaaaga tctgtatccc aaaattaatc
     2701 tccaaacggc acacgaactt cttgatgaaa tcggaagaat gctgcttgca ttgagtcgaa
     2761 aactcatttc cccttctaaa aactagaatc taatgactaa caagcttctt accgttccgt
     2821 tttattccca atacatagat attgcggatc cgcactggat tattcgcagc tgcggcatca
     2881 cctgcgtcaa aatggcgctc gattatttcg gggcgaagac gcccgctctc gacgatctca
     2941 tcaaggaggc ggtcgccatg ggcgcctacg gagattccgg ctggtaccat gacgggctta
     3001 tcgccgtagc cgagaaatac ggcgcggagg cgtaccgcaa ggaagacatg gacaacaagg
     3061 acggcgtact cgaattgttc aatgcgcttg agaacggcag ccttgtcatc gtttcggtcg
     3121 ttcagcgcct tctaggccga aaaaagtatc accaggtgct tctcgtaggc taccgcgaga
     3181 gcgagcacgg tctccagggc ttttattatc acgatccggc gtcgaccgac cgcgagcagg
     3241 ggaaagggct atatgtaaat ttagacgtct ttaaggttta ttggagaaat aaggctatct
     3301 ttttgaagag taaataatgg ttagtagata gaaaaaggcc gtgcgacaga gtcgcacggc
     3361 gcttcatgtc tcatttttta taacatacag cagattgatg cttgcatcag tgagaatgat
     3421 ccggttctcg ggatcacggc cataatttgc cgtaaggcaa gtttggaccg cgaggccgta
     3481 tttggaaacg aaatttttga actccgcagg cgttgtgatg tgctcgttcc catcgaaatc
     3541 gatccagtaa atcggcgaat gatcatcgat ctgctggttc agcccccacc tgtcgattat
     3601 gcggataggg gaatcttcca ctacaaaaag cgctgtgacg atattgtcga cggacctcgt
     3661 attctcggtg tatccaatca tgtatcctcc tggaataaac taatcatttt ttaaggccat
     3721 gtgtcaactt ttttgaccac gaattctgct tggtttcggc ctcaaaattt gctataattt
     3781 cactacgaaa agagcattcc gcttggtttt tcttttgctt ttttctccct ttgtatcctc
     3841 tagaaactag caactataaa ctaaaaactt cccccatccc aatggccaaa gaaaaagtaa
     3901 agaagtccga atacggagcc gattcgatca cggtcctcga gggtcttgaa ccggtgcgca
     3961 agcgcccagg catgtatatc ggcacgaccg gtccggaagg cctccaccac ctcatctggg
     4021 aaatcttcga caactcgcgc gacgaggcga tgaacggcca tgccgatcac atcgaagtcg
     4081 tccttttgcc cggcgagaag aatcccgacg gcactcccgg cgagcgcgtc cgcgtcgtcg
     4141 acaacggccg cggcattccg gtcggcgtcc ataaggtgac gaaagtctcc gcgctcgaga
     4201 cgatcatgac caccctccac gccggaggca agttcggcgg cgaaggctac caggtttccg
     4261 gcggcctcca cggcgtcggc gcgtccgtcg tgaacgccct ttccacatac gcgcaagtcg
     4321 aggttcatca ggacggcgcc aagtacatgc aggaatatgc gcgcggcaag aagaaggcgg
     4381 cagtgaagaa agtcggcacc acgaaagccc gcggcaccgt cgtaacgttc cagcccgatc
     4441 cggaaatttt caaggaaggc acgtcgttcg attgggagaa gatcgtggag aggctcaaac
     4501 agcaggcata cctcgtgaaa ggcctccgca tcgacatcat cgatctccgc ggctacgcgg
     4561 gaaagatcga tctcgacaag gcgttctacc tcaagcccat gggctacgaa gcgccgtcga
     4621 tgaccttcta tttcgaaggc ggcctcgtat cgttcgttcg ctccaacaac gccgcgcaga
     4681 agccgatcca caagaacgtg ttctacgtcg agaaggcgaa tgacaacggc gaatcggtgg
     4741 agatcgcgct ccagtacgtc gacgacatct cgccgcgcat catgccgttc gcgaacaaca
     4801 tctatactcc ggaaggcggc acgcacgtta ccggcttccg caccgccctc acccgtacgc
     4861 tcaacaccta cgcacgcaag aacaacctcg tgaaggagga agacagcttt accggcgagg
     4921 acgtccagga aggtctcgcg gcggtcattt ccgtgaagct ccgcgaaatc caattcgaag
     4981 gacagaccaa ggcgaagctc ggctcgatgg aagcgcaggg catggtcgcc accgtcttcg
     5041 gcgacgcatt cgcgacgttc ctcgaggaaa atcccgacga cggcaaggcc atcgtccaga
     5101 aagtcgtgct cgcagtgaag gcccgcaagg ccgcgaaggc cgcgaaggat tcgatcctcc
     5161 gcaaaggtgc gctcgaaggc atgacccttc cgggcaagct cgccgactgc cagagcggca
     5221 cgagcgccga agacgccgag cttttcatcg ttgagggaga ttccgcaggc ggcaccgcaa
     5281 agaccggccg cgataggaag acgcaggcaa tcctgccgct ccgcggaaag attctgaata
     5341 tcgagcgcgc ccgcctcgac cgcatgctcg catccgagca gatcaagaac ctcgtcatcg
     5401 cgctcggcac cgcgatcggc gacactttcg acatcacgaa gctccgctac cacaagatta
     5461 tcatcgcgac cgatgccgac gtcgacggcg cgcatatcag gacgctcatt ctcacgttgc
     5521 tctaccgtta cttcaaaccg cttctcgact tgggccacgt ctatatcgcc cagccgcctc
     5581 tctataagat caagaaggga aaggagatct tctacgcgta cagcgatgta g
//
LOCUS       DASZYS010000031        11925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_357968, whole genome shotgun sequence.
ACCESSION   DASZYS010000031 DASZYS010000000
VERSION     DASZYS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357968"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VHF05_01820"
     CDS             complement(<1..827)
                     /locus_tag="VHF05_01820"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVS79694.1"
                     /translation="MENNLEHKRHSLAHLLAAAVLELYPGTKNTLGPAVDNGFYYDFE
                     FASPVADEDLAKIEAKMKELLPSWQTFQKIDAAADDAKKIFADNPYKLELIDEIASKG
                     EPLTLYYSGPRDSSPSLSDLLKTSNSKLATGFFDLCRGGHADNPAKDIAKDSWKLSRI
                     AGAYWRGDEKNKMLTRIYGLAFDTKDELEAYEKMVEEAKARDHKKLGRELDLFVFSDL
                     VGAGLPLWTPKGTELRNLLDDFVWSLRKSRGYMKVEIPHITKKDLYMTSGHWDKFKD"
     gene            1041..1784
                     /locus_tag="VHF05_01825"
     CDS             1041..1784
                     /locus_tag="VHF05_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79695.1"
                     /translation="MKTIATTMKYAALVGIFALAGFATASTVNAAGELSVTCAPNYSS
                     VVEGDGMTFTAYPSGGTELYNYSWSGDDGLGGGIQSINAVYTSTGTKDAHVVVTSGSK
                     TGEADCSVDVIPGQTSSNNTQTNTNTNGNGSIFGTTDNGSNGNPNVTVYTNTSGQVAA
                     NYNGSQYNGGQTGQSGVYLSQVPYTGAMDNFHLALYAISFFLWSGLASYWILKKKYGK
                     ELALAAAGEKNDEDELAARIVSLRTAAAR"
     gene            complement(1857..2168)
                     /locus_tag="VHF05_01830"
     CDS             complement(1857..2168)
                     /locus_tag="VHF05_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79696.1"
                     /translation="MPSIMILARNGSGMVLHEPTDDEFRLYRYVVRMLSDSTTLKQAE
                     ELAARNWHRLKEIADDFKTKRPEVVLSRLTEEPVQEGFAKNPPARRGLKTFLKRLLPA
                     C"
     gene            complement(2316..5507)
                     /gene="dnaE"
                     /locus_tag="VHF05_01835"
     CDS             complement(2316..5507)
                     /gene="dnaE"
                     /locus_tag="VHF05_01835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVS79697.1"
                     /translation="MTKFVHLHTHSHYSLLNALPQIPDLVAAAKKAGFESLALTDNGN
                     LYGAIEFYEKCRKEGIKPIIGVDAYVAARTRFDKEARLDNARSRLVLLAKDGSGYKSL
                     LQMVTDSFLEGFYYKPRIDRELIEKYREGLICINPSFSGDIALAVKSDNLEKARELAE
                     WYKSVFGEDFYIEITHHPEIQGHEEAMAKLVEFAKNAGVPIMAAHDVYYLKKEDARAR
                     EVLMSIANGGGPRSDALSEFESQEDFSFIDGKTAEKYFKKLPEALENTAKIAELCNCE
                     IALGEWKFPNIEIPAGRTADEVLRDIVFEGIEKRGLEKTQEVLDRIEYELKVIKDKGY
                     AKYFLVVSDLLRHAHENGILTTIRGSVGGSLVTFLAFITNVNPLVYKLPFERFLNPER
                     PSAPDIDMDYADNRRDEMISYAKQKYGADKVAQIGTFGTMMARGAVRDVARALGFPYG
                     TGDTIAKLIPMGSQGFPMTIDRALKMEPDLKRMYQSDPDAKEIIDMAKKIEGCARHIS
                     VHAAGVVISPTPLTDYVPLQLDPKGEGKVITQYDMHSVDENAAGLLKFDFLGIRNLSI
                     LADAVSLVKKIEGIDIDIENIPLDDKKTFEMLARGETIGLFQLNGTGMTRYLMDLKPT
                     TIFDINAMVALYRPGPMEMIPEYVKRKHDPSLVKYLDPRLKDILDQSYGIITYQDDVM
                     LIAIHLAGYSWLEADKLRKAMGKKIPAEMEAQKTKLHEGFLANGLSEEKAKTLWSLIE
                     PFAAYGFNKAHAASYGKVAYQTAYMKANFPAIYMSAVLTAESGDTDTVAEVINECTRM
                     NIPVLPPDINESFEGFTVVKTNGEGDKIRFGLTTIKNFGEGIAHAIIEERKRGGKFAS
                     LADFLDRVTDRNLNKKSLESLVKCGAMDAFGERGQMLANLESMVRYNKEGSTTAAGQT
                     SLFGLMSDTSTIPTLKLDSAPPASQSDKLSWEKELLGLYISGHPLDRFKEKFAARGEN
                     IKLIKETKKEGQEVVVGGIIAECKDIYTKKGDKMLFLTVSDLSDSVEMVVFPRVYAEF
                     HDLLKPENCVVIKGKISHRNGSVSMIADKVRLL"
     gene            5665..6306
                     /locus_tag="VHF05_01840"
     CDS             5665..6306
                     /locus_tag="VHF05_01840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVS79698.1"
                     /translation="MQKPVKRVKKAEFVVGIDEAGRGPLAGPVAVGSVLAGAAELSRL
                     RRVFKSIKGKDSKKLTEAEREEWFAVIKAEADAGNLTWRVSLSGSAMIDKKGIVPAIR
                     SALSRSLVAVADDSSRTRVLLDGGLKAPAEFSNQKTIIKGDEKELLISLASICAKVTR
                     DRYMCRLAKKYPEYGFEVHKGYGTKRHAEGIKSFGLCALHRKSFIRKNFNFLK"
     gene            complement(6702..7580)
                     /locus_tag="VHF05_01845"
     CDS             complement(6702..7580)
                     /locus_tag="VHF05_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79699.1"
                     /translation="MTRKTIPSSDPLSIIKSIVDSDIDADRIDFVRRDGQLAGGEYGH
                     YDIRRLCDSAFIERDENGWFLAYSEKALTSMEALLYDRYRTHVWIHFHHSVIAMKMLV
                     RFLIQKAFEQGMITKESFSLNNIHSFALRDDIWLWNILRGLRQENNTTFDMIKRAVFY
                     REKENTLNLWKMRTDYHEIEDAVKSRARVNAVDYGIFDFYIHNLEVNTGVKVFSFPVK
                     FTSISKREVPLYSESSKKLTGKSLLKASKIVSDIETMMKDEPQQFFMLVGENARAHAS
                     KLRQDWIDLTARWVNR"
     gene            complement(7577..8122)
                     /locus_tag="VHF05_01850"
     CDS             complement(7577..8122)
                     /locus_tag="VHF05_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVS79700.1"
                     /translation="MLEFTLRVRDLVHGTIYFTDLERRVINHPFFQRLRHVRQNDVAF
                     YVYPSVNTSRFEHVLGTSHVASKMAEHLGRSPLRDEYLDGLKKQAGIESQDEFVQLVS
                     LYALLHDIGHLPLSHLFEMAATEYWISERQKRMEAIKEWTNTEGFEKLHEAFGAKIIN
                     ELASDPAIGMSGQTKMHSFSL"
     gene            8415..8933
                     /locus_tag="VHF05_01855"
     CDS             8415..8933
                     /locus_tag="VHF05_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79701.1"
                     /translation="MPVLTQEFTKLEDWARAHDPSQTNLLFKNGFWDQIEFVRDQIPG
                     IAYVACGLEAARYVETNIRVISTHVSKSVTLPVFHLRLPNGDQFVMRYNFHDWKVSVA
                     AKEPIDADFMRLFNPSLKLHRAYCEGFPEEWIFGSYEADPGKFSVVLQSGYYYIFTFF
                     WIYFQKVVAKRI"
     gene            9087..9308
                     /gene="rpmF"
                     /locus_tag="VHF05_01860"
     CDS             9087..9308
                     /gene="rpmF"
                     /locus_tag="VHF05_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HVS79702.1"
                     /translation="MRHTRAHRDNRRAHHALEGSALTVCPNCGAKMQRHTACQNCGQY
                     KGRAVVDVHAELRKKQSKKNASSETAAEK"
     gene            9419..10252
                     /gene="nusB"
                     /locus_tag="VHF05_01865"
     CDS             9419..10252
                     /gene="nusB"
                     /locus_tag="VHF05_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVS79703.1"
                     /translation="MQDGDAMDIFHRNAEEFAPGMGDVSFMETLLKNTLEKRKDLDLI
                     IEKAAPDWPLDKISIIDRNILRIGLYELLFSDRAQVPPKVAINEAIELAKSFGGETSG
                     RFVNGVLGAVYKEMGEPGKDEVSKRKDNPGKPVPVERLAGAVIFGVKDGEIHLALVHD
                     IFGHWTLSKGHLTEGEEEEAGVKRVVKEELGIDIAPKSKLGENEYGTYDPEKGKIKKH
                     VSYFLAECDVCELELAESGGLDNAKWFPLADIIDLNFYNDIYPIVTKAVNLLLKENAA
                     A"
     gene            10400..11092
                     /gene="rnc"
                     /locus_tag="VHF05_01870"
     CDS             10400..11092
                     /gene="rnc"
                     /locus_tag="VHF05_01870"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVS79704.1"
                     /translation="MKDFSEFERITGIEFRDKAILKQAFIHRSYLNENKEVGLSHNER
                     LEFLGDAVLELVITDFLYEKYPDKAEGDLTAYRSALVNASTCASVAMNLSVNDFLLLS
                     KGESKDTGRARQYILANTLEAIIGAIYLDQKYDAAREFILKNFSPLIDDIVKMGTWID
                     SKSRFQEKAQEEEGVTPQYKTIRESGPDHDKKFTVGVFLRDKLVAQGDGESKQEAEQK
                     AAAEALQKMGWE"
     gene            11470..>11925
                     /locus_tag="VHF05_01875"
     CDS             11470..>11925
                     /locus_tag="VHF05_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS79705.1"
                     /translation="MKSIELSGFKSFAKKSVLEFNAPISAIVGPNGSGKSNIAESFRF
                     VLGEQSIKSMRGKRGEDLIFNGTPEVPRANRASVKVTFDNSAKFFNIDFPEVTLERVV
                     HRDGANEYFINGSAVRLKDIVELLAQAHIGSSGHHIISQGEADRILNATP"
BASE COUNT         2759 a         3011 c         3249 g         2906 t
ORIGIN      
        1 tcgtccttga atttgtccca gtgcccggaa gtcatgtaga gatccttttt cgtgatatgg
       61 ggaatctcca ctttcatgta gccgcggctc ttgcgcaggc tccatacgaa atcgtcgaga
      121 agattccgga gctccgtgcc cttgggcgtc cagagcggaa gccctgcgcc gacgagatcg
      181 gagaagacga aaaggtcgag ttcgcggccg agcttcttgt ggtcgcgcgc cttcgcctcc
      241 tcgaccattt tttcatacgc ttcgagctcg tccttcgtgt cgaaggcgag gccgtagatg
      301 cgggtgagca ttttgttctt ttcgtcgccg cgccaatacg cgcccgcgat ccgcgagagt
      361 ttccaggaat ctttcgcaat atccttcgcg ggattgtcgg catggccgcc gcggcagaga
      421 tcgaaaaaac cagttgctag tttagagttg ctagttttca gtaaatcgga gagagaagga
      481 gaggaatccc gagggccgga atagtagagg gtaagcggct cacccttgct tgcgatttca
      541 tcgatcagct cgagtttgta cggattgtcg gcaaaaatct tttttgcatc atccgccgcc
      601 gcatctattt tttggaatgt ctgccaggag gggagaagct ccttcatctt cgcttcgatc
      661 ttcgcgagat cctcgtctgc aacgggggaa gcgaactcga aatcgtagta gaaaccgttg
      721 tcgactgccg gaccgagggt attcttggtg ccggggtaga gctcgagaac cgccgcggca
      781 agaagatggg caagcgaatg gcgcttgtgt tcaaggttgt tttccatact gctaaatata
      841 gcatcccgtt agaagtcttc aaacatagtt ttttcgagta cttttgatga ggtggcaggc
      901 ccaaccatgc ctcaaaatac tcatgcgcat gacttctaac gggatagcat ttgagccaca
      961 tttgccaaaa atcagcagta tattgacttt ttaaaccaaa tggtgtataa tgtacatata
     1021 cgctcaaata gggctacttt atgaaaacta tcgccaccac aatgaaatat gcggctctcg
     1081 tcggcatttt cgcgcttgcg ggctttgcaa ctgcttcgac agtcaacgcc gcaggcgaac
     1141 tctccgttac gtgcgcgccc aactactcgt cggtcgtcga aggcgacggc atgaccttca
     1201 cggcatatcc ttcgggcggc accgaactct acaattattc gtggtcgggc gatgacggcc
     1261 tcggcggcgg catacagtcg atcaacgcgg tctacacctc gaccggaacg aaagacgccc
     1321 atgtcgtcgt aacatcgggc agcaagaccg gcgaagcgga ttgcagcgtc gatgttattc
     1381 ccggccagac gtcctcgaac aacacccaga ccaataccaa caccaacggc aacggttcga
     1441 tcttcggcac gaccgacaac ggatcgaatg gcaatccgaa cgtgaccgtc tatacgaaca
     1501 cctccggaca ggtcgcggcg aactacaatg gctcccaata caacggagga cagaccggcc
     1561 agtcgggcgt ctacctctcg caggttccgt acaccggagc gatggacaat ttccatctcg
     1621 cgctctacgc catttccttc tttctctgga gcggcctcgc atcgtactgg attctcaaga
     1681 aaaagtacgg caaggagctc gcgctcgcgg ccgctgggga aaagaatgac gaggacgagc
     1741 tcgcggcgcg catcgtgtcc ctccgtaccg ccgcggctcg ctaaaagcaa attcgcttaa
     1801 aaaaatgagc atccgaagcg caagcttcgg atgcttttgt atgagtgaaa gtacagttag
     1861 cacgccggca gcaaacgctt caagaacgtt ttcagcccgc gtcgtgcggg agggtttttt
     1921 gcgaagcctt cctggacggg ttcttcggta agtctgctca gaacgacttc cggccgtttc
     1981 gttttgaaat catcggcaat ttccttgagc ctgtgccaat ttcgcgcggc aagctcttct
     2041 gcctgcttca gcgtggtgga atcgctgagc attcttacga cgtacctata aagccggaac
     2101 tcgtcgtcgg taggctcgtg caaaaccatc cctgaaccgt tccgggcgag gatcatgatt
     2161 gagggcatat ggggactcct tctctctatc cgttttgacg gatgagaggc gaattgatta
     2221 caccatgagg ccaaaaaccg tcaaaatctc ttctctttgc cccctcattg ctcctcctag
     2281 aaactaaaaa ctagaaacta gaaacttctc ctccgctaca ggagccggac cttgtccgcg
     2341 atcatggaaa ccgagccgtt ccggtgggag atcttgccct tgatgacgac gcagttctcg
     2401 ggcttcaaaa gatcatggaa ttcggcgtag acgcggggaa agaccaccat ttcgacagag
     2461 tccgagaggt cggataccgt gaggaagagc atcttgtcgc ccttcttcgt gtagatgtcc
     2521 ttgcattccg ctatgatgcc gccgacgacc acttcctggc cttccttctt tgtttccttg
     2581 atgagcttga tgttttcgcc gcgagccgcg aacttctcct tgaagcggtc gagagggtgc
     2641 ccggaaatgt agaggccgag gagctctttt tcccatgaca gcttatccga ctggctggcc
     2701 ggcggagcgg agtcgagctt caaggtcgga atggtcgagg tgtcggacat caggccgaaa
     2761 agcgatgttt ggcccgcggc cgtcgtggag ccctctttgt tgtagcggac catcgactcg
     2821 agattggcga gcatctggcc ccgctcgccg aaggcgtcca tggcgccgca cttcacgagc
     2881 gactcgaggg atttcttgtt gaggttgcgg tcggtgacgc ggtcgaggaa gtcggcgagc
     2941 gaggcaaact tgccgccgcg cttgcgctcc tcgatgatgg cgtgggcgat tccttctccg
     3001 aaattcttga tcgtcgttaa tccgaaacgg atcttgtcgc cttcgccgtt cgttttcacg
     3061 acggtaaacc cttcgaacga ttcgttgata tcgggcggca ggacgggaat attcattcgc
     3121 gtgcactcat tgatgacctc ggcgacggtg tcggtgtcgc cggactcggc agtgagcacg
     3181 gccgacatgt agatggcggg gaagttcgct ttcatgtacg ccgtctggta cgcgaccttg
     3241 ccatagctcg cggcatgcgc cttgttgaag ccgtaggcgg cgaacggctc gatgaggctc
     3301 cagagcgtct tggctttttc ctccgagagg ccgtttgcga ggaatccttc gtggagcttc
     3361 gttttttgcg cctccatctc ggcgggaatc ttctttccca tggccttgcg aagcttgtcg
     3421 gcttcgagcc aggagtagcc ggcgagatgg atggcgatga gcatgacgtc gtcctggtag
     3481 gtgatgatgc cgtacgactg gtcgaggatg tccttgaggc gcgggtcgag gtacttcacg
     3541 agcgacgggt cgtgcttgcg cttcacgtac tcggggatca tttccatcgg gccggggcga
     3601 taaagcgcga ccatggcgtt gatatcgaaa atggtcgtgg gcttcagatc catgaggtag
     3661 cgcgtcatgc cggtgccgtt cagctggaag aggccgatcg tttcgccgcg cgcgagcatc
     3721 tcgaaggtct tcttgtcgtc gagcggaata ttttcgatgt cgatatcgat accttcgatt
     3781 tttttcacaa gcgagaccgc gtcggcgagg atggaaaggt tcctgatgcc gaggaagtcg
     3841 aacttcagga ggccggcggc gttctcgtcg accgagtgca tgtcgtactg ggtaatgact
     3901 ttcccttcgc ccttcggatc gagctggagg gggacgtaat cggtgagtgg cgtcggcgag
     3961 atgacgacgc ctgccgcatg cacggagatg tggcgcgcgc agccttcgat cttcttcgcc
     4021 atgtcgatga tttccttggc atcgggatcg ctttgataca tgcgcttcaa gtccggctcc
     4081 atcttgagcg cgcggtcgat ggtcatcggg aagccctggc ttcccatcgg gatgagcttg
     4141 gcgatggtgt cgcccgtgcc gtagggaaag cccaaggcac gcgcgacgtc gcggacggcg
     4201 ccgcgggcca tcatggtgcc gaacgttccg atctgggcga ccttgtcggc gccgtacttt
     4261 tgcttggcgt agctaatcat ctcatcgcgg cggttgtcag cgtagtccat gtcgatatcg
     4321 ggggcggatg ggcgctcggg gttgaggaac ctttcgaacg ggagcttgta gacgagcggg
     4381 ttcacattcg tgatgaacgc gaggaaggta acgagcgagc cgccgaccga gcctcggatg
     4441 gtcgttagga tgccgttttc atgcgcgtgg cggaggagat cggaaaccac aagaaagtac
     4501 tttgcgtagc ctttgtcctt aataaccttg agttcgtatt cgatgcggtc gagcacttcc
     4561 tgggtctttt cgagccctcg cttctcgatt ccttcgaaga cgatgtcgcg gagcacttcg
     4621 tcggcggtgc ggcccgcggg aatctcgatg ttcgggaatt tccattcgcc gagggcgatc
     4681 tcgcaattgc agagctcggc gatcttcgcg gtattctcga gcgcctcggg gagctttttg
     4741 aaatatttct cggcggtctt tccgtcgatg aaggagaaat cctcctgcga ctcgaattcc
     4801 gaaagggcgt ccgaacgcgg gccgccgcca ttcgcgatcg acatgaggac ttcgcgggcg
     4861 cgggcatctt ccttcttcag atagtagaca tcgtgcgcgg ccattatcgg caccccggcg
     4921 ttcttcgcga actcgacgag tttcgccatc gcttcctcgt ggccctggat ctccggatgg
     4981 tgcgtgatct cgatatagaa gtcctcgccg aagacgctct tgtaccactc ggcgagttcg
     5041 cgcgccttct ccaggttgtc gcttttgacc gcgagcgcga tgtcgccgga gaaggaagga
     5101 tttatgcaga tgagcccttc gcggtatttc tcgatgagtt cgcggtcgat gcgcggcttg
     5161 tagtagaagc cttcaagaaa ggaatcggtc accatttgaa gaaggctctt gtagcccgag
     5221 ccgtccttgg cgagaagcac gaggcgcgag cgtgcgttgt cgaggcgcgc ctccttgtcg
     5281 aagcgcgtgc gtgcggcgac gtacgcatcg acgccgatga tgggctttat tccttccttc
     5341 ctgcatttct cgtagaactc gatcgcgccg tagagattgc cgttgtcggt aagggcgagc
     5401 gattcgaaac ccgccttctt cgccgccgcg acaagatcgg gaatctgcgg cagggcgttt
     5461 aggagggagt agtggctatg cgtgtggagg tggacgaact ttgtcataat tcaaaattca
     5521 aaagtaaaaa tgcaaaagtt taactcaaaa ttcaaaaggt ccatacattg accgattttt
     5581 acctctcaat tacagttttg ccttttggat tttgcctttt gacttcgtgc taccatgcta
     5641 ccatttttac gataaaatta atctatgcaa aaacctgtaa aaagggtgaa aaaagcagag
     5701 ttcgtggtgg gtattgatga ggctggaaga gggcctttgg cggggccggt ggcagtgggg
     5761 tcggtcttgg ccggggcggc ggaattgtcg cgattgaggc gggttttcaa atcgataaaa
     5821 ggaaaggatt cgaagaagct caccgaagcg gagcgggagg agtggttcgc cgtcatcaag
     5881 gcggaagcgg atgctggaaa tctcacatgg cgagtttcgc tttcggggag tgcaatgatc
     5941 gacaaaaagg gaattgttcc cgcgattcgc tcggcgcttt ctcgaagctt ggttgcggtc
     6001 gcggatgact cctcgcgcac ccgcgtgctt ctcgacggtg gactcaaagc gcccgcggaa
     6061 ttttcaaatc agaaaacgat cataaagggc gacgaaaagg aactcctcat ttctcttgcg
     6121 tcgatctgcg ccaaggtgac tcgcgacaga tatatgtgcc ggctcgcaaa aaaatacccc
     6181 gaatacggat ttgaggtgca caagggatac gggacgaaga ggcatgcgga aggaataaaa
     6241 tcattcggcc tctgcgcgct gcatcgcaaa agttttataa ggaaaaattt taattttctg
     6301 aaataaaaaa cacctcggct tttgacggcc gaggtgcaga agttgttgtg aaaagaacaa
     6361 tgctaatgta tccaatccca acgggttacc ggtatgtctt tggcgaacat aagccaatct
     6421 tggcctcgag gtgtgtcttc cggctcgagg tcggctgcat tttttgtccg tttgatgcgg
     6481 ccgaatatta ctccggatga tttcgtcccc tggattactt catcagggta ttcccatcta
     6541 tccctttgtg gaatgtgagc gtatcgctcg tcatgacctt cattgtaaac aagcgtaagc
     6601 ccgccgtatt caatttcaac gggggcttgc tgttccgtcg tgctttcagg cattctgtct
     6661 cctttttgat ttccgcagat agtatatcat gcgtagtttg tctacctatt gacccatctg
     6721 gcagtcagat caatccaatc ttgccgaagt ttacttgcat gtgcgcgggc attttcccca
     6781 acaagcatga aaaactgttg cggctcgtct ttcatcattg tttcgatgtc tgaaacaatt
     6841 ttagatgcct taagaagact ctttcccgta agcttcttgc ttgattcgga atacagagga
     6901 acttctcttt tgcttatgga agtgaacttt accggaaatg aaaagacttt cactcctgta
     6961 ttcacctcaa gattgtggat gtagaaatcg aatattccat aatcaacagc attgactcga
     7021 gctctcgatt tcacagcgtc ctctatctca tggtagtcgg tgcgcatctt ccagagattg
     7081 agtgtatttt ctttctcccg ataaaatacg gcacgtttga tcatatcgaa tgttgtgttg
     7141 ttttcctgcc gcaaaccacg aagaatgttc cagagccaga tatcatctcg taatgcaaac
     7201 gaatggatat tattgaggct gaagctttct tttgtgatca taccttgctc aaaagctttc
     7261 tgtatgagga atcgtacaag cattttcatt gcaatgacgc tgtgatggaa atggatccat
     7321 acatgcgtcc gatatcggtc gtagagtaaa gcttccatcg aggtaagagc tttttcggaa
     7381 taagcgagga accagccatt ttcatctctc tcaatgaagg cagagtcgca cagacgtcga
     7441 atatcgtaat gcccatattc acctccagcg agctggccgt cgcgtcggac gaaatcgatt
     7501 ctatcagcat cgatatctga atcaacgatt gattttatta ttgacagagg atcggaagat
     7561 ggaatcgtct tgcgtgtcat aagctgaatg agtgcatttt tgtctggcca ctcattccta
     7621 tggctggatc acttgcaagc tcatttatga tttttgctcc aaaggcttcg tgtagcttct
     7681 cgaaaccttc cgtattagtc cattctttta tcgcctccat tcgtttttgt cgttctgaaa
     7741 tccaatattc cgtcgccgcc atctcaaaca gatggcttag cggaagatgg ccgatgtcat
     7801 gcaatagggc atagaggctt acaagttgca caaattcatc ttgggactcg atgccagcct
     7861 gttttttgag gccatcgaga tattcatccc gtagcggact acggccaaga tgctctgcca
     7921 ttttgctagc aacatgagat gtgccgagca catgctcgaa acgtgatgtg tttacagatg
     7981 gataaacgta gaatgcgacg tcattctggc gaacatgccg taaacgctga aagaatggat
     8041 ggtttataac cctgcgctct aggtcagtaa aataaatggt gccgtgcacc aaatctctta
     8101 ctctaagtgt gaactcaagc attttttgcg atctcctttt tcaaaaaaat tttgatccaa
     8161 aaatataata ctgtggaata tttaatatgt caattttcca aatttattag ccacaaatac
     8221 atgtgccgcc ttgcgaaaaa gtatcccgaa tacggatttg aggtgcacaa gggatacggg
     8281 acgaaaggtc atgtgataca actcaagctt ctggggcctt gcgcttcgca taggatttct
     8341 tttctcaaat aattttagaa aagtaatttt tgactaagtt ataagtatgt gctagtgtaa
     8401 gaaagggagg cactatgcca gtacttactc aagaatttac caagttagag gattgggcga
     8461 gggctcacga tccaagccaa acgaacttgt tgtttaaaaa tggcttctgg gatcagatcg
     8521 aattcgtacg tgatcaaatt ccaggaatcg catacgttgc gtgcggtctc gaagcggctc
     8581 gctacgtcga aacaaatatc agagtcatat caactcatgt ttcaaaatcc gttacgttgc
     8641 ccgtatttca cctgcggctc ccgaacggcg atcagtttgt aatgcgctac aatttccacg
     8701 attggaaagt ctctgtcgcg gcaaaagaac cgatcgatgc tgattttatg agactattca
     8761 atccttcgtt gaaactccat cgggcatatt gcgaaggatt tcctgaagaa tggatttttg
     8821 gatcgtacga ggcagacccg ggaaagttct cggtggtttt gcagtcgggg tattattaca
     8881 ttttcacatt cttctggatt tactttcaga aggtcgtcgc caaacgtata taacgcgcga
     8941 acgaatacgg ctccggtttt tgccggagcc ttttaatttc gattttttga agaaagtagc
     9001 tcatctttat ccccaaatct caaaaaacca ccagttattg ctatttttct ataaatatcc
     9061 tatactgcca aaatatggtt atcaggatga gacacacccg agcgcaccgg gacaatagga
     9121 gggcacatca cgcgctcgaa ggcagcgctt tgaccgtctg cccgaactgc ggagctaaga
     9181 tgcagaggca cacggcatgc cagaattgcg gccagtacaa gggccgggcg gttgtcgacg
     9241 tgcatgcgga actccgcaag aagcaatcga agaagaatgc ttcatccgag acggcggcgg
     9301 agaagtaagc ggggcggagc ggaaagcagc accttgaatt ccaaatcttt ctatggccaa
     9361 taggcacctt tccagatcaa tagttcttca aacgttatac gagtgggatt ttaatcggat
     9421 gcaggacggc gatgcgatgg atattttcca tcggaacgcc gaggaattcg cacccggcat
     9481 gggagacgtg tcgttcatgg aaactctcct caagaacacc ctcgaaaaac ggaaggacct
     9541 cgacttgatc atcgagaaag ccgcgcccga ttggccgctc gacaagatct ccatcatcga
     9601 caggaatatc ctgcgcatcg gcctctacga acttctgttc tcggaccgcg cgcaggtgcc
     9661 gcccaaggtc gccatcaacg aggcgatcga gctcgccaag agcttcggcg gcgaaacctc
     9721 ggggcgcttc gtaaacggcg tcctgggcgc cgtttacaag gagatgggcg agccgggcaa
     9781 ggacgaagtg tcgaagcgca aggacaatcc gggcaaaccg gtgccggtgg agcggcttgc
     9841 cggcgcggtg atcttcgggg tgaaggacgg cgagatccat ctcgcgctcg ttcatgacat
     9901 cttcggccac tggaccctct cgaaagggca cctgacggag ggcgaggagg aagaggcggg
     9961 cgtgaagcgc gtcgtcaagg aagagctcgg catcgacatc gctcccaagt cgaagctcgg
    10021 cgagaacgag tacggaacgt acgatcccga gaagggaaag atcaagaagc atgtgagcta
    10081 cttcctcgcg gagtgcgacg tgtgcgaact cgagcttgcg gaatcgggag gcctcgacaa
    10141 tgccaagtgg ttcccgctcg cggacatcat cgaccttaat ttttacaatg acatttatcc
    10201 gatcgtaacc aaggccgtca atcttctctt gaaggagaac gcggccgcat aattatcaat
    10261 taaataattt tcagttttca atttctaatt ttcaatcaat tttcaagaac tgattttcaa
    10321 gggctgtgct tttggaaatt ggcgcattgg aatttcaatg aaaattgtaa attaaaaatt
    10381 gaaaattctt cagacactta tgaaggattt cagcgaattt gaacggatca ccggaataga
    10441 gttccgggac aaggccatat tgaagcaggc gttcatacac cgctcctacc tgaacgaaaa
    10501 caaggaagtc gggctctcgc acaacgagcg cctcgaattc ctgggcgacg cggtgctcga
    10561 gctcgtcatc accgactttc tctatgagaa atacccggac aaggccgagg gcgacctcac
    10621 cgcatatcgt tcggcgctcg tgaacgcgtc gacgtgcgcg agcgtggcca tgaatctctc
    10681 cgtaaacgat ttcctccttc tctcgaaggg cgagtcgaag gataccggcc gcgccaggca
    10741 gtatattctc gccaacacct tggaggcgat catcggcgcg atctacctcg accagaaata
    10801 cgacgccgcc cgcgagttca tcctcaagaa tttctcgccc ctcatcgacg atatcgtgaa
    10861 gatgggcacg tggatcgatt cgaagagccg cttccaggag aaggcgcagg aggaagaggg
    10921 cgtgacgccg cagtacaaga caatccgcga aagcggcccc gaccacgaca agaaattcac
    10981 cgtcggcgta ttcttgaggg acaagctcgt cgcgcagggc gacggcgaat cgaagcagga
    11041 ggcggagcag aaggccgcgg cggaggcgct ccaaaaaatg gggtgggaat agggtcgctc
    11101 ggaaatctga ttttaagaac acgcataaaa tcgccccagt cgggcgattt ttgctctgct
    11161 cgtcggattt tctgctgaaa atcctcctgc gcaaggtttt aaaatcagaa ttcctcgctc
    11221 tcgtgttctg aaggaacaaa ttttagggtg gagaggataa aatttgtgca gacacattaa
    11281 gattcttggg ctttgatctt gggtaaaact cctcggagtt ttgcctgttt acttaggcaa
    11341 aataaaaccc ctaccggcgc tcacctcctc ccagagtttg gcgccgatag gggatcgact
    11401 agggcaactt attgaggata atactcctta tttcgcaaaa gacaaaatgg tatagtaatt
    11461 acaatgtatt tgaagtctat cgaactctcc ggattcaagt cgttcgccaa gaaaagcgtt
    11521 cttgaattca acgcgccgat ttcggccatc gtcggcccga acggctcggg aaaatcgaac
    11581 atcgccgaat cgttccgctt cgtcctgggc gagcagtcga tcaagagcat gcgcggcaag
    11641 cgcggcgagg acctcatttt caacggcacg ccggaggtgc cgcgcgcgaa cagggcgagc
    11701 gtcaaggtta ccttcgataa ttcggcgaaa ttcttcaata tcgattttcc cgaagtgacg
    11761 ctcgagcgcg tagtgcaccg cgacggcgcg aacgagtatt tcatcaacgg ctcggccgtg
    11821 aggctcaagg atatcgtcga gcttctcgct caggcgcaca tcggctcctc cggccaccac
    11881 atcatctcgc agggcgaggc cgacaggata ctcaacgcca cgccg
//
LOCUS       DASZYS010000032        20313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_678215, whole genome shotgun sequence.
ACCESSION   DASZYS010000032 DASZYS010000000
VERSION     DASZYS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678215"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHF05_01880"
     CDS             <1..220
                     /locus_tag="VHF05_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79706.1"
                     /translation="HINELLKVPNAYDAVNYSTPFMKKALHIPIAFWRQGGTTNPRLQ
                     PIPKARSTGNSERRTASRLFSLFYYEHQ"
     gene            complement(272..1369)
                     /gene="ychF"
                     /locus_tag="VHF05_01885"
     CDS             complement(272..1369)
                     /gene="ychF"
                     /locus_tag="VHF05_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HVS79707.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTKKAVPAENYPFCTIDPSVGIV
                     PVPDERLWKLSEFSKSKKTIPAVVEFVDIAGLVKGASEGEGLGNQFLTNIRETDAIAE
                     VVRIFEDDKVIHVAGKVSPVDDIQVINLELILADLQTVTKRKGNVERDAKRGDKAAVI
                     EAGALAKIEEALQNEKLASSAALDEKEAPLIRQLNLLTMKPILYVLNKKAGGKNLDEM
                     KDPRWSELMAYLDSMNAKWTIVDANIENELKELEGEEKETFRKELGSENDGINNLISK
                     GYELLGLETYFTTGEDETRAWTVKIGSTAPEAGTAIHTDFKEKFIRAEVVHWDELLAA
                     GSYGKARELGKVRTEGKEYIVKDGDVIEFKI"
     gene            complement(1509..2174)
                     /locus_tag="VHF05_01890"
     CDS             complement(1509..2174)
                     /locus_tag="VHF05_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVS79708.1"
                     /translation="MKTPITTVKLYLQVLEKMAKQNKDRETAGHLSKLEIQIDKLTRL
                     VSDLLDLSKIQAGKLELSMKSFDLDDMVGETAEMLQRTVESHLFVLRLGSKRRIRGDR
                     DRLSQVLINFLTNAVKYSPNGDTVIIKTGVKGGKAIVSVTDQGIGIPEEYRAKIFDRF
                     FRIEGDRERTYSGFGIGLYISAEIVNRHGGEICVESMPGKGSTFSFSLPLAAEKKAKA
                     KKR"
     gene            complement(2171..3445)
                     /locus_tag="VHF05_01895"
     CDS             complement(2171..3445)
                     /locus_tag="VHF05_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVS79709.1"
                     /translation="MKKARTSQRKKTSRMPAVVPGADALRSNPFHKVFENANDIVALT
                     DRKGVYLYVSLSMKAHLGYSPRELVGTRGILRIHPDDQKAAHEFLKKLLKKIDGEPIV
                     AEMRAGHKDGTWRWFEVSSNNLLREPSVRAIFSSFHDITERKKNDERYSAFISQSTEG
                     IWRYELPKPVSTKWPAKRQVEYFLKYGRVAECNDAFARMYGYKNAAEVINAGLVRFIN
                     PKDPEHVKLLYAFVASGYRLTDVEVREFDQDGNVKYFLLNLVGVVEGTEGRRAWGTKL
                     DITARKHYENMLKASEERFRNLADQAPVMIWMAGTNNLTYWFNKTWLDYTGRTLGQEI
                     GNGWGEDIHQDDFERFVDVYKRSFEERKAFRIEYRLRRYDGEYHWVIATGTPLFSPDG
                     AFEGFIGTCLDINEIRIIERRRMNSSVSRRMN"
     gene            complement(3509..3742)
                     /locus_tag="VHF05_01900"
     CDS             complement(3509..3742)
                     /locus_tag="VHF05_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79710.1"
                     /translation="MGIKDFLMRKMMERQMQGVPKEEQEKMLTLVQNNPELFQKIATE
                     VQEKMKQGKSQMAATQEVVQKYQDELKKVMDKK"
     gene            complement(4005..4850)
                     /locus_tag="VHF05_01905"
     CDS             complement(4005..4850)
                     /locus_tag="VHF05_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVS79711.1"
                     /translation="MFILFDIGGTKMRVAGSRDGESFGTPKIIPTPASYEEGLAAFKA
                     AVDAVSEGEPVAQIVGGIAGPFSEKTKMLVGSPHLSGWVGKPFADDLRSAFGATVHIE
                     NDAALVGLGEAAYGAGRGFDIVAYVTVSTGVNGARIVAGKIDEKTYGFEIGHQIIDPD
                     KTLAPGAAGIYFEDIVSGTAVEKRMGKKPKEVTDPKFWDEMARVLAIGLNNVVDFWSP
                     EVIVLGGSMITGDPAISVETTEKYLKEYLTIVPELPVMRKAELADVGGLWGALAYLKQ
                     VSEAV"
     gene            complement(4884..6185)
                     /locus_tag="VHF05_01910"
     CDS             complement(4884..6185)
                     /locus_tag="VHF05_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79712.1"
                     /translation="MELHYKATSLVDEDDLMHCADTLEPYLSHLKEVYENLDYEAQEA
                     SLCLPRDGEIMEDVLEAVSAYAETLRFVFVIGIGGSSLGTQAVYEALKSSRSEDDPEL
                     FFIDTMSPSNLRAAAAIIRDELEDSEELLVNIVSKSGKTLETTANAEILLGLLREEFG
                     EVNDRVVVTTGAETPLAEWAKERGIRVLSIPEMVGGRFSVFSPVGLFPLGLAGIDIEA
                     LVRGANVQAEISLDPDLEGNAALVSASAVFLNWAEGSAIHDHFFFAPELEHVGKWCRQ
                     LVAESLGKQFDLEGEEANRGITPTVSIGPNDLHSSLQLALGGPKDKFTTFVRAKAPQA
                     ETFAVPEDADHSFGAAYLQGKTADEFLDAIYEGATDSYSESGLPFIEIDMRELNEHSL
                     GIFLQFKMIETMLLGRLMEVDAFDQPAVESYKKEARRLLGE"
     gene            complement(6231..6947)
                     /locus_tag="VHF05_01915"
     CDS             complement(6231..6947)
                     /locus_tag="VHF05_01915"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVS79713.1"
                     /translation="MILKVNTSSNPAQHGAQKLRELLDNAEGSVLCMLSGGSAFELLQ
                     PFDISKGWERVTITVLDERYSEDEGINNFAQLAKTDFYAKAKSGGASFIDTRIAPSES
                     ENELEVRFDDAVKQWLHGNPGGSIVVTMGIGADGHTAGIFPHEDDEKFRFLFEQDGRF
                     VVGYHAHTAEYHERLTVTNHFLREHVDHAVVFAVGEGKREAVDRVLSAHGLLSETPAR
                     IIREMKDAELFTDISGLAVS"
     gene            complement(7193..8083)
                     /gene="gnd"
                     /locus_tag="VHF05_01920"
     CDS             complement(7193..8083)
                     /gene="gnd"
                     /locus_tag="VHF05_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HVS79714.1"
                     /translation="MKLGYIGLGKMGLNQALRLHEKGHEVLVWNRSEGPRKEAEARGI
                     RSFAALDDLVKSLEAPRTLWIMVPNNAVDEVISQLLPLMSEGDTLIDGGNTYYKDTTR
                     RAEDLRTKGLKFLDAGVSGGPHGARDGACIMIGGEKEDYDRLEPLWRDLSVEKGYAYM
                     GGHGAGHFVKMVHNGIEYGMMQAIAEGFEIMKKSPFSLNLTKVADVYQHGSVVESRLI
                     GWLEQGFKEYGEDLAEITPAVAHTGEGEWTVKAGEEFGVETPVIKASFDFRVHSAEKP
                     SFAGKVLSTLRNMFGGHQAK"
     gene            complement(8185..9642)
                     /gene="zwf"
                     /locus_tag="VHF05_01925"
     CDS             complement(8185..9642)
                     /gene="zwf"
                     /locus_tag="VHF05_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVS79715.1"
                     /translation="MKGVNDPTIFVIFGITGDLSRGKLVPALFDLYLKNRMPAKFHII
                     GFSRRDFSESEFREYVAEVLEYRIGDVDKNTIADFAHHFSYTKGLFEDEAAYKDLAVR
                     LKETDDLFRACSNKLFYLAVPPSYYESILNNLSHSGLTIPCGGDDGWTRVLVEKPFGS
                     NVETARKLDMLLGKLFKETQVFRIDHYLAKETLQNILAFRFSNSIFEPLWNRDYIEKV
                     EINLLEHDTIESRANFYDETGALKDVGQNHLLQMLALIAMEKPDMLFPDTIRQARADV
                     LTNLKRMTRDEAERDTVRGQYEGYQEALHVPGSETETFFSVTARINSKRWKDVPFVLT
                     SGKGLSEDRVEIKIHFKDTSPGSFMPKQYLTQEANQLIFSIKPEEKISILFWIKVPGF
                     ENKIEPKKLTFAYTEGGEMVVVPNAYERVLHDCIAGDQTLFASTAEIEGQWNFIESVL
                     EAWQTLPIHVYKRGSDAGQIERIGKVATGQPYAQY"
     gene            complement(9872..11104)
                     /locus_tag="VHF05_01930"
     CDS             complement(9872..11104)
                     /locus_tag="VHF05_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVS79716.1"
                     /translation="MKDFLERIRKNQTVIAASAISGAIAAVIIVVIAVAFLEWGGGKQ
                     FVSAIGGVPAVATSTPNASLAAGENAVVSTVAKANPSVVSIVISKDVPTVERRFERFG
                     PFSMSVPEQNGGSQEEEVGGGSGFIVSPDGLVVTNKHVVSDKNASYTVFTNDGQKYDV
                     DVVAEDPFYDIAILRIKSDKTFPYLTFANSDNIQLGQTAIAIGNALAEFRNSVSVGVI
                     SGLSRSVTAGDDFGSSETLDQAIQTDAPINPGNSGGPLLNSSGQVVGVNVALVEGSEN
                     IGFALPGNIAKSAVDSVEKTGSIERPYLGVRTIDITPDLAEQEHLAVDHGELVTGGTR
                     ASEAAVVSNSPASAAGIKEGDIILQVDGVNIDDTHTLGELVREHNIGDTVSITLNRAG
                     KQMTLRAVLAKAPQDIAS"
     gene            complement(11321..11935)
                     /locus_tag="VHF05_01935"
     CDS             complement(11321..11935)
                     /locus_tag="VHF05_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79717.1"
                     /translation="MLILALIWSFFGAVAIAIGVEAEATWKKVFKLGAVVDSVRRFLL
                     IGGLIASLATIGCSFYFFVMSNAIIVLIAWIAAFLIARFAVEIMRRRNVYAGTEHAPP
                     PVRSDVTRQAAYAGRREETSDVEEVDSSEPLSPSEARELSPMNAGAAAMSEREAPIRS
                     EAIERPIPSAGGNEAPAPQSPMAPDPDDPSSGAYAEIFKRLNSK"
     gene            complement(12037..12573)
                     /locus_tag="VHF05_01940"
     CDS             complement(12037..12573)
                     /locus_tag="VHF05_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVS79718.1"
                     /translation="MKNGVIQLIRPLRRNPETAESCFERGVDYEHDGDLSKAEYWYKR
                     AARLGSCRAMVNLGTFYYHQNRLEEAELSYRRALAADPQYPLAHFNLANCLEETGQID
                     EAIFHHERALALDPFYANAHFNLARMFEVRGNHMAAYRHWREYAKLDPRSEDGEYARN
                     QMMKMQACYVTNNSTAAS"
     gene            complement(12701..12771)
                     /locus_tag="VHF05_01945"
     tRNA            complement(12701..12771)
                     /locus_tag="VHF05_01945"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12737..12739),aa:Ala,seq:ggc)
     gene            complement(13035..14540)
                     /gene="gpmI"
                     /locus_tag="VHF05_01950"
     CDS             complement(13035..14540)
                     /gene="gpmI"
                     /locus_tag="VHF05_01950"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HVS79719.1"
                     /translation="MNNRRQTLLIVLDGWGHRLETKDNAILNAKKPNFDAMWEKYPHA
                     LLDASSLAVGLPEGQMGNSEIGHMTIGAGTTIDTDLVRIGKAIESGEFAKNPALVALF
                     DHVKQNDSTLHVQGLVSRGGVHSHSEHLFAVLRAARDAGLKRVAVHVFTDGRDTPPQS
                     AAKFLKELEDVLEEVKIGFIASISGRFYAMDRDNNWDRLSKAEQAIFECKGNVCTLKP
                     SEYVASLYTEGKLDEHLEPIVCLGEDGKGCAISQNDAVFFFNFRADRARMLSQRIIEK
                     APEMNLLFATLTEYSNDLKCLVAFPPKSVETTIAKEVSAAGLTQAHIAETEKFPHATY
                     FLNGGVEKPYPGEEHILVPSRKDVPTHDLAPKMRAKEIADHGIEQIEKGTDFIFINFA
                     NVDMVGHTANVPAIVEAVEEVDTQLGRLLETMNKKGGVMFITADHGNAEVNVDQETGE
                     KHTAHTTNLVPAIITDTQYAMHDGTLSDVAPTVLKLLDLPQPVAMTGKSLI"
     gene            14676..14748
                     /locus_tag="VHF05_01955"
     tRNA            14676..14748
                     /locus_tag="VHF05_01955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14709..14711,aa:Ala,seq:tgc)
     gene            complement(14983..16134)
                     /gene="cfa"
                     /locus_tag="VHF05_01960"
     CDS             complement(14983..16134)
                     /gene="cfa"
                     /locus_tag="VHF05_01960"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HVS79720.1"
                     /translation="MNPARKIVEGLLQSADVHIGRGNKGDIYVHDERFYLRVLLQGSL
                     GLGNSYMAGWWTCADLEEFFFKILSARLDQKHVGFAQLVDSILSRFWNLQNARRAFIV
                     GKEHYDLSPKLIWPMLGSSMAYSCSRKSMNSMSLDEGQFAKLDNVCRKLGVVPGDTLL
                     DIGCGWGSLMNVAAREYGAICTGVTVSREQAEYGRSWCKGLPVEFNVSDYRSITGCYM
                     HLASIGMFEHVGVKNYRTYFKTAARLLSDHPRSLFLLHTLATDVSTTTCDPWITRYIF
                     PNGMLPSIAQISRAAEGFFVIEDVENIGAYYHPTLMAWFGNFVSVWPVLRKDFSSWYK
                     GKLKDPEVFYRMWEYYLKSCAAASRARRIQVYQFVLSKRGYPGGYVRPG"
     gene            complement(16241..17218)
                     /locus_tag="VHF05_01965"
     CDS             complement(16241..17218)
                     /locus_tag="VHF05_01965"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HVS79721.1"
                     /translation="MNSGTVSPQHKLRVGVLRGGPSNEYEVSIKTGASVLRNLPEKYQ
                     PQDIFISKQGDWHIDGVARPPEKILQRVDVVWNALHGEYGEDGRVQKLLSDLNMPFTG
                     SDALASAVGMNKLLAKKTFAAYGLKTPFYAAVKRGENTAENAHQMLKSFTLPVIVKPA
                     ASGSSVGVSLAADLVTLIQALEMALAISEVALVEEYIRGKEATVGVIDGFREAKHYAL
                     LPVEIIHGSSFFDYNAKYSPETREVSPGNFSSAESRELEELAVRAHRALGMRHYSRSD
                     FIVSPNGIYILETNSLPGLTEHSLIPKSLETVGSSLPEFLDHVITLAIR"
     gene            complement(17525..18760)
                     /locus_tag="VHF05_01970"
     CDS             complement(17525..18760)
                     /locus_tag="VHF05_01970"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HVS79722.1"
                     /translation="MNIEELKKKVAEFPTEPGVYFFLNGKKNIIYVGKATSLRDRVRS
                     YFGKDLINSRGPLLVQMVEEATDIKFIQTDSALEALILEANWIRKSKPRYNTREKDDK
                     SYNYVIVTKEDYPLITTVRGRELEMAEMTGGLPWKIKYMFGPYPHGLQLREALKIVRR
                     IFPYRDAKCTPLQGRPCFNRQLGLCPGTCTGEISKAEYGKTIRNLKLFFEGKKRTLLS
                     TLEREMKTAAKKQEFEKAGKLRNTIFALTHIRDIALLKREEDITDGPVVRLEAYDIAH
                     ISGTNVVGVMIAVENGEAKKSDYRKFRIKLNPGVNDTAALAEVLSRRLNHQEWQLPTA
                     ILVDGGQAQMNAAKAVLEARDFQIPIVAVTKNDRHRPESFSGDEAFIEANKNAILLAN
                     SEAHRFAIAYHRNLRGRLR"
     gene            complement(19168..>20313)
                     /locus_tag="VHF05_01975"
     CDS             complement(19168..>20313)
                     /locus_tag="VHF05_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79723.1"
                     /translation="AGWLEKLLTAKTNPSDSVTLDYLRGEKKIPLPDARRTQDKGKLQ
                     IRGGNVFNIKNMDVDIPLGKLDVVTGVSGSGKSSFVYEIVYKNLQARLERKYRTAQTF
                     NCASFAGTEYVSRTILIDQSAIGRTPRSNPATYTGAFTFIRDLFAETSEARARGWKAN
                     RFSFNVKGGRCEACQGNGMIEVEMHFLPTVYVTCDVCNGKRFMKETLDVKYKKKSIYD
                     VLEMTIEEGLEFFEDIPAISDRLQTLYDVGLGYLKLGQSATTLSGGEAQRVKISSELY
                     KPHLQKTIYILDEPTIGLHYDDVAKLIEILQKLVDKGNTVLVIEHNMDLIKCADYIID
                     IGPEGGVGGGEVIAKGTPEDVAMNPKSYTGQYLKKVLKVSRSNRKEK"
BASE COUNT         4280 a         5679 c         5560 g         4794 t
ORIGIN      
        1 gcatataaat gaattattaa aagttcctaa tgcttatgat gcggttaatt atagtacgcc
       61 tttcatgaag aaagctctgc atatccccat tgcattttgg cgtcagggcg gtacaactaa
      121 tcccagactc cagccgatac ctaaggccag atcgactggg aatagcgaaa ggagaactgc
      181 ctcaagactt ttttctttat tttattacga acaccaataa aaaagccgca gggacgccaa
      241 cgcccctgcg gctttctatt tttcaaacag attaaatctt aaattctatg acgtcgccgt
      301 ctttgacgat atattccttc ccttccgtgc ggaccttccc gagttcgcgc gcctttccgt
      361 atgagcctgc ggccagaagc tcgtcccagt gaacgacttc cgcgcggatg aacttctcct
      421 tgaagtcggt atggatcgcg gttcccgcct cgggagccgt cgagccgatt ttcaccgtcc
      481 aggcgcgcgt ctcatcctct ccggtcgtga agtatgtctc gaggcccaaa agctcgtagc
      541 cttttgatat caggttgttg atgccgtcgt tctcgcttcc gagctctttc ctgaacgttt
      601 ccttctcttc gccttcgagc tccttgagct cgttctcgat atttgcgtcg acgatcgtcc
      661 acttggcatt cattgaatcg agatatgcca tgagttcgct ccagcgaggg tctttcatct
      721 catcgagatt ttttcctccc gctttcttgt tgagcacgta gagaatgggc ttcatggtga
      781 gaaggttgag ttggcggata agcggagcct ctttctcgtc gagcgcggct gaggacgcga
      841 gcttttcatt ctggagcgcc tcttcaatct tcgcgagcgc cccagcctcg atcacagccg
      901 ctttgtcgcc gcgcttcgcg tcgcgctcga catttccctt ccgcttcgtg acggtctgaa
      961 gatcggcaag aatgagctcg agattgatga cctggatatc gtcgacaggg ctcaccttcc
     1021 ccgcaacatg gattactttg tcatcctcga agatgcgcac cacctccgcg atcgcgtccg
     1081 tttcgcggat gttggtgaga aactggttgc cgaggccctc gccttcggag gcgcccttca
     1141 caagccctgc aatatcgacg aactcgacga ctgcgggaat cgtcttcttt gatttgctga
     1201 attcggagag cttccagagc ctttcgtcag gcacaggaac gatgcccacc gacggatcga
     1261 tggtgcagaa cggataattt tcggccggca cggccttttt cgtcagcgca ttaaaaagcg
     1321 tgctcttgcc aacattcggc aatccgacaa taccaataga aagtgacatg aaaattaaaa
     1381 gtgtttaccc acgcctgccc cgtaggaagc agtcttcggc tttcctgcgg ggttcggaag
     1441 gccgacaatg ccgatagaaa gactcataac gactaacttt atacaaaacc cgcgaaaatc
     1501 tcaatcagtc agcgcttttt agcttttgcc tttttctccg ctgcaagcgg gagggagaag
     1561 ctgaatgtcg agcctttgcc cggcatgctc tcgacgcaaa tctcaccgcc gtggcggttc
     1621 acgatctcgg cggagatgta gaggccgatg ccgaaaccgg aataggtgcg ctcgcgatca
     1681 ccctcgatcc tgaagaagcg gtcgaagatt ttagcgcggt attcctcggg aatcccgata
     1741 ccttggtcgg ttaccgagac aattgccttg ccgcctttca cgccggtttt gatgatgaca
     1801 gtatcgccgt tcggcgaata cttgactgcg ttcgtgagga agtttatgag tacttggctc
     1861 aaacggtcgc ggtcgccgcg aatgcgccgc ttcgacccga gccggaggac gaaaagatgc
     1921 gactcgacag tgcgctgaag catttctgca gtctcgccaa ccatatcgtc gagatcgaag
     1981 ctcttcatcg aaagttcgag cttacccgcc tgaatcttgg agaggtcgag aagatcggaa
     2041 accaggcgcg tcagcttatc gatctggatt tcaagcttgg aaaggtggcc ggctgtttcg
     2101 cgatctttgt tctgcttcgc catcttctca agcacttgaa gatagagctt cacagtcgtg
     2161 atcggcgtct tcaattcatg cgacgcgata ccgatgaatt catcctgcgg cgctcgatga
     2221 tgcggatctc gttgatgtcg aggcaggtgc cgatgaatcc ctcgaaagcg ccgtccggcg
     2281 agaaaagcgg ggtgccggtg gcgatcaccc aatgatattc gccgtcgtag cgccgcaagc
     2341 ggtactcgat ccgaaacgct ttccgctcct cgaaactgcg cttgtagaca tcgacgaacc
     2401 gttcgaagtc gtcctgatga atatcctcgc cccagccatt gccgatttcc tggccgagcg
     2461 tacggccggt gtaatcgagc cacgtcttgt tgaaccaata cgtaagattg ttcgttcccg
     2521 ccatccagat catgacgggc gcctggtcgg ccaaattcct gaagcgctcc tcgctcgctt
     2581 tgagcatgtt ctcgtaatgt ttgcgcgctg tgatgtcgag tttcgtgccc caggcgcggc
     2641 gcccctccgt gccttcaaca acgcccacga gattgaggag aaaatatttc acgttgccat
     2701 cttggtcgaa ttcgcgcact tcgacatcgg tcaagcggta gcctgaggcg acgaacgcgt
     2761 agagaagctt cacgtgctcg ggatctttcg ggttgatgaa tcgcacgagg ccggcattga
     2821 tcacttcggc ggcattcttg tagccgtaca tgcgcgcaaa cgcgtcgttg cattcggcga
     2881 cgcggccgta cttgaggaaa tattccactt gccgcttcgc cggccacttt gtagaaaccg
     2941 gcttcggaag ctcgtagcgc cagatgccct cggtgctttg ggaaatgaag gcgctgtagc
     3001 gctcgtcgtt tttcttgcgc tcggtgatgt cgtgaaagct cgaaaagatc gcgcgcacgc
     3061 tcggttcgcg cagaagattg ttggaggaca cctcgaacca ccgccacgtg ccgtccttgt
     3121 gtccggcgcg catttccgcg acgatcggct cgccatcgat cttcttgagg agttttttga
     3181 ggaattcgtg cgcggccttc tggtcgtcgg gatggatgcg caggatgccg cgggtgccga
     3241 caagctcgcg cggcgaatac cccaggtgcg ccttcattga caggctcacg tagagatata
     3301 cgcccttcct gtcggtcagg gcgacgatat cgttcgcatt ctcgaatacc ttatggaacg
     3361 ggttgcttct cagagcgtcc gcgcccggca cgacagccgg catgcgggac gtttttttcc
     3421 tttgtgatgt gcgtgctttt ttcatgtgcc acatcagggg acgtcttgcg aatacgagac
     3481 atcccctggc gcagaggatg ccgcaggcct atttcttgtc catcaccttc ttaagctcat
     3541 cctggtattt ctgcaccact tcctgcgtcg ccgccatctg gctcttgccc tgcttcattt
     3601 tctcctgcac ttcggtcgcg atcttctgga agagttcggg gttgttctgg acgagggtca
     3661 gcattttctc ctgttcttcc ttgggaacgc cctgcatctg gcgctccatc atctttctca
     3721 tcaaaaaatc cttgattccc atcctgtagt gaaaaatggc attattgctt cgcaatgaag
     3781 ttttgagcca ttttttatgc ttaatgttaa tttttaaaat ccaaaattca aatcatcctg
     3841 taattgccat gccattttct actacaggat cccatggttt tttaaggtaa atcgggtaca
     3901 agaactagta tactaaatta tcctgaggcc ataaaccccg catgaaaaag caccggacgg
     3961 acattcgatg ctcgttttct ttttgcaaat attcaaaata acaattacac cgcttcgctc
     4021 acctgcttca aatatgcgag cgcgccccag aggccgccga cgtcggcaag ctccgccttt
     4081 cgcatgacgg gaagctcggg cacgatcgtg aggtattcct taaggtattt ttcggtcgtt
     4141 tcgacggaaa tcgccgggtc gcccgtgatc atcgagccgc cgagtacgat gacttccggc
     4201 gaccagaagt cgacgacgtt attgaggcct atcgcaagca cgcgcgccat ctcgtcccaa
     4261 aatttcggat cagtcacttc cttcggcttc ttccccatgc gcttctcgac ggccgtgccc
     4321 gacacgatat cctcgaaata gatccccgcg gcgccgggcg cgagcgtttt gtcgggatcg
     4381 atgatctgat ggccgatttc gaagccgtag gttttctcgt cgatcttgcc cgcgacgatc
     4441 cgcgcgccgt tcacgcccgt cgacaccgta acatacgcga cgatgtcgaa gccgcgccct
     4501 gcgccgtatg ccgcttcgcc gagccccacg agcgccgcat cgttctcgat atgcactgtc
     4561 gcgccgaatg cgctcctcaa atcatcggca aacggctttc cgacccatcc ggaaagatgc
     4621 ggactgccga cgagcatctt cgtcttctcg gaaaacggcc ctgcgatgcc gccgacgatc
     4681 tgggcgacgg gctcgccctc ggaaaccgcg tcgactgccg ccttgaatgc cgcaagcccc
     4741 tcctcgtacg aagcgggagt cggaataatt ttcggcgtgc cgaagctctc gccgtcgcgc
     4801 gatcccgcga ctctcatttt tgttcctccg atatcgaaaa gaataaacac ggtaattagt
     4861 acttagtagt taaatcttag aagttactct cccaagaggc gtctcgcctc cttcttatac
     4921 gattccaccg caggctggtc gaacgcgtcg acttccatga ggcgcccgag aagcatcgtc
     4981 tcgatcatct tgaactggag gaagatgccg agcgaatgct cgttcaattc tctcatatcg
     5041 atctcgatga acggcaagcc gctctccgaa tacgaatcgg tggcgccttc gtaaatggcg
     5101 tcgaggaatt cgtccgccgt cttgccctgg agatatgccg cgccgaacga atgatccgcg
     5161 tcttccggca cggcgaatgt ctcggcctgc ggcgccttcg cgcgcacaaa cgtcgtaaat
     5221 ttgtctttcg ggccgccgag cgcgagctgg aggctcgaat gaaggtcgtt cggtccgatg
     5281 gaaacggtcg gcgtgatgcc gcggttagct tcttcgccct cgaggtcgaa ctgcttgccg
     5341 aggctttccg ccaccagctg gcggcaccat ttgcccacat gctcgagctc cggagcgaaa
     5401 aagaaatggt catggatggc cgagccttcc gcccagttga gaaaaacggc cgacgctgag
     5461 acgagggcgg cattgccttc gagatcggga tcgagggaaa tttccgcctg gacattggct
     5521 ccgcggacaa gcgcttcgat atcgatgccg gcaagcccca ggggaaagag tcccaccgga
     5581 gaaaaaacgg agaacctgcc gccgaccatt tcgggaatgc tcaagaccct gatacctctt
     5641 tcttttgccc actcggcaag gggcgtctcg gcgccggtgg tcaccaccac gcgatcgttc
     5701 acctcgccga attcctcgcg aagcaatcca agaaggattt ccgcattcgc ggtagtttcg
     5761 agcgtttttc ccgacttgct cacgatgttc acgaggagtt cctcggagtc ttccagctcg
     5821 tcgcggataa tcgccgccgc ggcgcggaga ttcgagggcg acatggtgtc gatgaagaag
     5881 agctcgggat cgtcctcgga gcgcgaggat ttgagcgctt cgtagacggc ctgggtgccg
     5941 aggctcgagc cgccgatgcc gatgacgaac acgaagcgga gcgtctcggc gtatgccgaa
     6001 acggcctcca gcacatcttc cattatttcc ccgtcgcgcg gcaggcagag cgaggcctcc
     6061 tgcgcttcgt agtcgagatt ttcatagacc tccttgagat gcgaaagata cggctcgagg
     6121 gtatcggcgc aatgcatgag atcgtcttcg tcgacaagag atgttgcctt gtagtgaagt
     6181 tccatacgat atgcttatta tatcgcaaca ttgccgctgc ttcgggcttc tcaagaaacg
     6241 gcaaggccgg aaatatcggt aaaaagttcc gcgtctttca tttcgcggat gatccgtgcc
     6301 ggagtttcgg agagtaagcc gtgcgccgag agaacgcgat cgaccgcttc gcgcttccct
     6361 tctcctaccg cgaaaactac cgcgtgatcg acatgctcgc gaaggaaatg attcgttacc
     6421 gtgaggcgct cgtgatattc ggcggtatgc gcatgatacc cgacgacgaa tctcccatcc
     6481 tgctcgaaga gaaagcggaa tttctcgtcg tcttcatgag gaaaaattcc cgcggtgtgg
     6541 ccgtcggcgc ctatacccat cgttacgacg atactgccgc cgggatttcc atggagccac
     6601 tgcttcaccg cgtcatcgaa gcgcacttcc agctcgttct ctgattcgct cggcgcaatg
     6661 cgggtatcga tgaagcttgc gccgccgctt ttcgctttgg cataaaaatc ggtcttcgcg
     6721 agctgggcga aattgtttat tccctcatcc tcgctgtatc gctcgtcgag caccgtgatc
     6781 gtaacgcgct cccaaccttt cgatatatcg aaaggttgga gaagttcgaa tgccgaaccg
     6841 ccggaaagca tgcacaaaac cgaaccttcc gcattatcca aaagctctcg aagcttttga
     6901 gccccatgtt gagctggatt tgaagatgta ttgactttta atatcattag tgttattatt
     6961 aaacaaggag agcagagatg ttcgcaacaa gtaccaatag gttggttata ctcgcagccg
     7021 ctgtaatgat attgattttt ctgtcgatga agttttcgac ggtggaaaaa acgattcaca
     7081 gcggctggca gtcagcaagc cagcatttcg agcagaagct cgaaggccgc aagtaagatg
     7141 cggccgccat caggcgcgac gactttcact gtcatcgcgc tttcatttta tcttatttcg
     7201 cctgatgccc tccgaacata ttacgcaagg tggaaaggac cttgccggca aacgagggct
     7261 tctctgccga gtgcacgcgg aaatcgaatg aggcttttat aaccggcgtc tcgacgccga
     7321 attcctctcc ggccttcacc gtccactccc cttcgccggt gtgcgcgaca gccggggtga
     7381 tttccgcgag atcctcgccg tactccttga acccctgctc gagccagccg atgaggcggg
     7441 actccaccac agagccgtgc tggtagacat ccgccacttt ggtgagattg aggctgaaag
     7501 gactcttctt catgatctcg aagccttcgg caatcgcctg catcatgccg tattcgatgc
     7561 cgttgtggac catcttcacg aagtgacccg cgccgtggcc gcccatatac gcgtatcctt
     7621 tttccactga aagatccctc cagagaggct cgagccggtc gtagtcttcc ttctcgccgc
     7681 ctatcatgat gcacgcccca tcccgcgccc cgtgcggccc gccggaaact ccggcgtcga
     7741 ggaatttcaa tcccttggtg cggagatcct cggcgcgccg cgtcgtgtcc ttgtagtacg
     7801 tattgccgcc gtcgatcagc gtgtcgccct cgctcataag cggcagaagc tgtgaaatca
     7861 cttcatcaac tgcattgttc ggcaccatga tccagagcgt gcgcggcgcc tcgagcgatt
     7921 tcacgaggtc gtcgagcgcg gcaaacgagc ggatgccgcg ggcttccgcc tccttccgcg
     7981 ggccttcgct cctgttccac acgagcacct cgtggccttt ttcatgcagg cgaagagcct
     8041 ggttcaatcc catttttccg aggccgatat accctaactt cataataaat aatttttaat
     8101 tttcaatttc aaatttccaa tgtttgaaaa attcattatt gcaaattgat tgaaaattga
     8161 gaattgtaaa ttggaaattg ccgcttagta ctgcgcgtac ggctgtcccg tcgccacctt
     8221 gccgatgcgc tcgatctggc cggcatcgct gccgcgcttg tagacatgga tcgggagcgt
     8281 ttgccacgcc tcgagaaccg attcgatgaa attccactgg ccttcgatct cggcagtcga
     8341 cgcgaaaagc gtctggtcgc cggcgatgca gtcgtggagc acgcgctcgt aggcgttcgg
     8401 caccacgacc atctcgccgc cttcggtgta cgcgaaagtg agcttcttcg gctcgatctt
     8461 gttttcgaag cccggaacct tgatccagaa caggatggaa atcttttctt cgggcttgat
     8521 ggagaatatg agctggttcg cctcctgggt gagatactgc ttcggcatga acgagccggg
     8581 gctcgtatcc ttgaaatgaa tcttgatctc cacgcggtct tccgagaggc ccttgccgga
     8641 ggtgaggacg aacggtacgt ccttccagcg cttgctgttg atgcgcgcag tgacggagaa
     8701 gaacgtttcc gtctccgagc ccggtacgtg aagcgcttcc tggtagcctt cgtattgtcc
     8761 gcgcaccgtg tcgcgctcag cctcgtcgcg cgtcatgcgc ttcaggttcg tgaggacgtc
     8821 ggcgcgcgcc tggcggatcg tatcgggaaa gagcatgtcg ggcttttcca tggcgatgag
     8881 cgcgagcatc tggagaagat ggttctggcc cacgtccttg agcgcccccg tttcgtcgta
     8941 gaagttggcg cggctttcga tcgtgtcgtg ctcgaggaga ttgatctcga ctttctcgat
     9001 gtagtcgcgg ttccaaagcg gctcgaagat gctgttcgag aacctgaagg ccagaatgtt
     9061 ctggagcgtc tctttcgcca gatagtgatc gatgcgaaag acttgggttt ccttgaagag
     9121 cttgccgaga agcatgtcga gcttgcgggc ggtctcgaca ttcgagccga agggtttctc
     9181 gacgaggacg cgcgtccagc cgtcgtcccc gccgcacgga atggtgaggc ctgaatgcga
     9241 gagattgttc aggatcgact cgtagtagct cggcggcacg gcgagataga agagcttgtt
     9301 cgagcaggcg cggaaaaggt cgtcggtctc cttgagacgg accgcgagat ccttgtacgc
     9361 cgcttcatcc tcgaaaaggc ccttggtgta gctgaaatga tgcgcaaaat cggcgatcgt
     9421 attcttgtcg acgtcgccga tgcggtattc gaggacttcg gcgacgtact cgcggaattc
     9481 cgattcggaa aaatcgcgcc gcgagaagcc gatgatgtgg aacttcgcag gcatcctgtt
     9541 cttgaggtaa agatcgaaga gcgccggaac gagcttgccg cgcgagagat cgcccgtgat
     9601 gccgaagatg acgaagatag tcggatcgtt gacgcctttc atcccgttag aagcagatcg
     9661 caatcgtcgc gttttctgca gaattaattt tgcatgttaa aaagtcgacc attttatttg
     9721 tcatgatcca tttcagcgat cgcgtgctct caacgggatc atattggtac cagtatatca
     9781 ctatcgcgag ccgcctgaga attcacagac gggagaaaga cgccaaaacg ccggccgcga
     9841 aatcgcggcc ggcgtttttc gtattgtatg tttatgacgc gatgtcctgc ggcgccttgg
     9901 cgagcacggc tcggagcgtc atctgcttgc ccgcgcgatt caacgtgatg gaaaccgtat
     9961 cgccgatatt atgttcgcgg accagttcgc cgagcgtatg ggtatcatcg atattcacgc
    10021 cgtccacttg gagaatgatg tccccttcct tgatgcccgc cgcggaagcc ggagaattcg
    10081 aaacgacagc ggcttcggac gcccgcgttc cgccggttac cagctcgcca tgatcgacgg
    10141 caaggtgctc ctgctccgcg aggtcgggag tgatatcgat agtgcgcacg ccgaggtacg
    10201 gccgctcgat cgagcccgtc ttctcgacgg aatcgaccgc cgacttggcg atattgcccg
    10261 gaagggcgaa gccgatattt tcggagccct cgacaagagc cacattcacg cccaccacct
    10321 gtcccgatga attgaggagc ggaccgcctg aattgcccgg gttgatcggc gcatcggtct
    10381 ggatggcctg gtcgagcgtc tcggaactgc cgaaatcgtc ccccgcagtc accgagcgcg
    10441 agaggccgga aataacgccg actgaaacgg agttcctgaa ttccgcaagc gcattgccga
    10501 tagcgatcgc cgtctggccg agctggatgt tgtccgaatt cgcgaaggtg agatacggga
    10561 atgttttgtc gctcttgatg cggagaatgg cgatgtcgta gaacgggtct tcggcgacga
    10621 catcgacgtc gtacttctgc ccgtcattgg taaataccgt atatgatgcg ttcttgtcgg
    10681 aaacgacgtg cttgttggtc actacgaggc cgtcgggaga gacgatgaag cccgaaccgc
    10741 cgcccacttc ctcttcctgc gaccccccgt tctgctccgg caccgacatg ctgaaggggc
    10801 cgaaacgctc gaatcggcgc tcgactgtcg gcacgtcctt ggaaatgacg atcgaaacga
    10861 ccgacgggtt cgctttggcg acagtcgaaa caacggcatt ctctccggcg gccagggaag
    10921 cgttcggggt cgatgtggcg accgccggaa cacctccgat cgcgctcacg aattgcttgc
    10981 cgccgcccca ctccagaaac gcgaccgcaa tgacgacgat aatgaccgcg gcaatggcgc
    11041 ccgagattgc cgatgccgcg ataaccgtct ggttttttct tatcctttcc agaaaatctt
    11101 tcataggatg aaatatttga atgaaatatt ttgaaaatcg gcctagatgt tcgaggaaaa
    11161 gccgcgtgca acggactgtg cggatatgca gcctccttga gcctgacctg cctacttatt
    11221 acaatccgcc gcaccgatta ttacttcagc ctcgagaggc tacgcacctg agcgtagact
    11281 ccggcagtga atattgaatg aaagaatcct gaggcaaggc ctacttggaa ttgagccgct
    11341 tgaagatttc cgcataggcg ccggagctcg gatcgtccgg gtcgggagcc atgggagact
    11401 ggggggccgg cgcttcattt ccgcctgcgg aaggtatcgg tcgttcgata gcttccgaac
    11461 ggatcggggc ttccctctcg ctcattgcgg cggcacccgc gttcataggc gacaactcgc
    11521 gcgcttccga cggagacagc ggctcggacg aatcgacttc ctcgacatcc gatgtttcct
    11581 ctcttcttcc cgcgtatgcc gcctggcggg tcacgtccga gcgtacggga ggaggcgcat
    11641 gttccgttcc ggcatacacg tttctgcggc gcatgatttc aaccgcgaac cgcgcgatga
    11701 gaaatgccgc gatccacgcg atgaggacga tgatggcgtt gctcattacg aaaaaataga
    11761 acgagcagcc gatggtcgca aggctcgcga tgaggccgcc gatgagaagg aagcggcgca
    11821 cgctgtcgac caccgcaccg agcttgaaga cttttttcca cgtggcctca gcctcgacgc
    11881 cgatcgcaat cgcgactgcg ccaaagaagg accagataag ggcgaggata agcatggatg
    11941 gcctgattcc tgatatgcgc taagtatacc atgaggaatt atcgcttgag gtaagcattt
    12001 cctataaagt tcggattgat aaaaaagcgg ctccctttag gaagccgccg tagagttatt
    12061 tgtaacataa cacgcttgca ttttcatcat ctgattgcgg gcatactctc catcttcact
    12121 gcgaggatca agttttgcat attctctcca atgacgatat gccgccatat gatttcccct
    12181 gacttcaaac atgcgtgcca gattgaaatg cgcattcgca tagaatggat cgagtgcaag
    12241 cgccctctca tgatgaaaaa tagcttcatc gatctgtcct gtttcttcga gacaattcgc
    12301 gagattgaaa tgtgcaagcg gatactgcgg atcggcagcg agcgccctcc tgtaggaaag
    12361 ttccgcttct tcaagtctgt tttgatgata ataaaacgtt ccaagattta ccattgcacg
    12421 acaacttcca agccgggccg ccctcttgta ccaatactct gccttgctta aatcgccgtc
    12481 atgttcgtag tcgacgccac gctcgaaaca actctccgca gtttcaggat ttcgccgcag
    12541 agggcgaata agctggatga cgccgttctt catccgccga aatcgagcct tctccatgca
    12601 aatccccttt ctggtttaat aatacacacg agaaaacagg gagcaagagc aagcagaata
    12661 tgcacaaaat cccgcgcagg cgggattgtt gtgtcgtctg tggacctacg gggagtcgaa
    12721 ccccgacttc gtccatgcca tggacgcgtt ctaccgctat actataggcc cttaacaccc
    12781 ggcattatag caaaaaatgc tttgttgaaa agttggacga cccagaactt tcagatttgg
    12841 atgcggttca aggcggccgc gagtgagaaa gtgaaggcgt ggagatttcg caaggccgga
    12901 gcccgatggg gcgaggcggg ctcgcgggat ttgccaccag gcaaatatcc gtgagtcgcg
    12961 accaaagtgt acatgctcgt actctgcggg aacgaacgga gcggcccagc tgaagcgcgc
    13021 cagatatgga aattctagat aaggctcttt cccgtcatgg caacaggctg aggaagatcg
    13081 agaagcttca gaaccgtcgg cgcgacatcg gagagcgtgc catcatgcat tgcgtactgt
    13141 gtgtcggtga tgatcgccgg cacgagattc gtcgtatgcg cagtgtgctt ctcccccgtc
    13201 tcctgatcga cgttcacttc ggcgttgccg tggtcggcgg taatgaacat cactccgccc
    13261 tttttgttca tcgtttcaag gagtctgccg agctgggtat cgacttcctc gacggcttcg
    13321 acgatcgcgg gcacgttcgc cgtgtgcccc accatatcga cgttcgcgaa gtttataaaa
    13381 atgaaatcgg ttcccttctc gatctgctct attccatgat cggcgatttc cttggcgcgc
    13441 atctttggag ccaagtcgtg cgtcggcacg tccttccgcg agggaacgag aatatgctct
    13501 tcgcccgggt acggcttctc gacgccgccg ttcagaaaat acgtcgcgtg cgggaatttt
    13561 tccgtctcgg caatgtgcgc ttgagtgagc cccgccgcgg atacctcctt ggcgattgtc
    13621 gtttcgaccg acttcggcgg aaaggctacg aggcatttga gatcattgct gtactctgtg
    13681 agcgtcgcga agagaagatt catctcggga gctttttcga tgatgcgctg tgagagcatg
    13741 cgggcgcggt cggcgcggaa gttgaagaag aacaccgcgt cgttctgcga gatggcgcag
    13801 cccttgccgt cttcgccgag gcacacgatc ggctccagat gctcgtcgag cttgccttcg
    13861 gtgtagagcg acgcgacgta ttcagagggc ttgagcgtgc agacattgcc tttgcattcg
    13921 aatatcgcct gctcggcctt ggaaagccgg tcccagttgt tgtcgcggtc catggcgtag
    13981 aagcggccgg agatgctcgc aatgaatccg atcttcactt cctcgagcac gtcttcgagc
    14041 tccttcagga acttggcggc gctctgcgga ggcgtgtcgc ggccgtcggt aaagacgtgc
    14101 accgctacgc gcttcaaccc cgcgtctctc gcggcgcgaa gcactgcgaa gaggtgctcg
    14161 ctgtggctgt gcacgccgcc gcgcgacaca aggccctgca catggagcgt cgagtcgttc
    14221 tgcttcacat gatcgaagag cgctacgaga gccgggttct ttgcgaactc gccgctttcg
    14281 atcgccttgc cgatgcgcac gagatcggtg tcgatagtcg ttcccgcgcc gatcgtcatg
    14341 tggccgatct cgctgttgcc catttgtcct tcgggaagcc ctaccgcgag cgagcttgcg
    14401 tcgagaagcg catgcgggta cttctcccac atggcatcga aattcggctt tttggcattg
    14461 agaatcgcgt tatccttcgt ttcgaggcgg tgtccccatc cgtcgaggac gataaggaga
    14521 gtctggcggc ggttgttcat atcaatacga gaaattaaaa gctttttgac gggagtattt
    14581 tcgcatattt gggaggaggg ttcaaattaa aaagctgaat aaacgcttgc tattacttaa
    14641 tttgtacact ataatatttt ctcgataata ggagcgggcc cttagcttag ttggtagagc
    14701 gcctcatttg caatgaggag gtcaccagtt cgaatctggt agggtccacc gagacaaaaa
    14761 acagtgcttg tgcactgttt tttcgcaagg tggaaaagcg gaggcatatt tctgcagcag
    14821 cagaaatgcc gagctgggat cgagagacca gacttgccag tcgcaagtct ggaactcgtg
    14881 accacaacgg tataccagcc ctgcggctga ttttttgcaa ataaaatccg cactgcgata
    14941 tctcgcagtg cggttttttt agcttaacgc ttgccgagcg tgtcatcccg gacgaacgta
    15001 gcctccggga taaccgcgtt tcgaaagaac gaattgatat acttggatgc gccgtgcgcg
    15061 ggaagcagcg gcgcaggatt tgaggtaata ctcccacatc cgataaaaga cttccggatc
    15121 tttcagcttt cctttgtacc aggaactgaa gtctttccgc aacacaggcc aaacagacac
    15181 gaagtttcca aaccacgcca taagcgtcgg atgataatac gccccgatgt tctctacatc
    15241 ttcgatgacg aagaatcctt ccgccgcccg gctgatctgc gcaattgaag gcagcatccc
    15301 gttcgggaaa atatacctgg tgatccacgg atcgcacgtt gtcgttgaaa cgtccgtagc
    15361 aagcgtgtgg agcaagaaga ggcttcgcgg atgatccgaa agcaatcgcg ccgcagtttt
    15421 gaaatacgtg cgataatttt tcacgcccac atgttcgaac atgccgattg atgcaagatg
    15481 catataacac cctgtaatgc ttcgatagtc gctcacattg aattcgactg gcagaccctt
    15541 gcaccaactc ctcccatatt cggcctgctc ccgggaaacg gtaacgccgg tgcatatcgc
    15601 gccatattct ctcgcggcga cgttcatgag gcttccccag ccgcagccga tatcgagaag
    15661 cgtgtcgcca ggcacgacgc cgagcttcct gcacacgttg tcaagcttgg cgaattgtcc
    15721 ctcatcgaga ctcatggaat tcatgctttt tcgggaacat gaataagcca ttgaagaccc
    15781 gagcatcggc caaattaatt ttggactcaa gtcgtagtgt tctttgccca cgatgaacgc
    15841 ccgcctcgca ttttgcagat tccagaatcg tgaaagaatc gagtcaacta attgtgcgaa
    15901 tccgacgtgc ttctgatcga gccgcgccga tagaatcttg aagaagaatt cctcgagatc
    15961 ggcgcatgtc caccagccgg ccatgtacga attaccgaga cctagcgagc cttggagaag
    16021 cacccggaga taaaatcttt cgtcatggac atagatgtcg cccttgttac ctcttccgat
    16081 gtggacatcg gcagactgga gcagtccttc gactatcttt cttgcgggat tcatatccct
    16141 cctttgaaat gaattgtacg gtacgtccgg tagtaatatg caattaaaat aataaaaagt
    16201 caacggcctg ccgcacggca ggccgttgtt gaagaaaaaa ctagcgaatc gcgagcgtga
    16261 tgacgtggtc gagaaattcg gggagcgagg agccgactgt ctcgagggat ttcgggataa
    16321 gcgagtgctc ggtaaggcca ggcagagaat tggtttcgag aatatagatt ccgttcggag
    16381 agacgatgaa gtcggagcgg gagtagtggc gcatgccgag cgcccggtgg gcgcggacgg
    16441 cgagctcctc gagctcgcgg ctctccgccg agctgaagtt gcccgggctc acttccctcg
    16501 tctcgggact gtacttcgcg ttgtaatcga aaaacgaaga cccgtggatg atctcgacag
    16561 gaaggagcgc gtaatgcttc gcctcgcgga agccgtcgat cacgccgacc gtcgcctcct
    16621 tgccgcgtat gtattcctcg acaagcgcga cttcggaaat ggcgagtgcc atctcgagag
    16681 cttgtatgag cgttacgaga tcggccgcaa gcgagacgcc cacggaagag ccggaagcgg
    16741 caggcttcac gatgaccggc agagtgaacg acttgagcat ctgatgcgcg ttttccgcgg
    16801 tattctctcc ccgcttcacc gccgcgtaaa acggcgtctt cagaccgtag gcggcgaagg
    16861 tctttttcgc aagaagcttg ttcatgccga cggcggacgc gagcgcgtcg gagccggtga
    16921 acggcatgtt gagatcggaa aggagcttct gcacccggcc gtcttcgccg tactcgccat
    16981 ggagggcgtt ccagaccaca tcgacgcgct ggaggatttt ttcgggcggg cgggcgacgc
    17041 cgtcgatgtg ccagtcgccc tgcttggaaa tgaaaatgtc ctgcggctgg tacttttccg
    17101 gaaggttgcg caacacgctc gcgccggtct tgatggagac ttcgtattca ttggacgggc
    17161 cgccgcgaag aacgccgacg cgcagcttat gctgtggaga tacggtgcca gaattcatgc
    17221 actcattata tatgcaatgc gcgctttgaa gaagatccgc ggaatgcggg cgcaataaag
    17281 ccccgcagtt tccccgcggg gccgccggcg actctggcaa actactcatc cagttgccaa
    17341 acttggtgtc cgcttgagtg aatgccggct gttccgcaag catatcctcc tcatatgaaa
    17401 aatgaattaa aaaaagaggc ctcgaagcaa tttgctccga ggccataaaa tattaggaag
    17461 cttggaggag aaataaactt ccagccttac catactaaat atcgcgttca catacaagcc
    17521 gctcttatcg aagcctcccg cgaagattgc gatgatacgc aatggcaaag cgatgagctt
    17581 cgctgttggc gagaaggatg gcgttcttgt tcgcctcgat aaacgcttcg tcgcccgaga
    17641 agctctccgg cctatggcgg tcgttcttgg tgacggcgac aatcgggatc tggaaatccc
    17701 gcgcttcgag caccgctttc gcggcgttca tctgagcttg gccgccgtcg acaaggattg
    17761 cggtcggaag ctgccattcc tgatggttca gccttcgcga gagcacttca gcgagcgccg
    17821 ccgtgtcgtt cacgccggga ttgagcttaa tgcggaattt tcggtaatcg gatttcttgg
    17881 cttccccgtt ctcgaccgcg atcatgacac cgacgacatt cgtgccggaa atatgcgcga
    17941 tgtcgtacgc ttcgaggcgc accaccggcc cgtcggtaat atcctcctcg cgcttcagga
    18001 gcgcgatgtc gcggatatgc gtgagcgcga atatcgtatt gcggagcttt cccgccttct
    18061 cgaattcctg ttttttagcg gcagttttca tttccctctc aagcgtgctc aaaagggtgc
    18121 gcttcttgcc ctcgaagaag agcttgagat tgcggatcgt ctttccgtat tccgctttcg
    18181 agatttcgcc ggtgcacgtg cctgggcaga gaccgagctg gcggttgaag catgggcggc
    18241 cctggagcgg cgtgcatttc gcgtcgcggt acgggaagat cctccgcacg atcttcaggg
    18301 cctcgcggag ctggagcccg tgcggatacg gcccgaacat atatttgatc ttccacggca
    18361 ggccgccggt catttcggcc atttcgagct cgcgcccgcg cactgtcgta atgagcggat
    18421 aatcctcctt ggtgacgatg acgtagttgt agctcttgtc atccttctcg cgggtattgt
    18481 atcgcggctt cgacttgcgg atccagttgg cttcgaggat gagcgcctcg agcgcggagt
    18541 cggtctggat aaacttgata tcggtcgctt cctccaccat ctgcacgaga agcggcccgc
    18601 ggctatttat aagatctttg ccgaaatagc tccgcacgcg gtcgcgcaag ctcgtcgcct
    18661 tgccgacgta aataatgttt ttctttccgt tcaaaaagaa atacaccccc ggctcagtcg
    18721 ggaattcggc cactttcttt ttgagttcct cgatattcat cccgttagag ataggaaacg
    18781 gcagccgttt ccgttgctgt ttacgatatc gtttaaaata cgaacccgct agatttaacc
    18841 ttttgaagcg gttttaccac atgcataatc tctaacggga ttcatgcgct tgtattgtag
    18901 caattttgac aatttgcgct atctcaacaa taatgttgag aggagtacac cattgtgcaa
    18961 tttcccaacc agccgataac ctcagcaaga caagaagtcc tttttccttc ttcctatcca
    19021 ttgcttgaaa atgaagagca agaggagccc ccggaagccg ccgaagctct cgaatatttt
    19081 cataacaacg gcaccggcga ctgaaaacac ccgcaccgca aagcctgtcc gacgctcacg
    19141 cgtccggcag gcttttcctt ttagaattta tttctccttc cgattggaac gcgacacctt
    19201 caatactttc ttcaaatact gcccggtgta ggacttcgga ttcatcgcca cgtcttcggg
    19261 cgttcccttg gcgatgactt ccccgccgcc cacgccgcct tcgggcccga tgtcgatgat
    19321 atagtcggcg cacttgatga ggtccatgtt gtgctcgatc acgagcacgg tattgccctt
    19381 gtcgacgagc ttctggagaa tctcgatgag cttcgccacg tcatcgtagt ggaggccgat
    19441 cgtcggctcg tccaggatgt agatcgtctt ctggagatgc ggtttgtaaa gctctgagga
    19501 aatcttcacg cgctgggctt cgccgccgga gagcgtagtc gcggactggc cgagcttcaa
    19561 gtatcccaag ccgacgtcgt agagcgtctg aagcctgtcg gaaatcgccg gaatgtcctc
    19621 gaaaaattcg agcccttcct cgatcgtcat ctcaagcacg tcgtagatgc tctttttctt
    19681 gtacttcacg tcgagcgtct ctttcatgaa ccgcttgccg ttgcagacat cgcaggtcac
    19741 gtagacagtc ggcaaaaagt gcatttcgac ttcaatcatg ccattgccct ggcaggcttc
    19801 gcagcggccg cccttcacat tgaacgagaa gcggttcgcc ttccagccgc gcgccctcgc
    19861 ttccgacgtt tccgcgaaca agtcccgaat aaaggtgaac gcgccggtgt acgtcgccgg
    19921 gttcgagcgc ggcgtacgcc cgatggccga ctggtcgatc aagatcgtgc gcgacacgta
    19981 ttccgttccg gcgaacgacg cgcagttgaa agtctgcgcc gtacggtact tgcgctcgag
    20041 gcgcgcctga agatttttgt agacgatctc gtagacgaat gacgacttgc cggagcccga
    20101 cacgccggtc accacgtcga gcttgccgag cgggatgtcc acgtccatgt tcttaatatt
    20161 gaaaacgttg ccgccgcgga tttggagctt tcccttgtcc tgcgtcctgc gggcgtccgg
    20221 cagaggaatc ttcttttcgc cgcggagata gtcgagcgtg accgagtcgg agggattggt
    20281 cttggcggta aggagcttct cgagccagcc cgc
//
LOCUS       DASZYS010000033        16126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1133901, whole genome shotgun sequence.
ACCESSION   DASZYS010000033 DASZYS010000000
VERSION     DASZYS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16126
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133901"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..442)
                     /locus_tag="VHF05_01980"
     CDS             complement(200..442)
                     /locus_tag="VHF05_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79724.1"
                     /translation="MSQRFPASSSEDVVWVPNSKEGRNVALVFFVMFAIAVEAFSIWL
                     CAYPATLTKAVPVIIVVLLAHVLCLLAITYKYGKYL"
     gene            complement(494..973)
                     /locus_tag="VHF05_01985"
     CDS             complement(494..973)
                     /locus_tag="VHF05_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79725.1"
                     /translation="MEKPVEGRYIRYAVIAIFAVLFVSGGFITYHFANRIVIDPASYA
                     HAGKIVKEKFPLSYASSTDEEIGRKLYGKGYLTAEQHLGLRSVGLSDDEIAHLVTEKE
                     NSPTEKALWYVGFLTLWAAVFAAALAIVSSAFPSAGQASFREEASKRGEAVESEPEE"
     gene            1080..1412
                     /locus_tag="VHF05_01990"
     CDS             1080..1412
                     /locus_tag="VHF05_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79726.1"
                     /translation="MPSNRRSNTTKRNARNDSQAPNTGNRGFRGFEGLSPQMRQAVAE
                     LQFGLGITTVEEAVRMCVAEAHLQMFTRYQGTAYYYKEKGDRKFKVYLPDLYAKTFPH
                     LPDDEASG"
     gene            1511..2836
                     /locus_tag="VHF05_01995"
     CDS             1511..2836
                     /locus_tag="VHF05_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79727.1"
                     /translation="MRRIAASAWILLCAGIFCPAQTVNKVQNWSYEISGPTSTGIANW
                     EDWANGGGVQVCNLSAPNIWNGSCVARFSFSAPPPDLSGMRQTIAYNQTTAKPIQFFA
                     FLQGKNIQNDSSDKNGATLNCHVYYMDRTMDYCPTTLQTKNVGTFPFRQIGFNTANTD
                     DFPNDRNADGSLKPVQSVEIIPMMGQIAGIANFDSTQAYEYVNAFKGGITIMFDDGFS
                     EQKQYCQTLIQNGVPCTWAIVVSYLGTPGYVSLANVKTEQSNGAEIVDHSWSHLPMAG
                     DSTHAPMNVNQLQHEFYDSKVWFVQNGITVNHFMLPYGSYNAQVIGEGGLDCWYNHNK
                     CFNSIRSTDRGSNPIGQFPWNIHIQEVDTHKDDGTLLTMGDVDNWIAEAKQNKRWLVI
                     LFHKVRSNCAGDNYCTSPSMFTSVMNAAKQSGLSLYTYSQGFNASEAMK"
     gene            complement(2919..3599)
                     /locus_tag="VHF05_02000"
     CDS             complement(2919..3599)
                     /locus_tag="VHF05_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVS79728.1"
                     /translation="MMEENVSFVTEDGVKIVGSYFEGVGERGALLLHMMPADRSSWHA
                     FAERLQAEGFDVLAIDLRGHGESVRTREGQTLDFRDFSGEETADAIHDVEAALRFLEK
                     KGIPLESIAICGASIGANLGIQAAADAPSIPALLLLSPGISYHGIESLPDAARLLNTQ
                     SVCIIAAKDDPNNKNVEAEAKEIFDALETEKQIKILDAGGHGTDMFEAHPELVDEAAA
                     WLAAAMLT"
     gene            complement(3634..5133)
                     /locus_tag="VHF05_02005"
     CDS             complement(3634..5133)
                     /locus_tag="VHF05_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVS79729.1"
                     /translation="METAAGDNAKMWKKGLIALVLLAVAALGIFLFRLAHEPLLDYDE
                     AAYAELVRETAASGNILALKFMGGNWFDKPPLFYWLAMAAEKLPVSAEFAMRLPSALA
                     SVAAVLLTYLIGLELTRSKRAAFISAAMLLTTAGFVEAGRQARPDALVLAFILLAFWG
                     FARGLMRENASRWFLLFGAGVGLAVMTKDAIGLLAYLFACAAALAFQKWDWLWSKYFW
                     LGQLLAAAIAAPWHAYMTAEFGASFWKTYILTHVFMRYETNLIGNNTSILTYVWYLAD
                     YASPWIVLFLVGIIALFAFRRFGGANRPAHLMLLLSALGVFALFSAASTKIPFYLLPM
                     LPFVALFAGMIAHEALSAFRPRWRKWFAGFLVACFAGAFALAIYRGYDLDPSFNIEHR
                     LAGDERSIGLILASDGTDLPIRFADPIYWETIRFYSRGRQLGSFDPAAAYGAPFLLVV
                     PTYKVNHYPYFETLAADSEIIFKGDYFTLMKIGGGSSGMPDSAQGSAGI"
     gene            complement(5212..5460)
                     /locus_tag="VHF05_02010"
     CDS             complement(5212..5460)
                     /locus_tag="VHF05_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVS79730.1"
                     /translation="MNFLLWVIFGGIAGWIASIIVGNNPQMGILANIVVGIIGALLGG
                     WIAHGVGARDAEHPATISGFIWAIIGAIVLLVILNLIF"
     gene            5583..6560
                     /locus_tag="VHF05_02015"
     CDS             5583..6560
                     /locus_tag="VHF05_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVS79731.1"
                     /translation="MKKRSGIATWALFVPLLAGSFYILLIEQRRPLLTYGGIVPRQTL
                     ITLFLIAAVLVALIVYYTFQNVQSIRIRQKQERLAVTAYKTLRVELQKKDDFISIASH
                     ELKTPLTTLKIYIQALQNFAAERGDSDYAPFLEKVDQQASKLNDLVENLLDIARIEGA
                     KMRYVMRPFDLDALMRACAEDSRIASEQHEIRISGQVGIPVYGDRDRIAQVITNLLTN
                     AVKYSPDMNVIDVSLALAGARARVDIRDYGIGIPRDKQAKIFEKFYRVTEGVSFPGMG
                     VGLYISAQIVAQHGGRLEVKSEPGNGSTFSFTLPLGSSFPTEPIRPPEP"
     gene            6715..7281
                     /locus_tag="VHF05_02020"
     CDS             6715..7281
                     /locus_tag="VHF05_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HVS79732.1"
                     /translation="MRNQPLPAALSEASTDLFDPLRTIHWMGGVSSGNYLDVLDEMRA
                     LLKEPLRAINLVVTSAGGPTGIAMSFYDSMRKIYRPHLRTIGSGDVDSSGIIVFLSGE
                     ERYISPNTTMLLHLAGRTFDPSRRYTVPDMEAMAKEDKLKDFQYASVIAENSGGRLDA
                     ERVLGMMAANTILTSLEAVELGLAHRVL"
     gene            7436..7615
                     /locus_tag="VHF05_02025"
                     /pseudo
     CDS             7436..7615
                     /locus_tag="VHF05_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7714..8094)
                     /locus_tag="VHF05_02030"
     CDS             complement(7714..8094)
                     /locus_tag="VHF05_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVS79733.1"
                     /translation="MNATQPKKVLIVDDDLGILEALKFMLENSGYEVKTVSRGDEALP
                     VLKEFGPDIVLLDLYLSGFDGRDIAVEIKNSPEGKNTPIAMISAHPSAGEVLKELDIE
                     EFLPKPFDVSQLLSVIEKHTSASR"
     gene            complement(8103..10763)
                     /locus_tag="VHF05_02035"
     CDS             complement(8103..10763)
                     /locus_tag="VHF05_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVS79734.1"
                     /translation="MKRKLGRNSHSTSAASERGARAKKSKKQTMRKSSGVVGAAPLRH
                     GRSTATMLAARMHRQEILALLSQIALATVDIDVSEFMQEACLFLARTLEADYAKVLQL
                     DPGGQTLTLRAGYGWNKSIMRVGATKFKSNSTIAGLTLTSKHPIIIKDLRREKKLRGF
                     AHLKAHHVTSGMSVIIHGIRGPYGVLGVYSRASRSYSADDLNFLQSVANIMALVFERS
                     DAENELRRFKYMADNANIGISLVDKNGRFHYVNRITYERLGFTKGEYMKKRVVHTPAG
                     SSQSQFRRLFQRAQKQQITQLETLEMRKDGVEIPLEVNLTGVSFEGEPYIFVVSQDIS
                     ERVRSQAEKLRSQRNLEFLSEASLLVGSSLDFQKNLGKVARKAVSYIADACAIEMADH
                     TGGFASAAIAHREPKKSRLWRASRDEFGGGEGLFGRAPHVAAAGRAELHSHVAPSMLR
                     KWSKSRSHFAALQRLDAASLMIVPVMIGKRVMGTMVFANSSGSRRFDDADLAIVTELA
                     NRVALAIENSKLYFEIEEQKERLDNTIANVPGIVWENWVNPDPATQRSNFISDYVEEM
                     TGYGERDWKRPGFMRDILHPEDRDRVLREYDEMVEKKRGMVQHFRLVHKNGGIIWVES
                     HSLAFKDARGRLLGFRGVIMDVSERTELERRKDEFISMASHELKTPVTTLKVFTQILQ
                     KKTENAGESITAAYLSKMDAQITRLTDLINDLLDLSKIQTGKLTFKYSDFKIDSLVGE
                     IVEQLQPTTDTHAINVIGRVGRLVHGDRDRIGQVLINLLTNAIKYSPKADKVNIYLSE
                     RGGNVIIDVEDFGIGISEEQQSRIFERFYQASEGSRTTFPGLGIGLYISDQIMKRHGG
                     KITVLSEKGKRTVFRVLLPVTAVHEKLTLV"
     gene            10973..11362
                     /locus_tag="VHF05_02040"
     CDS             10973..11362
                     /locus_tag="VHF05_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVS79735.1"
                     /translation="MQSDSSSEKRKVLICEDDPGILDAVSLALGSMGFEYDTHGMLEG
                     LEGCIEKVRPDVLLLDLWLGGANGEAILKELMENHPQTQIILMSAHSKLPMIAEKYGV
                     PYLPKPFGIDDLLRAVSAENNESPFQK"
     gene            11523..12752
                     /locus_tag="VHF05_02045"
     CDS             11523..12752
                     /locus_tag="VHF05_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVS79736.1"
                     /translation="MNTSGNAVEFGSRSGANPDERQDRPSKTAADPPHGRHKRESQSR
                     ERLLQAAVFSHLGEGMIVLDHEGKILLANGRARQLLGFSEKEKLHGIRYDRRFKLRTR
                     AGIALAQENHPVERALQNIEPIRISLMDDIYCVPKEDEAFPVTLTVSPIVFPHAKKIS
                     GVAITFYDITQEKKMDEIKSDFIYIASHQLRTPLTVSMLHTEMLLSGHGGELTKDQRE
                     FLNEIDFYNKKMAQLLTVFMTVSKIELGTFTLQEKPTDLRAILDDVFHELTTQIKSKN
                     LRIRQEYADEGGSVMTDPEFIRIAFQNLVSNAVKYTPHEGSITARIRRDADDVLIEVE
                     DSGCGIPEKEQSQVFSKLYRGSNTANHRWESNGLGLYLTKSIIDRCGGTIRFKSEENK
                     GTLFTISLPLKEEAREA"
     gene            12851..13366
                     /locus_tag="VHF05_02050"
     CDS             12851..13366
                     /locus_tag="VHF05_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79737.1"
                     /translation="MGRIPHALFVFAVLIAAGASSARAAHRKRIEAKVEIALIKTSLY
                     RNLPEAALTPTAHGLALARPLPPKREAELKALLNDRKNWKKDWTFFVRLWHHDCRLSV
                     RQKHAHPSLHVLGYADAEGRMYYSFHVDRYVADPWSEPLDTMKHVSKEVVPHFIRNRV
                     RPVGRLIAKLF"
     gene            complement(13579..15429)
                     /locus_tag="VHF05_02055"
     CDS             complement(13579..15429)
                     /locus_tag="VHF05_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HVS79738.1"
                     /translation="MSKFYRGIGIMAALVLLGIIAFGSYYIKIDWGKNQALARASSSV
                     SSPSYSFNFSGKSTIEEASKPGDSDSKDWWVESGGLLKLNNGVGETLEGNLPDDDSMR
                     KKYAKSDPTESDNGYHPQNIFRMFTQNEWQDSEQTMYMKVMGSNLFNPKNSNSFNGLS
                     LVARYQDDSDYYYLGVQMDGKAVIKKKKGGQFETLDSESLFSGKYDPAQNPNLIPDNK
                     LIGLRSRVINNSDGSVSLQLYVDTSGKGSWKLAAQAVDKGRDLGAPLTKAGNGGVITD
                     YMNVEFSDYEVSVLNAKDGGSDTAKDASSSNDSSDKSTDSSDSSSTDSGKNDSSSKDN
                     SSDSSSSSYDSAVLSDKPVMFLTMGNPGSGSETDKSGNGNDGKYKGGTPSTAKLPNGD
                     SAAVFNGSSQYLSVPSDASLSISTTHKLTWEAWIKPDSFNFSNASEDGYVDWMGKCAD
                     YSPTCEWEARLYGNDTAEGRPDRLSAYVFNSGAGLGSGADWQPSSKLQTGQWIHVVAE
                     YDEDSTPSPCSSSYPGTISIWVDGVKQDFGSHAPTGCMSQYKVKPKANDSPLNIGTMA
                     FDTWFKGAIGKVAVYDHLLSASEIADHYKSMTGSSPSGSCKDTCTAKGIM"
     gene            15965..>16126
                     /locus_tag="VHF05_02060"
     CDS             15965..>16126
                     /locus_tag="VHF05_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVS79739.1"
                     /translation="MTRNTKSVAVVGLGYVGLPLALLADRKGYKTYGIDIDANKIEKL
                     SKRIAPYKDI"
BASE COUNT         3779 a         4552 c         4326 g         3469 t
ORIGIN      
        1 caggaggcag tcatggaacc gagcttgaaa cgcctgatcc tcttgctatt cctttttctt
       61 ttcgcaaccg cttcatgcgg ctcgcgtccg cattatcggc atcgccatcc tccgccgagg
      121 acgcggcggc atcgcagaca ccgccgcaat agacacacag ggcgcctcct ttggaggcga
      181 ccttttttat cttgaatttc tagaggtact tgccgtactt atacgtgatc gccaggaggc
      241 agagtacatg agcgagaagc acgacgatga tgaccggcac ggccttggtg agcgttgccg
      301 gatacgcgca gagccagatg gagaatgcct ccaccgcaat cgcgaacatg acgaaaaaca
      361 cgagggcgac attccggccc tctttcgaat taggaaccca tacgacatct tccgaactgc
      421 ttgcgggaaa acgttgagac atgcgctttt attataaact aaagccccgg cggagcgaat
      481 acacacccga aggtcattct tcgggttcgc tctcgaccgc ttcgccccgc ttgctcgcct
      541 cttcccggaa ggacgcctgc cccgccgaag gaaaagccga cgaaacgatc gcgagagcgg
      601 cggcaaagac ggcagcccag agcgtcagaa aaccgacata ccagagcgct ttctctgtgg
      661 gagaattttc tttctctgtc acgagatgcg caatctcatc atcgctcaag ccgacgctcc
      721 tcaaccccag gtgctgttcg gcggtgagat atcccttgcc gtagagcttc cttccgattt
      781 cctcgtcggt cgagctcgca taggacaaag ggaatttttc cttcacgatc ttgcctgcat
      841 gggcgtagct tgccggatcg atgacgatcc ggttcgcgaa gtgataggtg atgaagccgc
      901 ccgacacgaa aagcaccgcg aaaatcgcga tcacggcgta gcgaatatat cgcccctcca
      961 caggcttttc catagggaat attatacatc ccccgcttca gaactttgaa gcgcgctgtg
     1021 gacttcatcg atatgacata atcggcatgg tatgaatatc accggaagga gatgcgcaca
     1081 tgccatcaaa ccgaaggagc aatacaacca agagaaatgc gagaaacgat tcgcaggcgc
     1141 cgaacaccgg caatcggggg ttcagggggt ttgaaggtct ctcgcctcaa atgcgccagg
     1201 ccgttgccga actccagttc ggcttgggaa taacgacagt ggaggaagcg gtacggatgt
     1261 gcgtcgccga ggcgcacctt caaatgttta cccgatacca aggaaccgcc tattactaca
     1321 aggaaaaggg cgaccgcaag ttcaaggtgt atcttcctga tttatatgcg aaaacgttcc
     1381 cccacctccc cgatgacgaa gcgtccggat aatccggacg ttttcttttt ttccttcacc
     1441 tcgcattcac gcgaaagctg ttcaataaag cgggatagta cattacacat ggaaggaaga
     1501 gtaagagaga atgagacgaa ttgcggcttc tgcctggatt cttttatgcg cggggatatt
     1561 ttgcccggcg caaaccgtca acaaagtaca gaactggagt tatgagatat cagggccgac
     1621 ctcgaccgga atcgccaact gggaagactg ggcgaatggc ggaggggtgc aggtatgcaa
     1681 tctctccgcg ccgaatatct ggaacggatc atgcgtcgcg cgattcagct tcagcgcgcc
     1741 tccgcccgat ctgtcgggca tgcggcagac aatcgcctac aatcagacga ccgcaaagcc
     1801 catacaattc tttgcgttcc tccagggaaa gaacatccaa aacgattcga gcgacaagaa
     1861 cggggcaacg ctcaattgcc acgtctatta catggatcgc accatggatt actgcccgac
     1921 cacgcttcaa accaagaacg tcggcacgtt tccgttccgc cagataggct tcaacaccgc
     1981 gaataccgac gactttccga acgacaggaa tgccgacggg agcctcaaac cagtgcaatc
     2041 cgttgagatc attcccatga tggggcagat cgctggcatc gcgaatttcg actcgacgca
     2101 agcttacgaa tatgtaaacg cattcaaggg aggaatcacc atcatgttcg atgacggctt
     2161 cagcgagcag aagcagtatt gccaaacgct cattcaaaac ggagttccct gcacatgggc
     2221 gatcgtggtg agctatctcg gcactcccgg atatgtctcg ctcgccaacg taaaaacaga
     2281 gcaatcgaat ggggcggaaa tcgtcgacca cagctggtcg cacctgccga tggccggcga
     2341 cagcactcat gcgccgatga acgtgaacca gcttcagcac gaattttacg acagcaaagt
     2401 atggttcgtg caaaacggca ttaccgtgaa tcatttcatg ctcccttacg gctcatacaa
     2461 tgctcaggtg atcggagaag gaggcctcga ctgctggtac aaccacaaca aatgcttcaa
     2521 ttccatccgc tcgaccgatc gcggatcgaa tccgatcggc caattcccct ggaacatcca
     2581 catccaggaa gtcgatacgc acaaagacga cggtacgctc ctcaccatgg gagacgtcga
     2641 caattggatt gccgaagcca agcagaacaa gcgctggctc gtaatcctct tccacaaagt
     2701 ccgaagcaac tgcgcgggcg acaattactg cacgtcgcct tccatgttca cttcagtcat
     2761 gaacgccgca aagcagagcg gcctgagcct ttatacgtac agccaaggct tcaacgcctc
     2821 ggaggccatg aaataactcg ggaagaaaag cagcatgcaa atgcccgtgg aacgacgcga
     2881 agtcgtccca cgggattttt aaatccgaca tgagaaaatt acgtcagcat tgccgccgcg
     2941 agccatgcgg ccgcttcgtc gacaagctcc gggtgggctt cgaacatgtc cgtaccgtgt
     3001 ccgcctgcat ctaaaatctt gatctgcttc tccgtttcaa gcgcatcgaa aatctccttc
     3061 gcttcggctt ccacgttttt gttgttcgga tcgtccttgg ccgcaatgat gcagaccgat
     3121 tgggtgttca agagacgcgc ggcgtccgga agcgattcga tgccgtgata actgatgccg
     3181 ggagaaagga gaagcagtgc ggggatcgag ggggcgtccg cggccgcctg tatgccgaga
     3241 ttcgcgccga tagaggctcc gcagatggca atgctctcca agggtattcc cttcttttcc
     3301 agaaagcgga gagcggcttc gacatcgtga atcgcgtcag cggtctcctc gccggagaaa
     3361 tcgcggaagt cgagggtctg cccctcgcgg gtccgcaccg actcgccgtg gccgcgaaga
     3421 tcgatcgcca atacgtcgaa cccttcagct tgaaggcgct cggcgaacgc atgccagctc
     3481 gagcggtcgg cgggcatcat gtgaaggagg agcgcgccgc gctctcccac gccctcgaaa
     3541 tacgatccga cgatctttac tccgtcttcc gttacaaacg agacattttc ttccatcatg
     3601 ggatttttat acagaaagat tatgaaaaaa caattatatc ccggccgagc cttgagcgct
     3661 atccggcatg ccggagcttc cgccgcctat tttcatcagg gtgaaataat cccctttgaa
     3721 gatgatctcc gaatccgcgg caagcgtttc gaaatacgga taatgattga ccttgtatgt
     3781 cgggacaaca agaaggaacg gcgcgccgta ggcggcggcc ggatcgaagc tcccgagctg
     3841 gcggccgcga ctgtaaaagc ggatcgtctc ccaatagatc ggatctgcaa accttatcgg
     3901 aaggtcagtg ccgtcgctcg cgagaatgag gccgatcgag cgctcgtctc ccgcaaggcg
     3961 atgctcgata ttgaaagagg gatcgagatc gtagccgcga tatatcgcca gcgcgaaggc
     4021 gcctgcgaaa caagcaacga gaaatcctgc gaaccatttt ctccagcgag gccggaaggc
     4081 gctcaaagcc tcgtgcgcga tcatgcctgc gaagagcgcg acgaacggaa gcatcggcag
     4141 aaggtagaac gggattttgg tcgatgcggc cgagaagagc gcgaacactc ccagcgccga
     4201 gagcaaaagc atgagatggg cggggcgatt cgcgccgcca aagcgccgaa aagcgaaaag
     4261 cgcgattatg ccgacgagaa aaagaacgat ccagggcgag gcgtaatccg caagatacca
     4321 tacataagtg aggatcgacg tgttgttgcc gatgagattc gtttcatagc gcatgaaaac
     4381 gtgagtgaga atgtacgttt tccagaatga ggcgccgaac tccgcggtca tataggcatg
     4441 ccagggcgcc gcgatcgccg cggcaagaag ctggccgagc cagaagtatt tgctccaaag
     4501 ccaatcccat ttctgaaacg ccaaagcggc cgcgcaagca aagagatacg caagaaggcc
     4561 tatggcgtct tttgtcatga ccgcgagccc gactccggcg ccgaacagca gaaaccagcg
     4621 ggatgcgttt tcgcgcataa gcccgcgcgc gaagccccag aacgcaagaa ggatgaatgc
     4681 gaggacaagc gcatcgggcc gcgcttgccg ccccgcctcg acgaagcccg cggtcgtgag
     4741 aagcatcgcg gcggagatga atgcggctcg cttacttcga gtgagctcga ggccgatgag
     4801 atacgtaagg agaacggcgg cgacggaggc gagcgcgctt gggagcctca tggcaaattc
     4861 ggcggagacc ggaagctttt cggccgccat ggcaagccag taaaacaagg gcggcttgtc
     4921 gaaccagttc cctcccatga acttgagtgc gagaatgttc ccgctcgcgg cggtttcgcg
     4981 cacgagctcc gcgtatgccg cctcgtcata gtcgaggagc ggctcatggg ccaggcggaa
     5041 cagaaaaatc ccgagagcgg caacggcaag gagcacgaga gcgatcaaac ccttcttcca
     5101 catcttcgcg ttgtcgcctg cggctgtttc catgctcaaa atgatagcaa aaggagtctt
     5161 gcgcgaaaaa cgcggcctct cggccgcgcc ttgcgcttca tgacccgcca gctagaagat
     5221 aaggttgaga atcaccagca gaacgatcgc tccgatgata gcccaaatga atccggaaat
     5281 agtggcggga tgctcggcgt cgcgggcgcc caccccatgc gcgatccagc cgccaagcaa
     5341 ggcgccgatt atgccgacaa cgatattcgc gagaatgccc atctgcggat tatttcctac
     5401 gatgatgctc gcgatccagc ctgcgatgcc tccgaaaatt acccagagca aaaaattcat
     5461 aaaaattgaa ttgttatggt aatgcatcaa ttatcactga tgagcaatga aaaaagcatg
     5521 aacgacatga aaaagctgcg gacgatcttg cgccgttttc tggccggtgt tttaattaga
     5581 ccatgaaaaa gaggtcaggc attgcaacct gggccctctt tgttcctctt ctggctggaa
     5641 gcttctacat tctcctcatc gaacagaggc ggcccttgct cacatatggg ggaattgtcc
     5701 cgaggcaaac tctcatcaca ctttttctca ttgccgccgt cctggtggca ttgattgtct
     5761 attacacctt ccagaacgtc caaagcatcc gcatccggca gaagcaggag cggctcgccg
     5821 tgaccgctta caagacgctt cgggtggaac tgcagaaaaa agacgatttc atctcgatcg
     5881 cgtcgcacga gctcaagact cccctgacga ccctgaagat ctacatccaa gcgctccaga
     5941 acttcgccgc cgagcgaggc gacagcgact acgcgccctt tctggagaaa gtggaccagc
     6001 aggcatcgaa attgaacgac ctcgtcgaga accttctcga catcgcccgc atcgaaggag
     6061 ccaagatgag gtacgtcatg cggcccttcg atctggatgc cctgatgcgg gcatgcgccg
     6121 aagactcgcg catcgcgagc gaacagcatg agatcaggat atccggccag gtgggaattc
     6181 ccgtatacgg cgaccgcgac cgcatcgcgc aggtcattac gaacctgctt accaatgcgg
     6241 tcaaatactc gcccgatatg aacgtcatcg atgtcagcct cgctctggcc ggcgcccggg
     6301 cgcgcgtcga tatccgcgac tacggcatcg gcattccccg cgacaagcag gcgaaaatat
     6361 tcgagaaatt ctaccgcgta acggaaggcg tttcgtttcc gggcatgggc gtcggactct
     6421 atatttccgc gcagatcgtc gcccagcatg gcggccggct cgaagtgaag agcgagccgg
     6481 gaaatggatc gacgttctcc tttacgctgc cgctgggaag ctccttcccc accgaaccga
     6541 tccgtccgcc ggagccttag gcggtgttcg tatatatcgc ggtacccgcg ggagaatata
     6601 tgtgaacact gcccaattac gacacactgc cgagcttgtg gcggcacgct ccggaaaata
     6661 taatttatga atatttcgtg aggaattagg aatttagcaa ttaacgatat attcatgcgc
     6721 aaccagcccc ttcccgccgc tttaagcgag gcgtcgacgg atttattcga cccattgagg
     6781 acaattcatt ggatgggagg ggtttcaagc ggcaattatc ttgatgtctt agacgaaatg
     6841 cgcgctctct taaaggagcc tttgcgcgcc ataaacctcg tcgtcacctc ggcaggcggc
     6901 cctacaggca tcgccatgag cttttacgac tccatgcgaa agatctaccg cccccatctc
     6961 cgaaccatcg gttcgggcga cgtggactcc tcgggcatca tcgtttttct ttccggcgaa
     7021 gagcgctata tctcgcccaa taccacgatg ctccttcatc ttgcgggcag gacgttcgac
     7081 ccgtcgcgcc gctatacggt gcccgatatg gaggcgatgg cgaaggaaga taaactgaag
     7141 gatttccagt acgcgagcgt catcgccgaa aattccggcg gccggctcga tgcggagcgc
     7201 gtgctcggca tgatggccgc gaacaccatc ctcacgtcgc tcgaagcggt tgagctcggc
     7261 cttgcacatc gggtgctcta agaagaagtg ggcagtttct agcttgctga aagtcagctt
     7321 cggatttttt cccgcagcca taggctctta cgtttttcat atgcttttca tcaccttcct
     7381 gtagagttgg ggtggtcgaa gccatcatac tcttcatttc attcacatcc attctatgca
     7441 acgcaaacaa cgcagaggag ctgcgaaaag caacaggggc ttccgggcaa tgaaagacaa
     7501 gaagcgccag cgtgagatag cgagcgccgg cggcaaagcc gctcatgcga aaggtacggc
     7561 ccacaagttc acctcggcgg aagcgaagcg tgcgggccgc aaaggcggca gggcgcgcag
     7621 cagaaaagcc gctcgcggca gaaggtagaa aaaagaagaa gccccgtgag atagcgcgaa
     7681 gcgcggcatc tcacggggct ttttcgcgta tcttcagcgg gaagcggacg tgtgtttctc
     7741 gatgacggag agaagctggc tcacgtcgaa aggctttggc aggaattcct cgatatcgag
     7801 ctccttcagg acttctcccg cgctcgggtg cgccgaaatc atcgcaatgg gggtgttttt
     7861 gccttcgggc gaattcttga tctcgaccgc gatatccctc ccgtcgaatc cggaaagata
     7921 gagatcgagc agaacgatat ccggcccgaa ttccttgagc actggaagcg cctcatcccc
     7981 ccgagagacc gttttcactt catacccgga gttttcaagc atgaatttca atgcctcgag
     8041 gattccgagg tcgtcatcta cgataaggac tttcttgggt tgtgtcgcgt tcatggtgtg
     8101 gattatacca atgttagctt ttcatgcact gcagtaacgg gcaagaggac cctgaagacg
     8161 gttcgcttgc ctttttcgct caagaccgtg attttgcctc cgtggcgctt catgatctgg
     8221 tcggaaatat agagaccaat gccgaggccg gggaacgtcg tccgcgatcc ttcgctagcc
     8281 tgatagaacc gctcgaagat cctcgactgc tgttcctcgg agatgccgat gccgaaatcc
     8341 tcgacatcga tgatcacatt gcctccgcgc tccgaaagat aaatattcac cttgtcggcc
     8401 ttcggcgaat atttgatagc gttcgtaagc agattgatga gcacttggcc gatccgatcg
     8461 cgatcgccgt gaacgagcct tccgacacga cctataacat tgatcgcatg cgtgtccgta
     8521 gtcggctgga gctgctcgac gatctccccc accagactgt cgatcttgaa atctgaatac
     8581 ttgaacgtga gcttgccggt ttggatcttg gaaaggtcga gtaggtcgtt gatgagatcg
     8641 gtaaggcgcg tgatttgcgc gtccatcttc gaaagatagg cggcagtgat actctcgccg
     8701 gcgttctcgg tttttttctg gagaatctgg gtgaaaacct tcaatgtcgt taccggcgtc
     8761 ttgagctcgt gcgacgccat gctgatgaat tcatccttcc tgcgttcgag ctccgtgcgc
     8821 tccgacacgt ccatgatgac gccgcgaaac cccaggagcc tcccgcgcgc gtctttgaaa
     8881 gccaaactgt gcgattcgac ccagatgatg ccgccgttct tgtgcacgag ccggaaatgc
     8941 tggaccatgc cgcgcttttt ttcgaccatc tcatcatatt cgcgaagcac gcgatcgcga
     9001 tcctccggat gcaggatatc tcgcatgaag ccgggccgct tccagtcgcg ttcgccgtag
     9061 ccggtcatct cttcgacata gtcgctgata aaattactcc tctgcgtcgc cggatcggga
     9121 ttcacccagt tctcccatac gatgcccgga acgttcgcaa tggtgttgtc gagccgttcc
     9181 ttctgctctt ctatttcgaa ataaagcttc gagttctcga tcgccagtgc tacgcgattc
     9241 gcaagctcgg tcacgatcgc aagatccgca tcatcgaatc tgcggctgcc ggaagaattc
     9301 gcgaacacca tcgttcccat cacccgcttg ccaatcatca cgggaacaat catgagcgaa
     9361 gcggcatcga gccgttggag cgccgcgaaa tggctcctgc tcttcgacca cttgcggagc
     9421 atggaaggag caacatgcga atggagctca gccctcccag ccgcagcaac atgcggcgcc
     9481 ctgccgaaga gaccttctcc gccgccgaat tcgtcgcggc tcgctcgcca tagacgactt
     9541 tttttcggct cgcgatgagc gatcgcggcg gatgcgaagc cgccggtgtg atccgccatt
     9601 tcaatggcgc atgcatccgc tatgtacgag acggctttcc ttgcaacctt tcccagattc
     9661 ttctggaaat cgagcgatga tcccacaaga aggctcgctt cggagaggaa ttccagattg
     9721 cgctgagagc ggagcttctc cgcctgcgac cgcacgcgtt cgcttatatc ctgcgatacg
     9781 acaaaaatat acggctcccc ttcgaacgat actcccgtaa gattgacctc gaggggaatt
     9841 tccacgccat ccttcctcat ctcgagcgtt tcgagctggg taatctgctg cttctgcgcc
     9901 cgttggaaaa gcctcctgaa ttgagactgg ctgctgcctg ccggcgtatg cacgacccgt
     9961 tttttcatgt actctccctt cgtaaagccc aaccgctcgt aggtgattcg attcacataa
    10021 tgaaaccggc cgtttttgtc gacgagcgaa atgccgatgt tcgcgttatc agccatatat
    10081 ttgaaccggc gcagttcgtt ttccgcatca ctgcgctcga agacgagcgc catgatattg
    10141 gcgaccgatt gcaggaagtt cagatcgtcc gccgaataag atcgagaggc gcggctatac
    10201 acgccgagca cgccatacgg ccccctgatc ccgtgaatga tgacgctcat gccgcttgta
    10261 acatgatggg ctttcagatg cgcgaatcca cggagttttt tctcccgccg caaatccttg
    10321 ataatgatcg ggtgcttcga agtgagtgtg aggcccgcaa tggtcgaatt cgatttgaat
    10381 ttggtcgcgc cgacccgcat gatgctcttg ttccatccgt agcccgcccg aagagtgaga
    10441 gtctgtccgc ccgggtcgag ctgaaggact ttcgcataat ccgcttccaa ggtgcgcgcg
    10501 agaaagaggc atgcctcctg catgaattcg ctcacgtcga tatcgacagt ggccagggca
    10561 atctgggaaa gaagggccag gatctcctga cgatgcatgc gcgccgcaag catcgtggcg
    10621 gtggagcggc catgacggag aggtgcggcg ccaacgacgc ccgacgactt tcgcatcgtc
    10681 tgtttctttg attttttggc ccgagcgccg cgctccgatg cggcggaagt cgaatgtgaa
    10741 ttgcgcccga gttttctttt catagcaaaa aatgattatc gggagaaaag atgaattttc
    10801 aatgaaaatt aaaataccac actttctgcg caatatcaaa gcatagcatc ccatggacag
    10861 agcggatcgg caggattttc aggcgttcgg caagaaagcc cacggcagag cattcgtcta
    10921 tatagatacg accagtcaat tgagcggccg cgcgccgtag aacggtcgaa atatgcaatc
    10981 cgattcctcc tcggaaaaaa gaaaagtgct catctgcgaa gacgaccccg gcatcctcga
    11041 tgctgtcagc cttgcgcttg gatcgatggg cttcgagtac gatacccacg gcatgctcga
    11101 agggctcgaa ggctgcatcg agaaagtgcg ccccgacgtc cttcttctcg acctctggct
    11161 cggcggcgcg aacggcgaag caattctcaa agaactcatg gaaaaccatc ctcaaacgca
    11221 gatcattctc atgtcggcgc atagcaagct tccgatgatc gccgaaaaat acggcgtccc
    11281 ctaccttccg aagccgttcg gcatcgacga tcttcttcgc gcagtctctg cagaaaacaa
    11341 cgagagcccg tttcaaaaat aactggacgg atttttgaga aaatccgagc gcttcatgga
    11401 aaattcatgg agaaaatcta gtatattctg catacagctc cattcgcgca gctagctcgg
    11461 cattgggagt aggcagagag gacgtctgcg cgcattttgt tcggtaacat tttcaccact
    11521 caatgaatac aagcggcaac gcggtcgaat tcgggtctcg aagcggcgcg aacccggacg
    11581 aaagacagga tcgcccttcg aaaactgctg ccgaccctcc gcacggccgg cacaagagag
    11641 aatcgcagag cagggagagg cttttgcaag cggccgtctt ttcccatctc ggggagggca
    11701 tgatcgttct cgaccatgaa ggaaaaatcc tcctggcaaa cggcagggcc cgccagctcc
    11761 tcggcttcag cgaaaaagaa aagcttcacg gcatacgcta cgatcgccgt ttcaagctcc
    11821 gaacgcgcgc aggcatagcc ctagcgcagg aaaatcatcc tgtcgagcgc gctttacaaa
    11881 atatcgagcc gatccggatt tccctcatgg acgacatata ttgcgttccg aaggaggatg
    11941 aggcgtttcc ggtgacgctt acggtttccc ccatcgtttt tcctcatgcc aaaaagattt
    12001 cgggagtggc gatcactttc tatgacatca cccaggagaa gaaaatggac gagatcaagt
    12061 ccgatttcat ctacatcgcg tcgcaccagc tccgcactcc cctcaccgtt tcgatgctcc
    12121 atacggagat gctcctttcc ggccacggag gcgaactcac gaaggatcag cgcgaatttt
    12181 tgaacgagat cgatttctac aacaagaaaa tggcccagct cctcactgtg ttcatgacgg
    12241 tgtcgaagat cgagctcggc accttcacgc tccaggagaa gccgaccgac ctgcgcgcca
    12301 ttttggatga cgtgttccat gaacttacga cgcagataaa gagcaagaat ctccgcatac
    12361 ggcaggaata tgcggacgaa gggggcagcg tgatgaccga tccggaattc atccgcatcg
    12421 cgttccaaaa tctcgtatcg aacgcggtga aatacactcc gcacgaagga agcataactg
    12481 caagaatcag gagagacgcg gacgatgttc tgatcgaagt ggaagacagc ggctgcggaa
    12541 tccccgagaa ggagcagtcg caggtcttct cgaagctcta ccgcggctcg aacaccgcga
    12601 accaccgatg ggaatcgaac ggcctcggcc tttacctcac caagtcgatc atcgacaggt
    12661 gcggcggcac gatccggttc aagtccgagg agaacaaggg tacgcttttc actatttccc
    12721 ttcccctaaa ggaggaggcg cgggaagcgt aggcgcggca gtattgcagt ttccggccat
    12781 cggtgccata gtaacagcgg atttgcgaac gaaaaataag gaatttggag aaaggaggcc
    12841 tattcccatc atgggccgca taccgcacgc gctatttgtt tttgccgttc ttatagcggc
    12901 gggagcttcg tccgcccgcg cggcgcacag aaagcgcatc gaagcgaaag tcgaaatcgc
    12961 tctcatcaaa acgagcctct accgcaatct tcccgaagcg gcattgactc ctaccgcgca
    13021 cggattggct ctcgcgaggc cgctcccgcc aaaacgggaa gcggaactca aagcgctcct
    13081 caacgaccgc aaaaattgga agaaggattg gacgtttttc gtgcggctct ggcatcatga
    13141 ttgccgtctg agcgtccggc aaaagcacgc ccatccttcg ctccacgtcc tcggatatgc
    13201 ggacgcggag gggcgcatgt attattcatt ccatgtcgac cggtacgtgg ccgacccttg
    13261 gtcggaaccg ctcgatacca tgaagcacgt ctcgaaggaa gtcgttccgc acttcatcag
    13321 gaaccgcgtc cgccccgtcg gacgccttat cgcaaaattg ttctgaacgc gggctcccgc
    13381 gagaatgatc cgccgcaagc ggacgatctc acgggagctt ttttatggtg gaaaccttgc
    13441 gatatatcgc aaggtttcca ttacttatat ttataacgca tgcgagcctc tgggtctgaa
    13501 ttgcaataat aaggaaaacc gccgcacaga gtgcggcggc attcactata agtagtgcca
    13561 acatattttt tgttaaagct acatgattcc tttcgcggtg cacgtatcct tgcaggatcc
    13621 tgaaggactt gatccggtca tcgacttgta gtgatccgcg atctcgcttg cgctgagcaa
    13681 atgatcgtag acggccacct tgccgatcgc gcccttaaac cacgtgtcaa aggccatcgt
    13741 accgatattc agaggcgaat cgttcgcctt gggcttcact ttgtactggc tcatgcagcc
    13801 tgtcggcgca tgcgatccga agtcctgctt cacgccgtcg acccagatgc ttatggtgcc
    13861 agggtatgaa gagctgcagg gcgacggagt gctgtcctca tcgtactccg cgacgacgtg
    13921 aatccactgt cccgtctgga gcttcgagct cggctgccag tcggcgccgc tgcccaagcc
    13981 cgcgccggaa ttgaagacgt atgccgagag cctgtcgggg cggccctccg cggtgtcatt
    14041 gccgtaaagc ctggcttccc attcgcaggt cgggctgtaa tccgcgcact tgcccatcca
    14101 atcgacgtat ccatcctccg atgcgttcga gaaattgaac gagtcgggct tgatccacgc
    14161 ttcccatgtc agcttgtgcg tcgtcgagat cgagagcgac gcgtcggacg ggacgctgag
    14221 atattgcgag gagccgttga agacggcggc gctatcgccg ttcggaagct tcgcagtcga
    14281 aggcgtaccg cccttgtatt tgccgtcatt gccgtttccc gacttgtcgg tttcggagcc
    14341 cgatccgggg ttgcccatcg tgaggaacat taccggcttg tcggagagaa ccgccgagtc
    14401 gtacgaagac gacgaggaat cggatgaatt gtccttgctg ctcgaatcat tctttccgga
    14461 gtcggtggac gaactgtcgg agctgtcggt gctcttgtcg ctgctgtcat tactgcttga
    14521 cgcatccttg gccgtgtccg atccgccgtc tttcgcattg agaacgctta cctcgtaatc
    14581 cgagaactca acgttcatgt aatcggtaat gactcccccg ttgcctgctt tcgtaagagg
    14641 cgcaccaaga tcccttccct tgtccactgc ctgggccgcg agcttccaag aacccttgcc
    14701 cgacgtgtcg acatagagct gaagcgagac gctgccgtcg gagttgttga tcacccgcga
    14761 gcggagcccg atcagcttgt tgtccggaat gagattcggg ttctgtgcgg gatcgtactt
    14821 gccggagaac agggattccg aatcgagcgt ttcgaactgg ccgcctttct tcttcttgat
    14881 caccgccttg ccgtccatct ggacgccgag atagtagtaa tcgctgtcat cctgatatct
    14941 cgcgacgagc gaaagcccgt tgaaggaatt gctgttcttc ggattgaaga ggttgcttcc
    15001 catcactttc atgtacatcg tctgttcgga atcctgccac tcgttctgcg taaacatcct
    15061 gaaaatattt tgcgggtggt atccgttgtc gctttccgtc gggtcgctct tcgcgtactt
    15121 cttcctcatc gaatcgtcgt cgggaagatt cccttcgagg gtttctccga cgccgttatt
    15181 gagcttcaga agcccgcctg attcgaccca ccaatccttg ctgtccgagt cccccggctt
    15241 cgacgcctct tcgatcgtcg acttgccgct gaaattgaat gagtatgagg gagacgagac
    15301 cgagcttgag gcgcgcgcca gagcctgatt tttgccccaa tcgatcttaa tgtaatacga
    15361 accgaatgcg ataatgccga gaagcacgag cgccgccata atgccgatgc ctctgtaaaa
    15421 tttgctcatc actaaaagtt cccgatgcct aaatttctaa ctccagactg atgtccggga
    15481 atcgagagcg gggatccgtt ccgtcatctc acatcagtga aaagtatttt atttgattca
    15541 tcttaccttc acaattcggt tttcccgtcc gggcttcatg aacttgcatt ttgcccttac
    15601 agaggagttt ttttgacgag ttcaatcctt tattttcccc ttttgcggct caatacggcg
    15661 ctcattcacg tttaggttcc aaaatgtgcc tattcttaag cgttttttca ggatgcaaac
    15721 ctctgcagta cgacctttca aagggcataa aacgaagcaa cggctgtgca atttgggagg
    15781 tgcgaattat ttctcccctg cctgaattgc atccaaaaat ttcccgcgcg gaaaaggcgc
    15841 gggaccgaat gaagcgcgaa tgaattttct ttttgcgaat tcatgcaaac atcaccgccg
    15901 gaatgttatt catagggagt acaacaaaac ggccgaccgg ccgccaataa gttacgaaaa
    15961 atttatgaca cggaatacga aatcagtcgc ggtagtcgga cttggctacg tcggcctccc
    16021 tcttgcgctc cttgcggaca ggaagggcta caagacatac ggcatcgata tcgacgcaaa
    16081 caaaatcgag aagctgtcga agcgcatcgc gccctataag gacatc
//
LOCUS       DASZYS010000034         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_101988, whole genome shotgun sequence.
ACCESSION   DASZYS010000034 DASZYS010000000
VERSION     DASZYS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101988"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VHF05_02065"
     CDS             <1..546
                     /locus_tag="VHF05_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVS79740.1"
                     /translation="AFEPEDNETPLSSFGKSKGDALAELKEKLKASEAKAAEYLTDLQ
                     RMKAEFVNLRKRDEDEKKELVKYSNEKLILELLPVLDSFEMAFANKEAWEKADKNWRV
                     GVEYIHSQLVGIFEKHGVRTIDPLGEKFDPARDTPVEMVSTENESDDHKIIAVMQKGY
                     SLNGKEIRSPKVKVAEYKPAA"
     gene            674..982
                     /locus_tag="VHF05_02070"
     CDS             674..982
                     /locus_tag="VHF05_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79741.1"
                     /translation="MEIRPLHQKFVEEVWRDAQGRLARVTFFVYEHEGRMKARVVKVV
                     YLEEERFEKKDIPALAGNVAGSKPEADISLGESDIVSPYFDTEFLYTIGSKPRAPTFA
                     "
     gene            1133..3058
                     /gene="dnaK"
                     /locus_tag="VHF05_02075"
     CDS             1133..3058
                     /gene="dnaK"
                     /locus_tag="VHF05_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HVS79742.1"
                     /translation="MAKIIGIDLGTTNSAVAVMEVGEPKIIENAEGARTTPSIVAESK
                     TGERIVGLLAKRQAVTNPKNTIFQIKRFIGHTFDDPGVQKDIKSVPFEVRKSANGGVE
                     VKMGEQWYRPEEISAMILQKLKADAEARLGEKITEAVITVPAYFNDSQRQATKDAGKI
                     AGLEVKRIINEPTAAALAYGFNKKKEEKIAVFDFGGGTFDISVLEVGDDVIEVKSTDG
                     DAHLGGKDIDQKIINWLAEEFKKENGVDLMSDPLAVQRLDDAAEKAKIELSTAVQTEV
                     NIPFISSGPQGPMHLVKTMTRAKLEELTGEFVERAMSITKRAIEASPYKVGEINEVIL
                     VGGQTRMPAIQQAVKAFFGKEPHMGVNPDEVVALGAAIQGGILGGEVKDVLLLDVIPL
                     SLGIETMGGVATKLIERNTTIPSSRSQIFSTAADSQTSVEIHVVQGERAMASDNKSLG
                     KFMLDGIPPAPRGVPQVEVTFDVDANGILNVKAKEKTTGKEQSIRIEGSSGLSKEDIE
                     RMQKDAEMHGDEDKKKKEEAEAKNLAEQMIYAAEKSLKDFGDKVSEDIKKGVQEKIDA
                     LKKEKDGGNIEAIKTATDSLSAEMQKIGQAMNQNQTNNPEQKTADNQTGTQENVRDAD
                     FEEKKEGENPEGGQQKQ"
BASE COUNT          864 a          802 c          923 g          568 t
ORIGIN      
        1 gctttcgaac ccgaagacaa cgaaaccccg ctgagctctt tcggcaagtc gaaaggcgat
       61 gcccttgcgg agctcaaaga gaagctcaag gcgagcgagg cgaaagccgc ggaatatctc
      121 accgatctcc agcgcatgaa ggccgaattc gtgaacctcc gcaagcgcga cgaagatgag
      181 aagaaggagc ttgtgaaata ctcgaatgag aagctcattc tggagctcct gccggttctc
      241 gacagcttcg agatggcctt cgcgaacaag gaagcgtggg aaaaggctga caagaattgg
      301 cgcgtcggtg tggaatatat ccactcacag ctcgtgggaa tattcgagaa gcacggggtt
      361 cggacgatcg acccgttagg cgagaaattc gatcccgctc gcgacactcc cgtagaaatg
      421 gtttcgaccg aaaacgaaag cgatgaccac aagatcattg cagtcatgca gaagggctat
      481 tctttgaacg gcaaggaaat caggtcgccc aaggtgaaag tagccgagta caagcctgcg
      541 gcttaatttt caattttgaa ttctcaattt tcaatcaaat tgcaatctgg caatgtgcca
      601 atgcgcaatg gtttgaaaat tgagatcttg aaaattcatt gaaaattgga aattgaaaac
      661 ttataatttg cgtatggaga tacggccttt gcatcaaaaa ttcgtagaag aagtatggcg
      721 cgacgcgcag ggacgcttgg cgcgggtgac tttcttcgta tatgagcacg agggcaggat
      781 gaaggcccgc gtggtgaagg ttgtctatct tgaggaggag cgttttgaga agaaagacat
      841 tcccgcgctt gcgggaaacg tggcgggaag caagcctgag gcagacatct ctctcggcga
      901 atccgatata gtttctccgt attttgatac tgaatttctc tacacgatag gctccaagcc
      961 gcgcgctcca acttttgcat agttgattgt catcccgcgc tagcgggatc cagtcattcc
     1021 actgtatgcg gtttcacctc gttcaaggat catagaaaca acgtggttac cactggatgc
     1081 cctgctgggc atgacagata aggagagtta ttcatctaac agcaaataag ttatggcaaa
     1141 aattataggt atagacttag gtactacaaa ttccgcggtc gcggtcatgg aagtcggcga
     1201 gccgaagatc atcgagaacg ccgaaggcgc ccgcaccacg ccgtcgatcg tcgcggaatc
     1261 caagaccggc gagcgcatcg tgggccttct cgcgaagcgc caggcggtta ccaaccccaa
     1321 gaacacaatt ttccagatca agcgcttcat cgggcacacc tttgacgatc cgggcgtgca
     1381 gaaggacatc aagtccgtcc cgttcgaggt gcgcaaaagc gcaaacggcg gcgtagaagt
     1441 gaagatgggc gaacagtggt atcggccgga ggaaatctcg gcgatgatcc tgcagaaact
     1501 caaggcagac gcggaggcgc gcctcggcga gaagatcacc gaagcggtca tcaccgttcc
     1561 ggcatacttc aacgacagcc agcgccaagc caccaaggac gcaggcaaga tcgcgggcct
     1621 cgaggtgaag cgcatcatca atgagccgac tgcggcggcg ctcgcgtacg gcttcaacaa
     1681 gaaaaaagag gagaagatcg cggtcttcga tttcggaggc ggcacgttcg acatttccgt
     1741 ccttgaagtc ggcgacgacg tcatcgaagt gaaatcgacc gacggcgacg cccacctcgg
     1801 aggaaaggat atcgaccaga aaatcatcaa ctggcttgcc gaggaattca aaaaggagaa
     1861 cggtgtggat ctcatgagcg atccgctggc agtccagcgc ctcgacgatg cggccgaaaa
     1921 ggcgaaaatc gaactttcga ccgcggtgca gactgaggtc aatattccgt ttatcagctc
     1981 cggtccgcag ggcccgatgc atctcgtgaa gacgatgact cgcgccaagc tcgaggagct
     2041 cacgggcgaa ttcgtcgaac gcgcaatgtc gatcactaag cgcgccatcg aagcttcgcc
     2101 gtataaggtc ggcgagatca acgaggtgat cctggtcggc ggccaaaccc gcatgccggc
     2161 gatccagcag gccgtaaaag ccttcttcgg caaggaaccg cacatgggcg tcaatccgga
     2221 cgaagttgtc gccttgggcg ccgcaatcca gggcggaatt ctcggcgggg aagtgaaaga
     2281 cgtgcttctc ctggatgtca tccccctgtc cctcggcatt gagacgatgg gcggcgtcgc
     2341 gaccaagctc atcgagcgca acaccaccat ccccagttcg cgctcgcaga tcttctcgac
     2401 ggcggccgac agccagacga gcgttgagat tcatgtggtt cagggcgagc gcgccatggc
     2461 atccgataat aagtcgctcg gcaaattcat gctcgacggc attccgccgg cgccccgcgg
     2521 cgtcccgcag gtcgaagtga ccttcgatgt cgacgcgaac ggaatactta acgtcaaggc
     2581 gaaggagaag acgacaggca aggagcagtc gatccgcatc gaaggaagtt cggggctctc
     2641 gaaggaggac attgagcgca tgcagaaaga cgctgagatg cacggcgacg aagacaaaaa
     2701 gaagaaagag gaagcggaag ccaagaacct cgccgagcag atgatctacg cggcagagaa
     2761 gtcgctcaag gattttggcg acaaggtgag cgaagacatc aaaaagggcg tccaggagaa
     2821 aatcgacgcg ctcaaaaagg aaaaggacgg cggaaatatc gaggctatca agaccgcaac
     2881 cgactcgctc tcggcagaaa tgcagaagat cggccaggcg atgaaccaga atcaaaccaa
     2941 taacccggaa cagaagacgg ctgacaacca aacagggacg caggaaaatg tcagggatgc
     3001 cgacttcgag gaaaagaaag agggcgagaa tcccgaaggc ggccagcaaa aacaatagat
     3061 aagaaaagat cggcaggctc atgggagcct gccgattttg tctatgccgc gagttgtaaa
     3121 aattgcggct tagtgtcggg ttcttccttg ggggaag
//
LOCUS       DASZYS010000035        15118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_679256, whole genome shotgun sequence.
ACCESSION   DASZYS010000035 DASZYS010000000
VERSION     DASZYS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15118
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679256"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VHF05_02080"
     CDS             complement(<1..1044)
                     /locus_tag="VHF05_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVS79743.1"
                     /translation="MKYSLNIQSVTEGKPSFKTALKKFRPIIEGEKKNLIFAGIAILA
                     SSALTLVSPFIVGHVIDTYVVTGRYRGVLLFGGLLLVIYIAAFAASYFQTQLMGAVGQ
                     RTLFNLRNAVFRKLQELPVAFFNQNKSGDLISRINNDTDKLNQFFSQALMQFVSNAFI
                     IVGAGIFLLSINWKLGVAALLPGLFLFIFTRAVGGWIRKKNAVSLQATGGLSAEIQES
                     IENFKVVVAFDRRDYFRQKFGDANRKNYETSVVAGIANNIYIPVYGMVSNAAQLVVLA
                     FGIYLISTGNFTIGLLISYLSYVNRFYDPLRQIASIWATFQVALASFDRISAILSLES
                     DIVVIPAALKPSTE"
     gene            complement(1171..2937)
                     /locus_tag="VHF05_02085"
     CDS             complement(1171..2937)
                     /locus_tag="VHF05_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVS79744.1"
                     /translation="MTSKAANAQANVQAPVKQANLFGVLAPYKALVALLIGLALLSNG
                     LNLILPQLIRQGIDAFGAGAFDMKLIVIEFAAASIAIFVFTYLQSIVQTYASERVARD
                     MRERLAGKISEQTYEFVQAAGAGKLLTNLTSDIDAVKMFVSMGIVSLCSSFIIIVGAS
                     VLLFRIDWKLTLAVLAIVPLIAVTFFIVMSKMRPLFKRTQEVIDWLNKVINESIMGSG
                     LIRVLNSQMPEYDKFVEANTNAKDLGLQILRMFAVLIPVISFVANLAVLVILLLGGRF
                     VIEGAMTLGDFAAFMSYLSLLIFPIIVIGFMSNVISRAQASYQRVAETTEAAAPPRGG
                     AIDRELRGDIAVEGLTVTYGEKSALKNVSLSIHAKTRTAIIGPTAAGKTQLLYALTGL
                     LTPQSGAILFDGIGLDEYDPSSLRRQAVLVFQDSVMFNMTLRDNIAFGGEIKNDDFDR
                     AVKTAELADLVAALPEGFDTLVSERGTSLSGGQKQRIMLARALALNPKILFLDEFTAR
                     VDAATERKILQNIRANYPDLTLVSVTQTIAPVMDYDTIALIMEGEILAQGTHKELMES
                     SPEYVQIFESQKSTSHYETAEQ"
     gene            complement(2998..3156)
                     /locus_tag="VHF05_02090"
     CDS             complement(2998..3156)
                     /locus_tag="VHF05_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79745.1"
                     /translation="MKIRWNKVTTVTKMIAVFLVGLSFVWGLHLGLTYQAAIDGQENL
                     AASAAAAK"
     gene            complement(3240..3824)
                     /locus_tag="VHF05_02095"
     CDS             complement(3240..3824)
                     /locus_tag="VHF05_02095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HVS79746.1"
                     /translation="MRSRSAINSAITEPYEAAVRRSARARRLSVTVNPDGTVVVSAPF
                     RASERAILRFLETSAPWVKKTLARVQRKGSELLPLPEGAGTKKHFATHKERARKILRA
                     RMEYWRVKLDTDFTGPRIGNQSTRWGSCSKTGTISLNYRLVFLPEELMDYVVVHELCH
                     RLHFNHSKSFWCAVGSALPEYGVLRRTLRRYALN"
     gene            complement(3802..4440)
                     /locus_tag="VHF05_02100"
     CDS             complement(3802..4440)
                     /locus_tag="VHF05_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79747.1"
                     /translation="MKKDRTWWWVGGIIVLIIVLVVIGKLTSSSGAKGAASTTIADPS
                     SLPGIQTGTTTPWAVELQNLRARLAADNLPALAAEGTVLHIHQHLDIFIHGQHVTIPA
                     GVGIYEAAQFISPIHVHDLTGIIHVESPTPGTFTLGQFFDIWGVKFTNDCIGGYCNDG
                     TNTLKMYVNGDEYTGDFRTLELKAHDEIAITYGTDAEKPAQIPSSYAFPLGY"
     gene            complement(4459..4863)
                     /locus_tag="VHF05_02105"
     CDS             complement(4459..4863)
                     /locus_tag="VHF05_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HVS79748.1"
                     /translation="MKSFIVRDFLQRLGPSSILVVFGVWEIIDPAYWASYLPGFAANF
                     GHPEAQVVVHGCILAVLGLALLFNFHTRLAAILSTLVLLSIVFSLLLEGGWNEIVMRD
                     ISILISTSALMFPSIAGNGRAREAHEAAHDNL"
     gene            complement(4938..5843)
                     /locus_tag="VHF05_02110"
     CDS             complement(4938..5843)
                     /locus_tag="VHF05_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79749.1"
                     /translation="MNSKFLPYVMLAAAVFVFIVSAFVVRNMYLPPAAAKAATSKIPA
                     AAAAGSVSASGSGSVPAGVADTKASPAASSAPLPSDGPIVSINFDDGFESAYQLGLPI
                     LDKAGLKATWYIITGTSTALDTKGYMTTEQLLALQADGQEIGAHTRHHLSLGLITDPN
                     ELRYEILGSKEDLLAMGIKDVSTFAYPEGSFSPLAEEVVKESGFTGARITRPLLNNKT
                     VDPYLLKYQRVQYDTTWADIQSAIDQAIAKKEWVILVFHRLDETGNEQSAPSALLQQT
                     VDYIKQKNVPVVTNEDGLKILGNIK"
     gene            6032..6226
                     /locus_tag="VHF05_02115"
     CDS             6032..6226
                     /locus_tag="VHF05_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HVS79750.1"
                     /translation="MWQQWVNFILGLWIIVSAYAGMTGAALTTNLLVVGVLVAILALW
                     GALQAGSHERHLHEGHYKST"
     gene            complement(6279..7457)
                     /locus_tag="VHF05_02120"
     CDS             complement(6279..7457)
                     /locus_tag="VHF05_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HVS79751.1"
                     /translation="MKSKAFKGFLAISILLSAAWMVPHFAAAQESGTVVPDTVEVVKA
                     RVIDIQKEEEQVIPGTDTPGLFQTIEAEILEGSQKGKIVTFTDDYLHLQKGDIFYLNH
                     TVQTMDGIETYAEMDAYRMPTIYLFGGMFVLLVLLIGGMQGLRGLASLTGSLLLIFFV
                     LIPGIIGGLSPVLVSVVVSAVIIIVGSYITHGFNKTTSSAVVGMIITVLFTGALAYWA
                     VHMGRLTGYGTDESVYVTLNSRGKIDILGVLLGGMMIGFLGILYDVAIGQAISVEELH
                     HIAPHVEKKRIYSRALRIGREHIGALVNTLAIAYVGASLPLLIVYAQSSTPISQIANR
                     EVFATEIIRTFVGSIGLVLAVPITTFLAVMILVKRRGAADKEIIHEEREALKHYEHHH
                     "
     gene            complement(7486..7989)
                     /locus_tag="VHF05_02125"
     CDS             complement(7486..7989)
                     /locus_tag="VHF05_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79752.1"
                     /translation="MTLTTHALVGAAAASLFPNHPVAAFVAGFASHLAIDSIPHWDYT
                     ILSMEQKRDAPLQNDMNFGKLFAIDLLRIGFDCLLGLVFSVVIFSFFLGLVSPEVALI
                     GAVAGILPDPLQFAYMKTRSRILLPLQRFHLWLQKGKQLSISPLKGISFQLAIVVAAS
                     LIVILIR"
     gene            complement(8000..8572)
                     /locus_tag="VHF05_02130"
     CDS             complement(8000..8572)
                     /locus_tag="VHF05_02130"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVS79753.1"
                     /translation="MEIFIYLAAACWATFLIVWAVTSFGIKKSVGGWGRWLAVRAILL
                     AVIFAVLGNKIFTGDSVHMSRASGPANIPLGVIGVLLCMAGIGFAIWARKHLGKNWGM
                     PMSIKEKPDLVTTGPYTYVRHPIYTGMLLALAGSAIVGGQWEWLVPAALFAAYFVYSA
                     KVEEKNMSKIFPDQYPEYKKRSKMLIPFVF"
     gene            8678..9016
                     /locus_tag="VHF05_02135"
     CDS             8678..9016
                     /locus_tag="VHF05_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVS79754.1"
                     /translation="MREESIEQKEAQMRKGLLEFCILLAVSKDRVYSSDILKKLKDAD
                     LLVVEGTLYPLLSRLKQQGLVDYDWEESPSGPPRKYYTLTKRGKDMLILLEKTWTALR
                     SSVMSLTRSS"
     gene            9037..10770
                     /locus_tag="VHF05_02140"
     CDS             9037..10770
                     /locus_tag="VHF05_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HVS79755.1"
                     /translation="MEKTTHITIASTEFRLTESAYDKLEKYLSALSEHFKGEEGGAEI
                     IRDIESRIAEKLLETKHGLITLANVEAVIAEIGNPAELDVPIEEALQEGVRKYRKLYR
                     NMDYAVLAGVASGIAAYFGIDPLVIRLIFVISIFFGGIGIVIYVLLAIFVPAAHNASQ
                     KLEMRGDAVTLDGISRVVKDRSSEEKRESGFRRVIYFPFEVIAAIGRFIANRLSPVAL
                     RIAGLLLALVTFLAALALTVAVGVAWANWNAPWNDFPLKPVMSGGLMIFTSLMSYLAV
                     IIPLFLIFALGFRLFRRRNFLPSAVGIGLVGIWSLALIAAGITWTKVGSDYHEYEQTS
                     PEYQIVERTLDVTGFTKIEAHDVRVAFSSEGTSTVSIEGREADLGSVTATTTPDGTLV
                     LTGNDQLEASCFFMCFHSRPTVIISTSSVSAIDIDDSSLTFDDFEGGAISLKAKDSNV
                     RGTLHANSLSLELEDSGAYATVDLASLQADLRDSHLEFDGTANTATINAVSSSVDSSS
                     LFTNAMTLSTQGSSANVSAENFTLVKSTQSTVYNNDKLVGGDDDEKSAGGRGEESSRA
                     PTAPLAPEVQE"
     gene            complement(10805..11326)
                     /locus_tag="VHF05_02145"
     CDS             complement(10805..11326)
                     /locus_tag="VHF05_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79756.1"
                     /translation="MRTSTIVWIIVIIVVILGGTYWWAASNSANTPLVTDTSQAPMIP
                     AGSSTEPTGTGNAYAPGNLLLGTDATSTLGTYLVASNGMTLYTYANDTSGVSNCSGQC
                     AANWPPYTVASADALANVQSGITGQVGTITRDDGKLQVTYNGMPLYFWANDKAPGDTN
                     GQGVGNVWFVVQP"
     gene            11441..12034
                     /locus_tag="VHF05_02150"
     CDS             11441..12034
                     /locus_tag="VHF05_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79757.1"
                     /translation="MGSIHAMKEGRKFYIIPGLGESTRAQNYREIIKGIRASGFDIVP
                     INLEWDLDVPFSEFVKKADKQIPNNIAEDYILGFSFGAYIAALLAPKKKACGYFFCSI
                     SPYFKDDLQYIPQETKDYFGTTFMSSFKKYSFPENVSGNAWFFIGDKDWEIAQERARD
                     SYNKWRGGKEMFIVKGAGHELKDPNYTKEVLNVIKKL"
     gene            complement(12810..13121)
                     /locus_tag="VHF05_02155"
     CDS             complement(12810..13121)
                     /locus_tag="VHF05_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79758.1"
                     /translation="MSNFFKKYLSYLKNNPGHYWFKDKLYGWGWVPATWQGWLVIALY
                     VVILVLLALTIDSNSSRREVAFMFILPVVILTTLLLVIIYKKGERPRWQWGNPPDREE
                     K"
     gene            complement(13157..14275)
                     /locus_tag="VHF05_02160"
     CDS             complement(13157..14275)
                     /locus_tag="VHF05_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HVS79759.1"
                     /translation="MKLSSLNKKFISYCVKKRLGRNNFTIKVIYSITGLLGRFEVYKN
                     SHEFQKRGHYIPQFLLRRFRLSELRHDPNRKKIYQYNFLFNTLTEEKIEVVAQIEDFY
                     IFKQNGGGSSDYVEKVILADWIENFGSQILKTINTTEDDPNLTLLEKNILATFISHQI
                     TRTPAFYFQLRKYILFLYEKKLLKIDNLSDHNFLKEIIVKNKYNFTYDDLVNFVPEYT
                     MTGDLNHLGFIARLIAGEITEGIFRHNFYFLNIPDTSDDEFVISDNPVIFVDFQKFEI
                     KRYFDWWSKSSGKTWIFMPISPKKCLFLTTKQKIDGRIENDQADMVALVNFGQYLNAL
                     NYVYGRNKNLMLQNLRKYLPELLRYKTIIADPIEILNK"
     gene            complement(14358..14729)
                     /locus_tag="VHF05_02165"
     CDS             complement(14358..14729)
                     /locus_tag="VHF05_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79760.1"
                     /translation="MLQRLSNPKNPFGFSGVFSISGIALTFRRDAYDALKQSEHHLNF
                     PLEILLTISKTYSRLIDINLFVTTMVKTWEASVEEAKRENIDSQVVSDQRKKHTRTLF
                     QSVRAIALEAQTDLCDLLAKL"
     gene            complement(15050..15118)
                     /locus_tag="VHF05_02170"
     tRNA            complement(15050..15118)
                     /locus_tag="VHF05_02170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15085..15087),aa:Gln,seq:ttg)
BASE COUNT         3700 a         3871 c         3949 g         3598 t
ORIGIN      
        1 ttccgtgctc ggcttcagag ccgcgggaat gacgacgatg tccgactcaa gcgaaaggat
       61 cgccgagata cggtcgaagc tcgcgagcgc cacctggaag gtagcccaaa tcgacgcgat
      121 ctgacgaaga ggatcataga acctgttgac gtaggaaaga tagctgataa ggaggccgat
      181 ggtaaagttg ccggtcgaga tgaggtagat accgaacgcg agcaccacga gctgggcggc
      241 gttcgaaacc atgccgtaga ccggaatgta gatattgttc gcgatgccgg ccaccacgga
      301 ggtctcgtag ttcttgcgat tggcatcgcc gaatttctgc cggaaataat cccgcctgtc
      361 gaacgccacc acgaccttga agttctcgat cgattcctgg atctcagccg agaggccgcc
      421 ggtcgcctga aggctcaccg cgttcttctt tctgatccag ccgccaacgg cacgagtgaa
      481 tatgaaaagg aaaagccccg gaaggagcgc cgcaacgccg agcttccagt ttatcgaaag
      541 caggaagatt cccgcaccga caatgatgaa cgcgttcgag acgaactgca tcagggcctg
      601 cgagaaaaat tggttcagct tgtcggtgtc gttgttgatg cgcgaaatga gatcgcccga
      661 cttgttctgg ttgaagaacg ccacgggaag ctcctggagc ttccggaaaa ccgcgtttcg
      721 gagattgaag agcgtcctct gacccaccgc gcccataagc tgggtctgga agtagctcgc
      781 cgcgaacgcg gcgatataaa tgacgagaag aaggccgccg aagaggagca cgccgcgata
      841 gcgccccgtc acgacgtagg tatcgattac atggccgacg ataaacggcg atacgagcgt
      901 aagcgccgag ctcgcaagga tggcgatgcc cgcgaagatg agattcttct tctcgccctc
      961 gatgattggc cggaatttct taagggccgt cttgaacgac ggcttccctt ccgtgacgct
     1021 ttgtatgttg agtgaatatt tcatcccgtt agagatagga aacggccgcc gtttccgttc
     1081 ttgaaaattg ctttgtaaaa atatcgacca tgcttcttaa attatttccg aaaaattcat
     1141 ataactgaaa tatctctaac gggattcatt ttattgctcc gcggtttcgt agtggctcgt
     1201 gctcttctgc gactcgaata tttgcacgta ttcgggcgac gactccatga gctccttgtg
     1261 agttccctgc gccaatatct cgccttccat aatgagggcg atcgtatcgt aatccatgac
     1321 cggcgcgatg gtctgcgtca cggatacgag ggtcagatcg ggataattgg cgcggatatt
     1381 ctggagaatc ttgcgctcgg tcgccgcatc gacgcgggcg gtgaactcgt cgagaaagag
     1441 aattttcgga ttgagtgcca gcgcgcgagc gagcataatg cgctgtttct gtccgccgga
     1501 aaggctcgtg ccgcgctcgg aaacgagcgt atcgaagcct tcggggagcg cggccaccag
     1561 gtcggcaagt tcggcggtct tcacggcgcg gtcgaaatca tcattcttga tctcgccgcc
     1621 gaaggcgata ttgtcgcgga gcgtcatgtt gaacattacg ctgtcctgaa agacgagcac
     1681 agcctgcctg cggagcgagg acggatcgta ttcgtcgagc ccgattccgt cgaaaaggat
     1741 agcacccgac tggggcgtaa gaaggccggt cagcgcatag aggagctggg tctttcctgc
     1801 cgctgtcggc ccgatgatcg cggtgcgggt cttcgcgtgg atcgagagcg acacgttctt
     1861 gagcgcggac ttctccccgt aggtgacggt aagcccttcg accgcgatgt cgccgcgaag
     1921 ctcgcggtcg atcgcgccgc cccgaggcgg cgccgcggct tcggtcgttt cggcgacgcg
     1981 ctggtacgac gcctgcgcgc gggagatgac gttcgacatg aatccgatga cgatgatcgg
     2041 gaaaatgaga agcgagagat agctcatgaa cgcggcgaaa tctccgagcg tcatcgcgcc
     2101 ctcgatgacg aacctgcctc cgaggaggag gatcaccagc accgcgagat tcgcgacgaa
     2161 cgagatcacc ggaatgagca ccgcgaacat cctgagaatc tggaggccga ggtccttcgc
     2221 attcgtattc gcctcgacga acttatcgta ctcgggcatc tgcgaattga gcacccgaat
     2281 gaggcccgaa cccatgatgc tctcgttgat gactttgttc agccagtcga tgacttcctg
     2341 ggtgcgcttg aagagcggcc gcattttgga catcacgatg aagaaggtga cggcgatgag
     2401 cggcacgatg gcgagcacgg caagcgtaag cttccagtcg atcctgaaaa ggaggacgct
     2461 cgcgccgacg atgatgatga aggacgagca gagcgagacg attcccatcg atacgaacat
     2521 cttcaccgcg tcgatgtcgg acgtgagatt ggtcaggagc ttgcccgctc ccgccgcctg
     2581 gacgaattca tacgtctgct ccgatatctt tccggccaat ctttcgcgca tgtcgcgggc
     2641 cacgcgctcc gacgcgtagg tctggacgat gctctgaagg taggtaaaga cgaatatggc
     2701 gatcgaggcg gcggcgaatt cgatgacgat aagcttcatg tcgaaggcgc cggcgccgaa
     2761 cgcgtcgatt ccctgcctga tgagctgggg aaggatgaga ttcagcccgt tgctcaaaag
     2821 ggcgaggccg atgaggagcg cgacgagagc cttgtaaggc gcaagcacgc caaaaagatt
     2881 cgcctgcttg acaggcgctt ggacgttcgc ttgcgcattc gcggcttttg aagtcattat
     2941 ttagacaccc taacattttt cgcggccaca gtcaaagacg cactgcggcc cctcgagcta
     3001 cttcgccgca gcggcgctcg cggcaagatt ctcctgaccg tcgatagcgg cctggtaggt
     3061 caggccgaga tggaggcccc acacgaacga gagtccgaca aggaacacgg ctatcatttt
     3121 cgtgacagtc gtcactttat tccatcgtat tttcatatta tttcctaaat aaaaactgca
     3181 ctgatgagtt aattatatat gaaacggctc ttgatcgaag ctcggcctgt gaattgcatt
     3241 caattgaggg cgtaacgcct gagcgtgcgc cggagaaccc catactcggg cagcgcgctc
     3301 cccacggcgc accaaaaact cttcgagtgg ttgaaatgga gcctgtggca gagttcgtgg
     3361 acaacgacat aatccataag ctcttcggga aggaagacca gccggtaatt gagggaaatc
     3421 gttccggttt tcgaacaact tccccatctc gtcgactggt ttccgattct tggtccggta
     3481 aaatcggtgt cgagctttac cctccagtat tccattcgcg cccgcaaaat cttccgcgcc
     3541 cgctccttgt gcgttgcgaa atgctttttc gttcccgccc cttcgggaag cggcaggagc
     3601 tcactcccct tcctttgcac ccgcgcaagc gtctttttca cccatggggc ggaagtttcc
     3661 aagaaccgca aaatcgcgcg ttcggatgcg cgaaaggggg cggagacgac cacggtgccg
     3721 tccggattca cggtgacgga gagcctccgc gcacgggcgc tgcgcctgac agccgcctcg
     3781 tagggttcag tgatcgcgga attaatagcc gagcgggaac gcatagctcg acgggatttg
     3841 cgcgggcttt tcggcatcgg tgccgtaggt gatcgcgatc tcatcgtgcg ccttgagctc
     3901 gagagtgcgg aaatcgcccg tgtactcgtc tccgttcaca tacatcttca atgtattcgt
     3961 gccgtcgttg caatagcccc cgatgcagtc attggtgaat ttcacgcccc aaatatcgaa
     4021 gaactggccg agcgtgaacg tgccgggagt cggcgattcg acgtggatga tgccggtcag
     4081 atcgtgcacg tggatcggcg aaatgaactg cgccgcctcg tagatgccga cgcccgcggg
     4141 aatcgtaaca tgctggccgt gaatgaagat gtcgagatgc tggtggatat ggagcaccgt
     4201 gccttcggcg gcgagggcag gaagattgtc ggcggcgagg cgagcgcgga gattttgaag
     4261 ctcgaccgcc catggcgtcg tggtgcccgt ctggatgccc ggaagcgacg aaggatccgc
     4321 gattgtggtc gaagcggccc ccttcgctcc cgaagaggac gtgagtttgc cgatgacaac
     4381 aagaacgatt atgagaacga taatccctcc aacccaccac cacgtacggt cttttttcat
     4441 gtgcgattga aaaataaatt acaaattatc atgtgccgct tcatgcgcct cgcgcgcacg
     4501 gccgtttccc gcaatcgaag gaaacatgag cgccgaggtc gagatgagga tcgaaatgtc
     4561 ccgcatcacg atctcgttcc agcctccttc gagaaggagc gagaagacga tcgagagaag
     4621 caccagggtc gaaaggatcg ccgcaagcct cgtgtggaaa ttgaaaagaa gcgcgagccc
     4681 caacaccgcc agaatgcagc cgtgaacgac cacttgggcc tccggatggc cgaaattcgc
     4741 ggcaaagccg ggaagatagc tcgcccagta cgccgggtcg atgatctccc acacgccgaa
     4801 gacgacgaga atcgatgacg gtccgagtct ttgcaggaag tcgcgtacaa tgaagctctt
     4861 cattctcttg attgcgggtt cttaaaacct caatgccccc attttaccac accgtcgcgc
     4921 tatgcgcctg tgaatggcta tttgatattg ccgagtatct taaggccgtc ctcgttcgtt
     4981 acgacgggaa catttttctg cttaatatag tcgaccgtct gctgaaggag ggcgctcggg
     5041 gcgctctgct cattgcccgt ttcgtcgagc ctgtgaaaga cgagaatgac ccattccttt
     5101 ttggcaatcg cctggtcgat cgcgctctgt atgtcggccc aggtcgtgtc atactgaacc
     5161 cgctggtatt tgagaagata ggggtcgaca gttttgttat tgagaagcgg gcgcgtgatg
     5221 cgtgcgccgg taaagccgct ttctttcact acctcttcgg caagcgggct gaagctccct
     5281 tcgggatacg cgaaggtcga aacatcctta atgcccattg cgaggagatc ctccttggag
     5341 ccgagaattt cgtatcgaag ctcgttcgga tcggtaatca agccaagcga aagatgatgg
     5401 cgagtatgcg cgccgatctc ctgtccgtcc gcttggaggg caagaagctg ctccgtcgtc
     5461 atgtagcctt tcgtatcgag ggcagtcgaa gtgcccgtta tgatatacca cgtcgcctta
     5521 aggccggcct tgtcgaggat cggcaggccg agctggtatg ccgactcgaa gccgtcgtcg
     5581 aaatttatgg agacgatcgg gccgtcggaa ggaagcgggg cggacgacgc ggcgggagac
     5641 gctttcgtat cagcgacacc tgccggaacc gagccgctgc cggaggcgga aacgcttccc
     5701 gccgcggccg ctgcgggtat ttttgatgtc gcggccttgg ccgcggccgg cgggagatac
     5761 atatttctca cgacgaacgc ggaaacgata aagacgaaga ccgccgccgc aagcatgacg
     5821 tatggaagga atttcgaatt catattttat aacggcaaac ggggttggtt ttcttgcgct
     5881 caattcttgc gaatgcctat gaagatttcg tgtgattttt tgcgcgtttt gaagctgttt
     5941 cgctcccccg ctttctttat catttcttta gattgatgaa ataacatgtg gatagggtcg
     6001 attttttaaa attttaagaa atcaaaatcg tatgtggcaa cagtgggtta atttcatcct
     6061 aggtctctgg attatcgttt cggcatatgc cggaatgaca ggcgccgcgc ttaccaccaa
     6121 ccttctggtg gtcggggtat tggtcgccat tcttgctttg tggggcgctc ttcaagccgg
     6181 ttcgcacgag cgtcacttgc acgaaggcca ctacaaatcg acttaggagt tctcgaagaa
     6241 gcggcgggcc agaggcccgc tgttttttca tatccggctt aatgatgatg ctcgtaatgc
     6301 ttgagcgctt cccgctcctc gtgaatgatt tccttgtcag ccgcgccgcg cctcttcacg
     6361 aggatcatga cggcgaggaa ggtcgtgatc ggaacggcaa gcacgagacc gatcgaaccg
     6421 acgaacgtcc gtatgatctc ggtcgcgaaa acttccctat tggcgatctg cgatatgggc
     6481 gtcgacgact gcgcgtagac gatgaggagc gggagcgaag ctccgacata ggcgatggcg
     6541 agcgtgttca cgagcgcgcc gatatgctcg cgcccgatgc ggagcgcccg ggaataaatc
     6601 cgcttcttct cgacgtgcgg cgcgatatgg tgcaattcct cgactgatat ggcctggcct
     6661 atggcgacgt cgtacaggat tcccaggaag ccgatcatca ttcccccgag aagcacgccc
     6721 agaatgtcga tcttgccgcg gctgttcaag gtcacataaa cggattcgtc cgtgccatag
     6781 ccggtcaggc ggcccatgtg caccgcccaa tacgcgagag ctccggtaaa gaggacggta
     6841 atgatcatgc cgaccaccgc cgaggaagtg gttttattga agccgtgcgt tatatacgaa
     6901 cccacgatga tgatgaccgc ggaaacgact acgctcacga gcaccggaga gaggccgccg
     6961 attatgccgg ggatcagcac gaagaaaatg agaagcaggc tgcccgtcag acttgccaag
     7021 ccgcgaagac cttgcatgcc gccgataaga agcacgagca ggacgaacat tccgccgaaa
     7081 agatagatgg tcggcatgcg atacgcatcc atttctgcgt atgtctcgat gccgtccata
     7141 gtttgaaccg tgtggttgag gtagaagata tcgccctttt gaagatggag gtaatcatcg
     7201 gtgaacgtga caatcttccc cttttggctg ccctcgagta tttccgcctc gatagtctga
     7261 aacaggccgg gagtgtccgt tccgggaata acctgctcct cctctttctg aatatcaatg
     7321 acgcgcgcct tgacaacctc gacggtatcc ggaactacgg tcccgctctc ctgcgcagcg
     7381 gcgaaatggg ggaccatcca tgccgctgac aaaaggatcg aaatggcgag aaaaccttta
     7441 aaagctttcg atttcatgga agaagtgatg ccgcgcgaac gtggtttatc ggataaggat
     7501 tacgataagg ctcgcggcga cgacgatcgc aagctggaag gaaattccct tgagaggcga
     7561 gatgctgagc tgcttccctt tctgaagcca gaggtggaag cgctggagcg gcaggaggat
     7621 tctgctccgc gttttcatgt atgcgaactg gagaggatcg ggcaagatgc cggcgaccgc
     7681 gccgatgagc gcgacctccg gcgagacgag tccgagaaag aacgagaaga tgacgacgga
     7741 gaaaacgagc cctagcaggc agtcgaagcc gatgcgaaga aggtcgatcg cgaagagctt
     7801 gccgaagttc atgtcgttct ggagcggcgc gtcgcgtttt tgctccatcg agaggatcgt
     7861 ataatcccag tgcggtatcg aatcgatcgc caggtggctc gcgaatcccg cgacaaacgc
     7921 ggcaacggga tgattcggaa agaggctcgc ggctgccgca ccgacaaggg cgtgagtggt
     7981 aagcgtcatg attgaaaaat taaaagacga acggaatgag catcttgctc cttttcttgt
     8041 attcgggata ctggtcggga aatattttgc tcatattttt ctcctcgacc ttcgcgctgt
     8101 atacgaaata ggccgcgaag agcgcggcgg gcactaacca ttcccattgc ccgccgacga
     8161 tcgcggaacc cgccagcgcc aggagcattc ccgtgtagat cggatgccgg acgtatgtat
     8221 acggcccggt ggtaacgaga tcgggctttt ccttgatgct catgggcatg ccccagttct
     8281 tgccgagatg cttgcgtgcc cagatcgcga atccgattcc ggccatgcaa agcaggactc
     8341 cgataactcc gagaggaatg tttgccggac cgctcgcccg gctcatgtgg acgctgtctc
     8401 cggtaaagat cttatttccg agaaccgcga agataacggc gagaaggatc gcgcgcacgg
     8461 caagccagcg tccccagccg cccactgact tcttgatgcc gaacgacgtg actgcccaca
     8521 cgatgagaaa agtcgcccag catgcggcgg cgagatatat gaagatttcc atacgaaaag
     8581 tatagcagat ttcaagctat ggaagcgccc ttcggccgcc gataacgcta tacacacagt
     8641 accttgcact acaagctact gtgtgtataa tataactatg cgagaagaat ccatcgaaca
     8701 aaaagaggcg cagatgcgca aagggctttt ggagttttgc atcttgctcg cggtctccaa
     8761 ggatcgcgtg tactcgtcgg atattttgaa gaagctcaag gatgccgacc tcctggtggt
     8821 ggaaggcacg ctctatcccc tcctctcccg attgaagcag cagggcctcg tcgattacga
     8881 ttgggaggaa tcgccgtccg ggccgccgcg caaatattac acgcttacca agcggggcaa
     8941 agacatgctt atacttctcg agaaaacgtg gactgcactc cggtcgtcag tcatgtcgct
     9001 tacccgttcc tcataactta tcaatcaatt atcaatatgg aaaagacaac ccacataacg
     9061 atcgcctcga ccgagttccg gctcacggag tccgcgtacg ataagctcga gaagtatctt
     9121 tccgcgcttt ccgaacattt caaaggcgag gaaggcggcg ctgaaatcat ccgcgacatc
     9181 gaatcgcgca tcgccgaaaa gcttctcgaa acgaagcacg gcctcataac gctcgccaac
     9241 gtcgaagcgg tcatcgccga gatcggcaat cccgccgagc ttgatgtccc catcgaggaa
     9301 gcgctccagg aaggcgtacg caaatatcgc aagctctacc gcaatatgga ttatgcggtc
     9361 cttgcaggcg tggcgtccgg catcgccgca tatttcggta tcgatcctct cgtcatcaga
     9421 ctcattttcg tcatttcgat cttcttcggc ggcataggca tcgtgatcta tgtccttctc
     9481 gccatcttcg ttcccgcggc gcacaatgcg agccaaaagc tcgagatgcg cggcgacgcg
     9541 gtaacgctcg acggcatcag ccgcgtggtg aaggaccgct cgtcggagga aaagcgcgag
     9601 agcggattcc gccgcgtcat ctacttccca ttcgaagtca tcgccgcgat tggccgcttc
     9661 atcgcgaacc gcctttcccc tgtggcgctc cgaatcgcgg ggctccttct cgcgctcgta
     9721 acgttccttg cggctctagc cctcactgtc gctgtgggtg ttgcgtgggc gaactggaac
     9781 gccccctgga acgattttcc cctgaagccg gtgatgtcgg gcggtttgat gatcttcact
     9841 tcgctcatgt cgtatcttgc ggttatcatt ccccttttcc tcatcttcgc attgggcttc
     9901 cgccttttcc gccgcaggaa tttcctgcca tccgccgtcg gtatcggcct cgtaggaatc
     9961 tggtcgctcg cgctcatcgc ggctggcatc acatggacga aggtcggctc cgattatcat
    10021 gaatacgagc agactagccc cgaataccag atcgtcgagc gcacgctcga cgtgacggga
    10081 ttcaccaaaa tcgaagcgca tgatgtccgc gtagcgttca gctctgaggg cacgtcgacc
    10141 gtttcgatcg aaggtcgcga ggcggatctg ggaagcgtta ccgccaccac cacgccggat
    10201 gggaccctcg tccttacggg caatgaccag ctggaagcat catgcttctt tatgtgcttc
    10261 cattcgaggc cgacagtcat catctccacg tcttcggttt cggctatcga cattgatgac
    10321 agctcgctca ccttcgacga tttcgaaggc ggcgccatct ccctaaaagc caaggactcg
    10381 aacgtacgcg gcaccctcca tgcaaactcg ctatcgctcg agctcgaaga cagcggcgcg
    10441 tacgcgaccg tcgatctggc ctcgcttcag gcggatctgc gcgattccca tcttgaattc
    10501 gacggcacag ccaataccgc gacgataaac gccgtatcct catcggttga cagcagttcg
    10561 ctcttcacaa acgcgatgac gctctcgacc caaggttcgt cggccaacgt gagcgccgaa
    10621 aatttcacgc tcgtgaagag cacgcaaagc accgtttaca acaacgacaa gctcgtaggc
    10681 ggcgacgatg acgaaaaaag cgccgggggc agaggcgaag aatcgtctcg cgcgcctacg
    10741 gcgcctctcg cacccgaggt gcaggagtaa tatttctccc ttttttctgc ctcggatcta
    10801 aaccctacgg ctgtacgacg aaccatacgt taccgactcc ctgcccgttc gtgtcgcccg
    10861 gagccttatc gttcgcccag aagtagagcg gcatgccgtt ataggttacc tggagtttcc
    10921 catcgtcgcg cgtaatcgtg cccacctggc cggtgattcc cgactgcacg ttcgcgagcg
    10981 catcggccga agcgacagtg tacggaggcc agttggccgc gcactgcccg ctgcaattcg
    11041 aaactcccga cgtgtcgttc gcatacgtat aaagcgtcat gccgttcgac gcgacgagat
    11101 acgtgccgag agtcgacgtt gcgtcggtgc cgaggagaag attgccgggc gcgtaggcat
    11161 tgccggtccc cgtgggctcg gtcgatgagc ccgcgggaat catcggagcc tgcgaagtat
    11221 cggtaaccag cggcgtgttt gccgaattcg aagccgccca ccaatacgtc cctccgagga
    11281 tcacgacaat aatgacgatg atccaaacaa tcgtggatgt tctcataaat tagaataatg
    11341 tgataaatac cgtcggatta agtatacctc cgcctttggc aagcttccat gaacgcaata
    11401 tttaatttta cgagaggtaa tattgcgtat aaatcatagt atgggtagta ttcatgctat
    11461 gaaagagggg cgaaaatttt acataattcc cggattaggc gaatcgacac gggcacagaa
    11521 ctatcgggaa ataataaaag gcattcgtgc ctctggtttt gatatcgtgc cgatcaatct
    11581 cgaatgggat ctggatgtcc ccttctctga atttgtgaaa aaggcggaca agcaaattcc
    11641 gaataatata gctgaagatt atatccttgg attttccttc ggtgcatata tagcagccct
    11701 gcttgccccc aagaagaaag cctgcggata tttcttttgc tcgatatcgc cctacttcaa
    11761 agatgacttg caatacattc ctcaggaaac aaaggattat ttcggcacga cttttatgag
    11821 cagtttcaaa aaatactcat tccccgaaaa tgtgtcaggc aatgcatggt ttttcattgg
    11881 agataaggac tgggaaatag ctcaagaaag ggcaagagat tcctataata aatggagggg
    11941 cggcaaagag atgtttatcg tgaaaggtgc cggacatgaa cttaaggatc caaactacac
    12001 gaaagaagtt ttgaatgtaa taaaaaagct ttgaggtaaa cctcaaagct ttttgacgat
    12061 ctctcgtgct aatagtttaa gcatgcatct cagtgtcttg gccgtccgcg cggcatccac
    12121 cagtcgatga tgaattttcg tttgatctcc ggccatgaag tgatcctacc gaaaagaact
    12181 ctgaatgccc gttctttctc gtcctcaagc gtgcccgcgc aacgccaaga atagatgcgt
    12241 tcaagaattt gcacagccgc ttcgcatgat ggaggattcg gatgccccat gagcgtgttg
    12301 taagcctcgt gatggcccca gattactcta atgataaaag ttccgggttg ccgatacgtt
    12361 cgaggcggaa cgtggtggtg ggtaatcttc gaataattca aacgatactc tcctctcctg
    12421 ccgtaatctt aagcgctctg agaaatttct ggtcctgatc attcaatacc agctttgaaa
    12481 catgttccag tgcgggcagg catttattca gattgaccag ggcaggcaac gaatattcga
    12541 tggtgatgca cgccttgcat cttggacaat cggcgtagat tttccagccc gtatcggtcg
    12601 cattgcacga cttcaggaac aatcgattgc cgcaatgcgg acaaattatc ctataagcgc
    12661 catccatgcg ctctcctttc gtttgcaaag tacgcagata tgaaaaagat cagaactgga
    12721 aatcaatcct gatcatgatc ttatcaagct ctactgtatt cagtttatca tctggcaatg
    12781 acggcgcaaa tcaccttaat cacacattat tatttctctt ccctgtctgg cggatttccc
    12841 cattgccagc gcggccgttc ccctttcttg tagataatga caagaaggag cgtagtaagg
    12901 ataaccacgg gcaagatgaa catgaacgcg acttcgcgcc gtgaagaatt gctatcgatc
    12961 gtaagcgcga gaagcacgag tatgacgacg tagagagcga tcacgagcca cccctgccag
    13021 gtcgccggaa cccagcccca gccatagagc ttatccttga accaatagtg cccaggattg
    13081 ttcttgaggt atgagaggta ttttttgaag aaattggaca taggctaagt ataccgcgag
    13141 tctttcataa tataaattac ttgttcaata tttctattgg atcagcaata atagttttat
    13201 atcgtaataa ttcgggtaaa tattttctta gattctggag cataagattc ttattcctac
    13261 cataaacata attcaatgca tttaaatact gcccaaaatt tactagggca accatatcgg
    13321 cttgatcatt ttcaattcga ccatcaattt tctgtttagt tgttaaaaat aaacattttt
    13381 tcggtgatat aggcataaag atccatgtct tgccacttga cttactccac caatcaaaat
    13441 accgttttat ttcaaatttt tgaaaatcaa caaaaattac tggattatcc gaaattacga
    13501 attcatcatc agatgtgtct ggaatattaa gaaaatagaa attgtgtcta aaaataccct
    13561 ccgtaatttc tccagcgatt aatcttgcga taaagcctaa gtgatttaaa tcacccgtca
    13621 tagtgtattc aggaacaaaa tttaccaaat catcatatgt aaaattatac ttattcttta
    13681 cgatgatttc ttttaaaaaa ttatgatcgc ttaaattatc aatttttagt aacttttttt
    13741 catataaaaa caaaatatac tttcttagtt gaaagtagaa agcaggagta cgagttatct
    13801 gatgtgatat gaaagttgcg agaatgttct tttcaagaag agtaagattt ggatcatctt
    13861 cagttgtgtt tatagttttg agtatttgac taccaaaatt ctcaatccaa tcagccaaaa
    13921 ttactttctc aacataatca gaagaacctc ctccattttg cttaaaaatg taaaaatctt
    13981 ctatttgtgc tactacttcg attttttctt ctgttagcgt attaaacaga aaattatact
    14041 gataaatttt ttttctgttt ggatcgtgcc ttaactctga taatctgaat cttcttaaaa
    14101 gaaattgagg gatataatga cctctttttt gaaattcgtg agaattttta tagacctcaa
    14161 atcgtccaag caagccagta atagagtaaa taacttttat cgtaaagtta ttccttccca
    14221 atcttttttt tacacaatat gagataaatt ttttatttaa agatgatagt ttcataagat
    14281 atggaaatga aattaccaac taatactagt tatatatata aaattaatcc acccgaatac
    14341 aattaaatat atcgagatta cagtttagct aaaagatcac acaaatccgt ttgagcttcc
    14401 aatgcaatag ctcgaacact ttgaaaaagt gttcgagtat gtttttttct ttgatcacta
    14461 acgacttgtg aatctatatt ttctcttttt gcttcttcaa ctgaagcttc ccatgttttg
    14521 accatagttg tcacaaaaag atttatgtct attaatcgtg aataagtttt agatatcgtc
    14581 aataaaattt caagtggaaa attaagatga tgttcacttt gtttgagcgc atcataagca
    14641 tcccttctga aagttagagc tattccagaa attgaaaata ctcctgagaa gccaaatgga
    14701 ttttttggat tactcaatcg ctgcaacata tcatcacagt tttcaattat tgattgaact
    14761 atactgagat tatagtttat ttcatcaacg aaaaacctgg agaattttct tgtgttccgc
    14821 tttcggtgtt gcgagcttac taattcctgg agtaataagg caaggaaaac cccagcaaag
    14881 gttattatta gatttacaaa ccagctgtct gaattaataa tcaagtgatt tatattaaaa
    14941 agtacctata catatattag taagtcagat ctaaattctt ttcacttaaa tatttttgat
    15001 ggaagacata ttgatcattg atacttaaaa agatccctgc gctcctagct gccggacttg
    15061 gattcgaacc aagatactcg cctccaaagg gcgatgtcct accattagac gatccggc
//
LOCUS       DASZYS010000036        14845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_170569, whole genome shotgun sequence.
ACCESSION   DASZYS010000036 DASZYS010000000
VERSION     DASZYS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14845
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170569"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VHF05_02175"
     CDS             <1..414
                     /locus_tag="VHF05_02175"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVS79761.1"
                     /translation="VGAAGKILDKLLADVGIDRADVYITNIVKDRPPQNRDPQPAEIA
                     SYGPFLDRQIDIIRPKILATLGRFSMVYIMTKFGLTEELGPISKIHGLTFDAKASYGN
                     VKIVPLYHPAVAVYNSSTLDTLKEDFKVLPKLIKE"
     gene            complement(460..2439)
                     /locus_tag="VHF05_02180"
     CDS             complement(460..2439)
                     /locus_tag="VHF05_02180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVS79762.1"
                     /translation="MKNKTKRSVSSWGFDPKNLDTSVRPQNDFFHFAAGGWIKRHPIP
                     PTKAKWGTFYVLREDGLKKLRALIEEIADSSHAKGTEGQIIRDFYLSGMDTKTRNKLG
                     LKPLEPFFRKIDAIKNGQNIIDFIAYLHKAGFSLLWGTHVGQDDKDSEHYTVFLAQSG
                     LTLPDRDFYLKKDPRSETIRDEFLKYIEKMLVIAGEKPAQAREDARVILELETKLARF
                     SMSNVDTRDIEKVYNRKSVQELGKLAPAVEWRKFFETVGIKNARSLVVLQPEFISKAT
                     ALLAKTPLDTWKKYLRWHVLNGCSPLLSTKFISIRFQFFGKVLMGSQKPEPVWKRVIG
                     ALETYIGEAVGKEYVKRHFPPEAKKKIDELVDNLFAAYEERLKNIDWMSPATKKKALA
                     KLAAISRKLGYPSKWKSYKGLDIRPDEYFQNAMRALRLERRREIGRYGKKVDRTEWFM
                     TPQTVNAYYSPNMNEIAFPAGILQPPFFDPDADAAINYGAIGSVIGHEITHGFDDQGS
                     KFDGRGNFKNWWTADDRKRFDKKANVLVKQFNGYKVGDINVNGKLTLGENIADLGGAV
                     MAYYAYMRHLEREGRRDIAGFTPEQRFFLGLTLVESGHQRPEAARTQVMTDPHSPAVF
                     RINGPTSNMTEFYEAFGVTKEDRLYRDPKDRAKIW"
     gene            2576..2863
                     /locus_tag="VHF05_02185"
     CDS             2576..2863
                     /locus_tag="VHF05_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVS79763.1"
                     /translation="MNKRTKTAKQMERHLKGLGNHYRIDILLHVSGKPGLTIDQLAEG
                     VDGNYKTISQHVRYLVHAGLLEVSHESTFAHIKLSPYGGACVEFLKKFQSL"
     gene            complement(3037..3387)
                     /locus_tag="VHF05_02190"
     CDS             complement(3037..3387)
                     /locus_tag="VHF05_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79764.1"
                     /translation="MGLYYSFKEKARRKDFCRTLEREIKRIFISRGLNAEMTIEGLDE
                     EITELTFEANQEDMTVIKIKVFWHSNRCDYFNLTVGEYEKIISGTFLLDGRKMFRLIQ
                     NFVDNRGRAEELLM"
     gene            4042..5931
                     /locus_tag="VHF05_02195"
     CDS             4042..5931
                     /locus_tag="VHF05_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HVS79765.1"
                     /translation="MKTADVRAKYLEFFKKQGHQIIPSASLVPENDPTTLFTGSGMQP
                     LVPYLLGQKHPLGDRLANSQKSFRSGDIEDVGDNRHTTFFEMLGNWSLGSYFKAEQLP
                     WLFAFLTDEVGIDPKRLYVTAFLGDETNGIPKDTEAADIWKKLFAEKGIEAKDIEIGS
                     EENGYKIGMQGGRIFYYDAKKNWWSRSGTPDKMPAGEPGGPDSEVFYCFEEIEHDTKF
                     GAECHPNCDCGRFLEIGNSVFMEYKRTESGFEKLPQRNVDFGGGLERVAAVSENNSDV
                     FKIDIFKGTIETLEKLSGKSYSDPAFIAAFRVVADHLRSSIFLIGDGVVPSNSERGYF
                     VRRLLRRAVRYWDKLGITEGGLSTLVDLILEFYKDAYPETFSAKETIKQEIEKEETKF
                     RRTLKDGLRQFEKIAVADISGTDAFNLLQSYGFPLELTTELAKERGISVDVESFKEEF
                     KKHQDLSRAGSEKKFKGGLGDTSEATVKLHTAHHLLLKALQIVLGEHVHQRGSNITPE
                     RLRIDFMNPEKVTPEKLAEAERIVNEKIAEDLPVIRSEMPREEAEKLHAEHEFGAKYP
                     DVVSVYSVGPKGATKEDPKFSQAYSIEFCGGPHVEHTGVLGTFKIQKEEAVSAGVRRI
                     KAVLE"
     gene            5932..6402
                     /locus_tag="VHF05_02200"
     CDS             5932..6402
                     /locus_tag="VHF05_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HVS79766.1"
                     /translation="MEKSLILFNKQEIGKGVRANRKYAAGEHILVFSGPLLDRDELPE
                     IITPEDDRYMQIGIRTFIGSSRRVDDFVNHSCDPNSGAKFHDNRVELVALRDIEAGEE
                     VTWDYSTTMFEDDWEIECLCGAYNCRGRIREFRYLPDDIKRKYISFGIVPEYNM"
     gene            6424..6780
                     /locus_tag="VHF05_02205"
     CDS             6424..6780
                     /locus_tag="VHF05_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HVS79767.1"
                     /translation="MKLLLKRAYEPAGKEDGTRVLVDLLWPRGVSKEKAKIDIWAKDV
                     TPTPKLRTWFGHKPERWKEFSKKYKEELKGKKAELKALMGEIKKLGPKATLVYGAKDT
                     DHTHAIILKEALEKIK"
     gene            7257..8468
                     /locus_tag="VHF05_02210"
     CDS             7257..8468
                     /locus_tag="VHF05_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79768.1"
                     /translation="MFESFANPGTSRQEDACVLLDIGNGSVGGSIVLFARSQKPKIVY
                     SVRYPIHIPEKPSSAHLLEKMTRLLEDVVKDLTHEGSRHLDFKLLNYKDIKHVFCIFS
                     SPWYASKTKIFRIEHREPVPVTDEFVKYLLDKEAESFEKSIGGTGTGTLKFIEQKVIR
                     TRLNGYDTKAPHLQAARKIELGVFMSMVPQAVLDRVSAIVSRYFNARHIEFNSFALMS
                     FSLIEDLFPGSRSYMFLDVTSEVTELALVRNNLITDTISIPSGKNLIMRAIARRYGVS
                     PDIAATYMKLYANGHAEKSLKKGIEAAIETARAAWTAQFEKAVKEVSKGHAAPSRVFL
                     TADDDVAIVFKDFIGETAHVVEGRPEYFSVTPLNKGILAHFCTFGRGSDEDSFLAIES
                     VYFNKIFHLEA"
     gene            8526..9941
                     /locus_tag="VHF05_02215"
     CDS             8526..9941
                     /locus_tag="VHF05_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79769.1"
                     /translation="MAKRTFQDIVPPEKRTIRNIPIPEGRKPRLDIEPPRKRAEINEP
                     LEAPKPTRRSRPEPENEPAWVPPQRPRTSSSGRKGSRWGLWIVVVVVLLVLAFAISSL
                     LADATVKVTERNAAAQVSGTFTASRTGEDDTIPFDIIKVSRDESKSISADAAAAPAAS
                     STAEKSPATKASGQIVIYNNQSSSSQTLIANTRFQTSDGLIYRISKAVAVPGTTIKDG
                     QKVPGSVEVTVTADQAGAKYDIGLVDFKIPGFKGDPRYDTIYARSKTPMTGGADEGSA
                     PGVSSDTVSQTRDEIDYDLKQALLSDAKAQIPQNFTTIDSGLIYSFTHDAPASDPANP
                     DSAKITEHGTLYAVIVNETSLGQAVADRFLPVPGGGDVVVSNLPDLNFTISSTTPFDP
                     EKDTQFDFTLNGTAQLVWTYDQAALKKDLEGIGKNEVNSILAKYPSIKSADVVVRPFW
                     NRNLPKDPQKITITNVVSQSQ"
     gene            10157..10375
                     /gene="infA"
                     /locus_tag="VHF05_02220"
     CDS             10157..10375
                     /gene="infA"
                     /locus_tag="VHF05_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVS79770.1"
                     /translation="MDTKKEKEGALGTVIEALPNTLFRVKLDDSEEEMLAYLSGKMRM
                     HRIRVLIGDKVRVDLDPYGGRGRISKRL"
     gene            10405..10521
                     /gene="rpmJ"
                     /locus_tag="VHF05_02225"
     CDS             10405..10521
                     /gene="rpmJ"
                     /locus_tag="VHF05_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVS79771.1"
                     /translation="MKVKASVKKRCPKCQIVRRKGYLYVVCKANPRHKQRQG"
     gene            10563..10940
                     /gene="rpsM"
                     /locus_tag="VHF05_02230"
     CDS             10563..10940
                     /gene="rpsM"
                     /locus_tag="VHF05_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVS79772.1"
                     /translation="MRILGITIPDEKRLEIGLTVLFGVGRSRAREILTQAKVDFGKKS
                     KDLSLDEENAIRRIVEGYKIEGDLKREVSGNIKRLKDIKSYRGSRHARHLPSRGQRSK
                     TNARTLKGPKKTMGTGRKKVEKK"
     gene            11014..11445
                     /gene="rpsK"
                     /locus_tag="VHF05_02235"
     CDS             11014..11445
                     /gene="rpsK"
                     /locus_tag="VHF05_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVS79773.1"
                     /translation="MGKKRIISKGEEAGAEESKSHVQAVSKKKAEVGILHVQSTYNNT
                     KVTLSDLRGNTLAWSSSGSLSFKGAKKGTPFAAAKVGEVVGDRAQTIGIQDVHVVVKG
                     VGSGRESSIRGFVSKGFNILSIVDRTPVPFNGPKSKKPRRV"
     gene            11482..12096
                     /gene="rpsD"
                     /locus_tag="VHF05_02240"
     CDS             11482..12096
                     /gene="rpsD"
                     /locus_tag="VHF05_02240"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVS79774.1"
                     /translation="MKVGPKYKIARRLGAAVFEKTQTSKYAASESKRGAVRTQRRGGA
                     KSDFGLQMLEKQKARYSYLVTEKQFANYVKAALASKGTENVPTLFKLLESRLDNVVLR
                     SGLAHTRPLARQLVSHGHIMVNGRRVTIPSFQVKMGDVVSIRPGSRSSKVFSALDERL
                     KAISIPAWLKLDADKGEVTVQGAPQMQNANLLFDLNAVFEFYSR"
     gene            12168..13139
                     /locus_tag="VHF05_02245"
     CDS             12168..13139
                     /locus_tag="VHF05_02245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVS79775.1"
                     /translation="MSDLSIVLPSKPKIVSEDSFSGVYEIDGLHPGYGNTLGNSLRRV
                     ILSSLPGAAITTVKIAGISHEFSVIEGVKEDAITLLLNLKRIRIKMITDEPQTIRLKQ
                     KGAKEVVAGDIEVPGQVEILNPELVIAHMTDKNSDLDIEMTVERGLGYVSKDVLQKNR
                     VDIGTIALDASFTPIRRVNYEVENMRVGDRTDFNRLRISIETDGTLTPKEALEKSIEI
                     MINQLKSVIGFKEEEPEMHSESGDADEAEIGDEKGEIDPEVLKTRTESIGLSARTLNA
                     LTNANIRTVGGLARKREKDILEIEGLGSKGVQEVKKALSNLGITLKA"
     gene            13166..13513
                     /gene="rplQ"
                     /locus_tag="VHF05_02250"
     CDS             13166..13513
                     /gene="rplQ"
                     /locus_tag="VHF05_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVS79776.1"
                     /translation="MRHHNANRKFGLKANKRNALMKSLARSLVLKEKITTTEAKAKEL
                     RPYVEKLVTAGRENNVAARRSLSAKVGAEGGKKLVEVLGPKYKERKGGYTRITKLGSR
                     NNDASPMAQIEFV"
     gene            13519..13863
                     /gene="rplM"
                     /locus_tag="VHF05_02255"
     CDS             13519..13863
                     /gene="rplM"
                     /locus_tag="VHF05_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVS79777.1"
                     /translation="MKHVIDATNKKVGRVATQAATLLMGKNTAAFVRNAAPEVEVHII
                     NTSKASIDEKKAVQKKYVRFSGYPGGLKETPLRRMAEEKGYSEVFRKAVKGMLPKNKL
                     QAVMLKNLTISE"
     gene            13868..14308
                     /gene="rpsI"
                     /locus_tag="VHF05_02260"
     CDS             13868..14308
                     /gene="rpsI"
                     /locus_tag="VHF05_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVS79778.1"
                     /translation="MAEATKTSARSAAADKKEKYIEAVGRRKTAVARVRLVASSKMAY
                     TVNGKELEIYFPTDELQRIVRDPFTAAKDASKFKITAKIVGGGIHSQAEALRHGIARA
                     LIKSDMELRKKIKKAGFLERDQRAKERRKFGLKKARKAPQWSKR"
     gene            14630..>14845
                     /locus_tag="VHF05_02265"
     CDS             14630..>14845
                     /locus_tag="VHF05_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79779.1"
                     /translation="MNDENNTHDENFDEEASEETASEEADDDVAFEPEDNETPLSSFG
                     KSKGDALASLKEKLKASEAKAAEYLTDL"
BASE COUNT         3642 a         4013 c         4028 g         3162 t
ORIGIN      
        1 gtcggcgcgg cgggaaagat tctcgacaag ctattggccg atgtgggcat cgatcgcgcg
       61 gacgtctaca ttaccaatat cgtcaaggat cggccgccgc agaatcgcga tcctcagcct
      121 gcggaaattg caagctatgg cccgtttctt gatcgccaga tcgacatcat ccggccgaag
      181 attctcgcaa cgctcgggcg cttctcgatg gtgtacatca tgacgaagtt cggcctcacg
      241 gaggagctcg ggccgatcag caagatccac ggccttacct tcgacgcgaa agcttcatac
      301 gggaatgtga aaatcgtgcc gctctaccac cccgcggtcg ccgtctacaa ttcatcgaca
      361 ctcgatacgc tcaaggagga tttcaaagtt ttgccaaagc ttatcaaaga atgatctcga
      421 agctcctaac aaaagcggcg cgatatcgcg ccgttttcgt taccagattt tcgcccggtc
      481 tttgggatcg cggtagagtc tgtcttcttt cgtcactccg aacgcttcgt agaactcggt
      541 catattcgat gtcggtccgt tgatgcggaa taccgcggga gaatgcgggt cggtcatgac
      601 ttgggtccgc gccgcttcgg gccgctgatg gccgctctcg acgagcgtga gtccgaggaa
      661 gaatcgctgt tcgggcgtga agccggcgat atccctgcgg ccttcgcgct cgagatgccg
      721 catgtatgcg tagtatgcca tcactgcgcc gccgagatcg gcgatgtttt cgccgagcgt
      781 cagcttgccg tttacgttga tatcgccgac cttgtagccg ttgaactgct tcacgagcac
      841 attcgctttc ttgtcgaacc gctttcggtc atctgccgtc caccaatttt tgaagttgcc
      901 gcgtccgtcg aatttcgagc cctggtcgtc gaaaccgtgg gtgatctcat ggccgataac
      961 cgatccgatc gctccgtaat tgatcgccgc atccgcgtcc ggatcgaaaa acggcggctg
     1021 gagtattccg gcagggaagg cgatctcgtt catgttcggg ctgtagtacg cgttcaccgt
     1081 ttgcggcgtc atgaaccatt cggtcctgtc tacctttttt ccgtagcgac cgatttcgcg
     1141 ccttcgctcg agcctcagtg cccgcattgc gttttggaaa tattcgtcgg ggcgtatgtc
     1201 gagccctttg tacgacttcc attttgacgg atatccgagc ttgcggctga tcgcggcgag
     1261 cttcgcaaga gcctttttct ttgtcgcggg ggacatccag tcgatattct tgagccgttc
     1321 ctcgtatgcc gcaaagagat tgtcgacaag ttcgtcgatc ttctttttcg cttcgggcgg
     1381 gaagtggcgc ttcacgtatt ccttgccgac cgcctcgccg atataggttt cgagcgcgcc
     1441 gatgacccgc ttccagaccg gctccggttt ctggctgccc atgaggactt tgccgaaaaa
     1501 ttgaaatcgt atgctgatga attttgtcga gaggagcggc gagcagccgt tcagtacgtg
     1561 ccatcggagg tattttttcc acgtatcgag aggagtcttc gcaaggagcg ccgtcgcttt
     1621 ggatatgaat tccggctgga gcaccacgag agatcgcgca ttcttgatgc cgacggtttc
     1681 gaaaaatttc ctccattcga ctgccggggc gagcttcccg agctcctgaa cgcttttccg
     1741 gttgtatacc ttctcgatat ctctcgtgtc gacattgctc atcgaaaaac gggcgagctt
     1801 ggtctctagc tcgaggatga cgcgcgcgtc ttcgcgcgcc tgtgcgggct tctcgccggc
     1861 gatgacgagc atcttctcga tatatttgag gaattcgtcg cggatcgttt cggaacgcgg
     1921 gtctttcttg aggtagaaat cgcggtcggg aagggtgagg ccgctctgag caaggaacac
     1981 cgtgtaatgc tcggaatcct tgtcgtcctg gccgacgtgc gttccccata agagcgagaa
     2041 gcccgcctta tggaggtagg cgatgaaatc gatgatgttc tgcccgttct tgatcgcatc
     2101 gattttcctg aagaatggtt cgaggggctt aagtcccagc ttattgcgag tcttcgtgtc
     2161 catgccggag agatagaaat cgcggatgat ctggccttcg gtgcctttgg catggctgga
     2221 gtcggcaatc tcctcaataa gggcgcggag cttcttgagc ccgtcctcac ggagaacgta
     2281 gaacgtgccc catttcgcct tggtcggagg gatcgggtgc cttttgatcc agccgccggc
     2341 cgcaaaatgg aaaaagtcgt tttgcggacg gaccgatgtg tcgagattct taggatcgaa
     2401 tccccaggaa gaaacggagc gtttggtttt gtttttcatg cctgcagttt aacccaactt
     2461 catgaggcag aaaagttcga ttatgccccg gaaggcccgc gctcattgag gcgaaaccat
     2521 gccataattc tgcagaatta tggcatgtcg cccgcgcaga gcgtatactt attgcatgaa
     2581 taaacgaacc aaaaccgcga agcagatgga acgccatctc aaaggcctcg gcaatcatta
     2641 ccgcatcgat attctgctcc atgtgagcgg gaagccgggg ttaactatcg atcagcttgc
     2701 ggagggagtc gacggaaact acaaaacgat ttcacagcac gtcagatatc tggtgcatgc
     2761 gggcctactc gaggtgtcgc acgaaagtac gttcgcgcac attaagcttt cgccctatgg
     2821 cggagcatgc gtcgaatttc tcaaaaaatt ccagtcgctc taactgcacc tgtttcgaac
     2881 ttcgatcgtg cggtttcaaa catgctataa ttctgcagaa ttatagcatg tcgcagataa
     2941 tacagttgaa gcgaaaaagt ctgaaaaaat tttatatatt agagaataat aaaaagcagg
     3001 cagaggatta aatccctgcc tgcttactct tggaactcac atcagcagct cctcagctcg
     3061 tcctctgttg tcaacgaaat tctgaatcaa tcgaaacatt tttctgccat caagcaaaaa
     3121 agttcctgaa atgattttct catactcacc gacggtcaaa ttaaaataat cgcatcggtt
     3181 tgaatgccaa aataccttta tcttgatgac cgtcatgtct tcctggttgg cctcgaaagt
     3241 caactcagtg atctcttcgt cgagaccttc gatcgtcatt tctgcgttca agccgcggct
     3301 gatgaaaatg cgctttattt cccgctccaa tgttctgcaa aaatccttcc tccgcgcttt
     3361 ttctttgaag gaatagtaaa gtcccattcc aataattcca attaccagtc caaaaagcaa
     3421 actgtaaatt tccactccta aatttcctcc gattcaaaat tacaggttat tttcatattt
     3481 gtcaaaattc ttcttatcag atgaaattat tatgctataa ttctgcagaa tataggcatg
     3541 tcgcgcgcgg cactggagaa acgaggaata aaaaagcggg caggggatga acccgtgccc
     3601 ggtgtgcctc tagcggacaa ttgtaacctc gagtttgacc ggaaattcgc tgccgacagc
     3661 atcgcagtat attgcgtacc acgctttgtc ggacgatcga tatacgatct ctcgatgaga
     3721 gaatacgata tgccgatcat tcaaaggtat ggaaatgaga atcggcttac ttagacccaa
     3781 tattacctgc tgggggcccg aacgcactgt gagtgaggta acctcggccg gcatctccgt
     3841 tggcagctta ggagcgcgcg gtccggtttg cctgatcact actctgaatg gaaaactgtt
     3901 atccagcggc acgcttgctt cttcaagaag cgaatccata ggaataaatt cctcctttct
     3961 ttccttctgc gcacaaaata taatttattc atacgattgt caaataccag caaatcgcat
     4021 ctttatgata gattgttatt tatgaaaacc gctgacgtac gggctaaata cttggaattt
     4081 ttcaagaaac agggacacca gatcatcccc tcggcatcgc tcgtgcccga gaacgatccg
     4141 acgacgcttt tcaccggttc cggcatgcag ccgctcgttc cgtatctcct gggccagaag
     4201 catccgctgg gcgatcgtct cgcgaattcg cagaagtcgt tccgctcggg cgatatcgag
     4261 gatgtcggcg acaaccgcca tacgaccttc ttcgaaatgc tcggcaactg gtcgctcggc
     4321 tcgtatttca aggcggagca actgccgtgg cttttcgcgt tcctcaccga cgaggtcggg
     4381 atcgatccga agcgtctcta cgtgactgcg ttcttgggcg atgaaacgaa cggaattccg
     4441 aaggatacag aagcggccga tatctggaag aagctttttg cggagaaggg aatcgaggcc
     4501 aaagatatcg agatcgggag cgaggagaac ggctacaaga tcggcatgca gggcggccgc
     4561 atcttctact acgacgccaa gaagaactgg tggagccgct cgggcactcc cgacaagatg
     4621 ccagccggag agccgggagg accggactcc gaagtcttct actgcttcga ggagatcgag
     4681 catgatacga aattcggcgc cgagtgccat ccgaactgcg actgcgggcg ctttctcgag
     4741 atcggcaact ccgtcttcat ggaatacaag cgcacggaga gcggcttcga gaagctgccc
     4801 cagcgcaatg tcgatttcgg cggcggcctc gagcgcgtcg ctgcggtttc cgagaacaat
     4861 tccgacgtct tcaaaatcga tattttcaaa ggaacgatcg agacgctcga gaagcttagc
     4921 ggcaaatcat attccgaccc ggcttttatc gcggcattcc gcgtcgtagc agaccatctc
     4981 cgctcctcga tctttctcat cggcgacggt gttgtgccta gtaacagcga gcgcggctac
     5041 ttcgtgcgcc gcctgctccg ccgcgccgtg cgctactggg acaagctcgg cataaccgaa
     5101 gggggactct cgacccttgt cgatctgatc cttgagttct acaaggatgc gtatcccgag
     5161 acattcagcg cgaaggaaac gataaaacag gagatagaga aagaggaaac gaaattccgc
     5221 aggacgctta aagacggcct gaggcagttt gagaagattg cggtcgcgga catttcggga
     5281 acggacgcat tcaatctcct ccagtcgtac ggctttccgc tcgaactcac gaccgagctc
     5341 gcgaaggagc gcggcatctc ggtcgacgtc gagagcttca aggaagaatt caagaagcac
     5401 caggacctct cccgcgccgg ctccgagaag aagttcaaag gcggcttggg cgatacgagc
     5461 gaggcgacag tgaagctcca caccgcgcac catttgctac tcaaggcttt gcaaatcgtc
     5521 ctcggtgagc atgtgcacca gcgcggctcg aacatcacgc ccgagcgcct acgcatcgat
     5581 tttatgaatc ccgagaaggt aactcccgag aagctcgccg aagccgagcg gatcgtcaat
     5641 gaaaaaatcg cggaagacct gccggtgatc cgcagcgaaa tgccccgcga agaagctgag
     5701 aagctccatg cggagcacga gttcggagcc aagtatccgg acgtcgtttc cgtctactcg
     5761 gtcggcccca agggcgcgac aaaggaagac ccgaagttct cgcaggctta ctctatagaa
     5821 ttctgcggcg gtccgcatgt cgaacacacc ggtgtcttag gcacattcaa aatccagaaa
     5881 gaagaagcgg tttccgcagg agtgagaagg attaaagcgg ttttagaata aatggaaaag
     5941 agtttaatac tcttcaataa gcaggagatt ggaaagggtg ttcgcgcgaa tcgaaaatat
     6001 gccgcaggcg agcatatttt agttttttcc ggaccgctgc ttgatcgcga cgaattgccc
     6061 gaaattatta ccccggaaga tgacaggtat atgcagattg gtattcgcac ctttattggt
     6121 tcttccagga gagtcgatga ttttgtgaac cattcgtgtg acccgaattc cggagctaaa
     6181 tttcatgaca atagagtgga gcttgtcgca ttgagggata tagaagcggg tgaggaagtt
     6241 acctgggatt attccacgac gatgtttgag gatgattggg agattgaatg cctgtgtggc
     6301 gcttataatt gcaggggacg aatcagggaa ttcagatatt tgcctgatga tatcaagcgg
     6361 aaatatattt catttggaat cgtgccggaa tataatatgt aagtattata aatcgcaaaa
     6421 tcaatgaaac tactgcttaa gcgtgcatat gagccggcgg ggaaggagga cgggacgaga
     6481 gtgctcgtgg atttgctctg gccgcgcggc gtttccaagg agaaggcgaa gatcgatatc
     6541 tgggccaagg acgtgacgcc tacgccgaag cttcgcacct ggttcggcca caagccggaa
     6601 cggtggaagg aattctccaa aaaatacaag gaagagctca agggcaagaa agctgagctc
     6661 aaggcgctta tgggcgaaat aaagaagctc ggaccgaagg ccactcttgt ctacggcgcg
     6721 aaagataccg atcataccca tgcgattatc ctcaaggagg cgcttgaaaa aataaaataa
     6781 gaaaaatgtc ctccagccgt aagctggagg actagttttt aatggcaagc ataatgatct
     6841 tcgtgttccc tgattttcaa cgtgagttca ttgtcgtgct tccatttcga agtcgtgaga
     6901 acgatgcctg tatgcgagat ttcaatgatg cgagatttac atattttctc agtgcaacct
     6961 tcacgagtgc atgtaaacag gccatgttgt aaatattcaa tttcatcatc gtcagaatca
     7021 ttctccacgg tctggagaat agacggttcc gaccaggcat ggatgtcgca tgcgcagaga
     7081 atgaatcgcc aagctcttcg cagtaatccc atatgacctc ctcaaaaagt gttccagtga
     7141 aatagaagca ccttaccgcc gcggctgtca atcgccgcgc aacagctcca cttatccact
     7201 tatgcgccat ggtcgcaact ttggaatttt ggttttcggc ttgtataata aatgcaatgt
     7261 tcgaatcatt tgcgaacccg ggaacttcgc ggcaggaaga cgcatgcgtc ctcctcgaca
     7321 ttggcaatgg aagcgtcgga ggctccatcg tgctcttcgc ccgctcgcag aagccgaaga
     7381 tcgtctactc ggtgcggtat ccgatccaca ttcccgaaaa gccgagctcc gcgcatctcc
     7441 tcgagaaaat gacgcgcctt ctcgaagacg tggtgaaaga cctcacgcat gagggaagcc
     7501 ggcatctcga tttcaagctc ctcaattaca aggatatcaa gcacgtcttc tgcatcttct
     7561 cgtcgccctg gtacgcatcg aagacgaaga tcttccgcat cgagcaccgc gagccggtgc
     7621 cggtgaccga cgaattcgtg aaatatctcc tcgacaagga ggctgaatcg ttcgagaagt
     7681 cgatcggcgg cacgggaacc ggcacgctca agttcatcga gcagaaggtc atccgcacgc
     7741 gcttgaacgg ctacgacacc aaggcgccgc atctccaggc ggcccgcaag atcgagctcg
     7801 gcgtattcat gagcatggtg ccgcaggccg tgctcgacag ggtgagcgcg atcgtcagcc
     7861 gctatttcaa cgcgcgccac atcgaattca actccttcgc gctcatgtcg ttcagtctga
     7921 tcgaggacct ctttcccgga agcaggagct atatgtttct cgacgtgacg tccgaagtga
     7981 cggagctcgc gcttgtccgc aataatctca tcactgacac gatttccatt ccgtcgggaa
     8041 agaacctcat catgcgggcg atcgcgcgcc gttatggcgt atcgcccgac atcgccgcaa
     8101 catatatgaa gctctatgcg aacggccacg ctgaaaagtc gcttaagaag gggatcgaag
     8161 cggcgatcga gacggcgcgg gctgcctgga cggcgcagtt cgaaaaggcg gtgaaggaag
     8221 tttccaaggg ccatgcggcg ccttcgcgcg tctttctcac cgccgacgac gacgtagcga
     8281 tagtcttcaa ggatttcatc ggagagactg cgcatgtcgt ggagggcagg cccgagtatt
     8341 tcagcgttac gccgctcaac aagggaatcc tcgcccactt ctgcaccttc gggcggggaa
     8401 gcgacgaaga ctcgttcctc gcgatcgaga gcgtctattt caataagata ttccacctcg
     8461 aggcgtgaac gtcgcggttt acaaataaaa ttcagttgtt ttacaataat taaattaatc
     8521 ggcctatggc aaaacgaact ttccaggata tcgtcccgcc cgaaaagcgc accatcagga
     8581 acattcccat tccggaaggc cggaagccgc gtctcgacat cgagccgccg cgcaagcgcg
     8641 ccgaaataaa cgaacctctc gaggcgccga agccgacgcg ccgcagcagg cccgagcccg
     8701 agaacgagcc tgcctgggtt ccgccgcaga ggccgcgcac ttcttcttcc ggccgcaagg
     8761 gctcccgctg gggtctgtgg atcgtggtgg tcgtggtgct tctcgtcctc gcattcgcca
     8821 tttcgtctct ccttgccgat gcgaccgtca aggtgaccga gcgcaacgcg gccgcccagg
     8881 tttccggcac gtttaccgcg agccgtaccg gcgaagatga cactattccc ttcgacatca
     8941 tcaaagtgag ccgcgatgag agcaagtcga tctctgcgga tgccgctgct gcgcctgctg
     9001 catcgtccac cgccgagaag agccccgcca ccaaggcatc ggggcagatc gtcatctaca
     9061 acaaccagag ctcgagctcg cagactctta tcgccaacac ccgtttccaa acgtcggacg
     9121 gcctcattta tcgcatctcg aaggcggtgg cggtgccggg cacgacgatc aaggacggcc
     9181 agaaggtgcc gggaagcgtc gaggtaacgg ttaccgccga ccaggcaggc gcgaagtacg
     9241 atatcggcct cgtcgacttc aagattccgg gcttcaaggg cgatcctcgc tatgacacca
     9301 tctatgcgcg ctccaagacc ccgatgacgg gcggcgccga cgagggcagc gcgcccggcg
     9361 tatcgagcga cacggtaagc cagacccgcg acgagatcga ctacgatctg aagcaggcgc
     9421 tcttgagcga cgccaaggct cagatcccgc agaacttcac tacgatcgac agcggcctta
     9481 tctattcgtt cacgcatgac gcgcccgcgt ccgacccggc caatcctgat tccgcgaaga
     9541 ttaccgagca cggcaccctc tatgccgtca tcgtgaacga gacgagcctt ggccaggcgg
     9601 tcgccgacag gttccttccg gtgccgggag gcggagatgt ggtcgtctcg aatctccctg
     9661 acctcaactt cacaatttcg agcaccacgc cgttcgatcc ggagaaggac acccagttcg
     9721 acttcaccct gaatggcacg gcccaactcg tctggacgta tgaccaggcg gccttgaaga
     9781 aggacctcga gggcatcggc aaaaacgagg tgaactccat tctggccaag tatccgtcca
     9841 tcaaatcggc cgatgtggtg gtccggccgt tctggaaccg caacttgcca aaagacccgc
     9901 aaaaaattac tattactaat gtcgtatcgc aaagccaata agcttttcgg cctacggcac
     9961 acagatcgac cctgttccaa aaggaagagg gttgaccccg ttagaaatct tcggcaagga
    10021 acttgcacgc aaggattgag ggggagatat gttataatat tatagtgtaa tatcggaact
    10081 cagctcccgt tgaatttcta acggggttga ctaatttgtg catattctgt tagtatattt
    10141 ttcattgtaa aaattgatgg ataccaagaa ggaaaaagag ggtgcgttgg ggacggtcat
    10201 agaggcgctt ccaaacacgc ttttccgcgt caagctggat gattccgaag aggagatgct
    10261 cgcgtatctg tcagggaaga tgagaatgca ccggatccgc gtccttatcg gcgataaggt
    10321 gagggtcgat ctcgacccgt acggggggag ggggaggatc tcgaagcggc tttaatcgat
    10381 agcaagaatt tagatttaag agagatgaaa gtaaaagcgt cagtcaaaaa gcgctgcccg
    10441 aaatgccaga tagtccgcag gaagggctac ctgtatgtcg tgtgcaaggc gaatccccgc
    10501 cataagcaga ggcagggcta ggagccgtat caataattag caacgttacc gataagaaaa
    10561 caatgcgtat tcttggaatc acaataccgg acgaaaagag gcttgagatc ggcttgaccg
    10621 ttctcttcgg cgtcggacgc tcgcgcgccc gtgaaatcct cactcaggcg aaggtcgact
    10681 tcggcaagaa gtcgaaagat ctctcgctcg acgaggaaaa cgccatccgc cgcattgtag
    10741 aaggatacaa gattgaaggc gatttgaagc gcgaggtctc gggcaacatc aagaggctca
    10801 aggacatcaa gagctaccgc ggaagccgcc atgcccgcca ccttccgtcc cgtggacagc
    10861 gctccaagac caacgcccgt acgctcaagg gtcccaagaa gaccatgggc accggtcgca
    10921 agaaggtaga gaagaagtaa gcctgaggtc tgccgatgga cgtatcccgt cccggcggga
    10981 tccagggctt caaaaataaa tcacatcatt cctatgggta agaaacgaat tatttcaaaa
    11041 ggcgaagaag caggggcaga ggaatcgaag tcccatgttc aggctgtctc caagaagaag
    11101 gccgaggtag gcattctcca cgtccagtcg acttacaaca acaccaaagt tacgctttcc
    11161 gatctccgcg gcaacacgct cgcgtggtcg tcgtcgggaa gcctcagctt caagggcgcc
    11221 aagaaaggca cccccttcgc tgccgccaag gtcggcgaag tcgtcggcga cagggctcag
    11281 accatcggca tccaggacgt ccatgtagtc gtgaagggcg tcggatcggg ccgcgagtca
    11341 tcgatccgcg gattcgtttc gaagggcttc aacatccttt ccatcgtcga tcggacgccg
    11401 gtgccgttca acggaccgaa gtcgaagaag ccccgccgcg tgtaattcca aatatttgat
    11461 ttttaatctt ttgattttcg tatgaaagta ggaccaaaat acaaaatagc gagaaggctc
    11521 ggcgcggctg tcttcgagaa gacgcagacc tccaaatacg cggcttccga atcgaagaga
    11581 ggcgcggtca ggacccagcg ccgcggcggc gccaagtcgg acttcggact tcagatgctc
    11641 gagaagcaga aggcccgcta ctcctatctc gtcactgaga agcagttcgc gaactacgtg
    11701 aaggccgcgc tcgcatccaa gggcaccgaa aacgtaccga cgctcttcaa gctccttgag
    11761 agccgcctcg acaatgtcgt cctccgcagc ggtctcgccc acacccgccc gctcgcgcgc
    11821 cagctcgtat cgcatggcca tatcatggta aacggccgcc gcgtcactat tccctccttc
    11881 caagtgaaga tgggcgacgt cgtttccatc cgccccggct cccgctcctc gaaagtcttc
    11941 tcagcgctcg acgagcgcct gaaggcgatc tcgattcccg catggctcaa gctcgatgcc
    12001 gataaggggg aagtaaccgt ccagggggcg ccccagatgc aaaacgcgaa tcttctgttc
    12061 gatctgaacg ccgtcttcga gttctacagc aggtaagcag gtagtatatg ttgcaaatac
    12121 gcaacatttg atgcataatt tatcagttaa tatttaataa aacacttatg tcggatctca
    12181 gcattgtctt gccctcaaag ccgaagatcg tctccgagga cagcttctcg ggcgtctacg
    12241 aaatcgacgg ccttcatccg ggctacggca ataccctcgg caacagcctc cggagggtca
    12301 tcctttcgtc tcttccggga gctgctatca cgacggtgaa gatcgctggc atcagccacg
    12361 agttttccgt catcgaaggc gtgaaggagg acgcgatcac gcttctcttg aacctcaagc
    12421 gcatccgcat caaaatgatc acggatgagc cccagacgat ccgcctcaag cagaagggcg
    12481 cgaaggaagt cgtcgcgggc gatatcgagg ttcctggtca ggttgaaatc ctgaatcccg
    12541 agctcgtcat cgcacacatg accgacaaaa actccgatct cgacatcgaa atgaccgtcg
    12601 agcgcggctt gggctacgtt tccaaggacg tgcttcagaa gaatcgcgtc gatatcggca
    12661 cgatcgctct cgatgcgagc tttacgccga tccgccgcgt aaactacgaa gtcgagaaca
    12721 tgcgcgtcgg cgacaggacc gacttcaacc gccttcgcat ctccatcgaa accgacggca
    12781 cgcttactcc gaaggaggct ctcgagaaat cgatcgagat catgatcaac cagctcaaat
    12841 ccgtcatcgg attcaaggag gaagagcctg aaatgcactc agaatcgggc gatgcggacg
    12901 aagcggagat cggcgacgag aaaggggaga tcgacccgga ggtcttgaag acccgcaccg
    12961 aatcgatcgg cctttcggct cggactttga acgctctcac caatgccaac atcaggacgg
    13021 tcggcggctt ggcaagaaag cgcgaaaagg atatcctgga gatcgaaggt ttgggctcca
    13081 agggcgtcca ggaggtcaaa aaggcccttt cgaacctcgg tataacgctc aaggcttaat
    13141 tttgactttt gaattttgat ttaacatgag gcaccacaac gcaaacagaa aattcgggct
    13201 taaggccaac aagcgcaacg ctttaatgaa gtcgttggct cgttcgctcg tgctcaagga
    13261 gaagatcact acgaccgagg cgaaggccaa ggagcttcgc ccgtacgtcg agaagcttgt
    13321 gactgccggc agggagaata acgtagcggc acgccgctcg ctttccgcaa aggtgggcgc
    13381 cgaaggcggc aagaagctcg tcgaagtcct gggccccaag tacaaggagc gcaagggcgg
    13441 ctacaccagg atcaccaagc tcggctcccg caacaatgat gcgagcccga tggcccagat
    13501 cgaattcgta taaatacaat gaagcacgta attgacgcaa caaacaagaa ggtaggaagg
    13561 gtcgctactc aggcagcgac tctcctcatg ggcaaaaaca ccgccgcttt cgtccgcaac
    13621 gccgcgccgg aagtggaagt ccacatcatc aacacttcga aggcctcgat cgacgagaag
    13681 aaggcagtcc agaagaagta cgtgaggttc tcgggctacc cgggcggcct caaggaaact
    13741 ccgcttcgca ggatggcgga ggagaagggc tattcggaag ttttccgcaa ggcggtcaag
    13801 ggcatgctcc cgaagaacaa gctccaggca gtgatgctca agaacttaac tatttccgaa
    13861 taattttatg gcagaagcaa ccaaaacgtc cgctcgaagc gcagcggcgg acaagaaaga
    13921 aaaatatatc gaagccgtcg gccgcaggaa gaccgcggtt gcgcgcgtgc gcctcgtcgc
    13981 ttcgagcaag atggcctata ccgtaaacgg caaggagctt gaaatctatt tcccgacgga
    14041 cgagctccag cgcatcgtac gcgacccgtt cacggcggca aaggacgcct caaaattcaa
    14101 gattaccgcc aagatcgtcg gcggcggcat ccactcgcag gcggaagcgc ttcgccacgg
    14161 gatcgcccgc gcgctcatca agagcgacat ggagctccgc aagaagatca agaaggcagg
    14221 cttcctcgag cgcgatcagc gcgcaaagga gcgccgcaag ttcggcctca agaaagcccg
    14281 caaggctcct cagtggtcta aacgatagaa aaaaaagagg gattaccctc tttttcttta
    14341 cgcttcaacg gccgcaggta tgtcgcagta gcaacgagcg ataccgagac gggtgagaaa
    14401 gcgagtgtac gcagctttcg caaggccgga ctgagcgaag gcgaaggagg cgggatcgct
    14461 acctcgcgta gtaacgcgag agttgtgacc gcgattttcc cgagcgacgg cgagcaaaat
    14521 actcgtgacc aaacgataat ctttggagcg ccgctcaaga gcagagcgat ctccatggtt
    14581 gaagcattca tttacaatcc atcctgctca tactataatt tttcctgcaa tgaacgacga
    14641 aaacaacact cacgacgaaa attttgacga ggaggcctca gaggagacag catccgaaga
    14701 agcggatgac gacgtcgctt tcgagccgga agacaacgag accccgctga gctcattcgg
    14761 caagtcgaaa ggcgatgccc tcgcgtcgct caaggagaag cttaaggcga gcgaggcgaa
    14821 agccgcggaa tatctcaccg atctc
//
LOCUS       DASZYS010000037        11814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_102890, whole genome shotgun sequence.
ACCESSION   DASZYS010000037 DASZYS010000000
VERSION     DASZYS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102890"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..489)
                     /locus_tag="VHF05_02270"
     CDS             complement(157..489)
                     /locus_tag="VHF05_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HVS79780.1"
                     /translation="MPFIPFDVGNGQDRENGYVPSLDLNDFIVRHPEATFFATALGSA
                     MEALGIADGDLLVIDRIAEPSLGKIVVTTQEGEFLLRRFDENTARLAEPESPFAIWGT
                     VIGIIRRF"
     gene            complement(493..687)
                     /locus_tag="VHF05_02275"
     CDS             complement(493..687)
                     /locus_tag="VHF05_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79781.1"
                     /translation="MKNVKMIIDLKNEISRVNRKIDFYIMMNMPYRKEAEYHKRLTLQ
                     LRKVARKNWLGRSMKLFALV"
     gene            complement(781..1356)
                     /gene="lexA"
                     /locus_tag="VHF05_02280"
     CDS             complement(781..1356)
                     /gene="lexA"
                     /locus_tag="VHF05_02280"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVS79782.1"
                     /translation="MEIETAKAKIRKFYEDRKTMPSYSEMAKLLGYKSKNSVYRLVEK
                     LLDDGFVGKDARGSLVPGKGFGTVRMLGFVEAGFPTAAEEELLDTVDVDRLLIGNRER
                     MFMLTVSGESMIDEGINDGDFILVERTAEAKDGDIVVAFIDGGYTVKFLRKKNGRAYL
                     QPANKNFPPIVPREDDELRICAVVRAVIRKY"
     gene            1700..1867
                     /locus_tag="VHF05_02285"
     CDS             1700..1867
                     /locus_tag="VHF05_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79783.1"
                     /translation="MAKDKDNRERGKNASRNNKDTNAADEEKKAQHNSDEMDPDETIR
                     GAMDDARDEDW"
     gene            complement(1913..3115)
                     /locus_tag="VHF05_02290"
     CDS             complement(1913..3115)
                     /locus_tag="VHF05_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVS79784.1"
                     /translation="MRRLPASRLLIEELPVLVAIGAWWYAASFARFSLPVLFMGPAIL
                     VGCIAFVSAKYGLRQGLLGSFGAALFLVSDNTAFSASGTDALAERAVMVCLSFASSLI
                     FSTMRQAVPAAATDIGKEGGALQTAISSEESAAADAAETLVIEVSESDLQVEEIASPR
                     FGRTEDFLHIARSSLAPRLGRIERAIMALKLHGKKLGDMETQGYIRRIESQFRRLDYV
                     MQRLLQFARIDFDDMPVKIETFDLAKVLAEAADLVQRESPEHRIIFLPKTGAFADGDR
                     FRVRKVVDHLIRNALRYSPPDGKIIVTTKEDSGLLVTSVRDFGSGLEEGRLSKMEAAF
                     ASDIPGAREGLGVGLYTANEIVRKLGGRMSVKSRKGKGSEFSFSLPKASSESSAAGES
                     PTYKLPEG"
     gene            complement(3226..5043)
                     /locus_tag="VHF05_02295"
     CDS             complement(3226..5043)
                     /locus_tag="VHF05_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVS79785.1"
                     /translation="MKTLLKYILKYKRVLILALVLAAVNQIFSLLDPQLLRILVDDYA
                     SHATELPVAKFLQGVSLVLVGMVATAFISRTAKAFQDYFVNVSVQRVGTEMYSEGVSH
                     SFSLPYAVFEDQRSGEILSKLQKARTDSQLFITNVVNVLFFSVVGVLFVIGYALYVHW
                     LVGLVYILMIPTLGAVTYTLGRKIKEAQSRITRESNALAGSTTETLRNVELVKSLGLE
                     EQEIGRLNDTNEKILKLELDKVKYVRRLDFVQGTLINALRTSLLLLMLWLMALGKLTL
                     GEFFTLMFYSFFIFSPLAQFSLVATSYYEAKTSTSQLEEVLALKSAEERSGGKKIGRI
                     ESVRYDDLSFSYGAGLGDTLKRVNIEVLPGKTVAFVGPSGSGKSTLVKLLAGLYQPTG
                     GKLLVNGIDSREIDFDDLRRRCGMVAQETQLFAGTIRENLLFVRPDATDDDCIDALRR
                     AAALGIIERKALDGAVHGLDTRIGEGGLKLSGGERQRLAIARALLRKPDILIFDEATS
                     SLDSITEKAITATMKEIAAERPDLLTIMIAHRLSTIIHSDVIYVLEKGMLVEHGTHAE
                     LVARDGLYNALWRQQTAADVEQSAPSGEETLIFSSPIRH"
     gene            5147..5389
                     /locus_tag="VHF05_02300"
     CDS             5147..5389
                     /locus_tag="VHF05_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HVS79786.1"
                     /translation="MARGSKSKYSAKQKRMAEHIEKGYEKRGTPKRTAEARAWATVNK
                     TYGGGTKGGSGHRHLNKASMKRGGSRSHAKRQRNSS"
     gene            complement(5469..5948)
                     /gene="cas2"
                     /locus_tag="VHF05_02305"
     CDS             complement(5469..5948)
                     /gene="cas2"
                     /locus_tag="VHF05_02305"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HVS79787.1"
                     /translation="MNILEDLWNAELNYKGTKVNGFGIPIFLINGCNQKTLSSTISRL
                     KSRDYILKTQQGWVLTEMGRKYYKSRRELRSFESPFKKNSPKNLIVMFDVPEERKYER
                     EWLRNHLRRFGYQMIQKSVWVGPSPLPREFTGYAKEIGIKKCIATFKLAKPYKKSSR"
     gene            complement(6095..7099)
                     /locus_tag="VHF05_02310"
     CDS             complement(6095..7099)
                     /locus_tag="VHF05_02310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HVS79788.1"
                     /translation="MPKNVLVTRKIPDVGIGMLKNAGFNVDILPGDQPHTQAELIDAL
                     QKKQYDAVLSLLTDQVDAKLFDAAPSVKIYANYAIGFNNMDVAEAMKRGITLTNTPGN
                     SFSFCIAEHAIALMLALSTRLVEGDRFVRAGKYKGWDPDIFVGTDLQKKKLGLIGAGH
                     IGERVAHHASRGFDMEVLYYDVVKNEKIEQEYGAKYFGTPEEVLKEADFVSLHVPLLD
                     STKHLINADRLKLMKPSAFLVNTSRGPVVDENALVDALKNKVIRGAGLDVFEFEPQLA
                     AGLAELENVVLTPHIASARESARNDMATLAAQNIVDFLEGREPKNKVNPPAKEPAAVA
                     "
     gene            complement(7243..7884)
                     /locus_tag="VHF05_02315"
     CDS             complement(7243..7884)
                     /locus_tag="VHF05_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVS79789.1"
                     /translation="MKIKKIGHCCLVLETGGKKIMTDPGSYTPAAESETGIDIVLITH
                     EHGDHFHVESLKKILAANPQVVVVTNSAVGKLLRAENIPFTQVEDSESESVNGILIEG
                     FGTTHALVYDSIPSVMNTGYFVDNSFYFPGDAFYNPGKEVQVLALPMAGPWLKLSEAL
                     DYLKLVRPTVAFNVHDGMIIPASSGFLAHLGQMAGQEDGTKFVYMGNGDIAEF"
     gene            complement(7968..8318)
                     /locus_tag="VHF05_02320"
     CDS             complement(7968..8318)
                     /locus_tag="VHF05_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79790.1"
                     /translation="MTVLIFDDDEDFLGRLNSRFKAQPLRGLRVNSVWCNNAAKAQKA
                     VEKYNPDILFADHMLGLRENAFEVAKKAAENGIHVFTTSGASPKVLEMYANLGIPHVP
                     KSQVADKILELFEK"
     gene            complement(8378..8773)
                     /locus_tag="VHF05_02325"
     CDS             complement(8378..8773)
                     /locus_tag="VHF05_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79791.1"
                     /translation="MAYKITANEEIIRGWIERRNGTPAIVRGSADTLQGLTILFGAEG
                     GRSDLEPISWEEFFDTFNSGRLLFKYSDAKIENGNPPGFTFISRDNVAAFDDMDEPIL
                     PEENDMARENIFGSAPPAFEMGLPPEEAE"
     gene            complement(8844..9524)
                     /locus_tag="VHF05_02330"
     CDS             complement(8844..9524)
                     /locus_tag="VHF05_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVS79792.1"
                     /translation="MTYKDREEAGRALAAEIRVPKGGMAVFALPRGGVAVAYQVASAL
                     EVPLDTIPVRKLSAPANRELAFGAVAPEGIEIKDEEKMLDLGITAAEAEEMAARERVE
                     LDKEMAHYRSGRFAEDANFDTALIVDDGAATGMSAEAALKAARVLYASARLVFAVPVC
                     STEARERIEKVADEVICGMVPPYFLAVGEWYDKFAEFSDDEVLYLLHTLHDERVFQTR
                     ISEAGSAS"
     gene            complement(9616..10209)
                     /locus_tag="VHF05_02335"
     CDS             complement(9616..10209)
                     /locus_tag="VHF05_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79793.1"
                     /translation="MNKQLIIAGGILLLLVLGAGAVFFAAPRLAPEGNPPAEEPRTEE
                     LNTYSSTELGLAFRYPANYELRQSSGEKGAEYEARIMLAEAGKADAAGGPVPGEPGMS
                     ITVDVYPLAGGMTAEKWIIDSPDSNYRFAAAPYRLVSIGGKEFATFSWSGLYEGDTAV
                     HAENGRLYVVTATYIAPDDMTRSDLWNVILPTFMTAP"
     gene            10345..10674
                     /locus_tag="VHF05_02340"
     CDS             10345..10674
                     /locus_tag="VHF05_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79794.1"
                     /translation="MTRRLKKIIVPFVILPLVTGGLLTFVVIVDQSDDADAVLTNTLP
                     AFPDIIARNAPSSLISISVAAIIVVSAALVFAFFFLFFLIENALVLSDPARSFVRNRS
                     PPRLKAA"
     gene            complement(10887..11429)
                     /locus_tag="VHF05_02345"
     CDS             complement(10887..11429)
                     /locus_tag="VHF05_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVS79795.1"
                     /translation="MKNTVIWIIVIVVLVLLGIYAVEHRSNPLDTGEEATTTPLQTDE
                     NGAEGQTGSETASSSVENAPIAATSGEQSGASADVNARANVAAPAAEKTFTVSGKNFT
                     FTPSTITVNKGDKVKIVFQNTEGTHSFQIDGYNVGTQTIAAGQEATVEFTADKAGSFQ
                     YYCSVGTHRQMGMVGTLVVQ"
     gene            complement(11495..>11814)
                     /locus_tag="VHF05_02350"
     CDS             complement(11495..>11814)
                     /locus_tag="VHF05_02350"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HVS79796.1"
                     /translation="GGAGGRGAAFGGITMGTILSKDDTSITISIPGGGSKIVFTSAST
                     TVDTTATGTMDDLTVGANVMVQGTPNSDGSMTAKSIELRPAGQERAFGPVTTGSQTAP
                     AQQ"
BASE COUNT         2585 a         3222 c         3171 g         2836 t
ORIGIN      
        1 ctctaataag ttggtctgca attatccaag cacttctttt gttaggtaaa tcgtctgtta
       61 atcgaataac ctttaggctg aactcaaagc atctttgttt taaatcagtt ttgggtttgg
      121 aattaaaatt ttgcattttg atttttgatt tctgaattaa aatcttcgga taattcctat
      181 gaccgttccc catatcgcaa acggagactc tggttcggca agacgggcgg tattttcatc
      241 gaatcgtcgg agcagaaact ctccttcctg cgtagtaacg acgatctttc ccaggctcgg
      301 ctccgcaatg cgatcgatga cgagcaggtc gccgtctgcg atcccaagcg cttccatcgc
      361 gctcccgaga gctgtggcga aaaaggtggc ttcgggatgg cggacgataa aatcgttgag
      421 atcgaggctc ggtacatatc cgttttccct atcctgcccg ttccccacgt cgaacgggat
      481 aaacggcata tcctatacca aggcaaacag cttcatcgat ctgccgagcc agttttttcg
      541 ggccactttg cggagctgaa gcgtgagccg cttatgatat tcggcttctt tccgatatgg
      601 catgttcatc atgatataaa aatctatctt tcggttgacg cggctgattt catttttgag
      661 atcgataatc atttttacgt ttttcatgac gatattttta agctgtttta taaaattcga
      721 cgtttgattt gaaactttct tgcaacttgc aaacttatga attcagatgc aatggttcga
      781 ctagtacttt cggatgaccg cgcgcaccac tgcgcatatt cggagctcgt catcttcgcg
      841 cgggacgatc ggcggaaaat tcttgttggc gggctggaga tacgctcggc cgtttttctt
      901 gcggaggaat ttcaccgtat atcctccgtc gataaacgcc accacgatgt ccccgtcctt
      961 cgcttcggcg gtgcgctcga cgagaataaa gtcgccgtcg ttgatcccct cgtcgatcat
     1021 gctctcgccg gagaccgtca gcatgaacat ccgctcgcgg ttgccgataa gcagcctgtc
     1081 gacatcgacc gtgtcgagaa gctcttcttc ggcggccgtc gggaatcccg cttcgacgaa
     1141 gcccagcatc cttaccgtcc cgaacccctt gcctggaaca agcgagccgc gggcatcctt
     1201 gccgacgaat ccgtcatcca agagcttctc gacgagtcga tagacggaat ttttcgactt
     1261 gtaaccgagg agttttgcca tctcgctgta ggacggcatg gtcttcctgt cttcgtaaaa
     1321 cttccgaatt tttgcctttg cagtttcgat ttccatcccg ttagagatag gaaatgctat
     1381 gcatttccgt ggttgataat atttaagaaa acgtaccgcc tatttgcttg aattctcgtt
     1441 agagtctgag aaattaactg aaggatctct aacgggatcc atggttatat ggtatgtgaa
     1501 cgttcgttca catgcaaggt ggataacgca tcgtgaaatg gaatcgggcc ttccgtcaaa
     1561 atagtttctg aaaaacgcct ttgcggagcc tcgctggcga cgggcacttt aaaaagacag
     1621 gaactttttt tcactgccgg ttcatggccg gagagtagag tggaggaggc cggaattatc
     1681 cgatattaaa catattgcta tggcaaaaga caaagacaat cgcgaacggg ggaagaacgc
     1741 ttcccgaaat aataaggata ccaacgcggc agacgaggag aagaaggcgc agcacaactc
     1801 cgacgaaatg gatcctgacg agaccattcg cggagccatg gatgacgctc gcgacgaaga
     1861 ctggtaataa gaaggagcga aaatgcccgc acgatgtgcg ggcattttca tttcaacctt
     1921 cgggaagctt gtacgtgggg gattcacccg cggctgatga ttccgagctt gctttcggaa
     1981 gcgagaaaga aaattccgaa cccttccctt tcctgctttt cacgctcatc cgtccgccga
     2041 gcttccggac gatctcattg gcggtataga ggccgacgcc gagcccttcc cgcgcgccgg
     2101 ggatgtccga cgcgaacgcc gcttccatct tggaaaggcg cccttcctcc agtccggatc
     2161 caaaatcgcg cacggatgtt acgagcaagc ccgaatcctc tttcgtcgtt acaataatct
     2221 tgccgtcggg aggcgaatac ctgagagcgt tgcggatgag atgatcgacg acttttctca
     2281 cgcggaacct gtctccgtcg gcgaacgcac cagttttcgg caggaaaatt atcctgtgct
     2341 ccggcgattc gcgttggacg agatcagccg cctcggccaa gactttcgcg aggtcgaatg
     2401 tctctatctt caccggcatg tcgtcgaaat cgatgcgcgc gaattgaagg agcctctgca
     2461 tcacgtagtc gagccgcctg aactggctct cgatccgccg aatgtagcct tgggtttcca
     2521 tgtcgccgag ctttttgccg tggagcttga gcgccatgat cgcgcgttcg attctcccga
     2581 gccgcggcgc aagcgaactg cgcgcgatat gaaggaaatc ttcagttcgc ccgaaacgcg
     2641 gcgacgcgat ctcttcaact tgaagatcgg attccgacac ctcgataacg agcgtctcgg
     2701 ctgcatcggc tgccgcggat tcctcggagc ttatcgcggt ttggagcgcg ccgccttcct
     2761 tgccgatatc ggttgccgcc gccggcacag cctgccgcat ggtggaaaat atgagcgacg
     2821 atgcgaacga aaggcagacc atcaccgcgc gttcggcaag cgcgtccgtt ccggaagccg
     2881 aaaaagccgt gttgtcggat acgagaaaca aggcggcccc gaacgagccg agaaggccct
     2941 gccgcaaccc gtacttggca gaaacgaacg cgatgcatcc gacgaggatt gccggcccca
     3001 tgaaaagcac gggcaaagaa aaccgcgcga aagaagcggc gtaccaccat gctccgatcg
     3061 cgacaagaac gggcagttcc tcgatcagga ggcggcttgc cggaagccga cgcaccgcac
     3121 cggagtccgt tcgggctggg agtcgatgtg aatggcctat atcgttcatt ggaaaaactc
     3181 tccaccgcgg acgggcagag agcggtaatt tgagtatagt ttcgatcaat gtcggattgg
     3241 tgaagaaaaa atgagtgttt cttctcccga aggagcgctt tgttccacgt cggcagccgt
     3301 ctgctggcgc cagagcgcgt tgtagagtcc gtcgcgggcg acaagttcgg catgggtgcc
     3361 atgctcgacg agcattcctt tctcaaggac ataaatgaca tccgagtgga tgatggtcga
     3421 gagccggtgc gcgatcatga tcgtgagaag gtcggggcgt tcggcggcga tttccttcat
     3481 cgtggcggta atcgcctttt cggtgatcga atcgaggctc gacgttgctt cgtcgaagat
     3541 gaggatgtcg ggcttccgca ggagcgcgcg ggcgatggcg aggcgctggc gttcgccgcc
     3601 cgaaagcttc aagccgccct cgccgatcct cgtgtcgagt ccgtgtacgg cgccatcaag
     3661 tgccttgcgc tcgatgatgc cgagagccgc ggcgcggcga agcgcgtcga tgcagtcgtc
     3721 gtcggtcgcg tccggccgca cgaacaggag gttctcgcgg atcgtacccg caaaaagctg
     3781 ggtttcctgc gccaccatgc cgcatctccg gcggagatcg tcgaagtcga tctcgcggct
     3841 gtcgatgccg tttaccagga gcttgcctcc ggtcggctgg tagaggcctg caagaagctt
     3901 cacgagggtc gatttgcccg agccggaggg acccacgaac gcgaccgtct tgccaggaag
     3961 aacctcgata ttcacccgct tcaaggtgtc gcctaagcct gcaccatagc tgaacgagag
     4021 gtcgtcgtag cggaccgatt cgatcctgcc gattttcttt ccgccgctcc tctcctccgc
     4081 ggacttgagt gcgagcactt cctcaagctg ggaggtggag gttttcgcct cgtagtagga
     4141 cgtcgcgacg agtgagaact gggcaagcgg gctgaagatg aagaacgagt agaacatgag
     4201 cgtgaagaat tcgcccaaag tgagcttccc gagcgccatg agccagagca tgagaaggag
     4261 aagcgacgtg cgcagggcgt tgatgagcgt gccctggacg aagtcaaggc ggcggacgta
     4321 cttcaccttg tcgagctcga gcttcaggat tttctcattg gtgtcgttca gccttccgat
     4381 ctcctgctcc tcgaggccga ggcttttcac gagctcgaca ttgcggaggg tttcggtcgt
     4441 cgatcccgca agcgcgttcg attcgcgggt gatgcggctc tgggcttcct tgatcttgcg
     4501 gccgagggtg taggttaccg cgcccagggt cggaatcatc aggatataga ccaggccgac
     4561 cagccagtga acgtagaggg cgtagccgat cacgaacaaa acgccgacga ccgagaagaa
     4621 caggacgttc acgacattcg tgatgaaaag ctgggaatcg gtgcgcgctt tctggagctt
     4681 cgacagaatt tcgccggacc gctggtcctc gaataccgcg tacgggagcg aaaacgagtg
     4741 gctcacgccc tccgaataca tttcggtgcc gacacgctgg accgagacgt taacgaaata
     4801 atcctggaaa gctttggcgg tgcgcgaaat gaatgcggtc gcgaccatgc cgaccaggac
     4861 gagcgacacg ccctggagga acttcgcgac aggaagctcc gtggcatggg atgcgtagtc
     4921 gtcgaccagg atgcggagaa gctgggggtc gagaagcgaa aatatctggt ttacggctgc
     4981 gagaacgagt gccaagatga ggacccgctt gtatttcagt atgtatttta aaagagtttt
     5041 cattgatatt tttggtttcc tgcagagttc ggcgtttccg ggaatttcac acctttttca
     5101 ctcctcgcgg ctagtctata catattagca gatttatttt aaacatatgg cccgaggaag
     5161 caaatcgaaa tattcggcga agcagaagcg aatggcggag catatcgaga aaggatatga
     5221 aaagcgcggc acgcccaagc gcactgccga ggctcgagcc tgggcgacgg tgaacaaaac
     5281 ctatggcggc ggaactaaag gaggttcagg tcatcggcat ctcaataagg cttcgatgaa
     5341 acgtggtgga agccgctcgc atgcaaaaag acagcgcaac tcttcttagc attggtaagt
     5401 tgaagtgcgt aaaacttaaa tccttgctaa attgacactt tccgcgatcg cggaaagtgt
     5461 caatttaatt aacgggaact tttcttgtaa ggcttggcga gtttgaatgt tgcgatgcac
     5521 tttttaatcc cgatctcctt cgcataacca gtgaattccc ttggaagcgg gctcggaccg
     5581 acccagacgc tcttctgtat catctgatag ccgaatctgc gcaaatgatt cctcagccat
     5641 tccctctcat attttctttc ttcaggaaca tcgaacataa caatcaggtt cttcggagag
     5701 ttcttcttga atggtgattc gaaactgcgt aattcacgtc gactcttata atatttcctt
     5761 cccatttcag ttaaaaccca tccctgttgc gtcttaagaa tgtaatctct gctctttaag
     5821 cgagatatcg tactcgacag ggttttttgg ttgcatccat tgataagaaa tatcggaatg
     5881 ccgaatccat taaccttcgt tcccttgtaa ttgagctcgg cgttccacag atcttccaaa
     5941 atgttcatga caagactcat ataattatga tatgttgcgg aagttaaatt gccaacttcc
     6001 acgatcgcgg aagttggcaa tttctccaac agcatttgtt gaggttaaac ctcaacaaac
     6061 gcgaaacgag ggcccgtcct tcgtttttgt gtttttatgc tacggcggct ggttccttcg
     6121 cgggaggatt taccttgttc ttcggttccc tgccttcgag aaaatccacg atgttttgcg
     6181 ccgcgagcgt agccatgtcg ttgcgggcgc tctcgcgggc cgaagcgatg tgaggagtga
     6241 ggacgacatt ctcaagctcg gcaagccccg cggccaactg gggttcgaat tcgaagacgt
     6301 cgagacctgc gccgcggatc accttgttct taagcgcatc cacgagcgca ttctcgtcga
     6361 cgaccggccc gcgggaggta ttgacgagaa aggcggaggg cttcatgagc ttgagccgat
     6421 cggcgttgat gaggtgcttc gtcgagtcga gaaggggcac gtggagcgat acgaagtcgg
     6481 cttccttgag cacctcttcg ggcgtgccga agtacttcgc gccgtattcc tgctcgatct
     6541 tctcattttt cacgacatcg tagtaaagca cctccatatc gaagccgcgc gaggcatgat
     6601 gggcgacgcg ctcgccgata tgccccgcgc cgatgaggcc gagctttttc ttctgaaggt
     6661 cggtgccgac gaagatgtca ggatcccaac ccttgtattt ccctgctcgt acgaaccggt
     6721 cgccttcgac cagccgcgtc gagagcgcga gcatgagcgc gatcgcatgc tccgcgatgc
     6781 agaagctgaa ggaattgccc ggcgtattgg tgagcgtgat gccgcgcttc atcgcctctg
     6841 ccacatccat attattgaag ccgatagcgt agttggcata tatttttact gaaggcgccg
     6901 catcaaaaag cttcgcatca acctggtcgg tgagaagcga gagaacggca tcgtactgct
     6961 tcttttggag cgcatcgata agttcggcct gggtatgagg ctgatcgccg ggaagaatgt
     7021 cgacattaaa tccggcgttt ttgagcatcc cgatgccgac atcgggaatc tttctcgtca
     7081 caagtacgtt ttttggcatg gctataatct tgagtctagt tttacttcgc tatcatatca
     7141 cagcgccact agccctctta tgcaccattg acaaatcatc aatttaaagc ggcattagta
     7201 caataaataa aatacgaatg ccgtttttaa aatttgcccg gattagaact ccgcaatatc
     7261 gccattaccc atatagacga acttcgttcc gtcctcctgc cctgccattt gcccgagatg
     7321 cgccaggaag ccgctgcttg cgggaatgat catgccatcg tgcacgttga atgcgacagt
     7381 ggggcgtacg agcttcaaat aatcgagcgc ttcggaaagc ttgagccacg gccccgccat
     7441 gggaagcgcg agtacctgga cctccttgcc cggattgtag aacgcgtcgc cagggaaata
     7501 aaacgagttg tcgacgaagt agccggtatt cattacggac ggaatcgaat cgtacacgag
     7561 cgcgtgcgtg gtgccgaatc cttcgatcaa gattccgttc accgactcgc tctcgctgtc
     7621 ctcgacttgc gtgaatggaa tgttttcagc acggagaagt tttccgaccg ccgagttggt
     7681 tactacaacc acctgagggt tggcggccag aatcttcttg agcgattcga cgtgaaaatg
     7741 gtcgccgtgc tcatgggtga tgagcacgat atcgatgccg gtctcgctct cggccgccgg
     7801 cgtatatgag cccggatcgg tcattatttt ctttccaccg gtttcaagca caaggcagca
     7861 atgcccgatt tttttgattt tcatagtggt tagtataatc caaaattcct gaatttagac
     7921 tgaacaaaag gcgcgccttg gttaaaggcg cgccttgcag taattgctta tttttcgaaa
     7981 agctcgagaa ttttatccgc aacttgcgat ttcggcacat gcgggatgcc gaggttcgca
     8041 tacatctcga ggactttcgg cgaagccccc gacgtagtaa aaacatggat tccattctct
     8101 gccgcttttt tcgcaacttc aaaagcgttt tcacgaagcc cgagcatgtg atcagcaaag
     8161 aggatatcag ggttatactt ctccactgct ttctgcgctt tcgcggcatt gttgcaccac
     8221 acggaattga ccctaagtcc cctcaaaggc tgtgctttga accgtgagtt caaacggccc
     8281 aaaaagtcct cgtcatcatc aaaaatcagt acggtcatac agctccttcc gagaaattta
     8341 taccataaaa attatatttt gcaacacctc tctgttccta ctccgcctct tccggcggca
     8401 gccccatctc gaaggcgggc ggagcgctgc cgaagatatt ttcgcgcgcc atgtcgtttt
     8461 cctcgggaag aatcggctcg tccatatcgt cgaaagcggc gacattgtcg cgcgatatga
     8521 atgtaaaacc gggaggattg ccgttctcaa tcttggcgtc gctgtatttg aaaagaagcc
     8581 ttccggagtt gaacgtgtcg aagaattcct cccatgaaat aggctcgaga tcgcttcgtc
     8641 cgccctccgc gccgaagagg atcgtgagcc cttgaagcgt atccgcgctt ccccgcacga
     8701 ttgcaggcgt gccgttgcgc cgttcgatcc agcctctgat tatctcttcg tttgccgtga
     8761 ttttgtatgc catgggcaca gagtagcatc gatgcctaaa gacatcgtga acctgaatgc
     8821 ggccgcgacg gcatcattcc cgattacgac gcgctgccag cctcagagat tctcgtctga
     8881 aaaacccttt catcatggag ggtgtggaga agataaagca cctcgtcatc cgagaactct
     8941 gcgaacttgt cataccattc cccgaccgcc agaaaatacg gcggcaccat tccgcagata
     9001 acctcgtcag ctactttttc tatgcgctca cgcgcttctg tcgagcacac aggaacggca
     9061 aagacgaggc gggcggacgc atagaggacg cgcgcggcct tgagggcggc ttccgcgctc
     9121 attcccgtgg ccgcgccatc gtcaacgata agagcggtgt cgaaattcgc atcctcggca
     9181 aatcgtccgc tccggtaatg cgccatttcc ttatcaagtt cgacgcgctc gcgggccgcc
     9241 atttcttccg cttcggcggc ggtaatgccg agatcgagca ttttctcctc atccttgatc
     9301 tcgatcccct ccggcgctac tgctccgaat gccaattccc ggttcgccgg tgcggagagt
     9361 tttcgcacgg gaatagtgtc gagcgggacc tccagggcgg acgccacctg gtacgcgacc
     9421 gcgacgcctc cccgcggcag ggcgaacacc gccatgccgc ctttcggcac gcggatttcc
     9481 gcggcaagcg cgcgaccagc ctcctcccga tctttatacg tcatacgacc attttcgcgc
     9541 ctgggcatga aaatggaatt aatttttatt cagctcatca gcatttcatt ccgcagcttg
     9601 aaacgaggcg cttcgctaag gagccgtcat aaacgtcgga aggatcacat tccataaatc
     9661 gctcctggtc atgtcgtcgg gcgcaatgta tgtcgcggta acgacgtaga ggcggccgtt
     9721 ttccgcatgc acggccgtat cgccttcgta tagtcccgac cagctgaaag tcgcgaattc
     9781 cttgccgccg attgacacca gacggtatgg cgcggcggca aagcgataat tcgaatcggg
     9841 agagtcgata atccattttt cggctgtcat gcctcccgca agcggataga cgtcgacagt
     9901 gatgctcatg cccggctcgc caggaacggg gccgcccgcc gcatccgctt ttccggcttc
     9961 ggcaagcatg atgcgcgcct catattccgc tcctttttct ccactggact ggcgaagctc
    10021 gtaattagca ggatagcgga atgccagtcc gagttcagtc gaggaatacg tattcagttc
    10081 ttccgtccgg ggctcttccg cgggaggatt tccttcggga gcaagacgag gcgccgcgaa
    10141 aaagaccgcg cccgccccga gcactaaaag gagaagtatt ccgcctgcga taatgagctg
    10201 tttgttcata ttggaacctc aattatatac cgggaagaac acgatctgtt cttgaagcgt
    10261 ggaagtatgt atcataaccg cacatttttt ataaaaatca acgtaataac gccgcgccgg
    10321 aggagtataa taggtatata aagaatgacg cgcagactga aaaaaatcat agttcccttc
    10381 gtcattctgc ctctcgtgac aggcggcctc ctcacgttcg ttgtcattgt cgaccaatcc
    10441 gacgatgctg atgcggtact cacgaacact ctgccggctt ttcctgacat catcgccagg
    10501 aacgccccga gttccctcat ctcgatctcc gtcgcggcga tcatagtcgt ctcggcggcc
    10561 ctcgtcttcg cgttcttttt ccttttcttt ctcattgaga atgccctggt tctctccgat
    10621 ccggcgcgtt ctttcgtccg caatcgctct cctccgcgcc tcaaggctgc ctgaaggcaa
    10681 accctccggc agtccgtcgc cgccggacgt taatggcgga caaactccta atgcacactc
    10741 ctttctctct gttctgggat aatggggaga tacacggaaa cacacaacct atgggcttct
    10801 cccataggtt gttccgttta tgaacccccg aattttacgt acttgtacgt tgactgctcc
    10861 gttatctccc gagtactgcc ccgcttctat tggacgacca gcgttccgac catgcccatc
    10921 tggcgatggg tgcccacaga gcagtagtat tggaagctgc cggctttgtc cgcggtaaac
    10981 tcaaccgtcg cttcctgtcc ggcagcgatc gtctgcgtgc cgacgttgta gccgtcgatc
    11041 tggaacgaat gggtgccctc ggtattttgg aatacgatct tcaccttgtc gcccttgttg
    11101 acggtgatgg tcgagggagt gaacgtgaag ttcttgccgc tcaccgtgaa tgtcttctcg
    11161 gctgccggcg cggcgacgtt cgcgcgtgcg ttaacgtcgg cgctcgcgcc gctttgctcg
    11221 ccggaagtgg cggctatcgg cgcattttcg acgctcgagg aagctgtctc gcttcccgtc
    11281 tggccttccg cgccgttttc gtcggtttgg aggggagttg tggtcgcctc ttcgccggta
    11341 tccaatggat tcgacctgtg ctctactgcg tagatgccga gaagcacgag aacgacaatg
    11401 acgataatcc aaattactgt atttttcata agcgctgttt atatgtaatt gtacaattat
    11461 acacccgggg ccttcgcctc gggtgtggaa accgctattg ctgcgccggc gccgtttgac
    11521 tgccggtcgt aaccggcccg aacgcccttt cctggcccgc aggccgaagt tcgatcgatt
    11581 tcgccgtcat gctgccatcg gaattggggg ttccctgcac catcacgttc gcaccgacag
    11641 taagatcgtc catcgtccca gtggcggtcg tgtcgacggt cgtcgaagca gaggtgaaca
    11701 ctattttcga accgcctccg ggaatgctta tggtgatgct cgtatcatcc ttggaaagga
    11761 tcgtgcccat agttatgccg ccaaacgccg cgccgcggcc tcctgctccg cctt
//
LOCUS       DASZYS010000038         7772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_417108, whole genome shotgun sequence.
ACCESSION   DASZYS010000038 DASZYS010000000
VERSION     DASZYS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417108"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..198)
                     /locus_tag="VHF05_02355"
     tRNA            complement(127..198)
                     /locus_tag="VHF05_02355"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Cys,seq:gca)
     gene            complement(240..575)
                     /locus_tag="VHF05_02360"
     CDS             complement(240..575)
                     /locus_tag="VHF05_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HVS79797.1"
                     /translation="MYHDERLAEQIRELAAEFISREASKQSLITVTSVRLYDDNRNAT
                     VFFTAYPESHEKTALEFLKRKRPDFKEYVKRNAPIGRIPFFDFEIDLGEKNRQRIEEL
                     SQEDKKDEG"
     gene            complement(644..2131)
                     /gene="infB"
                     /locus_tag="VHF05_02365"
     CDS             complement(644..2131)
                     /gene="infB"
                     /locus_tag="VHF05_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HVS79798.1"
                     /translation="MASLRPPVIVIMGHIDHGKSTLLDYIRKSNIVEREAGGITQHVA
                     AYEVKHAAADGTERRITFLDTPGHAAFSSIRERGGRIADIAVLVVSAEDGVKPQTLEA
                     LEVIKDEKLPFIVAINKIDKPAADIIRTKTSLAENGIYVEGFGGSVSCVQISAKTGEG
                     VPDLLDMMLLTADLEDFRGDPSLPASGYVLESNLDPKKGLSATLIIKNGTLKGGQFVA
                     AGSATAPVRIMEDFQGKPLKEATFSSPVRIIGWDKLPAVGEEFKVFETKNDALEFAAA
                     FAEQSPTSNEPLPDPESGVTVIPVIVKGDTVGSLEAIQKELAKLSTERVAVRIVQSGI
                     GTLSENDLRNAEGIKNTVIVSFNAKIDATARAYALRQGLEIHEFNIIYKLTEWMEDIV
                     AERTPKVLTDVLAGEAKVLKIFSKTKDRQVVGCRVESGSLKNGATVKVMRRDAEIGEG
                     RIRELQHNKNKTSEVTEGMEFGALVEFSIDLAPADRLQAFETVEK"
     gene            2258..2476
                     /locus_tag="VHF05_02370"
     CDS             2258..2476
                     /locus_tag="VHF05_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79799.1"
                     /translation="MTVEEGKEMLRECMINEDWFEKYGIHAIGLPHGRVTIYSENPLA
                     QDIKEEIRSRVEGVDVYFEVEGQATASV"
     gene            complement(2552..3034)
                     /locus_tag="VHF05_02375"
     CDS             complement(2552..3034)
                     /locus_tag="VHF05_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HVS79800.1"
                     /translation="MNFSGTDYLPFVVLGLALVVAVLIAWIIRLELRLKRMLRGKNAK
                     SLEETITGVIRDLNFLKEYRKDSLGYLEAIEGRIKKSVQAIETLRFNPFKGVGEGGNQ
                     SFATALINENGDGVVISTLHARDRMSLFAKPVKNFSSEHDLTEEETHVIEQAKKSLSH
                     "
     gene            3229..4419
                     /locus_tag="VHF05_02380"
     CDS             3229..4419
                     /locus_tag="VHF05_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVS79801.1"
                     /translation="MENKPNNRIFMDHASTTPVDPRVLEAMTPFFSEKFANPSAIYRE
                     GVEAGETVRVARLEIAKLLHCRADEIVFTASGTEADNMAVFGAFRRAQTLRIAKPHIV
                     TTAIEHPAVLEAVRAVEADGASATYVPVSEDGLVDPKKMAEALSPETVLVSVMLANNE
                     IGTVQPVARIARAVREYKEKLGRGKTDFPYFHVDASQGPNYLECFADKLAPDLMTLDA
                     SKIYGPKGIGLLFVRKGIALEPLIYGGGQERGLRSGTENVPFIVGFAEALRIAEEERE
                     AEAARLTELRDRMIEEILKKFPHASPNGSRVERLANNVNICFPGLDAEFAVIQLDQKG
                     IQCSYSSSCRTLAENNNSYVIEALGKKDCAASSLRFTLGRSTTKEDVEKALGAIVDIV
                     APRM"
     gene            5097..>7772
                     /locus_tag="VHF05_02385"
     CDS             5097..>7772
                     /locus_tag="VHF05_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS79802.1"
                     /translation="MPPFGQFTTKAKEAIRKAHELAIERGQNHVNPLHLLTALILQEE
                     SMVTSILDKLEIDSILLTDSLLEAVESPDAAGTLSPSYQIYLTPELAHAIENSSKIAA
                     RLNDEFVSTEHLFIALMEVPGTAREILAKFRIDKEKVLRVLEELKSGKTTDVSQPKKF
                     KALAKYTKSLTKAAANNKLDPVIGRDEEISRIIQILSRRTKNNPILIGEAGVGKTAIV
                     EGLATRIATGNVPESLRDKELVSLDLGLLIAGTKYRGEFEERLKNILKEVEKSDGKVI
                     LFIDEIHTIVGAGSAEGSIDAANMLKPALARGELRAIGATTFKEYQKHIEKDPALTRR
                     FQPVFVNEPSIDDAITILRGLKEKYELYHGVRITDDAIRAAVNLSSRYITDRFLPDKA
                     VDLIDESASHLKISLENMPPALQETHSRIVKLEIEREALKKEVGEDGVAKSAETKSRI
                     KDIEKEIADLKEKTAELELKWKNEKETVGDIKSIKKDLEALRLEAEGAEARADLSLAA
                     EIRYGKIPQLQKEYELKSKRLKRLQSSRRILKEEITENDIADIVSRWTGIPVSRMLEE
                     EAEKLARMDEELDKRIIGQKEAVKKIADTVKRSRAGIADPNRPIGSFIFLGPTGVGKT
                     ELTKALAEFMFDDEKALIRLDMSEFMEKHSVSKIIGAPPGYVGHEEGGNFTETVRHRP
                     YSVILFDEIEKAHPEVFNILLQVLDNGRLTDAKGRTVNFRNTIIILTSNIGAQYIDKM
                     QKIGFALGDKDKQNYEEMKDKVMEALKNHFRPEFLNRLDDIVLFDALSKDAVKEIVKI
                     QIGLVKERLTQKEISLEVTPDALDLLAEKGYDPQFGARPLKRLIQTKILNPVASLIIS
                     KGIAKGGMVSVGTKNGEFTFDVKKGRRGALIAENLI"
BASE COUNT         1764 a         2129 c         2194 g         1685 t
ORIGIN      
        1 tccctttcta tgagttagga agtttggacg tttgtgacgg tgattgatct aagcaccaat
       61 gtgctaaacc aaaaaccatg ctagcggatt ttgaggttgt gtcaaccgcg aggaggaaga
      121 tgagtgaggc cacggcggga atcgaacccg cgcatataga ttttgcagat ctacgactta
      181 ccacttgtct acgtggccct tgtgtccgta ttgtagcttg gtatcttgtt tgctgtctat
      241 tatccttcgt ccttcttgtc ttcctggctc aactcctcga tgcgctggcg gtttttctcg
      301 ccgagatcaa tctcgaagtc gaagaagggg atgcggccga tcggcgcgtt ccgcttcacg
      361 tattccttga agtccgggcg cttgcgcttc aggaattcga gcgcggtttt ctcgtgcgat
      421 tcgggatatg ccgtgaagaa tacggtcgcg ttcctgttgt cgtcatagag gcgcacggat
      481 gttaccgtga taagcgactg cttgctcgct tcgcggctga tgaattccgc cgcgagctcg
      541 cgaatctgct ccgccaatct ttcgtcgtga tacataacta aaatttagaa gtcaaaattc
      601 aaaagtcaaa taaagttttg aattttacat tttgcatttt gatttacttt tctactgttt
      661 cgaatgcctg aaggcggtcg gcaggggcga ggtcgatgga gaactcgaca agggcgccga
      721 attccattcc ctcggtgacc tccgacgtct tattcttgtt gtgctggagc tcgcggatgc
      781 gcccctcgcc gatctccgcg tcgcgccgca tgaccttgac cgtcgcgcca tttttaaggc
      841 tgccgctttc gacccggcag ccgacgacct ggcgatcctt cgtcttgctg aaaatcttga
      901 ggactttcgc ttcgccggcg agcacgtcgg tgaggacttt cggcgtgcgc tccgcgacga
      961 tgtcctccat ccattcggtg agcttgtaga tgatattgaa ttcgtggatt tcgagtccct
     1021 ggcggagtgc gtaggctcgg gcggtcgcgt cgatcttcgc gttgaagctc actatgacgg
     1081 tgttcttgat gccttccgca ttgcgaaggt cgttctcgct caacgtcccg atgccgctct
     1141 gtacgatgcg tacggccacc ctttccgtcg agagcttggc gagctccttt tggatcgctt
     1201 cgaggcttcc cacggtgtcg cctttgacga taaccggtat gacagtgact cctgattcgg
     1261 gatcggggag aggttcgttc gatgtagggc tctgttcggc gaacgcggcg gcgaattcga
     1321 gcgcgtcatt ttttgtctcg aaaaccttaa attcttctcc gacagcaggc agtttgtccc
     1381 agccgatgat gcgcaccggc gaggagaacg tcgcttcctt gagcggcttg ccctggaagt
     1441 cttccatgat ccgcaccggc gccgtcgcgg agccggccgc aacgaactgg ccgcccttaa
     1501 gcgttccgtt cttgatgatg agtgtggcgg aaaggccctt tttcggatcg agattgctct
     1561 ccaatacata gcccgaagcg ggaagcgacg gatcgcccct gaaatcctcg aggtcggcgg
     1621 tgagaagcat catatcgaga agatccggca cgccttcgcc ggtcttcgcg gaaatctgca
     1681 cgcaggaaac gctgccgccg aatccctcga cataaatgcc gttctcggcg aggctcgtct
     1741 tggtgcggat gatatcggca gccggcttgt cgatcttgtt gatcgcgacg atgaagggaa
     1801 gcttctcgtc tttgatgacc tcgagcgcct caagcgtctg cggcttcacg ccgtcttccg
     1861 ccgacacgac gagcaccgcg atatcggcga tgcggccgcc gcgttcgcgg atggaagaga
     1921 atgccgcgtg gcccggagta tcgagaaagg tgatgcgccg ctcggtgccg tcggcggccg
     1981 cgtgcttcac ttcataggcg gcgacgtgct gggtgatgcc gcccgcttcg cgctcgacga
     2041 tgttcgactt gcggatatag tcgagaagcg tcgacttgcc gtggtcgatg tggcccataa
     2101 tgacgataac tggcggcctc aatgaagcca tattctgctg tggggaattt tgggccataa
     2161 cagtttctta ctctacaccg aaatgagagt atgtaaagat tagctttatc gtttgactct
     2221 gcggttgttc gggtatttac tacgagcagg aggaactatg actgtcgaag aaggcaaaga
     2281 gatgcttcga gaatgcatga taaatgagga ttggttcgaa aagtatggaa ttcatgcgat
     2341 cggcctgccg cacggacggg tgactattta tagcgaaaac ccccttgcac aagacatcaa
     2401 agaagaaatt cgcagccggg tcgagggcgt agacgtgtat tttgaagttg aggggcaggc
     2461 gacagcgtct gtgtgaagtt caaagagata gccgaaatcg cgactatctc ttttttacag
     2521 cgtaaggcgc tgagctgcgg cgcgtaagat gttaatggga aagtgatttt ttcgcctgct
     2581 cgatgacgtg tgtttcttct tcggtgaggt cgtgctcgga ggagaaattt ttcaccggct
     2641 tcgcgaagag gctcatgcgg tcgcgcgcgt ggagcgtcga gatgacgacg ccgtcgccgt
     2701 tttcattgat aagcgcggtc gcgaagctct ggttgccgcc ctcgccgacg cctttgaacg
     2761 gattgaagcg gagcgtctcg atcgcctgaa cgcttttctt gatgcggcct tcgatcgctt
     2821 cgaggtagcc caggctgtcc ttgcggtatt ccttgagaaa attgaggtcg cggatgacgc
     2881 cggtaatcgt ctcttcgagg ctcttggcgt ttttgccgcg gagcatgcgc ttcaagcgca
     2941 actcgaggcg gatgatccag gcaataagca cggccacgac gagtgcgagc ccgaggacga
     3001 caaaggggag ataatcggtg ccggaaaaat tcatagttac agattatacg ataaatgctg
     3061 aaatttacaa ttttcaattt ctaatttcca atgaattttc aacataatca caataaaaac
     3121 ggcagcttga aaaaattagg tcatagggcc ttgattggaa attgaaaatt gcgcattgaa
     3181 aattttagta gcattataga tgcaacatat cccgcatggc catctgatat ggaaaacaaa
     3241 ccgaataatc gaatcttcat ggatcatgcg tcgacgacgc ccgtcgaccc gcgggtgctt
     3301 gaggcgatga cgccgttctt ctcggaaaaa ttcgccaacc cttcggcgat ctatcgcgag
     3361 ggagtcgaag ccggtgagac ggtcagggtg gcgcggctcg agatcgcgaa gcttcttcat
     3421 tgccgcgctg atgagatcgt ttttaccgcg agcggcaccg aagccgacaa tatggcggtc
     3481 ttcggcgcgt tccgccgcgc tcagacgctc cgcatcgcaa agccgcatat cgtgacgacg
     3541 gcgattgagc acccggcagt gctcgaggcg gttcgcgcgg tcgaagccga cggggccagc
     3601 gcgacgtacg tgccggtttc ggaagacggc ctcgtcgatc cgaagaaaat ggccgaagcg
     3661 ctctctcccg agacggtgct ggtgtcggta atgctcgcga acaacgaaat cggaacggtt
     3721 cagcccgtgg cgcggatcgc gcgcgccgtc cgcgaataca aggagaagct cggccgcggc
     3781 aaaacggatt tcccgtattt ccatgttgat gcgagccagg gaccgaacta tctcgaatgt
     3841 ttcgccgaca agctcgcgcc cgacctcatg acactcgacg cttcgaagat atacggcccg
     3901 aagggaatcg gcctgctgtt cgtgcgcaag ggaatcgcgc tcgagcctct tatatatgga
     3961 ggagggcagg agagggggct ccgatcgggg acagagaacg tgccgttcat tgtcggcttt
     4021 gcggaagccc tcaggatcgc ggaggaggag cgcgaggcgg aagcggcgcg gctcactgag
     4081 cttcgcgatc gtatgatcga agaaattctg aaaaaatttc cgcacgcgtc gccgaacggc
     4141 tcgcgcgtcg agcggctcgc gaacaacgtg aatatttgct ttccgggact cgatgcggaa
     4201 ttcgcggtca tccagcttga tcagaagggg atacagtgct cgtactccag ctcgtgccgg
     4261 acgctcgcgg aaaacaacaa ctcctatgtg atcgaagcat tggggaaaaa agactgcgcc
     4321 gcatcgtcgc ttcgcttcac gctcggccgc tcgacaacga aggaggacgt agaaaaagcg
     4381 cttggagcga ttgttgacat agttgcgcct cgaatgtagc gtggagtaca gagaggtgaa
     4441 agcatgctgt atagcaaagt actcggttcc ctgctcgtac tcgtgggcgt cgcattgctc
     4501 acgctggatg ttttaaaaat acaaagcgtg acgcataatt gacgtttccc tttgacactc
     4561 acgtctattg gcctctgcgt cgttggcgct gttatcgttg cacttttccg ctgggcgcgg
     4621 cgatagcgac ggcacggccg gttctcttaa attagggagc cggctttttt tggtgtcttc
     4681 tcgagacctt gcgaagcaat cttattcgat gagcctctgc ggttattagt ccttcgtttt
     4741 attgcttgac cctgtagtga attttggcat ttatccttgc tactattcgc caaatgcgaa
     4801 tagccatgcc aaaatctact acagggttga cgaaatatga taaaatggac ttagcggaag
     4861 atatgcatac ggaatttacg gctctttcgc gaaggtttga aagcaggata cgagggctca
     4921 ccggacacca ccactcaagc attgataagc attgaataag aagctctcgg cattaggact
     4981 cgaaatctca cctaagcgtc tactttatgt cggtatttga cagttcatta aagttattat
     5041 actgttttct tctcaacctt aattagcaat tcatttatta gaatcaaaat ctaaacatgc
     5101 ctccatttgg ccaatttaca accaaggcga aggaagctat caggaaagcg catgagctgg
     5161 cgatcgagcg cggacagaac cacgtgaacc cgctccatct cctcacggcg ctcatccttc
     5221 aggaggagag catggttacg tcgatcctcg acaagctcga gatcgattcc atccttctta
     5281 ccgacagcct cctcgaagcg gtcgagtcgc cagacgcggc cggcacgctc tcgccttcgt
     5341 atcagattta cctcaccccc gagctcgcgc acgcgattga gaattcctcg aaaatcgcgg
     5401 cgcgtctcaa tgacgagttc gtatcgaccg aacacctctt catcgcgctc atggaagttc
     5461 cgggcaccgc ccgcgagatc ctcgcaaagt tccgcatcga caaggagaag gttctccgcg
     5521 tcctcgagga attgaagagc ggcaagacga ccgacgtttc ccagccgaag aaattcaagg
     5581 cgctcgccaa gtacaccaag agcctcacca aggccgccgc aaacaacaag ctcgatccgg
     5641 ttatcggccg cgatgaggag atttctcgca tcatccagat tctctcgcgc cgcaccaaga
     5701 acaacccgat tcttatcgga gaggctggcg tcggcaagac tgccattgtc gaagggctcg
     5761 cgacgcgcat cgcgaccggc aacgtccccg aatctttgcg cgacaaggag ctcgtctcgc
     5821 tcgacctggg actcctcatc gcgggcacca agtaccgcgg cgagttcgaa gagcgcctta
     5881 agaacatcct gaaggaagtc gaaaagtcgg acggcaaagt gatcctcttc atcgacgaga
     5941 tccacacgat cgtcggcgca gggtcggccg aaggctcgat cgacgccgcc aatatgctca
     6001 aacccgcgct cgcgcgcggc gagcttcgcg cgatcggcgc caccacgttc aaggagtacc
     6061 agaagcacat cgagaaagac ccggcgctca cgcgccgctt ccagccggtc ttcgtgaacg
     6121 agccgtcgat cgacgacgcc atcaccattc tccgcggcct caaggagaag tacgagctct
     6181 accacggcgt acgcattacc gatgacgcca tccgcgcggc ggtcaatctc tcaagccgct
     6241 acattaccga ccgcttcctg cccgacaagg ccgtcgacct gatcgacgaa tcggcatcgc
     6301 acctcaagat ctcgctcgag aacatgccgc ccgcactcca ggaaacccac tcgaggatcg
     6361 tgaagctcga gatcgagcgc gaagccctca agaaggaagt aggggaagac ggcgtcgcga
     6421 aatcggccga aaccaagagc cgcatcaagg atatcgaaaa ggagatcgcc gacctcaagg
     6481 aaaagaccgc cgagctcgaa ctcaagtgga aaaacgagaa ggaaactgtc ggcgacatca
     6541 agtccatcaa gaaggacctc gaagccctgc gcctcgaggc cgagggcgcc gaagcccgcg
     6601 ccgacctctc gctcgcggcg gaaatccgct acggcaagat tcctcagctc cagaaggaat
     6661 atgaactcaa atcgaagcgc ctgaagcgcc tccagtcatc gcgcaggatt ctcaaggagg
     6721 aaatcacgga aaacgatatc gcggacatcg tgtcccgctg gacgggcatt cccgtgtcgc
     6781 gcatgctcga agaggaagca gagaagctcg cccgcatgga cgaggagctc gacaagcgca
     6841 tcatcggcca gaaggaggcg gtcaagaaga tcgccgatac ggtgaagcgg agccgcgcag
     6901 gcatcgccga tccgaacagg ccgatcggct cgttcatctt ccttgggccc accggcgtcg
     6961 gaaagaccga gctcaccaag gcgcttgctg aattcatgtt tgacgacgag aaggcgctta
     7021 tccgcctcga catgtcggaa ttcatggaga agcattcggt ttcgaagatt atcggcgcgc
     7081 ctccgggcta tgtcggccac gaggaaggcg gcaacttcac cgaaaccgtc cgccataggc
     7141 cgtactcggt cattctcttc gacgagatcg aaaaggcgca cccggaagtc ttcaacatcc
     7201 tccttcaggt gctcgacaac ggccgcctga cagacgccaa gggccgcacg gtcaacttcc
     7261 gcaacaccat catcatcctc acctcgaaca tcggcgccca gtacatcgac aagatgcaga
     7321 agatcggctt cgcattgggc gacaaggata agcagaatta cgaggaaatg aaagacaagg
     7381 tcatggaagc gctcaaaaac cacttcaggc cggaattcct gaaccgcctc gacgacattg
     7441 tgctcttcga cgcattgtcg aaggacgcgg tcaaggagat cgtgaagatc cagatcggcc
     7501 tcgtgaagga acgcctcact cagaaggaga tttctctcga agtcactccg gacgcgctcg
     7561 atctgcttgc cgagaagggc tacgatccgc agttcggcgc gcgccccttg aagcgcctca
     7621 tccagaccaa gatcctgaac cccgtcgcgt cgctcattat ctcgaaaggc attgcgaagg
     7681 gcggcatggt ctccgtcggc acgaagaacg gcgagttcac cttcgatgtg aagaagggaa
     7741 ggaggggagc gctcatcgcg gagaacttga tt
//
LOCUS       DASZYS010000039         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_236289, whole genome shotgun sequence.
ACCESSION   DASZYS010000039 DASZYS010000000
VERSION     DASZYS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236289"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..72)
                     /locus_tag="VHF05_02390"
     tRNA            complement(2..72)
                     /locus_tag="VHF05_02390"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Gln,seq:ctg)
     gene            complement(198..539)
                     /locus_tag="VHF05_02395"
     CDS             complement(198..539)
                     /locus_tag="VHF05_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79803.1"
                     /translation="MKTYTLRVRSVDKKIFDALESGAKNVETRAASSKYAGMRAGDAI
                     TFVCGKERLTKAISSAKIYKSLPALLRDYKPSDINPAAKTARELDEMYASFPGYEAKI
                     RRFGIIALELE"
     gene            complement(559..2247)
                     /locus_tag="VHF05_02400"
     CDS             complement(559..2247)
                     /locus_tag="VHF05_02400"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVS79804.1"
                     /translation="MAKRNKHKYIFVVGGVMSGVGKGISTSSIGTALQSKGYKVSLIK
                     ADPYLNVDAGTMNPTEHGEVFVLDSGLETDQDMGNYERFLGTSLPPENYMTNGMVFKT
                     VIDRERALGYKGKCVEPVFHITEEILRRIKKSAEDTNADITIMEIGGTVGEYQNAIFI
                     EAARVLKMKHPKDVLTIMVSYFPVPHMLGEMKTKPAQNAIRQLSSYGVNADIVIARSE
                     FALDQKRKEKMAMATGISADDIISAPDIDSIYEVPVNFENHGLSQRILSHFGIRPKQK
                     DLVEWRKMVSRMKHAEEQLNIAVVGKYFDTGDYVLSDAYISVIEAIKFSTYSQNRKPK
                     LTWINSKHFENGELPLDTLKQFDGVVVPGGFGESGVEGIIRAIQFARENKIPYFGLCY
                     GMQLMTIEYARSVLGWKDATTAEIGGKSKHFVIDIMPEQKKLIEEGKMGGSMRLGGYQ
                     CVLKPGTIAREAYKSAEVRERHRHRYEVNPEFIPALEEGGLVFSGTSPDKRLMEIAEL
                     PRSVHPFFLGTQFHPEFLARPMSPHPLFTEFVKAAIAAHAKTPRKEAKVAVEKR"
     gene            complement(2384..3310)
                     /locus_tag="VHF05_02405"
     CDS             complement(2384..3310)
                     /locus_tag="VHF05_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HVS79805.1"
                     /translation="MIWTNIKRITRSGFFSFWRNGFVSLASVFIMVITLSVIGSIIFV
                     HALLTSSLQALQDKVDVNVYFVTTAPEDQILAMQKDLQSLPDVANVTYVSRDQELADF
                     KERHADNQLTLQALDELGDNPLGAVLNVKAKDPSEYEGIANYLDQRKNAADGSGNIID
                     TVNYSQNKQAIDRLNTIIHSAESLGTILTIVLILLSIIITFNTIRLAIYMAREEIAVM
                     RLVGASRTYIRGPFVVSGILYGLAAGLLTLAIFYPICYWLGNASQNFFIGLNIFDYYI
                     ANFPLIFLVVVGSGMVIGAVSSFLATHKYLKV"
     gene            complement(3425..4105)
                     /gene="ftsE"
                     /locus_tag="VHF05_02410"
     CDS             complement(3425..4105)
                     /gene="ftsE"
                     /locus_tag="VHF05_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HVS79806.1"
                     /translation="MIYFDRVSKQYSDDSVALSDVTFGIEPGEFVSIVGHSGAGKTTL
                     IKMILAEDKPTDGKVYFESTDIHALNKREINQIRRRIGTVFQDFRLLPHKTVYENIAF
                     SMEAAGKTDEEIEADVPHVLELVDLGRKIWNFPNELSGGEKQRVAIARAIVNQPDIIV
                     ADEPTGNLDPINTYEIVQILKKINDLGTIVILTTHNKGIIDSLGKRVITMENGKVIRD
                     DKQGGYVI"
     gene            complement(4241..5116)
                     /locus_tag="VHF05_02415"
     CDS             complement(4241..5116)
                     /locus_tag="VHF05_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129742721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HVS79807.1"
                     /translation="MNAEDKKKRLEELEQSMYSPDFWSNKTKAQATLKEIETLKDELA
                     GVGKYDRGNAVMTIFAGAGGDDAEDFARMLLEMYERFIAGKGWGVSILHENQNDHGGY
                     RSITLDISGKNAYGTLKNESGVHRLVRISPFNAKEQRHTSFAMVEVIPDFEKADELEV
                     PEDEIEIEFARAGGPGGQNVNKRETAVRAVHKPTGLSVHVATERSQAQNKERALQLLK
                     AKLYRRLEDERKAREQGMYVSKSTANEWGSQIRSYVLHPYKLVKDHRTGVEVRDTDAV
                     LDGGLDEFIEAEKDL"
     gene            5341..5646
                     /locus_tag="VHF05_02420"
     CDS             5341..5646
                     /locus_tag="VHF05_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79808.1"
                     /translation="MATSQVNIVISEHNFQPSQLPRCSRCDELRCIHNTTDPETLERI
                     DVAIESQCKNFHGEGETDHVRFEDKHSFLYLNLAAEPFSSDEVFTPPDSVEPSSLLM"
     gene            complement(5722..5794)
                     /locus_tag="VHF05_02425"
     tRNA            complement(5722..5794)
                     /locus_tag="VHF05_02425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5759..5761),aa:Thr,seq:cgt)
BASE COUNT         1401 a         1851 c         1868 g         1649 t
ORIGIN      
        1 gctgcgggac taggactcga acctagatta accgatccag agtcggttgt cctaccatta
       61 gacgatcccg cagcgatata cattattaaa gaaaggcctt tcttaatttc cgagcgagcg
      121 cagccggcaa gctctgctta ccattagacg atcccgcaaa aaaatctgcg ctattcagaa
      181 tactggaatc agcccgatta ttcaagttcc agggcaataa tgccgaaacg gcggattttg
      241 gcctcgtaac cgggaaaact cgcgtacatt tcatccagct cgcgcgccgt ttttgctgcc
      301 ggatttatat ccgaaggctt gtaatcgcgc aaaagggccg ggagcgactt gtagattttg
      361 gcggaagaaa tcgctttcgt gagacgctcc tttccgcaca cgaaggttat cgcgtccccc
      421 gcccgcatcc ccgcgtactt cgaggatgcc gcacgggtct ccacgttctt cgctccgctt
      481 tcgagcgcgt cgaaaatttt cttgtcgacc gaccgaacgc gcagagtgta ggtcttcatg
      541 tccgcgactg cttcggaatt agcgtttttc caccgcgact ttcgcctcct tgcggggcgt
      601 tttcgcatgg gcggcgatcg ccgccttcac gaactcggtg aaaagcgggt gcgggctcat
      661 gggccgggcc agaaattccg gatggaactg cgtgcccagg aagaacggat gcacggagcg
      721 aggaagctcg gcgatctcca tgagccgctt gtcgggcgac gtgccggaga acacgaggcc
      781 gccttcttcg agtgcaggaa tgaactccgg gttcacttca tagcggtgcc tgtggcgctc
      841 gcgcacttcg gcggacttgt acgcttcgcg cgcgatcgtc cccggcttga gtacgcactg
      901 atagccgccg aggcgcatcg acccgcccat cttcccctcc tcgatgagct ttttctgctc
      961 gggcatgatg tcgatgacga agtgcttcga cttgccgccg atctccgcgg tggtcgcgtc
     1021 cttccagccg agaacgctcc gcgcgtactc gatcgtcatg agctgcatcc cgtagcagag
     1081 gccgaaatac ggaatcttgt tctcgcgagc gaactgaatg gcgcggatga ttccctcgac
     1141 gcccgattcg ccgaaaccgc ccggcacgac gacgccatcg aattgcttca gagtatcgag
     1201 cgggagctcg ccgttttcga aatgcttgga attgatccat gtgagcttcg gcttgcgatt
     1261 ctgcgagtag gtcgagaact tgatcgcctc gatgacagaa atgtacgcgt cggagaggac
     1321 atagtcgccc gtatcgaaat actttccgac gaccgcgata ttcaattgct cttccgcgtg
     1381 cttcatccgc gaaaccatct tgcgccattc gacgagatcc ttctgcttcg gacggatgcc
     1441 gaagtgagag agaatgcgct gggaaaggcc gtggttctcg aagttcaccg gcacctcgta
     1501 gatgctgtcg atatcgggcg cggagataat gtcgtcggcg gaaatgccgg tcgccatcgc
     1561 catcttctcc ttgcgcttct ggtcgagggc gaattcgctt cgcgcgatta cgatatcggc
     1621 gttcacgccg tagctcgaga gctggcggat ggcgttctgg gcaggcttag tcttcatctc
     1681 gccgagcatg tgcggcaccg ggaaatacga aaccatgatg gtgaggacgt ccttcgggtg
     1741 cttcatcttg aggacgcgcg ccgcttcgat gaagatggcg ttctggtact cgcccaccgt
     1801 gccgccgatc tccatgatgg tgatgtcggc attcgtgtcc tccgcggatt tcttgatcct
     1861 ccgcaggatt tcctcggtga tgtggaagac cggctcgacg cacttgccct tgtagccgag
     1921 cgcgcgctcg cggtcgatca cggtcttgaa gaccatgccg ttcgtcatgt agttctccgg
     1981 cggaagcgag gtgccgagga agcgctcgta gtttcccatg tcctggtcgg tctcgagtcc
     2041 gctgtcgagc acgaagacct cgccgtgctc ggtcggattc atggttccgg cgtcgacatt
     2101 aagatacggg tcagccttga tgaggctgac cttgtatccc ttcgactgaa gggcggtgcc
     2161 gatggacgaa gtggagatgc ctttgccgac gcccgacatc acgcctccga ctacgaatat
     2221 gtacttgtgc ttattgcgct tcgccatgac tcaaattcaa aaattaaaag ttaaaggtca
     2281 aaattttaat tcataatcca aaaattccga aagcgaaaga gaaactagtg ccattctttc
     2341 ctctctgcgt ttcacttttg gcttttgcat tttggatttt gacttaaacc ttcaggtatt
     2401 tatgagttgc taagaagctc gacactgccc cgattaccat tcccgacccg acgacgacaa
     2461 ggaagatgag cgggaaattc gcgatgtagt aatcgaagat gttcaacccg ataaagaagt
     2521 tctgcgacgc gttgccgagc cagtagcaga ttggatagaa gatcgcgagc gtgaggagcc
     2581 cggcggcgag gccgtagagg atgccggaca ccacgaacgg gccgcggatg taggtgcggg
     2641 aagcgccgac gagccgcatg accgcgatct cctcgcgggc catgtagatc gcgagcctga
     2701 tggtgttgaa ggtgatgatg atggaaagga gaatgaggac gatagtaagg atcgtgccga
     2761 ggctctcggc ggagtggata atcgtgttga gccggtcgat agcctgctta ttttgggaat
     2821 agttgacagt atcgatgata ttgccgctgc cgtcggccgc gttcttgcgc tgatcgaggt
     2881 agttggcgat gccttcgtat tccgaaggat ccttagcctt gacgttcaac acagcgccaa
     2941 gcggattgtc cccgagctcg tcgagggcct ggagagtcag ctggttgtcg gcatggcgct
     3001 ccttgaagtc ggcaagctcc tggtcgcgcg acacgtacgt gacattggcc acgtccggaa
     3061 gcgactgaag gtccttctgc atggcgagga tctggtcttc gggagcggtg gtgacgaagt
     3121 agacattcac gtcgaccttg tcctggagag cctgaagaga ggaagtgagg agagcgtgga
     3181 cgaagatgat cgatccgatg acgctcaagg tgatcaccat gatgaatacc gaggcgagag
     3241 aaacgaaacc gttccgccag aagctgaaga aacccgacct cgttattctt tttatgtttg
     3301 tccagatcat gatactaaat ttccaatttt caatttctaa ttttcaatca atgcttcaat
     3361 gttccaattt cgaagttcat gtcttttgtt tgaaaattga aaattgcaca ttgaaaattt
     3421 atcgtcagat gacgtatcct ccctgcttat cgtcccggat gaccttgccg ttctccatgg
     3481 tgatgacgcg ctttccgaga ctgtcgatga tgcccttgtt gtgcgtggtg aggatcacga
     3541 tggtgccgag atcgttgatc ttcttcagga tctgcacgat ctcgtacgtg ttgatcggat
     3601 cgagattgcc ggtcggctcg tcggccacga tgatgtcggg ctggttgacg atggcgcggg
     3661 caatggcgac gcgctgcttc tcgccgcccg aaagctcgtt cggaaaattc cagatcttgc
     3721 ggccgaggtc gacgagctcc aaaacgtgcg gcacgtcggc ctcgatctct tcgtcggtct
     3781 tccccgccgc ctccatggag aaggcgatgt tttcgtagac ggtcttgtgc ggaaggaggc
     3841 ggaaatcctg gaatacggtg ccgatgcggc ggcggatctg gttgatctcg cgcttgttga
     3901 gcgcatgaat gtcggtcgac tcgaaataga ccttgccgtc ggtcggttta tcctccgcaa
     3961 ggatcatctt gatgagagtg gttttgccgg cgccggagtg gccgacgatg gacacgaatt
     4021 cgcccggctc gatgccgaac gtgacgtcgg aaagcgcgac tgaatcatcc gaatactgct
     4081 tgcttaccct gtcgaaatag atcatttgaa aatcaaaagt aaaaaatcaa aaatgaaaac
     4141 gatttttgac aattacaaaa ctcgctcact atcttgtctt ggttattgta acacgtgcgt
     4201 ccggccggac aaaccttgcc aagcgaacga ttttcaggtg ctacaaatcc ttctcagcct
     4261 cgatgaattc gtcgaggccg ccgtcgagca ccgcatcggt gtcgcggact tcgacgccgg
     4321 tgcggtggtc cttcacgagc ttgtacggat ggaggacgta cgaacggatc tggcttcccc
     4381 attcgtttgc cgtcgatttg gagacgtaca ttccctgctc cctcgccttc cgctcgtctt
     4441 cgaggcggcg gtagagtttc gccttcaaaa gctggagtgc gcgctccttg ttttgtgcct
     4501 ggctgcgctc ggtcgccacg tgcaccgaaa gcccggtcgg cttgtggacg gcgcggacgg
     4561 ctgtttcgcg cttattgacg ttttgccctc caggaccgcc ggcgcgcgcg aactcgatct
     4621 cgatctcgtc ttcgggtacc tcgagttcat ccgctttctc gaagtccgga atgacttcga
     4681 ccatggcaaa cgacgtgtgg cgctgttctt tcgcattaaa gggagagatg cggacgaggc
     4741 ggtggacgcc cgattcgttc ttgagcgttc cgtacgcgtt cttgccgctg atatcgagcg
     4801 tgatgctccg gtagccgccg tgatcattct gattttcatg aaggatggaa acgccccagc
     4861 cttttccggc gataaaacgc tcatacatct cgagaagcat gcgagcgaag tcttccgcgt
     4921 cgtcgccgcc cgcccccgcg aaaatggtca ttaccgcatt gccgcggtcg tatttgccga
     4981 cgcctgcaag ctcgtccttg agcgtctcga tttccttcaa ggtcgcctgg gcctttgtct
     5041 tgttgctcca gaaatcggga ctgtacatcg attgttccag ctcttccagc cgctttttct
     5101 tgtcttccgc attcatcccg ttagagatag gaaacggccg ccgtttccgt cattaattct
     5161 tgatttgtaa gcatatcgac catggacaat atcatctata agtgagattc aataactgaa
     5221 ttatctctaa cgggattcat tgccgctaat tgtatgcgaa aaaagaccgg cgctcaaacg
     5281 cacccgacga gcttgcaata ttcatataga ggagtagaac taagggcaga ggtgtattat
     5341 atggctacaa gccaagtcaa tattgttatt tcagagcaca atttccaacc atcacagctc
     5401 ccacgttgca gtcggtgtga cgaattacga tgtatccaca acaccaccga cccagaaaca
     5461 ctggaaagaa tcgatgtcgc cattgaaagc caatgcaaga attttcatgg cgaaggagaa
     5521 acggaccatg tccgtttcga ggacaagcat tcgtttctct atcttaatct tgccgctgaa
     5581 ccattcagca gtgacgaggt cttcaccccg ccggattctg tcgagccttc ctcgctttta
     5641 atgtaaatcc ttgttccaag tatcgatcgt cgtatcgata caaatttgga tccgtgtgtt
     5701 atcacgggtc ctttttatct gagccacccc cgaggatcga actcgggacc tttgcgttac
     5761 gaatgcaatg ctctaccaac tgagctaggg tggcttaaaa aattccaggt tcggtgctgc
     5821 ataccgatag caggaaggag gtcaaaccgc ctacggctct cgcaaccccg ctcttaccct
     5881 ttccgaacct ggaaaccgta atgctaatac aaacggtgat ttctttcaat atttatgagg
     5941 tatagagagc caagaccgga tcggtcacga ataaggtcgc ggtcacagct cgcacactgc
     6001 tgtgtgcggc cgcgaccccg cctccttcgc ctttgctcag tccggccttg cgaagcgctc
     6061 gcccgcaatc agaattgcgg gttccctaac gcgcttctca cccggctcgt aaaattttct
     6121 actgaaaatt ttatactccg ccgttcgatc ctcggtcgcg gcgtatatac tatttctctc
     6181 gtaaaaacga gagaaatagt tttgatggag tctctattgt actcggctcg aacccatttc
     6241 tccacttcct gaccgcccgc gcctcgcgga ctcggcgcgg cctccctcgc cccgtttccg
     6301 cctcttcata cattttattt ttcgcggggg gggatgcatg ttgcattgaa tggggcggaa
     6361 cggggctcgg tcgggccttg cccttgaggg gcaaggcggc ggctaaaaat acaaatgcga
     6421 agtggtggcc tcggtctgtt ggtttgcttt gtgcgtcttg gccacggagt gcaccactcg
     6481 gccatccggc ctcgggcatt tccgctaggg ctgctcgcgc ctagatgcct aggagcggca
     6541 cttttcctcg ccagtgcgcc gtgctcggcg ctggctcggg tgccgcccct atttgggggg
     6601 aggaaggaat cctcccccca gcttcgggct cctcctcgtt ccttcggagt ccgccctcgc
     6661 acaacccccc acctcgtttt caaaagtgcg tttgtggcaa gattcgtgaa ttcgtattgc
     6721 agacgagcgg cggcgctcac ccgtgggcca ggacgcccgt cgcggaaat
//
LOCUS       DASZYS010000040        16594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_808731, whole genome shotgun sequence.
ACCESSION   DASZYS010000040 DASZYS010000000
VERSION     DASZYS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808731"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VHF05_02430"
     CDS             <1..459
                     /locus_tag="VHF05_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVS79809.1"
                     /translation="TFMLQAQIREEMKKAMLAKDEVRLRTMRGLIAAFTNELLNLKRK
                     PTDELSDDEVITVVKRAVKQRKDSIEQFRAGNREDLAADEEAELAVLEPLLPRMAGHD
                     EIKAVAEKKKTELGIADTSKLGILVGAVMKEFKGNADGAEVKSVVEEILS"
     gene            471..722
                     /locus_tag="VHF05_02435"
     CDS             471..722
                     /locus_tag="VHF05_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79810.1"
                     /translation="MPRSGGIFEGGQGWLNDWENEKDEREEEPANGAKLNGHIKHTAA
                     ELAAEAIKHTNGQSKKEEEEEKKGQQDLFQHESHEKYPD"
     gene            complement(1051..1584)
                     /locus_tag="VHF05_02440"
     CDS             complement(1051..1584)
                     /locus_tag="VHF05_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVS79811.1"
                     /translation="MSLLTRDFFERDALSVARDLIGCYLVRKIGGKREARMIVETEAY
                     IGPEDLASHASKGLTPRTAVMFGPGGVWYVYFIYGMHEMLNIVTGPEGFPSAVLIRGI
                     EGAIGPGRLTKALGITRELNKLPAARKTGLWIEAGEKIPDKLVKRTPRIGVAYAGAEW
                     AGKPYRFVLKNRTGLSR"
     gene            complement(1618..2415)
                     /locus_tag="VHF05_02445"
     CDS             complement(1618..2415)
                     /locus_tag="VHF05_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79812.1"
                     /translation="MKKNRIYILIAVCAVVVVGGFFGIKALLSRNGASAAQFAYVPQA
                     NADPARIESFKVEKPNFVIKATSLSKLEIWAVPAGDDVKESDVKKLADATLASADWSN
                     QTWTAPIPRSKMNLSDVYVVGYDEDNKPVTSASLGVTGADAIQAAIWGNGTTTSNDDI
                     SGPTASEYKALKLGQSGSYGGMTLTLKTIASDTRCNGSNCASRGTVTGTVTASVGDGA
                     PQTLSFDRTKALHFQGYQITVYDVTPEAPNGSKDYSTYVVTFRIQPS"
     gene            complement(2498..3259)
                     /locus_tag="VHF05_02450"
     CDS             complement(2498..3259)
                     /locus_tag="VHF05_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79813.1"
                     /translation="MSKKAILWTIVALLIIVSAGFYYFNTRQSWSGSGILSATASVQS
                     FRVEKPDFVIEGSNLAKVEIWAVPSGTGVTEDEYQKIGDATQSADNPQKWMLPIPSEP
                     ILATDIIARAYDTNGHELGKMSLGIIGATELYNALWATSTEATGTPSTMGTVRTLGVG
                     DSAAWDNLTIKLNSVDSDSCCPEGVDCIWAGNVTATLAITQNGAESTEKIDSTKSLLY
                     KGYAISIESVSPKPTQGEKDFSTYELGLSVKKQSS"
     gene            complement(3300..3722)
                     /locus_tag="VHF05_02455"
     CDS             complement(3300..3722)
                     /locus_tag="VHF05_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79814.1"
                     /translation="MKQEIIDYLESQHTGVLAVKMPDGSLHGATVHFAYSLEPLALFF
                     LTSLSSKKAGAFGSGACEASFVIGTDEIAMKTLQLDGTLESVPADDEKWKEAYLERYP
                     GRKDWSESPEAMRLKFTPRWWRYSDLKDRANATIIASE"
     gene            complement(3729..4109)
                     /locus_tag="VHF05_02460"
     CDS             complement(3729..4109)
                     /locus_tag="VHF05_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVS79815.1"
                     /translation="MNNIAYFEIQASEPAKLAEFYKTAFGWEFKRDELVPIEYYRTQG
                     GSGIFGAILKRPTETPAIGTGTNAYVCSVQVENFDETAKKILDQGGIVAMEKFAVPGR
                     CWQGYFLDIDHNTFGIFQADENAK"
     gene            complement(4123..4815)
                     /locus_tag="VHF05_02465"
     CDS             complement(4123..4815)
                     /locus_tag="VHF05_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79816.1"
                     /translation="MKKTFTIIILLIIVAGLAYALKMHNMAGNVAVNDANSAASSTAD
                     REVKGTVQSVNTDQVAVDGPALVTIKQADGTDAVIAVPSMGINLCAASSSIADVYSLK
                     TGDAVGARGKVGENGQIIPCESPAHYLHLSGGEGVVPASIAVTGTYLCLPHTDTTGLQ
                     TDECALGIQTDDGTYYSLSFGNSQAAFSYKTGDRLNVSGTMTPAEALNSNAWQKYPIK
                     GIISVASTSVAH"
     gene            complement(4952..5569)
                     /locus_tag="VHF05_02470"
     CDS             complement(4952..5569)
                     /locus_tag="VHF05_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79817.1"
                     /translation="MSKKLTILIVVAIILIAAVLAGWKLHFSDNQQTTIVQTATTTTE
                     SDMLTDTQQNNESLTGTVTSTTNGQFIYRNDKIGIQFVFPAGWHIGSDSLNNGTLQLF
                     NYDERMPRKGFSQGMNKIEADINTHNVYFNTEYPEKASSTKQITVAGQKSVQEDLELA
                     TGLKIRAYFIPMPSQPDLYFGIAVYGDPSNFYVLDNLVQSLSWVK"
     gene            complement(5659..6897)
                     /locus_tag="VHF05_02475"
     CDS             complement(5659..6897)
                     /locus_tag="VHF05_02475"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVS79818.1"
                     /translation="MRTAFAVLLSLSLLAVLPVDAQVTVDYQPTPYPLKRNYDGGSMP
                     QDLNIVHLYDGWINNSYNTTLVRDGRLQTGGWGDTYETYIKFDLAGLPVNPTQVVLWL
                     KSFSRGDSSTTVPFGLCELGSPQWDTTLTWLTLPSAVTCAGWFTPPTPGNWWGIDFTP
                     WYAQWENGTVTNNGIATIPQSNNNQFDMFESSRYTFVTNRPILEFTFTPPTGIPNFKM
                     PLPAGYSWLVTTEIGGYDCLDANPTYWPDSAHAGNNYYTIDISASNQKDGGGSYSGSI
                     PVLAAAHGTVVAVGGCSTCDTGYFITLDHGYGYQTRYLHFAQPAARHNGTPFAVGDVV
                     NQGDQLGIMGSTGASSGTHVHVNFWYYGYGRITDTNLSYVVMDGLLLKSYQTECAVNS
                     SGVPTTWIRYYHSSNTPTGN"
     gene            complement(7421..8476)
                     /locus_tag="VHF05_02480"
     CDS             complement(7421..8476)
                     /locus_tag="VHF05_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVS79819.1"
                     /translation="MDQANPEISVHLQTIRGLLAPTGLFLASAHDVSTGYNKAWLRDN
                     FYTSLALEAAGDTETLLKVWRSILEIFRTHEAKINWAIQNKPHASWQYIHARYNPETF
                     EEFWDEWGNKQNDAIGAILWKLGDLHMKNISVIQNDEDRGIVQRLVDYLASIEYWHDP
                     DNGMWEEWEEVHASSVGACVAGLKAVRNAELAAVPDGLIEHGEESLRGLLPRESSQKF
                     CDLALLSLIYPYKVTERGMEDEILKNLEYHLEKEMGIIRYKNDRYYNKNKDGWSEEAE
                     WTFGFPWLSIIYTEREEKEKAKEYLNKARKTVLSDGRIPELFYSNTSTPNENVPLGWS
                     ESLYVVAVAEYAKKFGF"
     gene            complement(8597..8893)
                     /locus_tag="VHF05_02485"
     CDS             complement(8597..8893)
                     /locus_tag="VHF05_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79820.1"
                     /translation="MGIKIENEYDYLVAKAEAETNKAREILLEARERNDYYQEKVAAK
                     LLEFCETQEKSVRLYAEARLYYPDLGDLIEKRWPQYRSLSDEARLKLGELEHGQ"
     gene            complement(9149..9490)
                     /locus_tag="VHF05_02490"
     CDS             complement(9149..9490)
                     /locus_tag="VHF05_02490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HVS79821.1"
                     /translation="MEETNQQNQQNAADASSSATEVARQGSQVTVSYVGKLQNGQVFD
                     QSADHGGSFTFTLGVGQVIAGWDKGLMGAKKGDHLTLTIPPAEAYGAVQGHPLQNETL
                     IFDIDVLDVKN"
     gene            9705..10439
                     /locus_tag="VHF05_02495"
     CDS             9705..10439
                     /locus_tag="VHF05_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HVS79822.1"
                     /translation="MQGEDIKAEGGKEDFPMRLNRFLALKGVASRREADAIIEKKQVA
                     INGRMAGLGAKVNLGDTITLLKGGKGPAEFSYIAYYKPAGVFLDTTSKEWNKSEFARL
                     KPKVWPADLLEKDAEGLVILTNDKRLSRLLNEPEKIERVYVVRTQEDVAPTFLNKLES
                     GIEIGGRLLKARATEPFGERIFSISIAATRKDVEQMCETLRRTVKSIKRERIASLSLK
                     GLQPGAHKKIADKELESFLKKTGLAA"
     gene            10613..11350
                     /locus_tag="VHF05_02500"
     CDS             10613..11350
                     /locus_tag="VHF05_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVS79823.1"
                     /translation="MNDDHTADLPQVNLPSKQLPIKPLTLAELRESEEKREAEINKEF
                     REGFEFLESYDKSVTFFGSARLKPQNEYYLKAQHLAYRIANELGYAVITGGGPGIMEA
                     ANRGAFEAGGESLGMTIRLPHEQVTNPYLTGHENFYYFFSRKVMMTFSAEAYVYFPGG
                     FGTLDEFFEILTLVQTNKIEKVPLILVGDAYWKNLEKFIVDTILTGEMIDPEDLHLFT
                     ITEDEDKIIEMIRNTRVRNGVKYHYDN"
     gene            11560..11841
                     /locus_tag="VHF05_02505"
     CDS             11560..11841
                     /locus_tag="VHF05_02505"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVS79824.1"
                     /translation="MSQTESEELLKEISDGWKLVDNKEIRKEFAFKDFPEALAFVNKV
                     GAIAETEGHHPNIFLHDWNKVDLTLSTHAIGGLSENDFILAAKIDTLRK"
     gene            complement(11930..12313)
                     /locus_tag="VHF05_02510"
     CDS             complement(11930..12313)
                     /locus_tag="VHF05_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HVS79825.1"
                     /translation="MDILLFIGRLLYGAYFLVLGGSYIKNARRIAKREGLAKVPFAPI
                     IIYACALLSIAGGLGVVLGWHTNLALGALIFMLIFTSLRLHDYWNLDEPENISVEMAH
                     FLKNLALIGAALMMYSLITPWPLSI"
     gene            complement(12376..12756)
                     /locus_tag="VHF05_02515"
     CDS             complement(12376..12756)
                     /locus_tag="VHF05_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79826.1"
                     /translation="MANHRTQKNGQKSLQAEIDQILNEVGHVFDEIAEYTFEGDPLHM
                     IDIDKVLSMEMPEVVYKVPARRHQMPVYRNAATEWNFPSRPERVIRAMPKNLQHESKA
                     YEQKLEKEEKKRKALDVLFKKIDY"
     gene            complement(13215..14489)
                     /locus_tag="VHF05_02520"
     CDS             complement(13215..14489)
                     /locus_tag="VHF05_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HVS79827.1"
                     /translation="MFALIDCNNFFVSCERAFQPKLQGVPVVVLSSNDGCVVSRSNEA
                     KELGVPMGVPFFQFKDFFAARNIRVFSSNFRLYSDMSERVMSLLEQFSDLVEIYSVDE
                     AFLQVPKHVAPAEWGKALRETILRSTGIPVSVGIARTKTLAKVAARKAKEEGKRGGPG
                     VFDLEASSEKGAILGSMTAGEIWGIGGASARKLLKCNIKTALDLAKAPDRVIREKLGI
                     GGLKIALELRGVRTLGRSSEEEGKQSIRSTRSFGRGVYAIEELRRSLAIHADRAAAKM
                     RQQGSAATYLSIFIATGKHSEGGRWYGSAMTGFDVPTTDTSEIISRSREMLEEIFRPG
                     LKYAKSGVMLGGFVPENIVPATTLFGGTRHDDRRQIMRLVDAVNGRFGSGALRPLAAL
                     PEKSIAARSQFVSPEYTTSWEGILKVKLVEQC"
     gene            14698..14940
                     /locus_tag="VHF05_02525"
     CDS             14698..14940
                     /locus_tag="VHF05_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79828.1"
                     /translation="MKNKFRFFGSTEKKREVTRVVEQTHGCNGEIHVDNFDVVYYKAG
                     EVIYAKPLRQKVIGPCDGSCGNITATIDDGTGFLYP"
     gene            complement(15031..16164)
                     /locus_tag="VHF05_02530"
     CDS             complement(15031..16164)
                     /locus_tag="VHF05_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HVS79829.1"
                     /translation="MEPLPSKIRPKSLDEYAGQEHVVGSGKPLRKAIEERLLFSFILW
                     GPPGVGKTTLARIYANALNAEFFELSAVSAGKADIQKIVNEPKTLLSKPKILFLDEIH
                     RFNKAQQDFLLPFVESGELMLIGATTENPSFEVIPALLSRCKVFVLKELEPEDMQKIF
                     DRTGIDLSADAREWLITMSNGDARQALSMIENTDKLYGKVTVETLKETLQSSHLRYDK
                     KGEEHYNTISAFIKSMRANQPDAALYYLARMIEAGEDPIFIARRLVIFASEDVGLADP
                     QALILANAVFRAVETVGLPEARINLAHGVAYLSKAPKDKSAYRALEEALGDARKHGNL
                     PIPIKIRNAPTKLMKDLGYGKDYEAYTKDDLLPEKLKGRKYLE"
     gene            complement(16215..>16594)
                     /locus_tag="VHF05_02535"
     CDS             complement(16215..>16594)
                     /locus_tag="VHF05_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVS79830.1"
                     /translation="KMQNFNSKPKTDLKQRCFEFSLKVIRLTDDLPNKRSAWIIADQL
                     IRAACSIGANLVEARGSSSRLEFKKYCEISLKSANETQYWLALLRDTKISSAAFVDPL
                     LKEAEELTKMLAAGVIKLKSRQF"
BASE COUNT         3917 a         4234 c         4244 g         4199 t
ORIGIN      
        1 acattcatgc ttcaggcaca aattcgcgaa gaaatgaaaa aagccatgct cgccaaggac
       61 gaagtgaggc tccgtaccat gcggggactc atcgcggcgt tcaccaacga gctcctgaac
      121 ctcaagcgca agcctacgga cgagctttcc gacgacgaag tcatcacggt cgtgaagcgc
      181 gcagtaaaac agcgcaagga ttcgatcgaa cagttccgtg caggcaaccg cgaggacttg
      241 gcggccgacg aggaagcgga gctcgcggtg ctggaacccc tccttccccg gatggctggg
      301 cacgacgaga tcaaagcggt agccgagaag aaaaagaccg agcttggcat tgctgacacg
      361 tcgaagctcg gcattctcgt gggcgcagtc atgaaggaat tcaaaggcaa tgccgacggc
      421 gccgaagtga agtcagtcgt agaggaaatt ttgtcgtaat ctataaaact atgccacgaa
      481 gcggaggaat ttttgaaggc ggccaaggtt ggttgaacga ttgggagaat gaaaaggacg
      541 aacgcgagga ggaaccagcc aatggcgcca aattaaatgg ccacatcaag cacactgcgg
      601 cagagcttgc cgcagaagcc atcaagcata cgaacggcca gtctaaaaag gaagaagagg
      661 aagaaaagaa aggccagcag gatcttttcc agcacgagtc tcatgaaaaa tacccggatt
      721 aagaatatta tttgactttt tcaagaaata agatattgta ttttaagaaa acacatccat
      781 caggaggatg aatgagcata ctgttagagc gttggattga taacgccgat tcgggagtcg
      841 ttgaacaccc ggaaaaaaga agccctacaa tatatcgatg ctggcagcca ctgttcggaa
      901 tcacatgcgg aaaaatgggt tcacattgaa gcaggcgatt catgaagtgc tggaagccga
      961 cgttccgatt gcggacgacc ataagtgcgt gggaaaacgt atccgcgctt acctgaaaga
     1021 gcatcaggaa tagcgcgctt cggacgggtc ttatctagaa agacccgtcc gattttttaa
     1081 tacgaatcga tacggcttcc ctgcccattc cgctccggcg tatgccacgc cgatccgcgg
     1141 cgtacgcttc acgagcttgt cgggaatttt ctcgcctgct tcgatccaca gtccggtctt
     1201 tctggctgcg ggcaatttgt tcagctcccg cgtgatgcca agcgccttgg tgagcctgcc
     1261 gggcccgatc gctccttcga taccgcggat gagcaccgcc gatggaaagc cttccgggcc
     1321 cgtcacgata ttcagcatct cgtgcattcc gtagatgaag taaacgtacc atactcctcc
     1381 cgggccgaac atgacggcag ttcgcggcgt caatcccttg ctcgcatgcg acgcgagatc
     1441 ttccgggcct atataggctt cggtttcaac gatcatccgc gcttcgcgct tgccgccgat
     1501 cttgcggaca agatagcagc cgatgagatc tcttgcgacc gagagcgcgt cgcgttcaaa
     1561 aaaatcccgg gtaagaagag acatgcgtga actgtaacta gcaactccgc tccactttta
     1621 gctcggctgg atcctgaatg taactacgta ggtcgagtaa tctttcgagc cgttcggagc
     1681 ttccggcgtc acgtcgtata cggtgatctg gtagccttgg aaatggagtg ccttggtgcg
     1741 gtcgaaggaa agcgtctgcg gcgctccgtc gccgacgctc gcggtaacgg tgccggtaac
     1801 ggtgccgcgc gaggcgcagt tggagccgtt gcagcgcgta tcgctcgcga tcgtcttgag
     1861 ggtgagagtc atgccgccgt agcttcccga ttggccaagc ttgagcgcct tgtattcgct
     1921 cgctgtcggt ccgctgatgt cgtcgttcga agtggtcgta ccgttgcccc aaatggctgc
     1981 ctggatcgca tctgcgccgg tgacgccgag agaggcggag gtaacgggct tgttgtcctc
     2041 atcgtatccg accacatata catcgctcaa attcatcttg cttcgaggaa tcggcgcagt
     2101 ccatgtctga ttcgaccagt cggcgctcgc gagggtcgca tcggcgagct tcttgacgtc
     2161 ggattccttg acatcgtcgc ctgccggtac cgcccagatc tcgagttttg aaaggctggt
     2221 cgccttgatg acgaaattcg gcttctccac cttgaatgac tcgatgcgcg ccggatcggc
     2281 attcgcctgg ggaacatagg cgaactgggc tgccgaagcg ccgttgcggc tcaaaagcgc
     2341 cttaatgccg aaaaatccgc ctactacaac gaccgcacag acagctatga gtatgtatat
     2401 cctgttcttt ttcatgggtt ctattctaat ctaattgcca agctttccta ttgccaaatt
     2461 ccttttccta ttatgggaaa tgtacgcctt aaaacgccta agatgattgc tttttgacgc
     2521 taagcccaag ctcgtaggtc gagaagtcct tttcaccctg cgtcggcttc gggcttacgc
     2581 tttcaatcga gatcgcatag cccttgtaca ggagtgattt cgtactgtcg attttctccg
     2641 tcgactcggc gccgttctgc gttatagcaa gcgtcgcagt cacgttgccc gcccagatgc
     2701 agtcgacccc ttccgggcag cagctgtcgg agtcgacgga gttgagcttg atcgtgagat
     2761 tgtcccatgc ggctgaatct cccacgccga gagttctaac agttcccatc gttgaaggcg
     2821 tgcctgtggc ttccgttgaa gttgcccaca gcgcattgta aagctcggtg gcgccgatga
     2881 tgccgaggga cattttgccc agctcgtggc cgttcgtgtc ataggcgcgc gcgatgatat
     2941 cggttgcgag gatcggctcc gatggaatgg ggagcatcca cttttgcggg ttgtcggcgc
     3001 tttgcgtcgc gtcgccgatt ttctgatact cgtcttcggt cactcccgtt cccgacggca
     3061 ccgcccagat ctcgactttc gcgagattcg agccttcgat gacgaaatcg ggtttctcta
     3121 cccggaacga ctggaccgag gcggttgcgg ataggatgcc cgatccgctc cacgactgcc
     3181 gcgtgttgaa gtaataaaag ccggcgctca cgataatgag caaggctact atcgtccaca
     3241 aaattgcttt ctttgacatg cgaatattgt atacgatcaa ccttttccct tctagtccgt
     3301 cattcagacg cgatgatggt cgcgttcgcg cgatccttga ggtcggaata cctccaccac
     3361 cgcggagtaa acttcagtct catggcttcg ggactttccg accaatcctt gcggccgggg
     3421 tatctttcga gataggcttc cttccatttt tcatcatccg cgggaacgga ttcgagcgtg
     3481 ccgtcgagct ggagggtttt cattgctatt tcgtctgtgc caatgacaaa acttgcctcg
     3541 catgcgccgc ttccgaatgc tccggccttc ttcgagctta aggaggttag gaagaaaagc
     3601 gccaaaggtt caagcgagta cgcgaaatgc acggttgcgc catgaagcga cccgtccggc
     3661 attttgacgg ccaagacccc cgtgtgctgg ctttcaagat aatcaataat ttcctgcttc
     3721 atataggatt attttgcatt ctcatcagcc tggaaaatgc cgaaggtgtt gtggtcgata
     3781 tcgaggaagt acccctgcca gcagcggccg ggtacggcga atttttccat agccacgatt
     3841 ccgccttgat cgaggatctt ttttgcggtt tcatcgaagt tctcgacctg caccgagcac
     3901 acgtatgcat tcgtgcccgt accgatcgcg ggcgtttccg tcggccgctt gagaatcgcg
     3961 ccgaagatgc ccgacccgcc ctgcgtgcga tagtattcga tcgggacaag ctcgtccctc
     4021 ttgaactccc agccgaaagc ggttttgtaa aactcggcaa gctttgcagg ctcggatgcc
     4081 tggatttcaa aatatgcgat gttgttcatg ataatgattg acttaatgtg caaccgacgt
     4141 gctcgccacc gagataatgc ccttgatcgg gtatttctgc cacgcattag aattgagcgc
     4201 ctctgcgggc gtcatcgtgc cggaaacatt caagcggtct cccgtcttgt acgaaaacgc
     4261 ggcttgggaa ttgccgaaac tgagcgaata atacgtgccg tcatcagtct gaatgccaag
     4321 cgcgcattca tcagtctgca gacctgtcgt gtctgtatgc ggtaggcaaa gatatgtacc
     4381 cgtaaccgca atgcttgccg gtactactcc ttcgccgccc gaaaggtgca aataatgggc
     4441 tggtgattcg cacggtatga tctggccgtt ttcgccgacc tttccgcgcg cccccactgc
     4501 gtcgcccgtt ttgagcgagt aaacatcggc gatgctcgag cttgcggcgc aaagattgat
     4561 gcccatcgaa ggcacggcga ttacagcgtc agtcccgtcc gcctgtttga tcgtaacaag
     4621 cgcagggccg tccaccgcca cttgatcggt atttaccgac tggactgtgc ccttaacttc
     4681 cctgtcggct gttgaggacg cggcgctatt ggcgtcgttt actgccacgt tgccggccat
     4741 attatgcatc ttcaaggcat atgcaagccc tgcaacaatg ataagaagaa tgatgattgt
     4801 gaatgtcttt ttcatgcaaa aattatagca tatgggcttt tctgcatgat cgagaacacg
     4861 cttttccctt gacacatggt tgtctacaaa aaagagaagg tctaataaga ccccaagctg
     4921 gttcttgagg taagccgaca atattccatt cctatttgac ccaactcaaa ctttgaacga
     4981 gattgtctaa aacgtagaaa tttgaaggat cgccgtatac tgcaattcca aaatacaagt
     5041 cgggctgcga tggcatagga atgaaataag cccggatctt aagacctgtc gcaagttcca
     5101 aatcttcctg tacagatttt tgcccagcca ccgtgatttg ctttgtcgaa ctggcttttt
     5161 ctggatattc ggtgttgaaa taaacgttat gtgtatttat atctgcttca attttattca
     5221 ttccctggct gaatccttta cggggcattc tttcgtcgta attaaaaagt tggagggttc
     5281 cgttattaag cgaatcactt ccaatgtgcc atccagctgg aaaaacaaat tgtatcccga
     5341 ttttatcgtt tctgtatata aattgaccat tggtagttga tgtaaccgtg cctgtaaggg
     5401 attcattatt ttgctgcgta tctgtcagca tatctgattc cgttgtagtg gttgcagttt
     5461 gtacaatagt cgtttgctga ttgtcagaaa aatggagttt ccatccggcc aaaacagcag
     5521 caataagtat aatggccact actatgagaa ttgtcagttt tttgctcatg ataattttga
     5581 atatagcacg gccttgtttt cttgtgaaat aaaatcggca gggtgagaat cccctgccga
     5641 atcagaaaag aagaaacttt aattcccagt tggagtattg ctggaatgat agtaacgaat
     5701 ccaagtagtt ggaactccgc ttgaatttac agcacactct gtttggtaac tttttaaaag
     5761 taacccatcc atcaccacat aactaagatt cgtgtcagtg attcgaccgt aaccgtagta
     5821 ccagaagttt acatggacgt gtgtgcctga actagccccg gtacttccca taattccaag
     5881 ctgatctcct tgattaacta catcaccaac tgcaaacggt gtgccattgt gtcttgctgc
     5941 tggttgggca aaatgtaagt agcgtgtttg atatccatac ccgtgatcca aggtaataaa
     6001 gtaacctgtg tcacaagtgg aacagcctcc tactgctact actgttccgt gagcagcggc
     6061 aagtactgga atagatcctg aataactgcc tccgccgtcc ttttggttac ttgcagaaat
     6121 atctatcgtg taataattat ttcctgcatg cgcgctatct ggccaataag ttggattggc
     6181 atccaagcag tcgtaaccgc caatctcggt tgttacgagc caggaatacc ccgctggcaa
     6241 aggcatcttg aaattcggta ttcccgttgg aggagtaaat gtaaattcca agataggccg
     6301 attagttacg aaggtgtacc ttgagctttc aaacatatca aattgattat tgttgctctg
     6361 aggtattgtc gcaatgccgt tatttgtaac cgtcccattt tcccattggg cataccaggg
     6421 tgtaaaatcg attccccacc aattacccgg tgtcggcggt gtaaaccatc ctgcacaagt
     6481 aacagctgaa ggcaatgtta gccaagtcaa tgttgtgtcc cactgaggac ttccgagttc
     6541 acaaagacca aacggaacgg ttgttgagct atctccgcga gaaaaggatt ttaaccataa
     6601 tactacttgc gttggattaa caggcaagcc tgccaaatcg aactttatat atgtctcgta
     6661 tgtgtcaccc catccacccg tctgaagtct gccatctcgc acaagcgtgg tgttgtatga
     6721 gttgttgatc caaccatcat aaagatggac aatatttaga tcttggggca ttgaaccacc
     6781 atcataattc ctttttaagg gatacggtgt tggttgataa tcgacagtta cttgggcgtc
     6841 cacaggaaga acggccagaa gagacaagga tagaagaact gcaaatgctg ttctcattta
     6901 gagtttcctt tttctaaaga acattccgct ttgtgagcgg agtatgcata cttaaatttc
     6961 aagtacacaa aactgatgta aaataatatc aaaattttta agctctgcaa tagaagatca
     7021 aattgaaaaa tctaccgtgg aaaatagaaa ttgactaaat tgtccatatg tgtaatctat
     7081 gtcttagagg taatacaacg tgaagaaaat agtggcggaa attcgggata atccgatcaa
     7141 gtcggtgctc ctggccgtgt tatatgtatt tagctgtcga gttgtatatc ttgttttgag
     7201 agattttgcg cctggctgga gtgtagccct agcgtgcatc ggcggattag tgctcggtct
     7261 ttttggtatt gcgtatgcag tcgaaccgat aatgaaattc ttagatgcca aggttgatgc
     7321 tttgtttagg atcagaaggc actaaccgag cagggtggag tgtttgagca tcgaatgcaa
     7381 attcggtgct cattttttat ttaggatatt aactgagaaa ctagaaccca aactttttcg
     7441 catattcggc gacagcgacg acatagaggc tctcggacca gccgagggga acgttctcgt
     7501 tgggagtcga agtgttcgag taaaacaatt cgggaatgcg gccgtcggaa agaactgtct
     7561 ttcgcgcctt attgagatat tccttcgctt tctccttctc ttccctttcg gtgtaaatga
     7621 tcgaaagcca cggaaagccg aacgtccatt cagcctcttc gctccagccg tccttgtttt
     7681 tgttgtagta gcgatcgttc ttgtagcgga tgatgcccat ctccttctcg agatgatatt
     7741 cgagattctt gaggatttcg tcttccatgc cgcgctctgt cactttgtac ggatagatga
     7801 gcgagagaag cgccaaatcg cagaacttct gcgacgactc tctcggcaga agcccgcgaa
     7861 gcgattcctc cccatgctcg ataagtccat cgggaaccgc cgcgagctcc gcattgcgca
     7921 cggctttgag gccggcgacg caggcgccta ctgacgatgc gtgcacctcc tcccattctt
     7981 cccacatgcc gttgtcggga tcgtgccaat attctatgga agcaaggtag tcgacgagcc
     8041 tctgcacgat tcccctatcc tcatcgttct ggatgaccga aatatttttc atgtgcaggt
     8101 cgccgagctt ccagaggatc gcgccgatgg cgtcgttctg cttgttgccc cattcgtccc
     8161 agaattcttc gaaggtttcg gggttgtagc gggcatggat gtactgccag ctcgcatgcg
     8221 gcttgttttg aatcgcccag tttatcttcg cctcgtgcgt gcggaatatt tccaaaatcg
     8281 agcgccacac cttgagaagc gtctccgtgt cgccagccgc ctcgagggcg agggatgtat
     8341 agaaattgtc gcgaagccac gctttgttgt agccggtgga gacgtcgtgc gccgatgcga
     8401 gaaaaagtcc ggtcggagcg aggaggcccc gtatcgtttg gaggtggacg gaaatttcag
     8461 ggttagcctg atccatcccg tttagaagca gatcacgctc accgcgtttt ctgcattcgc
     8521 ataaaatctt ttaataagta tttcgaccat tgtaatcatc atcaagtgat cgcggcttct
     8581 aacgggatcc atagatttat tgtccatgct ctaactctcc gagcttgagc ctcgcttcgt
     8641 cgcttagcga gcggtactgc ggccagcgct tctcgataag atcgcccagg tcgggataat
     8701 aaagccgcgc ttcggcgtac aggcgcacag atttctcctg agtctcgcag aattcgagaa
     8761 gcttcgcggc gactttctcc tgataataat cattgcgctc gcgggcctcc agaagaattt
     8821 cccgagcttt gttggtttcc gcttcagcct tcgctaccag atagtcgtat tcgttctcaa
     8881 ttttgattcc catagttctt agcgttcgtt ctggggcgtg tattctgccg gcactttcat
     8941 tccggatact ctcgcgtcga aatccaaaca aactttcctg cacgcgtctc tcgttttttc
     9001 gaacgatgca cccatcacct caacgctcgc ctcgctttcg gatgccggat tttcaaattt
     9061 aattccttcc atgctttcag tatactatgg cgctgtaaat gaaaaaaccc gcgcggtgag
     9121 cgggtttttg tggatttctc aggacttcct agtttttcac gtcgaggacg tcgatatcga
     9181 agataagcgt ctcattctga agcggatgac cttgcaccgc gccgtacgct tcggcgggcg
     9241 ggatggtgag cgtcaggtgg tcgcccttct tggcgcccat gaggcccttg tcccagccgg
     9301 caatcacctg gccgacgccc aaagtgaaag tgaacgagcc gccgtggtcg gccgactggt
     9361 cgaagacctg gccgttctgg agcttgccga catagctaac cgtcacctgc gagccctggc
     9421 gggccacttc ggtcgccgat gaagacgcat cggctgcatt ctgctgattc tgctgattag
     9481 tttcttccat tgttgcttgg cttggcgtca tgtccggctt gaagaatgac cctataagac
     9541 ctcccaggag gaaataagcg accactacga ttgcaacaga taccgcgacc cattcgtttc
     9601 gtgtaagcct tcgcatgagc aaacaaatta atttctaatg ctacgaatta tagcttcaat
     9661 atgcaaattg aaaaaggctc aggatagcgt atagtggaga aactatgcaa ggcgaagata
     9721 tcaaggcgga aggcggcaaa gaggattttc ccatgcggct caaccgcttc ctggcgctca
     9781 aaggcgtggc gagccgccgc gaggctgatg caattatcga aaagaagcag gttgccataa
     9841 acggccgcat ggccggtctt ggcgcgaagg tgaacctcgg cgacaccatt acccttctta
     9901 agggcggcaa aggaccggcc gagttttcct acatcgccta ctacaagccc gcaggcgtct
     9961 ttctcgacac cacttccaaa gagtggaaca agtcggaatt cgcgcggctt aagccgaagg
    10021 tctggcccgc ggatctcctc gagaaggatg cggaagggct cgtcatcctc acgaacgaca
    10081 agcgcctctc gcgcctcttg aacgagccgg agaaaattga gcgcgtatac gtcgtccgca
    10141 cccaggagga tgtggcgccc acgttcctca acaagctcga gagcggaatc gagatcggcg
    10201 gccgcctcct caaggcccgc gcgacggagc ctttcggaga gaggattttt tccatctcca
    10261 tcgctgcgac ccgcaaggac gtggagcaaa tgtgcgaaac gctccgccgc accgtgaagt
    10321 cgatcaagcg cgagcgcatc gcctcccttt ccctcaaggg cctccagccc ggcgcacaca
    10381 agaagatcgc cgacaaggaa ctcgagagct ttttgaaaaa gaccggcttg gcggcttagc
    10441 ggaaattttt agctgtgttt tgaatagtga atgtctgata caatattcct atgaatcccg
    10501 ttagaagccg cggtcgctaa cgggtggatg aatttagggt cgcgttagaa cttaactagc
    10561 taaagtagaa gcgaacttat cttgagtatg atcgcgatct gcttctaaca ggatgaatga
    10621 tgaccatact gccgacctcc cgcaggtcaa tttgccttcg aagcaattgc ccatcaagcc
    10681 cctcaccctc gcggagctcc gcgagtccga ggagaagcgc gaagcggaga tcaacaagga
    10741 attcagggag ggattcgaat tcttggagag ctatgacaaa tcagtgacat tcttcggctc
    10801 ggcgcgcctc aagccccaga acgagtacta tcttaaggct cagcatctcg cttatcgcat
    10861 cgcgaacgaa ctggggtacg ccgtcattac cggcggcggc cccggcatca tggaagctgc
    10921 gaaccgcggc gcatttgagg ccggcggaga gtcgctcggc atgacgatcc gccttcccca
    10981 cgagcaagtt acgaacccct atctcacggg gcacgagaat ttctattatt ttttcagccg
    11041 caaggtcatg atgaccttct cggccgaagc ctatgtttac ttccccggcg gattcggcac
    11101 cctcgacgaa ttcttcgaga tcctcacgct cgtccagacc aataagatcg aaaaagtgcc
    11161 gcttatcctc gtcggcgatg cgtattggaa aaatctcgag aaattcatcg tcgacaccat
    11221 tctcaccgga gaaatgatcg atcctgagga tcttcacctc ttcaccatta ccgaagatga
    11281 agataaaatc atcgaaatga tccgcaatac ccgtgtcagg aacggcgtca agtatcatta
    11341 cgataattag cgttctgtca ttgcgaggag tccctgcctg tcgcaggcgg ttcgacgaag
    11401 caatctcaac ctactagcct tggattcccc gctgggaatg acaaagggca acggattgct
    11461 tcgcctgcgc ggacttggct cacaatgaca aaaagaaaca tgaatataat tcgtcttacc
    11521 gataaaaagt gcgttccatg cgaaggtggt ataccgccca tgagtcagac tgaatccgaa
    11581 gaactcctta aggaaatctc cgacggctgg aagcttgtcg ataataagga gatccggaag
    11641 gagtttgcct tcaaggattt tcccgaggca ctggcatttg taaacaaggt cggggctatc
    11701 gcggaaaccg aaggtcatca cccgaatatc ttcctccacg actggaacaa ggtcgatctg
    11761 accctcagta cccatgccat cggcggtctt tccgaaaacg atttcattct tgcggcgaag
    11821 atagatacac tgcgaaaata atgagtagat agtagtgagt acagagaaaa gacgcgccga
    11881 acggtgcgtc ttgttttatc tatctaccac ttactttcta ctcagtactc tatatcgaca
    11941 gcggccaggg cgtgatgagc gagtacatca tgagcgcggc gccgataaga gccaggttct
    12001 tgagaaaatg cgccatttcg accgagatgt tctcgggttc gtcgagattc caatagtcgt
    12061 ggaggcggag cgaggtgaaa atgagcatga agatgagcgc gccgagcgcg agatttgtgt
    12121 gccagccgag tacgacgccg agacctcccg caatcgagag aagcgcgcat gcatagatga
    12181 ttattggagc gaacgggacc ttcgcaagcc cttcgcgctt cgcgatgcgt cgggcattct
    12241 tgatgtaact gccgccaaga accagaaaat atgctccata gagcaaccgg ccgataaaaa
    12301 gcaaaatatc catggggttc attatacctc atggatattt gcgttcttat tttctattca
    12361 caccttccga tatctttaat aatcgatttt cttgaaaagc acgtcgagag ccttccgctt
    12421 cttttcttct ttttcgagct tttgttcgta ggccttcgat tcatgctgaa ggtttttcgg
    12481 catcgcgcgg attacgcgct ctggtcggct ggggaaattc cattcggttg cagcgtttcg
    12541 gtataccggc atctggtgcc tccgtgcagg tactttatac acgacttccg gcatttccat
    12601 ggaaagcacc ttatcgatat cgatcatgtg aagcgggtcg ccttcaaagg tgtattctgc
    12661 gatctcgtcg aacacatgac cgacttcgtt gagaatctga tcgatctcag cctgcaggct
    12721 tttctggccg tttttctggg ttcggtggtt tgccatatgc gtaataatta ggatgctaat
    12781 tatatagcat atttatttat aaaagtcaaa ttaaatggcc ccgatgatta tttcatcgga
    12841 gccaaggggc tttctatgca aacaaggcgg caaattggcc tttaagcgag cttttctcga
    12901 gatttttctc aagttttttt cgcattgctg aaggcgagac gaactcgccg ggtgcccgtg
    12961 gaatcggacg ccgttttggc ggtgttttta cgattctcaa cctacggctc gctcttgcat
    13021 tattgaaaag gtgcattttc tctcctattg gaaatgtgcc gcaacatgta tatgagtcat
    13081 gagcagcgcg aaaaaaacgt tcgtacattt tctacgcgct gctcatgctt ttgcaaatgc
    13141 tcccttccgt acttctaggt cggaaacccg ctaaatccct ctagtatcta tggtggctct
    13201 gcggtaggaa aaagtcaaca ctgttctacc agcttcactt taagaattcc ttcccaactg
    13261 gtagtgtatt cgggagaaac gaactggctt cgcgccgcta tcgatttctc cggcaatgcc
    13321 gcgagcggac ggagcgcgcc ggagccgaaa cgtccattca ccgcgtccac gagcctcatg
    13381 atctgccggc ggtcgtcgtg gcgcgttccg ccgaagaggg ttgtggccgg cacgatattc
    13441 tcgggcacga agcctcccag catgactccc gacttggcgt atttgagccc ggggcggaaa
    13501 atctcctcaa gcatttcccg tgaacgcgaa atgatctcgc tggtatcagt cgttggcaca
    13561 tcgaaacccg tcatggcgga gccgtaccat cgtccgcctt cggagtgctt gccggtggcg
    13621 atgaagatcg agagataggt cgcggcgctt ccctgctgcc gcatttttgc agcggcgcga
    13681 tccgcgtgga tcgcgagcga ccgccgaagc tcttcgatcg cgtatactcc tcggccgaag
    13741 gaacgagtcg atcggatcga ctgcttccct tcttcctcag agctgcggcc gagcgtgcga
    13801 acgccgcgga gctccaaggc gatcttgagt cctccgatgc cgagtttttc cctgatgact
    13861 ctgtcgggag ctttcgcgag atcgagcgcc gtttttatat tgcatttgag gagcttgcgt
    13921 gcggaagcgc caccgatgcc ccatatctcg cctgccgtca ttgagccgag gatcgctccc
    13981 ttctctgagg acgcttcgag atcgaatact cccggtccgc cgcgttttcc ttcttccttc
    14041 gccttgcgcg cggcgacttt cgcgagcgtt ttggtgcgcg cgataccgac ggaaaccgga
    14101 atgccagtag agcgcaggat cgtctctctc aatgcctttc cccattccgc gggtgcgaca
    14161 tgcttgggta cctggagaaa tgcttcatcg acggagtata tttctactaa atccgaaaat
    14221 tgctcgagca ggctcatgac ccgctcggac atgtcggaat agaggcggaa gttcgaggaa
    14281 aagacgcgga tgttccgcgc ggcgaaaaaa tccttgaact ggaagaacgg tacgcccatc
    14341 ggaaccccga gctccttcgc ttcgttcgat cgcgacacga cgcagccgtc gttcgacgat
    14401 agcaccacga ccggcacacc ctggagcttc ggctgaaacg cccgctcgca ggaaacgaag
    14461 aaattgttgc agtcaatcag ggcgaacatt gggaaacttt tagcttttag gaaaaatctt
    14521 ttctgtcatg ccgagaacgg catccaagac ttcaggatta tctgctcata agaccggatc
    14581 acgtttctct gctgaggaca aggctcacca taatgaccaa agacattcaa aatcaagctt
    14641 tttaagcata cttgtgtttt attaaaatat atgttattat gaagtaagag gtctagaatg
    14701 aagaataaat ttcgtttttt tggttcaaca gagaaaaaaa gagaggtaac tagagttgtc
    14761 gaacagacac atgggtgtaa tggtgagatt cacgtcgaca atttcgatgt tgtctactac
    14821 aaagctgggg aggtcatcta cgctaaacca ctgcggcaaa aagtgattgg tccttgtgac
    14881 ggaagttgcg gcaatattac tgcaaccatc gacgatggca caggtttcct ttatccttaa
    14941 attcgatttt caccgctcgc tccagaacac cggagcgagc ggtttctttc ttctactggt
    15001 gaagctagag aagctagcaa agctaataac ctactccaga tactttcgac ctttgagctt
    15061 ttctggcaga agatcatcct tcgtgtacgc ttcgtaatcc ttgccgtatc cgagatcttt
    15121 cataagcttt gtcggagcgt tacggatttt tatgggaatg ggaagattgc cgtgcttgcg
    15181 tgcgtcccca agtgcctcct cgagcgcgcg atacgcgctc ttgtctttgg gagctttcga
    15241 gagatatgcg actccgtgcg cgagattgat acgcgcttcg ggaagaccga ccgtctccac
    15301 cgccctaaag accgcgttcg cgagaatgag cgcctgcggg tcggcgagcc ctacgtcctc
    15361 gctcgcgaaa atgacgagcc tgcgggcgat gaagatcggg tcttctccag cttcgatcat
    15421 ccgggcgaga tagtagagcg cggcatcagg ctggttcgcc cgcatcgact tgatgaacgc
    15481 gcttatggtg ttgtagtgct cctcgccttt cttgtcgtag cggaggtgcg aggattggag
    15541 cgtttccttg agagtttcaa cagtaacctt gccatagagc ttatccgtgt tctcaatcat
    15601 tgagagggcc tggcgcgcgt cgccattact catagtgatg agccattcgc gcgcatcagc
    15661 gctgagatcg atgccggtgc ggtcgaaaat cttctgcatg tcttccggct cgagttcttt
    15721 gagcacgaac accttgcagc gcgacaggag cgccggaatg acttcgaacg aaggattctc
    15781 ggtcgtcgcg ccgatgagca tcagttcgcc ggactcgacg aagggcagaa ggaaatcctg
    15841 ctgggctttg ttgaaacgat ggatctcatc gaggaataaa atcttcggct tgcttaagag
    15901 agtcttcggc tcgtttacta ttttttggat gtccgctttt ccggcggaaa cggccgagag
    15961 ctcgaaaaat tccgcgttaa gcgcattcgc gtagatacga gcgagcgtgg ttttccctac
    16021 cccgggcggc ccccagagaa taaaggaaaa gagcaggcgc tcttcaatcg cctttctgag
    16081 aggcttcccg gagcccacaa cgtgctcctg gccggcatat tcatcgaggc ttttgggacg
    16141 gattttagaa ggcagaggtt ccataaagag aagttaaaat tcaaaatcca aaaggcagaa
    16201 gtataatttg taagctaaaa ttgcctgctt tttaatttta tcaccccagc cgcaagcatt
    16261 ttcgttagtt cctccgcttc ttttagcaaa ggatccacga atgcagcaga agaaattttc
    16321 gtatcccgaa gcagcgctaa ccagtactgc gtttcattgg cacttttaag agagatttca
    16381 caatattttt taaattccag tcgagagctt gacccccgag cttctacaag attggcaccg
    16441 atcgaacaag ctgctctaat aagttggtct gcaattatcc aagcacttct tttgttaggt
    16501 aaatcgtctg ttaatcgaat aacctttagg ctgaactcaa agcatctttg ttttaaatca
    16561 gttttgggtt tggaattaaa attttgcatt ttga
//
LOCUS       DASZYS010000041         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1261501, whole genome shotgun sequence.
ACCESSION   DASZYS010000041 DASZYS010000000
VERSION     DASZYS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261501"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHF05_02540"
     CDS             <1..230
                     /locus_tag="VHF05_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVS79831.1"
                     /translation="KNKSRKTIYVWAFEGDCDPQNIISNTFSMEWPPHSGKQQEFPEN
                     DRANFFSYEEAVKKILPAQIMFLDQLREKLR"
     gene            227..1867
                     /locus_tag="VHF05_02545"
     CDS             227..1867
                     /locus_tag="VHF05_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS79832.1"
                     /translation="MTQREALDILKTGANVFLTGAAGSGKTYVLNSYIDYLRNNGVDV
                     GITASTGIAATHLGGVTIHSWTGLGVRDSLTPYDLEALEEKQYLWKRFESAKVLVIDE
                     ISMLHHFRLDLVDRICRSFKRTDAPFGGLQIVLCGDFFQLPPVARMGEEALSAYHSDA
                     WANMGIHVCYLEEQWRQKDDEMIRILRAIRGNDVTDDVYESLRARDNAALPRGIQPTR
                     LYTHNIDVDRINDQHLSELEGETHEYRMRSKGNPALVEALKKSCLAPEVLTLKTGTRV
                     MFVKNNFDAGYVNGTLGVVTGFDDMGMPVVKTLPGETITADEMTWSVEEDGKIKAEVT
                     QVPLRLAWAITVHKSQGMSLDAVEVDLSKSFVAGMGYVALSRVRTLAGLRLLGINNQA
                     LMINPEVLEFDRELLEASKKQEEWIAGISPAEKKKLHEEFIVRIGGTPGKKLEKKTKV
                     PTIEVTRQFLLEKLSLETIAAKRELTVETIVSHIEKLREKDPALDIEHIRPHKIIIEK
                     VKDAFKKAGDTRLTSAKNILGEKISFLEIRIARLFLDS"
     gene            complement(1871..3016)
                     /locus_tag="VHF05_02550"
     CDS             complement(1871..3016)
                     /locus_tag="VHF05_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HVS79833.1"
                     /translation="MNTGAYPFGLSVFFPARNEASNIEHAVMEAEAVLHGLFRKDDYE
                     IIIVDDGSIDGTGEIAESIAARESRVRVIHHSENLGYGAALKTGILAAQKEFVFWSDA
                     DMQFDMRDLKKLLPYAESRNAVLGFRESRKDPFIRKIYARGWNILNRILFDLSVRDID
                     CAFKLFRRETLLSIPVYSNGAMASAEILMRLKRNGVSFAEVPVGHRERAAGRASGAKP
                     SVVFRAFREMMQIYWGDHERGGNDAASQAMRFASVGLLNTAVDFFSYVYMTRLIPFFA
                     ARIILAKIVSFGFGTATSLKFNHSWTFGMKGKLTLAEAARFYSVAGISLVVNAISMYV
                     FADLLHLNDIIAAGLSTVATFSWSFIASRLWVFTGTSAHPRRLALEQ"
     gene            complement(3077..4150)
                     /locus_tag="VHF05_02555"
     CDS             complement(3077..4150)
                     /locus_tag="VHF05_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79834.1"
                     /translation="MKETVHTRTKILILIALGLILAAVALAGRFLAHPETSRGGALSF
                     SALEERNPGAAGQKPKVAAIEWKAPGSFDTKKKLDDYFSSAKASGFTDIYIRIDDALD
                     FKSIRDRNEERKKDEFDASLRDFVSEASEYGIRVEGLGGAPEWILPKNLDDVEYLIDY
                     VAAYNAAADSDQKLGGMVFDIEPQALDDFDSDSGQQELLSDFVEMIDESARHIRSAAP
                     DLTLGFTVPFWFDGATRDIPNVPYAGSSAPTVFQAIRALSQTAHGEILVMAYRNKSSG
                     HNGAIELSSDEINFANNYAPNVRILVGIAANKTSPSSITFYGKKQRDVQSDAENIVSA
                     LGASPSFGGLFIHDFESYEDLKK"
     gene            complement(4155..5504)
                     /locus_tag="VHF05_02560"
     CDS             complement(4155..5504)
                     /locus_tag="VHF05_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVS79835.1"
                     /translation="MDARTHIILENVKNIVLSLLVIAAFAGLVIYFYKNMFAPHVFFD
                     STADRILFLTAATDGLIFVLSLFRREEKHGKLFCDPEKLTVLIVCHNGEVLIGETIKQ
                     ALVHVPPKHIIVISDASTDDTAEVARSFGVRVFENEESLNKSMSINFYASHIETPYTL
                     ILDDDTHIGHTVIPTNLLDEDYSAVAFNVMPAVRPGLLNKLQRFEYRKSMYFGKRQRS
                     DSGSIGNVSGAIGLFHTKDLIAQSHKHSGQYGGEDQQRTLLLHLEGSHKKGVTFCDTL
                     VETEAPATAAKLFKQRATKWNCSVNELFILNMRVVFHSNTHFLLKTERAYKLFIFGTE
                     PIRILAAFFSIFHPENILLLYAFYFSIEVAAWSRMGRKDHPWILPLVPGYNLLKVKAG
                     FIAYFWWFKMKYDYVVRKKFYQFVTGRNLPREYATTATLILALSGYAIFKFIIFLSA"
BASE COUNT         1267 a         1509 c         1625 g         1197 t
ORIGIN      
        1 gaaaaaacaa atcgcgaaaa acgatctacg tctgggcttt tgaaggcgat tgcgatccgc
       61 aaaatataat tagcaataca ttcagcatgg aatggccgcc tcattccgga aaacagcaag
      121 aatttcccga aaatgaccgt gccaactttt tctcctatga agaggcggtt aaaaaaatcc
      181 tccctgcaca gatcatgttc ctcgaccaat taagggaaaa actgcgatga cccagcgcga
      241 agccctcgac atactgaaaa ccggagcgaa cgtctttctc acgggagcgg cgggaagcgg
      301 caaaacctac gtgctgaatt cctatatcga ttatctgcgc aataacggcg tggatgtcgg
      361 catcacggct tcgaccggca ttgccgcgac gcacttgggc ggcgtcacca tccattcctg
      421 gacaggtctc ggcgtgcgcg actcgctcac tccctatgac ctcgaagcgc tcgaggagaa
      481 gcaatatctc tggaagcgat tcgaatcggc aaaggtgctt gttatcgacg agatatcgat
      541 gctccaccat ttccggctcg atctcgtcga ccgcatctgc cggtcgttca agcgcaccga
      601 cgcgcccttc ggcggccttc agatcgtgct ttgcggcgac ttcttccagc tgccgccggt
      661 cgcgcggatg ggcgaggagg cgctttcggc ctatcattcc gacgcatggg cgaacatggg
      721 catccatgtc tgctatctcg aagagcagtg gcggcagaaa gacgacgaga tgatccggat
      781 tctccgcgcc atccgcggaa acgacgtgac ggatgacgtc tacgagagcc tccgcgcgcg
      841 cgacaatgcg gcattgccgc gcggcatcca gccgacgcgc ctctacacgc acaatatcga
      901 cgtcgatcgg atcaacgacc agcatctgtc ggagctcgaa ggcgaaacgc acgagtatcg
      961 catgcggtcg aaggggaatc cggcgctcgt cgaggcgctc aagaaaagct gtcttgctcc
     1021 cgaggttctc accctcaaga ccggcacgcg cgtcatgttc gtgaaaaaca atttcgacgc
     1081 cggatacgtc aacggaacgc tcggggtcgt taccggcttc gacgatatgg ggatgccggt
     1141 cgtcaaaacc ctccctggcg agacgattac cgcggacgaa atgacctgga gcgtcgagga
     1201 agacggcaag ataaaagccg aagttaccca ggtgccgctc cgcctcgcct gggccattac
     1261 agtgcacaag agccagggaa tgtcgctcga cgcggtcgaa gtcgacctct cgaaatcgtt
     1321 cgtggcgggc atgggctacg tcgcgctctc ccgcgtgcgc acgctcgcgg ggctccgcct
     1381 tctcgggatc aataatcagg cgctcatgat caacccggag gtgctcgagt tcgatcggga
     1441 gcttctcgaa gcctccaaaa agcaagagga gtggatcgcg ggcatcagtc ccgccgaaaa
     1501 gaaaaagctc cacgaagaat ttatcgtgcg gatcggcggc acccccggca agaaactcga
     1561 aaaaaaaacg aaagtcccga ccatcgaggt aacccgccaa tttcttctcg agaagctttc
     1621 gctcgagact attgcggcga aaagggaatt gaccgtggag acgatcgtca gccacatcga
     1681 gaagctccgc gaaaaggatc ccgcgctcga tatcgagcac atccgaccgc acaagatcat
     1741 cattgagaaa gtgaaagacg cgttcaagaa agcgggcgat acgcggctca cctcggcgaa
     1801 aaatattctg ggagagaaga taagttttct cgaaatacgc atcgcgaggc ttttcctcga
     1861 ttcgtagcgc tcactgctcg agcgccaggc gcctcggatg cgcgctcgtt ccggtaaata
     1921 cccatagccg cgaggcaatg aagctccatg agaacgtcgc gacggtcgag aggccggcgg
     1981 cgatgatgtc gttcaaatgc aggaggtcgg cgaagacgta catgcttatc gcgttcacga
     2041 cgagacttat tccggcgacg ctgtagaagc gcgccgcttc cgcaagggtg agcttgccct
     2101 tcatgccgaa cgtccatgaa tgattgaatt tcaagctggt cgccgtgccg aatccgaatg
     2161 aaacaatctt ggcgagaatg atgcgggccg cgaagaacgg aatgagccgc gtcatataga
     2221 cgtacgagaa gaagtcgacg gcggtgttga ggaggcctac cgatgcaaag cgcatggcct
     2281 gcgacgcggc gtcatttccg cctctttcat gatcgcccca gtagatttgc atcatttccc
     2341 tgaatgcgcg gaagacgacc gacggcttcg ctcccgaagc gcggccggca gcacgctccc
     2401 tgtgtccgac cggaacctcc gcaaacgata cgccgttgcg cttgaggcgc atgaggattt
     2461 cggcgctcgc catcgccccg ttcgaataaa cgggaatgga gagaagcgtt tcgcgtcgga
     2521 agagcttgaa tgcgcagtcg atgtcgcgca cgctgagatc gaagaggatc ctgttgagga
     2581 tattccatcc gcgggcatag attttccgga tgaacggatc ctttcgcgac tctctgaagc
     2641 ccaatacggc attacggctt tccgcgtagg gaaggagctt tttcagatcg cgcatgtcga
     2701 actgcatgtc cgcgtcgctc cagaagacga attccttttg ggcggcaaga atgcctgtct
     2761 tgagcgccgc gccgtagccg agattttcgg aatgatggat gacccgcacg cgggattccc
     2821 gcgccgcgat cgattcggct atttcccccg tgccatcgat tgatccgtcg tctacgatga
     2881 tgatctcgta atcatccttt cggaaaagcc cgtggagcac cgcttccgct tccatgaccg
     2941 cgtgttctat attcgaggct tcgttgcggg cagggaaaaa cacgctcaga ccgaagggat
     3001 atgcgcctgt gttcatactg ctatcctaga agccgaaaat taagaaatga tgagcttcat
     3061 ttcggcgccg cattgcctat tttttgagat cctcgtagct ttcgaaatcg tggatgaaaa
     3121 ggccgccgaa agagggcgaa gcgccaagcg ccgaaacgat attctcggcg tcgctctgaa
     3181 cgtccctctg ctttttgccg taaaacgtga tgcttgaggg agaggtctta ttcgcggcga
     3241 tgccgacgag tatgcgcacg ttcggcgcgt aattgtttgc gaaatttatc tcatcgcttg
     3301 agagctcaat cgcgccattg tgccccgatg acttgttcct atatgccatg acgaggatct
     3361 cgccgtgggc cgtctgcgag agcgcccgta tcgcctgaaa gaccgtggga gcggaagagc
     3421 ccgcataagg gacgttcggt atgtcgcgcg tcgccccgtc gaaccagaag ggaaccgtga
     3481 acccgagagt gagatcggga gccgcgctcc ggatgtgccg cgccgattcg tcgatcattt
     3541 cgacgaagtc ggacaggagc tcctgttgtc cggaatcgga atcgaaatcg tcgagagcct
     3601 gcggttcgat gtcgaatacc attccgccga gtttttggtc gctatcggcc gcggcgttgt
     3661 aggcggcgac ataatcgatg aggtattcga cgtcatcgag attcttcgga aggatccatt
     3721 cgggcgctcc gcccaggcct tcgacgcgga tgccgtactc ggaagcctcg ctgacgaaat
     3781 cgcggaggct cgcgtcgaat tcgtctttct tccgctcctc gttcctgtcc cttatcgact
     3841 tgaagtcgag cgcgtcgtcg atgcgaatgt agatatccgt aaagccggaa gctttggcgg
     3901 aagagaaata atcgtccagc ttctttttgg tatcgaagct gccgggagct ttccattcga
     3961 ttgccgccac cttcggcttc tggcccgccg cgcctggatt ccgctcttcg agggccgaaa
     4021 acgagagcgc tccgcccctg ctcgtttccg gatgcgccag aaagcggccc gcaagcgcca
     4081 ccgcggcaag gatgaggcca agagcgataa ggatgagaat ttttgtcctg gtatgtacgg
     4141 tttctttcat aagattaggc gctgaggaag atgatgaact tgaagatcgc gtatccgctg
     4201 agcgccagga taagcgtcgc ggtagtggca tattcgcgcg gcagattgcg gccagtgacg
     4261 aattggtaga attttttcct gactacgtaa tcatacttca ttttgaacca ccagaaatag
     4321 gcgatgaagc ccgctttcac cttgaggaga ttgtagccgg gaacgagcgg caggatccac
     4381 ggatggtcct tcctgcccat cctgctccat gccgccactt cgatcgagaa ataaaacgcg
     4441 tatagaagca ggatgttttc tgggtggaag atggagaaga atgcggcaag gatgcggatc
     4501 ggttcggtgc cgaagatgaa cagcttgtag gctcgttcgg tcttcaaaag aaaatgcgta
     4561 ttggaatgga atacgacgcg catattgagg atgaacagct cgttcacgga gcagttccat
     4621 ttcgtcgcgc gctgcttgaa gagctttgcg gcggtggccg gcgcttccgt ttcgaccagc
     4681 gtgtcgcaga aggtcactcc ctttttgtgg ctcccttcga gatggaggag gagcgtccgc
     4741 tgctggtcct cgcctccgta ttgtccggag tgcttatgag attgggcgat caggtccttt
     4801 gtatggaaaa ggccgatcgc tcccgacacg ttgccgatcg atccgctgtc ggacctctgg
     4861 cgtttgccga aatacatgct tttgcggtat tcgaaacgct gaagcttgtt gagaagtccg
     4921 gggcgcactg ccggcatgac gttgaaagcg accgcgctgt aatcttcgtc aagcaggttg
     4981 gtgggaatga cggtgtgtcc gatatgggta tcgtcgtcga ggatgagggt gtacggcgtt
     5041 tcgatatggc tggcgtagaa attgatgctc atcgatttgt tgaggctctc ctcattctcg
     5101 aatacccgaa cgccgaacga gcgggccacc tcggcggtgt cgtcggtcga agcatcggaa
     5161 atgacgatga tatgcttcgg cggcacgtgg acgagcgctt gcttgatcgt ttcgccgata
     5221 agcacttcgc cgttatgaca gacgatgagc acagttagct tttccggatc gcagaagagt
     5281 ttgccgtgct tttcctcgcg gcggaagagc gacaatacga agatgagtcc gtcagtcgcc
     5341 gcagtaagga aaaggatccg gtccgccgtc gaatcgaaga agacgtgcgg cgcgaacatg
     5401 ttcttgtaga agtaaatgac caagccggcg aaggcggcaa tgaccaggag ggaaaggacg
     5461 atatttttca cattctccaa tatgatgtgt gttcgtgcat ccataccatg cgcaatgcag
     5521 ctccatgtat ggagcctcag tgcatgcacg gcaattctat gtctcaaaca ttaaatttta
     5581 atgagccgga tttcggga
//
LOCUS       DASZYS010000042        11122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_50258, whole genome shotgun sequence.
ACCESSION   DASZYS010000042 DASZYS010000000
VERSION     DASZYS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50258"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHF05_02565"
     CDS             complement(<1..315)
                     /locus_tag="VHF05_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79836.1"
                     /translation="MHPFALLDLGQVTAYLLIVAISSAVCGIICYRLGRERDAGRALH
                     ISAIDLSHTYCLNCSKTESSTCVMFLTDRTTGVSKLYHYPNCSASSLPEKGINFRIEH
                     LHS"
     gene            complement(399..839)
                     /locus_tag="VHF05_02570"
     CDS             complement(399..839)
                     /locus_tag="VHF05_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79837.1"
                     /translation="MKVSWVFEIALLSTVIAVVACAEGFEAVADLSLIVNVVAISFCF
                     ATKASAASGHPAPISQIKTGRIYRIHYQHPVMARHGDEWIADPMQAVVLLEDVKKREY
                     RLYHYAQRTHGIQLWIGAAYEAIRTGNFAYPVELRFKDFPEFKG"
     gene            992..1489
                     /locus_tag="VHF05_02575"
     CDS             992..1489
                     /locus_tag="VHF05_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79838.1"
                     /translation="MEKLAVALGKHEEHAKALAEATEKIADLEQMREERRWINEVVDR
                     LKASVKENVTAHIMPLVVRGGYNSPYVDYTGPYVEVSFEDKFGARQPVLQIGFDFCET
                     GAEIWVWEHPDDCSKRCATFPYADQQAIEYVCDYAKSLDLWRISADDLKNDQGVHLPG
                     GMVVE"
     gene            complement(1557..2900)
                     /gene="aspS"
                     /locus_tag="VHF05_02580"
     CDS             complement(1557..2900)
                     /gene="aspS"
                     /locus_tag="VHF05_02580"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HVS79839.1"
                     /translation="MERILIKDLEQHKGEEVRIQGWVAVRRDQGKMIFLDFRDRTGLV
                     QGVVLPNSPAMETAKELRQEFVVAVGGAVNERPEKNKVAGKQNGSIELEIKSIEVLSA
                     AETLPFDIESELNLDTYLDYLPLTLRRDRSRAIFKVQSEIAKAYRSFLDGKGFTEFQS
                     PKLIGDDAEGGANVFEVPYFYGKKAHLGTSPQFYKQIMVGVFERVYSIGNVFRAEKHS
                     TTRHLNEYTSMDAEMGFIKDHKDLMNLEADFVRHLVAHLKETCAAEFALHGAEFPKVP
                     SEVPAMKLREAQELIFKETGKDKRNEPDLEPEDERFLCEWAAREKESDFIFITHYPVS
                     KRPMYTFEDPEDPGFTRSFDLLFRGVEITTGGQRRHDYNSLIEGIRMKGLDPEKFSFY
                     LQAFKYGMPPHGGWGMGLERLTQKMLGLANVKEATLFPRDINRIDTRLSEAAVEE"
     gene            complement(3350..4321)
                     /gene="trpS"
                     /locus_tag="VHF05_02585"
     CDS             complement(3350..4321)
                     /gene="trpS"
                     /locus_tag="VHF05_02585"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVS79840.1"
                     /translation="MSNGKKILLSGVKPTGRPHIGNYFGAMKQFVELQDEYACHFMIA
                     DYHALNFIQNGPQLKELIMDLALDYLAIGLDPEKSVIFKQSDVTAHTELTWIFDTITT
                     MAYLQRAHAFKDAEAKDKEISVGTFNYPLLMACDILLYDADVVPVGQDQKQHVEYARD
                     TAEKFNRIFGQGENDAAFKLPNELILENVAVVPGTDGRKMSKSYGNTIPLFATDEEIK
                     KAVMSIVTDSSGERPENVYAIHKLFKSEAELAPLYEENKGKYKNLKEALIADLKAFIT
                     PLREKREILSQDRVHVLDILRNGGEKAKEIADKKMELVRERIGVKLY"
     gene            complement(5027..5722)
                     /locus_tag="VHF05_02590"
     CDS             complement(5027..5722)
                     /locus_tag="VHF05_02590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79841.1"
                     /translation="MAQYDAWEREYRNSKLLTKDADPQKDTLRFIKFLKKDAGIRPER
                     VRVLDIGSGTGRNGNHFASLGADVVGLEISDTAVRIARERAGKENVRAEYFKHDIGSP
                     YPLDDESFDIAIDVTSSNSLLGSERDVYLREAHRVLKSDPDSGYFFVKTLCKDSDENA
                     KALLKKSPGPEIDTYRMPELGLVERVWSRKDFEEFYGQLFEIIHLEKKESYTRLNNRS
                     YKRMFWIAYMQKK"
     gene            complement(5732..7348)
                     /locus_tag="VHF05_02595"
     CDS             complement(5732..7348)
                     /locus_tag="VHF05_02595"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVS79842.1"
                     /translation="MMDGINLLLISVTIFSAFLGFTLIFHGRGSRISTAYAANVVAIL
                     CWMASMFFYRNSLGDSDVIFWVIILYISATLIASSFLFFTHFFPESEPRLNIAARSLL
                     IFLPNVVLIFVIALTPFVIQSAHANRPHEPIIIFGQYYALYVVYILGYFSYGFYRLIA
                     KFVKSDDQTRRNQIKYLLLGYMVATSIAFVSNLFLPWFGVFTLNWLGQVSGIIMVIFA
                     TYAIFRYKLFNVRVATTEFLTFSLWAFILIRAIFSETVNDQNGNLALFFVSLIFGIFL
                     IRSVIKEVRQREEIQKLAENLERANAQLREVDQMKSEFISLATHQIRSPLTAIKGYSS
                     LILEGDYGEAPKQIRDVVKIIFDSCQNLVVIVNEFLDISRIEQGRMKYDKSDFEMLDL
                     VKETIEELLPNVEQAKLGIEFHHGRDETYHVYADRNKVKQVVSNLIDNAIKYTPQGGI
                     TVKLGKRIGKVVLAIEDTGVGIDPAEKDKLFAMFTRAKDANKINVTGTGLGLYVARQM
                     MQAQGGEIWVESPGKGKGSTFFVELLESGG"
     gene            complement(7468..8589)
                     /locus_tag="VHF05_02600"
     CDS             complement(7468..8589)
                     /locus_tag="VHF05_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79843.1"
                     /translation="MSKPLDKVGFLVHSRDLRDFKRKFPILKYFPERALLFLTKLAPP
                     IVVSKITGLVSAEGSPVEGFVLGIPMTAKQMMEDRAAALRKIKQAVRLAERKGVGIIG
                     LGALTASFSRGGLDVAGTTKVGVTTGRAYTVKTVTEYVLHAVGLFGIDLGNVRIAIVG
                     AAGAIGSGCAKILAKHGVRNFLLIDLERKAEHLKRHMEDITGRHLGARIEISHRIGDI
                     KGWDIVIAATNAPEVLIKPDDLLPGAIVVNDAQPSDISPEVLDMDDTLVIEGGVIRTP
                     GISCNFNMGLARRNDNFCCLGEALVLAHQGRFADFALGELDMKLIEEITAKAKTLNMT
                     ISEIQNARGIVPEEKIARIREAIRLREAERIAAKISVSP"
     gene            complement(8570..9337)
                     /locus_tag="VHF05_02605"
     CDS             complement(8570..9337)
                     /locus_tag="VHF05_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVS79844.1"
                     /translation="MKTSHVTAFFQLIFYALCLPLMKFFCRLEVAGRENLKALPPAFI
                     IAANHAAEIDSPFVRAALPFFSRYAPVYFVSNTRDRFHGYGWRSLFYGGRIFALLGGY
                     PVYKGHHDYAYALQNHLRLLREGAVVSIFPEGKISRSGALGEAHGGVAFLAKETDSPV
                     LPVAISGLYGMTFADFFLRRRRAFVRIGAALLPNEIVPEAAPAVDDYKRGAEAVMRSI
                     GGLLAEEKRSAIPVPLTPLKVVEPANSTMHIHEQTPR"
     gene            complement(9636..10706)
                     /gene="dnaJ"
                     /locus_tag="VHF05_02610"
     CDS             complement(9636..10706)
                     /gene="dnaJ"
                     /locus_tag="VHF05_02610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVS79845.1"
                     /translation="MANKDYYEILGVSKSATKDDIKKAFRKLAHKYHPDKEGGDESRF
                     KEVNEAYSVLGDDKKRAEYDAYGRTFSGSNGNGGAGFDPNGFGFDFSGFANGQDFDLN
                     DIFGNIFGGGGQRVKRGRDISIDIELTFKEAIFGATRSVLISKASQCDRCKGNGAEPG
                     TEKVTCTTCNGKGRIREMRRSIFGSFATETTCDTCYGSGKMPKVKCKECGGLGVVKKQ
                     QEVKIVIPAGIDNGEMIRLSGQGEAVPGGVSGDLYVKIHIRRDPTFVKDGNDIRMDLK
                     VKLSDALLGGEYSVATLDGDIKVKIPAGVAHGEVLRVKGKGVPIEKNKRGDLLIRVGI
                     SLPGKLSGEAKRLVEALRKEGI"
BASE COUNT         2515 a         2973 c         2972 g         2662 t
ORIGIN      
        1 cgagtgcagg tgctcgatgc gaaagtttat gcccttttcc ggaagtgaac tggccgagca
       61 gttcggataa tgatagagct ttgaaactcc cgtggtgcgg tcagtaagaa acatgacgca
      121 cgtggagctt tcagtcttgg aacaattgag acaatacgta tggcttagat cgatggccga
      181 aatatgaagc gcgcggcccg catcgcgttc tcggcccaat cgatagcaaa tgattccgca
      241 gacggctgat gagatggcga caatcaatag atatgccgtc acctgcccga gatcaaggag
      301 cgcaaagggg tgcatgggaa acctcctctt taatcatcga accgttttcc ccggttgtca
      361 atgcgcgtaa aaggccgcgg aacatccgcg gccatttctc atcctttaaa ttctgggaaa
      421 tccttgaacc tcaactcaac aggataagca aagtttcccg ttcgaatcgc ctcgtatgca
      481 gcgcctatcc agagttggat gccgtgcgtt ctttgcgcgt agtgatagag cctatactca
      541 cgctttttta cgtcttcaag caagaccact gcctgcatgg gatcggcgat ccattcatcg
      601 ccatgccttg ccatgacagg atgctgataa tgaatgcggt aaatccttcc tgtcttgatc
      661 tggcttatgg gcgcaggatg ccccgaagcg gccgaggcct tcgttgcaaa acagaacgaa
      721 atcgccacga cattcacgat aaggctgaga tctgccactg cctcaaaccc ttccgcacat
      781 gcgacgacgg caatgactgt gctgaggagc gcgatctcga atacccaact gactttcatt
      841 tcttttcaaa cctttccgtc gtataaaata acacacgctt gcaatttgtc aatttagatg
      901 ctagaatcaa agcaggagga agtacaaaga gatgtatttt tggattttta tcgcagtgtt
      961 ggttcttgga ttcgtggcca tatatgaatc cttggaaaaa ctggccgttg cattgggcaa
     1021 gcacgaggag catgcaaagg cgctcgcgga agcaacggaa aaaatcgcag accttgagca
     1081 gatgagggaa gaaagacggt ggattaacga agttgttgac cggcttaaag cgagcgtaaa
     1141 ggaaaacgtc acggctcaca ttatgcctct ggttgtacga gggggttata actctcccta
     1201 cgtcgattac actggaccgt atgtagaagt gtcctttgaa gataaattcg gtgcgcggca
     1261 gcctgttctg cagattggtt tcgatttctg cgaaaccggt gctgagatat gggtctggga
     1321 acatcccgat gattgctcaa aaagatgcgc aactttcccc tacgcagatc agcaggccat
     1381 tgaatacgtc tgcgattatg caaaatcact cgatctctgg cgtatcagcg cagacgacct
     1441 gaaaaatgat caaggcgttc atctccccgg cggcatggtc gtggaataaa cgcaaaaaaa
     1501 gcaaagaccc ggaatatgtt ccgggtctct ttttattcct aaaatttctt taaaaactat
     1561 tcctccaccg ccgcttcaga gagcctcgtg tcgatgcggt tgatgtcgcg cgggaagagc
     1621 gtcgcttctt tcacgttcgc gaggccgagc attttttggg tgaggcgctc gagtcccatt
     1681 ccccacccgc cgtgcggcgg catgccgtac ttgaaggcct ggagatagaa cgagaatttc
     1741 tcgggatcaa ggcctttcat gcggattccc tcgataaggg aattgtaatc gtgccgacgc
     1801 tggccgccgg tcgtgatctc cacgccgcgg aagaggaggt cgaagcttcg cgtgaagccg
     1861 ggatcttccg ggtcctcgaa ggtatacatc gggcgcttcg agacgggata atgcgtaata
     1921 aaaataaagt cgctctcttt ctcgcgcgcc gcccattcgc agaggaagcg ctcgtcctcg
     1981 ggctcgaggt cgggctcgtt gcgcttgtcc tttcccgtct ccttgaaaat gagctcctgc
     2041 gcttcgcgaa gcttcatagc cggaacttcg ctggggacct tggggaactc ggcgccgtga
     2101 agcgcgaatt ccgcagcgca ggtttccttg agatgcgcga cgagatggcg cacgaaatca
     2161 gcctcaagat tcatgagatc cttgtggtcc ttgatgaagc ccatctcggc atccatggaa
     2221 gtgtattcgt tcaaatggcg agtcgtggaa tgcttctccg cgcggaagac gttgccgatc
     2281 gaatacacgc gttcgaagac gccgaccatg atctgcttgt agaactgcgg cgacgtgccg
     2341 aggtgcgcct tcttgccgta gaagtacgga acctcgaaga cgttcgcgcc gccctcggca
     2401 tcatcgccga taagcttcgg cgactgaaat tcggtgaagc ctttgccatc gaggaaggaa
     2461 cgataggctt tggcgatttc cgactgcact ttgaagatgg cacgggaacg gtcgcggcgg
     2521 agcgtgagcg gcaggtagtc gagataggta tcgagattga gctcactctc gatgtcgaac
     2581 ggaagcgttt cggcggcgct cagaacctcg atgctcttga tctcaagctc gatcgagccg
     2641 ttctgcttgc cggctacctt gttcttctcc ggacgctcgt tgaccgcgcc gccgacagcg
     2701 acgacgaatt cctgcctgag ctccttggca gtttccatcg cgggagaatt cggcagtacg
     2761 acgccctgca cgagccccgt cctgtcgcgg aagtcgagaa agatcatctt gccctgatcg
     2821 cggcgtacgg caacccagcc ttgaatccgc acttcctctc ccttgtgctg ttccaaatcc
     2881 ttgataagta ttctctccat tgtttttgta aattaaatcg cccttacaaa ttcgcgaaaa
     2941 ttcgcgagct tgtaagggcg ttttataacg cggtgccacc ttacttctcc cccaccactt
     3001 ttggcatcat cctcgatgtg tgcttcgcgc aatgccaaaa gtggtggggg acttcgttta
     3061 ttcatctgag gctcggccgg tgtcacattc ggcgtcagtg cctgtacaca atttataata
     3121 cattaaaaac cttatttttc aagcggaagt tggaccttca aatttgaatg aaatgcaagg
     3181 cacgagcctc aggtgagctg agacagccgg aagcagcgca agaccttttg agataatttc
     3241 tgaattatcc aaaaggtgta ctgatcctgt aaggatcgaa cgagccgtac cgagtatacg
     3301 gtgaggagca catttggcga aacaaagcag ttcgccaaat ttgagggttc tagtataatt
     3361 tcactccgat tctttcccgt actaattcca tcttcttgtc cgcaatctct tttgctttct
     3421 ctcccccatt gcggagaatg tcgagaacat gcacgcgatc ttgcgagagt atttctctct
     3481 tctcgcgaag cggcgtgatg aacgccttga gatcggcgat gagagcttct ttcagattct
     3541 tatacttgcc cttgttttct tcgtagagcg gggcgagctc ggcttcggac ttgaagagct
     3601 tgtggatcgc atagacattt tccggcctct cgcccgacga gtcggtcacg atagacatca
     3661 ctgccttctt gatttcttcg tcggtcgcga aaagcgggat cgtattgccg tagctcttcg
     3721 acatcttcct gccgtcggta cccggaacta cggcaacatt ctccaaaatg agctcgttcg
     3781 gcaatttgaa cgccgcatca ttttctcctt ggccaaaaat gcgattaaac ttctcggcag
     3841 tatcgcgcgc gtattccaca tgctgtttct ggtcctgtcc taccggcaca acatccgcgt
     3901 cgtaaagtag aatgtcgcat gccataagaa gcgggtagtt gaacgtgccc acgctgatct
     3961 ccttgtcctt cgcttccgca tccttgaacg catgcgcacg ctggagataa gccatcgtcg
     4021 tgatcgtatc aaagatccag gtgagctcgg tgtgggcggt tacgtccgac tgcttgaaga
     4081 tgaccgactt ctcggggtcg agaccgatag cgaggtagtc aagcgcgaga tccatgatga
     4141 gctccttgag ctgcggcccg ttctgaatga aattgagcgc gtgataatcc gcgatcataa
     4201 agtggcacgc gtattcatcc tgcagctcaa cgaactgctt catggcaccg aagtaatttc
     4261 cgatatgcgg cctccccgtc ggcttcacgc cggagagcag aattttcttc ccattggaca
     4321 tcccgttgtg agatttcagg tcgttcgcga agacgcgaac tgtcccatat atctcgtctt
     4381 taatgaagag tttttgttta tgaaataaat cccgaatcca tccagcttcg ccacctgaaa
     4441 ttctacaacg ggatcatagt caaaataata gcagaaaact gtcattttgc atgattttga
     4501 ggataattga catttttaaa tttatatact acaaagaggt caggagacgg catatgcccc
     4561 aaccagcaaa ggaatatctg agacaaaagt ggacaagctc agagagcggc agcacacgag
     4621 ggataacatt ttgtagccta catgatgctt gtaatacgaa ggatgcatta ctaaacgctt
     4681 atcaacagct caaaatcgag cattatgttg aagatatagt tcggcttcgc gagtttgctg
     4741 atagggatta caaaaatggt gaataccgtc aatctgtaac cttaaaagca atggcaacag
     4801 agctcgaaat tacagctgca acgtacatca atttatgccg cggatagcga caatgcagag
     4861 gtccggtgcg aatcaaaacg cgccgggctt tttcttttgc gctggttccc gctcatggtc
     4921 tatcccccta tgcgcgcgtg acatgcctat attctgcaga atataggcat gatgttggga
     4981 cttataattt tttcgatctt tccatcgacc gttgcctatc tactctctat ttcttctgca
     5041 tgtacgctat ccaaaacatg cgcttatagc tcctgttgtt gagccttgtg tacgactcct
     5101 ttttctcgag atggatgatc tcgaataatt gcccgtaaaa ttcctcgaaa tctttcctgc
     5161 tccagacacg ctcgacgagg ccgagctcgg gcattctgta ggtatcaatc tcaggcccgg
     5221 gcgatttttt gaggagggcc ttagcgtttt cgtcgctgtc cttgcataat gtttttacga
     5281 aaaaatatcc cgaatccgga tctgacttga gcactcgatg cgcttcgcga agatacacgt
     5341 cgcgctcgct gccgagaagg gagttcgagc tcgtgacgtc aatcgcgata tcgaagcttt
     5401 cgtcgtcgag cgggtacggg ctgccgatgt catgcttgaa atactcggcg cgtacgttct
     5461 ccttccctgc cctctcgcgg gcgatcctta ctgcagtatc ggaaatctcc aatccgacaa
     5521 cgtctgcgcc gagcgacgca aagtggttgc cgttcctccc cgtccccgag ccgatatcga
     5581 gaacgcgaac tctctccggc cggatgccgg catctttttt gagaaacttg atgaaccgga
     5641 gcgtgtcctt ctgcggatcg gcatccttgg tgaggagttt ggagttcctg tattccctct
     5701 cccatgcgtc gtactgagcc atgcgacaat cttatcctcc cgactcgaga agttcaacga
     5761 agaaggtcga tcccttgccc ttgccgggcg attcgaccca gatctcgcct ccctgtgcct
     5821 gcatcatttg gcgggccaca tagagcccaa ggcccgttcc ggtaacattg atcttgttgg
     5881 catccttagc gcgcgtgaac attgcaaaaa gcttgtcctt ttcggccgga tcgatgccga
     5941 cacccgtatc ttctattgcg aggaccacct tgccgatcct tttgccgagc ttcacggtaa
     6001 tgccgccctg cggcgtgtat ttgatcgcat tgtcgatgag gttcgaaaca acctgcttca
     6061 ccttgttgcg atcggcatag acatgatagg tctcgtcccg gccgtgatgg aactcgatgc
     6121 cgagcttcgc ctgctcgacg ttcggcagga gctcctcgat cgtttccttc acgaggtcga
     6181 gcatctcgaa gtcgctcttg tcgtacttca tcctaccctg ctcgatgcgg gagatgtcga
     6241 ggaattcgtt tacgatgacg acaagatttt ggcaggaatc gaagatgatc ttcaccacgt
     6301 ccctgatctg cttcggcgct tcgccgtagt cgccttcgag aatgagcgaa gaatagccct
     6361 tgatagccgt aagcggcgag cggatctggt gcgtcgcaag cgaaatgaat tcggatttca
     6421 tctgatcgac ttcgcgaagc tgagcgttcg cccgttcgag attttcggcg agcttctgaa
     6481 tttcctcgcg ttgccgcact tccttgatga cgctcctgat gagaaagatg ccgaaaatga
     6541 gcgacacgaa gaagagagcg agattgccat tctggtcgtt caccgtttcg gagaagatgg
     6601 cgcggatcag gataaatgcc cagagcgaga acgtgaggaa ttcggtcgtc gcaacgcgca
     6661 cattgaaaag cttgtagcgg aaaattgcgt aggttgcgaa gatcaccatg atgatgcccg
     6721 agacctgccc aagccagtta agggtgaaga ccccgaacca cggcaggaag aggttggaca
     6781 cgaaggcgat cgatgtggcg accatgtaac caagcaggag gtatttgatc tggttgcgcc
     6841 gcgtctggtc gtccgatttt acgaatttcg cgatgaggcg gtagaagccg taggagaagt
     6901 agccgaggat atacacgacg tagagcgcgt aatactgccc gaagatgatt atcggctcat
     6961 gcgggcggtt ggcatgcgca ctttggatga cgaacggggt gagggcgata acgaatatca
     7021 gaacaacgtt cggtaggaag atgaggagcg atcgcgcggc aatgttcagc ctcggctcgc
     7081 tttcgggaaa gaagtgagtg aagaaaagga agctcgaggc gatgagcgtc gcggaaatgt
     7141 agaggatgat cacccagaaa atgacatcgg agtcgccgag cgaattgcgg taaaagaaca
     7201 tcgacgccat ccagcacagg atggccacga cgttcgccgc gtaggcggtg ctgatgcggc
     7261 ttccacggcc gtggaaaatg agcgtgaagc ccaggaaggc gctgaagatc gtcaccgata
     7321 tgagaagcag attgatgccg tccatcatta tgtgcgaaac ggcgcacgga ggctccgcgc
     7381 gctgcgcgcg aacaagccgc cgagacttcg gccgttatag cctaattgta gtatgaaaaa
     7441 gaaaaagcgc cgacggatcg ccgcgcatta tggggatacg gaaatctttg cggcaatccg
     7501 ctccgcttcg cgcagacgga tcgcttcccg tatgcgcgcg attttttcct ccggcacgat
     7561 ccctcgcgca ttctgaattt ctgaaatggt catgttgagc gtttttgcct tggcagtgat
     7621 ctcttcgatg agcttcatat cgagttctcc gagcgcgaaa tccgcgaaac ggccctggtg
     7681 agcaagcacg agcgcctcgc cgaggcagca gaaattgtcg ttgcggcgcg caaggcccat
     7741 gttgaagttg cacgagatgc ccggcgtgcg gatgacgccg ccttcgatca cgagcgtatc
     7801 gtccatgtcg agcacttcgg gggaaatgtc cgatggctgg gcgtcgttta cgacaatcgc
     7861 gccgggcagg agatcgtccg gcttgatgag cacttcggga gcgttcgtcg ccgcgatcac
     7921 gatatcccat cccttgatat cgccgatccg gtgcgagatt tcgatgcgcg cgcccagatg
     7981 gcggccggtg atgtcttcca tatgccgctt cagatgctcc gccttgcgct cgagatcgat
     8041 gaggaggaaa ttgcgtacgc catgttttgc caagatcttg gcacagcccg acccgatcgc
     8101 gcccgccgcg ccgacgatcg cgatgcggac gttgccgaga tctatgccga aaagcccgac
     8161 tgcatggaga acatactccg tgacggtttt caccgtgtac gcccgaccgg tcgtcacgcc
     8221 gactttcgtg gtgcccgcga catcgaggcc gccgcgcgag aagctcgccg tcaaagcccc
     8281 gaggccgatg atgccgaccc ccttcctttc cgcgagccgc accgcctgct tgatctttcg
     8341 aagcgccgcc gcgcggtcct ccatcatctg cttcgcggtc atgggaatcc ccaggacgaa
     8401 tccttctact ggcgagccct ctgcagaaac caggccggta atcttcgaca cgacgatcgg
     8461 cggagcgagc ttcgtgagaa agaggagcgc tcgttcggga aagtatttca aaatcgggaa
     8521 ctttcgcttg aagtcccgaa ggtcgcgcga gtgcacgaga aagccgactt tatcgagggg
     8581 tttgctcatg aatgtgcata gtgctgttcg ccggttcaac gacttttaac ggagtgagcg
     8641 gcacggggat tgctgaccgc ttctcctcgg caaggagccc gccgatgctc ctcatcactg
     8701 cttcggcgcc tcttttgtag tcatcgaccg cgggcgccgc ttcaggcacg atctcattgg
     8761 gaagaagcgc cgcgccgatg cgcacgaacg cgcggcgccg gcgcaggaag aaatcggcaa
     8821 acgtcatccc atagagtccc gaaattgcga ccggcaggac gggagaatcc gtttccttcg
     8881 cgaggaacgc gacgccgccg tgcgcctcgc cgagcgcgcc gctgcgtgaa atcttccctt
     8941 cggggaaaat gctcacgacg gcgccttcgc gaagaaggcg gagatggttc tgaagcgcgt
     9001 acgcgtagtc atggtggcct ttgtacaccg gatagccgcc aagaagggcg aaaatcctgc
     9061 ctccataaaa aagcgaccgc catccatatc catggaagcg gtccctcgta ttggaaacga
     9121 aatataccgg cgcgtagcgg gagaagaacg gcaatgccgc gcgcacgaaa ggagaatcga
     9181 tctcagcggc atgattcgcc gcgatgatga atgcgggcgg aagcgctttc aaattctcac
     9241 gaccggcgac ttcgagcctg cagaaaaatt tcatgagcgg caggcacagg gcgtagaaga
     9301 taagctgaaa aaatgcggtt acgtgagaag ttttcatcag aacctatccc aaaaaataat
     9361 ggcttggaga gaaggcgaaa tttcgagcga aaaaacagaa aaccgagcac ggcgtagcga
     9421 aagctacgac acgcgaggtt atctggtttt gcagccgaaa tttcagtttt tctccagcca
     9481 agataggcat tatgttatat cggttatttt tgggatagat tctagaccgt cgcgaatgca
     9541 tttcggagct tcaactgccg aaacttttaa gcacaactat tataccacgc atggttcatc
     9601 atgaattgct ggactgctgt ggagatcttt actggctata ttccctcctt gcggagcgcc
     9661 tcgacgagcc gcttcgcctc gcctgaaagc ttgcccggaa gagaaatgcc gacgcggata
     9721 agaaggtcgc cgcgcttatt cttctcaatc ggcactccct tgcctttgac acgcagaact
     9781 tcgccatggg caacgcctgc tggaatcttc accttgatgt ctccgtcgag ggttgctacc
     9841 gagtattcgc cgccgagaag cgcgtcggaa agcttcactt tgaggtccat ccggatatca
     9901 ttgccgtctt tcacgaacgt cggatccctg cgaatgtgga ttttcacata gaggtcgcca
     9961 gagacgccgc cgggaaccgc ttcgccttgg ccggaaaggc gaatcatctc gccattgtcg
    10021 atgcccgcag gaatgacaat tttcacttcc tgctgcttct tcacaacgcc caaacctccg
    10081 cattccttgc acttgacctt cggcatcttg ccggagccgt agcacgtatc gcacgttgtc
    10141 tccgtcgcga acgatccgaa gatggagcgc cgcatttcgc ggatgcggcc ctttccgttg
    10201 caagtggtgc atgtcacctt ttcggttccc ggctcggcgc cgttaccctt gcatctgtcg
    10261 cactgcgagg cttttgagat gaggacgctc cgggttgcgc cgaaaatggc ttccttaaag
    10321 gtaagctcga tgtcgatcga gatgtcgcgt cctcgcttca cccgctggcc tccgccaccg
    10381 aaaatatttc cgaaaatgtc attgaggtcg aaatcctgcc cgttggcgaa tcccgagaaa
    10441 tcgaagccga agccgttggg gtcgaagccc gctccgccgt tgccgttcga gcccgagaac
    10501 gtcctgccgt atgcgtcgta ttcggcgcgc ttcttgtcat ccccaagcac gctgtatgcc
    10561 tcgttaacct ccttgaatcg agattcgtcg cccccctcct tgtcggggtg gtacttatgg
    10621 gcaagcttcc tgaacgcctt cttgatgtca tctttggttg cgctcttgga aactcccaat
    10681 atctcgtagt aatctttgtt tgccatgtta tttcgattta gttcttgctt gccccgtagc
    10741 aatttgtcat caaatttgct gcggggtttg ccattgttcg ttatgattag gatttgcggg
    10801 aatcctaggc tttacggcca gcaggaagat gaggaaagcc gtttataggg ccgtggattc
    10861 ggacggattg cttattgcca caaaattata tacctttcat tccccctctt caaaccctct
    10921 ccgagaggcc ccgcattccc cttgcccgcc cacattgatg aaccccagtc aatgctttct
    10981 gaaagaaggg cagttcccgc gcgtctgtat gggcagagga gacccgttaa ttgcccaaca
    11041 gtttatcgtc ccgtgagatc gaaaagcttc ttgatcgatt tgaagaggaa ttgcgagctt
    11101 tgcccgcccc caccattgca cc
//
LOCUS       DASZYS010000043        13073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_110017, whole genome shotgun sequence.
ACCESSION   DASZYS010000043 DASZYS010000000
VERSION     DASZYS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110017"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHF05_02615"
     CDS             <1..299
                     /locus_tag="VHF05_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family peptidase"
                     /protein_id="HVS79846.1"
                     /translation="TSTTLRPSSSATIYLSPSGGFSNSVSYTLSGAPSGAGYVFNPSS
                     VSGPTYGQSRLTLYVPDNTPISTYSMTVTGRSSDGQTHTTNVILDVAAANGGQQ"
     gene            365..910
                     /locus_tag="VHF05_02620"
     CDS             365..910
                     /locus_tag="VHF05_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HVS79847.1"
                     /translation="MQNQTVKTAHLRQQRTDKGVRRVSAGFMLVYTVLILSIMLIVGG
                     IFVNTVVKEVGMSRDESESLKAFYAADSGVECVRYFKNKYDAFNTQVPQSTYDCGIGS
                     SFTAGGNPPTSQCTEKEYTFQVGGFTSGACMNITVDMTPRTITVNGNPVIVCDTNVIA
                     DGMNTCNLSSATVERSRWETL"
     gene            complement(1006..1833)
                     /locus_tag="VHF05_02625"
     CDS             complement(1006..1833)
                     /locus_tag="VHF05_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HVS79848.1"
                     /translation="MELGVLLSFVFGTIIGSFLNVVAARYNTGLSINGRSMCMTCGRK
                     LRAMELVPVLSYAALRGRCRTCGSAISIQYPLVEFVTGVLFAGVYAVTIGASATVGPV
                     AIISAAFAAAVWSILTVITVYDIKHKIIPDSLVFIFIGLSFLHMLVHYGSLLWGEPYL
                     VSALLAGPIVAAPFLVLWLVSRGTWIGLGDAKLALGIGWFLGLSGAISAILLGFWSGA
                     FLSVCLLLMNKLPERRWLKRLGVRHRALSGKTEIPFAPFLILGIAIVYFFHLSVIAF"
     gene            complement(2055..2555)
                     /locus_tag="VHF05_02630"
     CDS             complement(2055..2555)
                     /locus_tag="VHF05_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVS79849.1"
                     /translation="MKKHHREFSKGFTQAPAAGAGFTLIELLVVIAIIGILSAVVLAS
                     LNTARAKGADAAIKSDLQSVRDEAEVYYDGTGGNTYGAAMTAAVCPGTGTMFSDDTSI
                     ADAIANAVTAAGKTDTVCATDGSNSYFVIVPLKTSGYWCIDSSGQSKGEDGNGNGTGY
                     SGYACL"
     gene            complement(2691..3191)
                     /locus_tag="VHF05_02635"
     CDS             complement(2691..3191)
                     /locus_tag="VHF05_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVS79850.1"
                     /translation="MKNKSYIPTKGFTQAPAAGAGFTLIELLVVIAIIGILSAVVLAS
                     LNSARAKGADAAVKSDLSSIRAQAELYYDTNNNYAGATFARRTCDTVTGTNMFVSDPI
                     VKNAIDNARGAGGGPTECWSDGQHYIISAKLKSNNDQWCIDSNGSAREISTANWTGSA
                     NGCPAS"
     gene            complement(3302..3751)
                     /locus_tag="VHF05_02640"
     CDS             complement(3302..3751)
                     /locus_tag="VHF05_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVS79851.1"
                     /translation="MMKNMRSRGFTLIELLVVIAIIGILSAVVLASLNSARAKGADAA
                     VKSDLSSIRAEAEIYYDNNNKYGASATSCTTAGTMFADDNQIKNALAQAKLTGQGGQV
                     CTISSTGSDYLVATQIKSTTHYWCIDSTGVARDVATVSSGAITCPAS"
     gene            complement(3881..5092)
                     /locus_tag="VHF05_02645"
     CDS             complement(3881..5092)
                     /locus_tag="VHF05_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVS79852.1"
                     /translation="MIFNYKAIDKNGETKEGVIDAISMDVAISSLQKRDLIISSIVPQ
                     DESQSFLQHIPFLNRVSNKEVVILSRQLATLFEAQVSALRIFKLMSSEVENPQLRKSL
                     TEIADDLQGGSSISKALAKHPNVFSPFYINLVKAGEESGRLNETFNYLADYLDRTYEV
                     TSKAKNALIYPAFVIVTFIGVMILMFTVIIPKISSILTDSGQALPIYTQIVLDVSSFF
                     VHYGVFLLVAVVIGALALWRFAMTPEGQLSLSSFKLSVPYVGDLYKKLYLSRIADNLH
                     TMIVSGIPMLRSLEITANVVGNDIYKQILEQSLTAVKGGSSLSSSLSQYPEIPGILVQ
                     MVKVGEETGQLGKILKTLASFYQREVMNAVDTLVGLIEPVMIVVLGLGVGILLASVLM
                     PIYNVASTFGS"
     gene            complement(5187..6257)
                     /locus_tag="VHF05_02650"
     CDS             complement(5187..6257)
                     /locus_tag="VHF05_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVS79853.1"
                     /translation="MSTLTPQAELSELVDSVVKENGSDIHLSEGRQPVLRVSSFLIPL
                     VKKPMYSREDVEGILKIMLTPDNEKILETKKEVDFAYTHNSKYRFRGNAYNHLGKLAV
                     ALRLIPQETRSFAELNLPPVLEMFAEKKQGFFLVVGPTGQGKSTTLATMIEMINTKRL
                     ERIITIEDPIEYIFTPKKSIIDQREVRVDTPSFATALNGTFRQDVDVIMIGEMREPET
                     IATAVTAAETGHLVYSTLHTNDAAQSVDRIIDSFPPEQQNQIRLQLASSLVGILSQRL
                     VPRVSGGLIPVCELLINTNAVANLIRENRTHEIPMMIETGMQEGMIDLNRSLVDLVRA
                     GEITVEEAFSHSSNPKTLERML"
     gene            complement(6267..6656)
                     /locus_tag="VHF05_02655"
     CDS             complement(6267..6656)
                     /locus_tag="VHF05_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVS79854.1"
                     /translation="MQKGNYKMLLVDDDQFLLNMYSLKFGKNGYDVTAISNPQEALTK
                     LREGLSPDVIVLDIIMPGIDGLQVLQTIRDEKLASNAAVVMLTNQGDGIERAKKLGVA
                     GYIVKATTIPSEVVKEVSDIMAHQQSK"
     gene            complement(6680..8419)
                     /locus_tag="VHF05_02660"
     CDS             complement(6680..8419)
                     /locus_tag="VHF05_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVS79855.1"
                     /translation="MSILDILATKGVITPDDAKAFKEEAETSNESIEKVLSKHGVTNQ
                     AIVESKGEYLDIPVRTIDTNGIKGQILHYIPEESARHYKIVPIGLADDVLEVGIVDPD
                     NIEARDALNFISAKINIPFKIFLITEQDFDKVIALYKGLSEEVNEALSELETQNSKFD
                     KELSLDDAESGGDAISAKDGKIDTKIIEDAPVTKIVATILRYATDGTASDIHVEPMRE
                     TIRVRFRVDGILNTSLVLPIKVHSAVVARIKILSNMRLDEKRKPQDGRFSAKIEGRKI
                     DFRVSTFPTYFGEKIVMRILDHDKGVRPLDQIGISDRNIATIRKALQRPYGLILISGP
                     TGSGKTTTLYSMLGEIDRDKLNVLSLEDPIEYNIEGVSQSQVRPDIGYTFANGLRTTL
                     RQDPDVIMVGEIRDKVTARLAIQAALTGHLVFSTIHTNSAIGVIPRLVDMGVDPYLIA
                     PTLVLAIAQRLVSALCPGAGKPMPIEGSLKIMLDKQFEDLPDEFQSLVPKVSEVYEAS
                     PTDECPKGTHGRMAVMEVLEMDRELEAVILKDPTELAIGKLARSKGMLSMKEDAIIKA
                     MNKEIPFEEVNGL"
     gene            complement(8480..8929)
                     /locus_tag="VHF05_02665"
     CDS             complement(8480..8929)
                     /locus_tag="VHF05_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79856.1"
                     /translation="MGKNSKTIIAILLVFGVMFYAYVSFFNNSGTPTDETASEDASST
                     DASGDVGEQLVTLLDQLKTIRMDGSIFTSSQFLALRDETAAVVNQPIGRNNPFAPVGT
                     DGAFISNFTFGNDSQVENSLGSQPVDQGSSTAASSSATTTGSSQTGF"
     gene            complement(8931..9473)
                     /locus_tag="VHF05_02670"
     CDS             complement(8931..9473)
                     /locus_tag="VHF05_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79857.1"
                     /translation="MNSNVTPFFLLALAIALFYVYINPEYSKIDALRAQEAQYSDALD
                     RVQEISQIRDQLLTKYNSFSPDDLNKLQTMLPDTVDNVHLVLDMANVAGNYDLSLSNI
                     KVDRPQQDLGSSDETGQVPQYQTVNVAFDTSGTLANYTNFLKDIEASLRITDVTGISI
                     RPLDSGKYQFTTVVNTYWLK"
     gene            complement(9478..10086)
                     /locus_tag="VHF05_02675"
     CDS             complement(9478..10086)
                     /locus_tag="VHF05_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79858.1"
                     /translation="MDTKFQTSFIPKRPIVASNEGPIKTKHPLNFISLVCTIVFVVAV
                     LGAGGMFAYEHYLDGKIASMDGQLAAAKSSIQSDIINQFIRLDTRLNVANQLLRNHVA
                     TSVLFSMLQNQTVKTVQFKDLAYTSNGDAINVVLHGQAGSYNAMALQSAIFSQDKFIH
                     SPTFSEMNLDSSGNVLFTLNASVDKDYLAQRNNLDQLTANNF"
     gene            complement(10435..11598)
                     /gene="pilM"
                     /locus_tag="VHF05_02680"
     CDS             complement(10435..11598)
                     /gene="pilM"
                     /locus_tag="VHF05_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HVS79859.1"
                     /translation="MSSLYDILSSKFTTMGLLDSFFKKKESSVLGIDIGSSAIKVVQL
                     KKQGNKAILETYGELALGPYAGVQVGQATNLPTDKVIEALTDLLKEKEVNITTRNCGI
                     AIPFRSSLMTLIEMPTTDRKQLEQMIPIEARKYIPVPITEVALDWTVIPRDEYEFNDA
                     VDEEGVKPVAATNKSDVLVIAIHNDIISNYQSIIQKAMVNATFFEVEIFSTMRAVLDQ
                     NSTPTMILDMGASSTKLYIVEKGIVRTSHTINKGAQDVTATLSKSMEIPVQKAEILKR
                     EQGLIGSYEDRPLKGIMSVTLDYIFSEANRVLLSYERKTAKNIAKIILVGGGAALKGL
                     AAVAQESFDTEVVAGDPFSKVQTPAFLEEVLKSTGPEFAVAVGIAIRKLQEIS"
     gene            11605..12258
                     /locus_tag="VHF05_02685"
     CDS             11605..12258
                     /locus_tag="VHF05_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79860.1"
                     /translation="MKLLQTAWETVLDALFPKRERIAALEKLSVDELLNLLPPASLPP
                     GGDGIFPLFDYHNERVRTIVWEIKYRHNRRLIGRMAELLHQEMLESASDFASMGGLPP
                     LLVPIPTSAARRRERGGSQTELLAEAIAKMEIGSIAEYAPNALMKVRETESQTALSRA
                     KRLRNLVGTFEADKVAVSGRDILLLDDVVTTGATIAEAKRALREAGAGEILSFAISH"
     gene            complement(12302..12916)
                     /locus_tag="VHF05_02690"
     CDS             complement(12302..12916)
                     /locus_tag="VHF05_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79861.1"
                     /translation="MKTQRMNVVLLPSEEDRQKFGRFADELAAKFNLMMIVNNKEYIP
                     HLTLYQINVKEHHLERAREVTRILSKQSSFFETVEDPGRGDNNSFDYNVHLGRKIFLL
                     HVNALRVFQEVRAHDQAIDIPDADSTDAQRENIKLYGVKNVLKLYRPHVTIGCVTDED
                     DLELILHFLEKKPVNPVHFTELALAKINAFGQVYEIVENCPLAD"
BASE COUNT         2828 a         3494 c         3750 g         3001 t
ORIGIN      
        1 gcacgtcgac aacgcttcgc ccgtcatctt cggcgaccat ttacctttcg ccttccggcg
       61 gcttcagcaa ctccgtgtcc tataccctct cgggcgcgcc ttcgggagcc ggatacgtct
      121 tcaacccatc gagcgtttcg ggcccgacct acggccaaag ccggctcacc ctctacgtcc
      181 cggacaatac gccgataagc acctacagca tgaccgttac cggccggtca tcggacggcc
      241 agacgcacac aaccaacgtc atcctcgatg tggcggctgc aaatggaggc cagcaataac
      301 atgagatact cgcgcaatgg caaaaaataa acggcggatt catccagcac taagccaacc
      361 tgttatgcaa aatcaaacgg taaaaacagc gcatttgagg cagcagagaa cagacaaggg
      421 ggtccgcaga gttagtgctg gattcatgct cgtctacacg gtgctcatct tgagcatcat
      481 gctcatcgtg ggcggaatct tcgtcaacac cgtcgtcaaa gaagtgggaa tgtcgcgcga
      541 tgaatccgaa tcgctcaagg ccttctatgc cgctgattca ggcgtcgagt gcgtccgcta
      601 cttcaagaat aagtacgacg cattcaacac gcaggttccc caatcgacct acgactgcgg
      661 catcggatcg agctttaccg cgggtggcaa tccccctacc agccaatgca ctgaaaagga
      721 atacaccttc caggtcggcg gttttaccag cggcgcatgc atgaacatca ccgtcgacat
      781 gacgccgcgc accattaccg tcaacggcaa cccggtcatc gtctgcgata cgaacgtgat
      841 cgccgacggc atgaacacct gcaatctctc aagcgccact gtggagcgat caagatggga
      901 gacattataa ggggtgactc gaatgtaatt tgaaccgtaa aaaccgccct gtaagatagc
      961 gcttcgcgct atcttacagg gcggttttcc gtacgctcac aacatctaaa acgcgatcac
     1021 gctcaagtga aagaagtaga cgatcgcgat gccgagaatg aggaacggcg cgaacgggat
     1081 ttccgtcttc ccgctgaggg ccctatggcg aacgccgagg cgcttgagcc atcgcctctc
     1141 gggaagcttg ttcatgagaa gaaggcagac gcttaggaac gcgccgctcc agaaaccgag
     1201 aaggatcgcc gagatcgcgc ccgaaagccc gaggaaccag ccgatgccga gcgcgagttt
     1261 cgcatctccg agcccgatcc acgtaccgcg cgagacaagc cagaggacaa ggaatggcgc
     1321 ggcgacgatg gggccggcaa gaagcgccga gacgaggtac ggttcgcccc acaggaggct
     1381 cccgtagtgc acaagcatgt ggagaaacga aaggccgatg aagataaaaa cgagcgagtc
     1441 gggtatgatc ttgtgcttga tatcgtagac tgtgatgacg gtaaggatgc tccataccgc
     1501 cgcggcaaat gccgcagaaa tgatggccac aggccctacc gtggcgctcg cgccgatagt
     1561 cacggcatat acgcccgcga agagcacgcc tgtcacgaat tcgaccagcg gatattggat
     1621 cgagatcgcc gaaccgcacg tccggcagcg gccgcggaga gccgcgtagc tcaacaccgg
     1681 cacgagttcc atggcgcgga gtttcctgcc gcaggtcatg cacatcgacc tgccgttgat
     1741 tgacaagcca gtattgtagc gggcggcaac gacgttcaaa aagcttccga tgatcgtacc
     1801 gaacacgaat gacagcagta ctcctagttc catcccgtta gagataggaa attacaaata
     1861 tgaatttgca atgcctatat gaatttaaac cagtaaaaag cttttccgac ccgttttcaa
     1921 tttccgcgat ctctaacggg atccattttt cccaaggatt ataacggaat tataacctgg
     1981 ccttccatat cgccaaaatc tgaataaaaa aacaaccgcc ctctttgggg cggctgcttt
     2041 tcttagattt tagattagag gcaggcgtag ccgctgtagc cggttccgtt tccgtttcca
     2101 tcctcgccct tcgactgtcc cgagctgtcg atgcaccagt agcccgaagt cttcaaggga
     2161 acgatcacaa agtacgaatt cgatccgtcc gtcgcgcaga ccgtatcagt cttgcccgcc
     2221 gcagtaacgg cattggcaat agcatctgca atcgaggtgt catcggagaa catcgttcca
     2281 gtgcccggac agacggcagc tgtcattgcc gcgccgtaag tattgcctcc cgttccgtca
     2341 taataaactt ccgcttcgtc gcgaacgctc tgaaggtccg acttgatggc cgcatcggcg
     2401 cccttggccc gagcggtatt caagctcgca agcacgactg ccgagaggat gccgataatc
     2461 gcgataacga cgagaagctc gataagcgtg aacccagcac ctgctgctgg tgcttgggtg
     2521 aagcctttgg aaaattccct gtgatgtttt ttcataattc gattatttgt taaatatttc
     2581 agacaatcgg cgcttttctc agcattgctg cggaaggcct gatttcatga atttattata
     2641 catgaactca accaaagctt ttgaaataaa agtatttcct gtggatggcc ttacgacgcg
     2701 ggacagccgt tcgccgaacc ggtccagttc gcagtgctaa tctctcttgc actgccatta
     2761 ctgtcgatac accattggtc attattagac ttgagcttgg cggagattat ataatgctga
     2821 ccgtccgacc agcattcagt aggccctccg ccggcccccc gggcattatc aattgcgttc
     2881 ttgacgatcg ggtcactcac aaacatattg gtaccagtta cagtatcgca tgttctcctg
     2941 gcaaaagttg caccagcata attattattg gtatcgtaat acaactctgc ctgagcgcga
     3001 atgctcgaaa ggtcggattt caccgccgca tcagcgccct ttgcacgagc gctgttgagg
     3061 cttgcgagaa cgactgccga caagataccg atgatcgcga tgacgacgag aagctcgatg
     3121 agcgtgaagc cagcaccagc tgctggtgct tgggtgaagc ctttcgttgg gatatagctc
     3181 ttgtttttca ttttttaatt ataatacgtt caattaggta tttcaggtca tttaatccac
     3241 atgcaaaaaa ccatcgggat cccggtggtt ctttgctgct aagtgcagaa gtcgtatagg
     3301 cttatgaagc cggacaggta attgcacctg aagaaactgt tgcaacatct ctagcgactc
     3361 ctgtactgtc tatgcaccaa taatgggtag tcgatttgat ttgtgtcgca accaagtagt
     3421 ctgaaccagt gctgctaatc gtgcagacct gaccgccttg accggtcaat ttcgcctgcg
     3481 cgagagcgtt cttgatctgg ttatcatcgg caaacatagt gcctgcagtg gtgcatgagg
     3541 tcgcagaagc gccgtattta ttattattgt cgtaataaat ctccgcttca gcacgaatgc
     3601 tcgaaaggtc ggatttcacc gccgcatcag cgccctttgc acgagcgctg ttgaggcttg
     3661 cgagaacgac tgccgacaag ataccgatga tcgcgatgac gacgagaagc tcgatgagcg
     3721 tgaagccgcg cgaccgcata ttcttcatca taatgttaaa acctaataat taattcctag
     3781 taaaccacgc cgtgtccgcc cggggacacg aaaagctcca aatattatac cattcggaaa
     3841 ttgcgcttcc ccgccccaat ggggataacc tcaacagagc ctatgagccg aaggtcgagg
     3901 cgacgttgta gatcggcatg aggacagatg cgaggagaat gcctacgcca aggcctaaaa
     3961 cgacgatcat gaccggctcg ataaggccca cgagcgtatc cacggcgttc atgacctcgc
     4021 gctgatagaa gctggcgagg gttttgagga tcttgccgag ctgtccggtc tcctcgccca
     4081 ccttgaccat ctggacgaga atgcccggaa tctccggata ttgggacaac gacgaagaca
     4141 gggagctgcc gcccttgacc gccgtgaggc tctgctcgag gatctgcttg taaatatcgt
     4201 tgccgaccac gtttgccgtg atctcgagcg agcggagcat cggaatgccg gaaacgatca
     4261 tggtatgcag gttatcggcg atgcgggaaa ggtagagctt cttgtagaga tcgcccacat
     4321 acggcaccga gagtttgaag ctcgagagcg agagctggcc ttcgggagtc attgcgaagc
     4381 gccagagggc gagggcgcca atcaccaccg ccacaaggag gaacacgccg tagtggacga
     4441 agaagctcga gacatcgagc acgatctgag tatagatagg gagcgcctgg ccggaatcgg
     4501 tgagaatcga gctgatcttc ggaatgatca cagtgaacat gaggatcatg acgccgataa
     4561 aggtgacgat gacgaaagcc ggatagataa gggcgttttt cgccttcgaa gtgacttcgt
     4621 atgtcctgtc gaggtaatcg gcgagataat tgaaggtttc gttcaggcgg cctgattcct
     4681 cgcccgcttt cacgagattg atatagaaag gagagaagac gttcgggtgc ttggcgagcg
     4741 ctttcgagat ggaacttccg ccctgaaggt cgtcggcgat ctccgtgagg ctcttgcgga
     4801 gctggggatt ctccacttcc gacgacatga gcttgaaaat gcggagggcc gagacttgcg
     4861 cctcgaagag tgtcgcgagc tggcgggata ggatgacgac ttccttgttg gaaactcggt
     4921 tgaggaacgg aatatgctgc aggaacgact ggctctcgtc ctgcggaacg atggaggaaa
     4981 tgatgaggtc ccgcttctga aggctcgaga tggcgacatc catgctgatg gcgtcgatca
     5041 cgccctcttt cgtttcgccg ttcttgtcga tcgccttgta attgaagatc atggaatcaa
     5101 tttttaattt tcaatgaatt ttcaatttct taatatctca gtccttgaaa attgatcatt
     5161 gtgtcttgat tgtaaattga aattttctac agcatcctct ccagcgtctt gggattgctc
     5221 gagtgcgaga aggcctcctc taccgtgatt tcccccgccc tcaccaggtc gacgagggag
     5281 cggttgaggt cgatcatgcc ctcctgcatg cccgtctcga tcatcatggg gatttcgtgg
     5341 gtacggttct cgcggataag gttcgccacg gcgttggtat tgatgagaag ctcgcagacc
     5401 gggatgaggc cgccggaaac gcgcggcacc aggcgctggg aaaggatgcc gaccaaagac
     5461 gacgcgagct ggaggcggat ctggttctgc tgctccggcg ggaacgagtc gatgatgcgg
     5521 tcgacggact gcgccgcgtc gttggtatga agcgtcgagt agacgaggtg gccggtttct
     5581 gcggccgtca cggccgtcgc gatcgtctcg ggctcgcgca tttctccgat catgatgacg
     5641 tcgacgtcct ggcggaaggt cccgttgagc gccgtggcga aggacggcgt gtcgacgcgc
     5701 acctcgcgct ggtcaatgat cgacttcttc ggcgtgaaga tgtactcgat cgggtcctcg
     5761 atggtgatga tgcgctcgag ccgcttggta ttgatcatct cgatcattgt ggcgagcgtc
     5821 gtcgactttc cctgccccgt cgggcctacg acgagaaaga agccctgctt cttctcggcg
     5881 aacatttcga gcaccggcgg gaggttgagc tcggcgaacg atctcgtttc ctgcgggatg
     5941 aggcgaagcg cgacggcaag cttgccgaga tggttgtagg cgttgccgcg gaaacggtat
     6001 ttcgaattgt gcgtgtacgc gaagtcgact tccttcttgg tttcgaggat cttttcgttg
     6061 tccggcgtca gcatgatctt caaaataccc tccacgtcct cgcggctgta catgggcttt
     6121 ttgacgagcg ggatgaggaa gctcgatacg cgcaagaccg gctgcctgcc ctcggagagg
     6181 tgaatgtccg agccgttttc cttgacgacc gagtcgacaa gctcgctgag ttccgcctgt
     6241 ggagtgagtg tgctcattgc ttttgtttat ttactttgct gatgcgccat gatatcgctc
     6301 acctctttca cgacttcgga aggaatggtc gtcgccttga cgatgtagcc ggcgacgccg
     6361 agcttcttcg cccgctcgat gccgtcgccc tggttggtga gcatgacgac cgccgcattc
     6421 gaagcgagtt tctcgtcgcg gatcgtctga aggacctgaa ggccgtcgat tccgggcata
     6481 atgatgtcga gaacgatcac gtcgggagag agcccttcgc gaagcttcgt cagcgcttcc
     6541 tgcggattcg aaatggccgt cacgtcgtag ccgttcttgc cgaacttaag cgaatacatg
     6601 ttgagcagga actggtcgtc gtcgaccaga agcatcttgt aattcccttt ctgcatattg
     6661 aggattatac cctacttttt tataaaccat taacctcctc gaacgggatt tccttgttca
     6721 tcgccttgat gatcgcgtcc tccttcatgc tgagcattcc cttcgaacgg gcaagcttgc
     6781 cgatggcgag ctccgtcggg tccttgagga tcaccgcttc gagttcgcgg tccatctcga
     6841 gcacttccat gaccgccatg cggccgtggg tgcccttcgg gcactcgtcc gtgggcgatg
     6901 cctcgtagac ctcgctcacc ttggggacga gcgattggaa ctcgtcggga agatcctcga
     6961 actgcttgtc gagcatgatt ttgaggctcc cttcgatggg catcggcttc ccagctcccg
     7021 ggcagagcgc cgagacgagg cgctgagcga tggcgaggac gagggtcggc gcgatgaggt
     7081 acgggtcgac gcccatgtcg acgaggcgcg gaatgacgcc gatggcgctg ttggtgtgga
     7141 tggtcgaaaa cacgagatgg ccggtaagcg cggcctggat ggcgaggcgc gccgtcacct
     7201 tgtcgcggat ctcgccgacc atgatcacgt ccgggtcctg gcggagcgtc gtgcggaggc
     7261 cgttcgcgaa cgtatagccg atatcggggc gcacctgcga ctggctcacg ccctcgatgt
     7321 tgtattcgat cggatcttcg agagaaagca cgttaagctt gtcgcggtcg atctccccga
     7381 gcatggaata gagagtcgtc gtctttccgg atccggtggg gccggaaatg agaatgaggc
     7441 cataggggcg ctggagcgcc ttgcggatgg tcgcgatatt gcggtcggaa atgccgatct
     7501 gatcgagcgg ccggacgccc ttgtcgtgat cgagaatgcg catcacgatc ttctcgccga
     7561 aatacgtcgg gaaggtcgag acgcggaagt cgatcttgcg cccttcgatc ttcgcggaaa
     7621 agcggccgtc ctgcggcttg cgcttctcgt cgaggcgcat gtttgagagg atcttgatgc
     7681 gggcgacgac cgccgagtgg accttgatcg gaagcacgag gctcgtattt aaaatgccgt
     7741 cgacgcggaa gcgcacgcgg atcgtctcgc gcatcggctc gacatggata tcggaggcgg
     7801 tgccgtcggt ggcgtagcgg aggatggttg cgacgatctt cgtgaccggg gcgtcctcga
     7861 tgatcttggt gtcgatcttg ccgtccttgg ccgaaatggc gtcgccgccg ctctccgcgt
     7921 catcgagcga gagctccttg tcgaatttgg aattctgggt ctcgagctcg gacagcgcct
     7981 cgttcacctc ttccgagagg cccttgtaaa gagcgatcac cttgtcgaaa tcctgttcgg
     8041 taatgaggaa gatcttgaag ggaatattga tcttcgccga aatgaagttc agggcgtcgc
     8101 gcgcctcgat gttgtccgga tcgacgatgc cgacctcgag cacgtcgtcg gcaaggccga
     8161 tcggcacgat cttgtagtgc cgtgcggatt cctcgggaat gtaatggaga atctgcccct
     8221 tgatcccgtt cgtatcgatc gtccgcaccg gaatgtcgag atactccccc ttgctctcga
     8281 cgatcgcctg attggtcacg ccatgcttcg agagcacctt ttcgatgctc tcattgcttg
     8341 tctcggcctc ttccttgaag gccttggcgt cgtcgggagt gatgactccc ttggtggcta
     8401 aaatgtcgag gatactcatg gcttcaaggg cgcgagcccg cttggaaaat gggcttaatt
     8461 gaggcgccgt tcttccggtt taaaatcccg tctggctgga accggtagtc gtcgcggagg
     8521 acgaggcggc ggtcgagctt ccctggtcaa ccggctgcga accgagggaa ttctccacct
     8581 gcgaatcgtt gccgaacgtg aagttcgaaa tgaaggcgcc gtcggtgccc acgggggcga
     8641 acggattgtt cctgccgatc ggctgattca cgacggccgc cgtctcgtcg cgcaatgcga
     8701 ggaactgcga cgacgtgaag atcgaaccgt ccatccgaat ggtcttcaat tggtcgagca
     8761 gggtaacaag ctgctccccc acgtcgccgg aagcatcggt ggagctcgcg tcttccgacg
     8821 ccgtctcgtc ggtcggcgtg ccgctgttgt tgaagaacga aacataggcg taaaacatga
     8881 cgccgaacac caggaggatc gctatgattg ttttggaatt ctttcccata ttacttgagc
     8941 caataggtgt taacgaccgt cgtgaattgg tatttgccgc tatccaaagg ccggatgctg
     9001 atgcccgtca cgtcggtaat gcgcaggctc gcctcgatat ccttgaggaa attcgtgtag
     9061 ttcgcgaggg ttccggaggt atcgaaggcg acgttcaccg tctgatattg gggcacctgg
     9121 cccgtttcgt cgctcgagcc gaggtcctgc tggggccggt cgaccttgat attggaaagg
     9181 gagaggtcgt aattgccggc gacgttcgcc atgtcgagca cgaggtggac gttgtcgacg
     9241 gtatccggaa gcatcgtctg gagcttgttc aggtcatccg gcgagaagct gttgtacttg
     9301 gtgagaagct ggtcgcgaat ttgggaaatc tcctgcacgc ggtcgagcgc gtcggagtat
     9361 tgcgcttcct gcgctcggag cgcgtcgatc ttcgagtact cggggttgat gtacacgtaa
     9421 aacaaggcga tggcgagcgc gaggaggaaa aatggtgtga cgttgctgtt catcgcatta
     9481 gaaattgttc gctgttaatt ggtccagatt gttgcgctgg gcgagataat ccttgtcgac
     9541 cgacgcgttc agggtaaaga ggacgttgcc ggaagaatcg aggttcattt cggagaatgt
     9601 gggcgaatgg atgaatttgt cctggctgaa gattgcggac tggagcgcca tggcgttgta
     9661 gcttcccgcc tggccgtgca ggacgacgtt gatggcgtcg ccgttcgagg tataggcgag
     9721 atccttgaac tggacggtct tgaccgtctg gttctggagc atcgagaaga ggacggacgt
     9781 cgccacatga ttgcggagaa gctggttggc cacattaagc ctcgtgtcga gcctgatgaa
     9841 ctggttgatg atgtcgctct ggatggagga tttcgcggca gcaagctggc catccatcga
     9901 ggcgattttg ccgtcgagat aatgttcgta tgcgaacatg ccgccggcac ccagcactgc
     9961 gacgacgaac acgatcgtgc agacaagcga aatgaagttc agaggatgct tcgttttgat
    10021 cggaccctcg tttgatgcga cgatcggacg cttgggtatg aacgatgtct ggaacttggt
    10081 gtccatcccg ttagaagcag atcccgttag aaatctcaca ttcggtcgaa tgctcgtttc
    10141 cagctttgct ctgcttgaat tctgttagct tgtaattcat ggttcgtaga tttctaaacg
    10201 ggacagatca cgctcacggc gcagtctgtc ggattagcct gatttctttt tgtaaacatc
    10261 tcgaccatgg cagataagat atcgtgatcg cggcctctaa cgggatccat aaaatggcgc
    10321 gcgcaccgac tgcgcagaag cgcgtccggc gcacatgata gcttgatgaa aataattata
    10381 catgcaccgg tgtgcatgca caattaaact ttccacatgg agcgcggcga gcgcttatga
    10441 aatctcctga agcttcctga tggcgatgcc gaccgccacc gcgaactccg gaccggtcga
    10501 tttgaggacc tcctcgagaa acgcgggcgt ctgcaccttg gagaacgggt cgccggcgac
    10561 aacttccgta tcgaagctct cttgcgccac cgccgcgagg cccttcaagg ccgcgccgcc
    10621 gcctacaagg atgatcttgg cgatgttttt cgcggtcttg cgctcatatg aaagaagcac
    10681 cctgtttgct tcggagaaaa tatagtcaag cgtcaccgac atgatgcctt taagcgggcg
    10741 gtcttcgtag gagccaatga gcccctgttc gcgcttcagg atttccgcct tctgcaccgg
    10801 aatctccatc gatttggaaa gtgtcgcggt gacgtcctgg gcgcccttgt tgatggtgtg
    10861 cgacgtcctc acgatcccct tttcgacgat gtagagcttc gtggaagatg cccccatgtc
    10921 gaggatcatg gtcggggttg aattctggtc gagcacggcg cgcatggtgc tgaagatctc
    10981 gacttcgaag aaggtcgcgt tcaccatcgc cttctggatg atcgactggt agttcgagat
    11041 gatatcgttg tggatcgcga tgacgagcac gtcggacttg ttcgtcgcgg cgaccggctt
    11101 cacgccctcc tcgtcgacgg cgtcgttgaa ctcgtattcg tcgcggggaa tgaccgtcca
    11161 gtcgagcgcc acttcggtga tcgggacggg aatatatttc ctggcctcga tcggaatcat
    11221 ctgctcgagc tgcttgcggt cggtcgtcgg catctcgatg agcgtcatga ggctcgagcg
    11281 gaagggtatg gcgatgccgc aattgcgcgt agtgatgttg acttcctttt ccttgagaag
    11341 atcggtgaga gcttcgatca ccttgtcggt cggcaggttc gtcgcctggc caacctgtac
    11401 gccggcatac ggcccgagcg ccagctcgcc gtacgtctcg agaatcgcct tgttgccctg
    11461 ttttttgagc tgaacgactt tgatcgccga agatccgatg tcgatgccaa gaacgctcga
    11521 ttcttttttc ttgaagaagc tgtcgaggag acccatggtc gtgaattttg aagatagtat
    11581 atcatataaa gaactcattc ccccatgaaa ctcctgcaaa cagcatggga aacggttctc
    11641 gacgcgctat ttcccaaacg cgaaaggatc gcggcgctcg aaaagctttc cgtcgacgag
    11701 cttttgaatc tcctgccgcc cgcgtcgctt ccgcccggag gcgacggcat ctttcccctc
    11761 ttcgattatc ataacgagcg cgtccgcacg atcgtatggg agataaaata ccggcacaat
    11821 cgccggctca tcgggcggat ggcggagctc ttgcatcagg aaatgctcga atccgcttcg
    11881 gacttcgcct cgatgggcgg cttgccgccg ctcctcgtac ctatcccgac ctccgccgca
    11941 agacggcggg agcgcggcgg gagccagacc gagcttctcg cggaagcaat cgcgaaaatg
    12001 gagatcggga gcattgcgga atatgctccg aacgccctca tgaaagtgcg ggagaccgaa
    12061 tcgcagacgg cgctttcccg cgcgaaacgc ctcaggaatc tcgtcggcac gtttgaagcg
    12121 gataaagtgg ccgtttccgg ccgcgatatc ctgcttctcg acgacgtcgt aacgaccggc
    12181 gccacgatcg ccgaagcgaa acgcgcgctt cgcgaggcag gggcgggaga aatcctctca
    12241 tttgcaattt cgcattaaaa aaatcccgtg agacggcgcg aagcctatct cacggggcaa
    12301 ctcagtctgc aagcggacaa ttctcgacaa tttcgtagac ttggccgaac gcatttatct
    12361 tcgcaagggc cagttcggta aagtgcacgg ggtttacagg tttcttctca agaaaatgaa
    12421 gtatcagctc gagatcgtcc tcatccgtca cacagccgat cgtcacgtgc ggacgataca
    12481 gcttcaggac attttttacg ccgtataact ttatattctc cctttgggcg tccgtcgagt
    12541 cggcatctgg tatgtcgatc gcctgatcgt gcgctcgcac ctcctgaaaa acgcggaggg
    12601 cgttaacatg gagcagaaag attttccggc cgagatgtac gttgtaatcg aaagaattgt
    12661 tatcgccccg tccagggtct tccaccgttt cgaaaaaaga agattgcttc gaaaggatcc
    12721 gtgtcacctc gcgagctctt tccaaatgat gttctttgac attgatctga tagagcgtca
    12781 ggtgaggaat atactcctta ttattgacga tcatcatgag attgaacttc gcggcaagct
    12841 catccgcaaa acggccgaat ttttgcctgt cttcttccga tggcagcaaa accacgttca
    12901 tacgctgcgt tttcatgcgt tgcatacgtt tccagcctcc ggaattacaa tgcggaattt
    12961 ggtatttttt gtcaatgaat taggagagtg tgggattcgg ctatccgtat tttcctgctg
    13021 gaaaatcgga cagcccgccc tcgcaccgtc gcgctccggg cttcgaatcc cac
//
LOCUS       DASZYS010000044        14825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_52531, whole genome shotgun sequence.
ACCESSION   DASZYS010000044 DASZYS010000000
VERSION     DASZYS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52531"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..895
                     /locus_tag="VHF05_02695"
     CDS             143..895
                     /locus_tag="VHF05_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HVS79862.1"
                     /translation="MLEEQYKIKTQVFEGPLELLLDLIEKRKLFINDISLSQVTDDFL
                     SYIKEKENFPLGLSANFILVASTLLLIKSKSLLPSIELTEEEQGSIDDLERRLKELER
                     IRELSLHVKSLFGKRIIFGRLQSRERKPVFAPDPAMTMANIVIAAQSVLNSLPKKEMK
                     PEVTVKKIISLEETIDKLTARIAKSLKLSFRDFAGHGTDSALNHEKKVEVIVSFLAML
                     ELAKQGIIAVSQNDLFDDIHMETESIGVPKYF"
     gene            900..1481
                     /locus_tag="VHF05_02700"
     CDS             900..1481
                     /locus_tag="VHF05_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVS79863.1"
                     /translation="MSGHTSALSARIEALLFYKAEPVSYKKLADLLSVSREELLDGLN
                     ELEKALEGRGIQLVRNDEEAFLGTSAAASELIEAIQREELSRDLGKAGLETLAIVLYK
                     GPVTRREIDYVRGVNSAFILRNLLVRGLVERVEGEGRGYSYRPTTDLLSFMGIGSRSD
                     LPEFAAVRAEMEKFAASDSEGSEGKEEKSDDAA"
     gene            1519..2541
                     /locus_tag="VHF05_02705"
     CDS             1519..2541
                     /locus_tag="VHF05_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79864.1"
                     /translation="MSFLTALQKQKFLSLTLLVGVLMTGYMFLRHIDPNIHAPSLSRN
                     TAAAEASLPATTTPPNAFKNVQIAAKAAIVLDARSGEVLYEKNADTALPLASLTKIMS
                     AITAADLASMNSTVTVDQFALETDGDNGLYAQESWKLKNLLDFSLIVSSNDGITAIAS
                     SLGGTILGETASAAAADPASAEAAFVGRMNNEAEKLDLPTLRFINPTGLDEGVKTGGM
                     GSARDVAKLLSYGISDYPSVFEATRYQSIDVTSENGITHTAVNTDAVLNSIPGLIASK
                     TGYTDLAGGNLAVAFDAGLNRPVVVVVLGSSYDGRFGDMATLVGATEDYIAGSTDKES
                     LASNKE"
     gene            2544..3623
                     /locus_tag="VHF05_02710"
     CDS             2544..3623
                     /locus_tag="VHF05_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79865.1"
                     /translation="MIIDIVKVFIPPVVAFILGVVLTPILTNYLYKNEMWKKKAGKKT
                     FDGQETPIFNKLHEGREVGTPKMGGIIIWFSAAVTIIGIWALSAIFPTENTLKLGFLS
                     RSQTWIPFGTLLLGALVGLIDDYLDIKGTGKHVAGGLPLKQRLSIVAIIGLLCGGWFY
                     FKLAVSSIGLPMNLVLNIGWLIIPLFAIVTMAIYSGGVIDGIDGLAGGIFASIFAAYA
                     GIAFYQQQIDLAAFCLCVVGAILAFLWFNIPPARYYMSETGSMALTVTLAVVAFMTDS
                     LAGGYGLLVLPVIALPLVVTAASDIIQITSKKFRGKKVFLVAPIHHHFEAVGWPSYKV
                     TMRFWVIGVVSAILGVILALLGVGK"
     gene            3740..4285
                     /locus_tag="VHF05_02715"
     CDS             3740..4285
                     /locus_tag="VHF05_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79866.1"
                     /translation="MGCWLSSAHAYRITYADARYTLTDSHIKQEFHAASGKGEWLSGN
                     EYPIQKNVTALVPPFGLYCRYRIIEGHPLSCLVGDAETENRLFAVVETYDYDKEGNAK
                     NPHTILVTLEETDTYPLRVKMRFDHQTRQLTADPNCQIEKFKAWIKLLNQDLNWSRHS
                     RKVHDSKMPQSVPNYAQAKAV"
     gene            4376..4636
                     /locus_tag="VHF05_02720"
     CDS             4376..4636
                     /locus_tag="VHF05_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79867.1"
                     /translation="MQREELPRMHSMSFGMKNGKGYVTYCGKMYPVENQDDCRFGSVF
                     GLGMHIEVCVLGHNNDLPGEKITDSKGRKVTLCRIERPIVAA"
     gene            4899..6014
                     /locus_tag="VHF05_02725"
     CDS             4899..6014
                     /locus_tag="VHF05_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HVS79868.1"
                     /translation="MKRAGKIDKPFLIAVIILVVAGYFIFSSASLGLLASQQAKYSNV
                     AFNQTFFGLFLGSIACIITSRLDYRIWRKYSLILFVLSAIMTLLVFVPHIGFSHGGAT
                     RWINLGVTTFQPSELLKISFIFYFSAWIAKYKDKVNSFKYGFLPFLVLIGIVGAILIK
                     QPDTGTFAVTFAAGLAIYLVAGASWLYLLLLVAGSALGLFVIAKLRPYVMARFTTFLD
                     PSADALGAGYQLQQSLIAIGSGRFLGRGFGQSIQKFNYLPEPIGDSIFAVMSEEFGFL
                     GSVTLVLLFLFLTVRGLKIAATSPDTFGRLVTVGIVILIISQAFINMGGMLAIMPLKG
                     ITLPLVSQGGTALFMTLAEIGIIVNISKFSKESKESA"
     gene            6146..7273
                     /locus_tag="VHF05_02730"
     CDS             6146..7273
                     /locus_tag="VHF05_02730"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HVS79869.1"
                     /translation="MKILFTGGGTGGHFYPIVAVAEEIKKQVVENKLVEAELYFMSPT
                     PYNAGILYERGIRFIPVAAGKQRTYFSILNFFDLFKTGWGIVTALWKVFNLYPDVIFS
                     KGGYTSFPVLVAARILRIPVVIHESDSVPGKVNQWSGKFAARIAVSFPDAAAYFPKDR
                     VALTGNPVRTEIALRSTEGAREFFSLEPNIPVILVLGGSQGSKIINDSMTDALPELLK
                     KYQVLHQSGKNNFVEAKETADVVLLNDDNKPRYHLVDYFNTVALRHAAGSADLVISRA
                     GSTIFEIAAWGLPSIIIPIRESRNDHQAKNAYAYARAGACQVVEEMNLTSNILAAEVS
                     RILDNVELRGKMAEAAKAFFRPRAAEAIALEILKIAAQHGG"
     gene            7279..8745
                     /gene="gltX"
                     /locus_tag="VHF05_02735"
     CDS             7279..8745
                     /gene="gltX"
                     /locus_tag="VHF05_02735"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVS79870.1"
                     /translation="MDTNNRNIVTRFAPSPTGFMHIGGVRTALFAYLWARKNRGTFIL
                     RIEDTDKEREVAGSIEHIQESLRWLGLEWDFGPDKPGTFGSCIQSERLPTYLEFAHRL
                     IEKGLAYPDPYSSEQVAEFRKQAEAEKRPFLFRNHRPEAFEKWDSTKPLRFKVPEIKR
                     YRWHDAVRGDLEAGEEALDDFILIKSDGYPTYNFAHIIDDFSMGVTHIMRADEFISST
                     PRFLSLYEALGIAPPVFVTLPPILRDDRTKKLGKRDGAKDILDYRAEGYLPEAMANFL
                     ALTGWNPGTEQEIFTMDDLIAAFDIAKIQKAGAAFNEEKLLWANREHIKNLSHESLLR
                     ILAEFGAKSSRVAEKKWDLSEEFLSKIAPIVVERLSVFGEFTAMLDAGEFDYFLEEPT
                     VDASKIVWKEEGPEKARDALLRVSEILEKISESNWNSEAVKEAIWPYAEERGRGGVLW
                     PMRYSLSGKDKSPDPFTIAGVLGREETLKRLKVAAENL"
     gene            8749..10059
                     /locus_tag="VHF05_02740"
     CDS             8749..10059
                     /locus_tag="VHF05_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVS79871.1"
                     /translation="MLKFRNIAILVAFCALIALTAAGIPKFAGAQAATADNSQTIKDL
                     NDQIQDHNQKIADLQKEIDQYQAQVDSTSKQANTLQSAVNSLNTTGKKLDSQIQLTQN
                     NIAAAELTIQKLELEIGDKEDQAKDMTNAIAETIRTMDQTDNISMIEVLLTRPDLSTV
                     WSDMESLSSLNDSLSSRVDLLRQVQEDLKGKQDATTEEQQQLASYQSSLSDKKQLVEV
                     NKQEKSTLLTQTKNQESQYKQLLAEKQAAKAQFEQELNQLQSKLQFTLNPNTIPPVGS
                     GVFRWPLDSVRITQYFGNTEFARTAAYNGQGHNGIDIAASIGTPVKAALDGTVIGVNS
                     QVAPMCQYGKWVLIKHANGLTTLYAHLSLVKVNSGDQVTTGQVIGYSGDTGYATGPHL
                     HFTVYASAAVKFEQYTCNSGITLTIPVSAFSGYLNPMDYLQPIP"
     gene            10071..10403
                     /locus_tag="VHF05_02745"
     CDS             10071..10403
                     /locus_tag="VHF05_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79872.1"
                     /translation="MEDYRDYGRSRRGGGIISALAVIVLVLIILKYVFNFDLLGLLND
                     PRVAPYFDKVYMPAANFWTAHIYPYAMIAWAYIVLGAELVWRFIQVALDKLQGFAGKF
                     YPQFLRPF"
     gene            complement(10744..11097)
                     /locus_tag="VHF05_02750"
     CDS             complement(10744..11097)
                     /locus_tag="VHF05_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79873.1"
                     /translation="MNLTEAGNSTALPNISTSAKTALVERVKKILSTFDGTKVDSTED
                     ISGTLDGKHIALTFKHVSHVLGSKIEEIIANDPHCSRFLAGEGEWRRDKGLTLRFRPH
                     EHIKVFEQECVGAVS"
     gene            complement(11189..11458)
                     /locus_tag="VHF05_02755"
     CDS             complement(11189..11458)
                     /locus_tag="VHF05_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79874.1"
                     /translation="MIDYWGENLTFFVKRMIAPILEDERVEEIVVKLHEAIPRGQQHN
                     ITRLLSGLRPGDSIVTISEGNTLSFLFGGLSMEERVSKMQFVPAC"
     gene            complement(11963..12409)
                     /locus_tag="VHF05_02760"
     CDS             complement(11963..12409)
                     /locus_tag="VHF05_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HVS79875.1"
                     /translation="MKKTIFLILLIVALIICITAFAQNAFKGLWNGGNSATTTESAAG
                     TEASTTASNGARQETAGEATSTAITSYDVSQHASKSDCWAIIEGKVYNATDLLASYSG
                     VEQGILALGCGRDDTELFESWAKANPNDASQLESAMKAYYVGDMLQ"
     gene            complement(12635..13246)
                     /locus_tag="VHF05_02765"
     CDS             complement(12635..13246)
                     /locus_tag="VHF05_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HVS79876.1"
                     /translation="MDYAIPVKEEILNAAQKALEANGFAVSIAENAGEAKSKVLALIP
                     EGAEVFTMTSMTLEALGIPKEINESGKYNSVRNKLNDPNTPAREKKRLGAAPEWTIGS
                     VNAVAATGSLLNGSNTGSQLGAYAYGADHVIFVVGAQKIAPSVDEAIKRIYDYVLPKE
                     TLRARAAYNLPEFNSFVSKLLITNREVIRGRTTIVLVKEAVGF"
     gene            complement(13267..13584)
                     /locus_tag="VHF05_02770"
     CDS             complement(13267..13584)
                     /locus_tag="VHF05_02770"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVS79877.1"
                     /translation="MSDLCDEIQKLGKGVSNAARYRILEAVAGGPKTVGDLTKVAKMS
                     QPAVSQHLKTLKACALVNDEKRGQEVYYSLNAEYTLRILKEFLVNLSKPAPKKSRAVF
                     RRT"
     gene            complement(13628..14251)
                     /locus_tag="VHF05_02775"
     CDS             complement(13628..14251)
                     /locus_tag="VHF05_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HVS79878.1"
                     /translation="MKGKKTFFIAVLLIAGGLLSLGYTLYHSVIADTGPGSQEVSSST
                     LAAAAGQAPPANFPEMNHPAELSIPSLGIDAKVVDVGVTAKGAMAVPKSFQQVGWYKY
                     GAMPGNSGSAVLAGHVDNGLSLAGVFKKLKNIGVGDAISVTDDAGDVATFEVTGTSSL
                     DYKSSDTSAIFKKGGPAVLTLITCDGDWVQSDKTYDARLIVTATRVK"
     gene            complement(14336..14602)
                     /locus_tag="VHF05_02780"
     CDS             complement(14336..14602)
                     /locus_tag="VHF05_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79879.1"
                     /translation="MARWGMAGAFMTLALLFASAFTVHAQTTTDQNASTTNTGTINTT
                     TGTGVGLPNTGAGGFSGANIAVILVSAAAIAYGIRLASRHYSYE"
BASE COUNT         3341 a         4109 c         3921 g         3454 t
ORIGIN      
        1 gagcacctgc actcgaattg ctaccgcttc attcctgaaa gctcgccttc ggcagaagct
       61 ccggtccaat cggctccatc ccaagccccc gcgtaacggg ggcttgttta attaggcggt
      121 caaaactgct aaaataagtg aaatgctgga agagcagtac aagatcaaaa cccaagtgtt
      181 cgaggggcct ttggagcttc ttctcgatct gatcgagaag cggaagcttt tcataaacga
      241 catttcgctc tcccaagtga cggacgactt tctctcctat atcaaggaga aggagaattt
      301 tccgctcggc ctctctgcca atttcattct cgtcgcttcg acgctccttc ttataaaatc
      361 gaaatcgctt cttccgtcga tcgagctcac cgaggaggag cagggctcga tcgacgatct
      421 ggagcgccgg ctcaaggagc ttgagcgcat ccgggagctt tccctgcatg tgaaaagcct
      481 tttcggcaag cgcattatct tcggccgcct gcaatcgagg gagcgcaagc cggtgttcgc
      541 gcccgaccct gcaatgacga tggcaaacat cgtcattgcg gcccagagcg tcttgaacag
      601 cctgccgaaa aaggaaatga agccggaggt gacggtgaaa aagataattt ccctcgaaga
      661 aaccatcgac aagctcactg cccgcatcgc caaaagcctc aaactgagct tcagggactt
      721 cgccgggcat gggacggatt cggctctgaa ccacgagaag aaagtcgaag tgatcgtgag
      781 ctttctcgcg atgctcgaat tggcgaaaca gggtataatt gccgtgagcc agaacgactt
      841 attcgacgat attcacatgg aaaccgaatc gatcggcgtt ccaaagtatt tctaatttta
      901 tgagcggcca tacttctgca ttgagcgcga gaatcgaagc attgcttttc tacaaagcgg
      961 agccggtttc gtacaagaag cttgccgatc tgctctcggt ttcccgcgag gagcttttgg
     1021 atggcttgaa cgagctcgaa aaagccttgg aaggccgcgg catccaactt gtccgcaatg
     1081 acgaggaggc gtttctaggc acgtcagccg ccgcctccga gctcatcgag gcgatccagc
     1141 gcgaggaact ctcccgcgat ctgggcaaag ctggcctcga gacgctcgcc atagtactct
     1201 ataagggtcc ggtcactcgc cgggagatcg actatgtccg cggcgtcaat tcggcgttca
     1261 tcctgcgcaa tctcctcgtg cggggcctgg tagagcgcgt agaaggggag ggcaggggat
     1321 attcctaccg tccgaccacc gaccttctat catttatggg aatcggctcg cgaagcgatc
     1381 tgcccgaatt tgctgcggtg cgcgccgaga tggagaagtt cgcggcttcc gactcggaag
     1441 gatcggaggg aaaagaagag aagagcgatg atgcggcata aaaaatcaag ttacatattc
     1501 aaaaaacgga gaaagaagat gtcattcctc actgcgctcc aaaagcaaaa gttcctgtcg
     1561 ctcacgctct tggtgggcgt cctcatgacg ggatacatgt tcctgcgcca tatcgatccg
     1621 aacatccacg cgccctcgct ttcgagaaac accgcagcgg ccgaggcttc gcttccggcg
     1681 accaccactc ctccgaacgc ctttaagaac gttcaaatcg ccgccaaggc ggcgatcgtg
     1741 ctcgatgcca ggagcggaga agttttgtat gaaaaaaacg ccgacacggc gcttccgctc
     1801 gcttcgctca ccaagatcat gtcggcgatt accgcggccg atctcgcgtc gatgaattcg
     1861 acggtgacgg tcgatcagtt cgccctcgag acggacggcg acaacggcct gtatgcgcaa
     1921 gagagctgga agctcaagaa tcttctcgat ttcagcctta tcgtctcgtc gaatgacggc
     1981 attaccgcca ttgcttcttc ccttggcggc actattctcg gcgaaacggc gtctgccgcc
     2041 gcggctgatc cggcaagcgc ggaagcggcg ttcgtcggtc ggatgaacaa cgaggcggag
     2101 aagctcgatc ttcccacgct tcgcttcatc aacccgaccg gcctcgacga gggcgtgaag
     2161 accggcggca tgggctcggc gcgcgacgtc gcgaagctcc tctcgtacgg catttctgac
     2221 tatccttcgg ttttcgaggc gacgcgctac cagtcgatcg acgtaacgtc cgaaaacggc
     2281 atcacccaca cggcggtgaa caccgacgcg gttctcaatt cgattcccgg cctcatcgca
     2341 tcgaagacgg gctacaccga cctggcgggc ggcaatctcg cggtcgcgtt cgatgcggga
     2401 ttgaaccgtc ccgtcgtggt ggtggtgctg ggctcgagct acgacggacg cttcggcgac
     2461 atggcgacgc tcgtcggcgc gacggaggat tacatagccg gaagcacgga caaggagtcc
     2521 ctcgcttcca ataaggaata atcatgatca tcgatatcgt caaagtattc atcccgccgg
     2581 tcgtcgcctt catcctcggc gtcgtactga cgccgattct caccaactac ctctacaaga
     2641 acgagatgtg gaagaagaag gcgggaaaga agacgttcga cgggcaggag acgccgatct
     2701 tcaacaagct ccatgaggga agggaagtgg gaacgccgaa gatgggggga atcatcatct
     2761 ggttctcggc ggccgtgacg attatcggca tctgggcgct ctcggcgatt ttcccgaccg
     2821 agaacacgct gaagctcggc ttcctttcgc gaagccagac ctggattccg ttcggcacgc
     2881 ttcttttggg cgcgctggtc ggactcattg acgattattt ggatatcaaa ggcaccggca
     2941 aacatgtcgc cggcggcctg ccgctcaaac agcggctctc gatcgtcgcg attatcggcc
     3001 ttctctgcgg cggatggttc tacttcaaac tcgccgtctc gtcgatcggg cttccgatga
     3061 acctcgtcct caatatcggc tggctcatca ttccgctctt cgcgatcgtg acgatggcga
     3121 tctactcggg cggcgtgatc gacggcattg acggcctggc gggcggcatc ttcgcgtcga
     3181 ttttcgccgc ctatgcgggc atcgcgtttt atcagcagca gatcgatctc gccgcgttct
     3241 gcctctgcgt cgtcggagcg atcctcgcat tcctctggtt caatattcct cccgcccgct
     3301 actacatgtc cgaaaccggc tcgatggcgc tcaccgtgac gctcgcggtc gtagccttca
     3361 tgaccgactc gcttgccggc ggctacggcc ttctcgtcct tcccgtcatc gccttgccgc
     3421 ttgtcgttac cgctgcctcc gatatcatcc agatcacttc gaagaaattc cgcggcaaaa
     3481 aggtattcct cgtcgctccg atccaccacc atttcgaggc ggtcggctgg ccctcgtaca
     3541 aggtcactat gcgcttttgg gtcattggcg tggtatcggc catcctaggg gtcattttgg
     3601 ccctcctagg ggtagggaaa tagcctttct tgggagaggt ttatccgggc tcgccccttg
     3661 ggtccgattt tccgttctca caacttcatt gacgcttcat aaaaacgggc atacgattga
     3721 aatagaagga gtttgagcaa tgggatgctg gttaagttca gctcacgcat accgcatcac
     3781 atacgctgat gcgcgctata cgctgaccga ttcgcatatc aaacaggaat ttcacgctgc
     3841 ttccggcaaa ggcgaatggc tttccggaaa cgagtaccct atacaaaaga atgtgacggc
     3901 cctcgtgcct cctttcggct tatattgccg ctataggatt atcgaaggcc acccgctttc
     3961 ctgccttgtg ggagacgcgg aaacggaaaa caggctgttt gcagtcgttg agacctacga
     4021 ctatgacaag gaggggaatg cgaagaatcc ccacaccatc ctagtcacgc ttgaagaaac
     4081 ggatacctat ccgcttcggg tcaagatgag atttgatcat caaacccgcc aattgactgc
     4141 cgatcctaac tgccagattg aaaaattcaa agcatggatc aagcttctga atcaagatct
     4201 caattggagc cggcattcga gaaaagtcca cgattcgaaa atgccccaat cagttccgaa
     4261 ttacgcccaa gccaaggctg tttagcctgc cgaaagaagg acagaccgtc gcgcctgtcc
     4321 tttttcttat cctcattagt ctattggtgg aaagctaatg cggagctaca atgatatgca
     4381 gagggaggag ctgccgcgta tgcactcgat gagttttgga atgaagaacg gcaaaggata
     4441 cgtgacgtat tgcgggaaga tgtacccggt cgaaaaccag gatgattgcc ggttcggatc
     4501 tgtattcggt ctcggcatgc acattgaagt atgcgtgctt gggcacaata acgatctgcc
     4561 cggagaaaag ataaccgact ctaagggccg caaagtcacg ctttgcagaa tcgagaggcc
     4621 gatagttgcg gcataggtaa aaaacatgcc gcacgcaatc ataattattt atcccgtcgg
     4681 gattcggaaa ggcggccgcc gtgccgcctt ttatttttta gaaaggggcg gccgtggggt
     4741 ataatgcaat gaagcaattt agcgcctgag caatgaatcc cgttagagac acatcagttt
     4801 agtcgcggct tttaatttca gaaagattat gaaagcggat atttccatta ctagcggcat
     4861 aacatcggaa atgcagtgca tttcctatct ctaacgggat gaagagagcc ggtaaaatcg
     4921 acaagccatt tcttatcgcg gtgattattt tggtcgtagc cggctatttc attttcagct
     4981 cggcttcttt gggcctcttg gcgagccagc aggccaaata ctcgaacgtc gccttcaacc
     5041 agacattttt cggcctcttc ctcggctcga tagcctgtat catcacctcc cgcctcgact
     5101 atcgcatctg gaggaagtat tcgctcatcc ttttcgtcct ttcggcgatc atgacgcttc
     5161 tcgtcttcgt gccgcatatc ggattctccc acggaggcgc gacgcgctgg atcaatctcg
     5221 gcgttacgac cttccagccc tcggagctcc ttaagatttc gtttattttc tatttctccg
     5281 catggatcgc caagtacaag gacaaggtga attcgttcaa atacggcttc ctgccgtttc
     5341 tcgtgcttat cggcatcgtg ggcgccatcc tcatcaagca gcccgacacg ggaaccttcg
     5401 ccgtaacctt cgcggcgggg cttgcgatct acctcgtcgc tggcgcctcg tggctctatc
     5461 ttctcctgct tgtcgcagga tcggccctgg ggctcttcgt catcgcgaag ctccgcccgt
     5521 acgtcatggc gcgctttacg acgtttctcg atccctcggc cgacgcgctc ggcgccggct
     5581 accaactcca gcagtcgctt atcgctatag gctccggccg cttcctcggc cgcggcttcg
     5641 gccagagcat ccagaagttc aattatctgc ccgagccgat tggcgactcg atcttcgccg
     5701 tgatgtcgga ggaattcggc ttcctgggaa gcgtcaccct cgtcctcctt ttcctctttt
     5761 tgaccgttcg ggggctcaaa atagccgcca cgagccccga tacattcggt agactggtga
     5821 ctgtcggtat tgttatactt atcatatctc aggcgttcat aaacatgggc ggaatgctcg
     5881 cgataatgcc cttgaagggc atcaccctgc ctctggtgag ccaaggcggc acggcgctct
     5941 tcatgacgct cgcggagata gggattatcg tcaacatctc gaaattttcc aaggaatcca
     6001 aagaatcggc gtaagctcat atgaatcccg ttagagatcg gaaaattgga aagatgaagc
     6061 gtgttgcaat caaaaatgaa atcgaaagct tttgaaatta aaaacataac tacggaaacg
     6121 gccgccgttt cctatctcta acgggatgaa aatacttttt acaggaggcg gaaccggcgg
     6181 acatttttac cccatcgtcg cggtagcgga ggaaatcaag aagcaagtcg ttgaaaacaa
     6241 gctcgtcgag gcggagctct atttcatgtc gccaacgccg tataatgcgg gcatcctcta
     6301 cgagcgcggc atccgcttca ttcccgtggc ggccggcaag cagcgcacct acttctcgat
     6361 tctcaatttc ttcgatctct tcaagacggg atgggggatc gtgaccgcct tgtggaaagt
     6421 tttcaacctc taccctgatg tcattttcag caagggcggc tatacgagct ttccggtgct
     6481 tgtcgccgcg cgcatcctcc gcattcccgt cgtcatccat gagtcggatt ccgtgccggg
     6541 caaagtgaac cagtggtcgg gaaagttcgc cgcgcgcatt gccgtttcgt tccccgatgc
     6601 ggcagcctat ttcccgaagg atcgcgtggc tctcaccggc aatccggtgc gcacggaaat
     6661 cgccctgcgc tccaccgaag gcgcgcgcga attcttctcg ctcgaaccca atattcccgt
     6721 catcctggtg ctcgggggat cgcagggctc gaagatcatc aacgattcga tgaccgacgc
     6781 cttgccggag cttctgaaga aataccaggt gctccatcag tcgggcaaga acaatttcgt
     6841 cgaagcgaag gaaacggccg acgtcgtgct tttgaatgac gacaacaagc cgcgctacca
     6901 tctcgtcgat tacttcaata ccgtggcgct ccgccatgcg gccggatctg ccgacctggt
     6961 gatttcgcgc gcaggctcga ctatcttcga aatcgccgcg tggggattgc ctagcatcat
     7021 cattccgatc cgcgaatcgc ggaacgacca ccaggcgaag aacgcctacg cctacgcccg
     7081 cgcgggcgct tgccaggtcg tcgaggaaat gaatctcacg tcgaacattc tcgccgccga
     7141 agtgagccgc atactcgaca atgtcgaact ccgcgggaaa atggcggaag cagctaaggc
     7201 gttcttcagg ccgcgcgccg cggaagcgat cgcgctggaa attttgaaga tcgccgcaca
     7261 gcacggcgga tagaatttat ggacacgaac aaccgaaaca ttgttacgcg ttttgcgccg
     7321 tcgccgaccg gcttcatgca catcggcggc gtgcgcacgg ccctcttcgc atatctctgg
     7381 gcccgcaaga accggggcac gtttatcctg cgcatcgaag acacggacaa ggagcgcgag
     7441 gtggcgggct cgatcgagca catccaggaa tcgctccggt ggctcggcct cgaatgggat
     7501 ttcggaccgg acaagccggg aacgttcggc tcctgcatcc agtcggagcg ccttccgacc
     7561 tatctcgaat ttgcgcatcg gcttatcgag aagggcctcg catatcctga tccgtattcc
     7621 tccgagcagg tcgctgaatt ccgcaagcag gcggaagccg aaaagcggcc gttccttttc
     7681 cgcaatcatc ggcccgaagc gttcgaaaag tgggacagca cgaagccgct ccgtttcaag
     7741 gttcccgaga tcaagcgcta tcgttggcat gacgccgtcc gcggcgatct cgaggcgggc
     7801 gaagaagcgc tcgacgactt cattctcata aagagcgacg gctatccgac gtacaacttc
     7861 gcccatatca tcgacgactt cagcatgggc gtcacccaca tcatgcgcgc cgacgaattc
     7921 atttcgagca cgccgcgctt tctctcgctc tacgaggcgc tcggcatcgc gccgccggtc
     7981 ttcgtgaccc tgccgccgat ccttcgcgac gaccgcacca agaagctcgg aaagcgcgac
     8041 ggcgccaagg acattctcga ctaccgcgcc gaaggctatt tgcccgaagc gatggcgaac
     8101 tttcttgcgc ttacgggatg gaatccgggc accgagcagg agattttcac gatggacgat
     8161 cttatcgccg cattcgacat cgcgaagatc cagaaagccg gcgcggcatt caacgaagag
     8221 aagctcttat gggcgaaccg cgagcatatc aaaaatctct cgcatgagag cctgctccgt
     8281 atccttgccg aattcggcgc gaaaagctcg cgcgtcgcgg agaaaaaatg ggacttgagc
     8341 gaagaatttc tctcgaaaat cgcgccgatc gttgttgagc gcctgagcgt ttttggcgaa
     8401 ttcactgcca tgctcgatgc tggcgagttc gattacttcc tggaggagcc gacagtcgac
     8461 gcttcgaaga tcgtctggaa agaggaaggg cccgaaaaag cacgggacgc ccttctgcgt
     8521 gtttcggaaa ttcttgaaaa aatatcggaa agtaattgga attcggaggc tgtcaaggag
     8581 gcaatctggc cctacgccga agagcggggt aggggaggcg tcctctggcc catgcgctat
     8641 tccctctctg gaaaagacaa atcccccgat cccttcacga tcgcgggcgt gctcggcagg
     8701 gaagagacat tgaagcgcct caaggtggcg gcagaaaatt tgtaaatcat gttgaaattc
     8761 aggaacattg caatattagt cgctttctgc gcgctgatcg cgctcacggc ggctggcatt
     8821 ccgaagtttg cgggcgcgca ggctgctact gcggataata gccaaacgat caaggatctg
     8881 aacgaccaga tccaggatca caaccagaaa atcgccgacc ttcagaagga aatcgaccaa
     8941 taccaggcgc aggtcgactc gacaagcaag caggccaata ctcttcagag cgcagtgaat
     9001 tctctcaaca cgaccggcaa gaagctcgat tcgcagatcc agcttacgca gaacaacatc
     9061 gccgccgcgg aactcactat ccaaaaactt gagctcgaga tcggcgacaa ggaggatcag
     9121 gcaaaggata tgacgaatgc cattgccgag accatccgca cgatggacca gaccgacaat
     9181 atttccatga tcgaggttct tctgacgcgt ccggatcttt caaccgtatg gtcggatatg
     9241 gaatcgcttt cgtcccttaa tgacagcctg agctcgcgcg tagatctgct ccgccaggtg
     9301 caagaagacc ttaagggcaa gcaggatgcc acgacggagg agcagcagca gctcgcgtcg
     9361 taccaatcgt cgctttccga caaaaaacag cttgtcgaag tgaataagca ggagaaatcg
     9421 acgcttctca cgcagaccaa gaaccaggaa tcgcaataca agcagctgct ggccgaaaag
     9481 caggccgcca aggcgcagtt cgagcaggaa ttgaaccagc tccagtcgaa gcttcagttc
     9541 acgctgaatc cgaacaccat tccgcccgtt ggaagcggcg tgttccgctg gccgctcgac
     9601 tcggtgcgca tcacgcaata cttcggcaac accgaatttg cccggacagc cgcatacaat
     9661 ggccagggcc acaacggcat cgacatcgcg gcctcgatcg gcacgccggt gaaggccgcg
     9721 cttgacggca cggttatcgg cgtcaattcg caggtcgcgc cgatgtgcca gtacggcaaa
     9781 tgggttctca tcaagcatgc caatgggctc acgacgctct atgcgcacct gtcgctcgtg
     9841 aaggtcaatt cgggcgacca ggttacgacc ggacaggtca tcggctacag cggcgatacc
     9901 ggctacgcga ccggcccgca cctgcacttc acggtctacg cctctgcggc cgtcaaattc
     9961 gagcaataca cgtgcaacag cggcatcacg ctgactattc cggtatcggc attcagcggc
    10021 tatctcaacc cgatggatta tctccagccg attccgtaaa ataattagtt atggaagatt
    10081 atcgagatta tggaaggagc aggcgaggcg gcggcatcat aagcgcgctt gccgtcattg
    10141 tgctcgttct tatcatcctc aaatacgtat tcaatttcga ccttttgggg cttttgaacg
    10201 atccgagagt cgcgccttat ttcgacaagg tctacatgcc ggccgcgaat ttttggactg
    10261 cgcacattta tccgtatgcg atgattgcat gggcgtatat cgtgttgggc gcggaactcg
    10321 tatggcggtt catccaggtc gctttggata agcttcaggg atttgcaggg aagttctatc
    10381 cgcaatttct ccgtccgttt tgattttctt tttgccattt gattgttggt taagactaaa
    10441 cccgaaaatt tttaaatgaa ctgcagggaa gtggttctca aaggaactgc ttcctttatt
    10501 atatattagt gtcgtaaaag atatattgtg cgacattagt atatactgga gatactgaga
    10561 atcaaccgct cccctctatc catcatcatg cgcatctttc ctttaatttt aaaaatacga
    10621 tttaggatca aaattatttt tcaaattaaa attttttagg aattcatttc cctggtggat
    10681 tcatctggat ataaaaatgg ccggaagcat attcgcttcc ggccgttcta agtgccgcca
    10741 cggttacgag actgcgccga cgcattcttg ttcgaacacc ttaatgtgct catggggtcg
    10801 aaatcttagc gtgaggccct tgtcacgccg ccattcgcct tctccagcga ggaagcggga
    10861 gcaatgcgga tcattcgcaa tgatttcctc gattttgctg ccgagaacat gcgacacatg
    10921 cttgaaggtc aaggcaatat gcttgccgtc gagcgtgcct gatatatctt cggtcgagtc
    10981 aaccttcgta ccgtcaaagg ttgaaagaat tttttttacc ctttcaacca atgcggtttt
    11041 tgcggatgtg ctgatgttcg ggagtgcagt gctgttgcca gcttcagtaa ggttcatact
    11101 ggcttcaaat taccagctat acctatattt gtcaatgtaa aagcccagcg gtatggccgc
    11161 tgggcatgtt gtaagccggc attctcccct aacaggcagg gacgaattgc attttggaaa
    11221 ctctctcttc catggagagg cctccgaaca gaaaggaaag agtatttcct tccgagatcg
    11281 tcacaatact atcgccggga cgaagcccgc tcagcaggcg ggttatatta tgctgctgtc
    11341 ctcgcggtat agcttcatgc aattttacga ctatttcctc gacgcgctcg tcctcaagga
    11401 tcggtgcaat catacgttta acaaagaatg tgaggttttc gccccagtaa tcaatcactc
    11461 tcgtcataaa gctctcctga aatgacaata ccacataatc taaaaatatc aactcaccgc
    11521 aagaggctgt ttggttccaa tataaaaggc ccgactatac agcgtcggac ctttgcgggt
    11581 cggagccgcc cgtattccag gacggcggcg accatgcttg tatgcgcgga tacatatacg
    11641 gattggggct ttttactcca atctcgatat catacgtatc tctcatccta tttatccaaa
    11701 gaccagcata gtctttgaac aatgacacta tcctttcccg gtgatccact gtgggacacc
    11761 ggataatggc tcgataagct ttgctataaa aaaacgattc tgaaacgtag ttgtaaatct
    11821 cttccaggct cacatcatct agacggccgt cagcattcac cagtaggggt ttcattcgat
    11881 tatctccatt tccagaatag catgattaca ttgccatgtc aattacctgg caaatgagag
    11941 aatatgggct gttgtaaagg ctttactgaa gcatgtcgcc cacatagtag gccttcattg
    12001 ccgattcgag ctggctcgca tcattggggt tggctttggc ccagctttcg aaaagctcgg
    12061 tatcgtcccg gccgcagccg agcgcgagta ttccctgctc gacgccggag taggaagcga
    12121 gaagatcggt cgcattgtag accttgcctt ctataatcgc ccagcaatcg gatttcgagg
    12181 catgctgtga cacatcgtat gaagtgatgg cagtgctggt ggcttctccc gcggtttctt
    12241 gccgcgcgcc gtttgaggcg gtagtggaag cttcggtgcc tgccgcgctc tcggtcgtgg
    12301 tggcggaatt tccgccattc cacaggccct taaaggcgtt ttgggcgaat gcggtgatgc
    12361 agatgataag cgctacgatg agcaggatga gaaatatggt ctttttcatg cgcgcattat
    12421 accaggccgg ccgatattgc gcgcaaggcg tcataatcct ctaattcttt aagcatgacg
    12481 cccgcttaac ctactttttc atgcgcgtcg tatactattt cctcagaacc cggcaaggct
    12541 ttccggtttt gtttctcact caaccaagcc ttgttttgca aaaagaatgc cgcgagctgg
    12601 atcggctcac ggcgttcttt tcatatcata attcctaaaa tccgacggct tccttgacga
    12661 gaacgatcgt agttctcccc cttattactt ccctgttcgt gataaggagt ttggaaacga
    12721 aggaattgaa ttcaggaagg ttgtaagcgg ctcgggcgcg cagagtctcc ttcggaagca
    12781 cataatcgta aatgcgcttg atagcctcgt cgaccgaagg cgcgatcttc tgcgctccga
    12841 caacgaagat tacgtgatcg gcgccgtagg cgtacgcgcc gagctggctt ccggtgtttg
    12901 agccgttcaa aaggcttccg gttgcggcta cggcgttcac gctgccgatc gtccattccg
    12961 gcgccgctcc caggcgcttc ttttcccgcg cgggcgtatt cgggtcattg agcttgttgc
    13021 ggacggaatt atattttccc gattcgttga tctccttcgg gatgccgagc gcttcgagcg
    13081 tcattgaggt catggtaaag acttcggctc cttcgggaat aagcgcgagg actttcgact
    13141 tcgcttctcc ggcattctcg gcgattgaaa cggcaaagcc gttcgcttcg agcgcttttt
    13201 gcgccgcatt caaaatctcc tcctttacgg gtattgcgta atccatatag ctctttcgtt
    13261 aaatgattaa gttcgccgga aaaccgccct gctctttttc ggagccggct tgctcaggtt
    13321 cacgaggaat tccttgagga tgcggagggt gtattcggca ttgagcgaat aatatacctc
    13381 ctgccccctc ttctcgtcgt tgacgagggc gcacgccttg agcgttttca ggtgctgcga
    13441 taccgcgggc tgggacatct tggcgacctt cgtgagatcg cccactgtct tcggccctcc
    13501 cgccactgcc tcgagaatcc tgtagcgtgc ggcattcgat acgccctttc ccagcttctg
    13561 aatctcatcg cataaatccg acatataaat atattagcat ttgcttatat gaatgcaact
    13621 agccgcgcta cttcacgcgt gtcgcagtga cgataagccg ggcgtcgtag gtcttgtcgc
    13681 tctgtaccca atccccgtcg cacgtaatga gcgtcagcac ggcggggccg cccttcttga
    13741 aaatagccga ggtgtcggag ctcttgtaat cgagcgagga tgtccccgtc acttcgaagg
    13801 tggcgacgtc tcctgcgtcg tcggttaccg aaatggcatc ccctaccccg atgttcttga
    13861 gtttcttgaa cactcccgcg agcgagagcc cgttgtcgac atggccggcc agcaccgcgc
    13921 ttcccgaatt tcccggcatg gctccatatt tgtaccaccc gacctgttgg aagctcttgg
    13981 gcaccgccat ggcgcccttg gcggtcactc ccacgtcgac gaccttcgca tcgatgccga
    14041 gcgagggtat ggagagttcg gccggatgat tcatttcggg aaaattcgct gggggcgcct
    14101 gaccggccgc ggccgcgagc gtggacgacg atacttcctg cgatcccggc ccggtatctg
    14161 cgatcaccga gtgatacagc gtataaccga gcgaaaggag gccgcccgcg ataagcagaa
    14221 ccgctatgaa aaatgttttt ttgcccttca tcttctttct gcgtgttttg atccgccgaa
    14281 caggcagcat taaaacaaat acctccggag aggtattcgt ctgtgcctcg gcgtattact
    14341 cgtagctata atgccgcgat gcgaggcgaa tgccgtatgc gattgccgcc gccgaaacga
    14401 gaatgaccgc tatgttggct cccgagaatc cgccggctcc ggtattcggc agtccgactc
    14461 ccgtacccgt ggtggtattg atcgtgcccg tattggtagt cgatgcattc tgatcggtgg
    14521 tagtttgcgc gtgcaccgta aatgcggagg cgaagaggag cgccagggtc atgaatgctc
    14581 ccgccattcc ccaccttgcc atatggttca ttgttttcat aatgcgaacg cttttaaatt
    14641 attaattgta aaggacgcgt gcctttgaag gccacacaac tctacaaccg cagtataaaa
    14701 aaaggatgaa tggcatttta aggtttggat gaacgattct taatcaatgc atgaatttgt
    14761 cctaaaattc attccatcgg aacgtggggg tgaaatattg ctttggagaa acattgcaca
    14821 ggcga
//
LOCUS       DASZYS010000045        25693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_873855, whole genome shotgun sequence.
ACCESSION   DASZYS010000045 DASZYS010000000
VERSION     DASZYS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 25693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25693
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873855"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VHF05_02785"
     CDS             <1..915
                     /locus_tag="VHF05_02785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79880.1"
                     /translation="VQNDEYWGEFPEKDLDVVLDKYRRGDSAAARARITELGRDDFVF
                     GFARSDFLYGLDIGPGKRVLDIGCGLGVHSFNAARTGAEVYGCDLSSKRAEFCMLRAK
                     SDGVENAHFFHSDILNLPFAAESFDAVVLNGVVEWLGEMNRNKDPRQDQIEGLKSVLR
                     ILKPGGTLYIGIENRIAATYLFHATDHNRLKYTTFMPRFLADFVTRRKKGKSYRTYTY
                     AKGGYEKLLRDSGFSNPACFYIAHPGYNLPQYLIPFGDTAALCFVLNSMSIDKGMRGA
                     FVRAIAVLPGMGKITRNFFYSYAMYVKK"
     gene            912..1748
                     /locus_tag="VHF05_02790"
     CDS             912..1748
                     /locus_tag="VHF05_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79881.1"
                     /translation="MIDQITDIVKKNWEKIPEAARAGADPERISVTKFHHGRALSKSK
                     VLFLVTSKDKLVCLVKAARTAEGNEKLRRERDAQVQFGSTQLLKTPAVYFDGLINGSF
                     FYAEEVVDGRPLSLSEAQSRESEIIAMIASWPAEGTVSTSKIAEVFGSAMKEEDTDFK
                     NLHNSLTNLNANLKKGMTHGDFTRKNILKHAGGFYLVDWDRAFERPFWLLDAVHYLVR
                     SRDIKNFEEWKEKGSPILIKEALVDEPAVSALYLAESLLELLYKRSPESYLRALEGFS
                     RL"
     gene            1880..2935
                     /locus_tag="VHF05_02795"
     CDS             1880..2935
                     /locus_tag="VHF05_02795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVS79882.1"
                     /translation="MKGEKERSAFFAAQGINEPFPIVSLSSAANRYKDSENVWLIRLS
                     VLLSNFSLVREVWARRRAFDVLYARDHQLLLPIAFAKFALRKRVLFESHFILAKRGSQ
                     FAVELVARMADGIVAITQNLQAYYSRFTPRTIVSYCAASEPQRFSSISESVSELRKAL
                     DLPQEAIIICYTGNMAYTGTGHSYGIEDIIRSLPLLPEKFVFVGVGRKENERLLPEQI
                     AYDLGVENRALFRGWVTRDEVTRHIVAADILVVPASGAQPGNSPTKMFEYLAAGKLIV
                     AANTEAIAEVLRDGENALMVDYKNPQAWADAFGKIVNDAGLSGKLAEHARADSQFYSW
                     EKRGETIVGFISDTIHE"
     gene            2928..3887
                     /locus_tag="VHF05_02800"
     CDS             2928..3887
                     /locus_tag="VHF05_02800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79883.1"
                     /translation="MNNIKRIILRTAARMGLNELAGSTASQSAFCIGYHSIRSRRNSK
                     ELSGELYWNLSIDEEEFDAQITYLKKHGHTFVTVGNLLNAVKKGIKKPTAIYFDDGYR
                     DNLLNALPILKKHAVPATVFVTTGAIDRTCLLWTLSLRNLYRKIGKENVEEEIAKLKD
                     LLAHEAAERVDEVYRAHGLKLDSDDFDMFLSWEEVGRLAQEGIEIGSHTVTHPNLTKV
                     DAARMNSELVESKSRIEEKIGVSVGSLSYPYGRVNGEVADAARAAGYRCAIGGVEGWN
                     SFRTIQSRRFELRKIAPRPGGDIVDFATRMYLFPLRKRISEFL"
     gene            3892..4719
                     /locus_tag="VHF05_02805"
     CDS             3892..4719
                     /locus_tag="VHF05_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVS79884.1"
                     /translation="MNKILDNFKRKFLQFHHAKKFSSLGANWQANTVLFFFYLYSRLA
                     LRFELTYLPQTKIKLRQFGKEFEFIFTHPTDIPVFNHIFFEGEYEISDLKKEPLIILD
                     LGSSVGISVLYFHLKYPKAVIHAFEADPNTFRILKENTKNIENIKVNNICLSDRDGEK
                     ELFISDTSVSSSVVLRSKNQQSVLMTAKGLDSIIKEVVSTQVIDLIKFDIEGAEFEVL
                     SASKEKSQVAYMVGELHLDLIEGSLENFLAQFSDFKVSLKKNSSQRYAVYMKNKNLI"
     gene            complement(4712..5986)
                     /locus_tag="VHF05_02810"
     CDS             complement(4712..5986)
                     /locus_tag="VHF05_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVS79885.1"
                     /translation="MNSIRTKLNNMVVRIGLILGFDSKYVFSGTFWMTVGQTIAALTS
                     FCLALAFAHFLSKDTYGNYKFVLSIATLLSSLTLSGLGTVIVQAAARNQDGFLQKAFA
                     LNLKWSTFFTVAMVVVAAYYYAQGNHALAVSVLIAGFLAPVFASANFYDSFLAGKKAF
                     KASALLDGLSVAVPAAILIGCMILTQNFVILILAYYISNTASNLAIYAYVARAYKKND
                     DMDSASIGYGKHLSLMNIFSAVANNIDQILIFHYLGAASLAIYAFAIAIPEQLKGVIK
                     NVGNLALPKFSERDAHDINATIWKKILLFMLVVVLGVAFYIAVAPVIFRIFFPQYLES
                     LAYSQIYSLSLIVTVSVIPSSALQAQSAKKELYASTIYSSLFQITLIFGFVYFYGLWG
                     VVWARVITRGTTFLISAGYWNAFTKRTSVQLK"
     gene            6056..8248
                     /locus_tag="VHF05_02815"
     CDS             6056..8248
                     /locus_tag="VHF05_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVS79886.1"
                     /translation="MKFKTILRYIVLAGIFVVPFLPFFVANSMYFPFISGKNFAFRII
                     IEIIFACWLILALSDKSYAPKKSPIMWAYSAFMAIVILADIFGVNPYRSFWSNYERME
                     GLIAHLHFFAYFLVLGSTLSGQKLWDAFWKTSIGASLIMVVYGFAQLGGVAAIHQGGV
                     RLDGTFGNAAYLAVYMLFNIFLSAYYLAHTWKKPAERWIYMVTIPLQFIILFYTATRG
                     SLLGLLGGVFLSAVLLVIAERKRKKLRNISIGIGAALIVLVLVFIAMRNVPFIKNNEV
                     LGRFASISLHDKTTESRFMIWNMAWQGVLERPVLGWGQENFNLVFNKFYNPKMYDQEQ
                     WFDRTHNIVFDWLIDAGFLGAIGYFALFGLIIYGIWKTKQFSSLEKSILVGMMAGYFV
                     HNFFVFDNLVSYIIYFSIAGWIYSLQTEEKRAAAAGKPMMNMEVVNYGLAPVAAVALI
                     LCVYFLNTRPIQANETLIQALQNEKDPQTSLMYYQKAIGYGTFGSGEARERLVIDAPN
                     MAANASADVKSQVLSYAASQMEEQIKAAPNDARYELFLASFYDGIGDYDDALVHVNRA
                     LEESPKKQAILIEKGVAYIGKKDYQDALAAFKEAYDLEPDYTDAKTIYAAGAAYAGDK
                     DLAMSLLSGLSDQDLLTDDRALGAYAQLGDYDKVLQIYQLRLANHPEKDDARAQIELS
                     IAATYMQLGRKTDAIAALQQIASESPQYASQIQQYIDDIKAGKNPVQQ"
     gene            8299..8667
                     /locus_tag="VHF05_02820"
     CDS             8299..8667
                     /locus_tag="VHF05_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVS79887.1"
                     /translation="MASIETRKNGIFDLEERTAKFGEAVIAFCRKLPKDEVTRPLIVQ
                     LVKCGTSVGANYCEADDAESGKDFKHKIGICKKESREAKHFLRMSAVAVPEYKNEARK
                     LWLEAKELNLIFNSIYKKVK"
     gene            complement(9072..9341)
                     /locus_tag="VHF05_02825"
     CDS             complement(9072..9341)
                     /locus_tag="VHF05_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79888.1"
                     /translation="MPFFSHLIFKLKHEPNDLRTLLVDRHTITLQKYPVDLIFRPAEK
                     HLIAHFHALLGIFDGWVIKWFATSVAVWIEPVRISCHFTHYALNV"
     gene            9335..9709
                     /locus_tag="VHF05_02830"
     CDS             9335..9709
                     /locus_tag="VHF05_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79889.1"
                     /translation="MAWEKGYPDVYRSILSFILAEYAHTDNYRVWEEIEFQRLLQRQS
                     FWNEKKTEFARELYIILAPKMAKWVVDCGLFIFGYLVREFWNKIVDLNKKRGAPLEDE
                     IWVASIFHLSINVMAPMKYMLP"
     gene            9749..10000
                     /locus_tag="VHF05_02835"
     CDS             9749..10000
                     /locus_tag="VHF05_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79890.1"
                     /translation="MSEVDRPQYSEEVLQELKMLCESFGSGEEARNDPNFVLTSLLKR
                     LNERKDSISAMTRTDLEWSITPQSLKFIRSQAAEQKSAA"
     gene            10845..13364
                     /gene="secA"
                     /locus_tag="VHF05_02840"
     CDS             10845..13364
                     /gene="secA"
                     /locus_tag="VHF05_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVS79891.1"
                     /translation="MDFLKNLFGDDSSREVGRIMKIVEKVNAFEPEISKLSDEELRGK
                     TAEFKDRLAQGATLDDILPEAFAVVREASQRTLKQRHYDVQLVGGVVLHEGSIAEMRT
                     GEGKTLVATLPTYLNALTGKGVHVVTVNDYLSRRDAVWVGQIPAFLGLTVGVITTDGS
                     YIYDPAHKDLDQERDEVGAFKVVHEFLRPVSRREAYEADITYGTNNEFGFDYLRDNLA
                     YSKDQVRQRGYHYAVVDEIDSILIDESRTPLIISMPAQESERLYETFADIVSKLKVDE
                     DYTVDEKLHAIQMTDEGITKAEKMLGVENIYTDRGIKYVHHLETAVRAKALYTRDKEY
                     VVRDGEVIIVDTFTGRLQPGRRWSEGLHQAIEAKEGVHVQKESRTAASITFQNYFRFY
                     EKLSGMTGTAKTSAEEFYKVYELPVVTIPTNKPTIRKDDNDLIFQTENGKFRALARKV
                     KELNEKGQPVLIGTVSIEKNELLSEYLNKEGIKHTVLNAKNHEREGEIIAQAGQKGGV
                     TVATNMAGRGVDIKLGGSPSTDEEYEEVKNLGGLYVIGTERHEARRIDNQLRGRAGRQ
                     GDPGETQFYVSLEDSLMRVFGSDRIKGMMGRFGIPEDEPIENRLINRSLESAQEKIEG
                     FHFDARKHVLEFDDVLNYQRKIVYERRRAILFADKAELTAHIQSEVFDPAPDEIRPGL
                     EAKRRQLGEDLFLEAMRAVILQTIDMYWIDHLETMDYLRNSVNLRAYGQRDPLVEYKR
                     EGLRLFKEMQDAIRDQTWAVIPNIGGPVPTEEEKPLQATHESVLSVDQSANSAPSVAA
                     KDESGEKVGRNDLCPCNSGKKYKNCGLLNTEEHKKNMAVKK"
     gene            13588..13827
                     /locus_tag="VHF05_02845"
     CDS             13588..13827
                     /locus_tag="VHF05_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79892.1"
                     /translation="MDTLEMSTKWIEAGFEFLFAEATQSHRTFRKFREEVYEKFDQVK
                     HRLDAVEKRLDAVEKRLSSLEEKVDEILQILKSKN"
     gene            13921..14667
                     /gene="atpB"
                     /locus_tag="VHF05_02850"
     CDS             13921..14667
                     /gene="atpB"
                     /locus_tag="VHF05_02850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVS79893.1"
                     /translation="MPHIEIGAEKVFSVFGLPVTNTLLLSWAVVLLIVIVIQIMSRKM
                     SLVPTGFQNAVEYVIEVLLGLMTSTFGSEHKAVKYLPYVATFFIMIVISNWAGVLPGV
                     GSIGLKVAAEGGGTFLLPFLRSPASDLNFTLALAIVSIVAINIFGVAAVGIGPHLKRF
                     FDFSSPINFFVGLLEIIGEFAKLISLAFRLFGNVFAGEVLLIIIGFLAPFIAPVPFLM
                     LELFVGVIQALIFATLTLVFLSIAVEKHHG"
     gene            14749..14979
                     /gene="atpE"
                     /locus_tag="VHF05_02855"
     CDS             14749..14979
                     /gene="atpE"
                     /locus_tag="VHF05_02855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HVS79894.1"
                     /translation="MENVDTLKNIAVAVAIFGGALGPGIAIGMIGAKGLDAIGRNPEA
                     SGKIQTAMIVAIAFAEAVAIYALVLGLIIKFV"
     gene            15110..15625
                     /gene="atpF"
                     /locus_tag="VHF05_02860"
     CDS             15110..15625
                     /gene="atpF"
                     /locus_tag="VHF05_02860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVS79895.1"
                     /translation="MSDLLHNFGIDWKLLIAQAVNFFILLYILKRYAYAPVVKMLNER
                     HRKIKKGLHDSAEAEKRLKDASEKGRDIVKAAEAKALDEIGRAEETAREREAELIEAA
                     HRKADSVLQSARKAAEGERERASEEMEGAAKDLVLLATAKVLGKMEPSERDAHLVGEA
                     LKELRVAAKHL"
     gene            15757..16143
                     /locus_tag="VHF05_02865"
     CDS             15757..16143
                     /locus_tag="VHF05_02865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HVS79896.1"
                     /translation="MRYSARQYAEALHKALEEKKGGERAEALTRFAALIRRNGDKSLL
                     ERILIQYERIDLEKSGTVKAEVETPEALAPHVRREIEKAIGKPVMLMEKSDPSLIAGI
                     RILLNDTLRIDASARTMIAGLFSPRK"
     gene            16227..17756
                     /gene="atpA"
                     /locus_tag="VHF05_02870"
     CDS             16227..17756
                     /gene="atpA"
                     /locus_tag="VHF05_02870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVS79897.1"
                     /translation="MSSPLIDNLKKEIEGAETGARRHEIGTVIEVGDGIARIFGLTNV
                     ALQEMVEFETSEGPVSGVAFNLEEDNVGAIILGDYAKVKEGDHVTHAGRILSVRVGEE
                     LIGRVIDPLGEPQDGKGPIFASESNAVYNPIESNAPSVVDRSPVNTPIHTGIKAIDSI
                     IPIGRGQRELIIGDRQVGKTSIAIDTIINQKNDTRHRTPICIYVAVGQKQSKVATVIA
                     TLEEKGAMEYTIVVSAPASSPASFRYIAPYSAAAIAEYFMNKGQDALVVYDDLSKHAV
                     AYREISLLLRRPPGREAYPGDIFYLHSRLLERAAKLNDKKGGGSFTALPIIETQLGDV
                     SAYIPTNVISITDGQIYLESDLFNQGVRPAVNAGLSVSRVGSSAQTKAMKKVAGRLRL
                     ELAQFRELAAFAQYSSELDEGTKRTINRGRVLTEVLKQNELEPMPFEKQIVLIYAATQ
                     GLFDAVPLDDVRKTYEEFLSFVDKLHADILASLKDSGELTDDTAAKLKAINEEFRQMK
                     S"
     gene            17762..18691
                     /gene="atpG"
                     /locus_tag="VHF05_02875"
     CDS             17762..18691
                     /gene="atpG"
                     /locus_tag="VHF05_02875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HVS79898.1"
                     /translation="MQSLRDLKRRIRSVKNIGQITKAMEVVSMTKMRRSQAFALDARP
                     YSIAAFEMFLNLLTMAPEQSGLLQARPDTKKLLAVVTSDKGLAGAFNENVLRMANRWI
                     EDRKKEGKEYALLVVGRKAKDAFERRGEPIVESFTGFGDYSTLEETEPVAKRIVGGFV
                     AGEWSGADIAFTHFRSTLKQEAVLRQVLPATKEALEEAVRAIVPESGKYHELAEEEIA
                     ETDGYSHEFIFEPSPKEILDELIPELLKIRIHHAILESNASEHSARMVAMKSASDNAR
                     ELSGELNLDYNKARQSGITAELAEVVAGQASVE"
     gene            18819..20243
                     /gene="atpD"
                     /locus_tag="VHF05_02880"
     CDS             18819..20243
                     /gene="atpD"
                     /locus_tag="VHF05_02880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVS79899.1"
                     /translation="MNHTHKTEKHTETKRRGKISQIIGTVVDVEFPEGHLPELFNALV
                     VHDGEREVVLETSRHLGFGVVRAIALSSTDGLKRGMEAADTGEPITVPVGPETLGKLF
                     NVFGKTIGDESDGSESEQHDFPRRAPIHRPAPSLTEQSTKVEIFETGIKVIDLLAPFV
                     RGGKVGLFGGAGVGKTVLLKELIRNVAEEAGGVSVFAGVGERTREGNDLLLEMKESGV
                     LDKAVLVFGQMNEVPGARLRTPLSALTQAEYFRDEEGKDVLLFIDNIFRFSQAGSEVS
                     ALLGRMPSAVGYQSTLASEMGALQERIASTKKGAITSVQAIYVPADDITDPAPAAVFA
                     HLDSTIVLSRALTDIGIYPAVDPLASASSALDPKIVGQEHYETARGVVRVLQRYAELQ
                     DIISLLGMEELSAEDRTTVARARKIQKFLSQPFFVGEVFIGIPGEYVKREDTVRSFKE
                     ILEGKHDDLPEDAFYMKGAIEKIK"
     gene            20351..20599
                     /locus_tag="VHF05_02885"
     CDS             20351..20599
                     /locus_tag="VHF05_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HVS79900.1"
                     /translation="MKARVYTLKGVLFEGEAKELIAETTTGEISILANHEPLVTVLKP
                     SELVVVKEDGERSAVRIEGGFLEVRPESEVIVLAKSAA"
     gene            20745..21143
                     /locus_tag="VHF05_02890"
     CDS             20745..21143
                     /locus_tag="VHF05_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79901.1"
                     /translation="MPALIKKHYGWEDSEDYDVAILAGGGGGLVSLDRDGRGQVVLND
                     FKVVTLGAHHGNEIILLNHQDCGMYAAWMEKKFKSGEEEEERLFHHEELKKEGARARK
                     EFPDPAIKIRLGYVFVNDKDEPDIEDVEFE"
     gene            21240..21662
                     /locus_tag="VHF05_02895"
     CDS             21240..21662
                     /locus_tag="VHF05_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HVS79902.1"
                     /translation="MKPKAFLFLLRVIMGWLFLYAGFSHITTPGWSAAGFLNGAKTFP
                     AFFHALTAPQILPMVNLANEWALVLLGVSLIAGLFVKWSAPLGAVLMLLYYLPGLSFP
                     HVASGYIVDEHIVYIFALLYMAAAGAGKAWGLDGKFHS"
     gene            21667..22266
                     /locus_tag="VHF05_02900"
     CDS             21667..22266
                     /locus_tag="VHF05_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79903.1"
                     /translation="MGKRILQSKKPKRPAHVRHPRHKMLRDLLIVAVSFAGAYYLAQF
                     GALNFLIASHGNNILGSFIAGALFTSTFTIAPAAVVLADIAHVASPYTVALWGAFGSM
                     LGDLVLFNFIKGNLAHDAEDILEHSPYKRFLSVFHLRFFRWLTPLVGALVIASPLPDE
                     IGLAMMGFSRMRTAIFLPISFAMSYLGILFICFLAQSVS"
     gene            22306..23337
                     /locus_tag="VHF05_02905"
     CDS             22306..23337
                     /locus_tag="VHF05_02905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HVS79904.1"
                     /translation="MNWRQYLNPLLIAVAVAAVFVLLGAFSTGEFADFANPAPEASST
                     GASATSSDSVTLVFAGDIMLSRAIGSLMAENGFDFPYARLDGTIRDADIAFANLETPV
                     STRGANVGSIYSFRSDPASLAGLKNAGFDVVSMANNHIWDYGRDAFLDTISNLDDAGL
                     KHAGGGATFADAHAPALVRAKGHTVALLAYTKLVPPFLSGNDSAPAVAYPDPTQIAAD
                     IAAAKKEGADIVIVSFHWGTEYSTTHDAAQEALAHAAIDAGALIVMGHHPHVAEEIER
                     YKGGLIMYSLGNFVFDQNFSADTSHGLLMRVVADGKGIESVEPLEVKFNASYQPVIAG
                     PWPQKANVL"
     gene            23368..23751
                     /locus_tag="VHF05_02910"
     CDS             23368..23751
                     /locus_tag="VHF05_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVS79905.1"
                     /translation="MKKKTIFITVAVIAVVAAIVTAVTIAPQKSGAYDQFAQCIKDSG
                     TTFYGAFWCPHCRDQKAEFGKSAKYLPYVECSTSDGQSQNATCNAAGIQGYPTWVFPD
                     HSTTTGVQTLQTLAEKTSCPLPSAQ"
     gene            23758..24312
                     /locus_tag="VHF05_02915"
     CDS             23758..24312
                     /locus_tag="VHF05_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HVS79906.1"
                     /translation="MTGFTEILTVILSSLAVIALLGALVAFYAAYARSSIGVRIREYI
                     GPKSLVLALLVAAGAAAGSLLYSYAAGFVPCDLCWYQRVFLFPQVVILAVALAKGDRG
                     AGRYGLALSIPGALIALYHTYIQLAGSGTLLPCAAAGAACSKVYFLEWGFITIPTMSL
                     IAFLALIAFYLVHGKASRSIKRLI"
     gene            24430..25464
                     /locus_tag="VHF05_02920"
     CDS             24430..25464
                     /locus_tag="VHF05_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HVS79907.1"
                     /translation="MKRFVVIGGGTGSHAVLRGLKRYPLDLTAVVSMFDSGGSTGMLR
                     DEFGILPPGDVRRCLVALAEENAPRGNGRRGGPNILRELFNYRFSEESSLKGHSIGNL
                     FLTALTQILGNEALAIREAGKVLNIKGRVLPVSLTNAHLHAELEDGTVIRGETNIDIP
                     KHDGSKRIRRAFLEPAAFAFEETEAAIRNADAIIIGPGDLYTSIIPNLLVKGIPEAIR
                     ESRATKIFVCNVMTKWGETNGFKASDFAREALFYSGLDAFDYVICSSTPLSLPTLRRY
                     SEEKSFPVICDPLLGNFARRVVREDVALASDIIRHDSEKLASVIIAQCHENAYLGFGR
                     NALERDRPVG"
     gene            25409..>25693
                     /locus_tag="VHF05_02925"
     CDS             25409..>25693
                     /locus_tag="VHF05_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79908.1"
                     /translation="MLISDLDGTLSSATGRSDSRPDSLRLLRPREGALELLTLPNFRN
                     FLVSTGEIHTETIKLGVLKFGHLFHGIYTVHSERDKRFVFEELFATHGRGL"
BASE COUNT         6187 a         6698 c         6980 g         5828 t
ORIGIN      
        1 gtacagaatg atgagtactg gggggaattt cccgaaaagg acctggacgt ggttcttgat
       61 aagtatagaa ggggagattc cgccgccgct agggcgcgca tcacagaact cggccgcgat
      121 gatttcgtgt tcggcttcgc gcgaagcgac tttttatacg ggcttgatat cggccccggc
      181 aagcgagtgc tcgacatcgg ctgcgggctc ggcgtccatt ctttcaatgc agcacggacg
      241 ggagcggaag tatatggatg cgacctgtcg agcaagcgcg ccgaattctg catgcttcgc
      301 gctaaaagcg acggagttga gaatgctcat tttttccatt ctgatatttt gaatcttccg
      361 ttcgcggcgg agtcgttcga cgcagtcgtc ctcaacggcg ttgtcgagtg gctcggtgaa
      421 atgaaccgca acaaggaccc gcgtcaagat cagatagagg ggctaaaatc cgttttgaga
      481 attttgaagc cgggcggcac gctttacatt ggcatcgaaa accggattgc cgcgacatat
      541 ctttttcatg ccacagatca caaccgactt aaatacacga cgttcatgcc gcgcttcctc
      601 gcggatttcg tgacgcgccg caagaaggga aaatcgtatc ggacatacac gtatgccaaa
      661 ggcggctatg aaaagttgct gcgcgactcc ggtttttcga accccgcgtg cttttacatc
      721 gcccatcccg gatataattt gccgcagtac ctcatcccgt tcggggatac ggccgcgctc
      781 tgttttgtcc tcaattccat gtcgatcgac aagggtatgc ggggagcatt tgtccgtgcc
      841 attgctgtat tgccgggtat gggaaaaatc acgaggaatt tcttttatag ctatgcaatg
      901 tatgtcaaaa aatgattgat caaattaccg acattgtgaa gaagaactgg gaaaaaatac
      961 ccgaagcggc gcgcgccgga gccgatccgg agcgaatttc tgtcacgaag ttccatcatg
     1021 gccgggcgct ttcgaagagc aaagtgctct ttttggttac atcaaaggac aaactcgttt
     1081 gcctggtgaa agccgcgcgc accgcggaag gcaatgaaaa actgcggcgc gagcgggatg
     1141 cacaggtgca attcggcagc acgcaactcc ttaaaacacc cgcggtttat ttcgacgggc
     1201 tgataaacgg cagcttcttt tatgcagaag aggttgtcga cggaaggccg ctctctcttt
     1261 ccgaagcgca atcgcgggaa tctgaaatta tcgcgatgat tgcttcatgg ccggcggaag
     1321 gcaccgtttc cacttcgaaa atcgccgaag tgttcggttc cgccatgaag gaggaagata
     1381 ctgatttcaa aaatttgcac aattcgctca caaatttgaa tgccaatttg aaaaagggaa
     1441 tgacgcacgg agattttacc cgtaagaaca ttttaaagca tgcgggtggg ttctatctgg
     1501 tcgattggga tcgcgcattt gagcgtccgt tctggcttct tgatgcagtg cactacctcg
     1561 tgcggtcgcg agatataaaa aattttgaag aatggaaaga aaagggaagt ccgattctca
     1621 tcaaggaagc attagttgat gaaccggccg ttagcgcgct ctatctcgca gaatcgcttc
     1681 ttgaactgct gtataagcgc tccccggaga gctatctccg cgccctggaa ggattttcac
     1741 gcctatgatc tccatcctct acattacgcg catgaatttc ttttcgaagc gcgcgcatgt
     1801 ctatacgacc gcgaatacat gcgcggcgat atcgaaagtt ggaggcgttt cgctcacact
     1861 ggtaagcacg gacgatagtt tgaaagggga gaaggaaagg agtgcgtttt ttgcggctca
     1921 aggcatcaat gaaccttttc caatcgtgtc gctcagttcg gctgcaaacc gctataagga
     1981 tagcgagaac gtgtggttga tcaggctctc ggtccttctt tcgaattttt cgcttgtccg
     2041 cgaagtgtgg gcgcgccgcc gcgctttcga cgtgctgtac gcgcgggacc atcaattgct
     2101 tctaccgatt gcctttgcca agtttgcgct tcgcaagcgg gtactattcg aaagccattt
     2161 tattctcgct aagcgcggaa gtcagtttgc ggtcgaattg gtggcgcgca tggcggatgg
     2221 tattgttgcc attacgcaaa atctccaagc ctattactcg cgcttcactc cgcgtacgat
     2281 cgtttcatat tgcgccgctt cggaaccgca acgtttttcc agcatttcgg aaagcgtgtc
     2341 ggagttgcga aaggcgctcg atttaccgca ggaggcgata attatctgct ataccggaaa
     2401 catggcatat acaggcaccg gacacagtta tggtatcgag gatataattc gcagtttgcc
     2461 gctgcttcct gaaaagtttg tctttgtggg tgtaggaagg aaagaaaatg aacgcctgct
     2521 gcctgagcaa attgcgtatg atcttggggt ggaaaatcgc gcgttattcc gcggatgggt
     2581 gacgcgcgat gaggtgacgc ggcacatcgt cgcagcggat attctcgttg ttccggcatc
     2641 gggcgcccag cccggcaatt ctcccactaa gatgttcgag tatcttgcgg cggggaagct
     2701 gatcgtcgcc gcgaataccg aagcgatcgc agaggtgctc cgcgacggag aaaacgcgtt
     2761 gatggttgat tataagaatc cgcaggcatg ggcggacgct ttcggaaaaa ttgttaatga
     2821 cgctggtcta tcaggaaagc ttgctgaaca cgcgcgtgcc gattctcaat tctattcttg
     2881 ggagaaaaga ggcgaaacaa tcgttgggtt cattagcgat actatccatg aataatatca
     2941 agcgaataat tttgcgaacc gccgccagga tgggcttgaa tgagcttgcg ggcagcactg
     3001 cttcacagtc ggctttctgc atcgggtatc acagcataag gagcaggcga aattcaaaag
     3061 aattatccgg agagctctat tggaacttgt ccatcgatga agaggagttt gatgcccaaa
     3121 taacatacct caaaaaacac gggcacacct tcgtaacagt cggcaacctt ctcaatgcgg
     3181 taaaaaaggg aatcaaaaaa ccgaccgcca tatacttcga tgacgggtat agggataatc
     3241 ttttgaatgc cttgccgata cttaaaaagc atgcggtgcc ggcgaccgtt tttgtcacga
     3301 ccggagcaat agatcgaacc tgcttgcttt ggacgctcag tctgcgcaat ctttacagga
     3361 aaatcggcaa agagaatgtt gaagaggaaa ttgccaaatt aaaggatttg ctggcgcatg
     3421 aagcggcaga gcgtgtggac gaagtgtata gggcacatgg ccttaagctc gattcggacg
     3481 atttcgacat gtttctttcg tgggaagaag tcggccgact ggcgcaagag ggtattgaga
     3541 tcggctcgca caccgttacg cacccgaatc tcacaaaagt ggatgcggcc aggatgaatt
     3601 cggaattggt cgaatcgaaa agccggatcg aggagaagat cggtgtgtcc gtgggctcgc
     3661 tgtcctatcc ttatggtagg gtaaacggcg aagtcgccga cgccgcgagg gcagcagggt
     3721 acaggtgtgc gatcggggga gtggaagggt ggaacagctt tcggacgatc cagtctcgtc
     3781 ggttcgagct tcgcaaaatc gcaccccggc ccggtggtga tatcgtcgat tttgcgacaa
     3841 gaatgtatct atttcctttg cggaaacgca tatctgaatt tttataaata tatgaacaaa
     3901 atactcgata attttaagag gaaattcttg cagtttcatc atgctaaaaa attctcatcg
     3961 cttggtgcaa actggcaagc aaatacggtg ctctttttct tttatctata ctctcgcttg
     4021 gctttaaggt ttgagctgac gtacttgcct caaacgaaaa ttaaattaag acaatttggt
     4081 aaggaattcg aatttatatt tactcacccg accgatatcc ccgtctttaa tcacatcttt
     4141 tttgaaggag aatatgaaat atcggattta aaaaaagaac cgttgataat tcttgatctt
     4201 ggaagtagtg taggaatttc agtattgtat tttcatctaa aatatccaaa agctgttatc
     4261 cacgcatttg aagccgatcc gaatactttc agaattttga aagaaaatac gaaaaatatt
     4321 gaaaatataa aagtaaataa tatttgttta tcagatagag atggagagaa ggaattattt
     4381 atatccgata caagcgtttc gtcttctgtt gttttaagat caaaaaatca acaaagcgtg
     4441 cttatgacgg caaagggcct tgattcaata attaaagaag tagtaagtac ccaagtaatc
     4501 gatttgatta agtttgatat tgagggagcg gaatttgagg tacttagcgc ttctaaagaa
     4561 aagagccaag ttgcttatat ggtgggagaa ctgcatcttg atcttattga gggcagtttg
     4621 gagaatttcc tggcccagtt ttctgatttt aaagtttcac tgaaaaaaaa ttcaagtcaa
     4681 agatatgctg tctatatgaa aaataaaaat cttatttgag ttgtacggat gttcttttag
     4741 tgaacgcatt ccaatatccc gcagaaataa gaaaggttgt gcctcgcgta atgacgcgcg
     4801 cccatactac tccccatagg ccgtagaaat aaacaaatcc gaaaataagg gtgatttgga
     4861 aaagggagga gtaaatcgtg gaagcataga gttctttctt tgccgactgg gcctggagcg
     4921 cgctggaagg aattacgctg acggtcacga tgagcgagag agaatatatt tgagaatagg
     4981 cgagggattc aagatattgg gggaagaata ttcgaaagat gaccggagca acggcaatgt
     5041 agaatgccac tccgagcacg acgacgagca tgaagagcaa tatttttttc cagattgtcg
     5101 cattgatatc atgcgcatcg cgttcgctga acttgggcag ggcgagattg cccacgttct
     5161 tgatgacacc cttgagctgc tccggtatcg cgatagcgaa cgcataaatg gcgaggcttg
     5221 cagcgccgag ataatggaag atcaggatct ggtcgatgtt gtttgcgacc gcactgaaga
     5281 tgttcatgag cgacagatgt ttcccgtagc caatgcttgc gctatccatg tcatcgtttt
     5341 tcttataagc gcgcgcgaca tacgcataga tcgcgagatt cgatgctgta tttgagatgt
     5401 agtacgccag aatgaggatg acgaaattct gggtgagtat catgcatcca atgagaatgg
     5461 ccgcgggaac ggcgacgctc aatccgtcga ggagagcgct tgctttgaag gctttcttcc
     5521 cggcgaggaa tgaatcatag aaattcgcac tggcgaaaac gggagcgaga aatcccgcga
     5581 tcaagaccga aaccgccaat gcgtgattcc cttgggcgta ataatacgcc gccacgacga
     5641 ccattgcgac ggtgaagaac gtgctccatt tgagattgag cgcgaatgct ttttgcagga
     5701 aaccgtcctg gttgcgcgcc gccgcctgga caatgacggt cccgagaccc gagagggtga
     5761 gtgacgaaag gagagtggcg atcgatagca cgaatttgta gtttccgtac gtgtctttcg
     5821 acaggaaatg ggcgaacgcg agcgccaggc agaatgaagt gagcgcggcg atagtttgtc
     5881 ccacggtcat ccagaacgtg cccgaaaaca catatttgga atcaaaaccg aggatcagcc
     5941 cgattcttac gaccatattg ttgagtttcg tccgtatgga attcacggct ccattctaga
     6001 ccaggtgcct tcctttagct agcaggagag gggtttcggt ggtatattca aaatcatgaa
     6061 attcaaaacc atactccgct atatcgtttt ggcaggcatt ttcgtcgttc cgttccttcc
     6121 gttttttgtg gcgaattcga tgtatttccc gtttatttcg ggcaaaaact tcgcgttcag
     6181 gatcatcatc gaaatcatct tcgcgtgctg gctcatcctc gcgctctccg ataagtccta
     6241 cgccccgaag aagtcgccga tcatgtgggc gtactcggcg ttcatggcta tcgtcattct
     6301 cgccgatata ttcggtgtga atccgtaccg cagcttctgg tcgaactacg agcgcatgga
     6361 aggccttatc gcgcatctcc acttcttcgc atacttcctc gttctcggct cgacgctctc
     6421 gggccagaag ctctgggatg cgttctggaa gacgtcgatc ggcgcgagcc tcattatggt
     6481 ggtctacggc tttgcccagc tcgggggcgt tgcggcgatc caccagggag gcgtgcgtct
     6541 cgacggcacc ttcggcaacg cggcatactt ggccgtctac atgcttttca atattttcct
     6601 ttccgcgtac tatctcgcgc atacctggaa aaagcctgcc gagcgctgga tctacatggt
     6661 cacgattccg ctccagttca ttattctttt ctataccgcg acccgcggct cgctcctcgg
     6721 cctcttgggc ggcgtattcc tctcggcggt gcttctcgtg atcgccgagc gcaagcggaa
     6781 aaagctcagg aatatctcca tcggcatcgg cgccgcgctc atcgtgctgg ttctggtttt
     6841 tatcgcaatg cggaacgtgc cgttcatcaa gaacaacgaa gtgctcggac gcttcgcctc
     6901 gatctcgctt catgacaaga ctaccgagtc gcgcttcatg atctggaaca tggcctggca
     6961 gggcgtcctc gagaggccgg ttctcggctg ggggcaggag aatttcaacc tggtcttcaa
     7021 caagttctac aatccgaaga tgtacgacca ggagcagtgg ttcgaccgca cgcacaatat
     7081 cgttttcgac tggctcattg atgccggctt cctcggcgct atcggatact tcgcgctttt
     7141 cggcctcatc atctacggca tctggaaaac gaagcagttc agctctcttg aaaagagcat
     7201 tctcgtcggc atgatggccg ggtacttcgt ccacaacttc ttcgtcttcg acaatttggt
     7261 gagctacatt atctacttct cgatcgctgg ctggatctat tcgctccaga cggaggagaa
     7321 gcgagccgcg gcggcaggga agccgatgat gaacatggaa gtcgtgaatt acggcctcgc
     7381 tccggtcgcg gccgtcgcgc tcatcctctg cgtctacttc ctcaacaccc gtccgatcca
     7441 ggcgaacgaa acgcttatcc aggcgcttca gaacgaaaag gatccgcaga catcgctcat
     7501 gtattatcag aaggcgatcg gctacggcac cttcggcagc ggcgaagcgc gcgagcggct
     7561 cgtgatcgac gcgccgaaca tggcggcgaa cgcttccgcg gatgtcaaaa gccaagtcct
     7621 ctcctatgcc gcttcgcaga tggaggagca gatcaaggcc gctccgaacg atgcacgcta
     7681 tgagctcttc ctcgcttcgt tctacgacgg catcggagac tacgatgacg ctcttgtgca
     7741 cgtcaatcgc gccctcgaag agtcgcccaa gaagcaggcg atactcatcg agaaaggcgt
     7801 cgcctacatc gggaagaagg attatcagga tgccctagcc gccttcaagg aagcgtacga
     7861 tcttgagccg gattacaccg acgcgaagac gatctatgcg gcgggggcgg cgtatgcggg
     7921 agacaaggat ctcgcgatgt cgcttctctc gggtttgagc gaccaggatc ttcttacgga
     7981 cgaccgcgcc ctcggcgcat atgcccagct cggcgattac gacaaggtgc ttcagatcta
     8041 tcagctgcgg cttgccaatc atccagagaa ggatgatgcc cgcgcccaga tcgagctctc
     8101 gatcgcggcg acctacatgc agctcggccg caagaccgat gcgatcgctg cgctccagca
     8161 gatcgcttcg gagagcccgc agtacgcttc ccagatacag cagtatatcg acgacatcaa
     8221 ggcggggaag aatccagtgc agcaatagaa gaaattgacc taattgcact aattatccta
     8281 atttctaaaa aagtcccaat ggccagtatc gaaacccgaa aaaacggtat attcgatttg
     8341 gaagaacgga ctgcgaaatt cggagaagcg gtcattgcgt tttgcaggaa attgccgaag
     8401 gacgaggtaa ctcgtccttt aatcgttcag cttgtgaaat gcggcacaag cgtcggggcg
     8461 aattattgcg aagctgatga tgcggagagt ggcaaggatt tcaagcataa gatcggtatt
     8521 tgcaagaaag aatcaaggga agcgaaacac tttctcagaa tgagtgccgt agcagtacct
     8581 gaatacaaaa acgaggccag aaaattatgg ctcgaggcaa aagaactgaa tttgatattt
     8641 aacagcattt acaaaaaagt taaataacat tcggtgatta gtattcaggt attatttagg
     8701 tcaattagga taattaggag taattagaaa ttctagatgc acttgcgcgg atttcccgcc
     8761 ttgctatact agacatgtga gcgctaacgt aagttggcac ccaaaaatga atgtctgcac
     8821 aaggcccccc accacggggg ttttgtgttt ttattttgac aaattacata tatattgtta
     8881 catttccttc ggaattattt ccttgaaagg ataactgagt ggaatcagaa aattatctct
     8941 acactttcca cccggggaga agaagtatac ttgcggaaat atattttccc caaaggattg
     9001 ccgcccaggg aaccatctac gatacgcttg aggcaggatt aagaccccaa gaagttcgta
     9061 attaccttgt attaaacgtt caacgcataa tgcgtgaaat ggcagcttat ccgcactggt
     9121 tcgatccata ccgctacgga ggtggcgaac cacttgataa cccaaccatc gaagatgccc
     9181 aaaagcgcat gaaaatgtgc aatcaggtgt ttttcggctg gtcgaaatat gaggtcgacg
     9241 gggtattttt gaagcgtgat ggtgtgacga tcgacgagga gcgtacgcaa atcattcggc
     9301 tcatgtttaa gtttgaagat gagatgactg aaaaatggca tgggagaagg ggtacccgga
     9361 tgtgtaccgc tctatcctct cgttcattct tgccgaatat gcccatacgg acaattatcg
     9421 cgtttgggaa gaaattgaat tccaaaggct tttgcaacgc caatctttct ggaatgagaa
     9481 aaagacggaa ttcgctcggg agctttacat catacttgct cccaagatgg ccaaatgggt
     9541 cgtcgattgc ggcttattta ttttcggcta ccttgtccgg gagttctgga acaagatcgt
     9601 agatctaaac aagaagcgcg gcgcgcctct cgaagacgaa atctgggtgg cgagcatctt
     9661 tcatctctcc ataaatgtca tggctcctat gaaatacatg ttgccgtgat atagtgaaaa
     9721 acagaaagtc caaacaagga gttaaacaat gtcagaagta gatagacccc aatattcgga
     9781 agaagttctt caagaactca aaatgctttg cgaaagcttc ggttccggtg aagaggcgcg
     9841 caatgatccg aatttcgtac taacctcgtt actcaagaga ctgaatgagc gaaaggacag
     9901 cataagcgca atgacacgaa cagaccttga atggtcgatt acccctcaat ctttgaagtt
     9961 cattcgttcg caggcggctg aacaaaagag cgctgcttag aataaatctt aagtttacat
    10021 tgtccggcac atggactgtg ccggactttc ttttatatat gctattgcct tataatagga
    10081 gggtgagcgg ttgagggctt gtaccgggcc atgaccagcg gttcgcactc acattataga
    10141 catttatttc aattttggag ccagcttccg ctggtcatga gccggcagaa gtccaagacg
    10201 aaaataaaaa gcacggcagc cgttcgttcg cgagcggctg ccgtgctttt tattcctccg
    10261 cgttttttta tcgaaaaaat tttcgaatga atttcatttc gggaattttt tttgaaaaac
    10321 tgatgagcaa gaaatttttt cagaatattt tcagaaaaaa atttttcgag ttatccacaa
    10381 tttgcgcgca aaaaatattg tgtgaaatat ttttccgtat gataatttaa tcaaggtcgt
    10441 aacggcgcct gaaaaaattt tgaaagaaat ttttcgaaac gccgaaaact tataagtaat
    10501 gacgcacgat gtttctctcg ctcggatact gtagagccgt gagagctgat gcgaaatact
    10561 atggcaacta agaaagcagc aaagaagaag acgaagaagg ctgcaaagaa gtccaccaag
    10621 aaggcttcga agaagaagac cgcgaagagg aggaagtaag cgcgtcttgc agtctgatat
    10681 gaaatctccc cggcaacggg gagattttgt ttttccctga acggcagttt ccgatctctc
    10741 tttcagccct ttatcttgac cgtattttta atataggttt tatgctacaa tagggcggct
    10801 attccgaaaa ctcagggaaa aagcagcaaa atacagctaa aactatggat tttttaaaga
    10861 atcttttcgg cgacgacagc tcgcgcgaag tcggccggat catgaaaata gtggagaagg
    10921 tgaatgcgtt cgagccagaa atttcgaagc tttccgacga ggagcttaga gggaagacgg
    10981 ctgaattcaa agatcggctt gcacaaggcg ctactctcga cgacattctt cccgaggcgt
    11041 tcgcggtcgt ccgcgaagcg tcccaacgga cgctcaagca gcgccactac gacgttcagc
    11101 ttgtcggcgg cgtcgtcctc cacgaaggca gtatcgccga gatgcgcacc ggagaaggaa
    11161 agacgctcgt cgcgacgctt cccacgtatc tcaacgccct taccggaaag ggcgtgcatg
    11221 tcgttaccgt aaacgactac ctctcccgcc gcgacgcggt gtgggtcggc cagattcccg
    11281 cattcctcgg gcttacggtc ggcgtcatta ccaccgacgg ctcatatata tacgatccgg
    11341 cgcacaagga tctcgatcag gagcgcgacg aagtcggcgc tttcaaggtc gtgcatgaat
    11401 tcctccgccc ggtttcccgc cgcgaggcgt acgaggccga catcacctac ggcaccaaca
    11461 acgaattcgg cttcgactac ctccgcgaca acctcgcgta ctctaaggat caggtccgcc
    11521 agcgcggcta ccactacgcc gttgtggacg agatcgactc gattctcatc gacgagtcga
    11581 ggacgccgct cattatttcc atgccggccc aggaatccga gcgcctctac gagacgtttg
    11641 ccgatattgt ttcgaagctt aaggtcgacg aggattatac cgtcgacgag aaactgcacg
    11701 ccatccagat gaccgacgag ggaatcacca aggccgagaa gatgctcggc gtggagaaca
    11761 tctataccga ccgcggcatc aagtacgtcc accatctcga aacggccgtg cgcgccaaag
    11821 ccctctacac ccgcgacaag gaatacgtgg tgcgcgatgg ggaagtgatc atcgtcgata
    11881 cgttcaccgg ccgcctccag cccggcaggc gctggtcgga aggcttgcac caggcgatcg
    11941 aagccaagga aggagtgcat gtccagaagg agtcccgcac cgccgcatcg atcacgttcc
    12001 agaactactt ccgtttctac gaaaagctct cgggcatgac cggtaccgcg aagacctccg
    12061 cggaggaatt ctacaaagtc tacgagcttc ccgtcgtaac catcccgacc aacaaaccga
    12121 ccatcaggaa ggacgacaac gatctcatct tccagaccga gaatggaaag ttccgcgctc
    12181 ttgcccgcaa ggtcaaggag ctcaacgaaa agggccagcc ggtcctcatc ggaacggttt
    12241 ccatcgaaaa gaacgaactg ctctccgagt acctcaataa ggaaggcatc aagcacacgg
    12301 tcttgaacgc caagaaccac gagcgcgaag gcgaaatcat cgcccaggcg ggacagaagg
    12361 ggggcgtgac ggttgccacc aacatggcgg gccgcggcgt cgacatcaaa ttgggcggaa
    12421 gcccgtcgac cgacgaggaa tacgaagagg tgaagaacct cggcggcctc tacgtcatcg
    12481 gcacggagcg gcatgaagct cgccgcatcg acaaccagct ccgcggacgc gccggccgcc
    12541 agggagatcc gggcgagacg cagttctatg tctccctcga ggacagcctt atgcgcgtct
    12601 tcggttccga caggatcaag ggcatgatgg gccgcttcgg cattcccgaa gacgagccga
    12661 tcgaaaacag gcttatcaat cgctcgctcg agagcgcgca ggagaagatc gaaggattcc
    12721 acttcgatgc ccgcaagcac gtgcttgaat tcgacgacgt gctgaactat cagcgcaaga
    12781 tcgtctacga gcgccgcagg gcgattctct tcgccgacaa ggcggagctc accgcgcaca
    12841 tccagtcgga agtgttcgat ccggctcccg acgaaatccg ccccggcctc gaggccaagc
    12901 gccgccagct cggcgaggat ctcttcctcg aggcgatgcg cgccgtcatt ctccagacga
    12961 ttgacatgta ctggatcgac catctcgaga ccatggatta cctgcgcaac agcgtcaatc
    13021 tccgcgctta cggccagcgc gatccgctcg tcgaatacaa gcgcgaaggc ctgaggcttt
    13081 tcaaggaaat gcaggatgcg atccgcgacc agacctgggc tgtcattccg aacatcggcg
    13141 gccccgttcc gaccgaagag gagaagcctc tccaggcgac tcacgaatcg gttctgtctg
    13201 tcgatcagtc ggcgaactcc gctccttctg tcgcggcaaa ggacgaaagc ggcgagaaag
    13261 tcggccgcaa tgacctatgt ccgtgtaatt cgggaaagaa atataagaat tgcgggctct
    13321 tgaataccga ggagcacaag aagaacatgg cggtcaaaaa ataacctcga ggctattttt
    13381 ttgatatttc gaaatatcga aatttcgaaa ttcttttcat ttttattcaa ctcagatctt
    13441 gatgagctaa tttaattcac aaaatatgac tgcgggcaga tgtttaacga cataacgcct
    13501 gaatttgcat tatgttcctg cacatgatag tttgagggcg gaagttgcca taaggagggc
    13561 ataatgccag ggacaattga agagagagtg gacacactcg aaatgtccac aaagtggatt
    13621 gaggcaggat tcgagttttt gtttgcggaa gcaacgcaat cacacaggac attccggaaa
    13681 ttcagagaag aggtttacga gaaattcgat caagtcaaac atcgcctcga cgcggtggaa
    13741 aagcgcctcg acgcggtgga aaagcgcctc agttctttgg aagagaaggt tgacgaaatc
    13801 cttcaaattc tcaaaagcaa aaattaagat tttaaaaaga tagcatctct gcccgcaact
    13861 cgttttgcgg gcttcttttt cgaaatttcg acatttcaaa atatttagta tcataatggc
    13921 atgccgcata tagagattgg cgcggagaag gtgttttcgg tgttcggcct ccccgtaacg
    13981 aacacgcttc ttttgagctg ggcggtcgtg ctcctcatcg tcattgtcat ccaaatcatg
    14041 tcgcggaaga tgagcctcgt tccgacgggc tttcagaacg ccgtcgagta cgtgatcgaa
    14101 gtcctgctcg gcctcatgac ctctaccttc ggctcagagc ataaggccgt gaagtatctc
    14161 ccgtatgtcg cgacattctt catcatgatc gtcatctcga actgggcggg cgtgcttccc
    14221 ggcgtcggct cgattggctt gaaggtcgcg gcggagggcg gcgggacgtt tctcctgccg
    14281 ttcctgcgtt cgcccgcgag cgatctcaat ttcacgctcg cgctcgcgat cgtctccatc
    14341 gtcgccatca atatcttcgg cgtcgccgcg gtggggatcg gcccccacct caagcgcttc
    14401 ttcgacttct cgagcccgat caactttttc gtcggccttc ttgagatcat cggagaattc
    14461 gccaagctca tctcgctcgc gttccggctt tttgggaacg tcttcgccgg ggaagtgctc
    14521 ctcattatca tcggcttcct ggcgcccttc atagcgccgg tgccgttcct catgctggag
    14581 ctttttgtcg gggtcatcca ggcgctcatc ttcgcgacgc tcaccctggt gtttctgagc
    14641 atcgccgtgg agaagcatca tggttgaccg gcgagcatat atgatagaat ccaggcatcg
    14701 gaaacgggcg gtaggtataa ataattatca ataggaaagg aaaatcttat ggaaaacgta
    14761 gacactttga agaatattgc ggtcgcggtg gcgatattcg gaggcgcatt gggtccggga
    14821 atcgccatcg gcatgatcgg cgccaagggc ctcgacgcca tcggccgcaa tccggaagcg
    14881 tcgggcaaga tccagacggc aatgatcgtg gcgatcgcgt tcgccgaggc ggtggcgatt
    14941 tatgcgctgg tgctcggcct tattatcaaa ttcgtttaaa gagaaggcaa aattcaaaat
    15001 gcaaaactca aaattgcaat tcaaaattgg aaaaattgaa tgcaatactg gggtttttga
    15061 ttttgattta aaattttgct ttttgacttt tggtttttga atttccttca tgtctgacct
    15121 gcttcacaac ttcggcatcg attggaagct gcttatcgcc caagcggtga acttcttcat
    15181 cctgctctat atcctgaagc ggtacgccta cgcgccggtc gtgaagatgc tcaacgaacg
    15241 ccatcgcaag atcaaaaaag gccttcatga cagcgccgaa gccgagaaga ggttgaagga
    15301 cgcttccgag aagggcaggg atatcgtaaa ggccgcggaa gcaaaagcgc tcgatgagat
    15361 cgggcgcgcc gaagagaccg ctcgggagcg ggaagcggag ctcatcgaag cggcgcatcg
    15421 gaaggccgac agtgtcctcc agtcggcgcg gaaggcggcg gaaggggagc gagaacgggc
    15481 ttcggaggaa atggaaggcg cagcgaagga tctcgtcctt cttgcgactg ccaaagttct
    15541 tgggaaaatg gagccgtcgg agcgcgacgc ccatttggtg ggagaggccc tgaaggagct
    15601 tcgcgtagcg gcaaaacatc tatgaccctg ttgtagaaaa tagcatttaa aaaactgcaa
    15661 ttcttagtaa ctttgattca tgtgcataga tacattacaa attaatcttg gaaaaatatt
    15721 gctccgaagg agcgatgcca tttttcacaa cagggtatga gatacagcgc gcgacaatat
    15781 gccgaggcgc tccacaaggc gctcgaggaa aagaagggcg gggaaagggc cgaagcgctt
    15841 acgcgcttcg cggcgcttat ccgccgcaac ggcgacaaga gcctcttgga gcgcattctc
    15901 attcaatacg agcgcatcga cctcgagaag agtggcacgg tgaaagccga agtcgaaact
    15961 cccgaggcgc ttgcgcccca tgtgaggcga gagatcgaga aggcgatcgg caagcccgtg
    16021 atgctcatgg aaaagagcga tccttcgctc attgcgggaa ttcggatact cctcaatgac
    16081 acgttgcgca tcgatgcgtc cgcacgaacc atgattgccg gactattcag tccaagaaaa
    16141 taattttcaa ttatcaattt tcaatcaatt ctcaatgaaa caattttcaa gcttcagata
    16201 ttgaaaatta aaaattcgca cgcaccatgt cctcaccgct tatagacaat cttaaaaaag
    16261 aaatcgaagg agccgaaacg ggcgcccgcc gccatgaaat cggcaccgtc atcgaagtgg
    16321 gcgacggcat tgcgcgcatc ttcggcctca ccaacgtagc gctccaggaa atggtcgagt
    16381 tcgaaacctc cgaaggcccc gtttcgggcg tcgcattcaa tctcgaggag gacaatgtcg
    16441 gcgcgatcat cctgggcgac tacgcgaagg tgaaggaagg cgaccatgtc acgcatgcgg
    16501 ggcgcatcct gtcagtgagg gtgggagaag agcttatcgg ccgcgtcatc gatcccttgg
    16561 gcgagccgca ggatggaaag ggcccgatct tcgcttccga aagcaatgca gtatacaacc
    16621 cgatagagag caacgccccg tccgtggtcg acagaagccc ggtgaatact ccgatccaca
    16681 ccggcatcaa ggcgatcgat tcgatcatcc cgatcggccg cggccagcgc gagctcatca
    16741 tcggcgaccg ccaggtcggc aagacgtcga tcgcgatcga cacgatcata aaccagaaga
    16801 acgacacgag gcaccgcacg cctatctgca tttatgtcgc ggtgggtcag aagcagtcga
    16861 aggtcgccac cgtcatcgcg acgctcgaag agaagggcgc gatggagtac accatcgtcg
    16921 tttcggcgcc cgcttcctcc ccggcgtcgt tccgctatat cgcgccctac agcgccgccg
    16981 cgatcgccga gtattttatg aacaagggcc aggacgcgct cgtggtttac gacgaccttt
    17041 ccaagcacgc ggtcgcgtac cgcgagatct cgctcctctt gcgccgccct cccggccgcg
    17101 aagcgtatcc gggagacatc ttctatctcc attcgaggct tctcgagcgt gcggcgaaac
    17161 tgaacgacaa aaagggcggc ggcagcttta cggcgcttcc aatcatcgag acccagctgg
    17221 gcgacgtgtc ggcctacatt ccgacgaacg tcatttcgat taccgacggg cagatctatc
    17281 tcgagtcgga cctcttcaac cagggtgtcc gtcccgcggt gaacgccggt ctctcggttt
    17341 cgcgcgtcgg ctcgtccgcc cagacgaagg ccatgaagaa ggtggcaggc cgcctgcgcc
    17401 ttgagctcgc ccaattccgc gagcttgccg cgttcgccca gtattcctcc gaactcgacg
    17461 agggaacgaa gcgcaccatc aatcgcggca gggttctcac cgaagttctg aagcagaacg
    17521 agctcgagcc gatgccgttc gagaagcaga tcgtgctcat ttatgcggcg acgcagggac
    17581 tcttcgacgc cgtgccgctc gacgatgtgc ggaagacata cgaggagttc ctctcgttcg
    17641 tcgataagct ccatgccgac attctcgcgt cgctcaagga ttcgggggag ctcaccgatg
    17701 acaccgcggc gaagctcaag gcgatcaacg aggaattcag gcagatgaaa tcataaagga
    17761 tatgcagtca ctgcgagatc taaaaagacg aatacggtca gtcaagaata tcggccagat
    17821 caccaaggcg atggaagtcg tgtccatgac aaagatgcgc aggagccagg cattcgcgct
    17881 tgatgcgcgg ccgtattcca tcgccgcatt cgagatgttc ctgaaccttc tcacgatggc
    17941 gcccgaacag agcggacttc tccaggctcg ccctgacacg aagaagcttc ttgcggtggt
    18001 gacgtcggac aagggccttg ccggcgcgtt caacgagaac gttttgcgca tggcgaatcg
    18061 ttggatcgaa gatcggaaga aagaaggcaa ggagtacgcg cttctcgtcg tcggcaggaa
    18121 ggccaaggac gcgttcgaac ggcgcggcga gccgatcgtc gaaagcttca ccggcttcgg
    18181 cgactactcg accctcgagg agacggagcc cgtcgcgaag cggatcgtcg gagggttcgt
    18241 cgcgggcgaa tggagcggcg ccgacattgc cttcacccat ttccgctcga ccctgaagca
    18301 ggaagccgtg cttcggcagg tcctgccggc gacgaaggag gcgctcgagg aggccgttcg
    18361 cgcgatcgtt cccgaatcgg gcaagtacca tgaacttgcc gaggaggaaa tcgccgagac
    18421 tgacggctat tcgcacgaat tcatcttcga gccgagcccc aaggagattc tcgacgagct
    18481 cattccggag cttttgaaga tccgcatcca tcacgccatc cttgaatcga acgcgtccga
    18541 gcactcggcg cggatggtcg cgatgaaaag cgcttctgac aatgcgcggg agcttagcgg
    18601 cgagctcaac ctcgactaca acaaggcgag gcagtcgggc atcaccgcgg agctcgccga
    18661 agtcgtcgcg gggcaggcaa gcgtcgaata aattcaaaag tcaaaatcca aaatgcaaaa
    18721 gtttaaatta aaatcagaaa gtatccggaa tgtagccgaa ttaccgcttt tgaattgcaa
    18781 ttttgacttt tgacctttga attttgaatt aaatttttat gaaccacact cacaaaaccg
    18841 aaaagcatac ggaaacgaaa aggaggggca agatatctca aatcatcggc acggtcgtcg
    18901 atgtcgagtt tccggaaggg catctgccgg agcttttcaa tgcgctcgtc gtgcacgacg
    18961 gagagcggga agtagtgctc gaaacgtcgc gccacctcgg attcggcgta gtacgcgcca
    19021 tcgcgctctc ctcgaccgac gggctgaagc gcggcatgga ggcggcagat acgggcgagc
    19081 cgattaccgt gccggtgggt cctgaaacgc tcggcaagct tttcaacgta ttcggcaaga
    19141 cgatcgggga cgaatcggat ggatcggaat ctgaacagca tgattttccg cgccgcgcgc
    19201 cgatccacag gccggctccg tccctcacgg agcagtcgac caaagtcgag attttcgaga
    19261 ccggcatcaa agtgatcgat cttctcgcgc cgttcgttcg cggcggcaaa gtcggcctct
    19321 tcggcggcgc gggcgtcggc aagaccgtgc ttctcaaaga gctcatccgc aacgttgcgg
    19381 aggaagccgg aggcgtttcc gtttttgcgg gcgtcggcga gcgcacccgc gaaggcaacg
    19441 acttgcttct tgaaatgaag gaatccggcg ttctcgacaa ggccgtgctc gtcttcggcc
    19501 agatgaacga agttcccggc gcccgccttc gtacgccgct tagcgcctta acgcaggcgg
    19561 aatatttccg cgacgaggaa ggcaaggacg tgcttctatt catcgacaat attttccgct
    19621 tctcgcaggc gggcagcgaa gtctcggcgc tcctgggccg catgccgtcc gccgtgggct
    19681 accagtcaac gcttgcctcc gaaatgggcg cgcttcagga gcgcatcgcc tcgaccaaaa
    19741 aaggcgcaat cacttcggtg caggcgatct acgttccggc cgacgatatc accgacccgg
    19801 cgccggccgc ggtcttcgcg cacctcgact cgaccatcgt gctctcccgc gccctgaccg
    19861 acatcggcat ctaccccgcc gtcgatccgc ttgcctccgc ctcgagcgcc ttggatccga
    19921 aaatcgtcgg gcaggagcat tatgagaccg cccgcggcgt agtccgcgtg ctccagcgct
    19981 acgcggagct tcaggacatc atcagccttc tcggcatgga agagctttcc gccgaagaca
    20041 ggacgacggt cgcccgcgcc cgcaagatcc agaaatttct ctcccagccg ttcttcgtcg
    20101 gcgaggtctt catcggcatt ccgggagaat acgtgaagcg cgaggacacg gtgcggagct
    20161 tcaaggagat cctcgaaggc aagcatgacg acttgcccga ggacgccttc tacatgaaag
    20221 gggcgattga aaaaatcaaa taaaatttgc aattctcaat tttcaatgaa tttttaattt
    20281 tcaattttac aaacgtttga aaattaacac attgataatt gattgaaaat taaaaattta
    20341 aaattttaaa atgaaggctc gcgtctacac attaaaagga gtgcttttcg aaggcgaggc
    20401 gaaggagctc attgccgaaa cgactactgg cgaaatcagc attctcgcga atcacgagcc
    20461 gctcgtgacg gtcttgaagc cttccgagct tgtcgtagtg aaggaggacg gggagaggag
    20521 cgctgtgcgc atcgaaggcg gctttctcga ggtgcggccc gaaagcgagg tgatcgtgct
    20581 tgcgaaaagc gccgcctgaa atattgcatt taaaataatt tcgtggtata attttaaaag
    20641 aaggaaacaa tatgttgctt aaagaaaaat cgaagatact gtccgttcat aagccagtaa
    20701 aaggaggcgt atgtttctgt acggagccga gattccggga aaaaatgccc gctctcatca
    20761 aaaaacatta tggatgggaa gatagcgaag attatgatgt cgccattctt gctggcggag
    20821 gaggaggact ggtgtctctc gatcgggacg gccgcgggca agtggtgctg aatgatttca
    20881 aggttgttac gttaggcgct caccacggca atgaaatcat tcttctcaat catcaggatt
    20941 gcggaatgta tgccgcatgg atggaaaaga aattcaaatc cggagaagaa gaggaagaac
    21001 gcttgtttca tcatgaggaa ttgaagaaag aaggagcgag ggcgagaaag gaatttcctg
    21061 atcctgcaat caagattcgg cttgggtatg tcttcgtcaa cgataaagat gagcctgata
    21121 tcgaggacgt cgaattcgaa taaggttcag tactgtctta aagaggcgat tcatcgcctc
    21181 tttttttgtt ttccacttct gcggggcttc gggatgccgt atgatgaaag agggaatcta
    21241 tgaaaccgaa agcatttctc tttttgttgc gggtgataat gggctggctt tttctctacg
    21301 ccggcttttc gcatattacg actccgggct ggtcggccgc cggattcttg aacggcgcca
    21361 agacgttccc cgcattcttc catgcgctca ctgcgccgca gatattgcct atggttaacc
    21421 tggcaaacga atgggcgctc gtgcttctcg gcgtctcgct catcgccggg cttttcgtga
    21481 aatggagcgc gccgttggga gccgttctca tgcttctgta ctacttgccg ggactttcat
    21541 tcccgcatgt tgcatcgggc tatatcgtcg atgagcatat cgtgtatatc ttcgcgctgc
    21601 tctatatggc ggccgccggg gcggggaagg cgtggggcct cgacgggaaa tttcattcat
    21661 aaataaatgg gaaagagaat tctgcaatcg aaaaaaccga agcgtccggc tcatgtgcgc
    21721 catccgagac acaagatgct tcgcgatctt cttatcgttg cggtgagttt cgccggagcg
    21781 tattacctcg cgcagttcgg agcgctcaat ttcctcatcg cctcgcatgg caataacatc
    21841 ctcggaagct tcattgccgg cgcgctcttc acgtcgacct tcaccatcgc gccggccgcg
    21901 gtcgttctcg ccgacatcgc ccatgtcgcc tcgccctaca cggtggcgtt gtggggcgcc
    21961 ttcggttcga tgctcggcga cttggtgctg ttcaatttca taaaaggcaa tctcgcccat
    22021 gacgcggagg atatcctcga gcattcgcca tataagcgct ttctctccgt ttttcatctg
    22081 cgcttcttcc gatggctgac gccgcttgtc ggcgcgctcg tcattgcctc gcccttgccc
    22141 gacgagatcg gtctcgccat gatgggcttc tcgcgcatgc gcaccgccat tttcctgccg
    22201 atctccttcg ccatgagtta cctgggcatc ctcttcatct gcttcctcgc ccagtcggtt
    22261 tcgtagctcc tatcgcggaa cggcgttcca tactatgctt agagaatgaa ctggcgccaa
    22321 tacctgaacc cgcttttgat cgccgtcgca gtcgcggcgg ttttcgtcct gctcggcgcc
    22381 ttctcgaccg gcgaattcgc cgatttcgcg aatcccgctc ccgaggcttc ttcgaccggc
    22441 gcgagcgcga catcgagcga ttccgtcacg ctcgtcttcg cgggcgacat catgctttcc
    22501 cgcgccatcg gctcgctcat ggcggagaac ggcttcgatt tcccgtacgc gcgcctcgac
    22561 ggcacgatac gggacgccga tatcgcgttt gccaaccttg aaacgcccgt gtcgacgaga
    22621 ggcgcgaatg tcggaagcat ctattcgttc cgctccgatc ctgcctcgct cgccggtctg
    22681 aagaatgcgg gcttcgacgt cgtctcgatg gcgaacaacc acatctggga ttacggccgc
    22741 gacgcgttcc tcgatacgat ctcgaatctc gacgacgcgg gattgaagca tgccggcggc
    22801 ggcgcgacat tcgccgatgc ccacgctccc gcgcttgtcc gcgccaaggg gcacacggtc
    22861 gccctcctcg cgtacacgaa gctcgtgccg ccgtttttga gcggcaatga ttccgccccc
    22921 gcggtcgcat atcccgatcc gacgcagatc gccgcggata tcgccgccgc gaagaaagaa
    22981 ggcgcggaca tcgtaatcgt ctccttccat tggggaacgg aatattcgac aacgcacgat
    23041 gcggcgcagg aagcgcttgc gcacgccgcg atcgacgctg gcgcgcttat cgtcatgggg
    23101 catcatccgc atgtggcgga ggaaatcgag cggtacaagg gcggcctcat catgtacagt
    23161 ctcggcaatt tcgtattcga ccagaatttc tccgccgata cgtcgcacgg cctcctgatg
    23221 cgcgtcgttg cggacgggaa ggggatagaa tcggtagagc ctctcgaagt gaaattcaac
    23281 gcctcgtacc agccggtcat agccggccca tggccgcaga aagcgaatgt gttatagtgg
    23341 ggccattaca actaagacag tcagacaatg aagaaaaaaa ccattttcat aaccgttgcc
    23401 gtcattgccg ttgtcgcggc gatcgtcaca gctgtcacga ttgcacctca aaagagcggc
    23461 gcgtatgatc agttcgctca atgcatcaag gattcaggca cgaccttcta cggcgcattc
    23521 tggtgccccc attgccgcga tcagaaagct gaattcggaa agtcggcgaa atatcttccg
    23581 tatgtagagt gctcgacttc cgacggccag tcgcagaatg cgacatgcaa tgcggcggga
    23641 atccagggct atccgacatg ggtctttccc gatcactcga cgaccaccgg cgttcagacg
    23701 cttcaaacgc ttgccgaaaa aacgagctgt ccgctgcctt cggcgcaata attttccatg
    23761 accggcttca ctgagattct caccgtaatt ctatcctcgc tcgcggtcat cgcgcttctc
    23821 ggcgcgcttg tcgcctttta tgcggcgtat gcccgcagtt cgatcggcgt gcgcattcgg
    23881 gaatacatcg gccccaagtc gctcgttctt gcgctcctcg tcgcggcggg cgccgccgca
    23941 ggcagcctcc tgtattcgta tgccgcaggc ttcgtcccat gcgatctgtg ctggtatcag
    24001 cgcgtgtttc ttttccctca agtcgtcatt ctcgcggttg cgctcgcgaa aggggatcgc
    24061 ggcgctggaa gatacggcct cgcgctctct attccgggag ctctgattgc cctctatcat
    24121 acgtatatcc agcttgcggg atccggcacc ctcctgccgt gcgccgccgc gggcgccgca
    24181 tgctcgaaag tctattttct cgaatggggc ttcatcacca tcccgacgat gtccctcatc
    24241 gcgttcctcg cgctcatcgc cttctatctc gtgcacggaa aggcctcgcg gagcatcaag
    24301 aggcttatct gatcgtgggc atcggcgcgc atgctcttca gttccgttca cgcgggcttc
    24361 attcggcatc gttatgatcc tttcatataa tccttaatcg gcaatccgtc gggaatgccc
    24421 gcgagtgaaa tgaaaagatt cgtcgttatc ggaggaggaa cgggcagcca tgccgtgctt
    24481 cgggggctta agcggtatcc gcttgacctc actgccgtcg tctccatgtt cgattccgga
    24541 ggctcgacgg gaatgctccg cgacgaattc ggcatattgc cgccgggcga cgtgcgccgc
    24601 tgcctggtgg cgcttgccga agagaacgcg ccgcgcggca acggcaggcg gggcggaccc
    24661 aatatccttc gcgagctttt caattatcgc ttcagcgaag agtcgtcctt gaaggggcac
    24721 agcatcggca acctgttcct gaccgccctt acgcagatcc tggggaacga ggcattggcc
    24781 atcagggaag cgggcaaggt tttgaatatc aaggggcggg tgcttcccgt atcgcttacg
    24841 aacgcgcatc tccatgctga gctcgaagac ggcaccgtca tccggggaga gaccaatatc
    24901 gatataccga agcatgacgg atcgaagcgg atcaggcgcg cgtttctcga gcctgcggca
    24961 ttcgccttcg aagagaccga ggccgcgatc agaaacgccg acgcgatcat catcggcccc
    25021 ggcgatcttt atacgtcgat cattccgaat ctcctggtga agggcattcc cgaagcgatt
    25081 cgagaaagcc gggcgacgaa gattttcgtc tgcaacgtca tgaccaagtg gggcgagacg
    25141 aacggcttca aagcgtcgga ttttgcgcgt gaagccctct tctattcagg cctcgacgcg
    25201 ttcgattatg tcatctgcag ttcgacgccg ctttctctcc cgacgctccg gcgctattcg
    25261 gaggaaaaat cgtttcccgt catctgcgac ccgctgctcg ggaattttgc ccgacgggtg
    25321 gtgcgcgagg atgtcgcact cgcttccgac atcatccgcc acgactcgga gaagctcgcg
    25381 tcggtcatta tcgcccaatg ccatgaaaat gcttatctcg gatttggacg gaacgctctc
    25441 gagcgcgacc ggccggtcgg atagccgccc ggattccttg cggcttctcc gcccgcgcga
    25501 aggcgcgctt gagctcctca cgcttccgaa cttcaggaac ttcctcgtct caacgggcga
    25561 gatccatacg gagacgatca agctgggtgt cttgaagttc ggccatctct ttcacggcat
    25621 atacaccgtg cattcggagc gcgacaagcg cttcgtcttc gaagagctgt ttgctacgca
    25681 cggaaggggg ctc
//
LOCUS       DASZYS010000046         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_501559, whole genome shotgun sequence.
ACCESSION   DASZYS010000046 DASZYS010000000
VERSION     DASZYS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501559"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHF05_02930"
     CDS             <1..289
                     /locus_tag="VHF05_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVS79909.1"
                     /translation="LSDRASLAADAGLLFIFPQPDYIGIWMKDMNFPIDVIWLDSTMK
                     VIYIVPNMTPESYPKVYTPTSPAAYVLEVNAGVAARENIKIGDQAKIIPGV"
     gene            547..3369
                     /locus_tag="VHF05_02935"
     CDS             547..3369
                     /locus_tag="VHF05_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HVS79910.1"
                     /translation="MSIFITKHDGTKEPFNADRINRSIERAARGLTNPVGMVTQIATE
                     TRLTLYDGITTDEMDQATINAAVQNIKEDIEYDKVAVRLLLKTVYRQVLGDYDKDDSG
                     DLAEKHRDGFITYIQEGVADGRLHPDMAAKFDLPKLAEALRVDRDELFDYAGLDSLLN
                     RYALKGIDQAPKETPQYFFMRIAMGLSYNESDPTLWAIRFYNKMSRLEYIAGGSTNLA
                     AGTTKPSLSNCFLLEIHDDMGHIAKSVSDVMLLSKGSGGIGASITKLRAAGSPLKSNN
                     GTSTGPTPFAKVIDTAIRAIQRGGKKKGALAFYMENWHLDFPEFLDWKQNNGDDYMRM
                     RTANTAVFISDEFMKRVDAGDDWYMFDPAETPDLNELYGSAFSKRYKEYVAMAEAGQL
                     RAFKKVPASEQYRQILVSLQTTAHPWLTWKDTINNRALNNNRGTIHMSNLCTEICLPQ
                     DKDNIAVCNLASLNLSAHVSNKEVNWSRLEESVRFAIRQLDNLIDINQLPVPEAARSD
                     RENRAIGLGIMGFADTIERLGMSYDSEHAWDFADRIFEFVSYMAIDESANLAKERGSY
                     MHFQGSEWSKGKVPVDTLAKVEEDRGVPLTVSRESKHKGLNWDVLRAKVKNGMRNSTL
                     MALAPNANIGLVAATTPGFDPRFAQVFSRNKISGKYLDINKNLVKDLKNMGLWDKVKD
                     QIIESQGDIAGIDGIPQHLKDIYKTSFTTSPYAFIEVAARAQKWVDQGLSRNMYLDTR
                     DNDEIMKIYKTAWEKGVKTTYYLHMKPRHTAEQSTVAVNKAQQIGKMGFAAAFGKKDA
                     APQPFSAPLQKTLELEPVTIAPAPEPVVAEAAPVASAPTAAPVKKEEPAAPVRATGGF
                     ASLSSLKKLADAPAPAEKKEIPLAAALNSSEASAKPTEKQEREKVAVPVSSMHAEAQA
                     AEAPKKKYNLILPEDPGAADICIGCE"
     gene            complement(3714..4253)
                     /locus_tag="VHF05_02940"
     CDS             complement(3714..4253)
                     /locus_tag="VHF05_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79911.1"
                     /translation="MLIILAGMACALLIQFFRLKRPLSHEAQKMISDLGPRYYFRYQM
                     AVAAAVGLMTGVAVAALLGAILPKRTFVEDTQELVSFLETEGADKAFFMDISLREGDP
                     FGFYYVRTDQGFSPQSRFIDDRTSIIESDKKSASLRRRYWTFSKRKYWLLGVTFPLPH
                     YEFVIPQAGIRRNIVYKAP"
     gene            4415..5449
                     /locus_tag="VHF05_02945"
     CDS             4415..5449
                     /locus_tag="VHF05_02945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011096638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HVS79912.1"
                     /translation="MALIGKASPEDMNLHPLNYKWAYDLYNQAVRNTWFPHEIALKED
                     IEDWEKMTEDERHAVKFLMAFFNPAELIVNRSIALGIYPYLKSPECHLYLAKQMWEEA
                     NHCVAFEYVLETFPFDRDTIYDVHLEVPSMQAKEAYINRYMKRMTEDTLDISTVEGKK
                     DFIRNLVATNIVMEGTWFYSGFMVALSFRQRNQLRNFGSMINWVIRDESLHLKFGINL
                     IHNILEENAELLTEEFANEIRSIIIEGVNLETDYNKDLFPNGILGLNADYVNQYVQYV
                     ADRRLEELGLPKHYNVTNPAKWMSTATDVFELVNFFEAQNTSYEVDARAHSADTAEKK
                     EAPAEPAQGE"
BASE COUNT         1342 a         1582 c         1465 g         1145 t
ORIGIN      
        1 cctctccgac agggcttctc tcgccgcgga tgccggtctt ctctttatct tccctcagcc
       61 cgactacatc ggcatctgga tgaaagatat gaattttccg attgacgtga tctggctcga
      121 cagcactatg aaagtcatct atatcgttcc aaacatgacg cctgaatcgt atccgaaagt
      181 ctatactccg acgtcgcccg cggcgtacgt gctcgaagtg aacgcgggcg tcgccgcgcg
      241 cgaaaatatc aaaatcggcg accaggcaaa aatcattccc ggggtgtgaa aagaatccta
      301 tttttcgcct tcgatcggcg tcgggcctta catgctcggc atttttgtta tccggtgtga
      361 tgtccacatt ttttttgccg aagcgcagga gtaaaatatt aaaaccttaa cggaaacgga
      421 gaatttttgg cggttttttc acggttttcg catattttta tgcaaaaata ggaagaaact
      481 gcttattttc atcttatccg catacggcgc acgttttatc aaatttagtt tctagcttca
      541 gcgatcatga gtattttcat caccaagcac gacggtacga aggagccgtt taacgcggac
      601 aggatcaacc gatccatcga gcgcgccgcc cgcggcctca cgaaccccgt cggcatggtg
      661 actcagatcg cgaccgaaac gcgccttacg ctctacgatg gcatcacgac cgacgagatg
      721 gatcaggcga ccatcaacgc ggccgtccag aacatcaagg aggacatcga gtacgacaag
      781 gtggccgtgc gcctgctttt gaagaccgtg taccgccagg ttttgggcga ttatgacaag
      841 gacgactcgg gcgacctcgc cgagaagcat cgtgacggct tcattaccta tatacaggaa
      901 ggcgtcgccg acggacggct ccacccggac atggcggcga agttcgacct cccgaagctc
      961 gccgaagccc ttcgcgtcga ccgcgacgag ctcttcgact acgccggact cgacagtctc
     1021 ttgaaccgct atgcgctcaa gggcatcgac caggcgccga aagaaacgcc gcagtacttc
     1081 ttcatgcgca ttgccatggg cctttcgtac aacgagtccg atccgacgct ctgggctatc
     1141 cgcttctaca acaagatgtc gcgcctcgag tacatcgctg gcggctcgac caatctcgcc
     1201 gcaggcacca cgaagccctc gctctcgaac tgcttcctcc ttgaaatcca cgacgacatg
     1261 ggccatatcg ccaagtcggt ctccgacgtg atgcttctct cgaagggctc gggcggcatc
     1321 ggcgcctcta tcacgaagct ccgcgccgcg ggctcgcccc tgaagagcaa caacggcacc
     1381 tcgaccggcc cgacgccatt cgccaaggtc atcgacaccg ccatccgcgc catccagcgc
     1441 ggcggcaaga agaagggcgc gctcgcgttc tacatggaga actggcatct cgattttccg
     1501 gaattcctcg actggaagca gaacaacggc gacgactaca tgcgcatgcg caccgcgaac
     1561 accgccgtgt tcatctcgga cgaatttatg aagcgcgttg acgcaggcga cgactggtac
     1621 atgttcgacc cggccgaaac tccggacttg aacgagctct acggctcggc attcagcaag
     1681 cgctacaagg aatacgtcgc gatggccgaa gcgggccagc tccgcgcctt caagaaggtt
     1741 cccgcgtccg agcagtatcg ccagatcctc gtttcgctcc agacgaccgc acatccgtgg
     1801 ctcacgtgga aggacacgat aaacaatagg gccctcaaca acaaccgcgg caccatccac
     1861 atgtcgaacc tctgcaccga aatctgcctg ccgcaggaca aggacaacat cgccgtctgc
     1921 aatctcgcct cgctcaacct ttccgcgcac gtttccaaca aggaagtgaa ctggtcgcgg
     1981 ctcgaggagt cggtgcgctt cgccatccgc cagctcgaca acctcattga catcaaccag
     2041 cttccggtgc cggaagccgc gcgctccgat agggagaacc gcgccattgg cctcggcatc
     2101 atgggcttcg ccgataccat cgagcgcctc ggcatgagct acgactccga gcatgcatgg
     2161 gatttcgccg accgcatctt cgagttcgtg agctacatgg cgatcgacga atcggccaat
     2221 cttgcgaagg agcgcggcag ctacatgcac ttccagggat cggagtggtc gaagggcaag
     2281 gtgcccgttg acacgctggc gaaagtcgag gaagaccgcg gcgtgccgct caccgtctcg
     2341 cgcgagagca agcacaaggg gctcaactgg gacgtgctcc gcgccaaggt gaagaacggc
     2401 atgaggaact cgaccttgat ggcgcttgct ccgaacgcga acatcggcct cgtcgccgcg
     2461 acgacgccgg gcttcgatcc gcgcttcgcg caggtcttct cgcgcaacaa gatttccggc
     2521 aagtatctcg acatcaataa gaatctcgtg aaggacctga agaacatggg gctgtgggac
     2581 aaggtgaaag accagatcat cgagagccag ggcgacatcg cgggcatcga cggcatcccc
     2641 cagcacttga aggatatcta caagacgtcg tttactacct cgccgtacgc cttcatcgaa
     2701 gtggcggctc gcgcgcagaa atgggtcgac cagggcctct cgcgcaacat gtatctcgat
     2761 acgcgcgaca acgacgagat catgaaaatc tacaagaccg catgggagaa gggcgtgaag
     2821 accacgtatt atctccacat gaagccgcgg cacacggcgg agcagtcgac ggtcgcggtc
     2881 aacaaggccc agcagatcgg caagatgggc ttcgcggcgg cattcggcaa gaaggacgcc
     2941 gcgccccagc ccttcagcgc gccgcttcag aagacgcttg agcttgagcc cgttacgatc
     3001 gctccggcac ccgagccggt tgtggccgaa gccgcgcctg tcgcgtccgc gcctactgcc
     3061 gctccggtca aaaaagaaga gcctgcggca cccgtacgcg cgaccggcgg attcgcaagc
     3121 ctctcgagcc tgaagaagct cgcggatgct cccgctccag ctgagaagaa ggaaatcccg
     3181 ctcgcggcgg cgctcaattc aagcgaagct tcggcgaagc ctacagagaa gcaggagagg
     3241 gaaaaggtgg cggtgccggt ctcttccatg cacgctgaag cgcaggcggc ggaagctccg
     3301 aagaagaagt acaatctgat attgccggag gatcctgggg ccgccgacat ctgcatcggt
     3361 tgcgaataaa gggccgctcg gacacattga tttcaaaaca cgataaaatc cagcccggtc
     3421 ggctggattt ttctatactc aggtgatttt ctactaaaaa tcaccctccg taatgttttg
     3481 aaatcaagaa tcctcgctct agtgttcaga aggaacgccc gcgcgggaca tgcgggctac
     3541 gggcgtgcat tgataaggat tcttacgcct cgaacttcaa gaaaaatcct cgggattctt
     3601 tgctcaaagt ttgctccttg aattcgagca aaaatataac gtaaatccta ataagatttt
     3661 aaaaaagacc cgtcggacat tgctgttcgg cgggtcgatt gtcgactgaa tcatcaaggt
     3721 gctttataca cgatattcct tctgatgccg gcttgaggaa ttacaaattc atagtgcggg
     3781 agaggaaaag ttactcccaa gagccagtac tttcttttgg agaatgtcca gtatctcctc
     3841 cttaaagacg cgcttttttt atccgattca atgatgctcg tccgatcatc gatgaatctg
     3901 ctctgagggg aaaaaccctg atctgttctt acgtagtaaa aaccgaatgg atctccctcc
     3961 cgcaagctga tatccataaa gaatgctttg tccgctcctt ctgtctcaag aaagctgacc
     4021 agttcttgcg tgtcttctac aaacgttctt tttggcaata tcgcgccgag aagcgcggct
     4081 acagcaacac ctgtcataag accaacagct gctgccactg ccatctgata ccggaagtaa
     4141 taacgggggc ccaaatcaga aatcattttt tgcgcttcat gcgacaaggg ccgtttcaga
     4201 cgaaagaatt gaattagcaa agcgcaggcc attcctgcga ggataattag cataatctgc
     4261 ctccatcatc aaaataaatt taaattagtt gtttgtcaat ttttctccaa tagcgtaggg
     4321 aaacggggga gcgtcaatag gcttttgcgg ttcgcgtttc gtataataag ggaactcaaa
     4381 atttaggaac ttatcagcta attccaacaa cacaatggca ctcatcggca aggcttcccc
     4441 ggaagacatg aatcttcatc cgctcaacta caagtgggcg tatgatctct acaatcaggc
     4501 ggtccgcaat acgtggtttc ctcacgagat cgccttgaag gaagatatcg aggattggga
     4561 gaagatgacg gaggacgagc ggcatgccgt gaagtttttg atggcgttct tcaaccccgc
     4621 ggagctcatc gtcaaccgct cgatcgcgct cggcatctat ccgtacttga agagtcccga
     4681 gtgccatctc tatctcgcca agcagatgtg ggaggaggcg aaccactgcg tcgctttcga
     4741 gtacgtgctc gagacgttcc cgttcgaccg cgacaccatc tatgacgtgc acctcgaagt
     4801 gccgtcgatg caggccaagg aggcgtacat caaccgctac atgaagcgca tgacggagga
     4861 cacgctcgac atctcgacgg tcgaaggcaa gaaggacttt atccgcaatc tcgtagcaac
     4921 gaacatcgtc atggaaggca cgtggttcta ctcgggcttc atggtggcgc tcagtttccg
     4981 ccagaggaac cagctgcgca acttcggctc gatgatcaac tgggtcatcc gcgacgagtc
     5041 gctccacctc aagttcggca tcaacctcat ccacaacatc ctcgaggaga acgccgagct
     5101 tctcaccgaa gaatttgcga acgagatccg cagcatcatc atcgaaggcg tgaatctcga
     5161 gaccgattac aacaaggacc ttttcccgaa tggcatcttg ggcctcaacg ctgactatgt
     5221 gaatcaatat gttcagtatg tggcggaccg caggcttgag gagctcggac ttcccaagca
     5281 ctacaatgtg acgaaccctg cgaagtggat gtcgaccgca accgacgtct ttgagcttgt
     5341 gaacttcttc gaagcgcaga atacgagcta cgaagtcgat gcccgcgccc attcggcgga
     5401 tacggcggaa aagaaggagg ctccggccga accggctcag ggagaatagg gaagttgcta
     5461 gttgttagtt tctagttatt agtttgcagg aaaatggcgg cacaaaacct ttcgatatat
     5521 cgaaaggttt tgtg
//
LOCUS       DASZYS010000047         6842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_566173, whole genome shotgun sequence.
ACCESSION   DASZYS010000047 DASZYS010000000
VERSION     DASZYS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566173"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VHF05_02950"
     CDS             <1..758
                     /locus_tag="VHF05_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HVS79913.1"
                     /translation="DDLTTSTVDDSTSNGNDGTKVAANHPAEGSGYISKGQFYADSTS
                     YYINSGTASSLNTTNSFTISGWMNFTDFGTGLATPFEALMWKGDGNTDSTINYFAETA
                     PGSVMRVGYAVSGSYKILDSTVALSSSRWYFITGTYDGTSNVLSLYIDGDLNTSGVQN
                     ADPSTNTSPLTIGSCSVSGNCDNMTGTIDEVHISNVARSAGWVKTEYNNQLSPSSFVA
                     LGGRQSQGRISYTSSSSVSTAPAYKIRGGVKFR"
     gene            865..2865
                     /locus_tag="VHF05_02955"
     CDS             865..2865
                     /locus_tag="VHF05_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVS79914.1"
                     /translation="MPTGRKFAYLFTSVALLAGPIFVPFEPSYISVAGNTALRAAEYL
                     AGAAGPFLSAAAVEFQDSFQSAWTGKQAPKASVAATGMAEKEPAGPLVSNGDFEGGRS
                     GWTFYTDAIGIFAVEAGKDGTAAKILVSKPGSSTELFHPGIRIDPGAEYRLSFSARSS
                     LAHDVSVSVMSGDESKPYGAGGRVFDLSPDWQDFSFDFTASGLESAVSDARLIFQLSP
                     YASIGDEYWFDDVSLERIDSSDASSTLSSAPAKFESAAMDISGIPQGVAIADMNGDGI
                     PDFIASSGGTAENGASGIGWYEGLKGSNAPGAKHEVFQGDVSGSIAAYDVNGDGARDI
                     IATSGGSIWWFENPGSAAGASSAWKKHYIGAGNADGEIHIADIDGDGKKDIVAGAIVY
                     FQDDIYDWTPRRIGSATGQSALLSIDSWNGAINLVATHGGTFVPAWYENPREYGGNAR
                     ADVWSVHDLMPDSDFPSCTVAACVGRIGAFATADMNGDGRQDIVSGEAGPDSSTPLGG
                     LTWWEAPQGREGDWTKHVIDGSLGAASFISVGDLNGDGSPDIIASGQSPSGPRSALIY
                     NDGTGNFPFHDFSLGNADIFAVTPVAPGKAPSIFAGVGGADGLKEYLNQNRVFVPPAA
                     AKPPEIATSTAKQEGDTEPAPSSPTAPLLQIDASATLSLPSL"
     gene            3602..5983
                     /locus_tag="VHF05_02960"
     CDS             3602..5983
                     /locus_tag="VHF05_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVS79915.1"
                     /translation="MEETHSSAAPAAPLRLEKIAAIVLMATAFITPIFFIPSGLFSVP
                     FAKSIVLYIGVILSFILFLFSMLESGVVKIFKSPIFYLGFAIPVLFLVSSLSSSSVMT
                     SLSGYGVEVGTFSFVLLMYLMMCLVPALYRTKERIFYTYLLYFLSFGIVALYQTLRLI
                     FGPNFLSFGLMTTSASNLVGSWNDLGVFFAIAALLAVMTLEMLSLSRLFKWLLYVSLV
                     LSLLFVSIVNFLTLWYILAAFSLVFLLFLMFSRRERHSSEGHEAHGRRLSYLSIALFV
                     LSLIFIFAGSFLGNGIANALHVANLEVRPSWSTTWSVARQSLSHNILLGSGPNTFQYD
                     WVKYKPAAINSTAFWSTDFMYGIGLIPTFLATSGILGFLAWIGLLAMLVYTGVRTIFM
                     EQHDSLSRYFVVSSFIASLCLWATLIFYVPSMAMVAITFLFTGLFIASVANLGLLGHK
                     TIHLYNNARTGFTAVLVTVFALLLAIAGGYFMTRNFVSTGYFQKSVQEASANNITGAE
                     ADMTNAINLRGFDIYYRSLAEIQMVKLNAVLNQPASSTSADALRQQFQTTLGSAIQSG
                     LNAVTADPANYQNYIELGQIYEAVVPPPFQIDGAYDKAKEEYQKAIALNPQGPEPRLL
                     AGRLEMAKGNTDAARTAINEAIQLKPDYVDAFYLLAQVEATAGNLDKAISSAQSATYL
                     APDNAGVYFQLGLLKYDNNDFSGASQAFEQALKIVPNYANAQYFLGLSYAKLNRTDDA
                     IKQFEAVAASNPDNAEVKTILSNLRAGLAPLANLSASEQEPQKRDTPPIKQDQ"
     gene            5999..>6842
                     /locus_tag="VHF05_02965"
     CDS             5999..>6842
                     /locus_tag="VHF05_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HVS79916.1"
                     /translation="MVKKIFQFLQKEISGLHSAAYLLAMSALLSQILGLLRDRLLASY
                     FGAGNTLDIYYAAFRIPDFLFISVGSMVSVAVLIPFMMERMKSGPEEGKRFLSNVFSF
                     FSLFIVVVCGIAFFLIPVLSAKIFPGFDASSLAKVVMLTRIILLSPIFLGLSNLLGTL
                     TQMYQRFFLYSLSPIVYNIGIISGVIFFYPIFGIPGLAYGVVLGALAHFAVQAPFIVG
                     HGLFPRLTLRFDMKEIKKVFLVSLPRTFALGSDNISVMFLLSIASLMASGSIAIFSFS
                     YNLQS"
BASE COUNT         1367 a         2052 c         1848 g         1575 t
ORIGIN      
        1 tggacgacct cacgacgtcg accgtcgacg actcgacgtc gaacggcaat gacggtacga
       61 aggtcgccgc aaaccatccg gcggaaggct ccggctacat aagcaaaggc cagttctacg
      121 ccgactcaac gagttattac attaatagcg gcactgcaag cagtctcaac accacgaaca
      181 gcttcacaat ttcaggctgg atgaatttca ccgatttcgg caccggcctc gcgacgccct
      241 ttgaggcgct catgtggaaa ggagacggta acaccgacag taccatcaat tacttcgctg
      301 aaacggctcc cggcagcgta atgcgcgtgg gatacgcggt cagcggatca tacaagatcc
      361 tcgacagcac ggtcgccctc agttcgagta gatggtattt catcaccggc acctacgatg
      421 gcacgtccaa tgtgctctcg ctctacatcg acggcgatct caatacttca ggcgtccaaa
      481 acgccgaccc gtcgacgaac acttcgccgc tcaccatcgg ctcatgttca gtttcgggaa
      541 attgcgacaa catgaccggc acgatcgacg aagttcatat ttccaacgtt gcccgctccg
      601 cagggtgggt caagaccgag tacaacaacc agctctcacc atcgtcattc gtcgccttgg
      661 gcggcaggca gtcacagggc agaatctcgt acacttcttc atcgtccgtt tcgacagcgc
      721 ctgcctataa gatacgcggg ggagtcaaat tcaggtaaaa aatggcgcac tcgtctttgg
      781 tatgcccttc tcaagggatc gggcatggct ctaggatagt gcaaacgggc gtgaggcgga
      841 tataatgggg gaaattacgc tcccatgcct accggaagga aattcgcata tctcttcacc
      901 tccgtcgcgc ttcttgccgg gccgattttc gtgccgttcg agccgtcgta catctccgtg
      961 gcgggaaata ccgcgcttcg cgcggcggag tatcttgcag gcgccgcagg tccgttcctc
     1021 tcggcggcgg cggtcgaatt ccaggattca ttccagagcg cgtggaccgg aaagcaggcg
     1081 ccgaaagctt cagtcgcggc gaccggcatg gcggagaaag agccggcagg ccccttggtc
     1141 tcaaacggcg atttcgaagg aggaagaagc ggctggacgt tctacaccga tgcgatcggc
     1201 atcttcgccg tcgaagcggg gaaggacgga acggccgcga aaatccttgt ttcgaagccc
     1261 ggatcctcca ccgagctttt ccaccccggc attcggatcg atcccggcgc tgagtaccgg
     1321 ctttcgtttt cggcgcgatc ttctctggcg cacgacgtgt ccgtttccgt gatgagcggc
     1381 gacgaaagca agccgtacgg agcgggcggc agggtgttcg acctttctcc cgattggcag
     1441 gatttcagtt tcgatttcac cgcaagcgga ctggagagcg cagtttccga tgcgcgatta
     1501 atctttcagc tcagtccgta cgcttcgatc ggcgacgaat attggttcga tgacgtgtcg
     1561 ctcgaaagaa tcgattcctc agatgcgtcc tccacgcttt cctcagctcc ggcaaagttc
     1621 gagagcgcgg ccatggatat ttccggcatt cctcaaggag tcgcgattgc ggatatgaac
     1681 ggcgacggca ttcccgactt catagccagt tccggcggga cggcggaaaa cggcgcttcc
     1741 ggtatcgggt ggtatgaagg cttaaagggc tcgaatgccc cgggagcgaa gcatgaggtt
     1801 tttcaaggcg atgtctccgg ttcgattgcc gcatacgatg tgaacggcga cggcgcgcgc
     1861 gacatcattg ccacttccgg aggaagcata tggtggtttg agaatcccgg cagtgcggca
     1921 ggcgcgtctt ccgcatggaa gaagcattac atcggagcgg gaaatgccga cggagagatt
     1981 catatcgccg atatcgacgg cgacggtaaa aaagatatcg tggccggcgc gatcgtatat
     2041 ttccaggatg acatatacga ttggacgccg cgccggatcg gcagcgccac ggggcagtcc
     2101 gcgcttctct cgatcgattc gtggaacgga gcgatcaatc tcgtcgcaac tcacggcggc
     2161 acattcgttc ccgcatggta tgaaaatccg cgcgaatacg gaggaaatgc gcgcgccgat
     2221 gtgtggtcgg tgcacgatct tatgccggat tcggattttc cgtcatgcac ggtcgcggca
     2281 tgcgtcggac gaatcggcgc attcgcgacg gccgacatga acggcgacgg caggcaggat
     2341 attgtctcgg gagaagccgg accggactcg agcacgccgc tcggcgggct cacctggtgg
     2401 gaggcgccgc aggggaggga aggagattgg acgaagcacg tgatcgacgg atcgctcggc
     2461 gccgcatcgt tcatatccgt tggggatttg aacggcgacg gctctcccga tattatcgct
     2521 tcgggacagt cgccttcggg cccgcgctcc gcgcttatct acaacgacgg tacggggaac
     2581 tttccattcc acgatttctc gctcgggaat gccgatattt tcgctgtcac gcccgtggcg
     2641 ccaggaaagg cgccgagcat ctttgccggt gttggcggcg cggacggact taaggaatat
     2701 ctgaaccaga acagggtgtt cgtgccgccg gcggcagcca aacccccgga aatcgcaacc
     2761 tccaccgcaa agcaggaagg ggataccgag cccgctccgt cttcgcctac ggctccgctc
     2821 cttcagatcg acgctagcgc cactctttca ctaccttccc tttaaatttc aagacgtgcc
     2881 atgtagataa ttttgtggta tagtaataac gctcatgccg gatagctgct cttatttcca
     2941 tgtgaaatgg gggaggaaag tccgaacatt cagccggggc ttacgccccg gcacgggtag
     3001 cgggtaacac ccgtccgccg agcaccactt ttgatcgaca gaattatatg aggatttgca
     3061 aagtgtttgc aatttttctc aaataaaaat ggatacttgt ttatcaaaag tggtgctcgg
     3121 cgagaggtgc gaacagcgac gcctgaccgg cgaaagccgg cggctcccgc cgagcgccag
     3181 caatggcgtt cgacgggatg aatccggaga gcgatcttcg gggtgaaacg gcaaaatcct
     3241 tatctgaatg caaggccgag ccccccacca cttctgacat tgtgcgaagc acaagttgga
     3301 aacgatgtca gaagtggtgg ggggagtgcc gcttgatccg cctggcgaca ggcgagacag
     3361 ataaatggct atcggccgcc tgcaaaggcg gctacagaat tcggcttata ggcatgagca
     3421 aaaaatgaca aaggcccgct tcatgcgggc ctttgtgcta tctgttccgc agtttttctt
     3481 cacaggtctt ccattgaaga gcgccgtcca tttgctataa tttcaaatgc acaagggctt
     3541 tccggagctt gctccgggca tcaccgcttt taccagatag ttcgttattt ttatttttcg
     3601 tatggaagaa acacactcat cagccgctcc ggcggctccg ctccggctgg aaaagatcgc
     3661 cgcgatagtc ctcatggcta ccgcattcat cacgccgata tttttcattc cctcgggcct
     3721 tttttcggtc ccgttcgcca agagcatcgt gctctatatc ggagtgatcc tttccttcat
     3781 cctcttcctt ttctcgatgc ttgaaagcgg cgtcgtaaaa atcttcaaaa gccccatttt
     3841 ctacttagga ttcgcaattc ccgttttgtt ccttgtttct tcgttgtctt cctcgtcggt
     3901 gatgacctcg ctttccggat acggcgtcga agtgggaacg ttctcgttcg tgcttctcat
     3961 gtatctcatg atgtgcctcg tcccggcgct ctataggacc aaggagcgca tcttctacac
     4021 ctatcttctg tactttctct cgttcggcat cgtcgcgctc tatcagacgc tccgcctcat
     4081 cttcggcccg aacttcctct cgttcggcct catgaccacg tccgcctcga atctcgtcgg
     4141 cagctggaac gacctcggcg tgttcttcgc catcgcggcg ctactcgcag tgatgactct
     4201 cgagatgctt tccttgagca ggctcttcaa gtggcttctc tatgtctcgc tcgtcctttc
     4261 gctcctcttc gtgagcattg tgaacttcct gacgctctgg tacattctcg cggcattctc
     4321 gctcgtcttt ctcctgttcc tcatgttctc gcgccgcgaa cggcactcgt cggaagggca
     4381 tgaagcgcac ggcaggcgcc tttcgtacct ttcgatcgcc cttttcgtcc tttcgcttat
     4441 ctttatcttc gcgggaagtt tcctcggcaa tggcatcgcg aacgcccttc atgtcgcgaa
     4501 cctcgaagtg cgtccctcct ggtcgacgac gtggagcgtg gcgcggcaat cgctctctca
     4561 caacatactt ctcggctcgg gccccaatac cttccagtat gactgggtga aatacaagcc
     4621 ggcggcgatc aactcgaccg cgttctggag caccgacttt atgtacggca tcggccttat
     4681 tccgaccttc ctcgcgacgt cgggcatcct gggcttcctc gcctggatcg gccttctggc
     4741 aatgctcgtc tatacgggcg ttcgcacgat attcatggag cagcatgatt cgctatcgcg
     4801 ctacttcgtg gtgtcgtcgt ttatcgcatc gctctgcctc tgggcgacgc tcatcttcta
     4861 cgtgccgagc atggcgatgg tggcgatcac attcctcttt accggcctct tcatcgcgtc
     4921 cgtggcgaat ctggggctct tgggccacaa gacgatccat ctctacaaca acgctcgcac
     4981 cggcttcacg gccgtgctgg ttaccgtctt cgcactcctt ctcgcgatcg cgggcggcta
     5041 cttcatgacg aggaacttcg tctcgaccgg ctacttccag aagagcgtac aggaggcgag
     5101 cgcgaacaat atcacgggcg ccgaagccga catgacaaac gcgattaatc tccgcggctt
     5161 cgatatctac taccgttcgc tcgccgagat ccagatggta aaattgaacg cggttttgaa
     5221 tcagcccgca tcgagcacca gcgccgacgc gctccgccag cagttccaga cgacgctcgg
     5281 ctcggctatc cagagcggct tgaacgcggt aacggccgac ccggcaaact atcagaacta
     5341 cattgaactc ggacagatct atgaggcggt tgttcctccg ccgttccaga tcgacggcgc
     5401 atatgacaag gcgaaggagg agtatcagaa ggcgatagcc ttgaatccgc agggccccga
     5461 gccgcgcctt cttgcaggca ggctcgaaat ggccaagggc aacaccgacg ccgcccgcac
     5521 cgcgatcaac gaggcgatcc aattgaagcc cgactatgtc gacgcgttct atctcctggc
     5581 gcaggtggag gcgacggcgg gaaatctcga caaggccatc agctcggcgc agtcggcgac
     5641 ctatctcgct ccggacaatg cgggcgtcta tttccagctc ggactcctga agtacgacaa
     5701 caacgacttc tcgggcgcct cccaggcttt cgagcaggcg cttaagatcg ttccgaacta
     5761 cgcgaacgcc cagtacttcc tggggctctc ctacgcgaag cttaaccgca ccgacgatgc
     5821 cataaaacag ttcgaagccg tcgcggcatc caacccggac aatgcggaag taaaaacgat
     5881 tctttccaat cttcgcgccg gcctcgcgcc cctcgccaat ctgagcgcct ccgagcagga
     5941 gcctcagaag agggacactc cgccgatcaa gcaggaccaa taagtcccac gcggatacat
     6001 ggtaaagaag atattccagt tcctgcagaa agagataagc ggccttcata gcgccgctta
     6061 tcttttagcc atgtccgcgc tcctctccca gatcctgggc ctcttgcgcg accggctgct
     6121 cgcgtcgtac tttggcgcgg gcaatacgct cgatatctat tacgcggcat tccgcattcc
     6181 ggatttcctt ttcatttctg tcggctcgat ggtgtcggtc gccgtgctca tcccgttcat
     6241 gatggagagg atgaagtccg gaccggagga agggaagcgg tttctctcga acgtattctc
     6301 gtttttctcg ctcttcatcg tggtcgtctg cggcatcgcg ttcttcctca ttccggtcct
     6361 ttccgcaaaa atctttcccg gtttcgatgc ctcgagcctc gccaaggttg tcatgctgac
     6421 gcgcatcatc ctgctctctc cgatatttct cggcctctcc aaccttctcg gtacgctcac
     6481 gcagatgtac cagcgatttt tcctctattc gctatctccc attgtctaca atatcggcat
     6541 catctcgggc gtcatcttct tctatccgat cttcggcatt cccggactcg cctacggcgt
     6601 cgttttggga gccttggcgc attttgccgt tcaggcgccc tttattgtcg gccacgggct
     6661 ctttcctcgc ctcacgctcc gcttcgatat gaaggagata aagaaggtct tcctggtctc
     6721 cttgccgcgc accttcgctc tcgggtcgga caacatctcg gtcatgttcc tcctttccat
     6781 cgcatccctg atggcctccg gctcgatcgc catcttcagc ttctcgtaca acttgcagtc
     6841 ag
//
LOCUS       DASZYS010000048         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_876616, whole genome shotgun sequence.
ACCESSION   DASZYS010000048 DASZYS010000000
VERSION     DASZYS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876616"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2468)
                     /gene="ppdK"
                     /locus_tag="VHF05_02970"
     CDS             complement(<1..2468)
                     /gene="ppdK"
                     /locus_tag="VHF05_02970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HVS79917.1"
                     /translation="MKYVYFFGAGKADGTGDMREVLGGKGAGLAEMTKIGLPVPAGFT
                     ITTEACDYFYKHDKKYPKELREQVKQNVAKLEKLTKKKLGDAKDPLLVSVRSGSARSM
                     PGMMETILNLGLNSKSVEGLAKATNNDRFAWDAYRRFVQMYSSVVIGLSKDELETRLR
                     NMKQYLGVKDDTEVTAQGWRQLVGEYKAYFKEKTGQDFPEDPIEQLWGAIGAVFGSWM
                     GEKAVTYRRVEKITGLLGTAVNVVQMVFGNTGEESGTGVCFTRDPSTGEKTFFGDFLL
                     NAQGEDVVAGIRTPMHLSELSKKMPKIYKQLEKVRDKLEKHYRDMQDMEFTVESGKLY
                     MLQTRTGKRSPAATFRIAVDMANEKLISTTEAISRVKPEDIERLFYPVLDQSFSRKDL
                     ESRKIAEGINAVPGAAVGKAVFTAEEAEHWAQNGEKVILVRRETSPEDVGGMHVAQGI
                     LTATGGKTSHAAVVARGWGKCCIVGADKIDIDVKSKKLSVNGYVIKQGDWLTLDGGDG
                     SVYKGEIPLTRPQPPKAYETLMKWCDKVRTLGVRTNADTPNDARVAREMGAEGIGLCR
                     TEHMFFKDFEFPEKSIERQEAIQEMILADSPEARRKALDKLLPFQRRDFTGIFEAMNG
                     LPVTIRLLDPPLHEFVPHDPAKQADLAKKIGISAEQVARRVEQLHEANPMLGHRGCRL
                     SITFPEILEMQVRAIIEAAIDCQKRGVKVLPEIMHPLVLDEKELKILETATRRVADKI
                     ISEAKVKLEYLVGTMIELPRAALLAGQIANVAEFFSFGTNDLTQTTMGLSRDDAGRFL
                     PEYVDENKAGIFKADPFQTIDQAGV"
     gene            complement(2752..>4119)
                     /gene="glyS"
                     /locus_tag="VHF05_02975"
     CDS             complement(2752..>4119)
                     /gene="glyS"
                     /locus_tag="VHF05_02975"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVS79918.1"
                     /translation="IMGDFDEKYLGLPEEILITVMRGHQKYFALESRRGQLAPHFLAV
                     INLPRDPKGLVQAGHERVLRARFADAQFFWDTDQKTKLGDYLPRLKAVTYESRLGTYH
                     DKVERMRSIARWLAEQLFSSGVKEADVAGCDRAAELAKCDLVTGMVGEFSELQGIVGG
                     LYAHAQGEPDEIADAVYDHYRPQGLEEDIPRNLTGCVVSFADKLDSIMACFAVGAIPS
                     GSSDPFALRRAALGIVKMILERNLPLSISATVSAAAKTLREQPPRIGASEEVQKQVLD
                     FILDRAKFILRERRGFAYDEINAVFASGADDLVDAVERVTAVRAIRNTRNFPPLAASF
                     KRIRNILEKSVGSQDKSYGGVKQDLLKDDFERQLHTAAQRIGEEAGRRRREKKYKAAL
                     EKISELRPAVDMFFDKVHVMAEDPEIRKNRIALLGGLLKEFSTIADFSELGGEEVKEG
                     REVRA"
BASE COUNT          879 a         1228 c         1021 g          991 t
ORIGIN      
        1 acgccggctt ggtcgattgt ctgaaacggg tcggccttga agattccggc cttgttctcg
       61 tcgacatatt ccggcaggaa gcggcccgcg tcgtcgcgcg aaagtcccat cgtcgtttgc
      121 gtgaggtcgt tcgtgccgaa agagaagaac tcggcgacgt tcgcgatctg cccagcaagt
      181 agagcggccc tgggaagctc gatcatcgtg ccgacaagat attcaagttt tactttagct
      241 tctgaaatta tcttgtctgc cactcgcctt gtagccgttt cgaggatttt caattccttc
      301 tcatccaaaa caagaggatg cataatctca ggtaaaactt taactccgcg cttttggcag
      361 tctatggctg cctcgatgat ggccctgacc tgcatttcga gaatttcagg gaacgtgatc
      421 gagagacggc agccacggtg gccgagcatt ggattcgctt cgtgcaattg ttcgacgcgg
      481 cgcgccacct gctcggccga gatgccaatt ttctttgcca aatcggcctg cttcgcggga
      541 tcgtgcggca cgaactcgtg cagcggcgga tcgagcaagc ggatcgttac gggcaggccg
      601 ttcattgcct cgaagatgcc ggtaaagtcg cgacgctgga acggaagcag cttgtcgaga
      661 gccttccgcc gcgcttctgg gctatcggca aggatcattt cctgaatcgc ttcctggcgc
      721 tcgatgctct tttccggaaa ttcaaagtcc ttaaagaaca tgtgctctgt gcggcagagt
      781 ccgatgccct cggcacccat ctcgcgggcc acgcgcgcgt cattcggcgt gtccgcattt
      841 gtacgaacgc cgagtgtgcg gactttgtcg caccacttca tcagtgtttc gtatgctttc
      901 ggcggctgcg gtcttgtgag aggaatctcg cccttgtaaa cggagccatc accgccatcg
      961 agcgtgagcc aatcgccctg cttgatgacg taaccgttca cactgagctt cttcgacttc
     1021 acatcgatgt cgattttgtc cgcgccaacg atgcagcact tgccccatcc gcgcgccaca
     1081 acggcagcgt ggctggtctt gccgcccgtc gccgtgagaa ttccctgcgc aacgtgcatt
     1141 ccgccaacgt cctcaggact cgtttcgcgg cgcacgagaa tcaccttctc gccgttttgc
     1201 gcccaatgct ccgcttcctc cgcagtgaac acagctttgc cgactgcggc gccagggact
     1261 gcattgattc cctcggcgat cttgcggctt tcgagatctt tccttgagaa cgattgatcg
     1321 aggacgggat agaacaagcg ctcaatatct tccggcttga cgcgcgaaat tgcctcggtc
     1381 gtcgaaatga gcttttcatt tgccatatcg acggcaatgc gaaatgtcgc ggcaggcgag
     1441 cgttttccgg tacgggtttg caacatatag agctttccgg actcgaccgt aaactccatg
     1501 tcctgcatat cgcggtaatg cttctcgagt ttgtcgcgaa ctttctcaag ctgcttgtag
     1561 attttcggca tcttcttgga gagttcgctc aggtgcatcg gagtgcggat cccagccacc
     1621 acgtcctcac cctgcgcatt caaaagaaaa tcgccaaaga atgtcttctc gcctgtactc
     1681 ggatcgcgcg taaagcagac accggtaccg gattcctcac ccgtgttgcc gaagaccatc
     1741 tggacgacgt tcaccgcagt accgagaagg cccgtaatct tctcaacgcg ccggtatgtg
     1801 acagcctttt cgcccatcca cgaaccgaac accgcgccaa ttgctcccca aagctgttcg
     1861 atgggatctt ccgggaaatc ctgccccgtc ttctccttaa agtatgcctt atactcgcca
     1921 acaagttgcc gccagccttg cgcggtcacc tcagtgtcgt ccttcacacc gagatattgc
     1981 ttcatattgc ggaggcgcgt ctccaattcg tcctttgaga gaccaatcac cacgctcgaa
     2041 tacatctgaa cgaagcggcg ataggcatcc caggcaaaac gatcattatt agtcgctttt
     2101 gcaagcccct ccacggactt actgttcagc cccaggttca ggatcgtctc catcatgccc
     2161 ggcatggaac gcgctgaacc cgagcggacg ctcacaagca gcggatcctt tgcgtctcca
     2221 agcttcttct tcgtgagctt ctcgagcttc gcgacatttt gcttaacctg ctcgcgcagc
     2281 tccttcggat atttcttgtc gtgcttatag aagtaatcgc acgcttccgt tgtgatcgta
     2341 aatcccgcgg gaacaggaag accgatcttc gtcatttcgg cgagtccggc gcctttgccg
     2401 ccgagaacct cccgcatatc tcccgtgcca tccgctttcc cagctccaaa aaaataaacg
     2461 tatttcattt tgctccgttc ctcgctgatt gagatgaatt cgattccttg ttccggttca
     2521 acatcagtat tcgattgcgt cgcccggccc cacaaccgag ctgaaaattt cttaaagaat
     2581 taagcgaaca tgtcggcgag ccgacaattg atgctttact tcaatactgc catatgcccc
     2641 cgcttcgaaa ggtaacgcct gaaaatcgcg atccacaact aaatctttgc tttcaattcc
     2701 tcaaatgtcg gcgggccgac atctcatgta taatcttgcg gcttcgacgt gttaggctcg
     2761 cacctcacgg ccttctttaa cttcttctcc gcccaactcc gagaagtcgg caatcgtaga
     2821 aaattcctta agcagtcctc cgagaagcgc aatcctgttc ttgcgaatct ccgggtcttc
     2881 agccatcaca tgaaccttgt caaagaacat gtccacggcc ggccgcagct cggatatttt
     2941 ctcaagcgcc gctttatatt ttttctcgcg cctccggcga cctgcctctt caccgatgcg
     3001 ctgtgccgca gtgtgaagct gacgttcaaa atcatcttta agtagatcct gtttcacgcc
     3061 gccataggat ttgtcctggc ttccgacgga cttctcgaga atattgcgga ttcgcttgaa
     3121 gctcgccgca agcggaggaa agtttctcgt attccgaata gccctcacgg cagtaacgcg
     3181 ctcgacggca tcgaccagat catcggcacc cgacgcaaag acagcattga tctcgtcata
     3241 ggcaaatccg cgtcgttcgc gcaggatgaa cttagcgcgg tccaggatga aatcgagcac
     3301 ttgtttttgg acttcctcgg aagctccaat tcggggaggc tgttcgcgaa gcgtttttgc
     3361 cgcagccgag acagtcgccg agatcgagag gggcagattt cgctccagaa tcatctttac
     3421 gataccaagc gcggcgcggc gcagagcaaa aggatcgctg gaaccactgg gaatcgcgcc
     3481 aacggcaaaa cacgccatga tcgaatcgag tttatctgca aacgaaacca cgcatcctgt
     3541 caaattgcgc ggaatatcct cttccagtcc ttgtggcctg taatgatcgt agacggcatc
     3601 ggcaatttca tctggctcgc cttgcgcatg agcatagagg ccgccgacga ttccctgcaa
     3661 ttcgctaaat tcgccaacca tccctgtcac aagatcgcac tttgcaagtt cggcggcgcg
     3721 atcgcaaccc gccacatccg cttccttaac accgctgctg aacaattgct cggcgagcca
     3781 tcgcgcaatc gaacgcatgc gctcgacttt gtcatgataa gtgccgagcc gggattcgta
     3841 tgtgacagcc ttcagccgtg gaagataatc gccaagcttg gtcttttggt ccgtatccca
     3901 gaaaaattgc gcgtccgcaa aacgcgcgcg taacacgcgc tcatgcccgg cttgcacaag
     3961 gccctttgga tcgcgcggaa gattaatgac cgcaagaaaa tgcggcgcca attggccacg
     4021 gcgcgactca agcgcgaaat atttctgatg gccgcgcatt accgtgatca ggatttcctc
     4081 aggcaggccg agatacttct cgtcaaaatc gcccataat
//
LOCUS       DASZYS010000049         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_249034, whole genome shotgun sequence.
ACCESSION   DASZYS010000049 DASZYS010000000
VERSION     DASZYS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249034"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(598..1173)
                     /locus_tag="VHF05_02980"
     CDS             complement(598..1173)
                     /locus_tag="VHF05_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HVS79919.1"
                     /translation="MKKIVFDIETRNTFQDVGSAESTALDISIVGVYDSSTDSYSTYT
                     VEELPKLWPILENADLIIGYNSEHFDLPLLNKYYHGDLSRLKHVDILKEIKQSFGRRM
                     KLDQVAEGTLGINKSGHGLDAIRWWRQGEIDKIRKYCLDDVRITKEVYEYALKNGGLK
                     FKEGGQVMDIKLDTSGWETPEEGGMMLTLPF"
     gene            complement(1270..2577)
                     /gene="hisS"
                     /locus_tag="VHF05_02985"
     CDS             complement(1270..2577)
                     /gene="hisS"
                     /locus_tag="VHF05_02985"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVS79920.1"
                     /translation="MEENQSKVSTESYKGVRDFYPEDKFVQDYMFGVMRKTARSFGYS
                     EYDASILEPAELYKTKSGEEIVNEQTYTFTDRGEREVTLRPEMTPTVARMVAARRREL
                     SFPLRWFSIPNLFRYERPQKGRLREHWQLNADIFGVDNSWAETEVIALASELLANFGA
                     KQDDFEIRVSSRKILNDIYKLYGFDKDQSYRISKLIDKKSKISAEEFAVQLREAAGEY
                     AAELETLLSTNGDIEAVKAGEGYRETMEVIERLNGLGVANTSFDVSLMRGFDYYTGII
                     FEIFDTDPENRRSMFGGGRYDDLLSIFGSEKVPAVGFGMGDVTLRDFLESRNLLPAYA
                     PSAKLYICVVDSASAERAETLAKELRNAGLSAAVDLTGKKLGDQLKKAEKDSVPYALI
                     IGENEVSSGKYKLKTLATREEEELGIREIIEKINQKSKIKDQN"
     gene            2826..3095
                     /locus_tag="VHF05_02990"
     CDS             2826..3095
                     /locus_tag="VHF05_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HVS79921.1"
                     /translation="MATKLYVGNMSYSTTEDGLREAFSKAGTVVSATVINDKITGRPR
                     GFGFVEMEDADAQKAIDMWNEKDLDGRNLVVNEARPMQPRQGGMR"
BASE COUNT          727 a          864 c          883 g          812 t
ORIGIN      
        1 gggagcggca gaaacatggg cacgggactg tattcccggg gtagccaaca gcatggcggc
       61 actgagtcct gtgaacacga ctccttggaa aagtttcttc aacttctcct cctttgtttg
      121 atttggctta ctaaggaacg gtaaagccag ggagtaattt acactaagct tttaatactg
      181 tcaagtattc gtgaacaggc tatcggagac aacatttttc gtatgcgaaa aagcgaaccg
      241 taacaggtcc gcttagtttg cgaagacaat cgcagtttgc gccaagctag attctagagc
      301 ttagcgttgc acgccaggtc ccaccagtgg cacgaattcg gatcgtgcgg ctgaggcaac
      361 cgagatccgt cgagcggttc tttcgggctt cgaggtcccg acagcggccc tttccggacg
      421 gttaccagcg acattggtga ggctgtcagc atgacggctg acagggatgc aactgcgaaa
      481 aaatggaaaa gtttcttcaa cttttcctcc ttttttgttt tttggctcca aggaacggta
      541 agccaggggc tactatatac cacccgtttc tgggtgtcaa gtaccccaga aactcagcta
      601 aaaggggagg gtaagcatca tgccgccctc ctccggagtc tcccagcccg acgtatcgag
      661 cttgatgtcc ataacctggc cgccttcctt gaacttcagg ccgccgtttt ttagcgcgta
      721 ttcataaacc tccttggtga tgcgcacgtc gtcgaggcag tacttcctga tcttgtcgat
      781 ttcgccctgc cgccaccagc ggatcgcgtc gaggccgtgg ccgctcttgt tgattccgag
      841 cgttccttcg gcgacctggt cgagcttcat gcggcggccg aagctctgct tgatttcctt
      901 caaaatgtcg acatgcttca ggcgggagag gtcgccgtgg taatacttgt tgaggagcgg
      961 caggtcgaaa tgctcggaat tgtagccgat aatgagatcg gcattttcga ggataggcca
     1021 gagcttcggc agttcttcca ccgtgtacgt gctgtatgaa tcggtggagg aatcgtacac
     1081 gccgacgatg gagatgtcga gcgccgtcga ttccgccgat ccgacgtcct ggaaggtatt
     1141 tcttgtttca atatcaaaaa caattttctt cattgcttca tggtcattaa aattttgccg
     1201 ataagatcaa aaatcaaaaa tcaaaatgca aaaaccaatt caaaatagga aacctttcga
     1261 atccgcttcc taattttgat ctttgatctt tgatttttga tttattttct cgattatttc
     1321 acggattccg agctcctctt cctcgcgggt cgcgagcgtc ttcagcttgt atttgccgct
     1381 tgaaacctcg ttctcgccaa taatgagcgc gtagggaacc gagtcctttt ccgctttctt
     1441 gagctggtcg ccgagcttct tgccggtaag gtcgactgcg gcggagagcc cggcgttccg
     1501 gagctctttg gcgagcgttt cggcgcgctc ggcggatgcg gaatcgacga cgcaaatata
     1561 gagttttgcc gaaggcgcat acgccggaag caggttcctt gattcgagaa aatcgcggag
     1621 cgtcacgtcg cccatgccga agccgaccgc cgggactttc tcgctgccga aaatcgagag
     1681 caggtcatcg tagcgtccgc cgccgaacat cgaacggcgg ttttccggat ccgtgtcgaa
     1741 gatctcgaaa atgattcccg tgtaatagtc gaagcctcgc atgagcgaga cgtcgaacga
     1801 ggtatttgcc acgccgaggc cgttcagccg ctcgatcacc tccatcgtct cgcggtagcc
     1861 ttcgcccgcc tttaccgctt cgatgtcgcc gttcgtcgaa aggagcgttt cgagctcggc
     1921 ggcgtattcg cccgcggctt cgcgaagctg taccgcgaat tcctccgcgg aaatcttgct
     1981 tttcttgtcg ataagcttcg agatcctata tgactgatcc ttgtcgaagc cgtagagctt
     2041 gtagatgtcg ttaagaattt ttcgggagct cactctgatc tcgaaatcgt cctgcttcgc
     2101 gccgaaattg gcaagaagct cggatgcgag ggcaatgact tcggtttcgg cccaggaatt
     2161 atccacgccg aaaatgtccg cattgagctg ccagtgctcg cggaggcgtc ccttttgagg
     2221 gcgctcgtag cggaagaggt tgggaatcga gaaccagcgg agcgggaagg agagttcgcg
     2281 tctgcgggcg gcaaccatgc gggcgacggt cggcgtcatc tcggggcgga gcgtcacttc
     2341 gcgctcaccg cggtcggtga acgtgtacgt ctgttcgttg acgatctctt ctccggactt
     2401 ggtcttgtaa agctcggcag gttccaggat cgacgcgtca tactccgagt agccgaagct
     2461 ccgcgccgtc tttctcatca ccccgaacat gtaatcctgg acgaatttgt cttccggata
     2521 gaaatcgcgg acgcccttgt agctttccgt agaaaccttt gattgatttt cttccatatc
     2581 gtttccatta tagtagccga tatgggtgtc gcgaacaagc gtctgcccgc tccttcaaca
     2641 cgcttcggac aggcaagata tgccgattcc aagccccaaa atcctggtga tttgacgagc
     2701 ttgccgttcg ccttgtattt cgtcgtaatt cgtgtataat atccgcaatc gtaagtttct
     2761 cataggcagt tcggccattg ggaaggtcgt taacgaggta attagaaaac aagaatatac
     2821 gacaaatggc aacgaagttg tatgttggca acatgtccta cagcacgacc gaagacggtc
     2881 ttcgcgaagc tttctcgaag gcaggaacgg tagtctctgc gactgttatc aacgacaaga
     2941 tcaccggccg cccgagagga ttcggattcg tcgaaatgga ggatgccgac gcacagaagg
     3001 caatcgacat gtggaacgaa aaggatctcg acggacgcaa ccttgtggtc aacgaggctc
     3061 gccctatgca gccgaggcag ggcggaatga ggtaaaagca tccgctcatc gaggactttg
     3121 tcctcgattc acaaaaggcg cgaagagcgc cttttgtgct gtccgcaacg ggcagcagca
     3181 cggatcttgt attgcaactt cactcgggtc gaatgaaaga agcctctcat tcaccgctcg
     3241 ttcgttgcaa taatttctca tccactctga cgcaaaaacc gcttcc
//
LOCUS       DASZYS010000050        11403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_569144, whole genome shotgun sequence.
ACCESSION   DASZYS010000050 DASZYS010000000
VERSION     DASZYS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569144"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..508
                     /locus_tag="VHF05_02995"
     CDS             182..508
                     /locus_tag="VHF05_02995"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVS79922.1"
                     /translation="MLKPNELKRLDKKVDTKKNERLAIIFNALSDESRCAMFRLFVSR
                     GREGLCVTDVAKLLKISPSAASQHLKILDITGLLTRKRKGQRVNYYASFDDPLVKAIT
                     EAVIRD"
     gene            530..1249
                     /locus_tag="VHF05_03000"
     CDS             530..1249
                     /locus_tag="VHF05_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVS79923.1"
                     /translation="MKYTTELISKETVAEGTMAFHMKKPEGFVFLPGQNCDFTIVNPP
                     ETDAEGNIRTFSITSSPSESEIAIATRMRDTAFKRTLKNAEPGLQIQFDGPMGSFTLH
                     EKASRPAIILVGGIGITPFMSIVKDAAERALPHKIYMFYSNRRPEDTAFLTVLQDLAK
                     KNPNFAFVPTMTEMEKSGGTWDGERGYIDMAMIDRHVSDKNDAIYYMAGPQGMVAAMR
                     KMLNDSGISNDDIKTEEFSGY"
     gene            1286..1855
                     /gene="folE"
                     /locus_tag="VHF05_03005"
     CDS             1286..1855
                     /gene="folE"
                     /locus_tag="VHF05_03005"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HVS79924.1"
                     /translation="MSAHYAAILELVGADTDSEGLRETPRRAAKALYEMTEGSRMGLE
                     RIVTLFKSECEATSCHDMVMVEDIREVALCEHHLLPFPMSITIAYIPGERILGLSKMM
                     RIAALFSRRLQNQERVGKQIADFMEEVLEPLGVAVLIEGRHTCSMARGVRDHNATMKV
                     DVMRGLFKEDPTIRAELLSRVGPRGKTLI"
     gene            1878..2336
                     /locus_tag="VHF05_03010"
     CDS             1878..2336
                     /locus_tag="VHF05_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HVS79925.1"
                     /translation="MKKYWLLKSEPSCYSIDDLAKDKTALWTGVRNFQARNFLRDMKV
                     GDLVLFYHSSEPPMGVFGVAKIAAEAVPDTTALDKEDDHYDPKATKENPIWFAPKVAF
                     SKKFKSPVTLSAIKFNPKLEGIMVAAQGSRLSVQPVSKEHFEEIVKMSST"
     gene            2412..2777
                     /locus_tag="VHF05_03015"
     CDS             2412..2777
                     /locus_tag="VHF05_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79926.1"
                     /translation="MITTKQAVYAEIIRHEAEDLWRMGFAFTIGIEGQPELMCLHRMN
                     LVGENYIESCLDSLADERGIPKEYFRFFIKENRLDAPVARPAPQACRIESKSYTGRNT
                     ICIMIIVALIMFVCRFAPV"
     gene            complement(2839..2985)
                     /locus_tag="VHF05_03020"
     CDS             complement(2839..2985)
                     /locus_tag="VHF05_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79927.1"
                     /translation="MASFGEWARRHNNESGEENEGSEKKKAEHSSDSDRDTAEPTAME
                     DEDK"
     gene            3130..4011
                     /locus_tag="VHF05_03025"
     CDS             3130..4011
                     /locus_tag="VHF05_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVS79928.1"
                     /translation="MNEFIIQTTGREEGYELIDSGAGEKLERYGEFAVARPDPQALWP
                     KRLSQAEWDKAHAYYRREGSKGKWIVKEGVPESWVAKIGEWSFRIAPSPFKHTGVFPE
                     QLSNWRWIGEKIASARERGREVRVLNLFGYTGGATLAAAKAGASVTHVDASKSSIGKA
                     RENASLSGLEAAPIKWILDDAKKYADREARRGSAYDAIILDPPTYGRGAKGELWEIEN
                     DLMPLLNSCLGLLSKDPLFVLMNGYASGYSALAYKNDLDYMMKNFGGTVEAGELAIKE
                     TSSGRLLPAGIFARWSR"
     gene            4458..4982
                     /locus_tag="VHF05_03030"
     CDS             4458..4982
                     /locus_tag="VHF05_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79929.1"
                     /translation="MQKKTRSFLIISASAVVILLMGIGLGRLIFGGNSSSPDLPEPTF
                     VIATSTSDGTAAESANSSAKSAQAPAPAADPLASCESSTKAEAASKGVGYDDSTILVS
                     FDTAVSYDDAVGILDSFGLAPHNSPSLKEQFDANHWLTVDVKKGDEFKEVCVVRGAEG
                     VKYAGPNYTFKLAE"
     gene            4984..5469
                     /gene="mscL"
                     /locus_tag="VHF05_03035"
     CDS             4984..5469
                     /gene="mscL"
                     /locus_tag="VHF05_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVS79930.1"
                     /translation="MKSELASTRGFIGEFKKFIARGSVVDLAVGIIIGGGFNTVVSSL
                     VNDIVMPPIGHIVGKVDFKDLYVNLSGTPYDSLTAAKAAGAATINYGNFLNNVISFLI
                     TAFAVFLLVKFINRLRDMERKKKQVEAPAPTTKDCPFCFTSIPARAVKCPACTSDLQA
                     K"
     gene            5530..6036
                     /locus_tag="VHF05_03040"
     CDS             5530..6036
                     /locus_tag="VHF05_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVS79931.1"
                     /translation="MKQHDTERERFLQAYDELSDSLFNHCFYRVSDREVALDITQETF
                     TKTWEYMAGGKDIGNLRPFLFRVAGNLIIDYYRKKKSDSLDALQEEGFDVPSEDVERI
                     ISSTEGKNALKALEGLDEKYRQVIVMRYIDELSPREIGEIIGETENAVSVRLNRSIKK
                     LKELLNLA"
     gene            6045..7304
                     /locus_tag="VHF05_03045"
     CDS             6045..7304
                     /locus_tag="VHF05_03045"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HVS79932.1"
                     /translation="MKKIEDIFNTLPKAVMTAEERTRIREAVSTFIAEHPVVSPLVAA
                     RISPFTKLLSLDFVQAHAFQALALILLVACGGITVAAQNALPGDPLYAVKLNVNEELP
                     GLFAFSTQAKADYEAGLAERRLNEVTSLSAEGKLTPEARTQIEANLQAHVDNVEKSVN
                     KLQSENDARGAVEVSSDLASTLHAHEQVLLDLSSRSDEAGEVASTLRQQLGSISDHLT
                     DLEEQVAGEETGDSEARARTKSEQAEDELSAAKSARADATTSVSEDVLDDADAQIDHA
                     LSELADGYTSLQNGSFNKAFVDFQSVIRYSKEAEVIIKAGVKYDLPADNDGDNASTTP
                     EEGSTTPSEATSTDSLGATTTNATGTPTTTDESASTSTGEAGTSATTSTSTLLLEGSF
                     SATSASPMPTQPGMLNKLLKAAQDVGL"
     gene            7360..8034
                     /locus_tag="VHF05_03050"
     CDS             7360..8034
                     /locus_tag="VHF05_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVS79933.1"
                     /translation="MHAKRNQIILAIILSGFVFAALAFLRLLPAAHSLDVFAAREALL
                     LRNPALTAFLATLTELFSIPVLLIVFAGMLAVLFFEPNKKPASFLLVSGLLSAALFAA
                     AKLMIHSARPAGALIAASGFGFPSGHAAVATTFFIFLGFLFEHVSRSRGVRLLFNLLS
                     ACMIVAVSLSRVILGVHWLSDVLAGMALGVFSSAVARLYFDPTEKFSKPESISKIFKK
                     SWKRAS"
     gene            8016..8876
                     /locus_tag="VHF05_03055"
     CDS             8016..8876
                     /locus_tag="VHF05_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HVS79934.1"
                     /translation="MEARVISGTEIRDTIKKDLTERTARLARKPALAIIQIGDNPESD
                     VYVKQKMRFGEEIGCEVTHILLAEGETEEGIIEEIRELNADERISGIIVQLPLPEKID
                     ADRALAAIDPAKDVDGLTPASLSRLLAGDLSGFIPATTKAVLKILEHAGQDAAGKRVT
                     VVGRSNLVGKPTALALLALDATVTVCHSKTEDLAEAAKQADILIVAAGKKHLIGREHV
                     AEGQIVIDVGITAETGEDGKRKLYGDVDFDAVKDIVAAITPVPGGVGPLTVASLFENV
                     VRAQEFAENK"
     gene            8964..9764
                     /locus_tag="VHF05_03060"
     CDS             8964..9764
                     /locus_tag="VHF05_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HVS79935.1"
                     /translation="MMKHPLARFVAVLCLVLAFLAAVETVKAFKEYRYIGYDATANNT
                     VVVHGEGEVFAPADIATFSFGVVTDGVTVAEAQSKETTQMKSVVAALMAEGIADADIK
                     TENYSVYPKYASDDSNSSAIVCTTAGYCPPQTRSQKIVGYEVSQSVSVKVRQSDKAGD
                     VVSKVGSMGVTNISGIDFTIDDQTKLQEEARQKAIADAKQKAAQLASDLGVSLGSIVG
                     YSENQSPIYFKAMDASVASGAAVAPAPATLPQGQNKVTSDVDITYKIR"
     gene            10054..10233
                     /gene="secE"
                     /locus_tag="VHF05_03065"
     CDS             10054..10233
                     /gene="secE"
                     /locus_tag="VHF05_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVS79936.1"
                     /translation="MSISDYIKDTRGELKHVSWPTRSQAIAYTVTVILIAIVIGIYLG
                     LLDYIFSNLLEKFVL"
     gene            10293..10835
                     /gene="nusG"
                     /locus_tag="VHF05_03070"
     CDS             10293..10835
                     /gene="nusG"
                     /locus_tag="VHF05_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HVS79937.1"
                     /translation="MSKQKIEGRNWYVIHTYAGYENAVMRNLKQRIESLGMEDKIFNV
                     IVPTEKKIKIKGGKRVEEEEKIYPGYIMVDMIVTDDSWYVVRNTPRVTGFVGSGVYPV
                     PLDPKEVDSLFKRMNSDKVTHKGDYHPDDTVMIVDGPFKDLEGRVSEVDDARGKIKVL
                     VSMFGRETPVELDFLQVRKA"
     gene            11005..>11403
                     /gene="rplK"
                     /locus_tag="VHF05_03075"
     CDS             11005..>11403
                     /gene="rplK"
                     /locus_tag="VHF05_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HVS79938.1"
                     /translation="MAKKIIKTLKLQAMGGAATPAPPLGPTLGQAGINIGEFVTKFNE
                     ATKEMRGEVVPAVVSVYEDRTFSFVLKTPPASQLILKAIGKDKGSGKAPSVKIGTITK
                     DQIRQIAEKKMPDLNANDVEAAMNIIAGTAR"
BASE COUNT         2664 a         3134 c         3181 g         2424 t
ORIGIN      
        1 caatgtacta ttcgtacatt ttggtcgcaa gcctcgcctg cttcgcggac tgcatccgaa
       61 tgagaaagcc ttggctggta tttctgagaa aaccccgcag ggtagtccgc ggggttttct
      121 aatttgttga cttctccagc cgcgcagagt atgatattga agtatttaag tatatattaa
      181 aatgcttaaa cctaacgaac tgaagcggct cgacaagaaa gtggacacca aaaagaacga
      241 gcggctcgcg atcattttca acgcgctgtc cgacgagagc aggtgcgcca tgttccggct
      301 cttcgtgtcg cggggaaggg aggggctctg cgtcacggat gtagcgaagc tcctcaaaat
      361 ttcgccgtcg gccgcttccc agcatctgaa gattctcgac atcacggggc ttctcacgcg
      421 gaagcgcaaa ggacaaaggg tgaattatta cgcgtccttc gatgatccgc ttgttaaggc
      481 gattaccgag gcagtcatta gagattgata atttaacgat tcatacgaga tgaaatacac
      541 aaccgaactt atttccaaag aaacagtcgc cgaaggcacg atggccttcc atatgaagaa
      601 gcccgaaggt ttcgtgtttt tgcccggcca gaactgcgat ttcacgattg tgaatccgcc
      661 ggagaccgac gccgaaggca acatcaggac attctcgatc acaagctcgc cctccgagag
      721 tgaaatcgcc atcgcgaccc gcatgcgcga tactgccttc aagcgcacgc tcaagaacgc
      781 ggagccgggc ctccagatac aattcgacgg gccgatgggc tccttcacgc tccacgagaa
      841 ggcgagccgt ccggcgataa ttcttgtcgg cggcatcggc atcacgccgt tcatgagcat
      901 cgtgaaggac gccgccgagc gcgcgctccc gcacaaaatt tatatgtttt actcgaaccg
      961 ccgccccgaa gataccgcat ttctcacggt cttgcaggat ctcgcgaaga agaatccgaa
     1021 tttcgcgttc gtgcccacaa tgaccgagat ggagaagtcc ggcggcacgt gggacggcga
     1081 gcgcggatat atcgatatgg caatgatcga ccggcatgtt tcggataaga acgatgcgat
     1141 ctattacatg gccggcccgc agggaatggt cgccgcaatg aggaagatgc tcaatgattc
     1201 cggcatatct aatgacgaca ttaagacaga ggagttttcg ggttattaaa atatggaaca
     1261 agatagagaa aaattaatcg acagtatgtc ggcgcactat gctgcgattc tcgagctcgt
     1321 cggcgccgat acggattccg aaggtctgcg cgaaacgcct cgacgtgcgg cgaaggcgct
     1381 ctacgagatg accgaaggca gccgcatggg gctcgaaagg atcgtaacgc tcttcaagtc
     1441 ggagtgcgag gcgacctcgt gccatgacat ggtgatggtg gaggatatcc gcgaagtcgc
     1501 cctctgcgag caccacctcc tgccgtttcc catgagcatc accattgcgt acattcccgg
     1561 cgagaggatt ctcggccttt cgaagatgat gcgcatcgcc gcgctcttct cgagacgcct
     1621 tcagaatcag gagcgcgtcg gcaagcagat cgccgatttc atggaagagg tgctggagcc
     1681 tctcggtgtc gcggtcttga tcgaaggcag gcatacctgc tcgatggcgc gcggcgtccg
     1741 cgaccacaac gcgacgatga aagtcgacgt catgcgcgga ctcttcaagg aggatccgac
     1801 aatccgcgcc gaactccttt cgcgcgtcgg accgcgcggg aagaccctca tataataagc
     1861 tctgctatac tacgaatatg aaaaaatact ggctgcttaa gagcgaaccg tcgtgctact
     1921 cgatcgacga tctcgcgaaa gacaagacgg cactctggac gggcgtgagg aatttccagg
     1981 cgaggaattt cctgcgcgac atgaaagtcg gcgacctcgt gctgttctat cattcgagcg
     2041 aaccgccgat gggagtcttc ggcgtggcga agattgctgc ggaagccgtg cccgacacga
     2101 cggctctgga taaggaagac gaccactacg atccgaaggc gacgaaggag aatccgatct
     2161 ggttcgctcc aaaagtcgcg ttctcgaaga aattcaaaag tccggtaacg ctcagcgcga
     2221 tcaagttcaa tccgaaactc gaggggatca tggtagccgc gcagggctcg aggctctccg
     2281 tccagcctgt atcgaaggag catttcgagg aaatcgtgaa gatgtcctct acgtaaaacg
     2341 gagcgggcat tcttgaccat ttttaataac catgcttaaa ttattgcaga tcgatctcac
     2401 aaggagagga aatgattacg acaaagcagg cagtatatgc cgagattatc cggcatgagg
     2461 cagaggattt atggagaatg ggattcgcgt tcaccatcgg catcgaagga cagccggaac
     2521 tcatgtgcct gcacaggatg aaccttgtgg gcgaaaatta tatcgaaagc tgtctcgaca
     2581 gccttgccga tgaacgtggg ataccgaagg agtattttag gtttttcatt aaggaaaatc
     2641 gcctggatgc acccgtggcg cgtcctgcgc ctcaagcgtg cagaattgaa tcaaaatctt
     2701 acacagggcg aaatacgata tgtatcatga taatcgtcgc cttaataatg tttgtatgcc
     2761 gctttgcccc agtttgattg ggcagcaaag caaggccgcg cgtcgtagac gcgcggcctc
     2821 ttgttatcta ttcgattgtt atttgtcctc gtcttccatt gcggtaggtt ctgctgtatc
     2881 gcgatcggaa tcggacgagt gttccgcctt ctttttttcc gatccctcat tttcttcgcc
     2941 ggattcgttg ttgtgccgtc gtgcccattc tccgaaactt gccatttttg ttgtggagtt
     3001 acgcggttaa gatcgaccct ggcaccagtt tactctcgga aggtgaatga gccgtgaatc
     3061 gcttcataat tcgcctctcg cgcgaggaga gcggtttact cggggcgcgt gtcatgcaag
     3121 tataaggcta tgaatgaatt catcatccag acgacggggc gggaagaggg ctacgagctc
     3181 atcgattcgg gagcgggcga gaagctcgag cgctacggcg agtttgccgt cgcccgtccc
     3241 gatccgcagg ccctgtggcc caagcggctc tcgcaggcgg aatgggacaa ggcgcatgcg
     3301 tactatcgcc gcgagggctc aaagggaaaa tggatcgtga aggagggcgt gccggaatca
     3361 tgggtagcga agatagggga atggagcttc cgaattgcgc cgtcgccctt caagcatacg
     3421 ggcgtatttc ccgaacagct ttccaactgg cggtggatcg gcgaaaagat cgcgtccgca
     3481 agagagcggg gccgcgaagt tcgggtgctc aatcttttcg gatataccgg cggcgcgacg
     3541 cttgcggcgg cgaaggcggg cgcgtcggtg acgcatgtcg acgcgtcgaa atcgtcgatc
     3601 ggcaaggcga gggaaaacgc gagcctctcc ggcctcgaag ccgcgccgat aaaatggata
     3661 ctcgatgatg cgaaaaaata cgccgaccgc gaagcgcggc gcgggagcgc atatgacgcg
     3721 attattttgg atccgccgac ctacggccgc ggcgctaagg gggagctgtg ggaaattgag
     3781 aatgacctca tgccgctttt gaattcctgt ctcgggctcc tctcgaaaga tccgcttttt
     3841 gttttgatga acggctacgc ttccggctac tccgcgctcg cctacaagaa cgatctcgac
     3901 tacatgatga aaaatttcgg gggaacggtc gaagcggggg agcttgcgat caaagaaaca
     3961 tcttccggcc gcctcctgcc tgccggcatt ttcgctcgct ggtcgcgcta atgcgaattt
     4021 gaatataaaa ataacccgcc ggcctctcgg ttgagaggcg ggcggatttg ttttgcggcg
     4081 aagtttaacg ttcgccatcg aagtacgatc ctggtacggt gccgtaccgt aggcgtacgt
     4141 ctttaagttc gaaaacgcaa tggacaatga tattgtcgcc gtcttgctcc tgttggtgta
     4201 tgacggctgc gccgatgacc tggtggtgag gtttctcggt aaggtcggta agtgactcaa
     4261 gtactaatcg tgcattgcac gatgttactt tgtcgattga tgtattggaa agttgtggat
     4321 ctacggggat acctatcacg aattgtgctc ctcttctgct attgtgctcc tccggtattt
     4381 attgtcaagc tgagaaagat cccggacgtg catagcggcc tcgcggggcc cgcgacttct
     4441 gatatcatta tcactatatg caaaagaaaa cgagatcttt tcttattatt agcgcctcgg
     4501 cggtcgtaat ccttcttatg ggcatcgggc tcggaaggct tatcttcggc gggaactctt
     4561 cttctcccga cctgccggag ccgaccttcg taatcgccac gtcgacttcg gacggaactg
     4621 ccgcagaaag cgccaattct tcggcgaaat cggctcaggc tccggcacct gccgccgatc
     4681 cgctcgcgtc gtgcgagtct tcgaccaagg cggaagcggc gagcaagggc gtgggctacg
     4741 acgacagcac cattctcgtt tccttcgaca cggcggttag ctacgacgac gctgtcggaa
     4801 ttcttgactc gttcggcctc gccccgcata atagcccaag tctcaaagaa caattcgatg
     4861 ccaatcattg gctcacggtc gatgtgaaga agggcgacga gttcaaggaa gtgtgcgtcg
     4921 tgcgcggcgc ggaaggcgtg aagtatgctg gacccaacta tacgttcaaa cttgcagaat
     4981 aaaatgaaaa gcgaactcgc atcgacacgg ggcttcatcg gcgaattcaa gaagttcatc
     5041 gcgcgcggca gcgtcgtcga tctcgcggtc ggcatcatta tcggcggcgg attcaacacg
     5101 gtcgtctcgt ctttggtgaa cgacatcgtc atgccgccga tcggccacat tgtcggcaag
     5161 gtcgatttca aggatctcta cgtcaatctg agcggcacgc cgtacgattc gcttaccgcg
     5221 gcgaaagcgg cgggcgcggc gaccatcaat tacggaaatt tcctgaacaa cgtcatcagc
     5281 ttcctcatca ccgcgttcgc agtctttctc ctcgtcaaat tcatcaaccg gctccgcgat
     5341 atggaacgga aaaagaagca ggtagaggcg cctgccccta ccacgaagga ctgcccgttt
     5401 tgctttacga gcattccggc gcgcgccgtg aaatgccccg cctgcacctc agatctccag
     5461 gcaaaataag gcgatttccg cagggaccgg aaatcgtgta attttgacat agtctctccg
     5521 tcattacata tgaagcagca cgatacagaa cgggaaaggt ttctccaggc gtacgacgaa
     5581 ttgtccgatt cgcttttcaa ccattgcttc tacagggtct ccgaccgtga agtggccctc
     5641 gacataaccc aggaaacttt cacgaaaacc tgggagtaca tggcgggagg gaaggatatc
     5701 ggaaatctcc ggccgttcct cttcagggtg gcgggaaacc tcatcatcga ttattaccgg
     5761 aagaaaaaaa gcgattcgct cgacgcgctg caggaagagg gctttgacgt tccgtcggaa
     5821 gacgtcgagc ggataatctc ctcgaccgaa ggcaagaacg cgctcaaggc gctcgaaggg
     5881 ctcgacgaga aataccgcca ggtgatcgtc atgcgataca tcgacgagct ctctccgcgc
     5941 gaaatcgggg agatcatcgg agaaacggaa aatgccgtgt ctgtccgcct caatcgaagc
     6001 atcaagaaac tcaaggaatt gctcaatctc gcctgaaacc aataatgaaa aagatcgaag
     6061 acatatttaa tacgcttccg aaggctgtga tgacggcaga ggaaaggacg cgcatccgcg
     6121 aggcggtttc gacgttcatt gcggagcacc ccgtcgtctc tcctctcgtt gccgcccgca
     6181 tctctccgtt caccaagctt ctttcgctcg atttcgtgca ggcgcacgcc ttccaggcgc
     6241 tcgcgctcat ccttctcgtc gcctgcggcg gcattaccgt cgcggcgcag aacgccctcc
     6301 ccggcgatcc tctctatgcc gtgaagctca acgtcaacga ggagcttccg ggactcttcg
     6361 cgttctcgac gcaggccaag gccgactacg aggccggtct cgccgagcgc cgcttgaacg
     6421 aagtaacgag cctctccgca gaaggcaagc tcactcccga agcccgcacc cagatcgaag
     6481 ccaatctcca ggcgcatgtc gacaacgtcg agaagagcgt caacaagctt cagtcggaga
     6541 acgacgcccg cggcgccgtc gaggtgagct ccgatctcgc atcgaccctt catgcgcacg
     6601 agcaggtcct tctcgacctt tcgagccgct cagacgaggc aggcgaagtc gcctcgacgc
     6661 tccgccagca gctcggcagc atctccgatc acctgaccga tctcgaggag caggtggcgg
     6721 gcgaagagac gggcgactcc gaagcgcgcg cccgcaccaa gtccgagcag gccgaggatg
     6781 aactctccgc ggcaaagtcc gcgcgcgccg acgccaccac ttccgtgagc gaggatgtgc
     6841 tcgatgacgc ggatgcgcag atcgaccatg cactgagcga acttgccgac ggctacacca
     6901 gcctccagaa cggatccttc aacaaggcct tcgtcgattt ccagtcggtc atccgctaca
     6961 gcaaggaggc ggaagtgatc atcaaggcgg gcgtgaagta cgatctgcct gccgacaacg
     7021 acggcgacaa cgcatccacc acgccggaag agggaagcac cactccgtcg gaggcgacct
     7081 cgaccgattc actcggcgct acgactacga acgccacggg aactccgacc accacggatg
     7141 aatcagcctc aacttcgaca ggcgaagccg gcacctcggc aaccacctcg acctcgacgc
     7201 ttctcctgga gggttcgttc tccgcgacat cggcttcgcc catgccgacc cagcccggaa
     7261 tgctcaacaa gcttctcaag gcggcacaag acgtcggcct ctaatttttt accaaaagcc
     7321 cgcagcagag ccgcgggctt tttgctatat aattgcggta tgcatgcgaa gcggaatcag
     7381 attattctcg cgatcattct ttcgggattc gttttcgccg ccctcgcttt tctccgtctt
     7441 cttcccgcgg cgcattccct cgatgtgttc gccgctcggg aagctctgct tctccgcaat
     7501 ccagcgctta ccgcttttct cgccacgctc accgagcttt tcagcattcc ggtccttctc
     7561 atcgtgttcg ccggaatgct cgcggtgctt ttcttcgagc cgaacaagaa gcctgccagc
     7621 ttccttctcg tgtcggggct tttgagcgcc gcgcttttcg ccgccgcgaa gcttatgatt
     7681 cattcggcgc gccctgctgg ggcgcttatc gccgcctcag gcttcggttt tccgagcggc
     7741 catgccgctg ttgccacgac cttcttcatt tttctgggat ttctcttcga gcacgtatca
     7801 cggagtcgcg gcgtgcggct gcttttcaat cttctgtcgg catgtatgat agtcgctgtg
     7861 agcctgagcc gggtcatcct cggcgtccac tggctctccg acgtcttggc gggcatggcg
     7921 ctcggagtgt tctcgtcggc ggtcgcgagg ctgtacttcg acccgacgga gaaattttca
     7981 aaaccagaat ccatctcaaa aatatttaaa aaatcatgga agcgcgcgtc ataagcggga
     8041 cggaaatccg cgacacaata aaaaaggatc tcacggagcg gaccgccagg cttgcgcgca
     8101 agcctgcgct cgcgattatc cagatcgggg acaatcccga gtccgacgtc tatgtgaagc
     8161 agaagatgcg ttttggcgaa gaaatcggct gcgaagtgac gcatattctg cttgccgaag
     8221 gcgagacgga ggaggggatt atcgaagaga tacgagaact taatgcagac gaacgcatct
     8281 cgggcattat cgtccagctt ccgcttcccg aaaagatcga cgcggacagg gcgcttgccg
     8341 cgatcgatcc ggcaaaggat gtcgacggcc tcacgcccgc aagcctctcg cgccttcttg
     8401 cgggcgatct ctccggattc attcccgcga ccaccaaggc agtgcttaaa attctcgagc
     8461 atgcggggca ggatgcggca gggaagaggg tgactgtcgt aggccgctcg aacctcgtgg
     8521 gaaagcccac cgcgctcgcg ctcctcgcgc tcgatgccac cgtgacggtc tgccacagca
     8581 agacagagga tctcgcggaa gctgcgaagc aggcggatat tctcattgtc gccgcaggaa
     8641 agaagcatct gatcggccgt gaacatgtcg cggaagggca gattgtcatc gatgtgggca
     8701 ttaccgccga aaccggcgaa gatgggaagc ggaagctcta cggcgacgtg gatttcgatg
     8761 cggtgaagga tatcgtcgcc gcaattactc ccgtacccgg cggcgtgggg cctctcacgg
     8821 tcgcgtcgct ttttgaaaat gtcgtgcgcg cgcaggaatt cgcagaaaat aaatgaacga
     8881 tgtggattag ccatgcgccg ctgtcgggta taattgagag cgagtttaga atttagtgca
     8941 aaataatgga aatgagttca aatatgatga aacatcctct cgcgcgcttt gtcgcggtgc
     9001 tctgcctcgt gctcgcgttc ctcgcggccg tcgagacggt caaggcgttt aaggaatatc
     9061 gctacatcgg ctatgatgcg acggcgaaca atacggtcgt cgtccacggc gaaggcgaag
     9121 tattcgcgcc ggccgacatt gcgaccttca gcttcggcgt cgtgactgac ggcgtcaccg
     9181 tggcggaggc gcaaagcaag gagacgacgc agatgaagag cgtcgtcgcg gcgctcatgg
     9241 cggagggcat cgcggatgcc gatatcaaga cggagaatta ctcggtatat ccgaagtacg
     9301 cgagcgacga ttccaacagc tcggcgatcg tctgcaccac tgcaggctac tgccctccgc
     9361 agacgcgctc ccaaaaaata gtcggctatg aagtgagcca gtcggtgagc gtcaaggtgc
     9421 gccagagcga caaggccggc gatgtcgtat cgaaggtcgg ctccatgggc gtgacgaaca
     9481 taagcggcat cgacttcacg atcgacgacc agacgaagct tcaggaagag gctcgccaga
     9541 aagcgatcgc cgatgccaag caaaaagcgg cacagctcgc gagcgacctc ggcgtaagcc
     9601 tcggctcgat cgtcggctac agcgagaatc agagcccgat ctacttcaag gcgatggatg
     9661 cgagcgtcgc ctccggcgcg gcggttgctc ccgctccggc aacccttccg cagggccaga
     9721 acaaggtgac ctcggacgtg gatatcacct ataagatcag ataacgctgg caacaatgta
     9781 ttcggcaaca tcgcatggat atcgtgcgat gttttcgata tgtaaattct caatttgcaa
     9841 tttgcaattt tcaatcaaat cacaatgaat caattcccgc ctgccggcag gcaggttcaa
     9901 aggtttgaat cattgaaaat tcaatgaaaa attggaaatt gagaattgaa aattatgccc
     9961 aatcaattca tttgacttat tttattcagt cgagtattat aaatggtgcc cacgggggcg
    10021 ttttttagta cgtcggtttt caccatctat tccatgagca tcagcgacta catcaaggat
    10081 acgcgcggcg agctgaagca tgtcagctgg ccgacccgtt cgcaggcgat tgcctatacc
    10141 gtcacggtca tcctgatcgc gatcgttatc ggcatttatc tcggtctctt agactacatc
    10201 ttttcgaacc tcctcgagaa gttcgtactt tagtaatcgc gaatagccgc gaatcggttc
    10261 gcgtcaatat tcgggattga taagcaaaaa tcatgagcaa acaaaaaatc gaaggaagga
    10321 actggtacgt catccatacc tatgccggct acgagaacgc cgtcatgagg aacttgaagc
    10381 agcgcatcga gtctctcggc atggaggaca agatcttcaa cgtcatcgtt ccgaccgaaa
    10441 agaaaatcaa gatcaagggc ggcaagcgcg tcgaggaaga ggaaaaaatc taccccggct
    10501 atatcatggt cgacatgatc gtgaccgacg actcgtggta cgtcgtccgc aatactccgc
    10561 gcgtgaccgg cttcgtcggc tcgggagtgt atccggtgcc cctcgatccc aaggaggtcg
    10621 actcgctctt caagaggatg aactccgaca aggtgaccca taagggcgat tatcatcctg
    10681 acgataccgt catgatcgtc gacggcccgt tcaaggatct cgaaggaagg gtttccgaag
    10741 tggacgacgc gagagggaag atcaaggtgc tcgtctcgat gttcggccgc gagaccccgg
    10801 tcgagttgga ttttctccag gtgagaaagg cctagctata agtcaaaagg caaaattcaa
    10861 aaatcaaaat tataattcaa aatcctttaa ctttccgaaa tttttgattt gaacctttgc
    10921 cttttaactt ttgacttttg aattagttta cgtattataa acacgctgct tctaagcctt
    10981 tggctactaa ccaaagcaca tacaatggca aagaaaatca tcaaaacatt gaagctccag
    11041 gccatgggag gcgccgcaac tccggctcct ccgctcggcc ctaccttggg ccaggccggc
    11101 atcaacatcg gcgagttcgt gaccaagttc aatgaggcca cgaaggagat gaggggagag
    11161 gtcgttccgg cggtcgtttc cgtctacgag gacaggacct tcagcttcgt cctgaagacc
    11221 cctccggctt cccagcttat tttgaaggct atcggcaagg acaagggatc gggaaaggcg
    11281 ccgtccgtca agatcggtac tatcaccaag gatcagatcc gccagatcgc cgagaaaaag
    11341 atgcctgacc ttaatgccaa cgacgtcgag gccgcgatga acatcatcgc cggcaccgcc
    11401 agg
//
LOCUS       DASZYS010000051         7827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1080777, whole genome shotgun sequence.
ACCESSION   DASZYS010000051 DASZYS010000000
VERSION     DASZYS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080777"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VHF05_03080"
     CDS             complement(<1..1313)
                     /locus_tag="VHF05_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79939.1"
                     /translation="MTYEDITKSAREFRPALRFDAAISHAARAAVRRILYAVAILSIL
                     LSLEIFTEHTAGFLSVFFLSLSALGVLIGLEIFYSSIYYMRLPDGMSFELARVVSDTN
                     QSDITGGFLGSRFGRDLAKRLGLAESEIGNFLSSRSGRVAASDFVFAEGASLSLASYA
                     RAVFESDKELKDRFFGDGLLEDDFTGAALWLERNLVRWKEAREWWSRRNLSKVQGLGK
                     ALSYGQVWLLKKYASEFAAAHDLSGIDAGGGYHAKEADETELVLSRSSEANALLVAED
                     TSGALDVVERLAKRLANGSIHPALEHKQLFELDTNALIAATGNKNAFELTLMKLMQES
                     IKAGNIVLVIPDLVSFMSSAATLGSDVASVIDPYISSSAIQIIALADVGGFHQSIETN
                     NALMQRFERVSVKGGTNEETIRFLEDRIEALERRNGVFFTYQALTA"
     gene            complement(1315..1677)
                     /locus_tag="VHF05_03085"
     CDS             complement(1315..1677)
                     /locus_tag="VHF05_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79940.1"
                     /translation="MWHYGRSIRGYFSVWGNFFWFAYNFFSIPLLLRTLFSPFERLSE
                     EYRKGLDLSALASTLLVNLIMRLVGAAVRGVIIIFGLGALCFVAAAGLLLLIAWILLP
                     FIVFAFFLLGVHGLIEWI"
     gene            complement(1800..2669)
                     /locus_tag="VHF05_03090"
     CDS             complement(1800..2669)
                     /locus_tag="VHF05_03090"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HVS79941.1"
                     /translation="MRSFRHSTLASPKLRKRKRKVFLMRGIIIFVLLILLVVGISMLS
                     RMQSVTIRDVTVSGNSITATDDIVAAVEGKLDGNDWLLFPKKNFLIYPKRAIEESLRS
                     QFPALRSVSVALADPHTLGVRVTERTPQALWCRYDGTLPQPLTDNCYLMDAAGFVYLK
                     APEFKGNAYMRYYGLIDPAEPVGQNDLPDSIAKISAFVDGVRDLGLSPSAISVLNGEY
                     DLYLADGGRIIFDPDADYNDTLLNLATLIQNNATESGSSSSIDYIDLRYGNKVYYKFK
                     DGSGNTPQDGVSR"
     gene            complement(2805..4073)
                     /gene="serS"
                     /locus_tag="VHF05_03095"
     CDS             complement(2805..4073)
                     /gene="serS"
                     /locus_tag="VHF05_03095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVS79942.1"
                     /translation="MLDIKFIRENKDLVAMAAKKKLIKFDVDELIKADDKRREKLAAV
                     EAKRAEQNAASEKIAKVETPQERLYMIEQMKAVKESLQKEEEELKEIMKEWQGLMVQV
                     PNIPDVSVPEGEDDGANQEIRVWGEIPKFKFQPKSHTELMTALDMADFSRGTKVAGFR
                     GYFLKNDGARLAFALWQYVLDMYVEKGFTPMVVPSLVRREPFMGTGYLPQSEEDLYKT
                     QDNDYLSGTGEVATMGYFMDETLEKAQLPMKFISFSPCFRREAGSHGKDTQGLMRVHE
                     FFKLEQVVLCEADHATSVVFHEELTQNAEQMLQNLGLPYHVVVNCGGDLGLGQVKKYD
                     IETWVPSENKYRETHSSSYFHDFQTRRLNIRYRDADGKLKFAHSLNNTALASPRILIP
                     LIENNQNEDGSIRIPEPLQRYMGGRETIFR"
     gene            complement(4145..>7827)
                     /locus_tag="VHF05_03100"
     CDS             complement(4145..>7827)
                     /locus_tag="VHF05_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HVS79943.1"
                     /translation="LFDNRLSATSTLPNITTLANLAAVGTIANGVWQGTVIGSSYGGA
                     GTVNGILKANGSGVVSAAVAGTDYENPLAFSYPLARSTNIVSLAFGTTTANSWSQLQR
                     FNGGASTTLFTATGATYLATAGGNVGIGTTSPSSALSVNGNALLAGALTATNIISTSA
                     ATSTFGGGVNLTSGCFAVSGTCIGSGSNPWLSGGSNIYFTTGNVGIGTTSPQENLSVS
                     GSGGTWISVDNGGVSGNVGLQLKNSGAADWFVYMPSGTDDFRLSNQSQGWGDVFTVKA
                     NGSVGIGTTSPSSKLSVVGGLYATASSTMAGINLTNGCFAVNGTCITSGGGGSGTVNS
                     GTTGQIAVYASNGTTLSATSSISGLNLNFLQAGTSSVSRTLDSKLKDRVSVKDFGAKG
                     DGVTDDTAAIQAALNASLNVYFPPPAVDYRTTSALVLRSGQTVYGPATIYVESNTDGL
                     YASSSVSGTRNIVIKDLFIVDEYGGTRTAGYGVHIDGALDAAIVDIEGVRTYGFIDGM
                     NLSGLMQSDISFSRMDVASRNGMTIGGQSTSVTLHSTYADAPGQDCYSADGLNYSTFI
                     STACDDAGRDGYHITSDTFDATAVSFESAGAETVGRHGMYLDGWGFTSNSFTVSGVGT
                     GSGQAGIYVAGGHHMSFNNTRVTGGDYGLYDVASSDVSDTNPRYDNPAVAEVGDPNNI
                     VGSSFMGSINIGTTTDPVGKLHVAADSEDYFQSPLVTISNNSSSALLDLDRGNEFRMS
                     GIQFTTAGSNDWFAGTYYGDGNNQTGFGIGTDYQSSNQKLVVTAAGDVGIGTTSPAYK
                     LDVAGGLHTTSTSTMAGINLTSGCFAISGSCIGTGSGSSPWLTSGSNVYFSTGAVGIG
                     TSTPAENLSVVGSGGTWVSIDDDNTSGNTGFKFKNSGADKWFMYTPGNSDDFRLNNQS
                     QGWGDVFTVKANGDVGIGTTSPTTALQVAGTITPNTDNSGTLGNTTYRWNAVYATNGT
                     IQTSDERLKKNIETLDATSSFAALLALKPVSFNWIDPLAGTDTHLGFIAQDVQLVLPQ
                     VVSVGTDPDHTLGLRYTEFIPVLVKGFQDVANVAGGFKDNLIAWLGSATNGIKDLYAS
                     VFHAQEVDTDKLCVGGTCVTEAEFAHLVEQSGASSGGSETLAPEPPPQSEIGDGTTTD
                     DTATTSDETSDGSTTPAEDMEDTSVTSPEESSSSPATEDDGQAVSPDETDDGASDTSA
                     ENTPPAPQPEPQSDDGGEAPAP"
BASE COUNT         1646 a         2250 c         2289 g         1642 t
ORIGIN      
        1 gccgtgagcg cctggtacgt gaagaatacg ccgttccgcc tttcgagcgc ctcgatcctg
       61 tcttcgagga accggatggt ctcctcgttc gtgccgccct tcaccgatac gcgctcgaag
      121 cgctgcatga gggcattgtt cgtttcgatg gactgatgga agccgccgac atctgcaagc
      181 gcgatgatct ggatcgccga ggacgagatg tacgggtcga tgacggaagc gacgtcgctt
      241 ccgagggtcg ccgcgctcga catgaacgac acgagatcgg gaatgacgag cacgatattg
      301 ccggccttga tggattcctg catgagcttc atgagggtca gctcgaacgc attcttgttc
      361 cccgtcgccg cgataagcgc gttcgtatcg agctcgaaaa gctgtttgtg ctcgagagcg
      421 ggatggatcg atccgttcgc aaggcgcttg gcgagccgct ccaccacgtc gagcgccccc
      481 gatgtgtctt cggctacgag gagcgcgttg gcctcgctcg agcgcgacaa tacgagctcg
      541 gtctcgtccg cttccttcgc atgatagccg cctccggcgt cgatgcccga aaggtcgtgt
      601 gccgcggcga attcactcgc gtatttcttg agaagccaga cctgcccgta ggagagcgcc
      661 ttgccgagcc cctggacttt cgagagattg cgccggctcc accattcgcg cgcctccttc
      721 cacctcacga gattgcgctc aagccagagc gccgcgcccg taaaatcgtc ctcaagcagg
      781 ccgtccccga aaaagcggtc cttgagctcc ttgtcgctct caaagacggc tctggcatac
      841 gatgcgaggc tgagcgacgc accttcggcg aaaacgaaat cgctcgcggc gacccgtccg
      901 gagcgggacg aaaggaaatt tccgatctcg ctttccgcga gccccagtct cttcgccaga
      961 tccctgccga aacgcgaacc gagaaaacct ccggtgatat ccgattgatt cgtatccgaa
     1021 acgacccttg cgagctcgaa cgacatgccg tccggaagcc gcatgtagta tatggaagaa
     1081 tagaagattt cgaggccgat cagcacgccg agggcggaaa gcgagaggaa gaagacggaa
     1141 aggaatccgg ccgtatgctc ggtgaaaatc tcaagcgaaa ggaggatcga aaggatggcg
     1201 accgcgtaca ggattctccg cacggcggcc ctggcggcgt gcgaaatcgc ggcatcgaac
     1261 ctgagtgcgg ggcggaattc ccgggcgctt tttgtaatat cttcgtaggt catatcatat
     1321 ccattcgata agcccgtgca cgccgagcag gaagaacgcg aagacgatga acggcagcaa
     1381 aatccaggcg atgagaagaa gaagccccgc cgccgcgacg aagcagagcg cgccaaggcc
     1441 gaaaatgatt atcacgccgc gcacggcggc gccgacaagc cgcatgatga ggttcaccag
     1501 aagggtcgac gcgagagcgg aaagatcgag gcctttccta tattcctcgg aaagccgttc
     1561 gaagggcgaa aaaagcgttc tgagaagaag cgggatcgaa aagaagttat aggcgaacca
     1621 gaaaaaattt ccccagacgg agaaatagcc ccgaatgctc cggccgtaat gccacatggc
     1681 atactcgctc aaaaaaagag gaacgtttat gaacatcgct tggcctgaat cgcgaatgaa
     1741 gcggcagttc ccttccgctc cgcccgcgtt tcgttctatc tattgtagct caaacgcggc
     1801 tagcgcgaca cgccgtcctg tggagtgttg ccgctcccgt ccttgaattt gtagtagacc
     1861 ttgttgccgt agcggaggtc gatatagtcg atcgaggagg aagaacccga ctcggtcgca
     1921 ttgttttgaa taagggtcgc gagattgaga agcgtgtcgt tgtaatctgc atcaggatcg
     1981 aaaataatcc tgcctccgtc ggccagatag agatcgtatt cgccgttcaa aaccgaaatc
     2041 gcggacggcg agagccccaa atcgcgcacg ccgtcgacaa atgcggagat tttcgcgatc
     2101 gaatccggca gatcgttctg ccctaccggc tctgccgggt cgatcaggcc gtaatagcgc
     2161 atgtatgcgt tccccttgaa ttcgggagcc ttgagataga cgaagcccgc ggcatccatg
     2221 agatagcaat tgtccgtaag cggctgcggc aacgtgccgt cgtagcggca ccagagagcc
     2281 tgcggagtcc gttcggtcac gcgcaccccg agggtatggg gatcggcaag cgcgacggaa
     2341 acgctgcgga gcgccggaaa ctgcgaacgg agcgattctt cgatcgcccg tttcggatat
     2401 atcaaaaaat ttttcttcgg gaagaggagc cagtcattgc cgtcgagctt gccttccact
     2461 gcggcgacaa tatcgtccgt agcggtgatg gaattgcccg acaccgtcac gtcccggatc
     2521 gtcaccgact gcatcctgga gagcatggag ataccgacga caaggagaat aaggaggaca
     2581 aaaataatga tgccgcgcat gagaaagacc ttgcgcttcc ttttccgaag cttgggcgag
     2641 gccagcgttg agtgtctgaa agatctcatt ttaatgattt gtatactgac ctcagaattc
     2701 agcgcactcc tgcgttgctc gtaaaatttg atcccgcaga gtattgctca tacactttgg
     2761 tctcaaattt tactcacgcc ttggattgca tctgaattcc gaggtcatcg aaagatggtt
     2821 tctcttccgc ccatgtatcg ctgcagcggc tcgggaatgc gaatcgatcc gtcctcgttc
     2881 tgattgttct cgatgagcgg aataaggatg cgcggcgatg cgagcgcggt gttgttcaag
     2941 ctgtgcgcga acttgagctt gccgtccgcg tcgcggtagc ggatgttgag acgccgcgtc
     3001 tggaaatcat ggaaatatga ggatgaatgc gtctcgcggt acttgttctc cgatgggacc
     3061 caggtctcaa tatcgtattt tttgacctgg ccgaggccga gatcgccgcc gcaattcacg
     3121 acgacgtggt atggaaggcc taggttttga agcatctgct cggcattctg cgtgagctcc
     3181 tcatggaaca cgaccgaggt cgcgtggtcg gcctcgcaca gcacgacctg ctcgagcttg
     3241 aagaattcat ggacgcgcat gaggccttgg gtatccttgc cgtggcttcc cgcttcgcgg
     3301 cggaaacacg gagagaacga aatgaatttc atgggcagct gcgccttttc gagcgtctcg
     3361 tccatgaagt atcccatggt cgccacttcg ccggtgccgg aaaggtaatc attgtcctgc
     3421 gtcttgtaaa gatcctcctc gctctgcggc agatatcccg tgcccatgaa cggctcgcgc
     3481 cggacgagcg acggaacgac catcggcgtg aagcccttct cgacgtacat gtctaaaaca
     3541 tattgccaga gcgcgaacgc gaggcgcgcc ccgtcattct tcaagaagta gccgcggaag
     3601 cccgcaacct tcgtgccgcg cgagaagtcc gccatgtcga gcgcggtcat gagctcggtg
     3661 tggctcttcg gctggaattt gaatttcgga atttcgcccc aaacgcggat ctcctggttc
     3721 gcaccatcat cctcgccttc cggcacggac acgtcgggaa tgttcggaac ctgcaccatg
     3781 aggccctgcc actccttcat tatttccttg agctcctctt cctccttctg gagcgattcc
     3841 ttcacggctt tcatctgctc gatcatatag aggcgctcct gcggcgtctc gaccttcgcg
     3901 atcttctcgc tcgccgcatt ctgctccgcg cgcttcgcct cgaccgccgc gagcttctcg
     3961 cggcgcttgt cgtcggcctt gataagctca tcgacgtcga acttgatgag cttcttcttc
     4021 gcggccatcg ccacgagatc cttattttcc ctgatgaatt tgatgtcgag catggacgaa
     4081 aatacctaga tactggattt ttgaatgata acagaacttc gggggcagtg ctaccgcgag
     4141 ccggctacgg cgcaggcgct tccccgccat cgtcggattg aggctcgggc tgaggcgccg
     4201 gaggagtatt ttccgcagag gtatcggaag cgccatcatc cgtttcatca ggcgaaacgg
     4261 cttggccgtc gtcttccgtt gccggagaac tggaagattc ctcgggggac gttaccgacg
     4321 tgtcttccat atcttcggca ggcgtagtgc tgccgtcgga agtttcatca gaagtcgtgg
     4381 ccgtatcgtc cgtcgtagta ccgtcgccaa tttccgattg cggcggaggc tccggagcga
     4441 gagtctccga tccgccggac gaagcccccg attgctccac gagatgtgcg aactccgctt
     4501 ccgtcacgca cgttcctccg acgcagagct tgtcggtatc gacttcctgc gcatgaaaga
     4561 cgctcgcata aagatccttg atgccgttgg tcgccgagcc gagccatgcg atcaaattgt
     4621 ccttgaagcc tccggccacg ttcgccacgt cctggaagcc cttcaccagc accggaataa
     4681 attccgtgta gcggaggccc aaggtgtgat cgggatcggt gccgaccgag acgacctgcg
     4741 gcaagaccaa ttgcacatcc tgcgcgatga atccgagatg cgtgtcggtg ccggcgagcg
     4801 gatcgatcca gttgaaggaa acgggcttca gggcgagaag cgccgcgaac gacgaggtcg
     4861 catcgagggt ctcgatattt ttcttcaaac gctcgtcgga cgtctggatc gtgccgttcg
     4921 tcgcgtacac cgcgttccat cgatatgtcg tattgccgag cgtgcctgaa ttgtcagtgt
     4981 tcggcgtgat cgtgccggcg acctggagcg cagtcgtggg cgacgtcgtg ccgatgccga
     5041 catcgccgtt cgccttcacc gtgaagacgt cgccccagcc ctgggactgg ttgttcagcc
     5101 tgaagtcgtc agagttgccg ggcgtgtaca taaaccattt gtctgctccg gaattcttga
     5161 atttaaagcc ggtgttaccg ctcgtattat catcgtcgat agatacccat gtcccgccgc
     5221 ttccaacaac cgagagattt tcggcaggag tcgaagtgcc gatgccgacg gcgcccgtgc
     5281 tgaaatagac attcgatccg ctcgtaagcc acggactcga gcccgagccc gttccgatgc
     5341 acgaaccgct tattgcaaaa catccggagg tcaaattgat gccggccata gtgcttgttg
     5401 aggtcgtatg aaggcctccc gccacatcaa gcttgtacgc gggcgaggtc gtgccgatgc
     5461 cgacgtctcc ggcggccgtt accacaagct tctggttcga cgactgataa tcggtgccaa
     5521 taccgaagcc agtctgattg ttgccgtctc cgtagtaagt cccggcgaac cagtcgttgg
     5581 agcctgcggt ggtgaactgg atgcccgaca tcctgaactc gttgccgcga tcgagatcga
     5641 gaagcgcgct cgagctgttg ttcgatatgg taacaagcgg gctctgaaaa taatcttcgg
     5701 aatcggccgc cacgtgcaat tttccgaccg gatcggtcgt cgtgccgata tttattgatc
     5761 ccatgaatga ggagccgacg atattattcg gatcgccgac ctccgcgacg gccggattgt
     5821 catagcgagg attggtgtcg gaaacatccg agctcgcgac gtcatacagg ccgtagtcgc
     5881 cgccagtgac gcgagtattg ttgaacgaca tatgatggcc gcctgcaaca tagatgccag
     5941 cttgcccgga gcccgtgccg acccccgata cggtgaagga attcgacgtg aagccccacc
     6001 cgtccagata catgccgtgc cttccgaccg tttccgctcc ggcgctctcg aacgacacgg
     6061 cggtcgcatc gaaggtgtcc gacgtaatgt gatagccgtc tcgtccagca tcatcgcatg
     6121 ccgttgaaat gaacgtgctg taattcaacc cgtcggcaga atagcaatcc tggcccggag
     6181 catccgcgta tgtggagtgg agcgttacgg atgtcgactg tccgccaatc gtcatgccgt
     6241 tccggctcgc gacatccatg cgcgagaagg atatgtccga ttgcatgagc ccggacaagt
     6301 tcatgccgtc gataaatcca taggttctca cgccctcgat atcgacgatc gcagcatcca
     6361 gcgcgccgtc gatatggact ccgtagcctg cggtgcgggt gccgccatac tcgtcgacga
     6421 taaagagatc cttaatcacg atatttctcg tgccggacac ggaagacgat gcgtagagtc
     6481 cgtctgtgtt gctctcgacg taaatcgtcg ccggcccgta aactgtctgg cccgaacgca
     6541 aaaccagggc cgaagtggtt cggtaatcga ctgcgggcgg cgggaagtag acattcaggc
     6601 tcgcgttgag cgccgcctgg atcgccgccg tgtcgtcggt cacgccgtct cccttcgcgc
     6661 cgaagtcctt gacgctcacc ctgtccttca attttgaatc aagcgttctc gaaaccgaac
     6721 tcgtgccggc ctggaggaaa ttgagattga ggccggaaat gctcgacgtg gcggagagcg
     6781 tggtgccgtt cgaagcatat acggcgatct gtcccgtagt gccgctgttc accgtaccgc
     6841 ttcccccgcc gccggaagtg atgcacgtgc cgtttacggc gaaacagcca tttgtcaggt
     6901 tgatgcccgc catggtggaa gacgccgtcg catagaggcc gccaactacc gacagcttcg
     6961 aggaaggcga cgtcgtgccg atgccgaccg aaccgttcgc cttgacggta aagacgtcgc
     7021 cccagccttg agactggttg ctcagcctga aatcgtccgt gccgcttggc atatagacga
     7081 accagtcggc cgcacctgag tttttcaatt gcaggccgac gttgccgctt acgcctccgt
     7141 tatcaacgga aatccaggtg ccgccgctgc cgctgactga gagattttcc tgcggcgagg
     7201 tagtgccgat accgacattg ccggtagtaa aatagatatt cgatccgccc gagagccagg
     7261 ggtttgaacc ggatccgatg cacgtgccgg aaaccgcgaa gcagccggag gtgagattga
     7321 cgccgccgcc aaaggtcgaa gtggcggcgg aagttgaaat gatattcgta gccgtgaggg
     7381 cgccggcaag aagcgcgttg ccattcaccg aaagcgcgga cgacggcgac gtcgttccga
     7441 tgccgacatt gccgccggcg gtcgcaaggt aagtagcgcc ggtcgcagtg aaaagagtcg
     7501 tacttgcgcc gccattgaat cgctgaagtt ggctccatga gttggcggtt gtggttccaa
     7561 acgcgagcga aacgatattg gtcgagcgcg cgagcggata cgagaacgcg agcggatttt
     7621 cgtaatcggt gcccgcgacc gcggctgata cgacacccga accgttcgcc ttaaggatgc
     7681 cgtttacagt gcccgcgccg ccgtagctcg agccgatcac cgtgccctgc cagactccat
     7741 tcgcaatcgt gccgacagcg gcaagattgg caagcgtcgt gatattgggc agggttgaag
     7801 tcgcagagag gcgattatcg aataatg
//
LOCUS       DASZYS010000052         7316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_1152993, whole genome shotgun sequence.
ACCESSION   DASZYS010000052 DASZYS010000000
VERSION     DASZYS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152993"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1788)
                     /locus_tag="VHF05_03105"
     CDS             complement(109..1788)
                     /locus_tag="VHF05_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HVS79944.1"
                     /translation="MVQFNEEKQNERLDELRKREEEELTQRLAIKYGLPYLDLAAVSI
                     NIDALRVLPEDRCRAANLAVFNIVDRELSVGILSPNQPQTQAEIEDLKSRNYQVRLFM
                     VTHQGLNKVWERYKDLSFSFETKSGALDISNDQILELMEKVRTIDDVRNLIKEVIGQN
                     KSYRISRTLEIILSGALALKASDVHLEPEESYVRLRYRLDGVLNDILQFDSETYNLIL
                     SRIKLLSNLKLNIKNRAQDGRFSIKLKDNDIEIRTSLLPGAYNETIVMRMLNPKSIAV
                     PLEELGINSRLLSILEREMEKPNGMILTTGPTGSGKTTTLYACLRKIHTPAIKIITIE
                     NPIEYHLPGIVQTQTDSDENYTFSQGLRSALRQDPDVIMVGEIRDAETAEIAVHSALT
                     GHLVFSTLHTNNAAGTYPRLIDLGINPKILPSAITLSMAQRLVRKLCPICKKQVPLEG
                     KPKQTVDFILSTVTDKSYLDGINASVMYEPVGCNECNGLGYKGRLGIYEAIVTDEILE
                     RIVNENPSEREIKKATAAQDILDMKQDGILKVLQGMTSLDELRRVIDLEAL"
     gene            2218..3426
                     /locus_tag="VHF05_03110"
     CDS             2218..3426
                     /locus_tag="VHF05_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79945.1"
                     /translation="MRTLTVVAAFVAFVSTSYAQDSARVRLADVIQSDAVRESLNSKP
                     DSDNETPAPPDVSPDSPKASTCNPAPYIAPRVREGQKHSGISDAFRDRRYFKLVISNF
                     DPQQDPIVRISDNNFSSKEGPVIIENLCYEGIAPVIYTWYPAMHKDAPWVTLEALTAG
                     GKRYKKTFFLAYTDNATWDIGDPEPRDENYARQQHMPSNYCGQQCAQVVAQPVGRIMN
                     KDQVYCVTYPNAIGCRPPRIGVPSCWGPECAYYMSAMAGITPAVPVYAAGYPYYGYGG
                     YYAGSPYYGGYGYGNFGFNLRLGGFHVGYNSGPPYYYGGGYYGGGYYGYGSYRSYRTY
                     VPSFRNYGGRYYGGSRTSGGSIAGGGSIPSGTIFPGAALPNGGRNIPPGYYGPTPGTY
                     HPSSLTPGLN"
     gene            3514..3948
                     /gene="tsaE"
                     /locus_tag="VHF05_03115"
     CDS             3514..3948
                     /gene="tsaE"
                     /locus_tag="VHF05_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVS79946.1"
                     /translation="MTSESLVETQLLARQFVDGLAPGHSARIVALYGDLGSGKTSFAQ
                     GVASCLKVGEQVQSPTYVIEKIYKTKHSAFKKLVHIDAYRLESSKELEKLGWRDLIAD
                     PDNLILVEWPERVADLMPDNADIVRFEFVDENARKIDFEYGK"
     gene            4081..6552
                     /locus_tag="VHF05_03120"
     CDS             4081..6552
                     /locus_tag="VHF05_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVS79947.1"
                     /translation="MAIKKTAKKTSKKEGKKVQKRLILLDTHAILHRAFHALPDFFSS
                     KGEPTGALYGLVTMLLKIIADLKPDYIVAAYDLPGPTYRHEAYEDYKGTRVKTDDGLV
                     AQIERSNDIYKAFGIPVYSHPGFEADDMLGTIVEELHSNGSIDVVIASGDMDTLQLVD
                     DKKVRVYTLKKGIKDTILYDEEGVRARFGFGPELIPDLKGIMGDASDNIPGVVGVGEG
                     SALKLVAAYGPIENIYKAIEKKGVEAVAKEIGVQKRYVQLVADNREDADFSKMLATIR
                     RDAPIDFKVPQKEWKDAVDMDAVKKLFSELEFRTLGSRVEAAVRGDAKAAKSAESEPA
                     APAPQTATLGEGLFADAGEVINRSELEETSIALWLINSNISTPTLEDIYQFAGTKNFN
                     EARKIVLDELKRRELTFVFEEIEKPLIPIVHKMQDRGVKIDTKCLARLSKKYHAELDK
                     LERGIWEMAGGAFNINSPKQLGQVLFDNLNLVVKGQKKTATGARSTKESELEKLKGMH
                     PIIEKILEYREYQKLLSTYIDNIPKELNSDGRLHAEFHQAGAATGRMSSSNPNLQNIP
                     VKSELGRNIREAFVAAEGHVLLAFDYSQIELRIAAFLSGDEKLREIFRSGQDVHAAVA
                     AQVFNKPVEEITKAERGRAKVINFGILYGMGVNALRQNLGTSRAEAQEFYNQYFETFT
                     TLADYLNKTKAEALRLGFTTTHFGRRRYFEGIRSSLPYIRAQAERMAINAPIQGTQAD
                     IIKLAMRRIDDYLEEEKLDLHAHLLLQVHDELIYEVKEGVADDIAPRIKKIMESIILP
                     KETGGVVFQANASKGQNWERLESIK"
     gene            6552..7268
                     /locus_tag="VHF05_03125"
     CDS             6552..7268
                     /locus_tag="VHF05_03125"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79948.1"
                     /translation="MIYFFYGSDTDTARAKAHALMDGLLAKKPDASLFKFDADNFNTA
                     EFDALSGSMGLFESNNIIFLDRVMENADAEEALLQRLGEMQESPNVIILLEGEPKKVI
                     CDKVGKKAAKAQEFSMREKVAKPEFNSFALADALGRRDKKELWSLFEEAQFAGKVAEE
                     LHGLLFWQMKSMLQAAVSKSAIDAGQKPFVYGKASRFAKNYSLEELKRKSSELVSIYH
                     DARRGGTDMDIALEKFILSL"
BASE COUNT         1715 a         1888 c         2110 g         1603 t
ORIGIN      
        1 cggcggcatt ccatggaatg ccgccgattt ttctcgggag attattctgc cgaaggcgct
       61 ctgcaaaaat tcttattttt gcgctgtttt ctcgtgaagc tacttttcct acaatgcctc
      121 caaatcgatc accctccgta gctcatccaa cgacgtcatt ccctgcagca ccttgagaat
      181 gccgtcctgc ttcatgtcga gaatgtcctg cgccgcggtg gccttcttga tctcgcgctc
      241 gctcggattt tcattgacga tgcgctcgag tatctcgtcg gtgacgatcg cctcgtagat
      301 gccgaggcgc cctttgtagc ctaagccgtt gcattcgttg catccgacgg gctcgtacat
      361 gacgctcgca ttgattccat cgaggtatga tttgtcggta acggtcgaaa gaatgaaatc
      421 gacggtctgc ttcggcttgc cttcgagcgg cacctgcttt ttgcagatcg ggcagagctt
      481 gcgcacgagg cgctgggcca ttgaaagggt aatggcggaa ggaaggattt tcggattgat
      541 gccgaggtcg atgaggcggg gataggtgcc agcggcattg ttggtgtgga gggttgagaa
      601 gacgaggtgg ccggtgagcg ccgaatgaac ggcgatctcg gccgtctcag cgtctcggat
      661 ttcgccgacc atgatgacat cggggtcctg gcgcagagcg cttcggaggc cttgggagaa
      721 ggtgtagttt tcgtccgaat ccgtctgcgt ctgcacgatg cccggcaggt ggtactcgat
      781 cggattttcg atggtgatga tcttgatcgc gggcgtgtgg atcttgcgga ggcaggcgta
      841 gagcgtagtg gtctttccgg agccggtcgg gccggtggtc aggatcatgc cgttcggctt
      901 ctccatttcg cgctccagga tcgaaagaag gcgagaattg atgccaagct cttcgagcgg
      961 cacggcgatc gacttcggat tgagcatgcg catgacgatc gtctcgttgt aggcgccggg
     1021 aagaagcgag gtgcggatct cgatatcgtt gtccttgagc ttgatcgaga agcgtccatc
     1081 ctgggcgcga tttttgatat tgagcttcag gtttgaaaga agcttgatgc gggagagaat
     1141 gagattgtac gtctcggaat cgaactggag gatgtcgttc agcacgccgt cgaggcggta
     1201 gcgcaggcgg acgtacgatt cctccggctc taggtgcacg tcggaagcct tgagggcgag
     1261 cgctccggag aggatgattt cgagcgtacg tgatatgcga tagctcttgt tctggccgat
     1321 gacctccttg atgagattac ggacatcgtc gatcgtcctg accttctcca taagctcgag
     1381 gatttggtcg ttcgaaatgt cgagagcccc gctcttcgtt tcgaaggaga acgagagatc
     1441 cttatagcgc tcccatactt tgttgagccc ctgatgggtg accatgaaga gacggacttg
     1501 gtaattgcgg cttttaaggt cttcaatctc ggcttgcgtt tgtggctggt taggcgagag
     1561 aatgccgacg ctcaattccc gatcgacgat gttgaaaacg gcgagattgg cggcgcggca
     1621 gcgatcttcg ggcaggacgc gcagggcgtc gatgtttatg gaaaccgccg caaggtcgag
     1681 ataggggagg ccgtacttga tggccaggcg ttgggtaagc tcctcctcct cgcgcttgcg
     1741 aagctcgtcg agccgctcgt tctgcttctc ctcgttgaat tgaaccatcc tgttgtgaga
     1801 aatggcatcg ctctttctta agagccgcgc aatttcaaaa ttaattcaaa taatagatcc
     1861 ttttaattta agtagacagt ggctttatca aagagcgtaa actgaatgcc attttctaca
     1921 acgggatcat atggatatat gataccgcac aggcgggaaa gagctagact catttcaaaa
     1981 ataatgacga gagaatactc ttaaaatccg tcgcgaggca cgatgaagct gccgaagcgt
     2041 acttgcagta cgacacggaa gctgaagctg agacgtagta ggattttaag ggtttaatcc
     2101 gtcaaattca ttttctattt ttatagatgg ttatttttga aataaggcta accccagccg
     2161 tagaatttat tgacatttag ctatcaaatg tgatataatg taagaaggag acagcaaatg
     2221 agaacattga cagttgtggc agcgtttgtg gctttcgtct cgacttcgta cgcacaggat
     2281 tccgccaggg tgaggcttgc cgacgtaatt cagtcggatg cagtgcggga atcacttaat
     2341 tccaaaccgg attcagataa cgaaaccccg gcgcctcccg atgtttcgcc ggatagtccg
     2401 aaggcatcga cgtgcaatcc ggcgccctat atcgcacctc gtgtgcggga agggcagaaa
     2461 catagcggaa ttagcgatgc attcagggat cggcggtact tcaagctcgt catttcgaac
     2521 ttcgatcctc agcaggatcc gatcgtccgc atttccgaca ataatttctc ctcgaaggaa
     2581 ggtccggtta ttattgaaaa cctttgctat gagggaattg ctccggttat atacacgtgg
     2641 tatccggcca tgcataaaga tgcgccgtgg gtgacgctcg aagcgctcac agcaggcgga
     2701 aaaaggtata aaaaaacctt ttttctcgcc tataccgata atgcaacatg ggatataggc
     2761 gatccggagc ctcgtgatga gaattacgcg cgtcagcagc acatgccttc gaattactgc
     2821 ggacagcaat gcgcgcaagt agtggcgcag cctgtcggcc gcattatgaa caaggaccag
     2881 gtgtattgcg tgacatatcc gaatgcaatc ggatgccgtc cgccaagaat cggcgtgccc
     2941 tcatgctggg ggccggaatg cgcatactat atgagcgcga tggctggcat tacgcctgcc
     3001 gtgccggtgt atgcggccgg gtatccgtat tacggatacg gcgggtatta tgccggctct
     3061 ccgtattacg gaggttacgg ttatggaaac ttcggtttta atctccgcct cggcggattt
     3121 catgttggct acaatagcgg gccgccatac tattatggag gaggctatta cggcggcggg
     3181 tactacggct acggatcata cagaagttac agaacgtatg tcccgtcgtt tcgcaattac
     3241 ggcggccgct actacggcgg ctctcgaacc agcggcggtt caatcgcggg cggcggctcg
     3301 attccgagcg gcaccatctt cccgggagcg gcgctcccga acggcggcag gaacattcct
     3361 cccggctact atgggcccac accgggcacg tatcatccct catcactgac tccgggattg
     3421 aactgatatt gaactgatct ttctcgggta aaattaaggc gggcgtctca gacgcccgct
     3481 ttttctaata tggtaaaata ggctctaact actatgactt ccgagagcct cgtagaaacc
     3541 cagcttctag ctcgtcagtt tgtggacgga ttggctcccg gccactccgc ccggatcgtg
     3601 gcgctctatg gcgacttggg ctcgggcaag acctcattcg cccagggcgt cgcttcctgc
     3661 ctcaaggtcg gcgagcaggt gcagagcccg acgtacgtca tcgagaagat atacaagacg
     3721 aagcattcgg cgttcaagaa gctcgtgcat atcgacgcgt accggcttga gtcgtcaaaa
     3781 gaactcgaga agctcggctg gcgcgacctc atcgccgacc ctgacaatct catactcgtc
     3841 gaatggccgg agagggtcgc cgatttgatg cccgacaatg ccgacatcgt ccgcttcgaa
     3901 ttcgtcgacg agaatgcccg aaagatcgat ttcgaatacg ggaaataaaa tccaaaggtt
     3961 aaaattcaaa atgcaaaagt ataagtcaaa accgattacg gtataagagt aagtcttttt
     4021 gaattaaaat tttgactttt gacttttgaa atttgattta tgcatccggc tcaaaccaat
     4081 atggcaatca agaaaacagc aaaaaaaacg agcaagaaag aaggcaagaa agtacaaaag
     4141 cgcctcattc ttctcgatac gcacgcgatc ctccaccgcg cattccatgc gctccccgat
     4201 ttcttctcct cgaaaggcga gccgaccggc gcgctctacg ggctcgtgac gatgctcttg
     4261 aagattatcg cggacttgaa gccggactac atcgtcgcgg cgtacgatct tccggggccg
     4321 acgtaccggc acgaggcgta cgaagattac aagggaacgc gcgtgaagac cgacgacggc
     4381 ctcgttgcgc agattgagcg ctcgaacgat atctacaagg cgttcggcat ccccgtgtac
     4441 tcccatccgg gtttcgaggc cgacgatatg ctcggcacca tcgtcgagga acttcacagt
     4501 aatggctcga tcgatgtcgt cattgcatcc ggcgacatgg atacgctcca gctcgtcgac
     4561 gacaagaagg tgcgcgtcta caccctgaag aagggaatca aggatacgat tctctatgac
     4621 gaggagggcg tacgggcccg cttcggcttc ggtccggagc tcattccgga cctcaagggc
     4681 atcatgggcg acgcttcgga caacattccg ggagtggtcg gcgtgggaga aggcagcgcc
     4741 ctgaagctcg tcgccgcgta cggtccgatc gaaaatatct acaaggcgat cgaaaagaaa
     4801 ggcgtcgaag ccgtggcgaa ggaaatcggc gtccagaagc gatacgtgca gctcgtggca
     4861 gacaacaggg aagatgccga tttttcgaag atgctcgcga ccatccgccg cgacgcgccg
     4921 atcgatttca aggttccgca gaaggaatgg aaggacgcgg tcgacatgga cgcggtaaag
     4981 aagctctttt cggagcttga gttccgcacg cttgggagcc gcgtcgaagc agcagtccgc
     5041 ggcgacgcga aggcggcaaa atcggctgag agcgagcctg ccgcgcctgc gccccagacc
     5101 gccaccttgg gagaaggttt gttcgcggat gcgggggaag tcatcaaccg ctctgagctt
     5161 gaggagacgt cgatcgccct ctggctcatc aattcgaata taagcacgcc gaccctcgag
     5221 gatatctacc agttcgccgg cacgaagaat tttaatgagg cacgcaaaat cgttctcgac
     5281 gaattgaagc gccgcgagct cactttcgta tttgaggaga tcgagaagcc cttgattccc
     5341 atagtgcaca agatgcagga tcgcggcgtg aagatcgaca cgaagtgcct cgcgcgcctc
     5401 tcgaagaaat accatgcaga gctcgacaag cttgagcgcg gcatctggga gatggcgggc
     5461 ggcgcgttca atatcaattc gccgaaacag ctcggccagg tgctcttcga caatctcaac
     5521 ctcgtggtca agggtcagaa gaagaccgcg acgggtgcac gctcgaccaa ggaatccgag
     5581 ctcgagaagc tcaaggggat gcatccgatt atcgaaaaaa tcctcgaata ccgcgagtat
     5641 cagaagctcc tctcgacata catcgacaat attcctaaag agctcaattc cgacggaagg
     5701 ctccatgccg agtttcatca ggcgggcgcg gcgaccggca ggatgtcgtc gtcgaacccg
     5761 aaccttcaga acattccggt caaatctgag ctcggccgca atattcgcga ggcgttcgtg
     5821 gcggcggaag gtcacgtgct tctcgccttc gattactcgc agatcgaatt gcggatcgcc
     5881 gcgttcctct ccggagacga aaagctccgt gagatcttcc ggagcggcca ggacgtgcat
     5941 gccgccgtcg cggcgcaggt tttcaacaag ccggtggagg agatcaccaa ggcggagcgc
     6001 ggccgcgcca aggtcatcaa tttcggcatc ctctacggca tgggcgtgaa cgcgctccgc
     6061 cagaaccttg gcacgagccg cgccgaagcg caggaattct acaaccagta cttcgaaacg
     6121 ttcacgacgc tcgccgacta tctcaacaaa accaaggccg aagcgctgag gctcggcttc
     6181 accacgacgc acttcggcag gaggagatat ttcgagggaa tccgctcgtc cctgccgtac
     6241 atccgcgccc aagcggagcg gatggcaatc aatgcgccga tccaaggcac gcaggccgat
     6301 atcatcaagc tggcaatgcg ccgcattgac gactacctcg aagaggagaa actcgatctc
     6361 catgcgcacc tcctgttgca ggtgcacgac gaactcatct atgaggtgaa ggagggggtc
     6421 gccgacgata tcgctccgcg catcaagaaa atcatggaga gcatcatcct gccgaaggag
     6481 accggcggcg tggtcttcca ggcgaatgcg tcgaaggggc aaaactggga aaggcttgaa
     6541 tcgataaaat aatgatctat tttttctacg gcagcgatac cgatacggcc cgggcgaaag
     6601 cgcacgctct catggacgga ctcttggcga agaagccgga tgcgtcgctt ttcaagttcg
     6661 atgcggataa tttcaataca gcagaattcg acgcgctctc gggcagtatg ggtctctttg
     6721 agtcgaacaa catcattttt ctcgatcgcg tgatggaaaa tgccgatgcg gaagaagcct
     6781 tgctccagcg ccttggcgag atgcaggaat cgcccaacgt gatcattctt ctcgaaggcg
     6841 agccgaagaa agtgatatgc gacaaggtcg ggaagaaagc ggccaaagcg caggaatttt
     6901 cgatgcggga gaaagtggca aagccggaat tcaacagctt cgcgctcgcc gacgcgctcg
     6961 gacgccgcga caaaaaggag ctctggtcgc ttttcgagga agcgcagttc gcgggaaaag
     7021 tcgcggaaga gcttcatggc ctgcttttct ggcagatgaa atcgatgctt caggcggcag
     7081 tgtcgaaaag cgccatcgat gcgggacaga agccgttcgt ctacggcaag gcgtcgcgct
     7141 tcgccaaaaa ttattcgctc gaggagctga agcggaaatc ctccgaactc gtttcgatct
     7201 accacgacgc gcgcagggga gggacggata tggacatcgc gctggagaaa ttcatcctgt
     7261 cgctgtaata tttctagaca ttcagtgggt ttttgagtat atttttactg cttttg
//
LOCUS       DASZYS010000053         9661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_825256, whole genome shotgun sequence.
ACCESSION   DASZYS010000053 DASZYS010000000
VERSION     DASZYS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825256"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /locus_tag="VHF05_03130"
     CDS             <1..2353
                     /locus_tag="VHF05_03130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVS79949.1"
                     /translation="DEKEINRRTLSLDDLRIIISHIIHLAVTPHSVADDIDHLGFRRV
                     RYVGELLQQKIRVGMSQIKRNVQNRMSTVESDVTLPVQFISPKPLQARIKEFFTTNQL
                     SQFMQQENPLAEIENLRTLSALGPGGLNRSRAGFEVRDVHPSHYGRVCPIHTPEGPNI
                     GLILRLAIYAKINEFGMIETPYAKVKNGKMTGEIVYLNALDEERYKIAHAATPYDKEG
                     KFLIEEVDVRIKGEPGVVHKSEVDFIDVATNQAFSIATTMIPFLENDDSTRALMGSNM
                     QKQGVPCLIPEAPLVATGIERSAVIDTGRVVLAEEDGTVTAVDGSHIKLKTAKKEKEY
                     KLVNFSRTNGFTAFHQRPIVSVGQAVKKGDVLADTTASDNGQMAIGQNILVGFLSWSG
                     NNYEDAIILSERLVKESKFSSIHIDEVIVNVRDTKLGPEVTTCDIPNVGEAKLKNLAE
                     DGIIRVGAEVRAGDILVGKITPKGETQLTPEERLLRSLFGEKARDVKDTSKRIEPGRR
                     GRVIKVQVFSREKGDKLESGIIKRIHIEIAQLRNVSVGDKLAGRHGNKGVISRVLPVE
                     DMPYMEDGTPIDIILTPLGVPSRMNLGQILELHLGLAAHELGYQAIVPPFSGATGDEI
                     KEELKKAGFPESGKVKLIDGRTGTPFDQDVAVGYMYIMKLDHMVEDKIHMRSIGPYSL
                     ITQQPLGGKAQGGGQRFGEMEVWALLGHGAAYTLREVLTIKSDDILGRSAAFDAIVKG
                     ERIEQVNTPASFNVLLNTLRGLALDVELHKNKRSNSEEVRTED"
     gene            2478..6137
                     /gene="rpoC"
                     /locus_tag="VHF05_03135"
     CDS             2478..6137
                     /gene="rpoC"
                     /locus_tag="VHF05_03135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVS79950.1"
                     /translation="MNNTTPNKKAVDMTDFDSIGLKLASPDRIKEWSYGEITKPETIN
                     YRTQRSEKNGLFDEKIFGPDKDYECYCGKYRGIRYKGIVCEKCGVEITRSIVRRERMG
                     HIELASPVSHIWFLKSMPSRISMVLGIPTPDIERVIYFAGYIITKVSEDERSQFLRDL
                     DSEFKAKVKSAADEKTTEKLKELLLNAKRDIESITEGRVLDEIEYHNFSLRYGTMFEA
                     GIGAEAIYDIFKALDLEKLAESLQKELENAGSVDAVRINKRLSTIQAMIKANLRPEWM
                     FLTSIPVIPPALRPMVPLDGGRYATSDVNDLYRRVINRNNRLRKLKEINAPDVILRNE
                     KRILQEAVDALIDNSIRHGSSLSSAINQTQRRPLKSLSDGLKGKRGLFRQNLLGKRVD
                     YSGRSVIVVGPELHLHQCGLPKHMALELFKPFVISELLAREVAFNIRGASRLIEEGIP
                     EVWAILEEVIQGKYVLLNRAPTLHRLGIQAFQPILIEGNAIQVHPLVCTAFNADFDGD
                     QMAVHVPLSIEAQAEAREIMASDKNILKPGNGNPVISEKLLDIVLGCYWMTKIVPGKK
                     GEGNYFPSPNSAITAFDFGEVDFRAKIHVLATDSEKYKEFGGAIFETTVGRLLFNSVL
                     PSDYPYINSEVERKKIGALISDLIVRYGIEAVPAIMDKIKAFGFKYATYSGTTWSIDD
                     IKIPEQKPAIVKAAREKSEAVMEQFNMGLLSEPERIQKNIEIWLAAKNEIEQLIPETL
                     DPNGPVADMVRSGARGSIAQLTQMAGMKGLMQNTAGETIEFPVVSCSKEGFSPLEYFI
                     TTHGSRKGLTDTALNTAKAGYLTRKLFVVAQDTMISQDDCGTNEGIKFTPEGSSGMQV
                     PIDLVVRGRFLAKDVKGKDGSVLFKRNHYITEEDARAIKNAGVEEVWTRSPMTCASPS
                     GICVKCYGSDLGKGRVIELGEAVGTVAAQAIGEPGTQLTMRTFHAGGTATVGGDITSG
                     LPRVEEVFEKRQPKNPAVVSATAGTVSEIRTSGKEKVIVVIPEIEERKGKHETIEYKA
                     SHTRTVFVKAGDKVKKGDFLTDGSANIDEVFKYGGREKAQEYIIHEISKIYELQGAPV
                     SRKHIEVIIKQMFSRRKIKDAGDTVYARGDIAETREIEIENARVKEAGGKEAKSDGIV
                     MGITEVSLTRKSFLSAASFQHTTRILINGAVRGIEDDLKGLMENVIIGRLIPAGTGFP
                     ESPKAAMVEEVTAEK"
     gene            6260..7993
                     /gene="dnaG"
                     /locus_tag="VHF05_03140"
     CDS             6260..7993
                     /gene="dnaG"
                     /locus_tag="VHF05_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVS79951.1"
                     /translation="MSTSVEKIKERLGIVDVVSSYVELKKAGATWKARCPFHNERTPS
                     FIVSPDRGTFYCFGCHAKGDIFSFVEQIEGLDFKGALKLLAERAGVTLEREDPKARGE
                     HERLFSILEHAKQYFAENLAKTPAAMEYLERRGVKKDTLREWALGYALPEWRGLYDHL
                     RSLQFADAEIENAGLAKRREDAGSGERYYDAFRERVMFPIFDASGRVVGFSGRTLSKD
                     PTTPKYINSPETPLFNKSELLYGLHKAKAAIRKQNYAILVEGQMDLIMCHQAGYANAV
                     AASGTALTASHLERLKKLSPRILMCYDSDDAGQNAAQKNAMLALKLGLEVKIAVLPQG
                     SDPADLLLSDPEGWKKAVANSRHIVDFQLDRLLKSGADSRMKAVALRTSVLPFIAELQ
                     SKIEQSHYVAEAAKRMGIREDAIWEDLRALSRKDGEKRPAASIAPRPQVKAGSMPSLG
                     ERIMGLLYWKPEMEDFRKRVIEIGGEERFLAMKERMAEDSERLIFETEEYYENLSRNS
                     EAPDAIRKDADELLASYERKSLQEDIEKKQSDLRAAEAGSADAEAILKDIHALATRLE
                     DAKRAAGQGNP"
     gene            8076..9350
                     /locus_tag="VHF05_03145"
     CDS             8076..9350
                     /locus_tag="VHF05_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020280323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVS79952.1"
                     /translation="MAVKKQNKKEKKSKSSAQRKQAKKRASATSRTKKREKKDIEKKK
                     KAKAAPKKIAPAKEHRAKSAAKKSGKNDSEEKVERLLTKGRERGFVTYDEILKEFPTV
                     EDDIVFLDELYEKLNTAGVDILESGNLLDDTVEKELQKKYTYNKTDSGFDSVQMYLRE
                     IGQHSLISGADEKDLAKRIQAGDQEAKNLLARANLRLVVSIAKKYVGRSPDLTLLDLI
                     QEGNIGLFKAVDKFDWSKGYKFSTYATWWIRQAITRALADQSRTIRVPVHMVETIAKY
                     KQVVRRLSQDLGRDPLPEEIATEMNMDVEKIYNIEKIDQSTVSLESPVGEDEDKSKIG
                     DFIADEKILSPDQETSRRIVTDQVREILNDLPPKERRILELRHGLIDGVPRTLEEVGK
                     EFGVTRERIRQIEAKAHDKIRQHEKINRLRGY"
     gene            complement(9462..>9661)
                     /locus_tag="VHF05_03150"
     CDS             complement(9462..>9661)
                     /locus_tag="VHF05_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79953.1"
                     /translation="PITRSAKLFPLPEPGEAEAAQDAPLSSIKVFTMLHGNGNLAIYR
                     KVLSCILEMTRIRYKIAIAIL"
BASE COUNT         2356 a         2869 c         2718 g         1718 t
ORIGIN      
        1 cgacgagaag gagatcaatc gtcgcacgct ttcccttgac gatctccgca tcatcatctc
       61 ccacatcatc cacctcgcgg ttacccctca ctcggtggcc gacgatatcg accacctcgg
      121 tttccgccgc gtccgctatg tcggcgaact cctccagcaa aagatccgcg tcggcatgtc
      181 gcagatcaag aggaacgtcc agaacaggat gtccacggtt gaatcggacg tgacgcttcc
      241 cgtgcagttc atctctccga agccgcttca ggcccgcatc aaggaattct tcactacgaa
      301 ccagctctca cagttcatgc agcaggagaa tccgcttgcc gagatcgaaa acctccgcac
      361 gctttccgcg ctcggccccg gaggcttgaa ccgctcgcgc gcaggcttcg aagtccgcga
      421 cgtccatccg tcgcactacg gcagggtctg cccgatccac actcccgaag gcccgaacat
      481 cggcctcatt ctccgcctcg ccatctacgc caagatcaac gagttcggca tgatcgagac
      541 gccgtacgcc aaggtcaaga acggcaagat gaccggcgag atcgtatatt tgaacgccct
      601 cgacgaggag cgctacaaga tcgcgcacgc cgccactccg tatgacaagg aaggcaagtt
      661 cctcatcgag gaagtggacg tccgcatcaa gggcgagccg ggcgtcgtgc acaagtccga
      721 agtcgacttc atcgacgtcg cgaccaacca ggcattctcg atcgccacta ccatgattcc
      781 gttcctcgag aacgacgatt cgacccgcgc gctcatgggt tcgaacatgc agaagcaggg
      841 cgttccgtgc ctcattcccg aggccccgct cgtcgcgacc ggcatcgagc ggagcgcggt
      901 catcgacacc ggccgcgtcg tcctcgccga agaggatggc acggtgaccg cagtcgacgg
      961 cagccatatc aaattgaaga ccgccaagaa ggagaaagaa tacaagctcg ttaacttctc
     1021 gaggacgaac ggctttacag cattccacca gcgcccgatc gtctccgtcg gccaggctgt
     1081 gaagaaaggc gacgtgctcg ccgacaccac cgcgtccgac aacggccaga tggccatcgg
     1141 ccagaacatc cttgtgggct tcctctcatg gtccggaaac aactacgaag acgcgatcat
     1201 cctttccgaa cgcctcgtga aggagagcaa gttctcgtcc attcacattg acgaggtgat
     1261 cgtgaacgtc cgcgatacca agctcggccc cgaagtgacc acctgcgaca ttccgaatgt
     1321 cggagaagcg aaattgaaga atctcgccga ggacggcatc atccgcgtcg gcgcggaagt
     1381 ccgcgcgggc gacattctcg taggcaagat caccccgaag ggcgaaaccc agctcactcc
     1441 ggaagagcgc ctgctccgca gtctcttcgg cgagaaggcg cgcgacgtga aggacacgtc
     1501 gaagcgcatc gagccgggcc gccgcggccg cgtcatcaaa gtccaggtct tctcccgcga
     1561 gaagggcgac aagctcgaga gcggcatcat caagcgcatc cacatcgaga tcgcgcagtt
     1621 gaggaatgtc agcgtgggcg ataagctcgc aggccgccat ggcaacaagg gagtcatctc
     1681 ccgcgtgctt ccggtggaag acatgccgta catggaagac ggcaccccca tcgacatcat
     1741 cctgaccccg ctcggagtcc cgagccgcat gaacctcgga cagattctcg agctccatct
     1801 cggcctcgcc gcgcacgagc tcggctacca ggcgatcgtc cctccgttct ccggcgccac
     1861 cggcgatgag atcaaggagg aactcaagaa ggccggattc ccggaaagcg gcaaggtgaa
     1921 gcttatcgat ggccgcaccg gcacgccgtt cgatcaggac gtcgctgtcg gctatatgta
     1981 catcatgaag ctcgaccaca tggtggaaga caagatccac atgcgctcga tcggacctta
     2041 ctcgctcatc acccagcagc ccttgggcgg caaggctcag ggaggaggac agcgctttgg
     2101 agaaatggaa gtctgggcgc tcctcggcca cggcgccgca tacacccttc gcgaggtact
     2161 taccatcaag tccgacgaca ttctcggaag gtcagccgcg ttcgacgcga tcgtcaaggg
     2221 cgagcgcatc gagcaggtga atactccggc atcattcaac gtattgctca acaccctccg
     2281 cggattggcg ctcgacgtcg agctccataa aaataaaagg tcgaacagcg aagaagtaag
     2341 aacagaggat taattttcaa tttgcaattc ccaattttca atcaatagcc aatgactcaa
     2401 tttccaaatg tttgcacatt ggaatttgaa aattcattga aaattgcaaa attaaaaatt
     2461 gaaaattcca taaaaatatg aacaacacta cacctaacaa aaaggcggtc gacatgacgg
     2521 atttcgattc gatcgggttg aagctcgcat cgcccgacag gatcaaggag tggtcctacg
     2581 gcgaaatcac gaagccggaa accatcaact accgcaccca gcgttccgag aagaacggcc
     2641 tcttcgacga gaagatcttc ggccccgaca aggactatga atgctattgc ggcaagtacc
     2701 gcggcatccg ctacaagggc atcgtctgcg agaagtgcgg cgtcgagatc acccgctcga
     2761 tcgtccgccg cgagaggatg ggccacatcg agctcgcctc gccggtttcg cacatctggt
     2821 tcctgaagag tatgccctcg cgcatctcga tggtgctcgg catcccgacg cccgatatcg
     2881 agcgcgtcat ctacttcgcc ggatacatca tcaccaaggt ttccgaggat gagaggagtc
     2941 agttcctccg cgacctcgat tccgaattca aggcgaaagt gaagtccgct gccgacgaga
     3001 agactaccga gaagctcaaa gagctccttc tcaacgccaa gcgcgatatt gaaagcatta
     3061 ccgaaggccg tgtgctcgac gagatcgaat atcataactt ctcgctccgc tacggaacga
     3121 tgttcgaagc gggcatcggc gcggaagcga tctacgatat tttcaaagcg ctcgatctcg
     3181 agaagctcgc cgagagcctc cagaaggaac tcgaaaacgc cggctccgtc gacgcagtcc
     3241 gcatcaacaa gaggctcagc accatccagg ctatgatcaa ggcgaacctc cgccccgagt
     3301 ggatgttcct gacgagcatt ccggtcattc cgccggcgct ccgcccgatg gttccgctcg
     3361 atggcggccg ctacgcgact tccgacgtga acgacctcta ccgccgcgtc atcaaccgca
     3421 acaaccgcct gaggaagctc aaggagatca acgctcccga cgtcatcctt cgcaacgaaa
     3481 agcgcatcct tcaggaagcg gtcgacgcgc tcatcgacaa ttcgatccgc cacggctcgt
     3541 cgctttcgtc cgcaatcaac cagactcagc gccgcccgct caagtccctt tctgacggtc
     3601 ttaagggcaa gcgcggcctc ttccgccaga accttctcgg aaagcgcgtc gactactccg
     3661 gccgctccgt gatcgtcgtc ggccccgaac ttcacttgca tcagtgcggc cttccgaagc
     3721 acatggcgct cgagctcttt aagccgttcg tcatttcgga gcttctcgcc cgcgaagtcg
     3781 cgttcaacat ccgcggcgcg tcccgcctta tcgaagaagg cattccggag gtgtgggcga
     3841 ttctcgaaga ggtgatccag ggcaagtacg tcctgcttaa ccgcgcaccg acccttcacc
     3901 gcctcggcat ccaggcattc cagccgatct tgatcgaagg caatgccatc caggtgcacc
     3961 cgctcgtatg tacggcgttc aacgcagact tcgacggcga ccagatggca gtccatgtcc
     4021 cgctctcgat cgaagcgcaa gctgaggccc gcgagatcat ggcgtccgac aagaacatcc
     4081 tgaagcctgg caacggcaac ccggtcattt ccgaaaagct tctcgacatc gtgctcggct
     4141 gctactggat gaccaagatc gttccgggaa agaaaggcga gggcaactat ttcccgagcc
     4201 cgaacagcgc gatcaccgcg ttcgacttcg gcgaagtcga tttccgcgcc aagatccacg
     4261 tgctcgcgac ggacagcgag aagtacaaag aattcggcgg agcgatcttc gagacgaccg
     4321 tcggccgcct gctcttcaac agcgttcttc cgtccgatta tccgtatatc aacagcgaag
     4381 tcgaacgcaa gaagatcggc gcgctcatca gcgacctcat cgtccgctac ggcatcgaag
     4441 cggtgcccgc gatcatggac aaaatcaagg cgttcggatt caagtacgcg acgtactccg
     4501 gcaccacgtg gtcgatcgac gacatcaaga ttcccgagca gaagcccgcg attgtgaagg
     4561 cggcccgcga gaagtcggag gccgtcatgg agcagttcaa catgggcctc ctttcggagc
     4621 ccgagcgcat ccagaagaac atcgagatct ggctcgccgc caagaacgag atcgaacagc
     4681 tcattcccga gacgctcgat ccgaacggcc ccgtcgcgga catggtccgc tccggcgccc
     4741 gaggctcgat cgcccagctt acgcagatgg ccggcatgaa gggcctcatg cagaacacgg
     4801 cgggcgagac catcgaattc ccggtcgtgt cgtgctcgaa ggaaggattc tctccgctcg
     4861 agtacttcat caccacccac ggttcgcgca agggcctcac cgacaccgcg ctcaacaccg
     4921 cgaaggcggg ctatcttacc cgcaagctct tcgtcgtcgc gcaggacacg atgatttcgc
     4981 aggatgattg cggaacgaac gagggcatca agttcacccc tgaaggctcg tcaggcatgc
     5041 aggttcccat cgacctggtc gtccgcggac gcttcctggc gaaagacgtg aagggcaagg
     5101 acggaagcgt cttgttcaag cgcaaccact acatcaccga agaggacgct cgcgccatca
     5161 agaacgcggg agtcgaggag gtctggacga ggtcgccgat gacgtgcgca agcccgagcg
     5221 gcatctgcgt gaagtgctac ggctcggatc tcggcaaggg ccgcgttatc gagctcggcg
     5281 aagcggtcgg caccgtggcc gcgcaggcga tcggcgagcc gggcacccag ctcaccatgc
     5341 gcaccttcca cgccggaggc accgcgaccg tcggaggcga tatcacgtcg ggcctcccgc
     5401 gcgtcgagga agtcttcgag aagcgccagc ccaagaaccc ggccgtcgtg tccgcgaccg
     5461 ccggcaccgt ttccgaaatc cgcacgtcgg gcaaggagaa ggtgatcgtc gtcattccgg
     5521 aaatcgagga aaggaagggc aagcacgaga ccatcgaata caaggcgagc cacacgagga
     5581 ccgtcttcgt caaagcaggc gacaaggtga agaagggaga cttcctcacc gacggctcgg
     5641 cgaacatcga cgaggtcttc aagtacggcg gccgcgagaa ggcgcaggag tacatcattc
     5701 acgagatctc gaagatctac gaactccagg gcgcgccagt ttcccgcaag cacatcgagg
     5761 tgatcatcaa gcagatgttc tcgaggcgca agatcaagga tgccggagat accgtctatg
     5821 cgcgcggcga catcgccgag acgcgcgaga tcgagatcga aaacgctcgc gtcaaggaag
     5881 ccggcggcaa ggaagccaag tcggacggca tcgtgatggg catcacggaa gtatcgctca
     5941 ctcgcaagag cttcctttct gcggcctcgt tccagcatac gacacgcatc ctcatcaacg
     6001 gcgcggttcg cggcatcgag gacgatctca aaggcctcat ggaaaacgtc attatcggcc
     6061 ggctcattcc ggcaggaacc ggattcccgg aaagccccaa ggcggcgatg gtggaggaag
     6121 taacggcgga aaagtaattc aaaagtcaaa aatcaaaatg caaagttgtg gtatccgccg
     6181 caagcggatg aagatcattc agccattttg cggctgtccg aaattttgat ctttgaattt
     6241 tacattttga attatcgcca tgtccacaag cgttgaaaaa ataaaagaac gattgggcat
     6301 cgtcgatgtc gtgtcttcct acgtggagct caagaaggcg ggagcgacgt ggaaagcgag
     6361 gtgcccgttc cataacgagc ggacgccgtc cttcatcgtc tctcccgacc gcggcacgtt
     6421 ctattgcttc ggctgccatg cgaaaggcga cattttttcg ttcgtggagc agatagaggg
     6481 cctcgatttc aagggagcgc tgaagctcct ggcggagcgg gccggcgtga ccctcgaacg
     6541 cgaagacccg aaagcgcgag gcgagcatga gaggctcttt tcgatccttg agcacgcgaa
     6601 gcagtacttc gccgaaaatc ttgcgaagac ccccgcggca atggaatatt tggagaggcg
     6661 cggtgtaaag aaggatacgc ttcgggagtg ggccttgggc tatgcgcttc ccgaatggcg
     6721 cggcctctac gaccacctca ggtccctcca gttcgccgat gcggaaatag aaaacgccgg
     6781 cctggcgaag cgccgcgaag atgcgggatc gggagagcgc tattacgatg cgttccgtga
     6841 gcgcgtcatg ttcccgatct tcgacgcttc cggccgcgtg gtgggatttt ccggcaggac
     6901 cctctcgaag gatccgacca cgccaaagta catcaacagt cccgagacgc cgctcttcaa
     6961 caaatcggag cttctctacg ggctccacaa ggcgaaggcc gcgatcagga agcagaacta
     7021 cgcgattctc gtcgaaggcc agatggatct gattatgtgc caccaggcag gctatgcgaa
     7081 cgcggtcgcc gcatccggca ccgccttgac cgcctcccac ctcgagcgct tgaagaagct
     7141 ctcgccgcgc atcctcatgt gctacgactc ggacgatgcg gggcagaacg cggcgcagaa
     7201 gaacgcgatg ctcgccttga agctcggtct cgaggtgaaa atcgcggtac tgccgcaggg
     7261 aagcgatccg gccgacctcc ttctctccga tccggagggt tggaagaagg ccgtcgcgaa
     7321 ctcgcggcac atcgtcgact tccagctcga tcgccttctc aaaagcggcg cagacagcag
     7381 gatgaaggcg gtcgcgcttc gcacgtcggt cctgccgttc atcgcggagc tccagtcgaa
     7441 gatcgagcag tcgcattatg tcgccgaagc agcgaagcgc atgggtatcc gcgaagacgc
     7501 gatctgggag gatctcaggg ctctttcgcg aaaagatggc gaaaaacggc ccgcggcttc
     7561 cattgccccg cgccctcagg tgaaagcggg gagcatgccg tcactcgggg agcgcatcat
     7621 ggggcttttg tactggaagc cggagatgga ggatttcagg aagcgcgtca tcgaaatcgg
     7681 cggcgaggag agatttctcg cgatgaagga gcggatggcg gaggatagcg agcgcctcat
     7741 attcgaaacc gaggaatact acgaaaatct ttcaaggaat tccgaggcgc ccgacgcgat
     7801 tcgcaaggac gcggacgagc ttctcgcgag ctacgagcgc aagagcctcc aggaggatat
     7861 tgagaagaag cagagcgatt tgagagcggc ggaggcgggc agtgcggatg ccgaagccat
     7921 cctgaaagat attcatgcac tcgctacccg tttggaagac gcgaagcggg cggcagggca
     7981 ggggaatcca taagggagca aaattttcga atcacagttt aatttcattt tcaacttccc
     8041 aacaatatcg agcgtgttat aataaaatgt acgcaatggc agtcaaaaag caaaacaaaa
     8101 aagagaaaaa gtcaaaatcc tcggcacaga ggaaacaagc gaaaaaaaga gcctccgcaa
     8161 cctcccgcac taagaaaagg gagaaaaagg atatagagaa aaagaagaag gccaaggcag
     8221 ctccgaagaa gattgcgccg gccaaagaac atcgagccaa gtccgccgcg aaaaaaagcg
     8281 gcaaaaacga ttccgaggaa aaagtggagc gtttgcttac gaagggacgc gagcgcggtt
     8341 tcgtcacgta tgacgaaatc ctcaaggaat ttccgacggt cgaagacgac atcgtctttc
     8401 tcgacgagct ctacgagaag ctcaataccg cgggcgttga tattctcgag agcggcaacc
     8461 tgctcgatga caccgttgag aaggagcttc agaagaaata tacctacaac aagaccgatt
     8521 ccggattcga ttccgtgcag atgtacctcc gcgaaatcgg ccagcattcg ctcatttccg
     8581 gcgccgatga aaaggatttg gcgaagcgca ttcaggcggg cgaccaggag gcgaagaacc
     8641 ttctcgctcg cgccaacctg aggcttgtcg tttcaattgc caaaaaatac gtcggacgct
     8701 cgccggacct cacgcttctc gacctcattc aggaaggcaa catcggcctc ttcaaggctg
     8761 tcgacaagtt cgactggtca aaaggctata aattctcaac gtacgccacg tggtggatcc
     8821 gccaggccat tacccgcgcc cttgccgacc agtcccgcac tatccgcgtg ccggtgcaca
     8881 tggtggaaac catcgccaag tacaagcagg tggtgaggcg cctctcgcaa gacctcggcc
     8941 gcgatccgct tccggaggag atcgccacgg aaatgaacat ggacgtggag aagatctaca
     9001 acatcgagaa aatcgaccag tcgactgttt ccctcgaaag cccggtaggc gaggatgagg
     9061 ataagtcgaa gatcggcgat tttatcgccg acgagaaaat cctctctccc gaccaggaga
     9121 cgtcgcgccg cattgtaacc gatcaggtgc gcgaaattct caatgactta ccgccgaagg
     9181 agcgcaggat tctcgaactc cgccacgggc ttatcgacgg agtgccgaga accctcgagg
     9241 aagtgggcaa ggaattcggc gtgacccgcg agcgcatcag gcagatcgag gcgaaggctc
     9301 acgataagat acgccagcac gaaaaaatca atcgcttgag aggttactag gatcgaaatc
     9361 taaaactgat aacgtcaaga ctcgaaaaca ggccctcggc gcctgttttc ttttgaattg
     9421 ccgttttccg cgaccgcgga aaacggcaat gtttccatac gttataaaat tgctatagca
     9481 attttataac gtatgcgagt catttctagt atgcatgaca aaacctttcg atatatcgca
     9541 aggtttccat taccatgcag catagtgaac acttttatag aagaaagcgg cgcatcctgc
     9601 gccgcttcag cctcgcctgg ttcagggaga gggaacaatt ttgccgagcg tgtaatgggg
     9661 c
//
LOCUS       DASZYS010000054        10403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_432212, whole genome shotgun sequence.
ACCESSION   DASZYS010000054 DASZYS010000000
VERSION     DASZYS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432212"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..675)
                     /locus_tag="VHF05_03155"
     CDS             complement(82..675)
                     /locus_tag="VHF05_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79954.1"
                     /translation="MAAVVNGGSYQAAQTTLPALNTTHTEIQAVVDPRPAGDTLEEVT
                     LWNGEDLTPSTTLNLLPDANKATTSQKASAKRAVTVKLASATYISSAAVEKAVEQYFA
                     DIPVMIDIARCESQFRQYDAAGDVLHGKVNYADRGVMQINEAYHSAEALKLGMDILTL
                     QGNMEFARHLYDEQGTQPWISSSSCWGGSDSTQIAQK"
     gene            complement(1017..1589)
                     /locus_tag="VHF05_03160"
     CDS             complement(1017..1589)
                     /locus_tag="VHF05_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79955.1"
                     /translation="MLQEPTVGSTQPHFRIRPLDDFPPRLTIEGIPHTTENTAFVKTG
                     EGFEPLPSRTIIIKTKEKSPVTGELPTVRNPKPRLVVFIVGGMYMPESSLDLISMREG
                     DSRMPLPLMVEPICGKTAQSLCTSEPVTEAETFLRTRYSLEYYSVESIQRLKPGDYKL
                     VYAPPVNPQPEKVQYWLLRISPGDNPLAPK"
     gene            complement(1867..2760)
                     /locus_tag="VHF05_03165"
     CDS             complement(1867..2760)
                     /locus_tag="VHF05_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79956.1"
                     /translation="MNPAQRIEGNFPFAFSRILPALGRHAKILLMALAIFLFFCGTIF
                     AGTTLTNETIAKMANDGVPVDAIVATINSAPQANFDTSFSALGSLATNQHVPDAVLKA
                     MQAKMQAHTAAPEVSTPSVQSAESDHNGLNVTLIDAKGRQYQLNDETQTPEMRGGGGK
                     MLAQYLIPGGIFIFKNKLDMTLPGDHSDIKVSNPTKITIAGLPSRARSHAGKAVKLVE
                     LEVHDGTRIVQTSSSLLPKIHAFRSKSAFDLKSSVEGKDVILTFDPLNPGHYALVGGP
                     AAYAFDVVASPSTEPTQISSR"
     gene            complement(2904..3497)
                     /locus_tag="VHF05_03170"
     CDS             complement(2904..3497)
                     /locus_tag="VHF05_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HVS79957.1"
                     /translation="MIVVDVETTGTEAHKHSLLSIGAVDFDDPARRFYGECRMRDGAH
                     IMDGALEINGFTEEEIRDPKKMAPSELAAKFVVWALEARDHTVGGQNPYLDYDFIRES
                     AEIGHEDFPIAKRTLDLHTVCWMHIVMNGGEPPIANKRSNINSAFICHYVGIPEEGKP
                     HIGLNGALWEAEAFSRLLYGKNFLPEFAGFPLPEWKG"
     gene            complement(3596..4006)
                     /locus_tag="VHF05_03175"
     CDS             complement(3596..4006)
                     /locus_tag="VHF05_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HVS79958.1"
                     /translation="METPNEQEHSKHSALEHWLIAGLIILVAALAFGLGRLSTLEEAK
                     VPVTIDYFSSSTPAAAASAPIPKSAPASDTSAGTAAKSGNANATGSIVASKSGTTYYY
                     ASCSNRISDANKIYFDTAAAAEAAGFRLAKTCTP"
     gene            4191..4463
                     /gene="rpmA"
                     /locus_tag="VHF05_03180"
     CDS             4191..4463
                     /gene="rpmA"
                     /locus_tag="VHF05_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HVS79959.1"
                     /translation="MAHKKSAGTTRNGRDSQPKYLGIKINHGEKAAVGEVIVRQRGTD
                     TLPGANVGMGRDHTLFALKEGVVEYSTKRKIHFDNSVKRKKIVNIK"
     gene            complement(4812..5255)
                     /gene="rplI"
                     /locus_tag="VHF05_03185"
     CDS             complement(4812..5255)
                     /gene="rplI"
                     /locus_tag="VHF05_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVS79960.1"
                     /translation="MKVILLKDIRNVGKKYEIKEVAEGFAMNSLIPRNLATAATAGAM
                     KRLDGMKAQDMAARSARDAETAKNLEKIEGVEITMSGKANEGGHLFAAIHKDDIIDEL
                     RKQSGLDIEADHIVLDKPVKEAGKHKIPVAVGGKTAEFTLVVETA"
     gene            complement(5400..6623)
                     /locus_tag="VHF05_03190"
     CDS             complement(5400..6623)
                     /locus_tag="VHF05_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVS79961.1"
                     /translation="MRKIFNKGLVSVLVAVLCLGGTFYAGYATGAHNSARLITGVYNT
                     SNGAQAGVDFSPFWKAWSELNDKFVPTSTTSEAVTDQDKVWGAIAGLTASLGDPYTVF
                     FPPAESSVFEGDISGNFDGVGMEIGTRNDILTVISPLKDTPAYKAGIKSGDQILAIGA
                     TSTAGMSSDEAVELIRGAKGTTVTFTILHQGDKDPVKIPVVRDTIDIPTIDTKTLPGG
                     VFDIELYNFSAVSANQFRSALRQFVASGSHKLILDLRGNPGGYLDAAIDMASWFLPSG
                     TTVVREDFGGKQDEQVYKSKGYDIFNNQLKMAILVDGGSASASEILAGALSENGVAKL
                     VGQQTFGKGSVQELVKITPDTSLKVTVARWLTPNGISISHNGLTPDYAVPLTADDVAA
                     GRDPQLDKALEILNQ"
     gene            6748..7662
                     /locus_tag="VHF05_03195"
     CDS             6748..7662
                     /locus_tag="VHF05_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HVS79962.1"
                     /translation="MINKHAFNGSTWIELDHPTIEEVRTVMDEYAIHPKIARELVSPS
                     LRQRLEGYEDHLYLILHFPAFRHTHKEENVQEIDFVIGRNYVITVKYDTVDSLHKFSK
                     VVEVDSILRRSAQYDDPAYIFFGILHEIYRSLLEEIAYLEDWTEDIERNIFKEKEREM
                     VFTLSHVGRNLLDLKKIVEQHKELLDDLEEQGGAAFGEKFMAAARDLKADFFKVRALI
                     RGNLEMVQELRETNNSLVTTKQNDAMKTLTLLAFVTYPLSLLAAVFGMNTTGMPLVGN
                     PYDFWVIVGVMLAAAACMFVYFKRKRWL"
     gene            7856..9250
                     /gene="cysS"
                     /locus_tag="VHF05_03200"
     CDS             7856..9250
                     /gene="cysS"
                     /locus_tag="VHF05_03200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVS79963.1"
                     /translation="MDIRLFNTYSGTKEEFTPLSRGSVGMYHCGPTVYNYAHIGNLRA
                     YVFADTIRRTFEFAGYKVKQVVNITDVGHLTSDADTGKDKIEERARTENKSASEIANF
                     YTEAFFKNLKDLNVNTAGTLFPKASEHIKEQIDLIRRLEEKGFTYKTSDGIYFDTAKF
                     KDYGKLGHVDLAGQVEGARVEINSEKRHPTDFALWKFSQPYEKRLQEWESPWGVGFPG
                     WHIECSAMSMKYLGETFDIHTGGIDHIPVHHNNEIAQSVCATGKEFARYWMHSAFVQV
                     RGEKMAKSKNNFLTLDGLAEEGVSPLAYRYWLLTSHYRTPVNFTDEAAENAGTTLEKL
                     KRFIGKAKMGGAPLPEWIAKFERLIGDDFDTPGAIALMWKLIRNRSEKEEDKRATVLE
                     FDKVLGLGLAELPQKTVSQRVPKEVKDLLKKRESARAEKDFASSDSLRDEIARLGYRV
                     MDTPDGQVVEALPR"
     gene            9310..9678
                     /locus_tag="VHF05_03205"
     CDS             9310..9678
                     /locus_tag="VHF05_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79964.1"
                     /translation="MSLLLAIADVLARTGIRSFHLDKNKVEIVTLDRSYKPAELLSNG
                     HFSADTRPATPEELDIFALKYHVDKNSPAIMTVAQINGAEKSSVSVFRADERGVYRWD
                     GLYTLWPGTHILVVKEARST"
     gene            9896..>10403
                     /locus_tag="VHF05_03210"
     CDS             9896..>10403
                     /locus_tag="VHF05_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HVS79965.1"
                     /translation="MAEYTINTSSIRIPPQSLEAEMALLGSIMLRPEAMYEVLDAVSP
                     AYFYADRHRLIFETMQELFSKSVPIDFLSLSSRLKEKQLLEQVGGNVFITELASLVPS
                     SASVRHYADIVRKKHMMRKLIEASEDIARIGFDESADLEESLDAADKKIFEITNQGGS
                     HKFVEMKDT"
BASE COUNT         2329 a         2689 c         2841 g         2544 t
ORIGIN      
        1 gaaattcgcc gagcatagca aaagcccgtc gactggacgg gctttatgcg tgtttcctca
       61 aaagcaatat tgaacggatt tttacttctg tgcaatctga gtcgaatccg atccgcccca
      121 gcagctcgag gacgagatcc acggctgggt gccctgctca tcgtagagat ggcgggcgaa
      181 ctccatattg ccctgaagag tcagaatgtc catgccgagc ttcaaggctt cggcgctgtg
      241 ataggcttcg ttaatctgca tcacgccgcg gtcggcataa ttgacctttc cgtgaagcac
      301 gtcccctgcc gcgtcgtact gcctgaactg cgattcgcag cgggcgatat cgatcatgac
      361 gggaatatcg gcgaaatact gttccaccgc cttttccact gccgcggaac tgatgtaggt
      421 tgccgaggcc aatttgacgg tgaccgccct cttggccgaa gccttctggc tggtggtcgc
      481 tttgttcgcg tccggcaaga gattcagggt ggtcgaaggg gtaagatcct ctccgttcca
      541 aagcgttact tcttctaaag tgtctcctgc cggcctcggg tcgacgacgg cctgtatttc
      601 cgtatgggtt gtgttaagag cagggagagt ggtctgtgcc gcctggtagc tgccgccatt
      661 tacgactgcg gccaaggcaa gtactgcgcc gagtttagtg aagtttaaga tgcattttcg
      721 ttagactggt aattctgtcg tgtctggaaa tgaaaaaact gagttttcac tcagctgggt
      781 acattatagc atattggagg ccattgtcaa tgaatttttt ttaaattttc ctaaataacg
      841 gctctgtgga gccactattt taagctcttc actagaccat atttttagct tttttgggat
      901 accccatctt tcccctcccc ttttccgctc tttggccgga ttcggacgct tttgagaggc
      961 aaaaaagaag cccgagaagc gcattcatgc gccgctcggg ctttcgtctt cactttttac
     1021 tttggagcga gcggattatc gcctggcgat atccgcagga gccagtactg caccttttct
     1081 ggttgaggat taactggcgg agcgtacacg agcttgtaat cgcccggttt caaccgctgt
     1141 atagattcta ccgagtaata ctcgagcgaa taacgggttc tgaggaaagt ttcagcttcc
     1201 gtcaccggtt cgcttgtgca gaggctttgg gcagttttgc cgcaaatcgg ttcgaccatg
     1261 agcgggagcg gcattctgct atctccttca cgcatcgaga tgagatcaag agaagactcc
     1321 ggcatgtaca ttcctccgac aataaacacc acgaggcgag gtttcggatt cctcacggtc
     1381 gggagctctc cggtgaccgg gcttttttcc ttcgttttga taatgattgt cctcgaaggt
     1441 aatggttcga aaccctctcc agttttaaca aaggcagtat tttcggtcgt atgcgggatt
     1501 ccctcgatag tgaggcgcgg cggaaagtcg tcgagcgggc ggatgcggaa gtgcggctgg
     1561 gtcgatccga cagttggttc ctggagcaat gattgcgagt tgtccgaacg agcaaagacg
     1621 gcaaatggcg caatgcatgc gcatgccaag actgctgtta atagtttcaa tgtagctctc
     1681 cttttgtgac ggtagttcta tattcttaat ataatattat tcaaatattg tcaattcgtt
     1741 gtatgcactg ccaaaacaaa agcccgacgc atgaagcgaa gggcttagtt ttaaattcaa
     1801 aaagaaggta ctgcaaaaat gtgtttttat ccgtctcctt tcttttcagt ctaaaagaag
     1861 atcgggttag cgggagctga tctgggtcgg ctctgtgctt ggagatgcga cgacatcgaa
     1921 tgcgtacgcc gcaggaccgc ctaccagggc ataatgcccc ggattaagag gatcgaacgt
     1981 gagaatcaca tcttttccct ctacggatga tttgaggtcg aaagcgctct tactcctgaa
     2041 agcgtggatt ttgggaagca aacttgaact tgtctggacg atgcgggtac cgtcgtggac
     2101 ttctagttcg acgagtttta ccgctttgcc agcatgcgac ctcgctcgcg acggcaggcc
     2161 agcaatcgtg atttttgtcg gattacttac ctttatgtct gaatggtcgc ccggaagagt
     2221 catatccagc ttattcttaa agatgaatat gccgccgggg atcagatatt gcgctagcat
     2281 tttcccgccg ccgccgcgca tctcgggcgt ttgggtctcg tcattcagtt ggtattgacg
     2341 gccttttgcg tcgattaacg tcacgttcag gccgttgtga tcggattcag cggattgcac
     2401 actgggcgtc gagacttccg gtgccgcagt gtgagcttgc attttggcct gcatagcctt
     2461 gagaaccgcg tccggaacat gctgattggt tgctaacgaa ccaagcgctg aaaacgaggt
     2521 gtcgaaattt gcctgcggcg cactgttgat tgttgcgacg atggcatcga cgggtacgcc
     2581 gtcgttcgcc attttagcga ttgtttcgtt cgtgagcgtt gttcctgcga atatggttcc
     2641 gcagaagaac aggaagattg ccaaggccat gagaagaatt tttgcatgtc ttcctagggc
     2701 gggcaggatg cgggaaaaag caaaggggaa attcccctcg attctttgtg cgggattcat
     2761 ttttgcacct ttctttattg aattttcagg gtactactat cttggtacct tagacggttg
     2821 gccgcctaaa ctggttagat tatgacaata ttcaaatgaa aagtcaatga ggcacagctg
     2881 tgccctgtgg acaaacggct gggctaccct ttccactcgg gaagcgggaa accggcgaat
     2941 tccggaagga aatttttgcc gtagaggagg cgcgaaaaag cctccgcttc ccacagcgcg
     3001 ccattgagac cgatgtgcgg cttcccttct tcgggaatgc cgacatagtg gcagataaat
     3061 gctgaattga tgtttgagcg cttgttggcg atcggcggct cccctccgtt catgacgata
     3121 tgcatccagc agacggtgtg gaggtcgagc gtgcgttttg cgatcggaaa atcctcgtgg
     3181 ccgatttccg ccgactcgcg gatgaaatca tagtcgaggt acgggttctg ccctcctacc
     3241 gtgtggtcgc gcgcctcaag cgcccagacg acaaacttcg ccgcgagctc tgaaggcgcc
     3301 atctttttcg gatcgcgtat ctcttcttct gtgaatccgt ttatctcgag cgccccatcc
     3361 atgatatgcg cgccatcgcg cattctgcat tcgccgtaga agcggcgggc gggatcgtcg
     3421 aagtcgactg ctccgatgga gaggagcgag tgcttgtgag cttcggtacc ggttgtttct
     3481 acgtctacta cgatcatata gtaattttca atttaaaatt tttaattttc aatgaatttt
     3541 caaattctaa tgtttcaagc gtttgaaaat tgcgaattgg aaatttgtat cgtatctacg
     3601 gtgtgcatgt ttttgccaag cgaaaccccg ccgcctccgc cgccgcggcc gtatcgaaat
     3661 aaatcttgtt tgcgtcggag atgcggttgg agcatgaagc gtagtagtac gtggtgccgc
     3721 tcttcgaagc aacaatggag cccgttgcgt ttgcattgcc tgacttcgcc gcggtgccgg
     3781 ctgatgtatc gcttgccgga gctgatttcg gaataggcgc ggaggccgcg gcggcgggag
     3841 tcgaggagga gaaatagtcg atcgttaccg gcactttcgc ttcctcgaga gtcgagagtc
     3901 ttcccaagcc gaaggcgagc gccgcgacga ggataatgag cccggcgatg agccagtgct
     3961 cgagggcgga atgcttggaa tgctcttgtt cgttcggggt ttccataggc ggcgtttaat
     4021 tgaccctgta gtagattttg gcatcgcaat cccttgcggg attgcgggca gggcccgatg
     4081 ccaaaattca ctacagggtt gattttttcc cttatctctt ttatactcct taagtcttat
     4141 tatagcaggt ttgagggcta attccctcac tatttagaga gtttagagcc atggcacata
     4201 aaaagtcagc aggaacaact agaaacggcc gagattcgca gccaaaatac ctcggcatca
     4261 agataaacca cggcgagaaa gctgccgtgg gggaggttat cgtgcgccag agaggcaccg
     4321 atactcttcc tggagcgaat gtcggaatgg ggcgcgacca caccctcttt gcattgaagg
     4381 aaggcgtcgt tgaatactcc accaagcgca agatccattt cgacaactcg gtgaagcgca
     4441 agaagatcgt caacatcaaa taatcaagca aaatgccggc atatgacggc attttgcttg
     4501 ctcaaatgag ggagttttga ttttgaaaga accccgggta ttacccgggg gcggttgagg
     4561 ctggctaaag gccgccgtgt gccgcgaacc aaaaaagggc ggcgatgccg ccggttaaaa
     4621 caagcagggt cggaattgct attcggcatg ccgatctgca tttggcgctg agtgtttttt
     4681 ccgtagcagt gtactgcgaa aacatctgtt tactgtctcc ttgtcagcat ggtgctgttc
     4741 tttctttgaa taaaataaca gccccgctag gggctgtcaa tcaattttcc ggatctcgga
     4801 agagatttcc gttatgcagt ctcaacgaca agcgtgaatt ccgcagtttt gccgccgacc
     4861 gccacgggga tcttatgctt ccccgcttcc ttgacgggct tgtcgagaac gatgtggtcg
     4921 gcttcgatat cgaggccgct ctgcttgcgg agctcgtcga taatgtcgtc cttgtggatc
     4981 gcggcgaaaa gatggccgcc ctcgttcgcc ttgccgctca tggtgatctc aacgccttcg
     5041 attttttcga ggttcttggc tgtttccgcg tcgcgggcgg aacgcgccgc catgtcctga
     5101 gctttcattc catcaaggcg cttcatggcc cctgccgtag cggcggtcgc caaattgcgg
     5161 ggaatgagcg agttcatcgc gaagccttct gcgacttcct ttatttcgta ttttttgccg
     5221 acgttgcgga tgtccttgag aagaattact ttcatgcgtt tttacgttta aataagcagt
     5281 caacagtatg accgagaacg gagtgaaaat caaccctgta gtgagttttg gcttctggct
     5341 ttgcctgtct cgcaaagcga gacaaagcca aaatctacta cggggtcaac ccgtcgcttt
     5401 tactggttca gaatttcgag ggctttgtcg agctgggggt cgcgtccagc ggcgacatcg
     5461 tcggcggtga ggggcactgc ataatcaggc gtgaggccgt tgtgggagat cgagatgccg
     5521 ttcggagtga gccatcgcgc cacagtcact ttaagcgatg tgtccggggt gatcttcacg
     5581 agctcctgga cggagccttt gccgaaagtc tgctggccga caagcttcgc cacgccgttc
     5641 tcgctcaggg ctcctgcgag aatttcggat gcgcttgccg atccgccgtc gaccaggatg
     5701 gccatcttga gctggttgtt gaaaatatca taccctttgc tcttgtagac ctgctcgtcc
     5761 tgcttgccgc cgaaatcctc gcgcacgacc gtcgtgccgc tcggaagaaa ccagcttgcc
     5821 atgtcgatcg ccgcgtcgag atagccgccc gggttgccgc gaagatccag gatgagcttg
     5881 tgcgagcctg aggcgacgaa ctggcgaagc gccgatctga actggttggc ggaaacggca
     5941 gagaaattgt agagttcgat atcgaagacg ccgcccggaa gggtcttggt gtcgatggta
     6001 ggaatgtcaa tcgtgtcgcg caccaccggg atcttcaccg gatctttgtc gccctggtgg
     6061 agaatggtga acgtgaccgt cgtgcccttc gcgccgcgga tgagttcgac ggcttcatcg
     6121 gaggacatgc ctgcggtcga tgtggcgccg atggcgagga tttggtcgcc cgacttgatg
     6181 ccggccttgt atgccggcgt gtccttcagg ggcgaaataa cggtgaggat gtcgtttcgc
     6241 gtgccgatct ccatgccgac gccgtcgaag ttgccgctaa tgtcgccttc gaaaacggac
     6301 gattcggccg gcgggaagaa gacggtgtac gggtcgccga gagaggctgt gaggcccgcg
     6361 atggcgcccc agaccttgtc ctggtcggtt acggcttcgc tcgtggtcga cgtcggaacg
     6421 aacttgtcgt tgagctcgct ccaggccttc cagaagggcg aaaagtcgac gcctgcttgc
     6481 gcgccgttgc ttgtgttgta gacgccggtg ataaggcgcg cgctgttatg agccccggtt
     6541 gcataccctg cgtagaatgt gccgccgagg cacagaacag ccacaagaac cgaaacgaga
     6601 cccttgttga aaattttcct catgaagaaa tagttagttc attcattatc tcagatggtg
     6661 gcgcagaggg tcaaacgtgg ataacgctcg gattccctat agtataatga ttcgtaactt
     6721 ttgcgcggcg gcccgcgcgg tacgagtatg atcaacaagc atgccttcaa cggctcgacc
     6781 tggatcgagc tcgatcatcc cacaatcgag gaagtccgta ctgtcatgga cgaatatgcg
     6841 atccatccca agatcgcgcg cgaactcgtt tcgccctcgc tccgtcagcg cctcgaaggc
     6901 tatgaggatc atctctatct catcctccat ttccccgcct tccggcatac tcacaaggag
     6961 gagaacgtcc aggagatcga cttcgtcatt ggccgcaatt acgtcatcac cgtaaagtac
     7021 gatacggtcg acagccttca caagttttcc aaggtcgtgg aagtcgactc gattcttcgg
     7081 cggagcgcgc agtacgacga tcccgcgtac atcttcttcg gcatcctcca tgagatctac
     7141 cgctcgcttc tcgaggagat cgcctatctc gaagattgga cggaagacat tgagcgcaac
     7201 atcttcaagg aaaaggagcg cgagatggta ttcacgctct cgcacgtcgg gcgcaacctt
     7261 ctcgacctca aaaagatcgt cgaacagcac aaggagctcc tcgacgactt ggaggagcag
     7321 ggcggcgccg cgtttggcga gaagttcatg gccgccgcgc gcgatctcaa ggccgatttc
     7381 ttcaaggtgc gcgcccttat ccgcggcaat ctcgaaatgg tgcaggagct tcgcgagacc
     7441 aacaattccc tcgtgaccac caagcagaat gacgctatga agacgctcac cctgctcgca
     7501 tttgtgacct acccgctctc actcctcgcg gccgtgttcg gcatgaacac gaccggcatg
     7561 ccgctcgtcg gcaacccgta tgatttctgg gtgatagtag gagtgatgct tgccgccgcg
     7621 gcctgcatgt tcgtttattt caagcgcaag cggtggctgt aagtatcgta gacgcaattt
     7681 ataattttca attatcaatt tgcaatgaat tttaaatttt aaatgattca aacgtttgaa
     7741 cctgcctgcc ggcaggcagg aattaattca ttgagatttg attgaaaatt gcaaattaca
     7801 aattgaaaat tacctcatgt tttacatttt gatattttca ttttacattt agcgcatgga
     7861 tattcggctt ttcaatacct attcggggac gaaggaggaa ttcactcccc tttcccgcgg
     7921 ttcggtcggc atgtaccatt gcggcccgac cgtctacaac tatgcccata tcggcaatct
     7981 ccgcgcctac gtctttgccg acacgatccg ccgcacgttc gaattcgccg gctacaaagt
     8041 gaagcaggtc gtgaacatca ccgatgtcgg ccacctcacc tccgatgcgg acaccggcaa
     8101 agacaagatc gaggaaaggg ccaggacgga aaacaaatcc gcctcggaga tcgccaattt
     8161 ctacaccgaa gcgttcttca agaatttgaa agacctcaat gtgaataccg cgggcacgct
     8221 tttcccgaag gcaagcgaac atatcaagga gcagatcgat cttatccgga ggctcgagga
     8281 gaaaggcttc acgtacaaga catccgacgg catctatttc gataccgcga aattcaagga
     8341 ttacggcaaa ttgggccacg tcgaccttgc gggccaggtg gagggcgcgc gcgtcgagat
     8401 caattccgaa aagcgccacc cgaccgactt cgccctttgg aaattctccc agccgtacga
     8461 gaagcgcttg caggaatggg agagcccgtg gggcgtcggc tttccaggct ggcacatcga
     8521 atgctcggca atgtccatga aatatcttgg cgagacgttc gacatccaca ccggcggcat
     8581 cgatcacatt cccgtgcacc acaacaacga gatcgcccag tccgtctgcg ccaccggcaa
     8641 ggaattcgcg cgctattgga tgcacagcgc gttcgtccag gtgcgcggcg aaaaaatggc
     8701 aaagtcgaaa aataatttcc ttaccctcga cggccttgcc gaggaaggcg tctcccctct
     8761 tgcctaccgc tattggcttc ttacctcgca ctaccgcact cctgtcaatt tcaccgacga
     8821 ggctgcggaa aatgccggca cgacgctcga aaagctcaag cgcttcatag gaaaggccaa
     8881 aatgggcggc gcaccgcttc cggaatggat cgccaagttc gaaaggctca ttggcgatga
     8941 tttcgatacg ccgggggcca tagcgctcat gtggaagctt atccggaatc gcagcgaaaa
     9001 ggaggaggac aagcgcgcga ctgtgctcga attcgacaag gtgcttgggc tcggactagc
     9061 cgagctcccg cagaaaaccg tttcccagcg cgttcccaag gaagtgaaag atctcttgaa
     9121 gaagcgcgag agtgcacggg ccgagaagga ttttgcttct tctgacagcc tccgcgacga
     9181 gatcgctcgc ttaggctatc gcgtcatgga cactcctgac ggccaggtgg tagaggctct
     9241 cccccgctag ccactgttct attgcaaaaa taattaaata tggtattata aaagcagagg
     9301 tgaagaacca tgagcttact tttggcaatt gccgacgtgc tcgcgcgcac tggcatccga
     9361 tctttccatc tggataaaaa caaagtcgag attgtgacgc tcgacaggtc gtataagccg
     9421 gcggaactgc tttcaaatgg gcacttcagt gccgatacgc gtcctgctac tccggaagag
     9481 ctggacattt tcgccctgaa atatcatgtg gacaaaaaca gtcctgctat catgacagtg
     9541 gcgcagataa acggcgcgga gaaatcatcc gtttcggtat tccgtgcgga tgaaagagga
     9601 gtttacaggt gggacgggct gtacacgcta tggcccggta ctcatatctt ggtagtaaaa
     9661 gaagcccgat ctacgtgatc gggctttgtt ttttaggcaa tccacacctt ttgaacatac
     9721 taaccactgc cgtgtcactt atttctcata tgcgccgtga taaaatactt ccatggtccc
     9781 gttagaagcc gcgaccgctc aaaaattcgt aatttaatat catggtcgat aaatcattac
     9841 agagaaaaat tatcgttgca gaaagcggct cggtcgcgat ctgcttctaa acgggatggc
     9901 agaatacacg atcaatacct cgagtatacg aattcctcct caaagcctcg aagccgagat
     9961 ggcgcttttg ggctccatca tgctccgccc cgaagcgatg tacgaggtgc tcgatgccgt
    10021 gagtcccgca tacttttatg ccgacaggca ccgcctcatt ttcgagacga tgcaggagct
    10081 tttcagcaag tcggtgccga tcgattttct ttcgctcagt tcgcgtctca aggagaagca
    10141 gcttctcgag caggtcggcg gcaacgtttt catcaccgag ctcgcatcgc tcgtcccctc
    10201 ttccgcgagc gtcaggcatt acgccgatat cgtccgcaag aagcacatga tgcggaagct
    10261 catcgaggcg tccgaggata ttgcgcgcat cggcttcgat gaatcggccg acctcgagga
    10321 atctcttgat gccgcggaca agaagatctt cgagatcacc aaccagggcg gatcgcacaa
    10381 gttcgtggag atgaaggata cgc
//
LOCUS       DASZYS010000055        19366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_313873, whole genome shotgun sequence.
ACCESSION   DASZYS010000055 DASZYS010000000
VERSION     DASZYS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19366
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313873"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..255
                     /locus_tag="VHF05_03215"
     CDS             109..255
                     /locus_tag="VHF05_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79966.1"
                     /translation="MEHYVCSGTCGAVSSEPGVCTTDGCTKQDEALAYCNCEDGSHSE
                     IAGK"
     gene            304..813
                     /locus_tag="VHF05_03220"
     CDS             304..813
                     /locus_tag="VHF05_03220"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HVS79967.1"
                     /translation="MGKTRRIFIIVGIAIAALICLGAAAYLFLPGLAAHEKPYAGPMK
                     TVAVFYGNSYLDPELDCTKVFPTSRQVPDTNDAMKDAILALIKGPTYDEMQKSFFTSL
                     RSDAKLQNFSVRGGVAYVDFNKRLEGELEGSCQAAGIKAEVTETLKQFLDIKDAVVTV
                     GGKNIDEIK"
     gene            complement(874..2253)
                     /locus_tag="VHF05_03225"
     CDS             complement(874..2253)
                     /locus_tag="VHF05_03225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HVS79968.1"
                     /translation="MSIEGFHQKSPEEIASQEEEARLQLELEGHAAESPYEEKLEALR
                     TVRREGKNMQSLKAEISEKVDTAIEHAIVEGKFIGSGKDAVVFKVADETFSPEMLPIL
                     VEEGFKFNTERQESLSAAAKILKIYEPGLGKREYEMQREAFQILSQSEKAAQVPTPIG
                     LRDQELDEKNKVFLNGFGTHLKGNMEVIVMDYVDGKDLATIMYEFVLEAKGYDKEALE
                     KMEFEDKANLVAKYLSFSIPSGKDRDENARAFELAATMNDNSRKLMKYLKQKGFKLDK
                     IVVDKIENALKILSRHGLHHNDMHERNIMIEGDKVFIIDFGRAVKGETDENTDDFAAV
                     RRWKELTTTPEEDEKSMHIEMISELNQKENMLLKNPKWLEKYAKIKQAIADGNTLRYA
                     NEFVMAQSSESGVEDFLILTKNLLHDSMINTNARESVQNFLHSLHSGKIPVYAKNKIS
                     NVMHSGWLQ"
     gene            complement(2266..3423)
                     /locus_tag="VHF05_03230"
     CDS             complement(2266..3423)
                     /locus_tag="VHF05_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVS79969.1"
                     /translation="MSTHKQSLIPVYAANLFLSLHYAVLLYINSSYLGTFVSEKTLSF
                     LYIAAAILNIAIFTNAARILQKISLRKLLFTVAFTDLAATASLSFMSGKIIVPTLFVL
                     LSSCSLATLFCLDIFLESRSEDKRTGRIRSGYLTLMNFAVLVSPFFSGVIVGPDSYWR
                     AYAVSAIFLAVMIAIAAAYLTDDVHPSRKLGKTFETIGLFLRDSAIRKIFTANFLLQF
                     FYAWMVVYVPIYLHEYLAFDWKTIGVIFTVMLVPFVLFEMPVGRLADTVTGEKEFLIA
                     GFVIMIAFTALIPFVPQWQLLLWAFVLFMTRVGASFVEVTTESYFWKHVNARQSGFIG
                     IFRLAEPLAYVLGPLVAIIALSLIPFQYIFLILALILLLGLGVSFALQDTR"
     gene            complement(3509..3976)
                     /locus_tag="VHF05_03235"
     CDS             complement(3509..3976)
                     /locus_tag="VHF05_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79970.1"
                     /translation="MRKRLLNSHDPALHHLAPDQLMIDDNAYRVHVNELLSKSASLFI
                     HTMPVVLQEPKYVLEDHENRDISSYIMLVKLPIVAGSADTVIHRIKIRSARRYIAQQM
                     HDMLVQEFLRIGGQMWPGEVERFIQKHRLDNILVLLENPPPQEPAPLQAAEVL"
     gene            complement(4195..6171)
                     /locus_tag="VHF05_03240"
     CDS             complement(4195..6171)
                     /locus_tag="VHF05_03240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HVS79971.1"
                     /translation="MNPQPNNGRGGGRRRFPRRNLKANMFSAVSKNGNADTRNRKEGG
                     DAGSRRAPGQLAPRNAHSRRSGGGQRNRRLRLPGRDRMPRPAAPRRKPEAKLPPLAPD
                     STRIIPLGGVEEVGKNMTALEFGNDIIVMDAGFAFHDDDTPGVDYILPNTAYLEENKD
                     RIRAVFITHGHLDHIGGIPYIMNRIGNPPLYTRSLTTIMIKKRQDEFPHLPPLDIRVV
                     EKESTVRVGSLKVSFFNVTHTIPDAMGIVIETPYGAIVNPGDIKLEHEDGVVSEREER
                     EFGRFKDMNVLLLMMDSTNVDNPGFSTPEKLVHKNLDEIIRSTKNRLIIGTFASQIER
                     MIAIIESAERANKKVVVEGRSMKNNIEIVRQTGMLKVKKETIITAEEMDNYPPDRIIV
                     LATGAQGDEFAALMRMSMKTHKYFKISKRDTVLLSASIIPGNERAVQKLKDNLARQGA
                     KIIHYRTSEVYIHSTGHGNRGELEWIHRHVKPKFFMPMHGTHYMLRVHEDLAKTIGMP
                     EDHIIVPDNGAVLEIQDGGTKFVRLKEAVASGPVMVDGFAIGDVQEVVMRDRKMLAED
                     GMFVIIASINLKTGKLKKSPDIISRGFVYLRESQELLNHARGIIKKTIEDTTAGMNPI
                     NFDFVKSNLSDAVSRYLFQKTAKRPIVIPVVLGV"
     gene            complement(6317..8098)
                     /locus_tag="VHF05_03245"
     CDS             complement(6317..8098)
                     /locus_tag="VHF05_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVS79972.1"
                     /translation="MKKPKSINSIFEKRWKLPTASKAAEAVSAFSTSERIIFFAVVAV
                     FIVSALSLLWKVNSAFLVTVPKRGGTLQEGVIGSPRFINPLLAISDADEDLTSLVYSG
                     LMKKNADGTFAPDLAESYQVSPDGLTYTFTLKPGLKFQDGTPITADDVAFTVGKAQDA
                     TLKSPKLVDWEGVEVSVLSPSQIQFTLQKPYAPFIANTTMGVLPKHIWENISSDEFAF
                     SNYNVEPVGSGPYEVKSVKRDSGGIPVYYRLSPFSGYAGAEPYIGNLVIKFFHDEDSL
                     VKAYKSHSIDSMHGIAPETAASLDDGAKIVAADLSRVFGIFFNQNQQTLFTDAKVRQA
                     LDESLDKQEIVDTVLKGYGTPLDGPVPPGLIAGATTSDAVAKSTTTPEERIAAAKSIL
                     ESDGWKLGEDGVYEKSSSNGGSQRLAFSISTTDAPELKDAANAVADQWKKLGADVTVH
                     IYPLSDLNQNVIRARKYDALLFGQAIGPDLDFYPFWHSSQRNDPGLNVAEYANTETDK
                     LLEDARATTDDAERTQDIEQFDGIIKSETPAVFLYSPSFIYVLPPWLHNATFGEISEQ
                     ADRFVTEPSWYIETNRVWQVFAPQVQK"
     gene            complement(8213..8446)
                     /gene="secG"
                     /locus_tag="VHF05_03250"
     CDS             complement(8213..8446)
                     /gene="secG"
                     /locus_tag="VHF05_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVS79973.1"
                     /translation="MSSIPGILPYIQIILSALLTGSILIQQSEAGLGGAFGGDSFSTT
                     HHTRRGFERTIFIWTIVLSVLFALSTFLVLITQ"
     gene            8637..8975
                     /locus_tag="VHF05_03255"
     CDS             8637..8975
                     /locus_tag="VHF05_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVS79974.1"
                     /translation="MAKRTSKNSASGASKKGKITPLGDRVLVRPASDESTQTASGIYI
                     PDTVDKEKPEQGTVIAVGPGRFVDGKRRPVDVAVGDLVVFSKYGYDEVKVGGEEYYLI
                     KEDNILAIIK"
     gene            9086..10741
                     /gene="groL"
                     /locus_tag="VHF05_03260"
     CDS             9086..10741
                     /gene="groL"
                     /locus_tag="VHF05_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HVS79975.1"
                     /translation="MAKQILYGEDARKALKRGIDKVADAVKITIGPRGRNVAFDKGYG
                     APTVTNDGVSIAKEVSLKDKFENMGAEIVKEVASKTNDAAGDGTTTSVVLVHAMIEQG
                     LRKIALGVNAMGVRRGIEAAASDAVEALKSMAKPVKGKEEIKQVATISAESAEIGSII
                     ADTIEKVGKDGVVTVEESQGFGIDYEVVEGLEFDKGYASPYFITDPARMEAEYRDPAI
                     VVTDKKISGIKEVLPFLEKIVQAGKKDLVIIADDIEGEALSTFILNKLRGGLNVLGIK
                     APGYGDRKKEMLQDIAATVGAAVITSDTGVTFENADMKALGSARRVVSTKDSTVIVGG
                     KGKKSEIEARVSQLKAQLGQTTSKFDKEKLEERIAKLTGGVAVIRVGAATETEMKYLK
                     LKIEDAVAATKAAIDEGIVVGGGSALVKVAQKIEAKSSKKDDKGTEYAAGYSVVVDSL
                     LAPMKQIVLNTGSDRGGAVIDKIMAGEGKSQNAGYDAQNETIVSDMFAAGIIDPVKVT
                     RTALQNAASAAAIFLTTEVAIADEPEEKKGGPAMPPGMGGMDY"
     gene            11014..11160
                     /locus_tag="VHF05_03265"
     CDS             11014..11160
                     /locus_tag="VHF05_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79976.1"
                     /translation="MKHHSHSAHPSHHRRGNSGLMDALEKIFPILLACLGAIVILVLA
                     MFIR"
     gene            11227..12390
                     /locus_tag="VHF05_03270"
     CDS             11227..12390
                     /locus_tag="VHF05_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79977.1"
                     /translation="MAEAKVGRSRLPLIIISGTLIFIAAAVWILSRSATYNAGFSLNG
                     ITPVFVKGVVVKNVTNTSDLPVGGTAYLEILDPDNSIQTVYYSNGVEDCSDDAKAIQS
                     GEKVSVYGLPLGNGEYTLCGSSYFKIESLGATGPYAGWSEYEDTAAGFSFKFPGNWTL
                     STSSRSAGVEVDLFPPKAADPALMVVSTGGLGNLRLEDWAQSFYRISPADASYATVGG
                     AEAMVFPASQNNAQSVMLLKHGDYVLEFDFASADANVPKELVAIPALIMKSVLLERVA
                     SSTASSSGELKTGLRGAVTISPVCPVEKNPPDPMCAPKPYQAKLRVVAQDGTVVKRFI
                     TDAQGAYSVDLDPGIYTVENDASSALPSLAPQRVAVVDHSLSVLNLQFDSGIR"
     gene            12688..13248
                     /locus_tag="VHF05_03275"
     CDS             12688..13248
                     /locus_tag="VHF05_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVS79978.1"
                     /translation="MVIGIIIVAIILILVLWLVFTYNGFIRLVNRAKEAWADIDVQLK
                     RRYDLIPNLVNTVKGYAAHEKEAFENVSAARSAAMGAQNLADKTQAENALSGALGRLF
                     AVAEAYPELKANQNFLDLQTQLTDTEDKIQAARRFYNGNVRDLNIKIDSFPGNIIANS
                     FHISKMEFFELSEKDSAAREPVEVKF"
     gene            13586..15289
                     /locus_tag="VHF05_03280"
     CDS             13586..15289
                     /locus_tag="VHF05_03280"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HVS79979.1"
                     /translation="MKLYKFVFTALLIGCAFIVHAQSDTSESIRSFDVAAVVNQDSTV
                     RITETITYDFGANERHGIFRDIPVKYDTTLGNQSISLKDIKVADEKGTPYEFTTSSTG
                     KDEEIKIGDPDSTVTGEKTYVISYTAERALGYFSDHDEFYWNATGNEWQVPIGEASFT
                     MTVPFVADAKNLQSSCYYGELGSAATCTPATVSMQKGTTVTFVAPSALEAGQGLTGAV
                     GFPKGLVYEPSGAAKFWQVVRDNGILALPGLVLLVMFFLWRKYGKDPKGRGTIIAEYD
                     APDGLTPLEVSAIRYQGSRQKDISAEIIRLAVLGYVKIIRTEEKHRFSKSASYTLERT
                     TPKRQATLSEAEEILLDKLFDGGTEVKLSDLNQKFYRNVPKIKKSTMNSVISKGYYAN
                     DPAKARGLYVGIGIVLVAIGIFAAFSAAWVIALALSGVIVICFGLVMSKLTEKGMEAK
                     QGILGLREYLQIAEKDRINFHDAPEKSPELFEKLLPFAMVLGVEKAWAKEFEGIYTAP
                     PAWYSDPYGGVFNPIFFANSVSAFGEAATRSLTAGPANSGAGGGGFSGGGGGGGGGGS
                     W"
     gene            15662..16150
                     /locus_tag="VHF05_03285"
     CDS             15662..16150
                     /locus_tag="VHF05_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVS79980.1"
                     /translation="MAKYPAYEIVITAIIHRDGKYLITRRSLQKKRFPGMWTVPGGHL
                     EPSDYESAPKETEHYWYNVLEKALTREVREETGVEIENVRYVTSLATTHTDGAPSLVI
                     SCLADYVSGDVKLQEEETDKAEWVSVEEAKGYELIDGIYDKIAMADKLRLGEKYEWKR
                     HG"
     gene            16199..17107
                     /locus_tag="VHF05_03290"
     CDS             16199..17107
                     /locus_tag="VHF05_03290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVS79981.1"
                     /translation="MNIYHEQASNIWKTWGLMTFFLVFFIALGWIFSEAYDSEGILVF
                     AILFSIVLNIFSYWFSDKIALKSAGAREITKEENPTYWNVVENLAITAGLPMPRLYVI
                     NDPVPNAFATGRNKEHAAVAATTGLLQMMDRTELEGVIAHELSHIGNRDILISTVVVV
                     LAGSIAMLSNIFLRSNFMFGGRRGNDRNGGGAIFLVIGLVAAILAPISATLIQLAISR
                     KREYLADASGALLTRYPEGLASALEKIEHYSGQLKHANNATAHLYISNPFGDRAHIQS
                     FMTRIFSTHPPIEDRIRILRGQEKML"
     gene            17263..17616
                     /locus_tag="VHF05_03295"
     CDS             17263..17616
                     /locus_tag="VHF05_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79982.1"
                     /translation="MQAFLVFNSVAIPIAFGSSQQETVKILISVIGFFVHVGLSITVW
                     QAIRWIRYLDDALADFEQLDTEVNSVAAVRVAIFSNVIFEQRRGGWLRSHAYLVPFGI
                     GAISWFLRIAAHFYF"
     gene            17888..18175
                     /locus_tag="VHF05_03300"
     CDS             17888..18175
                     /locus_tag="VHF05_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79983.1"
                     /translation="MTKFWFKSFIGAGGFSNMPQSSIGQSSAEADDVQALANALAKLS
                     DHYSKSEDPKILCAVDTIDVSIEMLMRDENPDGFSGIARARVGAARIMNVS"
     gene            18503..18868
                     /locus_tag="VHF05_03305"
     CDS             18503..18868
                     /locus_tag="VHF05_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79984.1"
                     /translation="MSRPNASTTQFDPYVFQIEKEISKLFVRARHIANTHEDSNLRQF
                     CRVFKAAWRRYESRKKDKSTTSQSVLGETARLLQDTINWSRTNAQEHNDPALELACGM
                     MQKALDIYKHSGRAPARST"
     gene            18965..19216
                     /locus_tag="VHF05_03310"
     CDS             18965..19216
                     /locus_tag="VHF05_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79985.1"
                     /translation="MTEEQKLIEDKIDAFIAHIQSLDIPTELKNQLRRYLRAFVSAHM
                     VCKQEELEKTMRAFLANSVKPAIRMLDEFDKLEVHSRPI"
BASE COUNT         4362 a         5025 c         5474 g         4505 t
ORIGIN      
        1 atggcgcctt cgcctatctc acggggtgtt tgtattgtgg aaaaagccgg cccggataaa
       61 ccgcgggagg gcgtataata gggaccatta gaggctaatc gtaagtacat ggaacattat
      121 gtttgcagcg gcacgtgcgg agcggtaagc tccgagccgg gcgtttgcac gaccgacgga
      181 tgcaccaagc aggacgaagc tcttgcgtac tgcaattgcg aggacggctc gcattcggaa
      241 attgccggca aataattccg ctcgggcggc ttcctcggct ctcgcatgag agcttctatc
      301 cctatgggca agacacggcg catattcatc atagtcggaa tcgcgattgc ggccctcatc
      361 tgccttggag cggcggcgta tctcttcctg ccaggattgg cggcgcatga gaagccctac
      421 gccggcccga tgaaaaccgt cgccgtcttc tacggcaatt cctatcttga cccggagctc
      481 gactgcacta aggtctttcc gacgagccgg caggtgcccg atacgaacga tgcgatgaag
      541 gacgcgatcc tcgcgctcat caaggggccg acctatgacg agatgcagaa aagcttcttc
      601 acgagcctga ggtccgacgc gaagctccaa aatttttccg ttcgcggcgg cgtcgcctat
      661 gtcgatttca ataagaggct cgaaggggag ctcgaaggtt cgtgccaggc ggcaggcatc
      721 aaggccgagg tcaccgaaac cttgaagcaa ttcctggaca tcaaggatgc agtggtaacc
      781 gtcggcggga agaatatcga tgagatcaaa taaataaaat gcctgccaga caactatctg
      841 gcaggcattt ttcctgcaag aaaatcccat agcttattga agccaaccgg aatgcattac
      901 attgctaatc ttattttttg catagacggg aattttgccg gaatgcagtg agtgtaaaaa
      961 attctgaact gattcgcgag cattggtatt tatcatggaa tcgtgcagaa gattttttgt
     1021 taggatgaga aaatcttcca ccccgctttc gctgctttgt gccatgacga attcatttgc
     1081 atacctcagc gtattgccat ccgcgattgc ctgcttgatc tttgcgtatt tttcgagcca
     1141 tttcggattc ttgagcagca tgttttcctt ttgattcagt tcgctgatca tctcaatgtg
     1201 catgcttttc tcatcttctt ccggagtggt tgtaagttct ttccatcgcc gcactgccgc
     1261 aaaatcatcc gtattttcgt ctgtttcacc ttttaccgcc cggccaaaat cgataataaa
     1321 taccttgtcg ccttcaatca tgatattgcg ttcatgcata tcgttgtgat ggaggccgtg
     1381 tcgcgaaagg atttttaagg cattttcaat tttatcaacc acgatcttat caagcttaaa
     1441 acccttctgc ttgagatatt tcataagttt cctgctgttg tcgttcatag ttgcggcaag
     1501 ttcgaaagcg cgtgcattct cgtctcggtc tttgccgctt ggaattgaaa aactgaggta
     1561 ttttgcaacc agatttgcct tgtcttcgaa ttccatcttc tcgagggcct ccttgtcata
     1621 tccttttgct tccaggacga attcgtacat gatcgtggcg agatcttttc cgtcgacgta
     1681 atccatcacg attacttcca tgttgccctt aagatgcgta ccgaagccgt tgagaaatac
     1741 cttgtttttc tcgtcgagtt cctgatctct gagtccgata ggtgtgggga cttgtgctgc
     1801 tttttcagat tgtgaaagaa tctgaaacgc ttctcgctgc atttcgtact cgcgcttgcc
     1861 taatccgggt tcataaattt tgagaatttt tgctgcggca gataaagact cttgtctttc
     1921 ggtattaaat ttaaatcctt cttctacaag gatagggagc atttccggcg agaatgtctc
     1981 atcggcgact ttgaaaacaa ccgcgtcttt tcctgagccg atgaatttgc cttctacaat
     2041 cgcatgttca atggcagtat cgactttctc gcttatctct gctttgagag attgcatgtt
     2101 ctttccttct cgtcgaaccg ttcttaaagc ttcaagtttt tcttcgtatg gactttcagc
     2161 cgcatgtcct tcgagttcaa gctgaagacg cgcttcctcc tcctgtgaag ctatttcttc
     2221 ggggcttttt tgatggaaac cttcaatact cattctctaa ctctactatc tcgtatcctg
     2281 caaggcaaag gagacgccga ggccgaggag aaggatgagg gcgaggatga gaaagatgta
     2341 ctggaaggga ataagggaaa gggcgatgat ggcgacgagc gggcccaaga cgtacgctaa
     2401 gggctccgcg aggcggaaaa tgccgatgaa gcccgactgc ctggcgttca cgtgcttcca
     2461 gaagtagctc tcggtcgtca cctcgacgaa gcttgcgccg acgcgggtca tgaagaggac
     2521 gaacgcccat aggaggagct gccattgcgg gacgaagggg atgagcgcgg taaaggcgat
     2581 catgatgacg aagccagcaa tgaggaattc cttttctccc gtcacggtgt cggcgaggcg
     2641 gcccaccggc atttcgaaga gcacgaaggg aacgagcatc acggtgaata tgacgccaat
     2701 cgtcttccag tcgaaagcca gatattcatg gaggtagatc ggcacgtaga cgaccatcca
     2761 ggcatagaag aactggagaa ggaagttcgc agtgaagatt tttcggatgg cgctgtcgcg
     2821 gaggaagagg cctattgttt cgaaagtctt gccgagcttg cgcgagggat ggacgtcgtc
     2881 cgtgaggtac gcggcggcga tggcgatcat gacggcgagg aagatcgccg agaccgcata
     2941 cgcccgccaa tacgaatcgg ggccgacgat gactcccgaa aagaacggcg agacgaggac
     3001 ggcgaaattc atgagagtga gatagccgct tctgatccgg cccgtgcgct tgtcctccga
     3061 gcggctctcg agaaagatgt cgaggcagaa gagcgtcgcg agcgagcacg acgaaaggag
     3121 gacgaagagg gtcggcacga ttatcttgcc gctcatgaag cttaaggagg cggtggccgc
     3181 gagatcggtg aaggcgacgg tgaaaaggag cttccgcagg cttatcttct gaaggatcct
     3241 tgcggcattg gtgaatatgg cgatattcaa gattgccgcc gcgatataga gaaagctcag
     3301 cgttttttcc gagacgaacg tgccgagata ggaggaattg atatagagaa gcaccgcgta
     3361 gtggagcgac aggaagagat tggcggcata tacgggaatg aggctctgct tgtgcgtact
     3421 catgaaattc cattgtatca gattttcacg gttgcgaatt cacaaaaaaa gccgcacacg
     3481 gtacgtaagg tacgtgagcg gccttgagtc agagaacctc tgcggcttgt agcggcgcgg
     3541 gttcctgcgg gggcgggttt tcgagaagga caagtatgtt gtcgagtctg tgcttttgta
     3601 tgaatctctc aacttcgcca ggccacattt gcccgccgat gcggaggaat tcttgtacca
     3661 gcatgtcgtg catttgctgt gcaatatagc gccgcgcgct ccgtatcttg atgcggtgga
     3721 ttactgtgtc ggcgcttccg gcgacgatgg gaagcttgac gagcatgata tacgaagata
     3781 tgtcgcgatt ctcatggtct tcaagtacgt acttcggttc ctgcaatacg acaggcattg
     3841 tatggatgaa aaggcttgcc gatttcgaga gcagttcgtt gacatgaacc ctataggcat
     3901 tgtcatcgat catcaattgg tcgggtgcaa gatgatgcag agccgggtcg tggctgttga
     3961 gcaatctctt ccgcattgct tcatattctg caagtttgcg gatgtccttt acatcattga
     4021 atttggtccg agataaatag cttcgaaatt ccaattcagt tctccttgga aagctgttcc
     4081 gaaataaacg tagcaccttt tgttttgcat gtaaacaaaa atcgctcata tcctggtgtg
     4141 agcgattttt ttcttctttt ctaaaagcta taagctgtct tgctacaagc ttctctacac
     4201 tcccagcacc accggaatga cgatcgggcg cttggcggtc ttctggaaga ggtagcggct
     4261 gacggcatcc gagagattcg atttcacgaa gtcgaagttg atcgggttca tgccggcggt
     4321 cgtgtcctcg atggtcttct tgatgatgcc gcgggcgtgg ttcagaagct cctgcgactc
     4381 gcggagatag acgaagccgc gcgagatgat gtcaggggat ttcttgagct tgccggtctt
     4441 caagttgatc gacgcgataa tgacgaacat gccgtcctcg gcgagcatct tgcggtcgcg
     4501 catgacgact tcctggacgt cgccgatcgc gaagccgtcg accatgacgg ggccggatgc
     4561 gaccgcctcc ttgaggcgca cgaactttgt gccgccgtcc tggatctcga gcaccgcgcc
     4621 attgtcggga acgatgatat ggtcctcggg cataccgatc gtcttggcaa ggtcctcatg
     4681 gacgcggagc atgtagtgcg tgccgtgcat cggcatgaag aacttcggct tcacatggcg
     4741 gtggatccat tcgagttcgc cgcggttgcc gtggccggtc gagtggatgt agacctccga
     4801 ggtgcggtag tggatgatct tggcgccttg gcgggcgaga ttgtccttga gcttctgcac
     4861 cgcgcgctcg ttgcccggga tgatcgaggc cgaaaggagc acggtgtcgc gcttcgaaat
     4921 cttgaaatac ttgtgggtct tcatcgacat gcgcatgagt gccgcgaact cgtcgccctg
     4981 ggcgccggtc gcgagcacga tgatcctgtc gggagggtag ttgtccattt cctcggcggt
     5041 gatgatcgtc tccttcttga ccttgagcat gccggtctgg cggacaatct cgatgttgtt
     5101 cttcatgctg cggccctcga cgacgacttt cttgttggcg cgctcggccg actcgatgat
     5161 cgcaatcatg cgctcgatct gcgacgcgaa ggtgccgatg atgaggcggt tctttgtgga
     5221 gcggatgatc tcgtcgagat tcttgtggac gagcttttcc ggagtcgaga agcccggatt
     5281 atcgacgttt gtcgaatcca tcatgagaag gagcacgttc atgtccttga atcttccgaa
     5341 ctccctttcc tcgcgctccg agacgacgcc gtcctcatgc tcgagcttga tgtcgcccgg
     5401 gttcacgatc gcgccgtacg gcgtctcgat gacgatgccc atggcgtcgg ggatggtgtg
     5461 ggtgacgttg aagaagctca ccttgagcga tccgacgcga acggtcgatt ctttttcgac
     5521 cacgcggatg tcgagaggcg gcagatgggg gaattcgtcc tggcgcttct tgatcatgat
     5581 ggtcgtgagc gagcgcgtgt agagcggcgg gttgccgatg cggttcatga tatagggaat
     5641 gccgccgata tggtcgaggt ggccgtgggt gatgaagacc gcgcggatgc ggtccttgtt
     5701 ttcctcgagg tacgcggtgt tgggaaggat gtagtcgacg cccggcgtgt cgtcgtcgtg
     5761 gaacgcgaag cctgcgtcca tgacaatgat gtcattgccg aactcgagcg cggtcatgtt
     5821 cttgccgact tcctcgacgc cgcccaaggg aatgatgcgg gtgctgtcgg gagcgagggg
     5881 agggagcttc gcctcgggct tcctgcgggg agccgccggg cggggcatcc tgtcccgccc
     5941 ggggaggcgg agccgcctgt tgcgctgtcc gccgccggag cgcctcgaat gcgcgttgcg
     6001 cggcgcgagc tgtccgggcg cgcggcggga tccggcgtcg ccgccttctt tcctgttgcg
     6061 agtgtccgcg tttccgttct tgctgacggc ggagaacatg tttgccttga ggttccttct
     6121 cgggaatctc cgcctgccgc ctccgcggcc gttattgggt tgggggttca taagtaattc
     6181 tgtgttggta attaataata atgattctta agtttgagaa ttttcaattt ttaattttca
     6241 atcaagccgc aatgaccaat tttcaaggtt tgagtattga taaattgaaa attgactgaa
     6301 aattgagaat tggaaattat ttttgcactt gcggcgcgaa aacctgccat acccggtttg
     6361 tctcgatgta ccagctcggc tccgtgacaa aacgatccgc ttgctccgag atttcgccga
     6421 acgttgcgtt gtggagccag gggggaagga cgtaaatgaa cgagggggaa tagaggaaca
     6481 ctgccggggt ctcgctcttg atgatgccgt cgaactgctc gatatcctgc gttcgctccg
     6541 catcgtcggt ggtcgcgcgc gcgtcttcga gaagcttgtc ggtctcggtg ttcgcgtact
     6601 ccgcgacgtt gagccccgga tcgttccttt gcgacgagtg ccagaacgga tagaagtcga
     6661 gatcggggcc gatcgcctgc ccgaagagca gggcgtcgta cttgcgtgcg cggatgacgt
     6721 tctggttcaa gtcggagagc gggtagatgt gaactgtcac gtcggcgccg agcttcttcc
     6781 actggtccgc cacggcattc gccgcgtcct tgagctccgg cgcgtcggtc gtcgagatgc
     6841 tgaacgcgag gcgctgcgat ccgccgttcg atgaggactt ctcatagacg ccgtcctctc
     6901 cgagcttcca gccgtcgctt tcgagaatgc ttttcgcggc ggcgatgcgc tcctcgggag
     6961 tcgtcgtgct ctttgccacc gcatccgacg tcgtcgctcc cgcaatgagg cccggcggaa
     7021 cagggccgtc gagcggcgtg ccgtatccct tgagcaccgt atcgacgatc tcctgcttgt
     7081 cgaggctctc gtcgagcgcc tggcgcacct tcgcgtcggt gaagagcgtc tgctggttct
     7141 ggttgaagaa gatgccgaag acccgcgaaa ggtccgccgc cacgatcttc gcgccatcat
     7201 cgagcgaggc ggcggtttcc ggcgcgatgc cgtgcatgct gtcgatcgag tgcgacttgt
     7261 acgccttcac cagggaatcc tcgtcatgga agaacttaat gacgagattt ccgatgtacg
     7321 gctccgcacc ggcgtacccg gagaacggcg agaggcggta gtagacggga atgccccccg
     7381 aatcgcgctt cacgctcttc acttcatacg gccccgagcc caccggttcg acgttgtaat
     7441 tgctgaatgc gaattcgtcg gagctgatat tctcccagat gtgcttcggc aggacgccca
     7501 tcgtcgtgtt tgcgatgaac ggcgcgtacg gcttctgcag ggtgaactgg atctggctcg
     7561 gcgaaagcac cgacacctcg acgccctccc agtcgacgag cttcgggctc ttgagcgtag
     7621 cgtcctgcgc cttgcctacc gtgaacgcca cgtcgtcggc cgtgatcgga gtcccgtcct
     7681 ggaacttcag ccccggcttc agggtgaagg tgtaggtgag gccgtccggc gagacctggt
     7741 agctttccgc aagatccggc gcgaacgtgc cgtcggcgtt ctttttcatg aggcccgaat
     7801 agaccaggga cgtgaggtcc tcgtccgcat cgctaatcgc gagcagggga ttgatgaagc
     7861 ggggagagcc gatcactcct tcctggagcg ttccgccccg tttcggcact gtgaccagaa
     7921 acgcgctgtt caccttccag agaagggaaa gcgccgaaac gatgaagacc gcgacgaccg
     7981 cgaagaagat gatccgctcg cttgtcgaga aggcagaaac ggcctcggcc gccttggaag
     8041 ccgtggggag cttccagcgc ttctcgaaaa tgctatttat tgattttggt tttttcacga
     8101 ggatgggcgt ggacgcagtg cgtccgcacg aatgccggcc gagataaccg cagaattacc
     8161 gagaatgtcc gcaataacgg aggcggtccg aaccgtcttt aggtttgtag tgctattggg
     8221 tgatgagtac caggaatgtc gagagggcga agagcaccga aaggacgatc gtccagatga
     8281 agatggtgcg ttcgaaaccc ctccgcgtgt ggtgggtggt gctgaaactg tcgcctccga
     8341 aggcgccgcc gaggcctgct tccgactgct ggatgagaat cgagccggtc agaagggctg
     8401 aaaggatgat ctgtatgtac ggtaatatcc cgggaatact gctcattgtg gcgattatac
     8461 cagcaatttc cgaagttgca aggagggttt gtccacggtt ccgccgtaga tttttctccg
     8521 ttcctgcgcg gaggagggct gccctaaggg cattttgact ctctcggatg tctccactat
     8581 aattttaggc gcaattattt aagattaatt atcagcttca tctaatcata cgagctatgg
     8641 caaaacgcac atcgaaaaat agcgcttctg gcgcttcgaa gaaagggaag atcacgcctt
     8701 tgggcgaccg ggtgctcgtg cgtccggctt ccgacgaaag cacgcagacg gcatcgggca
     8761 tctacattcc cgatacggtg gataaggaga agcccgaaca gggcactgtt attgccgtcg
     8821 gccccggacg cttcgtcgac ggcaagcgcc gtccggtgga cgtcgctgtc ggcgacctcg
     8881 tagtgttttc caaatacggc tacgacgaag tgaaggtcgg cggcgaggaa tactatctca
     8941 ttaaggaaga caatattctt gcgatcataa aatgatcgca agaagtcaaa atgcaaaaat
     9001 caaaagtcaa aattataatt cataactttt aattttcgga aattttgatt tttgaattca
     9061 attaacatct aacgatacaa gtaatatggc caagcaaata ctttatggcg aagacgctcg
     9121 gaaggctttg aagcgcggca tcgacaaggt ggccgacgca gtgaagatca ctatcggccc
     9181 gcgcgggcgc aacgtcgcgt tcgacaaggg ctatggcgct ccgactgtta ccaacgacgg
     9241 cgtctcgatc gccaaggaag tttccctcaa agataagttc gagaacatgg gcgccgagat
     9301 cgtgaaggaa gtggcgtcga agacgaacga cgcggctggc gacggcacga ccacctcggt
     9361 cgtcctggtg cacgccatga tcgagcaggg cttgcggaag atcgccctcg gcgtcaacgc
     9421 catgggcgtg cgccgcggca tcgaggcggc cgccagcgac gcggtggaag cattgaagtc
     9481 gatggcaaag ccggtcaaag gcaaggagga gatcaagcag gtcgcgacaa tttccgctga
     9541 atccgcggag atcggttcga tcatcgccga caccatcgag aaggtgggca aggacggcgt
     9601 cgtgaccgtc gaggaatccc agggcttcgg cattgactac gaagtcgtcg aaggcctcga
     9661 gttcgacaag ggctacgcct ccccgtattt catcaccgat ccagcccgca tggaagccga
     9721 gtaccgcgac cccgccatcg tcgttaccga caagaagatt tcggggatca aggaagtgct
     9781 cccgttcctg gagaaaatcg tccaggcggg gaagaaggat ctcgtcatca tcgccgacga
     9841 catcgaaggc gaggcgctct ccacgttcat actcaataag ctccgcggcg ggctgaatgt
     9901 cttgggaatc aaggctcccg gctatggcga ccgcaagaag gaaatgctcc aggatatcgc
     9961 cgcgaccgtc ggcgccgctg ttatcacctc cgataccggc gttacgttcg agaatgccga
    10021 catgaaagct cttggctcgg cgcgccgcgt cgtctcgacc aaggacagca cagtcattgt
    10081 cggcggcaag ggaaagaagt ctgagattga ggcgagagtg agccagctca aggcccagct
    10141 cggccagacg acctcgaagt tcgacaagga aaagctcgag gagcgcatcg ccaagctcac
    10201 gggcggcgtc gcagtcatcc gcgtcggcgc ggcgaccgag acggaaatga aatatttgaa
    10261 attgaagatc gaggacgccg tcgcggcgac caaggcggcg atcgacgagg gtatcgtggt
    10321 gggcggcgga agcgcgctcg ttaaagtcgc gcagaagatc gaagcgaagt cctccaagaa
    10381 ggatgacaag ggaaccgagt acgcggcggg ctactccgtt gtcgtcgact cgctcctcgc
    10441 tccgatgaag cagatcgtct tgaacacggg aagcgaccgg ggcggcgccg tgatcgacaa
    10501 gatcatggcc ggagaaggca agtcgcagaa cgccggctac gacgctcaga acgaaacgat
    10561 cgtttccgac atgttcgcgg cgggaatcat cgacccggtg aaggtcaccc gcaccgcgct
    10621 ccagaacgcc gcgtcggccg ccgcgatctt cctcacgacc gaagtcgcga ttgccgacga
    10681 gccggaagag aagaagggcg gcccggcaat gcctccggga atgggcggaa tggactacta
    10741 aaagaagtag atagtaacta gtagttagac aagagcagaa aatagaaaag agcgcttcgg
    10801 cgctctttgt catgcccagc agggcatcca aggcttctgt cattgcgagt ctctcgaagc
    10861 aatccgcttt atgctaaaag attatggaat cctttttctc ctgtaagctg tcaggctata
    10921 aactattagc tctcttatac acacctcttc atagattgtt gcttgatact gtgtgataat
    10981 gatggggtaa cattaggtcc aattgttttt atcatgaagc accattctca ttccgcgcac
    11041 ccgtcgcatc atcgtcgcgg caactcggga ctcatggacg ctctcgagaa gatctttccg
    11101 attctcctcg catgcctcgg agcgatcgtc atcctcgtcc tcgcgatgtt catccgctag
    11161 gctcgcgtct tcaaataatg ccgcttccca ttctgatgcg gagcggtatt tttgtataat
    11221 ggtggcatgg cagaggcaaa ggtcgggcgt tcgcgcttgc cgcttatcat catttccgga
    11281 actctcattt ttatcgccgc ggcggtgtgg attctgtcgc gcagcgctac atacaatgcg
    11341 ggcttcagct taaacggcat cacgcccgtc ttcgtgaaag gcgtggtcgt gaagaatgtc
    11401 acaaatacct cagatcttcc cgtcggcggc acggcgtatt tggaaattct cgatcccgac
    11461 aacagtatcc aaaccgtcta ttattcgaac ggcgtagagg attgctcgga cgacgctaag
    11521 gctatccaaa gcggcgagaa ggtgagcgtc tatgggctac cgctcggcaa tggggaatat
    11581 acgctctgcg gttcgagcta tttcaagatt gaaagcctcg gcgctacggg cccctatgcg
    11641 gggtggagcg aatacgaaga tactgccgcg gggttctctt tcaaatttcc gggaaactgg
    11701 acgctttcga cgtcgagccg gtccgccgga gtcgaggtgg atctttttcc gccgaaagcg
    11761 gccgatccgg cgctcatggt cgtttcgaca ggcggcctcg gcaacctccg cctcgaggac
    11821 tgggcgcaga gtttctaccg catctctccc gccgatgcga gctatgccac ggtcggcggc
    11881 gcggaagcga tggtttttcc cgcttcgcag aacaatgctc aaagcgtcat gctcctcaag
    11941 cacggcgatt acgttcttga attcgacttc gcatccgctg atgcgaacgt tccgaaagag
    12001 ctcgtagcta ttcccgcgct cattatgaaa agcgtacttc tcgaacgtgt cgcctcgtcg
    12061 acggcttctt cctccggcga attgaagacg gggctcaggg gcgccgtcac catctctccg
    12121 gtgtgccctg tcgaaaagaa tccgcccgat ccgatgtgcg ctcccaagcc gtaccaggcg
    12181 aagctccgcg ttgttgccca agacggcacg gtcgtgaagc gcttcatcac cgatgcccaa
    12241 ggcgcctact ccgtcgatct tgatccgggc atctacaccg tcgagaatga cgcgagctcg
    12301 gcactgccga gcctcgcgcc acagcgggtc gccgtagtgg atcattcgct ttcggtgctc
    12361 aatcttcagt tcgatagcgg tatccgctag gactgccatt tggaaaagca cggatatttt
    12421 tctgctaaaa aacttcttac tgaagctgta caccttttcg ctctcgaaga ctcaagctga
    12481 aaaggtcttg cgcacgctgc gcttgctcgc gtgctcacct cgagttcgcg ccagtcgcgc
    12541 tcgctagtct tttccaaatg gcagtccgtc gctccagtgt tctgaaggta aagccggaag
    12601 accggcttta cattattttt ttgctccttc tattttttta aaattgttgt ataatcataa
    12661 aacttacgaa attaattttt tgaaactatg gtcattggaa tcatcattgt agctatcatc
    12721 cttatcctgg tcctgtggct cgttttcacc tataacggct ttatcaggct cgtaaaccgc
    12781 gcgaaggagg catgggcgga tatcgacgtc cagctgaagc gccgctacga cctcattccc
    12841 aacctcgtga acacggtgaa gggctatgcc gcgcatgaaa aggaggcgtt cgagaacgtc
    12901 tcggcggccc gcagcgcggc catgggcgcg cagaatctcg ccgacaagac gcaggccgaa
    12961 aacgcgcttt caggcgcgct cggcaggctc ttcgcggtcg cggaggcata cccggaactc
    13021 aaggcgaacc agaacttcct cgatctccag acccagctca ccgacaccga agacaagatt
    13081 caggcggcgc gccgcttcta caacggcaac gtccgcgact tgaatatcaa gatcgactcg
    13141 ttcccaggca acatcatcgc caattcgttc catatctcga agatggaatt tttcgaattg
    13201 agtgaaaaag actcagccgc tcgcgaaccc gtggaagtga aattctgatc aatgctcaat
    13261 cgactactta tctcgatagg gattacggta gtaagttata tagccagtct gttttcgctg
    13321 tttctcggaa tcggattggc tgattcgggg catattccct atgtgatcga ggtgctttgg
    13381 acgtcattca tgtatattat cggattcggg atagttgtcg ccggtaatat tttgcagaca
    13441 aattcattca tcattcagat ccttattcaa ttcgcatata tctttattat tgtggaagct
    13501 tttggcagag gaattgaatg gtggcagaga aaaaggaaca atatttaatc ctattatctg
    13561 ctagtaagac gattgaacct aaaaaatgaa actatataaa ttcgtattca ccgcgcttct
    13621 catcggctgc gccttcattg tccatgccca aagcgacacg tccgagtcga tccgatcctt
    13681 cgacgtcgcc gcagtcgtca accaggattc gactgtgcgt attaccgaaa ccattaccta
    13741 cgatttcggc gcgaacgagc gccatggcat cttccgcgac attccggtga agtacgatac
    13801 gacgctcgga aaccagtcga tctcgctcaa ggacatcaag gtggccgacg agaaaggcac
    13861 gccatacgag tttacgacga gctcgacagg caaggacgag gagattaaaa tcggcgatcc
    13921 cgactcgacc gtgacgggcg agaagaccta cgtcatctcc tacaccgcgg agcgtgcgct
    13981 gggctacttt tccgaccacg acgaattcta ttggaacgcg actggaaacg agtggcaggt
    14041 gccgatcggg gaagcgagct tcaccatgac ggtgccgttc gtcgcggatg ccaaaaatct
    14101 ccaatcatcc tgctactacg gcgagctcgg cagcgccgcg acctgcacgc ccgcgactgt
    14161 ctcgatgcag aagggaacga cggttacgtt cgtcgcgccg agcgccctcg aagcggggca
    14221 agggctcacc ggcgcggtcg gatttcccaa gggcctcgtc tacgagccgt cgggcgccgc
    14281 gaagttctgg caggtggtcc gcgacaatgg catcctcgcg cttccgggtc tcgtgcttct
    14341 cgtgatgttt ttcctctggc gcaagtacgg caaggatccg aagggccgcg gtacgataat
    14401 tgccgagtat gacgcgcctg acggcctcac gccgctcgaa gtatcagcga tccgctacca
    14461 gggttcgagg cagaaagaca tttccgccga gatcatccgg ctcgccgttt tggggtatgt
    14521 gaaaattatc cgcactgaag agaagcatcg tttctcgaaa agcgcgagct acacgctcga
    14581 gcgtacgacg ccgaaaaggc aggccacgct ctccgaagcc gaagaaatcc tcctcgacaa
    14641 gcttttcgac ggcgggaccg aagtgaagct ctccgatctc aatcagaagt tctatcgcaa
    14701 cgtcccgaag atcaagaagt cgacgatgaa ctcggtcatt tcgaaaggct actacgcgaa
    14761 cgatccggcg aaggcgcgcg ggctctacgt cggaatcggc atcgtgctcg tggcgatcgg
    14821 cattttcgct gcattttccg ccgcctgggt gatcgctctc gcgctttcgg gcgtcatcgt
    14881 catctgcttc ggactcgtca tgtcgaagct cacggaaaag gggatggaag cgaagcaggg
    14941 aatcttaggc cttcgcgaat atcttcagat cgccgagaaa gatcgcatca attttcacga
    15001 cgctcccgag aagagcccgg agcttttcga aaagcttttg ccgttcgcca tggtgctcgg
    15061 cgtcgagaag gcctgggcca aggaattcga aggcatctac accgcgccgc cagcctggta
    15121 cagtgatccg tatggcggag tgttcaatcc catatttttt gcaaatagcg tctcggcatt
    15181 cggcgaagcg gcgactcgct cgcttaccgc aggtcctgcc aattccggcg ccggcggcgg
    15241 aggattcagt ggaggaggcg gaggcggcgg cgggggaggg agttggtaac aactcccaat
    15301 cacccgcctc gccttcctta tggtggaggg ggtgagcttg ggggtggggt gagctgcgac
    15361 tactggaagc agcgcaagac cttttgagat attttcgagt gatacgaaga aaatatccaa
    15421 aaggtgtacc gtttctgtaa gaaaccaagt gcaaggccgg agcgcgactg gcgcgaggtg
    15481 agaagcacgt tagggaaccc gcaattctga ttgcgggcga gcgcttcgca agggcggagg
    15541 ccgacgggcc gagccgtggt caggcgattt ttcagcagaa aaatacacgt gacgcggagg
    15601 agttttatag taaatcgctc tccattgtac aattaaccta ttagaaccct cattcaaacc
    15661 catggcgaaa tatcctgcgt acgaaattgt aataactgct atcatccacc gcgacggcaa
    15721 gtacctcatt acccgccgct cattgcagaa gaagcgcttt cccggcatgt ggacagttcc
    15781 gggcggccat ctcgagcctt ccgactacga atcggcgccc aaggagactg agcattactg
    15841 gtacaacgtg ttggagaaag cccttacccg cgaagtgcgc gaggagacgg gagtcgagat
    15901 cgagaatgtc cgctacgtga cgagccttgc taccacgcac acggacggag cgccgtcgct
    15961 cgtgatttcc tgccttgccg attatgtgtc cggcgacgtg aagctccagg aagaggagac
    16021 cgataaggcg gagtgggtga gcgttgagga agcgaaggga tacgaactca tcgacggcat
    16081 ctacgacaag atcgcgatgg ccgataagct ccgtctcggc gagaagtacg aatggaagcg
    16141 ccacggataa ttcaccgaat tctaatgatt gcggcgtaaa gaaactgcta tggcaacgat
    16201 gaatatatat cacgaacagg catcgaatat ctggaagacc tgggggctca tgaccttttt
    16261 cctcgtcttt ttcatcgcgc tcggctggat cttcagcgag gcgtacgatt cagagggtat
    16321 cctcgtcttc gcgatcctgt tctccatcgt tctcaatatt ttcagctatt ggttctcgga
    16381 caagatcgcg ctcaagtcgg ccggggcgcg cgagatcacc aaggaggaaa atccgacgta
    16441 ttggaacgtc gtcgagaacc tcgctatcac ggcggggctt cccatgccgc gcctgtatgt
    16501 cataaacgat cccgtgccga acgctttcgc caccggccgc aacaaggagc acgccgcggt
    16561 cgccgccacg accggccttc tccaaatgat ggaccgcacc gagcttgagg gtgtcatcgc
    16621 gcacgagctc tcgcacatcg gcaaccgcga cattctcatc tcgaccgtcg tggtggtgct
    16681 tgcgggctcc atcgccatgc tctccaatat tttccttcgc tcgaatttca tgttcggcgg
    16741 caggcgcggc aacgaccgca atggcggagg agcgatcttt ctcgtcatcg gcctcgtggc
    16801 ggcgatcctc gcgccgatct cggccacgct catccagctt gcgatctccc gcaagcgcga
    16861 atatctcgcc gatgcctccg gcgcgctcct cacgcgctat ccggaaggcc tcgcaagcgc
    16921 gctcgagaag atcgagcact atagcggcca gctgaagcat gccaataacg ccaccgcaca
    16981 cctctacatt tccaatcctt tcggcgaccg cgcgcatatc caaagcttca tgacccgcat
    17041 tttcagcaca catcctccga tcgaggaccg catccgcatt ctgaggggtc aagagaagat
    17101 gctgtaggaa taaggtataa aagtttcctc ttctttaaat ttgtggtata gtttctccga
    17161 acagatcttt gaaaggggaa ataaatgcgc aacggcgaac cgaatatgta cgccgggatc
    17221 tgcacactgc ggaacggagc tgtgtaaacg caatggtcga gaatgcaggc attccttgtc
    17281 tttaattcag ttgcgatacc gattgcattc ggtagctcac agcaagagac agtaaagata
    17341 cttataagtg tgattggatt cttcgtccat gtcgggcttt cgattactgt atggcaagct
    17401 ataagatgga ttagatattt agacgatgcg ctcgcagatt ttgaacaatt ggatactgaa
    17461 gttaattcag ttgcggcagt gcgtgttgcc atcttcagca atgtaatttt tgaacaacgt
    17521 cgtggtggat ggcttagatc gcatgcatat ttggttccgt ttggaatagg tgcaatcagt
    17581 tggttcctac gaatcgcagc acatttttat ttctgagaaa ggagatgttt atgattattg
    17641 atagagaaga acagaacaag gcagtagaac ggatgcatgc cgaaactaaa cgatactctc
    17701 gtattgatga ccgcatcgag aagggtttgt atattttcct cattcttggg gtaatattag
    17761 tcatctacgg tctggtgacg cataaaatac caatcgtctt ttaattgaaa agtgttcttt
    17821 acatagcacc cgctgacgac gggtgctttt gttaatcgat attcgtcact tttaaatgcc
    17881 ggaatagttg acaaaattct ggttcaagag ttttattggt gcgggaggat tctcaaatat
    17941 gccgcaaagt tccattggac aaagctccgc ggaggcggac gacgttcagg ctctcgcaaa
    18001 tgcgcttgcc aaactgtcgg atcattactc caagtctgaa gatccgaaaa tcctatgtgc
    18061 cgtagataca atagatgtca gtatcgaaat gctgatgaga gatgaaaatc cggatggatt
    18121 ttcgggaatc gcacgagcca gagttggtgc tgcacgaatc atgaatgtat cgtaatcata
    18181 cgacggccgg gtcttccgag acccggccgc tttttcatcc ctcttcttat ctccatttct
    18241 cttctcattt tgttcccatt ttcctccttc gcttggcgtt cgcgtctatt cctatattct
    18301 gcagaatata gacatggttg agaatatgag aataattgca aaatactctt atattctata
    18361 attctgcaga attatagaat attgacgcgc ttgagagcgg gaggagggga agagaggaga
    18421 gggaaaaggg cggaagagga ctttgacaaa ttttgccagt gggcttaaat acatgcggag
    18481 agagactatg caaccagttc cattgtcccg gccgaatgcg tccacgacgc aattcgatcc
    18541 gtatgttttt cagatcgaaa aggaaatttc caagttgttt gtcagggccc gtcatattgc
    18601 gaatactcac gaggattcca acttgcggca attctgccgc gtcttcaaag ccgcgtggcg
    18661 tcgctacgaa agcaggaaga aggataaaag cactacttcg cagagcgtgc tcggcgaaac
    18721 tgcgcggctt cttcaagata cgatcaattg gtcccgaacg aatgcgcaag agcacaatga
    18781 tccggctctc gagcttgctt gcgggatgat gcaaaaagcg ctggatatat acaaacacag
    18841 cggcagggct ccagcccgca gcacctgaat tcaaaatcac caaaaaccaa gcggccgatc
    18901 cttccgaatc ggccgctttt ctacctcagc agtttcgggt atataatttt atcaggaggc
    18961 ttgtatgacc gaggagcaaa agctcattga agacaagatc gatgctttca tagctcacat
    19021 ccaaagcctg gatattccga ctgaactgaa aaatcagttg aggcgctatc ttcgggcatt
    19081 cgtttcggcg cacatggtct gcaaacagga ggagctcgag aagacgatgc gggcttttct
    19141 tgccaattcc gtaaagcccg cgataagaat gctggatgaa ttcgataaac tggaagtcca
    19201 ctcgcggccg atttaaccaa atcggccgcg gtttttctta tatagaaagt ttgctactgg
    19261 tcttcgcgca tcaggagatt gatctgaaaa cctatgcgaa atacatcctc aaagaaggta
    19321 cgaatgagga gaagcgagag ctgatgggat gcttcaggtc gaagct
//
LOCUS       DASZYS010000056        21481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_570990, whole genome shotgun sequence.
ACCESSION   DASZYS010000056 DASZYS010000000
VERSION     DASZYS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 21481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21481
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570990"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHF05_03315"
     CDS             <1..272
                     /locus_tag="VHF05_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79986.1"
                     /translation="LDQAGTAKTITENGTDYSVASSTGAGAGNRYEETVYALPGTNPC
                     VGVRYFVHYGVIENYPAGAVKEFDRQSLLSTFDSMRKTLILNPTP"
     gene            340..981
                     /locus_tag="VHF05_03320"
     CDS             340..981
                     /locus_tag="VHF05_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HVS79987.1"
                     /translation="MKRKLELAFGIFGIAAACFIAINLYVSGVGAKYIAASTENLPQV
                     QAAIIPGAAVTQKGTPGAVLEDRIAMALSLYKQGKVSKILVSGSNGTLDYNEVDPVRK
                     VLMSNQIPQEDIFLDHAGFDTYSTMYRAKAVFQVDSAIIVTQDFHLPRALYLARSMGI
                     EAYGIPADRGTYTARNYFRELFSRPKAFFDVLIHRVPKYLGDPIPITGSGVGT"
     gene            1033..1368
                     /locus_tag="VHF05_03325"
     CDS             1033..1368
                     /locus_tag="VHF05_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79988.1"
                     /translation="MAIEPSLTNKRIARIVAISIAVDLVEAWLDTVPVIGWAANVAVD
                     GAAWLIFYFLFRRAGVRFRSASRRAIFAAGFLLDLIPVINMFAWTADVLAVIASVKRE
                     EERETAAAE"
     gene            1610..2728
                     /locus_tag="VHF05_03330"
     CDS             1610..2728
                     /locus_tag="VHF05_03330"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HVS79989.1"
                     /translation="MESLEEFFLIKHVKNWYLRFERPISSLSLVGGFIFDIFALNRVD
                     EPLENFWVGVHLCVAALGILLLNLLENGRMKALLRDKARSVFHFWLVMLTQFGFGGLL
                     STFLVFYFRSATLAASWPFLVLLAAAFILNEALKYHYARLTFQISMLFLSTYSFAIYI
                     VPVIVHRVGDDVFLLSGAVTLAFIALFLAAMRLLTREHFRENRKLIALLIVGLTALVN
                     GLYFLNVIPPIPLSLKSGGIYHSVAHSIDGSYALGAEASSWLDYFLPSQSIHTTPGET
                     VYAWSAVFSPTSLNTTIVHVWQYYDDTAGKWVTESRVPLSVTGGRGGGYRTYSTRSSL
                     TPGKWRVNVENLSGQIIGRLSFTVEQGIVPPALHTVVE"
     gene            complement(2935..4041)
                     /locus_tag="VHF05_03335"
     CDS             complement(2935..4041)
                     /locus_tag="VHF05_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HVS79990.1"
                     /translation="MNKVTTINLNGRAYQLEESGFETLRAYLDEAASRLSGNPDKDEI
                     ISDLEQAIAEKCDRHLTPNKNVVLAVEIDRILKEMGPVEADGRNSSDRESKESGNGSR
                     AGAPKRLYRVAEGEIIGGVCNGIAAYFNVDVTIVRIIFIILVILTSGGWVLAYFLMMM
                     VIPVATTSEEKATAHGEQFTAQDIVDRFKEGIQNITGPEWKNRKREWKKQKHEWKNRW
                     HAEMYHRKMRFAENVDYASHTGARVLAPLLGILSALIFIIWIFALMSVATTGLIFGLA
                     FPAGWPLWVGIIILCVAYGILTAPLKWIRQSLYAHGVYGPSDFRLLMALDSVLWIGFA
                     VFFLWLAYANIPPLHALADSFSAMAQAFFANLKS"
     gene            complement(4173..4511)
                     /locus_tag="VHF05_03340"
     CDS             complement(4173..4511)
                     /locus_tag="VHF05_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVS79991.1"
                     /translation="MNTSNTALQEKFGPIRTGLLEFVVLKIVSAKKVYVADILQELKN
                     TEFATQEGTLYPLLSRLRREALVDYEWKESEAGPPRKYYRLTDKGTGQLGELGEYWKK
                     INQTINALGK"
     gene            4942..7566
                     /locus_tag="VHF05_03345"
     CDS             4942..7566
                     /locus_tag="VHF05_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS79992.1"
                     /translation="MQTDRLSRKALALVLMILLAGAVNYTPPIQAASGSNLIPNPSVE
                     TASTSPSSVPLSWYKGGYGVNTRTLTYPVDGADGSKALRVEITAYTSGDAKWFFEPVP
                     VAGGETYTLSDKYRSNVPSTVDLQYRLADGTYKYPDVAVDLPASADWTDWSTTFTVPT
                     NYPSPVVNVSVFHLIQGVGWLETDAYSMADNSGGSGTTTPPEGTSTPDTEAPFVSVAS
                     VPNQPVSGTISLSATSSDNVGVAGVQFFLDNTAFGAEDSTSPYAVSWNTASSTDGAHS
                     VFAVARDAAGNSATSSAIAINVGNSTGTSTPPTGTSTPPTSNLILNGSMETASTSPSS
                     VPLGWYKGGYGSNTRTLTYPVAGIDGAKALRTEVTAYTSGDAKWFFEPVPVSGGQHYT
                     LSDMYRSNIPSTVDLQYQLADGTYKYTDLAIDLPASSDWTGFSKTFTVPTNYASPVKN
                     VTAFHLIQGVGWVDTDAFDLEAGSSSGTTTPPTGTTTPPTGTTTPPVDDGLLKNGSLE
                     QADPHNSSLPAGWTFTHAAGTAATAAYPVTSYDGSKAAEITVTNVGNGFGADWYSDAI
                     PVEGGDEYRFTDHFKSTAESWVEIEYQMSDGSYQYLDLLRLAPTAGAWKEGSNTFMVP
                     QGAESMTIYHLLQKNGTLDTDNYSLEKLPSGAFDQGMITLEFDDSLASQYENALPILR
                     DGGVTAGFYVITRALNESGYMTTAQVKDLQAQGFEVGAHTRNHPYLTTVSPTRLHNEV
                     AGSKADLENMGIEVNDFVYPYGDYNDIVEQEVENDGFAGARSVRSGFNTKNTDPYALM
                     DEHIENTTTIEDVKDWIDEAEAAHTWLIFEIHEVVPDTTGDQYATTPELLQEIVDYIK
                     QSGIKVVSTKEGLQMMKS"
     gene            complement(7609..8058)
                     /locus_tag="VHF05_03350"
     CDS             complement(7609..8058)
                     /locus_tag="VHF05_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79993.1"
                     /translation="MDELEDNRPAELHRHVHTRLKLRLVFYFLISIVLVMLVALHIIV
                     DQVNFLFPAIAIVIGLGVGIILSRTSHITWDHGAQKVISRFDILGGIILALYGVFEIF
                     KEEIVEEFVHGPAVVATSLALLTGIMIGRVIGIGGKIVRVLDEQNIG"
     gene            8138..8356
                     /locus_tag="VHF05_03355"
     CDS             8138..8356
                     /locus_tag="VHF05_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79994.1"
                     /translation="MKIEWNRVNWYSMSAAVVLFLAVLATGFYMGRIYEQLKTASMLQ
                     SIVDGEQNMMYVSHAPEDASTTTYIPVR"
     gene            8443..9009
                     /gene="cas2"
                     /locus_tag="VHF05_03360"
     CDS             8443..9009
                     /gene="cas2"
                     /locus_tag="VHF05_03360"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HVS79995.1"
                     /translation="MGIQEQQSRKRTKRKNLQFYILRTVAAAGVIAVGAVAPNVLGAL
                     GKLGFILPKRQGEFIKSAQRKLVENGYLVLSQGRLSLTKKGERALLLKEGFAKAKPRK
                     WDGKWRILVFDIPERRKSLREKVRNTLLDLGFYRLQDSVWVYPYECEDFITLLKTDFR
                     ISKELLYVIGDSIENDSHLIHHFSLKRK"
     gene            complement(9118..9825)
                     /gene="rsmG"
                     /locus_tag="VHF05_03365"
     CDS             complement(9118..9825)
                     /gene="rsmG"
                     /locus_tag="VHF05_03365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HVS79996.1"
                     /translation="MDELPNIAAEMGFRLSADQLESFSTYLRELQEWNARTNLTAVDD
                     ERGIALKHFADSLSLLPELPEGAFRLIDIGAGAGFPGLPLKIVRPEIDVTLLDATAKK
                     TEFLTHMIATLKLAGAAAIHGRAEEIARDEAYRETFDVAVARAVAKLPALAELALPFV
                     KVGGRFIAQKQAHDGGENEIEGARNALSELGGEILAVKNAPAPLPAGRQLVIISKISA
                     TPEKYPRRSGLPGKRPL"
     gene            10041..10784
                     /locus_tag="VHF05_03370"
     CDS             10041..10784
                     /locus_tag="VHF05_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79997.1"
                     /translation="MKKLIIALIVIAAVVGGVAYYRHATGGNNVVDMQATVSGTVKYV
                     APQAGTVTIETTDNSETSIAVTSSTTISDETGAAISLNDLQAGMSIQATGVRTPTSIV
                     ADTVRIVSRPPQGTDIAAPCPGVVTEDDNSLSCSMPITSRSTVSLPSADYSKNDLRID
                     PAASMGETFGASTGPDRWVRTFEATQPGAVTITVPAGNSSATAFKVSINVYGEPISNQ
                     PDNSAASDSNESGASGGSIDTQSGVQLEQ"
     gene            10998..12476
                     /locus_tag="VHF05_03375"
     CDS             10998..12476
                     /locus_tag="VHF05_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS79998.1"
                     /translation="MKKALLVAALFLASAGSLYAADDATAAAPEHHHGAPQTVYVQMT
                     DHGFEPAEVTINQGDLVVFQNADTASHWPASDIHPTHQIYPEFDPKRPIPGGESWSFT
                     FNRAGSWGMHDHLYPQFTGTIHVNAVLGFVDPTATSTQEFPTGFFHNLKIDALRIYYK
                     IFPSKLDKALSQVEFMKIAAEPKEIAYWLELLGPQPVMTKILAESGNGNTVDCHTQAH
                     QVGRVAYKIYGASVFKDGDDSCHSGFYHGAMEALIAEKGTDDFTKTVSDLCSNFDTRF
                     GNFECLHGVGHGVLAYTDYDLPKALTICQELPTEFEARSCYGGVYMENVVTGMGVGAK
                     PDHTTSWLNTDPQYPCDALPDDFNMVDDCYLMQTSWMLYVSNYDYPKVIGDCMAAPAD
                     HRHTCFKSFGRDVAGNTLRDPQKIVGYCGTVPQDDNNYESDCIAGAVNVIIDFWGEKY
                     TDEADPVCNLLDGAERLACQNLIAQRKRDDLSTQTSPSALMN"
     gene            12527..13630
                     /locus_tag="VHF05_03380"
     CDS             12527..13630
                     /locus_tag="VHF05_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVS79999.1"
                     /translation="MATSDRNNEETTWHFYRWSKDTWEAMISALESAAISIDFEQYYF
                     VDLASGRIGGRFLDVLEKKARQGVRVRLLLDATGSWEFYVSTTANRLRAAGAQVAFHE
                     TLPLRDASRFFHSFLRDHRRLLVIDGAAAFVGGTAIKEAARMWRDTHVELRGGIVRAL
                     ACEFDEIWKSVAEGLRHRQSALVARAGSWSVLPNGIRPSHRFIARFFLEGIRHAQKSI
                     YVSTPYFAPNHQLFRALRLAALRGIDVRLLLPQRSDSRFAERAAESYYSGALRAGIRI
                     FLYEPSFLHSKTIVIDDDWASVGSSNFDYLSMSFNYELNVLSYSSRFALEIKNHFFED
                     LQSAAEVMPEEWNGRFFLRKIFEKALIPLRIFV"
     gene            13716..14441
                     /locus_tag="VHF05_03385"
     CDS             13716..14441
                     /locus_tag="VHF05_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVS80000.1"
                     /translation="MDQETAHSSEHRRRGSSTKMILIIVAIVIIVVGGAFFAFKKGML
                     SLGEQKGKTVATVNGEPIYQHDVDLRLNQLKPTYAAQGVNLDDQATLTTVEKQIVDEI
                     VNEDLILQDAKTKNISVTDAEVKDAMDQLVASAGSQDALDTQIKQFGMSSADLQDTLQ
                     RNLILQKYVDQLSAAQNITVSEKEISDAYDQMTQGQAASTTPALKDVHDQVEEQVKNQ
                     KIAAQVLAVVNQLKQTAKINILI"
     gene            14457..15014
                     /locus_tag="VHF05_03390"
     CDS             14457..15014
                     /locus_tag="VHF05_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVS80001.1"
                     /translation="MLTALAGYKYLLVFGGLILSGDIFLVSFIYSAKFGFVTLPYLFL
                     TALLASAVSDFMWYALGRFIGLQRIQKIWFLRKNPALVEKLSAMFEKNGAKLLFMCKF
                     LYGTRVVTEVITGMHRMKYAKYFLINLCTTIIWTLFLFLFVTAVDASVSSIKSLLAKI
                     EIVITVFAAFFVLAHFAAKRVLWKK"
     gene            15071..15577
                     /locus_tag="VHF05_03395"
     CDS             15071..15577
                     /locus_tag="VHF05_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80002.1"
                     /translation="MKIPEHIRSRLGDSPAFLLVTSKQEAALYAINGEDLAELGSFVV
                     ERPADTDKEGEFKSRSRGGSASGTAYERTDDAQIRDFTSLMEVMARSKLYGASGRLYA
                     FAPAAVKSHLMKALGGSARSLEVIFTEGNYMHSSPVDLLDKIAREFESKRVVPTDPAA
                     QRILDIPI"
     gene            16004..17269
                     /locus_tag="VHF05_03400"
     CDS             16004..17269
                     /locus_tag="VHF05_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80003.1"
                     /translation="MGDLTRKIVAAASIASTIAFYSAGVAAAKTNDDIQSVRVVSSNS
                     ASVTNSASVNASTGGNVANGGNAGMGGAGGNGGTTGDAAIMGLHVANGGNAGNGGAGG
                     ASGDAGTGGDSGAASSVGTAGRASNGGDSGTADALSTSGDAGNGGDSGMGGAGGEGGN
                     GGAGGTTGSQTLDVTGTARGGNGGEGGNGGAGGVIVTGDAGADITMNNDVNSSDTRVN
                     DPAQSTEDLNTSNSIDRNHSITVRSENGSVLDNIFTAMLRTGNNTANAGNGNDGGAAG
                     NGGSTGGAYIGTAAFASGGNAGTGGDGALSGMGGNGGTSGIADSASNGGNANQGGTSG
                     DAISTSLTGQGGNGGASGDAGAGGEGGNGAQGGTTGDQSLTASSDVNGGNGGEGGNGG
                     EGGSILTGASVARTSFINVINRAITRVTR"
     gene            17381..17959
                     /locus_tag="VHF05_03405"
     CDS             17381..17959
                     /locus_tag="VHF05_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80004.1"
                     /translation="MNEHVILQTRSVIPAVALFTVLFGAGLYAGNLFAATTSGNVSSR
                     ATVYAFSSNGADGADGQDGAPGRDGISGQNGESSASVKIVTTVNGQVVQNIDEHRASD
                     DGSPVVIDISTTTSYDSGNGHAGDSGDAPPAAREGESVDVSASADAIASDETAAATSE
                     TATSSDEVSGFVGFYSFIRSIFSHVASFFHLA"
     gene            17997..20546
                     /locus_tag="VHF05_03410"
     CDS             17997..20546
                     /locus_tag="VHF05_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80005.1"
                     /translation="MILFTPYSVSFSVRVTNADEEASSSSSTSDISETAGASSADTND
                     ATAAPETAISSGASTVSSEAGNNLNTNSVSVGDATDTPPDDNAGGDETATSSGESATT
                     SDATSTVSDNSDNAATTSDATTSLSVVNDSTADASSTMDLDSSTGANAASSTGDVMVQ
                     SGDAIANASLLNVVNTNLVNSYGELLALSSLLMNSVNQFDLRSLDFFASSSIRCAICG
                     GTTLGSLSVLNNGTASISNALSAHATSGGNAADSASGTADILGGDAYAAGNVINFANN
                     NLVDSKYLFMTYNNFGDWGGDIVFPNAETLMKILLSGASISPSNLSLSNTGFADVDNN
                     VSTTATSGNNSFTGGTSTVSSGSSDATTNILNLLNSNLVGGASMNVLLNIEGSWSGSI
                     IGLPPGVEWKKTDHGIALSFDSIEGSDDATDSPDTDVSNGATTGIGNSIDVSAASGDN
                     HASGADGSVESGDAYAAANVINVANTNMIGRNMFWAIINIFGDWSGDVSFGKPDLWIG
                     GHADITGPSVSPGGEVTYHFTVINNGDLPATGVSVHTDLGSDYLKDEGTDASTILTDL
                     GTIAPKESKSFDITARLDRSMAGKETAITSAKVTSIEHDNNLDDNTEDISVLLGSPRH
                     HGSSRPKTEEATSTASTTPLLDVSASFDGPSIVPASSTVAYHVVIKNDGDAPVYNAEF
                     HERLLDGTGASLHGEVWDLGTIAPHDELNIDYSVFYDPDTPSGVYTASGFVQGTAGED
                     AASAPVASNVSAVSLMVRGLHLVPAPGKAPLVTKPTISTQGTSSSSTAETAASAAPSD
                     EPQNPIPPANDRGLLASVFFSAPSAGELTGFSLALLAAAVGIMLYRRRQYA"
     gene            20626..21282
                     /locus_tag="VHF05_03415"
     CDS             20626..21282
                     /locus_tag="VHF05_03415"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HVS80006.1"
                     /translation="MNKKALVAIIAVFAAFGGLLLWGLGITPSEHILANISSFEDCVS
                     AGLDSTGTPPSCATPDGRTFAKLGAPPAPPQEATSTDIATDTAPTIAVDAPQPDDAVV
                     SPLRVSGKARGWYFEATFPVRLLDSKGRTVAVGAAVASGDWTSRTEVPFTARLTFSAP
                     STETGTLVLERSNSSGLPDQSAEVSIPVRFSTVAGEATSTATTSSGEGAATGSPAETR
                     "
BASE COUNT         4647 a         6250 c         6078 g         4506 t
ORIGIN      
        1 tccttgatca ggcgggaacg gccaagacga ttaccgaaaa cggcaccgat tactcggtcg
       61 cctcgtcgac cggcgcgggc gcgggcaacc gctacgaaga gacggtctac gcccttccgg
      121 gaacgaatcc ctgcgtcggc gtgcgctact tcgtccacta cggcgtgatc gagaattatc
      181 ccgcgggcgc ggtgaaggag tttgaccgcc agtcgctcct cagcactttc gactcgatgc
      241 ggaagactct aatcctcaat ccgaccccct aacatctctc gcgaaaaccc gcctgcgaat
      301 gaacgcaggc gggtttttca tttgtgatag tatgggtgca tgaagcgcaa actggagctt
      361 gctttcggga ttttcggcat tgccgcggcc tgctttatcg ccatcaatct ctacgtttcg
      421 ggcgtcggcg cgaagtacat cgccgcctcg acggagaacc tgccgcaggt tcaggcggcc
      481 atcatccccg gcgcggcagt tacccagaag ggaactcccg gcgcggtgct cgaggaccgg
      541 atcgccatgg cgttgagcct ctacaagcag gggaaggttt cgaagattct cgtatcggga
      601 agcaatggca cgctcgacta caacgaagtc gatccggtgc gcaaggtgct catgtcgaat
      661 cagattccgc aggaggacat ctttctcgat catgcgggct tcgacaccta cagcacgatg
      721 taccgcgcga aggcggtctt ccaggtcgat tcggcgatca ttgtgacgca ggatttccac
      781 ctgccgcgcg cgctctacct cgcaagaagc atggggatcg aggcgtacgg cattcccgcc
      841 gatcggggaa cgtacaccgc caggaattat ttccgcgagc tcttctcgcg gccgaaggcg
      901 tttttcgacg tgctgattca tcgcgtgccc aagtatttgg gagacccgat tcccattacc
      961 ggaagcggcg tcggcacctg attcggattc gccgtaatcg gctcttcatg ccgcgcgtgc
     1021 attcttactt ttatggctat cgaaccttct cttaccaata agcgcatcgc cagaatcgtc
     1081 gcgatatcga tcgccgtcga tctcgtcgag gcatggctcg ataccgtgcc tgtcatcggc
     1141 tgggcggcga acgtcgcggt cgatggagcg gcgtggctca tattttattt tctgttcagg
     1201 cgggcgggag tgcgattccg ctcggcttcg cgccgcgcca tcttcgcggc cggattcctc
     1261 ctcgacctta ttccggtcat caacatgttc gcctggaccg ccgacgtcct tgccgtcatc
     1321 gcgagcgtga agcgcgaaga ggaaagggaa acggcggccg cggagtagga gcgtattctt
     1381 cagcgggctg tataatatag aagcagtctc agtaaaaaac gcttactcta taaacaaact
     1441 aatgtgacgc ttggctgccg aaagcccgaa atttcggctg caaaatcgga aaacctcgac
     1501 ttatcgtagc ttaggctacg ccgcgctcgg tttttacgat ttttcgcttc gaaatttcag
     1561 tctttcgcca agccattttt actgagactg cttctaagca cgcatgtttt tggaaagttt
     1621 ggaggaattc ttcctcatca agcacgttaa gaactggtat ctccgtttcg agcggccgat
     1681 ttcgtcgctt tcgctcgtcg gcggcttcat cttcgacatc ttcgccttaa accgcgtcga
     1741 cgagccgctc gagaatttct gggtcggcgt gcatctctgc gtcgcagcgc tcggcatcct
     1801 tctcttgaac ctcctcgaga acggccggat gaaggcgctt ctgcgcgaca aggcgaggag
     1861 cgtcttccac ttctggctcg tcatgctgac gcagttcggc ttcggaggcc tcctctccac
     1921 cttcctcgtt ttttatttcc gcagcgcgac gctcgccgcg agctggccgt tcctcgtcct
     1981 cctcgcggcg gcgttcatat tgaacgaagc gctcaagtac cattacgcgc ggctgacctt
     2041 ccagatcagc atgctcttcc tttcgaccta ttcctttgcg atctacatcg tgccggtcat
     2101 cgtccatcgc gtgggcgacg acgtattcct gctttccggc gccgtcacgc tcgcgttcat
     2161 cgcactcttt ctcgccgcca tgcggcttct cacccgcgag catttccggg aaaacaggaa
     2221 gcttatcgcg cttctcatcg tcggactcac cgcgctcgtc aacgggctgt atttcctgaa
     2281 tgtcattccg cccatcccgc tctcgctcaa atccggcggc atctatcact ccgttgcgca
     2341 tagcatcgac ggcagctacg cgctcggcgc cgaagcttcg agctggctcg attacttcct
     2401 tccgtcccaa tcgatccaca ctacgcccgg cgaaaccgta tatgcctgga gcgcagtctt
     2461 ctcgccgacc agtctcaata cgacgatcgt ccatgtctgg caatattacg acgatacggc
     2521 gggcaagtgg gtgacggaga gcagggtgcc gctttccgtg acgggcggca ggggcggagg
     2581 ctatcggacg tactcgacgc gaagctcgct cacccccggc aagtggcgcg tcaacgtgga
     2641 aaatctctcg gggcagatca tcggccgcct ctcgttcacc gtggagcagg gaatcgtccc
     2701 gccggcgctt cacacggtgg tggaatagga agaaggttga gagctgtgag ctatcagcgg
     2761 ttggccaacc ttgtggtttg acctcaatag attatgcagg gtagacctgc agaatctaca
     2821 tacgttataa aattgctata gcaattttat aacgtatgtg aattatttct aatatttctg
     2881 atatgtataa aaaaccttgc gatatatcgc aaggtttttt tattcacgct gtgattacga
     2941 cttgaggttc gcgaagaacg cctgcgccat cgcgctgaag ctgtcggcaa gcgcatggag
     3001 cggcggaata ttcgcgtatg cgagccagag gaagaaaacg gcgaagccga tccagagcac
     3061 cgagtcgagc gccatgagaa gccggaaatc ggacgggccg tagacgccgt gtgcataaag
     3121 cgattggcgg atccatttca agggagcggt aaggatgccg taggcgacgc agaggatgat
     3181 gattccgacc cagagcggcc atccggcagg gaaggcaagg ccgaagatga ggccggtggt
     3241 ggcgacggac atgagcgcga agatccagat aatgaagatg agagcgctca ggattccgag
     3301 gagaggagcg agcacgcgcg ctccggtatg cgacgcgtag tcgacatttt cagcgaagcg
     3361 cattttccgg tgatacattt ccgcgtgcca gcgatttttc cattcgtgtt tctgcttctt
     3421 ccactcgcgc ttccggtttt tccattccgg accggtgatg ttttgaatcc cttccttgaa
     3481 acggtcgacg atgtcctggg cggtgaattg ctctccatga gcggtcgctt tctcttcgga
     3541 ggtcgtcgca acgggaatca ccatcatcat gaggaaatag gcgagcaccc atccgccgct
     3601 cgtcaggatt acgagtatga tgaagatgat ccgcacgatc gtcacatcga cgttgaagta
     3661 cgctgcgatt ccgttgcata cgccgccgat gatctcgcct tcggcgaccc tgtagaggcg
     3721 cttcggcgct ccggcgcggc ttccatttcc gctttctttg ctttcccgat ccgaagaatt
     3781 ccttccgtcg gcttcgaccg ggcccatttc cttgagtatc cggtcgatct cgaccgcgag
     3841 caccacgttt ttgttcgggg tgagatgcct gtcgcacttc tcggcgatcg cctgttcgag
     3901 atcggatatg atctcatcct tgtccggatt gccggaaagg cgcgaggcgg cttcgtcgag
     3961 ataggcgcgc agcgtctcga agcccgattc ctcgagctgg taggcgcggc cgttcaaatt
     4021 gattgttgta accttgttca tcccgttaga gataggaaat ggctgccatt tccgtaccat
     4081 ataaattaat ttgcgttgat aattttttca ttcccgtatt cgatcctagc gatatttctt
     4141 aatcattcga tctctaacgg gattcatgat aattattttc ctaatgcgtt gatggtttga
     4201 ttgatttttt tccaatattc cccgagttcg ccgagctggc ccgttccctt gtccgtcagc
     4261 cggtaatatt tccgcggcgg cccggcttcc gattccttcc attcgtaatc gacgagggct
     4321 tcgcggcgga ggcgcgagag caggggatag agcgtgcctt cctgggtggc gaattcggta
     4381 ttcttgagct cctgcaggat atccgccacg tagacttttt tcgcagaaac gatcttcaaa
     4441 acgacgaatt ccaaaagccc cgtgcggatc gggccgaatt tctcctgtag ggctgtattt
     4501 gatgtattca tcccgttaga ggcagatcac gctcaccgcg ttttccgcat aattaataga
     4561 attcttttta attaatgttt tatccattgt ggataagata acgtgatcac ggtctctaac
     4621 gggattcata ttaaagctac cttgttactt aaaggtagca aataccaaaa aggaagtcaa
     4681 ccctgtagta gattttggca tcgggccata ggtccgcgga tgcacaaagc atccgcaatg
     4741 ccaaaattca ctacagggca aacggcccga agaaatggga gggaaggagg tcccgacgca
     4801 attgcgagat tcgccaaagc tcgaccattc atcgtcgctt catatcccat gtgcgacaat
     4861 cacggcagta acgtcaaatc attatcaatc tcaaggagat tcgccgctcg gccgatctcc
     4921 caaacctcta actcacttca catgcaaaca gatcgattat caaggaaggc gcttgcgctc
     4981 gtcctcatga ttctcctggc gggcgcggtc aattacacgc ctcccatcca ggcggcttcc
     5041 ggaagcaatc tgatcccgaa cccttcagtc gagacggcct ccacgtctcc ttcatccgtg
     5101 ccgcttagct ggtacaaagg cggctatggc gtaaacaccc gcacgcttac gtatccggtg
     5161 gacggcgcgg acgggtccaa agcgctccgc gtcgagatta ccgcctacac ctccggcgac
     5221 gccaaatggt tcttcgagcc cgttcccgtg gcgggaggag aaacctacac gctctcggac
     5281 aagtaccgat cgaacgttcc ttcaaccgtc gatcttcagt accggcttgc cgacggcaca
     5341 tacaaatatc ccgacgtagc tgtcgatctg ccggcgtcgg ccgattggac cgattggagc
     5401 acgacattta ccgtaccgac aaactatccg tcgccggtcg tgaacgtgtc ggttttccat
     5461 ctcattcagg gcgtcggctg gcttgagacc gatgcgtata gcatggcaga taattcaggc
     5521 ggaagcggca ccaccacgcc gccggaaggc acttccactc ccgatacgga agcgccgttc
     5581 gtgtcggtgg cgtccgttcc gaatcagccc gtttccggca cgatcagcct ctcggccacc
     5641 tcctccgata atgtcggcgt cgccggagtg caatttttcc tcgacaatac ggcgttcggc
     5701 gccgaagatt cgacctctcc gtacgcggta agctggaaca ccgcatcctc gaccgacggc
     5761 gcacatagcg tctttgccgt cgcgcgcgac gcggcgggca attccgcaac gtcgagcgcg
     5821 attgcgataa atgtcggcaa ttcaacgggt acctcgacgc cgcccacggg tacgagtacg
     5881 cccccgacga gcaatctgat cctcaacggc tcgatggaaa ccgcttcgac atcgccgtca
     5941 tccgtgccgc tcggatggta caagggcggc tacggctcaa acacgagaac gctcacatat
     6001 ccggtcgcgg gcatcgacgg cgcgaaagcg cttcgcaccg aagttaccgc ctacacctcc
     6061 ggcgacgcca aatggttctt cgagccggtg ccggtaagcg ggggacagca ctacacgctt
     6121 tccgacatgt atcgctcgaa tattccgagc acggtcgacc ttcaatatca gctcgccgac
     6181 ggaacgtaca agtatactga cttggcgatt gatctgcccg catcttccga ttggaccgga
     6241 ttctcgaaaa cgttcaccgt tccgacgaac tacgcgtcgc cggtgaagaa tgttacggcc
     6301 tttcatctca tccagggcgt gggctgggtt gataccgatg ccttcgatct cgaagccggc
     6361 agttcaagcg gcacgacgac gcctccgacc ggcaccacca ctccgcccac cggcactacc
     6421 acgcctccgg tcgacgatgg gcttttgaag aacggctcgc tcgagcaagc tgatccgcac
     6481 aattcatcgc ttccggccgg ctggactttc acgcatgccg ccggaacggc cgctaccgcc
     6541 gcgtatccgg taacaagcta tgacggcagc aaggccgcgg agattacggt cacgaacgtc
     6601 gggaacggct tcggcgccga ttggtattcg gacgcgattc cggtcgaggg cggcgatgaa
     6661 taccggttca ccgaccattt caagtcgacg gccgagagct gggtcgagat cgagtaccag
     6721 atgtccgacg gctcctacca atatctcgac cttctgcggc tcgcgccgac cgccggcgcc
     6781 tggaaagagg gcagcaatac cttcatggtt ccgcagggag cggagagcat gacgatctac
     6841 caccttctcc agaaaaacgg cacgctcgat accgacaatt attcgcttga gaaactgccg
     6901 tcgggcgcat tcgaccaggg aatgatcacg ctcgagttcg acgacagcct ggcgagccag
     6961 tacgagaatg ccctgccgat cctcagagac ggcggcgtga ctgccgggtt ctacgttatc
     7021 acccgcgcgc tcaacgaatc gggctatatg acgaccgcgc aggtgaagga tcttcaggcc
     7081 caaggcttcg aggtcggcgc gcatacgagg aatcatccct atctcaccac cgtgtccccg
     7141 acgaggcttc acaacgaagt ggccggatcg aaagccgatc ttgaaaatat gggcatcgag
     7201 gtgaacgatt tcgtgtaccc gtacggcgac tacaacgata tcgtggagca ggaagtggag
     7261 aacgacggct ttgccggcgc ccgaagcgtc cgaagcggat tcaataccaa gaataccgat
     7321 ccgtacgcgc tcatggacga gcatatcgag aacacgacga cgatcgaaga cgtaaaggat
     7381 tggatcgacg aagccgaagc ggcccacact tggctcatct tcgagatcca tgaagtcgtc
     7441 cccgacacga cgggcgacca atatgccacg acgccggagc ttctccagga aatcgtcgac
     7501 tatatcaagc agtccggcat caaggtcgtg agcaccaagg aagggcttca gatgatgaaa
     7561 agctagaaaa aatcagaatg aaaaaaccgc cgtggaggcg gtttttttct agccgatatt
     7621 ctgctcgtcg aggacgcgga cgatcttgcc gccgatgccg atgacccggc cgatcatgat
     7681 gccggtaagg agggcgaggg atgtggcgac taccgccggc ccatgcacga attcctcgac
     7741 gatctcctcc ttgaaaatct caaagacgcc gtagagggcg aggatgatgc cgcccaggat
     7801 gtcgaaacgg gaaatgactt tctgcgcgcc gtgatcccat gtaatatgcg acgtgcgcga
     7861 aagaatgatg ccgacgccca ggccgatgac gattgcgatg gcaggaaaaa ggaaattaac
     7921 ctggtcgacg ataatgtgaa gcgccacgag catcaccagc acgatggaaa tgagaaagta
     7981 gaaaaccagc cggagcttga gcctggtatg cacgtggcgg tggagttctg cgggtcgatt
     8041 gtcttcgagc tcgtccatac gatctgctta tactaccact attcgtttcg gggcgcgatt
     8101 actgccgagc ttgcctcccg gtgctacaat aaggctcatg aaaatcgaat ggaacagggt
     8161 gaactggtat tcgatgagcg cggcggtcgt cctctttctc gcggtgcttg cgaccggctt
     8221 ctacatgggc cgcatctacg agcaattgaa gaccgcgtcg atgctccagt cgatcgtcga
     8281 cggcgagcag aacatgatgt acgtctcgca cgcgccggaa gacgcatcga cgacgacgta
     8341 tattccggtg cggtaggcgg cgctgtaaga taagctcatc catgtacaca tgcatatcat
     8401 tcatatacgt tataatattg ctatagcaaa tttataacgt atatgggaat tcaggagcag
     8461 cagagcagaa agaggacgaa gagaaagaac ctgcaatttt atattttacg taccgtggcg
     8521 gcggcaggtg ttattgcagt gggagccgtt gctcccaacg tgctgggtgc gctcggcaaa
     8581 ctgggattca tattgccgaa aaggcagggc gaatttatca aatcggctca gcgcaagctg
     8641 gtggaaaatg ggtaccttgt cttatctcaa ggacgcttgt cactgacaaa gaagggagaa
     8701 cgagcccttt tgctcaagga aggctttgca aaggcgaaac cacggaagtg ggatggaaaa
     8761 tggcgaattt tagtttttga tatccccgag agaagaaaat cattacggga aaaagtgagg
     8821 aatactctcc ttgatcttgg attttaccgt ctgcaggaca gtgtatgggt atatccatac
     8881 gagtgtgaag atttcattac gcttctcaaa accgattttc ggatcagtaa ggagcttctc
     8941 tatgtcattg gcgattcgat tgagaacgat tcgcatctta ttcatcactt ttccttgaaa
     9001 cgaaaataag aacagtggca caaatatctt tttactacgt tataaatttg ctatagcaaa
     9061 tttataacgt agtagttgga agtggaggct ggaaattaag aagtggaaat tggaaaatca
     9121 gagcggacgc tttccaggca ggccggagcg gcggggatat ttttccggcg tcgctgaaat
     9181 tttcgagatg atgacgagct ggcggccggc ggggagcggc gcgggcgcgt ttttcacggc
     9241 gaggatttcg ccgccgagct ccgagagggc gttgcgggcc ccttcgatct cattttcgcc
     9301 gccgtcatgc gcctgcttct gggcgatgaa gcggccgccg actttgacga acggaagggc
     9361 gagttcggca agcgcgggaa gcttcgcgac cgcccgcgcg accgccacgt cgaacgtttc
     9421 gcggtacgct tcgtcgcgcg cgatttcctc ggcccggcca tgaatggccg cggcgccagc
     9481 gagtttgagc gtcgcgatca tgtgcgtgag gaattccgtc ttcttcgcgg tcgcatcgag
     9541 aagcgtgacg tcgatttccg gacggacgat cttaagcggc aggccgggaa agccggcgcc
     9601 cgcgccgatg tcgatgagcc ggaacgcgcc ttcggggagc tccgggagaa gggagaggga
     9661 atcggcgaag tgcttgaggg cgatgccgcg ttcgtcgtcg accgcggtaa ggttggtacg
     9721 ggcattccat tcctgaagct cccgaagata ggtcgaaaag ctctcaagct gatccgccga
     9781 gaggcggaag cccatctctg ccgcgatatt tggaagctca tccatgatgc gccgaattct
     9841 accacattcc ggctatttcg cggccctatg caatgctttt cactcttcct taacaaatgg
     9901 aggtataatg ggtggtgagg atcatttccg gccggagaga ggcggattgc cggagacgcc
     9961 tttgggaata tgcttggaat ttagccattt ttttatggac agcctgccgg aaaagcggct
    10021 cgcccgcatc tttcactaga atgaagaaat tgattatcgc gcttatcgtg atcgccgccg
    10081 tcgtcggagg cgtcgcctat taccgccacg ccacgggcgg aaacaacgtc gtggatatgc
    10141 aggcgaccgt gagcggcacc gtgaagtatg tcgctccgca ggccggaacg gtcacgatcg
    10201 aaacgacgga taattccgag acgagcatcg ccgtgacatc ttcgacgacg atctccgacg
    10261 agaccggcgc tgcgatctcc ctgaacgacc tgcaggccgg catgtcgatc caggcgacgg
    10321 gcgtccgcac gccgacctcc atcgtggccg ataccgtgag gatcgtgtcg cgtccgccgc
    10381 agggcacgga catcgccgcg ccgtgccccg gagtggtaac cgaggacgac aacagtctct
    10441 cctgcagcat gccgatcacg tcgcgcagta ccgtctccct tccgtccgcc gattattcca
    10501 agaacgatct caggatcgat ccggcggcca gcatgggcga aactttcggc gcgtcgaccg
    10561 gtccggaccg gtgggtgagg accttcgagg cgacacagcc gggcgccgtt accatcaccg
    10621 ttcccgccgg caattcgagc gcgaccgctt tcaaggtgag catcaacgtg tacggcgaac
    10681 cgatatcgaa tcagccggat aattcggcgg cttccgattc gaacgaatcg ggggcaagcg
    10741 gcggctcgat cgatacgcaa tcaggcgtgc aactcgaaca ataatgcatt ctgcaaaaca
    10801 agccgcatgg cttgtttttt tgatatcggg gaaacgagca tttcgccgga aggcgaggtg
    10861 cgatatgatt aggccatgaa tcccgtcaga ggcggctttg cacgcagagg gaattcattt
    10921 acggaaaaac atgagcatgg ttcgattgaa tttagaaatt aaacacggag agcaaagcct
    10981 ctcctgcctc taacgggatg aagaaagctt tgcttgttgc ggcgctcttt ctcgcttccg
    11041 ccggaagcct gtatgccgcg gacgacgcga cggctgccgc gcccgaacat caccacggcg
    11101 ctccgcagac cgtctacgtc cagatgaccg atcacggctt tgaaccggcc gaagttacga
    11161 tcaatcaggg cgacctcgtc gtcttccaga atgccgatac ggcaagtcat tggccggcat
    11221 cggatatcca tccgactcat cagatctatc ccgagttcga tcccaagcgg cccattccgg
    11281 gaggggagtc gtggtcgttc accttcaatc gcgccggctc gtggggcatg cacgatcatc
    11341 tttatccgca gttcacgggt accattcacg tgaatgccgt tctaggcttc gtcgatccga
    11401 cggcgacttc cacgcaagaa tttccgaccg gttttttcca taatctcaaa atcgatgcgc
    11461 tccgcatcta ttacaagatc tttccttcga agctcgacaa agcgctctca caggtcgaat
    11521 tcatgaaaat cgcggccgaa ccgaaggaga tcgcgtactg gctggaactc ttaggccccc
    11581 agccggtcat gacaaaaatt ctcgccgaat cggggaacgg caacaccgtc gattgccaca
    11641 cgcaggccca ccaggtcggg cgcgtcgcct acaagattta cggggcatcc gtcttcaagg
    11701 atggcgacga ttcctgccat tccggctttt accacggcgc catggaagcc ctcatcgccg
    11761 agaaggggac cgatgatttc acgaagacgg tatccgacct gtgttcgaat ttcgataccc
    11821 gcttcggcaa cttcgaatgc ctccacggcg tcggccacgg cgtgctcgcg tataccgatt
    11881 acgacctgcc gaaggcgctt accatctgcc aggaactgcc gaccgaattc gaggcgcgct
    11941 catgctacgg cggcgtctac atggaaaacg tggtgaccgg catgggcgtg ggcgcgaagc
    12001 ccgaccatac cacttcctgg ctcaataccg atccgcaata tccatgcgac gccctgcctg
    12061 atgatttcaa tatggtggac gactgctacc tcatgcagac aagctggatg ctgtacgttt
    12121 ccaattacga ttatccgaag gtcatcggcg actgcatggc cgctcccgcc gatcatcggc
    12181 acacctgctt caagagcttc gggcgcgacg tggcgggcaa cactctccgc gatccgcaga
    12241 agatcgtagg atactgcggc accgtcccgc aggacgacaa caattacgaa tccgactgca
    12301 tcgccggcgc ggtgaacgtc atcatcgact tctggggaga gaaatatacc gacgaagccg
    12361 atcctgtctg caatctgctc gacggcgcgg agcgcctcgc gtgccagaat ctcatcgcgc
    12421 agcgcaaacg cgacgatctt tccacgcaaa cgtcgccttc ggcgctcatg aactagaaag
    12481 ctcagccgtt aatcttttat tcatccggat cgctaagatt aaaagaatgg caacgtccga
    12541 ccggaacaat gaagaaacga cgtggcattt ctaccgctgg tcgaaagata cgtgggaagc
    12601 gatgatttcc gctctcgaat cggcggccat atcgatcgac ttcgagcagt attatttcgt
    12661 cgatctcgct tccggccgca tcggcggccg tttccttgac gtccttgaga aaaaagcgcg
    12721 ccagggcgtc cgagtgcggc tgcttctcga cgcgacgggc agctgggaat tttacgtctc
    12781 gacgactgca aaccggctgc gagccgcggg cgcgcaggtc gcattccacg aaacgctgcc
    12841 acttcgcgat gcgagccgct ttttccattc gtttctccgc gatcaccggc gcctcctcgt
    12901 gatcgacgga gcggccgcct tcgtcggcgg gacggcgatc aaagaagcgg cgagaatgtg
    12961 gcgcgacacg cacgtcgaac tccgcggcgg cattgtccgc gcgcttgcct gcgagttcga
    13021 tgagatttgg aaaagcgtcg cggagggatt gcggcaccgc cagtccgccc tggtcgcccg
    13081 ggcgggctcg tggagcgtcc tcccgaacgg catccgcccc tcgcatcgct tcatcgcacg
    13141 cttctttctc gagggcatca ggcacgcgca gaagagcatt tacgtttcca ctccgtattt
    13201 cgcgcccaac catcaattgt tccgcgccct ccgcctcgcg gccctccgcg gcatcgacgt
    13261 gcggcttctt cttccgcagc ggagcgacag ccgcttcgcg gagcgcgccg cagaatcgta
    13321 ttactcgggc gccttgcgcg cgggcatccg gatcttcctg tatgagccgt cgttcctcca
    13381 ttcgaagacg atcgtcatcg acgacgactg ggcttccgtc ggcagctcga atttcgatta
    13441 cctttcgatg agcttcaatt acgaattgaa cgtcctttcc tacagttcgc gcttcgccct
    13501 ggaaataaag aaccatttct tcgaggacct ccagagcgcg gccgaagtaa tgcccgagga
    13561 gtggaacggc cgttttttcc tgcgcaagat atttgaaaaa gcgctgatac cgctgcgcat
    13621 tttcgtgtag cgccccctga aatcccgttg gggaaaagct gattgcgcct tcatatttat
    13681 ggtatacttt acccataatc gctttgtagt catatatgga tcaagaaaca gcacactcgt
    13741 ccgagcatcg caggcgcgga tcatccacga agatgattct cattatcgtg gcaatcgtca
    13801 ttatcgtcgt cggcggagcg ttcttcgctt tcaagaaggg catgctctcg ctcggggagc
    13861 agaagggcaa gacggtcgct acggtgaacg gcgagcctat ttatcagcat gatgtcgact
    13921 tgaggctgaa ccagctgaag cccacgtatg cggcgcaggg agtcaatctc gatgaccagg
    13981 cgacccttac gaccgtcgaa aagcagatcg tcgacgagat cgtgaacgag gacctcatcc
    14041 tccaggatgc caagaccaag aacatcagcg ttaccgacgc ggaagtcaag gacgcgatgg
    14101 atcagctcgt ggcatcggcc ggcagccagg acgccctcga cacgcagatc aagcagttcg
    14161 gtatgagctc ggctgatctc caggatacgc tccagaggaa cctcattctc caaaaatacg
    14221 tcgatcagct ctctgcggcc cagaatatca ctgtttccga aaaagagatt tccgacgcgt
    14281 atgatcagat gacccagggc caggcggcat cgaccacgcc cgcgctcaag gatgtccacg
    14341 accaagtgga agagcaggtc aagaatcaga agatcgcggc gcaggtcctt gccgtcgtca
    14401 atcagctcaa gcagaccgcg aagatcaaca ttctcatcta atttaaccgc tggcctatgc
    14461 ttacggcgct cgcggggtac aaataccttc tcgtattcgg cggcctgatt ctctccggcg
    14521 acatctttct cgtcagtttc atctattcgg cgaagttcgg tttcgtaacg cttccgtact
    14581 tatttctcac ggcgctcctt gcgagcgccg tgagtgattt tatgtggtat gccctcggcc
    14641 gcttcatcgg ccttcaaagg atccagaaga tatggttcct gcgcaagaac cccgcacttg
    14701 tggagaagct ttcggcaatg ttcgagaaga acggggcgaa gctccttttc atgtgcaagt
    14761 tcctctacgg cacgcgcgtg gtaaccgaag tcatcacggg catgcaccgc atgaaatacg
    14821 cgaagtactt cctcattaat ctctgcacca cgatcatctg gacgctcttc ctctttctgt
    14881 tcgtcactgc ggtcgatgcg agcgtgagct cgatcaagag ccttctcgcg aaaatcgaga
    14941 tcgtcatcac ggttttcgcc gcgtttttcg tcctggccca tttcgccgcc aaaagagttc
    15001 tctggaagaa gtagggaaac agcccttgcc gcttcatttt ttcttaaaag gaaggaggta
    15061 ggattgcgct atgaaaatac cagaacatat tcgcagccgc ttaggcgatt cgccagcctt
    15121 tcttctcgtg acaagcaagc aggaagcggc gctgtatgcg atcaacggcg aagatctcgc
    15181 cgagttgggg agcttcgtcg tcgaacgccc ggcagacacc gataaggaag gggagttcaa
    15241 atcgcggagc cgcggcggct cggcgagcgg caccgcctac gagcggaccg acgacgcgca
    15301 gatccgcgac ttcaccagcc tcatggaagt aatggcacgg agcaagctct atggcgcatc
    15361 cggccgactg tacgcattcg cgcccgcggc ggtgaagagc catctcatga aggcgcttgg
    15421 cggctcggcc cgctcgctcg aagtgatttt taccgaagga aattacatgc acagttcgcc
    15481 ggtcgacctt cttgataaga tcgcgcgcga attcgaatcg aagcgcgtcg ttccgaccga
    15541 tccggcggcg cagagaattc tcgacattcc catctagaat ccgtttcaag cgctgagcga
    15601 aaatcggaaa aatttttccg gcaccctgcg ccgcatgaag gctaactcat aaaaaatatt
    15661 tttattttct aaggcggagc ttggctccgc tttttcatat cgctgtcatc tttgcttcac
    15721 agaatttaca cctggaaata ttacttttga tgcaacaaac gtattcactt accatgacgg
    15781 caacaacatt ttcatccgct tctcgtctcg attaaagctc aggcttgcct caatgcgcgc
    15841 gcgttgggac aagcgtgagc tttattcaaa aaagcccgcg aggtcgatac gatgacctgt
    15901 tctgctgctc aatgtatgta agcagaacag gtcatcgcga atgaatatcc aaatttataa
    15961 gaacggatgg cgcagtgcgc catcgattaa gaatacaaac acaatgggag atcttacacg
    16021 aaaaatcgtt gcggctgcct cgatcgcatc gaccatcgct ttctacagcg ctggagtcgc
    16081 ggccgcaaag acgaatgacg atatccagag cgtgcgcgtc gtaagcagca attccgcttc
    16141 ggtcacgaat tccgcctcgg tgaacgcttc gaccggcggc aatgtcgcga atggcggcaa
    16201 cgcaggaatg ggcggagcgg gcggcaacgg cggtacgacg ggagacgccg ctatcatggg
    16261 ccttcatgtc gctaacggcg ggaacgccgg caacggcgga gcgggaggcg catcgggcga
    16321 cgccggaacg ggcggcgact cgggtgcggc aagctctgtc ggaaccgcgg gtcgcgcttc
    16381 taatggcggc gacagcggaa cggctgacgc tttgagcacg tcgggcgacg ccggcaacgg
    16441 cggcgattca ggcatgggcg gagccggagg cgaaggcggc aacggcggag caggcggaac
    16501 caccggcagc cagactcttg atgtcaccgg caccgcccgc ggcggcaacg gcggcgaagg
    16561 aggcaacggc ggagcgggag gcgttatcgt cacgggcgac gcgggagcgg acattacgat
    16621 gaacaatgac gtcaactcga gcgacacgcg cgtaaacgat cctgctcaaa gcaccgagga
    16681 cctcaacacc tcgaacagca tcgatcgcaa tcacagcatt acggtccgga gcgagaacgg
    16741 atcggttctc gacaatatct tcacggcgat gcttcgcacc ggaaacaaca ccgcgaacgc
    16801 gggaaatgga aatgacggcg gggcagccgg aaacggcggc tcgaccggtg gtgcatatat
    16861 cggaactgcc gcattcgcct cgggcggcaa tgccggaacc ggaggtgacg gagcattgag
    16921 cggcatgggc ggcaacggcg gaacctcggg cattgccgat tcggcaagca acggaggaaa
    16981 tgccaaccag ggcggcactt cgggcgacgc gatctcgacg agcctcaccg gccagggcgg
    17041 caacggcggc gcttcgggtg atgccggggc cggaggcgaa ggcggcaacg gagcccaggg
    17101 cggcacgacc ggcgaccaga gccttaccgc atcctctgac gtgaacggcg gcaacggcgg
    17161 cgaaggaggc aacggcgggg aaggcggcag cattctcacc ggcgcttcgg tagccaggac
    17221 aagcttcata aacgtcatca atcgcgccat tacgcgcgta acgaggtaat ttctatctca
    17281 aatggaaaag tcggagagtg tgctctccgc tttccgcaag cttccggaat tcgaatagcg
    17341 ccggaagctt gcgggaggca tatcaattcc catcaatccc atgaacgagc atgtcatatt
    17401 gcaaacccgc agtgtcattc cggccgtagc gctctttacg gtgcttttcg gcgcggggct
    17461 ctatgcggga aatctgttcg cggcgacaac aagcggcaat gtgtcgtccc gggcgactgt
    17521 ctatgctttt tcgtcgaacg gggcggatgg cgcggacggg caagacggcg cgccgggccg
    17581 cgacggtatt tccggacaga acggggaatc gagcgcgtcc gtgaagatcg ttacgaccgt
    17641 gaacggacaa gtggtgcaga atatcgacga gcatcgcgcg tcggatgacg gttctccggt
    17701 ggtgatcgat atttccacga ccacttccta tgattcggga aatgggcatg caggggattc
    17761 gggcgacgcg ccgcccgcag cgcgggaagg cgagtctgtc gacgtttccg cctcagctga
    17821 tgcgatcgca tcggatgaga cggcagcggc gacatcggag acggcgactt cttcggacga
    17881 agtttccggc tttgtcggtt tttattcgtt cataagatca attttcagcc atgtcgcatc
    17941 attcttccat cttgcatagg gcgagagccg gggccgctct ctttcttgcc gcggcaatga
    18001 tacttttcac gccatactca gtatcattca gcgtccgagt gacgaacgcc gatgaggaag
    18061 cctccagctc gtctagcact tccgatattt ccgaaaccgc aggggcgtct tcggctgata
    18121 cgaatgatgc gaccgccgcg cctgaaacgg cgataagctc cggcgcttcg accgtctctt
    18181 ccgaagcggg caacaatctc aacaccaact cagtgagcgt tggcgatgcg accgatactc
    18241 ctcccgacga taatgccgga ggcgatgaga ctgccacgtc gagtggcgaa agcgcgacga
    18301 cctccgacgc tacgtcgacc gtttcggaca attcggacaa tgcggctacg acctccgatg
    18361 ccacgacgag cttaagcgtc gtaaacgaca gcacggctga cgcgagctcg acgatggatc
    18421 tcgattccag taccggcgcg aacgcggcga gctcgaccgg cgacgtcatg gtgcaatccg
    18481 gcgacgccat cgccaacgcg agccttctca acgtcgtgaa taccaacctg gtgaattcct
    18541 acggggagct tctcgccctt tcaagccttc tcatgaattc ggtcaaccag ttcgatttgc
    18601 ggtcgctcga tttcttcgcc tcatcatcta tccgctgcgc gatctgcggc ggcacgactc
    18661 ttggcagcct ctcggttctc aataacggca cggccagcat atccaacgcg ctctcggctc
    18721 atgctacttc cggcgggaac gccgccgatt cggcaagcgg caccgccgat attctcggcg
    18781 gggacgctta tgcggcgggc aacgtcatca atttcgccaa caataatctc gtcgattcga
    18841 agtacctttt catgacatac aacaacttcg gcgattgggg aggcgatatc gtattcccga
    18901 acgcggaaac gctcatgaaa attctcctct cgggcgcatc tatctctccc tcgaatctct
    18961 cgctttccaa taccggcttc gcggacgtcg ataacaacgt cagcaccacc gcgacatcag
    19021 ggaacaattc gttcacggga ggcacatcga ccgtttcgag cggctcgtcc gacgccacga
    19081 cgaacatcct gaacctcctg aactccaacc tggtcggcgg cgcttccatg aacgtcctgc
    19141 tcaatatcga gggctcatgg tcgggctcga tcatcggcct tccgcctggc gtcgaatgga
    19201 aaaagacgga tcatggcatc gccctctcct tcgattcgat cgaagggtcc gatgatgcga
    19261 ccgattcgcc cgacaccgac gtctcgaacg gcgcgacgac cggtatcggc aactcgatcg
    19321 acgtaagcgc ggcttccggc gacaaccacg cgtccggagc ggacggaagc gtggagagcg
    19381 gggacgccta tgccgcggcg aacgtcatca acgtggcgaa taccaacatg atcggccgca
    19441 acatgttctg ggcgatcatc aatatcttcg gcgactggtc gggagacgtt tccttcggca
    19501 agcccgacct ctggatcggc ggccatgccg acattacggg gccgtccgtg tcgccgggag
    19561 gtgaagtgac atatcatttc acggttatca ataacggcga cctgcctgcg acgggagtat
    19621 ccgtgcacac cgatctcggc tccgactatt tgaaggatga gggcacggac gcgagcacca
    19681 tcctcaccga tctcggcact atcgccccca aggagagcaa gagcttcgat attaccgccc
    19741 gcctcgaccg ttcgatggcc ggcaaggaaa ccgccatcac gagcgccaaa gtgacgagta
    19801 tcgagcacga caacaacctc gacgacaata cggaagacat ctcggttctc ttgggctccc
    19861 cgaggcatca tggaagctcg aggcctaaga cggaagaagc cacttcgaca gcatcgacca
    19921 cgccgcttct cgacgtcagt gcaagcttcg acgggccctc gatcgtcccc gcatcgagca
    19981 ccgtcgcata tcacgtggtc atcaagaatg acggcgatgc tccggtctac aacgccgaat
    20041 tccatgagcg cctgctcgac ggcaccggcg cctcgcttca cggcgaagta tgggatctcg
    20101 gcacgatcgc gccgcacgac gagctcaata tagattattc ggtcttctat gacccggata
    20161 cgccttcggg agtctacacc gcatcgggct tcgtccaggg cacggccggg gaagatgcgg
    20221 catccgctcc cgtggcatcg aacgtttcgg cggtgtcgct catggttcgc ggcctccatc
    20281 tcgttccggc accggggaag gcgccgctcg tcacgaagcc tacaatttcc acgcagggca
    20341 cgagctcttc gtcgaccgca gaaacggccg cgtcggccgc gccttccgac gaacctcaga
    20401 atccgattcc tcctgcgaac gatcgcggcc ttctcgcgtc ggtcttcttc tccgcgccgt
    20461 cggcgggaga gctcaccggc ttctccctcg cgcttctcgc cgcggccgtg ggcatcatgc
    20521 tctatcgcag gcggcagtac gcataggggc ggctcgaacg cttcttctcg cgagattcct
    20581 cgggtttcgt atatactagg aagatgatga ggctagcggc acacgatgaa taagaaggcg
    20641 cttgttgcga tcatcgcggt tttcgccgca ttcggcggat tgcttctgtg ggggctcggc
    20701 ataacgccct cggagcatat tctcgcgaac atctcgagtt tcgaggattg cgtgagcgcg
    20761 ggcctcgact cgaccggcac cccgccctcg tgtgctacgc ccgacggacg caccttcgcg
    20821 aaactcggcg ctcctcctgc tccgccgcag gaagcgactt cgactgacat tgcgaccgat
    20881 accgcaccga cgatcgctgt cgacgcgccc cagccggacg atgccgtggt aagtccgctc
    20941 cgagtgagcg gcaaggctcg aggctggtat tttgaagcga cgtttcccgt gcgtctcctc
    21001 gattcgaaag ggagaaccgt cgctgtcggg gcggccgtcg cgtcgggcga ttggacgagc
    21061 cggacggaag tcccgtttac cgcccggctc acgttctccg cgccttcgac cgagacaggc
    21121 acgctcgttc tcgagaggag caattcttcc ggattgccgg atcagagcgc ggaagtgagc
    21181 attccggtga gattttcgac cgtggcgggc gaagcgactt ctaccgcaac gacgtcgtca
    21241 ggtgaagggg cggcgacggg aagccctgcc gaaacccgct aagcggcagc ttttcattcg
    21301 aaatcgcgta gcggggaatg gcagaaatga tacaatagat cccatggaca gtttggcaaa
    21361 atccgacatt ttctttttca tcacgacgat agcggtcgtc attctcacga tcctcgcgtc
    21421 gatcgcgctt gtctacgcga tcaagtttct tcgcgatctc cgcgtcaggg gcagggaagt
    21481 c
//
LOCUS       DASZYS010000057        13934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_509863, whole genome shotgun sequence.
ACCESSION   DASZYS010000057 DASZYS010000000
VERSION     DASZYS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509863"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..2109
                     /locus_tag="VHF05_03420"
     CDS             172..2109
                     /locus_tag="VHF05_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80007.1"
                     /translation="MDDNESGRIERLRRRLYSRKNPLDFQDSRSDIKPEQFEVQSEWK
                     EEEGAAPIGLALHARDKKKSSFFNRLFIVSLIFFLACVAVAAYVFFGGLNRISPNNVD
                     IVVAGPVSVAAGNALPLDIIVKDNNALNLEDVSITVHFPDSTRSPDNLTEPLTTYTED
                     IGEVPAGGSVHRTVTGVFFGQKDDVQNVTVTATYKIKASTATYNKEKTYQIAIQSSPI
                     IMTVSYPKEVNVGGDTEFDIDVSSNTNTDLNNMVLTADYPFGFVFASSDPAPTGGQNT
                     WNIGTLKPLEKRTIKLHGTLEAEDNEERTFTFHAGTADPSDSGKIGVNLSTTQESLTV
                     KKPEIELGIVWNGSKEALAVPASNRIQADIDYKNNLPVNLVNANIVAKISGPILDQQS
                     VIPTYNGFFRSLDNTVTWDMNGLKDLAQVAPGADGTVGLTFASLPTTADLISKVKNPQ
                     ITLDVTMTGSRVDSGGVPQTVTATLSKVVQVTTELGITQRAVYSVGPFQNTGPIPPKA
                     NTETTYTVIWDASNTFNDVSGAKVTAQLPPYVKWKGVISPSTEGVTYNALTNSVTWNI
                     GNLPRGTGYTTSPKEVMFQIGMTPSITQVGTRPTLVNTVSISGADTFTKQQAAKTAPE
                     VTTLLSTDPNVDAKNTSEVAQ"
     gene            2252..3616
                     /locus_tag="VHF05_03425"
     CDS             2252..3616
                     /locus_tag="VHF05_03425"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HVS80008.1"
                     /translation="MNDLMEKIVSLCKRRGFIFQGSEIYGGLAGTWDFGPYGVALKNN
                     VKNLWWKRFVLDREDMYGIDADILMNAAVWEASGHAFGFSDPLTTDAKTGKKYRADHL
                     LEAAGVDVKGMKIADMSAAIKEKKLKSPDGNTLSDVENFNMMLKTHIGADVNNPTVSY
                     LRPETAQGMFVNFKNVLDTFHPKLPFGLAQIGKAFRNEIAPRDFIFRLRELEQMEIEY
                     FVRPEKWEESFEHFRRQMHEWTRDIGLAEPHVHELEVAPEDRAHYSKRTIDFEFDYPF
                     GKKELYGLAYRTDYDLGKHSEHSGVDLSYFDEETKERFIPHVIEPSFGVDRTILAILS
                     DAYTEDEMGGETRTFLKFSPNVAPVKIAVFPLLKNKPQLVEKAREVYRELKKEFGAVE
                     FDDNGNIGKRYRRQDEIGTPYCVTIDFDTIEKDSTVTIRDRDTGTQERVAIAELKEFL
                     ASRF"
     gene            4142..5410
                     /locus_tag="VHF05_03430"
     CDS             4142..5410
                     /locus_tag="VHF05_03430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVS80009.1"
                     /translation="MRYIKKTLKNGLRIISVPMPDAATVTILVLVEAGSKYETKRING
                     VAHFLEHMCFKGTAKRPAALDISRELDSIGAEYNAYTSQEYTGYYAKSAAKHYGKILD
                     IVSDLYLNPTLDEREIEKEKGVVIEEINMYDDSPSDLVGELYEALLYGDQPAGWPILG
                     TRESVQLLTREDISKYRAAHYEPQSTAVIVAGNFDEKKVFDDLEAAFGGMKPSAKAGK
                     EKVRIDQSEPALLIKNKDSEQAHLALGVRTFSVYDKRALPLRLLNVVLSGGMSSRLWH
                     RIRNEMGVAYYVYSSPSFRTDHGDLTVYAGVDNSRVPEVVAAIIEEFRRLLDEPVSDE
                     ELQKAKDYYAGQLALALESSDEFATFYGHQEIINREIQKPDDIIKELQAITADDLRKV
                     ANAVFDEANLNLALIGRVGDPETLKKLLKI"
     gene            5576..6625
                     /locus_tag="VHF05_03435"
     CDS             5576..6625
                     /locus_tag="VHF05_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVS80010.1"
                     /translation="MQQPEHRIIHDISTVAILKVLAILALCGVLYYLRGIVLIVLVAV
                     VIAAAIEPSTKWFIRHRVPRVAAVIFEYLLLAASLAIIFYFLLIPLIGEAVNFVQTLP
                     NYIQSIEVGGGITGVEGTAPAISLDTITNQLNGAIGDLSHGFFSTLSTVFGGAFSFVL
                     IIVISLYLSVQEKGISRFLSVITPIKQRPYILALWDKSEVKIGRWMQGQVLLAGIIFV
                     MVYLGLLAFGIPHALLLAFFAGLFEIVPVVGPIFAAIPGVLLAFASGGFTFGIVIALF
                     YTLVQQLENHVVYPLVVKKVVGVPPLISIIALLAGGELLGFLGIVIAVPVAAILMEFM
                     DDMQKKDVDTGSSAL"
     gene            6859..8295
                     /gene="metG"
                     /locus_tag="VHF05_03440"
     CDS             6859..8295
                     /gene="metG"
                     /locus_tag="VHF05_03440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVS80011.1"
                     /translation="MDRPETFYITTPIFYPNAKLHMGHAYTTTVSDILARYNRLAGKD
                     AYLLTGSDENTEKLVRAAEAAGKDPKEYLGEIVEGFKDLYAKLGISYDQFIRTTDEER
                     HWRGAQEFWKRLVAAGDIEKRSYEGLYCVGHEAFITEKDLVDGKCPDHNEAPMRLREE
                     NYFFKLSKYTGEIKRKIESGELEILPVARKNEILALLERGLEDISFSRPADKMTLGIP
                     VPDDPSQKIYVWGDALTNYVTALGFGTEDDGLFRKFWPADVHVIGKDILRFHAAIWPG
                     MLLSAGLPLPKRLLVHGFITSGGKKMSKSLGNVVDPLELIGEYGAEAVRYFLARHISP
                     FEDGDLTPDGFKEAYNANLANGLGNLVNRVMKMAESNLDAPVEIRDEGMPREYKIAFE
                     SFNPQAAAEAVWNLIGQADKTIQELQPFKLVKDHPMQAKEIIAGLVQQVYSIGKLLEP
                     LMPETSAAILASVAANKMPEKPLFMRKD"
     gene            8550..9362
                     /locus_tag="VHF05_03445"
     CDS             8550..9362
                     /locus_tag="VHF05_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVS80012.1"
                     /translation="MPAYIDIHSHLNFPQFDADRAEAIQRMRDAGIWTITVGTDRATS
                     ESAMKLAEENDGMFATVGVHPTHEEAMGKDELIALARSSAKVVAIGECGLDYYRKDGT
                     DEAEKKRQREVFETHIEAAIELGLPLMIHGRAAYGDIYDILASYHREHGDKVRANMHF
                     FAGSADDARKFLDFGFSLSFDGPITFANSYDEVIRLSPLEAIMAETDAPFAAPAPFRG
                     RRNEPLYVREVARRIAEIRNDDPEKVREALVKNAVRIFGLEAQEGVARPVSI"
     gene            9376..9867
                     /locus_tag="VHF05_03450"
     CDS             9376..9867
                     /locus_tag="VHF05_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVS80013.1"
                     /translation="MENSAQNAHETEEKVLKVYEVGYHLVPSVPEEQVPAEVGAIKEF
                     IEGQGGLFISDEFPKMRQLAYAIPKVVDGRKQNFEEAYFGWVKFEAPSEAAPAIQKAL
                     DSNPHVLRSLLIETVRENTMSVAKPAFKKDDEKNSASGEEKEITKEDEERISKSIDEL
                     VAE"
     gene            9985..10449
                     /locus_tag="VHF05_03455"
     CDS             9985..10449
                     /locus_tag="VHF05_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVS80014.1"
                     /translation="MYLNKAILIGNLTRDPELKSLPSGIQVASFSIATNRVWRDKNGA
                     KQESADYHNIVVFGRQAETVSRYLKKGSSVLVEGRMQTRSWDAQDGSKKYRTEIIAER
                     VQFGPRREGEGNYGGGAPKAASGADHQADAPMGDEGGAVDYPDEINPDEIPF"
     gene            10633..10839
                     /gene="rpsR"
                     /locus_tag="VHF05_03460"
     CDS             10633..10839
                     /gene="rpsR"
                     /locus_tag="VHF05_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVS80015.1"
                     /translation="MAHKQSYFAQNNIQHIDYKDTELLKKFLTPHARVQSKKRTSVDS
                     KHQRKLDVAVKRARFMALLPYIAR"
     gene            complement(11441..12013)
                     /locus_tag="VHF05_03465"
     CDS             complement(11441..12013)
                     /locus_tag="VHF05_03465"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVS80016.1"
                     /translation="MLEYNEIKEGKVIVFEGEPWVVLTSHVFRKQQRKPVNATKLKNL
                     ITGRVTEQSFHVSEKAEEADIETKDIKYLYTNKGEWWFCEANDPSARFKMTSEEVGAG
                     GRFLKQNSVVQAVLYDEKIIGVKLPIKVELKVIEAHPAVKGNTAQGGSKMVKVETGAE
                     IQVPMFIKEGDVLRINPETGEYTERLGASF"
     gene            complement(12255..12431)
                     /locus_tag="VHF05_03470"
     CDS             complement(12255..12431)
                     /locus_tag="VHF05_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80017.1"
                     /translation="MSLENYGQSKNEHLFSSWQGLENGLSELEEELKKTDPEAAEELE
                     EIIKNFEGLRERIM"
     gene            complement(12447..13475)
                     /gene="recA"
                     /locus_tag="VHF05_03475"
     CDS             complement(12447..13475)
                     /gene="recA"
                     /locus_tag="VHF05_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVS80018.1"
                     /translation="MAKRTKDKNADEQPAARTSSSISDTLREIKTKFGDEAIMTLGEK
                     PKVDIDAIPTGSIGLDAALGVGGMPRGRIVEIFGPESSGKTTLALHIVAEAQKKNGIC
                     AYIDAEHAMDPEYAKKLGVKIDELLISQPDTGEQALEICESLVRSGKIDVVVVDSVAA
                     LTPKDEIEGEMGQAHVGKQARLMSQALRKLTAIVAKSKTVVIFINQIRMQIGVMFGNP
                     ETTPGGKALKFYTSVRLDIRRIAQIKKGEEVMGGRVRVKVVKNKVAPPFRQTEFDLMY
                     NEGISREGEIIALGEKMGIIAKSGTSYKYNDESIGRGYDATRQYLRENGDVANKILDE
                     IRSRLHEL"
     gene            complement(13459..13866)
                     /locus_tag="VHF05_03480"
     CDS             complement(13459..13866)
                     /locus_tag="VHF05_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80019.1"
                     /translation="MRYTPKFILKTSLVAIVCGFVAGYTLFQAQSLLKGPVVTLDQTA
                     RLGGGSDVAEIGGVAKNVAYITFNGRRIYTDTNGDFKEKFALSDGYNIVKITATDKFG
                     RETDKTVELVYDEPAGGDSLSMQTINSTYGKTN"
BASE COUNT         3177 a         3891 c         3792 g         3074 t
ORIGIN      
        1 gaaatgagtt ctaagcgcat gatatacttt ttctatggat cctgttgtag aatttcggat
       61 ttgaacaggt tgagagttag attcccgcaa cagtgccata tatgatgcgc aagcatttca
      121 ttaactacgg aggtgtccgc atgcgcggac gatccgaaat ttcacaacag gatggatgac
      181 aatgagtcag gcaggattga gcgcctgagg cgccgcctct actcgcgcaa aaaccccctc
      241 gatttccaag acagccgatc cgacatcaag ccggagcagt tcgaggtgca aagcgaatgg
      301 aaggaggaag agggcgccgc gcccatcgga ctcgcgcttc atgcccgcga caaaaagaag
      361 agctcttttt tcaacaggct tttcatcgta tcgctcatat tctttctcgc gtgcgtcgcc
      421 gtcgccgcgt atgttttctt cggcggcttg aaccgcatct ccccgaataa cgtcgacatc
      481 gtcgtcgccg gtccggtgtc ggtcgcggcc ggcaacgctt tgccgctcga catcatcgtc
      541 aaggacaaca acgccctgaa tctcgaggac gtgtcgatca ccgtacattt tcccgacagc
      601 acccgctctc ccgacaatct gacggagccg cttaccacgt acactgaaga tatcggggaa
      661 gtgccggcag gcggaagcgt gcaccgcacc gtcacgggcg tgttcttcgg acagaaggac
      721 gacgtgcaga acgtcacggt aacagcgacg tacaagatca aggcgtcgac cgcaacctac
      781 aacaaggaga agacctatca gatcgccatc cagtcgtcgc ccatcatcat gaccgtttcg
      841 tacccgaagg aggtcaacgt gggaggggat acggaattcg atatcgacgt aagttcgaac
      901 accaataccg atctcaacaa catggttctt acagccgatt atcccttcgg cttcgtgttc
      961 gcgagttccg atcccgcgcc gaccggcggc cagaatacat ggaatatcgg cacgttgaag
     1021 ccccttgaga agcgcacgat caagctccat ggcacgctcg aggcggagga caacgaggag
     1081 cgcactttca ccttccatgc cggaaccgcc gatccgagcg acagcggcaa gatcggtgtc
     1141 aatctctcga cgactcagga atcgctcacc gtgaagaagc cggaaatcga gcttggcatc
     1201 gtctggaacg gcagcaagga agcgctcgcc gtgcccgctt cgaacaggat tcaggccgat
     1261 atcgactaca agaacaatct gccggtcaat ctggtgaatg cgaatatcgt cgcgaagatt
     1321 tcgggcccca ttctcgacca gcagtcggtg attcccacct acaacggctt cttccgctcg
     1381 ctcgacaata ctgtcacctg ggatatgaac ggcttgaagg acctcgccca ggtcgcaccg
     1441 ggcgccgacg gtacggtcgg gctcacgttc gcgagccttc cgaccaccgc cgatctcatt
     1501 tcaaaggtca agaatccgca gatcacgctt gacgtcacca tgacgggaag ccgcgtcgat
     1561 tcgggcggcg tgccgcaaac cgttacggcc acgctctcca aggtggtgca ggtgactacc
     1621 gagctcggca ttacccagcg cgcggtctat tcggtcggcc cgttccaaaa taccggcccc
     1681 ataccgccga aggccaatac cgaaaccaca tacacggtga tttgggatgc gtcgaacacc
     1741 ttcaacgacg tctcgggcgc caaggtaacg gcccagcttc cgccgtacgt gaagtggaag
     1801 ggagtgataa gcccgtcgac cgaaggcgtg acgtacaatg cgctcaccaa ttccgtcacg
     1861 tggaatatcg gcaatctgcc gcggggaacc ggctatacga cgtcgcccaa ggaagtcatg
     1921 tttcaaatcg gcatgacgcc gagcatcacc caggtgggaa cacgcccgac tctcgtcaat
     1981 acggtttcca taagcggggc ggacacgttt accaagcagc aggccgcgaa gaccgccccc
     2041 gaggtgacga cccttctttc gaccgatccg aatgtcgacg ccaaaaacac cagcgaagtg
     2101 gcgcagtagc gcgcttgcat gcgcctcccc gataggatag ctttagaccc atggcacaag
     2161 aatcagacaa aatggagaaa atcgtctcgc tcgccaaccc cgcaggaaac gctatgcgtt
     2221 ccaacggggc agggcgtcgc tcacaacaac tatgaacgac ttaatggaaa aaatagtatc
     2281 tttgtgcaaa cgccgaggtt ttatattcca aggctccgaa atctatggcg ggcttgctgg
     2341 cacgtgggac ttcggcccgt acggcgtggc gctcaagaac aacgtgaaga atctctggtg
     2401 gaagcgtttc gtgctcgatc gtgaggatat gtatggcatt gatgccgata ttttgatgaa
     2461 cgcggcggtg tgggaagcct ccggccatgc gttcggattc tccgatccgc ttacgacgga
     2521 tgcgaaaacc ggcaagaagt accgcgccga ccatcttctc gaggccgcgg gcgtcgacgt
     2581 gaagggaatg aagattgccg acatgagcgc ggcgatcaag gaaaagaaac tgaagagtcc
     2641 tgacggcaac acgctttccg atgtggaaaa tttcaatatg atgctcaaaa cgcacatcgg
     2701 cgcggacgtg aacaatccga cggtttccta tcttcgcccc gaaacggcgc agggcatgtt
     2761 cgtgaatttc aagaacgtgc tcgatacgtt ccatcccaag cttccttttg gcctcgccca
     2821 gatcggcaag gcattcagga atgaaattgc cccgcgcgat ttcattttcc gcctccgcga
     2881 gctcgagcag atggaaatcg agtattttgt gcggcctgaa aagtgggaag aatcgttcga
     2941 gcattttcgc cggcagatgc acgagtggac ccgcgacatc ggcctcgccg agccgcacgt
     3001 gcacgagctc gaagtagcac ccgaagatcg ggcacactat tccaagcgca ctatcgattt
     3061 cgaattcgac tatccgttcg gcaagaagga gctgtacggt ctcgcgtacc gcaccgatta
     3121 cgatctcggc aagcactcgg agcacagcgg tgtcgatctc tcgtacttcg acgaggagac
     3181 gaaggagcgg ttcattccgc atgtgatcga gccgtcattc ggtgtcgacc gcacgattct
     3241 tgcgatcctt tccgacgcgt ataccgaaga cgaaatggga ggcgagactc gcacgttcct
     3301 gaaattctct cccaacgttg cgccggtgaa gattgccgtc tttccgcttc tcaaaaacaa
     3361 gcctcagctc gtcgagaagg cgagggaagt ctatcgcgaa cttaaaaaag aattcggcgc
     3421 cgtcgaattc gacgacaacg gcaatatcgg caagcgctac cgccgccagg acgagatcgg
     3481 cacgccctac tgcgtgacga tcgacttcga tacgatcgag aaggattcga ccgttaccat
     3541 tcgcgaccgc gacaccggca cccaggagcg cgtcgcaatt gcggagctga aagagttttt
     3601 agcctctaga ttctagttgc tagtggtttg tcattgcgag aaatcttcga cgaagcaatc
     3661 actcctctct gtcatcccga gtacgggatc cagggcttcg attttataaa agccagaaga
     3721 cccggggttc gtattgctcc actgcaattt gaaatttgac agataaaata ttttatattt
     3781 atgtatatca aggagacagc aatgatcttt aagtgtgagt cgtatgaaag tctgcaaaag
     3841 aaggcgagcc aatggcatcg gagttttgcg ttattgccaa aacgcgtcgg tactgaccaa
     3901 aatggcaaag gtatttgggc ctggcttcaa tggatcgaag taagagagga aaaatcgaca
     3961 ctttaccgtc ggcttcccaa ttctgatgca ggctatagta aagcaatata ttagttattt
     4021 cgagccgctt ggtttccagg cggctccatc tttttatata aacttggttt tgtgctactc
     4081 tagctatgct ttctgttctc aggctaaaga ctagcaacta acaactagaa actgaatttc
     4141 tatgcgttac ataaagaaaa cattaaaaaa cggtcttcga attatctccg tgccgatgcc
     4201 cgacgccgcg acagtgacga tcctcgtcct ggtcgaggcg ggatcgaagt acgaaacgaa
     4261 gcgcataaac ggcgtggcgc atttcctcga gcacatgtgc ttcaagggaa cggcgaagag
     4321 gcctgcggca ttggacatca gccgcgagct cgactcgatc ggcgccgaat acaacgcgta
     4381 cacttcgcag gaatataccg gctactacgc caagtcggcg gccaagcact acggcaagat
     4441 tctcgacatc gtctccgacc tctatctcaa tccgacgctc gacgagcgcg agatcgaaaa
     4501 ggagaagggc gtggtgatcg aggagatcaa catgtacgac gattctccgt cggatctcgt
     4561 cggcgagctc tacgaagcgc ttctctacgg cgaccagccg gccggatggc cgattctcgg
     4621 cacccgcgaa tccgtccagc ttcttacgcg cgaggacatt tccaagtacc gcgcggcgca
     4681 ctacgagcct cagtcgactg ccgtgatcgt tgcgggaaat ttcgatgaga aaaaagtttt
     4741 cgacgatctt gaagcggcat tcggcggcat gaagccgtcc gcaaaggcgg ggaaggagaa
     4801 agtgagaatc gaccagagcg agccggcgct tctcatcaag aacaaggatt ccgaacaggc
     4861 gcatctcgcg ctgggcgtgc gcacgttttc cgtctacgac aagcgggcct tgccgctccg
     4921 gctcttgaat gtcgtccttt ccggcggcat gagctcgcgc ctctggcaca ggatccgcaa
     4981 cgaaatgggc gtagcgtact atgtgtactc gtcgccgagc ttcaggacgg atcacggcga
     5041 ccttacggtc tacgcgggcg tcgacaacag ccgcgtgccg gaagtggttg cggcaatcat
     5101 cgaggaattc aggcggcttc tcgacgagcc ggtctcggac gaggagctcc agaaagcgaa
     5161 ggactactat gccggccagc tcgccctcgc cctcgaatcg tccgacgagt tcgccacctt
     5221 ctacggccat caggaaatca tcaatcgcga gatccagaag ccggacgaca tcatcaagga
     5281 gcttcaggcc ataaccgccg acgacctgcg caaggtcgcc aatgccgtct ttgacgaggc
     5341 gaacctcaac ctggccctca tcggccgcgt aggcgacccc gagaccttga agaaactcct
     5401 caaaatatag ctccgcggct gtgttcagtt cgggggaggg atggtatcat aaaaggatgc
     5461 agtcccgtta gaggccgcgg ccgcttgatg ggatcatata actcaatggt cgaaacattt
     5521 gaacaaaata atctttttgc aggaagcgta ttgaacgcga cctgcctcta acgggatgca
     5581 gcagcccgaa catcgcatta tccatgacat cagcacggtg gcgatactca aggttctcgc
     5641 catcctcgct ctctgcggag tgctctacta tctgcgcggc atcgtgctca tcgtgctcgt
     5701 ggccgtcgtt atcgcggcgg ccatcgagcc gtcgacaaag tggttcatta ggcatagggt
     5761 gccgcgcgtg gcggcggtca ttttcgaata tctcctgctc gccgcatccc ttgcgatcat
     5821 cttctatttc ctcctcatcc ctctcatcgg cgaggcggtc aatttcgttc agacgcttcc
     5881 gaattacatc cagtcgatcg aggtcggcgg gggaataacc ggcgtcgaag gcaccgcgcc
     5941 cgccatctcg ctcgatacga tcaccaatca gctgaacggc gccatcgggg acctctccca
     6001 tggctttttc tcgacgctct cgaccgtgtt cggcggcgcg ttcagcttcg tgcttatcat
     6061 cgtcatctcg ctctaccttt cggtgcagga aaaaggaatt tctcgattcc tttcggtcat
     6121 tacgcccata aaacagcgcc cgtacattct cgccttgtgg gacaaatccg aagtgaagat
     6181 cggccgctgg atgcaagggc aggttctcct cgcgggcatc attttcgtca tggtctatct
     6241 ggggctcctg gcgttcggca ttccgcacgc gctccttctc gcgttcttcg ccggcctttt
     6301 cgagatcgtg ccggtggtcg gcccgatctt cgcggcgatt cccggcgtcc tgctcgcgtt
     6361 cgcttcgggc ggcttcacct tcggcatcgt catcgccctc ttctacacgc tcgtgcagca
     6421 gcttgaaaat cacgtcgtct atccgctcgt ggtgaagaag gtcgtcggcg ttccgccgct
     6481 tatctccatt atcgcccttc tcgcgggagg ggagcttctc ggtttcctcg gcatcgtcat
     6541 cgcggttccg gtcgccgcca ttctcatgga gttcatggac gacatgcaga agaaggacgt
     6601 tgacaccggc tcctcggctc tgtaacgtgt ctcacatgga tcccgttaga ggccgcgatc
     6661 acgtgcctca ttcacaatgg tcgaaatact tggaaaaaga aatcatatta attttgcgga
     6721 aagcgcggtg agcatgatct gtcccgttta gaaatctacg aaccatgaat tacaagctaa
     6781 tcaaattcaa gcagagcaaa gctggaaacg agcattcgac cgaatgtgag atttctaacg
     6841 ggatctgcct ctaacgggat ggatcggccc gaaactttct atatcaccac tcctattttc
     6901 tatcccaacg cgaagctcca catggggcac gcgtatacga cgaccgtctc cgatattctt
     6961 gcgagataca accgccttgc gggcaaggac gcgtacctcc tcaccggctc cgacgagaat
     7021 acggaaaagc tcgtacgggc cgcagaggcg gccggcaagg atccgaagga gtatctcggc
     7081 gagatcgtgg agggcttcaa ggatctctac gcgaagctcg gcatttcata cgatcagttt
     7141 atccgcacga ccgacgagga gcggcactgg agaggagcgc aggaattctg gaagcggctc
     7201 gtcgcggcgg gcgacatcga gaagcgctcg tacgaggggc tctattgcgt cgggcacgag
     7261 gcgttcatta ccgagaagga ccttgtcgac ggcaagtgcc ccgatcacaa cgaagcgccg
     7321 atgcgcctca gggaggaaaa ttatttcttc aagctttcaa aatacaccgg cgagatcaaa
     7381 cggaagatcg agagcgggga attggaaatc ctgcccgtcg cccgcaagaa tgagattctc
     7441 gcccttctcg agcgcggcct cgaagacata agcttctcgc gcccggccga caaaatgacg
     7501 ctcggaatac ccgtacccga cgatccgtcg cagaagatct acgtctgggg cgacgctctc
     7561 acgaactatg tcaccgcgct cggcttcggc accgaggacg acggcctttt caggaaattc
     7621 tggccggccg acgtgcatgt gatcggcaag gatattctcc gctttcatgc ggcgatctgg
     7681 ccgggcatgc ttctctccgc cggcttgccg cttcccaagc gcctgctcgt ccacggattc
     7741 atcacctcgg gcggcaagaa gatgtcgaag tcgctcggca acgtcgtcga tccgctcgag
     7801 cttatcggtg agtacggggc ggaagcagtg cgctattttc tcgcgcgcca catttctccg
     7861 ttcgaggacg gcgacctcac gcctgatggc ttcaaggaag cgtacaatgc gaacctcgcg
     7921 aatggtctgg gcaatctcgt gaaccgcgtg atgaaaatgg ccgagagcaa tctcgacgct
     7981 cctgtcgaga ttcgcgacga aggaatgccg cgggaataca aaattgcctt cgaatcgttt
     8041 aatccccaag cggccgccga ggccgtgtgg aatctcatcg gccaggccga caagacgatc
     8101 caggaactcc agccgttcaa gctcgtgaag gatcatccca tgcaggcgaa ggagatcatc
     8161 gccggacttg tccagcaggt ctatagcatc ggcaagctcc tcgagccttt gatgcccgaa
     8221 acgagcgccg cgattctcgc ttccgtcgct gcaaacaaga tgcccgaaaa gccgctcttt
     8281 atgaggaaag actagaataa aacccccgcc tgcagacggg ggtggaaagt tttacaactt
     8341 cagtattatc cagtttgaga ccggcacgtg attgccaaac tctgccacaa gacaagcact
     8401 aaacgctaac accgaagacc tgaattccga gcagcaaagc ttgaattcaa gtaagaggcg
     8461 ctgagaagta gtacgacatc ttctgctcgc aagcaaccct ctctcggcct tcatgcagta
     8521 tatgccgtct atccgataga aacaaattta tgcccgctta catcgacatt cattctcatt
     8581 tgaatttccc gcaattcgac gccgatcgcg ccgaggctat tcagcgcatg agggatgcgg
     8641 gtatctggac gatcaccgtc gggacggatc gcgccacgtc ggaatcggcc atgaagctcg
     8701 ccgaagaaaa cgacggaatg ttcgcgacgg tgggcgttca tcccacgcac gaagaggcga
     8761 tggggaagga tgagctcatt gcgctcgcgc gttcgagcgc aaaagttgtg gccatcggcg
     8821 aatgcggcct cgactactac cgcaaggacg gcaccgatga ggcggagaag aagcgccaga
     8881 gagaagtttt tgaaacgcac attgaggccg cgatcgagct cggtcttccg ctcatgattc
     8941 atggccgcgc ggcttacggc gatatttacg atatccttgc gtcgtaccac cgggagcacg
     9001 gcgacaaagt tcgggccaat atgcacttct ttgcagggag cgcggatgac gcccgcaagt
     9061 ttctcgactt cggcttctcc ctttcctttg atggccctat aactttcgcg aattcgtacg
     9121 acgaggtgat ccgactctcg ccgcttgagg caattatggc cgagacagac gcgccgttcg
     9181 ccgccccagc gccctttcgc ggccgccgca atgagccgct ctatgtgcgt gaagtagccc
     9241 gccgcatcgc cgagatccgc aatgatgatc cggagaaggt acgggaggca ttggtcaaaa
     9301 atgccgtgag aatcttcggt ttggaggctc aggaaggggt tgcacgcccc gtatccatat
     9361 gatagagtgt tcgccatgga aaatagcgca caaaacgcac acgaaaccga agagaaggtc
     9421 ttaaaagtct acgaggtggg ctaccatctc gtgccctcag ttcccgaaga gcaggttccg
     9481 gccgaggtcg gagcgatcaa ggagttcatc gaggggcagg gaggactttt catttccgac
     9541 gagttcccga agatgaggca gcttgcatac gcgatcccca aggtcgtcga tggcaggaag
     9601 cagaacttcg aggaggccta cttcggatgg gtgaagttcg aagctccttc cgaggccgcc
     9661 cctgcgatcc agaaggcgct tgattcgaac ccgcacgtgc tccgctcgct tctcatcgaa
     9721 acggttcgcg agaataccat gagcgtcgcg aagcccgcgt tcaagaagga cgacgaaaag
     9781 aattcggctt ccggcgagga gaaggagatc accaaggagg acgaggagag gatcagcaaa
     9841 tcgatcgacg agctcgtggc ggagtaatgg gagattcggg gtttaggagg tatgatgtaa
     9901 gagtaggggc atctcccatt caagaatcat aaaatcgtac ctgcctgccg gtaggcggga
     9961 atcctaacca aaccaatcaa aaacatgtac ttaaacaagg ccattctcat cggaaatctt
    10021 acccgcgatc cggaactcaa gtcattgccg tcaggcatcc aggtcgcttc gttttcgatc
    10081 gccaccaaca gggtatggcg cgacaagaac ggcgccaagc aggaatcggc cgattaccac
    10141 aatatcgtgg ttttcggacg ccaggcggag acggtcagcc gctatctcaa gaagggaagc
    10201 tcggtccttg tcgaaggcag gatgcagacg agaagctggg atgcccagga cggttccaag
    10261 aagtaccgca ccgagattat cgccgagcgc gtccagttcg gcccgaggcg cgaaggcgag
    10321 ggaaattacg gcggaggcgc cccgaaagcc gcttccggcg cggatcatca ggctgatgcc
    10381 ccaatgggcg atgagggagg ggcggtggat tatccggatg agatcaatcc cgacgaaata
    10441 cccttttaaa cagttcttcc aaattgagcg cattccattg tcgccgcctg tgtttgctcc
    10501 cgcgcagtac tagcatgtac agcttggtcg caagcctcgc cggcttcgcg gactgcatct
    10561 caatttgaaa gccctggaac tattgcattt taaggaacaa tcctgtataa agaaacaacc
    10621 caaaaaaccg atatggcaca caagcagtct tatttcgcac aaaacaatat ccagcacatc
    10681 gactataagg acacggagct tttgaagaag ttcctcactc cgcatgcccg cgtccagtcg
    10741 aagaagcgca ccagcgttga ttccaagcat cagcgcaagc tcgatgtcgc ggtgaagagg
    10801 gcgcgcttta tggcccttct cccatatatc gctcgataaa atcctcaggt cggcgcactt
    10861 ccgcgttgct catttgcgtt tgcttgaagc agtattgctt atacagctcc tgcgcaagcc
    10921 gcactcgcgc cttgaattgc atccaacctg atgattttag aaatacaaaa ccggccgaaa
    10981 ggccggtttt gttgtttgac atactctcat ttcaaggtaa ggtttgagat ggagggcgtt
    11041 atgctcataa aagtaagaca ctttgaactg gagggtgagg agcgtatgca gccattcggc
    11101 caagatatga ttcctgtaaa cgggcagtta tcgaacatat ttctgttggc acggaagaaa
    11161 acccgggagt tatgccaaaa aggcgacagg tttttttaca atcatcttat tggatgataa
    11221 caatcagatg ctttattact cgcatccaat cgacgacctg aatcctcggc gcgggtacac
    11281 agaacggttg cctcagggaa gattgggaat aatcttaccg gatggggaaa tgccgcttcc
    11341 tgaacatgac gagccgcatg acgtacttct gaattaaaaa atgcggtgcg acccgtgggt
    11401 cgcaccgcat tttcttttac tcgaaactat aaactagcaa ctagaaacta gctcccaacc
    11461 tttcggtata ctctcccgtc tcggggttga tgcggagcac gtcgccttcc ttgatgaaca
    11521 tcggcacctg gatttccgct ccggtctcga ctttcaccat cttggagccg ccctgggcgg
    11581 tgtttccttt gaccgccgga tgggcctcga ttaccttgag ctcaaccttg atggggagct
    11641 ttacgccgat gatcttctca tcgtagagaa ccgcctggac gacggagttc tgcttgagaa
    11701 aacggccgcc cgcgccgact tcttcggagg tcatcttgaa gcgtgccgac ggatcgttcg
    11761 cttcgcagaa ccaccactct cccttattgg tgtagaggta cttgatatcc ttggtctcga
    11821 tatcggcttc ctcggccttt tcagaaacgt ggaacgactg ctcggttacg cggccggtga
    11881 tcagattctt cagcttcgtg gcattcacgg gcttcctctg ctgcttgcgg aagacgtggg
    11941 aggtgagtac cacccacggt tcgccttcga agacgataac ttttccttcc ttgatttcgt
    12001 tgtattccag catggttgaa ttttaaggag cgaagcgact attaaattcg ccacccagca
    12061 ccatttctga tatgaaagct tgttgattat tgagcacaaa attgctcttg cgcttttaag
    12121 caatcaatta tcgtcagttt cagaaatggt gctgggtcaa gaaaatacac tcgcttcact
    12181 cgttattttc taagacgcct gtcagtctag cacaccgcgg caaaagaaaa aaccccggcc
    12241 tttcatgccg gggattacat gatgcgctcg cgaaggccct caaaattctt gatgatttcc
    12301 tccaattctt ccgccgcttc gggatcggtt ttcttgagtt cttcttcgag ttcgctcaat
    12361 ccgttttcaa ggccctgcca actgctgaac aaatgctcgt ttttgctttg gccgtaattc
    12421 tcgagggaca tggaattttt ttatatttat aactcgtgga gcctgctgcg gatttcgtcg
    12481 aggatcttgt ttgcgacatc gccgttctcg cggagatatt ggcgggtggc gtcgtagccg
    12541 cggccgatcg attcgtcatt gtatttgtat gacgttccgg atttcgcgat gatgcccatt
    12601 ttttcgccga gtgcgatgat ctcgccctcg cgggaaatgc cctcgttgta catgagatcg
    12661 aattccgtct ggcggaacgg gggagcgacc ttattcttca cgactttgac tcgtacgcgt
    12721 ccgcccatca cttcttcgcc tttcttgatt tgggcgattc tgcggatatc gagacgcacc
    12781 gacgtgtaga acttgagcgc cttgccgccc ggcgtggtct cggggttgcc gaacatgacg
    12841 ccgatctgca tgcggatctg gttgatgaaa atgacgaccg tcttcgactt ggcgacgatg
    12901 gccgtgagtt tgcggagggc ctggctcatg agcctggcct gcttgccgac atgggcttgg
    12961 cccatttcgc cttcgatctc gtctttcggg gtaagagccg ccacggagtc gacgacgacg
    13021 acgtcgatct tgcccgagcg tacaaggctt tcgcagattt cgagcgcctg ctcgccggta
    13081 tcgggctggg agatgagaag ctcgtcgatc ttcacgccga gcttcttggc gtactcggga
    13141 tccatggcgt gctccgcgtc gatgtacgcg caaatgccgt ttttcttctg ggcttcggcg
    13201 acgatgtgaa gggcgagagt agtcttacct gacgattcgg gtccgaagat ttcgacgatc
    13261 ctgccgcgcg gcatgccgcc gacgccgagg gccgcgtcga ggccgatgga gccggtgggg
    13321 atcgcgtcga tatcgacctt cggcttctcg cccagggtca tgatcgcctc gtcgccgaat
    13381 tttgtcttga tttcgcgcag ggtatcgctg atgctcgagc tggttcgggc tgccggctgt
    13441 tcgtccgcat tcttgtcttt agttcgtttt gccataagtg ctgttgatgg tctgcattga
    13501 taatgaatct cctcccgccg gttcgtcgta cacgagttcc accgtcttgt ctgtctccct
    13561 gccgaatttg tccgtcgctg ttatcttcac gatgttgtac ccgtcggaaa gcgcgaactt
    13621 ttctttgaaa tcgccgttcg tgtccgtata gatgcgcctg ccgttgaagg tgatgtaggc
    13681 gacgttcttc gccacgccgc cgatctcggc gacatcgctt ccgccgccga gtcgcgctgt
    13741 ctggtcgagg gtgacgaccg ggcctttcaa aaggctctgc gcctggaaaa gcgtatagcc
    13801 ggctacgaac ccgcatacta tagcgacaag ggatgttttt agtatgaact tcggcgtata
    13861 acgcatatgg atattatacc tcttttagcc cgcaaacttc attccgcatt cattttgctc
    13921 actccgcgct ctcg
//
LOCUS       DASZYS010000058         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_831168, whole genome shotgun sequence.
ACCESSION   DASZYS010000058 DASZYS010000000
VERSION     DASZYS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831168"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1129
                     /locus_tag="VHF05_03485"
     CDS             47..1129
                     /locus_tag="VHF05_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVS80020.1"
                     /translation="MQDALIIVLVALIAALAAFLWLLYRKLSKLETGASDGQSLNLVL
                     QQINEINRTVDMKLGESHKLMSSSVNSQFSESQRLVNQFIKEVTAMTDKVARVEETGK
                     QMMSIADQLNSLEDILKNPKQRGILGEYYLEALLKNVLPPGSYQMQYPFADGTIVDAA
                     VFVKDKIIPIDSKFSLENYNRLAEERSEAERERLEKLFVSDLKLRIQETSKYIQPGQS
                     TMDFAFMFIPHEAIYYDLLINKIGGLKDDNENLIQRAAAKYRVIIVSPTSFLAYLQTV
                     LQGLKALQIEERAAQIIKNVEDLGKHLKAYEEYHGKLGTALGTVVNHYNKSSGEFKKI
                     DKDMVRIGNDSLELGIDAVEKPYLDD"
     gene            1422..1793
                     /gene="rplU"
                     /locus_tag="VHF05_03490"
     CDS             1422..1793
                     /gene="rplU"
                     /locus_tag="VHF05_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013311592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVS80021.1"
                     /translation="MAPKKTTKKTAKAAEQGGEFAIIETGGKQYKVASGDVLKIEKLK
                     GDFKEGDTITFDKVLLVDNGTDETTIGTPYISGAAVTARFEKAGNRDKVTVIKYKAKS
                     RYFKKNGHKQPYFQVRIEGIK"
     gene            complement(1870..2085)
                     /locus_tag="VHF05_03495"
     CDS             complement(1870..2085)
                     /locus_tag="VHF05_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80022.1"
                     /translation="MAERDETGPDFQMGHVDPAECGVQPHVIGEEEEFVRKAAEVGWS
                     EEQASLLWKTFAPKGHSHDGLRLIGFW"
     gene            complement(2420..3034)
                     /locus_tag="VHF05_03500"
     CDS             complement(2420..3034)
                     /locus_tag="VHF05_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVS80023.1"
                     /translation="MNSIQRLAELLSELPGIGPRQSKRIVYHLLTRRRDFLNELSDRV
                     RTIADSIAECASCHRFYEKGSGNGDLCSICGDPHRDRSLLMIVARDADLDSMEKAKGY
                     NGLYFVLGGTVPILEKEPRKTIREDELLKTIERYASTLKEIIFALNANPEGENTETYL
                     TRELDPQKEKYSLGFSKLGRGLSTGSELEYADKNTLQNALQNRH"
     gene            complement(3176..>4053)
                     /locus_tag="VHF05_03505"
     CDS             complement(3176..>4053)
                     /locus_tag="VHF05_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HVS80024.1"
                     /translation="LAEAWERIDRLHNSSGELRGIPTGFPELDNITSGFQKSDLLILA
                     ARPSVGKTAFSLDIARRVAVNHNIPVCIFSLEMSAQQLVDRMLAAQAQVDAWKLRTGK
                     LSRDEDFQSLADALDKLRNAPIYIDDQPGTNIIRMRSVARRLKSEKNLGLLIVDYLQL
                     MVPTQSRNSDNVVQQVTEISRSLKNLARELDIPVLALSQLSRAVETRGGKPKLSDLRD
                     SGSIEQDADIVMFIHRDANDDGGGKKQEAEILIEKHRNGMTGKIDLFFNDKKATFQSV
                     DKTNYSGSDKEFNDF"
BASE COUNT          990 a         1065 c         1116 g          882 t
ORIGIN      
        1 ataaacgcat tttccgatac acttctccct ctgatataat tacgcaatgc aagacgctct
       61 cataatcgtt ttagtcgccc taatcgccgc cttggcggca tttctctggc tgctctaccg
      121 caagctttcc aagctcgaaa cgggcgcctc cgacgggcag agcctcaatc tcgtcctcca
      181 gcagatcaac gagatcaacc gcaccgtcga catgaagctc ggagagtcgc acaagctcat
      241 gtcgagctcc gtcaacagcc agttctcgga gtctcagcgg ctggtcaatc agttcatcaa
      301 ggaagtcacg gccatgaccg acaaggtggc gcgcgtcgag gaaacgggga agcagatgat
      361 gtcgatcgcc gaccagctga actcgctcga ggacatcctg aagaatccca aacagcgcgg
      421 aatcctcggc gaatactatc tcgaagcgct cctcaagaac gttctgccgc ccgggagcta
      481 ccagatgcag tatcccttcg ccgacggcac gatcgtcgat gcggccgtat tcgtgaagga
      541 caagatcatc ccgatcgact cgaaattctc gctcgagaac tacaaccgcc tggcggaaga
      601 gaggagcgag gcggagcgtg agcggctcga gaagcttttc gtgagcgatt tgaagctccg
      661 cattcaggaa acctcgaaat atatccagcc gggccagagc acgatggatt tcgcattcat
      721 gttcatcccg cacgaggcga tctactacga cctccttatc aacaagatcg ggggcttgaa
      781 ggacgataac gaaaacctca tccagcgcgc cgccgcgaag taccgcgtca tcatcgtttc
      841 gccgacgtcc ttcctcgcgt atctccagac cgtgcttcag ggcctcaagg cgctccagat
      901 cgaggagcgg gcggcgcaga tcatcaagaa cgtcgaggac ctcggaaagc acctcaaggc
      961 ctacgaggaa taccacggca agctcggcac cgcgctcggc acggtggtca accactacaa
     1021 caagtcgagc ggcgagttca agaagatcga caaggacatg gtccgcatcg gcaacgacag
     1081 cctcgaactc ggcatcgacg cggtcgagaa gccgtatctc gacgactaat gtccacaagc
     1141 ggaaactgct gaggtttgat atagttgaag gagtagtaaa attcccagaa aggagacctt
     1201 attatgaaga atccatgccg atacgatata aacattggca acccgcgcgg agatacttct
     1261 gagtaaggga ggtgatcctt ttctagggct gcctaatccg cagccaaagc cccgaatcgg
     1321 ctgattcggg gctttctaac ggaataagta ccgcaacact tgcgtttttc gtatatttat
     1381 atagaatgtt ctgcatcgta aattaaggag tttaaaagat catggcaccg aaaaagacaa
     1441 ccaaaaagac cgcaaaggca gccgaacagg gcggcgaatt cgcgatcatc gagacggggg
     1501 gcaagcagta caaggttgct tccggcgacg tgttgaaaat cgagaaattg aaaggcgact
     1561 tcaaggaagg agacacgatc accttcgaca aagtcctcct cgtcgacaat ggtacggacg
     1621 agaccacgat cggcactcct tacatctccg gcgcggcagt tactgcccgt ttcgaaaagg
     1681 ccggcaaccg cgacaaggta accgtcatca agtacaaggc caagagccgc tacttcaaga
     1741 agaacggcca caagcagccc tacttccagg taagaatcga ggggatcaag taaggctgca
     1801 aggaaggaaa aatttataac ctgaaaacaa aaagcgcacc acataggtgc gcttcggcat
     1861 ttgcatgccc taccaaaaac cgattagcct gaggccgtca tgcgaatggc ccttcggggc
     1921 gaatgtcttc catagaaggg aagcttgctc ctcgctccaa ccgacctccg ccgctttgcg
     1981 tacgaactct tcttcttcgc ctataacatg cggttggaca ccgcactcag ccggatcgac
     2041 atggcccatt tgaaaatcag gtccggtttc atctctttct gccatacgtg ctaatcctcc
     2101 agggctgttt ataccatttc ggcggagcga tagctagtgt ttcttgacaa ttattttaaa
     2161 catgatatac taccgacagt tatgaagttt tacttcctcc tcaacatttg cgagggcgcc
     2221 acaggacatc tcgcaagcct ttggagcacc tgttccacat ttctggaaaa tcagatcggc
     2281 aatcaagtcg atctcgccca cacacttctg cgactgcatt acttgtggtt gccgcaacta
     2341 tggagccttg tcctgaagct cctgtaatac agacgcttct aagcgcaaag cccgcctgac
     2401 ccggcgggct tttttatact cagtggcgat tttgcagggc gttctgaaga gtattcttat
     2461 ccgcgtactc aagctcgctt ccggtggaga gcccgcgccc gagcttggaa aagcccaggg
     2521 aatatttctc tttctgagga tcgagctcgc gcgtgagata ggtttcggta ttttctccct
     2581 ccgggttggc attcaaggcg aagattatct ccttcaaggt cgaggcgtac cgctcgatcg
     2641 ttttcaaaag ctcatcctcg cggatcgtct tgcgcggctc cttctcgagg ataggcaccg
     2701 tgccgcctag aacgaagtag aggccgttgt agcccttggc cttctccatg gaatcgagat
     2761 cggcatcgcg cgcgacgatc atgagaagcg agcgatcgcg gtgcgggtcg ccgcagatgg
     2821 agcagagatc gccgttgccg gagccttttt catagaagcg gtggcaggaa gcgcattcgg
     2881 ctattgagtc ggcgatggtg cgcacgcggt cggaaagctc gttcaaaaaa tcgcggcggc
     2941 gggtgagaag atggtagacg atgcgcttcg actgccgagg cccgatgccc ggaagctcgg
     3001 agagaagttc tgcaaggcgc tggattgaat tcatcagaaa attgtagcaa ttttcgataa
     3061 attttcaatt tgcaattctc aatttccaat aaattttcaa tgaaacaatt ttaaatgctt
     3121 gagacattgg aatttgaaaa ttcattggaa attgtaaatt gaaaatttat ttttcttaaa
     3181 agtcattaaa ctccttatcg gaacccgaat agttcgtctt atccacgctc tggaaggtag
     3241 ccttcttgtc gttgaagaag aggtcgattt tgccggtcat gccgtttcgg tgcttctcga
     3301 tgagaatttc ggcctcctgc tttttgccgc cgccgtcgtc attggcgtcg cggtggatga
     3361 acatgacaat gtccgcgtcc tgctcgatag agccggaatc gcgaaggtcg gagagcttcg
     3421 gcttgccgcc gcgcgtttcg acggcgcggg agagctggga aagagcgagg acgggaatgt
     3481 cgagctcgcg ggcaagattt ttgagcgagc gtgaaatttc ggtaacctgc tggacgacgt
     3541 tgtcgctgtt gcgggactgg gtggggacca tgagctggag gtagtcgaca atgagaagac
     3601 cgagattctt ctcgctcttc aagcgcctgg caacggagcg catacggatg atgttggtgc
     3661 ccggctggtc gtcaatataa atcggcgcgt tgcggagctt gtcgagggca tcggcaagcg
     3721 actggaagtc ctcgtcgcga gagagctttc cggtgcgcag cttccaagca tccacctgcg
     3781 cctgagcggc gagcatgcgg tcgacgagct gctgggcgct catttcgagc gagaaaatgc
     3841 agacgggaat attgtgattt acggccacgc ggcgggcaat atcgagcgag aatgcggtct
     3901 taccgacgga aggacgggct gcgagaatta ggagatcgga tttctggaag cccgaagtga
     3961 tgttgtcgag ctcggggaag ccggtgggga tgccgcgcaa ttcgccggag gaattgtgca
     4021 ggcggtcgat gcgctcccac gcttcggcaa gcg
//
LOCUS       DASZYS010000059         7890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_574071, whole genome shotgun sequence.
ACCESSION   DASZYS010000059 DASZYS010000000
VERSION     DASZYS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574071"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1098)
                     /locus_tag="VHF05_03510"
     CDS             complement(241..1098)
                     /locus_tag="VHF05_03510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017555266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVS80025.1"
                     /translation="MLTYFQAIIIGALQGLTELFPVSSLGHSIILPALFGWHLDENAE
                     FFLIFLIATHLATSLVLLGFFWKDWVRVVKGIVLSVIRRRIDPADTYAKLGWLLVIGT
                     IPAGILGLLFQDQFKSLFSSPVVVSLFLGLNGILLLCAELLRRKKAAGEDAQADEQIA
                     AMGFSKAVKIGFAQALALFPGFSRTGSTLGGGLLVGLDHESSARFAFLLATPIILAAA
                     VLKLPELIHNGGSYPIAPVVIGALISAIAAYLSVRFLTKYFKTKTLMPFAIYCLATGV
                     LSALILLVR"
     gene            complement(1295..2068)
                     /locus_tag="VHF05_03515"
     CDS             complement(1295..2068)
                     /locus_tag="VHF05_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HVS80026.1"
                     /translation="MSTPIFEAFISVFLVSAASLAGIAALFTAEKDRNGITFALVSLS
                     VGVLFGDALLHILPDLFASGESVIALASLVFAGIMLFFVLEKYLHVDHEHGFHEYGEE
                     AHRHGLRNRALGTTILAADIIHNFLDGLIVGASYLGGAKLGLATTLAVVFHEIPHEMG
                     NVGVLLHAGYSKKKAFWYNTLTALTAFFGLACAVFFGQALAGFTDYALAFAAGGFIYI
                     AGSELVPELREESGFRPSLVQFACMILGFGIMALVLLLE"
     gene            2181..2993
                     /locus_tag="VHF05_03520"
     CDS             2181..2993
                     /locus_tag="VHF05_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVS80027.1"
                     /translation="MPADHGNKRSAVSDNKYLVPLSIIIAGLIVAGAVYFSRTPTSAP
                     AAGTQNQPQQQNTDATIRPVDPAKDHILGDPNAPIELVEYSDAQCPFCRAFHPTITSI
                     MSTYGKDGTVNWVYREFPLDSIHPDAHRRALGLECAAQEGGNSAFWSLLNSLFAIDDN
                     ANEGTPDLGAMAKQAGLDETAFNTCLNNKAAESLVAADESDALQAGAQGTPFIVMVFK
                     NGVNSATKNAVSSLQAKYSPDVVYVSTDGKRVVIGGALPLADFQTILDAATK"
     gene            complement(3025..3414)
                     /locus_tag="VHF05_03525"
     CDS             complement(3025..3414)
                     /locus_tag="VHF05_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80028.1"
                     /translation="MRQLREKHVALSDEQLTALIAARRVASARCLYPSREVVHIPSLV
                     ETNRGLRKSIQVTLDYPHKAQSCFAIDLRPLEAGGRKIWVPDHMAEIFHVDMGAAQTK
                     EIEYDFSTEHPQEPYEYVPAMKEKSPS"
     gene            complement(3499..3867)
                     /locus_tag="VHF05_03530"
     CDS             complement(3499..3867)
                     /locus_tag="VHF05_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80029.1"
                     /translation="MVNSIVAGVIESAALLAARSLISELDLEQAQPALLGIRKTEENF
                     RQNFLRIIFFSLSTRRRSLKNCIAIMLQLERIDGCRIWYAVAGSLCVQKKKGVWLFQN
                     YGMEPVLEGSKVIGYQVDSP"
     gene            complement(3927..7340)
                     /locus_tag="VHF05_03535"
     CDS             complement(3927..7340)
                     /locus_tag="VHF05_03535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HVS80030.1"
                     /translation="MTTEAFEKVYRKLNKAQKEAVDTIEGPVMVIAGPGSGKTQILSL
                     RVANILLKTDTRPSNILCLTFTDSAAVNMRKRLSEMIGPDAYRVAIYTFHSFGTDIIE
                     RYPEHFFQGARFAPADDLAQIEVLEEILEELPHDNPLRSVHPEQGFTYLSDLKQAIGS
                     LKKAGIAPVEFKAALAHIKDEAVAADAILSPLFDARLSMKLVAELRKGAEEVRAIDSG
                     TFPLPHFAPYADTLADALDSALDDAEEMGKTEPLSRLKSSLFGKTDDGGRILKDLANM
                     EKMESLADIYEKYRVRMYEAGAYDFDDMILEVIQAVESDAGLRAELQEQYQFVLVDEF
                     QDTNDAQMRLLRGLLDAEVNEGRPNIMVVGDDDQAIYKFQGAELSNILNFRSQYKEPK
                     IVTMAHNYRSTQVILDIAKLVIERGEERLENILPEVEKDLVAANDQIAKGDIRSKTFP
                     TIGHEYRFIAKEIRRLLDAGEEANNIAVIARKHAFLEGLVPYLNDARVPIRYERQSDV
                     LKEPHVRELIVMARFATSLMKNEREEADHLLPEILSFPFWGVSRKTIWQISVDADRSR
                     RLWLEIMLDHEDENIRRIAEFFIDLSQIALYDTMERVVDILIGSHLALAADDEYDDEA
                     VATDVVDILNPGQSQKSVFISPFKEYYWSIDRLKESKADYLIFLSSLRTFVAAVRGYK
                     RGELAKLEDLVAFVDLHEKNRLVIPDTSAFANGLDAISLVTAHKAKGLEWQTVFVINC
                     RNDVWAGRGPGRKLSFPLNLPITPAGDTVDDQLRLFYVALTRAKRNLYLTASATTESG
                     KEALRLQFLFPRDGSKAGSANSDLAKLLEFAEESFDMDETIEALATATSSLSRNPLLY
                     DEEAFLKTLVENYCMSVTHMNNFLNVAKGGPAYFFEQNLLRFPQEPNLSSKFGTAMHA
                     LIERVYNHLKFEGGMPEQKTLLSWLDRELRQFRMTERDFELLRERGRKSIASYIEGKK
                     DSFKPTDKIEVNFRNQGVALNGAELTGKIDKMVFKEGGAKEKWSVHDFKTGRALADWD
                     GKDDYDRTKLHNYKYQLIFYKLLVENSREFCDDCVAEEGCLEFIEPIRGKIVELPLAF
                     EPGDVERVKKLAEIVYKKIVALDFPDVSGYDQNVNGILAFEEDLLEGKI"
     gene            7406..>7890
                     /locus_tag="VHF05_03540"
     CDS             7406..>7890
                     /locus_tag="VHF05_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80031.1"
                     /translation="METEEVRLKTILIVLMLSLQLGAQNIERVSKKDISPVDVNYEIF
                     KSLVANLAVYSGKQEMLVESAKVPVLRLDAPACKRALIAYDKNRADIWAAIEKVENQA
                     SKLEASMRDAIKTGGTPPGKCILIEKEIFRAANLIEGGSHQPDTMMRARIIVDHDEKP
                     H"
BASE COUNT         1803 a         2258 c         2167 g         1662 t
ORIGIN      
        1 gcccatgttt ctgccgctcc cgcggcagtc actccgagca ttgagactaa tgcccgcaaa
       61 gggccagcta agcccgcaaa cggcctgccg atgccgcacc ccataccctg cccctggtgg
      121 attccggggt gcagcgccca aaagtagggc aaaaaaagca attgcgattg tcttttcgca
      181 aacaaaggcg gacccggcaa cggtccgctt ttttcatatt aaaaattcca aaccagaagg
      241 ttaccgtaca agaagaatga gcgccgagag aacgcccgta gcgaggcaat agatggcgaa
      301 cggcatgagg gttttggttt tgaaatattt cgtaaggaaa cgcacggaga gatacgccgc
      361 gattgccgat atgagcgctc cgataacaac gggggcaatc gggtagctcc ctccgttatg
      421 aataagctcg ggaagcttca aaacggccgc ggcgaggatg atcggcgtgg cgaggaggaa
      481 cgcgaaacgc gccgaggact cgtgatcgag cccgacgaga aggccgccgc caagcgttga
      541 gccggtgcgc gagaagccgg ggaaaagcgc gagcgcctgg gcgaagccga tcttcaccgc
      601 cttggaaaat cccatcgcgg caatctgctc gtcggcttgc gcatcttccc ctgccgcctt
      661 tttgcgcctc agaagctcgg cgcaaagaag gagaatgccg ttcaatccca aaaaaagcga
      721 aaccacgacc ggcgacgaga aaaggctttt gaattgatcc tggaaaagaa gcccgagaat
      781 tccggcggga atcgtgccga taacgagaag ccagccgagc ttcgcgtacg tatcggcggg
      841 atcgatgcgc cgccgaataa ccgaaagaac gatgcctttg accaccctca cccagtcttt
      901 ccagaaaaat ccgagaagga caagcgatgt ggcaagatgc gtcgcgatga ggaaaatgag
      961 aaagaactcg gcattttcat cgagatgcca gccgaaaaga gccggcaaga tgatgctgtg
     1021 gccgagactc gatacgggaa aaagctcagt gagtccctgc agcgccccga ttataattgc
     1081 ctgaaaatag gtaagcatac gatcaattta aaacgttact cgacgatcac ggatattttt
     1141 ctgctgaaaa attccgcgac cccggctcgg cccgtcggcc tccgcccttg cgaagcgctc
     1201 gcccccaatc agaattgcgg gttccctaac gcgcttctca ccctgcagcg ccccgattat
     1261 tattgcttga aaataggtga gcatatcgat gagtttactc caaaagaagc acgagcgcca
     1321 tgattccgaa gccgaggatc atgcatgcga actggacgag cgacggcctg aagcccgatt
     1381 cctcccgcaa ttccggcacg agctccgaac cggcaatgta tataaagccg ccggcggcaa
     1441 acgcgagggc gtagtcggta aagcccgcga gcgcctgccc gaaaaatacc gcgcatgcca
     1501 ggccgaaaaa agctgtgaga gcggtcaggg tgttgtacca aaacgccttt ttcttggaat
     1561 agcccgcatg aagaagcacg ccgacgtttc ccatctcgtg cgggatctca tgaaagacca
     1621 cggcaagggt cgttgcgagg ccgagcttcg ctccgccgag gtaggaggcg ccgacgataa
     1681 ggccatcgag aaaattgtgg atgatatccg cggcaagtat cgtggtcccg agcgcgcgat
     1741 tcctcagccc gtgacgatgg gcttcctccc cgtattcatg aaagccgtgc tcgtgatcga
     1801 catgcaggta tttttccagc acgaagaaaa gcatgatgcc cgcaaagacg agcgatgcaa
     1861 gcgcaatgac actttcgccg gaagcgaaaa gatcaggaag aatgtggaga agagcatcgc
     1921 cgaacaatac gcctaccgac aagctcacga gcgcgaacgt gattccgttc ctgtctttct
     1981 cggcggtaaa aagcgccgcg atgccggcga gcgacgccgc actcactaaa aatacgctta
     2041 tgaacgcctc gaaaatcgga gtactcattg ccattacgat accacaggtt gctcatctgc
     2101 ggcaggagca tatcgcctgc tatccaaata tgctagtatg gatcatacat ataaattaac
     2161 gcagagctat gcaagaaaac atgcccgcgg atcacggcaa taagcggtcc gcagtgtccg
     2221 acaacaagta tctcgttccc ctttcgataa taatcgccgg cctcatcgtg gccggagccg
     2281 tatacttctc gcgcaccccg acgagcgcgc ctgccgcggg cacccagaac cagccgcagc
     2341 agcagaatac cgatgccacg atccgtcccg tcgatccggc gaaggatcac atcttgggtg
     2401 atcccaatgc gccgatcgag ctcgtcgagt actccgacgc gcagtgcccg ttctgccgcg
     2461 cattccaccc gactatcacc tcgatcatgt cgacctacgg caaggacggc acggtcaact
     2521 gggtataccg cgaattcccg ctcgacagca tccatcccga cgcgcaccgg cgcgccctgg
     2581 gcctcgaatg cgccgcgcag gagggcggca attcggcctt ctggtcgctc ctcaacagcc
     2641 tcttcgcgat cgatgacaat gccaacgaag gtacgcccga tctcggcgcc atggcgaagc
     2701 aggcaggcct cgatgaaacc gcattcaaca cctgcctcaa caacaaggcg gccgaaagcc
     2761 tcgtggcggc ggatgaaagc gacgcgctcc aggccggcgc ccaaggcacg ccgttcatcg
     2821 tcatggtatt caagaacggc gtgaatagcg cgacgaagaa tgccgtctcg agcctccagg
     2881 cgaagtacag cccggatgtc gtctacgtat cgaccgacgg caagcgggtc gtcatcggcg
     2941 gcgcgcttcc tctcgccgat ttccagacca tcctcgacgc cgcgacgaaa tagccgcaaa
     3001 aaaactccat tgtaaaagcc cgtctcatga cgggcttttc tctttcatag caggaacgta
     3061 ttcgtacggc tcctgaggat gttcggttga aaaatcgtac tcaatctctt tcgtttgcgc
     3121 ggcgcccata tcgacgtgaa atatttcggc catatgatcg ggcacccaga ttttccgtcc
     3181 tcccgcttca agcggtcgca gatcaatcgc gaaacacgac tgggctttgt gtggataatc
     3241 caaagtaacc tgaatcgatt ttctaagccc ccggttcgtt tccacgagag agggaatgtg
     3301 aacgacctct cgagaggggt acaggcacct cgcgcttgcg actctccgtg ccgctatgag
     3361 tgcggttaac tgttcgtccg ataaggcgac gtgcttttcc ctgagctgcc tcattattat
     3421 ttcaatgcct ccatgactaa aataacataa tttttaatat ctggcaataa aaaatgcccg
     3481 ctgaaacgag gcgggcattc atggggaatc tacctgatag ccgatgacct tagagccttc
     3541 cagcaccggc tccatgccgt agttctggaa cagccagacg ccttttttct tttgcacgca
     3601 caggcttccc gccaccgcgt accatatcct gcaaccatcg atccgctcca gctggagcat
     3661 gatcgcgatg caattcttga ggcttctcct tcttgtcgaa agggagaaga atattattcg
     3721 aagaaaattc tgacggaaat tttcttcggt ttttcgtatg cccaggagag ctggctgcgc
     3781 ctgctccaga tcgagctcgc ttatgaggct tcgtgcggca agtagcgccg cggattcgat
     3841 tacccccgca actatgctgt tcaccatttc gaacacctcc aatcaaaaac taccccgaat
     3901 ttatgaaaat ggcaagaatg agggaattaa atcttccctt ccaagagatc ttcctcgaac
     3961 gcgagaatgc cgttcacgtt ctggtcgtag ccggacacat cgggaaaatc gagcgcgacg
     4021 attttcttgt agacaatctc ggcgagcttc ttcacccgct ccacgtctcc cggctcgaat
     4081 gcgagcggca gttcgacgat tttccccctg atcggctcga tgaattcgag acagccttct
     4141 tcggcaacgc agtcgtcgca aaactcccgc gagttctcga cgagaagctt gtagaaaata
     4201 agctggtact tgtagttgtg gagcttcgtg cggtcgtagt cgtccttgcc atcccagtcc
     4261 gcgagggctc ggccggtctt gaaatcatgg acgctccact tctccttcgc accgccttcc
     4321 ttgaacacca tcttgtcaat cttgccggta agctccgcgc cgtttaaggc gacaccctga
     4381 ttcctgaagt tcacttcgat cttgtcggta ggcttgaacg aatccttctt cccttcgata
     4441 tacgaagcga tgcttttcct tcccctttct ctcaagagct cgaaatcccg ctccgtcatg
     4501 cggaattgcc tgagttcgcg atcgagccat gagaggagcg tcttctgctc cggcatgccg
     4561 ccttcgaatt tcaaatgatt gtagacccgc tcgataaggg cgtgcatcgc ggtgccgaac
     4621 ttgctcgaaa ggttcggctc ctgcgggaag cggaggagat tctgctcgaa gaaatacgcc
     4681 gggccgccct ttgcgacgtt caaaaaatta ttcatatgcg tcacgctcat gcagtagttc
     4741 tcgaccaaag tcttcaagaa cgcttcttcg tcgtagagca gaggattgcg gctcaatgag
     4801 cttgtcgcag ttgcaagcgc ttcgatcgtc tcatccatgt cgaacgactc ctcggcaaac
     4861 tcgagaagct ttgcaagatc ggaattggcc gacccggcct tcgatccatc ccgcgggaag
     4921 aggaattgaa gccgcaaagc ttccttgccg ctctcggtcg tggctgacgc cgtaagatag
     4981 agattgcgct tggcgcgggt aagggcgacg taaaaaaggc ggagctggtc gtcgacggtg
     5041 tcgccggcgg gcgttatcgg gagattgagc ggaaacgaaa gcttcctgcc cgggccgcgg
     5101 ccggcccaga cgtcgttgcg gcaattgatg acgaataccg tctgccattc gaggcccttg
     5161 gccttgtggg cggtcacgag cgatatcgcg tcgaggccgt tcgcgaaggc cgaggtatcg
     5221 ggaatgacaa gccggttctt ttcgtggagg tcgacgaacg cgacgagatc ctcgagcttc
     5281 gccaattctc cccgcttgta tccgcgcacc gccgcgacga acgtgcggag gctcgagagg
     5341 aagatgagat agtcagcctt gctctccttc aggcggtcga tgctccagta gtattccttg
     5401 aagggcgaga tgaatacgct tttctgcgac tggccgggat tcagaatgtc gacgacatcg
     5461 gttgcaaccg cctcgtcgtc gtactcgtcg tccgcggcaa gggcgaggtg cgagccgatg
     5521 aggatgtcta cgacgcgttc catcgtgtcg taaagcgcga tttgcgagag atcgatgaag
     5581 aactcggcga tacgacggat attctcgtcc tcgtgatcga gcatgatctc gagccagagg
     5641 cgccgcgatc tatcggcatc gacggagatc tgccagatcg tcttgcgcga cacgccccaa
     5701 aacggaaacg agaggatttc cggaagcaga tggtcggcct cttcccgctc gtttttcatg
     5761 agcgacgtgg cgaagcgcgc cataacaatg agctcacgga catgcggctc cttgagcacg
     5821 tccgactgcc gctcgtagcg gatcggcacg cgcgcgtcat tgagatacgg cacgagccct
     5881 tcgagaaagg cgtgcttgcg cgcgatgacg gcgatattgt tcgcttcttc gcccgcatcg
     5941 agcaggcggc ggatttcctt ggcgataaag cggtattcat ggccgatcgt cggaaatgtc
     6001 tttgaacgta tgtcgccctt cgcgatttga tcattcgcgg caacaagatc cttctcaacc
     6061 tccggcagga tgttctcgag ccgctcctcg ccgcgctcga tgacgagttt cgcgatgtcg
     6121 agaatgacct gcgtcgagcg gtagttgtgc gccatagtaa cgatcttcgg ctccttgtat
     6181 tgcgaacgga agttcaggat gttcgagagc tcggcgcctt ggaacttgta gattgcttgg
     6241 tcatcgtcgc ccaccaccat gatattgggc cttccttcgt tcacttccgc atcgagaagg
     6301 ccgcgcagaa ggcgcatttg ggcgtcgttc gtgtcctgga actcatcgac caggacgaac
     6361 tggtattgct cttggagttc ggcgcgaagt cccgcgtcgg actcaacggc ctggatcacc
     6421 tcgaggatca tgtcgtcgaa gtcatatgcg cccgcctcgt acatgcgcac gcggtatttc
     6481 tcatagatgt cggcgagtga ctccattttc tccatatttg ccaggtcctt gagaatccgc
     6541 ccgccgtcgt ccgtcttgcc gaagaggctc gacttcaagc gcgagagagg ctccgtcttc
     6601 cccatctcct ccgcgtcgtc gagcgcgctg tcgagcgcgt cggcaagcgt atcggcgtag
     6661 ggcgcgaagt gcggcagagg aaacgtcccc gaatcgatcg cgcgcacttc ttcggcgccc
     6721 ttgcgaagct ccgcgacgag cttcatcgag agccttgcgt cgaagagcgg agaaaggatc
     6781 gcgtcggcgg cgacagcttc gtctttgatg tgcgcgagag ccgccttgaa ttcgacgggg
     6841 gcaatgccgg ccttcttgag gctcccgatc gcctgcttga gatcggagag ataggtgaag
     6901 ccctgctcgg gatgcacgct ccggagcgga ttgtcgtgcg gaagctcttc caaaatttcc
     6961 tcgagtacct cgatctgcgc caagtcatcc gccggagcga acctcgcgcc ctggaagaag
     7021 tgctcgggat atcgctcgat aatgtcggtg ccgaagctgt ggaaagtgta gatcgccacg
     7081 cggtacgcgt cggggccgat catttcggaa aggcgcttgc gcatattgac cgccgccgaa
     7141 tcggtgaacg tgaggcagag aatattcgac ggcctcgtat cggtcttaag gaggatgttc
     7201 gccacgcgaa gcgagagaat ctgcgtcttg ccgctccccg gccccgcaat caccatgacc
     7261 ggcccctcga tcgtatccac ggcctccttc tgggctttat tgagcttcct atacaccttc
     7321 tcgaacgctt cggtagtcat cctgctattt tatcatgccg actttgcgaa gctcatattt
     7381 gacactgatg ggctatcgac gtatcttgga aacagaggag gttcgcttga aaacgattct
     7441 tatcgttctt atgttatcac tgcagctcgg cgcccaaaat attgagcgcg tttcgaaaaa
     7501 agatatttcg ccggttgacg tcaactacga aattttcaaa tctcttgtcg ccaatttggc
     7561 ggtatattca ggtaagcaag aaatgcttgt cgaaagcgcc aaagtgcccg tcctgcgcct
     7621 tgatgctcca gcctgcaagc gtgcgcttat agcgtacgat aaaaacagag cggatatctg
     7681 ggccgccata gaaaaggtgg agaaccaggc atccaaactc gaagccagca tgagagacgc
     7741 cataaaaaca ggcggtactc cgcccggaaa gtgcattctg atcgaaaaag aaatctttcg
     7801 cgccgcaaac ctgatagaag gcggatcgca tcagccggat acgatgatgc gggctcggat
     7861 tatcgtcgac catgatgaaa agccccatta
//
LOCUS       DASZYS010000060        10497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_120554, whole genome shotgun sequence.
ACCESSION   DASZYS010000060 DASZYS010000000
VERSION     DASZYS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120554"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="rseP"
                     /locus_tag="VHF05_03545"
     CDS             <1..1010
                     /gene="rseP"
                     /locus_tag="VHF05_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HVS80032.1"
                     /translation="AGIRVDEFGLGYPPRIWGKKIGETVYSLNWLPFGGFVKIHGEDL
                     EEAARSGPGSERSFAAKPKWIQVAVLIAGILCNFVFAWLVISAGYMGGTPASVDAGYA
                     VSNPKLTILDVVAGSPAAKAGLQAGDEIERVYTQADELRSSAPDDVSAYVASHQDSAI
                     SIEYERGGADATVTMTPAEGIVEGQKAIGISMDSIGTLRLPLFAALWEGLKTAWNMAV
                     AIVAGLWMLISDAFRGHSDLSQVAGPVGIASMVGQARSLGFSYVLSFMSFISINLAII
                     NLIPFPALDGGRILFVIIEAIKRKPISARVAGTLNMVGFTLLIGLMLVVTFNDILRLA
                     R"
     gene            complement(1057..1497)
                     /locus_tag="VHF05_03550"
     CDS             complement(1057..1497)
                     /locus_tag="VHF05_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80033.1"
                     /translation="MLVRVQADHFLRTKWQMELCIGAAVLLTSCILTLSGAARSDLLL
                     YGISTAIFEQYVLHVPMIFWCLLAPFAAQPPLQFLREWEIWKTGERNYRCYRSNREFI
                     GMAILVTSLIIYAGPSIMLLLGLVLHTVIEASKKPDSYSSSAKG"
     gene            1738..3006
                     /locus_tag="VHF05_03555"
     CDS             1738..3006
                     /locus_tag="VHF05_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HVS80034.1"
                     /translation="MRQSELFTRTRKEVPKDEVSKNAELLLRAGFVSKEMAGLYSFLP
                     LGLRVLNKVMAIIREEMDSIGGQEMLMTALQDPEKWQKTNRWADESVDVWFRTELKGG
                     SSLGLANTHEEAIAAIAAQYVQSYKDLPFAAYQFQTKFRNEHRAKSGILRTREFIMKD
                     LYSFAKSQEELDAFYDKAAAAYKRIFERAGLGGTTYMTFASGGAFSKYSHEFQTICDA
                     GEDIIYISEEKGIAVNKEVYTDEVLADLGLKREDLRETKSIEVGNIFKLGTRFSEPLG
                     LMFKDETGKPQPVIMGSYGIGPARLMGTIVETLSDEHGLVWPASVAPFAVHLVAVGEE
                     PQVKERAEELYKDLMDSGVDVLYDDRDLRAGEKFADSDLLGIPWRLVVSARNMAAGTL
                     ELKERKTGTTESVNESEILNSSVMEKFKAN"
     gene            3009..4073
                     /locus_tag="VHF05_03560"
     CDS             3009..4073
                     /locus_tag="VHF05_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVS80035.1"
                     /translation="MADKGIKGIKRLLSNDIGIDLGTANTLVYVRGQGIVLNEPSVVA
                     VNTKSGRVVAVGAQAKQMLGRTPGHITAVSPLVDGVISDFEVTEEMVSYLLNRAQSSS
                     KKLLGPRVVVGVPSGITNVEIRAVRDATRNAGAREVHIIEEPMAAAIGMRLPVKDPVG
                     TMVIDIGGGTTDIAVISLGGVVRSKSLRIAGNRLNSDIISYIRSEFKILIGEKTAENV
                     KIAIGSVLPGDQPMEAAIKGRDLVTGLPREVVITDSDVREATRQSIDTLVESIREVIE
                     TTPPEILADVMVRGVYVAGGGALIKGLAALIEDMIKIPVYTADDPLTAVARGTGVVLE
                     DIGAHADVLIADENELPPKG"
     gene            4051..4857
                     /gene="mreC"
                     /locus_tag="VHF05_03565"
     CDS             4051..4857
                     /gene="mreC"
                     /locus_tag="VHF05_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HVS80036.1"
                     /translation="MNYRLRDSSTKKKSRKVPLIILAAVVLALVATRVFAPAFSSRAI
                     AAIFAPIMRSGDTLARDAAASGDALKSKSDLIAENAELKSELQAAQDELAVSRDLETE
                     NGELRDLLGLVRATKETPALVLSKPPLSPYDSLVIEIGSQYQVAAGDRVYADGDILLG
                     EVAEADGPTAKVRLYSNAGTEIDAVIERTGVPQKVVGAGGGNFSIKAPKEVDVKAGDV
                     LVVPSLGRKVLGVVKQVDSEPTDAFQQVLIKSPVNIFQLKWVTIESGDTP"
     gene            4880..5149
                     /locus_tag="VHF05_03570"
     CDS             4880..5149
                     /locus_tag="VHF05_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80037.1"
                     /translation="MTRAASRIILDGFILASLLYLPWWITALAAAAGCFLFESFFEAV
                     AFGFIADCLYGVPGRFGGTTFEGTLAASVILGLSLYLRPHLRYYK"
     gene            5292..7001
                     /locus_tag="VHF05_03575"
     CDS             5292..7001
                     /locus_tag="VHF05_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVS80038.1"
                     /translation="MLERFSSKVRKIFRRRSRYEGADIAPDEIFLDSSNLPDFDMSQF
                     EGRFEKPIATRALRLLGFFFVLLALAYASRLWFLQIRSGDLYAKMSEENSDKSTVLFA
                     SRGIIYDRNGVELAWNDLNPDDVFPLRKYTTDPGFAHVLGYVTYPGKDKQGNFYEDTY
                     QPVGGIEKSEDGTLRGTNGEQIVEEDALGEDQGGAKTDEPRDGGNVKLSIDSKVESEV
                     YNAIAQTAHDYGFTGGAAIIMDVHTGEILAYTSFPEFDLNTMTNGTNEEKVAQIQAPE
                     TPFLDRVSSGLYTPGSIVKPYMSMAALTEHTIDPNKIIYTTGSLKIPNPYNPGQYSVF
                     NDWKNLGPLDMRHAIAMSSDVYFYEVGGGFGDQKGLGIANIDKYMAMFGFGQKTGFQF
                     SNEKEGVVPSIEWKKKTFGEDWLLGDTYHSSIGQYGWLVTPLQVVRAVASVANGGTLH
                     TPTLLLNDPNEAANDKHLDLNPDYFEIVREGMRLGVQEGVATALNTPAVKIAAKTGTA
                     ELGITKDYVNSWVTGFFPYDNPHYAFAIVMEHGSRYNLIGASFVMRQIIDWMAANTPQ
                     YLQ"
     gene            7746..8222
                     /gene="greA"
                     /locus_tag="VHF05_03580"
     CDS             7746..8222
                     /gene="greA"
                     /locus_tag="VHF05_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HVS80039.1"
                     /translation="MPDNQKEYLTQEKFNELEKELEQLKTVRRKEVAEELEYAKGLGD
                     LSENAEYHEARDRQATVEDRIAKLEDILKRAVIVSEKDHSSDIVNIGSTVTVKKDGAG
                     SGLTYKIVGSEESDMSQGHISIRSPFGESILGKKKGDEFSFKAPSGMLTYKVVEIK"
     gene            8338..9936
                     /gene="lysS"
                     /locus_tag="VHF05_03585"
     CDS             8338..9936
                     /gene="lysS"
                     /locus_tag="VHF05_03585"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVS80040.1"
                     /translation="MFWADKIAEKIALQYKDKIADGKKLIIRDEKTMSGRVHIGSMRG
                     VAIHGIVSEALKERGIENEFFFEINDFDAMDGIPVYLDRETYEPYMGVSLCDIPSPDL
                     TTGQAGGTAKNFAEYFAEEFLGVIKEAGFAPTYYRSSEKYRSGAYNDMIKTALDRAEL
                     IRTIYKETSGSVKGEDWWPLNVRCEKCGKISTTRVLSWDGSEVEYECRDIEWAKGCGI
                     RGRISPLNGNAKLPWKVEWAAKFSVIDVDIEGGGKDHSTRGGSRDVANHISREVFGRK
                     PPFDIPYEFFLIGGKKMSSSKGQGSSAREFADLLPPQLLRYSLLYKDINQQINVDPEG
                     DAIPLLFDGYDRAAGAFWSGKDDDVARLFKLVNQTDPKLLEKRALPRFSTVAYLVQMP
                     HIDIVAEFEKLEGLSYGERDREELEERAKYARIWLDTYAPEDFKFEIKETLPAEAKNF
                     TPEQKAALKKVLEYVKSQAALDGQELHTELHEIRKASGLEAAQFFGALYLCILGKQSG
                     PKAGWFLSVLDRNFLETRLEEAAS"
BASE COUNT         2446 a         2868 c         2995 g         2188 t
ORIGIN      
        1 gagccggcat ccgggtggac gaattcggcc tgggctatcc gccgcgcatc tggggcaaga
       61 aaatagggga gacggtgtac tcgctcaact ggcttccgtt tggcggcttc gtgaaaatcc
      121 acggcgagga cctcgaggaa gcggcgcgaa gcgggcccgg gagcgagaga agcttcgcgg
      181 cgaagccgaa gtggatccag gtagcggttc tcatcgcggg catcctgtgc aatttcgttt
      241 tcgcatggct tgtcatttcg gcgggctaca tgggcggcac gccggcatcc gtcgatgcgg
      301 gctatgccgt ttcgaacccg aagctcacca tccttgatgt ggtcgccggt tctccagcgg
      361 ccaaggcggg ccttcaggca ggagacgaga tcgagagggt ttacacgcag gccgacgaac
      421 tccgctcgtc cgcgcctgac gacgtgtccg cgtacgtggc gtcgcaccag gacagcgcga
      481 tctcgatcga atacgagcgc ggcggcgccg acgcgaccgt caccatgacc ccggcagaag
      541 gcatcgtgga agggcagaaa gcgatcggca tttcgatgga ttcgatcgga accctccggc
      601 tcccgctgtt tgcggcgctt tgggaaggct tgaaaacagc atggaacatg gcggtggcga
      661 tcgtagccgg actctggatg ctcatctccg acgcgttccg cggccactcc gatctctcgc
      721 aggtcgccgg gccggtaggc atcgcgagca tggtggggca agcgcgcagc ctcgggttct
      781 cctatgtcct ctcgttcatg tcgttcattt cgatcaacct tgccatcatc aatctcattc
      841 cctttcccgc gcttgacggc ggccgcatcc tcttcgtgat catcgaggcg atcaagcgca
      901 agccgatcag cgcccgcgtc gccggcacgc tcaacatggt cggctttacg cttcttatcg
      961 gcttgatgct tgtcgttaca ttcaacgata tcctgcggct cgcgagatag gaaaaaagct
     1021 cgtgcaatgc acgagctttt tgttgtaccg aataacctat ccttttgcgg aggaagaata
     1081 agaatcgggc tttttgctcg cctctatgac tgtgtgtaga acgagtccca gaagcagcat
     1141 gatcgatgga cctgcatata tgatgaggga ggttaccagg atggccatcc cgataaactc
     1201 gcgattgctc cggtagcatc tgtaattgcg ctcgccggtt ttccagattt cccattcccg
     1261 caggaattgc agcggaggct gggcggcaaa cggagcgagg aggcaccaga atatcattgg
     1321 tacatgcaag acgtactgtt cgaaaatcgc cgtgcttatc ccgtacagaa gcaaatcgct
     1381 tcgcgcggcg ccggaaagcg tcagaataca cgatgtcagc aagacggcgg ccccgatgca
     1441 taattccatc tgccacttgg tcctcaaaaa atgatctgcc tgaacgcgaa cgagcatttc
     1501 ttattctccc tgagttgaat ttacatctgg ttactactat acatcaaaca tgtatatttg
     1561 tcaataatat tcatattttg agccgtactt tttgacccac cacagccttt cctgtaagat
     1621 ttaggggaag tcgctagttg ctagtttcta gttgctagaa attccggatt ttttgtttgc
     1681 atatatagta gtagaaacct gcctaaaaac taacaactag aaactataaa cttctccatg
     1741 cgccaatcag agctttttac gaggacacgg aaggaagttc cgaaagatga agtctcgaag
     1801 aatgcggagc ttttgctccg cgcgggtttc gtctcgaagg aaatggcagg cctctattcg
     1861 ttcctgccgc tgggattgcg agtgctcaac aaggtgatgg cgatcatccg cgaggagatg
     1921 gattcgatcg gcggccagga gatgctcatg acggcgctcc aggatccgga aaaatggcag
     1981 aagacaaaca ggtgggccga cgagtcggtc gacgtgtggt tccgcaccga actcaagggc
     2041 ggcagcagcc tcggacttgc caatacgcac gaggaggcga tcgccgcgat cgcggcgcag
     2101 tacgtgcagt cgtacaagga tctcccgttc gccgcgtacc agttccagac caagttcagg
     2161 aacgagcacc gcgcgaagtc tggcattctc cgcacgcgcg agttcatcat gaaagacttg
     2221 tactcgttcg cgaagtcgca ggaagagctc gacgcgttct atgacaaggc cgccgccgca
     2281 tacaagagaa tcttcgagcg cgccggcttg ggcggcacga cctacatgac cttcgcgtcc
     2341 ggcggcgctt tctcgaaata cagccacgaa ttccagacga tctgcgacgc gggcgaggac
     2401 atcatctaca tcagcgaaga gaaggggatc gcggtaaaca aggaggtgta caccgacgag
     2461 gtgctcgccg atctcgggct caagcgcgag gatctcaggg aaacgaaatc gatcgaggtc
     2521 ggcaatatct tcaagctcgg cacccgtttt tcggagccct tgggcctcat gttcaaagac
     2581 gagaccggca agccccagcc tgtcatcatg gggagctatg gcatcggccc cgcgcggctc
     2641 atgggcacca tcgtggagac tctctccgac gagcacggac tcgtctggcc cgcatcggtg
     2701 gcgccctttg cggtgcatct cgtcgcggtc ggcgaagagc cgcaggtgaa ggagcgcgcc
     2761 gaggagcttt acaaggatct tatggacagc ggcgtcgacg tcctctatga cgaccgcgat
     2821 ctgcgagccg gcgagaaatt cgcagactcc gaccttctcg gcatcccgtg gaggctcgta
     2881 gtgagcgcgc gcaacatggc ggcaggaacg ctcgaactca aggaaaggaa gacgggtact
     2941 acggaaagcg tgaacgaatc ggaaattctt aacagctcgg ttatggagaa atttaaggcg
     3001 aactagaaat ggctgacaag ggaatcaagg gaataaagag gctgctttcg aacgatatcg
     3061 gcatcgatct cggcaccgcg aacacgctcg tatacgtgcg cgggcagggg atcgtcctga
     3121 acgagccgtc ggtcgtcgcg gtgaacacga agagcggccg cgtcgttgcg gtcggcgcgc
     3181 aggcgaagca gatgctcggc cgcacgccgg ggcatatcac cgccgtctcc ccgctggtcg
     3241 acggcgtgat ctccgatttc gaagtgacgg aggaaatggt atcgtatctt ctcaatcgcg
     3301 cgcagtcgtc gagcaagaag cttctcggcc cgcgcgtagt ggtgggggtt ccttcgggca
     3361 tcaccaacgt cgagatccgg gccgtgcgcg acgcgacgcg gaacgcgggc gcgcgcgagg
     3421 ttcatatcat cgaggagccg atggcggccg ccatcggtat gcggcttccg gtgaaagacc
     3481 cggtcggcac catggtgatc gacatcggcg gcggcacaac cgacatcgcg gtcatttcct
     3541 tgggcggcgt cgtccgctcg aagagcctcc gcatcgcggg caacaggctg aactccgaca
     3601 tcatttccta cataaggagc gaattcaaga tactcatcgg cgaaaagacc gccgaaaacg
     3661 tgaagatcgc gatcggctcg gtactgcccg gcgaccagcc gatggaagcg gcgatcaagg
     3721 gccgcgacct ggtgaccggc ctgccgcgcg aagtggttat caccgattcc gacgtccggg
     3781 aggcgactcg ccagtcgatc gatacgctgg tcgaatcaat ccgcgaagta atagagacga
     3841 cgccgccgga aattctcgcc gacgtcatgg ttcgcggcgt ctacgtcgcg ggcggcggcg
     3901 cgctcatcaa gggcctcgcc gctctcattg aagacatgat aaagattccg gtgtacaccg
     3961 ccgacgatcc gctcactgcg gtcgcgcgcg gcacgggcgt cgtgctcgag gatatcggag
     4021 cgcacgctga cgtgctcatt gccgacgaaa atgaattacc gcctaaggga tagcagtaca
     4081 aagaaaaaat cccggaaagt ccctctcatc atcctggccg ccgtcgttct cgccctcgtc
     4141 gcgacaagag tattcgcgcc ggcattttcc tcgcgcgcga tcgccgcgat tttcgcgcct
     4201 atcatgagga gcggggatac cttggcgcgc gatgccgccg cttcgggcga cgcgctgaag
     4261 agcaagagcg atctcattgc cgagaacgcg gaattgaaga gcgagcttca ggcggcgcag
     4321 gacgagctcg ccgtctcgcg cgatcttgaa acggaaaacg gcgagcttcg cgacctgctc
     4381 ggcctcgtgc gcgccaccaa ggaaacgccg gcgcttgttc tctccaagcc gccgctctct
     4441 ccctacgatt cgctggtgat cgagatcggc agccagtatc aggtagcggc gggcgaccgc
     4501 gtgtatgccg acggggacat tcttctcgga gaagtggccg aagctgacgg tccgaccgcg
     4561 aaggtgaggc tctattcgaa cgccggcacg gaaatcgatg cggtgatcga gcgcaccggg
     4621 gtgccgcaga aagtcgtcgg cgcaggagga ggcaatttca gcatcaaggc gccgaaggaa
     4681 gtcgacgtga aagcgggaga cgtgctcgtc gttccttctc tcggcaggaa agtcctcggc
     4741 gtggtgaagc aggtcgactc agagcccacc gacgcgttcc agcaggttct catcaaaagc
     4801 cccgtcaata tcttccagct taagtgggtg acgatcgaaa gcggcgatac gccgtaaccc
     4861 cggtcgcatt ccgaaaccga tgacacgagc agcttcaagg atcatactcg acggattcat
     4921 tcttgcgtcg ctcctgtatt tgccgtggtg gatcacggcg ctcgcggcgg cggcgggatg
     4981 ctttctcttc gaatctttct tcgaggcggt cgcattcggc ttcatcgccg actgcctcta
     5041 cggcgtgccg ggccgcttcg gcggcacgac gttcgaagga acgcttgccg ccagcgtcat
     5101 tctcggcctt tcgctgtatc tccggcctca tctccggtac tataagtaaa atttttaatt
     5161 tccaattctt aattttcaat caaatcgcaa tggatcaatt cctgcctgcc ggcaggtcca
     5221 aacgtttgaa tcattggaaa ttgaaaattc attgaaaatt tgaaattgat aattagaaat
     5281 tctttatttc catgctcgaa cgtttcagct caaaagtgcg gaaaatattc aggcgaaggt
     5341 cgcgctatga gggggcggac atcgcacccg acgagatctt cctcgattcg tccaacctgc
     5401 cggacttcga catgagccaa ttcgagggaa ggttcgagaa gccgatcgcc acccgcgccc
     5461 ttcggctcct cggcttcttt ttcgtgctgc tcgcgctcgc atacgcttcg aggctctggt
     5521 tccttcaaat caggagcggc gatctctacg cgaaaatgag cgaggagaac agcgacaaga
     5581 gcaccgtcct cttcgcgtcg cgcggcatca tctacgaccg caacggcgtc gagctcgcgt
     5641 ggaacgacct caatcccgac gacgtgtttc cgctccgcaa atatacgacg gatcccggct
     5701 tcgcgcatgt cctcggatac gtcacgtacc cgggcaagga caagcagggg aatttctatg
     5761 aagacaccta tcagccggtg ggaggcatcg agaagagcga ggatgggacg cttcgcggca
     5821 cgaacggcga gcaaatcgtc gaagaggatg cgctcggcga ggatcaggga ggcgccaaaa
     5881 ctgacgagcc gagggacggc ggcaacgtga agctttcgat cgattcaaag gtggagagcg
     5941 aggtttacaa cgcaatcgcc caaacggcgc acgactacgg gttcactggc ggcgcggcca
     6001 ttatcatgga cgtccacaca ggcgagattc tcgcgtacac gagcttcccc gaattcgacc
     6061 tgaataccat gacgaacggc acgaacgagg agaaggtagc gcagatacag gcgcccgaga
     6121 cgccattcct cgaccgcgtt tcctccggtc tctacacgcc gggctccatc gtgaagccat
     6181 atatgtcgat ggcggcgctc accgagcaca ccatcgatcc gaacaagatc atttatacga
     6241 ccggttcgct caagattccg aatccgtaca atccgggcca gtacagcgtc ttcaacgact
     6301 ggaaaaatct cggtccactc gacatgcggc acgccatcgc gatgtcatcc gacgtttact
     6361 tctacgaagt cggcggcggc ttcggcgacc agaagggcct cggcatcgcg aacatcgaca
     6421 aatacatggc gatgttcggc ttcggccaga aaacaggctt ccaattctcc aacgagaagg
     6481 agggcgtcgt gccgtcgatc gaatggaaga agaagacgtt cggcgaggac tggctcctgg
     6541 gcgacacgta tcactcgtcg atcgggcaat acggctggct ggtaacgccg cttcaggtgg
     6601 tgcgtgcggt cgcgtcggtc gcgaacggcg ggacgctcca tactccgacg cttctcttga
     6661 acgatcctaa tgaggcggcg aacgacaagc atctcgacct gaatcccgac tacttcgaga
     6721 ttgtccgcga aggcatgcgc cttggcgtcc aagaaggagt tgcgaccgcg ctcaatactc
     6781 ccgcggtgaa gatcgccgcg aagaccggaa ccgccgagct cggcattacc aaggactacg
     6841 tcaactcgtg ggtgaccggc ttctttccct atgacaatcc gcactatgcg ttcgccatcg
     6901 tcatggagca tggctcccgc tacaatctca tcggcgcgtc gtttgttatg cggcagatta
     6961 ttgactggat ggccgctaac actcctcagt atttgcaata gaggcttcca tatttggcgc
     7021 gcttctgcgt tggccgctcc gttcgctccc gcagagtacg agcatgtaca ctttggtcgc
     7081 gatactcgcg gccgccttga atcgcatcca aatctgaaag tctttggatg gtggtaactt
     7141 gagaaaaatt tgaaaaagaa aaagcccgcc atatttcaga cgggctttca gaaaaaatgc
     7201 ctattcaatc ttaatgagaa gttgatccag atctgcagcc ttcacagatg acttttcctt
     7261 ctgcgatctc tcgacccagc agataaaaat tacatttaac gcatcttggc tctttggaag
     7321 ggaaccaaag gagtttgagg tatgttgctg ccatgatggc atatgccgcg gcaagtccgt
     7381 aaagatcgtg catgaattag cctcaccctc tgctacatta cattatttat aaaaatgtca
     7441 attttttcaa aaaccttatt aaatatggtt tttctgacag ctgaaagcta aaagctgacg
     7501 gtttttctct caaattgaca tttcgtgggt ctgctataca atagatggac aataaattga
     7561 aatggtcatt atatcccgca atttgcggcg atattttaaa ataaagtaat caatagaatt
     7621 tgctgatttg gatgcaatcc gcgaagccgg cgagtattgc gaccaagctg tacatgctag
     7681 tactgcgcgg gagcaaacgg agccggcgac aaaggagtgc gccaaatatg cgaattctaa
     7741 ctgttatgcc tgacaatcaa aaagaatatc taacccagga aaagttcaat gagctcgaaa
     7801 aggagcttga acagctgaag accgtccgcc gcaaggaagt ggcggaggag ctcgagtatg
     7861 ccaagggcct cggcgacctt tcggagaacg cggaatatca tgaggcgcgc gaccgccagg
     7921 cgactgtcga ggaccgcatc gcgaagctcg aagacatcct gaaacgcgcg gtcatcgtga
     7981 gcgaaaagga ccactcgtcc gacatcgtga acatcggctc gaccgttacc gtgaaaaagg
     8041 atggcgcggg aagcggctta acctataaga tcgtcggcag cgaggagtct gacatgagcc
     8101 aggggcacat ctccatccgc tctcccttcg gcgaatcgat tctcggcaag aagaagggcg
     8161 acgagttcag cttcaaggct ccgagcggaa tgctgactta taaagtcgtt gagatcaagt
     8221 aaaactcagt agtcagtaat gggtagtgag tagaaagacc ggaaccggcg cccaagggcg
     8281 ccggttcttt gtatttttat cccgctactc tatactaact actatctact aagaaccatg
     8341 ttttgggccg ataaaatcgc ggagaaaata gctctgcagt ataaggacaa gatcgcggac
     8401 gggaaaaaac tcatcattcg cgacgagaag acgatgtcgg gccgcgttca cattggctcg
     8461 atgcgcggcg tcgccattca cggcatcgta tcggaagctt tgaaagagcg ggggatcgag
     8521 aacgaattct ttttcgagat caacgacttc gacgccatgg acggtattcc cgtctatctc
     8581 gacagggaaa cgtacgagcc atatatgggc gtttcgctct gcgacatccc gtcgccagac
     8641 ctgactaccg gacaggcagg cggcacggcg aaaaacttcg cggagtattt cgcggaggaa
     8701 tttctcggcg tcatcaagga agcgggcttc gcccccacgt actaccgctc gagcgagaaa
     8761 taccggagcg gcgcgtataa cgatatgatc aagaccgcgc tcgaccgcgc cgaacttatc
     8821 cgcacgatct acaaggaaac ttcgggctcg gtgaagggtg aagactggtg gcctttgaac
     8881 gtccgctgcg agaaatgcgg caagatttcg actactcgcg tcctttcgtg ggacggatcg
     8941 gaagtcgagt acgaatgcag ggacatcgaa tgggccaaag gctgtggaat caggggcaga
     9001 atttctccct tgaacggcaa tgcgaaattg ccgtggaaag tcgaatgggc ggcgaagttt
     9061 agcgtgatcg acgtcgatat cgagggaggc ggcaaggatc attcgacccg cggcggctcg
     9121 cgcgacgttg ccaaccatat ttcccgcgaa gtgttcggcc ggaagcctcc gtttgatatt
     9181 ccatacgaat tcttcctcat cggcggcaag aagatgtcct cgtccaaagg acagggctcc
     9241 tcggcgcgcg aattcgccga tctcctcccg ccccagctcc tgcgctactc gcttctctat
     9301 aaagatatca atcagcagat caatgtcgat ccggaaggcg atgccatccc gcttctcttc
     9361 gacggctacg acagggcggc aggagctttt tggagcggca aggatgacga cgtggcgagg
     9421 ctctttaagc tcgtgaatca aaccgatccg aaactccttg aaaagcgcgc gcttccgcgt
     9481 ttttcaaccg tcgcgtatct cgtgcagatg ccgcatatcg atattgtcgc ggaattcgag
     9541 aagcttgagg ggctctcgta cggcgagcgc gacagggaag agctcgagga gcgggcgaag
     9601 tatgcccgca tctggctcga tacatatgcg cctgaggact tcaagttcga aatcaaggaa
     9661 acgcttcccg cagaggcgaa gaatttcaca cccgagcaga aagccgctct caaaaaagtt
     9721 ttggaatacg taaaatcgca ggctgcattg gacggccagg agcttcacac ggagctccat
     9781 gagatccgca aagcatcagg cctcgaagcg gcgcaattct tcggcgcgct ctacctgtgc
     9841 attctcggaa agcagtcggg gccgaaggcc ggctggttcc tctccgtgct cgacaggaat
     9901 tttctggaaa cgcggctcga ggaagcggca agctagtttg cgtgaatgct gaaattatga
     9961 tattctgctg aaagattttt ctagtttgtt cgcttgagcg attttcctga aatggcaata
    10021 tgggtctcac cgttccctca ttgtttaccc tcctctcaaa accgctcttc tcgcttgacc
    10081 aagtaaaaag caaccaccgc ttcggcggtg gttttcctat tttctaaaat ggagcactga
    10141 tcaggctggg aaggcaaatc cgccttcaaa aagccggcaa ttgcggatca ttattgccga
    10201 gccgtccaat aatgttgagt tctgtggata agagtggtat tgtcgagcta tctggtggta
    10261 tataatgtca attaagtggg ataaagtggt aaatttttag ggagcagctg cgaccataaa
    10321 aattaccacc cctcaccact tccgaccgtt atgcgccggt attcactgag taaacatgag
    10381 catgcgcgct gcgcgccatc tttcgaaaga tccattcgga agcggtgcgg agtgaagatt
    10441 ttctaaagct cgtttactcg ctaagaaaat cattcatgtt tatcggagaa tacacgc
//
LOCUS       DASZYS010000061         6400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_576698, whole genome shotgun sequence.
ACCESSION   DASZYS010000061 DASZYS010000000
VERSION     DASZYS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576698"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..777
                     /locus_tag="VHF05_03590"
     CDS             274..777
                     /locus_tag="VHF05_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80041.1"
                     /translation="MFKHAIDDLKGGSPSRQSLLVINHVAFAQTAFVMSRLGEGNFGT
                     GFNPLPYTTQARIKVSRLQQSGAQSQVIRDEAKKRIYALIGEHQMLDKIFQLQSRSEN
                     IRLFRRPVKFTIYSDSIAGWGPRCNLQFGENTKMVVAASHAEGNNVFYGSLRWNPHFY
                     YLRKRRK"
     gene            complement(845..3010)
                     /gene="recG"
                     /locus_tag="VHF05_03595"
     CDS             complement(845..3010)
                     /gene="recG"
                     /locus_tag="VHF05_03595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVS80042.1"
                     /translation="MTGLTSETPVEIAFRLDLEHKKALHRLHIRTVRDLLYHFPTRYG
                     DIAEVRNISSAVPGEDSVFFGKLSKLEAKKGWKSKIPMASASVSDETGKIDLVWFNQP
                     YLAKMFFEGQLVRIEGKVTERKGKLTISNPKIEKTDKVPVAVGASLFGAEGESHTLYP
                     VYPETKGITSNWIYHHVAKAIKSGVLDELADPLPEEILKKYHLPSLKAALIWIHTPMK
                     MTDAEAARKRFGFEEMFAIHLEKQKEKLLWQSAEAFVINPSQEKMDEFVSGFAFEPTF
                     AQEKAIRTILADFRKGYPMSRLLEGDVGSGKTAVAAATAYAAVVSRPNGQDYGTLQVA
                     YMAPTEILAKQHFESFIQYFAHMGVNIGLITGSGCRKFPSKVNPKTWTDISRNQLLKW
                     VANGEIAILVGTHALIQKSVKFKHLAYVVIDEQHRFGTAQRQKLVTKEGKTPHLLSMT
                     ATPIPRTLALTMYGDLDLSLLDQMPQGRKPIITEIVTPDRRDATYEEIRGRLKEGRQL
                     YVICPRIDEPDPLKALALNTKSVKEEAKRLKKSVYPECEIAILHSKMTPREKEKTMEA
                     FASGEIDILVATSVVEVGVNVPNATMIIIEGGERFGLSQLHQLRGRVLRGTHQAYCYV
                     FADSKSDRTYERLKAFKTAANGFELAEFDLAQRGAGELYGGKQWGVSDIAMEGIKNLK
                     LVEASREEAAALIERDPDLLRHPLLKKRLEEEAKKIHFE"
     gene            complement(3322..4182)
                     /locus_tag="VHF05_03600"
     CDS             complement(3322..4182)
                     /locus_tag="VHF05_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVS80043.1"
                     /translation="MLTNMERVKEFKKIVWSHYRRSGRDLPWRRTRDPYRIFVSEIML
                     QQTQVPRVIPKYESFIRKFPSFKALAAASLRDVLAEWKGLGYNRRGLNLKRAAEMVVK
                     DFRGKLPKTAEELVKLPGVGPNTAGSLLAFAHNLPLPFIETNIRSAYIHFFFQGKTNI
                     HDRDILPLIRKTLPKQSARLPRSNSRPGEFTIGEWYYALMDYGSWLKREHPNPSRRSA
                     HHARQSPFKGSHRELRSKVLQYVLENSRATAKKAAKTLRLEEAETADILRELAAEGLI
                     KGNRRSFIVE"
     gene            4249..5445
                     /locus_tag="VHF05_03605"
     CDS             4249..5445
                     /locus_tag="VHF05_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVS80044.1"
                     /translation="MKKLQIVKKYVNVTDVKVLTNIRFYTVAGIAQYLSNFIRYNERT
                     SEASIDIYGVDIMLPGEEQNLRTTEIASMKHFHLLARTIAYPSLKDVVHTAKSLDDVR
                     TAYASVTDAYYEAIEKVRPDVILVNGTFFLPWCLLQAALRYGKARIVLHYHGVLTKEV
                     EHWKEEDAKQLFHKMEREFDRPEVSYVFPSRHAKRTVEREVYNHPVRRSIVLPNPVSK
                     SFFRAPKRRKNPVIGMVSRWTKVKNPSFLISLARYSKKTDSGYEIHAITDIKPHMPDY
                     RKVLSLITLFSPVENHRLPAFYRNLGVLILPSHFETYGNVAQEALATGVPVLVSSKAG
                     VAETLKNVGLSDWVIDFSSPEKVLEKVEEVIDAGVPEKSLKRLRADYTNETIYSKYAR
                     FLRDEQ"
     gene            5546..6082
                     /locus_tag="VHF05_03610"
     CDS             5546..6082
                     /locus_tag="VHF05_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVS80045.1"
                     /translation="MTREEYGAAYEKGLVPTERFLIARFKLTLDEARDFAQAAWVRGW
                     EMRAQYRQEGAVQTWVFTIARNIVNTSYRKKREMVDFESLNLTVAAHNPEPKMTVGRL
                     LSQLGWDERILLILKYREGMSLEEIMETTGLPPTTAKTRLHRARLKLQNILRNTGERK
                     GEKRKCLARQMQEIWENA"
     gene            6244..6332
                     /locus_tag="VHF05_03615"
     tRNA            6244..6332
                     /locus_tag="VHF05_03615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6278..6280,aa:Ser,seq:gct)
BASE COUNT         1422 a         1579 c         1808 g         1591 t
ORIGIN      
        1 tataagacgg ctgctctacc aactgagcta tacgcccatt tttatttgtc tctcgctgcc
       61 atctgtaagc tcggtgctct aaccaactga gctacacacg catggcttta agctgcaaaa
      121 atcttacccc aaagccctcg aaggggcaac gaatggggat aggataattt gacactattt
      181 tttaaatatt gcaaaattaa agaaggaggg aatgtgagac tgcttgtact tttcatatgc
      241 tttgcgttcc tacccgtctt cgggcagaac gcgatgttca aacacgcaat cgacgacttg
      301 aagggcgggt cgccttcgag gcaatcgctt ttggtaatca atcatgtggc attcgcccag
      361 accgcgttcg tcatgagccg gctcggagaa ggcaatttcg gaacgggttt caatccgctt
      421 ccgtacacga cccaggccag gatcaaagtg tctcggctgc agcaaagcgg agcgcagagc
      481 caggtaatac gggacgaagc caaaaagcgc atctacgcgc tcatcggcga gcaccaaatg
      541 ctggataaaa ttttccagct gcaaagcaga agcgagaata tacggctctt ccgccgtcca
      601 gtaaaattta caatttactc ggattcgata gccgggtggg ggccgcgttg caaccttcag
      661 ttcggcgaaa atacaaaaat ggttgtagcc gcatcgcatg cggaaggcaa taatgtcttt
      721 tacggcagtt tgaggtggaa cccgcatttc tattacctgc ggaaacggag gaaataggga
      781 atgcgagccc cgccaaagcg cgaattattt ataattcgcg ctttcttttt ttgatttctc
      841 cgctttactc gaagtgaatt ttcttcgcct cttcttcgag gcgcttcttg agaaggggat
      901 ggcggaggag gtccgggtcg cgctcgatga gcgccgccgc ttcctcgcgc gaggcttcga
      961 cgagcttcaa gttcttgatg ccttccatgg cgatgtcgga gacgccccat tgcttgcctc
     1021 cgtagagttc tcccgcgccg cgctgggcaa ggtcgaactc ggcgagctcg aagccgttcg
     1081 ccgcagtctt gaaggccttg agccgctcgt acgtgcggtc gcttttcgag tcggcgaaga
     1141 cgtagcagta tgcctggtga gtgccgcgga ggacgcggcc gcggagctgg tggagctggg
     1201 agaggccgaa gcgctcgccg ccttcgatga ttatcatcgt cgcgtttggg acgtttacgc
     1261 ccacttcgac aacggacgtt gcgacaagaa tatcaatctc gcccgaggcg aacgcttcca
     1321 tcgttttttc tttttcgcgc ggcgtcatct tgctgtggag gatcgcgatc tcgcattctg
     1381 ggtatacgga ttttttgagc cgcttcgctt cttctttcac cgacttggtg ttcaaggcaa
     1441 gggctttgag tggatcgggc tcgtcgatgc gcgggcagat gacatagagc tggcggcctt
     1501 ccttgagccg cccgcggatt tcctcgtacg tggcatcgcg gcggtcaggc gtgacgattt
     1561 ccgtaatgat cggcttgcgg ccctggggca tctggtcgag aagcgagaga tcgagatcgc
     1621 cgtacatcgt gagagcgagc gttctcggta tcggcgtcgc ggtcatcgag aggaggtggg
     1681 gagtcttgcc ttcctttgtt acgagcttct gccgctgggc cgtgccgaag cggtgctgtt
     1741 cgtcgatcac cacatatgcg agatgcttga atttcaccga tttctgaatg agggcgtggg
     1801 tgccgacaag gatcgcgatc tcgccgttcg ccacccattt gaggagctgg tttcgggaaa
     1861 tgtccgtcca tgtcttgggg ttcaccttcg aggggaattt cctgcagccg gagccggtaa
     1921 tgaggccgat attcactccc atgtgcgcga agtactgaat gaacgattcg aagtgctgct
     1981 tggcgaggat ttcggtcggc gccatatagg cgacttggag cgtgccatag tcttgtccgt
     2041 tcgggcgcga gacgactgcc gcgtacgcgg tcgccgccgc gactgccgtc ttgccggagc
     2101 cgacgtcgcc ttcgagaagc cgtgacatcg ggtagccttt tctgaagtcg gcgagaattg
     2161 tgcggatcgc cttttcctgc gcgaaggtcg gctcgaaagc gaagccggaa acgaattcgt
     2221 ccattttctc ctgcgaagga ttgatgacga aagcctcggc gctctgccag agcagtttct
     2281 ccttctgctt ctcgagatgg atcgcgaaca tttcctcgaa gccgaagcgc ttgcgggccg
     2341 cctcagcgtc ggtcattttc atcggcgtat ggatccagat gagcgcggct ttaagggatg
     2401 gcagatggta tttcttgaga atttcctccg gcaggggatc ggcgagttcg tcgaggacgc
     2461 cgcttttgat cgctttcgcg acgtggtgat aaatccagtt cgaggtgatg ccttttgttt
     2521 ccggatagac gggatagagc gtatgcgact cgccctcggc tccgaagagc gacgcgccga
     2581 cggcgaccgg caccttgtcg gtcttctcga ttttcgggtt cgatattgtg agcttccctt
     2641 tgcgctcggt gactttgcct tcgatgcgca cgagctggcc ttcgaagaac atcttcgcga
     2701 gatagggctg gttgaaccac acgagatcga tctttcccgt ttcgtcggaa acggaagcgg
     2761 acgccatggg gattttgctt ttccatcctt tcttcgcttc cagcttcgag agcttgccga
     2821 agaagacgct gtcttctccc ggaaccgcgc ttgagatatt ccgcacttcg gcgatgtcgc
     2881 cgtagcgggt cgggaagtgg tagaggagat cgcggacggt tcgtatatgc aggcggtgga
     2941 gcgctttctt atgctcgagg tcgaggcgaa atgctatttc cacgggagtc tcggaagtga
     3001 ggccggtcat gggcatattg tagcagaagg catttgtggg agagcttatt gacttatata
     3061 aaaaattttt atctaatatt tacagaggta ccgaaacatg acacgcgaaa taaccgccat
     3121 agcttttggc gtgccggttg cagcggttgc cattgcgctt tgcatgctgt acggacttgc
     3181 cgatagtccg acaaccgtca tcggcatagc ggcattcctg ccggtgtatt ggtcgactca
     3241 ttttataatg cggatgcgct ctcgcccgca gaaacaagaa gacgaacttt aggacaagca
     3301 gcggagatcc cgctgctttt gctattccac gataaatgaa cggcggtttc ccttgataag
     3361 gccttctgcg gcgagttcgc ggagtatgtc cgccgtttca gcttcctcga gcctcaaggt
     3421 ttttgccgct tttttcgcgg ttgcgcgaga gttttcgagg acgtactgca ggaccttcga
     3481 gcgcaattcg cgatgcgagc ccttgaaagg cgactgccgc gcgtgatggg cgctccggcg
     3541 gctcgggttg ggatgctcgc gcttcagcca cgagccgtag tccataaggg cgtagtacca
     3601 ctccccgata gtaaattcgc cagggcgcga attactacgg ggcaggcgcg cggattgctt
     3661 cggtaaggtt ttcctgatga gcggcaggat gtcccgatcg tggatgttgg tcttaccttg
     3721 gaagaagaag tgaatgtaag cgctccggat gttcgtttcg ataaacggga gggggagatt
     3781 gtgggcgaag gcaagaagcg acccagcagt attcggcccg acgccgggca acttcacgag
     3841 ttcctcggct gttttcggaa gtttgccgcg aaaatctttc acgaccatct cggccgcgcg
     3901 cttgagattg aggcctcggc ggttatagcc taggcctttc cactcggcga gcacgtcgcg
     3961 gaggctggcc gcggcgagcg ccttgaatga gggaaatttc ctgatgaagc tttcgtattt
     4021 gggaataacg cgcggcactt gggtctgctg aagcatgatt tcggaaacga aaatccggta
     4081 cggatcgcgc gtcctccgcc acggcaggtc gcggccgctc cttcgatagt gactccatac
     4141 gattttcttg aattctttca ccctctccat attcgtgagc acattatcgt gcaatttcgc
     4201 agtttattca aattaaaatt cattcttttc atctgaccat atgaatgaat gaaaaaattg
     4261 caaattgtaa aaaaatatgt taatgttact gatgtgaaag tgcttacgaa tatacggttt
     4321 tacaccgtag cgggtattgc ccagtatctt tcgaacttta tccgctataa cgaacgtacg
     4381 tccgaagcat caatcgatat ctacggcgta gatatcatgt tgccggggga agagcagaat
     4441 ctccgcacca ccgaaattgc ttcaatgaag cattttcatc tccttgctcg tactatcgcg
     4501 tatccttccc tcaaagacgt agtgcatacc gcgaagtcgc tcgacgacgt tcgcaccgcc
     4561 tatgcttctg tgacggacgc atattatgaa gcgatcgaaa aagtaaggcc tgatgtgata
     4621 ctcgtcaatg gcaccttctt tctgccgtgg tgccttcttc aagccgcgct tcgttatggc
     4681 aaggcacgca tcgtgctcca ttatcacggc gtgcttacga aagaagttga gcattggaag
     4741 gaagaggatg cgaaacaact tttccacaaa atggagaggg agttcgaccg gccggaagtt
     4801 tcatacgtct ttccctcgcg gcacgcgaag cgcaccgtcg agcgcgaagt gtacaatcac
     4861 ccggtgcgcc gttcgatcgt tcttccgaat ccggtatcga aatcgttttt ccgcgcgccg
     4921 aagcgcagga agaatccggt aatcggcatg gtaagccgct ggacgaaggt aaaaaatcct
     4981 tcgttcctca tctcgcttgc gcgttacagc aagaagaccg acagcggtta tgaaattcac
     5041 gccattaccg atatcaagcc tcacatgccg gattaccgaa aggtgttgtc gctcatcact
     5101 ctattctcgc ctgttgagaa tcatcgtctg cctgcgttct atcgcaatct cggcgtgctt
     5161 attctgccgt ctcatttcga aacatatggc aatgtggcgc aggaagcact tgcgactggc
     5221 gtccctgttc ttgtgagttc caaggccggc gttgcagaaa cgcttaagaa tgtgggacta
     5281 agtgattggg tcatcgactt ctcctctcct gaaaaagtac ttgaaaaagt cgaggaggtg
     5341 atagatgccg gcgttccgga aaaatctctt aaacgtcttc gggccgatta tacgaacgaa
     5401 acaatttatt ccaagtatgc tcgattcctg cgcgacgagc aataaattca atgcccactg
     5461 aggttgatct tcacagatga tcttttttgt gatattttga ttttagtagg aatcaaaatt
     5521 tttgtttttt ggaaaaggag gctgtatgac gcgagaggaa tacggtgcgg catatgaaaa
     5581 agggcttgtg ccgactgaaa gatttcttat tgcacgtttc aagcttacgc tcgatgaggc
     5641 tcgggatttc gcccaggctg catgggttcg cgggtgggaa atgcgcgcac aatacagaca
     5701 ggaaggcgct gtccagacct gggtattcac cattgctcgc aacattgtga atacaagtta
     5761 tcggaagaaa agagagatgg tcgatttcga atcgttaaat ctcactgttg ctgctcataa
     5821 tccggagcca aaaatgaccg tggggagact attgagccaa cttgggtggg acgaacgcat
     5881 cctcttgata cttaagtata gggaggggat gagcctagaa gaaatcatgg aaaccacagg
     5941 gcttccgcct actactgcaa aaacccgact acacagagca agactgaaac ttcagaatat
     6001 tttgcgcaat acgggggaga gaaaaggtga aaagaggaag tgtctcgcaa ggcaaatgca
     6061 ggagatttgg gaaaatgcat gactccagcg cagtgcatat tgatatgcac tgcgctttct
     6121 ttgtgcctag aagctcacgg ggaagtatga taaggtagta gcgacaagat gtggaaagat
     6181 ggctaaaaca ctcggctggg agccggttgt tcgtccgaac attttgtcta caaaatgttt
     6241 tagggagagt tggctgagtg gtcgaaagcg cctcactgct aacgaggtag gggtctaaaa
     6301 gcctctcgtg ggttcgaatc ccacactctc cgcagacggc cgaaaaagac agctgggagc
     6361 tgtctttttc gtttgccgct gcgcgagagt gtgggattcg
//
LOCUS       DASZYS010000062         7013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_126370, whole genome shotgun sequence.
ACCESSION   DASZYS010000062 DASZYS010000000
VERSION     DASZYS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126370"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(444..1574)
                     /locus_tag="VHF05_03620"
     CDS             complement(444..1574)
                     /locus_tag="VHF05_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HVS80046.1"
                     /translation="MQAEDTKEVNGLEHEEGDEKAAKTKKDTIMTKFVADSPNPPSVE
                     DLVEGPVISIDKSAIYIDLSPFGTGIIFGREFMSARDIVRKLNIGDNIAGKIVSIDNK
                     DGYYEISIKEARQALIWSEAEEAIKDKRVYELTVQEANKGGLLLMWQGIQGFLPASQL
                     KTEHYPRVADGDKDKILEELRKMVGQKITVSIIAVVPKEGKLIFSEKSPQEKNKEQII
                     GKYSLGDIVEGEVTGIVDFGVFVKLEEGLEGLVHISEIDWALVENPRALYKVGDKVKV
                     KIIEIKDGKISLSIKALKENPWVEAAGKYKKDQEVSAVVIKFNKHGALASVEEGIAGL
                     VHISEFGSEEKLREKLELGKSYKFKITLFEPKEQKMTLSYIG"
     gene            2440..3075
                     /locus_tag="VHF05_03625"
     CDS             2440..3075
                     /locus_tag="VHF05_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVS80047.1"
                     /translation="MIITYGGLEAFKLQFGDTVISLNPASKDSKFKTPSYGADIALVS
                     LNHEDMNGLETVSRGDRRPFEISGPGEYEVGGIFIKGMQSKSRYGGEEKWNTIYTIGL
                     EGMNLCFLGALGDAESAKEARQNIDDVDILFVPIGGDGVLDPAAAYKFALSLEPRIMI
                     PMHYGDVGAKDALKVFLKEGGSEKVETQDKLTVKRKDLDGKQGEIVVLGAS"
     gene            3122..3538
                     /locus_tag="VHF05_03630"
     CDS             3122..3538
                     /locus_tag="VHF05_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80048.1"
                     /translation="MGIKRHIEDLRKEPPHVQRRYSLLYSGLVTGAIFIVWLSVLLPQ
                     GLRHDAAEASKSAAGENLAAVGSALQNGYDNAAQDMEAASAGNSDNSDISGDSANSYS
                     AGTGLMGSLPPQTAADATDSAADSASAPQKQYDFGY"
     gene            3645..6113
                     /gene="gyrA"
                     /locus_tag="VHF05_03635"
     CDS             3645..6113
                     /gene="gyrA"
                     /locus_tag="VHF05_03635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVS80049.1"
                     /translation="MAKKTSEQPEMNDFQERRSIIPAPITTEMKDSFIDYAMSVITER
                     ALPDVRDGLKPVHRRILFSMNEKGLTATAKTRKSATVVGDVIGSYHPHGDVAVYDAMV
                     KMAQDFAMRYPMIIGQGNFGSIDGDPPAAYRYTEAKMSRMAAEMLRDIDSDTVDFMPN
                     YDGTKKEPVVLPAAVPNLLLNGTLGIAVGMASNIPPHNLGELVDASTHLIDNPEATTE
                     DLLEFVKGPDFPTGGIAFDQREIQHAYATGRGGVVTRGIADIVEDKKGNPQIVITGIP
                     YRVNKASLIEKIAELVRDKKIEGVKGLRDESTKDIRVVIDLKGGSHPQKVLNALYKHT
                     QLEETFHYNLVALVDGVPQTLSLKGILQEFVKHRFIVVKRRTEFDLRKAEAREHILLG
                     LKKALDHIDAIIKLIRASKDTPTAHANLMKEFKFSDLQATAILDMKLQRLAGLERKKI
                     EDELKEVQALIKELQDILSSSKKIFAVVKKEMNEVREKYGDERKTRVIKGGVKVISVE
                     DLIPDEENALVLTAGGYIKRTNPDEFRKQKRGGVGVVDLETKEEDFVTNLLIASTHSD
                     LLFFTDRGKAYQIKMYDIPEGRRATRGKSVMNFLSLEQGERITSILPMPKEVKASKLS
                     IMMVTKQGVAKRVDAASFHDVRRSGIISIRLEAGDQLVSAAFTSAGDTTIIVTAKGQS
                     IRFKESDVREMGRTAGGVRGVSLDKGDSVIGAHTVSKDAKNAELLVLASHGYGKKTPL
                     SEYKVQGRGGSGIKTAQVTAKTGEVMASQVVTEEEEEVIAMSKQSQVIRTDLKSIPTL
                     GRQTQGVRVMKLREGDSIASVICL"
     gene            6645..>7013
                     /locus_tag="VHF05_03640"
     CDS             6645..>7013
                     /locus_tag="VHF05_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVS80050.1"
                     /translation="MFADPQKNIEQFGLQPGQKVADFGAGSGFYSFAAAQAVTSKGTV
                     YSIDVQQDLLAKLTNEARRQGISNIETIWGDAERLGATKLADGSMDAVIISNLLFQLE
                     DKPGAAKEALRILKKGGRALV"
BASE COUNT         1672 a         1867 c         1907 g         1567 t
ORIGIN      
        1 tccatccgtc tgattggccc caacggccaa tgtcaacccg attgggtcgc agggtatgac
       61 ttatagttcc gataggatga gcttggaatg ttccttcatt ctgataaaca tacctgaaga
      121 cgggcaggcc tccgacttgg atgaaggcca tgtcttccag cttcttgttc tgcttgcgtt
      181 gagtgtattt gaatacgata tctcgcggca tgtatcctcc tctgtgatac gaaatccagc
      241 tctaaattac cacgtgcacg aatttagtca aaaagaaaac cgcccaacgg gcggttttcc
      301 taatttgaat tactttctta aaaacttgct cagtcggatg caatccgcga aaccggcgag
      361 gcttgcgacc aagctgtaga aatactactg cgcgggagta aacggagccg gtgacaaagg
      421 agtgcgccgt ctgtgcaagt tttttatccg atgtaggaga gggtcatttt ttgctccttc
      481 ggctcaaaga gggtgatttt gaacttatag ctcttgccga gctcgagctt ctcgcggagc
      541 ttctcctcgc tgccgaactc gctgatatga acgagtcccg cgataccttc ctcaaccgag
      601 gcgagggcgc cgtgcttgtt gaacttgata acgaccgccg agacttcctg gtccttcttg
      661 tacttgccgg ccgcttcaac ccacgggttt tccttcaagg ccttgatcga gagggaaatc
      721 ttgccatcct tgatctcgat gatcttcacc ttgaccttgt cgccgacctt gtagagcgcg
      781 cggggattct cgacgagcgc ccagtcgatc tcggaaatgt gcacaagtcc ctcgaggcct
      841 tcttcgagct tcacgaacac gccgaagtcg acgatgccgg taacttcgcc ctccacgata
      901 tcgccgagcg agtacttgcc gatgatctgc tccttgttct tctcctgcgg gctcttctca
      961 gagaagatga gcttgccttc cttcggaacg accgcgatga tcgagacggt gattttctgt
     1021 cctaccatct tgcggagctc ctcgaggatc ttgtccttgt cgccgtcagc gacgcgcgga
     1081 taatgctcgg tcttgagctg ggatgccgga aggaatccct ggatgccctg ccacatgaga
     1141 aggagccctc ccttgttggc ttcctggacg gtgagctcgt agacgcgctt gtccttgatg
     1201 gcttcctccg cttcgctcca gatgagcgcc tggcgggcct ccttgatcga gatctcgtag
     1261 tagccgtcct tgttgtcgat cgaaacgatc ttgccggcga tgttgtcgcc gatattgagc
     1321 ttcctgacga tatcgcgggc gctcatgaac tcgcgtccga agatgatgcc ggtgccgaac
     1381 ggcgagaggt cgatgtagat cgccgacttg tcgatcgaga ttaccgggcc ctcgacgagg
     1441 tcttccactg aaggcggatt cggcgaatca gcgacgaact tcgtcatgat cgtgtctttt
     1501 ttggtcttgg cagctttctc gtcgccttcc tcgtgctcga ggccgttaac ttctttggtg
     1561 tcttctgctt gcatgtaatg aaaagggcct tgcgttcgct ccaagccgtc tcggagcggg
     1621 ttagcgcaaa gcgggtcttt taataatgtg ccgtgcggcc gaagccgcgg cggccaatta
     1681 aatatataga aatcaaggga aaagtcaaaa gaaaagaaaa gaggaagaat gagggagaaa
     1741 taaggaaaat cagacaaaca aaaaaccctg cgttcacata agtgattgca gagtgcgttg
     1801 gatgaaattt ttctcaaaaa tttcgaaaaa cggattggtt tgagatttgt gaaatctcgg
     1861 aaactttggt tggtgaagat tgaaactcaa tcttcaaagg aacaatgtaa gtattaaact
     1921 tacactagta tagtaatccc caccaacaac aaatgcaagt gcgtttgatg gttggagaat
     1981 gaaataaagt ctttgtcaaa agaggctccc tgaaggaagc ctcatgatac gttaattact
     2041 cgttccgccg gttcgcccaa aagtggggaa tcggtagaag aaatagcgtt atttgcatcc
     2101 gggtcgccga ggccgaacct cataacgtta cccgccagaa tggcgaaccc aagcccgccg
     2161 aagagccaca cagtgaaaaa actgatccag aaccacatca taatgttaac tcactccttt
     2221 tttaataata ccatactttt atcgaaaaag tcaatactac aaaagctcat attcgaacgc
     2281 tttttatgct aaaatgcctc catgatcctg tagtagaatt tcaggttgcg aacggtgctg
     2341 ctaaaggatt tggatgatgg atgtttaggc tggtaaatta aattttcgct aatttgggac
     2401 agttcgcatc tggcgaacga cctgaaattt cactacagga tgattatcac atacggaggg
     2461 cttgaagctt tcaaactgca attcggcgat acggtcattt cattgaatcc ggcttccaag
     2521 gattcgaagt ttaagacgcc gtcctatggc gcggatatcg cgctcgtaag cttgaaccac
     2581 gaagatatga acggcttgga aacggtgtcg cgcggcgatc gccggccgtt cgagatttcc
     2641 ggtccgggcg agtacgaagt cggcggcatt ttcatcaagg ggatgcagtc gaagtcgcgc
     2701 tacggcggcg aagagaaatg gaacaccata tatacgatcg gcctcgaggg catgaatctc
     2761 tgcttcctcg gagcgcttgg cgacgcggaa tcggccaagg aggcgcgcca gaatatcgac
     2821 gacgtcgaca ttctcttcgt gccgatcggc ggcgacggcg tgctcgaccc ggcggccgcg
     2881 tacaagttcg cactttcgct cgagcctcgc atcatgatcc cgatgcacta tggcgacgtc
     2941 ggcgcaaagg acgcgctcaa ggtgttcctc aaggagggcg gcagcgagaa agtcgaaacg
     3001 caagacaagc ttacggtgaa gcggaaggat ctggacggga agcagggcga gatcgtcgta
     3061 ctcggcgcat cttgatccgc acgtctttta tttttgattt ttaaccttta atctaaattc
     3121 catgggcatc aagcggcaca tcgaagatct tcgcaaagag cctccgcacg tgcaaaggag
     3181 atactcgctc ctttattcgg gccttgtcac cggcgcgatc ttcatcgtct ggctctcggt
     3241 gcttcttccg cagggcttga ggcacgatgc ggcagaagcg tcgaagtctg cggcagggga
     3301 gaatcttgcg gcagtgggca gcgcgcttca gaacgggtat gataatgccg cgcaggatat
     3361 ggaagccgct tccgccggca attctgacaa ttccgatatt tccggcgact cagccaattc
     3421 ctattcggcg gggaccggcc tcatgggcag cttgccgccc caaacggcgg ccgacgcgac
     3481 tgattccgcc gcggactcag cttccgcccc gcagaagcag tatgatttcg gatactaata
     3541 aacaagcaat tttcaattta aaattttcaa ttttcaaaaa atcagatgtc tgaaaattga
     3601 aacttgaaaa ttcattgaaa attagaaatt gaaaattttt aactatggct aagaaaactt
     3661 ccgaacaacc agaaatgaac gacttccagg agcgcaggtc gatcattccc gccccgatca
     3721 cgacggaaat gaaagattcg ttcatcgact acgcgatgtc ggttatcacc gagcgcgccc
     3781 ttcccgatgt ccgcgacggt ttgaagcctg tgcaccgccg catccttttt tccatgaatg
     3841 agaaaggcct tacggcgacg gccaagaccc gcaagtcggc aaccgtggtc ggagacgtga
     3901 tcggatcgta ccacccccac ggcgacgtgg cggtctatga cgccatggtc aagatggcgc
     3961 aggatttcgc tatgagatac ccgatgatta tcggccaagg aaacttcggt tcgatcgacg
     4021 gcgatccgcc ggcggcatac cgctacaccg aagcgaagat gagccgcatg gccgcggaga
     4081 tgcttcgcga catcgacagc gatacggtcg acttcatgcc gaactacgac ggcaccaaga
     4141 aagagccggt cgtgcttccg gccgccgttc cgaacctgct tctcaacggc acgctcggca
     4201 tcgcggtcgg catggcgtcg aacatcccgc cccacaacct cggcgagctt gtcgacgcgt
     4261 cgacgcatct tatcgacaat cctgaggcga ccaccgaaga tctcctggaa tttgtgaaag
     4321 gccccgattt tccgacgggc ggcatcgcct tcgaccagag ggagatccag catgcgtatg
     4381 cgaccggccg cggcggcgtg gtaacgcgcg gcatcgccga catcgtggaa gacaaaaaag
     4441 gcaatccaca gatcgtcatc accggcatcc cgtaccgcgt caacaaggcg tcgctcatcg
     4501 aaaagatcgc cgagctcgtt cgcgacaaga agatcgaggg cgtgaagggc cttcgcgacg
     4561 aatcgacgaa ggacatccgc gtcgttatcg acctcaaggg cggcagccat ccgcagaaag
     4621 tgttgaacgc cctctataag cacacccagc tcgaagaaac tttccattac aacctcgtgg
     4681 cgctcgtcga cggcgtgccg cagacgcttt ctctcaaggg catcctccag gaattcgtga
     4741 agcatcgctt catcgtggtg aagcgccgca cggaattcga tctccggaaa gccgaagcgc
     4801 gcgagcacat tctcctcggc ctgaagaagg cgctcgatca tatcgacgcg atcatcaagc
     4861 tcatccgcgc ctcgaaggat acgccgaccg cgcacgcgaa tttaatgaag gagttcaagt
     4921 tctccgacct tcaggcgacg gccatcctcg acatgaagct ccagcgtctc gcgggcctcg
     4981 agcgcaagaa aatcgaggac gagcttaagg aagtgcaggc gctcatcaag gaacttcagg
     5041 acatcctctc gagctcgaag aaaatctttg ccgtcgtgaa gaaggaaatg aacgaggttc
     5101 gcgagaagta cggcgacgag cgcaagacgc gcgtcatcaa gggcggcgtg aaggtgattt
     5161 ccgtcgagga cctcattccc gacgaggaga acgcgctcgt gcttacggcc ggcggctaca
     5221 tcaagcgcac caaccccgac gaattccgca agcagaagcg cggcggcgtc ggcgtcgtcg
     5281 accttgagac caaggaggag gatttcgtga cgaaccttct tatcgcttcc acgcactccg
     5341 accttctctt cttcaccgac cgcggcaagg cgtatcagat caaaatgtac gatattccgg
     5401 aaggccgcag agcgaccagg ggcaagtcgg tcatgaattt cctttcgctc gagcagggcg
     5461 agcgcatcac gtcgattttg ccgatgccca aggaagtgaa ggcttccaag ctctcgatca
     5521 tgatggtcac gaagcaggga gtggcgaagc gcgtcgacgc ggcgagcttc cacgatgtga
     5581 ggcggagcgg catcatctcc atccgcctcg aggcgggcga ccagctcgtc tcggcagcgt
     5641 ttacgagcgc gggcgacacg acgatcatcg ttaccgccaa ggggcagtca atccgcttca
     5701 aggaatccga cgtccgcgaa atgggacgca ccgccggcgg cgtgcgcggc gtttcgctcg
     5761 acaagggaga ttccgtgatc ggcgcgcata cggtgtccaa ggatgcgaaa aatgccgagc
     5821 ttctcgtgct tgcctcgcac ggctacggca agaagacccc gctttccgaa tacaaggtgc
     5881 agggcagggg aggcagcggc atcaagaccg cgcaggtgac ggcgaagacc ggagaagtca
     5941 tggcctcgca ggtcgtgacg gaagaagagg aggaagtcat cgccatgtcg aagcagagcc
     6001 aggtcatccg caccgatctg aagagtattc cgacgcttgg aaggcagacg cagggagtga
     6061 gagttatgaa gctacgggaa ggagattcga tcgcatcggt gatttgcctc taaacttcca
     6121 gaattggcgc acttctttgt cactcgtttg cgttcgcttc tcaccgtata tgaatacggt
     6181 tcgggcgcga gctgcactcg tttcgcgaat tgcatccaat tctgaaagtt tttgatccat
     6241 cttaaattta aagaaaaccg ccagaaacgg cggtttttgc tacattgact tttaatataa
     6301 attggtgtat aatggatcca gaaaggtggc aaagagcttg tttaacttct tctccaagat
     6361 ttataactgg ctactcgaga gcttctccga ggttgagcct gaagtattgg cccaagttct
     6421 ggtaaagtcc tatacgcaaa cttttatggg gacggtcatt taataaaaca tgatctcttg
     6481 tccctaaagc tccggctgaa tttattcacc ggagcttttc cttttaggtc tattgtcttt
     6541 atacttttta cctgtgagta gcttccttgt tgtagcgtag cgcctgaaat ataatgaatg
     6601 cataaggaat ttccgcgcct atgagcggcg ggagctttgc atcaatgttt gcagatccgc
     6661 agaaaaatat cgaacagttc ggtttgcagc ctggacagaa ggtcgccgat ttcggcgccg
     6721 gttccggttt ctattcgttt gccgcggcgc aggccgtgac gagcaagggc acggtctatt
     6781 cgatcgatgt ccagcaggat cttctcgcca agctcacgaa cgaagcccgg cggcagggca
     6841 tttcgaacat cgaaacaatc tggggcgacg cggagcgcct cggcgcgacc aagcttgcgg
     6901 acggttcgat ggacgccgtc atcatctcaa acctcctttt ccagcttgaa gacaagccag
     6961 gcgccgccaa ggaagcgctc cgcatcctca agaaaggcgg ccgcgcgctc gtt
//
LOCUS       DASZYS010000063         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_192266, whole genome shotgun sequence.
ACCESSION   DASZYS010000063 DASZYS010000000
VERSION     DASZYS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192266"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..648)
                     /locus_tag="VHF05_03645"
     CDS             complement(25..648)
                     /locus_tag="VHF05_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80051.1"
                     /translation="MAILGDKKYGAAEINFVDYNYGYVSYSLRLLYSGKSLINPELLA
                     EDPFHFDEYENDYLIPFFENLLQEDNESVFKSLEPEVRIDAHFNPKLGLEDALKIEGA
                     WYSDKYKGKLQKVDDDRQKSGGKLQDDGFVFCFYINEHKLRPWREQKSAYGDFVPAML
                     IGVSRQELEDFLNQLKKDYKEWKVRNKEQIEYYGREKSEAKQPEDGN"
     gene            1885..2274
                     /gene="raiA"
                     /locus_tag="VHF05_03650"
     CDS             1885..2274
                     /gene="raiA"
                     /locus_tag="VHF05_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVS80052.1"
                     /translation="MKINTKATNMELTQAINDYVAKMVEAIEKFIDLGDESAILDIEV
                     GKTTNHHRTGDYYRAEVNLEWAGRQYYSAAEKDDLYAAIDEVKDQVVSELAKSKDKHA
                     ERIREGGKEAKEMLHKDPSEEEIEEML"
     gene            complement(2428..3294)
                     /gene="fmt"
                     /locus_tag="VHF05_03655"
     CDS             complement(2428..3294)
                     /gene="fmt"
                     /locus_tag="VHF05_03655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVS80053.1"
                     /translation="MNPRTLNFAFFGTPAFVIPVLEKLKNAGFIPSLVVTAPDKPQGR
                     DHILTPPAVKVWAEENGIPVEQPAKVRGNTDFFTKLKARNFDLFIVAGYGKILPQEIL
                     DLPAKGTLNVHPSLLPLHRGPAPVESQILSGDENVGVSVIALDAEMDHGPIVAQKSFP
                     MPEPLPRAPELDELLWPEGGKLLAEVLPQWIDGSVMPKEQDHSKAAFTKKFAKEDGLI
                     DLADNPLLNFRKFKAHDPWPGTYFFVDQHGKKIRVIVKEADLIDGQFVVRRIIPEGKK
                     EMNYADFMRNLK"
     gene            complement(3303..3845)
                     /gene="def"
                     /locus_tag="VHF05_03660"
     CDS             complement(3303..3845)
                     /gene="def"
                     /locus_tag="VHF05_03660"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVS80054.1"
                     /translation="MVKIVQKDEKVLRQIAEEVPVEEITGPKIQKIIKDMKKALASQD
                     DGVAIAAPQIGHSLRIFVMSGKVLELVRAGEGADEKHEDLVFINPKIINSSKQTHWVE
                     EGCLSVRYLYGHVRRAKKATVEGYDENGKYFTRGGSNLIAQIFQHEVDHLDGVLFIDK
                     AKNLVDIPPEEQAKLEAAGE"
     gene            4198..>4816
                     /gene="dnaX"
                     /locus_tag="VHF05_03665"
     CDS             4198..>4816
                     /gene="dnaX"
                     /locus_tag="VHF05_03665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVS80055.1"
                     /translation="MESTALYRKYRPAAFGDVVGQEAIVAALEAAIERGSVAHAYIFA
                     GSRGIGKTSIARIFANELGTAPEDVYEIDAASNRGIDDVREIREGVAASPFRSKYKVY
                     IIDEVHMLTKEAFNALLKTLEEPPAHAVFILATTELNKVPDTVISRCQVFQFKKPTVQ
                     ILKDVVTEVGKKEGAAIDASSAELIALLGDGSFRDTLGTLQKVLTV"
BASE COUNT         1218 a         1167 c         1238 g         1193 t
ORIGIN      
        1 gttctctact tctgcacttc agaactagtt accatcttct ggttgcttgg cttctgactt
       61 ttctctgccg taatattcta tttgctcttt atttcttacc ttccattcct tatagtcttt
      121 tttcaactga ttaaggaagt cttctagctc ctgtctacta acaccaatta gcattgccgg
      181 aacaaagtct ccgtatgcac ttttttgttc tctccatgga cgaagtttgt gttcattgat
      241 gtagaagcaa aaaacaaagc catcatcctg taacttacca ccagactttt gcctgtcatc
      301 atctactttt tgtaatttgc ccttgtactt gtcgctatac caagctcctt caatttttag
      361 tgcatcttct aaacctaatt ttggattaaa gtgagcatcg attcttactt ctggctcaag
      421 cgacttaaaa actgattcat tgtcttcttg gagcagattt tcaaaaaaag gtattaaata
      481 atcgttttcg tactcatcaa aatgaaaggg atcttcagca agaagctctg gattgattag
      541 cgacttccca ctatacagta accgcaagga atagctaaca tagccgtagt tgtagtctac
      601 gaagttaatc tctgccgcac cgtatttttt atcacctaga attgccatgc ttgaatgata
      661 gcaacttgtc aagcaaagta aagcgataat atttctacac tcttactaaa ttaactccgt
      721 aaagaattat ggatgaatca ctaaggatac ctatgacaaa aagcatcaag agcttcaaga
      781 caaaatgcag gttcttgaaa ttgaactttt ggagcacaga aaagctgata gcgaatacca
      841 aacaaccgtt gctaccgtta tttctgtagc tcgtagggca agagcgattt ttgacggcag
      901 ttcggaacct gctgagaaaa gggcgttttt gaactatcta cttcagaacc cgaccgtgaa
      961 tgcaaaaaag ctagagtttt caatagtttc tcccttcaat ttagtactag aactatcaga
     1021 tagtcctaac ttgctccgcg agtatgaatc tcgaacctta caggttcagt atcccgattg
     1081 gatattttgc ttcaggaact cgcaaaatat gctcaatcgg gcttcgagtc ccacccggaa
     1141 gccccccagg cttctttcgc ggagcaaaca aaaaacgcca caagggcgtt cttagtttgc
     1201 tccgcgagta ggactcgaac ctacaaccag ccgattacat taatcctatt gttgccatta
     1261 gggatggact atatcatctc cctccgtgag gatcgggagc gaggcgcttc gccgtcggct
     1321 attgcctagg ctacttccgt aaggaatagt ctctacacct tcccagctct agttttgatc
     1381 gatagaattt tctataaacc aaaactaggg ctgggctcgg ctcgggatta cccgcgtggg
     1441 gcttccccga attcacctcg tttttcaacc tgaattactt caggaagctg cgtgaccaca
     1501 cagtcggctg ctctaccatt gagctatcgc ggaatgtatt tatgttccaa tcacgctacc
     1561 gttatagtct agcggaacgt gatttacagg gcaaataata gcagaaaagc tatgatttgc
     1621 aacactccag ggcagtgaaa ggcttgggag atttcaccca aaaaacagtt cccggacgta
     1681 tagttttcat atagtacggc gatgcacgca gtgctacaat tatatatata agctaactaa
     1741 actttcgcca tgaatcccgt tgtagaaaat ggcatttaaa agaagcgggt acgtggaata
     1801 aaaattttag tcggatttaa tcactagtta attattcgag aagtgggtaa gttgcgaagc
     1861 aacgatacca tttttcacaa ccggatgaag atcaatacga aggccaccaa tatggagctt
     1921 acccaggcga taaacgatta cgtagcgaaa atggtggagg cgattgagaa attcatagat
     1981 ttgggggacg agagcgcgat tctcgatatc gaggtgggga agacgaccaa ccaccacagg
     2041 acgggtgatt actatcgtgc cgaagtaaat ctcgaatggg caggcaggca gtactactcg
     2101 gcggcggaga aagacgatct ctatgccgcg atcgacgaag tgaaagatca ggttgtttct
     2161 gagcttgcga aatcgaagga taagcatgcc gagaggattc gcgagggcgg caaggaggcg
     2221 aaggaaatgc tccacaagga ccccagcgaa gaggagatcg aggagatgct ttaagaaagc
     2281 ttttagccgg acagcgtata gctcatagct taaagctgga aactttaagg caatagaaaa
     2341 acaaaaaatt ctcattcgca agcatgccat aattctgcag aattatggca tgcttgcgaa
     2401 gagatttgcg agatagtaag aggactttta cttcagattc cgcatgaagt cggcgtagtt
     2461 catctccttc ttgccttcgg ggatgatgcg ccgtacgacg aactgcccgt cgatgagatc
     2521 agcttccttt acgatgacgc ggattttttt gccgtgctgg tcgacaaaga agtacgtgcc
     2581 gggccatggg tcgtgcgcct tgaacttgcg gaagttgaga agcggattgt cggcaaggtc
     2641 gataaggccg tcttccttgg cgaatttttt ggtgaaagct gctttcgaat gatcctgctc
     2701 cttcggcatg accgagccgt ctatccactg cggcaggacc tccgcgagga gcttgccgcc
     2761 ttcgggccag agaagctcgt cgagctccgg tgcgcgagga aggggctcgg gcatcggaaa
     2821 ggatttctgc gcaactatcg gcccatgatc catctccgcg tcgagcgcga tgactgatac
     2881 gcccacattc tcatcgccgg aaagaatctg gctttcgacg ggcgcgggcc cgcgatggag
     2941 cggcaaaagg gaaggatgga cattgagcgt ccccttggca ggaagatcga gaatctcctg
     3001 cggaaggatc tttccgtatc cggcgacgat gaaaagatcg aagttgcggg ctttgagctt
     3061 cgtgaaaaaa tccgtattgc ccctgacttt tgcgggctgt tcgaccggaa tgccgttctc
     3121 ttcggcccac accttcaccg ccggaggagt gaggatatga tcgcggccct gcggcttatc
     3181 aggcgcggta acgacgaggg agggaatgaa tccggcattc ttcagctttt ccaatacggg
     3241 aatgacgaaa gcgggagtgc cgaagaatgc gaagttgagt gtgcgcgggt tcatggatag
     3301 gattattccc cagccgcttc gagcttcgcc tgctcctcgg gaggaatgtc gacgagattt
     3361 ttcgccttgt cgatgaaaag gacaccgtcg agatggtcga cttcgtgctg gaagatttgg
     3421 gcgatgaggt tcgatccgcc gcgggtgaaa tatttgccgt tctcgtcgta cccctcgaca
     3481 gtggctttct tggcgcggcg cacgtggccg taaaggtatc ggacggaaag gcagccttcc
     3541 tcgacccagt gcgtctgttt ggaggaattg atgattttgg gattgatgaa cacgaggtcc
     3601 tcatgcttct cgtcggcgcc ttcgccggcg cggacgagct cgaggacttt gccggacatg
     3661 acgaaaatcc ggagcgaatg cccgatctgc ggcgcggcaa tggccacgcc gtcgtcttgg
     3721 gaggcgagcg ccttcttcat gtctttaatg attttctgga ttttcggccc agtgatctcc
     3781 tcaaccggca cttcttcagc aatctgtctc aggactttct cgtctttctg gactattttg
     3841 accatagggt aattctaagg aagttttcgg aacaaagcaa aaggcggcgc gtggacacgt
     3901 gccgcctcat gaatccggac tatttggacg ctatagaaga ttccaattgt ccggcggtgc
     3961 cgtcgtgcgg actcgagtac gttcgaacga agtacccgac agcgatgata agcaggcaaa
     4021 agaatattgc ctctagaaac aaatgaccca agccttccgg ctgttcgtgt tcagtgtgat
     4081 aacggtatgc gcggcgcact gctctaactc cctccaaatt caataatacc atattgcgag
     4141 gcttagcgta agtgcttggg cgccgcacta gcagcagcct tctgatatac taggctcatg
     4201 gaaagcacag cactctatag gaagtaccgg ccggcggcat tcggcgatgt cgtcggtcag
     4261 gaggcaatcg tcgccgcact cgaggcggcg atcgagcgcg ggagcgtcgc gcacgcgtat
     4321 atcttcgccg gaagccgcgg catcggaaag acgtcgatag cccgcatttt cgcgaacgag
     4381 ctcggcaccg cgccggaaga cgtctacgag atcgacgcgg catcgaaccg cggaatcgac
     4441 gacgtgcgcg agatccgcga aggcgtggcg gcgtccccgt tccgctcgaa gtacaaagtc
     4501 tacatcatcg acgaagtgca catgctcacc aaggaggcat tcaatgcgct tttgaaaact
     4561 ctcgaagagc cgcccgcgca tgcagtcttt attcttgcca cgaccgagct caacaaggtt
     4621 cccgacacgg tgatctcgcg ctgtcaggtc ttccagttca agaagccgac agtccagata
     4681 ctcaaggatg tcgtaacgga ggtggggaag aaggaaggag cggcgatcga cgcatcgtcg
     4741 gcggaactca tcgcgctttt gggcgacgga tcgttccgcg atacgctcgg aacccttcag
     4801 aaagtgctca cggtgg
//
LOCUS       DASZYS010000064         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_638632, whole genome shotgun sequence.
ACCESSION   DASZYS010000064 DASZYS010000000
VERSION     DASZYS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638632"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHF05_03670"
     CDS             <1..333
                     /locus_tag="VHF05_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVS80056.1"
                     /translation="KFLFCMHGTPGAGQPEIDMYDTGVFEEIVPMEKLVYTDSFADKD
                     GNPVSPAAYGMPDMPDVMRVTIELEDAEGGKTKFSLRHDGAPAGQNGTNMQAGWGETL
                     DKLEASLA"
     gene            393..890
                     /locus_tag="VHF05_03675"
     CDS             393..890
                     /locus_tag="VHF05_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVS80057.1"
                     /translation="MSKITPFLWFDKNAEEAINYYVSVFNGNPAKKIESKVITLRRYP
                     EGELEGPMNGFDGKVLTGVFELEGERFMALDGGPIFKFNESVSFLVDCRDQEEIDYFW
                     DTLIKDGGQESQCGWLKDKFGLSWQITPSMEQFLFPSDSEKSKRAMNAMLKMKKIVIA
                     ELENA"
     gene            958..1248
                     /locus_tag="VHF05_03680"
     CDS             958..1248
                     /locus_tag="VHF05_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVS80058.1"
                     /translation="MIIVQDTFVCKPGNASKLAKLCKEVMTGGEVMNIMTDMTGKFNR
                     VVIMCKYENLAAYEESFKKYMENSEEMKKMQEKMAGYTDMYQTGSREIYKTW"
     gene            complement(1313..2170)
                     /gene="cyoE"
                     /locus_tag="VHF05_03685"
     CDS             complement(1313..2170)
                     /gene="cyoE"
                     /locus_tag="VHF05_03685"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003348.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVS80059.1"
                     /translation="MIRDYYELIKPGIIYGNAITAAAGFFLAAQGNFNLSLFIAMLLG
                     LSCVIGSACAFNNLIEIDIDAKMERTKNRALVAGRLDRRDAFWFALALGVCGLSIFAA
                     YVDLLALAVVFLGAVAYLALYTPMKRMNLWSTAAGSISGAVPPVVGYVAVANRLDWAA
                     AILFIILVLWQIPHFYAIGIYRGDEYGAASLPILPVVKGMRAAKVHIALYVAAFTLAT
                     LSLWYFGYAGAAYAAAAGLASLAWLAFAMLGFRKGNNDKPWARKFFLVSLAVLLVISI
                     AISCSGIFA"
     gene            complement(2178..2525)
                     /gene="cyoD"
                     /locus_tag="VHF05_03690"
     CDS             complement(2178..2525)
                     /gene="cyoD"
                     /locus_tag="VHF05_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HVS80060.1"
                     /translation="MTHETTWKNYFLGFGASCILTILSFFIAARHVELGNLSYPVVAA
                     ILLLIAFTQFTVQLVYFLHLGTGKKERMSLAIFLLTLSVVAVIVVGSIWIMNNLNYNM
                     TPQQMDTYSIEQG"
     gene            complement(2535..>3050)
                     /locus_tag="VHF05_03695"
     CDS             complement(2535..>3050)
                     /locus_tag="VHF05_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVS80061.1"
                     /translation="MTDLLMFAAMFATFIVLRGSTFGGVGVADIASIPFVFAETMILL
                     LSSYCIGLAVISAHRGSLKRAALSLSATFVLGAAFLGMEIYEFSRLVAEGHTPAKSAF
                     LSAFFTLVGMHGLHILAGLIWIAIVFPMILRKGFTFRNIERLTLLSMFWHFLDLVWIF
                     IFTIVYLMGVI"
BASE COUNT          822 a          763 c          888 g          577 t
ORIGIN      
        1 aagttcctct tctgcatgca cggcactccg ggcgcgggcc agcccgagat cgacatgtat
       61 gacacgggcg tcttcgagga gatcgtgccg atggaaaaat tggtgtacac cgacagcttc
      121 gcggacaagg acggcaatcc cgtctcccct gccgcgtacg gaatgcccga tatgccggac
      181 gtaatgcggg tgacgatcga gctcgaagat gcggaaggcg gaaagacgaa attctcgctc
      241 cgccacgacg gcgcgcctgc gggacagaac ggcactaaca tgcaggcggg gtggggcgag
      301 acgttggaca agctcgaggc ttcacttgcg tagaacgcgc attgtcggcg catttctttg
      361 ccattcgtta attactatta atttgaatta ttatgagcaa aatcacgcct tttctttggt
      421 tcgataagaa cgcggaggaa gcgatcaact attacgtctc cgtcttcaat ggaaatccgg
      481 cgaagaaaat cgaatcgaaa gtcattaccc tgcgccgcta ccccgaaggc gagctcgagg
      541 gcccgatgaa tggcttcgat ggcaaagtgc ttacgggcgt attcgagctt gaaggcgaac
      601 gcttcatggc gctcgacggc ggcccgatct tcaagttcaa cgagtcggtt tctttcctcg
      661 tcgactgccg cgatcaagag gagatcgact acttctggga taccctcatc aaggacggag
      721 ggcaggagtc gcagtgcggc tggctcaagg acaagttcgg gctctcatgg cagatcactc
      781 caagcatgga gcaattcctt ttcccgagcg attcggaaaa atcgaagcgc gcgatgaacg
      841 ccatgctcaa gatgaagaag atcgtcattg ccgaactcga gaacgcatag caaattaatc
      901 gaaagcgcat ctctccatga gataatgggg gcaattataa aatttaagta aaaaagtatg
      961 atcatcgtgc aagatacgtt cgtgtgcaag ccgggcaacg cctcgaagct tgcgaagctc
     1021 tgcaaggaag taatgacggg cggcgaagtc atgaacatca tgaccgacat gaccggcaag
     1081 ttcaacaggg tcgtcatcat gtgcaagtat gaaaatctcg ccgcatacga ggagagcttc
     1141 aagaaatata tggaaaattc cgaggaaatg aaaaagatgc aggaaaagat ggctggctac
     1201 accgacatgt accagaccgg ctcgagggaa atatacaaaa cttggtagcc acgaataaaa
     1261 aaaccgcttc atgcggtttt ttgatgcctg gaattctgaa ggattcaacc gttcaggcga
     1321 aaattccgct gcaggagatc gcgatcgaga tgacaagaag gacagcgagt gaaacaagaa
     1381 aaaatttccg cgcccagggc ttgtcgttat tccctttccg gaagccgagc atcgcaaacg
     1441 cgagccatgc gagagacgcg agtcccgccg ctgccgcata tgccgcgccg gcatatccga
     1501 agtaccagag cgaaagcgtc gcgagcgtga acgcggcgac atagagggcg atatggacct
     1561 tcgccgctcg cattcccttc actaccggaa ggatcgggag cgaagctgct ccgtattcgt
     1621 cgccccgata gatgccgatc gcgtagaagt gcggaatctg ccacagcacg aggatgataa
     1681 aaaggatggc ggcggcccag tcgaggcgat ttgccacggc aacatagcca actaccggcg
     1741 ggacggcgcc cgaaatactg cctgcggcgg tgctccagag attcatgcgc ttcatcggcg
     1801 tgtagagcgc gagatacgcg acggcgccga gaaagactac agcaagcgcc agaagatcga
     1861 cgtaagcggc gaagatggag agtccgcaga cgccgagcgc taaagcgaac caaaacgcgt
     1921 cgcggcgatc gagccttccc gcaacgagag cgcggttttt tgtcctttcc atcttcgcat
     1981 cgatatcgat ctcgataagg ttgttgaacg cgcaggcgga gccgatgacg cacgaaaggc
     2041 ccaggagcat cgcaatgaaa agcgaaaggt tgaaattccc ctgcgccgct aggaaaaacc
     2101 ccgcggcggc ggttatcgcg ttgccgtaga tgatgccggg ttttataagt tcgtagtaat
     2161 ccctgatcat atgaattcta tccttgttcg atagagtatg tatccatctg ctgaggcgtc
     2221 atattgtaat tgagattatt catgatccag atggagccca cgacgatgac cgccacgacg
     2281 gagagcgtga ggaggaaaat agcgaggctc atgcgctcct tctttccagt gccgagatgg
     2341 aggaagtaaa cgagctgaac ggtaaattgc gtgaaggcaa tgagaagaag gatcgcggcc
     2401 acgacgggat acgaaaggtt gccgagctcg acgtggcggg cggcaatgaa gaacgatagt
     2461 atcgtgagga tgcacgatgc accgaagcca agaaaataat ttttccaagt cgtttcgtgc
     2521 gtcatgattt tgcgttagat cactcccatt aaatatacga tagtaaagat gaatatccag
     2581 acgagatcga ggaagtgcca aaacatcgag aggagcgtca agcgctcgat attgcgaaac
     2641 gtgaatcctt tgcggagaat catcggaaag acgatcgcga tccagatgag gcctgcgaga
     2701 atgtgcagtc cgtgcatgcc gacgagcgtg aagaacgcgg agagaaaggc gctctttgcc
     2761 ggagtatggc cttcggcgac gagcctcgag aactcataga tctccatgcc gagaaaggcc
     2821 gcgccgagaa cgaacgtagc ggaaagcgac agggctgccc gcttcagcga gccgcgatgc
     2881 gccgatatga ctgcgagccc tatgcaataa ctcgacagga gaagaatcat cgtctccgcg
     2941 aacacgaagg ggatgctcgc gatgtcggca acgccaacgc ctccgaaggt gctgccgcgg
     3001 agcacgatga acgtcgcgaa catggccgcg aacatgagga ggtctgtcat
//
LOCUS       DASZYS010000065        10323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_638751, whole genome shotgun sequence.
ACCESSION   DASZYS010000065 DASZYS010000000
VERSION     DASZYS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638751"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VHF05_03700"
     CDS             <1..765
                     /locus_tag="VHF05_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVS80062.1"
                     /translation="KFLFDQGLVPTSEPYAKRTSHGLILAQGGVKMSKSKGNVVNPDD
                     IVEIVGADTLRIYEMFMGPFDQPIAWSTDNMVGSRRFLEKVWKLREKVSSGGEAPPLE
                     LASAVQRAIKKVGEDIESMGFNTAVSTMMILANEFERTSVLPQGDYEALLKLLAPFAP
                     HIAEELWHDLGHAESIHLEKWPAYDPSLLSSGEATIVFQVNGKVRGTVSVEADAEEEE
                     VRKRVLETEEGGKWLGGHEVKRTVFVKNRLINFLLE"
     gene            918..1961
                     /locus_tag="VHF05_03705"
     CDS             918..1961
                     /locus_tag="VHF05_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HVS80063.1"
                     /translation="MKTKATISFKPKQVTKRLLGVLPDRAKDVIVSRYGLGENTERQT
                     LEAIGKKYDITRERVRQIENYSLANIRKSKEYEREQPVFDELKHIVDELGVIVSEDDL
                     LGHLSKDKSAQNHIHFLLVIGEQFTKLKEDDHFKHRWHVDSETAKKIEESLRRMYQGL
                     SDDDLIPESEIIDTFLSQVKDVSDKYKNEEIIRRWLGISKRIDRNPLGEWGRSESPNI
                     HAKGMRDYAFLVIRKHGSPIHFKEVAKAIEQYFNKKAHVATTHNELIKDPRFVLVGRG
                     LYALAEWGYISGVVKDVIKKILDKNGPLTKDEIIDKVLKERYVKENTIVVNLQNPKYF
                     RKDKEGRYHTVEK"
     gene            2296..3552
                     /locus_tag="VHF05_03710"
     CDS             2296..3552
                     /locus_tag="VHF05_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80064.1"
                     /translation="MITDPLTGFSNLFNFNLTSPFAAIGVGLYFVYETSFLWLPIILI
                     WGGWRLWINYKQAKFIFSQKPILLEVRLPLDIVKPPQNMELFLSTLFQTGGEGTWYDR
                     SILGKTRPWFSLELASIEGKVKFYIWSRAAWRNHIEAQLYAQFPGIEVFEVPDYTLPM
                     RFDPEKNILWGCEYALNKPDPFPIKTYQNKDDFSLMLLPGTPTEFQVDPISVVLEYMS
                     SLGPGEQAWLQILMRAHKADRRSTDRRWYNPFSWGKKADWKQTLKQEMKKLQDKEKRS
                     KAEQDALEAMERKLNKLAFETGIRALYIAEKEHFNSTNIAGLSGSFKQYNANNFNGFK
                     PTNTTSVDFPWQDYKNTRVNGKRRVMLDAYKRRSYFFPPYKRHHFILNAEELATIYHF
                     PSAAVTTPGLERIATKKAEPPVDLPI"
     gene            3633..4556
                     /gene="mltG"
                     /locus_tag="VHF05_03715"
     CDS             3633..4556
                     /gene="mltG"
                     /locus_tag="VHF05_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVS80065.1"
                     /translation="MFGAVFAVVAICCVILINPPASFPAGTLFEVPEGGSVTSVAEKL
                     KNDGFIASAFVFRQFVSLFGGQKIIAGDYYFQQKTNVIDLARRITHGIYGLVPLKVTI
                     PEGFNVREIAALLADKLPSFDEAGFISAAQGKEGYLFPDTYYVMPDWSAHEIVDLFNK
                     NFQDKIGNYQSDIASSTHSLSDIVTVASIVEKEASTPEDRRIVSGIIWNRLAKNMPLQ
                     IDATLGYVLNKGTFQLTQSDLKLDNPYNTYLYKGLPPTPIGNPGIDAIEAALHPTKTD
                     YLYYLSDNSGTMHYAKTFEEHKENREKYLGK"
     gene            4661..5851
                     /gene="mnmA"
                     /locus_tag="VHF05_03720"
     CDS             4661..5851
                     /gene="mnmA"
                     /locus_tag="VHF05_03720"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVS80066.1"
                     /translation="MSHSKGKKVFAGMSPIAKEDLATGQAGGSRKKVFVGMSGGVDSS
                     VSAALLKEQGYDVTGVFIKVWSPEFLPCDWKAERRDAMRVAAHLGIPFETLDLEEEYK
                     REVADYMIEEYRRGRTPNPDVMCNRSIKFGGFLKYALENGADFIATGHYCQIQKSKIK
                     NQNDNSKSKTEEIYKLYEGVDKNKDQSYFLWTLTQDQLKHVLFPVGALTKPEVRKEAE
                     RFGLPTAAKKDSQGVCFLGPLDMHDFLAHYIEPKRGDVLNERGEIIGFHDGAFFLTLG
                     KRHGFTITAKTPNDAPLYIVAKDIERNTITVSSERGGSDGEYAVHDIELESVSWTADR
                     PHDGERLLCRFRYRQDAVSCEVLSHEHGLRVHFDEPQSAVSPGQSLVLYRGEECIGGG
                     VIRK"
     gene            6330..7211
                     /locus_tag="VHF05_03725"
     CDS             6330..7211
                     /locus_tag="VHF05_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HVS80067.1"
                     /translation="MEQLKHKAGARRPAASPAEITIVKSTGEREAFDPSKLEASLVRA
                     GASAIARTEIMSRVTRELRDGMTTAQIYRHAFSLLKRMAEHPVAARYSLRRAVMELGP
                     AGFSFEKFVAEIFKEMGYSTKTDLIVRGGCVEHEVDVAAWTSKKLIFVEAKFHNSLGV
                     KSDVKVALYVKARFDDLIGRTFDLGGERKLDEGWLLTNTKFTTTAVEYGLCQGLRMIG
                     WNYPDKGNLQDLIQESGLHPITSLTSLSNKEKSDLLKQGIVLARTLRDEPGHLAALGQ
                     PTGKIKSVLREIEGLLA"
     gene            7222..7707
                     /locus_tag="VHF05_03730"
     CDS             7222..7707
                     /locus_tag="VHF05_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVS80068.1"
                     /translation="MDSDLLTGFITQGLVPFSQLFLALLLGALIGIERTAAGKTAGMR
                     TFSLVSMGSCLFILVSQMSLSTFAVGQVFDPTRLAAGVVTGIGFIGAGLIIFHDNKLK
                     GLTTAAGLWVSCGIGMAVGFQLYAISISATILTLFSFTAMWVLERRIIDDTHSKRKDE
                     S"
     gene            7710..8552
                     /locus_tag="VHF05_03735"
     CDS             7710..8552
                     /locus_tag="VHF05_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVS80069.1"
                     /translation="MAQYYNPKRSADWNYGGSKFKLSRSKIELFMKCPRCFYIDNKLG
                     VARPRSFPLNLNSAVDKLLKKEFDIHRAEKSLHPLMDSYGLDLVPFDHKDMDTWREVF
                     EGVQYFHEPSGLTISGAVDDIWVTPKGELVVVDYKSTSKEEQVNLDADWQVQYKRQLE
                     IYQWLLRRKGFKVSDTGYFVYANGRTDLEAFDGKLEFEVTLIPYTGSDKWVEGTIMDI
                     KKCLDGGEIPAAAADCEYCRYIGAVNGCEPPKKLSAPADKAKAEPRKRQPKDEPSAGL
                     SSLF"
     gene            8567..8857
                     /locus_tag="VHF05_03740"
     CDS             8567..8857
                     /locus_tag="VHF05_03740"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HVS80070.1"
                     /translation="MKTSSQTFAERVRKVVARIPRGKVMTYAEVARRAGNAQASRAVG
                     NIMRKNFDASIPCHRVVRSDGKIGNYNRGGASAKMKRLKEEGVDLERLKLHA"
     gene            8924..9535
                     /locus_tag="VHF05_03745"
     CDS             8924..9535
                     /locus_tag="VHF05_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HVS80071.1"
                     /translation="MDRNDLNKHLKDNSDVLRNDGVKSAFEAVDRADFVGEDYKTEAY
                     EDYALPLGHGQTITKPTIAAFMLELLDLQPGESVLEVGSGSGWMLALISEIIGKTGKA
                     VGVEKIPELLDSARESFKKYDYAHTTMKQAGEAVGHAEAGPYDKILCTASADEIPHEL
                     VSQLKPEGAIVMPVKGKIVRAVKKLGKLEETAYPGEFHFEELS"
     gene            9545..10156
                     /locus_tag="VHF05_03750"
     CDS             9545..10156
                     /locus_tag="VHF05_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVS80072.1"
                     /translation="MNNEAKPQPGDKEIVNFLYEVGMLAKTPRSGFQFLGSGKQSVAE
                     HVNRVAFIGYTLAMLEDGVDVGKVLKMCLFHDIAEGRVSDLNYVNQKYVHADDERAID
                     DMAHNLPFEDDVRKTIGEYEKRESRESVLVKEADILELMLTLKEELDIGNVRAQNWLD
                     RTVKRLKTENGKRLAAMLLRTDSDEWWFADKEDSWWVNRNKSE"
BASE COUNT         2519 a         2799 c         2856 g         2149 t
ORIGIN      
        1 aagttcctct tcgatcaggg gctcgttccg acatcggagc cgtacgcaaa acgcacctcg
       61 cacgggctca ttttggccca aggcggagtg aaaatgtcga aatcgaaggg aaatgtcgtg
      121 aaccccgacg acatcgtaga gatagtaggc gccgacacgc tccgcatcta cgagatgttc
      181 atggggccgt tcgaccagcc gattgcctgg tcgaccgaca acatggtcgg ctcgcggcgg
      241 ttcctcgaga aagtgtggaa gctccgagag aaagtctcct ccggcggcga agcgcctccc
      301 ctggagctcg cgtcggcggt acagcgggcg ataaagaagg tgggggagga tatcgaatcg
      361 atgggcttca acacggctgt gtcgacgatg atgatcctcg ccaacgaatt cgagcggact
      421 tccgttctgc cgcagggaga ctatgaggcc ttactcaagc ttctcgcgcc ctttgcgccg
      481 cacatcgccg aggagctgtg gcatgatcta ggacatgccg aatcgatcca cctcgagaag
      541 tggccggcgt acgacccctc gcttctttcg agcggcgagg cgacgatcgt tttccaggtc
      601 aatggcaagg ttcggggcac cgtgagcgtc gaagcggacg cggaggaaga ggaagtaagg
      661 aaaagggttc tggagaccga agagggcggg aaatggctgg gcggccatga ggtgaaaagg
      721 accgttttcg tgaaaaatcg cctgatcaat ttcctgcttg agtaggccgg gagtggtagt
      781 atttgacaat aaaaaatgta tgtggtaatt ttgtcaatac tatcgccaaa catttgacga
      841 acaggcaagc atttcttgtt gactggacga ggcggaagtg ataaaaatag tataagcaat
      901 actttataac tcatcatatg aaaacgaagg cgacaatttc attcaagcca aagcaggtta
      961 cgaagcggct tctcggagtt ctgcctgacc gcgcgaaaga cgtgatcgtc agccgctacg
     1021 gtttgggcga aaacaccgag cgtcagaccc tggaagccat cggaaagaag tatgacatca
     1081 cgcgcgagcg cgtgcgccag atcgaaaatt actcccttgc gaacatccgc aagtcgaagg
     1141 agtacgaaag ggagcagccc gttttcgacg agttgaagca catcgtggac gagcttggtg
     1201 tcatcgtgtc ggaagacgac cttctcggcc atctctcgaa ggacaagagc gcccaaaacc
     1261 acatccattt ccttctcgtc atcggcgagc agttcaccaa gctcaaagaa gacgaccatt
     1321 tcaagcatcg ctggcacgtc gattccgaga cggcgaagaa gatcgaggaa tccctccgca
     1381 ggatgtacca gggcctctcc gacgacgatc ttattccgga atccgaaatt atcgacacgt
     1441 tcctctccca ggtaaaggat gtctcggaca aatacaagaa cgaggaaatc atcaggcgct
     1501 ggctcggcat ctcgaagcgc atcgacagga acccgctggg cgagtgggga aggagcgaat
     1561 ctccgaacat ccacgccaag ggcatgcgcg actacgcgtt cctcgtcatc cgcaagcatg
     1621 gaagcccgat ccacttcaag gaagtcgcca aggccatcga acaatacttc aacaagaagg
     1681 cccatgtcgc caccacgcac aacgagctca tcaaggaccc ccgcttcgtg ctcgtcggcc
     1741 gcggcctcta cgctctcgcc gagtggggct acatttcggg cgtcgtgaag gacgtcatca
     1801 agaagatcct cgacaagaac ggcccgctca ccaaggatga aatcatcgac aaggtcttga
     1861 aggagcgcta cgtcaaggaa aacacgatcg tggtgaacct ccagaatccg aaatatttca
     1921 ggaaggataa ggagggacgc taccacaccg tcgaaaaata atttacggtc tttttaccgc
     1981 atttcttcgt caaatactcc gtgcgcttcc atggagtatt tttcatacac cttgatcgcg
     2041 ctgctttacc cacggataat ttcctgctgg aaattcccgc gggcccgcct cagcccgtcg
     2101 gcttccggcc ttgcactgct tccagcggtc gcagttcact cctcgaactg cggcaaaaat
     2161 ccttgaaatg cgttggttcg gcagatcgct cccaagcaac tcggtatgtt tgggtttttc
     2221 tgtctactta ctactgtcta ccatctaccg tcttcttaag gtatgattaa tgcattccga
     2281 gcttaggatt ttggcatgat cacggacccg cttacaggat tctcaaacct tttcaatttc
     2341 aatcttacgt cgcccttcgc tgcgatcggg gtggggctct atttcgttta cgaaacgtcg
     2401 ttcctgtggc ttcccatcat ccttatttgg ggcggttgga ggttgtggat caactacaag
     2461 caggcgaaat tcatcttctc gcaaaagccg atcctcctcg aagtgcgcct tccgctcgac
     2521 atcgtgaagc ctccgcagaa catggagctt ttcctctcta cgctgtttca gacaggcggg
     2581 gagggcacgt ggtacgaccg cagtatcttg gggaagacaa ggccctggtt ctctcttgag
     2641 ctcgcttcga tcgagggaaa ggtcaaattc tacatctgga gccgcgcggc gtggagaaac
     2701 catatcgaag cccagctcta cgcgcagttt cccggcatcg aagtgttcga agttcccgac
     2761 tacactcttc cgatgcgctt cgatccggag aagaacatct tgtggggatg cgaatacgct
     2821 ctcaacaaac ccgatccgtt tccgatcaag acataccaga acaaggatga tttctcgctc
     2881 atgcttcttc ccggtacgcc gacggagttc caggtcgatc cgataagcgt cgtgctcgaa
     2941 tatatgagct cgctcggccc cggggagcag gcatggcttc agatactcat gcgcgcccac
     3001 aaggcggatc gccgctcgac cgatcgccgc tggtacaatc ctttctcctg gggcaagaag
     3061 gccgactgga agcagaccct gaagcaggag atgaaaaagc tccaggacaa ggagaagcgg
     3121 agcaaggcgg agcaggacgc cctcgaagcg atggagcgga agctcaataa gctcgcgttc
     3181 gagaccggca tccgcgccct ctacatcgcg gagaaggagc atttcaactc gacgaatatc
     3241 gcaggcctct cgggtagctt caagcagtac aatgccaaca acttcaacgg cttcaagccg
     3301 acgaacacca cctcggtcga ttttccctgg caggattata agaacacgcg cgtgaacggc
     3361 aagcgccgcg tcatgctcga tgcctataag cgccgctcct acttcttccc gccgtacaag
     3421 cgccaccact ttatcctgaa cgccgaggag ctcgccacca tttaccactt cccgagcgcc
     3481 gcggtcacga cgcccggcct cgagcgcatt gccacgaaga aagccgaacc gccggtcgat
     3541 cttcctattt aatgatgcgt ttcaaggtcc cattcgacaa tttcaaaaga ttttgctctc
     3601 gccttttttc gattaggcgg aattcaatct ctgtgttcgg ggcggttttc gctgtagttg
     3661 ctatttgttg cgttatcctc atcaatcctc ctgcgagctt tcccgcgggc acactcttcg
     3721 aagttcccga gggcggcagc gtgacgagcg tggctgagaa gctcaagaat gatggtttca
     3781 tcgcttctgc gttcgttttc aggcagttcg tctcgctttt cggcggccag aagatcattg
     3841 ccggcgacta ttattttcag cagaagacga acgttatcga tcttgcccgc cggatcacgc
     3901 acggaatcta cgggctcgtg ccgttgaagg ttacgatccc cgaaggcttc aatgtgcgcg
     3961 agatcgccgc ccttctcgcc gacaagctgc cgtccttcga cgaagccgga ttcatttccg
     4021 ccgcgcaggg aaaagaggga tatcttttcc ccgatacgta ctacgtcatg ccggattgga
     4081 gcgcacatga aatcgtcgat cttttcaata agaatttcca ggataagatc gggaattacc
     4141 aaagcgatat cgcgtcctcg acccattcgc tctccgacat cgtgactgtc gcgtcgatcg
     4201 tcgagaagga ggcatcgact cccgaagacc ggcgcatcgt ctcgggcatc atctggaacc
     4261 ggcttgcgaa aaacatgccg ctccagatcg acgcgacact cggctacgtc ttgaacaagg
     4321 ggacgttcca gcttacgcag agcgacctga agctcgacaa tccgtacaac acctatctct
     4381 ataagggcct tccgccaacg ccgatcggca atcccggaat cgacgcgatc gaggcggcgc
     4441 ttcatccgac gaagactgat tatctttact atctctccga caatagcggc acgatgcatt
     4501 atgcgaagac cttcgaggag cacaaggaaa atcgggagaa atatttgggg aaatagcggc
     4561 tagtactaag tagataatat ctggaaaatg aaaaaaccgc gcgaacgcgc ggcctttttt
     4621 gcttgcggaa atttgaacca agtaatagca ttatggcgca atgagtcatt cgaaagggaa
     4681 aaaagtcttc gctggcatgt ccccgatagc aaaggaggac cttgctacgg ggcaggctgg
     4741 cggctctcgc aagaaagtgt ttgtgggtat gtcagggggt gtggactctt ctgtaagcgc
     4801 ggcgcttctc aaggagcaag gctacgacgt gaccggagta ttcatcaagg tatggagccc
     4861 ggaatttttg ccgtgcgact ggaaggcgga gcgcagggac gcgatgcgcg tcgcggcgca
     4921 tttgggaatc ccgttcgaaa cgctcgacct cgaggaggaa tacaaaaggg aagtcgcgga
     4981 ctatatgatc gaagaatacc gccgtggcag gacgccgaat ccggatgtga tgtgcaaccg
     5041 ctcgatcaag ttcggcggat ttctcaagta cgcgctcgag aacggcgccg atttcatcgc
     5101 cacggggcac tattgccaaa ttcaaaaatc aaaaatcaaa aatcaaaatg ataattcaaa
     5161 atccaaaact gaagaaatct acaaacttta cgaaggggta gataagaaca aggatcagtc
     5221 ctactttctt tggacgctca cgcaagacca gctgaagcac gtgctgtttc ccgtaggagc
     5281 tctcacgaaa cccgaggtgc gcaaggaagc ggaacgtttc ggcttgccga ccgcggcgaa
     5341 gaaggacagt cagggcgtct gttttctcgg ccctttggat atgcatgatt ttctcgcgca
     5401 ctatatcgag cccaagcgcg gcgacgtctt aaatgagcgc ggcgagatca tcggcttcca
     5461 cgacggcgcg tttttcctca cgctcggcaa gcgccacggc tttactatta ccgccaaaac
     5521 gccgaatgac gcgccgctct acattgtcgc caaggacatc gagcgcaata cgattaccgt
     5581 gagttccgaa cggggaggat cggacggcga gtatgcggta catgacatag agctcgaaag
     5641 cgtgagctgg acggccgatc ggccgcacga cggcgaacgg ctactctgcc gattccgcta
     5701 caggcaggac gcggtttcct gcgaagtgct ttctcatgag cacggcctgc gcgtccactt
     5761 cgacgagccg cagtcggccg tatcgccagg ccagtcgctc gtactctata ggggagagga
     5821 gtgcatcggc ggcggagtga taaggaagta atgagtagct agtagatagt aaatagataa
     5881 ggaacgcccg gaaaccgcct tcgctgtttc cgggcgttcg aaatgagctt acaggtttgc
     5941 ctcagctgtt tattagttgt atagtcgtga aagaaaggac aaaaactttg agaaacaaag
     6001 gtttcttttc agaacttgaa tcatggccgc gtcgtacgaa ggcaaatgaa aagccgccat
     6061 cgagttttcc cgttcagatc ttgcttgccg cagtcggctt gtttgccggc tgcattccag
     6121 tcgcatgcgc tttaaggcaa tttgcgaaaa ttccaaacga tctcaggttt gacggcgtct
     6181 tattgagcgt catcatcagc tgctttgttc tttacgaagt cgtatatcac ataaaatcct
     6241 gagtttcgtt catgcgggga ccgtcgtgtc ctcgcgtttt tttatgcaca gccgcaccca
     6301 gcccgctttt atgcgataat tgaaacgaca tggaacaact gaagcataag gccggcgcgc
     6361 ggcgtcccgc cgcttcgcct gccgagatca cgatcgtcaa atcaaccggc gagcgcgaag
     6421 cattcgatcc gtcgaagctc gaggcgtcgc ttgtccgcgc aggcgcctcg gcgatcgcgc
     6481 gtacggagat tatgagccgc gtgacccgcg agcttcgcga tggaatgacg acggcgcaga
     6541 tctaccggca cgctttctcc cttcttaaaa gaatggccga gcatccggtc gcggcgcgct
     6601 acagcctgcg gcgcgcggtg atggaactcg gccccgccgg attctcattc gagaaattcg
     6661 tagcggaaat cttcaaggag atgggataca gcactaagac cgacttaata gtgcgcggag
     6721 gctgcgtcga gcacgaagtc gacgtcgccg catggacaag caagaagctt attttcgtcg
     6781 aagcgaaatt ccataacagc ctcggagtga agtccgacgt gaaagtggcg ctctacgtga
     6841 aggcccgctt cgacgatctt atcggccgca cgttcgacct cggcggcgaa cgcaagctcg
     6901 acgagggctg gctcctcaca aacaccaaat tcaccacgac cgccgtcgaa tacggcctct
     6961 gccagggact caggatgatc ggttggaatt atcccgacaa gggcaatctc caggatctta
     7021 tccaggaaag cggcctgcac ccgataacgt cgctcacctc gctttccaac aaggagaaat
     7081 ccgatctcct gaaacagggc atcgtgcttg cgcgaacgct ccgcgacgag ccggggcacc
     7141 tcgccgcctt gggccagccg acaggaaaga tcaagagcgt actccgcgag atcgaaggct
     7201 tattagcata atcaaacgca tatggattcc gatttgctta caggttttat cactcaaggg
     7261 ctcgtgccgt tcagccagct cttcctggcg cttcttctcg gagcgctcat cggcattgaa
     7321 cgaacggcgg cggggaagac cgccggcatg aggacgttct cactggtctc gatgggctca
     7381 tgcctcttca tcctcgtgtc gcagatgtcg ctttcgacgt ttgccgtcgg acaggtcttc
     7441 gatcccaccc gcctcgccgc gggcgtcgtg acgggtattg gcttcatcgg tgctgggctc
     7501 attatcttcc atgacaacaa gcttaagggc ctcaccacgg ccgcagggct ctgggtttcc
     7561 tgcggcatcg gcatggccgt cggcttccag ctgtatgcga tctccatttc tgctacaata
     7621 cttacgctct ttagcttcac cgccatgtgg gtgctcgaac gccggatcat cgacgacacg
     7681 cattccaagc ggaaagacga gagttagaaa tggctcaata ctacaatccc aagcgcagcg
     7741 ccgactggaa ctacggcggt tcgaaattca aattaagccg gtcaaagatc gagcttttta
     7801 tgaaatgccc gcgctgcttt tatatcgaca acaagctcgg cgtcgcccgc ccgagaagct
     7861 ttcccttgaa cttgaatagc gccgtcgaca agcttttgaa gaaggagttc gacatccatc
     7921 gcgcggagaa gtccctgcat cctctcatgg attcctatgg cctcgatctc gtgccgttcg
     7981 accacaagga tatggatacg tggagggagg ttttcgaagg cgtccaatat ttccacgagc
     8041 catccgggct cacgatctcg ggcgctgtcg acgatatttg ggtgacaccc aagggcgagc
     8101 tcgttgtcgt cgactataag tcgacttcca aggaggagca ggtgaatctc gacgccgatt
     8161 ggcaggtgca gtacaagcgc cagctcgaaa tctaccagtg gcttctccgc cgcaaaggat
     8221 tcaaggtttc cgacaccgga tacttcgtat atgcgaacgg caggaccgac ctcgaagcct
     8281 tcgacggcaa gctcgagttc gaggtgacgc tcattccgta cacaggctcc gacaaatggg
     8341 tggaaggaac gatcatggac atcaagaagt gcctcgacgg cggggaaatt cccgccgcgg
     8401 cggccgactg cgagtactgc cgctacatcg gcgcggtaaa cggctgcgag cccccgaaaa
     8461 agctttccgc gcctgccgat aaggcaaagg cggagccgag aaaacggcag ccaaaggatg
     8521 agccgagcgc tggcctttcg agcctgtttt aaaaaatttt tgaattatga aaacatcttc
     8581 tcaaacattc gccgagcgcg tcagaaaggt cgtcgcccgg atcccgaggg gcaaagtcat
     8641 gacctatgcg gaagtcgccc gtcgcgcggg caacgcgcag gcgagccgcg ccgtgggaaa
     8701 tatcatgcgc aagaatttcg acgcgagcat cccgtgccat cgcgtcgtcc gctcggacgg
     8761 caaaattgga aactacaata gagggggtgc ttcggccaaa atgaagcgtc tcaaggaaga
     8821 aggcgtcgat ctggaacggc tgaaacttca cgcttaagat ttgaaaaccc ggcataatgc
     8881 cgggttttct tttacctggg gctttatggc ataatgagcg aatatggaca gaaatgacct
     8941 caataagcat ttgaaagaca attccgacgt cctgagaaat gacggtgtga aaagcgcctt
     9001 tgaggccgtc gatcgggccg attttgtcgg cgaagactat aaaacggaag cgtacgagga
     9061 ctacgctttg cctctcggcc acggccagac gattactaag ccgacgatcg ccgcattcat
     9121 gttggagctc ttggatctcc agccgggcga atcggttctg gaagtcggct ccggatctgg
     9181 ctggatgctc gcgctcattt ccgagatcat cggaaagacg gggaaagccg tcggcgtgga
     9241 aaaaattccg gaacttcttg actccgcgcg cgaaagcttc aaaaaatacg attatgccca
     9301 tacgacgatg aagcaggcgg gagaggctgt cggtcacgcc gaagcgggcc cgtacgataa
     9361 gattctctgc acggcgagcg ccgatgagat tccgcacgag ctcgtgtccc aactcaagcc
     9421 agaaggagcg atcgttatgc cggtcaaggg aaagatcgtc cgcgccgtga agaagctcgg
     9481 caagctcgag gagaccgcct atcccggaga attccatttt gaagagcttt cataacgcag
     9541 ggcaatgaac aacgaagcaa aaccgcagcc gggcgataag gagatagtga attttttgta
     9601 tgaagtcggt atgcttgcca agactccgcg gagcggcttc caattcctcg gcagcggcaa
     9661 gcagagcgtt gctgagcacg tgaatcgcgt cgcatttatc ggctatactc tcgccatgct
     9721 tgaggacggc gtcgacgtcg gaaaggtact caagatgtgc ctgttccacg atatcgcgga
     9781 agggcgcgtt tcagatctca attatgtgaa ccagaaatac gtccatgcgg acgatgaacg
     9841 tgcgatcgac gacatggcgc acaaccttcc attcgaggat gatgtacgca aaacgatcgg
     9901 agaatacgag aagcgggaaa gcagggaatc ggttttggtg aaagaagccg atattctcga
     9961 gctcatgctg acgctcaagg aagagctcga tatcggcaat gtgcgggcgc aaaattggct
    10021 agaccgaacg gtaaagcgcc tcaagactga aaacggaaaa aggctcgccg cgatgctctt
    10081 gcggaccgat tcggacgagt ggtggtttgc cgacaaggaa gactcgtggt gggtcaaccg
    10141 caataaaagc gaataatatg aacgcgaaac cgacagaaaa agagatcgaa gcgcggaaag
    10201 cagcgctcaa gaaaatccgc gacgaagtgt tcgagctcac aagctcgccg ctctatccgg
    10261 agcgtacgaa gaacaaagtc tacccggtga tcggggaggg aagccacttc gcggagataa
    10321 tgt
//
LOCUS       DASZYS010000066         9624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_892452, whole genome shotgun sequence.
ACCESSION   DASZYS010000066 DASZYS010000000
VERSION     DASZYS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892452"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1335
                     /locus_tag="VHF05_03755"
     CDS             226..1335
                     /locus_tag="VHF05_03755"
                     /inference="COORDINATES: protein motif:HMM:NF018287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HVS80073.1"
                     /translation="MEYKGALPSQLIQKLLSGGFIEGAIMENVRPASIDLTVENEGYR
                     VDGAFLPSNEERVEMALKRAGAIPHACGATLEKGCCYVFRLKESIVELPTGVYGYSNP
                     KSSSGRLDIHVRLLADRVAGYDSIPNGYSGPLWMMISPKKFSVILREGITLLQLRFFN
                     QDTRFDELRLRLNFEADGGFLMNGEGQMIRYKDQAYSHHDGSIVLTLGLCYEMPGFEA
                     IETGVPIDLSLVNHYDPEEFFRPITVKRNSIALAVNAFYILSTREYVRVPRNLSCEMV
                     PMDARNGELRSHYAGFIDPGWGIGIDGSGKGRPLTLEVRSFDSSLIIQNGQPIADIRF
                     ERMIEEPHAHYDQMSPHYGDQNGPKLGKHFKPWTK"
     gene            complement(1382..2632)
                     /locus_tag="VHF05_03760"
     CDS             complement(1382..2632)
                     /locus_tag="VHF05_03760"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HVS80074.1"
                     /translation="MLQALHSRSIGGLMHHLGVLAKKGAKNFMEAFYVGYGHKSIGDC
                     GDATVFVEGVSMLVAKAIQDWRLYCGQEASTRYIDFANQRFLNPLGTPEGERILENWR
                     RFYLKGLGILKHELSFMFPRGETDDEKSYQKAINARAFDIMRGFLPAGATTNLAWHAN
                     LRQLGDQLDLLRHHPLEEVRDVAEAVESALSEKFPGSFPRKRYPASEAYNKQIMHDFT
                     YFAPDYVDWFRTKDVSINEPLLRKYRTALEDRPPKTELPRKIAECGTLQFEFLLDFAS
                     FRDIQRHRAIIQQMPLLTTNFEFHPWYLSQLPEMLRVEAEGVLATQELGIASLGASPE
                     ISQYYTAMGFQIPNKLTGDIHALTYLVERRAGNDVHPTLRERAFQIAKALQRELGDYG
                     LILHLDDEPHRFDIKRGTQDIVLK"
     gene            complement(2837..3271)
                     /locus_tag="VHF05_03765"
     CDS             complement(2837..3271)
                     /locus_tag="VHF05_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80075.1"
                     /translation="MTHVDLKRIRDEKQLKTMREIEQAGHCPFCGDNLLLYHKEPILK
                     ETERWLLTKNQWPYKNSRVHLLAIYKDHKTHIDQIPPEAGYELFDLMRWAVQEYDMRG
                     GAVVWRFGDTKYTGGSVSHLHAQLVTADVDAPGYEPIRFFLG"
     gene            3335..4519
                     /gene="glyA"
                     /locus_tag="VHF05_03770"
     CDS             3335..4519
                     /gene="glyA"
                     /locus_tag="VHF05_03770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVS80076.1"
                     /translation="MKDRDVADLIGLEKKRQKSVVNLIASENYVSKDVLEALGSELTN
                     KYAEGYPSARYYGGNGVVDKIEELAKKRALKAFGLSAKKWHVNVQGLSGSPANLAVYS
                     ALVPLGEKIMGLSLDHGGHLTHGHKVSLTGKLWAQVPYGVGKETEKLNYVEIERIAKK
                     ERPSLIVAGFTAYPRKIDFKKFREIADTCGAMLMIDMSHFAGLVAGKAYQSPFPYADV
                     VTTTTHKTLRGPRAALIFSRIDSRELYKKIDKAVFPGLQGGPHVNAIAAVAVALNEAA
                     TPEFKKYAKQVVANAKALAETLKKLGWRLVSGGTDTHLVLVDTWMNGEGIGGKEASEA
                     LEKARIIVNKNTIPFDTRMPVDPSGIRLGTAAETTRGAKEADMVEIARKIDRILRAAS
                     SH"
     gene            4571..5209
                     /locus_tag="VHF05_03775"
     CDS             4571..5209
                     /locus_tag="VHF05_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80077.1"
                     /translation="MIDGSDGSGKRTQAELLQAYFESKGEEALLVSFPRYQEFFGKLL
                     RDLLDGKEGDFISVSPRLASLAYAADRLDAASEIRAALRSGKHVICDRYVSANQIHQG
                     GKISDVSKRIEFLEWLDVMEYRKFGVPRPDISIYLDVPVAISLELMADKAKDAAESNI
                     AHLQASVETAKWLIKHEPDKWIHIECAPWGKMISRKAIHEELVHQLNLKAIR"
     gene            5299..5721
                     /gene="dut"
                     /locus_tag="VHF05_03780"
     CDS             5299..5721
                     /gene="dut"
                     /locus_tag="VHF05_03780"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVS80078.1"
                     /translation="MKLKVKKLHPDAVVPQFAHDDDAGMDLFALERTEALPHSVTRIR
                     TGIAMEVPEGYAGLCWDKSGLSTNHGLKTLAGVLDAGYRGELVLAVYNLKDEPYVYEK
                     GHKVMQILIQKIEHPEIVEVDELTETVRGAGGFGSTGK"
     gene            5743..7452
                     /gene="argS"
                     /locus_tag="VHF05_03785"
     CDS             5743..7452
                     /gene="argS"
                     /locus_tag="VHF05_03785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVS80079.1"
                     /translation="MSIREEIQKAIAEAVSELGLKPEEILIDHPSDFTMGDLTTNVAM
                     MKAKALGANPRELAEKIKSALEGKIADVEKIDVAGPGFINFHLSKDFFNKKIEEVLSD
                     PQFGANEMLKGRKVMLEYTDPNPFKQFHIGHLMSNAIGESLSRLYEWSGAEVKRANYE
                     GDTGLHVAKAIWGIRKKMSEKPVDDAPLSEQVAFLGNAYTFGSNSYEDDAAAKAEIDE
                     LNRVIFLKADPELNALWEWGKKVSLDHFEEIYAKLGTKFDYYFFEDKVANRGLEIVNE
                     GLAKGIFEKSDGAVVFRGENHGLHTRVFINSKGLPTYEAKELALAEKKNETYSFDESI
                     SITASEQNDYFRVVLKALELINPELRAKIRHISHGMLRFASGKMSSRKGNVITGESLI
                     AAMQEMVYDKMKDRPMANDEKNEISEEVGVAAIKYSILKQAPGRDIIYDFDKSLSFDG
                     DSGPYLQYATVRARSVLDKARQEGIAASVERPDDEVAEVAKLVSRFPDVVLRAERELM
                     PSYLVTYLIELAANFNSYYADHKIVDAKDAHSPYRVALTSAFTRVMENGLYLLGMKVP
                     VKM"
     gene            7788..>9624
                     /locus_tag="VHF05_03790"
     CDS             7788..>9624
                     /locus_tag="VHF05_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVS80080.1"
                     /translation="MENKDTHKSAEKSAVAAREEEILKFWRDNDIFRKSLEKPSPKGN
                     YVFYDGPPFATGLPHFGHMLPTTIKDAIPRFRTMQGYHVMRRWGWDCHGLPIENLIEK
                     ELGLDTKKDIEEYGIEKFNVAARGKVLQYADEWKEIIPRLGRWVDMENDYRTMDSSYT
                     ESVWWSWKQLNEKNLVYRSFKVMYICPRCETTLSNFEVNQGYKDIADISVYARFKVKN
                     QNAKIKNIIEKSKIADLYLLAWTTTPWTLPGNVALAVKNDIAYSVVKNSKENGEAELV
                     VIAQPLFEKLKGKFSEPEIVGELKGSDLVGTEYEPLFDYYANDSKLKNRENGWKVIAA
                     DFVTMEEGTGIVHVAPAFGAEDFEAAEKENLPFVQHVTMHGVFKPEVKDFAGLLVKAK
                     DSPEDPNAHQATDIEIIKFLAAHGTLFAKEKITHSYPHCWRCSTPLLNYATTSWFVKV
                     TALKDTLLKNNGTVSWVPEEIGEGRFGKWLDGARDWAVSRSRYWGAPLPVWICEKCET
                     QEFAGSVDELSSKIGRGNTFVAMRHGESENVVKEITSSDNSVPSHLTDAGKRQVREAA
                     EKLKANGITAIYASPLFRTKETAEIAADVLGLDPSKIIYDDRLKEI"
BASE COUNT         2374 a         2480 c         2672 g         2098 t
ORIGIN      
        1 ggcaccgcca ggagcatggg ggtcgacgtg aaataagaaa catcgaccgg ccggacccgc
       61 cttgggcggg gaggcgggcc cgcgcaattt ttcagcagaa aaatatgcgt gggtggaggt
      121 aaaaggctaa ttctatttaa aagaaaaccg cacatatgcg cggttttctt ttttgcgtat
      181 tcgtgttaga aaggctctag aattttgtca tagaaaggat tgaaaatgga atataaaggc
      241 gcattgccga gccaactcat tcaaaagctt ctcagcggcg gttttattga gggggcgatt
      301 atggaaaatg tccgtcccgc atcgatcgac ctcacggtcg agaacgaagg atatcgtgtc
      361 gacggcgcgt tcctgccgag taatgaagaa agggtagaaa tggcgcttaa gcgcgcagga
      421 gcgattccgc atgcatgcgg ggcgactctt gaaaaagggt gctgctacgt cttccggctc
      481 aaggaatcga tcgtagaatt gcccacaggt gtatatgggt attcgaatcc gaagtcatcg
      541 agcggcaggc tcgatataca tgtccgactc ttggctgatc gcgttgcagg atacgattcg
      601 atccccaacg ggtattccgg gccgctctgg atgatgattt ctcccaagaa attctcggtt
      661 attctccggg agggaatcac gcttctgcag ctgcgattct tcaatcagga tacccggttc
      721 gacgaactgc gtcttcgcct taatttcgaa gcagatggcg ggtttctcat gaacggcgaa
      781 ggccaaatga tcagatataa ggatcaggcg tattctcacc acgacggatc gatcgttctg
      841 accctgggtc tctgctatga gatgccggga ttcgaagcga tcgaaacagg ggttcccatc
      901 gacctctcgc tcgtaaatca ttacgatcct gaagagttct tcaggccgat tacggttaaa
      961 cggaacagta tcgcgcttgc ggtaaacgct ttctacattc tttcaacgag ggagtatgtg
     1021 agagtgccga ggaacctctc gtgcgaaatg gttccgatgg atgcccgaaa cggcgaactc
     1081 cgctcgcatt atgcggggtt catcgatcct ggatggggaa tcgggattga cggaagcggc
     1141 aagggtcgcc cgctgacgct cgaagtccgc tcattcgaca gcagccttat cattcaaaac
     1201 ggccaaccga tcgccgatat acgcttcgaa cggatgattg aggagcctca cgctcactac
     1261 gatcagatgt cgccgcacta cggggatcaa aacggcccca aactcggtaa gcatttcaag
     1321 ccgtggacaa aataagtaca aaaatactcc gtgtaatctt ttagggatta cacggagctt
     1381 tttatttgag aacaatatcc tgagtcccgc gtttaatgtc gaagcggtgc ggttcatcgt
     1441 ccagatggag aatgagtccg taatcgccga gttctctttg gagagctttg gcgatttgga
     1501 aagcccgttc tcgaagagtg ggatggacgt cgtttcctgc gcgccgttcc accaaatagg
     1561 tcaatgcgtg gatatcgccg gtaagcttgt tcggaatttg gaaccccatt gccgtatagt
     1621 actgtgatat ttccggtgag gcaccgagtg aggcaattcc gagctcctga gtggcgagta
     1681 cgccctcggc ttccactctt agcatttcag gtaattggct gagataccag ggatggaatt
     1741 cgaagttagt ggtcagaagc ggcatctgct ggatgatagc cctgtgccgc tggatgtcgc
     1801 ggaaggatgc gaaatcgagc aggaactcga actgcagcgt gccgcattcg gcgatttttc
     1861 gcggcagttc tgtttttggc gggcggtctt cgagtgcggt gcggtatttc ctgagcagcg
     1921 gttcattgat cgatacatcc ttcgttctga accaatcgac ataatctggc gcgaagtatg
     1981 tgaaatcatg catgatttgc ttattatatg cttcgctcgc agggtatctc tttcggggaa
     2041 acgatccagg aaatttttca gaaagcgcgc tttccacggc ttctgcgaca tccctcactt
     2101 cttcaagcgg atgatgccgc aacaggtcaa gttgatcgcc aagctgtcga agattcgcat
     2161 gccatgcgag attggttgtc gctcccgctg gcaggaatcc gcgcatgatg tcgaatgcgc
     2221 gcgcattgat cgccttctga tagcttttct cgtcatcggt ctctccgcga ggaaacataa
     2281 aggaaagctc atgtttgagt atccccagac ccttgagata gaatcttcgc caattctcaa
     2341 gaatccgctc tccttctggc gtgccaagcg gattcaaaaa tcgctgattt gcgaaatcaa
     2401 tgtagcgtgt agaggcttcc tgcccgcaat agagacgcca atcctgaatc gccttggcga
     2461 cgagcattga gacgccttcg acgaatactg tcgcatctcc gcaatcgccg atggatttgt
     2521 gtccatagcc gacgtagaac gcttccatga aattcttagc gccttttttt gcgaggacgc
     2581 cgagatgatg catgagaccg ccgatcgaac gggaatgcaa tgcctgaagc atcgcttctt
     2641 cttcaggacc gataatggcg ccggtatcga gtacgatgac atgacctccg tttttaagcg
     2701 gacgcatgac gtgcttaagg tcgtctaaac ccattattat tctccttgtt tgatgatatg
     2761 gtacagaaac tgacgctagc ttaccgggtt tggctgaaaa tgcaactgga gagatgttgc
     2821 gcgcgtagcc aggactttat cccaaaaaga accgtatcgg ctcgtatccg ggagcgtcga
     2881 cgtctgccgt cacgagctga gcgtgaaggt ggcttaccga gccgcccgtg tacttcgtat
     2941 cgccgaatcg ccacaccacg gctccgcccc gcatatcata ttcttgcacg gcccatcgca
     3001 tgagatcgaa aagttcgtac cccgcttcgg gaggtatctg atcgatatga gtcttgtgat
     3061 ccttgtaaat tgccaggaga tggactcgcg aattcttata cggccattgg tttttggtga
     3121 gaagccaacg ttcggtttcc ttgaggatcg gctccttgtg ataaagaagc aggttatcgc
     3181 cgcagaaagg gcagtgtcct gcctgttcga tctcccgcat cgttttgagc tgtttctcgt
     3241 cgcgaatgcg tttaagatcg acgtgcgtca tgtctccaag gtatcatgat gcgcgcttct
     3301 gtccaaacat gcgttcattt gatatattca gcccatgaaa gacagggacg tcgcggatct
     3361 catagggttg gaaaagaagc ggcagaagtc ggtcgtgaat cttatcgcgt ccgaaaacta
     3421 tgtctcgaaa gacgtgctcg aggcgctcgg aagcgagctt accaacaagt acgccgaagg
     3481 ctacccgtca gcccgctact acggcggcaa tggggtggtg gacaagatcg aggagcttgc
     3541 gaagaaacgc gcgctcaagg cgttcggatt gtcggcaaaa aaatggcatg tgaacgtcca
     3601 agggctgtcc ggctctccgg caaacctggc agtctacagc gctctcgtgc cattgggaga
     3661 aaagatcatg ggactctcgc tcgatcatgg cgggcacctc acgcacggcc acaaggtgtc
     3721 gctcacgggg aagctctggg cacaggtgcc gtatggtgtc gggaaggaaa cggagaagct
     3781 caactatgtc gagatcgaac ggatcgcaaa aaaggagagg ccctcgctca tcgttgcggg
     3841 cttcacggca tatccgcgga aaatagattt caaaaaattt cgcgaaatcg cggacacttg
     3901 cggcgcgatg ctcatgatcg atatgtcgca tttcgcaggg ctcgtggcgg ggaaggcgta
     3961 tcagtcgccg ttcccgtatg cggacgtcgt taccacgacc acccacaaga cgctccgcgg
     4021 cccgcgcgcg gcgctcatct tctcgcgcat cgattcgcga gaactctaca agaaaatcga
     4081 caaggccgtt tttccgggac tccagggcgg cccgcatgtg aatgctatcg cggcggttgc
     4141 agtggcgctt aatgaggccg ccacgcccga gttcaagaaa tacgcgaagc aggtcgttgc
     4201 gaacgcgaaa gcgcttgcgg aaactttaaa gaagctcggt tggcggctcg tctcgggcgg
     4261 cacggatacg cacctcgttc tcgtcgatac atggatgaac ggggagggaa tcggcggcaa
     4321 ggaggcgagc gaggcgcttg aaaaagcccg cattatcgtc aacaaaaata ccattccgtt
     4381 tgatacccgc atgcccgtcg atccgtcggg cattcgcctc ggcactgccg ccgaaacgac
     4441 acgcggcgcg aaagaagctg acatggtgga aattgccaga aaaatagacc gcattttacg
     4501 cgctgcgtct agtcattaaa atctctgctc tcgcgtatac tgccccgcag gaggcagtca
     4561 gatgttctat gtgatcgatg gcagcgatgg ttccggcaag cgtacccagg cggaattatt
     4621 gcaagcatat tttgaaagca agggagaaga ggcgcttctt gtaagctttc ctcgatacca
     4681 agaatttttc ggcaagctct taagagactt gctcgatggg aaggaaggcg atttcatttc
     4741 cgtaagcccg cgtttggcat cgctagcata tgctgccgat cgtttggatg cggcatccga
     4801 aattcgagct gcgttgcggt cgggtaagca cgtcatttgc gatcgttatg tgagtgcaaa
     4861 tcaaatccat caaggtggta aaatttctga tgtatccaaa cgcatcgaat ttcttgaatg
     4921 gctggatgta atggaatacc gaaaatttgg tgtcccccgt cccgatatca gcatctatct
     4981 ggacgtaccg gtggcaatct cactcgaact catggccgat aaggcgaaag atgccgcaga
     5041 aagcaatatt gctcacctgc aagcttcggt cgaaacggcg aaatggctca tcaaacacga
     5101 gcctgataaa tggattcaca ttgagtgtgc tccatggggg aaaatgattt ctcgaaaagc
     5161 cattcacgaa gagcttgtcc atcagctcaa cctcaaggcg atccgataat tcgggtcgcc
     5221 tttcaatgtg atggctgatg ccgtttgtag tactcagcaa acttttattt ttatctaaag
     5281 ctgctgtatt atggcggcat gaagctcaag gtcaaaaaac tccatccgga cgcggttgtg
     5341 ccgcagttcg cgcatgacga cgatgcaggt atggacctgt ttgcacttga acgcacggaa
     5401 gctcttccgc attctgtaac gcgcatccgt acgggtatcg ccatggaagt gccggaggga
     5461 tatgcaggcc tttgttggga caaaagcggg ctctcaacca atcacggcct taaaaccctt
     5521 gcgggagtac tcgatgcggg atacagggga gagctcgtgc tcgcggtgta caatctcaag
     5581 gatgaaccgt atgtgtatga gaagggccac aaggtgatgc agatccttat ccagaagatc
     5641 gaacaccctg agatcgtcga agtcgacgaa cttacagaaa cggtccgcgg cgcaggcggc
     5701 ttcggcagta cgggaaagta agtgtgggta tacagtataa gtatgagtat tagggaggag
     5761 atacaaaaag cgatcgcgga agcggtgtcg gagctcggat tgaagccgga ggagatttta
     5821 atcgatcatc cgagcgactt cacgatgggc gaccttacga ccaatgtcgc catgatgaaa
     5881 gccaaagcgc tcggcgcgaa cccgcgcgag ctcgcggaga aaataaaatc ggcactcgaa
     5941 ggaaagatcg ccgacgtcga gaaaatcgat gtcgccggtc cgggcttcat caatttccac
     6001 ctctcgaaag attttttcaa taaaaaaata gaagaagttt tgagcgaccc gcaattcggc
     6061 gcgaatgaaa tgcttaaggg ccgcaaagtc atgctcgaat acaccgaccc gaatccgttc
     6121 aagcaattcc acatcggcca cctgatgtcg aacgccatcg gcgaatcgct ttcgcgcctc
     6181 tacgagtgga gcggcgcgga ggtgaagcgc gcgaactacg aaggggacac cggcctccat
     6241 gtcgccaagg cgatctgggg aatccggaag aagatgagcg aaaagcctgt cgatgatgcg
     6301 ccgctttccg agcaggtggc gtttctcggc aatgcgtata ccttcggctc gaacagctac
     6361 gaagacgacg cggcggcaaa ggcggaaatc gacgagctga accgcgtcat cttcctcaag
     6421 gccgatccgg agctgaacgc tctctgggag tgggggaaaa aggtgtcgct cgaccacttc
     6481 gaggagattt atgcgaagct cggcacgaaa ttcgactact actttttcga ggacaaagtg
     6541 gcgaaccgcg gccttgaaat cgtgaacgag ggcctcgcga aggggatttt cgagaaaagc
     6601 gacggcgcgg tcgtcttccg cggcgagaac cacggcctcc atacgcgcgt attcataaat
     6661 tcaaagggcc tgccgaccta cgaggcgaag gagctcgccc tggccgagaa gaaaaacgaa
     6721 acctactcct tcgacgagtc gatctcgatc accgcaagcg agcagaacga ctacttccgc
     6781 gtggtcttga aggctctcga gctcatcaat ccggaactcc gcgcgaagat ccgccacatc
     6841 tcccacggca tgctccgctt cgcctcgggc aagatgtcgt cgcgcaaggg caatgtcatc
     6901 accggcgaat cgctcattgc cgccatgcag gaaatggtct acgacaagat gaaggatcgc
     6961 cccatggcaa acgacgagaa gaacgagatc tccgaagagg tcggcgtcgc cgcgatcaaa
     7021 tactcaatac tcaagcaggc tcccggtcgc gacatcatct acgatttcga caaatcgctt
     7081 tcattcgacg gcgactcggg gccgtacctc cagtatgcga cggtgcgcgc ccgctccgtt
     7141 ctcgacaagg cgcggcagga ggggattgcc gcgtcagtcg agcggccgga tgacgaggtc
     7201 gcggaagtgg cgaagcttgt ctcgcgcttt cccgatgtcg tcctgcgcgc ggagagggag
     7261 cttatgccga gctatctcgt aacgtacctt atcgagctcg ccgcgaactt caacagctat
     7321 tacgccgatc acaagattgt cgacgcgaaa gacgcgcatt cgccctatcg cgtcgcgctc
     7381 accagcgcgt ttactcgcgt gatggaaaac ggcctgtatc ttctgggcat gaaagttccg
     7441 gtcaaaatgt agggcgggaa aattcggcgt atttcttcgt tgctcgcgaa atttgctccc
     7501 gcgcagtact agcatgtaca gcttggtcgc aaatttcgct cgcgccttga actgcatccg
     7561 aattatcccg ccctaaaagc cccatttcct ccttttgatg atttttgaaa gaagtaccaa
     7621 aaatgctagg ataggaattg cgcaatgaat tccaggatcc cgttgtagaa aatggcattg
     7681 gggaaaaata actgggatat atttctataa taaggtcgaa ttgctgttaa taaccgaata
     7741 tgaagacggc ccgctcccca aagagcgatg ccatttttca caacaggatg gaaaataaag
     7801 acacacacaa atcggccgag aagagtgccg tagcggcgcg cgaggaggaa atcctcaagt
     7861 tttggcgcga caacgacatt ttccggaagt ccctcgagaa gccttcgccc aaaggcaatt
     7921 atgtttttta tgacggcccg cctttcgcga ccggccttcc tcacttcggg cacatgcttc
     7981 cgacgaccat caaggacgcg atcccgcgtt tccgcaccat gcaaggctac catgtcatgc
     8041 gccgctgggg ctgggactgc cacggcctgc cgatcgaaaa tctcatcgag aaggagctcg
     8101 gcctcgatac gaagaaagac atcgaggagt acggcatcga aaaatttaac gttgccgccc
     8161 gcggaaaagt tctccagtac gccgacgaat ggaaggaaat cattcctcgc ctcggtcgct
     8221 gggtggacat ggagaacgac taccgcacga tggattcctc atacaccgaa tcggtctggt
     8281 ggtcgtggaa gcagctcaat gaaaagaatc tcgtctatcg cagcttcaag gtgatgtata
     8341 tctgcccgcg ctgcgagacg acgctctcga atttcgaggt caatcaggga tacaaggata
     8401 tcgccgatat ttcggtatac gcgagattca aggtcaaaaa tcaaaatgca aaaatcaaaa
     8461 atataattga aaaatcaaaa atcgcagacc tgtacctctt agcttggacg accactccct
     8521 ggactcttcc gggtaatgtc gcgcttgccg tcaagaacga tatcgcgtat tcggtcgtga
     8581 agaattcgaa agaaaatggc gaagccgaac tggttgtcat cgcacagccc ctcttcgaaa
     8641 aactcaaagg aaaattcagc gagccggaaa ttgtcggcga attgaaaggt tcggatcttg
     8701 tcggtacgga atacgaaccg cttttcgatt actacgcgaa cgactcgaag ctcaagaacc
     8761 gcgagaacgg ctggaaagtc atcgcagccg atttcgtgac gatggaggag gggaccggca
     8821 tcgtacatgt cgcgcccgcg ttcggcgcgg aggacttcga ggccgcagag aaggaaaatc
     8881 tgccgttcgt ccagcatgtg accatgcatg gcgtcttcaa gcccgaggta aaagacttcg
     8941 cgggccttct cgtgaaggcg aaggattcgc cggaagatcc gaatgcacat caagcgaccg
     9001 acatcgaaat catcaaattc ctcgccgcgc acggaacgct tttcgccaag gaaaaaataa
     9061 cgcactcata cccgcactgc tggcgctgtt cgaccccgct cttgaattac gccaccacgt
     9121 catggttcgt gaaggtgact gcgctcaaag acacgcttct caaaaacaac gggaccgtca
     9181 gctgggttcc ggaggagatc ggcgaaggcc gtttcggaaa atggctcgac ggcgcccgcg
     9241 actgggcggt gtcgcgttcg cgctactggg gcgccccgct tccggtctgg atctgcgaaa
     9301 aatgcgaaac gcaggaattt gccggctcgg tcgatgagct ctcgtccaag atcggccgcg
     9361 gcaatacgtt tgtcgctatg cgccacggcg aatcggaaaa cgtggtgaag gagatcacga
     9421 gctccgataa ttccgtgccg agccatctga cggacgcagg caagcggcaa gtccgcgaag
     9481 cggcggagaa gctcaaggcc aacggcatta cggcgatcta tgcgtcgccg cttttccgca
     9541 cgaaggaaac cgccgagatc gccgccgacg ttctagggct ggacccgtcc aagattatct
     9601 acgacgaccg cctcaaggag atca
//
LOCUS       DASZYS010000067        15529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U6216
            k141_449714, whole genome shotgun sequence.
ACCESSION   DASZYS010000067 DASZYS010000000
VERSION     DASZYS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957029
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:46:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 773
            CDSs (total)                      :: 743
            Genes (coding)                    :: 741
            CDSs (with protein)               :: 741
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15529
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449714"
                     /isolate="SMAG_U6216"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..348
                     /locus_tag="VHF05_03795"
     CDS             16..348
                     /locus_tag="VHF05_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80081.1"
                     /translation="MFEGYSPNNNEIRPSSPDQAETFGTLEGEDAFEKAMSQILDDLP
                     GASDIIQPRDYQGILEEAKVTDQEKGILEEIATKLPTIEEAQIAQPIQEKIKNAVLKR
                     LEEMEAAV"
     gene            complement(595..912)
                     /locus_tag="VHF05_03800"
     CDS             complement(595..912)
                     /locus_tag="VHF05_03800"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVS80082.1"
                     /translation="MHPTEKVLKTIKFKHMNKQAIVEKVHEKLGGTKVQAEQAVDTVV
                     EAIINTLKKGEEVSIAGLGIFSTKTRASRQARNPRTGETISVPSMRVPKFRPAKALKD
                     AVK"
     gene            complement(1551..3074)
                     /gene="rny"
                     /locus_tag="VHF05_03805"
     CDS             complement(1551..3074)
                     /gene="rny"
                     /locus_tag="VHF05_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HVS80083.1"
                     /translation="METTVILIIAAGIAGIAFGYFLRIFIALKKKNSIELDIKTLMLD
                     AKEQEARLLEEATKKSDDLLREARAEIKEKDEKLKRTEERLIKKEELLDKRQTDIDNE
                     VEDIKERIEKIKELRQRADDLIAERTKELERVAKFSLDEAKKELETIAEKEAEEDLMV
                     RLQKLETFGAEQLERKAKDILTTVIHRLANSVSPDIMTTSVVIPNDEIKGKIIGKEGR
                     NIKAFERATGVEVIVDDTPGAITLSCFDPVRRQVARLALENLVLDGRIQPAKIEETVE
                     KSKQEINKIIKEKGEAAVFEAGVYNLDPRLVSILGRLYFRTSYGQNVLQHSVEMSHLA
                     GMLAEELGANIAVAKAGALLHDIGKALDHEVAGTHVEIGRRILQKFNVSEDIIKAMQA
                     HHGEYPFETPESIIVQVADAISGGRPGARRDTVENYLKRLADLEATANSFAGVEKSYA
                     LQAGREIRVFVTPDAVSDLEARKMARDIALRIESELKYPGEIKVSVIRETRVIEFAR"
     gene            complement(3439..4017)
                     /locus_tag="VHF05_03810"
     CDS             complement(3439..4017)
                     /locus_tag="VHF05_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HVS80084.1"
                     /translation="MSYLIPTVIEKSQFGERAYDIYSRLLRERIIFLGGPIDDDTANI
                     VIAQLLFLASEDAKKDISLYINSPGGSVTAGLAILDTMNHVAPDVSTVCVGIAASAAA
                     VILSAGAKGKRFSLPNSEVMIHQPHIQGVGGQATDIEITAKQILKSRDQLNKILAKNT
                     GKPVSQIEKDVDRDYFLDADEAKKYGLIDKII"
     gene            4491..5183
                     /locus_tag="VHF05_03815"
     CDS             4491..5183
                     /locus_tag="VHF05_03815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HVS80085.1"
                     /translation="MRLHRFFIDQEIRDAKDVVVTDRDLLNQWKNVFRLGKEDRVILL
                     DGSGFEFEAEFALLARDRAELAILAVKKAANVSERNITLYASLIKKDNFEWVLEKGTE
                     LGVAGFVPLQTERSEKKDLNIERAKRIIREASEQSGRGMEPELYAVDTLADAVGNASM
                     KLVAFDPTGAELDRNSLRESEIGILVGPEGGWSERELAFFKEKNISVYTLGPQVLRAE
                     TAAIAISTLALL"
     gene            5243..5566
                     /locus_tag="VHF05_03820"
     CDS             5243..5566
                     /locus_tag="VHF05_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80086.1"
                     /translation="MATDQYHEPPQELSQEIRTFARMILSLIEEAEAIDWYEQRMSVE
                     KDDTAKAIMQNAQKEEFKHFGMDLEFLLRKKPVWRETLRGILFKEGDIVERGEEAEHK
                     ADKKE"
     gene            complement(5625..6707)
                     /locus_tag="VHF05_03825"
     CDS             complement(5625..6707)
                     /locus_tag="VHF05_03825"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVS80087.1"
                     /translation="MADDSNQKNHSNIKVTELPNSEVEIEGEVTAEKLEKARGKAVTR
                     LSQTLKIPGFRPGHTPEKVIIQHIGEYPILEDAAEIALTEEYPLILAEHGIDALGRPD
                     IAITKLAAGNPLGFKIKTAVNPRFALPDYKKIAKEKNVAHAAPDVSEKEIDEAIERIR
                     HNKAHLDLHKKLGTDLKNHEHEEIKAEDLPPLDDEFAKSIGDYATLADLRAKMKESLV
                     KEKEYEAREKTRIDIVESIIAATEIPLPEVLVDSELQKMLGQLKEDVSRTGLSYEEYL
                     KQINKSEDDLKKEWRETAEKKAKFQLILNKIADTEHIEPDMEVLEHESKQLMDMYKDA
                     DPMRIRVYVATLLINEAVLKFLEEQS"
     gene            6809..7465
                     /locus_tag="VHF05_03830"
     CDS             6809..7465
                     /locus_tag="VHF05_03830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVS80088.1"
                     /translation="MKFLSPLVSVTIDDGWKSAYTYALPLLKKHHAKATFYVITGAFD
                     EPHRLSRDQVRRLASIGHEIGAHTRNHLRLENVPSSREKRDQIVWCQDDLSDLGILAE
                     TFAYPYGEYDETYKRLIKEEGFTAARSVIRGYNDRTTDKFLLRVQGIRNTTSIEEMKG
                     WVDEVRRNSLWLILMFHQIDYSNSNFATTPELFDEILSYINLHWVEIDVVKNIAHLLG
                     "
     gene            complement(7524..7895)
                     /locus_tag="VHF05_03835"
     CDS             complement(7524..7895)
                     /locus_tag="VHF05_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80089.1"
                     /translation="MDNQVPNHMEQDMQQTAPQPQPPQPASDIPTAPTPRRRSSPYIP
                     AIVTIVILLIAALVGFYLYGLSLTMHQAALDQANQGAEASTSQDESGLQETSSIEQDL
                     NATDVDNSDAGLNNIDPNFQQ"
     gene            complement(7913..8653)
                     /locus_tag="VHF05_03840"
     CDS             complement(7913..8653)
                     /locus_tag="VHF05_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVS80090.1"
                     /translation="MTSKLAQRTAAVIAAVSFALAIPLGAFADMSANVSAGVGVSAEA
                     NSSNGITAAEHSAVRANAAASTTSEHPVAASVRALVTKLQAARGDFQTRIKEMRDKLL
                     NAKQEIKVKLNDRAKANVAAVLGAIITRFKNVETKLDNVSARLAANITDIENKKNIDL
                     SESTSLLADADAKLALAKGDVADAEADINAELNATTSKETLKADVETAKSSLKDAQEA
                     YLEVIKSIRTEVQAKVKATTTASADASL"
     gene            complement(8835..9377)
                     /locus_tag="VHF05_03845"
     CDS             complement(8835..9377)
                     /locus_tag="VHF05_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HVS80091.1"
                     /translation="MNLWHDIPRGEKIPDEFNAIIEIPKGSYNKYEIDKETGLIALDR
                     ANYSYAPYPFDYGLVPQTLWEDGDPLDVIVLTTFPLAPGILVKVRPVAVIDMIDSGDS
                     DYKVICVPVDDKRWDDTQDLADLNKHSLKEFRHFFETYKILKGKGDVVTVNGILGKAE
                     AVAAVKKSIELYEAKYGKKG"
     gene            9561..10226
                     /locus_tag="VHF05_03850"
     CDS             9561..10226
                     /locus_tag="VHF05_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVS80092.1"
                     /translation="MLDGSLKNVILKIEKEKGMLERMGFLSAAENRGSFFKRIALLFP
                     PLDPRYKAIERAYNCAKDAFRGVNREGGERYFEHIRAVVLILIDYLRVNDYRIIIAAL
                     LHDIVEDIPSWTIDRVRLEFGEFVARLVEYLTKPSKEEYSSKEERDLIYHSRFRFAPR
                     NFFLIKLADRLHNILTLGVCTPEKQQRKITETRMHYLPFAEKHLIFYHELIEAIERFQ
                     TSA"
     gene            complement(10308..11606)
                     /gene="nusA"
                     /locus_tag="VHF05_03855"
     CDS             complement(10308..11606)
                     /gene="nusA"
                     /locus_tag="VHF05_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HVS80093.1"
                     /translation="MLDLKVINAVLEQLEEERGIPREKVLEAIEMALATAYKKDYGKK
                     GQIVRAKFDLSSGKTEFYQVKYVVDDSMVYFDQEEEITDEVKTQGEEGSEKELFNPEK
                     HILLEDARRMKKDANLGDEIVFPLESKDDYGRIASQTAKQVIMQKIREAEKTSVFSEY
                     GQRQGEIVTGSVQRVERGNLFVDVGRATGILPYEEQIPGERYGQGERVRAYLYRVEET
                     PKGVFLRLSRSHPQFLKKLFETEAPEIVNGVVEIKAIAREAGYRSKVAVYSNDPHVDP
                     VGSLVGQRGVRVMTVMTELNNEKIDIIEWSADPKKFIEEALSPAKVISIDIDEEEKKA
                     LVRVSEDQQSLAIGKGGQNVRLAAKLTGWKIDIQSGDGAKPAESAEPLENEFIEEEAE
                     GNDDLTPPLPAESGSESGSGDDNEAAAGQDEVASGDGAAE"
     gene            complement(11935..13152)
                     /locus_tag="VHF05_03860"
     CDS             complement(11935..13152)
                     /locus_tag="VHF05_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVS80094.1"
                     /translation="MSNGTAVCVVKKNSRLHRPLDPTQTGREANSLDRTMKRMVVGQD
                     EAVEQIVNIYQMYLTGMTAPGRPIANFLFLGPTGTGKTRTVEALAETLVHNPRAVIKI
                     DCAEFQHSHEIAKLIGSPPGYLGHRETHPLLSQEVLNQFHTDTVKMSFVLFDEIEKAS
                     DALWNLLLGILDKAVLTLGDNRKVDFSHCMIFMTANLGVKEMENLTAPKHGFGRFDTS
                     QSSEISAATREKLAQKQSKTGVEAARRKFTPEFMNRIDKTVVFKPLGTTQLRRVLEIE
                     LGLVQQRALHSTADKSFVFTATGSAKDFLLAEGTDLKYGARHLKRAIERLLVQPLSNL
                     IATDQVHSGDWIKINFDGRKQCLKFTKEMEGLEVAAMANMLENTEEMPPPAASAVAPA
                     GTPTQLAKSSKKG"
     gene            13531..15327
                     /locus_tag="VHF05_03865"
     CDS             13531..15327
                     /locus_tag="VHF05_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVS80095.1"
                     /translation="MHIEETQLKDFILDSGLVSRADIAKAEEESAKSGVGLAKTLVGE
                     GKLTDDDVRRMQAYILGIPFVNLTNEHLDFSILSLIPEPIARKHNIVAFKKSDTTLEV
                     AMLDTEDLAAIDFMRKKVGLKILPRLTDSASMKHALLQYQKSLKAEFGDIIQKEAAHF
                     KNTEVPPASADGTALSEKEDGALSAATEGDLKKMAEDLPVVKIVETLLKHAILQNASD
                     IHIEPQENQMLVRYRIDGLLHDAMVLPLNIAESITARIKVLASLKLDEKRLPQDGRFK
                     IDMNGEKVSFRVSTLPTYYGEKTVMRVLRESVAGFTLEVLGFHGQGLEWIHKASKQTT
                     GMILTTGPTGSGKSTTLYTLLDILNEPTVNISTIEDPIEYQMKRVNQTQVKPEIGFSF
                     SAGLRTLVRQDPDIIMVGEIRDGETASLAVNASLTGHLVLSTLHTNSAAGAVPRLIDM
                     KIEPFLIVSTLDVVIGQRLVRKLEGEKEKYFLSKDEIKVLSDSVDLDRVLSFLVAENI
                     VEKDATWDKIPFYKPKAQGEGDDGYKGRMGIYEVLKMSSAIKELVMKGGTTQEIEDQA
                     KKEGMMTMIEDGIFKAVSGLTTIEEVLRVITE"
BASE COUNT         3405 a         3960 c         4369 g         3795 t
ORIGIN      
        1 taaaaataat tttcaatgtt tgaaggatat tcaccaaata ataatgagat cagaccgtct
       61 tctccggatc aagcagaaac cttcggaacg ctcgaaggag aggacgcttt tgagaaagcg
      121 atgtctcaga ttctcgacga tcttcccggc gcctccgata tcattcaacc tcgagactat
      181 caaggtattc tggaagaagc gaaggtgacg gaccaggaaa aaggcatatt ggaagaaatc
      241 gcgacgaagc tgccgacgat agaggaggcg cagattgcgc agcctatcca ggaaaaaata
      301 aagaacgcgg tgctcaagcg gctcgaggaa atggaggccg cggtgtaaac aaaaaacccg
      361 ctaaaagcgg gttttttgca taattagctc taattatcgg tcgaaagact gcgaatttgc
      421 agagaatttt cagattttgg atgcaattcg aggcgcgagc gaggtgagga agcgaaaact
      481 tgcagcttcc gcaaggcctt ttcaaatcga gtcttcgaga ttgaaaaggc gtaccgaacc
      541 tgtaaggttt gcgaccaagc tttcaacgaa gaagtgcgcc aaaatcgggg aattctactt
      601 tacggcgtct ttcaaagcct tagccggcct aaacttcgga actctcatcg aaggaacgga
      661 aatagtctct ccggtgcggg gattccgagc ctggcgcgaa gcgcgagtct ttgtcgagaa
      721 aatgcccaaa cccgcgatcg agacctcctc gcccttcttg agggtgttga tgatagcctc
      781 aacaacggtg tctaccgcct gctcagcctg gactttcgtt ccgccaagct tctcgtgcac
      841 cttctctaca atggcttgtt tgttcatatg cttgaactta atggttttta ggactttctc
      901 agtcggatgc aattcgaggc gcgagcgagg tgaggaagcg aaaacttgca gcttccgcaa
      961 ggccttttca aatcgagtct tcgagattga aaaggcgtac cgaacctgta aggtttgcga
     1021 ccaagctttc aacgaagaaa tgcgtcggct gtggaagtac taactgataa tcatgcatct
     1081 tgccgtacct ttcatcacag aatgagggcg tcggctagag gcgttcactc gataaccatt
     1141 atatagcaag gtatttttga tgcaagaaaa taaggattat tgacaaaatt gttgtttaaa
     1201 tttatttttt gatcaggaaa tcgcacggag aggagagctt tatggaaatt aggctgcaac
     1261 cccgtctcgt atacgaggtt tacaagtatt ctttctacgc attatgcgtg atgttcctat
     1321 gtatatgggc ggtaaccacc ctggtgcaga gcgtgcagga tgatctgcaa catatggaat
     1381 acggtctccg taccaatgca agccaaggaa cggcgacgtc gtacttggag ggaaactttg
     1441 gaccaaacga ctgaaggcaa atcagggcgc aggttcatga agatttgaaa tggcaaaggc
     1501 ggcgggaaat cccgccgctt tttcttaatg catgaatacg gcgcttgcgg ctatcgcgcg
     1561 aattcgatca cgcgggtttc gcggatgacg ctcactttga tttccccagg atatttgagc
     1621 tcgctttcga tgcgaagggc gatatcgcgt gccattttcc tcgcttcgag gtcggaaacg
     1681 gcgtcgggag tgacgaatac ccgaatttcg cgtcctgctt ggagtgcgta tgatttttcg
     1741 acgcctgcaa acgaattcgc ggtggcttcg aggtcggcga ggcgcttcag gtaattctcg
     1801 acagtgtcgc gccgcgcgcc cggcctgccg cccgagatcg cgtcggctac ctgcacgatg
     1861 atcgattcgg gagtttcgaa cggatattcg ccatggtgcg cctgcatcgc ctttatgatg
     1921 tcctcgctca cgttgaattt ctggagaatc ctgcggccga tctcgacgtg cgtgccggcc
     1981 acttcatggt cgagtgcctt gccgatgtcg tggagcaggg ctccggcctt tgcgacggcg
     2041 atattggcgc cgagctcttc ggcgagcatg ccagccaagt gcgacatttc caccgagtgc
     2101 tggaggacgt tctgtccgta acttgtcctg aaatagagcc gtcccaggat ggaaacgagc
     2161 cggggatcga gattatagac gccggcttcg aagacagccg cctcgccctt ttccttgatg
     2221 atcttgttga tttcctgctt ggatttttca actgtttcct cgatcttcgc gggctggatg
     2281 cggccgtcca aaacaaggtt ctcgagcgcg aggcgcgcga cctgcctgcg gacggggtcg
     2341 aagcaggaga gagtgatcgc gccgggagtg tcgtcgacga tcacttcgac gccggtcgcg
     2401 cgttcgaagg ccttgatgtt gcgcccttcc ttgccgatga tctttccctt gatctcgtca
     2461 ttgggaatga caaccgacgt cgtcataatg tcgggggaga ctgagttggc gaggcggtgg
     2521 atgacggtgg tgagaatatc cttggctttc cgttcgagct gttcggcgcc gaacgtctcg
     2581 agcttctgca agcggaccat caaatcctcc tcggcttctt tttcagcgat tgtttcgagt
     2641 tccttcttcg cttcgtccag ggagaacttg gcgacgcgct cgagctcctt ggttcgctct
     2701 gcgatgagat cgtcggctct ctgcctcaat tccttgattt tctcgatgcg ctccttgatg
     2761 tcttcgactt cgttgtcgat atcggtctgg cgcttgtcga gaagctcctc cttcttgatg
     2821 agacgctcct cggtgcgctt cagcttctcg tccttttcct tgatctcggc ccgggcttcg
     2881 cggaggagat cgtccgactt tttcgtcgct tcctcgagaa gcctcgcctc ctgctccttg
     2941 gcgtcgagca tgagggtctt gatgtcgagc tcgatcgaat tctttttctt gagggcgatg
     3001 aagattcgga gaaagtaccc gaaagcaatg cctgcaatgc ctgcggcaat gatgagtatt
     3061 acggttgttt ccatggtggg acgctgagct acttcgccga catatgggag ttcatgtcaa
     3121 gcttttggct atgcggaaag gtgcgccgat cgattttcat tcgacgttag gcacggagcg
     3181 tttgtttctg gggcaacaac ggtttgctgt gcgaaacctt gaagataaga ggggaattcg
     3241 gtcataaaag cggaagaatc tgagatctga cttaaaaatc ggccgacgga cggattaaat
     3301 gaaataaagt ggtacatata aaatatataa gaaatacggc gatatgtaaa ataccgctcg
     3361 gaattctgat tttaaagcga acaaaaagcc ccgcggaacg gcgattctgc gcgtcccgcg
     3421 aggcgttttt ggaaaaattt agatgatctt gtcgatgagg ccgtatttct tggcctcgtc
     3481 agcatcgagg aagtagtcgc ggtcgacgtc tttctcgatc tgggaaaccg gcttgcctgt
     3541 gttcttggcg agaatcttgt tgagctggtc gcggcttttc aagatctgct tggcggtgat
     3601 ctcgatatcg gtcgcctggc cgccgacgcc ctggatatga ggctggtgga tcatcacttc
     3661 ggaattgggg agcgagaagc gcttgccctt ggctcccgcc gagaggatga ccgccgcggc
     3721 tgaagcggcg ataccgacgc agaccgtcga gacgtcgggg gcgacgtggt tcatcgtgtc
     3781 gaggatcgcg agaccggccg taacggaacc tcccggggaa ttgatataga gcgaaatgtc
     3841 tttcttcgcg tcctcagatg cgaggaagag aagctgggcg atcacgatat tcgccgtatc
     3901 gtcgtcgatc gggccgccga ggaagatgat gcgctcccgc aaaagcctcg agtagatgtc
     3961 gtacgcgcgc tcgccgaact gcgacttttc gataactgtg ggaatgagat aagacatggg
     4021 ggaaaaaagt tgttagttta tagtcattag tttagagcaa gaaacggaaa caaaacagga
     4081 gctcgcgctt tcactaacgt cgaaagtata gcagaagggg agtacattga caatacataa
     4141 aaatcgttat aaattgcgga aggaggaaac aatgctttat tgcatggtag ttcggcagtc
     4201 taacgttacg ggcaagcatc ttgcatgcat tgctacattt accaccctga cacgattact
     4261 taacagtaac gacggagatg tcgtgttcgc gatagtgtgc gacactgaag atgttcaccc
     4321 gagcggagtc ttggaatttc ttaatgaaga ggtgcgtaaa accagctatg aatttgttcc
     4381 ggagatagga ttgggaatag aacctcccgt aacaatgatg ggagtcgagg cgggccgttc
     4441 cggataggag cggtcttttc ttttttatcg ttgtcacata ctatctacgc atgagactgc
     4501 acagattttt tatcgaccag gagatcaggg acgcgaagga tgttgtggtc acggatcgcg
     4561 accttctcaa tcagtggaaa aacgtattca ggctcggcaa ggaagaccgg gtcattcttc
     4621 tcgacggttc cggtttcgaa ttcgaggcgg aatttgcgct tcttgcccgt gatcgggccg
     4681 aactcgcgat tctcgcggtg aagaaggcgg cgaatgtttc cgaacggaac atcacgctct
     4741 acgcttcgct tatcaagaaa gataatttcg agtgggtgct tgagaaaggc accgaactcg
     4801 gcgtcgcggg gttcgtgccg cttcagacgg aacggagcga gaagaaggat ctcaatatcg
     4861 agcgcgcgaa gcggatcatc cgcgaagcga gcgagcaatc gggtagggga atggaacctg
     4921 agctttatgc cgtcgacacg cttgcagacg ctgtcgggaa cgcatcaatg aagcttgtcg
     4981 cgttcgatcc gacaggcgcc gagctcgata gaaactctct gcgggaaagt gaaatcggaa
     5041 ttctcgtcgg cccggaaggc ggctggagcg agcgcgaact cgcgtttttc aaggaaaaga
     5101 atatttccgt ctacacgctg ggaccgcagg tattgcgggc ggaaaccgcg gcgatcgcca
     5161 tctccacgct cgcgcttctc taggaattca tcgaaagctc aatttgtgcg cctatgctga
     5221 agggaaataa ctctcaacag ccatggcaac ggaccaatat cacgaaccgc cgcaggagct
     5281 ttcccaggag atccgcacct ttgcgcggat gattctttcg ctcatcgaag aagcagaggc
     5341 tatcgactgg tacgaacagc ggatgtcggt ggagaaagac gatacggcca aggcgatcat
     5401 gcaaaacgcc cagaaggaag agttcaagca cttcggcatg gatctcgagt tcctgctccg
     5461 caagaagccc gtctggagag agacgctccg agggattctc ttcaaggagg gcgatattgt
     5521 ggaacgcggt gaagaagctg agcataaagc ggataagaaa gagtgagtca acaaaaaacc
     5581 ggctagtgcc ggtttttgct tggacttcga tttgaatttg ttgcttagga ctgttcctcg
     5641 agaaatttga ggaccgcttc gttgatgaga agagtggcga catagacgcg gattcgcatc
     5701 gggtcggcat ctttgtacat gtccatgagc tgtttcgatt catgctcgag cacctccatg
     5761 tccggctcga tatgctcggt atcggcgatt ttgttcaaaa tgagctggaa tttcgccttc
     5821 ttttccgccg tctcgcgcca ttccttcttg agatcatcct cgctcttatt gatctgcttc
     5881 agatactctt cgtatgagag tcccgtgcgg ctgacatcct ctttgagctg gccgagcatt
     5941 ttctgaagct cgctgtcgac gagcacctcg ggaagcggga tctccgtcgc ggcgatgatc
     6001 gactcaacga tgtcgatgcg cgtcttttcg cgcgcctcgt attccttctc cttgacgagg
     6061 ctttccttca tcttggcgcg aaggtcggcg agcgtggcgt agtcgccgat cgacttggcg
     6121 aattcatcgt cgaggggcgg caggtcttca gctttgattt cctcgtgctc gtggtttttc
     6181 aggtcggtgc cgagcttctt gtggaggtcg agatgggctt tgttgtggcg aatgcgctct
     6241 atcgcctcgt cgatttcctt ttcgctcacg tcgggcgccg catgcgcgac gttcttttcc
     6301 ttggcgattt tcttgtagtc gggaagcgcg aagcggggat tgaccgcggt cttgatcttg
     6361 aagcccagcg gattgccggc ggcgagcttg gtgatcgcga tatcgggacg gccgagggcg
     6421 tcgatgccgt gctcggcaag aataagcggg tactcctcgg taagggcgat ctccgccgcg
     6481 tcctcgagaa tcgggtattc tccgatgtgc tggatgatca ccttctcagg agtgtggccg
     6541 ggcctgaagc ccgggatctt caaggtctgc gaaaggcgcg tcaccgcttt tccgcgcgcc
     6601 ttctcgagct tttcggctgt cacttcgccc tcgatctcga cttcggagtt ggggagctcc
     6661 gtaaccttga tattcgagtg atttttctgg ttggaatcgt ctgccattgg atatatgaaa
     6721 ttaatgcttc cttagagtat caagattttc ccatttggca agaaatgagg tataaaagag
     6781 cagttcgaag ttctttaaag gagacattat gaaatttcta tcgccgctcg tctctgtcac
     6841 cattgatgac ggctggaaaa gcgcctatac atacgcactt cctttgctta agaaacatca
     6901 tgccaaagcg acattctatg tgatcaccgg cgcgttcgac gaaccgcacc ggctctcgcg
     6961 cgatcaagtg aggagactgg ccagtatcgg ccatgagatc ggagctcata cgcgcaatca
     7021 cctacggctg gaaaacgtgc cctcttcccg ggagaagcgt gatcaaatcg tgtggtgcca
     7081 ggatgatctg agcgatctcg gcatattggc agaaaccttc gcatatccct acggggaata
     7141 tgacgaaaca tacaaaagac tgataaaaga agagggattt accgctgccc gaagtgttat
     7201 cagagggtac aatgatcgca caaccgataa atttctcctc cgggttcagg gaatccgcaa
     7261 caccaccagt attgaggaaa tgaaaggatg ggtcgacgag gtacggcgaa actctctttg
     7321 gcttatccta atgttccatc agatcgatta ttcgaacagc aactttgcga ccacgccgga
     7381 attattcgac gagattctca gctatatcaa tctgcactgg gtggagatcg atgttgtgaa
     7441 aaatatcgcg catctacttg gttagaaaag aaagggcggc acgcgttgcg tgccgccctc
     7501 tatctttggt ttttcttatt ccgctactgc tggaaattcg gatcgatgtt gttgaggccg
     7561 gcgtcggaat tgtcgacatc ggtcgcattg agatcctgct cgattgacga cgtctcctga
     7621 aggcccgatt cgtcctgcga cgtcgacgct tccgcgccct ggttcgcttg gtcgagcgcc
     7681 gcctggtgca tagtgagcga gaggccgtag agatagaagc cgacgagcgc ggcgataaga
     7741 aggatgacga tcgtcacgat tgccggaatg tacggcgagg agcggcggcg gggagtcggc
     7801 gcggtgggaa tgtctgaagc cggctgcggc ggctggggtt ggggagcggt ttgctgcata
     7861 tcctgctcca tgtgattggg aacttgattg tccatacgaa ttgattaggt gattataacg
     7921 aagcgtccgc cgatgcggtc gtcgtagctt ttacttttgc ctgtacttcg gtgcggatcg
     7981 atttgatgac ctcgaggtac gcttcctgcg catccttgag gctcgacttc gcggtctcga
     8041 cgtcggcttt cagggtctct ttcgaggtcg tcgcattgag ctccgcgttt atgtccgctt
     8101 cggcgtcagc tacatctccc ttcgcgagcg cgagcttggc atccgcatcg gcaagaagcg
     8161 atgtgctctc cgaaaggtcg atgttcttct tattctcgat atcagtgatg ttcgcggcga
     8221 ggcgcgcgct gacgttgtcg agcttcgtct cgacgttctt gaagcgggtg atgatcgcgc
     8281 cgaggacggc tgctacgttt gccttggccc tatcgttcag cttcacctta atttcctgct
     8341 tggcgttcag gagcttgtcg cgcatttcct tgatgcgggt ttggaaatct ccccgcgccg
     8401 cctggagttt cgttacgagt gcacgcacgc ttgcggcaac ggggtgctcg ctcgtggtgc
     8461 ttgccgccgc atttgccctt acggcgctat gctctgccgc cgtgatccca ttcgaagaat
     8521 tcgcttccgc cgaaacgcct acgcctgcgg atacgttcgc agacatatcg gcgaacgctc
     8581 cgagcgggat agcgagggcg aaggagacgg ccgctataac cgctgcggtc cgctgtgcta
     8641 gcttgctagt catgtggttg gattgaaaaa aattatctat taatctctta cggcgcaccg
     8701 cgccgctcca taccactcta ttatatatga aatgcggcag aggcttcccc ggcttctgca
     8761 cagggaaaag acaagggccg tcaggcgcca gaagggcgcc tgacggccct ttttcatatg
     8821 ctggagcttt tgtcctaacc tttctttccg tacttcgctt cgtagagctc gatcgatttt
     8881 ttgactgccg cgaccgcttc cgccttgccg aggatgccgt ttaccgtaac aacatcgccc
     8941 ttgcccttca aaatcttgta tgtctcgaaa aaatgccgga attccttgag agagtgcttg
     9001 ttcaagtccg cgaggtcctg cgtgtcgtcc cagcgcttgt cgtcgaccgg tacgcagatc
     9061 accttatagt cggaatcgcc ggaatcgatc atgtcgatta ccgccacggg acggactttg
     9121 acgaggatgc cgggcgccaa cgggaacgtc gtgaggacga tgacgtccag gggatcgccg
     9181 tcttcccaca gcgtctgcgg tacgaggccg tagtcgaacg ggtagggggc gtaggaataa
     9241 ttggcgcggt cgagggcgat caggccggtt tccttgtcga tctcgtattt gttgtacgag
     9301 cccttgggga tttcgatgat ggcgttgaac tcatcgggaa tcttctcgcc gcgaggaatg
     9361 tcgtgccaga ggttcataag ataggtgtta gcttatagct tttagctgtt agtaagagta
     9421 agacactagg aaatgtagca tcttctgccg taattttcaa ttttgaattt ttaattttca
     9481 atcaatatcc atgactcaat tttcaaatct gtagattatt tattttgagt catgggacaa
     9541 gttttcacgg cagctcacaa atgcttgacg gttctcttaa aaatgttatt ttaaaaatag
     9601 aaaaggagaa agggatgctt gaacgcatgg gttttttatc tgcagctgaa aacaggggga
     9661 gtttttttaa gagaattgcc ttgctgtttc cacccctcga tcctcgttat aaggcgattg
     9721 agagagcgta caattgcgcc aaagacgcct ttaggggagt taacagggaa ggcggcgaga
     9781 ggtattttga gcatattcga gccgtagtgc tcatacttat cgattatttg cgggttaatg
     9841 attatcgtat aatcattgcc gcactgctcc acgatatagt tgaggatata ccgtcctgga
     9901 caatcgatcg tgtcagattg gaattcggcg aattcgtggc ccggcttgtt gaatatctta
     9961 caaagccttc caaggaggaa tattcatcta aggaagagag ggatcttatc tatcattcgc
    10021 gattccgttt cgcgccgcga aatttcttcc tgatcaagct cgcggacagg ctccacaata
    10081 ttcttacgct cggcgtctgt actcctgaga aacaacaaag aaaaatcact gagactcgga
    10141 tgcactatct gccttttgca gaaaagcatc tgatcttcta tcacgaattg atcgaagcaa
    10201 ttgagcgttt tcaaacatca gcataaggcc aatcaagagc caatctttcg tatcataaaa
    10261 gcaccggaca gccggtgctt tctttattgg taggaatttt tgaatgatta ctcggccgcg
    10321 ccgtcgccgc ttgcgacctc gtcctgtcct gcggccgctt cgttgtcgtc gccggagccg
    10381 ctttcgctgc ctgattccgc gggaagcggg ggagtgagat catcattgcc ctcggcttcc
    10441 tcctcgatga attcgttctc aagaggctct gcgctttccg ccggcttggc gccgtcgccg
    10501 gactggatgt cgatcttcca gccggtgagc ttcgcggcga ggcgcacgtt ctggcctccc
    10561 ttgccgatgg cgagggactg ctggtcttcg gaaacgcgca cgagcgcctt cttctcctcc
    10621 tcatcgatgt cgatggagat gaccttcgcg ggcgagagcg cctcctcgat gaatttcttc
    10681 gggtcggcgg accattcgat gatgtcgatc ttctcgttgt tgagctcggt catgacggtc
    10741 atgacgcgca cacccctctg tccgacgagc gagccgacag ggtcgacgtg cggatcgttg
    10801 gaatagacgg cgaccttcga gcggtagccc gcctcgcggg cgatcgcctt gatctccacg
    10861 acgccgttca cgatctcggg cgcctcggtt tcgaagagct tcttgaggaa ttgcggatgc
    10921 gagcgggaga ggcggagaaa tacgcccttc ggggtttcct cgacgcggta aaggtaggcg
    10981 cggacgcgct cgccctggcc gtagcgctcg ccggggatct gctcctcata agggaggatg
    11041 ccggtggcgc ggcccacgtc gacgaagaga ttgccgcgct cgacgcgctg gaccgagccg
    11101 gtgacgatct ccccctggcg ctggccgtat tcgctgaaga ccgaggtctt ctcggcctcg
    11161 cggatcttct gcatgattac ctgcttcgcg gtctgcgacg cgatgcggcc gtagtcgtcc
    11221 ttgctctcga ggggaaagac gatctcgtcg ccgagattgg cgtccttctt catgcggcgc
    11281 gcgtcctcga gaagaatatg cttctcggga ttgaaaagct ccttttccga gccttcctcc
    11341 ccctgggttt tcacttcgtc ggtgatttcc tcttcctggt cgaaatacac catcgagtca
    11401 tcgacgacgt atttcacctg gtagaattcg gtcttgccgc tactcaggtc gaacttggcg
    11461 cggacgatct ggcctttttt gccgtagtcc ttcttgtacg cggtggcgag cgccatttcg
    11521 atcgcttcga ggactttctc gcgcgggatg ccgcgctcct cttcgagctg ttccaagacc
    11581 gcatttatta cttttagatc caacatgggt gattttctta tgagcgaagc gaatttgaaa
    11641 atctccaccc agcaccattt ctgaatccga cgataactga tcacttaaac gggtaagagc
    11701 agttatttgc tcgattgccg acaagtttcc gtatcagaaa aggtgctggg ttaagaaaat
    11761 tatattcact gcgttcatta attttctaaa aaaatagccc gccgccggat ttccgaaccg
    11821 gggcgaactg acatgctggc attataccaa actagatttt agtgtcaagt caagagcgga
    11881 aagaaacgaa agaaaaaagc ggcgctccgg agagcaccgc ttatagtata agcgtcatcc
    11941 ttttttggat gactttgcga gctgggtagg tgttcctgcc ggggcaacgg cagatgccgc
    12001 aggaggcggc atttcctcgg tgttttcgag catgttcgcc atggcggcaa cctcgaggcc
    12061 ttccatttcc tttgtgaatt taaggcactg ctttcttcca tcgaagttga tcttgatcca
    12121 gtcgccggaa tgaacctgat cggtggcgat gaggtttgag agcggctgga cgagcaggcg
    12181 ctcgatggcg cgcttgaggt gccgcgcgcc gtacttgagg tccgtgcctt cggcgaggag
    12241 gaaatccttg gccgagccgg tggcggtaaa gacaaatgat ttatcggcgg tcgaatggag
    12301 cgcgcgctgc tgcacgagcc cgagttcgat ttcgagcacg cggcgcaatt gtgtcgtgcc
    12361 gagcggcttg aacactacag tcttatcgat gcggttcatg aattccggcg tgaacttgcg
    12421 gcgcgctgct tccactccgg ttttcgattg tttttgggcg agtttttccc gcgttgccgc
    12481 tgaaatctcg gacgattgcg acgtgtcgaa tcgtccgaag ccgtgcttgg gcgccgtgag
    12541 attttccatc tccttgacgc cgagattggc ggtcatgaag atcatgcagt gcgagaaatc
    12601 gaccttgcga ttgtcgccga gcgtcagtac cgccttgtcg agaatcccca aaaggagatt
    12661 ccagagggcg tccgacgcct tttcgatctc gtcgaagagg acgaagctca tcttcacggt
    12721 gtcggtgtgg aattggttga gcacttcttg gctcaggagg ggatgggttt cgcgatggcc
    12781 caggtatccg ggcggcgagc cgatgagctt tgcgatctca tggctgtgct ggaattcggc
    12841 gcagtcgatc ttgatgaccg cgcgaggatt gtgcacgagc gtttcggcga gcgcttctac
    12901 cgtgcgagtc tttccggtgc ccgtcgggcc caggaagagg aagtttgcga tcgggcgtcc
    12961 tggcgcggtc atgcccgtca ggtacatctg atatatgttg acgatctgct cgacggcttc
    13021 gtcctggccg acgaccattc tcttcatcgt tcggtcaagc gaattcgcct cccgtcccgt
    13081 ttgcgtcgga tcgagcggcc ggtgaaggcg ggaatttttc tttaccacgc acacagctgt
    13141 cccattgctc attttttact gcctcctttt gcagcttttt caaataacaa atttcaaagc
    13201 tcatacgttg agccgggaaa agcaagctct attccatgct tctactttca ttagattccc
    13261 acttccgccg attgtcaatg aagcgcttgg cgtgcgcccg tgagagtgtg catagcgaaa
    13321 aaccgccgcg ttttctgata taatttaaga aaataatgag cgaagcgagt gaagatattt
    13381 tcttaaccca gcaccatttc tgatatggaa gtatgccagg aatcagacgc taaacatgct
    13441 tttccatgtt tgagtggcca gtaatcgtcg gtttcagaaa tggtgttggg tggcgatttt
    13501 tataatcgcc ccgctcatta aaaatcagcc atgcacatcg aagagacaca actcaaggac
    13561 ttcattctcg actccgggct tgtgagccgc gccgatatcg ccaaggccga ggaagagtcc
    13621 gcgaaatcgg gcgtcgggct cgccaagacg cttgtcgggg aggggaagct taccgatgac
    13681 gacgtgcgcc gcatgcaggc gtacatcctc ggcattccct tcgtcaatct caccaacgag
    13741 catctcgact tcagcatcct ctcgcttatt cccgagccga tcgcccgcaa gcacaacatc
    13801 gtcgccttca agaaaagcga tacgacgctc gaggtggcga tgctcgacac ggaagacctc
    13861 gcggcgatcg acttcatgcg caagaaggtc ggcctcaaaa tcctgccgcg cctcaccgat
    13921 tccgcatcga tgaagcatgc gcttctccag taccagaaga gcctcaaggc cgaattcggc
    13981 gacatcatcc agaaggaagc cgcgcatttc aagaataccg aggttccccc cgcttccgcg
    14041 gacggcacgg cgctctccga aaaggaggac ggcgctttga gcgccgcaac cgaaggcgac
    14101 ctgaagaaaa tggcggaaga tcttcccgtg gtgaagatcg tcgagacgct cctgaagcat
    14161 gccattctcc agaacgcgtc ggacatccac atcgagccgc aggaaaacca gatgctcgtc
    14221 cgctatcgca tcgacggcct cctccatgac gcaatggtcc ttcctttgaa tatcgccgag
    14281 agcatcactg cgcgcatcaa ggtgcttgcg tccttgaagc tcgacgagaa gcgcctgccg
    14341 caggacggcc gtttcaagat cgacatgaac ggcgagaagg tttccttccg cgtgtcgacc
    14401 ctgccgacct actacggcga gaagaccgtg atgcgcgttc tccgcgaatc ggtcgccggc
    14461 ttcacgctcg aagttctggg ctttcatggc caggggctcg agtggatcca caaggcgtcg
    14521 aagcagacga ccggcatgat tttgaccacc ggtccgacgg gctccggaaa gtcgacgacg
    14581 ctttacacgc tcctcgatat cctgaacgag ccgacggtga atatttcgac catcgaggat
    14641 ccgatcgagt atcagatgaa gcgcgtcaac cagacgcagg taaagccgga gatcggcttc
    14701 tcgttctcgg ccggcttgcg caccttggtg cgccaggatc cggacatcat catggtcggc
    14761 gagattcgcg acggcgaaac cgcctcgctc gccgtgaacg cgtcgctcac aggccacctc
    14821 gtgctctcga ccctccatac caactccgcc gcgggcgccg tgccgcgact catcgacatg
    14881 aagatcgagc cgttcctcat cgtgtcgacc ctggacgttg tcatcggcca gcgcctcgtc
    14941 cgcaagctcg aaggggagaa ggagaaatat ttcctttcca aggatgagat caaggtgctc
    15001 tccgactccg tcgatctcga ccgcgtgctc tcgtttctcg tcgcggaaaa catcgtcgag
    15061 aaggatgcga cgtgggacaa gattccgttc tacaagccga aggcgcaggg cgagggcgat
    15121 gacggctaca aggggcgcat gggcatctac gaagtgctca agatgtcttc cgcaatcaag
    15181 gagcttgtca tgaagggcgg caccacgcag gagatcgagg accaggccaa gaaggagggc
    15241 atgatgacca tgatcgagga cggcatcttc aaggcggttt ccggccttac cacgatcgag
    15301 gaagtgctgc gcgttatcac ggaatagcga actcaaaact caaagatcaa aatgcaaaaa
    15361 tataatgcaa aaccaacaat ggaagacaaa ttgaagaaaa aattcatttg gatcccctgc
    15421 tggggatgac gaaggaccct caattttgac ctttgagttt taacttttga cttattaacg
    15481 tgaatttcaa atacaaagcg gcgaaaaacg gtgccctcga agaaaatac
//
